LOCUS       DACDRN010000001       159877 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDRN010000001 DACDRN010000000
VERSION     DACDRN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 159877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 159877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..159877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.001"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            216..848
                     /locus_tag="IWJ80_000001"
     CDS             216..848
                     /locus_tag="IWJ80_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9459777.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            971..1609
                     /locus_tag="IWJ80_000002"
     CDS             971..1609
                     /locus_tag="IWJ80_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9459778.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(1728..1800)
                     /locus_tag="IWJ80_000003"
     tRNA            complement(1728..1800)
                     /locus_tag="IWJ80_000003"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1765..1767),aa:Thr,seq:cgt)
     gene            1921..2163
                     /locus_tag="IWJ80_000004"
     CDS             1921..2163
                     /locus_tag="IWJ80_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9459779.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            complement(2302..2931)
                     /gene="upp"
                     /locus_tag="IWJ80_000005"
     CDS             complement(2302..2931)
                     /gene="upp"
                     /locus_tag="IWJ80_000005"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP9459780.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(3065..4012)
                     /locus_tag="IWJ80_000006"
     CDS             complement(3065..4012)
                     /locus_tag="IWJ80_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9459781.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            complement(4165..5409)
                     /locus_tag="IWJ80_000007"
     CDS             complement(4165..5409)
                     /locus_tag="IWJ80_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP9459782.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            complement(5584..6609)
                     /locus_tag="IWJ80_000008"
     CDS             complement(5584..6609)
                     /locus_tag="IWJ80_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP9459783.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            complement(6631..6810)
                     /locus_tag="IWJ80_000009"
     CDS             complement(6631..6810)
                     /locus_tag="IWJ80_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9459784.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            complement(6803..7642)
                     /gene="prmC"
                     /locus_tag="IWJ80_000010"
     CDS             complement(6803..7642)
                     /gene="prmC"
                     /locus_tag="IWJ80_000010"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP9459785.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            complement(7635..8708)
                     /gene="prfA"
                     /locus_tag="IWJ80_000011"
     CDS             complement(7635..8708)
                     /gene="prfA"
                     /locus_tag="IWJ80_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP9459786.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            complement(8711..9295)
                     /locus_tag="IWJ80_000012"
     CDS             complement(8711..9295)
                     /locus_tag="IWJ80_000012"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP9459787.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            complement(9401..9870)
                     /locus_tag="IWJ80_000013"
                     /pseudo
     CDS             complement(9401..9870)
                     /locus_tag="IWJ80_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            9976..10578
                     /locus_tag="IWJ80_000014"
     CDS             9976..10578
                     /locus_tag="IWJ80_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9459788.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            complement(10682..11251)
                     /gene="lepB"
                     /locus_tag="IWJ80_000015"
     CDS             complement(10682..11251)
                     /gene="lepB"
                     /locus_tag="IWJ80_000015"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9459789.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(11481..12248)
                     /locus_tag="IWJ80_000016"
     CDS             complement(11481..12248)
                     /locus_tag="IWJ80_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9459790.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            complement(12245..12724)
                     /locus_tag="IWJ80_000017"
     CDS             complement(12245..12724)
                     /locus_tag="IWJ80_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP9459791.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            complement(12849..13586)
                     /locus_tag="IWJ80_000018"
     CDS             complement(12849..13586)
                     /locus_tag="IWJ80_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP9459792.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            13747..15147
                     /locus_tag="IWJ80_000019"
     CDS             13747..15147
                     /locus_tag="IWJ80_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP9459793.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            15370..16722
                     /locus_tag="IWJ80_000020"
     CDS             15370..16722
                     /locus_tag="IWJ80_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP9459794.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            16715..17413
                     /locus_tag="IWJ80_000021"
     CDS             16715..17413
                     /locus_tag="IWJ80_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP9459795.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(17475..18626)
                     /locus_tag="IWJ80_000022"
     CDS             complement(17475..18626)
                     /locus_tag="IWJ80_000022"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP9459796.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(18639..19076)
                     /locus_tag="IWJ80_000023"
     CDS             complement(18639..19076)
                     /locus_tag="IWJ80_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9459797.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(19091..20857)
                     /locus_tag="IWJ80_000024"
     CDS             complement(19091..20857)
                     /locus_tag="IWJ80_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP9459798.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            21121..21798
                     /locus_tag="IWJ80_000025"
     CDS             21121..21798
                     /locus_tag="IWJ80_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9459799.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            21801..22928
                     /locus_tag="IWJ80_000026"
     CDS             21801..22928
                     /locus_tag="IWJ80_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9459800.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(23128..23568)
                     /locus_tag="IWJ80_000027"
     CDS             complement(23128..23568)
                     /locus_tag="IWJ80_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9459801.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(23579..25612)
                     /locus_tag="IWJ80_000028"
     CDS             complement(23579..25612)
                     /locus_tag="IWJ80_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9459802.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(25614..27317)
                     /locus_tag="IWJ80_000029"
     CDS             complement(25614..27317)
                     /locus_tag="IWJ80_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9459803.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(27411..28361)
                     /gene="manA"
                     /locus_tag="IWJ80_000030"
     CDS             complement(27411..28361)
                     /gene="manA"
                     /locus_tag="IWJ80_000030"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP9459804.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(28400..29425)
                     /locus_tag="IWJ80_000031"
     CDS             complement(28400..29425)
                     /locus_tag="IWJ80_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9459805.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(29634..30503)
                     /locus_tag="IWJ80_000032"
     CDS             complement(29634..30503)
                     /locus_tag="IWJ80_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP9459806.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(31207..33099)
                     /locus_tag="IWJ80_000033"
     CDS             complement(31207..33099)
                     /locus_tag="IWJ80_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9459807.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(33096..34823)
                     /locus_tag="IWJ80_000034"
     CDS             complement(33096..34823)
                     /locus_tag="IWJ80_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9459808.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(34839..35489)
                     /locus_tag="IWJ80_000035"
     CDS             complement(34839..35489)
                     /locus_tag="IWJ80_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9459809.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            35693..35989
                     /locus_tag="IWJ80_000036"
     CDS             35693..35989
                     /locus_tag="IWJ80_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9459810.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            36181..36801
                     /locus_tag="IWJ80_000037"
     CDS             36181..36801
                     /locus_tag="IWJ80_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP9459811.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(36893..38059)
                     /locus_tag="IWJ80_000038"
     CDS             complement(36893..38059)
                     /locus_tag="IWJ80_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9459812.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(38071..38424)
                     /locus_tag="IWJ80_000039"
     CDS             complement(38071..38424)
                     /locus_tag="IWJ80_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9459813.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            38552..39574
                     /locus_tag="IWJ80_000040"
     CDS             38552..39574
                     /locus_tag="IWJ80_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP9459814.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(40013..40447)
                     /locus_tag="IWJ80_000041"
     CDS             complement(40013..40447)
                     /locus_tag="IWJ80_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9459815.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(40557..42416)
                     /locus_tag="IWJ80_000042"
     CDS             complement(40557..42416)
                     /locus_tag="IWJ80_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9459816.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(42534..43694)
                     /locus_tag="IWJ80_000043"
     CDS             complement(42534..43694)
                     /locus_tag="IWJ80_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP9459817.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(44001..45425)
                     /gene="araA"
                     /locus_tag="IWJ80_000044"
     CDS             complement(44001..45425)
                     /gene="araA"
                     /locus_tag="IWJ80_000044"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP9459818.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(45441..46139)
                     /locus_tag="IWJ80_000045"
     CDS             complement(45441..46139)
                     /locus_tag="IWJ80_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9459819.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(46096..47757)
                     /locus_tag="IWJ80_000046"
     CDS             complement(46096..47757)
                     /locus_tag="IWJ80_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP9459820.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            47975..49375
                     /locus_tag="IWJ80_000047"
     CDS             47975..49375
                     /locus_tag="IWJ80_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP9459821.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(49627..50706)
                     /locus_tag="IWJ80_000048"
     CDS             complement(49627..50706)
                     /locus_tag="IWJ80_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9459822.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(50792..51751)
                     /locus_tag="IWJ80_000049"
     CDS             complement(50792..51751)
                     /locus_tag="IWJ80_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP9459823.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(51763..53286)
                     /locus_tag="IWJ80_000050"
     CDS             complement(51763..53286)
                     /locus_tag="IWJ80_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9459824.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(53296..53859)
                     /locus_tag="IWJ80_000051"
     CDS             complement(53296..53859)
                     /locus_tag="IWJ80_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP9459825.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(53865..54728)
                     /locus_tag="IWJ80_000052"
     CDS             complement(53865..54728)
                     /locus_tag="IWJ80_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9459826.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(54742..55638)
                     /locus_tag="IWJ80_000053"
     CDS             complement(54742..55638)
                     /locus_tag="IWJ80_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9459827.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(55813..57084)
                     /locus_tag="IWJ80_000054"
     CDS             complement(55813..57084)
                     /locus_tag="IWJ80_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9459828.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(57074..58591)
                     /locus_tag="IWJ80_000055"
     CDS             complement(57074..58591)
                     /locus_tag="IWJ80_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9459829.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            58789..59679
                     /locus_tag="IWJ80_000056"
     CDS             58789..59679
                     /locus_tag="IWJ80_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP9459830.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(59752..60576)
                     /gene="nadE"
                     /locus_tag="IWJ80_000057"
     CDS             complement(59752..60576)
                     /gene="nadE"
                     /locus_tag="IWJ80_000057"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP9459831.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(60588..62060)
                     /locus_tag="IWJ80_000058"
     CDS             complement(60588..62060)
                     /locus_tag="IWJ80_000058"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP9459832.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(62697..63059)
                     /locus_tag="IWJ80_000059"
     CDS             complement(62697..63059)
                     /locus_tag="IWJ80_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9459833.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            63282..64034
                     /locus_tag="IWJ80_000060"
     CDS             63282..64034
                     /locus_tag="IWJ80_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9459834.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(64174..65097)
                     /locus_tag="IWJ80_000061"
     CDS             complement(64174..65097)
                     /locus_tag="IWJ80_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9459835.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(65112..65384)
                     /locus_tag="IWJ80_000062"
     CDS             complement(65112..65384)
                     /locus_tag="IWJ80_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9459836.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(65728..66915)
                     /locus_tag="IWJ80_000063"
     CDS             complement(65728..66915)
                     /locus_tag="IWJ80_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP9459837.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            67074..68198
                     /locus_tag="IWJ80_000064"
     CDS             67074..68198
                     /locus_tag="IWJ80_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9459838.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            68425..69342
                     /locus_tag="IWJ80_000065"
     CDS             68425..69342
                     /locus_tag="IWJ80_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9459839.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            69335..70168
                     /locus_tag="IWJ80_000066"
     CDS             69335..70168
                     /locus_tag="IWJ80_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9459840.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            70251..71369
                     /locus_tag="IWJ80_000067"
     CDS             70251..71369
                     /locus_tag="IWJ80_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9459841.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            71363..73261
                     /locus_tag="IWJ80_000068"
     CDS             71363..73261
                     /locus_tag="IWJ80_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP9459842.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            73255..74478
                     /locus_tag="IWJ80_000069"
     CDS             73255..74478
                     /locus_tag="IWJ80_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP9459843.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(74564..76498)
                     /locus_tag="IWJ80_000070"
     CDS             complement(74564..76498)
                     /locus_tag="IWJ80_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9459844.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            76724..77380
                     /locus_tag="IWJ80_000071"
     CDS             76724..77380
                     /locus_tag="IWJ80_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9459845.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(77454..77807)
                     /locus_tag="IWJ80_000072"
     CDS             complement(77454..77807)
                     /locus_tag="IWJ80_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9459846.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            77999..78928
                     /locus_tag="IWJ80_000073"
     CDS             77999..78928
                     /locus_tag="IWJ80_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP9459847.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            78939..79775
                     /locus_tag="IWJ80_000074"
     CDS             78939..79775
                     /locus_tag="IWJ80_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP9459848.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            80023..80799
                     /locus_tag="IWJ80_000075"
     CDS             80023..80799
                     /locus_tag="IWJ80_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9459849.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            80975..81718
                     /locus_tag="IWJ80_000076"
     CDS             80975..81718
                     /locus_tag="IWJ80_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9459850.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            81711..83291
                     /locus_tag="IWJ80_000077"
     CDS             81711..83291
                     /locus_tag="IWJ80_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP9459851.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(83542..84021)
                     /locus_tag="IWJ80_000078"
     CDS             complement(83542..84021)
                     /locus_tag="IWJ80_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP9459852.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(84037..84789)
                     /locus_tag="IWJ80_000079"
     CDS             complement(84037..84789)
                     /locus_tag="IWJ80_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP9459853.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            84906..84976
                     /locus_tag="IWJ80_000080"
     tRNA            84906..84976
                     /locus_tag="IWJ80_000080"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:84938..84940,aa:Gly,seq:ccc)
     gene            85337..86062
                     /locus_tag="IWJ80_000081"
     CDS             85337..86062
                     /locus_tag="IWJ80_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP9459854.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            86317..86583
                     /locus_tag="IWJ80_000082"
     CDS             86317..86583
                     /locus_tag="IWJ80_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP9459855.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            86580..87011
                     /gene="ruvX"
                     /locus_tag="IWJ80_000083"
     CDS             86580..87011
                     /gene="ruvX"
                     /locus_tag="IWJ80_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP9459856.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            87036..87341
                     /locus_tag="IWJ80_000084"
     CDS             87036..87341
                     /locus_tag="IWJ80_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP9459857.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(87422..88567)
                     /locus_tag="IWJ80_000085"
     CDS             complement(87422..88567)
                     /locus_tag="IWJ80_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9459858.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(88628..89275)
                     /locus_tag="IWJ80_000086"
     CDS             complement(88628..89275)
                     /locus_tag="IWJ80_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9459859.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            89469..89762
                     /locus_tag="IWJ80_000087"
     CDS             89469..89762
                     /locus_tag="IWJ80_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9459860.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            89806..90117
                     /locus_tag="IWJ80_000088"
     CDS             89806..90117
                     /locus_tag="IWJ80_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9459861.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            90114..90738
                     /locus_tag="IWJ80_000089"
                     /pseudo
     CDS             90114..90738
                     /locus_tag="IWJ80_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            91038..91400
                     /locus_tag="IWJ80_000090"
     CDS             91038..91400
                     /locus_tag="IWJ80_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9459862.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            91437..92285
                     /locus_tag="IWJ80_000091"
     CDS             91437..92285
                     /locus_tag="IWJ80_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP9459863.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            92275..93750
                     /locus_tag="IWJ80_000092"
     CDS             92275..93750
                     /locus_tag="IWJ80_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP9459864.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            94016..94423
                     /locus_tag="IWJ80_000093"
     CDS             94016..94423
                     /locus_tag="IWJ80_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP9459865.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            94426..94641
                     /locus_tag="IWJ80_000094"
     CDS             94426..94641
                     /locus_tag="IWJ80_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9459866.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            94645..95025
                     /locus_tag="IWJ80_000095"
     CDS             94645..95025
                     /locus_tag="IWJ80_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9459867.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            95159..95314
                     /locus_tag="IWJ80_000096"
     CDS             95159..95314
                     /locus_tag="IWJ80_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP9459868.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            95383..95856
                     /locus_tag="IWJ80_000097"
     CDS             95383..95856
                     /locus_tag="IWJ80_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP9459869.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            95853..97547
                     /locus_tag="IWJ80_000098"
     CDS             95853..97547
                     /locus_tag="IWJ80_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9459870.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            97513..97698
                     /locus_tag="IWJ80_000099"
     CDS             97513..97698
                     /locus_tag="IWJ80_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9459871.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            97702..98877
                     /locus_tag="IWJ80_000100"
     CDS             97702..98877
                     /locus_tag="IWJ80_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9459872.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            98870..100393
                     /locus_tag="IWJ80_000101"
     CDS             98870..100393
                     /locus_tag="IWJ80_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9459873.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            100449..100733
                     /locus_tag="IWJ80_000102"
     CDS             100449..100733
                     /locus_tag="IWJ80_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP9459874.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            100720..101055
                     /locus_tag="IWJ80_000103"
     CDS             100720..101055
                     /locus_tag="IWJ80_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9459875.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            101350..101976
                     /locus_tag="IWJ80_000104"
     CDS             101350..101976
                     /locus_tag="IWJ80_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9459876.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            102718..103554
                     /locus_tag="IWJ80_000105"
     CDS             102718..103554
                     /locus_tag="IWJ80_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP9459877.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            103619..104053
                     /locus_tag="IWJ80_000106"
     CDS             103619..104053
                     /locus_tag="IWJ80_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9459878.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(104121..105443)
                     /locus_tag="IWJ80_000107"
     CDS             complement(104121..105443)
                     /locus_tag="IWJ80_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP9459879.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(105597..107237)
                     /locus_tag="IWJ80_000108"
     CDS             complement(105597..107237)
                     /locus_tag="IWJ80_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP9459880.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            107567..108037
                     /locus_tag="IWJ80_000109"
     CDS             107567..108037
                     /locus_tag="IWJ80_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP9459881.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            108666..109769
                     /locus_tag="IWJ80_000110"
     CDS             108666..109769
                     /locus_tag="IWJ80_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9459882.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTTPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            110053..110733
                     /locus_tag="IWJ80_000111"
     CDS             110053..110733
                     /locus_tag="IWJ80_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9459883.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            110823..111857
                     /locus_tag="IWJ80_000112"
     CDS             110823..111857
                     /locus_tag="IWJ80_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9459884.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            111871..112206
                     /locus_tag="IWJ80_000113"
     CDS             111871..112206
                     /locus_tag="IWJ80_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9459885.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            112190..114670
                     /locus_tag="IWJ80_000114"
     CDS             112190..114670
                     /locus_tag="IWJ80_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9459886.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            114726..116285
                     /locus_tag="IWJ80_000115"
     CDS             114726..116285
                     /locus_tag="IWJ80_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9459887.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            116282..117646
                     /locus_tag="IWJ80_000116"
     CDS             116282..117646
                     /locus_tag="IWJ80_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9459888.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLTRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            117817..118887
                     /locus_tag="IWJ80_000117"
     CDS             117817..118887
                     /locus_tag="IWJ80_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9459889.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            118902..119570
                     /locus_tag="IWJ80_000118"
     CDS             118902..119570
                     /locus_tag="IWJ80_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9459890.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            119590..120168
                     /locus_tag="IWJ80_000119"
     CDS             119590..120168
                     /locus_tag="IWJ80_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9459891.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            120571..121482
                     /locus_tag="IWJ80_000120"
     CDS             120571..121482
                     /locus_tag="IWJ80_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9459892.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(121738..122655)
                     /locus_tag="IWJ80_000121"
     CDS             complement(121738..122655)
                     /locus_tag="IWJ80_000121"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP9459893.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            122857..123294
                     /gene="zapA"
                     /locus_tag="IWJ80_000122"
     CDS             122857..123294
                     /gene="zapA"
                     /locus_tag="IWJ80_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP9459894.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            123352..123900
                     /locus_tag="IWJ80_000123"
     CDS             123352..123900
                     /locus_tag="IWJ80_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP9459895.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            124015..126375
                     /locus_tag="IWJ80_000124"
     CDS             124015..126375
                     /locus_tag="IWJ80_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9459896.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            126462..126776
                     /gene="trxA"
                     /locus_tag="IWJ80_000125"
     CDS             126462..126776
                     /gene="trxA"
                     /locus_tag="IWJ80_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP9459897.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            126958..128736
                     /gene="uvrC"
                     /locus_tag="IWJ80_000126"
     CDS             126958..128736
                     /gene="uvrC"
                     /locus_tag="IWJ80_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP9459898.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            128881..129780
                     /locus_tag="IWJ80_000127"
     CDS             128881..129780
                     /locus_tag="IWJ80_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9459899.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            129773..131035
                     /locus_tag="IWJ80_000128"
     CDS             129773..131035
                     /locus_tag="IWJ80_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9459900.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            131198..133096
                     /locus_tag="IWJ80_000129"
     CDS             131198..133096
                     /locus_tag="IWJ80_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP9459901.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(133395..134114)
                     /locus_tag="IWJ80_000130"
     CDS             complement(133395..134114)
                     /locus_tag="IWJ80_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP9459902.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            134451..134783
                     /locus_tag="IWJ80_000131"
     CDS             134451..134783
                     /locus_tag="IWJ80_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9459903.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            134770..136773
                     /locus_tag="IWJ80_000132"
     CDS             134770..136773
                     /locus_tag="IWJ80_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP9459904.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            136792..137262
                     /locus_tag="IWJ80_000133"
     CDS             136792..137262
                     /locus_tag="IWJ80_000133"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP9459905.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            137283..137867
                     /locus_tag="IWJ80_000134"
     CDS             137283..137867
                     /locus_tag="IWJ80_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP9459906.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            137882..138868
                     /locus_tag="IWJ80_000135"
     CDS             137882..138868
                     /locus_tag="IWJ80_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP9459907.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            138858..139169
                     /locus_tag="IWJ80_000136"
     CDS             138858..139169
                     /locus_tag="IWJ80_000136"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP9459908.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            139191..140972
                     /locus_tag="IWJ80_000137"
     CDS             139191..140972
                     /locus_tag="IWJ80_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP9459909.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            140965..142341
                     /locus_tag="IWJ80_000138"
     CDS             140965..142341
                     /locus_tag="IWJ80_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP9459910.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            142348..142983
                     /locus_tag="IWJ80_000139"
     CDS             142348..142983
                     /locus_tag="IWJ80_000139"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP9459911.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            143013..144368
                     /locus_tag="IWJ80_000140"
     CDS             143013..144368
                     /locus_tag="IWJ80_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP9459912.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(144560..145954)
                     /locus_tag="IWJ80_000141"
     CDS             complement(144560..145954)
                     /locus_tag="IWJ80_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9459913.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            146046..147017
                     /locus_tag="IWJ80_000142"
     CDS             146046..147017
                     /locus_tag="IWJ80_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP9459914.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            147014..147796
                     /locus_tag="IWJ80_000143"
     CDS             147014..147796
                     /locus_tag="IWJ80_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP9459915.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(147965..148336)
                     /locus_tag="IWJ80_000144"
     CDS             complement(147965..148336)
                     /locus_tag="IWJ80_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9459916.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            148470..148913
                     /locus_tag="IWJ80_000145"
     CDS             148470..148913
                     /locus_tag="IWJ80_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9459917.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(148956..149765)
                     /gene="yidA"
                     /locus_tag="IWJ80_000146"
     CDS             complement(148956..149765)
                     /gene="yidA"
                     /locus_tag="IWJ80_000146"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP9459918.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(149784..151151)
                     /locus_tag="IWJ80_000147"
     CDS             complement(149784..151151)
                     /locus_tag="IWJ80_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9459919.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(151135..151965)
                     /locus_tag="IWJ80_000148"
     CDS             complement(151135..151965)
                     /locus_tag="IWJ80_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP9459920.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            152128..152565
                     /locus_tag="IWJ80_000149"
     CDS             152128..152565
                     /locus_tag="IWJ80_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP9459921.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            152641..153261
                     /locus_tag="IWJ80_000150"
     CDS             152641..153261
                     /locus_tag="IWJ80_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP9459922.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            153450..153956
                     /locus_tag="IWJ80_000151"
     CDS             153450..153956
                     /locus_tag="IWJ80_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP9459923.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            153973..154872
                     /locus_tag="IWJ80_000152"
     CDS             153973..154872
                     /locus_tag="IWJ80_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP9459924.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            154959..155843
                     /locus_tag="IWJ80_000153"
     CDS             154959..155843
                     /locus_tag="IWJ80_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9459925.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(156035..156106)
                     /locus_tag="IWJ80_000154"
     tRNA            complement(156035..156106)
                     /locus_tag="IWJ80_000154"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(156071..156073),aa:Arg,seq:ccg)
     gene            156166..157500
                     /gene="rpoN"
                     /locus_tag="IWJ80_000155"
     CDS             156166..157500
                     /gene="rpoN"
                     /locus_tag="IWJ80_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP9459926.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            157703..158740
                     /locus_tag="IWJ80_000156"
     CDS             157703..158740
                     /locus_tag="IWJ80_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP9459927.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            158781..159782
                     /gene="gap"
                     /locus_tag="IWJ80_000157"
     CDS             158781..159782
                     /gene="gap"
                     /locus_tag="IWJ80_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9459928.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
BASE COUNT        50149 a        29550 c        30903 g        49275 t
ORIGIN      
        1 taaaaacatt ctaaattaaa tcagtaaaaa tttttattct aagattttta ttagaattac
       61 gcctaatttg tgtctctcag ggcaattcta agaacgtttc aagagatagc gacactatcg
      121 ttatcgaaaa aggcgaaaac ctatctcatt ttctgctaat gtaagttggt ttagcttttg
      181 ttaatatgac tttatgaaaa tggaggtaat taaatatgat ttttcctaaa gttaatgaag
      241 caagcttgaa taaaggatcc ttttttcata tctatgaaaa cgaagatttt gaactgtata
      301 atatcctttc acaaaaagag gtcatcgaaa accaaataat ctgccatcca attatttaca
      361 aatctatttt caagtatgca ttttttgctt ccaatgattt tcaaagaatg gagattaccg
      421 actgtatatt cgaaaactgt gatttttcag gctgtatatt gacaaaaagc atgttgcatc
      481 gcgtgaaatt tgtcaattgt aaattaacag gtactaaatt gatggaaagt tatattggga
      541 acacgttatt cgagaattgt aaaatggact atgtaaattt atctggttca aacataaaag
      601 aaagtacctt tgagcactct gtcttatcaa gcgcagattt tgtagactgt tccctcacta
      661 aaataatgtt ttctactaat gatttagaaa atgctagctt ttttgatact gatttaaaaa
      721 atatcgattt aagtagcaac tcctttgaaa tgattgaagt acgctcggaa catctaaaaa
      781 attgtacgct taatcagttg caagcacttg gttttgctag aaaattttta caaataaaag
      841 tagtataatc aaaatgaata tataagataa atttttagca atacaaatat ccataaaaaa
      901 acattgccta ttttaactct tcatgtatac tgactctatc ttcaaaaact gaatagtagg
      961 agttattatc atgaataaca ttttaggaaa aaaaatagaa cttaaaaaag ggatattggg
     1021 cactattgtc gttgtactcg gtttgttagg gttgctattt gcgccatcca ttattagcat
     1081 tttttcttta actatcactg gatttatagc atttacactt gtttttggta aaaaagatac
     1141 gaaaaaaatg ttctcgaaac ctgtcgctcc tgtcaaaaat atagccaaat atttttttat
     1201 caatgtagta atcagtgccg cagtctcggt atttttgcaa caaattttga aatggggttt
     1261 aacaggcaat ccaataaatg aggctttcag cccgttatta tttattattt tgcctatcat
     1321 gatacttgga gaagaattgt tctcagtgta ttttctcgct attttttcta gtaagttcag
     1381 cattcctgtc gctagtttct tgtcagcaat catctttgga tttatccatt acagtactta
     1441 tgataatgga aatattttac acactgtagc acacatcctt ttgatacaag gggtggcaag
     1501 attgttattc aaccaagctg ccattaaaag caactcgatc attacaagtt gggcagttca
     1561 tgtgatattt gatttcacag ctatattgct agtcgtttta ttctcctaaa tttttataga
     1621 aataatacat gctctattgc taagaaagca tactttattt cctttaaaca aaaagacaga
     1681 tgtatcaatg gattaaaaat ccttgatata tctgtcttct cttttaatgc cagctatagg
     1741 gatcgaacct acgacctacg cgttacgagt gcgttgctct accaactgag ctaagctggc
     1801 cttatccaaa agaaattata tactctattt ggctgattgt aaagagtccg atggaaaatt
     1861 agtttaattt catgatattt ttcataaaac gtggtataat tgatgtgaaa ggcaggtttt
     1921 atgaaaatgg caacttttgg aaaatttgat acaactatcg taccatcgga aattggtgaa
     1981 aaattcaaga tcaatactca cgtaatgttc acaattcacg gcaagtcgca aactggaacg
     2041 atttcaaagc aattgaaaaa ttccgctgtt gttgagattg atgaaactgc agaaaatgcg
     2101 aacttgatga cgcaaagtaa gggtgtagta atcgttaact acaaagaact aaaaaagatt
     2161 tgaatttgac catctctgca tttttgggat ggttttttta tatgcaaacg aaagctgtga
     2221 aaaagctgtt ctgcatcaaa cgttaaaaat gaccaaaaaa ggatgtgaca aaagttgtgt
     2281 cacacccttt ttcgtattta cttattttgt accaaacaaa cggtcacccg catcccctag
     2341 tccaggaacg atataaccat gttcattcaa tttttcatct agagaagctg tatagatgtc
     2401 aatatcagga tgcgcttctt gcaaagcttt tactccctca ggtgctgcta ccaaacaaac
     2461 aaatttaatg ttcgacgcgc cacgttcttt caatgcatcg attgccatga ttgctgatcc
     2521 gcctgttgcc aacattggat ctactacaaa caactgacgt gcatcaatgt cttctggaag
     2581 tttgacgaaa tattcatgtg gttccaatgt ttcttcatct cgataaagac cgatatgtcc
     2641 cactttagca gctggaatca attccaagat accgtcaacc attccaatac ctgcgcgcaa
     2701 gattggaata atcgctactt ttttaccaga caacgttttt tgtgttgttt cagtgatcgg
     2761 tgtttcgatc acgatgtctt ccaatggcat atcccgtgat acttcatatg ccatcaacat
     2821 agcgatttca tcgactactt cacgaaaaac ctttgttccg cagtttttgt cccgaataat
     2881 agttagtttg tgttgaatca atggatgatc gatcacttga aatttgccca ttttttaagc
     2941 tctcctttgt tctgtcgttt ctttttaatt gtaaaacaaa atcaagtgat tgactagtca
     3001 aactcgccat ttttcctaag aaaatccgtt tttgaaaaga agttttcctg cggacaagca
     3061 aagcttattc ttttttcttg atccaagaag tagataatgc cattgctgca cgcgttgcct
     3121 ttgtctgatc catagcattt accatgacaa aatcatggat gattccttgg aagcgtactt
     3181 gtgtaacagg aacccctgca tcacgaagtt tgcgcgcata agcctcacct tcatctctta
     3241 atacatccgc ttctcccgtt aaaatcattg ctgaaggaag atcatttagt tcatttatat
     3301 ttgctctcaa aggcgaagct gtgatttcag cacgctcttc ttcattcgtc gtatattggt
     3361 cccaaaacca catcattcct tctcttgtca aaaaatagtt ttctccgaat tcatgatagg
     3421 aatctgtatc aaaatcagcg tttgtgactg gataatacag caattgcttg gatattggga
     3481 ggccctttcg ttcttttgtt aagatcgtca tgactgtagc catgttacca cctactgaat
     3541 ctcctgcaac gattagattc tcgagatcca gattttttga cttggctaat ttattcagct
     3601 gttgcaacac agcataattt tgttcaattg ctgttggata ttttgcttct ggtgatcgac
     3661 tatattctgg aaagacaacg acggaattcg tacgaaccgc aagttctctg atcaatttat
     3721 catgggtttg agcactgcca aaaacccagc ctgctccatg aatataaaaa ataactggta
     3781 atttatccat attcccttct ggtcgtacga gacgaatatt gagttttccc cattcgccag
     3841 tatccaccat caagtcttca atatcgacgg aatatttgtt cacaggtgat tcttgtactt
     3901 cttctagtaa aactcttcct tcttccggtg ctagttcata gatacgcgga tgaggcgcat
     3961 ttgcatcact gaatgctact gcttcctttt ctaacgagat acgatcagtc atgacttttc
     4021 ctccctgaga tttagtgtga agactaaaat tacacctcat atactagaga gcatcaccga
     4081 tattgtcaaa gaatccgttt tcccaaaaaa taaacagaaa agcccaacat tgaacttttc
     4141 tgtttactaa tggaatttaa atttttattt tttatgcaac ggaaattttt cagttaattc
     4201 gcgtacacct gttttgactt cgtctagttg agcgttatca tcttttgctt gtaatgcttt
     4261 gacaactagt tcagccactt ttacagcatc ttcttctttg aacccgcgag tagtgattgc
     4321 tggcgtgccg attcgaatac cgcttgtttt gaatggactt agtgtttcaa aaggaatcga
     4381 gtttttattt acagtgatat tcacactgtc caaaatgctc tctgcttctt ttccatttat
     4441 acctaactcg cgaacatcga tcaacatcag atgattatct gtcgcccctg aaatgactct
     4501 ggttccaatt gcttgattga aaactttgac cattgctttt gcattggcga tgatttgttc
     4561 actatattct ttaaatgcag gatctaatgc ttctttgaat gctactgctt tacccgcaat
     4621 cacatgctct agagggccgc cttgaatacc aggaaatacc gcactattta ttttttttgc
     4681 taatgcttca tcgtttgtca aaatcatgcc gcctcgaggg ccacgcaatg ttttatgagt
     4741 ggtagtcgtt gtgatatctg cataaggaac tggattagga tgaagtcctg ctgctactaa
     4801 tcctgcgata tgtgccatat ctaccatcag ttttgcgcct acttcatctg cgatttctct
     4861 gaatttcgca aagtcgatcg ttcgtccgta agcactggca cctgctacga tcaattttgg
     4921 ttgatgtttt ctagctaaaa tacgtaccac attataatca ataacttctg ttgtcggatc
     4981 aacaccgtaa gcaacaaagt gataggtttt gccactgaag ttgactggtg atccatgtgt
     5041 tagatgtccg cctgcggaaa gatccattcc taagatcgtg tcccctggtt ctaccaaagc
     5101 gagataagca gccgtgttgg cttgagagcc ggagtgaggc tggacgttcg caaatttcgc
     5161 tccgaataat tcttttgcac ggtcaatcgc taaattttca acgatatcga caaattcaca
     5221 cccaccataa tagcgatgcc ctggataacc ttctgcgtat ttatttgtca agatacttcc
     5281 ttgtgcagcc atcactgctt cggagacaaa gttttcagag gcaatcagct ccaaattatg
     5341 ttcttgtctt tcttcttctt tggcgatcgc agcccaaaga tcagggtcaa acgttttgta
     5401 atctaccaca aacaacagct cctttttatt ttcctacaat tttatttttc ccatcaaaaa
     5461 caaattgcca aatattcgta tctcttctat tacgattagt tacgcttagt ttttcgttca
     5521 ctgatagaag atttacttga tagcattgta tcatactaaa agaatgcgac aataaccaat
     5581 tgatcatttt tcaaaataat tttgtcctgc tgcttttttc aagcgattca tgtaagcatt
     5641 ccccaatcca gtttctggat acacttgtac aaaaatcaaa tctaatccaa gtgttggttc
     5701 atctaatcct cttagacctg caaacaatcc tttagccgcc gcttctactg agttgtcgtt
     5761 atacatataa acagctgcag tatctgtccg cacttgatcc gcaatttcgg ggcttgctat
     5821 gacacctgca cggatcttct tattttttgc ccattgaact gcagttgacc aatcgccatc
     5881 tctgaccatc agaacacgtg tatcgggtga gtagtgtttg tatttcatcc caggggcttt
     5941 tggtgtttca ttttctttta caagatgctg atcaatagca acaggagatt cgatgactgc
     6001 ttcgatttgt tctttcgtta ctgcacctgg tcgtaaaatc ataggcatgg ctgttggatc
     6061 acttaaatca agaacagtgg attctacacc aattcgcgta gctccatcat caataatccc
     6121 ggtaattttt ccttgtagat catgatatac atgatctgca gtcgttggac ttggttttcc
     6181 cgacgtattt gcacttggac cgacaagcgg aacaccggaa agttcgatta cttctagtgt
     6241 ttttttgtta tccggtatac gaaatgcagc tgtggataat cctcccgtca caactgaggg
     6301 caaagtgtct tttttgatct taaatatcaa agttaatggt ccaggccaga agttcttcac
     6361 aatcttctct gctgccggat gaaagttgtc cacatactct tttacttgtt caaaagaggc
     6421 gacatggacg attaatggat tatcttgcgg tctccctttt acagaaaaca ctttcttgac
     6481 agcgtttggt aaaagagcgt tggcgcccaa tccgtatact gtttcggtag gaaaagcaac
     6541 aagttctcct tctttcagtg cttcggctgc ctcttgcaat tctgtttcat catatcgttt
     6601 cgtttccaca gtttatccac aacccctttt tcatacattc ttattttgtc tgagtttatc
     6661 ctcaagtttt ccactgtttg tggataatct atttgtcaat agtggataaa cttgttgata
     6721 aaccaaaaag tcgtttacat ccttgtcaaa taagtgtttt tttataataa aaaaacaaat
     6781 aaaaaatttt tcatccacaa cttcaaccat aaacttggat cattcgttca ttgccaaaaa
     6841 gatccttttt tatcgccact tttttatctg gaaatgcttg ctgaaatatt ttttttactg
     6901 cttcaccttg gcgaaatccg atttctaaaa aaatctttcc atttgatttt agtacgcttg
     6961 gtgcttcaac tgctattttt tcataaatag ctaatccatc gttctcagca aaaagggcca
     7021 ttttaggttc gaatgaccgg acactttcat ccatcagttc ccattcattc cgactgatgt
     7081 acggcggatt agaaataatc acatcaaatt gctgatccat tactggtcct aatgtatctc
     7141 catgataaaa actgattttc gttccttctt tctgagcatt ttctttcgct acttctaatg
     7201 cttcttctga aagatccaca gcagatatat gccaattttt tcttgctgct ttcaaactaa
     7261 tagcaattgc tccagtccct gtcccaatat ctatcacttc taacggtaca ccaggcgtct
     7321 cgtctaaaca ccattcgact aactcctctg tttctggtcg agggatcagt gttgcttcag
     7381 ttacctttaa ccgatgatca taaaaatctg catatcctag caaatactga ggtggatgat
     7441 ctgcaagcag aagtcgcaga tcggtttcga tcaaccgctg ttcttcttct gaaatctctt
     7501 cgttcatatg aaggagccaa tcaagttttt tccattgttt tcgttccaaa aataaaaatt
     7561 ggatactata tgcttctttc ccatgatcct ctaaaaaaga agaagcccgt gacaggactt
     7621 ctcgataggt cattttaccc attttgcata gcttccagtt ttgatgtttg atcatacatg
     7681 atcaatgcat caacgatctc atctaatttc cctgctaaaa tctgatctaa tttttggata
     7741 gttaatccga tacgatgatc agtcacacga ttttgcggga aattatacgt ccggatccgt
     7801 tctgaacgat ccccagttcc gactgctgat tttcgattgg catcatactc gctttgtgct
     7861 tcttgctgga tcttatcgta cacacgagca cgcaagattt tcattgcttt ttcacggttc
     7921 ttgatttgag aacgttcatc ttgcatagca acaacgatcc ctgttggtaa gtgagtcaaa
     7981 cgtacagcag atgctgtttt attgacgtgc tgtccgccag cacctgaagc atgatagata
     8041 tctgtacgga tatctttgtc ttcaatatcg atctctactt cttcagcttc tggcatcacg
     8101 acaactgttg cggtggaagt atggatacgc ccttgcgatt cagttgaagg aacacgctgg
     8161 acgcggtgcg caccactttc atattttaat ttcgaatata cgttgtctcc ggtgatcatc
     8221 atgatcactt ctttatatcc accgattccg gtaacgttag cttccatcac ttctgttttc
     8281 cagccttgtg cttcagcata cttttggtac atattgaaaa gatcgccagc gaataatgct
     8341 gcttcgtctc cgcctgctgc tccacggatt tccatgatga tatttttatc gtcatttgga
     8401 tcttttggta aaagcaagat cttgattttt tcttctaaga cttctttttc tttttttaat
     8461 tcagataatt cttcttttgc tagttcagcc atttcatcat ccagtttttc accaagcagt
     8521 tcttctgtat cggtgattcc ttgtactact tctttataac gacgataaac ttcgactgtt
     8581 tcgcgcgtgt ttgcttcttc ttttgaaagc tgcataaaac gctttgtatc agaaatcact
     8641 tctgggtcac ttaaaagttc tccaagctct tcataacggt cttcaattgc ttgtaattga
     8701 tcgtacatgc ttattctcct tctgtaatca ttgatgggtg aaagtaatgt tttcgacaaa
     8761 cggggtaata agcttcattt ccgccaattt gcacttgatc gccttcataa acagggtttc
     8821 catcaatata atgtaaattc atgattgctt ttttgtggca gaaccaacag attgttttca
     8881 tttcttcgat tttgtctgca taaagcaata aatattttga tccttcaaat aactcgtttc
     8941 gaaaatcatt tttcaaacca aatgccataa ccggaatatc caattcgtcc acaattctcg
     9001 taaaagcaat gacatgtttc ttttttaaga attgcgcttc atcgatcagc acacaatagg
     9061 gtttgctttc cattttctga attacgtcaa atacgtctgt ttcttcaaat atcggcatcg
     9121 cattacgttt cagtccgatt cggcttgaaa caacgcctaa accatctctt gtatcaatac
     9181 cgcttgtcat taagacaact ggcttatttt gttcttcata attatgtgcg acttttagga
     9241 tctcgatcgt cttgccgcta ttcattgcgc catacttaaa aaatagttgt gccatgctct
     9301 ttttcacctt ccagtttgtt cctatggtat tatacttgat tttcagcatt ttttacagtg
     9361 agaattttca tcaagcacag attagtatta aatctattgc tcacttttgt tccaattttt
     9421 tgacaagaac taaacatggg ttccattcat cccataaatt tggaaaaact tccaaccttg
     9481 aaaatcctaa tgattcgtag aaacaaatag tttgatcata tatagagtag tgcccctcgt
     9541 ctacagtctt cacttgcaaa tatttgtaat gttttgctgc gtaaccttct gcttctttaa
     9601 aaagttttgt tcctaatcct tggtgatgat agtctttttt tattcccata caatagaata
     9661 gatttcggca gtatcttcac ttgtctgtga aagagtgata aagcctaaat attcttcttt
     9721 tcttttttca caccataaag gtagtttact tgattcctcg atatattttt ccgttgattc
     9781 aggaagccca aaccactcag gcaagtctag taatatttct cggacacact ttcctttttc
     9841 ctctctatct tttacaaacg atatcggcat attctttcta gattctccct ttttgaattg
     9901 agtatagcat gctttctttc ttttcacgtg ctataataag ttaaaatttc aaagttttac
     9961 gtaaggagct atttgatgac aaaaaatatt tttgattcca tcgctaacca ttatgattcc
    10021 cctgaccgtc aagcattagc caagattatt cgcgaagaac taactactta tctaccgcgc
    10081 gacagccacc aaaaagtcct gctggattac ggaggcggaa ctgggcttgt tagtttaccg
    10141 cttgcggaaa gattcaaaga actgatcatt gccgatgctt cagaaactat gctgaaaatg
    10201 gcagaagaaa agatccaagc agcagatctg aaaaatgtcc gaacgatcca cgctgatgct
    10261 tctgtcgaat ttcctgctgt ccaagctaat ctcatccttc tatcacttgt acttttacac
    10321 attcctgata cggagaatat tcttacaaaa ctttatgaaa ttttagctcc tggcggtcaa
    10381 ttgatcatcg tagactttga caagaatgaa caaattagtc atccgaaagt ccataatggc
    10441 tttagccaag aagaattgaa taatcgattg gaaaaaacgg gctttgtctc tgctactagt
    10501 cataccttcc atcgtgggga aaagctattt atgaacaaaa acgcttcgct tttcttatcc
    10561 atcagccaaa aagcttaaaa tgagattgca aaaaaagtga gcaactccaa gaaataagaa
    10621 ggaattttcg gaaaaagaag ctgtgacaaa aaacatgtca cagcttcttt ttattttttc
    10681 atcacatgat tttaatttct gaaagaggcc aataacggaa aacaactttt ccttggatca
    10741 tatctttgtc gactaaccca aacattcggc tgtcttttga aatcaagcgg ttatcgccta
    10801 gaacaagata ctttcctttt ggcacagtat attcaaagtc actcgtaaag gtggatgaac
    10861 tttctgcttg tgcttggaat cctgagctat aagcatattc tccttgtaac ttatcttctg
    10921 aaaattcttt tttgaactca tccagataag gttcatcata ctttttgcca ttaatggtca
    10981 actggtcatt ttccatcttg actgtatctc caggcatacc gatgatccgt ttaacaatca
    11041 tccgttcttc atcccccggt tcctttgtag taataatatc cattcgttca tagtttgaga
    11101 tcttagatga aatcaatcgt tgtccatcac ttaatgtagg gtccatcgaa tgcccgttta
    11161 cagtaactgg tgtaaacacg tacactctag ctaataaaat agccgctaat acaaggatca
    11221 aaatccccca atttttcaaa aattctttca cagcttacct cctacttgta ttttcctcgt
    11281 tatttattac gttttttcac aatacttcgt tgtactttct ttattctaca atgtttataa
    11341 atgaaaggaa agaataagtg atgtgttcgt cttatctttc tcgttatata aaaaaggaac
    11401 gtgacaaaag acgtgtcaca tcccccttat tccgaataaa cgtttttatc gcaaccactg
    11461 atacacggcg tttcatcaca ttatgaatgt cttcttcttt ggctacgact ataaaccact
    11521 ggttcttcct gcgactgtgc cttttttgca atcaatttca gacgataagg ctcatgatag
    11581 cgtaacccgt ctttgacaaa atcaccgccg acgatgaaca atccgacagg ttccaatgtg
    11641 tcttctgttg agtcaagtcg aatggtccct actttttcca tgtccgagta gcttcgtcca
    11701 atatttagaa catactcttt ctctcctgtt ttcttgacat agtaataagg atacaatgta
    11761 tttctatcga ccagaaatcc atccatgcga ccgagttcat ttgttttgat ctcaccattt
    11821 ccaatcaaat accgcatatt ctcggctaat ttcaattgat aatcttcgtc ataagaaacc
    11881 atgacctctt gtgtattcac tttatcgatc gaatttccat tatctatcga ttcttctgtc
    11941 tggatgttga acgcttcatc aggtaaatac ttcacaaaaa gctgagtgac atcggctttc
    12001 gtgtttttat ctaaaaagta ataaaataca tttaaaacag taaaataaac aagcactaga
    12061 aatgcaagtc catataagaa aaattgaaga tagttttggt tttgccaaag tccataaaca
    12121 attctcaata aataaagcgt gggaatcaca cttaaaatcg tatatccacg gttaagatat
    12181 ttaggactga tgtctaaata gccaaggaat ttatgcaccc cgttgataag gtctagtaaa
    12241 aatgtcattg agaatcacct gcttgctggt tatacgtttg ggtatttcct gaatcttggt
    12301 tttgctgtgc tgtacgtgct gcatcatcat tttgcgtgtt atcatctaca gtcgtactgc
    12361 ttggtatcgt ttcggagcta gcacttgtat ccggacttgt acttggattg gttgaatccg
    12421 atggagtgcc atctggtacc gtattacttg tttcgcttga gctgtcagtt gtatatgtct
    12481 catcatacga cgaactagtt gttgtatcgt cccatgtact tgtcgtagtg gtttcattca
    12541 aactttccgt tgttgttggt tcagatgtcg agactgaagt agttggtgtt gtttcttcta
    12601 cagtactaga acttgaactt ggtgcacggt agctggaact attcacaata cctgtagtag
    12661 tcgcgacaat cgtcgagatt gttaatacag cgattggctt caaaataggt gctacttttc
    12721 gcatcttatc tcttccttca ttgaaattta ttcttgtttc cttactttac tacggacaaa
    12781 ttatacagtc taccttattt ctaccataaa ttctttattt ttttcttacg tttttccgaa
    12841 agatttcttt aattttcctc aaaaactacg gttgatgttt ttaagtaagc ttttgcttcc
    12901 cgattgattt ctttcagatc gcatccttgc tgcttggcag cgaacacgca accacaataa
    12961 cattggcgat aaatatcata ctctttgcac atttgcaaag agcgttcata gcctttgttt
    13021 ttcttgaaat cacttggcaa gtattgtgtc ccgtaaatct tttggatatc cattccgatc
    13081 tgattgatca aagctgcatt tttctttggc gagatggtca gggcacttcc aaagtaatcg
    13141 tatcctagtt cttgcgcttt ttgcgcaaca agatctaatc gcatattgaa gcatgcactg
    13201 cagcgagcac caccttcttt ttcgttttca agatggtttg ttaaaaccat tttggtgaat
    13261 tcatttggac ggtattcatc aacgattagc gcaacatgat ggtcagtttt ctcattaaat
    13321 tggtctacaa atcgtttttg ttcatgtaat cttcgcaaat actcactttt aggatgaata
    13381 ttcgaatttg aaaaaaataa cgtgatctct gcatattgag acatgaattc caatgtgtgc
    13441 gtactgcaag gagcacagca tgtatgtatt aatatcttag gctttacttc ttctttctcc
    13501 cattgcatga tcattttttt cagaactgta tcataattta ttttttgatt tttcattttt
    13561 tctacgattt gttgagcatt aatcaatttg ttattcctcc tccttctttt ttctaggatt
    13621 ttccttatta taagccattt ctattttaat cgaaagctgt attgttatct gtttgataat
    13681 ttctttgagt attcctaatt ccccttgtat actggtagaa acgactattg tcaggaggat
    13741 agtactgtgc atgttgattt attctttcga aggctgcagc ggcgaggaca gaagtttgct
    13801 gccaatcatt tctcatcgat ccaaatcatc gttttttatt atatattgat gacggtctta
    13861 tcactcgttc tattttatat gccgattttt cgcgaaccag atagccatgt ttcttttgtg
    13921 gacatgctgt tcatggcaat cagtacagtg agtgtgactg gactatcgac ttttgatatc
    13981 aattctgtgt tcaatgataa cggtattata ctgctggaaa tattatttca agttggtggt
    14041 ttggggatca tgatgatttc tactgctttt gtcatttttt ctaaacgacg gatcacactg
    14101 aaacagcgac agctgatcat gacggatatg aatcaaccgc gtttgtctgg tatcgtccga
    14161 ctgattcgga tcacttttgc tattttaatc tggtttcaat tactttttgg gacattcttt
    14221 tctatttatt tttattacag aggctatttt gatcgttggc gtgatgccgt tttttacggt
    14281 ttttatcagg cgatatcggc tgtcacgaac tctgggtttg acgtcactgg ggattccatc
    14341 aagccctttg cacatgacta ttttttcttg atcctgatca tgtttttgat ttttgtcgga
    14401 ggaattggtt ttcctgtatt gatggagtgc cgggaatggc tattatacaa acgttccaat
    14461 gcgaagcttc cgtttcgttt ttccttgttt accaaattag ctgtgttagc ttttgttatc
    14521 ttgtttgttt cgggaactgt cttgatttat ctattggaaa aggaccattt attccaagat
    14581 tccaatatga gtgtcaaatg gatcaattcg atgttttatt cgattacgac tcgtaatgct
    14641 ggtctgcaga ttcacgattt gggtgatttt caaatcacaa cactgatcat tttttcttta
    14701 ctgatgttta tcggatgcag tcctagttct gtaggcggcg ggattcgaac aactactgta
    14761 gcaatcatcg gtttgtatct atattccttc ctcaaaagtg aagataacat caatattttt
    14821 ggacggagaa tcgatcaaga tgatgtgcga aaatcagtcg ttgtctttat gctttctctt
    14881 ggtatgtgtt tcttttgtat cgttttttta tctgctacag aagaacaaac gctgatctcg
    14941 atcatcatcg aagtaacttc tgcgtttgga acaactggtt tatctttagg gattaccgga
    15001 gacttgtctg ttgtgggcaa aatcacgatt gctacactga tgtttattgg gcggatcgga
    15061 atgctatata cattgatgtt attcgtgcca aaagaaacac gagatctagg ctatgaatat
    15121 ccaagcgaga aaattattat cggttaaaat gggaatatag gtaagaggga ataataaaat
    15181 cacagcagct tgaaacaatc attttgtttc aagctgtttt tttgttaaat catgcgattt
    15241 tgtccgattt catgcacttt cagccgtaac cttttcttaa gaataagaaa agcaatttaa
    15301 cctttttttc aacatatggt atgattagtt gaatttattc gtcagcaaat cgtttttgga
    15361 ggttacataa tgggaatacg cagtcatttt gcgattgcag ttggaaaaac gtcgcaatgg
    15421 gcattaaaaa ctttttttaa aggcggatca agtttaccag gtaaactagc gctgaaagtc
    15481 gatccacata tactagatag tttggcaaaa gactaccaag tcgtcgttgt caccggaaca
    15541 aacggtaaaa cattgactac cgctttaaca gtcaatattt tgcgccagca attcgatgaa
    15601 gttctaacca accctactgg tgcgaatatg gtacagggaa ttgtatcgac tttcttgcag
    15661 gcaaaaccaa aaaaaggaca gaaaaagttt gctgtcctag aaatagatga agcaagtttg
    15721 agcaaagtca cagaatatat gaaaccagaa ctgttcttat ttacaaatat tttccgtgat
    15781 caaatggatc ggtatggaga aatctatacg acctataaaa tgattgtaga cggtgccgca
    15841 aagtcaccaa atgcaacgat tatcagtaac ggtgattcac caatcttcaa ttctatcgaa
    15901 acagtcaatc ctagaaaata ttatggcttt gaccatgaag aagatcgtga acaaatggct
    15961 cattacaata cagacggtgt cttatgtcca aattgtcatc acatcttgca ttataaaatg
    16021 atcacttatg caaacttagg gaaatattac tgtccaaatt gtgacttcaa acgtcctgaa
    16081 ttggattatc gattaactga tatggtacgg atggacaatg tgtcagcaga tttcgtgatc
    16141 gatggtcatg aatacggaat cgaagttggc ggtatgtata atgtctacaa tgctttagca
    16201 gctactgcag tggcagagta cttcaatgtc gatcctgata aaatcaaaca aggtttagga
    16261 tatgacgaga aagtattcgg ccgacaagaa gtcatcaata tcaatgggaa aaaatgtaca
    16321 ttagtacttg tcaaaaatcc tgtaggtatc aatcaagtaa tcgatatgat tggtctagct
    16381 ccttatccat tttctttagt ggctttatta aatgccaatt acgcagacgg aatcgatgtc
    16441 agctggattt gggatggtaa ttttgaaaac tttgccgata tggatatccc agctgttatt
    16501 tctggaggag accgtcatac agatatgact ttacgtctaa aagttgccgg gattccagaa
    16561 aataaattga cagaaacaaa agaattacca gaagtaatcg aaaaaatcaa agaattgcca
    16621 actgagcatg tctatatttt agctacttac actgctgtat tgcagcttcg taaagaacta
    16681 gctgctcaag gatttatcaa aggaggaatg aaccgtggct aattatgaac tcaatatcgc
    16741 ccacctttat ggaaatttga tgaacacata tggtgataac ggaaaccttt tgatgttgaa
    16801 atatatagca gaaaaaatgg gggtttcttg ccatacggag atcgttagta tacatgaacc
    16861 ttttgatgct gataaatatg acttggtttt ctttggcggt ggtcaagact ttgagcaatt
    16921 aatcatttca aaagacatcc agaaaaagaa agattctttg atcaactata tcgaaaatga
    16981 tggcgtcatg ttagccattt gtggtggcta ccaattgcta ggtcactact acatgggagc
    17041 taatggagaa aagatccaag gaatcagtgc actggatcac tacacgttaa gtcaggaaaa
    17101 taaccggttt atcggtgata tcgttatcca taacgaagaa ttcaatgaaa cttattatgg
    17161 atttgaaaac cacaatggtc ggactttctt aggtgaaggt gaacgacctt tggggaaagt
    17221 ggtacaaggt caaggaaata atggtgaaga ccaatctgaa ggtgtcattt accgaaatgt
    17281 cttcggttct tatttccatg gacctatcct tgcaagaaat gagaacctgg cagctcgttt
    17341 gatccgccta gctttagaac aacgttacgg taaagaattg gatctgccct atcttgaagg
    17401 acaaacagcc tagttattcg aaaaagggga atgtgacaaa aatttcgtca cattcccctt
    17461 tttcgtatcc tattttaagc gacttgcttg gcataacggt tcacgttttg cttgatattc
    17521 ccgattgttt tcggatcatt gattcctgtg acttcttgaa tcaattgttc caagttttca
    17581 tggatcttca tttcatgcaa ttgacggctt tgttcatctt caggatcata gtaattaaaa
    17641 ataatcccca ttgttgccgt caaatgtggg caagtcaagt tatgttcttg tagttctctc
    17701 actggacgtg taaatctttc ttgataacct aacttacgta atggtgtccg agcgacacgt
    17761 gtgatcgcat cagagatatt tttgttttgg aagcgatgaa tgattttttc aatatacgca
    17821 tgatgctgct ctgcatcaaa cccccatttg gcaatcagca aacttcctgt ttcctgcaaa
    17881 actgatttta attgtgccac aactaacgca tcttgcattg cttcatcaat ggtctggtaa
    17941 cctaacaagg cacctgtata agcgacagtc gcatgaccag tattgacact aaatagtttg
    18001 cgttcgatat atggttctag atcatctaca tataaaaccc cttttaattg gattttttta
    18061 ttttttcttt ggctatcgtc aatgacccat tctttgaaag gttccacttg gacgaataaa
    18121 ggatcttcat ggtgttgtaa tgggacgatt cgatcgactg ctgcatttgg aaagccgata
    18181 tatttgtcag taaatgatgt ttccggaaga tatttataca cttctttttc tagaaatgct
    18241 gaaccgccaa tcatattttc acaggcaatg atatctaaag atgttgtgct tccttccttt
    18301 tctctttctt ggataccttt tgcaatcaac tcagcaataa aaggtaagat attcgggcca
    18361 atggcggtag taactaaatc tgcttcagcg atttcttgga ttactttttc tggatttgtt
    18421 tgattgttga ttcctttgac attttttaca gtgatttttt cttttgattc atctgctaat
    18481 tcgatttcgt attcttttct ttcatttaaa gcatcaatga tcgtgtgatt cacatctacg
    18541 aaagtaatct caaatccatt agctgccaaa acctctccaa taaatcctcg accgatattt
    18601 cctgcaccaa aatgtactgc tctcattttt ctctctcctt aagcaattgc tagattttcc
    18661 gttacttctt ctttgctgag cgcgtctgct agctggacaa cattgtccat atcactgcaa
    18721 taaagtgcga tttgctgtac aagttgcaaa tgctcatctc cgactcctgc aataccgaac
    18781 aaaacagtcg caatcttttc ttcttcttcg gtaccaaaat ctacaccatc tggtacttga
    18841 acgacacaga tgccggattt tttcacatat ttttttgctt catctgttcc atgaggaatc
    18901 gcgataaaat tccccatata aacagataac attttgtctc tatctaccat ggcatcgata
    18961 tattcctcat caacgcaccc tgcttctaca agcatctgac cgcaatagcg gatcgcttct
    19021 tctttggttt taaaagatcg attgagctcg atcatgtcga atggcaaact ttgcatgctg
    19081 ttttcctccc ttaagctgtc tgtttcaaat attgtgcaat cgattcgtat ttttgcggat
    19141 tgaccatgct gttgactgat aaatgaatgg attgaggtgc agccatctgt actttttccg
    19201 ctaaactttc tttggctacg atcagtgaat caggttcatc ttttaaatct tggatattga
    19261 cttttcttaa cgggatagtt aaatggttct tgtctaatat atctttgagc acggtcatcc
    19321 ccattgttgc tgatccggaa cgatcatcgt ggacaagtag aatctctttg atttgtttga
    19381 tgtttgctga aagaaactct tctgctggct cattctcaat ttcagaaact gatgttttct
    19441 gcttgctgct taattcttga atgatttcat catatcgagg tgaattcaaa aagttttcta
    19501 ctgagacaaa acgcgcgctc ggtgcttttt gttttgctct ttcttgtagt tcgacctgag
    19561 taacgatcaa tgtattcgag tcatctgtca gttggctgat tgcttgattg gtcaccgttt
    19621 gaggcaagtc tgcttcctgt actttttttc tcaagatcga agcgcccatc gcacttgatc
    19681 ccattcctgc atcacaagca aaaatgattt tttgtacgcc agagaatgta gctccttgag
    19741 gaataccttg ggcacctttg gaagcttgct tagctgcctt tgtctcttga acagattttt
    19801 cgaagtcttc atttgtatct ttcgcatccg ctttcaaaat tactgcagaa ataccaaacg
    19861 aaacagctgc ggcaacaatc acgcccaaaa taacaggaag gtaagaattg aacggtgtca
    19921 tcgctagaat cgcaatgatc gaccctggag aagctggcgc tcttagtcca gcatccatca
    19981 attggaaaat gaaacttcct gacattcctc cgcctataac tgataagaat aataacggtt
    20041 tcatcataac gtaaggaaaa tagatttcat ggatcccgcc taaaaagtga atgatcatgg
    20101 ctcctggtgc tgaagatttt gctgctcctt tgccaaacaa agtaaaagca agtaatacgc
    20161 caagtcctgg accaggattt gcttcaagca agaatagaat agattttcca gcctctgcta
    20221 cttgttcagt tcccaatggt gtcaaaatcc catgattgat tgcattattc aaaaataaga
    20281 tttttgctgg ttcaataaaa atacttgtca atggaatcaa atgggcagat aaaatgatct
    20341 ctacaccttt tgccattgct tgagtcaatg catcgacaac tggaccaatc gcagaaaaac
    20401 ctaataacgc taatccaaaa ccgataagac ctgctgaaaa attattgact agcatttcaa
    20461 aaccggattt gatccgtttt tggaatagct gatcaaattt cttgatggca tatccaccaa
    20521 gaggtcccat gatcatcgcg cctagcatca ttggaatatc tgttccgaca atcacaccca
    20581 tcgttgcaat tgccccaaca actgatccgc ggtcaccagc aattactttt ccgccagtat
    20641 aaccgatcaa taaaggaatc agataagtta gcattgggct taccatggta gcgaaagcct
    20701 catttggtaa ataaccatct gggataaata gagccgttaa tacaccccag gcaatcagtg
    20761 ctccaatatt tggcatgacc atgcttgata aagtactgcc taatttttgt accctcgctt
    20821 ttagcgaaat gttgtttttg ggaatcgtcc gttccatttt gtctctccct ctctcctttt
    20881 gatactctaa ttgtatccgc ttactaaaaa gagaaatagt cgtactttgg cacgaaactt
    20941 tgtgccaaag gtttgagtat gtcaacaaaa taaaaagatt tcggtatcag aaaacttcaa
    21001 ttttaaataa aactgttatt ttaatttttt tataatatgc aaacaataac ttttgttagc
    21061 atttaattta gatgttttcc attctattat gttattaaat tttatattgg aggcctatct
    21121 atgacattac tgatcaaaaa catgaacatg aaacgtggta aaaaacgtgt gattcgacaa
    21181 ttgaatcttt tagtagaatc aggagaagta gctgctttga ttgccccgaa cggatttggc
    21241 aaaacaactt tgctgaatgg cattgcccaa ctgctgccaa caacttatga tcaattgtcc
    21301 cttcacaaca tctctcctac tgcttataca gattatcgcc ggtctttctt ttttctagaa
    21361 tcaacgaatc agttatccga ctatctatcg gctgttgatt atttgacttt gatcaaatac
    21421 tattggcaga gcaaagtaga atgccaaact gttttccaac gattgaagat gactgagtac
    21481 cttactttgc cgattggaaa aatgtctcta ggaatgaaac aacatgtctt gcttgctgct
    21541 tacttgatca gtgattcatc tgtcatgtta ttcgatgaac cgttgaacgg attagaccca
    21601 ggaagtattg atatcttgaa tagcctcatc agacaatggc agaaagaggg gaagacgatt
    21661 cttctttctt cccacgctat cgcgaatatc caatcgattt gttctaaagc atgttttcta
    21721 aaaaatggag aaatcgtcac agaatcctca aatatggaag agattttagc tatttatcgt
    21781 aaattatatg aggtgtaact atgtctttac ttacatttga acgcaatctc ttactaaaaa
    21841 acaaagtaaa ctttttattt cctctattgt tggtcgtgct ctttgcattc ccactttttt
    21901 ttgatcataa acttgcttat acagaattcg atgaactgaa tcataactat gaagagatgc
    21961 aacgtttgat tgaaacgttg aaagaagatg agaatgagaa agaatttgtg gaaagtttag
    22021 agaaaagcaa caaactgatc gaagcaatct tacatgcaaa aaacacaggg aatgtccagc
    22081 aaatggtcga agcaacttat cattatgaaa aagatatact ggatcgactg atttctggtc
    22141 aaagacaagg aattccaatc attgaacaac aaaaacgtgt cgagttgctc cgttatatga
    22201 aagaacatca gatccaaaga tattctattt ttgacttgcc agcgcatctc tctttagcaa
    22261 attactacga aaatattttt tcaggaatga tttctagttt tttgatttta tgtataaccg
    22321 ccttgtttct ttcttccatc atctcttatg aaaaaagaaa gcaggtaatc tctctagtca
    22381 atctattacc ggatagtatg gtaaagaaac atagcatccg ttttaccatt tattatggtg
    22441 cagctatgct aagtctcgtg atgccatttt tgatcgtctc tatactagta ataatcaaaa
    22501 atggtttggg agacttccgt tatcctgtag gaacgatcat tggtcaggag atacgaatcc
    22561 tgccgatgta tgagtatctt ttccagagct ttctgttcct gttgttgtgg gtgctctttt
    22621 taagtacaat cagttttctt ctatctgctt tatttgaaca ttctctagtc aatcttttag
    22681 gtacacttct ttgtcttttc ttagcagagt atcgcttgtt ttcttcgatt ggatggatag
    22741 aatcaatcag tcattacttg ccaacttctt atgttgattt tcaaaatgtg atcataggag
    22801 gagatatttt ctcgccacta gcttcagaac aggttacttt catgaatggt atcttgacac
    22861 ttggtatatg gagtatcgtt ttattattca taggtatggg cactatctat ataaaaaaga
    22921 gctattaaaa cacaaaaata ataggggcgg tgacaaaaac cttgtcaccg tcccctattt
    22981 caaataaacg gttttctaga ataaatagtt ttcaaaagct tgcatccgca gtaataagaa
    23041 cctcccaaca aaaatagctc ctcttatttt tatcttggtt gaagttatta cttgatgcaa
    23101 ggcagctttt tttactgtga acattggtta tatatgtcca tgttcaaaaa tcatttggac
    23161 ttcttcaatg gtttccgcat cactgagcgc cattacttgg tcgatgtctg aacagaaaaa
    23221 ggctaaatct tggagcaccg ctaactgatg ttcttttttt aaagccacta caaataagag
    23281 tgtcgcgatt tttggttcct cttttgttcc aaagtccaca ccatcaggca cttggactaa
    23341 atagattcct tcttctacta cttcttcatc tttgtcagtc ccatgtggca aggctacgaa
    23401 attcccgata tagacactcg ctttttcttg tctttcgatc attgattgga tatatgccgg
    23461 tttgattccc ccttgttggg tcatccactg gccgatctcg gtcaacgctt ctttccaatt
    23521 ttgataggat tgatcgagca atattttttc tgctggtagc tgtatcatct tttcaccttc
    23581 atttctttat ttcttcaatc aggattttcg ctaacaactg ataaacgatt tctttatttc
    23641 ctgagcggaa gatctcggtg ttcaagtcat tcatgatgat tgctccactg atctttccta
    23701 aaagaatccc gtctttttct tggattggcg aaggcgccag cataactaac atacgggaca
    23761 aatcgactgg ctgtctatcc atcccttcaa tggtcaacgg atgtttcaaa ttgaaaatac
    23821 aaaagatcgg tttagaaatc aatgagctgg atgtatggaa taaccccata tttgtatgag
    23881 gaataccgat aggtgcttgt tcatatcgct taagcagccg gcgatgaacc acctcttcat
    23941 ccgttactag tccttcagga aaataaggaa ataagctgcg aatcgtttcg gaaatcgtct
    24001 caggattatc cagttcttgg ataaaaaaac gaccgagtaa ttgattgatt tgatccatga
    24061 aatgcattgt ttcctcataa gtatcttcta tcatccactc gttattgctg ttatacgtaa
    24121 ttttttgcct ttttttcttc tcaatatgcg aaaaagcttc ttttagctgc ttgacttcat
    24181 cttctagcag tagcggggag actaaaatat attttccacc gtatcctggt aaaatagatg
    24241 tagaaataac aacatcatat tcttcttgta aatcgatttt ttttaaatca gcaatccgat
    24301 agaaatgaat ttgttggatg aagggaaaat accttttcaa tcgtagttcc aacatgctag
    24361 tagaagctaa tccacttggc gaaaatcctg caatactgat attgctttct ttcggacttc
    24421 gttccaatga attcgcaaaa tgaaggacca tataagcgat ctcttcttcg gataattttt
    24481 tttcttgaaa aatccgctct actgctttac ggattgcatt cgcaacttcc tcgtattgaa
    24541 ccatgatttt ttctaaaatc ggattggtca gtgtctcttc ttgtaaaacg gctcttgaaa
    24601 acacaccaga taaatgagtc aacagcattt tgtacaaagt ttcatcttca taaaaatgga
    24661 cattcgtctc ttcacttact tcttgaatca gttgttttac taaataagca agctgtgtat
    24721 cgaacgtctc ttcaaagaaa ttctgatcaa atgagttcgt gaagtcattt aataatcctg
    24781 caaaaaagac gatctcattt actggataaa gttgttttgt ctcagaagcg atccgtgaaa
    24841 acaatcgttt agctgtctgt agtaattctt tgtttgccag actattatat gtctcatttg
    24901 tcagtaaatg atttttcgtc acacgctcca tcgtcaatgt cagcatcaaa accaaatgtt
    24961 caagctttct atcacttaga ttggtctttt tttggatcaa ttcttccccg attactttgc
    25021 gtgcaagaga tagatgttgc ggatcaacga atcgaaaaaa ataattcagt gatgcatcga
    25081 aagaaatact atgaaaaagt tcatattcat tgatttctaa ttctaataca ttcgctgtca
    25141 gaagccggcg gtatttttcc ggtccttcga tctcgtatcc ctggttccga atgatttcca
    25201 aaggcagttg gcgaatactt tcttccaact gtttgatatc tgcataaaaa gtggtattac
    25261 tgatgcgata tgattccatg aaatgagcaa gactcaacgg ctctgtcgtc agtaacaact
    25321 cggctaaaat cgcgtgttgt cgtttagcag tggataagtc attacttttc ccatctgtat
    25381 tttcttttaa ttgctctaat acttcttctg atgcctcaat gacaaatgag cctctagctg
    25441 cttttttcaa agaagcattt accgatttca atgagacttc aagattatct aattcgcggt
    25501 aaatagtccg tttactgacc ttcaataaat tcatcatttt ttggatagat acaggtgacg
    25561 ggctactgag aaattctgct aataattgtt tttctctggg cgataaatac atcttatatt
    25621 tctcccttca cgagtttttc gtattcattc gtagctaccc aatgttcaac tagaactagc
    25681 cgagggatct tttcttctag ctgttcagtt acagctagtt ctctttgtgc gatataaatc
    25741 gtttcttctt ggattttgat tttttctatt gattcttttg agataacaaa ctgttttttc
    25801 gctttttttg ctaattgact taaaatcgtc atgcccatag tttgtgagcc gcgtctgttg
    25861 tttttataca aaaatacaat attagcaggc gacgtttttt gttctgtgat atctgctatc
    25921 tctttttttt cttcccgctt tggaaattgg atcgcttctt ttattcgtga cagcaagata
    25981 tctgtttcca aaaagtgatc cagtacatag atttgacttt gaggtacttt ttctcttgca
    26041 agacccgcta gttcatgttg cacgataacc agctgattta cttggttatt caattcatag
    26101 atagagctgt atccgacaga aagatcgatt ttttctgctg ctaatttctt tttcagcagg
    26161 gcggctccca tagcacttga tcccatacct gagtcgcaag caaaaatgac ggaatgaatg
    26221 atagatggtt ccaagatttc tcccctttct ttgaccgttt tgttctcttt ttctttattt
    26281 ggtaatttca atagtatcgc tgcacaaatg aaggtcacaa atgtactgat tgctatccct
    26341 atacttaccc cagctagttg tggataaggc gcattggcaa aaatcacgag tagtgaccca
    26401 ggcgatacag gtgcagctaa tcccgtatct aaccattgaa atatcaacgt gccgcttgcc
    26461 ccaccagcca ttacagctaa aaatagatac ggtcgcagca agacaaatgg aaaatagatt
    26521 tcgtgaatcc cgccaatcgc atggatcatc aatgctccac tcgcatttac tttttcctgt
    26581 ttttctgcaa aacaaaggat agaaagtaag atccctaaac caggtccagg atttgcctcc
    26641 atcaaaaata aggaagagta cccgactgtt tgtgagaatt cgattcctaa aggtgtcaat
    26701 acaccatgat tgattgcatt attgaaaaat agtactttta gaggttctaa gatcagactg
    26761 acaagtggta acagattcat tgtgactagc caagacacaa cggaggcgag caacaagtga
    26821 acactattta aaactggttc aataaaaacg attccaatta tacaaataac gctaccagtc
    26881 agtcctacta gtaggttgct gacaagcatt tcatatccag ccttgaattt cacatggaat
    26941 ctagaaacaa attgttcata tacgatgccg gcaagcggcc cgataatcat cgctcctacg
    27001 atttgtggag cattggaacg agcgatcaac cctaaagaag caattgcacc ggcgactgcc
    27061 cctttttgtc cccctaccga tttcccgcct gaataactga tcaagatggg taaaagcagc
    27121 tggatcatcc aatggcatac ctcttctaat gtggtgtgca aatcgccaga cacacctttt
    27181 gttgctaatg acaagattcc ccaagcgata aacagactta gatttggcat gatcatttca
    27241 tttaagtgat gtccagcttt tctcagttgt ttcatagaca cgcgactgct gttgtttgct
    27301 ttccatttgt attccatctt tttcaccaga cttctattta ttgatggacg gattttatgt
    27361 agaaaaagca gcaaacgaaa aaccgtctgc tgctttcagc tatctgggaa ctatttgcct
    27421 ggttcagacg caatgattgc tagttcacct tggaccgccc actcagaaac gccatttgga
    27481 ataatacagc ttgttccttt ttctaatgga tatgtttttc cgtcaacgac aaggtctcct
    27541 gctccttcga ttaccgtcat caatgtataa ggggcttgtt ttttgaaaga tgtgattcct
    27601 ttaatatccc actcatatac attgaaaaac tcagtttcta aataagtgac gatcgatgaa
    27661 ttcccttttc ggacttcttt gatttgtaat tctggtgttt tcgcagggac agttgttaca
    27721 tcgatcgatt gttggatatg cagctctctt gtttgtcctt gcgcatcttt tctgtcgtag
    27781 tcatacacgc ggtaagtcgt gtcagaactt tgctgtgttt ctaaaatcat gattccttta
    27841 ccaatagcat ggatcgttcc gcttggtaca taaaagaaat cacctttttt aactggtact
    27901 ttcttcaaaa ggtcatccca gcgcccatct ttgatcatct cagctagttc ttctcttgtc
    27961 tttgcatgat gaccatagat gatttctgca ccaggttcag catcgatgat ataccagcat
    28021 tcagtttttc ctaattcacc ttcatgtttc atcccatagg catcatctgg atgtacttgt
    28081 acagatagat catcttctgc gtctaagatc ttgatcaata atggaaacac tggttctgaa
    28141 ggatggccaa acaattcctt gtgttctgac catagttcat ctaatctttt ccctttgaat
    28201 tcgccatttt ctactacact gacaccatga ggatgcgcac tgatcgccca atcttctccg
    28261 attttatcgt tagggatatc aaaaccaaag actgaacgta aacgacttcc tccccaaatt
    28321 ttttcttgaa aaactggttt taaaaacata ggttcctgca taattcaaac tcctctcatt
    28381 tttcatagct attatagcat tatttcggta ataccgcttt ctctttcaaa aagttttcaa
    28441 caatttcatc tcgacatatg agatactccg cacgatgatc gacaggatgt acgcgatttg
    28501 acaaaaatat caacgcttct tgtgttttta tatcaatcaa gaaaaacgta cctgtataac
    28561 cagtatgaaa aagaatcgca tgaccgtctt ctacatcctt ttttaagtcc catcctaaag
    28621 aacgatagcc attttttttc ggtgtttgat cttgcagtaa agaatcaatc gtctcttctt
    28681 ctaaaaaacg accattacgg atttttccct tgttcaaata catttggaca aaagtaatgc
    28741 tgtctttcaa atcaataaag agacccgcgt tacctgcatg ttcagccaaa actcttgctt
    28801 tcggatcatg agtttgtcca cgcaaaacct cacccgaagg cagttgttct gtgggaacga
    28861 tttttttgga aggaaaagga agaaataagc tgttcatcat tcctaatggt tccaatactt
    28921 gttcttgaaa gatttccact gcagtttctt ggtaaatttc ctctagcatc aacccaagaa
    28981 gaatcgtacc ggcatctgta tattttactt cttttcctag ttgctcacct ggttctagct
    29041 tcaaataagc ggcgatcaat tcttcctgag atagcttatc tctgtttggt atccatgttt
    29101 tgatatcaga agtatgtgtc agtaaatgac gcaaagtgat tgtctgattt tcaaattcag
    29161 gaagatattt atgaagcggt tgatcgagtt ctatctttcc atcttctaat agacgtaaaa
    29221 ctaatgaagt ggttgcaacg actttcgtca gtgaggctgc atcaaacaac agatcttctg
    29281 tcatcagctg cttttcagga acgacttggg caaagccggc tgtaaatgtt tctacttgat
    29341 tttcttttat gaaggcgact gctacacctg gataaaatcc ttcttccatc tttttcaaaa
    29401 taagctgttt cgtgtgtgga tacatctttt tctcctgctt ttctatcaga ttcttacagt
    29461 ccaagcttaa agcttaatct atgaggaatc aacctttttt acacaaaaaa actgcgcgaa
    29521 aaaccgcgca gtttcccccg gtatactatt ttctctttct atctctagaa taagcgatag
    29581 tatgaccgtt cataaaatga cgagacatgc gacaatcgtt aagaaagaaa aagttaagcg
    29641 tcgcatcgaa tgtcagtttt attcttttct tttggctcac tgacgaacca ccattcgatt
    29701 cctatagcat catagattgt gagtactgtg tgctttttgt ctaagaagaa atcaactgat
    29761 agttgctgca agtgagcaat cagtccttcc atgctttcag tatcgatcat gaatttcaga
    29821 aaatcaagtc caatcacttc atctgtaggc aaatcaattg cgcctccgct agcttctgtc
    29881 aaaccaacat ggaaatctcc ttcattcaat acatggatgc cctcttcttc attatgtacc
    29941 ttcaaaccaa gtacatgatt aagaaattgg cgttcttttg ttaagttagt aacatttaaa
    30001 tggatttttt caaaataaga acctgttgat agtttactga atttttcttc agattttctt
    30061 aataatgctt cttgatccat tgtttctgga atttctcttt ggtcgttctt attctcttca
    30121 tggtaaattt ccaattcatt ctcttctggg tctacgagca aaatccccat acgtccatga
    30181 tcttctaaag cttctttgat tggataattc attttacgga tacgacaaag gatatctgcc
    30241 atttcttctg tgctagggat aatcaatgaa aaacggttca gctttttgat cacccccatg
    30301 tgatcattcg ctctaggact ttcttccagc agcaacattt cacttccagc ttcttctgtt
    30361 cctaaaatgg ctaattcgtt ttcttctctt ttcaaatcaa agccaacaac gttcttgtaa
    30421 aaatcaatca ttttatctcg gtctttcacc cgaatggcta cactttttaa ataggtggca
    30481 gcccccaatt gaaaatcttt catatttctt gctcccctct acgtcaagct ttattttaac
    30541 gatggattct tcttctttca agcaataact ttgattttgt taaaaaaaat gtgagaaaat
    30601 tcgcattttg cttgtttttt gtaaattaat gagtaaaaac tcttataaaa agaggttgtg
    30661 aaaaagctgt tctgcatcaa gtaataagaa cagccaaaca aaaatagctc ctcctatttt
    30721 tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta aggaagaggt
    30781 tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg gctttctaca
    30841 tttttgcata aatttggctt aataccgaga aactggcttt tgaacaccgt ttaccaagag
    30901 gttgtgagcc tgccgtttaa ctccgagtca caaggagcaa tttcaaaaaa cagcttctca
    30961 ggtttgttga ctattgtgac ttgtgaccga ggagttggca ggagaacacc gtttaaggaa
    31021 gaggttgtaa aatcagcggt ttgacctgag tattagagat acaaaacgga aaaatagctt
    31081 ctcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac accgtttatt
    31141 cggaaaaagg gactgtgaca cgacttttgt cacagcccct aaaaaaaata ataaatttgt
    31201 actattctat gaagcttctt cttcagaaaa ctgactgtta taaagatctg catagaatcc
    31261 gtctttttcc atcaatgtat cgtggtttcc tgtttctacg atcgaaccat gattcatcac
    31321 aataatgttg tctgcatcgc gtattgtaga caagcggtga gcaacaacga aacttgtccg
    31381 gttttccaat aggcggttca tcgctttttg aatcagtatt tctgttcgtg tatctacact
    31441 agatgttgct tcatcaagga tcaggacatc tggatttgct aagaatgccc gcgcaattgt
    31501 gattagctga cgctgccctt gcgaaatatt gcttgcatcc tcgtttaata cagtgttata
    31561 tccttctggc aatttacgaa caaaatcgtc tacgtgagca gctttagcag cctcaatgat
    31621 ttgttcgtct gttgcatcgt cgttcccata tttgatattg tcatagatac ttcctgtaaa
    31681 cagccaagta tcttgcagta ccattgaaaa ctgtgcgcgc aattcatcac gtgaaagatc
    31741 acgggtatct acaccatcgt aacgaatact acctccagaa acatcgtaga accgttcaag
    31801 aaggttgatc agtgtcgtct tccctgcacc agtcggacct acaatagcga ccatctcacc
    31861 aggtttgaca tccaagttaa aatcagtcat tagcaagtta tctggtgtgt agccgaattg
    31921 aacattttca aaacgaactt tgtatggtgt atctttttct tctggaaggc cagatttttc
    31981 attggacatc tcttcttcat ccaacacttc aaatacccgt tcggctgaag caactgttga
    32041 ttggatagtg ttcatcaaac ttgcaagttg agtgattggt tgagagaact gtgttgtata
    32101 ttgcaagaac gcttgaacat cccctaatgt catgttccca tttgcaactt ttactccgcc
    32161 tagtacagcg acgaacacat agcctagatt tttaacgaaa ttcatcaacg gcatgatgat
    32221 tgccgaaata aattgagctt tccaaccagc ggcatacaga ttctgatttt ctttttcaaa
    32281 tacttcctgg tctttttcag tatgattgaa tgttttaaca atggtatgac caccatatgt
    32341 ttcttctact tggttattca ataaacctaa acttttttgc tgatctgcga aaaacacttg
    32401 tgatttcggc gctacgatca tcacgatgat caagcttaaa gggatcatca ataacgcgac
    32461 taacgtcaat tgccagctga ttgaaagcat catcacgatg accccaatca atgtgactgc
    32521 actagttaca aattgagtca aattttgctg cagcgtgctt gcgatgttat ccatatcatt
    32581 gattgctcga gacatgatat ccccatttgt atgtgtatca taataggaaa tcggtacacg
    32641 attcattttg gcttccaaat catgacgtaa ttcataaact gttcgttggg aaacacgtgt
    32701 cataataaat tgctgtaaaa agttgaatac agcagaaatc aaatacatca cgattactgt
    32761 cgcaatgatc tgcgcgattt tatcaaagtc tatcggaaat tttgttactt gttggccttg
    32821 ttgcatctgt tgagcgccag ccattacacc tttgaatatt tctgtggttg ctttccccaa
    32881 gacctttggt gtttggattt ggaagatcac ggctgctatt gctagtacaa atacagcgat
    32941 gatcgcaacc atacgtttag acatatagcc aaacaatcgg cgtaccgttc cccagaaatt
    33001 tttaggcttt tcacctttta caccgagatt tctgccaggt ccaccatgtc ctggaccgcc
    33061 tgctggtcca tgagagggac gtgtgtttgt tttactcatg ctaagtcctc ctctcttagt
    33121 tgtgaatgaa cgatttcttg ataagtttcg tttgttgcta atagttcttc atgtgttcct
    33181 ttgccaacca gttttccttc atctagcaca aggatcatat ctgcatccat tactgtactt
    33241 actcgttgag caactaaaac ggtgatactt tctttcatat tcgttttcaa tgcttggcga
    33301 agattggcat ccgttttgaa atccaacgct gagaaggaat catcaaacac atagatatct
    33361 gctgttttga ctaatgcccg tgcaattgcc agacgctgtc tttgtccacc agagaagttt
    33421 cctcctcctt gttccacatg gctatctaag ccatcttcca tctctgaaac aaaatcttta
    33481 gcttgtgcga tttctaatgc tttccaaatc atttcatctg ttgcatccgg cttaccatac
    33541 tgcatattgt ctcggatcgt tccagagaac agaactgctt tttgaggaac aaagcccatt
    33601 aattctcgca aattatattg agtcatgtca ctaatgtccg ttccatttat ttggatcgaa
    33661 ccagattcga tgtcatataa tcgagggatc aaattgacta aggtcgtttt acctgaacca
    33721 gtccccccta tgattgcgac gatctcgccc gacttggctt ggaaatcaat atcttccaat
    33781 gcaagttttt ctgctccatg atatcgataa ttgacatgat tgaaggccaa tgttgcgttt
    33841 tttccagcaa aagaaactgt tttaggattt tcagggtcat ggataccgat tttttcatcc
    33901 aacacttcat taatacggtc agctgaagct tgtgcacgag gcaccatgat aaaaatagcg
    33961 gaaagcatca taaagctgat caagatctgc atcgcataag tcataaatgc aatcagatta
    34021 ccgacttcca aagtcatatc tgctatgtag tgtccaccaa accaagtgat tgctacgttt
    34081 gtaccgctca tgatcaatgt catcattggc atcatcaatg cgacgatcgt attaactttg
    34141 attgccgtat ttgtataatc tttattcgct aaatcaaaac gattttcttc aaagcgtgtc
    34201 ttatcgaatg ctcgaatcac tctgacccct gtcaatcctt ctcgaaatac caggtttaaa
    34261 cggtcagttt tcttttgcat acttttgaat aatggaacag caaagtacat gattccgccg
    34321 atcaaaataa tcattactgg gatgacaact aagaagattt ttgtcaactg atgatctttt
    34381 tgataagcaa gtatgctcgc gccaatcaac gtgattggtg cattgatcat catacgtaaa
    34441 aacatttgtg taaccatttg gatttgagtc acatcattgg tcgttcttgt gatcaaagag
    34501 gccgttccat atttatcaaa cgcatctttt gtcagactct ctgatttttt atagacatcc
    34561 gtacgcaatt gcttacccag tttctgtgat tcacgagtag caaaaaatgt atttgcgata
    34621 gcagctaaga tactgatcaa agaaaagccg atcatcacaa aacctgtatg ccaaatataa
    34681 tcgacatctc cttgcgctac ccctttatca ataatattgg aggtaagtgt cggcaaatat
    34741 aaatctgcca gtacttggat caccatgaaa actgcagctg caattgctga agtcaaggag
    34801 atccgtttta cgatttttag catctagttc ccttctttct atttatatac tccgtttttc
    34861 agccaagaaa gtttcaaatt gaattcactg attgcttgct ctactgagta atcttcagat
    34921 gttgaaaata agcgatacga atgcgcaatt gttgtaaata cagtattcat cactaattgg
    34981 atcagtaatt ccaattcatg atcatttttt actttcagct tcgtccgatc gatcaattca
    35041 agcaactctt ttgcatcttg tttgtgttct ttatgtggat cgtgaggcgt tttttggcga
    35101 ctcgtatgag gagcgacttt tcttgaggca cggtaatcca tgttcataaa caagttacga
    35161 taaaaagaag cattctcgcc ttccaaaatc tcataaatca ttttagaaaa ataggattcg
    35221 aatcctttga atagatcacc atcggattct ttcattagtt gtaacatatc tttagagctg
    35281 tcacgtctca ttgtctcaaa gtaataaaaa taaagatcct ctttatcttc aaaatactga
    35341 tagaaactgc ctctagggat atccgctaat ttgacgatat tagcaatcga agcttctttt
    35401 aagggtactc tagaaaactc gattctcgca gcctcgatta gtcttttttg tttttcttta
    35461 ggcaaatgga aaaatgtctg tgtcggcata tggattctcc tttctttcaa gatgacacat
    35521 tgtcatatga cgctatgtca ttataacacc tcctacctgt tttgcaagac atttgcatag
    35581 tttttttcat tatcatccga tacattaaaa aagtgataag aggaatcctt ccatttgaat
    35641 taatgagagt tgcccattaa aatagccaca tactaaacaa aggatgagat aaatggccca
    35701 atcaattagt gtccaagact tcaataaatt gcctctcgat gctgacagca tcgttttaga
    35761 tacaagaaat accttagatt acacaaaaaa ccacttaagc cactcgcttt ctatccccgc
    35821 tgctatcttg ccaagacaat taaaaaatct agatccaaag aaactttatt atattctaag
    35881 ctatacaggc aggcgatcag aagcattagc cgatcaactt actgctaatg gttttcgtgc
    35941 tgtttatatc attggtggga tggaagctct tcagtcgagt gttgcttgaa tattgcttgt
    36001 tagattcaaa agaggctgtg acaaaaaatc gtgtcacagc ctcttttata ccctgtcttt
    36061 ttttagttat ttattcttca accttatcaa tatgacttga tagccttttc tttttcgagt
    36121 aaactgattt tgttagtcat agttataaca atcatagcaa taaaaggaga aggtaagcat
    36181 atggcatctg ttttagtagt caaaggtcat cccctaactg cagaagaatc gcgcacagtt
    36241 aaggcattga cgtctttttt aacaagttat aaagaaaatc atccagatga tgaagtgact
    36301 gttttggaac tttatcgcga tgatattccg gaaatcgatg aagaattatt atcaggttgg
    36361 gaagcattac gtgctggtgc agaattcact tccttatcag aaagtcaaca acaaaaaatt
    36421 gctcgtttca atgaattgac tgatcagttc cttgctgctg ataaaatcgt tatcgcaaac
    36481 gccttatgga atctaaatat cccaacaaaa ttaaaagctt ggttcgatac cgtcaatgta
    36541 gctggaaaaa cgttccgcta tacagaaaac gggccagaag gattagtgac aggtaaaaaa
    36601 gcactgcaca tccaatctaa tggcggtgta tacaacggac aagactttgc ctctcaatat
    36661 gtaaaaggta ttttaaactt tgtaggcatc gatcaagtag atcagctatt catcgaagga
    36721 atcgattatg accctgaccg tgcagatgag ttgatgcaaa atgcattaga taaagctgct
    36781 gctttaggta aatcatttta aaaattgaga taaatgcata caaaagagac agaggaaaaa
    36841 atcttcaaag cagcatttgt gccttggata ttttcccatc tgtctctttt tgttattctt
    36901 tctgcttttt caacggattt tcttcataaa gagaagagat tgttgtgtac cattcaaaaa
    36961 catgagtaat cactacttta gctgctgcat aaacaggaat ccccaagatc acaccgatga
    37021 ttccgaacaa tttcccagaa gtcagcagaa ctaataaaat cgttactgga tggattgcta
    37081 actgattccc tagtactaat ggagagatca agcgtccttc tattgtctgc tctactgcaa
    37141 aaacgatcag tactttgatg atcatgaatg gacctgctac aatggctaaa aagatagccg
    37201 gaatcatcgc taaaaatgat cctaagtaag ggatcaggtt taaaaaaccc gcaataatcc
    37261 ccaacgtgac agcatactct aaaccaatga ccgcaaaacc aatcataaac atgattgcca
    37321 cagcaaaagc cacagttagt tgtcctctta tataagaaga aacttgacta ttcatttcac
    37381 tcagcacatt caacgtcggt ttttgcatgc gtgtcggtaa aaatttgaca aaataaggtg
    37441 ctaattgttt accgtctttc aatagataaa ataagataaa cggcatcgtc agcagagcca
    37501 ctaaaatagt cgctaccgcg ccgacaaaac ttccaagact ctgaactgta ctcttagata
    37561 tatcttggat catatcgcct gccgaattca tgaacttatc acctgctgtt tccaactgtt
    37621 cgcgaaattg gttgaacagc gggtcccgca aaatctcggt taatttatta tcaatcgtat
    37681 ctacatattg agggaagtta ctgatgaagc tcactgtttg ttcctgaatc ttagggatga
    37741 tcactacgct cccccatgcg attagtgcaa caaccaaaac aaacagccca atgatactgt
    37801 aaagccttgg tacttttcgc ttttcaaagt aatcaactac cggattcatc aagtaataca
    37861 agattcctgc taaaatgata ggcagaccta cgattgctaa aaattgccag attggtgcaa
    37921 acaaatatga aacttttgta aataaaaaga cgatcagcaa tattaacaat acaacaagta
    37981 aggctgtgac tacttgatta tttaaaaacc agcgccaaaa ccacgatacc ggtctgttct
    38041 tcttattttt tccttccata attcctcctg ttagttataa aaaatttggc ttcccaaacc
    38101 aatagcaggc agtctatgcg gatgaagata gattccattt gcaatttgtc cttcttctgg
    38161 acaagcatca aaaatcaatg atacgtgagc tactgtcttt aaattcccat tggccatcgg
    38221 accgacataa tcgatcgtat atacctgatc atcaaaagca attgttccgc ctgctttcaa
    38281 agaaaaaggt tcgtcttttg atacttcttg aatcaccgaa tattttcgta aggcagctgt
    38341 tgctgtttta tcgaacaaaa tcagcatggg ttcttccttg tttattgctt ctgtcccaat
    38401 ttctgtaatc tttgcctgaa tcattgcact ctctccttta attgctttca aaaacagtat
    38461 aacacattct cattctttag ccgtgtggaa taattatgtg atagaataaa aacgaaaact
    38521 atttcattac aattaggagg aatcagctca tatgatcgaa aagataattt taggaaccta
    38581 tacgcgtcgc gaaagtaaag gaatctatac gatcgacctt gatacagaaa aagaagaatt
    38641 agccaacctt tcattagctg ttgaagaaaa tagccctacc tatgtagcaa aaagtaaagc
    38701 tggcaatctt tacacagtta ctagtgtgga tggattaggt ggtgccggtg cgtacgataa
    38761 ggattttcat tttttgaatg cggtaacaga gtctggcgct cctttgtgtt atgtagcggt
    38821 tgatgaagca cgtcaattac tatacggggc gaactatcat aaaggtgaag taaatgtcta
    38881 ccatatatta ggagatggcg aactgaaagc agcagattca atattccatc aagaagcgac
    38941 aggtcctcat aaaaaccaag atcatgcgca tgtccactat acggatctga ctcctgacca
    39001 acgattagtc gtatgtgatc ttggtacaga tcgtgtgtat acttatgatg tgtccgaaaa
    39061 cggcaaattg aatcttgtaa ctaccttcac tgcagagcca ggaacaggcc ctcgccatct
    39121 tgtctttcat ccaaatggac aattcgctta tttatttggt gaattagaca gcacagtccg
    39181 cgtgctttct tataataaag aaacaggtgc ttttgaagaa ttacagaaaa tatcgacctt
    39241 gccagaagaa ttcgacggtg aaaatggagg ggcagctatc cgcatttcaa atgatggcaa
    39301 gttcctttat gcttctaacc gaggacataa ctcgattgct gtattcgcag tttttgaaga
    39361 tggttcccac attcaaaaca agcaaatcat ctcaacagaa ggggattttc cccgcgattt
    39421 cgcactagat ccaagcaata attttgtcgt gtgtgccaat cagaacaccg ataatctgac
    39481 attatatcgc cgtcatcctg aaacaggatt actggaatta ctgaaaaaag atgtctatgt
    39541 accagaatgt gtctgtgttt attttgatcg ttagtaagaa aaggagaatg aaatgaatca
    39601 gcaagacgta ttagacagac tacgggaaga actaaatatt cctttctttg atgggaaaat
    39661 cgaagataaa gagtatagcg aagaggaata tcagaaactc aaagccgatt tacagaacta
    39721 ctttgaacaa tatgtacaga atgtagaaaa ctaatcagct ttccatacta tctgaaaaga
    39781 acatcaggac aagacttttc cgtctatcct gatgttcttt tctacaaata aaaaaccgcc
    39841 gagatggtta gactcgacgg aaaaggagta tttactcgat tttgagtaag tacaaggttg
    39901 ttgttggtta taggtatgtc attatcataa aggattttga tcactctatc ataaaggtaa
    39961 tattacattt atattagata actctagttt ttattgttat caaagatttc ttttattttt
    40021 tcaacaacaa attagtcatt ttttggatgg cagctgtcaa ttggtctgtc gcttggtctt
    40081 gttttgcttc agatgtattg aatagatctt catctacccc tgattcaaga tcgatacttt
    40141 ggatcatttc ttcacagcct gctacatatg aaagataagc tttttcaaaa tttttatgaa
    40201 tgcccattgc ttttgctggt ggacgcaaag tactgacttt cataagcatt gcccgatatt
    40261 tttttgttcc ttcttcaaat aacaggatga tctcttcaag agtcgatttg tccagttcac
    40321 ttgtttttcc ttcatcaatt gcgtttctga tcacttcata tttcggatgc attccttctc
    40381 cgattgcttc tgtttccttg acgatttgat cgatggactg cacataatag ccaatattcg
    40441 gtaacatgtt cgttttccta ctttctccat tgttgttatt atttattcta cttgttttta
    40501 tcctcaaatg acaagtactt gaaaaaattt aatggtttct atacagctcg ccattcttat
    40561 tcgtgctcaa agaaaccatc tgctaaacta tattgtttcc ctgcgacaat gtcatattgg
    40621 atcaattgat attcgtcgta aatctcttgt tgcgagcgat tgaattgcgc ttctttatac
    40681 cactgtccgt tttgatagta cagttctcgt tcaaacgccg gaagttcctg ttctaatgat
    40741 aaaagcagct gataaaagcc atttgttgtc tgattcgtct gttccattag attcgctgca
    40801 aaataaaacg gactggtgat tgcatcgtga gtcgtttgct tttctaattc tcctttactg
    40861 tcgaacataa gaaattgtgt ttcatgcaat gtttgtttgt cattttttgc ttggatcgta
    40921 tctgaataga tccctggaag atgatccccc caaaaaacaa ctaaagtcct tctagaaaga
    40981 ttcttcaatt cttccgtgaa ctgcttcagt gccgtgctgc tataggaaat atcttgcaga
    41041 taattctcta agtcaagtgt cccacttcca tctgaagttt ttgcaggata gtctagatta
    41101 tcatactttc catcgtaggg catatgtgtc tgcatcgtga ctaaatgaat gaactgaggt
    41161 gttttttctt ccttgagaag tgtcattact tctttgtaag ctgactcatc agaaatatat
    41221 ggattattct ctatcgtatc cgtataggtc atcgtacgct cgctgatgaa ctggtcaaat
    41281 cctaatgtct gataaacatc ctctctttta tacatcgaag tattgtatgg atggatcgct
    41341 gtcgtctggt atgattgtgc gtttaacgct gaaacgatag aaggaagctg atccattttt
    41401 ggtacaagca tcgtgtaagg ggtggtgagt tgcgcattaa ataacgccat agaaaaacca
    41461 gtcaatgctt caaattcgat atttgctgtt ccaccgccat agttttgaga aagcatattg
    41521 ccactatatg tttgatcagc tacttcataa taatctgcca atggctcacc agatacttct
    41581 acgccgttta atcgactagg atctgagaaa ctttcactca tcacgaacac gatgttcggc
    41641 gactcttcct ctacagtttt atttttttca tcggctagct tctggtattt ttcagtgatt
    41701 tctttgatct tttctttcga atatccttca ggttcatcca ttggttctac cttcagattg
    41761 tacaaaaacc caccgataaa accagtgtta tagtagttca ttttctggct gtaaggtatc
    41821 catagtgccg ttttattata tgcttttcgc aacaaattat cgggattgtt gaaattactg
    41881 atatacccta gcagtccaat tgccacaagc agtgtcgtta atcggatcaa ttggaatcgc
    41941 cttccttttt tcaggcttcg aaacatgtac cagcaaaaca atcctatcac ggttcctgcg
    42001 gcagctaaga tcactgtaaa cagcatagtg ccggtcatat ctttcagcaa tccgatttca
    42061 gtgatcatct tcaaatcgtc tggataaatc ggttcttgcc gataactcat tttcatataa
    42121 tttgcaaaac ctaatacacc gatggctacg acatacgtaa gggaacctaa agggacagat
    42181 cctgctagtg caatcagaaa aataagaata atcagcaaga cgagacaggc taagaagaat
    42241 ttttccgtat gccaagaaaa agcaaatttc aacgcaaggt ctacggatag atcattttga
    42301 caccactgca aaaataaatt actaaaaaca acaagaaaaa agatactgat gattgctgct
    42361 gttcctttta aaatcttttt ctgtttggtc gaaagtctgc ttatatatct tttcaaatgc
    42421 ttgctccttc atttctatca ataaaagaat gttgcttctc ttattttagt tgtacgaaat
    42481 tcgaaagtaa agaacggaaa gaagaattaa tgaaatccaa atattctctt tctttatggc
    42541 ctttctggtt tctttaataa gcggaccttt cgtactccag aaattttttc tagctgttgt
    42601 ttcacttgat tgagctgttc aaaatcagta ctgtctatgt caatcaatat gtagacatgc
    42661 tcattcttat ggttacgcga tatatctgcg atcgctagct cgttctcatt cagcagagaa
    42721 aatatctctg ctaatatatt ggttttcttt tgataaaata tagtgaatcg aaacggcgct
    42781 tggaaaaaca ttcgtgcgga tggataattg accgcttctc taaccgtacc gaacaaaaga
    42841 taatctttta aggcttcaac tgccaaacgt ccgctgtctg ccaaagcttc ctgagtcgat
    42901 ccccctagat gaggcaacat aagtatacgg tcattttcta agaactcttc ttctggaaaa
    42961 tcagtcaagt atttagccag atgttgtttt tccaatgctt gcataacagc atatttatca
    43021 acaatttcac ttcttcctac attgataagt acagcatctt ttttcatttt ttctatattc
    43081 gcctgatcga tcaatccttt tgtatcttct gtcaacggca gcatgactac gatgtaatcc
    43141 gaggtagaaa gaactgtttc taaatctgct tgccgaacat aatccaactg ggcatcgcgg
    43201 atagaataac ccaagacatc catccctaag ctatagcaac ttagcgctac ttttgtgcct
    43261 atcgctccaa gaccaagaag accgatggtt ttgccttgca attctcgtcc cacataagca
    43321 cttcttttgt tttctgcctg ttctaaaata ttaggacctg ttagtgtttg taccatacga
    43381 cttgcttcga ttatcggacg ggaagataag agcaaacagc acaaaacaag ctcttttact
    43441 gcattagcat ttacacctgg tgtattcatg acgatcgtcc cgttttcaga agctttttca
    43501 acgttgatcg tgttcacccc aacaccagct cttgaaattg ctagcaaatc tggtgtaagc
    43561 cattcatctt tcactttcgt actacgaata actattcctt cggctggcaa atcatctatt
    43621 attttgaaat attccccttg taacttttct atctcttgtt ctggaatagc atcaatcaaa
    43681 tgaacgtgat acataaaacc ccctccttac taacaagtat ctcgaaattt ctgcaattag
    43741 acaactgttt accatttgac ttcctagaag caaaaagagc agttagagca gagtaggctg
    43801 agtgataaaa ataaggctat gacaagttac tgccataagc cttattttcg agagcaaaat
    43861 attgagggtg agatatcttg ataaatggtg ttccgttagc tgacattgat aataagacta
    43921 aatttcaaaa aagttgaaaa gcaaatttcg tcaatctctt cttatttctt aaaactaagt
    43981 ggctttttca caacctcttt ttatctaata accactgttt ccaactcaac taattttgcc
    44041 cacgtttcaa tttgatctgt tgttagattt agcgagacaa cggtatgatg accgccacct
    44101 gcctgaatcc atgaatggac accttcatgg aaattcggtt tagttttcca taacacacgt
    44161 gcaacaggta aattaggtgc ttcagtcgtt ggttcaaagg cttctacttc attgatcagc
    44221 agtttataat gcgtaccgaa atctgccatc gatacgacta ccccttctcc tgctttccca
    44281 tcaaatacaa gacgcgccgg atcttctcta tctcccattg ataaaggaga aacaacgatt
    44341 cttggtttcg tcgcagcaag cgtcggatct acttccatca tatgggattc caagattgct
    44401 tcttggccac tagctagctc gtaagtataa tcttccatga atcctgtatc tttccctcga
    44461 gccatgattt tcagcaaccg atcaatcgct gcagttttcc agtcgccttc cccagcaaaa
    44521 ccgtatcctt ctgccatcaa acgttgtaca gctagtccag gtagttgttt cattccatac
    44581 aggtcttcaa aattagtcgt aaaagcgtta tagcctcctt cttctaagaa acggcgaata
    44641 ccgatttcct gctgagcttg tacttttaca tgttcctccc atgtcttttc ttcataatca
    44701 ccatagtcga aggtatacaa ttccttgtac tcttcaaata attgatcgac ttcttcgtct
    44761 gatacgcgat cgattacttg tacaagatct ccgataccaa aataatcaac agtccaacca
    44821 aattgaatct gtgcttccac tttgtctcct tctgtgacac ccacgtttct catgttgtca
    44881 ccaaaacgag ccacttttat tccaaaactt tcattatagg ctactgcaac atccatccaa
    44941 tcagcaatct ctttttggat ctcaggtcgt ttccagtagc caacgacgat tttattttgt
    45001 ttatttaatc gagcattgat gaacccatat tcgcggtcac catgagcgga ttgatttaaa
    45061 ttcatgaaat ccatatcgat cgttttccaa ggaatgcttt cgttatattg cgtagctaaa
    45121 tgaagcaatg gtttttgtag aagctttgtc ccgcggatcc acatttttgc tggtgagaaa
    45181 gtatgcatcc aagtgatcac acctgctact tcttctcgat aattgacctc tttcatgatt
    45241 tttgtgatcg tatcagcagt aactgctaat tcttgaaggc ggattggata aggcaattgg
    45301 ccattttcat tcagttcatc gaccatttct tgtgcatgtt tctttacttc tcgcagtgct
    45361 tcttccccat ataaatgctg tgatcctaca acaaaccaaa attctttttc tccaatattc
    45421 aacataatta gccactcctt ttatttgttt tgtccgtaat acgcattttt tccgtgtttt
    45481 cgtaaataat gtttatctaa aatccgttga ggcagctctt ctgaaaatga attgagctgt
    45541 ctggtaaata aattcatctt gcatacttca tctagtacga ctgcgttcat taccgcactt
    45601 tgcgcatctt tcccccaagt aaatggaccg tgtccgtgta aaagtacccc tggaattgct
    45661 aagggatcaa ttttgcgttc tttgaatgtt tctatgataa cttttcctgt ttcatattcg
    45721 tacccgctgt cgatttcctg ttgtgtcaaa aatcgagcac aaggtaccga tccataaaaa
    45781 gtatctgcat gcgtcgtccc catcgcagga acatccaatc ctgcttgtgc ccagatcgtc
    45841 gcccatgtcg agtgagtatg gacgattcct ccgatttgag gaaatgaacg atataaaaca
    45901 gcatgagtag gcgtatccga agagggattg agtttacctt caatgatatg attgtccaag
    45961 tccacaacga ccatgtcgtc tgctgtcatc tcttcataac tgactccgct aggtttgata
    46021 acgaaatatc gtgtttcagg atcaaaagcg ctcacatttc cccatgtata tttgacgagt
    46081 ccgtgtttag gcaagtcaag atttgcttgg tatacttctt ctttcagttg ttccagcatt
    46141 ttctatcact cctttttctt gcaattgatg aatcgctgtt ttttcaatgt cgatcccctc
    46201 acggtaacgt ctcataaata tctgatagcc tgctatttct tctgctgaag gtgtgatggt
    46261 gattccttgg tcttgtccaa acacgtcttc tgccaagaag ttttctaaag acttcccatg
    46321 tcgatccatc atataggctg ctaataacgc gattccccac gctccacctt ctccggctgt
    46381 gtccatcact gtaactggtg cctccatagc agatgctaga atccgctgtc caacttctgg
    46441 tgttttaaaa atccccccat gccccactaa acgatcaatt tggacattct cagatttcaa
    46501 tatatccatt ccgattctca ttgcaccaaa agcactggct aaatggatcc gcatgaaatt
    46561 cgctaaatca aaacggctac ctggtgtacg tacaaataat ggccttcctt catttactcc
    46621 tgtaatgttt tcaccggaaa aataaccata agaaagtaat cccccgcaat ccgtatcgcc
    46681 ttctagcgct ttgttgaata ataccgtgaa taatttatct ctgcttatct ccatacccaa
    46741 agaattggta aattcttcaa aaatactgac ccaagcattg atatccgaag agcagttatt
    46801 cgtatgaacc atgcctacta gttctcctga aggtgtagtg accatgtcga tttccggatg
    46861 gacattttct agttcttttt ctaatacgat catagcgaag gcggaagtac cagccgaaat
    46921 gttccctgtc cctttggcta cactgttcgt agcgaccata ccggttcctg catccccttc
    46981 tggcggacaa accgggattc ctggtctcaa atttcccgta gggtcgatca gataagcccc
    47041 tgattcgctt aacttgccag cttcttctcc tgctactaga actttaggca aaatgtgatc
    47101 caagtcaatc gctacgtgcg ctttttcgat taactgacca aaaatatttt tcatttcttg
    47161 atggtaggtt ttcgttgaag gatcgatagg aaacatgcct gatgcatctc ctacgcctag
    47221 tactttctca ccagtaagct gccaatggac ataacctgca agcgttgtca aaaaattaat
    47281 ttctttcaca tgttcttctt tattcaaaat agattgatac aaatgagcga tactccatct
    47341 ctgcggaata ttgtattgaa atttttccgt taatattcgt tctgcttctt cagtgattgt
    47401 atttcgccaa gtacgaaatg gtactagtaa ctgttccttg ctatcaaatg ctagataacc
    47461 gtgcatcatt gcactgaacc caatcgaccc gattgtcgta agattctcgc catagatttc
    47521 gaatacttct gctgccattt ttcggtaact gacgcgcagt cctttccaaa tatcttctaa
    47581 tgaataggtc cagataccat tttctagttt gttttcccac tcataactac cagcggcaat
    47641 aggagtaaaa gatgaatcaa tcaatacagc tttgatccta gttgatccaa actcaatccc
    47701 taaagatgta cgaccatcta tgacatctaa catactttgc gcatttactt ccttcataat
    47761 gatttaactc cctccttctc gtcaaacctg atcaaaccgt ctttttagta gcgcttacaa
    47821 tgattattgt atacttttgt acgtacatgt caagcagtta tttacaagaa tacaataaaa
    47881 aacacttgtt ttgtacgtac ataagttata aaatgaaatt gcttacaaaa tctgatcaac
    47941 cgccttctcg aaattctaga aggagagaat acttatgaaa aaagaaaaaa aagtttcctc
    48001 atcgtttatt tatttttttg gcgcatttgg cggcattcta tttggatatg atattggtgt
    48061 tatgaccggc gccttacctt ttttacagca tgattggggt ttggcaggta aagcttcttt
    48121 gataggatgg atcacgtctt cggtcatgtt aggtgcgatc cttggcggct ctttatctgg
    48181 tcttctttct gataagcttg gacgacgaaa aatgattctt ctttctgcac ttatttttat
    48241 ggctggttct gtcctttctg catctgcccc tcacaatggt tcttattttc tgattgcagc
    48301 aaggattctt ctaggtttag ctgttggcgc tgcatctgcc ctagttcctg cttatatgtc
    48361 tgaaatggct cctgctagac taaggggaag attgtcaggg atcaatcaag tgatgattgc
    48421 ttctggcatg ctcctttcct atgttgcaga ttatttgcta aaagggttgc ctgaaacgat
    48481 ggcttggcgc gtgatgttag ggctcgctgc tgtccccgcg ctgattttat ttttcggtgt
    48541 tttagctcta ccagagtcac cgcgttttct tatgcaatct ggccgcttgg aagaggcaaa
    48601 aagagtattg aactatattc ggacaccaaa agaagctgaa caggaattcg aacaaatcca
    48661 gctgaatgtc aaacaagaaa agacaaccgg aacttcttgg catactttgt ttttagaaaa
    48721 atatcgttcc ttagtcattg ccggtattgg tgtagcagta ttccagcaat ttcaaggagc
    48781 taatgctatt ttttactata ttccgctgat cgtagaaaaa gcgactgggc atgcagcgag
    48841 cgatgcattg atgtggccga tcatccaagg aatcatttta gttgctggtt ctttgctctt
    48901 tttggttatt gcagacaagt tcaatcggcg aactttattg aaaataggcg gtagcgtgat
    48961 gggactttct ttcatcctac cagctgtttt aggaactgta ctggatgctc atacaaattc
    49021 gttgttgatt ctcttgtttt tatgtattta tgtcgcattt tattcttgca catgggctcc
    49081 actgacatgg gtaatcgtag gcgaaatatt cccactagct gtccgcgggc gagcatctgg
    49141 cctagcctct tcttttaatt ggattggttc tttcctagtt ggcttgcttt ttcctgtgat
    49201 gactgcttct atgtctcaag aaatcgtttt cggcattttt gggatcatct gctttttagg
    49261 ggttttattt attcaagaga tcgtaccaga aactcgtggg aaatctttag aggaaatcga
    49321 acaaagtgca tctaaaaaaa cttatcctaa acgtatttct atccatcatt cttaaagagg
    49381 ttgtgacaga agtggtcagc ttcaagaaat aagaaggaac cgcttagttt ccagtcacaa
    49441 ggaacaattt caaaaaacag cttctcatgt ttgatgaata ttgtgacttg tgaccagttg
    49501 gtaggagaac accgtttagt ggagaggtgg tgaaagaaac gttcagcctt gagcgatgac
    49561 aaaagctctc acaaacattg cgacgagtaa ccgtaggagc aataagaggc ggtaaaaacc
    49621 cgtctcttat ttttcgtttt ctttcactgt ttcccgttca actagtacag gttcatagag
    49681 aatatttggc tgtgcctgtc ctttttcaat cgttgtgacg atccaccgtg cggcatcttg
    49741 tcccaacttt tcttttggat gcgtcaatgt tgtcaatttt ttattcccta aatggcttaa
    49801 agaagaatcg tctgttccta cgatcgatag gtcatctgga atatgatagc cattttctgt
    49861 caacttatct agcattaaac tagctatttg atcgttatag caaatgatgc catctaaagc
    49921 ttgttcttct ttcaaccgtt gtactgtttt ttcagcgatt gtttctcgtg tttccgtggt
    49981 gaaagtaatg atatcttccg tcaaaaaacg cacattatac tgttcacacg cgctgataaa
    50041 acctttcatc ctgtattttc cttgaagatc gtcgattttt gtgatgaaca acagacgctg
    50101 atgtcctttt tttatcaaat attctgctgc cataaaaccg actttcacat catctaaaca
    50161 aatataagag gcatttaact cttcataagc agcattgatc atcaccatcg gaatgccttg
    50221 ttctctcaaa gcaacataaa gtgctaagtt tgggttatac tgattacttt tcgtaggctc
    50281 aacgatcaaa ccatctaccc cataagcaat cattttcttc aaacactctt tctccaattc
    50341 gtgatcgtta ttcgtactgg ctaaaagcaa tgaataccca gattctcgta attcattttc
    50401 gattcctcgg ataatcgacg gaaaaatata atcagagaga tatgtggtga tgacaccgat
    50461 cgtttttgtt ctgttcaatg gtgttttttc tttcttatag ctgtcatcta catatgtgcc
    50521 cgaccctttt tctttacgca gatagccctc atttaccaac acagcgattg cttgccgaat
    50581 cgtatgtctg ctgacgtcat atatttcttg cagcttgaat tctgagggaa tggttgtatt
    50641 tacttcgtat tcctcacgaa ggatcttttg ttttatctct tctgcaatat cttgatattt
    50701 tgccatttgc cttgcccctt tttatttgta cgtacatata tcataacgag tctgcaaggt
    50761 ttattcaagc aaaaatcaag gtttcttgtg ctcataaggc atacggaaca ggttcaccaa
    50821 attctggaaa tccttgctca ttccacgtaa agcattgcgc gttcgcgtgc cgattaggat
    50881 tgtctaacgg atcccctttt tggttcttct ctggtcgagc atgatagatc aaaatatcaa
    50941 agcacccgtc ttctgaaacc gtaaacgagt tgtgaccagg accaaattgc ttattttttt
    51001 cagaagaaac gaatactggc tccgataatt tatgccatga ttcttttaat aaaagattct
    51061 tgtgctcatc agcccacagc aggcccatgg catagttttc atctgttgca ctaccagaat
    51121 acgtaatgaa tattttccca ttgcgagtaa ggactgctgg accttcattt actgaaaaac
    51181 cccgtttttc ccattcatac tcaggtatag aaaggaggat ctgcttcccc tttaacgtcc
    51241 aaggattatc catttcggat agataaagat tagaattccc agtaatttca ggatcttgtt
    51301 gggcccacac ataatatagc tttccttgag aagagaaagt tgttccgtct aaactgaaag
    51361 tttccctttt cgtttttatt tgtccctttt ctatccaatc tgtattcatt ggatctatat
    51421 gctgattttc aagtacgaac atgcggtgat gatgagattc atcacgtata gttttatgat
    51481 tgcttgcagc aaagtaaata taccattttc cattgatata atggacttca ggtgcccaga
    51541 tcaattcact catcggtcct tcttcatgag cctgccatac aatcaggctt gcagcatgtt
    51601 ctaattcatt cagtgacttt gcccttctta attcaatcac ttggtagcct ggaaccgatc
    51661 ctgtaaaata ataataacca tctgtatgtt tatataccca aggatctgcc cgttgccgaa
    51721 cgataggatt attataaagt gtcttcatca taacaaattt cctcacattt tctttattct
    51781 aattacattc caagagtatt tttctatccc tacatttaat tcgtcatgtt tttctattga
    51841 aaaatagttt atttcaggtt ttattttttc tggttcttca cgtgtatttt tgtccaacag
    51901 atcgctactg gttaatgtgt agtgctctcc ttgatctagc aaattgaaat catgttcaat
    51961 cgttatcttt tgttgttcat ttgtgcgatt gatcaagaaa atagtcagct gattgccgtt
    52021 atctacgatc acactgtcca cataaggaac attttttatc ttttcacaat cataaacagg
    52081 gcaaatgatt tgttgtttca atacttgacc tttagcatat ttcgataagt gttgcatcac
    52141 ataataagtt ggattcctcc atgcttgtcc tttagcatct gtcagtatta aaggtattgt
    52201 gtttactaac aatgattgac aactaatctt tacgcgatct gcgtgtcgta aaatagccag
    52261 ccctaatgaa ccaaataata atgtatcttc catagtaaag taacaccaat cgatgggact
    52321 accgacttca aaatccctat gtcttttttc cggttgggca tgtacattcc attcatcaaa
    52381 cgctagatac attgttttct cacttctcaa atatcctttt acataatcac aagtagcaac
    52441 gatttcttca atttgacgat cgaatcgata agcttttgca aggtatgtcg ctgtatcatc
    52501 acgctcattt ttaggaggtt tagtcaagtc ttcttcatca tacttatcaa tatagttatg
    52561 caaagctaaa taatctacat tctcataagc ttcttctaat gcgatacgat cccattcagg
    52621 gtacgatggt aaacgtggcg tagagcttcc aactaagatt gtttcaattg aatcatccac
    52681 taacttcatg acttttgagg cttcatttgc taacctacca tattcataag cactcttagc
    52741 ccccacttgc catggaccgt ctagctcatt ccctaaacac cataatttaa cgccaaaagg
    52801 ctttttttgc ccatttttta ttcttaaatc actccagaag gttccttttg gaaaattaca
    52861 atattctact aaattttttg cttcatcaat tccttttgtt cctaaattca ccgtgaatat
    52921 cggtttcgct tgtacatgac tgatccattt aaaaaattcg tggagtccaa aagaatttgg
    52981 ttctatacct cgccatgcta aatcaatttt ttttggtcgt tgttctttcc tacctacagt
    53041 atccacccaa tcataaccag atgtaaagtt tcctccagga tagcgaatga cacctaattc
    53101 taattccttt acataagaga ggacatcttg tctaaacccg tcaccatcag ctgtttcatg
    53161 gcccggttca taaatccctg tatagacgac acttcccata tgctcaacaa atgagccaaa
    53221 cagccgagaa ctgatctctc ctaccgtaaa atcctctctg atttttactt tcacttcttc
    53281 gtccaacagc tttccctacc tttcatttcc tatagtcaag ataatagata caagtttcca
    53341 tacgccacca gcaaagataa aaataaaagg agggaaaaga aatatgagca gtacaaatcc
    53401 taataacaat acgcaacaaa gaatatttct caaaaaatca aaaaatactt tccatgcact
    53461 ttgttcaaaa atttgtaaaa atgtgatatc tttgttttca gaaatcacat atatccagga
    53521 taaaagaaac atggaataaa taagtaaaac tacatatcca ctaattgaaa gtacgattgt
    53581 ctgttcttct aataattgat taacaagaaa ataaataatg atcgttaaca gactaaggaa
    53641 caagttctta ccaaaatgtt gctttattcc ttgaaagaaa aaataaatga tatttcctga
    53701 tccctcacct ttccattgcg ccattacata aaaaacagca catgtagcag gcacgatagt
    53761 aataacaggc aaacacacga ttacccagag aatacctaat aagatgtaat cagaaaccac
    53821 aatcaactgt ccataaagtt tagtctcaac tattttcatc ttcctcactc tttcattcca
    53881 gacatagctg ttccttgaac aaaatatttc tgtgcaaaaa tataaaccac tagtaatggc
    53941 agcattgaaa taaccgttcc agccattaca ggtccataaa aagaaatgtt ctgccctgta
    54001 aataaagcta accctgctgg taaagttctc atttttgaac tacttgtcaa tatcaatgga
    54061 taaagcaagt cattccaact attcatcagc gtgaaaatcc ctaatgataa aagagtaggt
    54121 ttgctcaatg gcaacatgat tttcaaaaaa atattaaact cacttaatcc atcaatgcgt
    54181 gctgcctctt ccaaagaatc tggtaaagta ataaaaaagg aacgcatcat gaagatacca
    54241 aatgcagttg tcatacgagg aatgatcaaa cctgcataag tatcaagcat gccaagcaaa
    54301 ttgacttcaa taaatagagg aatcatgaag acttggaatg ggatcatcat tgtcagcaaa
    54361 acgaaataga acaaaactga ttttcctttg aaacgcatac gagcaaatgc ataccccgca
    54421 agagaatcaa agaatacaga agtgatcact gtccctccag caaaaatcac tgtatttttg
    54481 atgtaatcta ataaagggat cgttgtccaa accttaatat agttatctag ggtatagcta
    54541 tctgcaaata ttctaggcgg aaatgagata atgtcttttt cgtgtttaaa agaagaaata
    54601 atcagccaca aaaaaggaaa cgcaataacc aacaatataa ctagcatact aatcgttcga
    54661 atgatttttt taccactaac tgcttttcga ctattcacag taactgtaat cgagctttct
    54721 ggtgccatac aggtttccct cctatttcat ttgtttttct ttccgtgaca tgtacacatt
    54781 agtggagata gctatagttg caacaatgat aaacaaaacc gaagacagtg ctgatgcata
    54841 gcccaattca taaggtgctg taaatcctcg actgtaaata tattgaacag ctgtttctgt
    54901 cttaaattgt gggccaccag cggttgcaac atatacttgg tcaaacactt gcatcgaacc
    54961 aatcagattc gttaaaatac aaaaacctaa ggagggaacg atctgaggta gcgtaatgtt
    55021 aaaaaactgc tgtcgatttg ttgcaccatc catttgagca gattcataca aagagggaga
    55081 gattccctga atagcagtca agagaatcgt catagtaaca ccaaaattct tccaaacagt
    55141 catgatggct accgtcggca tagcaagggt tggatcgcga aaaaattgag ggatcggcaa
    55201 gccgacttgt gaaaacaagt aaggaatcgc ccctacattc ggatcaagca acatagaaaa
    55261 aataatcgca attgacgtca atgaacaaat aactggaatg taaaatgtcg aacgacaaaa
    55321 acgattgaat ctggtatttt tagataatgc tactgctgct aataaaccaa cgataatttg
    55381 tgttggcgtt tcgagtacgg tgaaatataa agaatgaatc aacgaattaa ctgctcgttc
    55441 atcactaaat atacgtgtga aattttcaaa acttacaaaa tttcttgatg taaagaaaat
    55501 attcaaatct gtaaaactaa tagcaaatgt tcctaaaagc ggtatgacag taaataataa
    55561 taaaactaaa aaagatggag ttaaaaacaa atacgcagca cgatcaggac gattccaata
    55621 tttggtgaat ccgttcatat agtccacctc atctttcatt ttgataaaat atacaatctt
    55681 ggtttaaacg aatctgaata agatatccga acaatcgctg tgatttttgc aataacacct
    55741 atctgcaaat actaggtcga tcattcagat atctgtatct tgccaacagt ctatcctgta
    55801 taaagttctg tactattttt gcagtaattg attgatggca tcatctgcat cgttcatgag
    55861 tgtctcagga tcttctcctg ctaataattt ttcaatcagt ggattgatga tatcgttatt
    55921 gatcgcagag atttgggtaa aacctggtaa atatggctct gcatagtcca tttgtttcga
    55981 taattcagat acaataggat cattttttac ttcttcatct tttgctactg aatccaagta
    56041 ggcaggaaaa ccattttcga tactccattt tttaccaatt tcagttgtat tccaatattt
    56101 caaaaactca tagacagctt cttttttatt attatctgta cttgccggaa tggcatatcc
    56161 tacgccatct aaaatagctg tttttttgtc tgaatcactc ccttgtggta aggtagtcac
    56221 attataatca atctcgtttg ctttcaatcc gttatttaac catggcccat tgatttcaat
    56281 ggctagttga ccagcattca ttaaattgtc catttcggcg ccagaaatcg attctggacc
    56341 agtcttgtct acatggatca ttttttggat cgcttttaac gttttaaggt tctcaggaga
    56401 cgcaaattca gacttagtat aatcatcgtt cactaattta ccgccatttg ctaacatcca
    56461 gttatataac ggtgcagttc catccttatt gaagacaaaa cctgatacat ttttactact
    56521 atcagttaat tttggtgcca tttctgtcaa ttcttcccaa gtagtaggtg gttgttcagt
    56581 atctaatcca gcttgtttaa acaaatcatt attccaatac atatacatgc cttgtacctg
    56641 catggggaca aaatactgtt taccttctac ttcacctaat tcaatagccg tttctttaaa
    56701 atcagattta tctactccat catattccca gaaatcgtct aatggttgta ccgctccgtt
    56761 ttctacatac tgtgcaaaat ctggataatt taatgcaata aagtcaggtg cttttttaga
    56821 agaaatcgct gtaggaagtt tttcattcaa attggcccaa gtcatgatat ccatttcaat
    56881 tttgatgttt ttatcatttg ttttattaaa ttcattaacg atttcttgca atatttcacc
    56941 atcagatgca gtaaacccat tccaaaaagt caaagtcact tctttatcag aatcagattc
    57001 tgctgaactt ccgccaccgc aagctcctaa aatacctgtt gcagctacca acaaagagcc
    57061 aattaaccat tttttacctt tcattttttg ttcctccttc atctgataaa cgacatctga
    57121 ccacattcca agatagcggt ttcaataaac aattgatttt cccatcttca gcttttacat
    57181 ttgttgaatg acttggtttt acttgttcac ttttaggaag attagttgct tttgtatcaa
    57241 atccactcat ttcagaaaaa tcaatcactc cctctaatga taattcagac aagtgaacag
    57301 ttagattcat ttcttcttct ccacggttaa ctgcaaatat gacaagttct ttgtcattta
    57361 ttacggctat actttctaaa tacggcactt cttcaaaatc ttccgtcgca tagtttgctg
    57421 aacgaatttg tgggcttaac acctctcctt gtccatacac agcagcttgc ataaatggat
    57481 aatagatcgt ctgtctccaa gcttctccgt ttgtttctgt cctgattggt gcaataacat
    57541 ttaccaattg agcaagacaa gcaatcttta ctctatctgc atgtttcaat aaagtgatta
    57601 atagacagcc taatagtaat gcatcttcaa aattatagtt atcttctagt aaaggaggag
    57661 caatctgcca aggcgctgct tgctcatctt gttccgctga atgataccaa atattccatt
    57721 catcaaagga caggttgatc tgcttcttac tatgtttctt cgccttgact gtatcgcaca
    57781 tcgctgtcac ttcagaaatg aaccgatcca tgtctaagga acgtgcaaga taatgagaaa
    57841 gatcattttt ttggttgcca taatattgat gcagagacaa ataatctatt tgatcgtacg
    57901 cttgatcaag aactgttaac tcccaatcac caaatgtagg catttgccga ttcgaacttc
    57961 cgcataaaac cacctcaatt gaatcatcta cgagtttcat cgccttagcc gtttcctctg
    58021 ctaaccgtcc atattcttcc gctgttttat gtccaatctg ccaaggaccg tccatttcat
    58081 tccctaaaca ccatgtctta attccaaaag ggctctcttg accattcgaa cgccttaaat
    58141 cactccaata agttccttta ggaaaattac aatattcgac taaattacga gctgcatcta
    58201 ctccacgagt acccagattg acagccatat tgatttcggc ccccacatgc tgacaccagt
    58261 tataaaattc atgaatccca acttcattcg tttcaattgt ccgccaagct aaatccaacc
    58321 ttctaggtct atttgctatg ggcccaatac tgtcttccca acgaaatcct gacacaaaat
    58381 ttccaccagg atatctaatc atgggaatgt tcaattcttt taccaagtga atgacgtctt
    58441 ggcgaaatcc acttttatta gctgatggat gatctggctg ataaatccct tcatatactg
    58501 ctcgtcccat atgttcaata aatgaactgt acaaccgcgg atcaactttg cctacacgct
    58561 tgacactact aatcaacatt gtggcttcca taactgacct cctttaattt atacatgaat
    58621 aatacacctc gttgaaagcg ttgtcaataa tataaatgtt aaacttactt attttttatc
    58681 gattttaatt aaccaaaata ttaataatga ttaaaacaaa taatttaata taacttttat
    58741 taacaaatat gttaatatat aagaaaaacc ttttaagagg tgatatatat gcaactacaa
    58801 ataaacgaag aatccttacc ggtatatgaa gcattagcta gtaaaacaag gatcaagatc
    58861 atccagctac tttccaaaaa aaagatgaat gtcaaagatt tggcaaaaga acttggagtg
    58921 agcagtgcta tcacgacgat gcacgtgaaa aagctagagg aagcaaacat catcaaaaca
    58981 gaaaaagtag gacaacaaaa aatttcgagc cttcgtgtcg ataaaatcga tatcagtttt
    59041 ccagaaaaaa tattcaatgc ttttgatacg aaagaaacat cgatacctat tggtcactat
    59101 acaaattatg caattgaacc aacatgcggt ttagctacga tccatgattt tatcgggaaa
    59161 gtggatgaac cacgctattt tatggatcct cgaaggatgg atgcccgcat tctctggttt
    59221 accagcggat ttgtcgagta tcaggcacct aattttttaa atacagagga tacattggag
    59281 atgttggaag tttctgtgga aatttcttct gaatttcctt tttcaaatga caattggcct
    59341 tcagatatca ctttcagcct aaatggtgtg gagctaggaa catggacgag ccctggagat
    59401 tttgctgata tccgcggtaa atatacgcct gattggtatc cagataatct taatcaatac
    59461 ggcttgctga agacgatccg aattaccaaa cacttgacga atatgaatgg agaacccttg
    59521 tcaaacatca cgatcaacga catccctaaa gagcaagaca cttggcattt gcgaatcgaa
    59581 gtgaaagacg atgccaaaca tgtggggggc tgtactttat ttggtaaagg attcggtaat
    59641 tacgatcagg atatcaaatt aaaagtttac tacagctgat atttttcttt tctattttat
    59701 aaaacaaaaa cagccaatcc ctatgaaggg acggctgttt ttgctttatt gttatttcca
    59761 gaaatcatcc aaaacggaaa ttggcaaatg tcgtttatgt tgtgtcttgt tccaccatgc
    59821 ttcgattttt tcttgtgctt gagcgggaac ttcttttcct tctagataat catcaatttc
    59881 attatatgtg acacctagtg cgacttcatc tgcaatcatt ggtttcccat cttcaagatc
    59941 tgcagtcgga atttttgtgt ataatttttc cggtgcattc aatgcttgaa gcaattgttt
    60001 cccttgtcgt ttgtccaaac ggaataatgg caaaatgtcg gctccaccat caccaaattt
    60061 cgtgaagaat ccagtaatgt tttctgcagc atgatctgtc cctaatacgg ctcctgctct
    60121 ttctccagct acggcatatt gcgtaatcat acgttgacgt gctttgatat ttcctttgtt
    60181 aaaatcgctg attcccacac ctgcttcgct taatacttgt acttgtgcat caacagctgg
    60241 tttgatattt actcgcagac taacatcggg ctggataaag tcaagagcag cttttgcatc
    60301 ttcttcatct gcttgttcac cataaggaag acgaacagcg ataaattgat agttttcatc
    60361 ttttgtttct tcacgcattt cttccatcgt tagttgcgcc aaacggcctg ctaaagtaga
    60421 atcttggccg ccgctgatac ctagtacaaa agttttaaga aacggatatt ttttcaaata
    60481 tgctttcata aagtcaatac ttttacgtat ttcttctttt ggatcaattg ttggtttgac
    60541 acctaattca ttgataattt ctgtctgcag gctcatttat tctcctccta tgcttctgta
    60601 tgaagttctg ctacgctttt tttcattcgt tccatgatag ccattttatg gttccagcat
    60661 tcagtagaca gatcgactgg gtatttttgc ggattcaagt ctcgtttata ctcttcccat
    60721 agtgaatcta gattttcttt tgtatattct ttgatttcgt ttagtgcagg taattcgtaa
    60781 actctttttc cttcgataaa gatatcttgt aaaactgggc gagctgtaaa gtctcgaaca
    60841 gttttattga taaacgtatg cactggatgg aacatgaaga tagcttcttc ttctcgaggg
    60901 tcttcatccc ataatgtcac gtagtctcct tcagatttcc catcaaaatt tcgagtgatg
    60961 cgccacactt gttttttccc tggagtagtt actttttctg cattgcttga taacttgatc
    61021 gtatccatca tttttccttc atcatcttcg atcgatacta gcttgaatac tgcaccaagg
    61081 gctggctgat cataggctgt gatcaatttt gtcccaacac cccatacatc aattttagct
    61141 ttttgcatct ttaaattcaa aatcgttgct tcatccaagt cattcgaagc atagattttt
    61201 gcttccgtaa atccggctgc atctaattgt tcccgaacac gcttagatat ataggccata
    61261 tctccactgt cgatgcgtac gccttggaag ttgattttgt cacctaattc tttcgccacg
    61321 cgaatcgcac ttggcacacc tgatttcaac gtatcataag tgtcgactaa gaacacgcag
    61381 tctttatggg ttttagcgta ggccataaat gcttcataat catttccata agattggact
    61441 aacgaatgag catgggtccc gctagctggt atcccaaata tcttcccagc acgtacattg
    61501 cttgttgcat ctgctccgcc gatatatgcc gctctagttc cccagactgc tgcatccagt
    61561 tcttgtgccc gtctagttcc aaattccaat agtggatctt ctccgatgac agatttgata
    61621 cgagctgctt ttgtcgcgat caacgtttgg aaattgacca tattcaacag tgctgtttct
    61681 actaattggc agtgcgctaa aggcccttcc acttggatta atggttcatt tgcaaaaact
    61741 agttctcctt cacgagcaga acgaactgtt gctttaaatt caaaattttg taaatactcc
    61801 aaaaaacctt caggatagac ttctaatcca cgcaagtaag caatatcgga atcattgaac
    61861 gtcagattct ctaaataatt gactaaccgt tccaatcctg caaaaaccgc gtatccgtgg
    61921 ttaaaaggca tttctcgaaa gtaacattca aaaacagcat gtaaatcagc tcgacctaat
    61981 tcccagtatg tctgcatcat attgatttga tatagatccg tatgcagcgt caaactgtcg
    62041 tctgggtaag tattattcat cttgtattac tcctcttggc aagttttcat ttcctaccca
    62101 ttataacaaa aaaaattaat aggaaaagaa atcttccatt ttttctgaaa aagcacatgt
    62161 tttacggata tttcttgcta aaaatgaact ccatctgcta aaactatcct ttcttattgg
    62221 tatttaacct aaacgagaaa gaatacttat tttattcaat gtatccgaga cacgaagaaa
    62281 ttagcgtttt ctatttttct tacttgataa aataagtaga ttttcctact acaatcgtag
    62341 ccgttaagat accgatgatt gaacaaatat aaccaaatgt ctctacaaca taatcaatgg
    62401 caaaagtttt tgaagcaata atacgtcttt ccttttttgc ttcttatccc caaagaaacg
    62461 gatataactt attatgtatt gaattttttt ggaaaggcga attatgacaa gatatacctt
    62521 gcgttgaaaa aactcgcgga aaaaatatca cacgttttta aaaagataca aagtaagaaa
    62581 gcagctgtca atagaatgga aacaaaaatg gcgagacatc aatcagtctc gccttgtttt
    62641 ttattgtata cattagatat ggagcaggtt accataaaga tgattattag tcgttattaa
    62701 atagaatcct tcgtattttt ttgttctatc actacaatat ctttatttgg acgtttgaat
    62761 tgtattccgg cacgttctag ctcctgtttc actcctacat ggactttgac atcttctcgt
    62821 gtcattttta aaacgtgatg aatcgcatag tcgatttctt gccttgtgat ttttttcttc
    62881 gtattgatca tcacgacttg ctttttatca tatgtgtcta ccaatagcag ttgtgttcga
    62941 tctaacgaaa ataatttgta ataattaata gccgtattcc caaaaaagaa ttccactact
    63001 ttaggataaa atgtcttaat atttaaatta ctattcacag gtgtttcgat tagcttcatc
    63061 tcattcctct tttctatata gtcgatttag atccaatctt aagttatgtt accatctttc
    63121 ctagcattaa ggaatcggtt acaattgaac ctttaaagac aaaaggattt accttttatc
    63181 acttaatagt ataacataat ttcgtgagaa aaaaatacaa ttttttcttg accatctcgt
    63241 tacgtaaagg attatgctta catttgtcag gaggaaaaat aatggagtac acaataaaga
    63301 aaatgtcaga gatatcagga gtcagtccga gaaccttgcg cttttatgat gagattggac
    63361 tgctaaaacc agctaggatc aattcttcag gttatcggat ttatggcaaa aaagaagtcg
    63421 atcgtctgca acacatctta ttttatcgaa caatggcatt caagttggat gatatccaag
    63481 aggtcttaga caatccatct tttgaccacc agaaagcttt aatcaagcat cagcaaatgt
    63541 tgctggaaaa acgggcacaa atcgataccc tcttgacaac tgtccaacag acacttgata
    63601 tgtacgaagg aggaagaaaa atgagtgaca aagaaaaatt tgaagggttt aaacaacaaa
    63661 agttgaaaga aaatgaagaa tcctatggac aagagattcg acaaaaatac ggaaaaaaaa
    63721 aggttacagc atccaatgaa aaatggatga atatggataa agaaacgtat gaagaaatgc
    63781 aaactgttga aaagcagatg ttatttgatc tcactcttta cttaaaagaa ccagaagatg
    63841 aaagcttagc agaccgtatc ttccaagctc ataagaagtg gttctcttac agctggcctg
    63901 aatatcagcc aaaagcgcat aaaggactag gaatgatgta cgaagccgat gaacggttca
    63961 ctgcatatta tgacgagcga agtggtcatg gtgcagcaaa agctttaaat gaaattatcc
    64021 aaaaaaaggc gtaatctttt acatgatcca acaaaaaagt acacgtaaaa tgacctaata
    64081 acaaaacagc agacacccaa gttagatttt tatgctaact tttggtgtct gctccattct
    64141 ttcacaagtt ttttcatcct actttaaata tattcatcgt tgaataaacg tataaggtat
    64201 tttcacttta tccttttttt ctgtaagaaa caattgaaat gccatttctc cgcaagcttc
    64261 cagatggtgg tcgatggttg ggaaaccaag aagctcgctg atcagcaaat tatcgcgtcc
    64321 aatcaccact ggtgtcttat cttgcagata gctttgcaag atgcctgctg caaccatatc
    64381 cgaatttgta aagacaccat caactccaag ctctttaaaa aagaagcctg cttgttttcc
    64441 tttctctgca tctatacaat cccaaaaaac aagtgattca tcaaaagatg gaaaaagttg
    64501 atgacatagc tgcaaagtga tttttgagtt ataacttagc ctgccacttc gccccaacgt
    64561 gatgcccaat tgctttatac cttgctgctt caaataagta agaccttctg taattgattg
    64621 ttctcgatca ataaagacac agcttgcctg ttttcccttg atttcttcac aaaaaactag
    64681 aggtccatat ttttgatatg ccaataaatc atctagtgta ttggcacgag acgtaatgat
    64741 caatccatcg aaagctttag ctgcaaattc ttcaagatac cttcgctctt tttctttatg
    64801 atagcccgtc ggtaaaagtg tcactttgta atcataatca aacgctgctt caatgatccc
    64861 actgatcaac tgatcaaaat acggatgatt ggtataaggc agaatcactc caatgctttt
    64921 cgtttttcca taacttaact gtctggcatt tgcattaggg atataatcga cttctttcat
    64981 gaccgcaagt atttctttcc ttttttcctc atctacatat ggatgtccat tgattacacg
    65041 tgaaatagta gaaactgaat aacctgtgag ctttgcgata tctcgaatgt ttgccactgc
    65101 tgcttcgcca cctatacaaa aagattttct tgtaaatagt ctaccacagc tggaccgcat
    65161 tcaaaaaact gatacatttt tccttcatgc gaaaaactga tccgctgccc gccccaatat
    65221 atatcttttg tcaaattttc gatttcattc attggtattt cttttactat attcctattc
    65281 aacccttgaa aactgtacaa cgttaatacg gatcccttga actcgacaaa cgcataaaaa
    65341 taattgttcg ttgtgccatc tgttatctct acataagtat tcattgcaag ccctcctttt
    65401 gatactttga gtatagtgta tgaaacgcgt ctcatagcaa ggagtttgtt ttgccaaaga
    65461 aaagttttat tcctctttca ttattaccaa agtctcttat tttaaattga atacattata
    65521 atgatactgt aaatatatct ttattattag tgaatggagt caagtccaca atcttgtgta
    65581 aaagttcaac ctcatcaaaa gcacaatgtt ttgcgttgtg aacctaactg ccatgccttt
    65641 ctttctgagg agagaggtca ggagcagtag cgcagcgact cttgatgtct ctcctcagaa
    65701 agaagagact cgcacaggtt catggtgcta aatgttattc gaattaaatt ctaaatattt
    65761 tcgtggagaa gccagccact cagtgtcttt ttccatcaaa agtgttccaa ttatccgagt
    65821 gatggaattg atatttggaa aaatccgaat cactcgttct ctccggcgaa tctcttcgtt
    65881 caaccgttcc aacacattgg ttgtacgaat ccttcggcgg atattttcag gaaaagacaa
    65941 aatttgaata gcatcttcaa agccgttttc taaaatgtcg caagcagcac ttaattttga
    66001 atcacaacca tatttttcca agaagtgctc ttttctttca cgtgtcaatt caagttcaga
    66061 ggctttaaag atactcttca actcgtcttt tacatcagaa gaaactttct ttggcagctt
    66121 atcaaaaata tttctcaaaa agtgggcttg gcatctttgc cagctcgcac ctaaaaagtt
    66181 cttgcgaaca gccttaacta accctttgtg tgcatcagaa atgaccagtt ttacaccgaa
    66241 caaacctcgt tgaacgaacg attgaaaaac ggtatcccaa ttatcttccg attcgccgtc
    66301 ttgaacatca aaaccaagaa tcgtacgtcg tccatcagaa tcaatgccga taccaatata
    66361 tacacctttt gagacaatac gatgattttc ccttactttc atatacaggg catcacacat
    66421 gaaaaatgga tattccgagc cttctaggct ccggtttctc cactcaaaaa caaatggatc
    66481 caactgtttc atcagatttg aaacgaatga tttagaaaca gtcgcaccat ccgttagcag
    66541 ttcaactgtt ttagttactt tgcgagtgga gacaccagaa atgaccattt caatcatggc
    66601 agcgatcaaa gctttttcgc ttcgctgata tctttcaaag atagtaggac taaacttacc
    66661 gtctctggtt cgtggaacat gaagttctaa cgtaccaatt cgagttgtaa acgaacgatc
    66721 gtagtaacca tttctttgtg attgccgttc ttctgaacgt tcataggcat ttgcctgaat
    66781 atattgattt cgttgttctt ccatcagctg attaaacatg atagtcataa gtgtttttgc
    66841 ggtatcattc gcaccgcttt cgtttataat tgcttgaatt tcttcgcttt caaaagtaaa
    66901 atgtacttga gtcattgata tcagtcctat tcgagtatgt tttctgcaaa aaacattgta
    66961 ctcctaactg agatcaatgg cttttttgat gcttcacttt tacacaatta tatggacgta
    67021 atcataagtg aaagcgtttt atataagaac ttgaaaagaa agaagggata gacatgaaaa
    67081 aaagatacaa attggtgata gctttgctaa acattaccct attattcagc ttaagtgcat
    67141 gtggaaaaac taacgaagaa gtaacagcaa acatcgcaga tccaatgata aaaaaagcca
    67201 ctacattaga agtatatgtc gcaaacggca aactgacaaa ctatgttgaa aatgctgcaa
    67261 aaatctataa ccgcaaaact ggagcaaata ttgacttaaa aattgtgaat gtagcttcgg
    67321 gaacagcaac agtacaaatg atcaccccaa aattggtaac acacgaagat atgccggata
    67381 ttatctccat ggcagacact tcagcagcag gtgtgctgga aaaatttcaa gatgcttttt
    67441 atagtgctga tgaatatgga ttttatgaca aatatggcga tcagttttat gatcaaaaat
    67501 tgaatatttt gcgtcaacaa acgagtaatg aaacgattat tccttgggct agtgatttta
    67561 ccccagcact ttgttattat caaccaaaaa aatttgctga aataggtgta gattttgaag
    67621 aaatcagttc ttgggaagaa tatatccagg tggcaaaagc attaaaagaa aaaactggta
    67681 gttttggaat tgccttgcca gaatctgggg accaagaatt gtttatcaac atgctagccc
    67741 agcagaatca atctttgctt gatgaagaag gaaatatccg tctgaatact aaagaagcga
    67801 aacatgctgc aaagttaatc aaagaaatga tcgactcaga tattgtacat ttctacggtg
    67861 cacaagatgc tgagaaagca tttcaagaat ctgcaatgtt tgttgctggc ggctggtatg
    67921 ctacgaatat gtctttgaat tttcctgatg catctgacaa atggcgcatg gctccactga
    67981 ttcctttttc aaaagaaaat ccaggaaaag gtgcagtatc aggaggatca agtttttatg
    68041 tccctaaaga tgccaaaaat ccacaagtgg cacaacaatt tcttacattt atgctgacag
    68101 atgaagaatg cttagccaac gcactggagc tgggcgtggc tacatctaac tcattggcct
    68161 atcaaacaga agcagctgag aaaaagtttg actactaagg aggacaaaaa tactatgaac
    68221 tattgaataa aatcaatgaa aatacatcaa atactgtttt accagccact tattcagatg
    68281 ctacgtctta tttgactacc gctgcatata actattggca aacaggtgat tttgaaaatt
    68341 catatctaaa agaagcagaa aattttgctc ataagtacgg cgtaaaatta actggtgatt
    68401 aaaaaatatt aggaggactt aacgatgatg caatcttcgt taccaagcaa gacaataaag
    68461 aacagcaaga aaaaagcaac agatacgtct cataaaaaaa gtgcttatgg attggtcaca
    68521 ccttttgtta ttttctttat tgtgacgatt ttgattccca tcattatctc gtttgctttt
    68581 agttttatga aaattggcta ctcatttgaa tttgttggat ttgacaatta tctagctatc
    68641 ttccgagatc cattgtttat taaatcttac acgaatgttt tgattatcat ggcaggatca
    68701 attccaataa cgatgctatt agctatttac tttgctgttt tattgaattc agcacagtta
    68761 aaaggaaaag gattttttag aactgtctat tatattccaa ccattacatc aacggtggct
    68821 gtagcttccg tattcatgac ttttttcaat ccgactgggt tattcaatgg atttttgaat
    68881 gcgctaggaa ttaacggtgt acagtggtta acaaatcctt tttggatacg aatatcaatg
    68941 atcatcacga tgatttggct tagtgtagga tataacacta ttttattttt agctggtctt
    69001 caagggattt ctaaagagat atacgaaagt gctgaaatag atggagcatc gaaatttcgg
    69061 caattctttt tattaacgat ccccatgcta aagccaatca ttttaatggc cacagtttta
    69121 gccactatca atggcttggg taatttcaat atcccaaata tattctttgg gacttccaat
    69181 ggaccagaaa attcagcttt ggtcgtagga gtcaatttat ataaaacaag ttttgaaatg
    69241 gttgattttg gaaaagcctc tgctattgcc tggacgatgg tgtttgtatc tgttatatta
    69301 tcggctattc aatttaaatt cggaggtgat aaaaatgact aaacaaaaga tacaaagcag
    69361 aatttttacg tacgtcatta ttgtgtttgg tgccttaata tttttgatgc catatttata
    69421 tatgattgta gcttcaacgc aaagtaatca agaaatttta agtggcggaa tcaatttcaa
    69481 gtttggggat atgctattaa aaaactggca agccatcatg gcgagatata attatccctt
    69541 gattatttgg aattcgattg ttattgctgt aatcagtaca gttttagcta cagccagttc
    69601 tacaatggcg ggttatgcat tagcaaagta tcgttttatg gggaataaac tgttgttcaa
    69661 tacagttatg ctatctagga tggtgccttt ttttaccaca ttgattcctt tattttacat
    69721 tatgagtcgt ttaggattat cgaatactta tgcaggtgtg atcatccctt cgattgccag
    69781 tacaacttca gtgtttatga tgcgacaata tagcagtcaa tttcctacag cactgatgga
    69841 ggctgccaga atcgatggtg ccaatgagtg ggtgattttt acgaaaatcg ctgtaccgat
    69901 tttatcacct acgattatta ctagtggttt acttatcttt atgggaagtt ggaatcaata
    69961 tctatttcca ttagtcatgc tttcagataa agataaatat acggttccct taattatcaa
    70021 aaatatgacg gtttctagta caggtgagcc aattaattat ggcgcaatta tgctaatttt
    70081 agcaacttca gtgatcccaa tggtcttgat ttatgtgtgg gcgcagtcta aatttaagga
    70141 gaatgacatt ggagctgcaa ataagtaaaa aaaataacga atgttaacca tctagttcgt
    70201 tctaggagca actccacatt cataaaaatt aaaagaatgg cgggatatcc atgaatgaac
    70261 agacgaagga atggtcaaga aaaattttga atgtagcttc tgaaaagata gatgcggaat
    70321 tagacagaat ggggaataaa attccatata tttcttacaa cggaaaatat acagacgtaa
    70381 taaaggacag aggaatcgac tggtggacca atggtttttg gagtggtatt ttatggcagc
    70441 tctttcacaa gacaaaacga gaaaagttca aagaggcagc aatacaacaa gaacaattat
    70501 tagaatctgc cctgtaccat ttttcagacg ttcatcatga tgtaggtttt ttattcttac
    70561 catcggctat tgcacattat caacagtctc aaaacaaaca tagtcgacaa ctagggttac
    70621 atgcagctac ggtacttgct ggacgctata atatttcagg gaactattta gtagcatgga
    70681 atgaccataa acctggttgg atgattattg atagtatgat gaatttacca ttactgtatt
    70741 gggcttcgag ggaaactggt gatccgaggt tctctaaaat tgctcggcag catgcagata
    70801 caacagctaa gtatctagtg aggaaagatg gatcaaccgg gcatatcgca gaatttgatg
    70861 cagaatcagg gaaatatatt cggttacttg gcgggcaagg atacaatgag aattcttgtt
    70921 ggagcagagg aaatgcatgg gctttatatg gattcgcgtt aagttatcgt tacacaaaaa
    70981 atccgtacta tctagctata gcagaaaaga tagcagtaaa ttttattcag aaaatcgaaa
    71041 aaactggtta tattcctctc attgattttg ctgtaaaaga ttcgaatatt gataccagtg
    71101 ctggagtatg cgctgcatgc ggtcttctgg aattagcagg atatgtttcc tctgtacgat
    71161 caaaaatgta tcatgaagct gctatcaata tactccaaaa gacagaaaag cactatgcaa
    71221 attggaatat ggatgaagat gggatcattg gaggcggagc agaagcatac catagagaac
    71281 tgacttataa tgtgccactg atctattctg attatttttt tattgaaggc ttacttcgat
    71341 tagaacagtc ggaattagct atttggtagt aaaaagagga gaaaatacaa tgaaacatcc
    71401 agtaccacta ttaaggacca gacaggatta ctttgactta tttaaaaaga taaatcaacc
    71461 actccagccg ttttatcgag caagtggcgc acaaattgat tatatacatc aaggggtagg
    71521 atatggaaat aaaattgcag gaatggaagg attctctcgg gttttatggg gagcggggcc
    71581 cgcaattgac caattagatg aacgatggtt gaaattaatt ctatcaggaa taaaagctgg
    71641 aacagatcct tcccatcccg attattgggg agaaatacat gaaagagacc agcgtatggt
    71701 agaaatgccc gcaatgttat tagcgttata tcatcatgaa caattattat ggaaaaaatt
    71761 agatacagga gaaaaaagcc agattattca ttggttctcg caaatttttg aatatgaatg
    71821 tgcagatggt aattggcaat tttttaagat aattgtcgga acaattatcc aaaaatttgg
    71881 ttatccagtt aaagaatcag cactgaaaga agcatttgat aatatagaaa attgttatct
    71941 tcaaaatggc tggtatcgag acagttcaag aggtcgccaa gattattata atccttttgc
    72001 atttcattat tatggtttga tttatagcgt actagaacca gaaaagccca ttagccagat
    72061 atataagaac cgagctattg atttttctag agactatatt catttttttg cggaagatgg
    72121 tgcaaatgtg ccttttggtc gaagcatgat ttatcgtttt gcagtttcaa gcttttggtc
    72181 agctatgttg tgggccgatc ttttgccttt taaattaggt gaaatcaagg ggttagtcaa
    72241 tcggaatata agatggtgga tgcaaaaaga aatatttgat gaaaaaggta tcctgaatat
    72301 tggttatacc taccctcaat tgacattgac tgaaccctat aatagtacgt tatctccatt
    72361 atggctgaat aagatttttt tattgttggc gctacctgaa gaccatgagt attggacggc
    72421 taaagaaatg cctatgccta ttcgtgaagg aactaaactt ctttcagaag ccaatttgct
    72481 catacaacat gataatggac atacattttt tttgaatgcg ggacaattag ggccaaattt
    72541 ccatacattg accaatgaaa aatatttaaa atttgcttat tctagtcaat ttggtttttc
    72601 gataccgcga gcgaatcaat tgaaagcaga aatggcaatg gattcaatgc tagggattca
    72661 gcgagcggac acatatatca cgaccagttt taagaatgag agcatcaaaa cctatggaca
    72721 gttcatcgtt cgaaataatg tacgagacat tgtatgtaag aaagaggtac ttgcctctac
    72781 atgggaacct actttacagg caaaaatcag aacttggctt attccttttg aaggctggca
    72841 gattcgcgta cacaaagtag aattagatac agaatatgtt ctatatgaga cagggtttgc
    72901 tattgaatgt tctcctgaga aagaaggaat aatcatagaa gaagacaagg aaaactatag
    72961 ggtgagtgaa gctggctttt caggtattat ctgtttaagt gacgatacta ttaaacgaaa
    73021 atccacagct attatgggtt tgcccaatac aaacttgatg acatgggaaa acacttttat
    73081 tcctggatta gagggtacat ttggaaaagg aacccattgg ctcggaacag gagtggttgc
    73141 tcatcaagat cgggattata gtttagaagt atggaacaat cgtcccaaaa ttgattttga
    73201 tgggacaacc gggctaacta tccaaaataa aaacaataaa aaaagactaa aattatgcta
    73261 aaggctggtg gggaaatgct tgataaagtg atgtttgaag agataataca tccagaaaaa
    73321 tggataaatg aatgttataa agaattaatg gaaaaagaat ggaataatga gatcgagcgt
    73381 tgtcgcagac acacggagag gttaatcaaa cgtatcagta acagtcagta tcaattccca
    73441 agcgagtgtc ctataaaagg acgttatcca ttaagagaca atattggttg gacagaagga
    73501 ttttggacag gaattaattg gttgttattt gagaagacgg gtgatccttt atttcaacaa
    73561 acagctgaaa aagcagatat agattttatc cacaggattg actgtcaaat tatcactaat
    73621 catcacgatc ttggtttttt gtattcttta tcaaccgtag cagattataa aataacggga
    73681 aatcgaaaaa tgaaggaagc ttcaattgat gctgcagaaa cattacttca gcgtttttgg
    73741 gagaaggcag gagttataca agcttgggga gatcctagtg atccttcaca acaaggtaga
    73801 atgatcatgg attgttgcat gaatcttcca ttattgtatt gggcgagtga agaaactgga
    73861 aatcaaaaat ataaaaaagc tgctcaggct catattcaaa atgcagcaga ttatttagta
    73921 agagaagatg gttcaactta tcatacattt tatatggatg tcaaagatgg aaaaccaaga
    73981 tatggaagta cgcatcaagg ttatagtgat aattcctgtt gggctagagg ccaagcatgg
    74041 gggatttacg gttttgcatt aagttatcgt tatttgaaag aacaaaaata ttttgattta
    74101 agcaaaatat tagccaatta ttttatgaat cggttgccag aagataccat ttgttattgg
    74161 gatttgatct ttgatgattc tagcagagaa gaaaaagata catctagtat ggtgatcatg
    74221 gtttgtggat tattagaatt aatagaatca ggtctattag acaaaacaga aaagaactta
    74281 taccagtctg ctgtttggaa aattatgcga caattgaaag aaagatatgc tacaacggaa
    74341 gaagaagatg gctttttaac acagtctgtt tataataagc ccaaaaataa tggggtaaat
    74401 acaccttgta tttggggtga ttactattat ttagaagctt tagtgagaat gactagtaat
    74461 tggtcttctt attggtgaaa aaggtaaata tggaattttt gtttgataaa ataaaaaaag
    74521 aaaaaccaaa acagcatgaa tggtttctgt tttggttttt tcgttaattt tcatattctt
    74581 ctaattccaa gcttagttct tcccattccg tcaattttgt ttcttgatct tgtctagcag
    74641 cttctaaatc ttggttcagc tcattgagtt ttacgtgatc ttccaggttt tctggcgcga
    74701 ccatttcttg ttccaactga tcgatccatt catcgatccg cgccatttct tcttcaacct
    74761 gagtaatttt tcgttgaagg ctacggataa gcttttgctg ttctttgctt tggtaataat
    74821 cagatttgtt cgtgggagca ggagcagatt ctggctcatc ttgtgccaac aattcggcga
    74881 tttcttcttc ttcctgcttt ttctctaaat aataatcata gtctcctaga tagactttgc
    74941 tgccattttc agagagttcg acgacttttg tcgcgatccg attgatgaaa tatcggtcat
    75001 gggaaacaaa aagcagtgtg ccgtcataat cgatcaatgc attttctaat acttctttgt
    75061 tgtcaatatc taaatggttt gttggttcat ccaaaatcaa aaaattttct ttgttcatcg
    75121 ctaacttggc taaagcaacc cgtgcttttt caccgccact tagcagaggg attggttttt
    75181 ctacatcttc gccagaaaat aagaaagcac ctaaagcact tcggatctct ttttctggag
    75241 tggtaggatg ctcatcccac aattctgcta ggatcgtctt gtttgtatgc agatcagctt
    75301 gaccttgatc gtaatagccg atggtgacat ttgtccctag cgtttctttt ccacggataa
    75361 aagggatctg gtgaatgatt gatttcagca acgtcgattt cccgatacca ttcggaccta
    75421 ctagagcaat cgcttcttga cgacggatat ctagattgat tggttcagat aaggtgacat
    75481 tatcatatcc gatagcggca tcttcgattt gcaagactac atttccagaa ggcttttcta
    75541 tactgaatag aaagtttgct tgtttttcat ccccttgagg acgatccagc cggtccattt
    75601 tttctaatgt ctttctgcgg ctttgcgctc gttttgtggt agaggcgcga acaaggttcc
    75661 gagcaacgaa gtcttctaat ttatggattt ctgtttgctg cttttcataa gccttccatt
    75721 cacttgctaa ctgttctgct ttcaaatcta gatatttcga atagttacct ttgtaatggc
    75781 gcatttttct tctgcttaat tcatagactt cattgactac tttatctaaa aaataacggt
    75841 catgggaaac aatcaataat gcaccagtat agctttgcag ataattctca agccaggaaa
    75901 gtgtctcgat atctaaatgg ttcgttggtt catctagaat caaaatgtca gggtgttgca
    75961 aaagcatgcg agctaaagct aaacgtgtgc gctgtccgcc tgataaagta tcgattttct
    76021 gatcataaaa tgaagaatcg aatttaaacc catgcaatac ggaacgaatt tcattttcat
    76081 aaccatagcc gtttcgatct gaaaaatcgt gttgcagccg gtcgtattct tttaaataag
    76141 aactataagc aggtgtgtct ggcgtacctt cgctaatgaa aatctctagc tcgcgcatcc
    76201 gctcttccat ttgtctaact tgttcaaaag ctttcagcat ctcgttccaa actgtttcat
    76261 tggaatcaag tcctgtgtcc tgagcgaggt atccgagagt cgctgtttta tttttagcga
    76321 tctttccttc atcaggtgct tcaattccag caatgatctt taataaagtc gattttccag
    76381 cgccatttct gccaactaaa gcgacacgag cgcctgtact gatttctaaa tgtatgtttt
    76441 caaataagac ttctgcacca aaatggcgag caatctgatt ggcttgtaat aatatcatgg
    76501 aatcctcttt tctactttgt gaattctcct caaataaaaa actatttttc aattagtagt
    76561 ctaccataaa ttccattata ctgcgagatt tgcagaaatg ataaataaat gaataaagaa
    76621 taaaatttca caatatctga gcaatggaat tgattttttc ctataaatac tgctatgatg
    76681 gagaaatcaa agatgtaatt agacaatacg tggaggtcat ttcgtgaaag atcatattat
    76741 tccaaaggcg acagctaaaa gactgccttt gtattatcgc tatttaagga ttttaaataa
    76801 tgctggaaag aaaaaagtgt cttcaactga attaagtgaa gccgttcaag tagatagtgc
    76861 aacgatccgt cgagattttt cttattttgg cgaattagga aaacgtgggt atggttatga
    76921 tgtagaagat cttatgcact tttttgcaaa gacattaaac gacgatcaat tgacaaatgt
    76981 tgcgctgatc ggtgtaggga acttgggaag tgctttacta aaatacaaat tccatcaaag
    77041 taatagcata cgtatcagtt gtgcttttga tgtgaatgag gaaatcgttg ggcgcatcgt
    77101 agacggtatc cccgtttatc caatggaaga tatgtcagaa caaatcaaga tccaacaaat
    77161 cgaagtagca attttaacga tacccgctga aagagcacaa gaagtagtca atcagttaac
    77221 ggaagcagga atcaaaggaa tcttgaactt taccgctgct cgattgaatg ctccgtcaga
    77281 tgtattgatc caaaatgttg atttgacgaa tgaattgcaa accttgatct atttcttgca
    77341 ttctgacgca aatgcgcatt ccgaaaatac agcagaataa aaataaaaag cgattgaaca
    77401 aatgaaggca aaactttcac ttgttcaatc gcttttttct gtttggacaa cgattatttt
    77461 gttcggaaga tttcttcata agaaagggaa tcatcttctg ctgtcagttc atctagtaga
    77521 taacggattt ttttaaagtc gatgaaccgt gcttgccgaa agacttcttt tccttgatga
    77581 aaaatcagga caactggcgc agtcagcact tcaaatttac tagctacttc tggtgtttca
    77641 aatgcatcca aatgaagtgc cggaatatca taatgtgtga gaagttcctc tagtcgtgga
    77701 cgaacagcgt gacaaacact gcaattaggc atcgaaagat aaatgacggc aacggatgac
    77761 tgttgcagta tgtcttgaac ttcagtcaag ttagtggttt gtttcatttt tattctccct
    77821 tttatgatag aagtgatatg tatgagacct taatttaatg ggaataacag aaaaagtcaa
    77881 ttgaagtgct ttggtaaaaa ataagagttg tgtaaaaatt tacagagcaa cttgtttttt
    77941 ttgaataaaa gcgtatattt gcaaagcgaa tgaactagaa ataaaaagga ggaaactcat
    78001 gttttctttt attccaaatt cggttttgca gatgagtacg attttcttat cgatcgttat
    78061 tgaagcatta cctttcgtat tattaggctg tcttatctca ggcgccttac aagtattttt
    78121 gaccccagaa cgtgtgaaga gatggcttcc tgaaaatcgt atcttctcta tttttactgg
    78181 ttcgatctta ggatttttct ttccttcatg tgaatgcggc atcgtaccga tcgttcatca
    78241 atttgttaaa aaaggcgtac cagttcacac agcgtttgct tttatgctga ctgctccgat
    78301 catcaatcca atcgtgattt tttccacttt tatcgcattt ggcaattctt ggcagatggc
    78361 aagctggcgg gtgataggca gcttttttgt tgcccttgtg attggtgtct ggcttgccta
    78421 ttttcaaaaa gacgctgtat tgaaagaaaa aatcacgtct aaagtagcag aaaatcacca
    78481 tcatcatgtc catgaagaag aacaaagtga gaagaatgaa ccaaaaaatc agctgatcca
    78541 tcaaactggg catgtgttga cacattcaat cgacgaattt tttgatacag gtcgttattt
    78601 gatcattggc ggattaatcg cagctgcgat gcagacttat ttaccaacta gaattatgct
    78661 aacgctcggc tcaacaaaga ttttagccat tatcatcatg ctgttattgg cctttacgat
    78721 gtctctttgc tcagaagcag atgcgtttat aggatcttct ttgttaagtc tgttcggcac
    78781 agcacctgta gctgcctttt tggtctttgg cccaatggtc gatatcaaaa atctactgat
    78841 gatgaaacga tattttacag gacgctttat tttatcgctg atcggactga ttgcccttag
    78901 cgttattgga ttcacattat ttatttagga gggaaaatat gctgcggttt ttgattttat
    78961 ctgggtattt cgaattgatg atgtacttgc aagtttctgg caaactcgat caatatatca
    79021 atgtccatta tcgctactta gctattcttt caatgatttt atctgctctt ttggctttag
    79081 ttcagctgta tttgtgggtc aaaaacggaa aagcagaaga aaaggcacac gagcacgatc
    79141 atgaccatgg gttgtcaaaa ccttatcaac gtggaatcgc ttatgtactg ctagctttgc
    79201 cagtaatcgt ggggacgttg tttccaacag ttagtctcga cactacgatc gtagaagcca
    79261 aaggatttaa tttcccagta agtaaagaat cagtagggga tccggagatg cagacacaat
    79321 acttaaagcc ggataccagt atttatttca acaaatcaga ctacttagac caaatgaatg
    79381 agttgaaaga aaagtatggg gataagcaaa ctatcaagat cacagatgaa aactatttag
    79441 aagttatgga gctaatttat aattacccta gcgaattcat tggcaaaaaa atcagctatg
    79501 aaggatttgt ttaccaaaca ccgaacgata gcaatacaga tatcttcttg ttccgattcg
    79561 gcattatcca ttgtgtggca gattcaggag tctttggttt gttgacgcat atgccggaag
    79621 gagtaacggt taaaaatgat gaatggtata aaatagaagg aaccatccaa tcagactatt
    79681 atcaaccatt caagcgtgaa atcccatctg tagttgttac aaacgctgag aaagtctctg
    79741 ctccgaaaaa tcagtatgtg tatcgttctt tttaaaagaa caaaataaat aaacatagct
    79801 cagacagctg acacgcaagg acgtcggttg tctggctttt tttgcatgat gtattcattt
    79861 gtttgatatt tattttagaa gcagcatcct gaaagtattt gatatcggtt tcaaaggtgt
    79921 tggaaaagat tgtgaagaga attacaattt attcctaaca tgtatgttga aaagttgtat
    79981 gaaatgattt tgatagtaaa atatcgagtg gaggtacttt ttatggtaac gcgtatattt
    80041 attttggatg atagtttggt ttttgaaaaa atgctagtag aaattttgga agaatccaga
    80101 attttattaa gtccttggaa atttgagatc gttcagggat tgaacgttat acaatttgtt
    80161 gaattcatta aaaataatga aatctattcc tcagatattt ttttcctaga catcgatttg
    80221 aacgcctatt atagcggtat cgaactaggc gaaatgctta gagaaaagaa tgaaaagtgt
    80281 aaaattgttt atctaacgtc tcatccgaat aaagcaataa ctgttattaa tcgaaagatt
    80341 acgcctagtg gttatttgat aaaagaattc acttatgaaa aaaataaaaa aagcatccaa
    80401 agttacttat tgaaagaaaa agaaaactat cctgttcaac atgctacgaa aaaaaggtgg
    80461 acaactgtcc gttatggtaa tagcgatcac tatatccatt acgatgaaat cctttatcta
    80521 atgagcatgc caggttataa aaataaaata ggcctagtat tgaaagatga agagattctc
    80581 gtaaacggca caattaaaaa atataaaaaa tcactcgaat atgagtattt atgtaaagat
    80641 ttaaaagctt atatcattaa tactgaaaac attacatcta ttagcagagt aaacgaaata
    80701 gtaacatttg caaatggaga acaactagag atgggaaaga gaagtattga taaactaaag
    80761 aattttatgc aagaaaagat agaaaatgaa tcattttaaa gaaaacaaag actcatatct
    80821 tacctgttag aagagtgttt tcctgtagaa gagaacaacc caagtagcag cctagtcgtt
    80881 tctatccttt tttgttatgc actctttttg acatttactc caccaataac gaagggtaca
    80941 ataaaaagta ataacaaaca aaaggagtac agaaatgatt gaattcatca atgtaagtaa
    81001 aagttacggg aaaaaacaag cattaaaaga cttgtctctc tcgataagac aaggggaaat
    81061 ttttggattt ttaggacata atggtgccgg aaaatcaact acgattaaaa gtttagttag
    81121 tattattgaa ccatcatcgg gaacgatttt agtagatgga atgaaattga cggaaaatcg
    81181 attgagtatc aaacaaaaaa tcggttatgt gcctgattcc ccagatattt ttttacaatt
    81241 gacggctgga gaatattggg acttgatttc agctgcctat gaattagaca cacaaaaaaa
    81301 agaaaagcgt ttagcagaat tgacagcgct ttttgatatg tatagtcatc aaaatgaaac
    81361 aatcgcaagt ttttcacatg gaatgcgtca aaaaacgatt ttgatcggca cgttgctgcc
    81421 agatccagat atttgggtat tggatgaacc tttgcaagga cttgatcctc aagcagcatt
    81481 tgatttgaaa gaaatgatga aggctcatgc cgcaaaagga aaaacagtca ttttttctac
    81541 acatgtattg gataccgcac aacagttatg cgataaactg gctattttga agaaaggtga
    81601 attgatttat cagggggcag ttagtgaatt attgaatggc tcacctgatg aaacgctaga
    81661 aaagatttat ttgaaaatgg ctggaaggca agcgacagga gatggaccgt atgtataagc
    81721 gacaattatt agaattaatt cgagttaatc ttttatatgt taatccacag ctcacgaata
    81781 aagcaagaga gaaaggaaaa aagggaaagc gattaactat ctatcttttg tctcaaaatc
    81841 ttttttcagg gctgattttt ttactgattt acggatttgc tatgtttgct atgaactttg
    81901 ttaaattgcc aggtttcttt acttattacg tggcactttt tggtatgtta ggtttaactc
    81961 aaagtatatc agtcatttat aatatctttt ttgaaggaaa tgacctgcaa gtatttttgc
    82021 ctctgccttt tggacaagaa caggtttttt cagcaaagat tttggtagtt gctttgacca
    82081 tcattccttt tgtttttccg cttctagtca tgtttatttt aaccggatgg cgagctgatc
    82141 tagggatttt gcttactatc attatctcaa tccttttgtt ttctctattt ttgattattc
    82201 ttttttgtct ggctagtttg attgtttttg gtttggctcg aacagcagtg tttaaaaaac
    82261 atagaaaaac agtttctagt atcttgcttg gcagctcgat gctgatcgca tttgctggaa
    82321 tcatgtggat gaatcatcaa acggggacta ttgaaaatga gttaacagat cggcatccga
    82381 tttcgatatt attgccattc tatgatgctg ccagtcagcc actttctcaa caaggtttat
    82441 tgggttgggg aatactagct atcacggctc tgggattttt ccttgctgta aaacagctga
    82501 tagtaccaaa gcttacagag caactgactg attcaaacag tgagttcaaa ataaaaagaa
    82561 cccataaaaa gaaccaaaat gtgcatcagc ttttatttag ttacaatgct caattagtaa
    82621 aagaacctaa tttgattatg caagttcttt cttcttcctt gttaactcca atcattttta
    82681 ttcttgcttt tgccctagga ggcgaaataa aattaactga tttagataat aagttaatcg
    82741 gtgttgtttt cctggtaggg atcgcattgg ctttcttaac agttaatcag acttctttta
    82801 tcagcaatct tatttctttg gatcaagaaa attttctgtt tatccgttct ttgccaatct
    82861 ctatgaacca atatttaaaa gaaaagttca gattcggatg gatattgcag agttttttaa
    82921 ctggtgggat cgcattatta agcggactgt cttttcagct gcctttgttt tttatcccta
    82981 gcttgctatt tggttcttta ttcggctgct acttattgtc actacgttat ttcgctagag
    83041 attatcggtt gctgctgctg aactggacca atattaacca attatttaac agaggttcag
    83101 gaagtttggg tttagtcgca actatgatgg gttcattgat catcagtatt attcttatcg
    83161 tcttatacgg aatgttggct ttgttttatc cattttggtt agtgaatatt cctgtgatcg
    83221 gtttagttct aatactttcc gctctatgga tcgtttatta tcaacgaacg ttttggaaaa
    83281 aatttgaata aagaagaggg gccgtgacaa aaatcatgtc gcagcccctt ttttacagat
    83341 agagggtgtt caaaagtcag tttctcagta ttaagtcaat tttatgtaaa aatgtgagaa
    83401 gctattttta caaaactttt tttaatactc ataattactt cttattgctc ctgcggttac
    83461 ttgtcgcaat gtttgtgaga tattttgtca tcgctcaagg ctgaacgtct ctttcacaac
    83521 cttttctttg ttacacttcg tttatttaaa aagccgtttt tttcgtttca tcatgattgc
    83581 gtcacgcttt actttgaaat tacggatgtt atggttaaag acttctgata atacaagacc
    83641 taaagcgatt gctccagcaa tcataattgc ttggaccgca taatgagccg cttctcgata
    83701 ggattcagta acgagatttc gaaccgcttg ataagctaaa ccaccaggga ctagaggaac
    83761 catcccggga atattgaaaa tcgttacggg cattttcaaa cgtttagaga aaagatcgct
    83821 gatgaatgct actcctaaag aaccaagcaa tgaaccaaaa gcagcagatc cgcctatttg
    83881 aacacagatc cagtagatca tccagccaaa agcaccagaa agaccacaac aaatcaatga
    83941 acgcctaggt acatttgtaa taacggcaaa tgccgcagtc gctaaaaaac taaaagaaaa
    84001 ctgaataaat atcgtcccca tacttgtgtt cctttcctaa taaaaaagct ggaaaataaa
    84061 agcaatcgca aaaccgatca tcgtagcagt catcatcgct tctgttccgc gagaaactcc
    84121 tgagacataa tgtccagcta gcaaatcacg gatagcgttc gtaatttgaa ctccaggaac
    84181 taaaggcatc acacatccga taatgattag atcctgattg cttcctaagc caaaacgtac
    84241 gctgtagata gcggctatgc caatcaataa agaagataaa aattccgata aaaatttgat
    84301 tctaaaaaat ttcaaactat atagataagc tgcgtatccc aatcctccaa tgactaaagt
    84361 cacaggtaaa tcggtcatct ttccgccaaa taacaccata attgttccac tgatcactgc
    84421 cgcgctaacg atttgcaacc agacaggaaa gaaagaacgg tctcggtcga ttgcttgcag
    84481 ttcgcagtaa agttcttcta atgttagctc tgaagcaaca tatttacgtg aaagatcatt
    84541 tacttttact actttttcta aattaatcga acggttggta atttgttcca ttcggatggt
    84601 cgaggtacga tcaagaccaa taaagagacc ggtttgagtg acatagctga ccaaacggaa
    84661 atttccagaa gctaaagcaa ttcgactcat cgtatcttct acccgataca tctccgcatt
    84721 gctttccatc atgattttcc cagcgagtaa acaagtgtcc agcaacagat ctaattcttt
    84781 tcccgtcata gcatcttctc caataattga ttgattagtt ttattttact acaaaacata
    84841 ccagaagcgt aaagaataaa aaaacttgag gaaggaataa aagaggcgta tactgttttt
    84901 gtattgcggg tgtagtttag tggtaaaatc ccagcttccc aagctgatgt cgcgggttcg
    84961 attcccgtca cccgcttaaa ataggttgtg aaaaagctgc tctgcaacaa gtaataagaa
    85021 cagccaagca aaaacaactt cttatgtttt taacttggat ggacttatta ccgcagatgc
    85081 aagcttttgc gcaccgttta ccaagcggtt gtgaaatcag tgtattgtct tgagtatgaa
    85141 gaaaattttt gaataataat aaatacctta cagatagagt ggtgactgat tagagacgag
    85201 gaatccgtct cttttttgta tttttaagcc atcttaaaac aggttttatt ttttgctgtt
    85261 tctatttctt tctagtattt atttataggg gaacatatgt taagatttag ggagctgtta
    85321 gtaaaggagt aaagaaatga gcaaaaaaac aaccaaggcg atcgtgggga tcgtcgctgc
    85381 tgtgattgca ggggcattcg gtgtttccac cacacaaaaa gattcagata tctttcagca
    85441 gattacaact atttttgatt ccacaccaca agcttctcaa gtagcggatg tgccagaata
    85501 tgatggaaaa catcaagaaa tagaaatcaa tgggaatcag ccggaattta caaaagagga
    85561 tcttagcctt tccaaaggaa catgggaaag ctattcaaat attgataaat tgaacagagt
    85621 aggacaagcc aatgctatgc ttggaaaaga tttgttccct actgaaaaga gagagccttt
    85681 gtatattgac ccgacaggtt ggaaacaaaa gaaattaagt gacgggcaat ggttatataa
    85741 tcgcagccat ttaattggtt tccagctgac tggtcaaaat aacaacatta aaaacttgat
    85801 gacagggaca cgttcgttga atgctccata catgcttgct cacgaaaatg atattgcggc
    85861 atatatcaaa gaaacaaatc atcatgtacg ctaccgtgtc actcctcatt ttgaggggaa
    85921 tgaactggta gccaggggag tgcaattgga agcagaaagc attgaagaca aaaaaattga
    85981 attcaatgtc tttatctaca atgttcaaga tggctacacg atcaattatg agacaggaca
    86041 agcaacaaaa acaaaaaatt agtagcagct attcgaattt tttatgaata ttatgaaaaa
    86101 attgtgtaaa aacatacatt caaagaaata tagcgtatac tttatgaaaa gagtactttt
    86161 gataaagcta cagaaaaaag gaaaaaaaca tacaaatagt gtttcttaat ttattcggaa
    86221 gtttggaata tttgtttgta gttttccctt tcttcgagta aaataaggtt gtagaaggta
    86281 agctggaatt accttgcggg aaagaggtgt agaatcatgg gttttacaga tgaaaccgta
    86341 cgttttggtt ttgacgatag tcgcaaaaaa gaagtgagcg aaacattagc gattgtttat
    86401 cgggctttgg aagaaaaagg ctacaatcca atcaatcaga ttgtcggcta cttgctttct
    86461 ggagacccgg cctacattcc tcgttatcgt gatgctcgaa atttgattcg ccgtcacgaa
    86521 cgcgatgaga tcatggaagt aattgtcaag gactacctct caaatcatgg agtgaattta
    86581 tgagagtaat gggcttggat gtcggttcta agacagtagg tgttgctatc agtgacccct
    86641 ttggctggac agctcaagga gtagagatca ttcggatcaa tgaagcagaa gaagagtttg
    86701 gctttgatcg gttgaaggaa ttagtagcac aatatgaagt agaccggttt gtagttggat
    86761 tacctaaaaa tatgaacaat tccatcggac caagggcaga agcttccatg tcatacggag
    86821 aaaagatcaa agaactattc tccttgccag ttgattatca agatgaacga ttaaccactg
    86881 ttcaggcaga acgtatgcta gtagagcagg caaatacttc aagagccaaa cgaaaaaaag
    86941 tcatcgataa attagcagct gtgatgatct tacaaaatta tttagacggc tcaggtaagc
    87001 aattatttta ataataaaag aaaagaggta atcttatgac agaacataac cattcccacg
    87061 accatgaagg acacgaacac atcacattgg tggatgacca aggaaatgaa acattatatg
    87121 aaatcctact aacagttgac ggacaagaag aatttggacg taactatgtt cttttatatc
    87181 ctgctggtgt ttcagaagat gaagatgtag aattacaagc ttatgcatac atcgaaaacg
    87241 aagatggaac agaaggcgag ttagaacaaa tcgaaacaga cgcagaatgg gacatgattg
    87301 aagaagtatt caatacattc atggcggaag aagaagaata gactataaag ctaggagaaa
    87361 ccttgatatg atagggtttc tccttttttt attttttact gtatgccata ttgtatgcca
    87421 tctagtcaat atttaagtgt tgtactaatt tttcaacagt cttttctttc gtttcttttg
    87481 ccaagtgacc gtaacaatcg atcgtctgac taatttttgt atgccgtaat ctagtttgaa
    87541 cttctaccat agtagctcca gcgtccaaga gcattgaagc atgagtgtgt cttgctacat
    87601 gcacaccgag aaatggaacc atagcttttt cacaaatagt aatcaatctt gcataaatag
    87661 cgtggtgtgt gattagttct ttatagatac cacaaaaaat catagtcgga tcagttacac
    87721 caagtgtcag catatatttc cgctgattaa tctgccattt ttttaatata gaaattgttc
    87781 tttcatctag tggaagaacc ccagcactac aatcagtttt aggatcactg attttatagc
    87841 catttttgga ttggcttagt gttttattga tggtgaccgt attttcttga aaatcaatgt
    87901 ctgaccaatt caatgcaagg gcttcactga tcctaacccc gctataaaag agaaagcgta
    87961 gtaacgtttt atcaaagtcg tttcgataag tttcaggttt gctgtttagg taatcaaaca
    88021 aggtgatcag ttgttcttta ctgtaaactt tgaccttttc tttatgctta gtggtggttc
    88081 ttttaggcat taatacgttg tctagtggat tagtctcaat aatcccaaga ttgatagcgt
    88141 agctaaaaat acgcttagaa gttgaatgaa gtaccttgta ttggtcagta gtttcagccc
    88201 agctatttac aatttgttgg gcttttttta ccgtgacttc attcacacga agatttccat
    88261 attttggaag aatccacttt ctaaacttga tttcagtttt catgaaggtg gcttctcgga
    88321 cagtggtttt atatgtttct agccacaagt cataaacttc tttaaacgtt tcgttgttcg
    88381 cttttttaat accgttttta tcgtagtcca cttgcaagcg tgacaaggca agctgggctt
    88441 ctttttttgt tttgtaacct cgtctacgaa ccttatcagg tttgccagtc attttgttca
    88501 ttccaagata ggcttcatag agccaaaatt tacctttttt agtttcatat tgcttgaatg
    88561 ttgccataat agttttccct ccatagtgta gcaagccgtg ggggcggttg gaggaggcag
    88621 tttattatta ttttgaatag tgtttattta gctcagtaac aagagctgtg aactctttta
    88681 gaagatcatt ttcataaaat tcttgatact cttttttctc atctgtattt ttttctgaat
    88741 cattaattaa tgagtcatat cgtaaaagcg tatttagaag agctgcgaga taagcaattg
    88801 gggtactccc gtcaggaaat tcaattcgag atcctgtttt aagatatttt attgcgtttg
    88861 ttaaataaat cttctgtgag acagtcaggt tatcttcttc gatgttattg atagcattag
    88921 aaagattaga atcaattata gagttatttt tttcaatagt ttttttcaat tgcttttcaa
    88981 gcacatttcg cttaatttcg ggcatcatat gcatatctct taatgtataa gatccgttta
    89041 atagataggt agtggataca tctaatacct ttgaaagttg tttaagcctg gtgttattgg
    89101 gaagattata gccattttcc caattactaa cggcaccttt actggccggt ggggataatt
    89161 tacctccaag ttgttccatt gagagtccct tttcaattct agccagtcta attctttgtc
    89221 ctgtctccac tgcttcgggg tatttttttc tagccacatt ttcaccacca ttcatatatt
    89281 ttctctacta tatcacgatt taaatatgtg tgaaacataa aagttcaaaa aagtatgtta
    89341 aaattcttgc aaatgaaaag gtatgatggt atattgagtt tataagaggt atagaaatgt
    89401 attcaagagc ctcttgcatt tttttaaaat ctttgtatag aaatgtatgc taacgagggg
    89461 ggataaatat ggaattaaaa attgacaaac tcacgcttga tgatccagct tttaaaatta
    89521 ttgacgatcg agttgttcaa caagtagagc aaactcaagg aacgttactt tcattattag
    89581 aaaaaaagta ccagtttcca aaatatatga acaaggcaca agctgctaag tatatgaacg
    89641 tttcatacaa tactatgatg cagaaatatg ttcctaacgg tttaaagttg attatagttg
    89701 atggagtcgt aagaatttca caagatgaat gtgatcgctt tatggaagaa taccaaaaat
    89761 aaacgcaagc cgtgggggca aagggattaa taggaggaaa taataatgga atcgacacaa
    89821 aataaagcaa tcgaaaaagt attatcaaca attcttacag aagacacagc ggcagaatta
    89881 gcaaacttgg aaggcaaagc gctagaagaa acgtttgaat gtttgtacga acaaatggac
    89941 tatcaaaaat tgctaccaca agaaccaacg gtaagcggag tattgcgtgg cttgaacgat
    90001 ttagtacaag cagagtttaa agaacgactt tctattgaag agtatcaaga aattttgtat
    90061 cagcaagttg agttgttagc gagcttgcta gggcttgaac tggaggacgt tgaatgaaaa
    90121 ctgaaatttg gaacggacac attataagat ttgtcgatat taatgatgaa tggtgggcag
    90181 tggcgaaaga tgttgcggaa gcgttaggtc ttaaacaggt tactagagcc attcactctt
    90241 tgcctaaaga tggggttact actagtaagg tcatcgattc attgggcaga acacaagatg
    90301 taaacatcat caacgaaaaa aaatatttac cgcatggcat tcaaaagccg taaaaaagaa
    90361 gccgaggcat ttcaagactg gattttcgac atcatcaaag aactacgcca gtccacagga
    90421 cttgaaggct ttcaagtatt ccgcatgcta gacaaagaac accaaaaaga agcaatgact
    90481 aaattaagcc acgctattac tgaaccaaaa ccagtagact acattaaagc gaatgtgata
    90541 gccaataaag ctgtatcaac tatttatggt cattccaaaa tggtaaaaaa gaaagatatg
    90601 actcctgaaa tgttggttga tcgtgaacct attttagatg agacagtaga attaatgact
    90661 gttaaagaaa aatatgggtt gcagttttca gtgagtgaga aaatttataa tcgttctgct
    90721 gaattgcaga caacttagga gcattaacat gatcgagaac aaagaaaaag ccttatccat
    90781 aacagtcttg gcggacagga taaaggcaac ttaattggga taaaacccga tattctttgt
    90841 tccgattata ccatattaaa aaatcggagg gaaccattat gaaacgaaac ttaattatag
    90901 gaagcatttt aatcggcgga attttcggct tattaccatt aattgcaaaa atagctatct
    90961 ttgcaggatt agcgttatat ctcttcactg cttatgacga gtacgactat caattaagaa
    91021 ctggagggca taagtagatg tacaaaacaa aactattaaa ccaactcgat agccttgaat
    91081 tagaggaaat taatcaaggg atcgctgaac tagaaaataa tatcgggaaa acctactttg
    91141 gaaattcatt caatgaaaaa ctaacagttt tgtatgtact caaaaaacat gcagaacaca
    91201 aaataatttg tcgagaaatc aacgagctaa aaaatcagat tttaaccgca tggttaaaca
    91261 ttactgatat gcaagaagca agggtgaaaa catttaatac atgggtaaag tatcagaatc
    91321 aattaaaagg tgctgaattt gttcgggatg ggttgaaata cgaacttgag cagttgaagc
    91381 tcatggaggt gtctgaatga tttataccag tattttgaaa aaggaggaga ggtttattgg
    91441 caaataatta tcaagcagca acggaattac aaaagtcggg atggtcaatt tatcccttat
    91501 ctcctggaac taaaattcca ataaaaggct ctagtggttg gaatgatgca actaacgatt
    91561 ctaatcaaat taatgaatgg tggacggaag atgcaactag aaatattggg ttaatgcttg
    91621 aagatcatca aatggtggta gttgacctag atcaacattc tgaaacacga aacggcgttg
    91681 agaattataa aaaacttgct tcaatgtatg atccatttcc ttctacttat acggaaatca
    91741 caccaggtaa aggaatccat ttcttcttta aaattcctga tggtgtaatt atccaacaag
    91801 aaacaggtgc atttgctgat atcttcgggc gtgatgataa aggtaaaagt ttaagcggga
    91861 ttgatattat cactaaagga gttccaatag cgccaactat gattatttca agaagtgttg
    91921 ggagagcata tgaacctatt gaaaacgtat ctctaaataa tattgcacca gctcccgatt
    91981 ggctagtgaa cctactgcta aaaaaaccgc aagaaataaa gactcaatac cgacccgtag
    92041 caaaaacagg gacagcaaaa aaattagata tgattgttca aggcgctggc gaaggtagta
    92101 gaaacgatca tttaactaag ttatgcggtt ggttactatg gcacggtgta gataatcaaa
    92161 ctttagcaga attgatttat acagcaaatg cgtacaatga accaccgcta aaagataaag
    92221 aagtgaatca aattattcga tcgatgatta aaaaagattt gagggggaaa gctcatggct
    92281 cataagcata aattagatag tattttagac tttcccgaag ccagcgagcg tgaagacaat
    92341 atcattgaat tgaaaacctg gatgtcacga ttaagatgca ataaagacga tcagattaaa
    92401 agtaacagtg ttgtgaatgc agaattgatt ttaaccaatg atagcaattt agctggaaca
    92461 attgcttata acgaatttag cggatacatt cacctattaa aagattctcc ttggatcaat
    92521 cgtaccgctg gagaatggga agacagtttt gaggatgcct taacggcata tattgaagaa
    92581 aactacaatg tagtttttga tgacaacaaa atacacaaag cagtagtaaa tgtggctaga
    92641 aagaatgttt tcaacccagt aaaagaacgt atcgaaaaag ttaaatggga tcaacagcct
    92701 agactggaaa cactgtttat tgacctttta ggggtagacg acaaccttta cacacgagaa
    92761 gtaacaaaac gttggatagt cggatcagtc gcacgtatct ataaaccagg aataaagttt
    92821 gagatcgttc cagttttgga tggaccgcaa gggattggta agagtaccgt tccagcatta
    92881 ctttacacgg atgatttttt caccgattca ttagattcgc taggtgaaaa gaaagatgat
    92941 tatatgcagc ttcaaggcaa tgtaattatt gaacttggag aattatcttc aatgaacaaa
    93001 actaaaattg aacagataaa aaacttcatc agtgccaaga tcgataaaat tagacctcct
    93061 tatggtcgga atgtcatagc ttgggcaagg caatgtgtat ttattggaac gtcgaatgat
    93121 gggcagtatt tgaaagatga tacaggaaac cgccgttttt atcctttgcc ttgtaagaat
    93181 aagccaaaga tgaatccgtt taaaacaaag gatgaatatt ttcttcaagt gttagctgaa
    93241 gcaaaggtgc tttttgataa aggacaacga atctatttta gtcccgatga agataaagaa
    93301 gtcttagaaa ttgcaaaaga ctatcaagag gatgccaaga tggagaatcc aattaaagaa
    93361 gcaatcacta aatatttgga aatggaaatc ccgtataaat gggaagaagc tccagcgtgg
    93421 gcgagacgtt cttattatca gaactatcct gacagtaaat gtaatgagaa aaatttacag
    93481 catttctata atggagctac tatgcaacaa gagttttatt tagttgatag cgtactcacc
    93541 gctgatattt tggaagctgt ttttgataaa caagcaaagg atttattgaa tggtcgatca
    93601 gatgcagaaa ctaaaaagat tgccttgatt attagcagta ttcctggttg ggaacggaag
    93661 caactgccaa aaagaaataa acgacgagga ttttacaata agtcaaatgc ctatgaaaat
    93721 aaaaatcgag ctggaaagta caaaaagtaa cgggtagtaa cgggtcagta acacctagcc
    93781 cgttactagt ttacccttac tgccccaagg gatacagcaa tttggtaacg gggtaacacc
    93841 ttatttcctt aaaacttttg aaatagttag ctaatatgct ggggaataat gttactggat
    93901 tttacccgtt acctgttacc aaaacgctag aacccttgtc cccgttggga ttgagtggta
    93961 acgggtacct gttacgtagg tgttaccacg tgttacgaat caaggaggaa agtaaatgct
    94021 caatattatt tcaacaaaca aagcaccgaa ctttcaatat acagatgaaa tggatcggtt
    94081 tctaatgaac acgctggcat ttagtgtggg attggtaaca gaagattatt ccacatttga
    94141 tccagaagta ttaaagataa tggaagaaga acctgattgg ctgcaggagt cagtagcgtg
    94201 gtgtcaatcg ctagtagtgg gatcattggt agatagtggc aactatgatg atacaggtga
    94261 gctaatggat gaatttaatt gtctgcttaa cctatatgat cgagcgagac aacgagagct
    94321 tacatcgaat gaggataatt tgtttttaaa catccatgat aagttcttgg ctttgctact
    94381 aacagatgat gaattgataa ctaatttatt ggaggtagaa taattatgca tatggaaagt
    94441 attagatact tcgatggtga gaaacacatt gagtattcaa acactcaaga agatgtagac
    94501 tttattagtt ttgaggaaga taaaatcact aaagttaatt ttaaagacgg aacgtgcttg
    94561 aagatagttt cgccttatat tgaatacaaa tcaaaatggg aacaagatga ttctgacgaa
    94621 cgagatcgct ttggttggta atctatggca gttaaaaaac aatgtaatca tgctggatgt
    94681 aaaacattga ttgattatag gcagaagtat tgtgggaagc ataaagcaaa gcaaacggca
    94741 attaagcgtg aagaaagaaa gcaaagcgaa ggtaaatatt ttcaattcta tcaaagtaga
    94801 acatggcgaa aggcttcata cttgtatcgg ttaaatcatc cagtatgtga ggattgccta
    94861 gaagaaggct tgataaggaa agctgatgtt gtagatcaca aaattgaact aaaagatgat
    94921 tggaatagac ggttagatga aactaatttt cgttctttat gccatgcaca ccataattcc
    94981 aagacagcca atgaaagaca aagacgagaa aagagtaccc tttgagttaa gggggcatag
    95041 tgtgaaactt actgacaatc gatgcctact cattttggta caaataaccg ttgtgaaaag
    95101 gcataagggt aattacaaat aacgattaca cttgtaatca caagtgaaaa agtatataat
    95161 gaaagtagga gatttattga aaaatacaat tgttattact gataaaacag ggaaacagcg
    95221 caccattgag ttacctcatt ttggtcaagt gaccattcaa atgcagaacg gaaaaatcat
    95281 ttatatagat aaattagata aagaaaaatt ctgatcgaaa aacgaaggaa tgtgagctta
    95341 attgcttacg ttcctttttc ttttgtctga aaggaggaca acatgggaca ccctaaaata
    95401 ttacacgata caaaaggcaa tatatcaagt gaagaaaaag caatccgtgt ggacgctaga
    95461 gaagaattgt tcaagcagca accattaata aatatcacgc cccctgactg gatggcagcg
    95521 agcgctagaa gtgaatggaa tcgtatcgta ccaacattaa aaaaagatta tccattgagt
    95581 gaagcagatt atggttcatt ggtagcctat tgtttagcct ttgctcgaat gaaaacagcc
    95641 gaagccgaga taagaaaatc aggtacgttt atcacatgcg aaaacggagt aaagaaagcc
    95701 aatccagcgg ttcgggttca atcacaagct atgagtgatt tgaaaaaaca agccacctca
    95761 ctaggtatga ccttagaatc acgatcaaaa ctagctttga acaaggttaa aaatgatgaa
    95821 cccgaagacc cgttcaaaga gttgatgaat tcatgaatga ttacattgag aaagtcctat
    95881 caggtaagct gattgcaccc aagaaaatta tccaagcgtg tgagcgtcat ataagcgatt
    95941 tggagcgttc taagtcagat agcttccctt atgtgttcga tgaagaacaa gccaccaaag
    96001 cgattaaatt cattgagttg ctaccatcta cggacggtaa agcaatcaag atgttaggat
    96061 ttcaaaaatt cattctaggc agtctttatg gctggcgtac taaagaaggg aattacaggc
    96121 gattcaatcg agcgtttacc agtatgagcc gtaagaatgg gaaaacgtat atcgcaagtg
    96181 gcatggctgc caatgcgttg attatggaac aagaaccagc agaagcaagg caagtattgt
    96241 ttgtaagtaa cgctttgaaa caagctaaat tgggctatga tatgctgtct aattcactta
    96301 gaaacgtggt caagtctagt aagtttttaa gaccacaact gaaaattatg aactctaaga
    96361 ttcagcactt gccctctaat tcgttcgcta tggcactggc tagtgaaacc agcacgctag
    96421 atgggtttgc accaacaacc gcaattcttg acgagtggca cgaagcaaaa actcgtaaaa
    96481 cgtacaacgt cattaagtca ggaatgaccc aacaaaagaa cggcttattg tgtgttatta
    96541 gtaccgctgg gcttgattta aacgttccta tgtacgagga atacttattg ttagatcgtg
    96601 tgctaaaagg cgaagaacaa gccgacaggt actttatagc gatatgggaa ttggacgatc
    96661 ccgaagaaat tcacgatcaa gagaaatgga tcaaagccaa tccgattttt gaaagtgaag
    96721 aaatcaaaaa agtaatgatt ccaaccattc aagatgatgt gaaccttgct ttgaaacaaa
    96781 ataaccttaa ttctgtattg gtgaaaaact tcaatttatg gagacaagcg agtgaggaca
    96841 gctatatgat tgctgaagac tggcaggcaa ccgaagtaga accacaagat attacaggca
    96901 agcccgttta tatcggagtg gatttatcta aaacagatga tttaactagc gtttcatgga
    96961 tcgtaccgct agataacggc gaactttatt gtgattcaca tagctttgta gccaccaaat
    97021 atgggcttca agacaaagaa aagcgcgacg gtttgcctta tcgagaacta gaaaaagctg
    97081 gtgagtgttc cattactcaa ttagaaagtg gaatcgtgga ctatgaccaa gtatttcagt
    97141 ttattcaaga tttgattcaa gaaaatgatt tggaatgtat ggggatttgt tatgacccgt
    97201 ataacgctaa ttcgcttatc agtaaagctg aaaaagccaa ctacccaatg ttagaagtaa
    97261 gacaaggaac gattactcta aacgttccga cccgaacttt tagagaacaa gtttatgaag
    97321 gcaacgttat tcacaataaa aatacgattc tcacccatgc agtgaacaac gctattttaa
    97381 aaacggataa caacggcatt cagattaata aatcaaagaa cagtaacaaa attgatccaa
    97441 tagctgcatt aatcaatgcc tatgtgtttg caatggatta cttcaccaca acggaaggag
    97501 cgaaagcaga caatgaattt tatacaagtg aagaattttc tttctaatta cattcatacc
    97561 gttcttttac ttcttggatt ggtgtgtgtg ttggttgcta tcaccttact aacaaatgtc
    97621 tactatggct tgttagcgct gggcatagtg cttattgggg tagcggtcat gctaaataca
    97681 gaacagaagg gaggttaaaa gatggcattt tttaaagcga gacaaaatac aacgggagat
    97741 cctttcctac aacatgtggt atcaatccaa tcggatgatt acaccaccag ctttacaagc
    97801 gttcgtgcat taagaaatag tgatgtgttt gcagccgttc gaattattgc tagtgatatt
    97861 gcttcaagtc cgattcaatt ggtcaaaaac aatatgccac aagctgataa tgaactggtg
    97921 aagttactaa acgagaaacc taattcagaa atggatggtt ggcatttaaa gtttgctttg
    97981 gcggtcaata tgttgctaaa cggtaatagc tttgcagaga ttaaacgcaa cggtgaaaag
    98041 gtagaagaaa ttcacttatt acctaactca agtgtaacgg ttactcaatt agataatggt
    98101 acgttgtctt atcagattgg tgataaaaaa agacgtgtga agtctagcga tattttgcac
    98161 ttcaaatatt tcacccaaga tggtttaaca ggattaccac cactttatgc gttacgtgat
    98221 gaactgaaaa ttcaacaagc tggcaaccgc acattgcaca atttctttac tcgtggtgtc
    98281 agtgggtcag gtattttgaa ggttcataag tcagatttag acggatcagc aaagaacgca
    98341 attcgtgaaa agtttgaaga agccaacggt tcaagtagtg gagataatgc tcttagaacg
    98401 atcattcttg atgaaacaat ggactacaaa acattagaaa taaacaccga tgttttgaag
    98461 cttatcaatt ccaatgattg gaacacaaaa caaattgcca aggcgtttgg tgtaccgatt
    98521 gagcgtttag gcgttgaaaa tgaacattct agcaccgttc aaagtaatct ccagtatatc
    98581 caaagtacgc tgattcatta ctttaatgtg tttgtgagtg aattagatac gaaacttgaa
    98641 accaatatcc gctttaattc cgatcagtta ctagagactg atcctgaaac aaaagtaaaa
    98701 aatgtattgg atcaggtcaa agggtcactt ctcacgatta atgaggggcg gtcgaaaatg
    98761 gggctacccc ccatggatgg tggcgatcgt ttactagcaa gtttgaactt tacgtattta
    98821 gatacgttgg agaaatatca attaaaagaa caggaaggag ttacaccagt tgaataatga
    98881 agaagaaaag gaaaaacggc tgacagaaga agctgagcta acagccgatt ctcccaaagt
    98941 agagaaagaa aatgaaaaac aaccaacaga cggcaaaact atttcaggct atgcgttgaa
    99001 attcgggcaa ccgtcaaaag atttaggcgg ctttgtagaa gtgattacac ccgaagcatt
    99061 aaaagaggtg gatttatcaa atgtgttctt attatatgga cacgattatt caaagccact
    99121 agctagtgtc aaagcaggta cgttgaaatt gaatgtcgat gatacaggat tacattttga
    99181 agcagagcta accgacacga cctattcaaa tgatgtgtat gagaatatct caaaaggggt
    99241 tgttgattct atgtcctttg ggttcgtgct tggattagat tcattcgaca aaaaagaaga
    99301 tggcacaatt gaacgatcaa tagataaaat caaagcactt aatgaaatta gtgtcgtgac
    99361 cgttcccgct tatgattcat caaatgtcca agtaaataag cgttcttacg aatcgtttat
    99421 gagtaacaac caagcaaaac aaacaaacaa tagcttagaa tccacttcta aagcacaaaa
    99481 ggagagtaat aacatggaaa aaactttaat cgataacgaa aaaacagaat tgcgtgggta
    99541 tgaagaatat atccgttcac aaggcgaagt gcgtgatgga gtcactactg taaatgcagc
    99601 ggcagttgtt cccgaagaag taatcggtga agtctttgat ttgaaacgtt caaattataa
    99661 cttagctcaa tatgcaacag taaaaacagt atcaaatgga caaggtaaat atccagtagc
    99721 aactaaccaa caagcagtgt tagcaacaaa agctgaactt gctgaaattg gtgatattga
    99781 cgctgaaatg tttacttcag ttgattataa agtagaaact cgtgctggta agattgcctt
    99841 atcaaatgag gttgtggaag attcagcagt gaatattgta caagaggtca aagatcagtt
    99901 agcgaaattg gtagaaaaca ctgacaataa gcatatcatg gatttattaa aaacattcac
    99961 taagaaaacg gctgctacgt tggacgattt gaaacaacta tacaatgtgg cattagaccc
   100021 agcattaaat aaaatggtaa ttctaaacca aagcggatat taccacctag atacattgaa
   100081 agattcagat ggacgttaca ttttacaacc cgatgtgaca gcacctagtg gtaaatcatt
   100141 attcggtatg ccagtagtat tgattgcaga tacattgttt gccaatccta aagctggaac
   100201 attccctatg attatggggg atattgcaca atctatcttt gttgctcgta gaaatcaagt
   100261 aacgactcaa tgggaaaaat tcgattacta ctcacaagga cttgcagtga tcgttcgcaa
   100321 cgattataag aaaattgatc aaaatgcttc agtgtatatt gagtttacgc cagttgtaac
   100381 accaaaagta tagaaaaata ttgggcggcg gtttatccca ctgccttttt ttcattagga
   100441 gagataaaat ggtaattttg gatagcataa aaaaaagtat gcggatcgat cacactattg
   100501 atgatgactt tattcaacaa ttgattgata cagcaggcga atatataaaa agtgctattg
   100561 atagtagcgc aacggataaa gatatggata attatcagca atttgatttg gcggtgtcat
   100621 tacttactca acattggtat ttgaaccgtc aagaagccag cagcgaacgg ataccagtaa
   100681 cggtacaagc attagtacaa caaatgagag gtgcttatta tgccaatcat taagaatgta
   100741 aatgaattga ctgagagagt caagtttaaa aagacaaaac gggtaaaaga tgaagatggg
   100801 cagatggtgg atagtgaaga aactgtattt gaatgttggg ctaatgtgcg ttcacaaatg
   100861 ctaaaagacg tgcttgctag tgtaggtacc attcttgaag gaacgttgac gtttattatt
   100921 cgatacgatc aagattatga actaacaaac gatatgaaag tagcttggaa aaacaagagt
   100981 tataaaatta tttcaatcaa tgaaggaacg gcgtttaaag attatacaac gatcatagct
   101041 aaacagattt cttagaatga ttacacttgt aaacgttgtg ggtatttagt ataattaagg
   101101 tagtaaatga agggttagct gcctgaattt tgcaagactt agctagtcga aatctattgt
   101161 tggactgaaa attgtaggtg tggttgcaaa catgctggac taagaaagat gatagatgat
   101221 gaacgttcaa ttttaataca ggcatgctag gactgattgg atggttttgg caagctgatt
   101281 gtgtagaaat caaagaacac gtctgttgtg gacgtgtttt ttttgttata attcaaaaga
   101341 ggtgatttaa tggacaaagc agagtttaca aatttttatt ggaagtatta tctacattta
   101401 gaaaatgagt ttattaatac cacagatttt gtgatgctcg atgagaataa tttcaaaaca
   101461 ttctctattg aatatcaaaa actactacta gcgattggtt cggagtgtga gattattttc
   101521 aaagaacttt gcggatttga tagcagtagc aataaaagca taactgattt taaagctgtt
   101581 attaaatcta gcgaattaat gtgtttggat aatggtgtta gggttatgaa ttctttaaca
   101641 ttgacttcac taaaaccttt tggagatggg tggcctgaag atacaccaga ttggtggaaa
   101701 atatataaca gagtaaaaca tggtcgttct tcaaattata aggaagcaaa tttggaaaat
   101761 gtgttgtatg ctttagcaag tctatattta ttagaggaat atttgcataa gaaagtaata
   101821 ctagaaggtg aagtagactt tataagtcca gagtcaaatc tatttacgtt atcttgggaa
   101881 agaaaacatt cgtcgatgca aaaagtgacg tttgaaaaag tggattcaaa ttatgtagct
   101941 ccactcattg attttaaaga acaagaagaa atttaaaaac ccccacggct tattgaagtt
   102001 tttgtatgcc atattgtatg ccatttggtt taaatcgatt gaaaaatagt gatagataac
   102061 tctaattcat ttagtcataa acgctgtttt gataaccttt atagttcttt gatatgggtt
   102121 tctcattcat ggcggaagaa gaagaataga acaaaagaat gaattaataa gatataaaaa
   102181 aagggattgt gacaaacgtc ttgtcacaat ccctttttcc aaataaacga tgttcaatca
   102241 gctgcccctc ggcattcgct cgaaaaacgg cgaaatatga agaactattt tgccatttgt
   102301 ttctctaata atcaggtcaa aacgttgact tcacagtctc ttccttaaac ggtgttctcc
   102361 tgccaactcc tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg ttttttgaaa
   102421 ttgttccttg tgactcggag ctgaacggca ggctcacgac ctctttctta aacggtgttc
   102481 aaaagcttgc atctgcggta ataagttcaa ccaaacgaaa attatgagga gctatttttg
   102541 tttggctgtt cttattactt gatgcaggac agctttttca caacctcttt tcgtttagca
   102601 ctaaatttcc ttaaaaagta gaaaagcttt cgaagcctcg tttgtttctt ttactgtata
   102661 ataaaagaaa agagtaacaa tcgctaaaag atataaacaa gagaaaggaa atacaagatg
   102721 attggtctga tttattttgt ggtgatcgtt ctggcgaaca cagttggcgc tatttccggg
   102781 atgggtggag gcgtgttgat caaaccgatt cttgatctta tcggcgccca ttctgttgca
   102841 gggatttctt tttactcaac agttgcggta ttcacgatgt ccattgtttc gactgtgcga
   102901 caagtatcaa gcgggaaatc actgaattgg cagattgtcg gttgggtatc tagtggagct
   102961 gttgttggag gagtagtcgg caacattgta ttcgaagttt ttttacagtt atttgaaaac
   103021 gagaagcatg tgcagctgat ccaaattttt ttgacggtat tgaccttggt ttttgcgttc
   103081 ttctacacca agcatcatca gccaaaattt catttgacgt catggacttg gtaccttata
   103141 tgtggggggg ttctgggctt tttagctagt tttttaggta ttggtggtgg accagtcaat
   103201 gtttctttgt tgatgctgat gtttgcattg ccaataaaag aagcaacact atattctcta
   103261 tctacgattt tcttttctca actggcaaaa ttggtaacga ttgctttaac aagtagcttt
   103321 atgcgctttg atttatctat gttgttttat gttatccccg ctgctgttat tggaggtttg
   103381 tggggagcaa gatttagcag ggttttgtct ccaaaaaaag tgacctttat ttttcaagta
   103441 attgtcctcg tggttctttt ggtcaattgt tacaatgcct ttgtcatctt acaaacatta
   103501 ggagaatcta gactaacgca agtcaaaatc actgaaacat attgttctaa atgaaaaaaa
   103561 gagtatacta tgataaaaaa attcatttta aaaaatcaga aattggctgg tgatttggat
   103621 gttacgggaa atgcgtttag aagatgcaga agcactacgt gaaatcaatg cggatcaatt
   103681 agggtatgat ataccattaa ccgttacggt gagacaaatg aagaaactgc tgaaggagcc
   103741 agagaaaaat tttttcttgg tttatgaaga tcctacttct aaaaaagcag ctggctatgt
   103801 acaagcggag tcctatgaat cgatttttag cgatccaatg ttcaatatta tggcacttgc
   103861 tgtgtctaaa tatgctgaaa aaagaggcat cggaaaggct ttgatgactg gcgtagaaga
   103921 agaagcgaaa aaacgaggaa taacgacaat aaggcttaat tctgcagaat acagagtaga
   103981 agcacaccaa ttttatgaac acatcggtta tcattccgat aaaatgcaaa aaagattttt
   104041 gaaaatcctg taaatcttta tcaaaaaaat acccgatcac caagccgata cttgatgatc
   104101 aggtattttt gtttttttga ctaagaaagt aattgcatca agaaaccaga gatgatcaaa
   104161 acgatggcac caccaagtcg attgcccatt tgagcaaaag caataagttc catccgatta
   104221 gctgcagata agacggctac atttcctgtt ccgcccatcg agttgttgca taagccagca
   104281 gtaatagttg attcaattgg atataaacca aacaaacgac cgacaaatcc tgaaacaact
   104341 gaaatcgcga taacacttgt cagacataag acaacaaatt gccatgtcaa agcagaagct
   104401 aatacattca gattcagcaa cgcaataccg ataccggcaa gaacagctgc tgtcaagttt
   104461 ttgacaatca agttattgaa catgatagct gcatcttcat aatactctgg taataggttg
   104521 gcgattttac agatgacaac gataataatc ataaatgcgt aagcatggac tttaggaaca
   104581 aaatagttac caatttgtcc taagatgaag aaagtcaatg ataccatcaa accaacacct
   104641 aactgtggaa tggaaggttt cacagttcga ggttttccat cgccaagatc gcctttttcc
   104701 attaatttac cattgccgtt gaattttggc attgattgtc cagcacgagc aactaatgct
   104761 gcaccgatga tagctaagac atttgtcatt gctgtcgcag gaatcaggcg ggaaatgata
   104821 gaagaagcat ccgtacctaa aacatttgca taaatcgttg ataaaggaac ggctccggct
   104881 ccaacaccac cagccatcgc aggtaatgag atgtataaaa cggaatttgc aaaaccattt
   104941 ccgataagca tgcccacgag tcctacggca aaaaaggaga cgaccattga aataagtgca
   105001 acgggaataa aacgaacaga agctttcatc aatagattac ggttcatacc aaggatgcta
   105061 ccagtaatca aggcagcaat ataaaagtcc caaaagccca tattgctgac aaagttttct
   105121 gtggatttca ctacatattc aggtaagatc ccaaatgtag caatcgcagc agaagcgaag
   105181 atcgcaaaaa cggaaccacc acccaaataa cttttgacga tagggatttt tgtcccgata
   105241 aaatgaaata aattaccgaa aataaccaaa actgcgattg gtccaatcat attcgtcggt
   105301 aatttcccta aagccattgc aatgatcagg acaaggacca tgatcaaata aagtggcaaa
   105361 cttacgccgg tgatttttgt ggaccaaaaa ccttcttttt tcttttcctc aactgcagtt
   105421 gcagttgttg ttttttgtgc catttttaca gatcctttct tattctgtat catatgaaaa
   105481 aagccaaaga aaagcgacaa aagagcagga gttggctggc cgctgttttg ccgcagtcgc
   105541 ttttttcttt ttagacgtca tacaaagttt ctgtatgttc ttttactata acgatcttaa
   105601 tcttctaaag atttatattc aggaacccat ttagcagatt caactgcttc tttgatatcc
   105661 gtgatttctt cacgattcaa gttttgtgcg acaactgatt ttgcgacagt ttcagcaatc
   105721 gtttgagaaa attcagtgat ttttgctact ggaggcaaga tagctgctcc tggttttgtt
   105781 acatcaacga ttccgcctaa tgctcgacta gcttgagaaa tgatttcagc atttactcgt
   105841 gtagctgttg aagcaatcaa accaagtcct aaacctgggt acatcaaagc attatttgct
   105901 tgaccgattt ggtaagttac accattatat tcaacatcag cagcagggat acctgttcca
   105961 actaatgctt ttccgtccgt ccattctatt aaatcttttg cttttgcttc tgccaatttt
   106021 gttggattag acaaaggcat gatgatcgga cgtggtgtat gagcagccat ttctttcacg
   106081 atttcttcag tgaatgctcc aggttgggta gaagtaccaa tcatgattgt cggatggatc
   106141 gcttttacaa ctgcttctaa gtttgtcaat tcatcagcat tagtgaaatc tgagcgtttg
   106201 cgagcgaatg ggatttgacc aggagtcaat ccttctgtat cttcaaacaa taaaccttgt
   106261 ttatctactt gatagaaatg ttggcgtgct tcttcttcag gtacaccttg acggatcatc
   106321 tcatctaaaa ggatgtttgc gatacccaca ccagcagtac cagcaccaaa tgtcaaaatt
   106381 atttgatctt tcaattgttc accggatata ttcaatccgc ctaatacacc tgctaggaca
   106441 acaattcctg taccttggat atcgtcgttg aaagtagtga tttgatcttc gtatttttct
   106501 aagatcgctg cagcatttcc tcgtccaaaa tcttcccaat gcaataacaa ttctgggaat
   106561 aattcggtag ctgattgaac gaattgatcg atgaattcat agtaagtttc gccttctaca
   106621 cgagcatgac gattgcctaa gtataaggga ttattcaata attcttcatt attagtacca
   106681 gcgtcgatcg atacaggcaa aacttgtgct ggattaatac cagcagcagc agtatagacc
   106741 atcaatttgc cgatagcgat gtcaacaccg ttcacacccc agtcgcccat acctaagatt
   106801 ccttctgcat cagtcacaac aatcaaacga atgtctcgtc catctgccgc gttcttcaaa
   106861 cttgctttga tgttttctgg ttcgtcgatt gaaagaaatg cagcatcttg cggtttcaag
   106921 aaaatttcgt tgtattgttc gattgaatcc gcaacgacag gatcataaac gacaggcatg
   106981 aattcaacta aatgttcgcc catcaattta taaaacaatg tccgattcgt attgaataga
   107041 ttcattaaga agatacgttt ttccaaatct gtttgtttgc ttaagtattg accataagct
   107101 tgaacagatt gttgttccag tgtttggaca gtacttggaa gcataccagt gattccatat
   107161 tttgcccgtt cttcttttgt aaaagctgtt cctttattta gaaaaggatt atttaataag
   107221 tttaaccctg taagcatgaa tgattcctcc taagttttaa tacaaacgaa gtatagaagt
   107281 cagatgatga tatagtcaaa aagagcaggt atgataaatt ttatttatat gtaatatagc
   107341 caaaagaaat caaaatagca gtatttgttc tttttatatc gacaatcctt gcttgaatac
   107401 tatgcgtatc tcttatatgc tagagaaaga aaagtagaag aataagggat agatggaatg
   107461 gataaatagg atttctgagg aaaaaagcac atgatagcgt tgataggagt cacctatgtc
   107521 aggtgataaa aatacataag tagaaaatta agaggaggaa gagaaaatga aagaagttca
   107581 attgattaga aaaagcgaac tatcagaagg cggttgcaat gcatgtggcg tagtagaagc
   107641 gacaagctat acattaaaat tagattccaa tcaagcaata atctctgaac tgacagtagg
   107701 cggtcttgtg gacagtctgg cgcttgctga aggttttact ggagaagata tttatgagat
   107761 gttttcagaa gtccgtcagt taaaaaaagg agaaaaatgc atagaggttc atcatgagag
   107821 cccgaatgtg aggttcaaac gtggtgataa tgagatgatc ttcaagaatc atgtgtctga
   107881 tcacactgaa ctatatgaaa tcgtgaatca aatactgtct gggatttttg aattaggacc
   107941 ttatgagttc aaggaagaaa acggtaatcc aaaattaaat gaagaatggc aagaaacgat
   108001 tgaaacacaa agaaacaatc cacacttatt tcaatagtct tccacagtat ttcgtaaaaa
   108061 tgttgatgaa aaagaaacac cgtgaatcag aggtcgtgaa aaacctgccc tgcatcaagt
   108121 aataagaaca gccaaacaaa aatagctcct cctatttttc gtttggttgg gcttattacc
   108181 gcagatgcaa gtttttgaac accgtttaag gaagagactg tgaagtcaac gttctgacct
   108241 gagtattaga gagacaaatg gcaaaatagt tcctcatatt ttgccatttt ttgagctaat
   108301 accgagggtc agctgattga acaccgtttt atcgagaggt tgtgaaaaag tcgcttagtt
   108361 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttgactt
   108421 aataccgaga aaatggcttt tgaacaccgt ttattcaaaa aaagggactt tgacactact
   108481 tttgtcaaag tcccatcggt tttttatcta tttttattac aatcatttct tccagagtag
   108541 tgacatctgg aatggctgaa atgtaaacag attatttgcc tgtatcaatg tcgaagagta
   108601 atataaaaag tgtaaacgct atcatattgt tcatcattca aattttatag agagaaggca
   108661 ttgagatgga aaaaacatat caagcaggta caaatgaagg aatagtagat tttatcaata
   108721 tggaagattt agaaatcgcg gcttcacaag tgattccagc tggtggttat ggttacatca
   108781 gcagtggtgc tggcgactta tttacttatc aagaaaatga acgggcattc aatcatcgtt
   108841 tgattattcc tcatgtatta agagatgtag aattacccga tacaactaca cattttgacg
   108901 aagaaatgct gactacacca atcatcatgg caccagttgc tgctcatgga ttagctcatg
   108961 tgaaggcgga gaaagcttct gcaaaaggtg tagcagattt tgggacgatc tatacagcaa
   109021 gttcttacgc ttcttgtact ttagaagaga taagagaagc aggcggggag aaagcaccgc
   109081 aatggtttca attttatatg agcaaagata atggaatcaa tttggatatt cttgaagtgg
   109141 ccaagcgtaa tggagcaaaa gcaatcgtat tgacagcgga tgcaacagtt ggcggaaacc
   109201 gtgaaacaga ccgcagaaac gggtttacct tcccgctgcc aatgccgatc gttcaggctt
   109261 atcaatcagg agtaggccaa acgatggatg ctgtatataa atcttccaaa caaaaactta
   109321 gtccaaaaga tgtagaattt atcgctgctc attctgattt gccagtctac gtgaaaggtg
   109381 ttcaatcaga agaagatgtc tatcgatcat tagaatctgg tgctggtgga atttgggtat
   109441 ctaaccatgg tggccgtcaa ttagatgggg ggcctgctgc gtttgattcg ttgcaatatg
   109501 tggcagaagc agtagataaa cgtgtaccaa tcgtgtttga cagcggtgta cgacgtggac
   109561 aacatgtttt caaagcaatc gcatcaggtg ccgatcttgt tgctattggc agacccgtta
   109621 tttatggatt gtctttagga ggaagtacag gagtccatca agtgtttgat ttctttaaaa
   109681 cagaattgga aatggtcatg caattagctg gaacacaaac agtagaagat atcaaaaaaa
   109741 taaaattgcg tgagaatcgt tttatttaag aaataaatga cgaaaaaaat aggagtgcgg
   109801 cagatgatgc cgtactccta tttttatagt tcaaggatgt cactaataga ataatttgaa
   109861 acgaaaaaat aaaattgaaa tattttttca tacttaataa aaaaggaaga atattcatgt
   109921 aaacgatttt attgtttttt tctcttttat acaaaaaggg tcataatttc aagtaagcgg
   109981 atgaattcat gaggattttt tgcatactgt ctctactata cttaggtcac taagaaaaag
   110041 gagtgattta ttatgaaaaa gaacatgatt gggacattat tactttcaac acttcttcta
   110101 ggcggcttgg caacaccagc ttttgctgag ggtcaagcga cttcaaaagg agatattacc
   110161 tttactgaac caacaaatac cgttgagcct cttaacccta ctgatccttc aaaaccagta
   110221 gagccagcag atccggaaaa tccagcaact ggccagacag gttctttaac attggatgta
   110281 gttcctgaat tgccatttgg tacacacgag attgaaagcg gaacaaaaac gtatcaagta
   110341 gacgcttcaa aaaacgatac accttatctc caagtatcag atcgtcgagg tgtaggtgca
   110401 gatggccaag cacaagggtg gaacgtgaca gtttcggttt cagattttgt aaacggttca
   110461 caagttttgc aaggcgctga attagatttt ggaacatcca ctgttaaaag tacgtcagat
   110521 aacgaatcca ctggaccaac tagtcaaact gtaactgggt tgagtaaagc aagtgcagcg
   110581 acaccaatat ttacagcagc aaaagatcaa ggtctgggaa catggctttc tgtttatgat
   110641 ccagcaaata tcacactgaa agtgccgaaa gcagcagctg gaacattcac ggctgatttg
   110701 acttggaacc tcgtggctgg cccagtagca tagagtatga aataaattca acaaactgcg
   110761 gtgaaaatac tataggtgaa ggatttttta ccgcagttgt tgttgaaagg atgtggtgga
   110821 caatgaatca aagtaaacga ctgtttgttt cgtttttttc tattttaagt ctatttttca
   110881 tttttccgtc aatcagccaa gcaaaggatt cagcaggaga ctttggaatc aaaccggtat
   110941 ttccagagaa ccaaatagat aaagcaatcg gttattttga tttattagtg gcaccggaac
   111001 aaaatcagac actagaagta attatcagca attcttcaga tgaagaacga acatttgagg
   111061 tctctgtcaa tccggcggta acgagtgatg gtggaacgat cgattatagt caaaaaaacc
   111121 caacgttaga tgagacattg ccgtttgatg tccgtgacgt tttgctcatt gcgaaaaagg
   111181 aaatcaatgt atcagcacat gcagaaacaa ctatacctat agaagtgaaa atcccagcaa
   111241 aaagctttaa gggacgtgta ttagcgggta ttcatgtttc acctaaagaa gaagctgaaa
   111301 cagaaaatgc tacagaagga gcacaaatca aaaatcgtat tgcttataat ctcgcagtcg
   111361 ttttacaaga aagtcaagag acgatagaac cggatctgaa acttttatca ggagatttgg
   111421 acgaggtcaa cgcaaagcca actgttcagc ttcgtttcca aaatcctcag cctaggatta
   111481 tcagcaattt gatatttacg agcaagatct tttatgaaaa tcagctttat attgaaaata
   111541 cgtctaatga atttttagtt gcaccaaatt cgaattttca cttgaatctt gatttagctg
   111601 gtgataaagc aaaagccggc gattaccgcg cagaaatcat agcaaaatca ggcgacagta
   111661 atgaatggcg atttacacaa aactttacga tcaaaaaaga aaaagcacaa aaagtaaatg
   111721 aaaattccgt ttttgcagta caagaacaat ctttcccatg gctttatgca ggattggttg
   111781 ctgcagttct agtgttatgg ttgattttgc tgtttttgtg ggtccacaag aaaaagaaaa
   111841 aaggagaaac aacatgatga ggtgatttcg ttgaaaacat cgatcttatt gggagggatt
   111901 attgcaagtt tgctgttctt cagctggcaa gagccagtcc aagcagatac caaaagttca
   111961 ggtacgatta cattttatga tctaagtcca gttccgcatc caagtggttc agtcaatggc
   112021 gcatcaaaac agcagacaca gctaccgatt tataaagaat ctaatcaaca tggcgtattc
   112081 ccggagacga atgaagaaaa gatccgtttt ctttatttga tcattggtat agaattatgg
   112141 cttcttgcgt ggttgttgtt atggagaaga aaggaaaagg aggatgaaca tgttgctaca
   112201 aaataaattt tcattttttc gctccttctt ttttacttcg ctttttgttt taagtatatt
   112261 tatcttttca ccttcggcag aagctgttgg agcaacgcct cctgccattt cttatgatcc
   112321 atacacgtat gaaaatatcg tacctccaaa tggtggacgt agtgcagtca atggcgggaa
   112381 gcttgtccaa ctaactgggg ctaaccaatt gcctaaattg actgctactt atttgaacga
   112441 tggttcaaat gatccttgga aaggtggcac ggtccgtttt cattgggaaa gccatgtgaa
   112501 actaacaaat gtacttgatt tggtgaatat cactgcacaa ggatttgcat ttgtggcgat
   112561 catcaatttg ccggaacatg tatcaagcag tgatgtatta aaagcaattg actggaacaa
   112621 cgcctacttg tatattgccg gtgcccccat caaggttact agcggtagtt tattatcagc
   112681 aggaagccat accttgcgtt ttgggatggg gtcgtgggat acaaatactc tattggcaga
   112741 tattattgct attattacgc gaggcgaatt caacgtggac gatatcccgc ttcaatttga
   112801 tgttacagct aatgtcgcga agatgacaga aaacggagat aaatatgata cgagtccaaa
   112861 caaaattttg accaagggta aattacaacc ttctatgtct aaaaaggccg ttttttctgt
   112921 tgatttttat gattcgaata atattatcca agatcgtatg ttcaattcga ttttagctcc
   112981 agctgcaggt agattttatc ctaaattaac tggcggaaac ttgaatccag gagcatatgc
   113041 attttctgca agtgcagaga taaatacgtg gaatagttac atctcaacaa gtgattcaac
   113101 aggaaaatat aatacatttg ctgataccac agaatacgtg gatggaaata accagaacct
   113161 tccaggctct accaatagtc ggacattgac gatggggctg actgaaggac gttttgatca
   113221 gttgccattt agtcgctttg atcgcgtagt caactatttt acaggaaaaa acgttacaca
   113281 aggagcaata ctgactcata ctccagcaag cacaagtgga ttgaatcaga cagccaatat
   113341 cgtgtacagt gggaaagatg cgcaaggcgg attgctttct cctgttgctt taaaggtatt
   113401 ggagcagtac aagctagacg gaacggtttt accaacaaac ttaacagccg atagtcagtg
   113461 gggaaagctc ctttcttctg catcgtacca agtcacggcc aattggtcag caactgaact
   113521 aaaaaagggt tcgatccatt acaggcttta ttcgaaagga acccaaaaat tagcgacagg
   113581 atggacagat caacttttcc aaacgatcca atcaaataac gggggtgtaa atacagctac
   113641 ttctacacta cctgcattgg cttcagggca atattatttt gattatcgct tggtggacga
   113701 tgtacttact aacatgtatc cgtcttttgc gtacaaatgg cagtctgaac aagctggaat
   113761 caccactctt cctcaaataa cagtggctaa ttttccatct gtgagtgcag atagtcagct
   113821 aaaaaacctt agcagacaac ctgagacagg agatcccctg tctgctctat caggagatac
   113881 gattcaagaa aatgtgacat tcacgttaac aaaatctggg gatgcgataa ccgacaaaaa
   113941 aatcacgatc tcacttcctg aaaatactac ttatatcaat ggttccttaa aattaaatgg
   114001 tacaactatc gcagataccg gcattcaaca aggagtttct gtcccagcag atctattgag
   114061 caaaatagga gataccattc atcttactta caactatcaa ttaaatactg tagatacttc
   114121 tgtacaatcc gtcagcatac tgacaaaggc agctgtactt tccagcaata ttacattagc
   114181 agatggtgct aagcttccta acccagttgt ccaaacatct gctaaaacaa ttcttgtccc
   114241 caaacaagaa ttaacattag ttaatgtacc agatgatttt acatttggta acgatctccc
   114301 caaaccgttg aagacaagtt attatgaagc aaaaggcgat ttttcttttg atgtgcggga
   114361 tactcggctt ccaagcacgt ctccttggca actgacaggg acattgactt ctttatttaa
   114421 gaatgatcaa ggtcaagaat taagcggaac aaaattatac ttcaatcatt ctggatcaaa
   114481 acaattgatt cagcaaggtc aaaacacgtt gatttatgag agtgatggaa cagctaaagg
   114541 agaggttctc gttgactttc ccgataccga tggtttactc ttagaggtaa atagcagtac
   114601 gaatgctcaa cctggcgcaa cgtatcaagg catggtcaca tgggaactga cagctgggcc
   114661 aaccagctaa gtgattagaa tttgtatgga tctgtgataa ctgtcacaga tcctcttttt
   114721 caaggatgag aaggaaactt ctgctgttaa aatcagaaat gcttggagga gtggacttgg
   114781 aacttttcag tttgcttgaa aaaaatgaac aacggcaaat caaacttttc caacttttat
   114841 tagaggaaaa aggagagatt tccgttaata acgtacttac ccgcttatca gttgatcggg
   114901 ctaccttgaa agaagatctg ttttatttac aagctaactt ggaaaatatc aaacaattgg
   114961 aaattgttat ccaagaaggg aatattttat taatcagaca tggaaatata gcagcagtcg
   115021 atgtttatta tcattgctac ttgcgctatt ctacaaaata tcagatactt ctttatctgt
   115081 tggaaaatgg ctcatttgat cggcagaaac ttgtcatgga acttaatatc agttcggcta
   115141 ctttgtctcg cagagtgaag gaattaaatg attctttaaa tgagtttcat ctgaaaataa
   115201 aaagcgggcg gctgaccggt ccagaaagtc aaatttgtta tttttatttc caattatttt
   115261 ggtttggacg tccttatctt gaaaacaaaa aagaatacag tgatttatca ggaaacactt
   115321 ttttagatct gctgcaaaaa gagtttagtt ttactattac agaagaagga cgattgaaat
   115381 ttgaaatatg gctttccatt atgaagaagc gaatgcgctg tacgaaaaaa tccaaaccag
   115441 attatagcaa tcttcctgtt ggattgtttc aaacgacaca atttttattg gacctccata
   115501 catttttgac ccgttacttc tttcaatatg cctttccttg gaatcaaaat gaaacgttgg
   115561 ttttttatct tttcatgatc agtaacttta cattagatac acataactcg catgtccagg
   115621 agtttttgat aagcgaaaaa tacaaagacg acgtagtagc tcaattgaat cgaacgttta
   115681 aacaatcgat agaagaatat ttctcttttt atgtttttcc agaagagatt tcgcaaaaga
   115741 ttgcgttgac agtgatgcaa aatcacgctc attttgccta ttttagagga tggatgaata
   115801 tttttggcca agaaggatta aaacagcggt taactgaagt agaagataaa caattcctgg
   115861 ttttatgtga aagattggtc agacaaacat ttgctattgt tgattttact gaagtagacg
   115921 aggaaaatgc ccgctttgaa ttatttgctc gctatgcatc tattttgcgg acgctgatca
   115981 agcagttaga attacgtctg caaattgctt atgatcttcc ttatgaaagg ctaatggcgg
   116041 atatcgtgaa agaaatcatc caggaacaat tgctgaatac gatcaaatat gatttagtcg
   116101 aatacgagaa agaaaagcag tatgatatca ttttgactag ccaattgaaa gagtacccgt
   116161 cacagaagtc cgctaaagtt tttgtattca ctagcaacga atataattat gatttcccct
   116221 atttgaacca atttttaaaa gaatgttatt tagaaaaatt gaatttaagg atgaataaaa
   116281 catgaaaatc gtgatcattg gtgcttccca tgcaggcatc acagctgccc taaatctaag
   116341 aaaacttcaa ccgaaagcag aagttttact gatcgatgaa gatcataaag atggactagg
   116401 ctatgtttca aatggaatca acctttattt aaaaggaaag atacgtagtt tagctgaagt
   116461 tgcccataac atgcggactc ttcaagaaag tggggcaaaa ctgatcaccg aatggagagt
   116521 aactgaactt gatccagaca aacatcagct catcctcgca tccaaagaag gaaaaaacga
   116581 gaccataacc tatgataaat taatcgtagc aacaggttcc tctcctgtca ccttgtataa
   116641 acaaattgaa gctgaaaatg tctacaccta taaaaatcta gtgcaatcta agcaagtact
   116701 tgccgcttta aaagaagcaa aagaagtagt gatcttcggt gctggttata ttggattgga
   116761 attagcagat gctttgagaa ataaaggata cattattcat ctagttgatt atatgccgaa
   116821 tgttttaaca agatatttcg ataaggatat gatcaacagt tttcaaaatc agctgcaaac
   116881 taagcaaatc aatttttatc caaatgaatt cttgatcgat tggaaaaaat cagaagagaa
   116941 agtagtatcc gtacaacttc tatctcaagc aataaaggca gatatggtca ttttttctgc
   117001 ccaaactcgt cctaatacga cattgttaaa agaaaaagtg gctttgtatg aggatgagac
   117061 tgtgatagtc aatgaatatc tgcagacaag tgatccggac atctatgcga ttggagatat
   117121 cgtcccagtc agttttgaca aaaataaacg gcatctcttt ttacctttag ttactcgagc
   117181 tgtacatatg gcaagagcag tagcgctaac tttgagtggt cagccaaccg cttacgacct
   117241 cagacaaaag ataacagcag cagtcattat agactacttt ttaggaactg tagggctgac
   117301 agaagatgaa gcgccattct tggaacaaaa taccggttcg tgttcaggag aatttgatct
   117361 atttccacaa tacgatgaag aaaacaaaac agtaaatgca aagctgatct accatccgga
   117421 tacattagaa ataattggcg gacagctgat aagccaagaa ttcttattat cagatttgaa
   117481 cctgcttgcg gatatcgtga aacacaaaac tacgattcca cagctagcag ttgaggattt
   117541 tggatttctg tctgaacaca cctctcgttt tcactacctc aatgagttag cttttaaagt
   117601 actagctaag agagcaaata gcaatagagt agataaaaga ctgtgagaaa tatcacggtc
   117661 ttttattgtg tggggatata ataaatatta atcaatcgag aataaaaaat tcatataatg
   117721 aaatagcgga agaatttttt gagcataata ttcgccaaaa atttatcaag tgataaaatt
   117781 gttgtataaa atgattattt caggaggagg agttaaatgt ttttagcatt aaatgaaatt
   117841 atgcattcaa agttacgtta tgcgttagtt gcaggggtga tgtttttgat cgcctacctg
   117901 gtattttttt tgactgggtt agcttatggc ttagctcaag ataatcgaac agcagtcgac
   117961 aagtgggagg cagattcgat tgtgctttcg aaagatgcaa attcaaatct agggatgtcc
   118021 atgattacta agaagatagc agaagaagtc gaaggtggga aagtggcgta tcttgcccag
   118081 acacctggag tcgtgactag taaagacagt acagaagaag gaaaaatcaa tgttagcttc
   118141 ttcggtatcg ataaaaatca attcatcatg cccaatcttg ttgaaggaaa agcttttgat
   118201 aacgatgatg aggcagtagg cgatattagt ttgaaagaag aatatggtct agccgtagga
   118261 gatacagtta agttatctgg tagtgacaag acgtttaaac taactggctt tacggatcat
   118321 gccaaattca atgtctcacc agttctctat acaaccatca atgcttacca agagatccgc
   118381 ttcgaaaaag aagatacaag tgaaaatgca cggattaacg caatagttgt cagaggtaaa
   118441 atcagtaatt tacctgaaga tttagaacag atcaaaattt ctaaattcat caatgaatta
   118501 cctggatata atgcgcaagt attgactttt ggatttatga tcggattttt gatcgttatt
   118561 gcggctattg tgatcgggat tttcatttat gtactgacga tgcaaaagat taatatcttt
   118621 ggtgtaatga aagcacaagg gatcacagga ggatttattg cgcgttctgt agtggcacaa
   118681 acattcattt tatcatttgt tggtattctt cttggtctat taggaacagt gggaacatcc
   118741 cttgttctac ctgatgcggt tccattccaa agcaattggc tgttttttgg cgtcattagc
   118801 ttgttgatgc tggtagtagc tgtgttaggc gcactatttt ctgttcggac aatcgtaaaa
   118861 atcgatccgt tgaaggcaat tggataagaa ggaggaagag catgaaagca atagaattta
   118921 aagaagtaga aaaaagtttc caagatggcg accaagtagt taaagcattg aaagaaacaa
   118981 ccatgagtat cgataaaggg gaatttgttg caatcatcgg tccttctggt tcaggtaaaa
   119041 gtactttttt gactttggca ggcggattgc agacgcctag cagtggctct gtatttatca
   119101 atgatcaaga attcagtaat caaaaagaaa gtgaacgcgt taaactacgt tttaaagaaa
   119161 ttggctttat tttgcaagct tccaatctgg ttccttttct gacagtaaaa aagcagttat
   119221 tattagtaga taagatcaaa catgaacatc gtcaaaaaga agcaaaagaa ttatttgagc
   119281 agcttggcgt ggataagttg atcaacaagt atccagaaga actatctggt ggagaacggc
   119341 aaagggtcgc aatcgctcgt gcattgtaca atgatccgtc cattattcta gcagatgaac
   119401 caactgctag tttggattca gaaaaggcac gagaagtagt cgatatcttg gcaaaagaat
   119461 caaaagacaa aaataaagca acgatcatgg ttactcacga tacacggctg attgacaaat
   119521 gcgatagaat atttatgatg gaagatggtg tattgaaaga aaaagaataa ccaaaaagca
   119581 ggtggaaaaa tggtccatcg atacaacaaa gatacacagc aaaaaatttt gaaagaagcg
   119641 actgacttgt ttatgtccaa gggatatctc ggcacttcaa caagagagat cgcacaaaaa
   119701 gttgggatca cccaacccaa tctctatcat tatttcggtg ataaagaaaa actatatacg
   119761 gcagttttag aaaaccactt aaaagatgtt ggaaaagctt tgagagaaat cgttcaaaca
   119821 agcgaaacgg gatttcaagt aaccttgaca aaaatggcgc agtttttgat tgagacacat
   119881 ctggtcgatt tgtttctgat gcttcacgat cttgaaagca atctttcaaa ggaaacaaga
   119941 gatcatcttt tctttctatg gaagaaaaat tatcgtgaac cttttgaaga aatcttttca
   120001 gcaaaccaat ctgtgctaag aaatgggatt tcaaaagaaa tcgcagcaag gcattttttc
   120061 ttagtgttag caccttacat cactcggtcg aaaagacctg tggaagaacc gctaaatgtg
   120121 gcgcaattga tcgatttata tttgaatggt gtattaggag aagcgtaaag ctgttatata
   120181 gggagaaaaa tagttaggtg aagaagcaag atatgacgaa gatcgttata tcttgttttt
   120241 tttatatagg tagtttttca taaatacaaa tttgttattc tctcatgatt atcagggtat
   120301 atttttattt attatgtcgg tgttccagga agtgttttaa tttaataacg aagaaattaa
   120361 ttgtttgtag aatgaaggaa actatttatt gggtagatga atcaccacga atagtcaaaa
   120421 ggagatttca ggatatgcca aaaatgaaaa aaatcatttt agcagtaatg ttattttcta
   120481 cggttatatc ttttacagcc ccttttttgc gtacttaagc tgattttaag ttttgaccct
   120541 tagtctggat ctacgaggtg agagtcaaga atgaaaaaaa taattggagc ttttgtattt
   120601 gcaatcatta tcattattat ctgttttcaa atagatgttt ttggtcaaaa aaagatagtt
   120661 ggacaaacgg tcagcagtac gaacagtacg gttacaaaga caaaggaatc atttgatgat
   120721 acagaacaag agacattaat caaaatcgga aaaaatgacc gtacactata tacagatgac
   120781 tcgctaacta ccccacttgc aactataaac ggcggagagc tggcggagtt tctttctgaa
   120841 acaaaaaatg cttatcagat caagacaaat gatggctata ctggatattt agccctcgaa
   120901 gatggaacca aagtcacaaa gaatatacaa acaaaaccgg aagagctatc tgatgcagtg
   120961 attgtattag atcctgggca tggcggaaat gatactgggg cattaagcaa tgatgaacaa
   121021 acagaagaaa aagatataac attaagtaca gccataaaag taaaaaaggc gctagaggat
   121081 gctggagcaa cggtttattt gacccatact acagatgaat tagtacagct tggagatata
   121141 tgcgactaca gtgaagaaaa aaaggcagat gtctttatca gtttacacgc agactctacg
   121201 gaatatgcga atgaagcaac tggaatcacg acatattatt actatggaca agaggaaaca
   121261 ttagcacaga caattgctga ctccttcact gatctgcctc tggattcaag aggaatctcc
   121321 actgggaact atcaagttct tcgagaaaat cttcaacctt ctattttaat cgagatgggt
   121381 tatatgaata atgatagtga tttaaaggaa ttagtaacag acagttatca gcagcagatt
   121441 gctgcaagtt tgacacaagc attgaccact tattttcagt aataaataag cccagccaaa
   121501 caaaaatagc tcctcatatt tttcgtttgg ccgggcttat tatcgcagat gcaagctttt
   121561 gaacaccgtt taaggaagag gttgtgagcc tgccgtttaa ctccgcgtca caaggagcaa
   121621 tttcaaaaaa cagcttctca tgtttgatga ctattgtgac ttgtgaccga ggagttggca
   121681 tgagaacacc gtttattcgg aacaagggac tgtgacatga cttttgtcac agttccatta
   121741 tttttttagc agcttcaaag ctttttctgt attggcaaaa tgaagtttgg catatttttc
   121801 aagcataggc atgccgcctt tctccaatag ttttgccgct acttggtcag atttcgttcc
   121861 agctcctgat ggaagggtga ttccagcttc agctgatgct ttgtgtaact cttccaaaaa
   121921 gctggcacga ctgatgatag aagcagcagc aacagctaaa tgatattgtt ctcctttagt
   121981 aacgaagtac agtttttctt gtaattgatt tttttctaaa cgcgcatatt ttttaaaatt
   122041 tgcttccggc gtaaactgat cgatcaaaat cgcttctggt ttttctggct cgattttttt
   122101 tagcagcaaa tggatcgctt gattgtgcaa agcgactttc atccgaacgg cattgtattt
   122161 tggttggatc tcattgtatt tttctggaga taagacaagc agtttatagg ggatggtttt
   122221 ttttaagatg tcagcaagct ggatgatttg gctatctttt aactcttttg aatctctaac
   122281 accaagttta cgtaattctg caagctgatc acgtttcaca taagcagcac atactgttag
   122341 cggaccaaaa tagctgccat ttccgacctc atcactacct aaaacggata attgactgat
   122401 attttttggc aaagaagagg ctgtttttgc tggcttggct tgtttatcct gtgcctcccc
   122461 ccatttatca gcttctattg ctgcattcgt accttgaaac atcacttttc cggatgtata
   122521 agcggtaatg gtcgtcccgt ctttttttgc agcaaagcta gtataaggaa tctgtttatt
   122581 taacaaataa gagcgatagt atgctttcat ttgactaagt tggtcgtttg ttgctttgat
   122641 aatgacgttg ctcataaaat tctcctcgtt tttatacata aaactatacc gcaactcacg
   122701 acttttcgtt ttctaagaga ttgcgagaca tagcattttt ttaaagttta tcatataata
   122761 gttaagtatg tgaaaaatat ttgtggagaa cggtgaaaga accaacttca tggtacacta
   122821 aataaaactt tcatcaaata ggaggaaatg attcgtatgg cgcacgaaaa aacacgctac
   122881 aaagcagtga ttgccaacca aacatatact attatcggac gggaaaccaa gcatcatatg
   122941 gatatcgtaa cgaagctgat caatgaacag ttagcagaat tgaaacaatt atcaccacaa
   123001 atggataatg aacaagcagc aatcttgatg gctgtcaatg cgctttcgga tcaattgaag
   123061 aaacaagaaa gaatcttgga gctggaagaa gaaacagcag aattaaagaa aaaaatgatc
   123121 aaatttacag agcttgaaaa tcgtgtgaaa cgcattgaag caatcgagaa cgaagctcgg
   123181 gaagtattga aagaaaatgg tcaagcagat cataccatta aaaatcatgt ggaagcacaa
   123241 cagattttaa acgagaaacg aaaaggacaa attaaacaaa aatcttctaa ttaagatcct
   123301 tttatcaaaa taagaaaaat ttaaaaaagt tagaagagaa aggaacgtag catgctgagt
   123361 ttgctgattt tatttttatt gctaattgcg tttttttctg gagcacgtcg cgggtttgct
   123421 ttgcaaatca tattcgcgat tggatatgta ttgtcattta ttgctgccca gcatttttac
   123481 aaaccattag caagtcattt ggaattatac attccatacc cagctgtcac cccaacatca
   123541 aagttagcat tttttgatca ggtatttgct tttcacctag atgaagcttt ttatgcagga
   123601 actgcttttc tgattatttt gctaattggc tggctgctta cccgatttgt gggggttttt
   123661 gtccatggat tgacgtatgt gccgatcttg agacaggtag actggattgc aggaggcatc
   123721 ctgtcactaa tcatggctta cgttactata tttttgattt tgcagttact tgcctttgtt
   123781 ccattagatg tggtccaaaa tcaattcggc gagcatagtt tcgctcgttt tatcgttgag
   123841 agaacgccat ttcttaccaa caagatgtat gagctttggg taacgaatat tttgaattaa
   123901 ggaaaatagt gctggaaaat atttcgtttt ttgtacagag tagtttattt aagaaggctg
   123961 cgtagcgtat gtccggcaca gctctttttt catagtgaaa gataaggtga taaaatgaat
   124021 aaccgaatcc ttgaaacatt ggaatttgaa aaagtcaaac agatggtcag acaatttgta
   124081 gtgactgcac aaggaaaaga agaactggca gaattggtac cagtgagtga gaaacaaacg
   124141 atcacaaact ggcttcaaga aactgaggat ggattgaaag tgcaacgtct gcgaggcggg
   124201 atccctatac ctaaacttga aaatatccgt ccccatatga aacggatcga gatcggagca
   124261 gacttgaatg gagtggagct tgcacaagtt tccagagtat tgtctacgac aagcgaattg
   124321 aatcgtttca ttgatgatct ttctgacagt gaaatcgaat ttgcacgttt gtatatgtgg
   124381 gcagatcagt tagtcacgat ccctgttttg agccgccgtt taaaggaagc aatcgatgaa
   124441 gacggacgcg tgactgatga cgcttctcct gagttaaaga gcataagaca gaacattcgg
   124501 cgaagtgaac aagctgttcg tgaacagtta gatgggatcg tccgtgggaa aaatgcaaag
   124561 tatttaagtg atgcgatcat caccatgcga aatgaccgct acgttatccc agtcaaacaa
   124621 gaataccgcg gtgtatttgg cggtgtagtc cacgatcaaa gtgcttcagg acagacttta
   124681 tttattgaac caaaacaagt ggtggatttg aataatcgtt tgcgtcaata ccaaatcgct
   124741 gaacgaaatg agattcaacg tattttaagt gaactttctg ctgaacttgt tccccatcgt
   124801 caagaaatca tccacaatgc ttatgtgatt ggtaaaatgg atctgatgaa tgcaaaagca
   124861 cgatttggta aagaagtaaa agccatcgtg ccgggaatca gtgaagacaa tcatgtcgtc
   124921 ttgaaacaag caagacatcc actgatcgat caggaaaaag tagtttctaa tgacatcaca
   124981 atcggaaaag actatcaagc catcgtcatt acaggaccta acacaggtgg gaaaacgatc
   125041 acgttgaaga ctttaggtct gttacaactg atggggcaag caggattacc gattcctgca
   125101 ggtgaagaaa gtcaaatcgg tatttttgag gaagtatttg cggatatagg agacgaacag
   125161 tcgattgagc aaagtctatc gaccttttct tctcatatga cgaacactgt agatatttta
   125221 tcaaaagtca atgaaaaaag tctggtatta tttgacgagc tcggtgcagg aacagatccg
   125281 caagaaggtg ctgctctagc aatcgcaatc ttagatgatc ttggtaaaaa gtctgcttat
   125341 gtgatggcaa caacgcatta tcctgaattg aaagtttatg gatacaatcg ggcaaatacg
   125401 attaatgcca gcatggagtt cgatgtggat acactaagtc caacgtatcg tttgctgatt
   125461 ggcgttcctg gacgaagcaa tgcgtttgaa atctctagcc gattaggtct tgatacagaa
   125521 gttattgatg aagcgaagca gttgatgaat gatgagagcc aagatttaaa cgaaatgatc
   125581 acagatttag agaatcgccg gaaaatggca gagaccgaat atctggaaat gcgtcatttt
   125641 gtttctgagg cacaagaact tcacgatgat ttgaaagaag cttacagcta tttctttgaa
   125701 gaacgtgaaa aagaaatgga aaaagcaaag aaaaaagcaa acgaagtcgt gtcagaggcg
   125761 gaagaaaaag cagaaaaaat cattgctgat attcgtaaaa tgcaacagca gattgggcaa
   125821 ggaaatgtga aagagcatca gttgatcgat gcaaagaccc aattagcaaa tctacatcaa
   125881 gaagaaactt tgaagaaaaa taaagtattg aagaaggcga aagaacaaaa aacgttgaaa
   125941 ccgggagacg aagtgttggt tacgacttac ggccaaagag ggacgctttt acgtaaaaac
   126001 gggaatcagt ggcaagtaga aatcggtata ttaaaaatga atgtgtccga agacgagctg
   126061 acgcctgttg ctcctcaaaa agagccgaca caacgagtta tccatgctgt acgatctgaa
   126121 tctagtagtc acgtgccgaa tcaattggac cttcgaggaa aacgatacga agaagcgcta
   126181 agtgaggtag atcaatattt ggattctgcc attctagctg gttatcctca agtgactatt
   126241 gtccacggaa aaggaacagg cgcacttcgc aagggaatta ctgattatct taaaaaccat
   126301 cggagcgtca aaagctttga gtttgcaccg gcaaaccaag gcggaaatgg tgcgacaatt
   126361 gttaaattta agtaaagctt actaaaggta agcagatggt tcgctatcca agtatagaaa
   126421 tgctatacta atcatagatt ttaaaaatgg aggtctttga tatgtcacaa gtaattacag
   126481 ataagacatt taatgaagaa acagataaag gacttgtatt gattgatttt tgggcaactt
   126541 ggtgtggtcc atgtcgtatg caagcaccga ttttggatca gttggaacaa gaatatgatg
   126601 aagaagaatt cagaattgct aaaatggatg tggatgaaaa tccagaaact cctcaacaat
   126661 tcggtatcat gagcattcca acattaatgt tgaaaaaaga tggtcaagta gtagaaaaag
   126721 ctgttggtgt tcattcaaaa gaacaactac gtcaaatgat cgatcaatat ttataaaaac
   126781 ttacagcaaa aataagctga gggtgggact gaatccagtt ccgtcctcgg tttttttgcg
   126841 gttgagttaa ggttattcaa attaatattt tttgagtata attacgttgt caggctggat
   126901 tgtttttttg acagacatgc tataataact ttaattgaat atgaagggga gtaccagatg
   126961 aacgaacgaa tcaaaaacaa attagcttta cttcctgatc agcccggctg ttacttgatg
   127021 aaagataaaa atggaacgat catttatgtc ggcaaagcca aaatattaaa aaatcgtgtc
   127081 cgctcatatt ttactggcag ccataatacg aaaacggaac ggcttgttag tgaaatcgaa
   127141 gactttgaat acatcgttac agaatcgaat atcgaagcat tgctcttaga gatcaatctg
   127201 ataaagaaaa atgatcctaa atacaacatt atgttaaagg atgataaaac ctatcctttc
   127261 cttaagataa cgaatgaaaa atatccccgt ttggtgatta ctcgtaaagt actaaaagac
   127321 aaagcactgt attttggacc atatcccgat gtcggtgcgg cgaatgagac aaagaagatc
   127381 ctcgatcgat tatttcctct gagaaaatgc aaacctagtc agacgaagga gccttgtctt
   127441 tactatcatt tgggacaatg tctgtgtcct tattatttcg acgtagatcc gaaagtctat
   127501 acgaaaatcg ttgatgaagt gaaaaagttt ttaaacggag gacacgaaaa aatcgagtca
   127561 gagatccatc agaaaatgga aaaagctgct gaaaacatgg agtttgaaaa agctgcagaa
   127621 taccgagacc agatccgtgc cattgagaca atcatgactc gccaaaaaat gacaaatact
   127681 gatttactgg atcgagatgt atttggttat gcgattgaca aaggatggat gtgtgtccaa
   127741 gtgtttttcg taagacaagg aaagctaatt gaacgagatg tttcaatgtt tcctttctat
   127801 aacgaagcag aagaagattt cttgacttac ctcggtcagt tttatcagga aaatgagcat
   127861 ttcattccta aagaagtctt gattcctgat acgatcgaca agcccagtgt agaagctttg
   127921 ttagctacga aagtgctgca accaaaacga ggagaaaaga aaaaattagt tgaattggcg
   127981 aataagaatg cctcagttgc tttgaatgaa cggtttgatc tgattgccag aaaacaggaa
   128041 cggacgatcg gagcagttga acgtttagga aacgcgatga atatcccgac cccaatacgt
   128101 atcgagtctt ttgataactc taatatcatg gggacgaatc ctgtggctgc gatggtcgta
   128161 ttcattgatg gaaaaccggc aagaaaagaa tatcggaaat ataaaataaa aacagtcaac
   128221 ggaccggatg attatgcatc aatgagagaa gtcatctatc gcagatattc acgagtgatt
   128281 cgcgaagggt tgcctttacc tgatttgatc ttgatcgatg gaggaaaagg acaagtagat
   128341 gtggcaaaag atgtcttaga aaatcaatta ggaatcgata ttcctattgc tggcatggca
   128401 aaaaacgata aacataaaac aaatgagttg ctattcggta tagatttggc tgtagtaccg
   128461 cttgagcgaa attcaccaga gtttttctta ctccagcgaa tccaagatga agtccatcga
   128521 ttcgcaatca ctttccatcg gcagctacgc agtaaaaaca gttttgcttc ccgattagat
   128581 gaaattgaag ggttaggacc aaaacgaaaa aaaatgctgc ttaaagagtt caaatcgttg
   128641 aaaaatatca cagcagccac gacagaagaa ttacaagaaa tcggactgcc gaaaaatgtc
   128701 gcacaaaacg tatttgataa attacatcag gaatgaggaa agggcacttt acaaagaaag
   128761 taaagtgtcc tatcatttta tgtacaacaa aaagagcgtt tacgttttgt tggtaaataa
   128821 taaatcgctt acgtctggtg tctaaggata tatgtgattt cttgaagaag gagtgaagaa
   128881 atgttagaag tcaaggatct tgttaaaact tttgggaact acacagctgt cgatcatgtc
   128941 tctttccaaa tccccgatgg aaaaatcatg gggctaattg gccaaaacgg ggctgggaag
   129001 acaacgactt ttcgtttgat cttggatttt ttgacacaag accaaggaga agtgttatgg
   129061 aatggccatc atcttgggga gaaagattat gatgtcatcg gttatcttcc ggaggaacgg
   129121 ggactttatc cgaaagtaac gattcaagac caactgcttt attttgcaga acttagaggg
   129181 aaatcaagaa aagagattga accaaaaatc gatttttgga tggaaaaatt tcaggtaaaa
   129241 gggaaaaaga cggataaagt aaaatctttg tctaaaggaa atcagcaaaa agtccagctg
   129301 atcgccacgt taatccatga acctaaattg atcattttgg atgagccatt cagcgggtta
   129361 gatccggtaa atgctgaatt gctaaaagat ggaatattgg aattgaaaaa acatggttca
   129421 tgtgtcattt tttctagcca caatatggat aatgtagaaa aaatctgcga tcatttgatc
   129481 atgttgcgaa acggaaaaat ggttttgaat ggaaaagtac atgagattcg tgaatcattt
   129541 ggccgtacga agttattctt ggaatctggt ttaagccaac aggaagtaga agaaatcgaa
   129601 ggcgtgaaaa aagtgatcct tcatgaagac aatacgttag agattacctt agaagatcct
   129661 gaagctggga aagaaatctt tactcgtgcg actcagttcg gctacattcc tatgtttaac
   129721 cagcaaccgc caactttgga agagattttc aaattgaaag caggtgaaat cgatgcgtaa
   129781 gttttggatt attaccaaag atgtctattt aaaaaatgtt aagtcgattt ctttcttgat
   129841 catgatatta gtccctttta tcatgctggg tatcatttat gtagcaggaa actttgctca
   129901 acaaaatagt gatgtcaata aaatcggcgt attggctgat aatgagcaaa tcagtcagca
   129961 aatgagccaa gtcaaaacag atgattttga gttcaaagca gttgattctg aagaaaaagc
   130021 acaaaaagag ctttcggaag aaaaaatcga tgcgtattta gtagtctcaa ctgagaatga
   130081 taagataaat gggatgcttt atagcgaaaa ttcattagga caaagtacac aactactgat
   130141 acaacaacaa ctgactggct tgcaatcgat gcttcgagca agtcaattag gcatttcacc
   130201 agaagaagta gctagcttga gccaacaagc aagtttgact cgacaaaaag tcaattttga
   130261 ttcaaaagga aaaatgacag ttggagaaga taactcgaca gtccaatatg tcgtaagtta
   130321 tgtagcaacg attgtcttgt tcattattat tttgacgtat gcgcagatca ttgcacaaga
   130381 gatcgcttct gaaaaaggca cgcggatcat ggaagtgatc ctgtccagta cgactgcaca
   130441 aaagcatttt tacggaaaac taacaggtgt tttacttgtt gcattaacgc aaatggcatt
   130501 atacggtatt atttttggta taggatacag tcaattcaaa gatatggaaa tggtcaaaaa
   130561 tatattagac ggtatctcat tggataactt gattggacca ttcttgtggt attctcttct
   130621 attcatgatc tttggcattt tgattttttc tgttttagca gctttatgtg gctctttggt
   130681 caacaaagca gaagatacag caaaagcaat tttgccagtg acttatctat ctctaggcgg
   130741 atatatgctt ggattgatac taggagcttc agatccaaac aatatcgtta ttcggatcac
   130801 ttcgtatatt ccattcttat cttcatatat tatgccggtt cgcttagcaa atgagacagt
   130861 cggtatgggt ggagcattga tttccttgtt ggtacttgta gccgtaacat tcttattgat
   130921 gatcttgtct gcaaacatgt acaaatcgaa tgtactcgtg tatagcgaag gcggattatg
   130981 gagttcattg aaacaatcga tttcaattat gagaaatgaa cgaaaaaaag cataaaatat
   131041 agaaaaatat agaaggaagc tggacattta ttgttcccag cttctttttt gtatagacct
   131101 atttctattt ttttattata ttggatatat atattcattg aaaatagagc ataacaactt
   131161 tataatgttg agtataccaa tttttggagg cgttttaatg gaccgctatt tagatttact
   131221 ttcagagaag ttccctacaa cggaagatgt catcacggaa atcatcaatc tcgaagcaat
   131281 tttgcagctt ccaaaaggaa cagagttatt tttgagcgat atacatggcg aatttcctgc
   131341 ttttgatcat attctgcgta tcggttcagg aaatcttaaa gaaaagatca aagacctttt
   131401 ctctgaacaa atgacggaaa cggagatcag ccaattcact atttttacgg cttatccaga
   131461 atatgtgttg acgactgatt ggtacaaaaa acaagataaa tcccagctga tccatcgttt
   131521 gatcgatctt ttacggttta cgacagtcaa atacactcga tcaaaagttc gaaaaggatt
   131581 gccgaaagaa tatagctata tcattgagga actcttgtat atcgatgatc gtatcaacgg
   131641 gaaaaaagac tatgtaaaaa aaatcattga acaacttgtc ttgatgaagg aagaagaaaa
   131701 atttttaaca aaacttgcgc agacgatcca aaaatctgtc atcgatcatt tgcatatcgt
   131761 gggtgatatt tttgatcgag gaactcaagc agccaaagta atggatcgaa tcatggattt
   131821 ttcctctgta gatatccagt gggggaatca tgatattttg tggattggtg cgtattgcgg
   131881 ctctgaggca tgtttattga cgcttttacg gattgccgca cgctacaact atttatttga
   131941 attagagaaa gaatatggat taaatttacg tccattattt tcttttgccc atcaaaccta
   132001 tcagaagaat ccagtattta cccctaaaaa caggaaagat ctatctgaaa gagagcaaga
   132061 agaattagaa aagatccatc aagcactttc tatcctccaa ttcaaatcag aacatcagtt
   132121 gttgaatcga cgacctgaat ttaaaatgga cgatcgtaaa ttgttggaaa aaatcaacta
   132181 tgaggaatca acgatcgttt tagaaggtca gctctatgag ttgaaaggga cttgttttca
   132241 aacgatccag ccagatgatc ctaaaaaatt cttggaagag gaacaaaaag tgatcgatag
   132301 tttgatgtat tcattccagc actcgttaag aatgcaaaaa cacatgacgt ttttgatcga
   132361 aaaaggggga atgtatctca cgaacaatca gcatgtactg ttccatggct gtatacctgt
   132421 agatgaaaag gggcagctct tagcatttga attagaccaa tcttatcgcg gaaaagaact
   132481 gctgggattt tttgaaaaac agataactga aagcgcaaaa gatttgactg caaatgaaga
   132541 tttggcgacc gatttgattt ggtatgcgtg gagcgggcca ttttcgcctt tattcggcaa
   132601 gagtaaaatg gcaacttttg agcgctattt tctcacagag cctcatacgc acgtcgaaaa
   132661 aagcaatccc tattatcact tgcgagatga agagtggttc agtgaaaaaa ttttggcaga
   132721 attcgatgca aaagaagaag gttcagcaat catcaatgga cacacgccag taaaagtaaa
   132781 aaaaggagag tcaccgatca aagcaaacgg aaaagcattt gttattgacg gaggtctatc
   132841 caaagcttat caaaaaacaa ccgggattgc tggctactca ttactatgca attcttacgg
   132901 tttccaaatc gtgacacatt atccgttctt gcctattgaa caacaatttg caaaaaaatc
   132961 tgatcaaacg aatgtaaaaa aagtgatcga gcagcagctc ccacgcaaac ttaatcggaa
   133021 tacgacaaaa ggaatggaat tacagcaaca gattactcaa ctccaaaggc tgttggatta
   133081 tcgaaaggac gattgagaaa aatgctagcc aacattgctc aatagactaa aaagcgactg
   133141 caacaaaagg cgtgtcgcag tctccttcgg aattagctca aaaaacggta aaatattaga
   133201 agctattttt ctgtttattt ctctaatact caggtcaggg cgctgatttc acgacctctt
   133261 ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatcc aacaaacatg
   133321 agaagctgtt ttttgaaatt gctccttgtg actcagagtt gaacggcagg ctcacgacct
   133381 aatcagaaaa acgattaaaa ttcattcaca aagaaatcga ataaacgctg ttcagatgaa
   133441 tctacacgat gagaaagttc cggatgccat tggacaccaa ggattttgtg catattatct
   133501 ttactttgga ctgcttcaat gagtccatcg ttggaaaagg cgatggcttt taacaaagga
   133561 gaaagatctt tgactgcctg atgatgatag gaattcacaa agtatgaatc agggagaagg
   133621 tcacttaata aactgtccga aacgattttg acttcgtgag ttgcaaactg cggggcagtg
   133681 ggctgttgtt cgtgttttac ggaccatttt ggatataatg aaagatcttg ataaagtgtt
   133741 ccctccaaca cgacattcaa tagttgaagt cctcggcaga ctgcaaaaat cggttttctt
   133801 tgtttcaatg cttccaagat caatgcctgt tcgaattggt ctcgttgaat atttgtttcc
   133861 gtcagttttg gatgagggtc ttccatgtaa agttgcggac taacatcctg accgcctgca
   133921 agaatgagtt tgtcgatttt ctcaattagt tgaggcgctg aatctggaga catgactggc
   133981 agaataagag gtaatccttg agcatctaaa actgcatcca caaatccctg cggtgtatag
   134041 cttacttgat ttccttggaa agtatctgtc gctcgaatca gttgattccc ggcaatgccg
   134101 atgattggtt tcatttttat tccccctaaa gtagactaat tgtaacaagg atttaaaaaa
   134161 atttaaacta tgtaagactt gcccctatac ttatctgttc ttgctgtgtt actatctgta
   134221 aagagaattt tcgtgtgaaa ttcatgacga tagttgaaaa tattgcttaa tttgttataa
   134281 taaaaagtaa gcagaaataa aaaggttggg cgtaagcttc aaggatctag ttcttcaagg
   134341 ggtgagaagg acattttatt gaaggggcct gcctatacta agttatcttt agaggtacat
   134401 actttcaatg aaaaaagaaa gcatgtacct cttttttaaa tcgaaaggaa gtgaagcaag
   134461 tgttagaagc tttggaacaa atccgaaaag cagaagaaga aaatgagaag cgtgctcgaa
   134521 ccctacggca gcagttgaag acctatgcag aagaaaaaga gcaagaattg gagataatcc
   134581 aaacgaacag tcggcaacag ctgaaacagt tggtagaaga aaaagaacaa ttcgaaaagc
   134641 aagaattaga aaaagaaaaa atgaaattga aagcagaagc agacaaggtg caagcaactt
   134701 tgaaagacca atatcaaaag catcagagtc aagcaatcgg tgcaatcatt gaaagggtga
   134761 aagaaactta tggccgtcac taagatggaa aaagtgaccc tcatttcaga taaaagaaat
   134821 caggagagga ttttacaagc agtacaagga atccaaaatg ttgaaatccg ggatctgtat
   134881 caaaatagaa caaacaacca gtgggtagaa aaatattttc cagataccac gattattgat
   134941 aaagaagcaa aattaagcgc tttaggcaat cggctgaaag acatccaaga agctatccaa
   135001 ttcatacaac accacggcga tagccgtcaa aagaaactcc atttgaaacg tcaagaactt
   135061 tccttacaag acttggaagc aaattattcc gaagaaaatt tcatgaataa attagaagaa
   135121 gtccttgatt taaaagaaaa gtgggaacag ctaactgaaa aacgtgatcg tttagaagaa
   135181 gaggaagaat ggcttcttca ttggcaacag ctggatattg ctccaagtat gtatgcaagt
   135241 aagacgacca tttttgagat ggggactgtg agtgcagcaa attttgaaag tttcaaggaa
   135301 gaactaagta agcttgatga cgtttatatg gaagaaatag attatacgcc aaaacaagtt
   135361 tatctttcgt atgttgcttt aaaaaaatca gttgctgcta tagaagaaat tgctgggaaa
   135421 tacgggttca ataaagaaac gtatccttat gaacaaaatc ctaaagaaaa attgcaagaa
   135481 accaaacagc aattatccat tgttcacgag cagcaaaaaa agataacgac agcgttaggt
   135541 cagtgcagcg gctatatcca agaatttgaa tgggtagaag aagtcacact tgccgttgca
   135601 gaacgggaaa aagtaaaaga acggtttatc catgccactt atctgatcgt tttgcaaggt
   135661 tgggtagatg cagaagaaaa acagaatttg atgcatgtgt taaacgaaac tgtatcagaa
   135721 gaagatatat acgttagttt cgaaacacca aactcagaag agattcagca ggaagtccct
   135781 actaaattga aaaaccatcc gcttatcgaa ccttttgaat tgctgactga aatgtacagt
   135841 ctgccaaaat atgaagaagt agatccaacg ccttggatga caccatttta tcttgtcttc
   135901 ttcgggatga tggtcgcaga tgttggttac ggattgctga tgctcattgg gacaatacta
   135961 gctcaaaaac tatttgtttt accccgcggg atgaaacgtt tcgtaaaatt ctttgagatt
   136021 ctctcggtac catcgatcat ctggggattg atctacagtt catttttcgg acaggcatta
   136081 ccaaaagaac tatttggtat ccgcttgcct ttcccaatcc tatctacgac ggatgacgtc
   136141 aatacgattt tgattttatc cgtgattttc ggtctgatac agatcttggt tggattattc
   136201 gtggcagcaa aggaaaatat caggaggaaa gattacttga gtgctgtcag tgatggtttt
   136261 gcctggcaag ggatcctgat tgggatcgtt attgcattga ttggtgtcat gttgataaaa
   136321 aatacagcat ttgtttatct tggcgcgggg ctatcgatca ttgctgcttt atgtatctta
   136381 atcgtgccga ttatccaatc gccgtctaaa gtcaagggtg ctgccaaagg agcgtataat
   136441 ctttacggat tgactggtta tatcggtgat ttagtcagtt atacacgttt gatggcacta
   136501 ggtatttcag gcggaagtat tggcgcggca ttcaatatgc tagttgcttt catgccgcca
   136561 gccgctcgat tcagtgtagg gatccttttg atcatcgctt tacacgcact gaatatgttt
   136621 ttgacactgt taagtgccta tgtccatggt gcacgattgc agtatgtcga attttttgga
   136681 aaattttata caggaggcgg acgtgccttt gacccgctga aaacggcgga aaaatacgtc
   136741 aacatcaatc ataagaaaaa aaacaataaa taattggagg atttttatac aatgatggat
   136801 tacttaatta gtcaaaatgg tggaatggta tttgcagtat tagcgatggc aacagcgacg
   136861 attttttcag ggattggttc agctaaaggt gttggaatga caggtgaggc agcagcagcg
   136921 ttaacaacaa gtcaacccga aaaattcgga caggctttga ttctgcaatt gcttccaggt
   136981 acgcaagggt tgtatggatt cgttatcgcc tttttgattt ttatcaactt aggaaatgat
   137041 atgtcagttg tacaaggatt gaactttcta ggcgcttctc tgccgattgc ttttacaggt
   137101 ttgttttcag gtatcgccca agggaaagtt gcagcagcag gtatccaaat tttagcgaaa
   137161 aaacctgaac atgctacaaa aggaatcatt tttgctgcga tggttgaaac atatgcgatt
   137221 ttaggtttcg ttatttcctt cctattagta ttgaatgcct aagcataaag gaggaaaagc
   137281 agatgaacgc cattgaaaaa atcatctctc agatgaatga agcagcagag caagaaagag
   137341 cggcgttaga gcaagaagag cgaatgaaga tcgatcaaaa ctttgaacaa aaacgtacgc
   137401 aaatcgaaac agaacatcaa aaacaaaaag aaaaacaaat cgaattactt gaaaaaaaat
   137461 accgtcagct taggaatcgc caacaagtag aagtacgaca agaaaatttg aatgcgaagc
   137521 aggaattttt acgacgattg tttgcagatg caatagctga aatggagaat tgggatgaat
   137581 ctgaacaaat ccagtttatc aaaaattcat tgtattcatt acctttgaca ggcaaagtaa
   137641 cattcattgc tggagaaaaa tcagcagctt atctttctca gacattgctg gatgaatgga
   137701 acaatgaact tccctttatg atggttttaa gtgatgaaac tgttgctgat caagcgggtt
   137761 ttctgataaa cgatcaagga gttcagtata attttctttt cagcagcctt gtacaagata
   137821 tccaaggaac gatgagtttt gagattgcca atcaattatt cgaataagga gggatcagtg
   137881 tatggaatac cacgaactaa atcccttgat tcgcggaaga gagcttgaac ttttgtccaa
   137941 tgagacattt gaacggatga ttcaaacaaa ttctgtcgaa gcacttggtg aaatattgaa
   138001 atccacgatt tacagtccat atatttatga cggatatgaa tatgacttcg aacagaatct
   138061 tttaaaggag cagagccgtc tatttcgctg gttaaaggaa tcagcaccgg agcctgaaat
   138121 cgtttggatc tatacgatgc gctatacttt ccataatctg aaggttttga caaaagccga
   138181 gatgacaaat caaaatctgg accatttgta tatcgacgat ggattttatt cattagaaac
   138241 cttgaaggat gccattcata cgcaagtttc aaatgaactc cctgaaagca ttatggacag
   138301 tatacgagaa gtacgtgatt attttgaaga gtctaccatt cttcagggaa ttgacgtgat
   138361 ttatgatcga cagtttttga cagagcagcg acggcttgcc gataagctag gatatcaaga
   138421 attgctgaca gaagtcattg cttttattga cttgaccaat atcacaacga tggctcgagg
   138481 aatattgcag aaaagaacat atgggttcat gacgacggta ctttccagct cgggaagtat
   138541 cgaaaaagaa gtatttttga actttgtaga taaagatatg gaagattaca cgaattttct
   138601 attagagaca gattatgctg attttctgcg ccctgtcatc cgtgagggaa atcttgatct
   138661 cgtaaagctt gaacagttga aagatgatta tctaagttca ttgtatcaag aagcacaaac
   138721 ccaggctttc ggaccactgc cactactagc ctttttgaac gcaaaagagg tagaaagcaa
   138781 aaaccttcgt ttactggtta tcggcaaaag aagccatttc gaaccagaaa caatcagaaa
   138841 gcgggtgaga caagtctatg acgcataaga tcggtgtagt tggcgacaaa gattctgttt
   138901 tgccattcaa attgtttggc tttgatgttc gttatggtac aaccaagcaa accgttcgtc
   138961 aggcaattga agaaatggca aagaatgagt atggcgtgat ttatgtcaca gagcaatgtg
   139021 cagcattgag cccagaaacg atcgaacgtt acaaggaaca aatgatacct gctatcgtat
   139081 tgattcccaa tcatcaagga tcattaggga tagggctgga agaaattcaa aaaaacgtag
   139141 aaaaagcagt aggtcaaaat atcttatagt atgaaaagga gatagaaatt ttgcaaattg
   139201 gaaaaatcat aaaagtctcc ggacctcttg taatggctga gaacatgtct gatgccagta
   139261 tccaagacat gtgtctagtg ggagatctag gagttatcgg agaaattatc gagatgcgtg
   139321 gcgatgtggc gtctattcaa gtatatgaag agacttctgg aattggtcca ggcgaaccgg
   139381 ttcgctctac tggtgaagct ttatctgttg aattaggacc agggatcatt tcacaaatgt
   139441 ttgacggaat ccaacggcca ttagatacct ttatggaaat cacacaaagt aatttcttag
   139501 ggcgcggagt tcaattgccc gcattggacc atgagaaaaa atggtggttc gaaccaacag
   139561 tagaagccgg agaaacggtg agtgctggag atgttatcgg gatcgtagaa gaaacaaaag
   139621 ttataaaaca taaaatcatg gtgccaaatg ggataaaagg aacgatcaaa acaatcgaaa
   139681 aaggttcatt cactattgat gaaacagtat gtgtcattga aaccgaaaat ggtgacaaaa
   139741 atttgacgat gcttcaaaaa tggccagtaa gacgtagtcg gccaatcaaa gaaaaattaa
   139801 acccggatgc acccatgatc actggacagc gagtgattga tacattcttc ccagtgacaa
   139861 aaggaggagc agcagctgta cctggtcctt ttggtgcagg gaaaacagtc gttcagcatc
   139921 aaattgcaaa atgggccgat gtggacatgg ttgtctatgt gggatgtggc gagcgtggaa
   139981 atgagatgac ggatgtttta aatgaattcc ctgaattgat tgaccctaat acaggagaat
   140041 cgttgatgga acgtacggta ttgattgcta atacatcaaa catgccagta gcagctcgag
   140101 aagcttctat ctatacggga atcacaattg cggaatattt ccgtgacatg ggttatgacg
   140161 ttgctatcat ggcagactcc acttcacgat gggcagaagc acttcgtgag atgagcggac
   140221 gtttggaaga aatgcctggt gatgaaggtt atccagctta tcttggttca cggcttgcgg
   140281 aatactatga acgatctgga agagtcattg cattaggatc agaaggtcgc gaaggaagta
   140341 taaccgcaat cagtgctgta tccccgtctg gaggggatat ttcggaacca gtcacacaaa
   140401 atacattgcg ggtcgtaaaa gtattctggg gtcttgattc cagtttagcc caaaaaagac
   140461 attttccttc gatcgattgg atacaaagct attctcttta ttcaacagaa gtagggaagt
   140521 atatggatca tatccttcaa gctgactggg catccatggt caccgagggc atgcgtattt
   140581 tgcaggaaga acagcagctg aatgaaatcg tgcgtttggt agggattgat tccctttcag
   140641 ataatgaccg tctgacatta gaagtggcaa aatccattcg tgaagattat ttgcagcaaa
   140701 atgcttttga tgatgtcgat acattcactt caagagagaa acaattcaaa atgttgaaga
   140761 tgatcttgac ctttggtgaa gaaggacgcc gtgcgttgtc gttaggtgct tatttaagtg
   140821 agatcatgga cggtaccgta tcgattcgtg aaaggatcgg acgaagcaaa tatatcccgg
   140881 aaacagaatt agaaaaacta gatcaaatta ttggtgaaat caagcaaacg atgcaaacga
   140941 tcgtttcaga aggaggcatg agcaatgatt aaagaatatc gtacgatcgg tgaagtcgtc
   141001 ggtccattga tggcggttga aaaagttgct ggtgtcaaat atgaagaatt aatcgaagtc
   141061 cgtatgcaaa atggcgagat tcgtcgtgga caagtattgg aagtccaaga agataaagca
   141121 atggtccaaa tattcgaagg aacaagtggt atcaatttac gtgactcttc tgtacgattt
   141181 ttaggccatc ctttagaact aggtgtatcg gaagacatga tcggtcgagt attcgatggt
   141241 ttgggacgac cgaaagataa cggaccagag atcctgcctg aaaagcattt ggatattaac
   141301 ggagaagtaa tcaatccagt agctcgggat tatccagatg agtttatcca aacaggtatc
   141361 tctgcaatcg atcatttgaa tacattagtt cgtgggcaaa aattaccagt attttctggc
   141421 tctggtcttc ctcataaaga attagctgct cagatcgcgc gacaagcaac ggtactcgat
   141481 tcatcagatg atttcgccgt agtctttgct gctattggaa tcacttttga agaagctgaa
   141541 tttttcatgg aagattttcg tcaaacgggc gcaatcgacc gttctgtcat gtttatgaat
   141601 ttagcaaacg accctgcaat cgaacgtatc gctactcctc gtatggcttt gactgctgca
   141661 gaatatcttg cttatgaaaa gggaatgcat gttttggtta tcatgacaga catgaccaac
   141721 tatgcagagg cattgcgaga gatttctgca gcacggcgag aagttccagg aagaagagga
   141781 tatcctgggt atctttacac gaacttagct actttattcg aacgagcagg aagaatccgc
   141841 ggactaaaag gatcggttac tcaaattccg attctaacaa tgccagaaga cgataaaact
   141901 catccaatcc ctgacttgac tggttatatt acagaaggac agatcatctt gactagagag
   141961 ctttacaaga gtgggattca gccaccaatt gatgtactcc cgtctctttc gcggttaaaa
   142021 gataagggaa ctggacccgg aaagacaaga gaagaccatg cagctacgat gaatcagctt
   142081 tttgcagcct atgcacaagg aaaacaggca aaagaattag ccgtagtttt aggtgaatct
   142141 gctttatctg atgtagacaa gatctatgcg aagtttgctg agcggtttga aaaagaatat
   142201 gtcaatcaag gattctacac gaaccgttcg attactgaaa cacttgatct agggtgggaa
   142261 ctgctatcga tgttgccaag aaccgaatta aaacgaatca aagacgacat gctggacaaa
   142321 tatctgccag aagggaagtg agttctgatg gcacgattga atgtgaatcc gacccgcatg
   142381 gaattgactc gattgaaaaa acagttgaca actgccacaa gagggcataa attattaaaa
   142441 gataagcaag atgaactgat gcgtcaattc atcctgttga ttcgaaagaa caaccagctg
   142501 cgtcaagaaa tggaagagtc tatgacagaa gcaatgtctg attttgtatt ggcaaatgct
   142561 tcgatcacgg aacctttcat cgaagaatta tttgttttgc cggcggaaaa cgttgatttg
   142621 tctatcgtag acaaaaatat catgagtgta aaagtacctg tgatgaattt tgaatatgat
   142681 gaacagctga cagacacacc tttggaatac gggtatttga attccagtgc cgaactggat
   142741 cggtcaatcg atcggtttac taccctgctt ccaaaacttt tggaactgac agaaatcgaa
   142801 aaaacttgtc aactgatggc gagtgaaatc gaaaagacac gtcggcgagt caatgcatta
   142861 gaatatatga cgatccctca attagaagaa acgatctatt atattcgtat gaagctggaa
   142921 gaaaacgagc gcgcagaagt aacaaggttg atcaaagtaa aaaatatggg taccactgac
   142981 taagtggtat ctttttttaa aagaggaaaa cgatgattaa acaaagcaaa caaataaaga
   143041 aacgtttgcc ggctgttcag cttctcgcat taggattttt tctcctgatt ttagttggcg
   143101 gcggcctact taccctacca ttttttagta atagtgggca tgggacaaat tttatcgatg
   143161 cgcttttcac tgcaacttct gctgtctgtg taacgggatt gacaacattg aatacagcag
   143221 agcattggaa cagtgctggt caatttttga tcatgctttt gattgagatc ggcggtcttg
   143281 gttttatgat ggttccgatt ctatgctttg cattgataaa gaagaaagtc agtctcagca
   143341 cacgtattat attaaaagaa gcgctgaatc tagaagaagt atcaggtgtg atgaagctca
   143401 tgctttacat cctaaaattg gcctttgtta tccaattgat tggtacggtt gctttatctt
   143461 ttgtatttgt gcctgaattt ggctggttga aaggtatctg gtacagtttg ttccatgcaa
   143521 tctctagttt ttgtaatgca gggtttgatt tgttaggaga cagtttagcc aatcaccagc
   143581 aaaatgttta tttgatcatg gtggtatctg ctttgatcat ttctggtggg ttaggcttca
   143641 tcgtatggcg tgatttactg gaatatcatc agaaaaagaa gatgacgatt cattcaaaaa
   143701 tcgcattaac cgttacaggt acccttttat taggcggatt cgttgtgttc tttttcacag
   143761 agcgcaatgg ggcaacgcta gtggaaggga atatgtttca acgattggca aacacctttt
   143821 ttatgagtgt gacacctaga acggctggat attattcagt tgattacctc catatgagcc
   143881 atgcaggtct gattttaacg atgtttttga tgtacatcgg cggaacatct gggtcgacgg
   143941 ccggcggatt gaaaacaact acgttaggtg tgttgctgat ccagatgcgt tcgatgttga
   144001 aaggacgtac gagagcggaa gcatttggcc gtaccatccg acaaggcgca gtgttgcggg
   144061 cacttacttt attttttgtt acgttgacgt tgtgtgtagt cgccatcatg attttatcgg
   144121 tgactgaaac gatacctaaa acttctggca tcgaatatat tgcttttgag gtcttctctg
   144181 cgtttggtac agtaggtctt acgatgggat tgacgccaga cttgactgca atagggaaat
   144241 tgatcattat agcgcttatg tacattggac gagttggtgt attgactgtg gtcttctctc
   144301 tgatgacaaa agcacaaaga acacaatcaa aatacaaata tccagaagaa agtgtcatga
   144361 ttggataatt tcatcttctg acagaaacct agtgatttag taaaataaga aaataaaata
   144421 atttttcttg cttcttggat actttaagtg caaaatgttc gtttattttt tgaggcacta
   144481 cccgttgttg aacctcgggt agtgcctctt ttttattgaa aggaatcaat aagatttgat
   144541 tcctttattt atcaataggt tataattcat atttttttat tttttctttt acgatactta
   144601 gtccagaagc taaaatttca aggttattag aggataggga aattcgaacg aatgcatctg
   144661 gttgcgcacg tgtagtaaag cgtctggaat gataaagtcg gataccgtta gcaagaaagt
   144721 cattctcaat tatcttaggg tcaatctcag gatcaactgg aagaattcga tagtaaggga
   144781 cacgattcgt tggtttttct actttgaaaa gagaatcaaa aagctgattg gctctttttg
   144841 taagacttaa tttagtttct aagatcctat ttgcttcatt tgaatggagg atttgagtga
   144901 tgacttccac atctaatcca gaagttttca catttatatt aaaaattgct tgttctatct
   144961 tgctttggac atgtactgga actacaagat aagcaactcg caatccagaa caaataaatt
   145021 ttgtcattcc agctaagtag attgtttggt taggtaataa attttggaaa ggaggaatga
   145081 tcgtatgttg atagtaagtt gttaaaaatg aatggatatc atcttcaata acccatagac
   145141 cttctttttc aatcacttcg gctaattgct ctcgtctttt gtgagagatt tgaaacccta
   145201 taggattatt acaagaaggc ataagaaaga caccatgaat cttctttttt ctacattcct
   145261 gaatcaatat atcaggtcgc attccttgct cgtcaaaatc aattggaacg atctctaaat
   145321 gatataactg agccaattca atgaaattag cgtatgtata tcggtctgtt gctattctat
   145381 cacctggaga gaaaagtgca gaaagtgtga tagccaatcc attttgaaca ccggaaacaa
   145441 tagatagaga ttctaaggac ggaatgactc cttgagtttt taaccaattt gctgcagtag
   145501 tcaattgaaa atttgtgcct ttgggatctt ggtaattaag caaaccaaca agatttgggt
   145561 tattactagt tgattgaata aacgggacaa cgaatttatt acattcttca aaagaactaa
   145621 ccagaccaaa atcaatgata tgttcggtta cttcattggt agagatggtg atggactcaa
   145681 agacattggg agaaataaat gtgccacttc caatattcgt atacagcaaa cctttctcaa
   145741 ttccatattt ataagccttg ccaacggtag taaaatttaa atctaaataa tcagctagct
   145801 ctctttggga gggtaactga gtgtttttct gtaatttatt ctttaaaata tcttgttcta
   145861 attgttgaat caacgatcga taataaggac gcttcaattt ttgtttgtct ggtcgccaag
   145921 ttaacgggta attgtcataa gaattaatag gcattttctc cactccttaa aattgtatac
   145981 catacaattt taccattgat tgtattgatt ttagaagtta atcttttgtg aggagggaat
   146041 tgtctatgaa aattattaat tttgaggaag tagtagcttc ttttagtttt gaagaagcga
   146101 ttcaagtaat gaaaaaatgt ttttttgatt atgaaagtgg taaaatcagc caaaatcctc
   146161 gattggtgga aattttacct gacggaaaag gcaaaaatat gtttgcgatg atgcctgctt
   146221 atttggggga gaacagatat tttggcacaa aagtcattac cgcttttccg gataatcatc
   146281 agtatcattt accttctcat ttaggagaga ttttcttgtt tgatgctcaa aacggactac
   146341 ccaaagcatt gataaacgca aatgcagtca cctggattcg aacagctgct gtatctgcgc
   146401 tggcaacaga ttacttagca aatccgcatg cagaaacact tgctttaatt ggagctggtc
   146461 aacaagcatt gtcgcatttg actgcgattt ctaccattcg tcccatcact acagtgtttg
   146521 tttacgatat tttagaagaa agacgaaata cttttatcgc taaagctaaa aaacagtatc
   146581 ctaaaataac gtttataaat gcaatatcag tagaagatgc agtacgtcca gcggaaatca
   146641 tttgtacgtt gacctctagt aaagaggcat ttcttcaaaa agagtgggta agggaagacg
   146701 cacatatcaa tgctgtgggc acatttactc ctgatacaag agagataagt acatcgttga
   146761 tggaaacttc tcaaatctat gtagatgatt actcagctgc cttaacagaa agcggggacc
   146821 tgcttatagc catggcagaa ggagatttag aggaatcttg tattgttggt acattaggcg
   146881 aattagtttc tgagaaaaaa acattttata aaggagaatc gaggccgacg attttcgacg
   146941 cagttggtct agctattgaa gatctttgtt gtgcagaata tatttataac aaattagaga
   147001 aaggggcttt aaaatgaact tagattatta cgtagttgat gcatttgcag atgaggtgtt
   147061 caaaggaaat ccagcagcgg tttatgtgct agaagaatgg ctgccagaag gtacgatgca
   147121 aaaaattgcc attgaaaaca atttatctga aacagcattt acagtgaaga aaaaccaaga
   147181 atttgagctt agatggttta cgccagatcg agaaatcgat ctttgtggtc acgcgacatt
   147241 agcaacagca tttgttttat ttaactatta caaaatacct gatgaaacga ttaaattttc
   147301 tactcaaagc ggtaatctat ttgtgacaag aattaaagat tattattaca tggattttcc
   147361 tagtattatg ccgaaaaaag tgccgatatt gtctgaatat gaagaagcga ttggggctaa
   147421 gattaaagaa gcctacttag cacgtgattt atttttcgta ttagaagatg aaaaaacggt
   147481 ggctaaattg cagccagatt ttacagctat caaaaatttt gaattaggaa taggagtcat
   147541 cgtgacagca gaaagtgtgc aaaaagactt tgtttctaga accttctttc caaaattaac
   147601 aatcaatgaa gatccagtat gtggatcagc acattctaat cttattcctt attgggcaga
   147661 aagaatgaat aaaacaaaat tgtcagctta tcaaatgtca tcaaggggcg gggatctaga
   147721 atgtgaatta aaagaagatc gtgtgataat aggaggaaaa gcagttttgt tttctaaagg
   147781 tacctcgttt atttgatttc tttcaaatga attaaaaata aattggtgga gagtggcaac
   147841 cttatagcta caatggttgt taaaaaaaga cgcaaacttt ttcaatttaa tgaaaagttt
   147901 gcgtctcatt ttaattattg gtgtttgttt tttattcaat caagagtaaa atgccccata
   147961 ctaactatcc tcggatgact tcgattttat aaccatcagg gtcaatgaca aagtagtaag
   148021 aaggcgcagt gccaggtaat ccttttaagt cagtaatctc aagaccggca gctttatgtt
   148081 gctcgtgtaa tttttctaga tcatcagctg ctatagcgat atgaccatag ccgtctccta
   148141 gatcatacgc accatgatca tagttgtaag tcaactctaa ttcatagtca tccccaggaa
   148201 gtgtcaaata aacaagtgta aatttgtttt ctggaaaatc acggcggcgg ctttcttcaa
   148261 aaccaaatgc cttttgataa aattcgattg atgcttcaag atctttcact cgaacacatg
   148321 tgtgagccat tttcatagat tcatctctcc ttttcctttt actcaatcat aacaaaaaga
   148381 agtaaggaaa tagttgattt gtgcttgttc atgagaaatt tctcatgtaa aatggataga
   148441 aaataaaact gttaaggtgg aaactattca tggataaacc aatttttggt gaaaaaaagc
   148501 aatttgctga ttatcaaacc cgttatgcag catatattgt catagaaaga gatcagcaga
   148561 ttgcagtaat cgaggctcca aatggtgctt tttttcttcc tggcggggaa attgaaggag
   148621 aagagacgaa agaagaagcg attgtccgtg agctgataga agaaatggga attgtggcag
   148681 aaatttcttc ttatctggga gaagctgatg agtattttta ctcgaattat cgtgcaactt
   148741 attattataa tccaggatat ttctttgttg ctgattcttg gaagcgtata ggagaaccaa
   148801 ctgaaaagac caataaaatt tggtgggtga gccctcaaga agcgcttgtg aaattgaaac
   148861 gtggtagcca ccgatgggca gtcgagagat ggatcgaaaa aaataaaaac tgaaagaaag
   148921 cgagtagatc agctttctct cagttttttt atttattaca gtacatattt tttgattgct
   148981 tcagctacac cgtgctcgtc gtttgatgaa gtgatgacat ttgctgcttc tttgacaaga
   149041 gaaacggcat ttcccattgc tactcctaga ccagcatatt caatcatgga taagtcgttc
   149101 tcgttatctc cgatagccat gatttcatct tgatcaatac caaggtgctg tgcgagattc
   149161 gcaactgccg cacctttact tgcttcttta tttagtactt cgaagtaata gggcgcactt
   149221 ttgaccgttg tgtatttatc acggaaagtt tgcggtaaac gcgcaattgc tgcatctaaa
   149281 atttctggtt cgtcgatcat catcattttg acgatgttca tgtctggtgt catttcttca
   149341 ggtgttcgat attttagcgg catcttgact aaacttgctt cgtggatcgt ataagcgctg
   149401 atatctcgat taggcgtgta gatcgctttg tcatcgatcg aatggagatg actgccgaca
   149461 cggcgtgcca tcacttcgat ctctaggaaa tcttcatgtg tcagtccgtg acgggcgatg
   149521 attttacctg atttcgtttg ttgtacgagt gccccgttat aagtaataac gtaatcatta
   149581 tcttgaaaaa ggttcaactc gtttaactgt tcctggacac ctggtaaggg acggccagtg
   149641 cataaaacga tcgaaacacc ttttgcaaca gcagcggcaa tcgcttcttt tacttctttt
   149701 gttacttctc ttttctcatt taataaagta ccatcgatgt cgatagcgac tagtttgata
   149761 gacataattt cctccaatat attttaattt atttcaatca atgcgccatt acggatatgg
   149821 ctggcaaatt cttgataagt ttcgtcaaat aaatcataat gatcacgcaa ggacggatca
   149881 accatttctt ttggaaagaa aaatcgctca tctccttgtt cttgtcccgc taatgcagca
   149941 acaagctgac taacctgtga taattctatc aaagttccgt catttcgcaa aatctcgatt
   150001 tgtgtgcgat gtctgccttg ttctgggcga taaaaatcat aaggtagatc gtagctagaa
   150061 ttcacagcag tgtaataaac aggattatat ccgacctttt ctactaacaa agtgagttct
   150121 tggactaatt ttgaatcccg gttgccagaa aaagtagcag atttcagtgg tttacgattc
   150181 aaaaaacgtt tagctaaatc atttaaaata ggatcagcag attcagacca ttgagtaaag
   150241 taagtgccca agaccccatc atcaagtttg aggtattctt ctaaagtaaa gcttccgtca
   150301 agaaatggca gaagtaattg agaatggagt tcaaaactgc tggcatcttc ttgatacaac
   150361 tcgtgtgcgc gatgaagcag atgctccaaa attacttcca taccacggga aacaggatgg
   150421 aagtacactt gcacatacat ttgatatcgg ctaacgatat aatcttccac tgcatgcatg
   150481 ccactcatgg aaaaagcgat cccgccttca taaggacgaa tcacacgtaa tattcgggta
   150541 agatcaaatg ttccatattc tgttccagtg aaataagcat cccgaagcaa atagtccatt
   150601 cgatctgcat cgatctgact agaaatcatt tggacaactt gtgggttggg gtaagttttt
   150661 tggatcacac tagctacctt ttcaggaaaa ccttcttcta ctcggttaag aatctgatac
   150721 acttctgttt ctggagaagt gatgatctct acagtgatgg cttcatgatt cgtatggaag
   150781 atatgttcaa atgtatgaga gtaaggacca tgaccgacat catgcaacag tgccgcacat
   150841 aaagtcacca gccgttcctg atcatcccag cctccgttgc ctattttttc ttttgaaaaa
   150901 tttcgagaaa agatatcgca gattcgtctt gaaatctcat aaacgcctaa tgaatgagta
   150961 aaacggctat gttctgctcc gtgaaaagtg aaagaagaag tgcctaattg tttaatgcgt
   151021 cgtagacgtt ggacttcttt cgaattgatc aaatctaaga ttacttgatg ctggacatgg
   151081 atatagttat ggactggatc acgaaaaact ttttccatag gcagcatttg atatttataa
   151141 ggggttgtca taatgtagct cctttatgtt attacgttgt tccattctct ggatttggtt
   151201 gttccattgc tccagctgga tttctttttt tagccagtcg gtagaatcct gtaaatcact
   151261 tgcagaatac agcttttcaa aagcttttgc aaatgcttct ttgacttctt ttacaaaaag
   151321 gggttggtca agcagttctt caagtgttgc cattgtagca ggatccactg caggatagcc
   151381 atttgtacca aagtcttctt ttaaagcaga ttgataaaat cgcttcatga cttgcccgcg
   151441 tttttcctga ttaccgttga cacttaagta catcataaca gaaatcccat ttttgatccg
   151501 acgctgagcg ataccggcaa attttttgcc atggatgctt aaatcataag ttccaggaca
   151561 ataagaatgg gtgatttcga atgcttgtat cggtgccttt ggaaaagcgt atttagtcaa
   151621 taggagcatt tgctcgtaag cgtcatctat ggttattttt tttgtgtcac tttgggggaa
   151681 aatcaaagaa atattcaaaa ttccttcatc tgatacgatc cctaatccac ctgaatttct
   151741 gatcactgga gcgtaacctg catctttcag tgtttggatc ccgtcactta gaaaaggagt
   151801 tcgaacatcc ttcataccta agatgactgt gcagtcatat tgccagaaat gtaaaaagat
   151861 ttgctggtgt tctttagaat attccgttaa tatatctgtt aatgcaaaag gggaaaagtc
   151921 gtttttttga taaatgtgct gatctagtcg aatcgcaggg ttcattcaac cggctccttt
   151981 gtataaattc atattttcta caatattata ctctaaaatc aaagagttgt tttggaaaaa
   152041 aggttttctg taaaaatgga caagttggtt gacaaaacaa aagaaactag gaacaatgta
   152101 gctatgagaa gtaaaggaga atgaataatg gaattatcac aaggaacacc gatttcaatc
   152161 aaattgcgta caaaagtaaa acagcaaggg gaagtgcaag atttttattt tgatttgaaa
   152221 gggcaaatgg tgacaattgg cgatacgctg tacatacgtt ataaagagat tcaggaggag
   152281 acgggcgatg agattcctgt aacgatcaaa ttagttccag atggtcatgt acagctgatc
   152341 cgtgcaggag aaatgcggat gcgtttgaaa tttggctata aagaacggtt agaaacatcc
   152401 tatcgaacac catacggtat gatccagatt gctactttta cgaaagaact gcatgttagt
   152461 ttgaaagacc gtcccacagg agggaaggtt cgaatcgatt atgatctgtt catgggaccg
   152521 gaaaaaatcg gcgaatatta tttgaccttg gattttacag cgtaaatata aaagaaaagt
   152581 ttgatttttt gtgtctgatt ttgtatgata aggagtagac tgtgaaagga cgtgtcctga
   152641 ttggaaatta gtgtatttga aggtgcaaac aaaagcgaat tatcaatgat cgaagtagca
   152701 cacgcgatct tagaacaacg tggcgatgtc atggattttt ctgatttagc taaccagatc
   152761 caaaactatc ttgggaaatc agacagtgaa attcgtgatt cactcgcaca attttatact
   152821 gacttgaata ttgacggcag ctttatctca ttaggggata atcgttgggg cttgcgttca
   152881 tggtatgcta tcgattcgat cgatgaagaa gtaaaccatg gaatggacga agaggatgaa
   152941 gatactccac gtcgccgtaa acgcaagaaa gtcaacgcct ttatcaatga tgatgaagac
   153001 gcgatcgatt acaatgacga tgacccagaa gatacagatc tgacagaaga agatgacgat
   153061 gatctgtttg atgacgatga tgatgaagat gaagaaatcg ctgcttataa ttctgacttg
   153121 caagaaatcg gagcagatga cccgacagat gatgaagaag acttgccagg aaatatcgaa
   153181 gaagatttaa gtatcataga cgaagacgat gatgatgaag attatgaaga ggaagaattt
   153241 tctgactctg aaaaagaata aacattattt tcttacgaga ggttgtgaca gagacgatca
   153301 gcctatcaga ataaggaggt gtgacacgat ttttgtcaca ccttcttttt tgtatataaa
   153361 aatcccaagt aaatacataa aaaataaaaa aattgaaact tttagcgcac cttgtacgtc
   153421 atattaagtg aggcagaaag gagtggagaa tgatttttac tggatataag aaaaaacggc
   153481 agatcgaaaa ataccgtcac ctgctgaatg gactcatcga aactgattat caaaaaatgt
   153541 tccgtatcac gatgaattac gtacatgata aagaagaggc cttagatgtg atgcaagata
   153601 gttttcataa agccctgaac tattttgaaa aagaaaaaga gatcgagcat tttagtgcat
   153661 ggttttatcg gatattgatc cgcacggcat tagatagttg gcgcagacaa aaaaggacgc
   153721 ctgtggactt atttgaaatt caagatatcc cccaacagtt acctttagaa acttccgttg
   153781 cagagctcca tatgatttta gataaaatag acagtccgga aaaagagatt ctgatcctct
   153841 acttttttga aggatttcgt ttgaaagaaa ttgcgactat cttggatatg aatgaaaaca
   153901 cagttaaaac gaaaatgtac cgctcattga atgctttacg tcaaatccta gaatgaagga
   153961 gagaagagtg ccatgtattc ccaaaaagaa aatttaaaaa aactaaagaa aacttataaa
   154021 gaacaagcaa ttcccaaagg gcttaaagag gagatccgcc agcgttttat tatcgaagag
   154081 caatcattta taaaagcaaa gcggagaaag agacgtctcc aagggttcgg agtatctttg
   154141 gctatcgtaa tgattacaac aagtagcctt ctttttaata gtcaagttcg aagctttgca
   154201 gaagatttac ccatcttagg atcagtcatt gaattgatct taggcgaacg ctttactgat
   154261 cgatctgaaa aaatcgatat tcaggtaccg aagatcaata cacaaaatga gaaagaaaat
   154321 aaaacaattc atggattaaa tcaaaaatac tttcgcgaag gacaagcaga ttttgaaaaa
   154381 gctgaaaaag aatatggaaa ttttgaaacg gatcattacc aagttttggg agattatcag
   154441 aaaatcgtag acgataaccg gtttctcgtg attgaaaggc agatcaccca aacagcagca
   154501 gatagccatg ttgaaaaaag atacgacaca atcgacaaga aaaacagtgt ccagctctca
   154561 cttccacttt tattcaaaga tgacacctat ctatctgttt tgacaaaaga agtcaaacgg
   154621 cagatggccg aacaagtcaa ggaagatcca tctaagtact attggactga gcaagatatt
   154681 caagaaggga cgatagaaaa accaacactt gttacgccta cacgaagttt ttatttgaac
   154741 aaagagcatc aattagtttt gactttttct caatacgaaa ttgctcctgg ttatatgggt
   154801 acaccagaat ttgtcattcc taagtccgtg acaaaaacga ttttggcatc ggaagattat
   154861 ttggatcatt agacgagaag tagttgcgac tagcagtttc aattttcaag taagtacaag
   154921 taaataataa gaagagaaaa agagaaagaa gagatatagt gaaaagaaat tatttagtta
   154981 tcagtattat tgctttatcc gtgcttttgt ttttcgaaat tacttcggtg gcctccttga
   155041 tcaccgaagt aaaaggacaa acacactctt cggagcattc gtcacaatct ttcaaacaat
   155101 cagaacaatc aaccattaaa agccaatcaa ctgaaacagc taacgtcaaa gaagccattc
   155161 aagaatcagc ggaaaaggta caaagtcagt catcacaatc aacacaagac agtacaaaaa
   155221 cgagccgaga tccggctgtc aaaagcgtcg caatcagctt tgatgatgga ccaggagcga
   155281 ccacaacacc gcagcttctg cgaatcttaa aagaaaaaaa cgtgcatgcc acttttttcg
   155341 tcttaggaga aaatacggcg cagcatcctg aaatcgtgaa gcagacagct gaagcagggc
   155401 acgagattgg caatcacaca tacgatcatc agaatctggc cactctttct gcccagtcga
   155461 tgacggagga agtgacgaaa gcagacactg agatcaaaaa agtcactggc aaaaccccca
   155521 cttttgttcg tccgccctat ggttctgtca cgaatgtagg agcaacgatc atccagcgac
   155581 cgatcatcga atggtctgtt gattcagaag attggaagac aagaaatcct gatttgattt
   155641 tgcagaagat ccaagcaaca gtatacgatg gggcaattat tttattccat gatatctatc
   155701 ctgaaacgat tcgtgccgtt cctcaagtga tcgattatct gaaagagcaa gggtatcgca
   155761 tcacgacagt tggggattta ttagggcatc caacggctgt cgagaactat tatggcagaa
   155821 acgatcatcg gccagttcag taagtgagac aagtatcttt aataaataac acattttaat
   155881 aagtagttga acacataact accttacttg caaaggctta acaatatcta tacaggtaca
   155941 acaaaaaact gtccaccaat tttctttggc ggacagtttt cacttaaaaa taaagaagca
   156001 actattcgta aattatgcta aaatagcagt agaacgcgtc ctgagggaat cgaacccccg
   156061 tctcaagaac cggaatctta cgtgatatcc actacactaa ggacgcaagc actgggataa
   156121 ttgtaactaa acagataaaa aattacaagg ggattggaca aaaaaatgaa atttgagcaa
   156181 aatttttcac aaaaacagca gcagacacag aaactagcca tgacgcaaca gttgcaacag
   156241 tccatccaag ttttacaata taacagtgag gagctgttgg cattcgttga gaatcaagca
   156301 atggaaaatc cattggtgga agttgttgag ccggaatggc agccagatta cataaaagct
   156361 tcttcatctt cttatgaagg agaagagaca aattacctaa atcagattcc tgatacaaaa
   156421 gggtctttat ttgattcatt gatcgaacaa gtccatttga attaccgaga tacttttttg
   156481 aggaaaatcg ttctttatct tgtagaatac atcgatttga acggattttt gacgattagt
   156541 ttagaagaag ccggaaaaga gattggagct acgcctatcc aaatgctcga tgcgttgact
   156601 ctgattcagc aattagaacc agctggagta ggtgcacgta gtttgcaaga atgtctcatg
   156661 cttcaaacag aacgagatga ttatgcgccg gaattagctt atatcgtgtt ggaagaatgt
   156721 tttgaggaac tggtggagcg aaaatggaaa gaaatcgcac aacgtttttc tgttgatcta
   156781 catgctgttc agcagatatt cgactattta caaacgttga gtccaagccc tggccggatt
   156841 ttcgaccgta gcagcgaatt gtacattatt cctgatgtcc gggtattagt ggacgacgat
   156901 aaaaatgtac aagttatctc aaaccgaaaa aatcagccaa atatacgctt tcaagagagt
   156961 tacttcaaac aaatgtcaca acaagcagat aaagaaactg aaaattattt aaaagaacgg
   157021 aaacaggaat tcgaatggtt gaagaaaacg atcctacaac gtggggatac aattttgcgg
   157081 gttgctcaag tcattgtttc ccggcaaaaa gctttcttta tcgacaagga acgtccaatc
   157141 aagcctctta cgttaaagga agttgcaact gagatagatg tgcatgaatc cacggtcagt
   157201 cgtgcagtca acgggaaata tttggaaaca gacttcggtg tatttgaatt aaaaaaattt
   157261 ttcactacac gaattcctac gaatagtaca gaacagacag aagacctttc agcagataca
   157321 gcgaagaaaa agctgcagga attagtcgat caagaagaca aaaacaagcc gttgtctgac
   157381 cagaaattgg ttgaattgct gaaaaaagat gagatagcga tctcaagacg aacagtagca
   157441 aaatatcgtg atttattagg aatcccgagt tcatcaaaaa gaaaacgtta tgataactaa
   157501 tcagtcaaaa gactagaaaa agtatttcac tttttctagt ctttttagtt gaaaaacgag
   157561 agcaaaactg ttagacttac agtcgtgagg ttgagatttc cctttattga tgtgaaattc
   157621 gaatagttaa tttctttttt tatcgtatat gggtcgtttt aagtctacat tggacgtaaa
   157681 gtgtccaact aaggagatcg ctatgctgga tgaaataaaa atgattgaat ctgttgcacc
   157741 agatattatc gaagtgttgc aagaacgcta taaaatttta cgaaatatct attggatgca
   157801 gccgattggg cgtcgaagtt tatcggaaag tatggggatc acagaacgag tcttacgaac
   157861 agaaacagac cttttgaaaa atctgaattt gatcgacacc tcaaaaagcg gtatgacgct
   157921 gacaactaag ggcgaagaag tttatcaaag ccttgaaaac ttcatggatc agcttttagg
   157981 tacgcatcaa accgaacaac agttggcaga atattttggt aaccaacgct gtatcgttgt
   158041 ttcaggaaac agcgaagagc agaccaaagt ggctgatgca ttcggcgaag cgctgagcga
   158101 agccttagac cgactacttc cagaaggcga gaatatcatc gctgtgatgg gaggaactac
   158161 tatggctgtg gtagccgaac aattgtctaa tctggaaaat aagaaacggc ataacctgtt
   158221 tgtccctgca agaggcggga ttggtgaagc gataactgta caggcaaatt ctgtcagtgc
   158281 aagaatggct gctaaagcaa acggtaacca ccgcgcactt tatgttccgg aacaattaag
   158341 tctggcaact tacaattctt tactcaatga gccatctatc caagaagtct taaacttgat
   158401 tagtgaagcg aattgtgtta ttcatagtat tgggcgtgca ttacacatgg cagcacgtcg
   158461 taagatgact gaaaaagaat tagtcatgct gaaacaagca aatgctgttg cggaatcttt
   158521 tggctacttt ttcaatgaaa aaggtgaagt cgtttataag gtccctagaa tcggcataga
   158581 attgcaagat ttggaaaaag tcccgatcat catggcaatc gcgggcggaa aatcaaaagc
   158641 taaagcaatt cgagcttata tgaaaaatgc accaaaacaa acgtggctca tcactgatga
   158701 agctgctgca aatgagattt taaaaggggt aaccctttaa aataaatatt tttttgattt
   158761 tcataaggag gaaatcttaa atgacagtta aagtaggtat taatggtttt ggacgtattg
   158821 gacgtctagc attccgtcgt atccaagatg tagatggaat cgaagtagta gcaatcaacg
   158881 acttgacaga tgctaaaatg ttggcacact tgttaaaata cgacacaact caaggacgtt
   158941 tcaacggaac agttgaagtt catgaaggtt cattcaacgt aaacggaaaa gaagttaaag
   159001 tattagctaa ccgcaaccca gaagaattac catggggcga actaggcgta gatatcgttc
   159061 tagaatgtac tggtttcttc acttctaaat cagcagctga aaaacattta acagctggtg
   159121 ctaaacgcgt tgttatctct gctcctggcg gaaacgatgt tccaacaatc gtttataaca
   159181 caaaccacga aacattaact ggtaaagaaa cagttatctc aggtgcttca tgtactacta
   159241 actgtttagc tcctatggct aaaacattga acgacaaatt tggtgttgtt gaaggattaa
   159301 tgacaactat ccacgcttac acaggtgacc aaatgactct agacggacct catcctaaag
   159361 gtgacttccg ccgtgcacgc gctgctgctg caaacatcgt tcctaactca acaggtgctg
   159421 ctaaagctat cggtttagta attcccgaat tgaacggtaa attagacgga gctgctcaac
   159481 gtgttcctgt accaacaggt tcattaacag aattagtaac agttcttgaa aaagaagtaa
   159541 ctgttgacga aatcaatgca gcaatgaaag aagcttcaaa cgaatcttac ggatacaaca
   159601 cagacgaaat cgtttcttca gatatcgttg gtatgactta cggttcatta tttgatgcta
   159661 cacaaactaa agtaatgaca gttggcgaca aacaattagt taaaactgtt gcttggtacg
   159721 acaacgaaat gtcatacact gcacaattag tacgtacttt agaatacttc gctaacttat
   159781 aagatagaca aacgtacaag tgtaaattat agacatgtac atttaaaagc ggggaagcaa
   159841 tcgcgcttct tccgcttttt ttgataataa gtttatt
//
LOCUS       DACDRN010000002       127594 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDRN010000002 DACDRN010000000
VERSION     DACDRN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 127594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 127594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..127594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.002"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..107)
                     /locus_tag="IWJ80_000158"
     rRNA            complement(<1..107)
                     /locus_tag="IWJ80_000158"
                     /product="16S ribosomal RNA"
     gene            26..169
                     /locus_tag="IWJ80_000159"
     CDS             26..169
                     /locus_tag="IWJ80_000159"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9459929.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            847..1536
                     /locus_tag="IWJ80_000160"
     CDS             847..1536
                     /locus_tag="IWJ80_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9459930.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1523..2731
                     /locus_tag="IWJ80_000161"
     CDS             1523..2731
                     /locus_tag="IWJ80_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9459931.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            3025..4296
                     /gene="serS"
                     /locus_tag="IWJ80_000162"
     CDS             3025..4296
                     /gene="serS"
                     /locus_tag="IWJ80_000162"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP9459932.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4845..6329)
                     /gene="guaB"
                     /locus_tag="IWJ80_000163"
     CDS             complement(4845..6329)
                     /gene="guaB"
                     /locus_tag="IWJ80_000163"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP9459933.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6834..7529)
                     /locus_tag="IWJ80_000164"
     CDS             complement(6834..7529)
                     /locus_tag="IWJ80_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP9459934.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7619..8719)
                     /gene="ychF"
                     /locus_tag="IWJ80_000165"
     CDS             complement(7619..8719)
                     /gene="ychF"
                     /locus_tag="IWJ80_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP9459935.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8728..8919)
                     /locus_tag="IWJ80_000166"
     CDS             complement(8728..8919)
                     /locus_tag="IWJ80_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP9459936.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8940..9830)
                     /locus_tag="IWJ80_000167"
     CDS             complement(8940..9830)
                     /locus_tag="IWJ80_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP9459937.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9817..10584)
                     /locus_tag="IWJ80_000168"
     CDS             complement(9817..10584)
                     /locus_tag="IWJ80_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9459938.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10596..11309)
                     /gene="rsmG"
                     /locus_tag="IWJ80_000169"
     CDS             complement(10596..11309)
                     /gene="rsmG"
                     /locus_tag="IWJ80_000169"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP9459939.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11541..13442)
                     /gene="mnmG"
                     /locus_tag="IWJ80_000170"
     CDS             complement(11541..13442)
                     /gene="mnmG"
                     /locus_tag="IWJ80_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP9459940.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13459..14856)
                     /gene="mnmE"
                     /locus_tag="IWJ80_000171"
     CDS             complement(13459..14856)
                     /gene="mnmE"
                     /locus_tag="IWJ80_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP9459941.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            15200..15538
                     /locus_tag="IWJ80_000172"
     CDS             15200..15538
                     /locus_tag="IWJ80_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9459942.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15706..16464)
                     /locus_tag="IWJ80_000173"
     CDS             complement(15706..16464)
                     /locus_tag="IWJ80_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9459943.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16482..17300)
                     /locus_tag="IWJ80_000174"
     CDS             complement(16482..17300)
                     /locus_tag="IWJ80_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9459944.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17297..17653)
                     /gene="rnpA"
                     /locus_tag="IWJ80_000175"
     CDS             complement(17297..17653)
                     /gene="rnpA"
                     /locus_tag="IWJ80_000175"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP9459945.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17771..17905)
                     /gene="rpmH"
                     /locus_tag="IWJ80_000176"
     CDS             complement(17771..17905)
                     /gene="rpmH"
                     /locus_tag="IWJ80_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP9459946.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18428..19762
                     /gene="dnaA"
                     /locus_tag="IWJ80_000177"
     CDS             18428..19762
                     /gene="dnaA"
                     /locus_tag="IWJ80_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP9459947.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19960..21090
                     /gene="dnaN"
                     /locus_tag="IWJ80_000178"
     CDS             19960..21090
                     /gene="dnaN"
                     /locus_tag="IWJ80_000178"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP9459948.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21319..21561
                     /gene="yaaA"
                     /locus_tag="IWJ80_000179"
     CDS             21319..21561
                     /gene="yaaA"
                     /locus_tag="IWJ80_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP9459949.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21548..22672
                     /gene="recF"
                     /locus_tag="IWJ80_000180"
     CDS             21548..22672
                     /gene="recF"
                     /locus_tag="IWJ80_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP9459950.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22669..24615
                     /gene="gyrB"
                     /locus_tag="IWJ80_000181"
     CDS             22669..24615
                     /gene="gyrB"
                     /locus_tag="IWJ80_000181"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP9459951.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24635..27106
                     /gene="gyrA"
                     /locus_tag="IWJ80_000182"
     CDS             24635..27106
                     /gene="gyrA"
                     /locus_tag="IWJ80_000182"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP9459952.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27342..27641
                     /locus_tag="IWJ80_000183"
     CDS             27342..27641
                     /locus_tag="IWJ80_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP9459953.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27691..28203
                     /gene="ssb"
                     /locus_tag="IWJ80_000184"
     CDS             27691..28203
                     /gene="ssb"
                     /locus_tag="IWJ80_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9459954.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            28229..28465
                     /gene="rpsR"
                     /locus_tag="IWJ80_000185"
     CDS             28229..28465
                     /gene="rpsR"
                     /locus_tag="IWJ80_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP9459955.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28608..30581
                     /locus_tag="IWJ80_000186"
     CDS             28608..30581
                     /locus_tag="IWJ80_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP9459956.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30587..31039
                     /gene="rplI"
                     /locus_tag="IWJ80_000187"
     CDS             30587..31039
                     /gene="rplI"
                     /locus_tag="IWJ80_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP9459957.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31313..32680
                     /gene="dnaB"
                     /locus_tag="IWJ80_000188"
     CDS             31313..32680
                     /gene="dnaB"
                     /locus_tag="IWJ80_000188"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP9459958.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33460..34752
                     /locus_tag="IWJ80_000189"
     CDS             33460..34752
                     /locus_tag="IWJ80_000189"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP9459959.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35539..36402
                     /locus_tag="IWJ80_000190"
     CDS             35539..36402
                     /locus_tag="IWJ80_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9459960.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36531..38153)
                     /locus_tag="IWJ80_000191"
     CDS             complement(36531..38153)
                     /locus_tag="IWJ80_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9459961.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38402..38923
                     /locus_tag="IWJ80_000192"
     CDS             38402..38923
                     /locus_tag="IWJ80_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9459962.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGTIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(39109..40839)
                     /gene="spxB"
                     /locus_tag="IWJ80_000193"
     CDS             complement(39109..40839)
                     /gene="spxB"
                     /locus_tag="IWJ80_000193"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP9459963.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            41004..41822
                     /locus_tag="IWJ80_000194"
     CDS             41004..41822
                     /locus_tag="IWJ80_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP9459964.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41924..42121)
                     /locus_tag="IWJ80_000195"
     CDS             complement(41924..42121)
                     /locus_tag="IWJ80_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9459965.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            42290..42868
                     /locus_tag="IWJ80_000196"
     CDS             42290..42868
                     /locus_tag="IWJ80_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9459966.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(43913..44416)
                     /locus_tag="IWJ80_000197"
     CDS             complement(43913..44416)
                     /locus_tag="IWJ80_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9459967.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44775..45800
                     /locus_tag="IWJ80_000198"
     CDS             44775..45800
                     /locus_tag="IWJ80_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9459968.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            46123..47001
                     /locus_tag="IWJ80_000199"
     CDS             46123..47001
                     /locus_tag="IWJ80_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9459969.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47477..47593
                     /locus_tag="IWJ80_000200"
     CDS             47477..47593
                     /locus_tag="IWJ80_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9459970.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48521..48859
                     /locus_tag="IWJ80_000201"
     CDS             48521..48859
                     /locus_tag="IWJ80_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9459971.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48953..49183
                     /locus_tag="IWJ80_000202"
     CDS             48953..49183
                     /locus_tag="IWJ80_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9459972.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49583..49954
                     /locus_tag="IWJ80_000203"
     CDS             49583..49954
                     /locus_tag="IWJ80_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9459973.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(50228..50920)
                     /locus_tag="IWJ80_000204"
     CDS             complement(50228..50920)
                     /locus_tag="IWJ80_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9459974.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50923..51708)
                     /locus_tag="IWJ80_000205"
     CDS             complement(50923..51708)
                     /locus_tag="IWJ80_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9459975.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51945..53345
                     /gene="lacG"
                     /locus_tag="IWJ80_000206"
     CDS             51945..53345
                     /gene="lacG"
                     /locus_tag="IWJ80_000206"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9459976.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            53370..53687
                     /locus_tag="IWJ80_000207"
     CDS             53370..53687
                     /locus_tag="IWJ80_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9459977.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53700..55373
                     /locus_tag="IWJ80_000208"
     CDS             53700..55373
                     /locus_tag="IWJ80_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9459978.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            55387..57018
                     /locus_tag="IWJ80_000209"
     CDS             55387..57018
                     /locus_tag="IWJ80_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP9459979.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(57509..59074)
                     /gene="guaA"
                     /locus_tag="IWJ80_000210"
     CDS             complement(57509..59074)
                     /gene="guaA"
                     /locus_tag="IWJ80_000210"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9459980.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(59133..60056)
                     /locus_tag="IWJ80_000211"
     CDS             complement(59133..60056)
                     /locus_tag="IWJ80_000211"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP9459981.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            60252..60857
                     /locus_tag="IWJ80_000212"
     CDS             60252..60857
                     /locus_tag="IWJ80_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9459982.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            61165..62181
                     /locus_tag="IWJ80_000213"
     CDS             61165..62181
                     /locus_tag="IWJ80_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9459983.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            62322..63623
                     /locus_tag="IWJ80_000214"
     CDS             62322..63623
                     /locus_tag="IWJ80_000214"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP9459984.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            63669..64331
                     /gene="deoC"
                     /locus_tag="IWJ80_000215"
     CDS             63669..64331
                     /gene="deoC"
                     /locus_tag="IWJ80_000215"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP9459985.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            64352..64750
                     /locus_tag="IWJ80_000216"
     CDS             64352..64750
                     /locus_tag="IWJ80_000216"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP9459986.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            64816..65898
                     /locus_tag="IWJ80_000217"
     CDS             64816..65898
                     /locus_tag="IWJ80_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9459987.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            66110..67672
                     /locus_tag="IWJ80_000218"
     CDS             66110..67672
                     /locus_tag="IWJ80_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9459988.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            67665..68798
                     /locus_tag="IWJ80_000219"
     CDS             67665..68798
                     /locus_tag="IWJ80_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9459989.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            68798..69754
                     /locus_tag="IWJ80_000220"
     CDS             68798..69754
                     /locus_tag="IWJ80_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9459990.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            69853..71019
                     /gene="deoB"
                     /locus_tag="IWJ80_000221"
     CDS             69853..71019
                     /gene="deoB"
                     /locus_tag="IWJ80_000221"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP9459991.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            71043..71861
                     /locus_tag="IWJ80_000222"
     CDS             71043..71861
                     /locus_tag="IWJ80_000222"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9459992.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            71878..72585
                     /gene="deoD"
                     /locus_tag="IWJ80_000223"
     CDS             71878..72585
                     /gene="deoD"
                     /locus_tag="IWJ80_000223"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9459993.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(72883..73569)
                     /gene="gpmA"
                     /locus_tag="IWJ80_000224"
     CDS             complement(72883..73569)
                     /gene="gpmA"
                     /locus_tag="IWJ80_000224"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9459994.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(73741..74322)
                     /locus_tag="IWJ80_000225"
     CDS             complement(73741..74322)
                     /locus_tag="IWJ80_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9459995.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(74455..75138)
                     /gene="rpiA"
                     /locus_tag="IWJ80_000226"
     CDS             complement(74455..75138)
                     /gene="rpiA"
                     /locus_tag="IWJ80_000226"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP9459996.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            75457..75870
                     /gene="rpsL"
                     /locus_tag="IWJ80_000227"
     CDS             75457..75870
                     /gene="rpsL"
                     /locus_tag="IWJ80_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP9459997.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVRD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            75954..76424
                     /gene="rpsG"
                     /locus_tag="IWJ80_000228"
     CDS             75954..76424
                     /gene="rpsG"
                     /locus_tag="IWJ80_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP9459998.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            76518..78605
                     /gene="fusA"
                     /locus_tag="IWJ80_000229"
     CDS             76518..78605
                     /gene="fusA"
                     /locus_tag="IWJ80_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9459999.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            78777..79964
                     /gene="tuf"
                     /locus_tag="IWJ80_000230"
     CDS             78777..79964
                     /gene="tuf"
                     /locus_tag="IWJ80_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9460000.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            80371..80679
                     /gene="rpsJ"
                     /locus_tag="IWJ80_000231"
     CDS             80371..80679
                     /gene="rpsJ"
                     /locus_tag="IWJ80_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP9460001.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            80710..81339
                     /gene="rplC"
                     /locus_tag="IWJ80_000232"
     CDS             80710..81339
                     /gene="rplC"
                     /locus_tag="IWJ80_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP9460002.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            81367..81990
                     /gene="rplD"
                     /locus_tag="IWJ80_000233"
     CDS             81367..81990
                     /gene="rplD"
                     /locus_tag="IWJ80_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP9460003.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            81990..82280
                     /gene="rplW"
                     /locus_tag="IWJ80_000234"
     CDS             81990..82280
                     /gene="rplW"
                     /locus_tag="IWJ80_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP9460004.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            82319..83152
                     /gene="rplB"
                     /locus_tag="IWJ80_000235"
     CDS             82319..83152
                     /gene="rplB"
                     /locus_tag="IWJ80_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP9460005.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            83195..83473
                     /gene="rpsS"
                     /locus_tag="IWJ80_000236"
     CDS             83195..83473
                     /gene="rpsS"
                     /locus_tag="IWJ80_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP9460006.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            83496..83843
                     /gene="rplV"
                     /locus_tag="IWJ80_000237"
     CDS             83496..83843
                     /gene="rplV"
                     /locus_tag="IWJ80_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP9460007.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            83857..84513
                     /gene="rpsC"
                     /locus_tag="IWJ80_000238"
     CDS             83857..84513
                     /gene="rpsC"
                     /locus_tag="IWJ80_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP9460008.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            84516..84950
                     /gene="rplP"
                     /locus_tag="IWJ80_000239"
     CDS             84516..84950
                     /gene="rplP"
                     /locus_tag="IWJ80_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP9460009.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            84940..85128
                     /gene="rpmC"
                     /locus_tag="IWJ80_000240"
     CDS             84940..85128
                     /gene="rpmC"
                     /locus_tag="IWJ80_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP9460010.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            85153..85419
                     /gene="rpsQ"
                     /locus_tag="IWJ80_000241"
     CDS             85153..85419
                     /gene="rpsQ"
                     /locus_tag="IWJ80_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP9460011.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            85477..85845
                     /gene="rplN"
                     /locus_tag="IWJ80_000242"
     CDS             85477..85845
                     /gene="rplN"
                     /locus_tag="IWJ80_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP9460012.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            85882..86190
                     /gene="rplX"
                     /locus_tag="IWJ80_000243"
     CDS             85882..86190
                     /gene="rplX"
                     /locus_tag="IWJ80_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP9460013.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            86218..86757
                     /gene="rplE"
                     /locus_tag="IWJ80_000244"
     CDS             86218..86757
                     /gene="rplE"
                     /locus_tag="IWJ80_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP9460014.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            86776..86961
                     /locus_tag="IWJ80_000245"
     CDS             86776..86961
                     /locus_tag="IWJ80_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP9460015.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            86997..87395
                     /gene="rpsH"
                     /locus_tag="IWJ80_000246"
     CDS             86997..87395
                     /gene="rpsH"
                     /locus_tag="IWJ80_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP9460016.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            87427..87963
                     /gene="rplF"
                     /locus_tag="IWJ80_000247"
     CDS             87427..87963
                     /gene="rplF"
                     /locus_tag="IWJ80_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP9460017.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            88124..88480
                     /gene="rplR"
                     /locus_tag="IWJ80_000248"
     CDS             88124..88480
                     /gene="rplR"
                     /locus_tag="IWJ80_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP9460018.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            88501..89001
                     /gene="rpsE"
                     /locus_tag="IWJ80_000249"
     CDS             88501..89001
                     /gene="rpsE"
                     /locus_tag="IWJ80_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP9460019.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            89016..89195
                     /gene="rpmD"
                     /locus_tag="IWJ80_000250"
     CDS             89016..89195
                     /gene="rpmD"
                     /locus_tag="IWJ80_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP9460020.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            89239..89679
                     /gene="rplO"
                     /locus_tag="IWJ80_000251"
     CDS             89239..89679
                     /gene="rplO"
                     /locus_tag="IWJ80_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP9460021.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            89679..90974
                     /gene="secY"
                     /locus_tag="IWJ80_000252"
     CDS             89679..90974
                     /gene="secY"
                     /locus_tag="IWJ80_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP9460022.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            91036..91683
                     /locus_tag="IWJ80_000253"
     CDS             91036..91683
                     /locus_tag="IWJ80_000253"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP9460023.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            91874..92092
                     /gene="infA"
                     /locus_tag="IWJ80_000254"
     CDS             91874..92092
                     /gene="infA"
                     /locus_tag="IWJ80_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP9460024.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            92125..92241
                     /gene="rpmJ"
                     /locus_tag="IWJ80_000255"
     CDS             92125..92241
                     /gene="rpmJ"
                     /locus_tag="IWJ80_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP9460025.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            92259..92624
                     /gene="rpsM"
                     /locus_tag="IWJ80_000256"
     CDS             92259..92624
                     /gene="rpsM"
                     /locus_tag="IWJ80_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP9460026.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            92652..93041
                     /gene="rpsK"
                     /locus_tag="IWJ80_000257"
     CDS             92652..93041
                     /gene="rpsK"
                     /locus_tag="IWJ80_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP9460027.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            93121..94059
                     /locus_tag="IWJ80_000258"
     CDS             93121..94059
                     /locus_tag="IWJ80_000258"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP9460028.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            94087..94467
                     /gene="rplQ"
                     /locus_tag="IWJ80_000259"
     CDS             94087..94467
                     /gene="rplQ"
                     /locus_tag="IWJ80_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP9460029.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            94739..95578
                     /locus_tag="IWJ80_000260"
     CDS             94739..95578
                     /locus_tag="IWJ80_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9460030.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            95554..96423
                     /locus_tag="IWJ80_000261"
     CDS             95554..96423
                     /locus_tag="IWJ80_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9460031.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            96413..97210
                     /locus_tag="IWJ80_000262"
     CDS             96413..97210
                     /locus_tag="IWJ80_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9460032.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            97413..98159
                     /gene="truA"
                     /locus_tag="IWJ80_000263"
     CDS             97413..98159
                     /gene="truA"
                     /locus_tag="IWJ80_000263"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9460033.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(98614..101283)
                     /locus_tag="IWJ80_000264"
     CDS             complement(98614..101283)
                     /locus_tag="IWJ80_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9460034.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(101521..101709)
                     /locus_tag="IWJ80_000265"
     CDS             complement(101521..101709)
                     /locus_tag="IWJ80_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP9460035.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            101979..102341
                     /locus_tag="IWJ80_000266"
     CDS             101979..102341
                     /locus_tag="IWJ80_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9460036.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            102393..104075
                     /locus_tag="IWJ80_000267"
     CDS             102393..104075
                     /locus_tag="IWJ80_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP9460037.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            104192..106228
                     /gene="recG"
                     /locus_tag="IWJ80_000268"
     CDS             104192..106228
                     /gene="recG"
                     /locus_tag="IWJ80_000268"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP9460038.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            106277..107278
                     /gene="plsX"
                     /locus_tag="IWJ80_000269"
     CDS             106277..107278
                     /gene="plsX"
                     /locus_tag="IWJ80_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP9460039.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            107400..107642
                     /gene="acpP"
                     /locus_tag="IWJ80_000270"
     CDS             107400..107642
                     /gene="acpP"
                     /locus_tag="IWJ80_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9460040.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            107910..108914
                     /locus_tag="IWJ80_000271"
     CDS             107910..108914
                     /locus_tag="IWJ80_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9460041.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            108915..109859
                     /locus_tag="IWJ80_000272"
     CDS             108915..109859
                     /locus_tag="IWJ80_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9460042.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            109859..110821
                     /locus_tag="IWJ80_000273"
     CDS             109859..110821
                     /locus_tag="IWJ80_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9460043.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            110848..111762
                     /locus_tag="IWJ80_000274"
     CDS             110848..111762
                     /locus_tag="IWJ80_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9460044.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            111788..113569
                     /locus_tag="IWJ80_000275"
     CDS             111788..113569
                     /locus_tag="IWJ80_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9460045.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            113917..114603
                     /locus_tag="IWJ80_000276"
     CDS             113917..114603
                     /locus_tag="IWJ80_000276"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9460046.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            114623..118204
                     /gene="smc"
                     /locus_tag="IWJ80_000277"
     CDS             114623..118204
                     /gene="smc"
                     /locus_tag="IWJ80_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP9460047.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            118213..119022
                     /locus_tag="IWJ80_000278"
     CDS             118213..119022
                     /locus_tag="IWJ80_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9460048.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            119033..120031
                     /gene="ftsY"
                     /locus_tag="IWJ80_000279"
     CDS             119033..120031
                     /gene="ftsY"
                     /locus_tag="IWJ80_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP9460049.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            120218..122485
                     /gene="gshAB"
                     /locus_tag="IWJ80_000280"
     CDS             120218..122485
                     /gene="gshAB"
                     /locus_tag="IWJ80_000280"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP9460050.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(122574..123026)
                     /locus_tag="IWJ80_000281"
     CDS             complement(122574..123026)
                     /locus_tag="IWJ80_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP9460051.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            123176..124126
                     /locus_tag="IWJ80_000282"
     CDS             123176..124126
                     /locus_tag="IWJ80_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9460052.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            124119..125078
                     /locus_tag="IWJ80_000283"
     CDS             124119..125078
                     /locus_tag="IWJ80_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9460053.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            125075..125830
                     /locus_tag="IWJ80_000284"
     CDS             125075..125830
                     /locus_tag="IWJ80_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9460054.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            125853..126806
                     /locus_tag="IWJ80_000285"
     CDS             125853..126806
                     /locus_tag="IWJ80_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9460055.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
BASE COUNT        40389 a        23379 c        26685 g        37141 t
ORIGIN      
        1 tctttttccg gtggagcaag ctccggtgga aaaagaagcg ttcgacttgc atgtattagg
       61 cacgccgcca gcgttcgtcc tgagccagga tcaaactctc ataaaaagtt cgaacaatct
      121 tgcgattgtt aagctcaatt tgtttgctag catattgctt gcttgttaaa aatgtttgtt
      181 gtcttgaatt gaatcaagac gccctacaca tttggtttgt cttactttgt tcagttttca
      241 aaggtctact tcatcaggag tgtttcccga ttcgtttttg gctgccgtag caacttttat
      301 atcatatcag agagacaagt aattgtcaac tcttttttaa aagttttttc gatttcgttt
      361 ttcgaaatct tccagcaact catttatcat aacttatcgt ttgttatttg tcaagaactt
      421 ttttgaagtt tttttcgata gaatcaagct tcgcaagtgg ttctttttta acgactcagt
      481 tatcttatca tgtcatttcc aacatgtcaa caacttattt tttattattt aataagttat
      541 tggtgttctt ttgaacaaat aataatatat caacatcaca tgtgaaagtc aataagaaaa
      601 tgcttattta tcaacatttc tagcttacat gatcattggt tagttctggt cctttttcgt
      661 tcgtctttat aactccgaga gacgtaaaat ccttttgttt tcagtttaat gttcatgttc
      721 ttgtcttatt cacaccaact agatgtctca ccttctaaaa ttttccttaa caaagtgctg
      781 cttttatcta tttctgcttt tttttgctag aataatagta acaatatgac tctggaggat
      841 ttatttatga aaattttgat tgctgacgat gataaagaaa tcgttgaatt attaagtatc
      901 tatgttcaca atgaggggta tgaagcagta aaggcttatg atgggaaaga agcattatct
      961 aaaatcagaa caatccctga tattgaatta cttattttag atatcatgat gcctgagatg
     1021 gatgggatgc aagtagtgaa agagctacga aaagaatccc aaataccaat cattatgctg
     1081 acagcaaaaa ctactgatat ggataagatc aaaggactgg tcgctggtgc agatgattat
     1141 gtgaccaagc catttaatcc gttagaagta atggctagag taaagtcttt attacggcgt
     1201 acaaaaatgc agattgcccc agatcagcct gatgtattag aagtaaatgc attaatcatc
     1261 aataaagact ctcatgaagt taaaacaatc gatgggaaag aaatccagtt aacagctttg
     1321 gaattcggta tcctctactt gcttgctagt catcctaatc gagtatttag cgcggatgaa
     1381 atttttgagc gtgtttggaa acaagaaagt attgtctctg ctaaaactgt catggttcac
     1441 gttagtcatt taagagataa gattgaagaa gcaactggtg gtgaaaaagt cattcaaacc
     1501 gtttggggag tgggctataa aattgacgca agatagtaaa cgagcaactg aaaaacgccg
     1561 tcggatcaat ttgacttcaa aagaaatcag cgaattgtta gcggaaggaa tcatcacgat
     1621 tatccttttg cttctattaa atgtttctat tctggttgtt atcagttcgg tgatcaacag
     1681 ttcaccttct ttgacgaatg cgatttggga ttcaaagaat atttttgcag aacgattgaa
     1741 cactgatttg ttctggaacg gccggaattt tattattccc tttttctttc tattagatat
     1801 tggtgttttg tattggcgct tgattcgaag atatcgccaa atgcaattgc ggcatatcat
     1861 cagcgaactt cactacatcg caaacggaaa ttatgatcat cgtatccctt ttgaactgag
     1921 tggtgactta agccgagtgg taacaagtat caatggattg gtggatagta ccgtagcagc
     1981 gattgaagac gaacgtaaaa tcgaaaaatc aaaagatgaa ttgatcacga atgtcagtca
     2041 tgatatccgt accccactca cgtccatcat tggataccta ggactgatag aagatggcca
     2101 gtatcactca gaagaagatc ttttaaagta tacgcatact gcttatatca aagcaaagca
     2161 gatgaaatct ttagtggacg acttatttga atacactaag gtacgccagc ctgctgtccc
     2221 ggtgaatttt tctgcctttg acatgatcca gttgatcgaa cagttagcag ctgattttga
     2281 gttggaagct tctaaaaaaa atatccaaat acttgttcaa tcaaaagtag actcccttat
     2341 catggatggc gataccgaaa aacttgtacg tgtcttcaat aatcttttga cgaatgcgtt
     2401 gaaatacgga aaaggagcaa cgaagatcgt aatcgaagta gaacgtatcg gttcagaagt
     2461 tgttgcaaca gtcaaaaata acggagcaat gatcccacaa caggcaatcg ataatctatt
     2521 cgaccgtttt tatcgtgtag aagaatctcg ttcacaagca actggaggaa ctggacttgg
     2581 tttagcaatc gctcaaagca ttgtggcttt gcacggcgga tacatttatg caaaatcaga
     2641 taagcaatgg acttctttca tcatgcacct tccaatcaag aaaaatgaaa aattacctat
     2701 tgatacacag gaagttattg acgaaagctg aaatattcgc tattcttatt acaattatta
     2761 gaataaatga aaaacacaga acccagtaca ctttattctt tttcagagag ctgatggcag
     2821 gtgtaaatca gcaaaggata atttggaagt tccagttcgt ttacgtgccc tttcgaaata
     2881 agaaaggtcg gttcaacagc cgttatgtgt tcaacaagtg cagctgacgc tgaacttggg
     2941 tggtaccgcg aatttcagac tgattcgtcc caagaataat gggatggatc agtctttttt
     3001 tattttcaaa aaggaggaaa tcatatgtta gatgtaaaaa tgatgcgaca aaatattgaa
     3061 gaagtacaag aaaaattgat gactcggggc gtaaaaaaag aaacattagc tgaattcatg
     3121 gaactagatg aaaatcgtcg taagctacta gtcaaaagtg aagaattaaa aaaatatcga
     3181 aatgatgttt ctgctgagat cgcccaattg aagcgtgcaa aagaagatgc ttctgctaaa
     3241 atcgctgaaa tgaaagaagt cggtggcaac atcaaagcat tggatactga aattgctgaa
     3301 gtggatgaaa aattgaaaca aattgcaact actttgccaa accttcccca tgattctgtg
     3361 ccagttggaa cagatgaaga ggacaatgta gaaatccgtc gttggagcga accacgtaca
     3421 tttgcttttg aaccaaaacc acactgggaa attgcagaaa atcttgatat tttagatttt
     3481 gaacgtggcg caaaagtagc cggtagccgt tttgtctatt acaaaggatt aggtgctcga
     3541 ttagaacgtg ctgtctacaa cttcatgctt gatcagcata tttacgaaca cggctatact
     3601 gaaatgatca ctccttatat cgtcaacagt aaagcgatgt ttggtacggg acaatttcca
     3661 aaattcaaag aagatgtttt ccaattggag aatactgatc taacattaat cccaacagct
     3721 gaagtaccat taactaacta ttataatgat gaaattttag atggtgcaga tttacctatt
     3781 tatttcactg cactaagccc tgcttttcgt tctgaagccg gaagtgctgg tcgggataca
     3841 cgaggactga ttcgtcttca ccaattcaat aaagtagaaa tggtcaaatt ttcggatgta
     3901 gagcattctt acgaagaatt ggaaaaaatg acagctgatg cagaagatat cttgaaaaaa
     3961 ttgcatttgc cttatcgtgt gatggcctta tcaactggag acatgggctt ttctgcagct
     4021 aaaacttatg acttagaagt atggatacct gcacaagata cttatcgtga aatcagttct
     4081 tgttctaact gtgaagattt ccaagcacgc cgcgcaatga tccgttatcg cgataatgat
     4141 ggaaaaatcc actatactca tactctgaat ggttcaggac ttgcagttgg ccgtacagta
     4201 gctgccattt tggaaaacta ccaaaatgaa gatggttctg tcacagttcc agaagcgctc
     4261 gttccttata tggggaatct gaaagttatt aaataaagag gctgtgaaaa agccgcttag
     4321 tttcgagtat taagaaaaat tttgtgaaaa tggctttcca catttttgca taaattggac
     4381 ttaataccga gaaactggct tttgaacacc atttatcgag aggttgtgaa atcagcgaat
     4441 taacctgagt attagagaaa aaaatggaaa aatagtccct catattttac cgtttgttga
     4501 actaataccg agggtcagct gattgaacac cgtttagtgg agaggtcgta agcctacccc
     4561 ccagctccta gttacaagga caaattcaaa aaacagctct tcatatttta tgaatgttgt
     4621 acttgtgatt cggaggtggc aagggaacac tgtttattca aaaagaggct gggacagaag
     4681 cgtttaactc caagaaataa gaagaaattc acgaaaattg cttttcaaat ttttgtgaat
     4741 ttcttcttat ttccgaagga gttgcttcta cttccgccgt ttatacgtgt ttagagcgtg
     4801 agacaaaagt gttctttact tttgtcccac gctctttaat gtttttaaga ttcaactgaa
     4861 tagtttggtg cttcttttgt gatttgtaca tcatgagggt gtgattcttt taatccactg
     4921 ccgctcattt ggataaattg cgcttcatca cgtaattgct gtagattggc tgccccaaca
     4981 taacccattc ctgaacgcaa accgccgatc atttggaaaa taatatctgc aacacttcct
     5041 ttataagcaa cccgaccttc gattccttct ggtactaatt tatttgcttc attgacacta
     5101 ccttggaagt aacggtcttt tgaacctttt tccatcgcgc ctaatgagcc catacctcgg
     5161 tatgttttga atcgacgtcc ttggaagatt tcgaattcac ctggtgattc atctgtccct
     5221 gccaacatac ttcctagcat cactgcatgt ccgccggcag ctaatgcttt gacgatgtca
     5281 ccagaatatt tgatccctcc gtctgcaata atcgctttgc catattgacg agcaactgat
     5341 gctgcatcat aaatagctgt gagctgaggt acacctacac cggctaccac acgtgtcgta
     5401 caaatcgatc ctggtccgat accaactttt acgacatcta cacctgcatc atataaagct
     5461 tttgtcgctt cagccgtcgc aacgtttcct gcaatcaatg ttgcatctgc aaaagttgaa
     5521 cggatttctt gaatttttcg gatcacacct gcactatgtc catgagctgt atcgatgatg
     5581 attgcatctg cccctgcttc taataaagca ttcgcacgct caaatgtatc gcttgttaca
     5641 ccaacggctg ctgctacaag caatcgacca tgttcatctt ttgctgcatt tggaaattcg
     5701 atcactttct cgatgtcttt gatcgtgatc aatccgctta aacgaccttc gttatctacg
     5761 attggtaatt tttcgatttt atgtttttgc aaaatctttt ctgcatcttt tagagaggta
     5821 cctactgggg cagtcactaa gttgtctttt gtcatcacat cagcaatcgg catgtgataa
     5881 tctgttacaa aacgcatgtc gcggtttgta attatcccta ccaattttct gttttcaagt
     5941 gtttcaacga ttggcacacc actgatacga tatttgctca tcaattcttc tgcgtctgct
     6001 acaagatgct gtggcgttaa gaagaacgga tcaatgatta cgccactttc agaacgtttg
     6061 acttttctta cttcatccgc ttgctggctg atcgtcatgt ttttatggat cacacctagt
     6121 ccgccttgtc ttgccatagc aatggccatc ttgctgtcgg taacagtatc catacttgca
     6181 ctgatgattg gaatattcaa cttaatattt ttagctaatt gtacgctcat atccacttcg
     6241 tttggtaaca cgtgactttc tgctggtatc aataagacat catcaaaagt aaatcctttt
     6301 ttcgtgaact ttgtttccca gttggacatt tttagtacca ctcctctaat ttagtttatc
     6361 tattaaaata attgattttt tcatttaagt caatgatcta cttggaatca agttagcttt
     6421 ctcatagaat accaatataa agcgtttaca tctgtgtgta ttaaaagaga ttgtaaaaaa
     6481 gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac agcttctcat gtttttgaat
     6541 tggttgggtt tattaccgca gatgcaaact tttgaacacc gtttagtgga gaggctgtga
     6601 aaaagctgct tagtttcgag tagtaagaaa aattttgtga aaatggcttt ccacattttt
     6661 gcataaattg gacttaatac cgagaaactg gcttttgaac accgtttatc gtaaaaaggg
     6721 actgtgacaa gacttttgtc acagtccctt actattattt aactatggat aaacaaaatg
     6781 aggttcttta ctacccctag atatctagca aaatagctga tctttttgct agtttatctt
     6841 cttgggacgg ataaacttcc aataatcccg tattttttct tcaagtagta acggattgct
     6901 aaagcagttg ccccgataat gataactact actggatcta atactggatt gataactggt
     6961 ggcaatagtg ttgtcgcaga aaacagtacg acccaaagaa gtgtcgttcc acctaggata
     7021 agcaatgtct tacccattcc tggtttcttg cttcgatctg ctccgggata ttcatattga
     7081 tagatgtatt tgtacatcat ataaaagacc cagccaccga ttgcagaacc tagaacgagt
     7141 gtagaaaagc cataagctgg cgttgttcca cgtgaaaaca atccggtaat cgcaatcatg
     7201 atacttaata gaccaagcaa cagcaacgta ttatctaacc acatcaataa aggggtggat
     7261 gaatttttta cttctgctgg cttattgatg attgcttccg ttctttcaga gactgtccca
     7321 aacaattgac gggctgttct tccgcctttt tgttctgcaa ctaaagtagg cagaatgtca
     7381 tgaagggctt ttactttttc ttcttcagac aaattcgctg cttctaaaga ttttttcaaa
     7441 tcaaaaatgt actgttgatt tcttttggtt aattttgttt ctaaactatt gttttctttt
     7501 acgattgctc gtaattgttc tggttccatt atctaatagt cctccttagc cgttcctttt
     7561 ctcaacggtc gtcacaaaag acattctacc atattctaca gctagtttga tagccctttt
     7621 acacattaaa gcggaataac attacgtcgc cgtcttgtac gacataatct tttccttcta
     7681 atcgaacacg tccagcttct tttgctgcat gcatgtttcc gtaatgatcc aagtcttcaa
     7741 aagagacagt ttctgcacgg ataaatccgc gttcgaaatc tgaatggatg attcctgctg
     7801 cttgtggtgc tttgattcct tttttgaatg tccaagcacg tacttcttgt tccccagcag
     7861 taaagtaagt agctaagcct aacaaatcat aagcagcacg gatcaactga tccaaacctg
     7921 attcttcgat tcctaaagct tctaaaaatt ccgctttgtc ttcttcatct aattctgcga
     7981 tttcttcttc tgcacgagca ctgaccacga tcacttctgc attttcttct gcagcaaagt
     8041 tgcgcacttc ttgtacataa tgattcgcgt ctgcatctgc tacgtcatct tctgagacat
     8101 tggctacata aagaattggt tttgtcgtca ataaaaatag tgatttaacg attttttgtt
     8161 cttcttcagt aaattcgatc gttcgggcag attttccttc ttccagtaca ggtttgattt
     8221 tatccagtac agctagttct gccactgcat ctttatcttt ggttttagca acttttgcta
     8281 cacgagtata gcgtttgttg atagattcta agtctgctaa cactagttcc aagttgatcg
     8341 tatcaatatc ttctagtgga tcaacacgtc cttctacgtg agtaatatta tcatcatcaa
     8401 aacagcgaac aacgtgacaa atagcgtcta cttggcggat atggcttaag aattgatttc
     8461 ccaaaccttc tcctttactt gcgcctttta cgattccagc aatatctgta aattcaaaag
     8521 tcgtcggaac agtttttttc ggatgtacaa gttctgttaa gcgttgcaaa cgccagtcag
     8581 gtacttctac catccctacg ttggggtcga tcgtcgcaaa gggatagttt gctgcttctg
     8641 ctcctgcttt tgtgatcgca ttaaacaaag ttgattttcc tacgttgggc aacccaacaa
     8701 ttccagcagt caatgccata aatttattca ctctttctct gcattttttt caattacttt
     8761 ttttaatttc ttttcaaaat ctcgacgggt catcatgacg atatgtccgc aattcataca
     8821 tctgattttg atatctgcgc ccattcgaat gacttcccag cgattcgtct ggcaagcgtg
     8881 aggttttttc atttcaacga tatcgtgcaa gtcgtacata aaaatagcct cctttttgtt
     8941 tattcttcat caaattgaat attcaagata tcaagaattc gagtcaaatc tttttgagaa
     9001 agatattcaa tctcgatctt acctttccca tttttttctt ggatagctac acttgtacca
     9061 aatttatcca tcaaacgatc ttcgctttct cggatataat atggtttttc ctttgctagc
     9121 cttgggattt ttttcttttc ttttccatca ttttgattta actcagaaac aagctgttcc
     9181 agttggcgga cagttaaatt ttcagttact gcacgattgg ctaatttcaa gatcaatgtt
     9241 ttatttttta aacctaacag cgtacgggct tgtcccatag ataaacgctg atcttgtacc
     9301 atttccttga caagatccgg taaagaaagc aagcgcaaat aattggcgat ataaggtctg
     9361 ctctttccta aacgactagc tacttctgct tgtgttagtt tcaggttttt catcagcatc
     9421 tcatatgctt ctgcttcttc taacggattc agatcttctc gctgcaagtt ttccagcact
     9481 gctacttgca tcatcgcttc ttcgtcaaag tctctaacga tagcagggat cgtttctttt
     9541 tcagctagtt tggacgctcg aaaacgtcgc tcccctgcaa taatctcata tccttttact
     9601 gcggaacggc gtacgatgat gggttgaaag acacctgatt gttcaataga attagctagt
     9661 tcctgcaaag atacttcttc aaatgtttta cggggctgat acggatttgg tcgcagctca
     9721 ttcaatggta gctggacaac ggtatcatct tttacatcca ccgtctctaa gttttctaaa
     9781 tcttgaaaca gtgcatcgat tcctcggcct aatcctttac ttttcttcac gacttaccac
     9841 ttcctttgcc aatgcttggt agacttcagc tcctcgagaa cgtggatcgt agtcgatgat
     9901 cggttttcca tgactaggtg cttctgataa acgaatattt cttggaatga tcgtttcgta
     9961 aactttttcg cggaagtatt tacgtacttc ttctacgact tcatttccca gatttgttcg
    10021 tgcatcatac attgtcagta agactccctc gatctctaat tctggattaa agtgtttttg
    10081 gactaaacga accgtattca gtaattggct taatccttcc aatgcgtaat actcacactg
    10141 aacaggtatc aaaatcgaat cacttgctgt aaatgaattg attgtcagat ggccaagaga
    10201 aggcgggcaa tcgatcaaaa tataatcata ctgactgctt acttctgcta aagatccttt
    10261 taatcttgat tctcttgcca tcattgacgt caattcgatc tctgctcctg ctaattggag
    10321 agtcgcaggc acaatactca aattttcgtg ttcagtgatc aaagttgcct catcaattgg
    10381 caattcattg actagaacat catagatgtc tcttgtcaca tcaggttttc tgatcccgac
    10441 accactggta gcatttcctt gtgcatccat atccactagc aatacttttt tgccaaggct
    10501 agcaagacaa gcacctagat tcaccgtcgt cgttgttttg ccgacgccac ctttttggtt
    10561 cgcaacagat attatttgtg ccatctcttc tcctcctatt tgattggctg cttattaggc
    10621 agtccgggct ttcttgggta tttctttggg gttggtttct ttttttgaat aacgatgata
    10681 tgccgttgat cagagacatt cggcaaggta accgcctctt ctttgaggaa tttaccacct
    10741 aataaagcaa tcgcttgttt tgcttctgtc aattcctctt cactttttgc tgctttcaat
    10801 gcataaaaat agccttcttg tttagccaag ggcaaacaca attccgctaa cacattcaaa
    10861 cgagcaaccg cacgagcagt gacaaggtca aatgatgcac gaaagtgagg attttgccca
    10921 aatgtttctg cccggtcatg atataaggaa gcatcgattc ctaataatcg ggttaactca
    10981 gataagaagg ttattcgttt attcagagaa tcaacgatcg ttatctgcag ttctggaaag
    11041 gcaattttta aaggtatgct aggaaaccct gcccccgaac caacatcgca taaagataaa
    11101 ggttgcgtaa agtctacttc aaatgctaat gtaagtgaat cgtaaaaatg cttcagatat
    11161 acgtcttttt catccgtaat agctgtaagg ttcatctttt cgttccattc gactaatgtc
    11221 cgataataaa tagcgaattg attcatttga tgatcggtaa gctctatccc tttttctgcc
    11281 aaggattggc gaaattcttc tggtatcata aatattcctt tcattgtgaa acagcaattg
    11341 tttcacagat tctctcccta ctttaacgca aaatctctag acatgcaatc cttccatatg
    11401 gattcttaaa aatttgaaaa gtaagaaaag ccgatagact ttttctttct agtatgactc
    11461 ataaaatata cacagaagag agaactagct ttgagcaatt tctgcaaaaa ctagttctct
    11521 cttctattta ttattacgta ttacgctggt tcactgactt tcgcaatctt cccttgttcg
    11581 atatagacca ttaggatact taggtctgca gggtttacgc cactgattcg gctagcttgg
    11641 gcaattgttt ctggttggat tttctgcaat ttttgttttg cttctgttgc taatccgttg
    11701 attgctgcgt agtcgatgtt ttctggaatc tttttcgctt ccatgcgttt taatttttct
    11761 actttttcca tggctttttt gatgtagcct tcatatttga tttggatttc tacttgttcg
    11821 atttcttttc gtgtcaattc ttcattttct ggaataaacc gtagaagttc tgcataagta
    11881 atctcaggac gtcttaaaaa gtcgcttgct agtacaccat cttttaatgg tgccgcatta
    11941 atcgttgcta aaaagtcttg gacttcttct gttggtttga ttcgtacatt catcaaacgt
    12001 ttgatttctt gttcaactgc ttcttttttc ttcaaataat tcttgtattg atcttctttt
    12061 actaagccta cacggtgacc catttctgtt aatcgcaaat ctgcattatc atgacgtaaa
    12121 agcagacggt attcagcacg tgaagtcagc aaacgataag gttcgttcgt tccttttgtt
    12181 accaaatcat cgatcatgac accgatgtaa ccgtcacttc gtttgatcac taaaggttct
    12241 tttccctgaa ctttcaaagc tgcattgata ccggcaatca gcccttgtcc agccgcttct
    12301 tcataaccgc ttgtaccatt tgtttgtcca gctgtataaa gattttcaac gactttagtt
    12361 tctaatgtcg gacggagctg gtgtggctct actacgtcat attcgatagc atatcctgta
    12421 cgcatcattt ctactttttc taatccagca atagaatgaa gcatgttgac ttgaacatcc
    12481 tctggtaaag aagtagataa accttgaaca tagacttctt ccgtatttaa gccttctggt
    12541 tccaaaaata attgatgacg tggtttgtct gcaaaacgca cgatcttatc ttcgatggat
    12601 ggacaatagc gagcgcctac cccttcaacg atccctgtaa acattggggc acgatgtaag
    12661 ttgtctcgaa taatttggtg agtcgtctca cctgtatagg tcagccagca agattcctga
    12721 tctagtttat aggcactatc tggtgtgctg aagctaaaat gattcggctc tttgtcacca
    12781 ggctgctctt ctgtaacaga atagtcgatc gtacttgatt ttactcgagg cggtgttcct
    12841 gttttgaacc gttcgatagc aaagccaagt tcttttagat gattcgctag tccaatagaa
    12901 ggttgagagt tattcggacc tgatgaatat ttcaattcac cgataatgat ttctccgcga
    12961 agtgctgttc cggctgtaat aacaactgct ttggcccggt agctcgctcc tgtggacgtc
    13021 actactccat gacaaacacc atcttcgacg atcagttctt cgacgattcc ttgtcttagc
    13081 gtcaaatttg gttctttttc gatcgtatgt ttcatttctg ttgaataagc atgtttgtct
    13141 gcttgtgctc gcaatgctcg gacagctggg ccttttcctg tgttcaacat acgcatttgg
    13201 atataggttt tatcgatatt cttacccatt tctccgccta acgcatcgat ttctcgaacg
    13261 acaacgcctt tagctggtcc gcctacagaa ggattacatg gcatgaatgc taccatgtcc
    13321 aaatttattg tcagtaataa tgtttttgat cccatacgtg cagcagcaag tgcagcttct
    13381 gaaccggcgt gtcctgcgcc aacaacgatc acgtcatacg catctgcttc ataatgttcc
    13441 aatcgtttgt tcctcctttt attttcctaa acaaaattga ctgaataatt gagtgattag
    13501 ctcatcttgt acactgtcgc cgacaacttc tcctagatag tcccagcaac gcgtcatatc
    13561 tatttgaacg agatccacag gcattcctga atcaatacct gcaatgactt cttcaagtga
    13621 gagggaagct ttttctaaca acgcgatatg gcgggtatta gaaacataag tagcgtctct
    13681 ttctcctgtt tctccgctga agaataaatc agaaatagct gattccaaac gatctagtcc
    13741 gtcgttttta gctacggaaa ttgaaaaaac tggttcattt tcgatcaatt tcttcaattt
    13801 ttcttgttcc agctgtgctg gcaaatctgt tttatttaga agaatgatcc gtttcaatcc
    13861 acttgtcgct tccaataatt gttcatcttc tgctgtcagt ggttcgcttt ggtttagaac
    13921 aagtaagatc aagtctgctt ctgctaatgc ttttcggctt ctttcgaccc cgatccgttc
    13981 caccacatct tctgtttccc gaattccagc tgtatcgatt agcttgagcg gtaccccccg
    14041 aacattgaca tactcttcga ttacatctcg agtcgttcct gcaatatccg taacgattgc
    14101 tttttcttct cttaacaaat ggtttaacaa acttgatttg ccgacatttg gccggccgat
    14161 gattgccgta cttaaccctt cccgcagaac cttcccttgc ttggatgtag caagtagcgc
    14221 ttggattctt tgttggatca tctgcgcttt ttcaagcaat aacttcgtag tcaattcttc
    14281 gacatcatca tattctggat aatcaatatt tacttctact tgtgctaatg tctctaaaat
    14341 ctcttgtctc aatgagcgga tcaaagcaga cagattgccg tctaactgat taagagctag
    14401 tcccattgct ttgtctgttt ttgcccgaat caaatccatc acagcttctg cctgtgaaag
    14461 gtcaacacga ccattcaaga atgctcgttt tgtgaattcg cctggctcag ctaatcttgc
    14521 accttctctt aacaatagct gcaaaatttg attgactaca acgatcccac cgtgacaatt
    14581 gatttctact acatcttccc gcgtaaatgt ctttggtgct ctcatcacgg agaccatgac
    14641 ttcatcgaca agctgattgc tcttgggatc aacgatatgt ccataatgga tcgtatgact
    14701 tggtacttct gataatcgtt tgtttccaga ttgataaact ttatctgcta gttgcacagc
    14761 ttgatcgcca cttaaacgaa cgatactgat tgcaccttct ccaggtgggg tagaaattgc
    14821 cgcaatcgta tcaaattcta aagtaatttc agccattttt tcacttcctt tttcattttg
    14881 cgcacaaaaa aagtgcccac tccacccttt atgcacaata aacggtgaac ttcgcacttt
    14941 gactgcttgt tcttgattaa attctggtca tagattatca cacttgctat aaggaatcaa
    15001 gtgtttaaaa caattagatg aagttgagcc tgttctgctt ccattacctt atatttaatt
    15061 gtccttggat gtctctttgc gttctttggt ttattcgaag agaagatact gtataaacaa
    15121 ggctttatct ttcttaaaaa aaacggtaca ctaaatggta gcgctatcta aactatacac
    15181 ttttgaaagg agaaaatatg tgagcccgtt cagatctgct gcattggaag aagaaagtga
    15241 ccctcggatc aacgatgtcg ttttatctac agggaatatc aaccagaaat atatcgtccg
    15301 cgatatcgtt tttgttgtgg aatgcttaga agctgatttg tttgacccat cttttcccaa
    15361 agatgacctt ttcttaacaa cgaagaggaa attaaagacg aaggtacttg aatatggtgc
    15421 gaatgcagta atcaactgtc attttgagta tcagcaaaaa aaacgcgacg gaaagtattt
    15481 tctggaactt tttgcttatg gaacagtgat ccaatttatc caaacaacga ttggttaaat
    15541 gaaacagaca aaaaaagcgg atcaaaactg aagacagttt cgatccgctt ttttctatca
    15601 aagttagtga ctacttcatc gtatactctg ataagaatga tttctagtaa aatatacttg
    15661 gtttgaaaaa ctttgtcaac gtgatttatt caaaaagtaa tggaatcaat aatatttttt
    15721 agcaggttct actactaaat aacgataagg ttcctcacct tccgaatgtg tttttacatg
    15781 ttcatctttg cttaatgcag catggatttg tttacgttca aatgcaggca ttggttctaa
    15841 gaaaacaggc tgccctgtcc gttttacttt ttcagccgtg cgatcagcta aacgctgaat
    15901 gatctcttgg cgcttttcac ggtaattgcc tacattgact actacagaca gcttatttct
    15961 tgctaaacga tggatgaaga cttgtgccag atattgcaat gcattcagtg tttttccatg
    16021 tttcccaatc agtatgcctt gcttttgtgt ttctaaatgg aaaacaacca tattatttga
    16081 ttctctttcc attttgacaa gagctggagc atcaagctgt tttgaaattt cagtaagata
    16141 aactgccaat tccgttaaag ctgcttcatc acctagctca cttacttctt ctgttttttc
    16201 ttcaagaatc gtttcgttga ctacctcgat ctcagctgtt ggttcttctt ccaaaatttc
    16261 ttctacagtc tgttctactg tttcagaaac tttttcgctg atacttggct ggatcgaaag
    16321 ccgtgcattc tttcgaccca ttcctaggaa accttttttt ccttcttcca gtatttcgat
    16381 ttcgacttgg tctttggcca aacccaaatc agccagacct agttggacag cttcgtctat
    16441 tgttgcagct tcataaatcg gcattagcat tacctcccct attatttccg ttttttctta
    16501 ggattcattg ctttcttaag agcacgttct ctttcgcgga ttctccgtgc ttcttcttca
    16561 cgctcttttc gaattttgaa agggttgttg atcagcagcg tttgtacaac ttggaaggca
    16621 ttggaaacta cccagtataa tgaaagaccg ctggctaaat tgacacccat cactaaaatc
    16681 attacaggca tcacgaaatt catgatcttc attgatgcat tcgattcaat ttgactcatg
    16741 ctagataaat acgtacttgc aaaagtaaag attgctgcta aaatcggcaa aataaatgtt
    16801 ggatccgcgt tccctaagtt caaccataag aaatgccctt gtttcaactc tggtacacgt
    16861 gaaatagatt gccataacgc catcaagatt ggcatttgta ctaataaagg aagacaacca
    16921 gcatatggat tgacgccatt ttctgcatat aaacgctgct gctcttctct taacttactt
    16981 tgtgtttccg gatctcttga actatattgt tgctgcagtg cttttaattt cggttgtaat
    17041 tcttgcgttt tacgcatgct ctttgtttgg aaatgcatca acggcaatag gatcacgcgg
    17101 atgatcagtg taaataaaat gatccctatc cctgcattac caaatgacaa cgccttgatc
    17161 gcctcagcaa agtaataaac aatataacga tcccaaattc ctgtactctg tgcgctgact
    17221 tccccagttc cgcaaccgga taagacgatg accaaactaa cgataccggc catcaaaagt
    17281 aaacgtttgt atttcttcac ttactctttt cctctctgtc gattattttt gcaagtttta
    17341 agacgtggac taaattggag cgaacctcat cataagtcaa ccccttcaca cttggtctag
    17401 caatcacaat aaaatccaac tccggatcaa tttcttcttt tatagaataa actgctgctc
    17461 ggatttttcg tttgacttca ttgcggttta ccgcgttgcc gacctttttt cctacagata
    17521 aaccaactcg aaaatgaggc tgtcccggtt tttccaaacg gtaaacgacg aatttgcggt
    17581 tagcaaaaga atctcccgta tggaacacgg cttgaaactc tcgttctttt tttactcgat
    17641 acgcttttct cattagtttt ctttcctttt ctatctctca cttacgctca taatcatacc
    17701 ataattacga ttgtcctttc atcatttccc taaaaaagag tataaaaaaa accactgaaa
    17761 ccccagtggc ctaagctgaa agaacttttc ttcctttgcg acgacggcta gctaacacac
    17821 gacgtccatt tttagtactc atacgtttac ggaatccgtg aactttttga cgtttacgtt
    17881 tatttggttg gtatgttctt ttcatatatt ccacctccaa aatgttctta tctattttat
    17941 aaacacagac atactttgat agtataacga caaagtagtg attttgtcaa tataaaatag
    18001 aagatcgatt gttaaaactt atccacaaga ttattttttt ctatgggaat cctatgtgga
    18061 aaaagttatg cacagggatt atccacagat aataaaaggc tggttttaca cagaaaacac
    18121 acgtgtaaaa aaagtaatcc acaatcaggc gatttcctgt gtattccgtc tcctaactat
    18181 tggtatatct gtatttttta tccacagacg caagaggaaa tctattggtt tttacgagtt
    18241 ttccacctgt gcattagtgg tgtggaaaac tttttttgac aactgtgttt ttctcttttt
    18301 tctttttgtc gattgtgtgg ataattgtaa ttaaaaaatt tttttcttcg gtttctgtgg
    18361 aaaactattg aaaaaaatgc taaattagta ctagttattc actttttaat gaaggaggag
    18421 gccttatatg gtatccctcg atgctttatg gaatgaatta aaagcaacat accaaaagga
    18481 tttatcgcct gctagctata atacatggat cgaaacagcg aaccctcgaa cccttgatca
    18541 aaaccagctc gttgttgagg ttcctagcaa gattcataaa gaatattggg aaaaaaacct
    18601 ggcgacaaaa atcgttgaag tcggttatat gttatcagga aatgagatta tccctcgctt
    18661 tattactggc gaagaagcag agcaagaaga agttatagaa gaaaaaaatc caaaagttgt
    18721 ggcacctagt ccgctaaaaa aagccatgct gaaccctaaa tatacctttg atacgtttgt
    18781 cattggtaaa ggaaaccaga tggctcatgc agcagcgctc gttgtagcag aagatccagg
    18841 ttctatttat aacccgctct tcttttatgg aggcgtggga cttggtaaga ctcacttgat
    18901 gcatgcaatt ggtcatcaaa tgctgcaaag tcagcctaat gccaaagtaa aatatgtaag
    18961 cagtgaaact tttgctaatg atttcatcaa ttcgatccaa aacaaaacag cagaagagtt
    19021 ccgtcaagaa tatcgaaatg ttgatttact gctagtggat gacattcaat tttttgcaga
    19081 aaaagaagct acgcaagaag agtttttcca tactttcaat gccttgtaca atgaagggaa
    19141 acaaatcgta ctaaccagtg atcggttgcc aaacgagatt ccgaaactgc aagaacgcct
    19201 tgtttctcgc tttgcatggg ggctgtctgt tgatattaca ccgccggatc ttgaaacaag
    19261 aacagcgatt ttacgtaaaa aagcggcggc tgaacgtcta gagatcccag atgacacact
    19321 aagctacatc gcaggacaaa ttgattctaa cattcgtgaa ttagaaggtg cgcttgttcg
    19381 agtgcaagct tttgctgcga tgaatagtga agatatttcg actagtctag ctgcagacgc
    19441 actgaaaacg ttaaaatcag gaaaaggaca tcctcaattg tcaatcctgc agatccaaga
    19501 agaagtggca aagtattatc acatccaatt gaaagatctc aaaggaaaga aacgcgtcaa
    19561 atctattgtt gttcctagac aaatcgctat gtatttggca cgtgagctca cagataattc
    19621 tttaccgaaa atcggagctg aattcggtgg caaggatcat acaacggtta tccatgctca
    19681 tgaaaaaatc cagcagctga tggattctag tgtcagtatg caaaatgaag tatcagaaat
    19741 caaaaacttt ttactaaatt aatttgtcga ttctatcaac tgaaaatagc atgtggataa
    19801 aagaaaaaaa cgttcaaaag ttatacacac tttttccaca gctgtttttt cgtttctctc
    19861 ttacgttttt tgagttttcc acagaatcaa catgccctat tacttttatt attcttttta
    19921 actaataata aataaacaaa cattcaaagg agatacatca tgaaagttac tttaaaccga
    19981 gctagcttta tgcaggaatt gcaaactgtt caacgagcta tttcaagcaa aaccacgatc
    20041 cctattttga caggtgtaaa aatcacactg acacaagaag gtttgacttt gacggggagc
    20101 aacgctgata tatcaattga aacttttttg tctgttgaaa acgaaaaagc aaatatgcaa
    20161 atcgaatcta ctggttccat tgttttacaa gcacgtttct ttagcgaaat cattcggaga
    20221 cttcctgaag aaacatttac tttagaagtt ttagaaaata aacaagtagc gatcacttct
    20281 ggaaaagcga attttatcgt aaatggatta gatgcagata actatcctca tcttcctgtt
    20341 gtcgaaagcc ataaccagat gaaattacct gtacacgtat tgactaaact aatcaacgaa
    20401 acagtttttg ctgtctcaca acatgagagt cgtccaatct tgacaggtgt gcatttcatt
    20461 ttatctgata attctttatt agctgtagct actgattctc accgtctaag tcaacgcgtg
    20521 attccagtag aacaagcggc tgatcatttt gatattgtta ttcctggaaa aagtttgatc
    20581 gaattatctc gctcattaac aaatgaagaa gaaatcgtcg aaatcagcat tatggaaaac
    20641 caagtgttgt tcaaaacaga aacgatgtat ttctattccc gtttgctaga aggaaactat
    20701 ccagatacca atcgtttgat tccttcaagt tttaatacag aggttgaatt ttctgttcct
    20761 agctttttag cggcgatcga acgggcttct ttactttctc atgaaggccg taacaacatc
    20821 gttcgtttat ctattcgtcc agatgcggtt gttttatacg gaaattcgcc agaaatcggg
    20881 aaagtagaag aaagcttgag ttatacagca agcagtggcg atccattaga tatttctttc
    20941 aatccggatt atatgaaagc agcgttgcgt gcatttggag atatgagcat caaagtgaaa
    21001 tttatctctg ctattcgtcc atttacattg gaaccaacgg aagatggcgt tcaatttatt
    21061 cagctgatta ctcctgtacg tacaaattaa caatgatttt caatgttctt gaaaatttaa
    21121 ttgaaaagaa atagaaaaaa taaaatttta aacagaaggc cctaaaatct cttttaaggc
    21181 tttctgttta tttttgttta aatcaccttt tttagattaa atcgtcttat atggaaaaat
    21241 atatggataa atttgtttta ttgcgaaaaa aaaggtataa tagagtatac ttttaataat
    21301 gaaaatgagg gatgggcatt gaaacaacaa gtcattctaa atacggaatt catgacgttg
    21361 ggccaaatgt tgaaagaagt cacagttatc ggtagtggcg gacaagcaaa atggtatttg
    21421 gcagaaaaca ctgtgttagt cgatggcgaa ccagaaaatc gtcgtggacg caaactttat
    21481 cccggcatga tggttgaagt tccagaagta ggaacttttt ttatggtaaa aaaaggaaac
    21541 gacgataatg aggctgaata agctgtattt aaaaaattat cgaaattacg aagagctgaa
    21601 catcgaattt tccaagaacc tcgtcatctt tttaggagaa aatgcgcaag gaaaaacgaa
    21661 tcttttggaa agtatctacg tattagctat gacgcgaagt catcggacaa gtagtgagca
    21721 ggaattgatt ggctggactg acgatcaggc gatgattcaa ggagagatca caaaaggttc
    21781 atccactctt ccattagaaa tcttattatc taaaaaaggc cgaaaaacca agatcaacca
    21841 tatcgaacag aaaaagttaa gtacttacgt gggacaaatg aatgtcattt tatttgcacc
    21901 agaagatctt tctttagtaa aaggcagccc gcagatcagg cggaagtttt tagatatgga
    21961 attaggccag atcaatccaa tctatcttta tgatcttgtc caataccaag caattttgaa
    22021 acaaagaaac cagtacttga agcaattgaa tgaaaaaaaa caaactgatt tgctttattt
    22081 ggatattttg tccgaacaac tagcagcgtt tggcagcaaa gtattgaaag caagagcaca
    22141 gttcgtcaag cgattggagt tttgggctaa ttctttgcat cagcaaatca cccatcaaaa
    22201 agagcagctg gaaatcgagt atctcaccgc agtcgattct ttagaaacac atacgcaaga
    22261 gcaaatccaa gaacaatttc tcgcactttt gaaccaaaat aaaaagaaag acctttttcg
    22321 gggaacaacg acagttggtc ctcatcgtga cgatctttcc ttttttatta atcagaaaaa
    22381 tgtgcagact tatggatcgc aaggacagca aagaacaact gcactaagtg tcaaattagc
    22441 agaaatcgat ttgattaaag aagagacagg ggaatatcca atccttttgt tagatgatgt
    22501 gatgagcgag ctggatgata atcgtcaact ccatctgcta gaaacaatcg aaggaaaagt
    22561 tcaaacgttc ttaacaacaa cgacacttga ccatgtaaaa aataaaatga ctgttgaacc
    22621 tgagattttt tatgttcaac aagggaaact aaaaggagaa gaaactgaat gacagaagaa
    22681 agaagtttag tagaacgcgc taaagagtat gatgccagtc agattcaagt acttgaagga
    22741 cttgaagccg ttcgtaaacg acctggcatg tatatcggtt cgactagttc tgagggtctg
    22801 catcaccttg tttgggaaat tgtcgataat tcgatcgatg aagtattagc aggttttgca
    22861 acgaaaatcc atgtgattat tgaaaaagac aacagtatca ccgttattga cgacggtcgg
    22921 gggatacctg tagatatcca ggcaaaaact ggacgtcctg cagttgaaac ggtctttact
    22981 gttttacatg ctggaggaaa attcggcggt ggtgggtaca aagtttctgg tggacttcac
    23041 ggagtaggat cttctgttgt aaacgcgctt tctacacagc ttgatgtcaa agtatacaaa
    23101 gaaggaaatg tgtactatca agaatatcgt cgtggagctg tagtggacga cttaaagatt
    23161 atcgaacaaa cggatcgcca tggaacaacg gttcacttta cgccagaccc tgaaattttt
    23221 acagagacaa ctgagtttga tttttcaaaa ttggctaccc ggatccgtga acttgctttc
    23281 ttgaaccgcg ggatgcgtat atcgattgaa gacaaacgag aagaagaacc agtcatcaat
    23341 gaataccatt atgacggcgg gatcaaaagt tatgtcgaat acttgaatgc aaacaaaacc
    23401 gtgatttttc cagaacctgt atatttggaa ggtgaacagc aagatatcgc agttgaagtc
    23461 tctatgcaat atacagacgg ctaccattcc aatatcatga gttttgccaa caatatccat
    23521 acgtatgaag gtggaacaca tgaatcagga ttcaagactg ctttaactcg tgtgataaat
    23581 gattatgccc gaaaacaaaa gctgatgaaa gaaaatgacg acaacttgac cggtgaagac
    23641 gtgcgagaag gattgacagc tgttatttct atcaagcatc ctgaccctca attcgaagga
    23701 caaacaaaaa caaaattggg caattccgaa gtacggacag tcactgatcg gttatttagc
    23761 gaacatttca tgaaattctt gctggaaaac ccaagtgtgg gccggcaaat tgttgaaaaa
    23821 ggattactag cttccagagc tcgtctagca gcgaaacgtg cgcgtgaagt aacgagacgt
    23881 aaaggtgctt tagagattag taatctgcca ggaaaattag cggactgctc tagcaacgat
    23941 cctgaaaaat gcgaattgtt catcgtcgaa ggagattcgg ccggcggatc agctaagcaa
    24001 ggtcgtaatc gagagtttca agccatcttg cctattcgtg ggaaaatctt gaatgttgaa
    24061 aaagcaagca tggacaagat cttggcaaat gaagagatcc ggtctttatt tactgctatg
    24121 ggtacagggt ttggagaaga ttttgatgta tcgaaagctc gctaccacaa attagttatc
    24181 atgactgatg ccgatgtcga cggcgcgcat atccgtacat tgctgctgac tcttttttat
    24241 cgttacatgc gtcctattgt tgaagctgga tacgtctata ttgctcagcc acctttatac
    24301 ggggtaaaac aagggaagaa tattacgtat gtacaaccag gaaaaaatgc agaagaagag
    24361 ttgaaacaag tcgttgcttc acttcctgcc tcaccaaaac caagcgttca gcgttataaa
    24421 ggacttggag aaatggatga tcatcaacta tgggaaacaa ctatggaccc aagcaatcgg
    24481 atgatggccc gcgtcagtgt ggacgatgct attgcagcag atcagatatt tgaaatgttg
    24541 atgggggatc gagtagaacc ccgtcgtgca ttcatcgagg aaaatgcaca ctatgtgaag
    24601 aacttagata tttaatcatg aaggaggaaa aagaatgagt gaagaaatca gagaaaacat
    24661 ccatgatgtc aatctaacca gcgaaatgaa agactccttc atcgattatg cgatgagtgt
    24721 tattgtcgca cgtgcacttc ctgatgtcag agatggtctg aaacccgttc atcgccgtat
    24781 tttatacggc atgaacgaat taggtgtaac accggataaa gcccataaaa aatctgccag
    24841 aatcgtcgga gatgtcatgg gtaaatacca tcctcatgga gacattgcga tctatgagtc
    24901 aatggtacgg atggcacagc cattcagcta ccgttatatg ctagtggatg gtcatggaaa
    24961 ctttggttct gttgacggtg acggtgccgc cgctatgcgt tataccgaag cacggatgag
    25021 caaaatcgct acagaaatgc tgcgtgatat caataagaac actgttgatt tccaaagcaa
    25081 ctatgatgat acagaaaaag aaccagttgt actacctgct cgcttcccga atcttttagt
    25141 gaacggaaca acggggatcg ccgtaggtat ggccacgaat atcccacctc ataacttgtc
    25201 tgaggtagta gcggctatcg atttgctgat ggataatcct gatgtgacga ccaatgaatt
    25261 gatggaagta ttacctggac cagattttcc aacaggtggt ttggttatgg ggaaatcagg
    25321 aatccgaaga gcgtatgaaa caggtaaggg ttctattacc gtacgtgcaa aagttgagat
    25381 tactgagatg ccaaatggta aagaacggat catcgtgaca gaattgcctt atatggtcaa
    25441 taaagcaaaa ttgatcgaac ggatttctga actgcacaga gacaagcgta tcgaagggat
    25501 cacagatttg cgggacgaat cttcccgtga aggtatgcgg atcgtcattg acatccgtcg
    25561 tgacgccagc gcatccgtta tcttgaataa cttatataaa ttgacatcct tgcaaaattc
    25621 atttggattc aatatgttgg cgatcgaaaa aggtgtgccg aaagttttga gcttgaaaca
    25681 aatccttgaa aactatatcg aacatcaacg cgaagtcatc acacgacgga cggtctttga
    25741 aaaagaaaaa gcagaagcaa gagcacacat cttagaaggt ttgcgtattg ctttggatca
    25801 tatcgatgaa atcattgcga ttatccgcgg gtctaaatca gatgacgagg caaaagcaac
    25861 aatgatcgaa cggttcgatt tatccgatcg ccagtctcaa gctattttag atatgcgttt
    25921 acgccgattg accggtttgg aacgggaaaa aatcgaaaat gaataccaag aactcttaaa
    25981 actgattgaa gatttaactg acattctggc aagaccagaa cgtgttacag aaatcatcaa
    26041 gaatgaatta gcagacttga atcaacgatt tggtgataaa cgacgcacag aattactagt
    26101 cggtgaggtc ttaagtctgg aagatgaaga tttaatcgaa gaagaagaaa tcgttattac
    26161 attgacaaac aatggttaca tcaaacgttt ggcaaataat gaattcagag ctcaacgaag
    26221 gggtggacgt ggtgtccaag gaatgggtat ccacaatgac gacttcgtca aaaatctggt
    26281 ttcttgttct acccatgata cattgttatt ctttacgaat aacggaaaag tgtatcgggc
    26341 aaaaggatac gaaattccag aatacgggcg tacagccaaa ggaatcccaa tcatcaattt
    26401 actaggtatc gattcgagtg aaagcatcca ggcaatcatt gcggtgacgg gagaagcaga
    26461 agaaggccac tatctcttct ttacgacgcg tcaaggtaca gtaaaacgta cttctgtcac
    26521 tgcttttgct aacatccgaa gtaatggtct gatcgcaatt ggtttgaaag aagacgatga
    26581 gctcgtcaat gtgctactga cagatggtca atctaatatc attattggaa cacataacgg
    26641 ctactctgtc actttcaaag aagaagcagt ccgcgatatg ggacgtacag cttccggggt
    26701 cagagggata cgcctgcgtg aggaagatta tgtcgttgga gcagccttaa tgaaggaaaa
    26761 tcaagaagta ttagtgatca cagaaaaagg ttacggaaaa cggacaaaag tatcggaata
    26821 tccagtgaaa ggccgcggag gtaaagggat caaaacggcg aacatcactg aaaaaaatgg
    26881 tccactagct ggattgacca ctgtttctgg agatgaagac atcatgctga ttacagataa
    26941 aggtgtcatt atccgtttca acgtatcgac tgtttctcaa actggacgtt caacattagg
    27001 cgtacgtttg atgaagatgg aagctgatac gaaagttgtc acgatggcag cagtcgaacc
    27061 agaagctgca gaacaaacag ttgaagaaca aggatcagaa gaataaccat caagactaag
    27121 tgtgtaagct agcaagaata accgattaaa aaatctggtt attcctgctg gcttttttga
    27181 ttagttgaat ttctcttaaa atattcgaaa ctgttcattc tgtattgctt ttgaccaaaa
    27241 tagagtgtat aattagagct tgtgagtagt ctaattaatt agataactct ccttgctctt
    27301 aggaacctaa gagccgaaag tccacaggga ggtgaatatc aatggaaaac acgaaatatg
    27361 aaatcatgta cattattcgt ccaaacattg atgaagaagc aaaaactgct ttaatcgaac
    27421 gtttcgatgc aatcttgaaa gataacggag cagaagttat cgaatcaaaa gattgggaaa
    27481 aacgccgttt cgcttatgaa atgaacggtt accgtgaagg tatctatcac atcgttaaag
    27541 tatcttctcc atcatcagca aatgctgtca atgaatttga ccgtcttgct aaaatcaacg
    27601 acgatatcat tcgtcacatg attgttaaag aagaagctta attgttgttt catgtgaaac
    27661 aatagaaaac gatgagagga gatgactatt ttgattaata atgttgtatt agttggacgt
    27721 ctgacgaaag atccagattt gcgttacaca gcaagtggta cggcagtcgc aacatttact
    27781 ttggctgtga atcgtaattt tacaaaccaa agcggcaatc gcgaggctga ttttatcaac
    27841 tgtgtgattt ggcgcaaatc agcagaaact ttagcaaact atgctcgtaa aggaacactt
    27901 ttaggtgtta ctggacgtat ccaaactcgt tcttatgata atcagcaagg acaacgtgta
    27961 tacgtgacag aagtagtagc tgacaacttc caattgctgg aaagtcgttc tgcatctgaa
    28021 aatcgccaac aaagcggcgg attccaaagt tctggtcaat cagctggcgg atttggcgga
    28081 aacaataact ctaatcaaac atctcaatca tccaacggta tgcctgattt cgaccgtgat
    28141 acttctgacc cattcggctc aagttcaacg atcgacattt cagacgatga tttaccattc
    28201 taaacgaatt tgataggagg gaaatagaat ggcacaacaa agaagaggcg gacgtaaacg
    28261 tcgtaaagta gactacattg ctgctaacca tattgaatat atcgattata aagatatcga
    28321 attactaaaa agatttattt cagaacgcgg taaaatcttg ccacgtcgtg taactggtac
    28381 aggcgctaaa aaccaacgta aaattactgt tgcaatcaaa cgcgctcgta tcatgggctt
    28441 gctacctttc gttagcgaag actaataaat cctaggaatt gaatgactga tccttttgat
    28501 cagctattca attccttttt ttctgtaaaa ataacctcta ttcgtttagt cgctcgaatg
    28561 acgaaagtta tggtaaaatc aaattagtca tatatgggag gaaaattatg gaaaataaaa
    28621 acaatcattt atccccgaaa cctctcttcc ttatcctgtt tatattaatc gaagtattgg
    28681 tagtcttaat tttaccattt cgttttgtta gcgcttcggt cattctattg atgaatttat
    28741 ggggtgttcg ctattggttg aaaatgaaac gttggcttat tttaaataaa cgtgaaaaaa
    28801 tccagcaggc ttcgactgta gctgaaacca atctagctta tgtctcagaa gtcatgcctg
    28861 taggagtgat tgcttatcag ccatcaagcc aaaaaatcga atggctcaat ccattagcat
    28921 tgaaaatcaa agatcaaagt ggattaacag aggaagaatt gctagatact ttttttactg
    28981 caaaagaaaa aaagaaaaac caagtctatc tagcagataa aacatttcag tttgatatgg
    29041 atacagtaaa gggtgttgtt tattttaatg atattaccca aaaccagttg ttaaaactcc
    29101 atcagacgaa cagtcagccg gtgattggta ttttatctat tgataactat gcagatgcca
    29161 ttgataaatt agatgacaaa gagatttctt atttgaactc tttcgtcact acactagttt
    29221 ctgactggat gaatgaatac catgtctttt tcaaaagaat caacgcagaa cggtttttct
    29281 tcgtagcaca gatgatcgat ctgaaaaaga tgatggataa aaaatttgat ttgctggatc
    29341 gctttcgagc agaagctgaa aaacagagct cgcctatcac ccttagtatg gggattgctt
    29401 atggctcaag aaccctcgat gaaatcggtg gagaagctca gaataattta gatgttgctt
    29461 tagtccgtgg aggcgaccaa gttgtcgtga aagaggtaaa agaaggagca aaacctgttt
    29521 attatggggg gaaatctgct aagaatgtca aacgcacgcg tgttcgttct cgagcgatga
    29581 gtaccgcttt gcgtcggatt tttgatgaaa caggcgatgt gtttatcatg gggcacaagt
    29641 ttccggatat ggatgcttta ggctctgctt ttggtgtcag ttgtctggca cgcttcaacc
    29701 agaaaaaagc ttacatcgtt atcaatgaga acgagatcat tccagatgta gaacggtgtc
    29761 ttgaagaaat ccataagcat cctgaattag aaatgcaact aatctctcca gaacgtgcca
    29821 tagagctgaa aaaagaagag gacctcctcg tgatggtgga ttaccataaa ccttctctat
    29881 cgatctctca gggcctatat gagcaatttg ataagattgt gatcattgat caccatcgtc
    29941 gtggggaaga gtttccaagt aaacctctgc tgacttatat tgaatcttca gcttcttcag
    30001 cttcagagct agtttctgag ctgatccaat accaaagcag caaaacaaat cgcttagatc
    30061 gttttgaggc cacattattg cttgcaggga tggttgtaga tacaaatagt ttcaatgtcc
    30121 gtacaactgc acgaactttt gacgttgcca gctatttaag gtcttgtgga gcagacgcat
    30181 ccttagtaca atatttattg agctcggatt tagcgacata cttggagatc agcaatttga
    30241 ttgctggaag cgactatgtg acttctgata ttgttgtggc ggcagggaca gacgaaaaag
    30301 agtataatag tgttatagca gcaaaaacag cagatacatt gctttcgatg atcaatatca
    30361 atgcagcttt cgtcattaca aagcggacag acggcttgat tgggatcagt gccagaagta
    30421 ccggcacgat caacgtccaa atcatcatgg aatccttagg tggcggagga cacttcacaa
    30481 acgcagctac tcaactggaa aatacgacag ttgaggaaac aaaacagaag ctatttgctg
    30541 caatcaaaga aaatatgaat caaatatatg ataaggagta atccaaatga aagtaatttt
    30601 tttacaagat gtaaaaggaa aaggtaaaaa aggggacatc aaagaagttc cagcaggtta
    30661 tgcacaaaat tatctaatca aaaataactt ggcaaaagaa gcaacgaaaa gcagtatcag
    30721 tcagttagca ggacaaaaga aagcacaaga aaaacacgaa gctgaaatct tggaagaagc
    30781 taaaaagtta aaagaattct tggaaaaaga agatactgta gtcgaattga aggcgaaagc
    30841 aggggaagac agccgtttgt ttggttcgat cccttcaaaa caaattgctg aagggttaaa
    30901 caaacaatac aaaatcaaat tagataaacg aaaaatcgaa ttagaaaatc cgatccgttc
    30961 actaggctac acaaacgtac ctgttaagct tcatcatgag gtgacggcta cgatcaaagt
    31021 tcatgtcgta caagaataac aaatcttgat agcgatgttc agctggtaag tataaccaga
    31081 aggaaaaaag acaaaccgaa atttatctga gaaaacagct gcagcaatga aagcaaaagc
    31141 tgcaaatcga tttcagattg atttcggttt cttttttggg aacagcaaaa tagcataaag
    31201 aaaacgagta taataaaaga gaactatttt tttattacac tttcggtacg aatgatgact
    31261 tttctctcat tttcttgtaa aatgaagcaa ttgaagacaa ggagcgatac gtatgaatga
    31321 agtgtggcag gatcgaatcc caccgcaaaa tattgaagct gaacaagcgg ttttaggctc
    31381 tgtttttctt gacgcagaaa cgatcattga tgccatggag tacatagagc caaaagactt
    31441 ttatcgtcgt ggccatcaaa ttatttttga gacaatgatc gagttaaatg atcgtaatga
    31501 agcgattgac gtggttactg tcaaagaccg gctggaacaa gccaatttgt tagaagatgc
    31561 cggtggttta agttatctct cagacttggc attagcagtt ccgacagcag caaatatcgt
    31621 ctactatgcg aaaatcgttg aagaaaaatc attgctgcgt acattgatcc agacagcaac
    31681 aggaatcgtt acgaaaggat tcgagcaagg agaagaagtt caaacaattt tagatgatgc
    31741 cgaacgcagt atcttggagg tttcggaaaa aagaaatcga agcggctttt tatctattgc
    31801 tgaagtactg aactccacga ttgaaaatat cgatcgacta gctcaaaaca atgaagaaat
    31861 cacaggtcta ccaactggct atcaaggtct ggataaaatg acagcaggtt tccagcccga
    31921 agaactaatc attcttgctg cacgtccagc ggtaggtaaa acagcgtttg cattgaatat
    31981 tgctcaaaat gtaggaacaa aaacagatcg ttctgttgcg atatttagtt tggaaatggg
    32041 ggcagaatct cttgtaaatc ggatgctttg tgcagaaggt tcgattgagg ccagccattt
    32101 acgtacaggt cagctttcgg aagaagaatg gtcgaatctg atcgttgcga tggggagtct
    32161 ttctcgagca aatattttta tcgatgatac accagggatc aaaatctctg aaatccgtgc
    32221 gaaatgtcgg aaactagcgc aagaaaaagg caatctaggc ttgatattga tcgattattt
    32281 gcagttgatt gaaggtaacg gtaaagagaa ccgtcaacaa gaagtatcag atatctctcg
    32341 ccaactgaaa aaactggcga aagagctgaa agtacctgtc attgctcttt cacagttatc
    32401 tcgtggagta gaacagcgac aagacaaacg accagtgcta agtgatattc gtgaatccgg
    32461 atcaattgaa caagatgcgg acatcgtagc cttcttgtat cgagatgatt attatgaaag
    32521 agaaggcgga gatgacgatg atggaaacga gccaaaaaat gacaacgtca tcgaagtcat
    32581 catcgagaag aaccgaagcg gtgcacgagg aacagttgaa ctactgttta tcaaagaata
    32641 caataagttt tcgtcgcttt cgccaagaga agaattttaa agaagaggtt gtgagccttg
    32701 agcaatcccg agagctctcg taagaagatg caaggagtaa ccgcaggaac gactagagta
    32761 cttaatttaa acagatctcc aaagggattg tgacaaaagt cttgtcacag tccctttttc
    32821 cgaataaacg gtgttcaatc agctgaccct tggtattagc tcagcaaacg tcaaaatatg
    32881 aggaactatt ttgacgtttg tttctctaat actcaggtca aaacgctgat cttacgacct
    32941 cttttcctta aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatcatcaaa
    33001 catgagaagc agttttttga aattgctcct tgtgactcag agttaaacgg caggctcaca
    33061 acctctttct taaacggtgt tcaaaagctt gcatctgcgg taatacgccc aatcaaacga
    33121 aaaatatgag aagctatttt tgtttggctg ttcttattac ttgatgcagg acagcttttt
    33181 cacaacctct gattcacggt gttccaaaag tagcttcttc gaaaaataag tcgaaatttc
    33241 acaaaaattt gagaagcaat tttcgtacat tccttcttat ttcttgaagc tgaccacttc
    33301 tgttacaacc tcttttttaa taaccattct aaaaagataa agttcgtttt ttaagcgttt
    33361 ttataaaata aaaataaaaa tcattcgatt ttatttgaaa tatgtatcta ttattgatac
    33421 aatggttcag gattaaataa tatagaacga ggtgttcaca tgtcatctgt agtagtagta
    33481 ggtacgcaat ggggcgatga agggaaagga aaaatcacag actttctcag tgaaaatgct
    33541 gaagtgattg cacggtacca aggtggcgac aatgcagggc acacaattaa gtttaacggt
    33601 gtgacttata aactgcattt gattccttct gggatttttt ataaagacaa gatcagtgtg
    33661 atcggaaacg gcgttgtagt caatcctaaa tcattggtta aagaattagc ttatttacat
    33721 gaaaaaggtg tagaaacaag taatttgcgt atttctgacc gcgcgcacgt aatcttgcct
    33781 tatcatatca aattggacca attacaagaa gatgctaagg gcgaaaataa gattggtaca
    33841 acgattaaag gaatcggacc tgcctatatg gataaagcag ctcgtgtagg tatccgtatt
    33901 gcagacttat tagataaaga aatttttgaa gaacgtcttc gtttgaatct agatgacaaa
    33961 aaccgtcaat ttgtaaaaat gtttgattca gaaccacttg attttgacga tatttttgaa
    34021 gaatattatg aatacggaca acaaatcaaa caatacgtca cagatacttc cgttatcttg
    34081 aatgatgctt tggatgcagg caaacgcgtg ttgtttgaag gcgcacaagg tgtaatgctg
    34141 gatatcgacc agggaactta tccttttgtt acttcttcta acccagtagc cggtggggta
    34201 acaatcggaa gcggtgtagg gccttcaaaa atcaataaag ttgtcggcgt ctgcaaagcc
    34261 tatacgtcac gtgtcggtga tggaccattt ccaactgaat tatttgatga aacaggagaa
    34321 caaatccgcg aagtaggacg agaatacggt acgacaactg gtcgtccgcg tcgtgtaggc
    34381 tggtttgatt ctgtagttat gcgccattct aaacgagttt caggtattac caacctatct
    34441 ttaaactcga tcgatgtatt aagtggattg gaaacagtaa aaatttgtac ggcttatgaa
    34501 ttagatggcg aactaatcta ccactatcca gcaagcttga aagagttgaa ccgctgcaaa
    34561 ccaatctatg aagaattgcc aggctggtca gaagacatta caggatgcaa aacacttgca
    34621 gaactgccag aaaacgcacg taactatgta cgtcgtatct cagagcttgt tggtgtacgt
    34681 atctcaacat tttctgtcgg acctgataga acacaaacaa acatcttaga aagcgtctgg
    34741 gcgcaaatct aaggataaaa acgacgactg aatcaggacg ttcttaccag agcgagtcga
    34801 agtgtttatc tatacgataa taataacttg aagaggtagt gaaagagacg tttagcctta
    34861 ttgccgatag gctgtctctt gaacactgtt tattcagaat aagggactgt gacaaaaacc
    34921 gtgtcacagt ccctttttcc gaacaaacgg tgttcaaaag cttgcatcta cggcactaag
    34981 cccaatcaga tgaaaaatat ggagtgctat ttttatctga ttgttcttac tgcttgatgc
    35041 agagcagctt tttcacaacc tctccactaa acggtgttct cctgccaact cctcggtcac
    35101 aagtcacaat attcaacaaa catgagaagc tgttttttga aattgttcct tgtgactcgg
    35161 agctaaacgg caggctcaca acctcttggt aaacggtgtt caatcagctg accctcggta
    35221 ttagctcaaa aaacggcaaa atatgaggaa ctattttttc cgtttttttc tctaatactc
    35281 aggtcaaaac gctgatttca caacctctga ttcacggtgt tccagaagta gcttctacgg
    35341 aaataagccg aaatttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc
    35401 ttgaagctga ccacttctgt cacaacctct tattttgatt agaacttgcg aaaataaacc
    35461 ggttaaagtg tgtctagcgc aagagatttg ctataataga cgataagggt aaaatgtaac
    35521 gagaaggggt tgaaaaatgt gtatcaaatt gtaacagatt cttgttgtga tctgccatac
    35581 catgtattag caaaagaaaa agttgatttt ttatcaatga atctccaatt gaatgatcaa
    35641 gagttagtag atgacctcgg aaagacattc aattatgacc gctacatgaa tcagctgaaa
    35701 aatggtgcga tgcctacgac atcacaaatc aatgttggcc gatatttaga atttttccgt
    35761 tcttatgcgg aaaaacagat tcctgtttta tatgtggctt tttccagtgg attaagcggc
    35821 tcttacagta gtgcgcttca agcggttgaa ttgctgaagg aagaatatga agaccctgag
    35881 atctatgttt ttgatacaaa agctgcaagt attggggaag ggcttttagt attggaagct
    35941 gtaagattga aaaaagaagg taaaagcatc gaagaaaccc tagagtggct ggaaagtaat
    36001 tatttgaaag tccattcatg ggtaacagta gacgatctga aatatctaga acgaggcggt
    36061 aggatttcca aagcaagtgc tactcttggc agtctattga gtgtaaagcc tattattacg
    36121 gtggataaag aaggaaggct gacgaatgtt gacaaagttc gaggaagaaa caagtcgatc
    36181 cagacaatcg tcaatgagac ggtaaaaaca atcgttgcac cacttgatca aatcgtttat
    36241 gtggcttacg ctggggatct agagagcgcg gagaaggcaa aacggctttt ggaagagcag
    36301 ataaagatga aagatgtgaa gctttatccc ttaggaccaa cgatcgccag tcacacaggc
    36361 tatggctgta tcgcgatttt ttcgatggga atcagtcgat aaaagcagta actcattctg
    36421 taaaaaagcc cagaaattga ttgtaacaaa aacagcgact cttaaaagat agatgagcat
    36481 cctatctctt agaggtcgct gtttttttat tttttaagct aaataggcca ttatgcccat
    36541 aactcgttca attgtttttg aatatctgca ttttccaaaa attcatcata tgtcgtatca
    36601 gctcgatcga tcacaccttt atccgaaacg gcaatgatgc ggtttgccaa tgtttggata
    36661 aattgatggt catgtgacgc aaataagatt gagccagtaa atgccatcaa gccgtcattc
    36721 aatgctgtga ttgattctaa gtctaaatgg ttcgttggat catctaatac aagtacgtta
    36781 gctttcgaaa gcatcatttt tgaaagcatg caacggactt tctctccacc agataaaaca
    36841 ttcactggtt tcaatacttc ttcaccagaa aacaacattc ttcctaagaa gcttcttaag
    36901 aaggtgttat catcttcttc tttacttgca tattgacgca accagtctaa aatcgttaga
    36961 tcagtatcga attctttcgt tgtatccttt ggtaaataag aattacttgt tgtcacaccc
    37021 caacgtactg ttccagtatc tggcgtaatt tcacccatga taactttgaa tagcgttgtt
    37081 gttgttatat cattttctgc gacgaaagcg accttgtctt ctttgctcaa attaaaagat
    37141 atatcatcta aaattttctt tccatcaatc gttacactta cattttctac ctgcaataag
    37201 tcgtttccaa tctcacgttc aggtttgaat ccaacgaatg gataacgtct agaagaaggt
    37261 tggatatcgt ctaaagtaat tttttcaagc atttttttac gtgaagttgc ttgttttgat
    37321 tttgatgcat tcgcactaaa gcgggcaata aattcttgga gttctttgat ttgttcttct
    37381 tttttggcat ttgcgtttgc ttgaagttta gccgctagtt gactagattc caaccaaaaa
    37441 tcataatttc ctacataaag tttgatttta ccaaaatcta gatccgccat atgtgtacat
    37501 actttgttca aaaagtgacg gtcatgggat acaacgatca cagtattttc aaaattgatc
    37561 aaaaattctt ccagccaatt gatcgattgg atatccagac cgtttgttgg ttcgtctagc
    37621 aaaagaacat caggtttacc aaatagtgtt tgtgctaata acacttttac cttttgtcca
    37681 ccagttaatt cactcatttg ctgatggtgc aattcttcag ggatgttcag accttgcagt
    37741 aaaacagctg cttctggttc agcttcccat ccattaagct cagcaaattc tccttctaat
    37801 tcagctgctc gaataccatc ttcgtcagtg aaatcctctt tcatgtaaat cgcatctttt
    37861 tctttcagca cttcatacag acgcttgtgt cccatgatga ctgtttcaag tacagtatag
    37921 tcttcatagt cgtaatggtt ctgtttcaat acggccatcc gttcatctgg tcccatagta
    37981 acggaacctg tggaaggctg cagatcccct gataacactt taaggaacgt tgatttacct
    38041 gcaccatttg caccaatcaa gccatagcag tttccggggg taaatttaat attcacatca
    38101 tcaaatagtt tgcggtctga aaagtgcaaa ctcacatcat ttacagtaat caattttttt
    38161 cctcactttt atcattatta gttttgacaa gtcttataga attatatagg ggaaaggtaa
    38221 aagattcaag gcaatcgaac attctttgtg tttcacgtga aacagaatga aaaaaataaa
    38281 aataaaagcg gagcgaaaat agctactata ggatttatta gtttgcaaca gcataatttt
    38341 tgacgttatt tataagaggg agtactataa aactgtaaaa gaaatgaaag aaggattgaa
    38401 aatggatgaa aaaagaacca tcgactgggg ctcattgatt ttgggaattt tatttgtact
    38461 tgtctcattg ttatcatttc aagaccctgt cggaaattta gttgcaattg tagtagtctt
    38521 tgcgattttt gcctttatta aagggatatt tgaactgttt gtacggaaca gattaaaaga
    38581 gctaacagga tataaaggaa agatgccctt aataatcggg accatcgata ttctagttgg
    38641 cgtctttttc ttattcaata ttggtgcagg agtcgcagca ttgccttttg tttttgcagt
    38701 atggtttatt gctgattcag ttttagcgtt atttactgct gacttggcaa gaggagtaag
    38761 tgaaggatat tattggttta cgatcatcgt caatatttta ggaattttat taggaatttt
    38821 cttattgttt aatccaattt cttctgcgtt gacattaagc tttttagttg gattctattt
    38881 tatgttattt ggtatcacac atatcgtcta tgcatttaga taagtaaaat caaacaaata
    38941 aaaaagccgg cttccgatta cagttttact gttgtaattg gaagccggct tttttatcat
    39001 ggacaaatat gcacgaggtg atttttaggt gtcgagacaa atttatgcaa aaatgtgaga
    39061 agctatatac acaaatttta tcttaatact cgaagcatag cgcagttgtc acaacctctt
    39121 ttcatataat tcattcaata ctggcaaatc atggacttca tatttttctt tgaatgcttg
    39181 gatttcttct tcagaatatt ttcttggatc taaatgcaga tcttctactg gcaatgggcg
    39241 cttgttattg atcttcacat cgattactac aggacgatct gattttgcag ctgcatcaaa
    39301 ggcaaatcga agttgcgagt attctgtgac tgtaaagcta tttgccccga gcgcttctcc
    39361 aactttaccg aaatctgcat cttctaaaaa tacaccaaat ttcttttgtt ccgtatcttc
    39421 ttgttctgct tcgatgaaac caaaagaatc attcgaaaag acaacattaa taattggaag
    39481 ctgatatttg acttgtgtaa tgatatcttg catgaccata gcaaacccgc catcaccact
    39541 taaggtaaat acttgttttt ctgggtagct tagttgtgcg gcaattcctc ctggcacacc
    39601 attacccata gttgcgaacc atccagatgt cgtatgtttt tgtttaccgt tcatattcaa
    39661 taaacgaata gaatggatag tcgtgttacc gacatctgtg acgaaaatcg catcgtcttc
    39721 agcaatacga ttgatttctt tatagacagc ctctggacga atcggttctt cctctcgatc
    39781 ttcaaaagat ttcaaccaat ttacccattt ttctttattt tcttgatttg ctaggtacca
    39841 gctatcagca gggcgagcat cccctaactc gacaagtctt tgcaaagctg tgtttgcatc
    39901 accgaggatc gacaaagatg tttcatggcg tcgtccaaat tttgacgcat cgatatcgat
    39961 ttggataaat tcagcagttg gattaaaaaa tgcacgtcca aatgggaagt cgctaccgac
    40021 aaataagatc agatcggctt ctgccaatgc ttcatttccg ggtttagtag cgacacgtcc
    40081 agcaaaacca agaaagtttt cataatcatc aggaataatt ccttttgcta gaacggaagc
    40141 gacaacagga actgaaaaat gttctgagaa ttctttgatt tgcggaaatc cgtttcgcgt
    40201 tccttgacca acataaagaa ctggtttttt tgctttttca agataaggca aagcagctag
    40261 caaatctttt tcttctggta atatcacact tgttttgtga gtgtgagcgg tggagaaagg
    40321 ttgttcgtca atttcttcaa aaccaagatc aacagggata gtcacgatag ccacgccttt
    40381 atgctcatag gcggctttaa tggcttcatc tactacatgc ggaagacttt gtggtgtcat
    40441 gactgtacga ttgtatacgc taacatctgc gaaaatagga ttttcattta gttcctggaa
    40501 agagttatag ttcatggatg tagaagcaac ttgtcctaag aacgctacta gaggcacatg
    40561 gtccatctgt gcatcataca aaccattgat caaatgtgtc gcaccaggtc ctgcagagcc
    40621 gaagacagcg ccgactttcc ccgttaattt tgcatctgca gcggctgcga gtgcgccgac
    40681 ttcttcatga cgaacttgaa tgtagttgat ttcggttttt tcttgatata gcgcatccat
    40741 aattgaattg aaagatcctc cgggaatgcc gtaaatatgg tcgatacccc agctttcaag
    40801 aacttttacc attgcgattc ctgcattgat ttttgacatg gtgtttcact cctttagcaa
    40861 tgatgataca taaatttttt gtatctagag tatagaactt tttatcgaaa atcccaacta
    40921 attcgtcttt tcacaaaaat gtagtcatgt ttttgggaat atgctagagt agtacataga
    40981 tagattcaag agaggggaat ggcatggttg aatggctatt gagacttgaa ccttggcagc
    41041 aagcacttgc tgggacagct tttacttatt ttatgacagc tcttggtgcc gggttggttt
    41101 ttttctttaa agaaatcaaa aaagaagtgc taaacttgat gttaggattt gcttctggtg
    41161 tcatgattgc tgcaagtttt tggtctttgt tagatccggc tattacaaaa gcagaagaaa
    41221 atggagatat tgcttggcta gttgtaagta ttgggtttgg tttaggcggt gtgtttttat
    41281 atatggcaga taaaacactg ccccatatgc attttggacc acaacatgaa gcagaaggat
    41341 tgcctaccca tttaaaacgg acgatcttgc ttgttttttc tattacattg cataacattc
    41401 ctgaaggttt agccgtgggg gtagcattcg gtgcagcagc aactgcggat aatccgacag
    41461 ccgctatatt agccgctgtt tcggtggcgc taggtattgg tatccaaaac ttcccagaag
    41521 gagcagctgt atcaatcccg ttgcgacaag aaggtctgag tcgtaaaaag gcctttgttt
    41581 acggacaagc ttctggaatc gttgaaccaa ttgctggagt gattggagcg ctactagtaa
    41641 cgaaagtgga gctgctgctt ccttatgcac ttgcttttgc cgcaggagca atgatttatg
    41701 tcgtggtgga agaattgatc cctgaagcac agcagacatt gtccagcaaa cgtcattttg
    41761 cagtttttgg cgtcatgagt ggatttatca ttatgatgat tttggatgtg gcattaggat
    41821 aaataaaaaa atatatatga tatatcaaat agggccgcga acttatatcg cgaccctatt
    41881 tttttagtaa gttatatcat aatgaagtga atggtatcat acattaataa tagttttttc
    41941 tatgtgtatt tcctttgatc cagcgaacaa gccagatgat aagtgcgatt gggattagaa
    42001 ctttgaccgc aaaccataaa agcccgctta ggacactgac aaaaagactg atgacgaagc
    42061 tagctgcaat tagcactaaa agaaccatga agatatttaa gaacatattt ttttgacgca
    42121 tacaaaaccc tcctcttgtt cattctttac tatagaaaaa gtataacagg gattgtttaa
    42181 aaacacctcc aactgaagac tgatttttac ttcatctact taaatgatca aacaattgct
    42241 ttttctctgg tttatcatct aaaatagagc ggaaaaggag gagaatatca tgcctaaaaa
    42301 tcgtcttgaa gcatttacag atgctgtaat cgcgattatc atgactattt tagttttaga
    42361 acttcatgaa cctaacggag acacgtggga ggcgctcgct gcattaggtt accgattttt
    42421 tatctatatt gtcagtttta tcattttagc gatttattgg aacaatcatc atcatttatt
    42481 tcaagttgta cataagataa atggacgggt cttatgggcg aacagtttct ttgtttttgc
    42541 gctttcgctt tttccgtttg caacttcatg gatcagtgag tacatcaata gcctggctcc
    42601 agaaatgact tatggttttg ttaccttgac cgctaatatt gcgtattata tcttggcaaa
    42661 agaacttgtc aaagcaaata aaggccacga gaaagcattg aaggcgatgt tgaataatta
    42721 caagaaatcg tatctatcga ttggtttgaa tgtgttagga attgtcttag ggtatttagt
    42781 agcgccagta gccgtgctga tcgcaaacat cgtgattcta gctgcttgga tcatcccaag
    42841 tcgtaccatc gaatcacaat atcggtaaaa tgatccgtgt ttaaaattta agagaaaaat
    42901 atagaaaaaa taaataaacg atcaaatatc cgagtgatac tagtattcaa agagggatgc
    42961 agatggatca aaaaagaatt catgtaggac tgattactag ttttgtcacg atgcttattt
    43021 atttggatca aggtcatttg acttggacag tagttggtgt cgtaggattt ttgacattat
    43081 tcacagcaat aaaaaaataa acatctttat gtaatctgat gttctatcta tttaagaaaa
    43141 gcatgtttac agcagtatga ttctgttgta aacgtgtttt tatttgtgga aaatttaatt
    43201 tttagaaaag tgtatcgaaa aatagatagt tagtttttcc acagtggata acttcgtaag
    43261 agataaaaat aaaaaattag tcaaagaagg gcgtatagat ttcttttgat aggagatata
    43321 caacagaaat gaataaatat taacgtgttt tcaaacagta taaaacggtg tggtttttgg
    43381 aataaaaaaa taacatatct cattttacac ttttgttatt ttatacttaa aaaataacga
    43441 cacagaaaat accgtgtctc agatttctct actacttcta aaaactactg tttttttcct
    43501 ttgaaatgcc ttcaaaaagt aggatcaggc agaatatact ggcagaatca aggagaaggg
    43561 agcctaaata aggagaaaaa ccatagaaaa tataaagtaa ttacttataa aacaagcttt
    43621 ttatagaaaa ataatatacc tgagtagggt ataaattaag acggaattcc tttctagatt
    43681 gagatattga ctaggaagaa tgttaagatt ttaagccttt aagctaaaaa aaaacccttt
    43741 ttgttaaata aggggaatat agcctgtcag tttcaaactg caagtcgtcc tgctgcaata
    43801 atatagtaga ttagagatga aaatagattt ttttaaacca atgactgaat aaaaagaccg
    43861 tcatagatac acattggaat gtgtctatta cggtcttttt ttacgaacga ttttattgaa
    43921 gacggacagt tcgggtcaaa aataaaaaag ctacgatcag aaataagaat aaaatgtatc
    43981 ccaaaataat ggctagacgg tctaacttat ttgtcgatag atgtttgttt agaagttgat
    44041 aggcccataa acctatcagt cctcctgttg tattagttat caaatcagtg atgtccgcta
    44101 cgccaatacc gaataggaat tgaatcaatt ctgccaagaa gcttacaagg aaaataatcc
    44161 ctaatttttt agaaaaagaa agtctcttga aattgacatt caaaagcacc ccaaaaggaa
    44221 aaaagacaat caagttataa gtggtctcac gtaataccgt cgtattttca aaagaaaaag
    44281 tagaaaaagg gacgagattg acattgctgt aagaataagt gagaaattgt ggaagattaa
    44341 atgatagctt gaataaaatc aaccataata gaaagaataa ataaatccct aataaaaatt
    44401 ttgatacata ccgcatagac atctcctcca tttaaaaagt ataccatcga aaaccgctga
    44461 aaaatcataa aaaagtatga agtttaagga ccaacgtagt tatcgtacga aaattagaag
    44521 cttttttttg ttgggaatcc ggtttcttta acaaagctaa gatagcgagt tgcttggaaa
    44581 agttgctatc ttttttctgt caaaacgact cggaatgttc gttttttgtt ctaatttctc
    44641 caagttgttt actttttgat actaagttta tgaaaaatca agaaatagtt gttatactag
    44701 gtaatgctga caaacgggat tggcatttca tcttggttca gctttttttc caattactag
    44761 gaaggaggga tagattgttc aagacttatt ttcgtttcaa taaaatacaa gataatcctt
    44821 ttcgtatcat gaatctgatg ctacttatct ttattgtatt atggttaggt tacataaatc
    44881 atgatttttt gatttcaagt ttttcatcat tagggatctt tacttttttc tattatcaaa
    44941 acataccgat gaaatatttg ctgaaacgta tgtttctgat cggttgcgga ctgttggttg
    45001 ctttcgtttt ggggatctta tcgacttatg ttttatggct tgaaccattt gccgtagctt
    45061 tggttgcttt ttccagcaga ttcgtgttgc gattattcca tatttctaaa ccaggtggct
    45121 tgttttttgc gatgctttct gccatgggaa cgagcatgca gctgccaatc gctcaattac
    45181 cgatcgtctc actttatttt tttatgggtg tcgtttttgc cctgattgcg gcagtgatta
    45241 caaaattact ggattcacgg cctgagcaga caattgaaaa agcaacttta aaagaaagat
    45301 ttcacgaaga gcctttagtg atcattgatt ctgttttcta cagcgctgcc ttatttcttt
    45361 ccgtttatgt cagtcacggc ttgaatctgc ataatcctta ttggttgacc ttgtcatgtg
    45421 catcgatttt gctagcagaa aacttggatg cgatgaagca tcgacaagtc caatatctga
    45481 ttggatcgat gggtgggtta tgtgtgtcgg cattcttgtc atttgtgccg tttacgcagt
    45541 tgcagaccat ttttctgatc acttttctat atggaatcgc acaatttcta gttgctagaa
    45601 attatgcagt tgctaatatt tttcttaatc cgatggcgtt gatgctatct acacttattc
    45661 gtggagcata cttgatttct ttgatcgaat atcgtttttt agggatcgtc ataggaagtt
    45721 tcatcggttt aggtgttgcg tgggtaatga cggttggttt gcaacattat ttaacggttg
    45781 ttcgtcaaaa attagaatag aaggacaaat atatgtgcca aaagaggtta tgacaagatt
    45841 tttgtcacaa cctctaattc aaggtgttca aaagccagat tctcagcatt aagtcaaatt
    45901 cacacaaaaa tgtggaaagg aactttcaaa aaaattttat atttattcta tcaaaaaatt
    45961 ttgtttatga tggtaactga ataaactttc ctgagatggt caatgctttc cttgaaagtc
    46021 ttacagatat tctttcttca tagaaagttt aagttttata gttagtgtac aataaaagta
    46081 tgtttatacc atatggtgtc cggataatca gtcatttaaa caatgaggtt caaagatatg
    46141 aaaaggggaa atttgatgaa gaaaataagc aatgaaatcc aaattctggc agaaacttta
    46201 actcttccca aagtcgttat agagcaaatg attgtacaag caaaaaacat ccttgatgag
    46261 gtgtttgagc aatatagtca tgaactaatg gatcctaata aagcaaaggc gactgcaaag
    46321 aaaatagaca aagtctatga agaggaacct tttgcagcat tagctattta tctatatgca
    46381 gcttctcaaa gttggaaaaa aatttatcaa ccattgaaga ttccaagaac tatctatttg
    46441 gcaacaatga atgcttttac tagatttatt catgaacatt tccaagtaac aggaagctat
    46501 cgctttgacc gtggtttttg gatttggcat tacactagtg gcttgatctt tcggattggt
    46561 gaattggagt acgaaatgac atattttccg aaagggtaca cggatctaag attagaagga
    46621 aagcagtgcc tttctcttca tattccttcc gatgcagatt tatctcagaa taagataaga
    46681 gatagttacg aaagtgccat tcgttttttt gaactttatt ttcctgatta ccattaccaa
    46741 gtgatgtaca cagatacttg gttactatca cccaatctta ctaaatggtt gaaaaaagag
    46801 tcaaaaatct gtctttttgc agccgattat cgattattat cagttgatga acaggacgat
    46861 agtggagtcc cttggatctt tggacgagta gatgcccaaa tacatgatta tccagaaagt
    46921 acgagtttac agcgacaagc aaaagagcaa ttgcttgcag gcgagcacat tggctctggc
    46981 ttaggtattc ttacttcata agtttagtac ttcgaaatag tttaaaagag cgtgagacaa
    47041 aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagcaa
    47101 ctccttcgga aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaatttctt
    47161 cttatttctt ggagttaaac gcttctgtcc cagcctcttt tttagtgttc tatcgaacac
    47221 acagtacgtt caataggtaa actagctata acaatagata ttaaagttaa cggacaagag
    47281 ggcctttttt aggtagctaa aacgtaataa tgaaaattta tttccagaga taggagtcaa
    47341 tcagctagtc tgatcgactg ttttaacaag ttgactttca ttttaacttt tgataccatt
    47401 taaaacacaa atcgtaattg tttcgatttg tgttttttag catgactaca atcaaagaag
    47461 aaagtgggtc gtccatatgg aaaaaacaga tatctcaagt gcataccgcc gcttgaaaag
    47521 cccaaatatc aaaacacgaa aacgtgcgct gaagatcatc aaagatgtga aacggaatag
    47581 tgggaaaagg taagaagaag gaaacttaat taaaaaaata gccgaaaaac gtttttcggc
    47641 tattttcaat aatcggtttt tgtgtaaggt ttccgaaaga actgctgttg attttccatt
    47701 tttaacatat gataaattgg tagaatcttt cttttcaaat tgataccatt ccctaattcc
    47761 agttatatag ttaactacag aaccacaaag cacgtacgtt tataaaagta ttatctgtag
    47821 ttatttttca attccctttc aacctcttat tttgaccagt agtattgatc actttacctt
    47881 ttaacacctt tgttcgtttt tgaaacattt ttagtcaaga atggaaacgt aactacataa
    47941 tcaaaggttt tgcgaaatat ttcatgaaat atttcatgta gaagcgagca atacggttgc
    48001 aaataaccat tatcgtttaa taagcagtta cgaaagctcc caatatctct aacgttcagc
    48061 tcactccatt tcttttcttc aaaaaaatca gtgattcgta tctctttcta tttgtcgaaa
    48121 aagttaactt taaaaagtaa cgccgaaaaa atatgcaaaa aaataaaaaa gcagttacta
    48181 ttgctagaat aacatcaagg aataataagt tttggttttc agataatagt cgataagttg
    48241 tgtattggaa ttttagatac agtaataaag tatttttcat gaaattttgt gagaatcact
    48301 attacaagta tctttaataa aattaatggt taaggaggaa cttaaagtag aatgtaaatg
    48361 aatagttgta gtcagtcgat tgtgatacta gactcaaagt tgcaggaata agtttttcaa
    48421 tttgtgattg gaagataaaa aatgaaaaag aagggctttt ttttatactt tgttgattgt
    48481 agtgtttatt ttccctctgt ttagccaagt aacattttct atgactcaac cagtttcaag
    48541 tgaggaacat gataaccttt ttcgtgctac agaagagttt aagaaatatc aggaaaatat
    48601 tgttccggag actccattag ctagtgagcc aattactgat attgatggaa accaaatagg
    48661 atggtatata cagtacgaag ttatggtggt aaataatgac tcaatagtaa aaaatacagt
    48721 aggcttagag ctaacaccga ttgaaaaggt gagttcattg ttaacgtata tttatactga
    48781 tgaaagtgac gagactgaga tcattttaac ggattactca aaaattgcta ctgataaaaa
    48841 gtatatattg tggatgtaga cactagtgaa atactttatg atatagaaat tgatgaaaat
    48901 ttactagatg tagcacaaga aatcgatcat caaaagcagg aagctattcg atatgctggt
    48961 agaaaaggga aaagaaaata tttattacgt gaatgtagca aaagttagag aagatgaaaa
    49021 tgagtggaaa gaattcaaga gtcgctactc aattaactcg actccgacat ttacagttta
    49081 cagagaagga tcgatagaaa aaactgtttt ttggactaaa gaaagtggaa tgtctttagc
    49141 tgaagtagaa gagtttttgg attatgttag tatgcaacag taagggagtt cttattatga
    49201 aaaattttga cacatattat gttattgttt tttcgatatc tttgctgtta atattactaa
    49261 ctttaatgca gtataaagag tatccagagt attttatttt aacttttgta tatttagtaa
    49321 gtagctacac acttctatat aaattcagaa gttcttctag agtttctaaa gtttgtttct
    49381 taattagctt attgattcta taatattttt gatttatata ggaaattaaa aatattatag
    49441 aatcaacact cagagaacgg gttggttttt aatagattta ataagtactg ataatgaaat
    49501 ttctcctggt tgggaaaacg aagttggtta ccctgtagaa gaattttttc aagattactt
    49561 tgcggagtat gccgatagga taatgagaga ttacgtaaca gataaaactc aagaagtcat
    49621 caatcaatcc cagtcattaa atgaattatc tgataaggcg aaagaagaag cagttgccat
    49681 tatttcttca gggattagtg actatattga tatgagtggt gaaatcaatt atggcgataa
    49741 agacaaaatt gtacgcatga taaaagcttt tatcaaaact tttgatgtga aggcagatct
    49801 taagtttaat gatacttacc tagtaggaaa attaattaat atccttggga cagagaaaga
    49861 tacggaaacg tttatttcct ctttgtctat gttgtttaca gataagcatt tttcaactca
    49921 gcttggtgga gaggaactag tggaaatctt ttaaccatta cgcccagcat taataaaagt
    49981 atatcaaaaa agtactcctg atgattttta agattggttt gagaaataat gtgaaaacac
    50041 tatttttaat gttaattata gatagagatg aacgcgtcat atggttaaaa gaatgaaaaa
    50101 gtcaaagata cactgctata tttcactaag gatatagcag tgtatctttc atgtcgtttc
    50161 actggcagtt ttttataaaa ggaggtgtgc atttcagttg cttttctttg gtagttagtt
    50221 gaatcgttta agaattaacg gatgtggcag acaggtgctc tgtccaaaaa ccagtaaaat
    50281 ctttgatggt tccatcttta tttagctgtg cctgttcaaa tactgttttg tttttaccga
    50341 atggatcaat tgcaataatt gttccgatcc ccgcacgttt ggcagcaagt aatcctgagt
    50401 aagcatcttc gataacaaga caatcagccg gagctaagct cagtttgtct gccgctttaa
    50461 gaaaaatatc cggttgcggt ttcccaggga aagagccgtc gtcatagaca atcttgtccc
    50521 agtcaaacca gcgggctaaa tcgaaaatat caaaataaaa agcaacattc tctttgacag
    50581 tagccgtggc aatcgttaat ggaatctgtt ggtcagtcag ctggtcaaga gtcgcttcaa
    50641 gcccttttgt caactgaagc tcgttttcat tttgcaagca aagcatacga taatagcttt
    50701 ctttttcttc tgacaatacg gcgatctcag aggcggtcaa atgctctgaa atgaaatgtt
    50761 tcaaaatctc cgtattcgtc cgaccatgaa tattttttaa aatatcctcg tctgataaac
    50821 catcttttgt atgatcatgt atcatatgca gccaagcagc ttcatgcaga tgagagtcca
    50881 aaaacattgt tccgttgaaa tcaaatataa tccctttcat agttacgctc ctatagtggt
    50941 attttcggtt gaatcagcaa tttgcagatc gacatattgt gcatagtgct cttgaacctc
    51001 ccgcgagatc tggcgatccg taatgagcag atcgaaatca tgcaaattat aaaacacata
    51061 aaaatcatct cgattgaatt ttttattatc aacgagtaga tatttcttac gggaattatt
    51121 tagagcgatc tgctgtgctt ctccttcttc ggtactgtaa gtagagatcg cttcgttgtg
    51181 aacgccattt gaactgataa atgcttttgt aaactttagt cttcttaaat tgtcgttggt
    51241 gatcggtccg acaaatgcgc cggtattttt ccggaaatca ccgccaatca gtagaatttc
    51301 tggtccatct gcttgagcaa gaatatcaaa aactggataa ttatttgtaa tgatccgaat
    51361 tttcttgttc agtaaatgcg tcgccaatag ttcgattgtt gtacctggtc ctaagaaaat
    51421 cgtttctccc tcgtgaatca aggaggctgc tgtggcagca atttcttttt tttcttgaat
    51481 ctgcagagtg gctttttcag tgtgagaaag ttcttgatct aaagtgtagg aaagactttg
    51541 agcccctcca tggacacgca gcagcttgcc cgatttttct aattcatcta aatctctgcg
    51601 aacggtcata tcagaaacat ctaattgatt gatgatttca ttaacggtaa ggattccttt
    51661 tttattgacc atttctacga tagtaagcaa tctttctttt ttcaacattg tcttcacctc
    51721 taatttaagg ataatctatc atataaacgg acaacagtca atctaattcg aacaaaagta
    51781 aacatttagg gtggttttta tgtttgttac aatagatttt attgctgttt tctggttttt
    51841 atgattattt gtgtttgttt ttgttgacaa atgttttgga tgcgtttaca atgcaatcag
    51901 ttagaaagag cgaaaactga ttatagattt gaggagagat gtacatgctt aaattaccag
    51961 aagattttat ttttggcgga gccacagcag cctatcaggt tgaaggagcc actaaggaag
    52021 gtggcaaagg cgctgttgct tgggatgact ttttagagga acaagggcga ttcagtcccg
    52081 atccggcaag tgatttttat catcaatatg caaaggacat tgaattgtgt gaacgttttg
    52141 gtgtaaatgg tctaagatta tccattgctt ggagccgcat ttttcctgat ggagctggga
    52201 aaccaaatcc agaaggaatc gctttttatc atcgtgtgtt cgaagaatgt aagaaaagaa
    52261 acgtaacgcc gtttgtgacg ttgcatcatt ttgatacccc taaacgtttg tttgaccatg
    52321 gagatttttt gaatcgagaa acaatagaag catttgtttc ctatgccatt ttttgttttc
    52381 atgaattcaa agaagtcaag gtgtggagta cctttaatga aatctatcca gttgcaacca
    52441 atcaatattt gttaggtgtt ttcccaccag gaatcaaata tgattttaca aagatcgtcg
    52501 cctgtcttca taatatgatg gttgctcatg cccgtgtagt gaactatttc aaagaaaacg
    52561 aacttccggg tgaaattggt gtagttcact cattagagac gaaatatgcg gcaacagacg
    52621 cgccggaaga taagcatgct gcctttttag acgatgcatt gtcgatccgt ttcttattag
    52681 atgctaccta tttaggctat tattcaactg aaaccttgac tgctcttgat gagatatgtg
    52741 aagcaaatca ggccagctat cattttcctg aagaagattt cgttgaattg aaaaaagcat
    52801 ccacgcgtaa tgactactta gggatcaatc actaccaatg ccattttgtc aaagcttacg
    52861 atggagaaaa tgcaatccat cataacggta cgggtgaaaa agggacctct gtttataaag
    52921 tgaagggaat cggagaacgt atttataaag aaggaattcc tcgaactgat tgggactggc
    52981 tgatttatcc agaaggtcta tatgacttgt tgctgcgaat taaaagcgat tacccacatt
    53041 acaacaaaat ctatatcact gagaacggga tggggtataa agatcagttt gaagatggca
    53101 ttattatgga tcagccgaga atcgactatt tgagagtcta tttggaatcc ttaagcaagg
    53161 cgataacagc cggggtaaat gtgaaggggt attttttgtg gtcgcttatg gatcttttct
    53221 catggacaaa tgggtataac aaaaggtatg gcttgtttta tgttgatttt gaaacccaga
    53281 agcgttaccc aaaagaatca gcttactggt acaagttggt cagtgaaacc aaaacgatta
    53341 tttaataagt gataatggag gaagaaaaga tgaataaaga ggaattacaa atgcttggtt
    53401 ttgagatcgt tgcatattca ggcgacgctc gcagtacatt attgaaatta ttgaaagaag
    53461 taagacaagg aaactttgaa catgttgatt cggcgttgaa agaagccgac gaaaatttaa
    53521 cccttgcaca taattcgcaa acaaaaattt tagcggaaga agcatctggg aaagagatgg
    53581 agatgggatt catttttatc catgggcagg atcatttaat gaccacttta ttgctgagag
    53641 atctgattca agactttatt gtactgtaca gacagaatca aggataggag aagaacgcta
    53701 tggatggaat cattaaacaa atcgaaaaag ggaaaccatt ttttgaaaaa atctcacgta
    53761 atagttattt aggagccgtt cgtgacggat ttttaacagc gatgccagct attttatttt
    53821 caagcatttt cattttgatt gctgcggttc ctgagatttt tggttaccaa tggcctgaat
    53881 ctgtctccac atggctatgg aaagtctata attattcgat gggtgttgta gggttactcg
    53941 tttcagcaac cactgctcgc tgcttggctg attcgatgaa tcgtcgctta ccgaaaaaca
    54001 aagtaatcaa caccacctct gtgatgctgg cctctgtggt aagtttcctg ttgttaagcg
    54061 tctctccgat tgaaggtgga ttttcaacgg aatatatggg gacaaaggga ctactggctt
    54121 cctttgtcgc tgcattttta acagttaatt tatataagtt ttgtgtgctg aaagatatta
    54181 ccattaaaat gcctaaagaa gttcctggaa cgatttcgca aaccttccga gatatttttc
    54241 ctttctcttt ttcagtattt gcggccgttt taattgattt aggcattcga caaatcttcc
    54301 aaatctcatt tgcagaagca atcattacgg tgctgcagcc gctatttaca gcagctgacg
    54361 ggtacttagg aatcgctatt atctggggag cgatggcgct gttttggttt gtcggtgttc
    54421 atggaccgtc gatcgtggag ccggcaattg cggcaattat ctatgccaat gtggaaacca
    54481 atctgcaatt gttcaattcc ggtgaacaag cttctaaagt actgacggtg ggtttaggga
    54541 attttgtagg aactatggga ggaactgggg caacattggt tgttcctttt atcttcctac
    54601 tctttgctaa gtctaagcag ttgaaggcag ttgggaaagc ttcctttatt ccagtaagtt
    54661 tcgctgtcaa cgaaccattg ctatttgcgg cacccatgat tttgaaccct tattttttta
    54721 ttccattttt gttaacacct gttgtaaacg tttgcctgtt caagtttttt gtcgatgtat
    54781 tgggcatgaa cagctttatg tacgttttgc cttgggcaac tccagcaccg atcggtttgg
    54841 ttctaggaac gggcatgggt atcttagctt ttgttctagc agcggttcta atcgtcgttg
    54901 acttcctgat ctatctacca ttttgcaaag catacgatgc tgtcttagta gagaaagaag
    54961 cacaaaaagc agaggtgatc gcaaccgagg aaggtgcttc tgccgatgta cgtgaggaag
    55021 tacctgtggc agcgagtcaa gttatcagcg atgcaaataa agaaatcaat gtgttggtac
    55081 tttgtgctgg cgcagggaca agtgcgatgt tagctaatgc tttaaaaaaa ggtgcatctg
    55141 aatcgaaagc accgatcagt gcaatggcgg gtgcttatgg ttctcattac gaaatgctgc
    55201 aggattttga tatggtggtc ttagcaccgc aagtcaattc ttactatgat gatatcaaag
    55261 aagacaccga tcgcttagga atcaagctgg ttgcgacaaa aggagcagaa tatatcggat
    55321 taacgagaga tcctgaaaaa gctgttcagt ttattctgaa ccaatttcaa taaaggagga
    55381 gccataatga aaatgggaat ttggttgcgt aaagctgttt tatttgcagc tgtgttaggg
    55441 gctttggggg ctgtttcaaa aactgttgaa gcatctgcca gcgaatttat tcgcggagca
    55501 gatatttcga tcctgtcaga tatggaaaaa tctggtgcaa aatattatga agacgatatt
    55561 tcaaaagatg ccttagagat tttaaaaaat aatggtgtca actatgttcg actgcgtttg
    55621 tggcaggatc cttataatag taatggagaa agttatggag cagggacaaa tgatctggaa
    55681 acgacgattg accttgcaaa acgagcaaaa aatctgggac ttaaggtgct gctggatttt
    55741 cactacagtg atttttgggt tgatcctggg aaacagaatc ttcctaaagc atggcaaggt
    55801 ctgacctttg aagaaatgaa tacggcattg tacgactata caaagaacgt tctgtcggaa
    55861 atgaagcagc tggacgttta tcctgatatg gttcagatcg gcaatgaact gaatagcgga
    55921 atgctctggc cttatggcaa gtcttgggga gaaggcgggg gagaatttga tcgattggct
    55981 gcttttttga agtctggtat ccaagctgtt cgagacactc aacctaaaac aactccagta
    56041 atgctccact tagctgatgg tggcgataca ggagcattta cttggtggtt tgatgaaatc
    56101 acgagtcgag gtgtgtcatt tgatttaatt ggagtgtcgt attatccgta ttggcacggt
    56161 tcaatgaatg aacttcaaac caatatgaat gcgatcagcc aacggtatgg taaagaagtg
    56221 atcgtagttg aaacagctta tgggcataca acagcgaatg ccgatactat gccaaatgct
    56281 tttggtgaag ccgaagcagc tgcgggggga tatgaaccaa caccgacagg acaagcagag
    56341 tatttgctgg atcttgccga tcggattcag gcagtaccga ataatcgcgg ggcgggcttc
    56401 ttctattggg aacctctttg gtacaacgga aatgtgagtt gggcaacaca agcaggtatg
    56461 aattatctca gtgttcaatc cactatggga aacgaatggg acaaccaagc gatgtttgat
    56521 tttgctggaa acgccttgcc aagcttgcgt gcgtttaaac aagcaggggc gcaaataaat
    56581 ctagttaaaa atgcaagttt cgaaagcgat ggaccaacga ccagtccttc caattggcaa
    56641 acctggtttc aacaaggaac cgctaagggt acgatcaaaa cggaagcggg cacttttttt
    56701 ggggactaca aactaacttt ttggcaggat caagcatacg aggcatcagc ttatcaaaat
    56761 gttagtggca taagcaaagg aacttacaca ttgagtgcct atgtaatgtc tgggggagag
    56821 tttgatacta atcagttata tatcaaaaat tatggtggag cggaactaaa tactgcggtt
    56881 gttaaaacaa gtgaatggac aaaaattaca atcaaagaca ttccaattac aacaggtgtt
    56941 tgtgaaattg gggtttatag caaggcttca ggaaatgctt ggacgaacgt tgattttgtt
    57001 tcattggtga aacaatagac tgcggatcgt ttcgattcaa gatcggaggt tcccttcagc
    57061 cacatcgtta gcgtagaagg caggaatcga acctgcacct cgcctttgcc aaaaataacc
    57121 agaaacgatt tccagtcatt tttaccaaaa cgagtaaagc accagacttc atctacactt
    57181 tgtattatgc taaagagagt tcaacgattt cgtaagaagc aatgaaaaaa ttgcaaaaag
    57241 ttgtgcagat aacataaagt ttgtttaaga aagggtaacc ttagaattcc gtcgtgaata
    57301 tgatttgagc acttagatgg atagaaaaaa gttctcggaa aacagttcgt ttttcgagaa
    57361 cttcttttta tgataagaat gaaacaaatt aacttatatt atagtctctt ttggtaggga
    57421 accatcttat ttttaatgaa aatcaagcat tttagctatt gaagaatgtt gatttatagg
    57481 gattccccct ccatattatg ttggctcttt attcccactc cactgttgct ggtggtttag
    57541 aagtgatgtc atacacgata cggttgacgt gggctacttc gtttacgata cgtacagaga
    57601 ttttttgcaa gacttcccat gggatacggg caaagtcagc ggtcattccg tcgattgaag
    57661 ttacagcacg gataccgact gtgtagtcgt aagtacgtcc gtcacccata acaccgacag
    57721 agcggatccc tggaagaaca gtgaaatatt gccagatatc acggtcaagt ccagcagctg
    57781 cgatttcttc acgcaagata gcatcagatt cgcgtacgat ttctagcttg tcttcagtga
    57841 tttcgccaag aacacgaata ccaagaccag gaccagggaa tggctgacgc caaacgattg
    57901 aatcaggcat gcctaactga gtccctaatt cacggacttc gtctttgaat aatgtgttta
    57961 atggttcgat caattcaaat tgcatatctt ctggcagtcc acctacatta tggtgagatt
    58021 tgatcgtctg tgctgtttct gtaccagatt cgataacgtc agtatacaac gttccttgtg
    58081 ctaggaaaga gacgccttct tcaccagcaa gttttgttgc ttcgtcatca aaaacatata
    58141 cgaattcatt cccaatgatt ttacgttttt gttctgggtc agatacacct ttcaacttgt
    58201 ctaagaagcg atctcttgca ttgactttga tgatgttcaa gccgaatttt ccgcctagac
    58261 tttccattac ttgttccgct tcgcctttgc gtagcagacc gtggtcaaca aagatagatg
    58321 tcaattggtc accgatcgct ttttgtaaaa gaaccccaac gacactagaa tcaacgccac
    58381 ctgaaagacc aagcaagact tttttgtcac caacttgttc acggattttt tctacttgca
    58441 tatcgataaa gttatccatt gtccagttac cttcacagtg acaaacatca aaggcaaagt
    58501 ggcgcagcaa ttcgattccg tattcggaat gacgtacttc tgcatggaat tggataccat
    58561 aaaaattacg tgttgtgtct tggatagaag caatcggaca atctttgctt gttccgactg
    58621 tttcaaagcc ttcaggaact ttagttacca agtcaccatg gctcatccat actgtttgtt
    58681 gttttggtgt ttctttaaac aagacagctt gatcgcttgt tacttctaac aaggctttcc
    58741 catattcgcg gtttttagca ggctctacgg aaccgccaag tttgtacgtg atcaattgca
    58801 taccatagca aatccctaat acaggaatac ctaaattgaa gatctcagga tcgatcccaa
    58861 atgcattctc atcatataca ctgtttggtc cgccagaaag aatgattccc tttggattca
    58921 tcgcttttac ttcttctgca gtaatgcggt ggctcaataa ttcggaaaag actccaagat
    58981 cacgaatacg tcttgtgatc aattggttat actgactacc gtagtctaat acgataatct
    59041 tttcaacgtt cgtcatatcg gcaacgtttg tcaaatttat tacccctatc cctttaaatt
    59101 ttatctcaaa atagtcaaac tattttaaaa tgctaatatt ttcgcatgaa aatcgaatcg
    59161 atgatatgat tttcagtttt atggagaatt atatcggctc ttcctcgtgt aggcaagata
    59221 tattcctgta aatttttcaa attcacattt ttccatacat cttttgccat tttaaaggct
    59281 tcttcacgat ctccgatagc aaaacgataa taataattct ctggattttg gaaagccgta
    59341 tccagtaacg caccaaaacg atccaaatac catttttcga tcagcacggg atcggcatcg
    59401 acatagacag aaaaatcaaa aaagtcgctg acgtagattt gctgattcgc aggtaattga
    59461 agcgtattga tcccctcaac gatcaagata tcaggctgct ggatcaattc gtacttgtct
    59521 ggaatgatat catacacatc atgtgagtaa acaggtactt ttatctcttc ctgaccagat
    59581 tttacacggt tcaagaaatc gatcagcatc tccatatcat aactttcagg aaatccttta
    59641 cggtccatga ttccacgctc ttttaatata cgatttggat aaagaaatcc gtcagtcgtg
    59701 atcagctgaa cgtttcttcg tttgaaggta cgagacaaaa tcgtctgcag cagtctggct
    59761 gttgtacttt ttccaacggc tacacttccg gcgatcccga taataaaagg cggtacttct
    59821 acgtatttat gaagaaataa acctttactc aagaacaaag attcaaattc tttcatataa
    59881 agataaatca gatgagcaag aggtacatag atatctcgaa catcctgcat ggaaatgcgg
    59941 tcattcaaac ttttgatatt atctagttca tcttgtgtca aaggtgcttg cccattacga
    60001 taaaattctc gccattcttc tcttgccaca cggtagtagt tcattggatc attcatatta
    60061 gcctcctgcg ttctcccatc aatggaacta ttttaacaca aaataaacgc aaaccccacc
    60121 acttaagaga gattgccgat ctttttgtat aatctttata atagaaagaa acggagtctt
    60181 attcttgact tgcgcacgga tacataaaag gatagactgt tgtcatgaaa gaaatgagaa
    60241 ggatggaaga catgacaaat cattactatt ctgaacgccc agatacggcg cacgattttg
    60301 aacaatgggc ttttgagtta aaaggcagaa cctttcaatt tgtaacagat tctggtgtat
    60361 tttcacgtga aacagtggat tatggttcac gtgtcctgat tgatgctttc gattgggaag
    60421 agttacctga tgaaggaaag atcttagatg tgggatgcgg gtatggaccg attgggttag
    60481 ctttggcatt tgcgacacag cggtttgttg aaatggtcga cattaatgaa cgagcggtaa
    60541 gtctagcgca aggaaacgca aagcgtaacc aaatcgaaca tgttgatatc catcaatcga
    60601 atatctatga agcagttcat gaagaaacct atgcagctat cgtaagcaat ccgccgatcc
    60661 gtgctggaaa aaaagttgta catgaaattt tgacaggcgc atatcctcgg ttgaagaagg
    60721 gcggtacact gacaattgtg atccagaaga aacaaggagc gccgagtgcg caaaagaaaa
    60781 tggaagaaac atttggtaat gcagaaatcg tcacaaaaga taaaggatat tatatcatca
    60841 aaagtgtgaa agaataacta gctgaaaaat agtggagcga cgaaagagtg gaactgggcg
    60901 tgcatgtacg ggtacgtatc agttcttttt ttgttgagaa gtttttaaaa acaaagaact
    60961 attttttctt caaaaagaaa tagtaggaac agagtcgttg ttttttacga taaaataaat
    61021 aagatacaga acttgtatag aaggctgcca agtcggtaga taaaggaggt ttttatcaaa
    61081 aaacatactt tccttttttc tctattcttt tcttgggggt tttggtacac tgatagggaa
    61141 tttagataga agggagccaa taggatgaag ctgaccatca gagatatcgc agaaatggca
    61201 ggcgtatcag taacaactgt ctcacagatt ttgaataaca aaggaagcag atttagtgat
    61261 aagacaagaa aaaaagtgtt gtcgatcgtg aatgaacatc attataagcc cgattatttc
    61321 gcttcaaatg tgatcaatcg acattcaaag accatcggaa tgatcgtccc tgatgtcact
    61381 gatttcttct tctccaaact gatcgaaggt gtagagagct atttgaatcc tttgggctat
    61441 gtgatcatgt tatgcaattc tcgtcatagc caagaaaatg agatcaagta tttacaagag
    61501 ctttcccacc gctcagtgga cggaatcttg ctggccacac caaatatttt acctgagaaa
    61561 tatgcattag acagcggttt ttatcaaaaa atgccaatca ttttaatcga tcgcggattg
    61621 aatcgtcggg ataacggacg gcttatcgta aaggaatatg agggcgccta tcaagctgtt
    61681 tcgctgttta ttgaaaatgg ccatacgaag atcggcatgc tgaaggaaac cactggatac
    61741 tatcaattag aagaacgttt caacggatat cgtcatgcgc tgaaagatca tggcctgcct
    61801 tttaatggga aatttgttga acatggagag ttgactgtcc aaggtggata tactgcatca
    61861 aaaaaattac tcaaacataa agatatcacg gctatttttt gcgggaatga tgccatggcg
    61921 atcggatgct atcaggcgat cgatgaattg ggcaaaaaaa tcccagaaga tatctcagtc
    61981 ataggattcg atggattaaa gctgtcagaa tacatgattc cgcaattgac gacagtgcag
    62041 cagccaagtt ttgatattgg attttatgct gcacgtttcc tgatcgatac gatcgaattt
    62101 cctcaaagaa aagttccgaa caaagtgttc gaaacaaaat taattattcg tgagagcgta
    62161 aaagtattga caaagggcta actcggcggt atattgctta tgtaagcatt tacaaaggct
    62221 agttagtgct ttttttattt ttagtaaacc agtttactaa agtggtttac ctttcctaaa
    62281 aataatgtat aataccgaat gaagacaagg aggatcaggc aatgcgaatg gtggatctga
    62341 ttgaaaaaaa gagggatgga aacgagctgt cgaaagagga aatcgagtac atcgtcacga
    62401 actatacgaa tggaaagatt ccagattatc aagtcagtgc attattgatg gcaatttttt
    62461 atcaagacat gacaaatgaa gaaatcacga acttaacatt ggcgattgct aattctggtg
    62521 atgtcatcga tttgagttct cttgaaggaa taaaggtaga taagcattcg acaggtggcg
    62581 ttggtgacac gaccacattg attttagccc ccctagtagc tagtgtcgga gtgacagtgg
    62641 ctaaaatgtc tggacgcggt ctaggttaca caggtggtac actagacaag ctagaagcaa
    62701 ttcccggatt tcagatcgag ctttcggatg aagaatttgt acgcattgtt aatgaaagta
    62761 aagtagcggt aattggtcaa tcaggaaact tggcaccggc ggacaaaaaa ttgtatgcct
    62821 tgcgtgacgt cacagcgacc gttgattctc ttccgctgat tgccagttcg attatgagta
    62881 aaaaaatcgc ggcaggagca gatgcaatcg ttttggatgt gaccactgga gacggtgctt
    62941 tcatgaagaa catcgaagat gcaagacgtc tggcaaagac catgacgagc attgggaaac
    63001 tggctaaccg agaaacagtg gcggttatct cagatatgtc agaacctctt ggcgaagcta
    63061 tcggaaatag tctagaagtt gtcgaggcaa tcgaaacatt acaaggaaat ggtccagaag
    63121 atctagtaga aatgtgctac gccttaggaa gccaaatggt tgttcttgct ggcaaagcta
    63181 agacaaccga tgaagcaaga acgttgcttc aagaagctct ggaatcaggt aaagcgttag
    63241 caaaattcaa agaaatgatc caaaatcaag ggggagaccc agcgatcgtg gaacatccag
    63301 aacgaatttt gactgcacgc tacacaatgg agcttcctgc taaacaaagc ggagttgtgt
    63361 caaaaatcgt ggcaaatgaa ttaggaattg ctgcaatgat gctaggtgct gggcgtaaaa
    63421 ccaaagaaga agatattgat cacgctgttg gattgaaact gcataaaaaa attggtgaca
    63481 ctgtgacaaa aggtgagtca ctattgacga tctatagtaa cgacgaagaa atcacttctg
    63541 tcattgaatt attatataaa aatattgaaa ttggtgaatc ggcaatgaag ccaacgttga
    63601 ttcatgacat catcacagaa taaaggaaaa aagcggactt taccgtttta aagaaaaggg
    63661 gaaaagaaat ggaactaaac cgaatgatcg accatactat tttgaaagca gacgcatcta
    63721 aagaagctgt gatgcaaatt atcgaagaag caaaaaaata ccatttttat tctgtctgca
    63781 tcaatccagc atgggttgcg ctagcgaaag aacaattaca aggtgagcca gtagccgtat
    63841 gtacagtaat cgggtttcct ctaggagcaa acacacccga gacaaaagca tttgaaacaa
    63901 cagatgccat caataacggt gcagatgaag tcgatatggt catcaatatc ggtgctttga
    63961 aatcaaagga tgatcaacaa gtccaaaaag atatcgaagc agttgttgaa gcagcaaaag
    64021 atcgtgcgct tgtcaaagtt attattgaaa catcgttatt agatagagaa gaaatcatcc
    64081 gagcttgcga aatagcaaaa gctgcaggcg ctgattttgt caaaacatca actggtttct
    64141 caacaggcgg agcaaaggta gaggatgtcc gtttgatgcg tgaaactgta ggaccagaaa
    64201 tgggtgtcaa agcttccggc ggtatccata acgaagaaga agcaatggca atgatcgaag
    64261 ctggtgctac aagaatcgga acaagcgcag gagtagcaat cgtatctggt tcaactggtg
    64321 aaggctatta attttttcaa aggagaagct catggcagaa ctcaaacaag aatggatcaa
    64381 cacagctatt gaagcgttag ataaagccta tgttccttat tcccattttc cggtaggcgc
    64441 ttgtctcgta acggaatcag gcaagattta tcaaggcatc aacattgaga atgcatcatt
    64501 tggattaacg aattgtgccg agcgcacagc ttttttcaaa gcagtatctg aaggagaaaa
    64561 aacatttacg catctagtcg tagcggggca cacccccgat cctatctctc cttgtggtgc
    64621 atgtcgacaa gtaatggcag aattctgttc ccctgatatg ccggttacgc tagtaggaga
    64681 caatggtgtg acgaaggcaa cgaccgttcg tgcactgctg ccttatgcat ttactgaaaa
    64741 agatctataa ctcatgttca ttcagtcagc tgactgttga aaaataaaac aattatttta
    64801 ggaggcttta cagaaatgaa aaaagcaaaa ttatttggtt taggtgctgt cgcactagca
    64861 gcagggttat ttttaggtgc atgcggcaac aacggctcta ctgattcatc cggtggcaaa
    64921 agctcatcag acacaacaac tgcagcattg atcacagaca caggtggtgt agacgaccgt
    64981 tcattcaacc agtcagcttg ggaaggacta gaaaaatggg ggaaagatca aggactttca
    65041 cgaggaaatg atggcttcca atatttccaa tcttcaaatg aatcagatta catcccaaat
    65101 attgaccaag cattgaatgc tggattcaaa acgatttttg gtattggtta taaactaaaa
    65161 ccagcgatcc aagaacaagc aacaaacaat acaggtacga actttgttat tatcgatgat
    65221 gtcatcgatg gattagataa cgttgtatct gcaactttca aagacaacga agcttcttat
    65281 cttgcaggtg ttgcagcagc ttatacgact gaaacaaacg tagtcggctt tatcggcggt
    65341 gtaaaaggtg aagtaatcga tcgttttgat gctggattca aagccggtgt tgatgcaggt
    65401 gcgaaagaat taggaaaaga aatcaaagta ttgaatcaat atgctggtga cttctctgct
    65461 ccagataaag gacgttcaat tgctcaaggt atgtatgcac aaaacgcaga tatcatcttc
    65521 catgcttcag gcggtacagg taacggtgta ttccaagaag caaaatcatt gaacgaatca
    65581 ggcgacaaaa aagtttgggt tatcggtgtt gaccgcgacc aatcagacga aggcgaatac
    65641 acattaaacg gcgaaaagaa aaacttcaca ttgacatcta cactgaaagc agttggaaca
    65701 gtagtagaag atttggcaca aaaatcagcg gacggtaaat tccctggcgg cgaacacact
    65761 gtttatggat tgaaagaaga cggtgttggt ctaacagaag gacaactttc tgacgaagcg
    65821 aaaaaagcag ttgatgaagc aaaagaaaaa atcatttcag gtgacgtaaa agttccagaa
    65881 actcctgaag aaaactaaaa ccatttctat agccttgtta gacacatgtt tgacaaggct
    65941 atggtttgat tagaacaaga attgttcatt tatccaagct gccttgtgac agtttggata
    66001 agggaacaac tccctgaagt aatgtttgtt tcaaaagaag aacgctcact aagcgtgaat
    66061 cttagtcaat cgttgcccta gatagaaatc gttaggatgt gaaaaaaagg tggttcaaga
    66121 aaatattgtc atagaaatgc agaatattac caaagaattt ggaacattca aggcaaatga
    66181 caacatcaat ttacaagtaa aggcaggaga aattcatgcg ttgcttggag aaaacggtgc
    66241 tggcaaatct acattgatga acgtgctttc cggattatta gagccgacat cagggaaaat
    66301 tttgatgcgt gggaaagaag taaagatcac aagtccgacc aaagccaacc aattagggat
    66361 tgggatggtc catcagcact ttatgcttgt tgatgccttt actgtaacag aaaacatcgt
    66421 gttgggaagc gagcctagtc gcgcagggat gcttgaccat aaaaaagcac gaaaagagat
    66481 ccaaaaagtt tctgaacagt atggattgtc agtcaaccca gatgcttacg ttcgtgatat
    66541 ttcagttgga atggaacaac gggtagaaat tttaaaaaca ctttaccgag gagcagatgt
    66601 actgattttt gatgagccga cagctgtatt gacccctcaa gaaattgatg aattaatcgt
    66661 gatcatgaag gaattagtca aagaaggcaa gtcgatcatt ttgattacgc ataagttaga
    66721 tgaaatcaaa gcagtagctg accgctgtac agttatccgc cgtggaaaag gaatcggtac
    66781 agtcaacgtt aaagacgtaa cctcacaaca attagccgat atgatggttg gaagaacggt
    66841 ttcattcaaa acgatgaaaa aagaagcgaa gcctcaagaa gtcgttttgt ctattgaaaa
    66901 tctagtggtg aaagaaaatc gtggattaga agccgtgaaa aacttgaact tagaggttcg
    66961 tgctggcgaa gtacttggta tcgctggaat cgatggaaac gggcagtcgg agttgatcca
    67021 agctttgact ggtttgcgaa aggcagaaag cggacatatc aagctaaaag gggaagacat
    67081 cactaataaa aaaccccgaa aaatcactga acatggtgta ggacacgtgc cagaagaccg
    67141 tcataaatac gggttagtcc tagatatgac attatctgaa aatatcgcac tacaaaccta
    67201 tcatcaaaaa ccttacagta aaaacggcat gctgaattat tcagtaatga atgaacatgc
    67261 cagagaattg atcgaagaat atgatgttcg aacaacgaat gaacttgttc ccgcaaaagc
    67321 tttatcaggc ggaaatcagc aaaaagcaat tatcgctcgt gaagtcgacc gagatcctga
    67381 tctgttgatt gttgcaaatc caactcgtgg actggatgta ggtgcgatcg aatttattca
    67441 taaacgtctg atcgaacaaa gggacaaata caaagcagtg ttactgatca gtttcgaatt
    67501 agaagaaatt ttaaatgttt cggatcgtat tgctgttatc catgaaggag aaattgtcgg
    67561 tatcgttgat ccgaaagaaa catctgaaaa tgaattaggt ctattgatgg cgggctatac
    67621 cttagaagaa gcacgaaaag agttagaaaa gggggctggc gcacatgaat gatcgcagcg
    67681 aaaaattccg taatttatta gtgcctgtac tttcagttgt tttaggattg atcttaggcg
    67741 cgatattgat ggctgctttt ggttttcagc ccatccaagg ttattcttct atgctagatg
    67801 cagcccttgg ttcgcaaaga gggataggtg aaacgttaag gcaagcaacg ccgttgattt
    67861 ttacagcatt aggtttttct gtagctaact cagctgggtt tttcaatatt gggctttctg
    67921 gacaagcact ttgcgggtgg atcgtcagtg tctgggtggc acttgcgttt ccagatctgc
    67981 caaaagttgt tttgcttccg ttatgcgtga tcttaggtgc cttagctggt gcgatggcag
    68041 cggctgttcc aggtttactt cgagcatttt ttggaacaag tgaagtgatc gtaacaatca
    68101 tgatgaacta tatcttatta tacgtaagca catttgcttt acatgaagtg atgcctgaaa
    68161 gctatcgctc aagtttggac tcttccaata tgatcagtca aaatgcctca ctgcggttac
    68221 catggttaac acaagctttt ggcggctctc gcgtaaactc aggattattt ttagcaatca
    68281 tcgcgcttat tttcgtttgg ctgctgatga agaaaacgac tttaggattt gaaatccgtt
    68341 ctgttggatt gaatcctttt gcttctgagt atgcaggaat gagtagtaaa cgtacgatcg
    68401 ttttgtcgat ggtcatttct ggcgctttgg cagggcttgg cggtgtggtt gaaggattag
    68461 gaacgtatca aaacttcttt gttcaaacga cttcgctatc tattggattt gacggaatgg
    68521 ctgtttcctt gcttggttct ggatcagcaa tcggtattct tttatcagcg atcttgttca
    68581 gtattttgaa gatcggtgga cttggcatgc agacaggagc aggtgttccg tttgagatcg
    68641 tcaatgtatc gattgctttg attatcttct ttgtcgggat caacttcctg attcgtttcg
    68701 tcatggctaa agtcttccaa gggaaaaaac aagaagagat cgttgcgaca atagaatcga
    68761 aaccaacgaa tcaaaaccat gaagggggcg aactataatg tcgactatcg aattaattgc
    68821 cttgatcctt acttctacac tggtttattc aacaccgcta attctaacgt cactaggcgg
    68881 aactttttct gaacgaagcg ggatcgtaaa cgtagggcta gagggaatca tggtcatggg
    68941 tgcttttagt tccatcgttt tcaacttgac gatgagtcca tcacttggtt ctatgacacc
    69001 atggatcgct attttagtgg gtggcgtcgt aggtatccta ttttctctga ttcacgcagt
    69061 agcaacagtc agtttgcgtg ctgaccatat tatcagcggg acagtaatta atctgatggc
    69121 acctgcttta ggtgttttct tgatcaaagc atggtatggt aaaggtcaaa ctgataacat
    69181 cacgcagaat ctggggtatt tttctttccc aggattagca gatattccgg tcattggacc
    69241 aattttcttt aaaaatacat tcttacctgc ttattgtgct attttgattg ctgtcctagc
    69301 ttgggtgatc atcttcaaga cgaaattcgg cttgcgcctc cgttcagttg gtgaaaatcc
    69361 tcaagctgcc gacacattag gtatcaatgt atatgctatg cgttattcag gcgtactgat
    69421 ttctggcttg ttaggcggta tgggcggtgc tgtttttgct cagtcgattt ccggtaactt
    69481 ttcagctggt acgatcgttg gacaagggtt catctccatg gctgctatga tctttggtaa
    69541 gtggaatcct ttaggggcaa tgggtgcctc attgttcttt ggttttgctc aaagcttgag
    69601 tatcattggc gcacaattac cagttatttc ttcgattccg ccagtattgc tgcaaattgc
    69661 tccatatttg ctgacaatca ttgtgctggt tgttttctta gggaaagctt ctggtccaaa
    69721 agcaaacggg aagaactata tcaaatcaaa ataatctttt agcgaataaa aagggtgggt
    69781 tacgggaagt gtttttcgtc tcgccctttt tgttttaaag gcaataaaaa taaaaagaaa
    69841 gaaggaataa tcatgtttaa acgcgtacat ctgatcgtca tggattcagt aggaattgga
    69901 gaagcaccag atgccgataa gttcggtgat gttggttctg atacattagg acacattgcg
    69961 aaagaagcag gattgaacgt tccacattta gagcagcttg gtttaggaac gatccgtcct
    70021 cttgaaggtg tggaatcagt gaaagatcat caaggctatg cgacgaaatt agaagaagtt
    70081 tctgtaggga aagatacgat gacaggccac tgggaaatca tgggattgaa tatcaaaact
    70141 ccatttcgtg tgtttcctaa tggattccct gatgatctga tcaaacaaat cgaagaattt
    70201 tctggccgta aagtcgtatg taacaaacct tatagcggaa cagctgttat tgacgattac
    70261 ggtgaacacc aaatgaaaac aggcgattta attgtatata cttctgccga tcctgtcctt
    70321 cagatcgcgg cacatgaaga aatcattcct ttagaagaat tatatcggat ttgccaattc
    70381 gttcgtgata taacaaaaga tgatccgtat atgattggcc gaatcattgc acgtccgtac
    70441 gtgggtgaac caggagactt cacacgaaca agcaatcgcc atgattatgc gttgaatcca
    70501 ttcggacaca cagtccttga ttctttgaaa gaagcaggaa aggacgtgat tgctgttgga
    70561 aagatcaacg atattttcaa cggacaagga atcactgaat ctgtccgcac gaaaagcaat
    70621 atggatggcg ttgaccagct gctgaaagtt atgaaaaacg atttttcagg aatcagcttt
    70681 accaatttag tagattttga tgcattatac ggtcatcgtc gggatgtagt cggctatgcg
    70741 catgcgattg aagaatttga cttacgaatt ccagaattgc ttgaagcgat ggaagaagac
    70801 gacttgctaa tgattacagc cgatcacgga aacgatccga cgtttactgg taccgatcat
    70861 acgcgagaat atgtaccgtt attggtttac agtaaaaaaa tgaaagaaca aggggcattg
    70921 cctcaaggat attattcaga tatttcagca acgattgctg agaactttag tgtttcttcg
    70981 actgaaaatg gcgaaagctt cttgaaatta ttaaaataat ctttttaaat aggagaaagc
    71041 aaatgaccaa gttaagcgag atgttacaag aaacgaccca gtttatcctt gataaaggag
    71101 taaaagaaat tgattttggt ttgattctag gttcaggatt aggtgaatta gcagatgaaa
    71161 tcgaagatgc tgtaatgatt tcttatgatg agatcccgtt ctttcctgtt tcgacagtag
    71221 ttgggcatgc aggaaagcta gtttatgggt ctctggcagg caagaaagtg ttagccatgc
    71281 aagggagatt ccactactat gaaggtcatt ccatgcaaac agtgacctat ccagtacgtg
    71341 tgatggccgg actaggggcc cattcagtca ttgtgactaa tgcatgtggt ggtgtcaacg
    71401 aatcatttgt accaggagat ttgatgctga ttaccgatca tatcaatttt actggtgaaa
    71461 atcctttgat cggtccgaat gaagatgcaa tgggccctag atttccagat atgagtcaag
    71521 cttatacgcc tgactatcaa gcaacagcaa aagcagcagc agaaaaacta ggtttggatc
    71581 ttaaagaagg cgtatatatg ggctattctg gaccaactta tgaaacgcct gcggagatcc
    71641 gaatgtctcg gacaatagga gccgatgcag tagggatgtc gactgttcca gaagtcatcg
    71701 tagcagctca cagcggatta aaagtgttag gaatctcttg tattaccaac ttagcagcag
    71761 gtatgcaagc gaatctgagc catgaagaag ttgtagaaac aactcagaga gtcaaacaat
    71821 cttttaaagc attggttaaa gaaacattga cattattgta ataggaggaa caataaaatg
    71881 agtgtgcata ttgaagcgaa agaaggcgag atcgccgata agattttact tccgggagat
    71941 cctttgcgag ctaaatatat tgcggagact tttttggaag atccagtttg ttacaatcag
    72001 gtacgcggta tgttaggctt taccggcaaa tataaaggag aacgtatttc tgttcaagga
    72061 acaggaatgg gaatgccatc agccaccatc tatgcccatg aattgatcca gtcgtatggt
    72121 gtaaagaaat tgattcgagt aggtacatgc ggtgcattgt caaaagacgt ccatgttcgt
    72181 gatttagtac tagcacaagg tgcagcgacc agttcttcga tgatcgaaaa aaatttccaa
    72241 gcctttcatt ttcctccaat cagtgatttc aatctattac tgaaagcata tgagatagct
    72301 aaagaaaagg gctatacggt tcatgtagga aatgttcttt cggaagattc tttctacaaa
    72361 gatgatttga cagagacgtt ccaattagct gaacttggcg ttttaggagt ggaaatggaa
    72421 gcagcggctc tttattactt aggcgcgaaa taccatgtgc agacattaag tttgatgacc
    72481 gttagcgacc atttgatcac cggtgaagaa acaacagctg cagaacgtca atctacgttc
    72541 aatgaaatga tcgaagtagg attggaaaca gcgatagcag aataagaagg aaatgaaaaa
    72601 gctgccctgc attaagtaat aagaacatcc aagcaaaaat agctcctcat gtttttgact
    72661 tggctggact tattattgca aatgtaagct tttgaacacc gtttaccaag aggttgtgaa
    72721 tctgccgttt aactctgagt cacaaggaac aatttcaaaa aacagctttt catgtttgat
    72781 gaatattgtg acttgtgacc gagaagttgg caggagaaaa ccgtttatac gaaaaaaggg
    72841 actgtgacaa atcgtgtcac agtccctttt ttatagtgtt ttttataggt agtatttttt
    72901 agcaactgtt aagtcatcat ttaattcata aacaagtggt tgacctgttg gaatttcaag
    72961 atccatgatg tcttcgtcag agataccttc gatatgttta gctagggcgc gtaatgagtt
    73021 accatgtgca gctactaaaa cagttttgtt gtctagtaat gctggagcaa tttcatcttg
    73081 ccagaatggc aaagcgcgtt ctaatgtcac tttcaagttt tctccaccag gaatatcgcg
    73141 tttgtctaac attgcgtaac gacggtcatt tgctgcagaa ccttcgtcag tttcttccat
    73201 taatggagga agagtatcgt aagaacgacg ccagatgtgc acttgttcgt cgccgtattt
    73261 ttcagctgtt tcttttttgt tcaaaccttg taatttacca tagtgacgtt cgtttaagcg
    73321 ccatgatttg atttgtggta cccaaagttg atctgaatat tctaagatca agttacatgt
    73381 tttgattgca cgagttaaaa cagaagtgta agcaacatca aattcgattc cagcttcttt
    73441 gattttgcgt ccgccttctt ttgcttcttc gattccttct ggtgctagat ttacatcagc
    73501 ccaaccagta aattggttca atgcattcca ttcactaaga ccgtgacgag aaaaaactaa
    73561 ttttggcatg tttagctctc cttttaagat aggtttatat ttctgaaatt ttcagttctg
    73621 ttttattgta caattttttg gaagagattt ccatcgaaaa attagataag attctatgag
    73681 aaaacaagta gagaatataa aaaaacgaac agtgcccacg caactgttcg tttttaggta
    73741 ttagatgcat aattgctcga tttctttttc aggtaaattg atatatttat aacatgttcc
    73801 aactgaaaca ccgcattttg aagaaataaa ttggatcgtt tctttttttt cataatagag
    73861 acgtcggatt tttttgatcg tttttgcatc gatttttggt cttccgccga tttttccttt
    73921 tttacgagct tcatctaatc caactaatgt acgttctttg atcaatgcgc actccatgtt
    73981 tgctaggttt tccattaatt ggaaatagat ttttcccatt tcgcgcgtat cgattccttc
    74041 atcaagacta atcaagtgca gcccgcgatc tttgaataat tgcgtgagtt ctgttaattg
    74101 ccgcgtagat ttgcctaatc gatgaagttg gcaaatgact agtgcatcgc cttgttgcat
    74161 gtcagcgatc actgactcca aatgaacagc agtagaatca ttttgttcca cttcatatgt
    74221 ctcatggaaa atttcttgac aaccaaagtc agataattga tcaacctgag ttgttaaatc
    74281 aacgtcattt atggttgtgc gtgcataacc aataattttc atggtgattc ctttctagca
    74341 gtgattttct aactggatat aacagtaaaa atgtagcata aagtacgtca aaaagatagt
    74401 tttttcgcaa attttccgct gtcttttttt attggatgat caagcagttt ttctttattt
    74461 acgacggatt gcgtcaagaa tctcaacgcc tttttctgag ccgacaacta ctttagaaac
    74521 catgtttagg aaaagcccat gttctactac cccaacaagt tcatctaaat aggtagctaa
    74581 gctttctggc tgttcgatga cttctagatg cagatcaatg atgtaatgac caccatctgt
    74641 ggttaagact tctccgtttt cgtctgtacg caagacgggt tggaatcttt tttcttcaaa
    74701 aatatgaagt agctgttggg agccataagg gatcacttca actgggagtg ggaacttccc
    74761 aagtttatcg actaatttag aagaatcaac gatccaaatc gtttcttttg agtacgtagc
    74821 gactattttc tcgaaaagca aagcagcgcc gccgcctttg atcccttgaa aatcagcact
    74881 gatttcatca gcaccgtcta ttgtcaaatc gactacgggg acttcatcaa tactctttaa
    74941 cggtattcct aatttttccg cttgttcttt tgtcgcattt gaagtagtaa ctcctgtgat
    75001 acgtaaacct tcttcacgca tacggcgacc aatctcttct accatataat aggcggttga
    75061 accggtcccg agacctacga tcatcccgtc ctttacgaat ttcgcggctt caatgccagc
    75121 taattgtttt aaattcatct ttgcgtccac ctttttttag tttgagcatc gtgctttcct
    75181 cactatttta gcgtgattta cggtaaaaag ataccattct tttttattcc aataaagctt
    75241 tttcaaaaaa aattgcatct cttgttgaca tgttttttca ttcgtgatat tctaacaaag
    75301 gtgctttatc tacaggtttc tgtgaagaaa cgtgctgact gtttatcaaa gcatatttga
    75361 taaggatgca gacattgcat ttatttttta tcacaaaaaa agaaccacct ggatgtgtgg
    75421 aactagacgc aaatcgtggg aaggaggaaa caaggaatgc ctacaattaa ccaattagta
    75481 cgtaaacctc gtaaatcaaa ggtggaaaaa tctaattcac ctgctttgaa caaaggatat
    75541 aacagcttta agaaaactca aactaacgta aactcacctc aaaaacgtgg ggtagcaact
    75601 cgtgtgggta caatgacacc taaaaaaccg aactcagctt tacgtaaata tgcacgtgtt
    75661 cgtttgtcaa acttaattga agttactgct tatatcccag ggattggtca taacttacaa
    75721 gaacacagtg ttgtattatt acgcggtggt cgtgtaagag acttaccagg ggtacgttac
    75781 catatcgttc gcggtgcttt agatactgct ggtgttaacg accgtaaaca aagccgttct
    75841 aaatacggaa ctaaaaaacc taaagcttaa ttttaattat taaacaaatg aaacttgctt
    75901 ctactatata gaagtaatta ctatgaacaa tatttcggaa ggaggagtta cggatgccac
    75961 gtaaaggtcc tgttgcaaaa cgtgatgttt taccagatcc tatttataac tcaaaattag
    76021 taactcgctt gatcaaccgt gtaatggttg acggaaaacg cgggattgca gctaacatta
    76081 tctataatgc ctttgacttg atcaaagaat ctacaggtaa cgatccatta gaagtgtttg
    76141 aacaagcaat gaaaaacgtt caacctgttc ttgaagttaa agcacgtcgt gttgggggtt
    76201 caaactacca agtaccagtt gaagttcgcc ctgaacgccg cgttactcta ggtcttcgtt
    76261 gggtagtaaa ctacgctcgc ttgcgcggtg aacacacaat ggaagaacgc ttagcaaaag
    76321 aaatcatgga tgctgcaaac aacacaggtg cttctgttaa aaaacgtgaa gacactcata
    76381 aaatggctga tgccaaccgt gcgtttgctc actatcgctg gtaagattct ctctgtttgg
    76441 tcggctttta taagtcgacc tcagatagtg tccttatttt tagcaactag atagtaaaga
    76501 gaggagaaac aaggagaatg accagagaat tttctttaca aaacactcgt aatatcggta
    76561 tcatggccca catcgatgct ggtaaaacga ctgctactga acgtatcttg tactatactg
    76621 gtaaaatcca taaaattggt gaaactcatg aaggagcttc acaaatggac tggatggaac
    76681 aagaacaaga acgtggtatt acgatcactt ctgctgctac tacagcacaa tggaagggtc
    76741 accgaatcaa catcatcgac acaccaggac acgtagactt cacagttgaa gttgaacgtt
    76801 cattgcgtgt acttgatggt gctgtaactg ttcttgatgc acaatcaggt gttgaacctc
    76861 aaactgaaac agtttggcgt caagcaacaa cttatggtgt acctcgtatc gtatttgcaa
    76921 acaaaatgga caagatcggt gctgacttct tatattcagt atcaacttta catgaccgtt
    76981 tgcaagcaaa cgctcaccca atccaattac caattggtgc ggaagacgac ttcacaggaa
    77041 ttatcgacct tgtaacaatg aaggctgaaa tgtatacaaa cgatcttgga acagagatcg
    77101 aagaaacaga aattcctgaa gaataccgcg aattagctga agaatggcgc gaaaaattgg
    77161 ttgaagctgt agctgaaact gatgaagaac ttactttgaa atacttagaa ggtgaagaaa
    77221 tcactgaagc tgagttaaaa gaaggtatcc gccgcgcaac agttaacgta gaattctacc
    77281 cagtactatg tggttcagca tttaagaaca aaggggttca attactattg gacgctgttc
    77341 ttgattactt gccatcaccg ttagatatcc cagctatcaa agggattgac cctaaaactg
    77401 atgaagaagt agaacgtcct gcagacgaca gcgctccttt ctcagcttta gcatttaaag
    77461 ttatgactga cccattcgta ggtcgtttaa ctttcttccg tgtgtactct ggtgttttac
    77521 aatcaggttc atacgtacaa aacgcaacaa aaggcaaacg tgaacgtgtt ggtcgtatcc
    77581 tacagatgca tgccaactct cgttcagaaa tctctgaagt ttacgctgga gatatcgctg
    77641 ctgctgttgg tttgaaagac acaactactg gggacacact atgtgatgaa aagagtctag
    77701 ttattcttga atccatggaa ttcccagaac cagttatcca agtcgctatt gaacctaaat
    77761 caaaagctga ccaagataaa atgggtgtgg ctttacaaaa actatctgaa gaagatccta
    77821 ctttccgtgc tgaaacaaac gtggaaacag gcgaaacaat catcgctggt atgggtgaac
    77881 ttcacttaga tatcatcgtt gaccgtatgc gtcgtgaatt caaagttgaa gctaacgtag
    77941 gtgcgcctca agtatcttac cgtgaaactt tccgtgctgg cactcaagcc gaaggtaagt
    78001 ttgtacgtca gtctggtggt aaaggtcaat acggtcacgt atggattgaa tttacaccaa
    78061 atgaagaagg tgctggtttc gaatttgaaa atgcgatcgt cggtggggtt gttcctcgtg
    78121 aatacattcc agctgttgaa actggtttga aagatgctat ggaaaatggt gtcttagctg
    78181 gctatccatt agtagatatc aaagctaaac tttacgatgg ttcataccat gatgtcgatt
    78241 caaatgaaac tgccttccgt gttgctgctt ctatggcatt acgtgcagca gctaagaaag
    78301 ctaatccagt aatccttgaa ccaatcatgg ctgtagaagt tgttatccct gaagattatt
    78361 taggtgatgt tatgggtcac gtaactgctc gtcgtggacg tgttgaaggt atggaagctc
    78421 gcgctggcgg ccaacaagtt gttcgtgcga tggttccact tgctgaaatg tttggttatg
    78481 ctacaactct tcgttctgct actcaaggtc gtggtacatt tacaatgaca tttgaccact
    78541 acgaagatgt accaaaatca gtacaagaag aaatcattaa gaaaaacggc ggaaaagctg
    78601 aataatttct tgaaaccgga tagtcctgca aagagattat ccaaagagac tatattttta
    78661 atatagtttc gtgtaaaata attatcgatg atatgggcat gtggtgtacc ttgtgtacat
    78721 catataccta tcagacaaat aaaaaatata ttgtattttt aggaggacat tttaaaatgg
    78781 caaaagaaaa atttgaccgt tctaaaccac acgttaacat tggtactatc ggacacgttg
    78841 accatggtaa aactacatta acagctgcaa tcacaactgt actatctaag aaaaacggcg
    78901 gccaagctat ggcttacgat caaatcgatg gtgctccaga agaacgcgaa cgcggaatca
    78961 caatctcaac agctcacgtt gagtatgaaa cagatacacg ccactacgct cacgttgact
    79021 gcccaggaca cgcggattac gttaaaaaca tgatcactgg tgctgctcaa atggacggag
    79081 ctatcttggt agtttctgct gctgacggcc caatgcctca aactcgtgaa cacatcctat
    79141 tgtctcgtca agttggtgtt ccttacatcg ttgtattctt gaacaaagta gacatggttg
    79201 atgacgaaga attactagaa ttagttgaaa tggaagttcg tgacctatta acagaatacg
    79261 aattccctgg tgacgatgtt cctgtagttg ctggatcagc tttgaaagct ctagaaggcg
    79321 acgcttcata cgaagaaaaa attcttgaat taatggctgc agttgacgaa tacatcccaa
    79381 ctccagaacg tgacaacgac aaaccattca tgatgccagt tgaagacgtg ttctcaatta
    79441 ctggacgtgg tactgttgct acaggtcgtg ttgaacgtgg acaagttcgc gttggtgacg
    79501 aagttgaagt tgttggtatt gctgaagaaa cttcaaaaac aacagttact ggtgttgaaa
    79561 tgttccgtaa attgttagac tacgctgaag ctggagacaa cattggtgct ttactacgtg
    79621 gtgttgcacg tgaagacatc caacgtggac aagttttagc taaaccaggt acaatcacac
    79681 ctcatacaaa attctctgca gaagtatacg tgttgacaaa agaagaaggt ggacgtcata
    79741 ctccattctt cactaactac cgtccacaat tctacttccg tacaactgac gtaacaggtg
    79801 ttgttgaatt accagaagga actgaaatgg ttatgcctgg cgacaacgta acaatggaag
    79861 ttgaattaat ccacccaatc gctatcgaaa acggtactaa attctcaatc cgtgaaggcg
    79921 gacgtacagt tggtgccggt gtcgttacag aaatcaaagc ttaattcact taagttttaa
    79981 agtaaagcaa catttattcg gacgtaatcg ctcggaacga aggtcttagg atctttgttc
    80041 cgagcttttt tatttattgc aggatcagga aaaaagacag gagtattata aatagaagaa
    80101 acacgaaata aaagagtcag tattttctaa atgaaacttt atgaaaacaa aatgtttttt
    80161 acaagaaaaa gccttgcttt gtgagtttat atatattata atgacaaagg tgcgtttact
    80221 ttagtgaatt gcacagacgt aatcagaatg atttcgtcgg ctaagaaacg agaggttgcg
    80281 acacgcccgg acgctttgcc acgaacgagc gtgacggaaa ttttcgtgga gctatgtcta
    80341 cttttcaaaa taggcgaagg agggaacaag atggcaaaac aaaaaattcg tatccgttta
    80401 aaagcgtatg aacaccgtat tttagatcaa tcagcggata aaatcgtgga aactgcaaaa
    80461 agaactggag ctagcgtgtc tggtccaatt ccattaccaa ctgaacgcag tctttacaca
    80521 atcatccgtg cgactcataa atacaaagat tcacgcgaac aattcgaaat gcgtacacac
    80581 aaacgtctta tcgacattgt gaacccaaca ccaaaaactg ttgatgcttt gatgaagcta
    80641 gacttaccat ctggtgtaaa catcgaaatc aaattataaa actaaaacaa aaataatgga
    80701 ggtgtactca tgaccaaagg aatcttaggg aaaaaagtgg gaatgacaca aatcttcact
    80761 gaatctggtg aattaattcc agtaactgtt gtagaagcta ctccaaacgt agtactacaa
    80821 gtgaaaacaa tggaaacgga tggctacgaa gctattcaag ttggttacca agatatgcgt
    80881 gaagttttat caaacaaacc tgcgaaaggt catgttgcaa aagcaaacac ggctcctaag
    80941 cgcttcattc gtgaattcaa aaatgttgag ctaggagaat atgaagtagg aaaagaaatt
    81001 acagtagatg ttttccaagc aggagacatt attgatgtaa caggtactac gaaaggtaaa
    81061 ggattccaag gggttatcaa acgccacgga caaagccgcg gaccaatggc tcacggttct
    81121 cgttaccatc gtcgtcctgg gtcaatgggt ccagttgcac ctaaccgtgt atttaaaaac
    81181 aaacgtcttg ctggccgtat gggtggcaac cgcgtaacta tccaaaactt ggaagttgta
    81241 cgtgtcgatg cagaaagaaa tgttatcttg atcaaaggta atgttcctgg agctaaaaaa
    81301 tctttaatta ctatcaaatc agctgtgaaa gctaaataat tgtaggaaga aaggaggaac
    81361 taaggaatgc cgaatgtagc attattcaaa caagatggaa gccaaaacgg tgaaatcaca
    81421 cttaacgaag aaatcttcgg aattgaacct aatgaaagtg ttgtttacga tgcaatcgta
    81481 atgcaacgtg cgtcattaag acaaggaaca cacgcggtta aaaaccgtag cgctgttcgc
    81541 ggcggcggac gtaaaccatg gcgtcaaaaa ggaacaggtc gtgcacgtca aggatctatc
    81601 cgctcaccac aatggcgtgg aggtggcgta gtctttggac caacaccacg ttcttacagc
    81661 tacaaacttc ctaaaaaagt tcgtcgttta gcaatgaaat ctgtattgtc agaaaaagtt
    81721 gctgaaaaca acttggtagc aatcgaaggc ttgaacttcg acgcaccaaa aacaaaagaa
    81781 ttcaaacaag ttcttgctaa cttatctatc gactctaaag tattggttgt tctagaaaaa
    81841 ggtaacgact ttgctgcttt atcagcacgt aacttaccaa acgtttctgt agtaacttct
    81901 gataatgtga gtgtgctaga cgttgtatca aatacaaaag tgttagcaac acaaactgct
    81961 cttactcaaa ttgaggaggt gcttgcataa tgaacttact agacgtaatc aaacgcccag
    82021 tgatcactga aaaatcaatg cttgccatgg atgacaagaa atacactttt gaagtggaca
    82081 ctcgcgcaaa caaaacttta gtaaaacaag cggtagaagc tgcttttgac gttaaagtca
    82141 aaaacgtgaa catcgtgaac gtacgtgcga aattcaaacg tatgggcaaa tatgcaggat
    82201 atacaaaaaa acgtcgcaaa gcgatcgtta cattgactga agattcaaaa gaaatccaat
    82261 tattcgaagc tgctgaataa gcactaatag ataactcatt aaattaggag ggaaaaacgt
    82321 ggcgattaaa aagtacaaac ctacctcaaa tggtcgtcgt aacatgacag gttctgattt
    82381 tgctgaaatc acaacatcaa cacctgaaaa atcattgtta caaccattga aaaaccatgc
    82441 tggacgtaac aacaacggcc gcatcactgt acgtcatcaa ggtggcggac acaaacgcca
    82501 atatcgcgtg atcgacttca aacgtaacaa agataacgtt gttgcaactg ttcaaacgat
    82561 cgaatatgat ccaaaccgtt cagctaacat cgcgttagta cactatgaag atggagtgaa
    82621 agcttacatc ttagcaccta aaggactaga agtgggtatg aaacttgttt caggtccaga
    82681 agcagatatc aaagtcggaa atgctcttcc attagaaaac attccagttg gtactgttat
    82741 ccacaacatt gaaatgaaac ctggtaaagg tggacaattg atccgttctg ctggtacaag
    82801 tgctcaagta cttggtaaag aaggcaaata cgtattgatc cgcttgaact ctggtgaagt
    82861 tcgtatgatc ttagctgcat gccgtgctac aatcggttct gtaggtaacg aacaacacga
    82921 attaatcaac atcggtaaag ctggacgctc tcgttggatg cgtaaacgcc caactgtacg
    82981 tggtagcgta atgaacccta atgatcaccc acacggtggt ggtgaaggta aagctcctat
    83041 cggacgtaaa gcaccagttt ctccttgggg tcaaccagct cttggttaca aaactcgtaa
    83101 caaaaaagct aaatcagaca aacttattgt tcgtcgtcgc aaaaataaat aattactttg
    83161 accatgtgtc gcacattttg agaggaggtt caccatgggt cgtagtttaa agaaaggacc
    83221 ttttgtcgat gagcatttaa tgaaaaaagt tgaagctcaa gcaggggttg aaaagaaaaa
    83281 agttatcaaa acttggtcac gccgttcaac agttttccca agttttgttg gatataccat
    83341 cgcagtttat gatggacgta aacatgtacc agtttacatc caagaagaca tggtaggaca
    83401 caaattaggt gaatttgcac ctacaagaac ttatcgtgga catgctgctg acgataagaa
    83461 aacgaaacgc taatttgagg ggaggataaa tccaaatggc agaacaaatc acatcagcta
    83521 aagcaactgc aaaaacagtt cgcacttcac ctcgaaaagc ccgtttagta attgacctta
    83581 tcagaggtaa aagtgtcgca gatgcaattt caatcttgaa attcacacca aacaaatctg
    83641 ctggaatcat tgaaaaagta ttgatgtcag cagttgcgaa tgcagaaaat aactttgact
    83701 tagatgttga aaacttggta gtaagcgaag cttttgttaa cgaaggacct acaatgaaac
    83761 gtttccgtcc tcgtgcaaaa ggatcagctt ctccaatcaa caaacgtaca agtcatatca
    83821 cggtagtcgt atcagaaaaa taaggaggga taatcagtgg gtcaaaaagt acatccaatt
    83881 ggaatgcgtg taggcatcat ccgcgactgg gatgctaaat ggtatgctga aaaagagtat
    83941 gctgagttct tacacgaaga tttaagaatc cgtaaattta tttcaactaa acttgctgat
    84001 gctgctgtat caacagttga aatcgaacgc gcaacaggtc gcgtaaacat ttcaattcac
    84061 acagctaaac caggtatggt tattggtaaa ggcggttctg aagtcgaaag cctaagaaaa
    84121 gaattaaaca aattaactgg taaaagaatc catatcaata tcgtggaaat caaaaaacca
    84181 gatctagatg caaaattagt aggtgaagga atcgcacgtc aattagaaaa ccgtgttgct
    84241 ttccgtcgcg ctcaaaaaca agccatccaa cgctcaatgc gtgctggcgc tcaagggatc
    84301 aaaactcaag tatctggtcg tctaaacgga gcagatatcg ctcgttcaga aggatactca
    84361 gaaggaactg ttcctcttca tactttacgt gcggacattg attacgcatg ggaagaagca
    84421 gatacaacat acggaaaact aggagttaaa gtgtggatct atcgtggaga aattcttcca
    84481 actaaaaaaa acactgagaa aggagggaaa taatcatgtt agtacctaaa cgtgtaaaac
    84541 accgtcgtga attccgcgga aaaatgcgcg gggaagctaa aggcggaaaa gaagtagcat
    84601 tcggtgaata cggtttgcaa gctgttgatt cacattggat cacaaaccgc caaatcgaag
    84661 ctgctcgtat cgcaatgact cgttacatga aacgtggtgg gaaagtatgg attaaaattt
    84721 tccctcacaa atcttatact gccaaagcaa ttggggtacg tatgggttct ggtaaagggg
    84781 cacctgaagg atgggttgca ccagtaaaac gtggtaaaat catgtttgaa atcgcaggcg
    84841 ttcctgaaga agtagctcgt gaagcgttac gtctagcttc tcacaaatta ccaatgaaaa
    84901 ctaagatcgt aaaacgtgag gaaatgggtg gtgaatcgaa tgaaggttaa agaaatcaga
    84961 gaattaacca ctgccgaaat gcttgatcaa gaaaaacaat taaaagaaga attgtttaat
    85021 cttagattcc aattagcaac aggtcaatta gaaaacactg cacgtattaa agaagtacgt
    85081 aaatcgattg cacgcatcaa aactgtgttg cgtgaacaag ctaagtaatt atggaaggag
    85141 gccattaagc gtatgactga aggaagaaat caacgcaaag tttatcaagg tcgcgtggtt
    85201 tcagacaaaa tggataaaac aattaccgtt gctgtcgaaa caaagaaaaa ccaccctatc
    85261 tatggcaaac gcatgaacta ttctaagaaa tacaaagtgc atgatgaaaa caacacagca
    85321 aaagttggcg acatcgtgag aattatggaa actcgtccat tatcagctac aaaacgtttc
    85381 cgtttgttgg aagtagtcga agaagcagtt attatctaat aacacgagat tttcctatat
    85441 agattgaatg caaaatctga aaggaggata cacaacgtga tccaagcaga aagtcgttta
    85501 aaagtcgctg ataactcagg cgcacgtgaa attttgacga tcaaagtcct tggtggatct
    85561 ggtcgtaaaa ctgctaacat cggtgatgtg atcgttgcta cggttaaaca agcaacgcca
    85621 ggtggggttg tcaaaaaagg tgacgtcgtt aaagccgtta tcgttcgaac aaaatcagga
    85681 gctcgtcgta cagacggttc atatattaaa tttgatgaaa atgctgcagt gattattcgt
    85741 gatgataaaa gccctcgtgg aacacgtatc tttgggccag ttgcacgtga actacgtgaa
    85801 aacaacttca tgaagatcgt ttcactagca ccagaagttt tataatcttg agacacttat
    85861 caaaggaggt gcgaaacagt tatgtttgtt aaaaaaggcg ataaagttaa agttatcact
    85921 ggaaaagaca aaaacaaaga aggcgttgta ctagcagcgt tcccgaaaaa agataaagtt
    85981 attgtcgaag gtgtaaatgt cgttaaaaaa caccaaaaac cttctcaagc tgctcctcaa
    86041 ggtggaattg ttgagatgga agcgccaatt catgtttcta acgtgatggt ggttgactct
    86101 actggtgtag ctggccgagt tggctacaaa gaagtagacg gcaaaaaagt ccgtgtttct
    86161 aaaaaaaccg gtgaagtttt agataaataa aagtttaagg aaggagggtc ttactgaatg
    86221 aaccgcctaa aagaaaaata tcttaaagaa gtaactccat cattgatgga aaaatttaac
    86281 tatagctctg ttatgcaaac acctaaagtt gaaaaaatcg tcatcaacat gggtgttggt
    86341 gatgcggtat caaacgctaa aaacttagat aaagcagttg aagaattaac tttgatcact
    86401 ggtcaaaaac caatgattac aaaagctaaa aaatcaatcg ctggattccg tttacgtgaa
    86461 ggaatgccaa ttggtgcaaa agttacattg cgcggagaaa gaatgtacga attcttagac
    86521 aaattggttt ctgtttcact accacgtgta cgtgacttcc atggtgtaag taaaaaagcc
    86581 tttgacggtc gtggaaacta tactttaggt attaaagaac aattgatctt cccagaagta
    86641 gattatgatt tagtagataa agtacgtggt atggacatcg ttattgtaac aacagcaaac
    86701 tctgatgaag aatctcgtga gttgttgaca caattaggca tgccattcca aaaataatta
    86761 aaggaggcga actacgtggc taaaaaatca atgattgcta aaaacaaacg tcctgcaaaa
    86821 ttttcaacac aagcttatac tcgttgcgaa cgttgcggac gtccacattc agtttatcgt
    86881 aagttccatc tttgccgtat ttgcttccgc gaacttgcct ataaaggtca aattcccggc
    86941 gtgaagaaag ctagctggta aaaagtaaat tcgcataaga aggaggtaaa agatcaatgg
    87001 tcatgacaga tccaattgca gattttctaa ctcgtatccg taacgcaaac atggttaaac
    87061 atgagtcttt agaagtacct gcatcaaaaa ttaaacgtga catcgctgaa atcttgaaac
    87121 gtgaaggttt cgttcgtgat gttgaataca tcgaagatga caaacaaggc gtgatccgtg
    87181 ttttcctaaa atttggtaaa aacggcgaac gtgttatcac aaacttaaaa cgtatctcta
    87241 aaccaggttt gcgtgcttat gtcaaagctg acgaagtacc aaaagtattg aacggtttag
    87301 gtatcgcaat catttcgact tctgaaggtg ttgtcactga taaagaagct cgtgaaaaaa
    87361 atatcggcgg cgaagttatc gcttacgtat ggtaatcaag tagaatctac aaggaggtgt
    87421 ctttaagtga gccgtatcgg aaataaagtc gttgttatcc ctgaaggagt aacagttact
    87481 caagacggaa acaacgttac agttaaagga ccaaaaggtg aattgactcg tactttttca
    87541 gcagatatca aaatgaatat cgaaggaaac gaagtaactt tcactcgtcc aaacgacagt
    87601 aaagaaatga aaacaattca tggaacaact cgtgcgaact tcaacaacat ggttgtaggt
    87661 gtcagcgaag gtttccaaaa agcgttagaa ctaatcgggg ttgggtaccg tgcacaatta
    87721 caaggatcaa aacttgtatt gaacgttggt tactcacatc cagtagaaat cacaccacca
    87781 gctggagtaa ctgttgaagt accagctaac acacaagtaa ttgttaaagg tgcgaacaaa
    87841 gaagaagttg gcgaattagc tgcaaacatt cgtggaactc gtcctccaga accttataaa
    87901 ggtaaaggta ttcgttatgt tggcgaatat gtacgccgta aagaaggtaa aactggtaaa
    87961 taatcgttta gaaaaattgc atagaagcca gcagcaggaa aaaggcattt ggctgaagtt
    88021 taccttcaaa aaaatacatt tttacttgtg ccgcagcttc tatttttcga aacgtattta
    88081 ctgcttgaaa tagtagtaca ataaaaataa agaggtgaca attgtgatta caaaaccaga
    88141 taagaacaaa acacgtcaaa agagacatcg tcgtgtacgt aacaatatct ctggtactgc
    88201 tgagcgccca cgcttgaacg tttttcgttc taacaaaaac atctacgctc aaattattga
    88261 tgacgtagcg ggtgtaacgc tagcaagtgc ctctaccttg gataaagaaa tttcaggtgg
    88321 aacaaaaaca gaagcagcac aagctgtcgg taaactagtt gctgaacgtg cagctgaaaa
    88381 aggcattaaa gtagtagtct ttgaccgtgg tggatacctt taccatggcc gtgtgcaagc
    88441 tttagctgaa gctgctcgcg aaaatggact agaattttag aagaaggagg aataccattc
    88501 atggtttata tcgatccaaa acatttggaa ttagaagacc gcgttgttgc aattaaccgt
    88561 gtaacaaaag ttgttaaagg tggacgtcgt ctacgttttg ctgctttagt tgttgtcggt
    88621 gacaaaaacg gacacgtagg atttggtaca ggtaaagctc aagaagttcc tgaagcaatc
    88681 cgtaaagcaa ttgaagatgc gaagaaaaac ttagttgaag tacctatggt tggttcaaca
    88741 atcccacacg aagtaatcgg tgcttttagt ggtggtagaa tcctaatgaa acctgctgta
    88801 gaaggttctg gggttgctgc tggtggacca gttcgtgccg tattagaatt agctggggta
    88861 gcagatatca cttctaaatc tttaggctca aatacaccaa tcaacgttgt tcgcgcaaca
    88921 gttgaaggtt tgaaacaatt gaaacgtgca gaagaagttg cagaacttcg cggcaaatca
    88981 gttgaagaat taatcggata aggaggacaa caataatggc tgaattaaaa gttactttaa
    89041 aacgcagtat tatcggacgt cctcaaaacc aacgcgatac agttaaagcg ttaggtctta
    89101 caaagattaa cagttcagtg gttaagcctt ctaatgaagc aattaaaggc atgatcaata
    89161 ctgtttctca cctagtggac gttgaagaaa tttaatttag aaaaatcata actagaacat
    89221 taaggaggtg ccaaacctat gaaacttcat gaattaaaac ctgctgaagg ttcacgccaa
    89281 gtacgtaacc gtgttggtcg tggtacttca tctggtaacg gaaaaactgc cggacgcgga
    89341 caaaaaggac aaaaagctcg ttcaggcggc ggtgtgcgtt taggatttga agggggacaa
    89401 acaccattgt tccgtcgttt accaaaacga ggattcacaa acgtgaaccg taaagactat
    89461 gcagtcatta atcttgatgt attgaaccgc tttgaagacg gagctgaagt aactcctgtt
    89521 gctttagttg aagctggaat cgtgaaaaac gaaaaagctg gaatcaaagt attagctaat
    89581 ggggaactaa acaagaaatt aactgtgaaa gcagcaaaat tctctaaagc agctcaagaa
    89641 gcaattgaag cggctggtgg atcaatcgag gtgatctaat gtttagacta ttaaaagatg
    89701 cttttaaagt aaaagacatt agatcgaaaa tcctatttac tgtatttgtg ttgtttgtat
    89761 ttcgcctagg tgcacatatt actgtaccag gcgtaaatgc tgcttcattg caaaaccttt
    89821 cttctttgcc atttttcaac atgttgaact tggtaagtgg tagtgcgatg caaaactttt
    89881 ccattttctc aatgggggtt tcaccataca ttactgcttc tatcgtcatt cagcttcttc
    89941 aaatggacat cgtgcctcga tttgttgaat ggtcgaaaca aggggaagtt ggtcgtaaaa
    90001 aactgaatca agcgactcga tatctgacat tagtattggc atttatccaa tctgtcggta
    90061 tcactgctgg attcaatcaa tacgctcaat taggtttcgt gaataatcct aacgtagcaa
    90121 cttacgtaac gatcggtttg attctaacag ccggtacgat gtttgttact tggctaggag
    90181 aacagatcac agaaaaaggt atcggaaatg gcgtatcaat gatcattttt gcaggtatca
    90241 tttctagatt gcctgaaagt atcagagaaa tttatgaaga ttactttata aatattgatc
    90301 cttcagacat atggaaatct gctatttttg tggccatttt actagtagcg gtcttgttga
    90361 tcgtgacatt cgtaacattt ttccaacaag ctgaacgcaa gatcccgatc caatatacga
    90421 aacgcgtagc tggcgcgcct acaagcagct atctgccgtt gaaagtaaat gcagctggag
    90481 ttattccagt tatctttgct agttcgttta ttgctacgcc aaatgctgtt ttacaagcat
    90541 tgagcggtag attcggttct gaaaactggt acaaagtgat gatggagatc tttaactata
    90601 atacagcacc tggtgctacg atttatacga ttctgatcgt tgccttcact ttcttctacg
    90661 cctttgttca agtaaaccct gagaaattag cggaaaactt gcaaaagcaa ggaagttata
    90721 taccaagcgt acgccctggt aaggggactg aagagtatgt ctcaagatta ctgatgcgtt
    90781 taagtacagt tggtgcagtc ttccttggtt tagtggcatt gcttccaatc attgctcaaa
    90841 tggtttggga tcttccacaa tcaatcggat taggcggtac aagcttactg atcgttatcg
    90901 gtgtagcttt agaaactgcg aaacagttag aaggcttaat gctgaaacgt aaatatgttg
    90961 gattcattga gtaagaaagc gtgttgaggt ttcccctcaa cacacgttct cacacattta
    91021 ttaggaggga aaacaatgaa cctcatttta atggggctgc caggcgcagg taaaggaaca
    91081 caagctgaaa aaatcattga tgcttatggt attccgcaca tctcaacagg agatatgttc
    91141 cgcgcagcga tgcaaaacga aacagctctt ggcctggaag caaagtctta tatggataaa
    91201 ggtgcattag ttcctgatga agtaacaaat ggaatcgtga aagagcgact agctgaacca
    91261 gatacagaga aaggcttctt attagatggc tttccacgta cgctagatca agcaaaagct
    91321 ctggacgcaa tgctaaaaga tttgaataaa aaaattgatg ctgtcatcga tatccatgtt
    91381 ggtgaagaaa tcttggtcga acgtctagcc ggccgattta tctgcagcaa ttgtggagca
    91441 acttaccata aaattttcaa ccctacaaaa gttgaagaca catgtgatcg ttgcggtggg
    91501 catgaattct atcaaagaga agatgataaa cctgagacgg ttaaaaatcg tctggctatc
    91561 aacatcaaaa atagtgaacc aatcttggct tattataaag aacaaggttt gctaaacact
    91621 attgatggtg ctcgtgaaat cgatgctgta tttgcagatg tacaaaaaat cattgaaaaa
    91681 tagcacgatt gcccgtagcc gacttgcatt atgcagttac tcttgtcaag ttcaataaat
    91741 tatgatagaa tatgtcagtc cgacttctag agaatcctct agatgatcca ttttgagaat
    91801 tttgaggtgg gaactgcagt ttccgccatt ttatcgtgtg aaaatgcgaa acaagtacaa
    91861 ggaggtactg tgcgtggcaa aagaagatat gattgaagtc gaaggtacag tcgtcgaaac
    91921 tttgccgaat gcaatgttta aagttgaact agaaaacgga caccaagttc ttgctactgt
    91981 ttcaggtaaa attcgtatgc attacatccg tatcttacct ggtgacaaag taacggttga
    92041 attgtctcca tacgatctta accgtggccg tattacttat cgctttaaat aattgtactc
    92101 cgtaaaacta caggaggtat tatcatgaaa gtaagaccat cagtaaaacc aatgtgcgaa
    92161 cattgtaaag taattcgtcg taaaggacgc gttatggtga tttgcccagc aaatccaaaa
    92221 cataaacaac gtcaaggata attggaggtg ctataaatat ggctcgtatt gcaggagtag
    92281 atattcctcg tgataaacgt gtagtagttt cacttactta tatctatggt attggtaaca
    92341 ctactgctaa aaagatctta gctgatgctg gtgtatctga agatgttcgc gttcgtgaat
    92401 taacgaatga acaaacagat gctatccgtg cagaagtaga taaattaaaa gttgaagggg
    92461 atcttcgtcg tgaagtgaac ttgaacatca aacgtttgat ggaaatcggt tcttaccgtg
    92521 gaatccgtca tcgccgtgga ttgcctgttc gtggacaaaa cacgaaaaac aatgcacgta
    92581 ctcgtaaagg cccagctcgc gctatcgctg gcaagaaaaa ataattctta agtgaaggag
    92641 gttaaaactt catggcagca aaaaaagtga atcgtaaacg ccgtgtgaag aagaatatag
    92701 aagcaggtat tgcacatatc cattctacat tcaataacac tatcatcatg atcactgata
    92761 ctcacggaaa tgcattagcg tggtcatcag ctggttcatt aggtttcaaa ggaagcaaaa
    92821 aatcaactcc ttttgccgct caaatggcag cagaaactgc tacaaaagca gcaatggaac
    92881 acggattaaa gactgttgaa gtaacagtta aaggacctgg ttctggacgt gaagcagcaa
    92941 ttcgttcatt acaagctaca ggtttagaag tgactgcaat tcgtgacgtg actccagttc
    93001 ctcataatgg tgcccgccct ccaaaacgcc gtcgtgttta atgagtgccg ctcattttga
    93061 gttttatcag taacgtcatt gaacaagaca cttttcgttt tgaaaggggt aaagataaga
    93121 atgattgagt tcgaaaaacc aagaatcgca aaaattgatg aagaaaaaga ttatggcaag
    93181 ttcatcgttg aacctcttga aagagggtat gggactactt tagggaattc cctacgtcgt
    93241 attttattat cttctttgcc aggtgctgcc atcactaata ttcaaatcga tggtgtactg
    93301 catgaattct ctaccatcaa aggtgttaga gaagacgtca ctcaaatcat cttgaacatt
    93361 aaaggcttag cgctgaaaat gtatggccaa gaagaaaaaa cccttgaaat cgatattacc
    93421 ggacctgcta cagtaactgc tggcgatatt atcgttgata gtgatgtaga aattcttaac
    93481 aaagatatgt acatctgtag tgtctctgaa ggagctacgt tccatgctcg cttaacagtg
    93541 aaacctggac gtggttatgt tcaagcagat gaaaataaaa aagaagatat gccaattggc
    93601 gttcttccag tcgattcaat ctacacacct gttcgtcgtg tgaactatca agttgagaac
    93661 acacgtgttg gacaccgtga agattttgat aaattgacga tggagatttg gacagatggt
    93721 tcaattgaac cgttggaagc aatgagtttg tctgcgaaaa tcatgactga acatttggat
    93781 atttttgtga acttaactga tgaagcaaaa aatgctgaaa tcatggtcga aaaagaagaa
    93841 acacaaaaag aaaaaatgtt ggaaatgacg atcgaagagt tagacttgtc tgttcgctct
    93901 tacaattgtt tgaaacgtgc aggaatcaat actgtacaag aacttacaaa taaatctgag
    93961 cctgaaatga tcaaagtccg taacttagga cgtaaatcat tagaagaagt taaagcaaaa
    94021 ttgcacgact taggtttagg tttacgtaaa gaagattaaa accttttacg aaggagggaa
    94081 accaacgtga gttatcgtaa actaggacgc acatctagcc aacgtaaagc gatgttgcgt
    94141 gatttaacaa ctgatttatt aatcaacgaa cgtattgtga ctactgaagc tcgtgcgaaa
    94201 gaagttcgct caactgcaga aaaaatgatc actttaggta aacgcggaga tttgcatgca
    94261 cgtcgtcaag ctgcggcttt cgtacgtaat gaagttgcca gcgtgcgtga agaaaatgag
    94321 gacatcgtca ttgaatcagc tttacaaaaa ctgttcaacg atattgcacc tcgttacgca
    94381 gaacgtcaag gcggatacac acgcattttg aagacagaac caagacgcgg tgatggtgca
    94441 ccaatggttg tcttagaact tgtctaaaat cacactaata acatcacttt atagatgatt
    94501 cttttagagt gttatgatgt tggaagatat ttttccgagt ctagctcagc gctgctcctc
    94561 aagcgaatat cgcttgggga gtaagcactc atcataaagt gtttttttat accttcaatt
    94621 acctgtcatg acagatatgt ttgaaggcat aaaaaaacat tttaagcgag aaataattga
    94681 aaattttgtc tttttttgat agtgttagta ggaactttag gatgaagaag gagtaatcat
    94741 ggagccaatt attgaattag aaaagataaa ttataaatac cagccagatg atcttcgccc
    94801 cgcattgaaa gatgtctcct ttacaattga taaaggtgag tggatcgcta ttattggaca
    94861 caatggttca ggaaaatcga cacttgccaa aacgatcaat ggattgcttc tgccagaatc
    94921 agggatcgtg aaagtgggaa atcaaatatt ggatgaagaa aatatttgga cgatccgaca
    94981 gatggttgga atggtttttc aaaatccaga caatcagttt gttggttcga cagtagaaga
    95041 tgacgttgct tttggtcttg aaaatcaagg gattcccaga gaagaaatgc tagtgcgtgt
    95101 gaaagatgca cttgagaaag ttagaatggc tgagtttgct tcacgcgagc ctgctcgttt
    95161 atcaggaggg caaaaacaac gtgttgccat tgccggcgtg gttgccttac gacctgatat
    95221 cattatttta gatgaagcaa ccagtatgct agatccagaa ggtcgtgaag aagtgatctc
    95281 tacgatcaag aagatcaaag aagaaagtca attgaccgtt atatctatca ctcatgatat
    95341 tgatgaagca gctaatgcca atcggatact agtgatgaga caaggcgaac ttgtccgtga
    95401 aggtacacca aaagaaattt tttctgctgg tcctgaactg atcgacttag gtctagatct
    95461 accatttcct gaaaaactga agagcgcact gaaagaacgt ggggttgatg tgcccagtga
    95521 atatatgaca gaagaaagga tggtggattg gttatggaca tccgttttga acaagtagac
    95581 ttcacttatc agccgaatac accatttgaa caaagagcct tatttgacat caatatgacg
    95641 atcaaagaaa acagttatac tgcattagta gggcataccg gaagcgggaa atcaacctta
    95701 cttcagcatt tgaatgcgct tgttaagcca acaagcggta cagtccatat tggagaacga
    95761 gatattcagc cggatacgga taataagaat ttaaagccca tccgaaaaaa agttggcatc
    95821 gtctttcagt ttccagaagc acagctattc gaagaaacgg tagcaaaaga cattgctttc
    95881 ggtcctaaaa actttggtgt cagcgaagaa gaagcactag tcctagcaaa agaaacatta
    95941 gaacaagttg ggctggatga aagctatttg gaacgttcgc catttgaact ttcaggaggg
    96001 caaatgcgta gagtagcaat cgctggtgtg cttgccatga ggccggaagt acttgtattg
    96061 gatgaaccaa cggcaggact tgatccccag ggcagaaaag aaatgatgga gatgttttgg
    96121 cgtcttcata aggaacagca gatcactatt gtattagtta cgcatttaat ggatgatgta
    96181 gccaattttg cagattatgt ctatgtgttg gaaaagggac gaatcgttaa tagcggggag
    96241 ccgcaagagg tctttcaaaa cattgaatgg ctaaaagaaa aacaactagg cgtacctact
    96301 gccaccgagt ttgcagaaga attaatggct aaagggatga actttgctac cttgcctttg
    96361 acagctgaag aattagcaga cgctatcgtc cattatgctg gaggaaatgc ccatgatgaa
    96421 taaattgata tttggtcgtt atatgcctgg agattccttg atccatcgat tggatccccg
    96481 agctaaatta ttagccagtt tttattttat cggaatcatt tttttagcaa ataattggga
    96541 aacgtacctt ttgttggcat ttttcacatt actttctgtc ttcttgtcaa aagtagattt
    96601 gaaatttttt atccgaggca ttcgcccatt gatctggcta atcctcttca ctgtagcttt
    96661 gcaggtcttg tttacaagtg gcggagaaac ttattggagt tggggtgttt tccatatcac
    96721 tgaatttggt gtgatgaacg gcctctttat tttctgtcga ttcgtgttga tcatttttat
    96781 gtcgactcta ctcacgctta ctacaccgcc tttggaatta tcggatgcga ttgagtactt
    96841 gcttcgtcca ttgaaagccg tacggttccc agtccacgaa atttcattga tgctgtcgat
    96901 tgctcttcgt tttgtgccta cattgatgga cgaaacagaa aagatcatga acgctcagcg
    96961 cgctcgcggt gttgattttg gtgaaggaaa cctagttcag aaaatgaaag cagtcattcc
    97021 tttgctgatt cctttatttg tcagcagctt caaccgtgcg gaagatcttg cgactgctat
    97081 ggaagcaaga ggctacaaag gtggggaagg aagaacgaaa tatcgtgtat tgcattggca
    97141 taatagagac acgatggtta tcattgcttt tgtattatta actgtgctac tgatcttttt
    97201 aagaggataa acgttcagtg ggccgctatc tattcttttc ttgctgatct gtttgcaaga
    97261 aaggatgcaa gcagtgtatg acaagaaata ttagggtctg tggcagacta aggtcacagg
    97321 cctctttctt ttatctgaaa agaaagaacg aaaaaatttt atagaaaaat cattattcca
    97381 gataaccttt taggaacaaa ggagagaaaa acatggtacg ttacaaagca atcattgcgt
    97441 acgacggaac aaattttaat ggattccaaa aacagccaaa tgggcgtact gttcaagaag
    97501 aagtagaaaa aacattacaa aaaatggcaa atggaaaaga aatcacggtc tttggttcgg
    97561 gaaggacaga tgccggagta catgcgatcg ggcaagtaat ccattttgat tatccagagg
    97621 agcgtccgtt agaacgaatg cgtttcgcgc tagataccca gtcaccagag gatattgctg
    97681 taagacaagt agaaattgtt tcagaagact tccacgcaag atatttggtc aaagaaaaga
    97741 cgtatcaatt tcgagtggat atagggaaac cacgcagtcc ttttcgacgc cattatgcaa
    97801 gttattttcc ttacccactt gatttggaaa aaattcagcg ggcgcttcct gatttattag
    97861 ggactcacga tttcacctct ttttgtgcat caggaagctc gatagaggat aaaatacgta
    97921 cgatctacga ggctaagatg gaggtaaatg aaacaaaaga cgaattgttg tttacattta
    97981 ggggaaatgg cttcttgtat aagatgatac ggatcttagt aggaacgctt ttgaaaattg
    98041 gcaatggccg attgcctgaa gatagtatcc cagatattat tgcgaaaaaa gatcgtaatg
    98101 cagcaggacc tacagcacac cctgaaggac tttatttgta tgaagtgaaa tatgaataag
    98161 aggttatgat agaagtggtc agcttcaaga aataagaagg tatttacgaa aattacttct
    98221 caaaattttg caaaatttcg gcttattttc gaagaagcta cttctggaac accgtttaag
    98281 gaagaggtcg tgaaatcagc gttttgacct gagtattaga gaaacaaacg ggaaaatagc
    98341 tcctcatatt ttgccgtttg ttgagctaat accgaaggtc agctgattga acaccgttta
    98401 agaaagaggt cgtgagcctg tcatttaact ctgagtcaca aagagcaatt tcaaaaaaca
    98461 gcttctcatc tttgatgaat atcgtgactt gtggacgagg cgttgataag tgaatcatct
    98521 ttttgttacc tattttttct gtaaccaaaa aacgatggaa cgttgttttg attgcccttg
    98581 gtgcggacag tctgaacagg ttccatcgtt tctttatttt ctacgtcttc ttctgtgtgt
    98641 gtttcgtggt tgttcttcta ttgctggatc aatcgtgcca taaaaggggc gattgcggtc
    98701 aaggtaccag acataaagaa taaacaatgc aatacatagg acaaaaagga ttgcacctat
    98761 cgtaaatcct tcaggcaagt aactgaaagt gacagtatgt ttacctgctg gaacttttat
    98821 catcaagaat gcgtctttga aattgctgac cttgactggt ttcccatcaa ttttagcttt
    98881 ccatcctttg tcataaggaa tagttgtcac caacatggaa tcttcagcgg tatttacagt
    98941 cccagaagct tttctgccag tggcttccat ttcgacacct ttttctttga tctgattcat
    99001 tgcggcttga taggctacaa catctagacc aacgacttta ggctccatga aacttatttc
    99061 tttcgtccca taaaaacttg cagtaaatgt cactgttgtc tcttgaggat aatagccaag
    99121 atcatagtat tgaccagaaa tgttgatttg cgtttttcgc gatgtaccat tgacagtgat
    99181 cgtcgcacta gaactttcta attgcgcaaa attggttggg aacaaactta agtaagcttg
    99241 tgtatttgca ggcacatgga cttcccaagt gagctctttt ggctggttat ctgctgtttc
    99301 tgagtaagtg acgccagtcg ttgtctttgt gatcgtcgta ttcaactgat tcttcaaagt
    99361 aggtgtatag aatgtaaaat actgttcatt tagaccagac aatgtattga tcaagtttgt
    99421 ctgactggtc aaattatctg tcactggctg ctcaacatca ttaacggaca acggtgcagt
    99481 aaatgctagt ggcaatgcat tgacattctg atacagtgta tagtcgccgc tttgaccagc
    99541 ttggcggaag ccatatttac tgaacgtgct ttcgttttta gaaatgttat atttgattcc
    99601 tgtaaaccca tccatcagca aagtattatt gttataacgg atattcaaat ttgtccctct
    99661 tgaacggaaa cctaatttat ccaggtaaga ggaagaatta cgatttctta tagaagaaaa
    99721 caaactgata ccactgtaac catagttgat cgaatcgttt gcagatactg gatccagatt
    99781 ttccaagcgg taaaacgagt cggaatcttt ttttgtttca tttacaagag ctttcaagtc
    99841 aggataagga tcagtatata aactgcggga agcataattc caatcatcaa gaatgccatg
    99901 gatcatactg ttcgtattga ccagtgcttc agcagacatg atcaataata acaatatact
    99961 cagatgattg aatgaaagtt ttttatgctg ataaaatccc acagcaaaag cataaatccc
   100021 taagaaaacc actgtcaata caaaggaagt catcgtcaca taggtgtagc ttccttcagg
   100081 tttgatgccc catgccaccg caaagagagc agctacgacc aaaatgattc ctaacaacag
   100141 cccacgatcg tcttgttcta tcttttccca tccttttcct gcaagcagga gcactaagaa
   100201 agagaacaga taggcataac ggaataaaaa catattcggt gcatgcatac catgccagaa
   100261 caaattcaat ggaacaagat aaaaacttgc gataagtatg gcgaaaagac tgccgtacaa
   100321 aaatttattt ttcaacggta ctttccgact ggcaaaataa aacagacaga ggactaatgg
   100381 tagcagacct gcgtaaacga acggaatcga tccgtatttt gtcgtgtcat aaacaccaat
   100441 catatttttc atgattagat ccagataaga agtcgcttct gttttcaacg tagtgatctg
   100501 actcagtgcc tcgccatttg accgaagatc cagcactgct ggcaatacaa tgatcattga
   100561 agcgccgcct gccagtaaag aagtgatacc ataaggaaca atcgtttttt tatattctga
   100621 ccagtttgtc aatagacggg cgataaaata taagaaagaa aatacgccaa tcataaaacc
   100681 catataaaaa ctggataaaa acaagagtaa ataactgata aataaaagtt tcggccgttt
   100741 aagatccata acacgatgaa tccccaaaat cactaatgga agataaaaaa atgcatccag
   100801 ccacatgatg atctccgaat gtgcagtgat aaatgagatc aatgcgtaac tgacgctaag
   100861 tgcaacatgc ccccagcgag gaatcttgaa cgtacgtgca agaaaccaaa aagccaatcc
   100921 ggcacagccg accttaagca aagtcagaac atacagtgca tctggcatca tctggtttgg
   100981 aaaaaagaac accagtggcg tgaagatccc gcctagatag taagaaatta aagataaata
   101041 attcaatcct agagaagcat tccatgtata aaaaatactt tgctttccaa gaagaacatt
   101101 tcggaagctg gcatgaaaat tagaaaactg tgaaaaggca tcacttgcca aaatacttct
   101161 gctgcttcca ggatagattc cgatacttag ataaacaagt gctaaaatca aaaacggaat
   101221 caggaaacta gctgctgcgt agcgtccgtt ttctctaaag aatgacttta aatttttttt
   101281 catatttatt ttccaccaaa tttctatgaa gttgtattca gtttatcata tccttgtttt
   101341 tctctgctta aaaagagtct agttttcaca aaaaaataaa atttagaata aataacttca
   101401 taatttgttc gtataacaaa aataaacgca aaaaaagggt aggaatcaaa agtcccaccc
   101461 ttaaaaattt ttattaaata aaaaaccgtt cactatctga acggtccaag tatcattcaa
   101521 ttaaacgcgt tcaacttttc ctgatttcaa agcacgagtt gacacccaaa cttttttagg
   101581 ttttccgtcg accataacgc gaactttttg taagttaggt ttaacagtac gttttgttga
   101641 gttcatagcg tgtgaacggt tgtttccact ttttgtcttg cgacctgtaa agtaacaaac
   101701 ttttgccatg gtaatttcct cctttgcctt gatttggagc aatagaataa ttcagctcat
   101761 accagactaa catatcatat agaatgggaa tttgcaagta cattttcata gatacaattg
   101821 acaattgaga agaataattg taaattaaac aaggttatac ctgaaatttt gcaaggtttt
   101881 tgagtactct gggtaactgc cttttatttc aagttgcgct atgataaaat atgatagaag
   101941 aaaaatggga gttttttacc cgttaaggag gctcattcat ggctgtaaaa attcaaacgt
   102001 cagctggaac cattgaaatt accaatgatg taattgcaac agttgttggt ggcgccgcga
   102061 cagatgtgtt cggtgttgta ggaatggcca gcaaaaatca aattaaagat aatattactg
   102121 agattttgcg taaagaaaat tactcaagag gagtagttgt ccgccaagaa gataatggta
   102181 ttgctgtaga tgtttatacg attatcagct atggtacgaa aatctctgaa gtttcccgca
   102241 acgtccaaga aaaagtgaaa tacaatctag aggcattatt aggcgtaact gccaattcag
   102301 ttaatgtctt tgtacaaggt gttcgtgtac tgccagacta ggcaggagac tggctggtaa
   102361 ctcagcagtc gatctgaagg aggattatct aggtgagtgt aacagaaata agtgcaagcc
   102421 agttccagga aatggtccag gcaggtgcga atcgtctgca agtaaatgca gaatacgtca
   102481 attcattaaa tgtattccct gtgccagatg gcgatacggg aacaaatatg aatctatcta
   102541 tgactagtgg agctaaggca gtagtagatt caacctcaga aaaagtagga gaattagcag
   102601 caatgctttc aaaaggattg ctgatgggtg cgcgcggaaa ttccggcgtt attttgtctc
   102661 aattgtttcg tggattttct aagcaaattc cagatgttgt ctcattaaat gcttctgatc
   102721 ttgctgcagc cttcactcat ggagttgaaa ctgcgtataa agcagtaatg aaaccagtag
   102781 aaggaacgat tttgacagta gctagagaag cagcgaaagc tggagaaaag aaagcaaaaa
   102841 gtactgatga tgtcgttgaa gtaatgacag ctgttgtaaa aggagcaaag cgtgctttag
   102901 ctaaaactcc agacttgctt ccagtcctaa aagaagtcgg tgtcgtagac agtggagggc
   102961 aaggactgct gtttgtttat gagggctttt taagtgcatt gaatggagaa ttccaagcgg
   103021 atgaaacata tgaaccttct ccagcagaaa tggatgatat ggtcaatgca gaacatcatc
   103081 gcagtgtcca aggtcaaatg gcgacagaag atatccattt tggttactgt acagaaatca
   103141 tggtaaaaat cggagaagga cctacagtcg atagtacatt cgattacgaa gaattccgta
   103201 actatttaga tggtatagga gattctttac ttgtggtcaa tgatgacgag atcatcaaag
   103261 tccacgtgca tacagaaaac ccaggagaag tcttgaatta tggacaaaaa ttcggttcat
   103321 tagtcaaaat caaagtcgac aacatgcgct tgcagcatga gacgattttg gaacacgatg
   103381 aagaagtgaa cgagtttgcg aaaaaagaaa taccacgtat cccgtatggt atcgtagcga
   103441 tcgctgctgg taaaggcgtc caagagctgt ttgaaagttt aggcgctaat tatgttatca
   103501 gtggtggaca aacaatgaat ccaagtacgg aagacattgt taaagcgatt caagaagtaa
   103561 acgcagataa agtgattgtc ttgccaaaca ataagaacat cttcatggca gctgatcaag
   103621 cagcagaagt agcagatatc ccagtagcag ttgttccttc aagaacgatt tcccaaggat
   103681 tgacagctat gctttcattc aatgatcaag cttctttaga agaaaacaaa gaaacgatga
   103741 cagaaatgtt gtctagcgtt gtgagcggtc aagtcactca cgcgatcaga gatacctcaa
   103801 ttgacggtgt agagatcaaa gaaggagact ttttgggcat gatcgatgga aaaatcctga
   103861 tctctaaccc agatatcaca gcaacagcgt taagtacact agaacaaatg atcaatgagg
   103921 acacagagat cgtaacgatc ttggcaggtg atgaaggaac agttgaacaa gcaaatgaat
   103981 ttgctgagaa actaacagaa aaacatcctg atttggaaat cgagatccac caaggagatc
   104041 agcctgtcta tccttatcta ttttctgctg aataatttct gtgtcagaat atctttctaa
   104101 cacagtattc attcataaaa ggccttcggt tagtgatcag gtatgatcgc tgaccaagtc
   104161 ctttttttgt taaaggaggg aggaaaaatt tatgcgttct ttagatgatc caatcactat
   104221 gctggcagga gtcggagaaa aaagagcagc tagtcttgcc agcttgggca tccaaacgat
   104281 tgaagactta ctgacttatt atccttttcg ctacgaagat attcaggaaa gaaatctaaa
   104341 cgagatccag gatcaagaaa aggttacttt gaaaggaatc gtagtctcgc ctcctgtgat
   104401 gaatcgattt ggttataaaa aaagccgatt gcaatttcgt atgatgcaag aacatgatgt
   104461 gttcaacgtt tcttttttta atcaacctta tctaaaagaa aaagtcattt tatcggagga
   104521 aatcgccgta tacggtaaat gggatgcaaa gcgtaaagcc ttgaacggga tgaagatttt
   104581 aggttctcaa acaatggatg acttttcgcc gatatatcac gtcaacaaat caattcgtca
   104641 aacgacatta gttgatctaa tcaaaaaagg gtttcaagaa tacggcacat tgattgaaga
   104701 aaatctgcca aacaaccttg tggagaaata tcgactgctt gaccgagctt ctgctgtaag
   104761 aagtatgcat tttccaaaag accacgaaga aaaccatcaa gccaaacgac gtgtcgtttt
   104821 tgaagagttc tttttatttc aaatgcgtct tcaaggattg aaaaaagcag aaaaagcaga
   104881 aacgaatggt ttggagatcc tttatgatgt ccaaaaattg aaacaattta ctcaaaagct
   104941 accttttgaa ctgacaaatg cacaaaaacg tgtaacgaat gaaatctgtc gggatcttcg
   105001 cagtccgcag catatgcagc gacttttgca gggagatgta ggtagcggaa aaacagtggt
   105061 tgccgcgatt gcgttatacg ctaccgttac agcaggtttc caaggtgcgt tgatggtgcc
   105121 tacagaaatc ttggcacaac aacatatgga aagcttgcaa cagctgtttg atcccaatga
   105181 agtaaccatc gcattgatta cggggtcaac gaaagcaaaa gaacgacgtg agctccttga
   105241 aaaactagag caaggggaaa ttaacgtcgt gatcggcaca catgcattga tccaagatgg
   105301 agtcgaattt cagaatttag gtctagttat tacggatgag cagcaccgtt ttggcgtgaa
   105361 ccagcggaaa gtacttcgtg aaaaaggctg gcgaccagat gtcttattta tgacagcgac
   105421 tccgattcct agaacattag ctattacagc atatggtgag atggatgtat ctatcattga
   105481 tgaacttcca gctggacgta tcccgatcga aacacgctgg gtaagaacac ctcaattgga
   105541 cagtgtactg gattggacga aaaaagaact ggcaagagga catcaaatgt atgtcatctg
   105601 tccgctgatt gaagaatcag aagcattaga tgtaaaaaat gcagtggaaa tctatgaaaa
   105661 acttcgtgaa ctatttgcac caaaatatga agttggcttg ttacatggta aaatgaagaa
   105721 tcaggaaaaa gaagtaatca tggaagcctt caaagacaac cagatgcaga tattagtttc
   105781 gactactgta attgaagtag gggtaaacgt gccgaacgcg accgtcatgc tgatcatgga
   105841 tgcggatcga tttggattgg cacagctgca ccaacttcgt ggacgtgttg gacgcggaag
   105901 tgatgcttcc tactgtatct tagttgctaa tcctaaaaat gaactaggtg tggaacggat
   105961 gaaaatcatg acagagacca ccaatggttt tgtactaagc gaaaaagatc tggaattacg
   106021 tggtcctggt gaagtatttg gcttccgtca atctggtctg cctcaatttg cgattgctga
   106081 cttggtgacg gatggaaatg ttttggaggt agccagagaa gaggctgcag ccctttggca
   106141 aataaaggat tggcagatgc ttcctgagta tcagccttta gcgcaaagcc tccaaataga
   106201 aaatcaggat aatcgttatt tcgattagca gactgtgcta tactttatac tgatgacgga
   106261 aaaaggagtg gaaacaatga aaattgccgt agatgcaatg ggcggagatc acgcacctca
   106321 agcaattgta gaaggcgtgg ctttagcgca gcaagatttt ccagaagtag aattcttact
   106381 ttacggaaaa gaagcagaaa tcaagaaata cttaacaaat gaaaaaaaca tcacgattat
   106441 ccatacagac gaaaaaatca atagtgatga tgagccagta aaagcaattc gacgaaaaaa
   106501 gactgcatca atggtccttg ctgcacaagc agtgaaaaac ggtgaagcag atgctgtatt
   106561 ttcagcaggg aatacaggcg cgctactagc agcagggttg ttcatcgtag gacgtatcaa
   106621 aaatatcgaa cgccctggct tgatgtctac cttgccagta gtagggaaag aaggtgccgg
   106681 tttcgatatg ctggatcttg gcgctaacgc agaaaacaaa ccagaacatt tgttgcaata
   106741 cggtatttta ggttcttttt atgcacgtaa agtccgaggt atcggacgtc ctcgtgtcgc
   106801 actcttaaac aacggaacag aagcaacaaa aggtagtgaa gtgacgaaaa aagcctatga
   106861 actccttcag aatgaaacaa gcttgaattt tattggaaac gtagaagctc gtgagctact
   106921 caatggtgtg gctgatgtag tggtcacaga tggatttaca ggcaacgctg ttttgaaatc
   106981 tattgaagga acagcaatga atatgatgaa tctattaaaa tctgctattc taaatgaagg
   107041 gatcaaagga aaaatgggcg ctatgctttt aaaagacggc ttacgttctt tgaaagcaga
   107101 aatggattat tccaaacatg gcggtgccgt tttgtttggc ttgaaagcac cagtcattaa
   107161 aactcatgga gcaacaggcc ccgatgctgt tcgctatacg atccgccaaa tccacacaat
   107221 gcttgagaca gatgtagttg gacaattagt cgaacaattt gaaaaaagcg aagaatagta
   107281 tttggatatc tgaagcgatc cgttccattg aaaaaaaggt ggacattcct gaaaaatgcc
   107341 agtaaaatta gccatggtgt aggtaattga caagtgtatg attcttggag gtgaaaaagt
   107401 tgactcgcga agaagtattt aacaaagtag cgaaaattat ttcaaatcat tttgagatag
   107461 ataccgataa agtgacggat gaattgaaca tcaaagatga tttaaaagct gattcaatta
   107521 gtattatgga attcgtttta gagcttgaag atgaattcgg tacggaaatt tcagatgaag
   107581 acgctgaaca aattgaaaca gtcggtggcg cagtagatta tatcagcagc catcttaaat
   107641 aatttcaaat aaaagcaaag aaggataaaa agctgcatgc agctttttat ccttcttttt
   107701 ttaatctttt tgacacgtaa aagtcatttt tctgataatt ctggcgataa acagaaatta
   107761 tgttgcaaag taaagcggtt ttctatataa ttattaataa ttcattacac aaagataaga
   107821 ataacctttg ttataaaacg atttattctg tgtaatgtcc ttttttatta agaaaaactt
   107881 ccatttagga agcagagagg ggtattcgat tgtccgataa ccaattatta gaagttcaga
   107941 acctacatac aggattccga ctcaaagatg actattacga tgcagtagat gacgtttcat
   108001 ttactttaga caaaaacgag attttagcaa tcgttgggga atctgggtgc gggaaaagta
   108061 ccttggctac tacgattatg ggattacttg atcctaataa cacaaaaatt acaggtgaga
   108121 ttttatataa cgatctgaat ttaatcgacc tgaacgaaac gctctataat aaaatccgcg
   108181 gaaatgatat cggaatgatt ttccaagatc cgttgtctgc attaaatcct ctcatgagaa
   108241 tcgaagacca gatcaaagaa ggactttcct atcacacaaa gatgaatgca gaacagcgtc
   108301 aagctcgtgc actagaattg ctagaacaag tagggattcc taatcctgcg agagttggac
   108361 gccaatatcc ccatgaacta tcaggcggta tgagacaacg tgtcatcatc gcaatcgcaa
   108421 tcgcctgcaa acctccaatc gtcattgcag atgaaccgac gacagcccta gatgtaacca
   108481 tccaagcaca gattcttgat ttactgaaag accttcagga agaaacaaaa acagggatca
   108541 tcttgatcac tcatgactta ggagtcgtag cagaaatggc tgatcgagtg gcagtgatgt
   108601 atggcggaca gttcgttgaa gtagcaggtg taaaagaact gtttgatcat cctaagcatc
   108661 cttatacacg ctctttactg aactctatac cacaagaagg aaatcatgat gcagaactcc
   108721 atgtgatcga aggtgtcgtt ccttctctga aaaacatgcc tcgtaaaggt tgtcggtttg
   108781 caccacgtat tccatggata ccagcaagcg atcatgaaga agagcctatc cttcatgaag
   108841 tcgcaccgaa ccacttggta cgttgctcct gctataagca tttccacttt agagacgaaa
   108901 aaggagaggt gtagatgaca gaattaatca caatcaaaga cctgaaagtt cattacccta
   108961 ttcgcagtgg attcttcaat cgagtaacgg atcatgtata tgctgtagat ggtgtggatt
   109021 ttattatcga aaaaggaaag acatatggtt tagtgggaga gtctggctct ggaaaatcta
   109081 ctacgggaaa agccgttgtg ggattagaaa aggtaacagc cggacaaatc atgtatgaag
   109141 gaaaagatgt aacgaagcga agcaaccgca aaaaaatggg ttataacaag gatgtgcaga
   109201 tgatcttcca agattcaatg tccagtttga atcctaaaaa gcgagtgttg gatatcattg
   109261 ctgaacccat ccgaaatttt gaacggttaa gcgatcaaga agaaaagaaa aaggtaaaag
   109321 gactgctaga tatcgttggg atgccggaag atgcgctata taaatatcct catgaatttt
   109381 caggcggaca acgtcaacgt ctaggagtag cccgtgctgt tgctaccaat ccaaaactga
   109441 tcgtagccga tgaacctgta tctgctctgg atctatctgt tcaagcgcaa gtattgaatt
   109501 tcatgaaacg aatccagcaa gaatacggcc tcagctattt attcatttca catgatttag
   109561 gtgtagtaaa acacatgtgc gacaatatcg caattatgta caaaggtcga tttgtagaaa
   109621 tcggtacaag agaagacatt tataatgacc cgcgacatat ctatacgaaa cgtctgcttt
   109681 cagctattcc gcaaatcgat gtagaaaata gggaaagcca caaagaaaat cgacgaagag
   109741 tagaacaaga gtatatccaa aaccaaaaag aatactatga tgcgactggt cgagtctacg
   109801 atttgcgtac gatcacaact acacacaaag tggcgttgaa agatggaggt gcaagctaat
   109861 gtggaaaacg attctgcgac gtgttttatt gatgatcccg caagttatta ttttaagtgt
   109921 tctgatcttc ttattagccc aagcgatgcc gggggatcca tttaccggtt tgatcaatcc
   109981 aaatcaggat ccggcagtga tcgaacagat gcgacaagca gctggcttga atgacccatg
   110041 gtatgtccag tacttccgtt ggatcggaaa tgctttgcaa ggcgattttg gtcaaagttt
   110101 cttatacaaa cgctctgttg ctgacttgat cggcgaacgg atcatcaaca ctttatatct
   110161 atcgatcttg acagttgtcc ttacttattt gattgctgtg cctctgggga tgcttgctgg
   110221 ccggtatcaa aattcaatct tggataagat tgtcgtaatc tacaacttct ttagttttgc
   110281 tgttccttta tttatcttcg gcttgatcat gctatttgtt tttggttatc gtttaggctg
   110341 gttcccaaca agtggatcac aaacactagg tttgcaaggt ggatttatca ctcaatggat
   110401 agaccgtttg aaatatattc tgttaccatc aattacccaa gcatttttgg cgactgccgt
   110461 cacaatccaa tatctgcgaa gtgaagtaat cgattcgaaa tcgctcgatt ttgtcagaac
   110521 agcccgttcc aaaggggtac cgacgaataa agtattcagt cgacatattt tccgaaatgc
   110581 agcgttgccg atggcttctc aaatggggta tgaaatcact tcgttgattg ctggttctgt
   110641 agttatcgaa aagatttttg gttatccggg agtcggaaaa ctatttattg attccatcgg
   110701 ccaacgggac tataccgtga tcacatcctt agtattgatt ttaggaatcg cgacgctagt
   110761 ggggacatta ctttcagata tcatcatgag tatcgttgac ccgcgtattc ggattcaata
   110821 aaaccaatca gaaaggagaa tggatcgatg agtgaaaaaa acaatacagc agttcaagaa
   110881 agtattcctc ctatggggat acgaatgatt gctcgagaat ttaaaaaaga taaaattgct
   110941 attttttcat taggtttgct agtggtttta ttattagtga tctttatcgg tgcgatgctg
   111001 accgatcaag acaaagtgat gtatgttagt atcttcgaca aatacgctgc accaggaaca
   111061 gtttcgacac aaggacaaaa gttcattctg ggagcagatg aaggcggacg tgatgtatta
   111121 ggacaattga tcataggtgc tagaaactct gttacgatcg gatttgcgat cacgatcctt
   111181 acttcgatca tcggggttgg attagggatc atctctggct actacggagg gatcattgac
   111241 aacatcttga tgcggatcgt tgattttatc atgattctac cgatcatgct gatcattatc
   111301 gtcttcgtat cgattatttc cagatacaat gtctggtcct ttatattcat catgagtgca
   111361 ttttattggg tagctaaagc ccgattattc cgaagcaaga cattatctga agcaagaaga
   111421 gattatgtca gtgcctcaaa aacaatggga accaatgatt ttaaaatcat gttccgcgaa
   111481 atcatgccga atctaagttc attaatcatt actaatttga cgatgaactt tgcggcaaac
   111541 atcggtatcg agacaacttt gactttctta ggtttcggct tgccggcaaa tgtacctagt
   111601 ttaggtacat tgatcggtta tgcaagtaac ggagaagtct tagtgaatcg gcaatggatc
   111661 tggttacccg catcaatcct tattttagtt ttgatgttga gtatcaatta tgttggacaa
   111721 gcattcaagc gttctgcaga tgcgcgccaa cggttaggct agtatagaag cgaaggggga
   111781 aataggaatg aagaaaacga tttttggatt tgcagcgctg atgtctgctg tcgttcttgc
   111841 agcatgcggg ggcggaggta cagatacctc tggaaacaac tctggaggca acggcaataa
   111901 caaagaggaa ttaacttttc caattgcaac aaaaaataat gatgatgcaa tcgaagacgg
   111961 cgaattagaa gctgcggtcg caacggacag tcagtttaaa ggattgttcc agtgggagtt
   112021 ttaccaagat tcatatgatg cagcttttat ggctccttct catgaaccgc tatttgcaaa
   112081 cgatgacgat ttcagaatca cagacgacgg agctgcaacg cttgatctag atgaagatac
   112141 gaaaaaagcg acaatcaaag taaaagacaa cgtcaaatgg tcagatggtg aagatgtaac
   112201 ggcggacgat gtgattttcc cttacgaaat cattgggaat aaagattaca ctggtatccg
   112261 ttatgacgac actttccgta acatcgtagg tatggaagaa tatcatgatg gaagtgccga
   112321 cacaatctcc ggaatcaaaa aaatagacga caaaacagtt gaaatcgaat ataaagaagt
   112381 aaacccaagc atgcttcaat cgggtggtgg tatctggtca tatgcagcac caaaacatac
   112441 gttagaaggt gtagcaatca aagatatgga atcaagcgat caagtacgta aaaatccagt
   112501 gacttttggc ccttattaca tgagtaatat cgttgcagga gaatcagtag aatacttgcc
   112561 aaacgagcac tactggaatg gtacgcctaa attgaaaaag atcacgatga aatctgtacc
   112621 gatggcaagt gcaactgaag cattgaactc taaattatat gatatgattt tccaaatgcc
   112681 aacagatact tacgatacct ataaagaaat tgctggctat acaaacttag gccgtgaaca
   112741 aacttcttat acttatctag gattcaagtt aggtaaatgg aatgcagata caaagagtgt
   112801 ggaatacaat ccagattcaa aaatggctga taaatcatta cgtcaagcaa tgggctatgc
   112861 attagataat aatgcagtag gtgaacgttt ctatgcaggt ttgagaagca atgcgacttc
   112921 attgattcct ccagtattcc agtcatacca tgatgatgag atgaaaggat tcacacaaga
   112981 tctagacaaa gcgaaaaaac tattagatga tgctggctac aaagatgtgg atgacgatgg
   113041 tttaagagaa gacaaagatg gaaataaact aacaatcaac tttgcttcga tgtccggtgg
   113101 ggaaacagcg caaccattgg cagattacta tatccaacaa tggaaagaaa ttggtttgga
   113161 tgtccaatac acaacaggtc gtctgatcga attcaattct ttctatgata aattagaaaa
   113221 cgatgatcct gaaattgatg tttaccaagc tgcttggaat acaggaactg atccaaatcc
   113281 aagtggacta tggggaccaa atgctgcgtt caactacact cgttatgaat cagaagaaaa
   113341 tacaaaattg atcaacgata tcagttcaag caaagcattt gatgaagagt accaaaaaga
   113401 agcatatcaa aaatggcaac aatatgcatt tgaagaagca tttgcgattc caacgttgtt
   113461 ccgtaatgaa gtattgccag tcaacgaccg tgtcaaagac tgggattggt cttatgatgt
   113521 accaaaccca tgggcaaatg tttctgttac agcagattca cgttcataat cactaaaaat
   113581 gaccgaaaaa gggcgatgtc ctttttcggt tttttgatat ttttctgttt atttctctaa
   113641 tattcaggtc aaggcgctga tttcatgact tctccaataa acggtgttca aagtccagtt
   113701 tctcggtatt aagtcaaatt tatgcaaaaa tatggaaagc cattttcaca aaattttttc
   113761 ttcatactcg aaactaaacg aatttttcac aacttctcgt caatcactag gattattaaa
   113821 gctttactta ccttttgacc gaaccatttc aaatttgaca aaaaaccagt ataataagga
   113881 agtatcgaaa aaatatccat aaaaggaatg tggaaaatgg acaaccagct gacaaaagaa
   113941 ctaaaagaaa actataacat cgtttttcac gatctaaatt tgcttgaaca agcttttacc
   114001 cattcatcct atgtgaatga gcatcgcaat ctgcaattat cggataatga gcgacttgaa
   114061 tttttaggag atgccgtttt agaactgatg gtttctcagt atttattccg tttatatcct
   114121 catttgccag aaggaaaatt gacaaaaaca cgagctgcga tcgttcgcga agacagcttg
   114181 tcgaaatttg ccaaagaatg tcactttgat caatatgttt tgttgggcaa gggagaagag
   114241 aattcaggag gacgtacgcg accagcattg ctttgcgacc tattcgaagc ttttttaggc
   114301 gcgttatatc ttgaccaggg attcgacacg acccatgcat tcatcgaaaa agtgattttt
   114361 cctaaagtca aagcaggtgc tttttcacat gagatggatc ataaaacaaa actacaagaa
   114421 gtcttgcaaa aatcaggtga tgtatcaatc gaataccgat tgatcaatga agaaggtcca
   114481 gctcatgaac gagtattttg gatcgaagtt tatgtagatg atcaattgat tggcactgga
   114541 caagggaaat cgaaaaaatt agcagaacaa gctgcggcgg aaaatgcatt ggcagcactg
   114601 taaaagtaag tgagggaaag ctttgtattt gaaacgaatt gaaatagccg gttttaaatc
   114661 atttgcagac aagacagtca ttgattttga aaatagcgtc acagcagtgg ttggaccaaa
   114721 tggcagcgga aaaagcaata ttacagaggc tatccgctgg gtattaggag aacaatctgc
   114781 taaaagtctg cgaggcggaa aaatgccgga tatcatcttt gccggttccg atacccgtaa
   114841 gcagctgaat attgcagaag tcacagtgat tttagacaat agtgatcatt atctgccatt
   114901 ggagtacaat gagatcagtg taacccgccg ttatcgacgg actggggaaa gcgagttttt
   114961 catcaataaa caaagctgca gattaaaaga tatccaagaa ttgttcttag attctggatt
   115021 aggaaaagaa tcgttctcaa tcatttctca aggaaaagta gaagcgattt tcagtagtaa
   115081 accggaagac cgtcgtggga tctttgaaga agcagctggt gtcttgaaat acaagcagcg
   115141 gaaaaagaaa gcagaacaga agctttttga aacagaagac aacctcagcc gcgtccaaga
   115201 catcatccac gaattggaag aacagttaac tcctttggcc gcccagagtg aggcagcaaa
   115261 ggagttttta cggctaaaag aaacactgac tcaaaccgat gtctctttga tggttgctga
   115321 aatcaagaca gcaaaaaaag attgggacaa caaacaagca cagcttgcga aattcaatct
   115381 agaattaggc aaactatctg agtcgatcca agaacaagaa tccattttgg cgaagcagcg
   115441 taaagaaaat gcgcaagccg atcgtctgat cgaaaaaaat cagcaagtat tattagatct
   115501 ttcggaaaaa ctgaagcaga cagaaggaca aaaagatgtt ttgcaggagc ggacaaagca
   115561 tacacaaaaa agctcgcaag aatatcaaac ttcattagca gaagcacaaa aaaaggttaa
   115621 acattttgaa aagcttcaag aaagcttgat gaaagcagca gcagaaaaag aaacagagat
   115681 ccaaaaagct gaagcaaatc tcataaaaac acagcaagaa ttagaaaaat accaaaaatc
   115741 aacgaaagaa ttattggcag aattaagaga tcaatatgtt gatttgatgc aagaacaagc
   115801 agctgtagga aacgaactga agtatctaga acgtcaatat atacaagaaa cagcaaaaag
   115861 caagcagacg ttagccaagc aatcggaagt agaagcatca gtagaccgat tgatcttaca
   115921 aaaagaagaa ttgacacaaa agcaggcgca gctaaaatca tctttaactg aaacaaaaga
   115981 gaagttagaa atgatccaac aaaacgggaa aaagttccaa gaagcattag caaaagaaca
   116041 gcctaaaatg tatcaattaa tgaatcaagt ccagcagcta cgtgctcgta agaaaagcct
   116101 tcaagaaatc caagaaaact attttggttt ttaccaaggg gttcgattgg tcttgcagca
   116161 taaacagcag ctttcgggta ttgtcggagc agtggctgaa ctgatcgatg ttcctgcaga
   116221 tttcacgtta gctatcgaag cagctttagg tggggcagcc cagcatgtca tcgttgaaaa
   116281 tgaaaaagat gctcggcaag ccatcacgta tttgaaacag caaagaggag gaagagcaac
   116341 attccttcca ctgacgacaa tcaaaccaag acaattaccg gctcatatat tgacacaagc
   116401 agcagctgta gaagggttta tagggattgc cagtgaacaa gtcagttatc ccgatcaaat
   116461 ccaaactgtg gttcaaaacc tgctaggaac gatcctttta gccaaagact taactagtgc
   116521 caatgcaatc gcgcaaacga tccgctatca atatcgagtg gtttccttag aaggtgatgt
   116581 gatgaatgcc ggaggatcca tgacaggtgg agcgaataaa cgaggaaacc aaggcagttt
   116641 gtttgtacaa aatcaagaat tgaaacagtt gactagtgaa tttgaagaag cagataaaca
   116701 actccaagca caagaaaaaa aagttcagga gctgcaacaa gaaacagctc gtttagccga
   116761 agaacaagaa gttttgcgta cacgaggcga acaattacgt tttgaagaac aagaagcgac
   116821 aaatcagcta caaaacatca taaatgaact tgaacgtttt gaaaaagaaa agcaaatctc
   116881 aacttttgag acacgtgaac tgcagcagtt tatcgaagat tacgaaaagc aaacgaacga
   116941 attgaaagac aaacaaacag atcttgaatc gcaacgacaa aaaatcgacg aagaaatcaa
   117001 atcgttaagt caagaaagcg accagatgga agccagaaga gcacaagtac aaagtcaaaa
   117061 agcacaagaa caagcagatc ttgctgtctt gaaagagcag tttaatcatc tgcagatcca
   117121 gctccgcggt gcccgagtcc aaaaagcaga ggcaaccgaa cgtcaagaag cgatagaaaa
   117181 gcagttggcg acattgacag cagacttttc tgatcatgaa gtcacagaag aaagtttgga
   117241 aaaacaaatt aacgaattat ctgctcagcg agaaacatta aaagccgagt tggcaaaagc
   117301 caaagagcag cgagatagaa ggcaaaagga gatcgataag cttgaagcag tcttggcaga
   117361 gcgtaatcgt gagcaaaaag cacgactgtc ggaacaatcc aaattagaag tccaaaaaga
   117421 ccgagctgaa atgattttag ataatcattt gacctatctg cagtcagaat atcagctgac
   117481 atttgagaaa gcttcccaag actatcaaga aacaaccgat atcgaagaca gtagaacaaa
   117541 ggtttcatcc ttaaaagaac agattgaaaa actgggacca gtaaacttaa attcaatcga
   117601 acaatacgaa caagtcagtg aaagacatac attcttagca acacagcgag atgatctgtt
   117661 agcagcgaaa aaccaattgt ttgaaacgat ggatgagatg gatgatgaag tgcggacaag
   117721 attcaaagaa gtatttgaag ctatccgtca agaattcaaa attgtctttc caaatatgtt
   117781 tggcggcggt aaagctgaat tagtactgac ggaaccaact gatttgctga aaacaggtat
   117841 tgagattgaa gtacaacctc ctggcaaaaa actgcaaagt ctaagtttgc tttctggagg
   117901 agaacgagca cttacagcca tcgcgttgct cttttccatt atccgtgtct gtcctgttcc
   117961 gttttgtatc ttagatgagg tagaagcagc gttagatgaa gccaacgtga aacgttttgg
   118021 tcgttacttg agcgatttcc aagatgacac acaatttatc gtagtcacac accgcaaagg
   118081 aacgatggtc gcagcagatg tactgtacgg agtgacgatg caggaatccg gtgtatcaaa
   118141 aatcgtttct gtacggatgg aagatatcaa tgaagaggcg aaaatcaacg aaacgaaagt
   118201 gtaggaacga aaatgatcaa attagtagca atcgatttgg acggtacatt attaaatgaa
   118261 caaaagctga tttcagatga gaataaacaa gcattagcac aagcaaaaca gcaaggggtc
   118321 aagatcgttc tatgtacagg acgcccctta gctgcgatgg cgcattatct gcaagaatta
   118381 ggactggtag atgaaggtga ctacagcatc actttcaacg gcggtttggt acaaaaaaat
   118441 gatactggtg aaatcatgga aaagaaagtg atggaagtat cagatatcca acgcttatac
   118501 aaactggctc agcaattgga tctgccacta gatgttttgt ctgacaatgt ggttttacag
   118561 ctgcctagtg ccccaaggaa gcaatccttg tacaatgtgt tgaataagct gctgcaattc
   118621 caaccagcgg atataagcga tattacagat gaatttgtgc ttaataaggc agtaatcgct
   118681 tatgaccaag aagaattaga tcctaaaatc aaagaaatac cagcagaatt ccatgaaatg
   118741 tatgagatca tcaaaacaag aagtatgctt ttggaattta tgccaaaagg tgttacaaaa
   118801 gcttatggta tttctttatt ggcaaaagat cttggtttga aacaagaaga aatcatggcg
   118861 atcggtgatg aggaaaatga tttaccgatg atccaatatg ctggactagg cgtagcgatg
   118921 gagaatgctg tgcctttcgt caaagaagca gcagatttcg tgacagccag caatgaagaa
   118981 aatggcgtag ccaaagtgat ccaaaaattt gtattagagt agggggagtc aaatgggatt
   119041 ttttgataaa atcaagcgtg cttttactgg cgaagagaaa acagaagaag ttgaagtaca
   119101 ggagaagtac gacaagggtt tagaaaagac gcgtaaaaca tttggtgaac ggatgaatga
   119161 gttgtttgcc aattttcgaa cagtcgatga agactttttt gaagaattag aagaaacact
   119221 tatcggtgca gatgtaggtt ttgaaacagc gatacgaatc accgaagcat tacgtcaaga
   119281 agtgaaactc agaaatgcga aaaaatcggc agaagtccaa aatgcgatca tcgagaaact
   119341 tgtggatctc tatgaagcgg aaggaatcaa tgaagtaaat gaactaaata tccaagaaga
   119401 cggactaaca gttattttgt ttgtcggtgt taatggtgta gggaaaacaa caagtattgg
   119461 taaacttgcc catcagttca aaaacgaagg taaaaaagta ttgatggcag cagcagatac
   119521 cttccgcgct ggagcaatcg accagttagt tgtctggggc gaacgcgctg gtgttgaagt
   119581 tgttcgtggc aatgcaggag gcgatccagc agctgtcgta ttcgatgctt tggaaagagc
   119641 aaagacagag caggcagatg ttttactagt tgatactgca ggccgactgc aaaataaagt
   119701 gaacttgatg aacgaattag aaaaaataaa acgagtaatc cagcgggaat taccagaagc
   119761 accccaagaa acacttcttg tattagatgc aaccactggt caaaacgcga tggttcaagc
   119821 aaaacaattc aaagaaacaa cagatgtgac cggtcttgtt ttgacaaaat tagatggtac
   119881 agcaaagggc ggtatcgtac ttgctatccg taatgaattg catttaccag tgaaattggt
   119941 cggtcttggt gaaggaatcg atgacttgga gccgtttgat ccaaatgatt ttgtcgttgg
   120001 attgttcaaa ggattactaa aagaagaata acccatgtct aaaaagcagg agctgtgaca
   120061 aaagttgcag ctccttgttt gaatgattgt ttttaacata gctgtgtttt tgatgccttc
   120121 atttttatac ttatttgtaa gcggttctca aacaatttcg ttctttttct tagacttttt
   120181 gattataatt agattcatat gaattggagg tcacttaatg atgaatttta agcaattatt
   120241 attgcatgtc aatgcgcgcc cttttatcga ccaagctcga tttggtatag aacgggaagg
   120301 acagcgggtt gatcttgcag gaaatctagc aaaaaccgat catccagcaa tctttggcga
   120361 tcgatcctat cacccctata tccaaacaga ttttagcgaa acacaaacag agatgatcac
   120421 ccctgttacc gattctattc ccgaattatt tcagtatctc gctgctgttt atgatgtgac
   120481 tgctcgttct ataccgaaag aagagatgat ctggccatta agtatgccac ctgccttacc
   120541 ggaaaaagac gaagagatca ttattgcaaa attaaaaaat ttcgaagatg tcttgtatcg
   120601 acgttattta gcaaaagaat acgggaagcg aaaacagatg gtaagcggta ttcatttcaa
   120661 ttttgaattt ggcgatgagt tgctaagaac attgttcagc catcaggaag aatttcaaga
   120721 tttttccgaa ttcaaaacag aactttattt gaaaacagcg agaaacttta tgcgctatcg
   120781 ctggatgatc acttatttat tcggtgcttc accaatgagt gagaagaact actttttaga
   120841 tgaatcacat ccgcaagaac ctgttcgcag tattcgcaac agtgcgctgg gctatacgaa
   120901 tcatccaaat gtgaaagttt cctatgcttc tatgaaacag tatttagcag atatcgagcg
   120961 aatgatcgaa gaagggaaac tttcggagga gaaagaattt tatacaccgc ttcgtttccg
   121021 aggcggaaag aaagtcgcag atttagcaac aacaggtgtt cgttatattg aattgcggaa
   121081 tatcgatttg aatccttatg caagattggg tatcaatcca gagcaagttc ggttcttaca
   121141 attattcctg atgtacatgt tatggacaga agaaaaagaa gactgtgacc aatgggtagc
   121201 agaaggtacg actcgaaata acaaagtagc actagaacaa ccatcagatc aaacagaatt
   121261 tcatcaagaa ggtagagaaa ttcttgaagg aatgaagcaa atgctggttg aattggattg
   121321 gttggattct ctttatttag tagaggaagc attgactcaa atggatcatc ctgaacaaac
   121381 gttagcagcg aaactctatc aagaagcaca gctatcgagt cagcaagaag ttgctgtggc
   121441 attgggacat caatattata aagaaagcca tgaacgtccc tatcagttgg ccggttttcg
   121501 cgaaatggaa ctttctactc agatatttat gttcgatgcc atccaaaaag gtgtccaagt
   121561 caaagtatta gatgagtcgg atcaattttt gcgtctgcaa ttccaagacc atgtagaata
   121621 tgtgaaaaat gcaaacatga caagtaagga cagctatatc gtcccactta tcatggaaaa
   121681 caaaacagta acgaaaaaag tcttaaaaga agctgggttc cgggtaccag gaggcgcaga
   121741 attttcatcc atggaagaag cggtaaaagc ttatccgaga tttgccgagc aggcatttgt
   121801 gatcaaacct aaatcaacca attacgggtt aggtatcacg attttcaagg aaggagctag
   121861 tctggaagat tatcaagcag gtttagccat tgcttttcgt gaagacagtt cggttttagt
   121921 agaagaattt atgccgggaa cggaatatcg tttctttgtg atcgacggag aagtccaagc
   121981 catcatgttg cgagtccctg ctaatgttat tggtgacagt atccgaacag tgaaagaact
   122041 tgttgaagaa aaaaatagtg accctttgcg tggaacgaat catcgtgcac ctttagaatt
   122101 gatccaatta ggtgaattag aacaattgat gttgaaagaa caagggttga cgatcgaatc
   122161 agttccccaa gccaatcaaa ttgtttatct aagggagaac tcaaatatca gtacaggtgg
   122221 tgattcgatc gatatgactg atgaattttc tgaagcttac aaaaaaatcg ccgtttctgc
   122281 ggtagaagca ctaggagcta agattagtgg tatcgatttg attataccag ataaagaaat
   122341 cgatcctaca actgataaaa aagcatatgg gatcatcgaa gcaaatttca atccagccat
   122401 gcatatgcat gtttatcctt ttgcaggaaa aggcagaaga ttgacgatga atgtattgaa
   122461 attgttatat cctgaagtat tttaaaagaa aaagcttttg acagattgtc ctatttgatg
   122521 tcaatctgat caaaagcttt ttttgcatgt aaaaatttat gtgttttttc gttttaaaat
   122581 aaacgatccc agaagctttt tttctttttt ggtttttctt tttcagctgg cgaagagttt
   122641 tcttctgggt attttttttc gatatcaatc acaggttcat ttactgcttc ttttgctgtt
   122701 aagagcaatc cgaattcctc aggatttgca gtgacgtaat cgttgactac agtgaaacgg
   122761 ctattgtttt tcgttgctag ctggatatat tggttttgga cagaaagggg tagagcccca
   122821 ttcaagagta tggaagcatc tggatgtttt ttgagttctt ttaaaaagtt ttctttgttt
   122881 tttggttgga tcatttcagc aacagtcata ctaagatagc agcgttccct gaatgtccct
   122941 aaatatttat gctgttcttc agggttcact aaaggtgtac cataaagtcc cttatccaaa
   123001 tggcttttta catcatcttg tgccataaaa aataccctcc ttcttctctt tgtctttctt
   123061 acgattatag catattccca ataataccta aaacatatga aaaagcggtt gcttttgttc
   123121 aacgattcac ttaaaataag aatgaaaatc attctcaatt agaaaggtgg attggatgcg
   123181 ctacggacga ctagtagcat tattagttat tttaagtagt atttcattgt tcatcggagt
   123241 atcgaatgta caattgtcag aactgctcca gttagatggg caaagcttac aaatcttgct
   123301 agtgactcgt ttgccacgaa cgatttgttt gatccttgtg ggagcgacca gtagtatttg
   123361 tgggttgatc atgcaacatt tgacccaaaa taaatttgtt tcaccaacga ctgcgggcac
   123421 gatggatagt gcgcgtttag gtattttagt ggctatgatt ttcctacctg gtgcatcctt
   123481 gattgtccga tccttgacag cattttgctt tgcttttgca ggaacattgc tttttttatc
   123541 ccttacgcga ttgttcctcc agaaaaatca attgatgctt ccgttgattg gagtcatgtt
   123601 agggaatatc attggttcag ttgctacttt ttttgcttat caattccagt tggtacaaaa
   123661 tatgacttca tggcttcaag ggaattttgc gactgtgata cgaggaaatt atgagttatt
   123721 gtatgctgtt gttccattgt tgctcttggt ttggctattt gcttaccgat ttacgatcat
   123781 tggtctgggc gaatcgtttg cagtaaatct gggaatagat taccagaaga tgcagttttt
   123841 aggcattggc ttagtatcat tggctagtgc agtcatgttg atcatggtag gaagcattcc
   123901 ttttatcgga gtgatcattc cgaatcttgt ctctagaata tatggagatc aagtgcacaa
   123961 aacaattggc gtaactgctt tagcaggtag tttgttctta gtattttgcg atcttgtcgc
   124021 tcgcgtgttg atttttcctt atgaaatacc agtcagtgtg atcgttggaa tcatcggtgg
   124081 ggctatcttc ctttatcttc tgataagggg acgacgatat gcttaaaaaa aagccatcta
   124141 tttttattgg tatgcttgtt cttgttttat ttgttacagg aatgtacttg acgatccata
   124201 catatggaaa ctggcatttt gcatgtgcat tgaggagtaa gaaaattttt gcatttatcc
   124261 ttgtctcgct agctgtcagc agtgccacaa tcagctttca gacaattacg cataaccagt
   124321 ttttgactcc ttcagtattg ggacttgagt cgttatatgt ccttgtccaa acggtccttt
   124381 tcttctttat cggaggagtg acgatgcttc agcagacagg tacagttttt ttcttattga
   124441 acgttgggct gatggtcggg ctgagtctat tgttattttt ctttcttttg aaaaaaatga
   124501 gagcgaatct ttttttatta ctgatgtcag ggatgatttt aggtacgctt tttggtagtg
   124561 tcagtacctt tttacaagtc atcatggatc cgaatgaata tgatttgctt caaggcagat
   124621 tatttgcaag ctttggcaat atccagagtc agtatctttt gcctgcttct ttgatcatta
   124681 tcggctgctg gtacctgtta tttcgatatc gacatgtgct agacgttttc catctcggaa
   124741 atgaacaagc agtaaacctc gggatagaca cagatcgttt tgaatttttg attttatgtt
   124801 gtgtcagtct tttgacaggg acatcaacgg ctcttgtggg tccagttact tttttagggt
   124861 ttattgtcgc aaatatcagt tatcgtgtct tttctactta tcgtcatggt cagctattta
   124921 tcggaagctt tctggtagct atgctgtttc tgacaggagg acagctcgca gtagagcaga
   124981 ttttcggctg gaatacaaca gtgagtgtcg tcgttgaatt tgtcggagga atttattttg
   125041 tcacaaaatt gattcgcgaa aggaagagta tcctatgatt gatttacaaa aggtcaataa
   125101 actgtaccat ggaaaatcag tggttaaaga tgtacagtta acgattcctg aaaagaagtt
   125161 gactgctttt atcggtccaa atggtgctgg aaagtcaacg gtgttgtcga tgatcagtcg
   125221 tttgatacca aaagatacag gtgaaatcta tttagaccat aacgaagtaa aggcttggaa
   125281 atccaatgaa ttagccaaaa aattatcgat cctacgacaa tcgaatgaaa taaatgtcca
   125341 aatcaccgtg aaagaattag tagaatttgg acgatttcct tatacgaaag gaaaattgac
   125401 gacaaaagac gaggaaatcg ttcaggaatc actggaacac attgggttag aagaaatagc
   125461 ggatcaatcg attgacactc tttcaggcgg tcaactccaa cgtgcgtata ttgcaatggt
   125521 gctagcgcaa gacacggatt atattttact ggatgaacct ttgaacaatc tagatatgaa
   125581 ttttgcggtt caaatcatgc agattttaaa aaatttagta caagaaatcg ggaaaacaat
   125641 tgttattgtt cttcatgata tcaactttgc tgcaagttat gcagacgaaa ttgttgcaat
   125701 gaaagatgga cagttattta cgcaaggaac gacccaagaa atgataaaaa aagatgtatt
   125761 ggatgaatta tacgaaatgg acatacggat ttgtgagatt gaaggccgca gattctgtct
   125821 ttatttctaa aactaacatt aggagagaag agatgaaaaa attagtagta ggtatcttga
   125881 ttctagcatc atttggttta gcagcatgtg gaaatagcaa caacaccagt caggcagaca
   125941 caaaaaaaag cagcacacaa actgaaacta cattgacgat aacagatagt aatggagacc
   126001 aaatcgaggt gccaaataat ccagaaaaag tcgtcgtatt cgacaatggt tctttagata
   126061 cgatggatgc tcttggtgtt ggagataagg tagttggagc agctacaagc agccttcctg
   126121 agtatctttc atcctataaa aaagtagaat cagcaggtgg aataaaagaa ccagacttag
   126181 aaaaaattaa tcagcttcag ccagatctaa tcatcatttc tggccgacaa agagattttc
   126241 aaaaagatct aagtcaaatt gctccgacca ttttcctttc tttagatgcg aaaaatccat
   126301 gggaatcttt ccagcaaaat gtcactgctt taggggagat ctttggtaag caagaagaag
   126361 ctaaaactca attagaggaa ttatctagtg caatcgatca gacgaaaaag aaagcagaag
   126421 ctactgacaa aaaagcacta gtcacacttg tgaatgaagg gcaattatca gcctacggat
   126481 ctggttctag attcggtttc atccatgatt tatttggctt tgaacaagca gatgatcaaa
   126541 tcgaagcatc cactcacggt caaagtgttt cttatgaata tgtgctggaa aaaaatccgg
   126601 atatcttatt tgtagtagac agaacaaaag ctattggtgg agacgacagc aaagatgata
   126661 tttctgccaa tgaactggta gcacaaacga atgctggtaa aaatcagcag atcatctccc
   126721 ttgaaccaga tgtctggtat ttaagcgggg gcggtttaga atcaatgaaa ctgatgatcg
   126781 aagatgtgaa tcaagcattt aaatagcata aataacagag gattaaactc tcatactatg
   126841 taacaataat gtttcttaca ttcttaaata gcaagagcct atcctcaaag aagttatcaa
   126901 aaaccatccg taaaatcgta ttgatgattt tacggatggt ttttatttat ttgagaaatc
   126961 ttttttcaac gtgaattcat tatctacatc atacgggttg attctagaat ggggagtggc
   127021 aaaaaatagt gtgatccatt ttttcagcat caagatcgcc tcctttgatt tgaatcaaag
   127081 gtacaaagcg aaacttagat aaaacttaaa tttagatatt tttattttgt accgatctct
   127141 tctttaaacg atgttccatc tgctgcttct cggcaataag tccactagac tagaaaatat
   127201 gagaagctat tttctgtcca gcgttcttat tactcaaaac aaatcgcagt tgtcacaatc
   127261 tcttctaaac gatgttccat ctgctgcttc tcggcaataa gcccactaga ctagaaaata
   127321 tgagaagcta ttttctgtcc agcgttctta ttactcaaag caaatcgcag ttgtcacaat
   127381 ctcttctaaa caatgttcaa tcagctgacc ttcggtatta gctcaaagca ctgatttcac
   127441 aacctctatt taggcatgtg tctcagcaag cggttgatag ttgatgatct gcatttttcg
   127501 ataaaaatcg gcgggaagct tactccaccg ttttagtaat tgatagaaat aactctcttc
   127561 tgctttgatt ttatactcgc ttaatatcaa tcca
//
LOCUS       DACDRN010000003       115929 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDRN010000003 DACDRN010000000
VERSION     DACDRN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 115929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 115929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..115929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.003"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(606..962)
                     /locus_tag="IWJ80_000286"
     CDS             complement(606..962)
                     /locus_tag="IWJ80_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP9460056.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(976..1386)
                     /locus_tag="IWJ80_000287"
     CDS             complement(976..1386)
                     /locus_tag="IWJ80_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9460057.1"
                     /translation="MIKVYTSPSCTSCRKAKAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1732..3918)
                     /locus_tag="IWJ80_000288"
     CDS             complement(1732..3918)
                     /locus_tag="IWJ80_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9460058.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            4136..4819
                     /locus_tag="IWJ80_000289"
     CDS             4136..4819
                     /locus_tag="IWJ80_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP9460059.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4900..5619)
                     /locus_tag="IWJ80_000290"
     CDS             complement(4900..5619)
                     /locus_tag="IWJ80_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9460060.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(5931..6137)
                     /locus_tag="IWJ80_000291"
     CDS             complement(5931..6137)
                     /locus_tag="IWJ80_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460061.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6382..6984
                     /locus_tag="IWJ80_000292"
     CDS             6382..6984
                     /locus_tag="IWJ80_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP9460062.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7125..8405
                     /locus_tag="IWJ80_000293"
     CDS             7125..8405
                     /locus_tag="IWJ80_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9460063.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8418..9146
                     /locus_tag="IWJ80_000294"
     CDS             8418..9146
                     /locus_tag="IWJ80_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9460064.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(9215..10738)
                     /locus_tag="IWJ80_000295"
     CDS             complement(9215..10738)
                     /locus_tag="IWJ80_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9460065.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            10990..12171
                     /locus_tag="IWJ80_000296"
     CDS             10990..12171
                     /locus_tag="IWJ80_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9460066.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(12231..13859)
                     /gene="treC"
                     /locus_tag="IWJ80_000297"
     CDS             complement(12231..13859)
                     /gene="treC"
                     /locus_tag="IWJ80_000297"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP9460067.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(14310..17021)
                     /locus_tag="IWJ80_000298"
     CDS             complement(14310..17021)
                     /locus_tag="IWJ80_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9460068.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(17031..17732)
                     /locus_tag="IWJ80_000299"
     CDS             complement(17031..17732)
                     /locus_tag="IWJ80_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9460069.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            17918..18517
                     /locus_tag="IWJ80_000300"
     CDS             17918..18517
                     /locus_tag="IWJ80_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9460070.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            18598..19611
                     /locus_tag="IWJ80_000301"
     CDS             18598..19611
                     /locus_tag="IWJ80_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9460071.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            19719..20636
                     /gene="rbsK"
                     /locus_tag="IWJ80_000302"
     CDS             19719..20636
                     /gene="rbsK"
                     /locus_tag="IWJ80_000302"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP9460072.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            20664..21059
                     /gene="rbsD"
                     /locus_tag="IWJ80_000303"
     CDS             20664..21059
                     /gene="rbsD"
                     /locus_tag="IWJ80_000303"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP9460073.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            21059..22432
                     /locus_tag="IWJ80_000304"
     CDS             21059..22432
                     /locus_tag="IWJ80_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9460074.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            22789..24933
                     /locus_tag="IWJ80_000305"
     CDS             22789..24933
                     /locus_tag="IWJ80_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9460075.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFNHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(24985..26025)
                     /locus_tag="IWJ80_000306"
     CDS             complement(24985..26025)
                     /locus_tag="IWJ80_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9460076.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(26302..28104)
                     /locus_tag="IWJ80_000307"
     CDS             complement(26302..28104)
                     /locus_tag="IWJ80_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9460077.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(28118..28870)
                     /locus_tag="IWJ80_000308"
     CDS             complement(28118..28870)
                     /locus_tag="IWJ80_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9460078.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(28879..29241)
                     /locus_tag="IWJ80_000309"
     CDS             complement(28879..29241)
                     /locus_tag="IWJ80_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9460079.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(29417..30289)
                     /locus_tag="IWJ80_000310"
     CDS             complement(29417..30289)
                     /locus_tag="IWJ80_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP9460080.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(30340..30828)
                     /locus_tag="IWJ80_000311"
     CDS             complement(30340..30828)
                     /locus_tag="IWJ80_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP9460081.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(30966..31796)
                     /locus_tag="IWJ80_000312"
     CDS             complement(30966..31796)
                     /locus_tag="IWJ80_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP9460082.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(31812..32420)
                     /locus_tag="IWJ80_000313"
     CDS             complement(31812..32420)
                     /locus_tag="IWJ80_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP9460083.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(32413..32730)
                     /locus_tag="IWJ80_000314"
     CDS             complement(32413..32730)
                     /locus_tag="IWJ80_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9460084.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(32886..33614)
                     /locus_tag="IWJ80_000315"
     CDS             complement(32886..33614)
                     /locus_tag="IWJ80_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP9460085.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            33773..34654
                     /locus_tag="IWJ80_000316"
     CDS             33773..34654
                     /locus_tag="IWJ80_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9460086.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(34891..35934)
                     /locus_tag="IWJ80_000317"
     CDS             complement(34891..35934)
                     /locus_tag="IWJ80_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9460087.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(35937..36605)
                     /locus_tag="IWJ80_000318"
     CDS             complement(35937..36605)
                     /locus_tag="IWJ80_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9460088.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            36747..39083
                     /locus_tag="IWJ80_000319"
     CDS             36747..39083
                     /locus_tag="IWJ80_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9460089.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(39198..39509)
                     /locus_tag="IWJ80_000320"
     CDS             complement(39198..39509)
                     /locus_tag="IWJ80_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460090.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            39690..41165
                     /locus_tag="IWJ80_000321"
     CDS             39690..41165
                     /locus_tag="IWJ80_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP9460091.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            41212..41796
                     /locus_tag="IWJ80_000322"
     CDS             41212..41796
                     /locus_tag="IWJ80_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP9460092.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            41798..42217
                     /locus_tag="IWJ80_000323"
     CDS             41798..42217
                     /locus_tag="IWJ80_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP9460093.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(42498..42782)
                     /locus_tag="IWJ80_000324"
     CDS             complement(42498..42782)
                     /locus_tag="IWJ80_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP9460094.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(43134..43844)
                     /locus_tag="IWJ80_000325"
     CDS             complement(43134..43844)
                     /locus_tag="IWJ80_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP9460095.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(43841..45313)
                     /locus_tag="IWJ80_000326"
     CDS             complement(43841..45313)
                     /locus_tag="IWJ80_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9460096.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            45451..45924
                     /locus_tag="IWJ80_000327"
     CDS             45451..45924
                     /locus_tag="IWJ80_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9460097.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(46077..46628)
                     /locus_tag="IWJ80_000328"
     CDS             complement(46077..46628)
                     /locus_tag="IWJ80_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9460098.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            46733..47263
                     /locus_tag="IWJ80_000329"
     CDS             46733..47263
                     /locus_tag="IWJ80_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP9460099.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(47746..48189)
                     /locus_tag="IWJ80_000330"
     CDS             complement(47746..48189)
                     /locus_tag="IWJ80_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9460100.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(48205..49365)
                     /locus_tag="IWJ80_000331"
     CDS             complement(48205..49365)
                     /locus_tag="IWJ80_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9460101.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            49872..51572
                     /locus_tag="IWJ80_000332"
     CDS             49872..51572
                     /locus_tag="IWJ80_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460102.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(51674..52507)
                     /locus_tag="IWJ80_000333"
     CDS             complement(51674..52507)
                     /locus_tag="IWJ80_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP9460103.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(52811..54976)
                     /locus_tag="IWJ80_000334"
     CDS             complement(52811..54976)
                     /locus_tag="IWJ80_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9460104.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTCFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            55236..57530
                     /locus_tag="IWJ80_000335"
     CDS             55236..57530
                     /locus_tag="IWJ80_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9460105.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            57523..58188
                     /gene="pgmB"
                     /locus_tag="IWJ80_000336"
     CDS             57523..58188
                     /gene="pgmB"
                     /locus_tag="IWJ80_000336"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9460106.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            58181..59188
                     /locus_tag="IWJ80_000337"
     CDS             58181..59188
                     /locus_tag="IWJ80_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9460107.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            59210..60229
                     /locus_tag="IWJ80_000338"
     CDS             59210..60229
                     /locus_tag="IWJ80_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9460108.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(60510..61877)
                     /gene="nhaC"
                     /locus_tag="IWJ80_000339"
     CDS             complement(60510..61877)
                     /gene="nhaC"
                     /locus_tag="IWJ80_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9460109.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(61986..63407)
                     /locus_tag="IWJ80_000340"
     CDS             complement(61986..63407)
                     /locus_tag="IWJ80_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9460110.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(63650..65527)
                     /gene="tdc"
                     /locus_tag="IWJ80_000341"
     CDS             complement(63650..65527)
                     /gene="tdc"
                     /locus_tag="IWJ80_000341"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9460111.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRNVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(65818..67074)
                     /locus_tag="IWJ80_000342"
     CDS             complement(65818..67074)
                     /locus_tag="IWJ80_000342"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP9460112.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            67654..68955
                     /locus_tag="IWJ80_000343"
     CDS             67654..68955
                     /locus_tag="IWJ80_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9460113.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(69024..69623)
                     /locus_tag="IWJ80_000344"
     CDS             complement(69024..69623)
                     /locus_tag="IWJ80_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9460114.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            69877..70758
                     /locus_tag="IWJ80_000345"
     CDS             69877..70758
                     /locus_tag="IWJ80_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9460115.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            70771..72381
                     /locus_tag="IWJ80_000346"
     CDS             70771..72381
                     /locus_tag="IWJ80_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP9460116.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(72458..73117)
                     /locus_tag="IWJ80_000347"
     CDS             complement(72458..73117)
                     /locus_tag="IWJ80_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9460117.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(73400..74065)
                     /locus_tag="IWJ80_000348"
     CDS             complement(73400..74065)
                     /locus_tag="IWJ80_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460118.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(74212..74817)
                     /gene="dhaL"
                     /locus_tag="IWJ80_000349"
     CDS             complement(74212..74817)
                     /gene="dhaL"
                     /locus_tag="IWJ80_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP9460119.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(74845..75828)
                     /gene="dhaK"
                     /locus_tag="IWJ80_000350"
     CDS             complement(74845..75828)
                     /gene="dhaK"
                     /locus_tag="IWJ80_000350"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP9460120.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(75830..76225)
                     /gene="dhaM"
                     /locus_tag="IWJ80_000351"
     CDS             complement(75830..76225)
                     /gene="dhaM"
                     /locus_tag="IWJ80_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP9460121.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            76379..76699
                     /locus_tag="IWJ80_000352"
     CDS             76379..76699
                     /locus_tag="IWJ80_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP9460122.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(76811..77680)
                     /locus_tag="IWJ80_000353"
     CDS             complement(76811..77680)
                     /locus_tag="IWJ80_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9460123.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(77776..79080)
                     /locus_tag="IWJ80_000354"
     CDS             complement(77776..79080)
                     /locus_tag="IWJ80_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9460124.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIANAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(79077..79586)
                     /locus_tag="IWJ80_000355"
     CDS             complement(79077..79586)
                     /locus_tag="IWJ80_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460125.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(79761..81896)
                     /locus_tag="IWJ80_000356"
     CDS             complement(79761..81896)
                     /locus_tag="IWJ80_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9460126.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(81979..83634)
                     /locus_tag="IWJ80_000357"
     CDS             complement(81979..83634)
                     /locus_tag="IWJ80_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9460127.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(83636..83950)
                     /locus_tag="IWJ80_000358"
     CDS             complement(83636..83950)
                     /locus_tag="IWJ80_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9460128.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            84120..84380
                     /locus_tag="IWJ80_000359"
     CDS             84120..84380
                     /locus_tag="IWJ80_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP9460129.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            84394..84708
                     /locus_tag="IWJ80_000360"
     CDS             84394..84708
                     /locus_tag="IWJ80_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP9460130.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(85438..85992)
                     /locus_tag="IWJ80_000361"
     CDS             complement(85438..85992)
                     /locus_tag="IWJ80_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP9460131.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(86118..88047)
                     /locus_tag="IWJ80_000362"
                     /pseudo
     CDS             complement(86118..88047)
                     /locus_tag="IWJ80_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(88353..89069)
                     /locus_tag="IWJ80_000363"
     CDS             complement(88353..89069)
                     /locus_tag="IWJ80_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9460132.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(89070..90893)
                     /gene="glpO"
                     /locus_tag="IWJ80_000364"
     CDS             complement(89070..90893)
                     /gene="glpO"
                     /locus_tag="IWJ80_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP9460133.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(90907..92403)
                     /gene="glpK"
                     /locus_tag="IWJ80_000365"
     CDS             complement(90907..92403)
                     /gene="glpK"
                     /locus_tag="IWJ80_000365"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP9460134.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(92539..93999)
                     /locus_tag="IWJ80_000366"
     CDS             complement(92539..93999)
                     /locus_tag="IWJ80_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9460135.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(93989..95284)
                     /locus_tag="IWJ80_000367"
     CDS             complement(93989..95284)
                     /locus_tag="IWJ80_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9460136.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(95616..96293)
                     /locus_tag="IWJ80_000368"
     CDS             complement(95616..96293)
                     /locus_tag="IWJ80_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP9460137.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(96323..97414)
                     /locus_tag="IWJ80_000369"
     CDS             complement(96323..97414)
                     /locus_tag="IWJ80_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP9460138.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(97515..97949)
                     /locus_tag="IWJ80_000370"
     CDS             complement(97515..97949)
                     /locus_tag="IWJ80_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9460139.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(98533..99990)
                     /gene="ascB"
                     /locus_tag="IWJ80_000371"
     CDS             complement(98533..99990)
                     /gene="ascB"
                     /locus_tag="IWJ80_000371"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9460140.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(99987..101882)
                     /locus_tag="IWJ80_000372"
     CDS             complement(99987..101882)
                     /locus_tag="IWJ80_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9460141.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(101940..103715)
                     /locus_tag="IWJ80_000373"
     CDS             complement(101940..103715)
                     /locus_tag="IWJ80_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9460142.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(103765..105261)
                     /locus_tag="IWJ80_000374"
     CDS             complement(103765..105261)
                     /locus_tag="IWJ80_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9460143.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(105455..107077)
                     /locus_tag="IWJ80_000375"
     CDS             complement(105455..107077)
                     /locus_tag="IWJ80_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9460144.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(107405..108718)
                     /locus_tag="IWJ80_000376"
     CDS             complement(107405..108718)
                     /locus_tag="IWJ80_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9460145.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(108963..109187)
                     /locus_tag="IWJ80_000377"
     CDS             complement(108963..109187)
                     /locus_tag="IWJ80_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460146.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(109207..109575)
                     /locus_tag="IWJ80_000378"
     CDS             complement(109207..109575)
                     /locus_tag="IWJ80_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9460147.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(109771..110481)
                     /locus_tag="IWJ80_000379"
     CDS             complement(109771..110481)
                     /locus_tag="IWJ80_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460148.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(110499..110996)
                     /locus_tag="IWJ80_000380"
     CDS             complement(110499..110996)
                     /locus_tag="IWJ80_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9460149.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(111033..111485)
                     /locus_tag="IWJ80_000381"
     CDS             complement(111033..111485)
                     /locus_tag="IWJ80_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9460150.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(111620..112225)
                     /locus_tag="IWJ80_000382"
     CDS             complement(111620..112225)
                     /locus_tag="IWJ80_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9460151.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(112675..113295)
                     /locus_tag="IWJ80_000383"
     CDS             complement(112675..113295)
                     /locus_tag="IWJ80_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9460152.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(113297..114166)
                     /locus_tag="IWJ80_000384"
     CDS             complement(113297..114166)
                     /locus_tag="IWJ80_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP9460153.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(114189..114596)
                     /locus_tag="IWJ80_000385"
     CDS             complement(114189..114596)
                     /locus_tag="IWJ80_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9460154.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(114615..115904)
                     /locus_tag="IWJ80_000386"
     CDS             complement(114615..115904)
                     /locus_tag="IWJ80_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9460155.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        33912 a        23949 c        20253 g        37815 t
ORIGIN      
        1 tgtttgttgt cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc
       61 agttttcaaa ggtctacttc atcaggagtg ttccccgatt cgtttttggc tgccgtagca
      121 acttttatat catatcagag agacaagtaa ttgtcaactc ttttttaaaa gtttttttga
      181 tttcgttttt cgaaatctta cagcaactca tttatcataa cttatcattt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc acaagtggtt cttttttaac
      301 aactcagtta tcttatcatg tcatttctaa catgtcaaca actattttat atgtttttta
      361 aaatactatt ttattgcttt gctacttaaa acaacagaaa tgaatatatc atgtaaattt
      421 acacatgtca atatattttt aactctatat ccccacctag atttgaattt tgaccacata
      481 aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat aacacgcttg
      541 atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat taaaacagtt
      601 aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc aataaataga
      661 acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt gcataaaaaa
      721 atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa agcctcagac
      781 caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc ttctgcttct
      841 tctgtactta gatcgcgttc ttctaagtca acaaattgat aaggcaattg atggtgttct
      901 aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac tttcacttcc
      961 acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga tcagatttct
     1021 ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat taaatccgat
     1081 tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat attctttgaa
     1141 taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct tttcgtaaat
     1201 tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca agatcttttt
     1261 gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt caagttgatt
     1321 cgcttgcaac catgcttttg cttttctaca ggaagtacag ctaggcgaag tatatacttt
     1381 tatcattttc tcactccttt ttaattataa tccaattcta cagtaatttc ttttaaaata
     1441 ccaattatta gctaataaaa tataaaaaaa atcaaaataa ctgattattt acgtttgaat
     1501 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1561 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1621 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1681 attccttctt ctttcttgaa actgaccact tctgtcacac cctctcttct atcactttcc
     1741 ttttttcaca cgactcccgg tttctttgat aaatctcgat actttcaatt ttcttgaatt
     1801 tttcatttga ggtacacata gatacagacg atttcttgct ctagtcatcg caacataaaa
     1861 tagtcttcgt tcttcttcta tcgtcgttac tggttctgct tttatttcat taaagtcgat
     1921 tactttcttt tctgtcgctc cgttataagg caaacttcct tcgttacaac caatcaaaaa
     1981 gacatttcgg taactaagac ctttactttt gtgaatgctc tggatacata gtgcatcaag
     2041 ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct ttagctgtcg cttgggtttc
     2101 ttgcgataag gcttggagtc ttctgaaagt agcatgctgc tttacttctt ctcgtatcca
     2161 ttcttgatca ctctggtcga tttccttagc tatacgtggc agatgaagat agctttgaac
     2221 aagcgaatag atttcttgat gctggtctgt tttacgcaac gcttcctcaa actctcggat
     2281 ttctgcagat ttcgatttta aattggggct tatcgacaaa atataatctg caacggatat
     2341 attttgatat ttaggagaaa agtattgatc gcattcactt tttttcaggt agcagtctgc
     2401 catcagctgc cggagcaatt gctggtaggc ctgatttttc ttttgcagat tcagtgctgt
     2461 ttgccaatga acaatttttt gatagaaatg ttggaaagac ttgataccaa agataccttc
     2521 atatggacta tctgcttcca gtacaaaagg aatattcgtt ttcagcagag attggacaag
     2581 ctgctgcgtt tgtgaagctg atcggtataa tatgattgat tccttatatt tcccattttt
     2641 ttgaataaat gtctgaattt gtttgattag ccactgactt tctgctttag tatcagaaaa
     2701 caaataggct tccataacac ctgcttcttt ttgtgctgga atcatcgcct ttggtatccg
     2761 atcattttta ttatagctga tcaatctatt tgctccttgg acaatagctg cccgagaacg
     2821 ataattagtt aataagaaaa gagttttggt aggatattct ttttcaaagt tcttgatgta
     2881 tgctggctct gatcctctga atccatagat actctgatca tcatctccaa caacaatcaa
     2941 cttgatcctg ttttcctttg tcatccgttt cacgatttcc cattgaagtg gattagtatc
     3001 ttgaaattca tcaatgaata gataatcata tttttggccg atataatgct ggatctcctc
     3061 attctccaaa gcgcttttca gattttggag tacatcattg aactctagca attgctggct
     3121 tcttttttgt tcttggagtt ccgtgagcaa tccatacagt cgttctgata tcggctgtcg
     3181 tgcattctta tcaatggaat catatttttc tttaactaac tgaaccattt cttctactga
     3241 aaggttcata ttctgccaat agtctaaccg agaagcaatc gttttttctc catattgatc
     3301 aaatatataa agattatatt tacgtatcag ctgttgcaaa acagcattca ctttttgtgc
     3361 agtatccttt gaaaagtctc cttgtattcc ttgcattgct acttcaggaa attttcgaca
     3421 aatttcattg tatatcctaa aaaagatact atggaatgtt ccgatatgga cagcggtttt
     3481 tctctggctg aaattatcta ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt
     3541 gatcattagt atgcgttctt cgtctacttg tttttgcgtc atcagataaa cagcttttgc
     3601 acagattgtt tgcgttttgc cgctcccagc tcctgctatg attcgaagat gtttcttcat
     3661 atcatatgtg atagctgctt tttgttcttg attgaagcga ttataggaac gtttctccat
     3721 cattttaaag aaagagctgt ttccttcact ttcttttact tgtacttctt cctcaataag
     3781 tccgggtgct tcgccataag ccgcttcata ggtagcaatg atttcttctt gtagccgaaa
     3841 gacttgttga agctttttat acttgttctc ccagcgtttc aattcttttt gtaatcggag
     3901 aatttctttt tcattcaact gattgatttc cttcccatct ttcttctttg aatttgttta
     3961 tttcaagttg gttatttaaa gtttcgttgt ctattttttc tttatactaa cagacctttc
     4021 taaaaatgaa aatgggaagc gatatgattt tagttttctg aaaatttttc ctttttttca
     4081 atcgtttatg ctaagataag gaagacaaaa atattcataa aaggtgatga aaaaaatgca
     4141 gacttccttt cacgaggggc gtacaatagg acttttgctg acatttatcg gaggagccat
     4201 ggacagttat acttatatcc aatatggtgc ttttgcctct gctcaaactg gcaatatcat
     4261 tctatcgatc atccaagcat ttgacggaga atggctaagt gttggtaaaa aagtattgtc
     4321 tacccttttc tttttcttag ggattcttct aacaaagtat ctaattgatt attttagaaa
     4381 aaaagaaaaa catttctggc gcttgtttgt tctatattat gaagcagtga tttttttctt
     4441 tgttagtttg acacccgtcc atgagcatcc cgcattagta acgattttga ttgcttttac
     4501 tgcatcgatc cagtgggtag cttttgataa aatcaacggg cgtgcctaca ctaatttatt
     4561 tacaactgga aatttaaaag gggtagcaac gaacttatat gattattgta tttctaaaga
     4621 aaaagcagat tttgatcgat ttttccacta tctaagtgtc gttcttgcct ttatcgcagg
     4681 agctattgtc cttgttttct gtcatcatct gtttggcgcc cattcgattt tgatcgtttc
     4741 cattttattt ttgatcatgg ccctcactca gacatttcat ttatggcaat tttatcatac
     4801 gaatcggttc actatttgat ctcaatgaat caaaaaaact aagcaagatg ctgctcacag
     4861 agaaatatct gcgagtgagc aatttgctta gtttttatat tatttagttg taaagattcc
     4921 ctttgctttc gggttatttg tagtgtagcg gatcgactgg gcatctgcct cttctgtcat
     4981 tttctttgcc cgcatcttga tttcttgtac gatttgttct ccacatgccg gatcggctac
     5041 gaaatcatat ttgtccccat cgatcactat tttgggacat atatcaaact gttcatacca
     5101 ctcttcgtat cgtcgattga gttctttata gtaggaatag agttcaggat cttgttctag
     5161 ctgttcatat tcccggcctc tttttttgat tctcgctaac atagtttcaa aggatacacg
     5221 aatataaacc agtagatctg gtttcttttg tggcgaaatt tcttctaact ccttcagcat
     5281 cgtttctaaa agaccttcat actgctttac ttctacttca gaaacacgcc ctaaatctgc
     5341 attcaaatga aacagcatac tatcttcgta gatggagcgg tctagtacgt tgtcatcatg
     5401 gctgaaagca tctttgattg ccaaaaagcg tttgttcaaa aagaaaatct gtaataaaaa
     5461 agtatacttt tctggattag aataaaatag cggcaatact tcattatctt ccacgttctc
     5521 ataaaaaggt tttgtttcta aatcttgggc aagcatttca gttagtgtgc ttttccctgc
     5581 accgatcgtt cctgcaacaa tgatcacaat tatcggcatc cttcctgtaa taaaatctgt
     5641 tcttcatttt actacttcat ttatagaaat aaaagccttg actttatctg ctccttaaaa
     5701 gctacttctc taaaagtagc caaaagtaac tcctattcat gaaaataaag gctgtgacaa
     5761 aagtcttgtc gcagcccttt tttcaaataa acagtattct cctgccaaca cctcggtcac
     5821 aaggcaatat tcataaaaca tgaaaagctg ttttttaaca ttattccttg taactcagag
     5881 ctaaacggga gactcacaac ctctttatgc tctcaacatt ttatctgttt ttattcttct
     5941 attgtacttg atttgcgtac aactaactgc cagtgcagat tatgagcggt atcattgtct
     6001 tctactgcgc atttcatgac acgtatcatc acactatttt catagatttt ttctattttt
     6061 ccttttattg gtgattcaaa taaatcagat ttaaatgcat atgtttcgcc gacttcaaac
     6121 aattgttcat tttccataag aatccctcca tcacctttta taaggtcagt aattttttaa
     6181 cataaatcac tcgaaaaagc acaaacttgt cataaaaagt acttttcgta tcaaaactgt
     6241 cacttttttt ggtaaggttc ttggaaaacg aagatttttt taggagatca tgacggtttt
     6301 gatgtcaaaa ccacttttta tgaaacaaaa gacgtttttt atttttttta gcataaaatt
     6361 tgtggtggag gtacgtatga gatgaaagat aaagttgtga acaaattatt agaagcctta
     6421 ttcagcggag acaaatcgga agaagatatg gtgtatgtcc aagtaaaatt tgtcttagaa
     6481 gttttattga ataatcttgg aaaactagcg gtcgttctag tcttttctct ggtgacaggc
     6541 tcatgggcgg aaacaggaat cacctttctt tcttatatct gcatccgcag atatgcttat
     6601 ggattgcatt ccgactctga attcgtttgt ctgctgtgga cactacttta tttatggggc
     6661 gttcccttgg tgatgaaaca tctgcaattg actatctcct tcccaatgat ggtctttttg
     6721 ttaatcagct gctttttgtt actgcttcgc tatggatcaa gaggtacagc aatcaatccc
     6781 attgaacccg agaaacgtcc cccattatta aagaaggcga tcagcatgtt tctaattttt
     6841 tctctcatta cgttattttt ctcagcttct tacttttcta cctatttgct acttgggatt
     6901 gttttagaaa tagctacgtt gcttcctatt acaaactact tgatgaattt tggaggaatc
     6961 tttcatgaaa aaaacaatca ttaaacaatt aactaactta tctgactttt ttgtatctgg
     7021 ctgctgcttt ggtatgagtt ctgaattcaa aatccctgaa aaattaaaaa aataatacgc
     7081 caattgtgct ataatttggt aaaaatcaat gggagctgta ctttatgttt ttatggtcca
     7141 tagcgttgca atatttgatt tacacaattt gtatcttatt gattgaccat cacatccgat
     7201 acaaatatct gtttgcggga gtcattctga tttcttgtat tttgattgga tgggtctatt
     7261 cgattttagg gatttttact gctctaattg ctataggaat gctgatgctt acttttttct
     7321 tcttttcgac ttcgcgtcaa caattggttt acgcgcttcc ttttgggtta ttgacgtcgc
     7381 ttttaggaga tcacttaaca agcatcggcg atttttttgt tttgaaatct tcggttattg
     7441 aaccaggaga ttcattgcag tattttcaca ttgtcttatc cgctttttta tctgctttat
     7501 tagctttttg tttaaaaaaa tcgttagaga cttggtttca cactgtggac cgccgtatga
     7561 tcggtacgat cgggacattg atattattta catactatat cattattttt tatacccgtt
     7621 tttctggtga aacacctgat gtcttagctt tgaatacgag ttttttcatt ctatatctgt
     7681 gtgttggcct gatcatcttg gtttattatt ggaaaatctc taataagaaa ttagctgatc
     7741 agttattaga gcaacaagtc aggatgcaac aggattatat caaagatcta gaaaaaaact
     7801 atcaagactt gagagaattc aaacatgatt accaaaatct gcttttttct cttcacagct
     7861 atatttcaga aggcgactta gaaggattac ggtcttatta tgatcaaacg atattaccta
     7921 cacgccagat gatgaacctt tttccggcca atctaagtat gttggataat atgaaagttc
     7981 ctgagatccg tagtttactg tcattaaaac tcatgatggc acaggaaaaa gggttgatcg
     8041 tccagcttca ttttcctgag ccaatcgaac tggatacgaa acatacggta aaccttgtac
     8101 gtatgcttgg tattatcttg gacaatgcga ttgaaggagc tagtgctgca aagaagaaaa
     8161 aaatcgaatt gaccatactc aagaagaatc attccgtgat gattcgtgtg atcaatacaa
     8221 caacaaatac attaccgtta aaccagttga aacaaaaagg gttctcaaca aaaacaaatc
     8281 caaaaaacga aggccttggc ttgtttatct tggatgaatt gacaaagacc aatccttatc
     8341 ttttccttga aacatcaatt gaaaaccagc gtttttctca aacgatttat atacaaataa
     8401 aataaaggag taatgatatg attcccattt atatttgtga agacgatcca aaacaattag
     8461 aagttttatc aacggtgatc aaaaatagaa tcatgattga aaacttagac agttatgtcc
     8521 atatttctac ctctgatccg gaagaacttc tcaatgcagc caagatcaga acttcttctt
     8581 ttggtattta ctttttagat atcgaactag aagacagtga aatggatggt gtagcgatcg
     8641 gtaaattgat ccgtgaatta gatcctttag gaaaaataat ctatgtgact tcccatacgg
     8701 aaatgtcgat gaaaatctta aaaagcaaca tcgaaccgac tgattatatc gtcaaagaag
     8761 atctttatga tttaaaagaa aatgtcgagc atatcctgag caaaattttc gaagattttc
     8821 aaatgtccca aatccaaaaa gatatcttca aaatagagtt taatgatgag atcaaatttt
     8881 taccgatcaa agacatccaa tatttttcta cttcgctagg agcgccacat aaactggaag
     8941 tccatttgac tcatgcccaa ttacaattct atgagaagat caaagaaatc gagaaaatgc
     9001 atcggaattt tatccgttgt cataaatcct atgttatcaa tacacaaaat gtgcggtcga
     9061 ttaataaaaa aaccagagaa gtcacgttgg caaacggcga gaccatccca gcctctatcc
     9121 gtggattaaa gaaattgatt tcataaaaag aaccgaatag tttggaccga ctaaatgacc
     9181 agccgatcca aacccttcgg ttcttttttg cctatcagtg caaaagcatt tcctgtgcaa
     9241 tttcacgtcc atccattgta tctggataaa atgttggcca attctccatt tctttcagca
     9301 atacttcttg actttcattt cctgcatata aatgaaaatg ttcggagtca gccggtgcaa
     9361 tcgtgtgatc actgaattga atatatctaa aagcgccact ttccttatct tctgcttcga
     9421 atagatacct tacgccacgg tttcctgcct cgtaggtcaa gatttctttc cctacataac
     9481 gataatttga acgtttcgtt gttccattct taaacgtaaa tgccatctct ttctcactga
     9541 tcttgattga agcaatatct gtttgatagc ccttcgtata atattcttta tattcttgtg
     9601 ctgtcttatc ctgattcaat ttttctttat attcaaaaac aggatcaaaa gtcccatctt
     9661 ctacatacgt atctactgtt tgccaagttc ctgcccaatc tgaaagagag cgatcttgta
     9721 catcctcgtc ttcaaaataa ccgttgtaga cgctcttttc ttttttgggg gtcaaagtat
     9781 cttctagcgg gttttctgtc tctgtcgttt ttttcaacgc ttcgaggttt gcttccatga
     9841 tagaaagata gttttctcca ttttctattt gttcattcgt caatccttcc aaaggattca
     9901 atacttctaa agatacacct gcttcgtttg ctaacgttct ggcaatacta tctttggcgt
     9961 tttcttcaaa ataaatatag ttgattccat gttctttaac aaagtgtttc agttcagcaa
    10021 ttctcgccgc agacggttct tcactagaag aaagaccagc aatcgatact tgattcaatc
    10081 catattctaa cgctagatag ttgaatgcag catgttgcgt gacaaaagat ctttgttttg
    10141 cttgcttaaa ggtttcttga taaagctgat ggagtgcttg aagtttctcg gtataggctg
    10201 ctgcattttt tgtccatact tcctgttttt ctgggtaagc ttcaatcagc tgatcacgta
    10261 tgtttgctac ctgcttgatt gctaaacttg gagctagcca tacatgtggg tctagctcat
    10321 gtgtatggcc ttcttccccg tgttcatggt catgatcatg tccttcttca cttcccggaa
    10381 gtaaaagcat accttctgtc gcttttatcg tatggacatt gccttcttgt aatgtttttt
    10441 ggattgcggg gacccatgtt tccatgtttt catcattata aacaaagata tctgtatctt
    10501 ggatctttgc catgtcttta gcagaaggtt cataatcgtg ggaatcagtc cctgctggaa
    10561 tcaataactc tacttctcct tcatccccga cgattgcttt tgtaaaatca tacatgggat
    10621 aaaaggtcgt catcacgctc aatttttctt tactgtcctc ggctttccca cttgccccgc
    10681 aagcagttaa catacctgct aaaaccagca tggccaatat acctatcaat cgtttcattt
    10741 atgacactcc tcttctcatc ttctttcatt tgaatgttcc gattcgcttt tcgcaaaccg
    10801 taatagttac gttttagcaa caggatttaa tttatcatat ggttaaaaga actgtcaagc
    10861 gttttcttat tttaattgat gagaaagttt atatgaatga ttgacgatat ttttgcaatc
    10921 aacctctatc attttccttt tatacccgct tttgtctata atgaagttat agataaagga
    10981 ggaaaaagag tgaaaaaaga aacagataca acgaatcgtt tgatccgctt tttaggagga
    11041 aaaacctctt actatgtttt aggaatagtt atcttacttg cgctgatcat ttattttttc
    11101 catcagatct cgtttatatt caagcctgtc ggaatcatta tcgctactat cttgccccca
    11161 ttattgtttg cactgatctt gtattatctt ttggaaccag tcatcaaacg tttgagcaag
    11221 cacctttcga ggaacttgtc agtcattatc gtttatgtga ttattttatt tttattgggg
    11281 cttggaagca tatggcttat tccttttctt gaagaacagg caaagacatt gatcagtagt
    11341 ttgccagatt tgtttcaaga ttttcaagag agtctccgac aatttttgga gaaaacacct
    11401 tttgccgatt catttaatca atttttcgct tctattgacg acgtgacgtc tgatatagct
    11461 ggatttattg gaaattattg ggaaagtggc gcacaacgga tcagcaacat tttttcaact
    11521 gttacagcta tttttattac tttatttact ggtccaattg tcgctttttt ccttttgaga
    11581 gatcctcaaa aattttatca gtcagtttta gcgattgtcc caccagcatt tcgtacagat
    11641 tttaaaaatt tgacaaagat tgctaatcaa caattgggtt catttctaaa ggggcaaatc
    11701 attgcttcat ttatccttgg agcagtctac tgggtttgtt tcttactgat cggattagaa
    11761 tttgctagtg ttttagctat ttctgctggg ttgttatgta tcattccata tattgggccg
    11821 tttatcgcct tctttccagg tttgatcatt gcattccaag actctacttt tatggctgtc
    11881 aaatttgtca ttgtctggtt tgccgtacaa cttctccacg gtgacttggt gattcctcga
    11941 gtaatgggtg atcgtttaca gatccatccg attacgatct taatcgtttt acttgtcatg
    12001 ggtgatctaa tgggaattgt gggcgttatt tttggtattc ctatctatac gttagtcaaa
    12061 ttactcgtta cttttatttt ccgtaaattc aaacaacgct acaataaatt ttatggcgat
    12121 aaaggagagt atgaaaactc ttcattttct gaagaagatt attttaaata aaacacaaaa
    12181 cgtcgatctt acgagaatag ctcgtaggaa cgacgttttg tgtttttgtt ttaatttttc
    12241 agcaaagcaa ttgcctggta tggttttagc tccagacttt gatgaagaac agtatcggaa
    12301 taattatcga taagaatatt ggcttctgtg aatccttctg ggatggcgat ccttgtatct
    12361 ttatcataga aatttgttaa tacaagcagc tgctgtcctt caaattggcg aagaaagcca
    12421 taaacttccg gatgatttgc ttcataagca tgataatctc cttgtgcaac gattggatat
    12481 gtttttctca gccgaatcaa tttcttgtaa taggacaaaa tagaatcttc agaagatagt
    12541 tctttttcta cattaatctc ttctagcttg cctccaactt ttaaccaagg tttccctgta
    12601 gtaaatcctg cttgttctgt gtctgaccat tgcatgggtg ttcgagagtt atcgcgagat
    12661 tttgctttga tccgacgaaa cgcttctttt tctgtgaatc cttgttcaag gagtaactta
    12721 taagcattca aactttctac gtcaatatag tcggaaatcg atgaatagtc tgggtcgatc
    12781 atgccgattt cttctcccat ataaatatag ggcgtcccac ggttcaaatg gatcatggtt
    12841 gctagcatct ctgctccttg ttttcgatac tgatcgatag ctacaaatcg attgagcgca
    12901 cgtggctgat catgattatt aaaaaataga gcattccagc cgttaccctc actcatcttt
    12961 tctccccatg tatgaaatag tcgctttaat tctgcaaaat caaatggagg agtagaccat
    13021 ttattcccat tttcataatc gactttcaaa tgatggaaat taaagaccat tgataattct
    13081 tctctactag gttctgtata caagatacaa ttttcgatcg tcgtagagct catttcaccg
    13141 actgtgatga tagtggaatc cgtcccaaac gttgcttggt tcaattcttt tagatacgta
    13201 tgggtgattg gtttgtccgt atattcaggt ttgccatcat tttctttatt atttttcaat
    13261 tgttcgtctt taccgataac attgataaca tcgaaacgga atccttttac ccctttttcc
    13321 atccaaaaac gaacaacttt gaatagttcc tctcttactg cttggttccg ccagttcaag
    13381 tcagcttgtg tccgatcata taagtgcaaa taatatttcc ccgtatctcc aaatggcgac
    13441 caagcacttc caccaaattt tgatacccaa tccgttggct cttctcttaa aatgaagaag
    13501 tcctgataat gctggtcccc tgctagtgct tttttgaacc attcatgttc tgtcgaaacg
    13561 tgattcagca ccatatcgag catgatttct atccggtatt tctttgcttc ggctattagc
    13621 tgttcgaatt cctccatcgt accaaagata ggatcgatgg ctgtgtaatc agagatatca
    13681 taaccgttgt ctttttgagg gcttggataa atagggttta gccaaatcat gtcgatgcct
    13741 agatcattca gatacgggag tttttctttg attcctttta aatctccaat tccatcacca
    13801 ttactgtctt tgtaagattt gggatagatt tggtaaatga ctttgtcttt aaatgtcatg
    13861 gatatttacc tccattcaac tgatttatta cctaccacaa cttatatata caagttatca
    13921 tttattatta taactgaacc gaaagcgttt gtaaaattta tctttataca ttctaagagg
    13981 tctgttgccc aataaaaaac aagagatcag acatgtattt gtctatcctc ttgtttaaaa
    14041 tagcaaaata ttcttgtttc ttctataaat ggtgttcaaa agcttttatc tacggcaatc
    14101 agtcttctca aatgtaaaac atgaggagct aattttattt gagtattctt tttgctcgat
    14161 gcaggacggc ttttttacaa cctctgattc atggtgttca atcagctgtc cttcggtatt
    14221 agctcaaaaa acggcaaaat atgaaaaact attttgccgt ttgtttctct aatactcagg
    14281 tcaaaccgct gatttcacaa cctctgtttt tattcattcg atttcaacgc atcaatcata
    14341 tcgatatgct ttaatttgaa atgggtaaca atcatcacaa taaccgtaaa tacgatagtt
    14401 aatcctgctg ataagacata agcccaccat gtgatcacta atggaaagat gacattctcc
    14461 atagacgctt gttgcaagat gaagtcagtc aatatatatc ctacaccaaa tcctccgatg
    14521 attcctaaaa gagtaaagat gatattttct cgaacaatgt acatcgtgac ttctttatca
    14581 aaaaatccaa ggacttttat tgtagataat tcgcgtacac gctcggaaat attgatatta
    14641 gttagattat acaagacgat aaaagccagc agtcctgata atacgacaaa gattaagaca
    14701 accgagtcta agttcgccat tgattcttct tgcgtttcga tttgtttgga catgaaagac
    14761 gtatttgtca cagcgtctgt ggcaagcagt ttctcggaaa gcatttcttc ttcttttgtt
    14821 gtcatcttat ctgtacgcag caaaaatgca ttatccatca tcgattgatc agtaactgaa
    14881 gcataatatt caggggacat ataaataaag ctacctagat agtttttcac gatttctttt
    14941 acttcgactt tccattcttt ttgatcttca tcataaagcg aaatatgatc tcctgtagat
    15001 aagtcaaata gttctgctac tttttgcgta atagcgattc ctgttttagg caaggagcgt
    15061 gggttctctt caagatcagt taaatggata aaacgatcca aatcagctgt tttacttggt
    15121 acgatcactg tcagactttg cgaagcatgt cctttttttc tcaattctac ggtttgagta
    15181 tgaatagcta ggatctcatt tactttttct tgcttctgca gcatatccgc tgctttttga
    15241 cgactatctt ccgtggatga atccaatgta acgattgctt gatagtcaat gatcgttcca
    15301 aattgtttgg ctgaaacaga acttagagaa tctttcaatc ccacgcctgc aaccatcagc
    15361 ccggcacatc cagcaattcc gataatcgcc ataagcattc ttgacttata tcggaagaga
    15421 tttcggtaac tgattttttg attaaagcta aggcgtgacc acagcggagt gatgtattca
    15481 agaaaaatgc gtttccctgg cttcggtgct ttcggttgaa gcaatgcagc aggccgttct
    15541 cttaaatctt tgaataaaac gatcattgca gcgccaaatg cggcaatgaa gaatgcggca
    15601 gctgcttgta aaatcggcgg ccagtcataa aaaataagcg cactcccaat atcataccgc
    15661 tcgttagaca gctcaaaaat gatccttggc aaaagattcg ttcctaatat cgttcctaaa
    15721 acaataccta tccctgaggc tagacttgca tagattgcat atttttttgc gatttcaact
    15781 ttcgtataac ctaacgcctt taatgtcccg atttccctgc ggttttcttc taccatacgt
    15841 gtcattgtcg taaaggtgat caatgcggca ataaagaaaa agaaaacagg aaaaacattg
    15901 gcaatcgcag cgatacgatc agacagatca ccaaattctt gaaaaccgac attttctgaa
    15961 cgttcattgt ataggtatga tggtttagtt aaattttcaa ttttttctgc atttcgattc
    16021 aattcttcct ctgcagaggc cagttcacgt tctttctcaa ctaattgcct ttcttgttca
    16081 gcaagttgag cttcttgttg agataatgtt tgaacagcga ttgttcgttg cgcttcagga
    16141 agctgctgga tagttgctga ctgttgcgct aactgttttt tagcatcttc taaccgtgtt
    16201 cttgcttgcg ttgtttgtgc tttgccatcc gctatttttt gctgtgccgg ttctaattct
    16261 tcgttggccg attttttcag ctcttctaag cgttcttcgg gtcgatcttg taaatacttt
    16321 tctgcctttt cttgattttg ctccatttta tctttgtaag ctttgctgta cgtattcaaa
    16381 ttctttacgt ttgtaaaatc aagatataag acagaataaa tatctgtttt aaaaacggat
    16441 tcaggtaaat agacaaaata atcgacgcta ccatttccga cgttcgcgta tcctctctcc
    16501 aaattgttga tgaacaatgg agaacggaca aagcccgtga tggtatactc tttttctttc
    16561 aattcatctg aatcaatctg ataaatatca ccgattttat acccttcatc ttccgctttt
    16621 tcatccaata caagctggtt tggtttttcc gggagttttc catcagttaa ttgtagcttg
    16681 ttctgcttgt tatttttgtt ataagaaaat atttgaatca cttcgttttt gccagtattg
    16741 gaataataga tctgttttcc tgtttctact tttgcatctg ggatcttttc tgcttgagca
    16801 atatcttttt gtgtcaagcc accagtagaa acgatttgca aatcagaaag tcctgctgta
    16861 ccaacatagt gatccatcgt tttttgcatg acaggtccag cagttttgac acctacaaat
    16921 aaaagtgttc ctagcatgac aataaggatg attgcaatga atcgtccttt agattgccga
    16981 atttcgcgaa aacctgcttt aagatatgtt cttgttttca taaaatcccc ctaccataca
    17041 atctcatcaa tggatgaagg atgatcattt aattcaacag accttacttt agcatcatta
    17101 atgtgaatga ctctatcagc gatgggcgct aaagcagaat tatgtgtaat gatcaaaacg
    17161 gtattgccgt gttgtctgct agcgttttgc aataatttca atacttgctt gcccgtttca
    17221 aaatccagcg cgcctgtcgg ttcatcacat aaaagaagtt tgggattttt agctagtgca
    17281 cgagcaattg aaaccctctg ctgttcgcct cctgataatt gggacggaaa gttattcaat
    17341 cgatgagcta ggcccacttg cctcaatacc tctacaggat caagcgcatc aggagaaact
    17401 tccgttgcca attccacatt ttcttttgct gttagattag gaacgagatt gtagaattgg
    17461 aaaacgaacc ctacatctgt tcgtcggtaa gcagtaagct gtttatctga aaattgcgca
    17521 atatctgtgt catcaataat gatctgacct tcatcaggtg tatccatccc acctaaaata
    17581 ttaagaatcg ttgactttcc tgctccactt ggaccaagta taatcactaa ttcgcctttt
    17641 tcaattgaaa aagaaatatc gtcatttgct gtaatggtcg tttctcccat tttgtatcgt
    17701 ttgtattcat tttttacttc gacatacttc actctgattc ctccattttc acgatatttc
    17761 tcgctatatc tatgcatcga atgcgttttc tcacacttat ttattttacc actgaatttc
    17821 tctagactaa agaaaggaat cctttaattt agttgacaaa taaacgattt atgatttact
    17881 tacataaaaa acattgttat ttgaaagaag gaacatcatg aatgatttaa aggaaaatat
    17941 gatttttgat ttatcaaaaa ctgcttttgt cgcgatcgac atgcaaaaag agatcgtaaa
    18001 taacggtaca ctctctccac acactgcagc atctattctg acaaacaatg atttgttagt
    18061 aagaacattg aaacgtaccg ctgccttgaa agttttagtg aacgtagaca tctccacttt
    18121 cccttatttg agtcaacaaa ctgatatggg tggaatgact gcttctgtac ctccagaatt
    18181 cactgattta cttttgaagg attctttgaa agatacagat aatatgttga ctatcacaaa
    18241 atacaatcca agtgcttttt ttggtacttc tcttgatctt caacttcgcc ggcgtggaat
    18301 cgaaactatc attttaagcg gtgtagcgac aacaaacggt gtatatgcta ctgcattaga
    18361 tgcctttcaa cacgggtacc atatcgtgct tgctgaagat gcttgcagtg atcgtgacaa
    18421 agaatcccat caattgttca taaaaaagat atttccgaaa actgcacgag tacgctcaac
    18481 gaaacaaata atcgaagcta tccaacaaag caaataagta ctggcacacg tgattttcat
    18541 ttacttcttt ttgatttcat gggaaaataa ccacaggatt tagataggag tatgaacatg
    18601 gtgaaaaaaa agatcacaat caaagaagtc gctaaacttt ccggcgtttc aatcacaact
    18661 gtctccttga tattaaatgg aaatacggag aaattcagtc cgaaaactgt tcagaaagtt
    18721 ttagctgcca aagaagaatt aggctatcag cctgactatt ttgctagaca aatgattacc
    18781 aaggaaacga aaacaattgg tgtactcgtt cctgatatca cgaatccatt cttcagtaca
    18841 ctgatgcgag gaattgagga tatcttgtat aagcagaatt tcgtcacgat attatgtaat
    18901 gctgattctg atcatcaaaa agaaatcgaa tatctagccg agctcacacg tcgaggtgtg
    18961 gatggattca ttatcgcgac ttctgctgtc tctacagatg caatcaacga aaatttgaaa
    19021 aaacaaggcc ggcccttcat tgttttggat cagaaaaaat ctgaaggatt tagtgatgcc
    19081 gtacgaacag acgattttcg cggtggttac ctagcgggca tgcacttgct tagtctagga
    19141 catcaaacga tcgcccttgt ctatcctgaa aatccaccgg aaaatgtcca cgctcggatt
    19201 gaggggttta aatctgctct tgatgtttac caaatccctc acgaccaact gatcctctta
    19261 cctacacagt tctcaaaaca aggtggctac cagatcacag cagaacttct tgattctgct
    19321 gcaactggtg tgtttgcttt aaatgatgaa ttagcttttg gattatatcg cggattagag
    19381 gaagctggaa aaagcatccc tgaagactac agcattattg gctatgataa tatagatatg
    19441 tgcgagtata tcaagccgaa actgacaacg atcgcacaac caatcttcga attgggacaa
    19501 acatctgcca agttattgtt agatcggatc caattcccag aaaaagaatg ggaagaaaaa
    19561 agattacctg tacgattaga aaaaagattt tcaactgcac ccttgaaata gctttcaagg
    19621 tgtgctatat ttttatcgaa ctattaaaac gttttactaa aacgttttaa aaaataattt
    19681 tcccatcaaa ataaatgaga tagttggagg tttgtaaaat gaacacggtt acagtaattg
    19741 gaagtattaa tatggacact acccttcgtt taaccaacat gcccaagcca ggcgaaacga
    19801 tgcatgcgca tgagattttt cacgctggcg gaggtaaagg tgccaaccaa gcagtggcag
    19861 ccaaacgttc aggcgcacgg acatctttta ttggaggcgt tggggctgac agcgaaggac
    19921 aacagttatt agatctactg acaaaagaaa acatcgatac atctgggata gctgaaatcc
    19981 aaggcgccac aaccggacaa gcaatgatta tggtagacgc agcaggagaa aatagtatct
    20041 tgatccatgc aggggcaaat aatgctttcc atgaacaaga agtcctaaaa aacaagcaac
    20101 tgattacaaa cagtgacttt attattgctc agtttgaaag tagcttagat gcgacgatcc
    20161 tagctttttc tattgcaaaa gacgcaggaa aaacaacgat cttgaatcct gcaccagctc
    20221 gtgaaacgat tcctactgaa ctacttgaaa agacagatat cattatacca aatgaaacag
    20281 aaacagaaat cattacagga atcagagtga cagatcataa cagtttggtc gcagcagccg
    20341 aaaaactcca tgaattaggt attggaacag taatcattac actaggcagt gccggcgctt
    20401 tctatcatac agaaaaagaa cacggtatcg tccctgcttt taaagtggat gccgttgaca
    20461 caacagccgc aggtgataca tttattggcg cattaagcag cacacttcag ccagacctta
    20521 gtaacttgaa agaagcgatc ctttatggaa atctagcctc ttctgttgct gtgcagtctt
    20581 atggtgcgca accttctatc ccttaccgcg aagctttaga acaatcagca gtctaaaatt
    20641 aacaatgaga ggaagaaaca aacatgaaaa aaggaaaagt aatcaattca gatatttcac
    20701 gagtaatcgc acaaatgggg cactttgata aattaagtat cggggatgca ggcatgcctg
    20761 tcccctttgg tactgaaaaa atagatttgg ctgtctcaaa cggtcttcca agttttttat
    20821 ctgttttaga aaacgtttta gaagaacttg aggtacaatc gattgaattg gcggaagaaa
    20881 taaagacaat gaacccggaa atattggaaa aaatcaaatt attgttacca gatacaccga
    20941 ttagctttat cccacatgaa gaaatgaaaa aagagttgaa taactgtcat gcatttattc
    21001 gtacaggcga aatgacacct tacgccaata ttatattagt gagtggagtg gtcttctaat
    21061 ggaaacgaca atgaatcctg aacagatggt actgtccgag cagattcata tgctgattga
    21121 acagttggga ggaaaagaca atattgattc tgtccttcca tgtgcgactc ggcttcgtat
    21181 caaattgaaa aaacagccag tagccaatgc agaaaaatta aaacagcaaa tcccttccat
    21241 taagtatttc attttactcg atagatatgc agaaataatc atggatcagg gattggctcc
    21301 tgcttacaag gaattgacgc gtcaaatcga tcattcagct ccgaaagaaa agctaaatcc
    21361 gatcgaaaaa ctatttggtt tgatatcgga aatctttacg ccgatcattc cgctgtttgc
    21421 cggttctggt atattaaaag gtctggttgt tttagcgact acgattggct ggcttagtga
    21481 aacaagcgga acttatcata ttttgtctgc tgcgtcgaat gccgtctttt attttctgcc
    21541 aatctttctt gctttttctg ctgccaaaac gtttaaagtg aatggatata ttgcggctgt
    21601 cattgctgca gcactgattg aaccgaatct gacctcttta ctttcagaca gtggcaaagc
    21661 agctactgat tttctgggga ttcctgtagt actgatgcag tatacgtcta ctgtcatgcc
    21721 ggcgatttta ggtattttct tttattcttt tgttgaaaaa ttcctcaaaa aatatattcg
    21781 cgaaaatatg caattagtat ttgtaccttt actttcatta gttatcacgg ttcctttgac
    21841 gttaatggta ttcgggcctg cgggtgttta ccttggcgaa ggtttagctg ccgcaattac
    21901 ttggatgatg gatattaacg gaccgatcag tggcgcaatc atcgctggtg gttggaatat
    21961 tttggtaata tttggtatcc aatgggctgt aaaccctgtc atgatcagta atatctctgc
    22021 ttatgggttc gaccgaatcg ttccgttgac aggagcagcc aattttggaa tggctggtgc
    22081 agctttaggt gtatttctgc gaagtaaacg ttcaaaaacc cgttcgatct ccggttctgc
    22141 ttttgcttcg attttacttg cgggagtgac cgaaccaact gtttacggaa ttgcgatccc
    22201 attgaaaaaa ccatttgttg ctgcttgcat tggtgcagct gctggtggag ctgtaatggg
    22261 atttgcccaa gtgaaagcga tcgcttttgt atttggtagt ctgaccacat tgcctgcatt
    22321 catttccggt acttttttct ggtacttagc aggactggct gttagtctag tcgtagcaat
    22381 gatcactact ctcgttagcg ggtttgatga agacctaatg tcttatgaat agttaatgaa
    22441 aaagactgaa gtatgagatg tttatcacat atttcagtct ttttcttttt aggatcatta
    22501 tttttataaa tttttgtgat actttccaaa ctttggtata atttacatat gcataaccat
    22561 ttttatcccc acttgtttat ccccaaacaa aggtggtcta tgcaataaaa tgaacgtcaa
    22621 agaaaaacga atgaatgctc gttgcttatt tacaaattgt tttatttaga atacacagac
    22681 agatttatga aaagaaaaag cttttcgaca cgattatctg ttccaaaaaa tgtctaatga
    22741 atgctgaata agacagaaaa tcaatgaaag gaaaaggact agtttattat gaaaaataaa
    22801 aaaacgatac gtttgctagc tgctacttgg ctgattgctc cgtctgtttt gtcgaccaat
    22861 accgtatttg ccgtagataa tcaaatagct gatcaaacag tcacaacaga tacgactaca
    22921 tccagctcag ctactagtga aacaacggat tcttttacag aagacccaac tggaacaact
    22981 aactcaacaa caggatcgac cgagccaact gaaattgatc cggctgatcc atcagaaaat
    23041 gaaaagccag ccgagacata ttcccttaca attgctcctt ctttgattgg taagatccaa
    23101 ctgactgctt cgacagcgga ttcccctgaa cagcaacctt tggatattac ggaaagtgga
    23161 acggtagaag gtttattagc ggaaacgaag gtaacttaca ctattacgcc agctgaaggt
    23221 gaacaaattg cttcagtcgc tgttaatggt gccaaagaag taaattatga tgcaaagacc
    23281 tttacagtag gtacgaacaa tttatctatt actgcagaat ttaaagctgg accggatact
    23341 tcaacagaga aaccaactga tcctagtgaa gaagtcccta gtacaccaga tccggaaacg
    23401 ccaaacaaac cagatgtggg taataacaac aataataatg gttcaaataa ttcaaacaat
    23461 aataacaata attcgaacag cgggaattct tcgaatcaaa caaacccatc tggatcgaat
    23521 aacaatggaa atactcagag agaaccaaac catccaaaca atggttcatc gaatggcgga
    23581 acgaacaatg cttcatctcg tccaaataca gcaaatcaaa gcggtataga gaatccatct
    23641 tctgcttctt ctgattttgt tgtaaaatca ccaattgatg ctgtattgcc tacaaataca
    23701 acgaatgtcc aacaagcaat cgttcgagaa gcattcaatc atcttggaaa accatatgta
    23761 tggggagcaa aaggtccaaa tacttttgac tgttctggtt tgacttatta cgtatatatg
    23821 aaagcaaccg gtcattatat cggtggctgg acaggcgaac agcaatatgc aggtacgcag
    23881 attccagtaa gtcaggccca acctggcgac ctcgtcttct ggggaccttc ttccggtgtt
    23941 actcaccatg taggtatcta tattgggaac ggacaattta tccatgcgcc tcaaccagga
    24001 gataaagttc gagtaacttc tatttcagat ttcacgcctg attttgctgt ccgagtaaac
    24061 ttagcaggtt tacctgctgc ttcaggttcg ctgacaggcg gctcatctat tttagatgga
    24121 ttagatggct cgttccattt cagtcaaaac caaacaactg atcagttctt gaaaaagatt
    24181 gcagatgatg cacaggaaat cgggcaaaaa gaaggaatct acgcatcagt catgatggct
    24241 caagctatcc ttgaaagtgg atctggaaac agcttattat caagcgaacc aaatcataac
    24301 ctttttggta tcaaaggatc atataaagga agcagcgtga ccttcaatac acttgaacaa
    24361 gatagttccg gacaaagcta ccaaatccgc gcacaattcc gtaaatatcc ttcttacaag
    24421 gaaagtttgg aagactatgc cgacttgatc aaaaatggac tgactggcaa tcctgatttc
    24481 tacaaaccaa cttggaagtc ggaaacaaaa gactacaaag aagcaaccaa atatttggaa
    24541 ggacgttatg ctacagatcg ccaatattca caaaaattga acgcgatcat cgaagcgtac
    24601 gacctgacaa aatatgatga accgaaaaaa gaagcagcaa caactgaaga agcaaacgct
    24661 gaaatcctca atatgtctaa gcgattcgat gtgccaatca aatggacaac agatggcgtg
    24721 ccaatcacag gattagaatt ttcaacgata aaacgcgtca acaacgcttc ttccatcttc
    24781 cgtgtcgctt caatctggga tttgtggaac aattttacgg caagacagat accagaaaca
    24841 actactcgta cagttaaagc tgcaactgtt cctgtactaa ccttgttaag tttagacaaa
    24901 tcattctggc ttgatctgcc tttaaagaaa tagaataaat taaaaagggg atgtgacact
    24961 gtcacactcc ctttttaatt tattctattt ctttatattt attttgtatt ccgttacaaa
    25021 ctcgcgtact tggttaggtt ccagagtgac atctccaaaa ctttcaagtc gctccgctcc
    25081 tggcagttct tgagtctcaa atgtgatacc gccatgattt actaatttat tcccgcgcat
    25141 ttccggtcca tcttctccaa aatttgctgt aaaaatcacg attcctgaag catctgtttt
    25201 tacttggatc gatacttttt catctgggct tgtcaattcc gcagcattta gtaaaccaga
    25261 ttgttttttc aacaaaaatg gatgatcgat gccgcccact atcattgatt ggcgaaaatc
    25321 actggcaaag acctcaccta attttttagg acgttggaag tcaaatgctg tgccacttac
    25381 tgaaactact tctcctgtag gcgttgtatc tttattgagc gtagcaaata atcggctatt
    25441 caaccaaagt atatgttcgt cgattgactg tgctggatca ccggttaaat tgaaatagac
    25501 atgatttgta ggattgaata ctgttttgcg gtcactgatc ccttggaccg tcactcgcca
    25561 tgtatcatcg ttcgtcagcg tataccgtac ttctacatct aaagttccag gaaatccgtt
    25621 ttccatatct gggctagtct gatagaaaat gaccgatgct tcgttttcac cattgatgac
    25681 tgtataatgc cactgcttgg cttcaaagct aggtgcacca ccatgtaaac aatgaccagt
    25741 gtccgggtct gtttttactt cgaacgtttc atcatctaat ttgaatgtgc cattcgtaat
    25801 ccgtccagcc atacgtccga ttgaagcacc gatataaggg tctttttcta gatattcttt
    25861 tgcttcatca aaacctaaga tcaactctcg tttttcatta tcgattattg tttgaaaatg
    25921 aacaacccgt gccccaagat cactgacaga caaagatgtc ccattttgat tcgtgatcgt
    25981 aatcaagtga tagttttcgc caaaattttg ttcattgatt tccatgttcc ttgcaccctc
    26041 ttttctttca tttgcactga ttccagcata catggaaacc gttttcttaa cagcttattc
    26101 cgtgagtaac gagaaaaaat ttactatatt ctaataaaag accgtgaaaa aaaggttgtg
    26161 acaaaaatat tgtcacaacc tctttttccg aacaaacggt gttcaaaagc ttgcatctgc
    26221 ggtaataagc ccaaccaaac gaaaaatatg aggagctatt tttgcttggc tgttcttatt
    26281 acttgatgca ggacagcttt ttcacaatct ctttttcaat acgattccag tatataacca
    26341 tgtcgtcagt acatagtaga tgaaatacaa tacgaagaat atcgtaaacg gaacccaaat
    26401 atctttgtat ggattttgca tgaatagacc aaacatttgt aatccgaata agacatggac
    26461 tacacctaag attcctggtg ctaagaataa cacgcctact tctctacgga tcgattgttt
    26521 aagcaacccg cgtcgtgttc ctatcttttc aagcatttca taacgagcaa tgtcactctt
    26581 gcttcctgac aagatcttga acatcaaaca gctagcaagc atggtcaaga aagctaaacc
    26641 taggaagaat cccataaatt caaaaccaga gaatagacca ttatacactt catacatttc
    26701 atatttctgg ttgctgtact ctactttttg caaagatgga ttgttcattt tattttgagc
    26761 aacaagtgtt tcgattggtt tcaggtttgc ttggaagtct tttacctgaa ctagctgtaa
    26821 agtggattgt ggcaagttca gttgatcaaa ttctgattga ctgactacct tgatttcttt
    26881 ttcttgctgt tccggcaatt catagcttcg aagttcctct tgtacagtca gttcattcat
    26941 ttttttagca ggaacttttt ctttcgtgat cttcggcggt gttccatctt catgttttat
    27001 catgatcaat ggttgagccg caaattcact tgcattatag taaatcgttc ccgcatcttc
    27061 tttttgagta taagaagcag aaagtgttgg tgaaagttct ttgatttttt gctgatcttc
    27121 tgcccgagga ttattcatca ctagatcata tccagaattg ttttctgtca agtctgcaat
    27181 ttcattttta aaacctaatc ctaccgtcaa agcgcccagt gccaaggcaa acagcatagc
    27241 gaccattgag agcatttgtg tatattcacg aatacgaaag tttaactgtg acaatgtgaa
    27301 attgttcaat tttttcaatg aaatctgttc tgatttcttt agtaatgata aaaagaaaat
    27361 gatcacagaa tggaaaatga aataagttcc taatacgatc gtaactaagg ctacccctat
    27421 accaaagact tgataggtcc ccacgtgttt catcatatag taaccgcagc ctagaaaagc
    27481 aattcccagt actacttcaa ggaaaaacaa gaatttatgc tgcttgattc ttgttggtgt
    27541 ctgatccgca cgaatcaatg ctaaaatcgg ttttttgaca atcgaaaaag cattgacaac
    27601 agcagctaat aaaaacaaaa cagcaaaaaa caaaacagtg atcaacaacc cgtgaatact
    27661 aaaaggtgta aaatgcgaaa tattgagatc taactgatcc actaataatc ggtggacgat
    27721 acttgtcaat cctacaccga cagctgaacc cactaaagta gctgcgatcc ctactaagaa
    27781 tgtttcagaa aaaatcatct gtgcgatttt tctgcctttt gctcccagca tcataaacat
    27841 tgcatagtct ttttgccgca tcgtcatcaa aaaggaattt gcatataaaa tataaacgaa
    27901 tgtgatgatc cctagtaaaa cagaaccgaa tcggaaaatg ataactgtag ccgcaacgat
    27961 cgtattactt ttcaaaaaag cttcgttgct tgccattgat tcaaacatgt aaaaaatagc
    28021 agatgccata actaatcctg agaataacac caagtagtct cttaaacggc tcttgatccc
    28081 agtaagtgat aatttccaaa gcatagtcaa tctcccccta ctgttccaag tttccaagaa
    28141 tagatagtat ttctcgatag aaggaatcac ggctttgatc tccacgtttc acttcttgat
    28201 ggataccgcc atccttgatg aataaaatac gttggcaata acttgcagaa aacggatcat
    28261 gcgtcacaag aagaatcgat acatgatctt ttgtattcaa ttcgtccatc gtatctaaaa
    28321 gatcccgggc acttttggaa tccagcgcac ctgtaggctc atcccctaat agaatcgttg
    28381 gttgtgtgat caatgcacgt gcggcggcga cccgttgttt ttgtccaccg gaaatctcag
    28441 aaggatagct ttccaaaata tgagagatgg ataaacgttc tgcaattttt tggacacgtt
    28501 gcttgatttc ttttggtctg acgccttgta aagaaagagg aacagcgata ttttcttgtg
    28561 ctgtcaagct ttctaataaa ttgaaatctt ggaaaataaa gccaatctca ttcgcacgaa
    28621 aatctgctag ttgatttcct tttaatgtgg tcacatcttt ttgattgatt cggatatgcc
    28681 catctgttgg tttatctaat gtggataaaa tattcaaaag tgtcgactta ccagaaccag
    28741 aagcgcccat gatcccaata aattcgcctt tttctacatc gaagctgatg ccgcttaatg
    28801 cttgcgtctt tttctcattg ttttttccgt atacttttgt tacattctgt acttcgacta
    28861 ttttgttcat ttctttccct accctttaat tagtttttcc agtgctgttt tcggcacgtc
    28921 ttttttttcg acttcttcat acgaagtgac acctttattt ttgttccagg tgatttttaa
    28981 atatgctcct ttacgaagcg gtcgagtcaa ttgtcccttg aagtccagtt ctttttcctt
    29041 accctctttc gtatagccag gcaggatata tctatagttg actgtttctt gtccagagtc
    29101 tgattttgaa actgtttttg taccatctgt cgtaatttgt acatagtatt cttctccacc
    29161 cataactacg taatctgcga ttttcacccc tgcaagtacc acaacgatga aaacaagtag
    29221 accgatcaga agttttttca ctttttcccc ttctctctta ctttatatac tgattataga
    29281 aaaaagcgaa caattctgcc ttcgaatcaa ctatcattct tctttcaatt ttgtcatttt
    29341 ctttaaaaca actaaaaaaa gagttcaaga caataatccg tcctaaactc cttcttccta
    29401 tcttacaaaa agtatgtcac ttacggtctt tcatgtcgcc tgtattgata acaggttgcg
    29461 ttcctttgtc tgtaatgatt gagacaagta aattattgat caattggact ttccgatcat
    29521 cggtgaaatt gatttcttgt ccatcttcga tttgctctaa ggccatttga gtgattgtga
    29581 cagctccttc aacaatggtt tgtcgagcag acaaaatggc ttttgcttgt tgacgttgga
    29641 gcatggcact agcgatttct gttgcatagg ctaaatggtt caagcgtgtt tcgattactt
    29701 cgacacctgc tactgctaaa cgctcttgca gctctttttg caactcgtca gacactgccg
    29761 tcgtgtttcc tcgaagcgtc aaatcattct cattgaatgt atcatacggg tattggctag
    29821 cgatatgccg aatggctgtc tcactttgga tttctacaaa atcttgataa tacgctacat
    29881 cgaataatgc ctttgctgta tcaacgactt taaagactac tactgctgag atttcgatgg
    29941 ggtttccatc caaatcattg acttttaaaa cagaactatt gaaattccga actttcaacg
    30001 aaacagtcat tttttgtgcc aaaggtatcg taaggaaaaa gccattttct cgaatcgtac
    30061 ctaaatattg tccaaaaaac aaaattactt ttgcttgatt aggactgact acagttgccg
    30121 aacttaacaa caataacgtg atcaaccaaa gaaaaatact taaaaaaagt gcccaaactt
    30181 tcgcttgcca catacctaaa tagaagaaaa acaatccaac taaagctaat acgaccaatc
    30241 cgacaatccc tgcataacca ttgatataaa atgctctttt ttctctcatg ctacttcctg
    30301 cgctccttta tccttcaata aatatctaat gttacttttt taattttttt ttcgaatacg
    30361 cgccattaca ccttctagtt ctaactcttt gtcttcagtg atgtaccaac cgcttgcctg
    30421 tgtccctccg gctgcctgcg gtaaataatc caattttgtt cgaacccagt gcttttcttc
    30481 tgtaaaatag tcttctacca agacctctag atccatccct gctgaaagag gcaaatcttc
    30541 ccgaacagat ttaaagataa atcgttcctt cttcccatca tacttcaata ttcctgtatc
    30601 gataatcggt aaagaataat aatccagctc tttcttcacc gtcaataaat gtttgaaaac
    30661 ttcatggtac acttgctcta attggcgacc ttctgaattg ttcgggtaat taaccatcac
    30721 tttttctttg tctggatcat agaaggtttg gcttagttga gtgatataag tatgtaatct
    30781 ttccatttct gtttttaact tttctctttg accttcttct accttcatat actctttcac
    30841 cctgttttct ttttagatat tttctttctt attttactat agttatgtgc gttaagcgat
    30901 gaaaaacagc aaagattgga aggatctttg ctgttttttc ttaaaatatc cagttgagta
    30961 caggatcaat ctgtttcatt gtcctcttga tccgtcgtaa tattgatcga tgtgtcttta
    31021 gcagtgatct ttagcttttt ctgtccttgt tctaacaaag tatttgttat tggctggtag
    31081 tctttttcaa tataagattc accagacgtt tggatagtat agccactgtt ttccatctca
    31141 tgcaaagata aattgccacc gtttaggatg acttttcctt ctttgctgat caaactgtct
    31201 tctagatgga agttgctgtt ttcacctgtg acgatcaagt tatccacatt ggcctcatct
    31261 aatcgaagtg agcctaattc atctttcgca gttattgttc ctcctgtaaa ttcattgaac
    31321 gatacccagc catcatgtgt cttagtattc acttttttga ttgatacttc gttgatatca
    31381 attgcactat tattcccttc gattgtcaat tcagataatt catgatctgc agggacaaca
    31441 atagtgattt gtgcacttgt gtcttctaaa ctaatgattg cacctgttga ttttttgact
    31501 gcattcacag tcaacacgcc atttttctcc tttatttctg gcttttctgt cttatcataa
    31561 gtcgttttta tctcaaaatc caatccgcgt atgaccgaaa ctgtttgatt ttctgcatgg
    31621 atgacgattt tgtttgcatc cttgattttt tttgtctcag taaccatatc gatcaaccga
    31681 ggaccattat cccaagtcag actcttttga gcgcctaatg tataagttgc acctgctagg
    31741 aggccaccag taagcatcag accaataccg gtaatcaaaa aatgtttcaa tctcaattta
    31801 tgtgtcctcc ttcaaacgcc cgctgaatgc agatctcttt ttgtaataaa acgtcgtccc
    31861 atccatttaa caaagttcat catgacataa aaaagccctt ttgttccttt cagcaacagt
    31921 ggcgaaataa atactgcgct tcctgtggcg aacagaccga caccgataaa gaataatcca
    31981 ctcagaatcg attgtccaaa cacactgatt cctgagatga tcatgaatag gccaacacct
    32041 aagatactga tacataaaac atagatacta aaaatgagcg ctagtaaagc aaccaatagt
    32101 cccccaataa ccaatataaa ccccagtgct agtggaatga ggacaggaga agcaaataaa
    32161 ccgataatga ttaaccaaat caggcgaatt tgtctttttg gatttttcac taactcgttt
    32221 tctgcttgtt cttcttctga aaagtaactg atttttaatt ttaaggcaaa ctgctttggt
    32281 gtaccatatt catcaatgat ctgatctaat gtttttccag aatctaaaaa atattcctca
    32341 taatactgga tcaaatcaaa acgatcgtct tcaggaattt cttgaagatg atccacaact
    32401 tcattcaaat attcattcat ttgtgctctc ctccaataac cggtctaaag aatctcgaaa
    32461 aacaatccat tcatcaataa tttcttctaa atgacgactt cccattggtg tcaacgagta
    32521 atatcgacgg tttctgcctt caaaaggttc atcataggtc tcaagatatc cttctttttt
    32581 caatcttctc aaaaccggat atagtgtaga ttcactgaca gagagacttt cttgtacgat
    32641 ttttgttaag gcatacccat acatatcttc cttgcttaat gctgctagca cagtaccatc
    32701 aagaagaaca gttggaatct ggatgtgcat tacgtcacct tctattctca agtgtaatat
    32761 tttatttcaa tctatattat acaacatata atatagtttc aaaatcagtt tgtcaaatac
    32821 aaaaaaactg actaaaagat ttgatccttt ttagtcagca aaaaatggct tctctttgat
    32881 ttttattata acagtggcat aatccaattt gtccacatcc agctcagtgg catgataacg
    32941 atcattgcag gtatcatgtc agctactggg aacattttca cgttgatcat tctaaaaccg
    33001 gtagcgacca tcaagaatcc gccgcaagct ttaaaatcta agatcatgtc tggtgtagtc
    33061 aatggataaa taaattttgc caataagaac aaaatataaa aaataatgaa ttggggaatt
    33121 gctatcacag aaacgacata ccctaaatta cacgcaaaaa tagctgctgt gaaaaaatca
    33181 aggacagatt tagaaatcaa aatcgtactg tctcctgtca tcccagaatc taaacttcca
    33241 tagatccccg taccacttgc acaaaataga acaatgaccg tcactaatgt gctgataaat
    33301 tcatcgtggg tcataccaag cttttcacta gggaagattt tagaaatagg tacttgcatc
    33361 agcgcagccc ccttatttat cccattacct aaatgaacaa tcaaaccaat cgctgttcct
    33421 atcactaaag caaaaataac agccggcata tttttcatcg gcgcaatgga atagattccc
    33481 atccccatcg aacacacacc aaaaaccatc gtcagctgtg ctttaaaatg ttcacttaat
    33541 ttatttccta ctaatccgcc aattagtccg ccaaacaaaa cggaacctac attgatcaaa
    33601 actccagttg gcatcaaaaa actcctctct ttttctacga agcgaaaaaa atcagaaaaa
    33661 tttctgtaat tattgctttc gctgcaagaa agattgtaac agacagagaa gattacacca
    33721 attcattttt tgtgttaaag tattctttat tggaataagt aggaggaata tgatggatat
    33781 ccataaattc gaagtttttc ttgatttagc cgaaacaatg aatttcacca aaactgctga
    33841 tcgacaattc accacccaag gaaatatttc taaacagatt ctttcgttag aaacagaatt
    33901 agacgtcaaa ttattcgaac gcgcccatag aaaaatcgaa ttgaccgaag ctggaagctt
    33961 attgctccca tatgtaaaaa atgtcgtaga acaataccat gctatgcaag aagtgcttga
    34021 aacctatacg aaagacaaca atctttcatt aaatatctta acgataccga caatgatcaa
    34081 ttataaaggt ttttccaaaa tcaccgagtt tttgaaacgc catcctgaat ttacagtcca
    34141 gttgaaagaa gtggaaagtg tcgaattaac ttacaatgac gccaatgaac atagtgacac
    34201 gatccacttt gctagaagtt tccaggcccc accaagttcg attgagtggt tgcctactga
    34261 aacagacgat tttgtagcag ttctacctaa aaatcatcct ttagctagta aaaagaaatt
    34321 ggatctaagc gaactgaaaa aggagaactt tttgctcctt ggccccacta cgaacttgta
    34381 tcaacctgta ctagatctgt gtcgcgaagc tggatttgaa ccaaaaatct cttataaagg
    34441 agcaagaatc gacttgatca tcaatatgat tgcaaatgac atggggattg ctattgttat
    34501 ggaaaaaaca gtcaagaatc tattaaaaga aaatactgtg attctcccta tttctccaac
    34561 aacagagagt tatcttgcat ttactcgtaa agtcggtgag cattctttgg cttcagatac
    34621 attttgggag tatttaaatc aagaagaaaa ctaagcccta cattttctgt ccatctatcc
    34681 acccaaaaaa agagagcgac gcatgacttt cgctctcttt cttgcatcat gtccttgttt
    34741 caatgacacc tctaacattt ctcgtagttc aataagaaag cagcgatttt tatgccaaaa
    34801 gaaccgtaag aacatttccc taatcacaaa aaaacgacta aaaaccccta aaaaccttca
    34861 atagaatttt taggggtttt attctttttc ctattgttta cttaaccgta tcattgctgt
    34921 cgttcctact ccttcgatac tgctataatc aatacgtcca tcatgcagtt ctagaatttt
    34981 tttaaccagt gaaagtccta gcccattccc agaaatttga cgacttgtat ctccttgata
    35041 aaatttttca aacattcgat ctctggtctc ttcattcatt cctacacctg aatcggtcac
    35101 ttccagtaca atctctgtgg ctgtctcaaa tagtttgata tgaatctgtc cattgatttg
    35161 attgtacttt atggcgttat ccatgatatt cagccacact tggtacaaaa actcttcatt
    35221 gcctgtgtaa tttactgcag caagttcgat atccaattct agctgttctt tctcccattt
    35281 aggctgaaaa aaaacgatta cttctctgat ttgttcatct aatcgatact ctttcttttc
    35341 cagttggata cgctggtttt ccagtttgtt caattttaag atgttctctg ttaattgtga
    35401 aagctgcgtg atactttcga taatccgata taaaaatatt tggcgttctt cgtcagagag
    35461 atttggggct tgtaacaatt gaacatatcc ttggattgtt gctagtggtg ttttgaattc
    35521 atgggataca ctggacacaa agtcattcct cagtgtctcg atactattga gttcttggac
    35581 cataacgttg aaatctttat acagttgctg tacttctgct actttctgct gttcatccat
    35641 tcgaatcgaa aaatctcccg ttgctaccag actcatattt gtccctagcg cactgattgg
    35701 atgcaggata cgccgcccaa caaaaatcga tattcctgta cccacgacca tactgaaccc
    35761 cgcaaagacg aataacgggt ataacgttgg ttttccagcc gattcagcag gcgagataga
    35821 atgtcgagac aataagaaaa taaaaccgag aaaacacagc agtgtcacta agatcgaaat
    35881 gaagacaata ctagcaaaat agatccataa ttgtgaaaat agcttttttt tcatctttaa
    35941 ataaccgcct tatagcctag tccccgtact gtgatgattt gaaaatcttc cgtcttttca
    36001 aaacgggtcc ttaaacgttt gatatgtaca tcgatcgttc gttcttctgt attcgtatct
    36061 aatccccaaa tatcatccat caactgttgg cgagtgaaga tcttgttggg ataagacagt
    36121 aatttataca gaagcagaaa ttctttttga ggcagaatca acgaatgctg atcttgtaac
    36181 actgtataag catctagctc taattttgtt tgtccgatta tcaagctact gctgtaattg
    36241 atctttgaac gtcgaagcaa tgcttccaca cgtaggatca tctcattgac atcgatgggt
    36301 tttgtcatat aatcatctgc tcctaatttg aacccgcgtt tcttatcctc aaaatcggcc
    36361 tttgccgtaa tcattagaac aggtgtgtca aatctggtgc tacgtaatga gtctagcaag
    36421 tcatagccat ccattttcgg catcatgata tctgtaatga ttaattctat acgtgtcgct
    36481 tctagttttt tcagcgcatc ctctccattc aatgcggtga tgactgaaaa gcccgctcgt
    36541 tccaacacag aacggtaaag caactgtaat tgcggatcat cttctactac taaaatacga
    36601 ttcattgatc tccctctgtt ttttgaaaaa tttctagatt ttttcttgta gttcatcttc
    36661 agttaacatt tatttcgtat actacgatca ttgttctgat atacttatta gtataccgaa
    36721 tatttcaata cgggagaagg gtgattatgc ttcaattaaa agatataaaa aaatattata
    36781 aagtcggcga aaccaccacc aaagcattgg atggtgtgtc tgtcgctttc agacaaaaag
    36841 aattcgtagc gattttaggt cctagtggtt ctggtaaaac gactatgctg aatgtgattg
    36901 gtggactaga taattatgat tccggtgata tggtaatcaa cggaaagtct actaaagact
    36961 ttaaggacag tgactgggat gcctaccgaa acaactctat cggtttcatt tttcaaagtt
    37021 acaacttgat tggtcatcta ggcattattg aaaacgttga acttgggatg acattaagtg
    37081 gtgtttctaa agacgaaaaa cgaaaacggg cagaagatgc attgcaccga gtcggcttga
    37141 caaatcatat gcataaaaag ccaaatcagt tatctggtgg acaaatgcag cgagtggcca
    37201 tcgctcgagc actagccaat gacccagata tccttttgtg cgatgaacca accggagcgt
    37261 tagatacaga aacaagtatc cagatcatgg aattgatcca agagttatcg aaagaaaaat
    37321 tagtgatcat ggtcacacat aatcctgaac tggcccatca atacgcagat cgaatcattg
    37381 aattttctga tggtaagatc ttaacagatt cccatcctca catcgaacga ccaaaagatg
    37441 atcaatttaa cttacgcaga acaaagatga gtttttggac agcgttaaag ctttccttca
    37501 acaatatccg aacaaaaaaa ggacgtactt tcttgacctc ctttgcttcc agtattggga
    37561 tcatcggaat cgcgatcgtt ctgtcccttt cttctggatt ccaaaaacaa atcgataaca
    37621 cgcaagctga aacgatggcg aaatttccga ttactatttc taaggtaacg actaaccaga
    37681 ctcgtgacga tgccggattg ggtgcaagca aagcggatta tccagacagc aaaacgatta
    37741 ctgcaaaagt cagcgaagaa gataaagcac agcacacgaa taagattgat caaacatacg
    37801 tcgattacgt aacagacatc gatcctaact taagtaataa tatcggattt acccgtacta
    37861 caggaatcaa tcttttacgt gatgtaaatg gcaaagtaca gcctgtcagc ttctcgaatc
    37921 aaaatcctga tacggaatct ctttctttat ctagtacgat gtcagcaatg acaggtgtgg
    37981 gagtgtcaag tttccctaca cagcttgaca ctagcaaaga aaacttttta aaagacaatt
    38041 attctttact tgccggaagt tatccagcct cagcaacaga tgtcgtgctg atcgtagacg
    38101 gcaataataa tactaatatc aacgcattga agaatcttgg atttgatgtg aaagaagacg
    38161 aaaaacttga ttttgatgat atcgttggta caacattcaa attagtaaac aacaatactt
    38221 actatacaaa acttccaact gggaatttta tccctaatac agattatgat gcgatgtatc
    38281 agaatgcttc tgatgaattg aagatctcgg ggatcctacg cgtgaaatct tcttctacaa
    38341 tgaatctctt atctccagga atcgcttata gcgatcaatt aacgactcaa atcgtcaacg
    38401 aaaacaaaga atcagaaatc gtcaaagctc aaagagacag tggtgtaaat gtcttaacta
    38461 ctgaaaaagt tgacgaaaat gccaaacaaa cattgcttag ttatttaggt ggagacagct
    38521 tgccttcaag tatcatgatt tatccgaaca attttgaaga caaagaaaag atcttggatt
    38581 atctagatga ttacaataaa ggcaaatcag atgaagataa aatcatttat actgatttag
    38641 ctggaacgat gactgaactg actggcggat tgatggatgc tatcacttat gtccttattg
    38701 cttttgcagg gatctcttta gtaacaagta tgatcatgat cagtatcatt acttatacat
    38761 ctgtgattga acggacaaaa gaaatcggtg tactaaaagc tttaggtgca cgtaaaaaag
    38821 acatcactcg cgtatttgac gcagaaacgt gtattttagg tatttcttct gggatattag
    38881 gtgtgtttat tgcatggcta gctacgttcc ctatcaatag catcttatac aacatgacgg
    38941 atctaaaaaa tgttgctcaa ctaaatcctg tccatgcgat tattctagtg atcgtctcta
    39001 ctatattgac gatgcttggc ggtcatcttc ctgcacgaat ggcagcgaaa aaagacgccg
    39061 ctatcgcttt gcgtgcagaa taaggttgtg acaaccacat ttagatttga gtcataagag
    39121 cgctgaaata gtagaaaaca gcaagaaacg aggaagtaat atgttccttg tttcgcgctg
    39181 ttttttttaa ttatttttta taattctggt atacttttct ttcctttgta ccacaactct
    39241 tctggcgtat acctgtcttt tccatttatg aatactgttc catctgcttc aatcgttact
    39301 tcagtcccaa tccacatatt acgagaaagt gcgagaagat tttttctttt tcctcttcct
    39361 ttttttccaa attgcaaggc atcggttatc tcttctgtcc aatgagattt cacgtatttt
    39421 acttctccgt tttcaaatgt aactttcaaa atattcgcta tattttccac ttttactgct
    39481 ttaggaaatg actcctccat ttttttcact ttccttcctc caacactaag tatgcgcttt
    39541 cttttagtga taagtatatc atagtttatt ctattatcaa aaaaacgcat ccattccatt
    39601 gtagaatact ttaccgaaca agttgaattg ttttcctctt tttcaccttt tactatagat
    39661 aggtaaagac aactaaggag gaatctctca tgacagatca accgtatgat ttaagaaaag
    39721 tcatcgaaga actaaaaaca cttccaggtc aatatcacga aacagatatt gaaatcgatc
    39781 ctgaagcaga attatctggt gtttaccgtt atatcggtgc tggcggtaca gtaaaacgtc
    39841 cgactcaaga aggaccaact atgactttca acaatatcaa aggtttcccc ggcacacgtg
    39901 tgaacatcgg aacaatggct agtcgtaaac gtgtaggtga aattttgcac catgattatc
    39961 gaacacttgg tcgtttcttg aaagacgctg tagaaaatcc aatcaaacct gtgaaagtag
    40021 acaaagcaga cgctccagca caagaagtcg ttcatctagc taccgatgca gacttcgata
    40081 ttcgcaacat tttgcctgca cctacaaata ctcctgaaga tgctggtcca tacatcacaa
    40141 tgggtgtagt cttaggatca gatccagaaa aaacaatgag cgatgtaacg atccatcgta
    40201 tggttctaga agataaagat acgatcggta tgtacatcat gcctggcgga cgccacattg
    40261 gtcacttcca aaaacaattc gaagccttga ataaaccaat gcctatcact atcaatattg
    40321 gtttagatcc agcaattgcc attggcacga ctttcgaacc acctaccact ccacttggct
    40381 acaatgaact atggtgtgca ggagctttgc gtaacgaacc tattcaaatc gttgatggta
    40441 tagcagtcga tgaagtgggt attgctcgtg ctgaattcat cattgaagca gaaatcatgc
    40501 caaatgaaac aatgcaagaa gatatcaaca cgaactcagg aaaagcaatg ccagaatttc
    40561 ctggctatac tggggatgct aatccagctg tacaagtagt gaaagtcaaa gcagttactc
    40621 accgaaaaga caatccaatc atgcaaacaa cgattggtcc cagtgaagaa cacgtatcta
    40681 tggccggtat tcctacagaa gcaagtattc ttgccttggt cgataaagct attccaggaa
    40741 aagtagtaaa tgtctacaat cctcctgcag gtggtggtaa attgatgacc atcatgcaaa
    40801 tccgtaaaga aaacgaagcg gatgaaggta ttcagcgtca agcagctatt cttgctttat
    40861 cagccttcaa agaattgaag acagtcattt tagtcgatga agatgttgat attttcgaca
    40921 tgaacgatgt agtttggact ttcaacactc gtttccaagg agataaagat atcatcgtga
    40981 tcccaggaat gaggaaccat ccattggacc catcagaatt accagaatat aatcctggct
    41041 ctattcgttt ccgtgggata agtgcaaaaa ctatctttga cggtacagtt cctttcgata
    41101 tgaaagaaca ttttgtacgt gcaaaattcc aagaagtaga agattgggaa aaatatttga
    41161 aataatgaat gatctgcaac tgttttgaca tcataaaaag gagtgcggaa aatggaacaa
    41221 ttggcacaac ggaagaaaaa aatcgtgatt ggtgtaagtg gcgcttcagg caccatttat
    41281 gccatcaacc tagtgaagaa actaaaagaa tatccgatga tagaaacaca cggaatcatc
    41341 agtccatggg caaaacagaa cttaaagctg gaatccgact tatcactaac tgaattcgaa
    41401 agcctgtttg attaccatta ttcaaacaaa gatttaggtg ctgctgtcgc cagcggatca
    41461 tttttgaccg atggaatgat catcgtaccg gcaagtatga aaacagtcgc aggtatctct
    41521 gttggtttag gcgacaacct gattgcacgt gctgctgatg taacaatgaa ggagcaacga
    41581 aagttagtca ttgttcctcg tgaaacgcca ttaaatgcca ttcacttgga aaacttgact
    41641 aaattagcac gactaggtgt gcaaatcata ccaccaattc ctgcttttta taatcagcct
    41701 caaacgatcc aagatcttgt taatcaccat acaatgaaac ttttggatca atttgaaatt
    41761 gtaaatgagc attcaaaacg ttggcagggt gattaatatg gaaaaagaaa cgatagttga
    41821 agtaaccaaa ctaggtttca cgatccaagc tgctgttaaa gtaatcggta aagacttatt
    41881 gatcacttta acaggcggag atacaccaca tatcggaaca gtcactacat tttctcgtga
    41941 tacagagatg caaactgtac gtttccctag ccacgatggt cggttccata aagacgatgt
    42001 cttagccgaa aaaatagctg tgatcatcaa aactgccgtt ccgggaagtt gtacgatcac
    42061 ttccggtatc catgttgacc atatctccaa tgcacaaatc aacgcctctt ttccgatgtc
    42121 agaagagtta ggttacaaga ttttagattg gttggaaaaa atagatttcc atatcaaaga
    42181 accagtttat tataaagatg gtgaaaaacc attatgaaag aagaggttgt gaaatcagcg
    42241 ttatgacctg agtatgagag aaaaaaatgg aaaaatagct tctcatattt tgccgttttt
    42301 gagctaatac cgagggacag ctgattgaac gccgtttatc aagaggttgt gataaagccg
    42361 cttcgtttcg agtagtaaga aaaattttgt aaacatgggt ttccacattt ttgcataaat
    42421 ttgacttact accgagaaac tggcttttga acaccgttta tcaagaggtt gtgacacgtc
    42481 ttttgtcaca acctctttta tttttgcttc actgtaactg ctacaatatc accgatttct
    42541 tttccgatgt ttttacgaat atcttttcgt agtccaagga tgtagcagac tgaaccatct
    42601 ggatttttca cgcccatatt cacgatactg ccttcatatg gcacattgtc aaaagttgct
    42661 tggactttaa ctcttccagc accgaattct tttcttatat catacggaaa aataatatat
    42721 gccccgccct tgccgacttc ggaagcatag atttttgatt caaaagaata aatttccctc
    42781 attttatttc tcctattttt tacatctagt atagcatagc aaaaaaaaga aggaactgtg
    42841 acaaaagtct tgtcgcagtt ccttttttcg aataaacggt gttctcctgc caactcctcg
    42901 gtcacaagtc acaatagtca acaaacatga gaagctgttt tttgaaattg ctccttgtga
    42961 ctcagagtta aacggcaggc tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc
    43021 tacggcacta agcccaatca gatgaaaaat atggagtgct atttttatct gattgttctt
    43081 actgcttgat gcagagcagc tttttcacaa cctcctacat ggtgttcaaa atattattcg
    43141 ccttttgctt ctttcactaa ttttgcgaat gcttgttgtt ggaattcagc agctaaggct
    43201 ttcgtacgtt gttcttgctc tgctttgctc atttttggat tttcagtcat tgcttttacc
    43261 ataccgtctt gaacataagc ttttgcttca ctttccatcg tactttgctg tttcttcact
    43321 aattcagcta acttggctgc ttggtccgta cttaattcta cccatgtctc tgggattttg
    43381 aatgtattca ctcggctgtc atattcgtga ttgttatcag caatattgaa ccagaattta
    43441 tacatgtcgt ttttgtattc ccaataagta gttttcgtca ctttttcagc atcgccgtct
    43501 gtcttttcta ctttatttgt ttcgttatcc gtaccagttg cttttggttt ttcttgtttt
    43561 tcatttgttc ggtaaagata cactttttct tttccattcc ctaaagcttt atacaaaagc
    43621 atatctgctc cttgggaatc agcactggaa acaatttttt ctgtttttgt ttccgttact
    43681 ttttccattc caaagtgatt ggacaaatta gcgacgatca aacttagtga agcgacgaag
    43741 attagtccga aaatcaaaga taaaaaggtt tgccatgttt ttttagcgaa cacgttcgtt
    43801 aaagcaaaag caagcaagct gacaatcaaa actccaataa tcatgcatta tcccccgctt
    43861 tcattagaac aggttctttt ttcttcaata aaaaggcaat aataaagcca agaatcccaa
    43921 agatgatcgc tacgataaac gcggcatggt aacctgacag tgtggcattg attgcctgat
    43981 ctttataagc taatggcgta ttatccaata cagatttgct tgggatattt gcttttgtca
    44041 cattcgtcaa cacactgatc aaaatagctg taccaatcga gcttgcgatt tgacggaatg
    44101 tattatttac cgctgtccca tgactgatca agttatttgg caatgcattc ataccagaag
    44161 tcgttaccgg catcatgacc atggagatcc caaacatacg gatcgcataa aggatcacga
    44221 tatataaaac tggagtcgtt tttgtcaaga aagcaaatgg tgcagttgcc gctgtcaaaa
    44281 tgaacatccc tgtgatggct aaacgtttcg cgccgtattt atcgaaaatc gcacccgtca
    44341 caggcatcat agcccccatg atcaatgcac ccggcaacaa catcaagcca gaatggaatg
    44401 ctgattctcc acgaatattt tgaatataca atggtacgac catttctgcc cctaccatcg
    44461 ccatatttgt tacaccagct aaaacagctg caatcgtgaa tgtagatgat ttgaatacac
    44521 gaagttccaa gaatggatgt tccaaatgta attgacgcca tgagaacaag gcgatgaaaa
    44581 tcacaccaac aactaagaaa ccaattactt tcaagcttcc ccagccgtca tctcctacac
    44641 tagaaaatcc gtaaagtaaa ctaccgaatc caatcgttga taaaatagcg gataaaatat
    44701 caatgctagg atttgacaat ggaataacac ttttcattag gaagaatgct aaaaccaaga
    44761 caagaacaac gattggcagt accataccaa acaagtcacg ccaatggtag ttatcaatga
    44821 tataaccaga taatgtaggg cctaaagcag gagcaagtcc aataacgatc cctgacatcc
    44881 ccattgctga tcctcttttt tctggcggga aaatagatag catgatattt tgcaataatg
    44941 gcatcgatac tcccacaccg caagcttgga tcaaacgacc gattagtaat gtactaaaat
    45001 taggtgctgt aaagcaagta atcgttccga tcaaaaagat cgtcatggca gaaagatata
    45061 aatttcttga actgaattta ttgatcagcc atgctgttat tgggatcatg atcccgttga
    45121 ctagcaagaa accagtcgtt aaccattgca catcagacgc tgaaatatcg aaagctttca
    45181 tcaatgtagg aaaagctgtc gtcaataagg tctgattcaa aacggtacaa aatgtaccaa
    45241 ttaataagac aacaactaaa agtgtccgat tatatggttt tccgtaaata tctacgggtt
    45301 ggttgttact catatttctc cttctttctg aattttttct gaaactacta gaatacttta
    45361 gtcctaaatt cgctatttgt caacctactt gacacataag ttaatacgga tataattaag
    45421 ggaagcaaga ggacaaaagg agtgacaaaa atggttgggg cttcccttaa acgtttgttg
    45481 gaaaatgatc aattggttgt tggcatcagt gaattaagtg atatcgtagg tgtgtctcca
    45541 cgtcaattaa ggtattggga acaaaaggga ttcattcgtt cgatcgaagc tgatgcgaat
    45601 tgtcctagaa aatatcgttt acctacagtc ataaaagtag aactgatcaa aaaatattta
    45661 gatgacggat ttactttgtc aaaagctgca gaaaaggcag aaatccgcca aaagaaaatg
    45721 caccatgtcc gtaaagtttt ttcaaaagca atcaaagata tcgaattgat cgacgaacgc
    45781 tatacgattt tacaaatcgg taacttcaaa gaagagaatg ctaagatgta catcattcat
    45841 gacaacgaga cagacgaatt aacttacaag ctccttcctg tcgatgaaaa agctgactac
    45901 gaacagttgt taaaagaact ctaaacgcca ctaatctcct aagtaccaag ctttttaata
    45961 agtagatgct acataaatac ttgaaaaaag gcctgtttga acataagaat atccgaacgg
    46021 tgatctgcgt ttctttcata tagaaaggaa ttctccatca ctgttcggat atttttttat
    46081 ttttttcggt tactttcttc taatacatct gttagccaat ttgcatattc tgtttcccga
    46141 ttgatcgcat gcgctaaaat caggtagtgt ccgtaatttt catgtttttc tttttcagta
    46201 ggaaagatcg ttttttttcg ctctaataga tgaagtcgtt tttcttcatg aaggtcacgc
    46261 tgctgctgaa taatttctac taatgccgga tcattcacgt ctttgacgaa atacaatttc
    46321 agaacaaatt cgtctcgatt aaccggtagt tcattggtgg gtgtatgaat ccactctgcc
    46381 aattgttcct tcccttcttc tgttaattca taaactttct tttccaattt ttcacctgtg
    46441 atctctactt gatgctgaat gattccttgc tcttccattt ttgcaagttc aggataaatc
    46501 tgactatgtt ttgcttgcca aaattctcct atttcatttt tgaacgattg attcaattca
    46561 tatcctgtca tcttcttctt acttaataac cctaacaaaa tatatcgaag tgtatttttt
    46621 cttgccatca gttttccctc cttgacaaat ctttaaatcc attctatcat aaatgagtaa
    46681 tcgaaaacat gtagtttatt acatgtttta atttataagg agatgcttaa acatgaaaga
    46741 atttaaagaa ttggaagatt ttattggaac tcactttatc tatacgtatg acaatggttg
    46801 ggaatatgaa tggtatgcca aaaatgacca tactgttgat taccgtatcc atggcggaat
    46861 ggtcaaaggc cgttgggtaa aagaccaaga agctgatatc gttaaattga cggacggcgt
    46921 attcaaaatc acttggacag agccaacagg gacagacgtg gcattggact ttatgccaaa
    46981 tgaaaagaaa cttcacggaa tgatcttttt ccctaaatgg gttgaagaac atccagaaat
    47041 cactgttact ttccaaaacg aacatatcgc agaaatggaa gcagctcgcg aaaaatatga
    47101 aacttatcca aaattacttg ttcctgaatt tgctacgatc acttatatgg gtgatgcagg
    47161 tatagacaat gaagatgtta ttagcgaaga accatacgat ggaatgccag atgatatccg
    47221 tagcggaaaa tacttcgatg aaaattataa acgagtaaaa taaggttgtg aaagagaggt
    47281 tgtgaaatca gcgttctgac ctgagtatta gagaaagaaa cggaaaaata gttcctcatg
    47341 ttttgccgtt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tatcaagagg
    47401 ttgtgaaatc agcgttatgc tttgagtaat aagaacgcta gttagaaaat agcttttcat
    47461 attttctagg ctagtgggct tattaccgag aagcagccgt ttgaacaccg tttatcaaga
    47521 ggttgtgaaa tcagcgtttt gacctgagta ttagagaaac aaatggaaaa atagttcctc
    47581 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttatcaaa
    47641 gattgtaaca actgcatatt cacacagtta aattagtgac ttcaaaatga aataatgctc
    47701 ccagcaaaaa attaggctgg gagcattatt tcgttattct gttcatcaaa aatcagcgtt
    47761 ttgattccat ttcatcacaa gttcctcctc atttactgct tcatcttttt gctgtgtttg
    47821 aattgtaaaa ccttgtgaaa gatagaaatg aatagctgat tgattttttt gatagactgt
    47881 caaagtcagt gttttattgg tttcttttat ttttttcatt agttgcgtac cgattctttg
    47941 ttgccggtat tcccttttca caaagattcc tgcgataaac tgatcaatca ccccagcaaa
    48001 accaataatt tcttgatcta tttctgctaa gtaaatcact gcatctggta aagctttcct
    48061 aacagattct acttgattct cccaataagt tgatggaatg aattcatgag aatccaagtt
    48121 agcatgcagc caaatcgata gaacagattc cagttctttg gcagacaatt ttgttgttgt
    48181 gcggatcatt ttttctctcc ttacttattc attgatttta aaataaaaac catttttttg
    48241 aagctctttt acaagttttc gcccctcaaa aaagtatatc ttctcttgtt ttacttttct
    48301 aataccaaaa gaaacagcaa ctttaggatc atcagctgaa acgattcgat aagttccacc
    48361 aggcaattga tccagcttga ctttcccatg atggtcgctt tgtactgaaa acggcacacc
    48421 atttctagta agtgtttttt tcttatgttt caaaatgaat gaactaccat ttaccggttt
    48481 ccctgacgaa tcactaataa taaatgaaaa atcaagcact tttttactta atctatactg
    48541 atagattagt cgtccgataa acactaacag tatgactaca gctattatcg ttcctgcaag
    48601 aataaaccat tgccgcatgg ttctttcctg atttcctttt ccttcttctt tttttaccga
    48661 ttcgttataa ggaatccgat gtcctgtcac tagcaaacga tgcgtgttga tcatatacgg
    48721 cgtacatgta agcaaggtaa ctaagtcttc tccttctgaa atcgttagaa cagacgtgtc
    48781 atctggcagt accacttgta tatttttaac ttgataagcc aatttctccc ccaaaacagt
    48841 caaaataaac gtgtctccgt tttctaaacg atctaattcg gtaaataacc gtcgattagg
    48901 taaaccgctg tgagcagaga tcactgaatg cgtcgaattt ccaccaagcg ggaaagaggt
    48961 tccttgcaag acgcctgctc cattttcaag gatcctacca ttgagccgat cataaagtga
    49021 aatatttaat tggatcttag gtatattgat acttccgatc agccattcct ctaattggct
    49081 ggatgttgta ctttcatttc tgttcttttc attgaaagga tcagtaccag gattgaatcc
    49141 ttggtcagtt aatcgttggt ttttctcctc cattttcttt cgctgcactt caatttcagc
    49201 tgcggtccgt ttttgtactt gttcgatacg tttttgatca ataaaactat ttaaagaatc
    49261 cacataaaat ggatataaag cgatcagtaa ccctacgacg atacatatta ataaaaacaa
    49321 acgaaacaaa agtcgacgag actgtttttg cttactctgc ttcacatgtg tcctcccttg
    49381 caagtttttt tgttctttct tttattatat gggttcagga aaaaaataga aaatgaaagc
    49441 cgatgaggtt gtgaaaaagc tgtcctgcat caagtaataa gtacagccca aaaaaatagc
    49501 tactcatatt ttttgttggg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    49561 taaggaagag gttgtgaaaa ctgcgttatg ctttgagtaa taagagcgct agtcagaaaa
    49621 tagcttctca tattttctag gctagtgagc ttattaccga gaagcagctg attgaacacc
    49681 gtttaccgag aggtcatgaa aaagctactt tgtttcgagt attaagaaaa attctgtttc
    49741 aaaacaagga gatgcaacaa aacttatgtc gcatcctctc tccttagaac tttcacttat
    49801 gtttttcttg taaaattcat aaatattttc atgaattttc taatttcttg cctacctctc
    49861 gcataggata tatgatgaat ttatctaaag tttgcaaagg agttcgacaa ttgaaaaaat
    49921 atggcccatt tttagcaatt tttttcattg ctttggcatt tctcatgcct catttattca
    49981 cacataaact aattttgggt gctgatgccc cattccatta caatcggttc tatgatgccg
    50041 cgcaacaaat taaatatggg gatttccatt attttccttc cctttatgga ttccagcaaa
    50101 gtggcaggat cgtgaatgcc gtttacggcc catttttcgc ttatttacaa ggattgatcg
    50161 tgttgcttgc aggtagctgg tttcattatc agattctttc aaatttcatt ctttatctta
    50221 tttcaggatt ttctatgctg attttactga gatatgtcaa tgttcgaaat tgggtaagtg
    50281 taccagtagc cgttttattc atgagtacgt attctattca atactggtct atcaaccaag
    50341 gattcactag ctgggggact tgtttttatc ctttatgtat gatccctatt gctgatttcg
    50401 tccttcataa aaagttccct gttatcaaag tcgctgtctc agtgggactt atgacacaga
    50461 tccatttact gtcttcgatc atgttagctt tgatgtatat ccctttttat ctttattact
    50521 tttttaacca gactgaaaaa ggaaaagcac tgcttcaact gattaagtcg gtcttgcttt
    50581 attttgttct gacagcaaat atttgggcag gattagctat tgtttacagt gggaataaga
    50641 ttcttcctcc ctttgttaac agagaaatga gtcaaaaggc gatagatctg gatggcagtt
    50701 attggctcca ttaccctaag gcctttccta tattgatatt atcaggtttt ttgcttttta
    50761 ttttctttca acaaaagaac accttattcc aaaaaatcgt gttgtacaca agtcttgttt
    50821 tcttttttct ttctactagc ttgattccat ggacgacatt gatcgaaaaa aatgtgcctt
    50881 ttattagttt gatccagttt ccatttcgat ttttcgtgcc atttacgcta ttgcttctac
    50941 tttaccttgc cctttcactg aaccagtggt ctgaacaaaa gtggttccgt cttttaagta
    51001 tatttggcat tcttattttt tcagcacaga cgatccagaa cttataccag catctggaaa
    51061 agtgggaaaa tgaaacattt gtcagtcgac atacttatct attcgatacg ccggaagaag
    51121 caaggaaaac attcttcagc aaagatatgt ctaccagctt gttcgttttc caaaaaacca
    51181 cacctgacta tctgccaatc tataaggaga caaaagacaa taaatacgac cggtataacg
    51241 aagaaatact agaaaacgaa acaactttta taaaaacaca tccaggcggt cttttgacaa
    51301 tcaagtggaa aaacactggg gacaaaactg ttcatatccc tgttatcgtc tacgatcgga
    51361 ctgtgctaca gcaaaatgga aaaacattga ctgattatga agtaacagat atcgggaccc
    51421 caatcgtgaa gcagcaaaaa ggaatcaacg agctcacgct tcattatcaa acgccaatct
    51481 atttctattt cattctttct cttactttga ttggctggtt taccttgcta tgtttattta
    51541 tttatcatag atacaagctt ttacgtgctt aggcaaaagg gataaataca tccgatcaaa
    51601 aaactctcca aacggtataa tcgtttggag agttttattt taatcagttt ttttatgtta
    51661 tatgttatat tttttattcg ttcacatcta attcgatttc caccccgtag tgatcactga
    51721 tgattggact atcgatccca tcaaaaatcc gttgatattt tttaacctgc attccttcag
    51781 ggacgaaaat aaaatctata cgcaattttt ctgtattttg ttcccaacca tctattttct
    51841 tttccacagt agctgctttt cctttttcat tcgcgacaac atacgaatct tgaagaagaa
    51901 ggcaactttc tcttacagta taataacctt gagagtctac tggattattg aaatcaccta
    51961 ggaaaaaaag tggtacgcga gtttccaaaa agtagttttc caagtttttc cactcaaaat
    52021 aaaagccact ttttttatct gtccaccaag aaaagtggca gctcgagaca aaaatgtcct
    52081 gatcatctat ctttgttttc ccaatcaaga tcttgcgtgt ctgataatct tctttctttt
    52141 gaaattggga gacggtaagg acctgacttt ccaatgtttt tttcgtaagc agtgcatttc
    52201 cttcttcata tttatcataa ccgatgtggc tcatttccca actccaaaaa tactcttttc
    52261 cgtgttcctt taaaagctga actaaacggt aggcaaaatt atctttacgg ataggtactt
    52321 gattctttac cggacagaac ttcattagct tcgcaggttc tacttcttca gattccagta
    52381 attggttgat ttcttgtaaa gcaatcactt cataatcttc taacaggatc ttatctgcta
    52441 attcttgcaa ttttttctct ggttctggct caagccagct atgcgtgttg agtgtcatga
    52501 atttcattgg ttttctcctt gtcttcattt cgaaacaaaa gggttgtgac aaaatttttg
    52561 tcacaacctc tccagcaaat agtgttctcc tgctaactcc tcagtaataa gctcaatgga
    52621 ccaggagcta ttttttgttt ggctgttttt atcacttggt gcagggcagc tttttcacga
    52681 cctctccact aaacagtatt caaactgctg cttctcggta ataagcccaa ccaaacgaaa
    52741 aataggagga gctatttttg tttggctgtt cttatgactt gatgcaggac agctttttca
    52801 caacctctcc ttacatttta atggagccga tcactttacc agctgtcact gtacctaatt
    52861 gcttgatcac tacttcttct actttatcgt ttgtaaatac gatcatgatc gttggatctt
    52921 tttcttcgtt ttggatcaca tcaagatcca tctcagctaa tttcgttcct gttcctactt
    52981 cttggccttc tttgacagca atctcaaaag ctggctgatt catttggact gtatcgattc
    53041 ccatatggat caaaacttcg ataccatctt tcgtttccag cccaattgca tgtttgctag
    53101 ggaaaatact tgtcactttt ccagcgatcg gcgcaacaac ttctcttgat gttggaataa
    53161 cagcaaagcc gtcacccatc attttttgtg agaataccgg atcttttact tcagataaag
    53221 gaatcacttc tccagaagct gcggaaacta gttcttgttc tatacctaag tattgcttac
    53281 tacctgcttg tccatttcct gtagaaacca tttctggctc tggaatctct gctcctgatt
    53341 gcagcagatc ttcgatatct gatttcaaga catctgcttt tggtccataa actgcttgga
    53401 ctccattgtc tttgacgatc aatcccatcg cccctgctcg tttccaggct tcttcagtcc
    53461 ctactttttc tcgatcattt acacttacgc gtaaccgagt catacaagca tcgacatctt
    53521 tgatattttc ttttcctccc agcaaatgaa tgatctggac cacttgctga tctgctgttc
    53581 ctgttccgct gttagataca acggtttctt cactgtcatt gtcgtaattt ccgttacgtc
    53641 ccggtgtagc aaagttgaat tttttgatca agaaatttgc taagaaacac gtaacaacac
    53701 caaaagcgat cacacacaac acgaagttga taagatctcc acctagaccc gctttgatag
    53761 ctaacggtgt ccgagtcagc aattcgatat tcccaaatga atggacccgc aatggcaaga
    53821 tatctgccat cgcaaatgca gccccttgga tcactgaata aatgacatat aatggcacag
    53881 cagcaaacat aaacatgaac tccaatggtt ctgttacacc tgttagaaag acagccaacg
    53941 ctgctgaaaa atacattgat ttatatcttg ctttcttatc aggatcgaca ttacgataca
    54001 tcgcaaaagc cattcccatc aagatacctg aagaaccaat catttgtcct actttgaaac
    54061 gagccggcgt ccagttatgc aacacaaact catattgcga catatcccct gcacctttaa
    54121 gattcactaa atcagtggcc caagccaacc ataacggatc ttgcccaaat acttgtgtcc
    54181 ccgcctgtgc gcctgataaa atctcatacg atccgccaag ctgtgtatag ttgataggaa
    54241 tcgtcagcat atgatgcaat ccaaatggca atagtaaacg ttctaatgtt ccaaataaaa
    54301 acggtgctag aatcggtgcg ctttcttgtg attctgcaat ccataaacca aaattattga
    54361 ttccagcctg tacgtttggc cacaccacag caagaatcaa tgccacgatt gttgaccata
    54421 agataacaac aaacggaaca aagcgctttc cattgaaaaa tgacaaagca tccggcaatt
    54481 ttctataatt atagtactta ttataagcca ttgccccgac aaatccagcg atgatcccta
    54541 cgaatactcc catattcaaa gctggtgctt ccaatacact tgtaaaaaat cctttaacca
    54601 tgatcttcgt accgaacaga gtgtgggtaa atgcatcttc attcgcaagc atgtcacttg
    54661 tgacaccaaa gatagcccca gtgatccgat tgattaaaat aaatgagatc ccagctgcaa
    54721 atgctccgcc tgctctttct tttgcccaac ttcctcctat tgctaaagca aataaaatat
    54781 gaagattacc aataattgcc caaccaatat tttcaattac tccgcccgtt gtgacaagcg
    54841 gcgtccaatc aggattgatc aagggaattg atttccctat actgatcatc aacccagctg
    54901 ccggcataac tgctaccaca accattaaag cctttccaaa tttttgccaa aactcaaaac
    54961 taaatatctt tttcattccg tttagctcct ttacgcaatc ggtttcgtta aacgtattat
    55021 agcgtgcgtt ttcttttttg taaatgcttt attttttatt tttctgtcta taccattcta
    55081 taaaacctat aatacgtgtg ttcccttcta taattttctc ctaaaaattt atttttttaa
    55141 gcttttttct atgaaatatt ttcttttgtc tattgacttg tgcaatcggt tgcgtttatt
    55201 atgaacgtat aaaacagctc attaggagga gaaaaatgaa acagatcaaa cgactatttg
    55261 ctattgatcc atggaaaatc agcacgacac aattagataa aaaaaatctt cgactgcaag
    55321 aatctttaac aagtattgga aacggttata tggggatgcg cgggaacttt gaagaaactt
    55381 actcagggga ccaccatcaa ggtacttatt tggcgggtgt ctggtatccc gataagacac
    55441 gtgtcggctg gtggaaaaat ggttatccag aatactttgg aaaagtgatc aacgccttga
    55501 actttatcgc gatagatatt tcgatcaacg atcagctgat cgacttggct tctttggaac
    55561 cagaagactt ttactgggaa ttagatatga aaaatggtgt attatctcgt acattcacaa
    55621 tcactacttc tactaataaa gtacgcattt ccttcgaacg ttttctgagc attgtcaaaa
    55681 aagaatcagc ttatatctat ttaactatag aaatgcttga aggtagcgga acagtcaaac
    55741 tcgtatcaaa attggatgga aatgttcaaa acgaagacag caactatgat gaacattttt
    55801 ggaatgagat tgatcgtgga actatagaag aaatcggcta tttgacgact aaaactattc
    55861 caaacaactt tgggattgaa caatttactg ttactgcagc aatgcgccat ttcgttaatg
    55921 gaaaatcagt catccctgac tatcacgaag atactttagc attacatggt tgcttgtctg
    55981 cagaattatc tcaaggtgag acatggtcag tggaaaaaga aattatcgtt cttactagta
    56041 gagatattcc agaagcgcag caagcagaaa aagctgtctc agaattaacg aaccagacaa
    56101 acaattatat ccaagcaaaa gaagagcaaa cagcggcttg ggcagaacga tggaagcttg
    56161 ccgatgtggt catcgaagga gacgaagaag cacaacaagg tattcgattc aatttgttcc
    56221 aactattctc tacttactac ggcgaagatg aacgattgaa tatcggcccc aaaggattca
    56281 caggagaaaa atacggcgga gccacttact gggatacaga agcgtatgct gtccctcttt
    56341 atcttgcttt agccaaacct gaagtaacta agaatctgct gaaatatcgg cataatcaac
    56401 tgccgcaagc aatccacaac gctcagcaac aaggattaaa aggcgcgctt tatcctatgg
    56461 tcaccttcac tggtgtcgaa tgccataacg agtgggaaat cacattcgag gagattcacc
    56521 gaaatggcgc gattgcctat gcaatctaca attataccaa ttacaccggc gatacggaat
    56581 atttgaaaaa tgaaggactg gaagttttag cagaaatcgc acgcttctgg gcagaccgtg
    56641 tccatttttc aaaacgagca aataaataca tgatccacgg tgtgaccggc ccaaacgaat
    56701 atgagaacaa tatcaataac aattggtata caaatacgat cgcagcttgg gtcttgcgtt
    56761 acacgcgaga aaattattta acttatcaaa aagaagctgc tatcactatc agcgaagaag
    56821 aactagccaa atggaaagat atcgcagaga acatgtactt cccagttgac gaagaattag
    56881 gaatctttgt ccagcatgac acattcttag ataaagatct catgcctgta tccgatattc
    56941 ccacaagcga tcttcctctg aatcagcatt ggtcttggga caaaatcctt agatcttgct
    57001 tcatcaagca ggcagatgta ctacagggga tttatttctt caatgatact tttacaaaag
    57061 aagaaaagca aaaaaacttt gatttctatg aaccaatgac tgtccatgag tcttcccttt
    57121 cgccaagtat ccattcgatt ttagcagcag aattaggaat ggaaagcaaa gctgtagaaa
    57181 tgtaccagcg aacagctcgc ttggatttgg ataattacaa taacgacaca gaagacggct
    57241 tgcacatcac ttccatgact ggtagctggt tgtcgattgt ccaaggattt gctcaaatga
    57301 aaactgccca tgatcagttg agattcgcac catttctgcc aaaaacttgg aaagcttatt
    57361 ctttccatat caattatcgc ggacgtttat tgttcatctc agtcacagat gaacaagtta
    57421 ctttcgagtt attagaaggt tctcctctag aaatttacgt ctatgaacag tcttattctt
    57481 tagcggatcg tctagaaata acgctaagaa aggaggaagc aaatgtttaa aggggtattg
    57541 tttgatttag acggtgtcat taccgatact gctgaattcc attatcgtgc ttggcgaaag
    57601 cttggtcagg aaatcggcat tacgatcgac cacacattca acgaacagct aaaaggcgtc
    57661 agccgagaag actcactctc tttgctttta gcttatggcg gaaaagaaca ctctttttca
    57721 aaggaagaat ttgcagaatt agcgaaaaga aaaaacgatt attatttaga gatgatccag
    57781 acgatcgaac caaaagatgt cttccccggg gtcgtgcctt tactggattc tttaaaagaa
    57841 gaaaaaatca agattgctct tgcttctgca agtaaaaacg ggccattcct acttgaaaaa
    57901 atgggtctta caccttactt tgatgctatt gccgacccgg cagaagcaac aaacggaaaa
    57961 cctgcgccag atatcttcct ccttgcagca aaagctgtcg gactaaaagc agaagaatgt
    58021 atcgggatcg aagacgcgca agccggtatc caagccatcc tctcaagtgg cgctcaacca
    58081 attggcgtgg gacgtgctga agaccttgga gaggagatcc cgcttgtccc tgatacgact
    58141 tttcttacga tcgattactt gaaagaaaag tggcatgacc atggataatc cagagatctc
    58201 cgtcgtacca gttccagaga ctgaaataaa gaaaatcaca ttgaagaacg actacttaga
    58261 agtcgttctt ctcaattacg gtgcccgcct gcaccaaatc ttcgcaccag acaaagaagg
    58321 aaaatcagaa aatgttttat tgtcttacga tcgtttcgaa gatgttctaa cagacaaaag
    58381 cttttttgga gcaactgtcg gacctgtagc cggaaggatc cgggatgctt cttggggtga
    58441 gcatagattg gagaaaaact gcggatcaca ccatatccac ggtggcacga aaggctggtc
    58501 tttccaattt tgggaagtag aagttttcaa aactccatca tcaataggtg tggtctttta
    58561 cttaaaagat gaattttcta cctatccagg accaatcact gctacgatca cttaccgttt
    58621 aacaaaaaac gaattagaga tgacaacctc tgctagtagc cttgttgaaa ccatctgcaa
    58681 tccgaccaac catgcctact tcaacctttc aggaaatgga acaagagaca tctatgaaca
    58741 tcagttaacg gtctttttag atggtgtgct tgaattagat caagaaaaac tgcctactgg
    58801 aaattggaca aaaaaagaaa aactgccaat cgactttaga aaatccccaa cactgcaaga
    58861 gatccttgca tgttatccag atggactaga tgatgtattt cttttgcatc atcctaggct
    58921 ttccaaaact tccttacaat tattcgagaa gcattctggt cgccaaatga ctatagcaac
    58981 ttctaataaa agtatggtat tattttctac gactggattc gaagctgatt tctctgtcaa
    59041 tggaaaacag atgcattcaa actacggatt agcaatcgaa ccgcaagaaa tccctgatat
    59101 cgttcatttc cccaaatttg gatcgatcaa cctccatcct ggtcaagaac ggatcagtca
    59161 gacgatttat cggttctctg cacaataaca agagaaaggt gtttttatca tggctattac
    59221 cgtaaaagat gtggcgaaaa aagccggtgt tgccacctcg accgtctctc gtgtcatcaa
    59281 cgatcatcct agtatttccg agacaacaaa gaagaaagtc cgaaaaatta tggatgaact
    59341 aggttatgtt cctaacatga ctgcccgcaa tctaggaaaa cgcatttcca gtgccatcgg
    59401 cgtaatctta ccccctcttg attcaaaaga gcggatcggt aacccatttt atcttgagat
    59461 catggaagca atcaacgaag aagcgcggaa ttatgatatg actacagcaa tcgctactgc
    59521 taaaaatttt gaattgctgt tagagaacgt ccagcgtatg catttacaaa aacaagtaga
    59581 tggttttatt ctagtgtatt cagatagtca agatactgtc attgattatt tataccaaaa
    59641 ccacatccct ttcactttga tcggacagcc ttatcatcat gagaacgaaa tcgtttatgt
    59701 agataatgat aaccagcttc taggtaaaca agcaacagaa tttttgatcc agaatggaca
    59761 taagaatatc ctgtttgtga caaacacgac tcatgaaagt ttgtatttcg aacgatattt
    59821 cggttatcaa aaagcaatga tgatggctgg tcttcctgcg ttatccagtg tagtactaga
    59881 gcatccggag gattatctgt cctttgagaa aacgttgaaa gaaaacgaag ccacagcatt
    59941 ggtggtcatt gacgatatct tcgcccttcg cacgatgcag ttggcgcaaa tgtatggcta
    60001 tgtcattcca gagacactct ccgtcattag cttcaacaac tcgatcttct caacattaac
    60061 acacccttac cttacaagta tcgatatcga tatctccgaa ttaggacgtg tcgctatgca
    60121 aaagctgcgc gaattgatcc atgaagaatt gagcagtggc gtacgtttgg tcatccctca
    60181 tcgcttgatc aaaagagaga cagtcgttcc tttagaaaac ccatactaaa tagctgactc
    60241 actaaatacg ctgaccattt atcctatggt cagcttttta tgtaaaagaa gatactactt
    60301 ctaacacatt gtttatctgt aaaaaagagc gtgggacaaa agtaaagaac acttttgtct
    60361 cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcggaa ataagctgaa
    60421 attcacaaaa atttaaaaag caattttctt gagtttcttc ttatttcttg gagttaaacg
    60481 cttctgtccc agcctctttt ttccttcctt caatttttta cacgtcgacg attcaataaa
    60541 aaaccgccta atattgtaaa gacaggtgca agaatcgaga aaaaggcgaa tggcaagtaa
    60601 tgcaacgcgc taactttcat cgttcccata atgaatgtgc cgctgactcc ccacggtacg
    60661 agagaattga cggtcgcacc tgaatccgct agtgttcgtg tcaaataggt ttttgataag
    60721 ccaacgtcat caaaagaacg cttgaatgtt tctcctggaa gaatgatgga cagatactgt
    60781 tctccaacga tcagattgat tccgatacag ctcaaggcag taaacccgac taaacgagaa
    60841 ggtcggtcca tcttttcttt taattcttga acgatggtat caacgatcag atatttgatc
    60901 agtaacccac ctaatgccaa tgcaagtaaa atcaaactag cagaacctaa catactcatg
    60961 atcccgcctc gagaaagcag ttcatctagc tttggatcag gtgtatttgc gacataacca
    61021 ttcatcaaga tgtctgctgc ttttccgacc gtcaaacttt tctcatggat aaaagaaaga
    61081 acgagagcag ctgtcgatcc cgctagtaat gtagggatag ccggaatttt tttgattgcc
    61141 cccccaaata aaataactag tggaatcaat gtccaaggag agatccaaaa gctcgctttc
    61201 atgttttgga tcatttcatt gatactagct agatcggctg aatgactgtt atgtcctaat
    61261 atcgtataaa aaatcaacga aaggacgaat gctgggaaat ccgtataacg cataccagcg
    61321 atatgttcga atagattaac tcctccaata cccgctgcca agttggtcgt atctgataaa
    61381 ggcgagcagt tattccctaa aaaggcaccg gaaacgactg cgcctgtagt cattgcatca
    61441 ttgaatccaa gtaaatgacc gatccccaaa aaagcaattc caatcgtcga aatcgttgta
    61501 aatgaactgc cgacagtcac tccgacaatt gcacaaacaa gaaatgctgt cggtaaaaag
    61561 aaacgcacag aaacaagttt aaatccataa accataatcg tagggatggt tccagataaa
    61621 atccatgaag caactaagac accaatcaaa agaaaaatca ggattggaat gatccctgga
    61681 gtgattcctt tgatgatccc gtcatggatc tcatcccatg tgaacttttt aaatctgcca
    61741 taaaacaaca aacacatgaa agctgcaagg atcgggatat gtggcgagag ctggaatccg
    61801 atgatcagta ctcctaggag tgccaacaac acgatcaata cagcgatact ttcttttaaa
    61861 gaaatatttt tattcatcgt cgatctccta tttcaaatga aaaggccctg ccatgaaaaa
    61921 actattcact tggcaggacc tcctactaaa tcagcgacaa aacgtcggct gaaagactta
    61981 tttttttatt tttcttctaa acttttttct acctcaatcc cccaagaatc tttcttgaag
    62041 tgattgatga tcaatggaat aacgaacatg accactgtga tagctgctac taataatgca
    62101 tcactcaatg tcgctgtacc taatgtagca aatacgacaa cgatgataga gaagatcagc
    62161 atgcagctga ctacccaagc taatccgttt ccgtttttac caattcggta aggacgttcg
    62221 aattctggat gtttcttacg cattttaagc aatgaaacag caatcaaaat gtacacgata
    62281 caataaataa cagttgtagc atttgtaagt gtcaagaata cagcacttac gtcatccatt
    62341 acaccataaa gcaaagcaaa taatgaaata acgattgatt gtgttagaac gacatttcta
    62401 gatacgccgt atttgttttc tttatggaat ccgaattttg gtggaaggaa tccttcacga
    62461 gctacttgga tgattgtttt tgatggtcca gtaacccaag ctgataattg taaaagcaca
    62521 ccaataaata ccatgaagct gaagatattt ccgatcactt ctggtaagcc taggatttga
    62581 cagtcaatca agataggctg cgtgatattt gctaattcca tttttccatc tggtacgaca
    62641 tttgctacaa gcatcgcatt gatgacattc aataaaacta atccgatcaa agcgataaat
    62701 acgccttttg tataattttt tgttgcgtct ttcaaacgag gcatgtaaac agaactcatt
    62761 tcgatcccaa caaaaataaa tgtgatacca gctaagtatt tcaatgaatc aatatgttcc
    62821 aagtcaggga aagcttttga ccatgaaaag tcacctagat agccgtttga gactagtccg
    62881 actttgaagg ctgccaaaac acctaaaacg aacatgatga ctactgggat atatacccct
    62941 agccatacac cgatgttccc gccgactttt gccatatcga atttcaagtt caagatcgtg
    63001 atgatccagt agataactaa aatacaacct aaaacgaacc agtgattatt gccaagttct
    63061 acattaccaa atgtgtttcc taacaatggt cctaaagtag aagcgaccat taccatacct
    63121 gggaacattt gcacccataa tagccatgca acgacaaatc cccatttgct gcccagtgct
    63181 gtttttaccc ataactgagg accgccttct tgaggaagca tcgtggaaag ctcaccggaa
    63241 accattgata tcggaccggc aaaaaacaaa actgcaaaaa ttgaataaaa aatcaatgtc
    63301 caaccgactg cggctaacgt aggaatactc cgaaccgttg cacacagtgc cattgtcaga
    63361 ccgataaagg tcccaagacc cattttactt acttcagatt tactcaccgt tgacactctc
    63421 cttgattttt tggataaaat aacggtgtct ttgaagtagt ttcagcaata aacgaaacaa
    63481 ttcagcgaat atttgagggg attctttgag tttattgctt gaagggtttc ttcaaagaca
    63541 acctcaaaac aaagggatcg aagagcttag gtctcccctt gctcttcaga tccaaacatg
    63601 cgtcagaaac agttagtatt caagctgttt tctaacatga ttaccttgct tattttgctt
    63661 cgcttgccaa taattggtct gcgtagattt tttccaattt ttcttgaaga gctgctttga
    63721 ttttttcagc atattcttca aagtgttctt ctttgttcat gtatggtgtc attacgctgg
    63781 cacgcaatac agttactttt ccggcacggt tccattcttc atctgagaat cctagttggt
    63841 tcacaaattg taatgggctg ttgccatagt ctgggatagc aaagtcagta tgtgaagtta
    63901 agaactcatt accgtaaatg cttcctttca cgtatgaaga ataatcatat acgtcatggt
    63961 tcaatttatt cattgcaact aagtcatcgt tgcctttttc tttgaataca tagtctacca
    64021 tattgaaatc tggataagtt aatggatgaa cttcgatttc tttgtctcct actttaaatg
    64081 acaagtcatt caagaagttg tagaaacgat gtgcaccttc gattgatgca cccattaatt
    64141 taccatatcc tgtgacattc aaaggtaaga catggtgtgc tgcccaaacg ctggctgctg
    64201 ttgcccctgc ttttgaacct tccaagatat aagccccaag taatgctggg atatctgcgc
    64261 ctttttcaaa tacatatgtc gcaaagtaag agataacgtt tctcatgcgg atatctttga
    64321 tgacgatacc accggcagaa tatggaacat agcccatttt atgtgggtca atcgttacag
    64381 attctgcttc ttcgattgct ttatatgcac tgtgtacttc ttctaagata taatctttgt
    64441 tttctgtaaa gacattgtat ttgtaatgaa catcttttaa atcttcaaat ggaatgaagt
    64501 tgttgtcttc gtctaagaaa atcgcacggc cgtatccgcc ataagcagca tctacgtgta
    64561 agtagaagta aataccgtct ttttctaata cgcgacgcaa tgcaacgatt ttatcgatac
    64621 cgtcgattgc tccttcttct gttgatccta caacaccgac tacacctagg attggtgttt
    64681 tttcagctgc tagaccacga acgatttttt caagttcgtt gatgtccatg cgatagttgt
    64741 ggtcaactgg cactggaatc acttggtcta aaccgatacc gatgatgtcg gcagctttta
    64801 gccatgaata gtgtttagtt tgtggaacca accattttcc taatttttct aaatgttttc
    64861 cgctacgtgc tgagtgagct ttgatttcgt cgattttttc tgggacgctg tctaacagat
    64921 tcatgatttc ttctgttgac aagttcatta attcccaatc gctttttcca gcaactagtt
    64981 caggtgtcac ttctttcatt gcaagcggca atgatttgat gttgcgggca taccaaagtc
    65041 cttctaagtt tgctaaagaa ccatcagcaa cgatgtggcc ccagccatct ttatagctca
    65101 ttaattttgc aaattccatt cctacttctt cttccatttg gcttgttgca ggagaagatt
    65161 cataggcaac gttattacca ttccatagca tagcaaaatt gtacgctagt aaagatggca
    65221 tcaatgtttc agagttcata tgaccccagt aacgtccggc attatgccaa ggaacggaat
    65281 gggtacgcat ccgcgctgaa atttctgata gaacatcctt tgttttattc acagtgtgtt
    65341 caaaactagc actgcttttt tcttctggcg taataatagg catgtcttga ggcatgtagt
    65401 tttgacgcca gcctaaatgt tcatccacca attcattcaa caaagcttta tagatttggc
    65461 cgttctcagc tttgtcccca atgaataggg catttaggtt tagatctttc gacaatgatt
    65521 cactcatagt gaaaccctcc taaaaaaata tttaaatttt gtcgagccct acattacacc
    65581 tgttcttcta ttctgtaaac taagttttgg cctgattttt aatcatattt taatatgtat
    65641 caattatccg aattgttcac acttttgaaa taattaaaaa aaacgaggga atttacaaat
    65701 gtaaactccc tcgcaaagat tgtattaacg acaaccagcg ttctatttta ggatgggttt
    65761 caaaattgga atgttgtaat agtatttccc tttttcaagg taaaaactta ctaaattcta
    65821 atcaattact ttggccaaaa agtaattttt cttacctttt cgaataacca caaacttacc
    65881 atcgaattca tcggatggat ttatgacaaa atttaaatcg gtaacgcggt caccgttaat
    65941 acttatagct ccgtttgaaa catcttcacg tgcttgacgt ttagatggtt caatttttgt
    66001 ggaaacaagc agttccacga tattttcagg tgtgcttgaa atttcaacgt tcggcatttt
    66061 accgaatcct tgcgcgattt cttccgcatt tagttcttta atatttccag agaacaatgc
    66121 ttgcgttatt ttttgcgctt ctttcaagtc ttcttcacta tgcacaaatc ttgtaacttc
    66181 ttctgccaaa cggcgttgtg cttcacgttt ctcaggttct gtctctactt ttttcgctaa
    66241 gtcttcgatt tcttcttgag acaagaaagt aaagaatttc aagtatttca ctacatcgcg
    66301 atcatcttga ttcaaccaga attggtagaa ctcaaatggt gaagttttct tcggatcaag
    66361 ccaaacggca ccgcctgcag tttttccgaa tttcgttccg tcagccttca gcatcaatgg
    66421 gatcgttaaa ccgaacgctt ctgcttctgg tccttctaat tttcggatta ggtctaatcc
    66481 agctgtgata tttccccatt ggtctgctcc accgatttgc aattgcacat cgtatgtttt
    66541 atgcagatgc aaaaagtcga tagattgcag gatttggtaa gtaaactctg taaatgagat
    66601 tcctacttcc aaacggctgg caacgatatc ttttgctaac atcgtgttga tgttgaagtt
    66661 tttaccgtag tctctcaaaa attcaagtaa agagattttt gacaaccagt cgtagttgtt
    66721 cacaaatgtg atatttgcat ctttaccaaa caattttctc atttgattgg aaagtgcgtc
    66781 cacgttatgt tgcacttgtt ccatcgtttg caacacacgt tctgttttgc ggccgcttgg
    66841 gtcgccgatc gttcctgttc cgccgccaat cagaatatat ggatgatggc ctgccaattg
    66901 gaatcttttc atcatcataa atggaatcaa atgtccgata tgcatcgaat caccagttgg
    66961 atcaacgccg caataaagtg aaatgctctt ttcttcaacg agctcgcgaa gtctttcttc
    67021 gtttgtttgc tgattaatgg cgtcgcgcca agttaattca tcaataatat tcattttact
    67081 gacctccgtg tagttttttt atgaaagcag gaacctgcta gcatatgtaa ttaattattt
    67141 cttcaggata ggataggaaa taaaaaaagt cccttggcat ataagccaag ggacgaaatc
    67201 tcgcggtacc acccaatttg tatgtctaga tatccaacat acctctcgaa catttaacgc
    67261 tgtcgcgtgg caatcactgc caaagactcc tgaaatgtaa ttcgctttaa agcatgtact
    67321 ggtttgcagc gaccaccagc tttctgaaac attggcttta acaactactt cgtttcgatc
    67381 ttcgtcttgt tattattaat atgtgtctat catagagcgc aactttttct ttgtcaacag
    67441 aaaaaggata ttttttttaa attcctcatt ttttcttttt tgaatccgct ctaattccga
    67501 acattttatt ttcaattatt gttaaaagaa ataacttttt ctgtattttt tctgcaattt
    67561 atcgagaagt cactttactt tgcctcttat tctttcttcg ttacaataag gaatgggaca
    67621 agtaaaaaaa tgaaaaagca agggacgagg tatatggaaa aaaagcaaaa aattatttta
    67681 ggagccactt ctgctttaac tatcgttatt ttaattctaa gtttgttttt tgtcatccga
    67741 tcatctacaa aaaaagaacc aatcattcct gaaaaaaaaa taacagaaaa agcaaaagaa
    67801 ccgacagatg ataagcaaca actagttact caagcaaaaa atctggctca aagctacgat
    67861 tatgaccaag cgattgcgtt attggaaaaa gatagtaaga acggatctac tgaaacacaa
    67921 cagcttcttc aaacactaaa aaaggagaaa gaacaactga tcgactggtc tgatcctact
    67981 cagatctctc atgtcttttt ccacagcttg atcgtggacc cgcaaaaagc cttccactct
    68041 caacaggcac aaggatacaa agattatatg gtgactgttg aagaattcaa ccgttcgatc
    68101 gaacaacttt atcaaaatga ttttgtttta gttaatttga atgatttgat ccaaaaagac
    68161 gaacaaggaa atctttcctt taacgggctt gcgctacctg aaggaaaaaa accattggtt
    68221 ctttcacaag atgatgtcag ctattatgaa tatatggata attccggttt tcctagccga
    68281 ttggtcgtat caaataacaa tgaaatcaaa aatgtctata aagagaaaga taaagaagaa
    68341 atcggtgact ttgacatggt tcctctgatc gatgcctttg tcaagaaaca tcctgatttt
    68401 tcttatcgag gcgcaaaagg taccttggcc ctgacaggtt acaacggagt tttaggttat
    68461 cgaacatcaa aatctcaata tggagacaat gaaaaaacaa aaaaagagat taaagaagct
    68521 aaaaaagtgg cggaacagtt gaagaaagac ggctggagct ttgcttccca tacatggggt
    68581 cacttgaata tgactcaagc ttcactagaa gatgtaaaaa aagacaatga attgtggcag
    68641 caagaagttg cacctatcct aggaaaaaca aatattctga tttatccatt tggtgcagac
    68701 atcagcgact ggcagcctta ttcagcagaa aatcaaaaat ttgcttactt gaagagtcaa
    68761 ggtttcgata ttttctgtaa tgtcgatgct tctacaccag cttggggaca attaggtacg
    68821 gatttctatc gaaatgctcg gatcaatatc gatggcattc gttttcaatc tgatttagac
    68881 ggaaaaaatc ctattcttga tccattcatc aatgtccatg atgtctatga caaagaagca
    68941 cgcgaatctg cttgatttta cgtcatttat gaaccaaaaa agccgaactg acattttatc
    69001 gtcagtttcg gcttttttat gctttatttc ttcttcggtg cgattccctt gatcaggact
    69061 tctgtccatt ctgctgtata tggaagcagt tttgcgtata tctccggctt agaaattcct
    69121 tctaccattg cttgaaaata gaggatcaag aactcatccg aatatttcaa atccactttt
    69181 ccttctttcc gccctcgatg gaaaagatca agcatgatac tgaaactttc ttgcgtatac
    69241 gcatccatcg cgtcagaaaa ttcttttttt tctttttttg tatagtatgt catcaaatcg
    69301 atataaaatt gtttgttcac tcgattgaga taacggattt tgttttcact catggcaatc
    69361 agtgtttctt caaatggaag attttccgca atcaacgctt tcgcttcttg ccctaaatca
    69421 ttcataaatg caaaaaatac ttcataaatc agcccttcct tgctgccaaa atatttaaaa
    69481 atcgtcgctt tacttacctg agcgttctca gctaccttat ccattgtcac tccagcggtt
    69541 cctatgtctg tgttcatcaa gtcaaaagct gcttgtaaaa tctctttctt tttctcttgt
    69601 gctctctttt caaaaccgtt catttgtaga gcctcccgta attttaaact tttataatat
    69661 atatagttca aatttattct gaggtttatt ataactatct attgagaaag tatcaagcta
    69721 gcaattcctt gtttatgaat tcattgacag aaatttataa aaagaatatt atgtactaaa
    69781 ataataaaaa aagttttata aaaaggtata aaaccttttt attaaactat ttaatcttgt
    69841 ttagtatata tttttagaaa aaggagtggg gtttttatgg cagaaatcgt caaagtcaca
    69901 catttgaaaa agagctttgg tcgtttcgaa gctttaaaag atgtctcttt caccgtacat
    69961 tcaggtgaag tcgttggatt tatcggacca aatggttctg gaaaatccac gacgattcgg
    70021 acacttttag ggattataaa aaaagacggc ggaacggcaa cgatttttgg gcaagatgta
    70081 tgggaagata gtttatctat ccatgaacgt ctttcttatg ttccaggcga tatctctttg
    70141 tggggaaatt tgactggagg agcgatcatc gatctcttca tgaagcttca tggaaaaggg
    70201 agtgctcaaa aaaaagatga actgattcaa cgtttctctc ttgatcctaa gaaaaaagca
    70261 aaaaactatt caaaaggaaa tcgccaaaaa gtgggcttga ttgctgcttt ggctgttgat
    70321 tcggatctct atttatttga tgagcctact tctggtttag atcctttgat ggaaacgatt
    70381 ttccaagaag aagttgaaaa attgaaagaa cagggcaaat ccatcctact ttcttctcat
    70441 attctcagtg aagtcgagcg actagcggat aaagtcgtga tcatacgcga aggaacactc
    70501 gatgaagaag gaacactcga tgaattgcgt catttgactc gttcgaccat cactatagaa
    70561 acagaagcag agaatagtca cttagcacaa ttgaacggtg tccatgactt tgtgcaaaaa
    70621 ggtcacctcg ctcaatttgc tgctgataac gaagctttcg atgtgatttt agctgaagcg
    70681 tccaaattag gtgtgaaaaa atttgaatct attccaccga ctcttgagga tttattcatg
    70741 agacattatg aaggatgagg aggaagcgaa atgactgata aattttcacg ctggtccacc
    70801 cttttttctc aaaaccttag ccgtgactgg aaaaagatcc tgatatggct agtcggtgtc
    70861 actgcttttt ctggtggttt tgtccctgcg tttgaagaat tgacttccgg acaaggatta
    70921 gctggattat acgaaacctt gcaaaacccg gcaatgattg caatggtcgg accaactcca
    70981 gcttcgaatg ctgctgagta tactttagga gcgatgtatg caaatgaaat gttgcttttt
    71041 tgcagtttgt ttgctgctgt gatctctgtt ttacacgtgg tgagccaaac gagaaaaaaa
    71101 gaagaacttg gtctgattga acttattcgt tcctttcgag tcggacgtca agcaaactct
    71161 ttagctgtca tcgctgaaag tattctgatc aatttgcttt tagcaatatc gatcagctgt
    71221 acgatgattt ttttccaagc ggattcgatc actgcagaag gtgcttggct ttttgggcta
    71281 tctgtaggta gtatggggat tttggggagt gccgttgcac tgtttgctgc acaactgatg
    71341 ccaagttcag ccagttcgaa tggtctgtct ttagcgtttt taggtggact ttaccttcta
    71401 cgagcaataa cagatgtagc tgattcctcc ctttcgatgt ggaatccttt aggctggtct
    71461 tatttgacct atccatttac aactaacaac tggattcctt tgctttattt gtttttgttc
    71521 agtctaatct tgataatcag ttcatttgtt ttagaggaac atcgagattt gaacaccggc
    71581 tatatccccg aacgtgaagg aaaaccgtat gctaaacgtt ctcttttatc cttgcgtggt
    71641 ttattattct atctaaataa agggatgaca atcagttggc ttatcacttt tggtttgatg
    71701 ggggcggcat acggctctat ctacgggaat atgcaacagt ttttagaaag taacgactta
    71761 atgaaacaga tgtttatcca atctggcgtc tcaattgaaa aaaactttac aagcacgatc
    71821 atggtagtct tgatttgtct agctgctatt ttaccaattg ctagcatcaa tcgtttattt
    71881 acagaagaaa cccacggacg tatgaatcag ctgttcgcaa caaaaatttc taggaaccaa
    71941 ctttattgga ccaatatgtt actagctatt ctatttgcta tattggcaat cctgattagt
    72001 tctggtgcac tagggctttc tgctttggct tcgatgaagg atgcgaaaga catggatcta
    72061 ttgatgtttc ttaaagccgg gttcaatttc ctgcctgccg ttttagtgtt tatcggaatt
    72121 agtggctttc tattcggatg gcttcctcga atcggtaaac ttgcttatat ctacttagct
    72181 tactcctttg cactcaatta ctttggcgga attttggatt taccagaatg gttttccaaa
    72241 acagctgtcc ctagctgggt tccacggatg ccaaacgatt cttttgatct ttccgtgttc
    72301 cttacgttat catgtatcag ccttgcactg ttcgtccttg gttctattgg ttataggcga
    72361 agagacttac tagaaggata ataaaaaagc atgtacaatt tcatgctagc ctccaaatct
    72421 gggtcaaaaa tccaacattt ggaggtcatt tttttattta caagtcttct aactgctctt
    72481 tatcaatgat ccagacttct cgattctttc tttttacata accattctcc tctaataatt
    72541 taaattttct agataaagtt tcgggggtcg taccgataaa tgcagcaagt tctttcattt
    72601 tcattggcag gaaaaagtga tccttccctg tcgctttagc taagtcaagc agatagacag
    72661 cgactcgttc ttctactttt tccatcatca aaaaagttgt ctgctgctgt acttttgtta
    72721 atttctcaac gttgattgct aaaagtttca gactaagggc gggataatgc agtaagagtt
    72781 ccgagaaatc tttttgtctc aatatgcaaa tttccgtttt ttctagtgct tcgccaaata
    72841 aagactcatt tggaacgcct agcaactggc tctctccctc atatccgccc ggctctatta
    72901 ctcgtaaaag ctgttccttt ccgctagcag ataattgata cactttcata ctgccggagg
    72961 ccacgatcac taattgaggt tctccattcg gcgcaagaat ctgttctcct ttttccacat
    73021 agtgatgttt gacaagtgcg tgtacttttt cttgatcttg ctgtactaag tgattgaaca
    73081 aaggaacaaa tgaaacacat aaatgatcat gactcataat ctctcctcct tctaaacagc
    73141 tatttctaat aaagaaacat aacttccctt attccaactg ataattttct tttatcatag
    73201 cataaacgtc aagcgaacca gataccagca aatagagtgg atttcttttt gtcccttgct
    73261 ttattttcct tcttttgaga ccttaagtaa ttgattccta tcaatttttt aaatatttac
    73321 tttttgaata atcaaaaaca aaccttcact cgcaagccga gagtaaggtt tgtttttcgc
    73381 attgcttatt tcgtgttgtc tattgaaatt tttccgcacc tagcttttga tatagtggtg
    73441 ctagttcttg taaggtttca gcggttattt gctcaacgtg ttcgatttct tgcggataga
    73501 agaatagttt acccatttga aaggttcggt ctctcatcgc ttttttcaac tcttcttgtt
    73561 tctttgtatc ggacaaatag tcagacaaag ctaagtggtc gtcaagttca tgttctggtt
    73621 gcggccatat gtaataatcc ttgatatcga ctgttttgct ccactctttc agtatcgcaa
    73681 gcaactggtt atacgctgca atgctaccat atcgttcttc gcttttataa tgttggtaca
    73741 tcaaatagat ttggtattgt ttttgattca acaaaactgc tagacaggca ttctcgtctt
    73801 gacgagattc gcttcggtac gcgctccaaa aatgactgcg taaattccag ccattcgtcc
    73861 agctttcgat tttaggtttg ctcatgccgt acatatcagg caaaagtgca gctgtctgga
    73921 gctgaatctc tttccatttt tgccagtgtt ctttatattc tttcttgatt tgttcaatct
    73981 tatctgttgg atggctctct ttgagctgtt tgaatgcgta gattggtcga tcaaacaact
    74041 gataatctga ttttccaagt tccattgttt cgcctctttc cgttatcttt tgtattctac
    74101 ctgaattttg cgatatggac aaatagaggt tgtgaaaaag ctgttctgca tcaagtcata
    74161 agaacagcca aacaaaaaac tgcgatgagc atcaccgcag ttttattgaa cttaaggttg
    74221 attgatcaca tctaaaagcg ttgaaaataa aatggcacta gaagcagctc ctgggtcgat
    74281 atgtccgatt gcacgctctc ctagatagga agctcgtcct tttttagcta ccaagacttt
    74341 ggtgtgttct tttgcctggt caatgatttc ttttgttaaa ttccctgcct tcaatgcctc
    74401 tgtgactggc agccaaacat ctaacatcgt tttatcgtca gcttctgcct ttcctcggct
    74461 ttgaataccc gctgttccct gctcgatcac ttcgcctaat ttttcttgag aatcaataga
    74521 atcgacctct tttgtcgctt tcatcatgtt catgaatgca gatccgtata acggaccaga
    74581 agccccaccg actttcgata tcatcgccat agagaatact ttgaatgtat cgctgattgt
    74641 ttcaggatga tcggcttgaa aagcttcttc ataagctgaa acccctcttc ccatgttatt
    74701 tccatggtct ccatcgccaa tcggggtatc tagatcactg agatattgtt tgttctgatt
    74761 gattgcctct gcaaattgcg tgagccattc ttggatttct tgaacggtta gtttcatatt
    74821 atcacccttt tatcttgaga ttttttacca tgaaatcgtc tgtacattgc tttctaaaga
    74881 atctttccat tgtgtctccg tcaagtcgat cagtgttaga gaaagacctt gcatatctag
    74941 tgaggtcata aaattaccga cttttcggaa aactacattg acgcctcttt cttccaacag
    75001 agctaaaaca tcattcatga acacgaactg ttccatcaaa ggcgtcccgc ccattccgtt
    75061 gactagtaca cctgcttctt ttggtaaatt cgagtaatca tctaaaatct tagtaacaag
    75121 ttcctttgcc agtactttag aaggctgcat cttttctcga cgatagcctg gctcaccgtg
    75181 gataccaaca ccatactcga tctcatcttc tcctagttcg aatcctggtt tccctacttc
    75241 tggtacagtt gctgctcgaa gcgctagacc gatcgtctta gttgcctgta cgaccttttc
    75301 tcccagttct ttcaactcag ctagggaagc accgtttctt gctgcatcgc ccacgatttt
    75361 gtgtacaagg acagtacctg caactccgcg ttttccagca gtatacgtac tattttcaac
    75421 ggcgatatca tcatctacga tcacactttc tacttcaata tcgtccatag cagctaattc
    75481 ctttgccata tcgaagttca atgcatcccc tgtataattc ttaacgatca gcaaaactcc
    75541 ttttccacta tcggcttcct taatagccgt ctgaatctga tctggagttg gagaagtaaa
    75601 cacatcacca agcaccgctg cacttaacat cccttcccct acaaacccgg catgggatgg
    75661 ttcatgtccg cttcctccac cagaaaccaa accaacttgt tccgctttct gattcttggc
    75721 tacaacacgg gaagattcta cacgatgaac aagttctgga tagcttttta ctaatccttc
    75781 cagcatttct tcgacaatat tgcttggatc attcatgatt tttttcaatt tattcccatc
    75841 ctttctttcc ggcattttgt gcttctgtta agatttgctg agtatcatct gttataccgg
    75901 cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc atccaccaga tgataacgtt
    75961 gctgctgctc ttcttccaac atgtcttttg ccagttccga gttcaatacc gcgctaccga
    76021 tatccgcaaa aacaaaataa ctagctgcat cgcttgattc attgactgct tctacgattt
    76081 tcatcgggtc tgatccgatc tcgccattta ttccgccgag agaaaaaatt tgcacctctt
    76141 cggatttttg catctgctca atcatttctt taataccatc tgttactttt tgactatgag
    76201 aaataagcaa aatacttttt ttcacaagaa tcttcctttc ttttacatag ttgctaacga
    76261 ttggtttcgc tttcattaat gaacaattca agtatataat aaatttctaa ttttgggaaa
    76321 atatgtgtag tgcttcttga agtttgttat actttaatta aaagagagga gtgatcgaat
    76381 gattacaaca acaacgaaca gtgtagaggg aaaagccgta gtagcttata aagggatcgt
    76441 ttttggtgag gtcattactg ggatccatgc atttaaagac ttggaggcgg ggttgcgaaa
    76501 tatctttggc ggccgctcaa aaagctatga aaacgaacta atgggtgcaa gagaagaagc
    76561 acttgccgaa atggccgaac gtgcaaaagc attaggagct gatgcgatca ttggcgtaaa
    76621 aatggactat gaagtattag gcagcgacaa tggcatgctg atggtcacat gtagtggaac
    76681 agctgtctcc acgagataag taggggacat ctgttttaat aactaaagag agatgggaca
    76741 tgtagtggca ctcaagaatt agatttttca gtctaacttc gaggtactct accttcccat
    76801 cctctctttt ttatttcttt ttgcggatga ttccgtaagc tgctgcgact aacacacccg
    76861 cccctgctaa ggaataactg acagaagctg ttttttcatt tgtttttggc aaagtctgac
    76921 ttgttgattt ggatggctct gtcgtttgct gaactgtttc tgtcgtgctt ccagaggtca
    76981 ctgatttttc tcctgccgaa tgcaaaatat cttttattcg ttggaaacta gctgctaagt
    77041 cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc ttcgctgatc tttccgtgga
    77101 ttgcttgact ggccgctgtg atatccgctt gagtttgact tagctttcca gaagctgttt
    77161 gagcaacctg atttaattgc tgattgaatt cttttgcttg ttggatcttc ccgtcaagat
    77221 ctgtttggac agcttccgct tttgctttcg cttcggcaag ctttttctca agttgtgcac
    77281 ggtcatttcc cactttattt ttaagatcag ctaaatcttg atcgattgct gcttggtctt
    77341 ctttcaattt gttttttgca gcttctaatg cttcttcgag ttcttttctt ttattttcca
    77401 agtcttgcaa atgatcgatt tggtcagaag aggtagaact atttattgat gtatcttctg
    77461 aagatgaaat cgtttctcct gaattatctg tcggtacatt ggaagtcatt tctgtttccg
    77521 aggcaccgtt ttcagcacca gaagagctta tggttgttga aggttctgtt tgagcaggtt
    77581 cagaagtttc cgcaaaagct gtctgtccca ataagaaaat actggaaaca gcaattggcc
    77641 agattaacgg cttcattctt tttttcatca cttttcccat ccttactact ttttaactaa
    77701 tttcttatta taagcaaaaa tgtttggaag aaccataaac acaacaggct gtttgtctaa
    77761 aaaacggtgc atcttttatt tttttgtacg ttccgggctt ttccatgtcc cgcttccata
    77821 acctaaaatc gttttgactg ctggaatgaa agtcgtcacg atcgttatcg cgttcgctat
    77881 ccagaacaaa agcatatata gtggtgcgaa aagaaggtat ttcaattttc ttcgtcgatc
    77941 atctacaatc agcgaagcta gcaactggaa aaatcctgcg accatttcaa agcagatgaa
    78001 cacaaaactg aatgtaaaag taatataaat ctgttcgtag tttccttgat atacataatg
    78061 cccgattaat ccagcaaaaa gaactacgga aatacaaaag aatattgacc agacaatact
    78121 caatgtttga tcaatgaaga tgatcgttct tccaatatgt ttaaaaggat ggagcatcac
    78181 ttttttgaaa ttcgtcagcc agacttctgt tccacctttt gcccaacgtt ttctgtgtcg
    78241 atatagcatc ttcaacgtca caggtacttc catgaaaaag ataatctcag gagcaaaaac
    78301 agataaccag tcatttaact gatgatccca agcaatactg atgtcttctg tcgctcgatc
    78361 ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga tacatcgtat ttgctccact
    78421 gtacgcataa agaccaccga aaacagctga ctgtgttcgt ttaatgatgc ccacgatact
    78481 tgagaattca accgtttgag attttgcaat caatttggta cgattttgga catccatatt
    78541 tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg aaataattca catacctaat
    78601 cagtgcatct ggctccggta ccgtgtctgc atcattgctt aaaatcaatt tcccttttgc
    78661 aaaggcaagt ccgatattaa acgcgtgggc ttttccttta tttttttcga tccgaaccac
    78721 acgaagatta gcgtattttt tcatcagtct agctaatatt tctggtgttt ggtccgttga
    78781 accatcgtct gtcactaata cctcatagtt gtgataattc aatttcgtca tcaaatactc
    78841 gattgtatat tcgatcacaa tttcttcatt atgggctggc accatgatcg tgatgaacgg
    78901 ttcgacactc aagggaacat ctacccattc tttttgctta tgtttgtata aaaacacata
    78961 acaccagaca ccaataaacc atacaaatcc gcctaaaatc ggataagaaa cgagaaaaaa
    79021 atacataata gacaaacaag tatctaagaa taaattacct gtatgggtga ttgagatcat
    79081 ttcagttcca ccccattttc tttatacaaa tctgcaatga atgtcgtatc tagatttttc
    79141 tcttcggaga cagaataaaa acggaccgtt tctctctctt cttttgttcc aaaacgttcc
    79201 gtatacactt cttcaagtaa ttcttgtctt tttttcaatt tttcatcttc atattcaaaa
    79261 gatttattta cttttttgta tagatttcga ttgttccacc tagtcaatac aaatgaggta
    79321 atcaggaaac tgaaaaaaat caatacaaaa aagccgatta agaaatacag cattttcttt
    79381 tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt ctggtctcgt taatgggaaa
    79441 aagacccata taaaaggaag aacaacgatc atccatccta aaagagccac taacgtttgc
    79501 caaatcttta accagtaatt accttttgaa aaatacttat ctttcaccaa aattgatgca
    79561 acttcttctc ttgttacttt tttcatatcc tcacctcacg tttttttgtt acgacgtagg
    79621 agtttaccat acatttcatt tcttttcaca taaagtgttc ctaaattttt cctaagaaaa
    79681 agaacaaaac atttctcatc agcaaatgaa aaatattttg ttctttttat cagttgttct
    79741 ttttattttt tatctcttaa ttatgttgct ctttccaata cgacaatgct tttcattcct
    79801 ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct catctccatc gttttcaaat
    79861 ggatataaga ttacatgatc aaattccagt ccttttgcta gactcaaagg aatgatttgg
    79921 cagttttcta atccatatga atttttttta atcgattctt gtagcgtttg ggtttcctct
    79981 tctgtttggg tgataactgc cgctttttct ccttttagag aaaccaaaat ctcagctagt
    80041 attttcaaca attcttcaga ggatcgaaaa gagcggtatt cgggttcttt tccttgcggt
    80101 cggatcgata cgatgtcgat cttctcttga tccacagcat aggttttgaa aagctctgtg
    80161 attgctccgc tagaacggta actatttcta agatcatacc gatggatcgg cagatggtag
    80221 tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag aattaaagat tgcctgattc
    80281 tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt cgatcaatag acctaattgt
    80341 gccagggtat aatcttgtac ttcatcaatc aatagatagc gaaatgctgg tacatttacc
    80401 ttttcaacaa gtaaatgacg aatagctaat ataatgactg cttcatcaac agtacgtgtg
    80461 ggttgccaag cataagctga gccatatcgt ttttcataaa ttctttcaaa caactgatgc
    80521 tcatccaccc atgccatttc ttcgatttgt ctggttattt ttcgatattt tttttgtaaa
    80581 agtttccttg cataagctac aatactttgt tccgaatcat cagaaatcaa tttaccgaat
    80641 aattccaact gcctatcttc tgtcaacgag agtacctgat tctgcacagc aggacttttt
    80701 gcttgtttca aaagatgatt cttccattcg ctcatcagtg cctttttcgt tgcttgaagc
    80761 ctatcgatca atttaggatg agcaggaact ttctcatata ttttttcgat cgttttttta
    80821 gaaaggactg tttttccatt tttagtaagt ccacggataa aattcggatg atctaaaaac
    80881 aaaggatctg attgttttag attatcgata aactttttgc tacgcaaacg ttctgtctgc
    80941 tccgatacat tttctcccgt gatacgtttg aaataagcct cttcaccttc tatttcctct
    81001 gctgaaagct gtgaaaccag ctgtatgatg gttagattca gtggatttct ttcacctaat
    81061 gatggtagga cattcgaaat atagtcgata aaccgatgat taggtgatag aatcagcaag
    81121 tcatcggatg tcatcgtatc tcgatataaa tagagcagat acgcaatacg ctgcatgata
    81181 gcagaggttt taccacttcc tgctacgcca tttaccaaga tattttgact tttcacatcc
    81241 cgaatgattg tgttttgttc cgattggatc gtagcagtga tagccttcat ttcattggtc
    81301 tcatcttggc ctaagacttc taataatacg tcatcttgaa tagctacaga cgtatcaaaa
    81361 aacttgacca aacgatctct ttccaaaaga aattgtcgtc tttctttaat aaccgtatcg
    81421 attttatttt gacgaaccgt ataagaagat ggaccaatca tgttattgta aaaaagttcg
    81481 gcaattggtg aacgccaatc ataaacaaga ttttcatctg catcatctgt gaaatttgct
    81541 gtaccgatat aaaaggattc ttttggctca ttatccagaa agtctacaac gacttttcca
    81601 aagtagggac ttttcaataa acgttcgatc tttgctagct gattttctgc gtttttctgt
    81661 ttgatattca tctgatcgat ttctctattt ttcatttcaa tcatcgaaaa agtatccagg
    81721 ttatctaagt aactgtcaaa attcaatcga agctcgccag ccatttcttt caacgactgg
    81781 tttccttcta tttttgccgt atccatcgat tcccgcagtt cgtttttttt atcaattagc
    81841 ttttgataca cgttcttcag atatgcttct tctaactgtt tttgtgtgtt gaccatacac
    81901 attctcctct ttttttaaag tggtggaacc gattagttta cctcttttaa gagtcgaatg
    81961 caagcttttt aacgagtatc atttgttagg atatatcagt tcttcaggtc ggacagtttg
    82021 atataaagaa aatgcaccat caaggttttt tacgtggtag ccagactgct tcaagattct
    82081 ttccgctaga tagctacgta atccgctatg acagctgacg atatattctt gttggctgtc
    82141 taattcattt aatcggtccc gaagttcatt caaagggata tgtttggctt ccttgaattt
    82201 gcccttttcc aattcttctg ctgttcgcac atctaataaa atttttcctt ttgctagttc
    82261 tgtcggcaat tcatgccatt gtacggtttc actaagccct tctgcaatgt tcattgctgc
    82321 atatcctagc atgtttactg gatctttggc tgagccaaat ggcggtgcat acgtcaattc
    82381 taattccggt aaatcgaaga tcgtcaaatg ccctttgatt gctgttgcta aaatatcgat
    82441 ccgtttatcc acaccttttg ccccaacacc ttgtgcacca taaatctcgc cagtctctgg
    82501 atggaagatc aacttcagta aaatatctgt tgcttctgga taatagcttg catgatcttt
    82561 tcctgataca tgaatgactt caaaagacag ttcgttttct ttggctgttc tttcacttaa
    82621 accggtggat gcggcagtca ttccaaacgt tcgtataatt gctgttccaa taccgccttg
    82681 atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt ctaccttgac gattggctgg
    82741 tgaagcaaga gaaataagtg catcttttcc agtgatttgc tgttttacaa cgatagcatc
    82801 ccccaccgca taaatatcag gaatattcgt ttgataacgt tcgtctacta ggataccccc
    82861 gcgtatgcct aattcgattc cagcggtcac tgctagctgg ttatcaggac gaacaccaat
    82921 ggataaaata actaaatcag cagggatggt acgcccatca tccagtcgca atcgtttccc
    82981 gtggtcttca aatccagcga cagcatgaga agtaatcact tctactcctt gatgagacaa
    83041 ttctttttct ataaaagctg ccatttcttc atccagtgtt ggaagaatat gtggtgcttg
    83101 ttcgatcacc atgacttcta aacctcttct ttttaggttt tctgccattt ccaatccgat
    83161 gaatcctgca ccaacgatca cggcacgctt tggctgtttt tctaatgcat gcatcacttt
    83221 atcgatatct ggtacatttc taatagaaaa aacattgtcg gcttctgtta tccctggaat
    83281 cgatggaaca actggttttg caccaggaga taaaaccaat gcatcgtact gttcaatgtg
    83341 tttttgaccc tcatgtacga cttctaccgt tttgttttcg ggaaagatgg ctgtcacttc
    83401 atgatttgga cgcacatcta aacaaaaacg agcggataat gattctggcg tttggacaag
    83461 aagattttcc cgttcactga tttctcccga aagataataa ggcaagccgc aatttgcaaa
    83521 agaaacatac ggtccttttt caaaaacaac gatttctgca tcttccatta atcggcgaag
    83581 ccgtgtagca gcagacattc cacctgctac acctccgaca ataacgattt tcatcttatt
    83641 tccctcctac tgttcgatca aaccattgat tcatgccacc acgtacattc actacatcat
    83701 aaccagattt ctttaattct ttagctgctt gtttactcct catccctgat tgacaaatca
    83761 catagacggt cttttctttg tctccatcat atctgtttat actttgtaat ggaacatttt
    83821 tagctccttt tatatgcccg ccccggtatt ctgcaggtgt tctaacatca agtaacgctg
    83881 gattttccaa aagcttttcc tttaattctg ggactgttat cgattttatt gattgaaata
    83941 gcaaattcat tttctgcacc tcatttacat ttttataaat acctcatggg gtatattaaa
    84001 caggagcttt attaatatgt caaccagaat gactcttcat ttttttcact actttttata
    84061 tttgcataaa ataccgataa ggggtattat ctaattataa aaatagagga gaatgtgtta
    84121 tggagattga taataaaaat atcctgaacc gtttgaaacg tacagaggga caaattcgtg
    84181 gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga tgtcatcact caattaagcg
    84241 ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt tgcagataat ctgaaaaatt
    84301 gctttgagaa acctgatagt aatccagaag aacaagcggc taaactagaa caagcgatca
    84361 agatgattat taaaaagtaa aggaatgatt tcaatgaaag tcgttttcca tattgatgaa
    84421 ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc tgataaaagc ttctccagaa
    84481 acgacgattg tcgtatctgt aaatggcatc gcgattaccg gttatctaga ctctgccaat
    84541 gcagaattct tagatttgca aggagtcgtt tttcacgctt gcgccaatgc tatgcgtgct
    84601 aatcatattt cagaaagttc tttacctgaa caagtaatcg ttgtaccagc aggtgtcctt
    84661 gatttagttg aactgcaatc ccaaggttat gcttatatca agccttagtc agtagaggtt
    84721 gcgacagaag tgatcagctt caagaaattt cggcttattt ttcgaagaag ctacttctgg
    84781 aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat caagtaataa gaacagccaa
    84841 acaaaaatag cttctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    84901 tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt gacctaagta ttagagaaaa
    84961 aaacgggaaa atagctcctc atattttgcc gttttttgag ctaataccga aggtcagctg
    85021 attgaacacc gtttaaggaa gagattgtga aaaagtcgct tagtttcgag tattaagaaa
    85081 aattttgtga aaatggattt ctacattttt gcataaattt ggcttaatac cgagaaactg
    85141 gcttttgaat accgtttaag gaagaggttg taaaatcagc ggtttgacct gagtattaga
    85201 gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt ttgagctaat accgaaggtc
    85261 agctgattga acaccgttta ccaagaggtt gtgagcctac catttaactc cgaatcacaa
    85321 gaaataattt caaaaacaac ttctcctgtt tttggttcgg ttggcttatt atcgagaagt
    85381 agctactggg agtctattta tcaaaaaaag gaatgcgaca taggtcacac ccctttttta
    85441 ttttctttga aacatctttc ggtactcgac agcatacaag acaaaagatt gtaaaagacc
    85501 gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt ggtaattctg ggaaaagcag
    85561 caaaaggatc atgccaacat agatgacagc agtcgctact ttgccatacc acttcgctcc
    85621 acttaatttg atatttttag atagcccgat taatccagct acaaacataa agccttcttt
    85681 tatcaagaat agaccaaaca acagccaaag ccacggtcga taccatgcca tagacaaaat
    85741 cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc accttcccta agtctgttac
    85801 ctgattatat ctccgagcaa tgaccccgtc tagataatca gacaaacctg agacaacaat
    85861 gaccgctcct gctatataaa atgactgtct cagcacaaaa taaagataaa tcggcagcaa
    85921 aaaaatacgg aatatcgata aaagattcgg aatcgtttgt atttctttac gccagtctgt
    85981 gcttaatttc attttatcgc cctacctcct cagcactctt ttctttttat tttagcttac
    86041 acggggaata aaaccaatga tttcactgtt tttatttttc tttacagaca ctgatattat
    86101 gagctaattt gagataatca cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt
    86161 ttgcgcattt cttttactag tactttatga gctttttttg atcctcgctc taggtcacgc
    86221 tgccgtttac gtgttttata atcgataaac agactgtcta aatcgtaacc tgtcgggtac
    86281 agatcttctg ctgaacgcac cagacgaaca cgctgacgag gcacgtggat tatttttttg
    86341 tctaaatata catcaatcgt atcttcacct atatcttcat aaactaacgc ttctttttgg
    86401 tattcattga caaatatccg atcacctttt gcaaagagct gacgttcttt tttattctcc
    86461 ttcctatttg taactgctgt cttaggaaga aattcttttt tctgtgtccg ataggctttt
    86521 tccgccaagt aacgctctgc ttgatggatc aatttcatcg acatatccat tttatgagca
    86581 atccacaaag cttggctttc tcctgtttct tccaacccgc aactgatatt taggacgaag
    86641 tgcttcccga tcgaaagcca tagcagccgg cacaaaatct tcatgatctc cggcgaattt
    86701 cttgatttct ccataatggg tcgtggcaat gatcaatgcc ccttgctcat acatagattc
    86761 catgattgcg atcgctagtg cagccccttc atttggctct gttccactgc caatctcatc
    86821 taaaagtacc aacgtattcc tttttatctt tttcaaaata gcagcgatgt tttgcatatg
    86881 accagaaaat gtactcaacg cattttctaa attttggtgg tcaccgacat cgacgaaaat
    86941 ctcatcaaag acagcaagct ctgtcccttc ttttgccgga acttgcatac cgaacatcgc
    87001 catcaaagtc aataaaccga ctgtcttcaa cacgactgtt ttgcctccgg catttgctcc
    87061 ggtgataatc aagcctcgat agtctttacc gatttcaaca tccaatggta ccgcatgctc
    87121 aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt tccgatttat tgattctagg
    87181 tgtgcttcca ttgatttctc ggctaaattt tgcccgagca aagatgatat ctaacaccgt
    87241 gatcgtttcg atcaataaat cgatcacttc ttcgttttct gcgatagcac cagtcaatgc
    87301 cgccaggact tgatattctt cactgatttc ttcagctttt aacaactgat agtgttcatt
    87361 caatttagaa atcacagttg gttcaataaa aaccgtcgtt cttttatttg attcatcgat
    87421 aatcgttcca tctattttat ttttatagct ggctttgata ggaatcgtat agtattcgcc
    87481 tttttgaaca atcattgctt cttggatcat ttctttgttt ttcggatgac gcaagaattt
    87541 cagtaatttt gactggattt ctttttcgat tagttgtaat tgtttgcgta ctttacgcaa
    87601 attacgtgac gcgtcgtcac tgactttatt attttttatt ttctggtcaa tcaactcttc
    87661 tacatttatc aaatcaggaa gatgtttgct ataagcaaac aacaagggtg tctggtattg
    87721 attcttatca aaaaactttg tgatcatccg actgcttcta agaaaatccg catattcgat
    87781 caaatcggtt ggctgcagaa tcaacccttt tttgacttgt tctgtcagtg catcgatcct
    87841 cggcaacccc ataaatggaa catgctgact gctttctaag atcaagcgtg cttctcttgt
    87901 ttcttcttga cgatccaaga cagtttgtag gtttgtcgag accgtcatat cgctgattcg
    87961 ttttttgcta tagtttccaa ttgctcttgc ttggatttcc tttttgatcc gatcaaattg
    88021 taattgacta atcatttcgt tgtccatatt ctctcctttt cttgaagaga tacctgtccc
    88081 aaaataaata agggcctaga gataaataaa aggatagaac acagcttaaa aaagtcatgt
    88141 cctatcctta ttagaataca aagatattgt tttcctgaca ggtaccgttt cataagcaca
    88201 ccaaataaac tttgtttatt ggctattcga cttactgatt agataccagc tacactcaca
    88261 aaacataccc cattcttcta ggtcttattt attttccact taactatagc aagacacatt
    88321 ttggttgtca aactttagta tgttttatag atttacacca ttaatgaata gacacctacc
    88381 gcgataattg ctccgatcat tggacctgcg attggtaccc atgaatatgc ccaatctgaa
    88441 gttcctttat tagctattgg caataccgca tgtgctaatc gaggaccaaa gtcccgagca
    88501 gggttgattg catatcctgt tggccctcct aatgaaagac cgatggctaa gatcaatcca
    88561 cctacagcga atacgttcgt tccaggtgca aattcgtttt gtccaaaagc tagcaaccca
    88621 agaactaata caaatgtacc aattaattct gtgatcacat ttgctggata attccgtata
    88681 gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat ctttcgttgc attccaatgt
    88741 ggcaaataag aaagccaaac aagaatggcc cctgcaaaag ctcctaaaac ttgcgcaatg
    88801 atgaaaggaa cgacaagatt ccaactaatt gcccctgtca tcgccatagc tagagagact
    88861 gcaggattca aatgagctgg gcccatgaat ccagaaatat agacagccat tgttacagcg
    88921 agtccccaac caaatgcgat gacaatccag ccagaagcct gtgctttact tttgtttaaa
    88981 ttcacagccg cacacacacc atctccaagc aaaactagga tcagtgtccc aataaactcc
    89041 cccatgattt gagtcatatc actgttcatt tatttctccc cctttagttc ccgcagatct
    89101 gattctgcaa tcaatgcttc taaacgttta gtctgttgcg ccttctcctc ttctgtccat
    89161 tcaaaatatc cagcaattgc atcgatcaca ggttgcttga tctcatcaag ctgatctcgt
    89221 tcaaacaaca gatgatttgt acgacgaatg agataatcgc ctggggccaa aaccatttct
    89281 tcttctaaac cataacgtaa ccgagctgac tcagcgagac tcaaaccagg ataaggtgcc
    89341 atttcttttg ctaattcaaa gatacgtcgg gcattagtcc cgtaaaaatc agcgatataa
    89401 gtagcatctt cttcctctaa accggctgct actccttctt ttgctaattc tgtcactgtt
    89461 tcttctaatt tagttggatc gaagtttcca cctgaaatct gatatttttt agagtcgatt
    89521 tctttcgtct ctattccgta ttcttctttt aataattggc gtatcaatcg caaagctcct
    89581 tctgccatct tacggtaatc ggtgattttc ccaccagata aagtcagcaa accatctggt
    89641 tctctttcta aagaactacc tctggaaatc gtagaaggtg ctttttcatc acggctgttt
    89701 tccaaatggt tcaacacgtc ttctacttca gcacgagaaa ctttgttttc cttatattca
    89761 cttactgtat cgacaacttt attgaagctc ttgtctgaaa tcgatccatt atctccacca
    89821 ttgtaatcag aaccagaatt accaatcaac agtggacgaa gtcctgccca gcttgcttcg
    89881 atatcattca atgtgatatt tgcttctgga tagcgatgat tgatcacatc caatagataa
    89941 tctacatctt cttgtgtgac tttcggatcc gtaaaatctc cttggtaatc cgtatctgtc
    90001 gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa ccatccgccc atcttgcttt
    90061 cctgtatcga agtatgtggg ttgcggtaca ggcagttttt tcgcatctac aactaaatgg
    90121 atccctttag ttggacgcat tttaggagag actggacgcg taaaattcaa gtttcgtact
    90181 ttatctaccc aaggaccact cgtattgatc actaattttg ctttgatttc gatcacttca
    90241 tctgtcagca ggtcacgagc tttgacgcca acgatttgat cgccatcata taaaaagcca
    90301 accgctttca ttttacttac tagataagcc ccatcttcgg cagccttttt gatattatca
    90361 atcactaaac gggcatcatt gttgcggaaa tctagataga cacccgcgcc ttttagccct
    90421 tcttttttca aaaacggttc tctttccaat acttcttctg gtgtcagcgt ataattctca
    90481 tatttggttc ctgtcacatt tgccagtttg tcataaaggt ccattgctac tttgaccgag
    90541 aacatattga aggttgttgc tccttcgccc tcatagattg gcaaaagcat tggatctggt
    90601 tttgggatgt gtggggcaat actttgtacg actgcacgtt caccaactgt gtcagctact
    90661 acttccacat caaatgtctt cagataacga ataccgccat gaacaagttt ggtcgagcga
    90721 gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc ctgttttgat tcctgatgct
    90781 gctgactgga ctgccacacc ggcaccagtg attccgccac cgatgatcaa cagatccaat
    90841 tcttcttgct gcattttttg aatgctatct tgtcttgtct tgtttgaaaa catatggatt
    90901 tcctccttat tcaaatgctt gggtagcttt tactgctttt ttccaaccgt tgtaaagttt
    90961 ttctcggcgc tcttcctcca tttgaacttc aaatagcttg ccagcttcat aaaattcgcg
    91021 gatctcgtct gtatctttcc aataaccgac agctaatcct gctaaaaatg ctgcccctag
    91081 agccgttgtt tccaagtttt ctgctcgttg gatcggtaca ttcaagatat ctgtttggaa
    91141 ttgcatcaga tattcattgt ttgctgcacc gccatctact ttcaatacag gaatcttgat
    91201 tccagtgtca tcttgcatcg tatcgatgat atctctcact tgataagcaa tcgactgtaa
    91261 agtagcttta atgatatctt cttttgtcgt accacgtgtc aagccaaaca tagcccctcg
    91321 tgctgcctga tcccaataag gagcacctag cccaacgaat gcaggaacaa cataaacctc
    91381 atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg ctagcttttt gcaacatttg
    91441 aagtccatct ctcagccatt gaatcgaaga accagccaca aaaatacttc cttctaaagc
    91501 atagtaaacc tttccattta tgccgtaacc aattgtcgtc aataaattat ttttggataa
    91561 ttgcggtttt tcaccggtat tcatgacaat aaatgaacct gtaccataag tattcttgac
    91621 catgcctggt tcgaatgcca tttgtccgaa taatgcggct tgctggtctc ctgccatccc
    91681 tgaaattggt gcttcgcttc cataaaaatg ataaccttgc gtataaccat aaacttctga
    91741 gttagaagtg acttttggta acatcactcg aggaatattt agtaaatcta aaatctcctg
    91801 atcccaatct agctgatgga tattgaacaa catcgtccga ctggcattcg agtaatcagt
    91861 aacatgtgct tgtccatcgg tcagtttcca tactaaccaa gaatcgatcg ttccaaacat
    91921 caattcgcct ttttcagctc gttcttgcgc gccttctaca tgatcaagaa tccagcgtat
    91981 cttcgttgct gagaaatagg catcgatcac taaccctgtt ttttcgtgga aaaattcttg
    92041 atagccttca ttttttaagt tatctgcaat tccagctgac tgtctggatt gccaaacaac
    92101 tgcatgatag attggacgtc ctgtatcttt ttcccatatt actgttgttt cacgctggtt
    92161 cgttatgcca atcccagcaa tctgatttgg tttgatccct gattcgatga acgctccggc
    92221 aataactgac tgtacagaat tccagatttc atttgcatca tgttccaccc aactttcttg
    92281 cgggaaatat tgcgtgaact ctttttgaga actgccgatg tgtcttcctt tcttgtcaaa
    92341 gataattgct cgtgagctag ttgtaccttg gtcaatcgcc atgatatacg ttgattctgt
    92401 cattgtgatt ccctccatta tatgcaaacg ctttctttaa ctactcttat catactactg
    92461 ttttaaaaaa agaaactgtc aaaattttat tctttttttc tttttttgac agttttttat
    92521 tatgaagcta ttttttaatc aattcttttc ttttttcagc gtctctattc ttcttttcaa
    92581 tcgaataatg tcgtaaggtg acgcaaattc tgacagataa tattcacact gttttttgtt
    92641 tctctggcaa aatgtgatca ccaaatcata ttctttacct tcttcataac ttccaatctc
    92701 aataccgccg attcccctca gttctctgat cagatattgt tgaaaagcaa tacgataaat
    92761 cggcagactt tctaaatcga ttccaatcac tactggtttt gcaatataaa aatcgatcat
    92821 caaaagaaca ttggcatact ggatcatcag gtaactaagc tcactatctt cagctctttt
    92881 cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc agtaactgtg ttaaggaatg
    92941 cccgagcatt ttctgctgct gttccaaaag ccgatcacgc tcatagactt cgatctttcc
    93001 aacaaagaaa aaccattcat tgttgatctg ggaaatttgg tagcctactg ccttttcttc
    93061 tttgattttc aaacgacgag gacgataatg ccataacatc gtttcccgaa tgtaagtatc
    93121 taaaacagcg gttggtaact ttttgctccg tgtcaaatca tactgataaa atatttcttc
    93181 gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc attgtttcgt atacattcag
    93241 ttctgctgct gttcgactaa gatgcatcac gatgattgga tcgattgctt gataaagccg
    93301 gtccgactga tagagtaatt tcttttcttt caaagttgcg tacgtacttt tttcattcaa
    93361 tagtcttttt ttcgtaatcc cgagccagca agcaatctgt gcttctgctg atgccgtaaa
    93421 cgttgtttct aatacacggt ccaatcctag aatgaacggt cttttttctg gtgtattttg
    93481 caaaaaaagc ggtcgctgat cttccgggat atattggaat agttcataat aaaaatatcg
    93541 tatctgtagc tcttctccgt ataattgacc gtttttgatt tgtaatccaa attcctctaa
    93601 gagctggttc aattctttga tctttcgaaa aacagaagac tcactgatat taaacgctgt
    93661 cgctaatcga acgatggata cttgctgatg ttctaataaa tagacgagca tcttaaactt
    93721 caagctttcc tgcaataaaa acaatagaat tttttccaaa cttacatctt cgtccaacac
    93781 aagcagcgcc tgatactcat ttcgctgtac ttctactttt ttttccatca ttcggccaat
    93841 caattcaata tcttccaaat attgatcaaa cgaggctttt gacagttgga catgctctct
    93901 caaaccattg atagaggctg ttcccccttg cagaacaagg tttcgcaaaa tcaggatctg
    93961 taactgctct tttctttcta ataatgcttc aatcttcata ggcagtattc gccacctttg
    94021 accacaccat atctggcaca cggtattctg gctggaaaaa tagctcatgt tcttctaact
    94081 gttcaaacgt ccatccttct tctactgcaa ttgtcagttg gtcaagaagg tggaacatct
    94141 cttttttgct ttggagctgt gctccaagaa tctttttttc agaatcaaat agaaatttga
    94201 gtttaacttt gctttgattt attgctgtaa gcttgtagtc tacgtatcct gtttcaattt
    94261 tttggggata aaaaatgctc tcttcttctg tcaaacctac gcttcctaag aaatagccaa
    94321 accactgatt gccgactgga cgataggttc cctgatcctt tgtctgtgag ccactaattg
    94381 tcttgctgac atttcgtgct gtacggacag cgttatttac tagagaaaca tacatttttt
    94441 catttgttgg acgaaaagtt acttggatcg catcgccaat cgcataaacg tctttttgag
    94501 acgtttgcaa gtagtcatct acccagatcg ttccatcatc attcaatgtt aattgttctt
    94561 cccacatctg attgtcggga cgagaatgat tagccagcaa aacaagatca tagttctcct
    94621 ttcttttttc cgtctctaca atagcggttt cattttcggt caaagacaca acttgttctt
    94681 ctaaaaaaag tgaaagccct tgatttttca tttcctttac taacggttct gtcatttcag
    94741 gatctagata acgaaaaaga attgactttc ggctttcgta aagatggatt tgtttttttc
    94801 ttgatgccaa cccttcagct atttcgattc ctgcttgtcc tgcaccaata attgcaattt
    94861 tctttgcatt catgatcttt tgctgaagag ccgttacgtt ttgtatcttt ttaactgggt
    94921 gaataagcgt tgattctgca gaaatatttc gaaacttttg atttgaacca gtcgctagaa
    94981 tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac gacttttttt tctttcaatc
    95041 ctattactgt cttttctgtg taaacatgga ttcgatagcg gtcagtcagt tcttctttcg
    95101 tgatccaaga aagttgtttt tcactggtaa cctttccttt taatatcaaa ttgaacccgc
    95161 tgggaatcca tcctaacttc gattgctttt caatcaaata tatttcactt gttggcatca
    95221 attggcgaag ataaatagcc gcatgaatcc ctgcaaacga cccgccgaca attacaatgt
    95281 tcatctgcat tcctctttct tataaggtgt tctactgcta acttctcaat cagaaaacac
    95341 agtattcata actcgctttc aaccttatga tacatagcga tgcagcagaa tcaaccctct
    95401 ccatcatacc gtgttctagg tctgcttctc aataatgtac tagtggacct aattgaagag
    95461 tatcatttct gaagagatgt ttcaaaaaat gtactcttat aaaagaaaaa aactcgcctt
    95521 ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa aagacgagtt ttagcgtgtg
    95581 taatgtaata gttagaatag acacatcatt tatttttaat tctttaatgc ttcaacaact
    95641 gcttcgccta attgatgagc agattgagga ttttgccctg taagaagccg cccatcaatt
    95701 tgaaccactt ctgaaaaagc tgctccagac tgatagtttg ctcctttttc tttcaaagca
    95761 tcttctgcta agaatggtac ttcattcgtt gtaccatttg cttcctcttc ttcattactg
    95821 aacccagtca ctgtacgtcc agctaaaata gattttccag actcatctgt aactggcaac
    95881 aatcctacaa caccgtgaca aacagctgag accagccctt catttcgata gatttgtgaa
    95941 gcgactttcc caagatctat tgcttctgga aaatcccaca ttgttccgtg gccaccagca
    96001 taataaaccg ctgcataatc atttgcatcc acatcatcag gacgtaaggt atttcccaag
    96061 gcccgatttc tgaaatcttc atcttcataa taggcccaat cgatttcatc catattcaaa
    96121 acactttgcg gttctaaagg tacatatccg cctttaggac tgacaaaatc tgcatcgatc
    96181 ccatttttta ctaatacatc ataaaaatga gtcagttcac ttaaccacaa acctgtagga
    96241 cgctcgatct tttcgtactt ttctacattt gttaatacga tcaatacttt cattttccca
    96301 tcctctttct ataaaaattg cgttattgat aacgtgcttt caagaaatta tataatgggc
    96361 ttggcaaata aagctcttta tatgcttttt caaaaggttt cgttacaaat tcatcctcac
    96421 ggttttccaa taatttctct gcccagtcag gatcgacgat catttgctgt ccaattgctg
    96481 caagttctgc atatttcaat acctgctcta catcttcgcg attgcggatc ccaccgacac
    96541 caactaaagg gatctttcct tgaatcgtgt tatggaccac ttcaagaatc gatttatcgt
    96601 tgtatttatc agaacgagct attcgatcat acatatttaa tgaaacatgc agataatcta
    96661 gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc ttcaaaacgg atacctggtg
    96721 tttcatattc ttctggtgag aaacgatacc ctacgatgaa tggtttttta gcatattgtt
    96781 caaccgctga gaaaaccgtt gttaataatt cttcaataaa acgataacga ttttccaagc
    96841 taccgcccca ttgatcctct cgtctattcg aatgcggaga gaaaaattgt tggatcaaat
    96901 acgtatttgc tccatgcaac tcgattccat caaatcctga ttcaatcgct ctacgtgtgg
    96961 ctttgccaaa tgcttggatt gtttcaacga tttcttcttc actcattgct cgtggtgttt
    97021 ccgcatcagg acgttttgct gcaactgcac ttgcagatac aggctgttct ccagataaag
    97081 ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat aatcgctttt gcgccttctc
    97141 cttggattgc tttcgctagc tcacttaatc gaggaatcat ttcatcatga gcgatgctga
    97201 gttcacctgg ccagcctttt ccgatatctt ggacatttgc agcaccagta ataacagcac
    97261 caagaccttt agaacgttgt ttataataag tgatctcatc ttcggtgatc actccattga
    97321 aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg gttcttcaat tgaactcctg
    97381 aaggtaaagt aattgattga tcaaattgtg acattgtcat tcctcccata attcgctctg
    97441 attaacctat cgagaatcat ctatcattaa accactcgat tagttgtaca tgcaactaat
    97501 ttgcttaaaa aattttattt tttttgtaac ttatctgttg catcattcat tgtctgggct
    97561 aattctttac ttgcttcatt tcccaaaact gctttgtatg actgatgaag ttcttcccag
    97621 ctaccatcga cttccttagc aaattggcgt ccttgttctg taagataaac taatgacaag
    97681 cgaccttcgc taactgtttt gacgtatcct ttatattcta atttttcaac aaaacgagca
    97741 atcgttgatg gggcggaata gagcatttgt gcaatatctt tttgtggtat tccatctttt
    97801 tcttttaata tcaataaaat aaaaccataa gttggtgcca gacctgtcct tctaaacgct
    97861 tcatcagcta atttattcaa catgcgatct agctttttca cgctaaagaa aagacattca
    97921 tctaacgctt tttcaattgg cttttccatt tcatcccctc cattggcgta catatcatag
    97981 tatgattttt attttttgtc aaaaaacacg gaataatgtc cgcctcctct gtctagattt
    98041 gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt tttcgacaaa actccttctg
    98101 gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt ttgagtatga agaaaaattt
    98161 tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaaa aactggcttt
    98221 tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct gcatcaagta ataagaacag
    98281 ccaaacaaaa atagttcctc atattttgtc gttttttgag ctaatgccga tggtcagctg
    98341 attgtacacc gtttattgag aggtcgtgag cctgccgttg aactccgagt cacaaggagc
    98401 aatttcaaaa aacagcttct catgttttat gactatggtg acttgtgatc gaggagttgg
    98461 caggagaata ctgtttattc gtaaagaagg ggctgtgaca agacttttgt cacagtccct
    98521 tatttccttt tattatagga tttcgccatt tgattggatg acttgtctgt accaatcaaa
    98581 tgattttttc tttaaccgtt tctttgtgcc attcccttcg tcatcttgat caacatagat
    98641 atatccataa cgttttgaca tttgtgaagt agaagcgctg acgatatcaa tgcagcccca
    98701 cgacgtataa ccaaataaat ctactccatc tttgatagct tccttcatct gctcaatatg
    98761 ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt ccatcttctt ctactttgtc
    98821 tatagctccg agtccgtttt cagctatgaa cagtggtttt tgatacctat catataattg
    98881 attcaaagca attctcaaac ccttaggatc aatctgccag ccccattcac ttgatggaag
    98941 atacggattt ttaattcctc cagttattaa gtttccttgg accccttccc aattttctgg
    99001 atgagcactg actgcactag acatgtagta gctaaaagaa acaaaatcaa cagggtaggc
    99061 tgctaagatt tcttcatcac ctacttctat ttttaagttg acaccttttt cttcaaacat
    99121 tcgttgcgta tacgctggat aatatcctct tgcttgaaca tcactaaaga acaaagaagt
    99181 tctttgctgc tcaattgttt tttgaatatc atccggatca caagaatacg gaacaagaag
    99241 ctgatagctg atcatacaac cgacttgtga acctttgata atttcatgac aagcttttgt
    99301 agctaaagca cttgctataa actgatggtg tgcaccttgg tatttcgctt gttccaaatg
    99361 gggatggttt tcttcaatta ctcctacgct aacaaaaggg tgatgtttta cacagttgat
    99421 ctcattgaaa gtcatccaat attttacttt atttttatag cgtttgaata ctgtttcagc
    99481 gaaacgaacg taaaactgaa taagctttcg attaggccat ccaccatatt ctgtaaccaa
    99541 atataacggc atttcataat gggaaagaga aacgactggc tcaatcccat attttagaca
    99601 ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca ttgggtgttt cttcatcacc
    99661 atgaggaaaa attcttgtcc atgcgataga catccgatag catttaaaac ccatttcagc
    99721 aaataaacgg atgtcttctt taaagtgatg atagaaatca tttccgtgtc ttttaggata
    99781 ataatacttg ctgttgggat cctgcgccgc ttttacttgc tgatgcgtca tcatatgcca
    99841 ttgatctgtc catttttctt taggtaaatc gctgtcgaat gtatacacat cagacacaga
    99901 taatcctttt tcatctacat cccatgcacc ttcacattga ttggccgctg tcgctccgcc
    99961 ccataaaaat ccttctggaa attttttcat atcgatcccc ctaaacttgc tagttttctt
   100021 gtttgattat ttttcttatg actagtaatg atatttttga atctactact atttgtaaca
   100081 ataaccattg tggtcggatc atatccctct ttgattaatt cttgcaaatc tacttccatc
   100141 aataaatcgc ctttacgtac tttttttcca tcagatactg cactttgaaa gtatttgcca
   100201 tttaaattca ctgtatcaat accgatatgg atcagtatct ctgctccttc ttcactgact
   100261 accccaattg catgtttcgt tggaaaaact gttgaaactt ccccgtttac agaactgtag
   100321 atttttccgt ccgttggaat gatcgcaaac ccttctccta aagatccact agcaaatgtt
   100381 ggatcgttta cttcagataa atcaattatt tcacctgctg caggagtact taacactata
   100441 tcctttgagg attcttcttc gatttcatcc ttgtagccga aaagaaaagt tagcatacag
   100501 gaactgatca tcgctaagaa ataactagca actagccaaa caaatccttc accaatataa
   100561 atcgggaaag acaaaatact tcttgtagca actgccaaag caccggctcc tgcggcacca
   100621 gctactccac caaatatagc tccacatata caagcaatgt aaaatggtcg tttatattta
   100681 agagtcacac cataaacagc aggttcagtg attccaaata atgctgtgat aaatgcagat
   100741 aaagataaac tttttacttt cttatttttc gttttcaaaa ataccccaag tactgctcct
   100801 gcttgcgcag ctacggcagt catacatgct ggacctagcg tatcacgacc gtagaaagaa
   100861 agattgttgg tcattactgg tacaagtccc caatgtaacc caaaaatcac taacacttgc
   100921 caaaatccgc cgataacagc accagataaa acagggctaa agttataaac agctaaatat
   100981 ccagctgcaa tcagctgact tgcataatct gcaattggac ctacaactaa taaaataatt
   101041 gggaccatca cgatgataca aatgaatggt gtcaataaat ttcgaatact ttcgtgcaag
   101101 cttttgcgaa tcttaggctc aatataagaa agaacccata cagctaaaat tgctggaata
   101161 accgtacttg tataccttgc tagaatcacg ggcatcccta gaaatcgcaa ggttatgcta
   101221 tcactatatg cactgacaat cgtaggataa acgagagctg ctgcaataac catagcgatg
   101281 aaacgatctg tattgaactt ttttgcagca gtatatgcca aaaatatagg caagaaatag
   101341 aaaaatgcat cagctgctgc attcaaaatg atataagttc ctgattcagt agttaataaa
   101401 ttcaaactcg tacataaggc gagtactcct ttgatcaaac cacttcctgc caagacgcct
   101461 aaagtcgggg caaaaacagc ggcgatcatg tctataaaag agtttaaaat gccttttttc
   101521 tcagttcctc tctttccact agatgcatca tcttctagtt tcatttgttt ggataatacg
   101581 tcatacactt catttactgc atttcctact acgacttgaa acattcctcc agcttcgact
   101641 acagtgatca caccttctag ttgctctacc ttctccttat ctgccttctt attatctttt
   101701 agggtaaagc gtaaccttgt tgcacaatgg taaacattgc tgacattttc ttcgcctccg
   101761 acaaatcgga caatatcttt cgctaattgt gaatagtcca tttttacttc ccccttgtta
   101821 attaatgccg tagcaatcct tcttacaaga taagtatagt tcttagaaaa cggtttctcc
   101881 attcaattag taccccttct aatagcgaac gaaactgtac aatagtgtgt atggtttact
   101941 tagccgagca aatcactcac tgcttgaaag aaaacaagta tatcgtttct cttatagtca
   102001 aaggagacaa aaaaaacacg cttgatcttc ctttgctgat gtataagtgg tatctcaagc
   102061 tgatactctg tgatttttgt ttctacttgg cttttgataa gacagcacaa caaagtctcg
   102121 gcatttaacg gaagcataac taggtcagaa ctaggagaga ttagttgctg aatggaatct
   102181 atctttttat ggatttttat ttgattttct tctttgtagt aatgatcaag cagttttgtt
   102241 ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa tctgataaat ctcccaagat
   102301 tcaaactcat ctagaacact aggtagataa atcaaatcat cccactcaaa aataagctct
   102361 tgctcagtag tcaaaattgc atcatattcc gttattttgt ttgattgatt tttgaaaaga
   102421 tacactggtt ctatccggca ttctttgatc cacatgttca tactttgttg aatctgtttt
   102481 tttagtgtat tcaaaatact aattggctgg ttactaacaa tcagtatctc cccttgtttt
   102541 cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat aagcgctttc agctaaattg
   102601 ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta aataaatttc taaaggataa
   102661 cgggagatga tcttttgtac aaaatgattc atggtattat gtccggcatc catcctcgtt
   102721 ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat ggaacaataa cgaaacagga
   102781 agatctaaaa aacgaataat cgtttgctca aatgctctta gattcttctc tatttctttg
   102841 tttttttcat cattaatcat caaataattt aactctaata gtcgattttc aattgcttgt
   102901 ttttcttgat tggaaaaaga atagggtagt tctgatgata acttttttgt gagggtctct
   102961 accatcggaa taaataatgt atacctgttt gttttttcta aaagttcttc tcttcgaaga
   103021 atggtcaaaa cgagataccg agaaaatcgt atgaagtcct ctcctgaaac aatataatta
   103081 tatgatataa gaacctcttt taatgtcttt tggacagtcg gcatgatatt ggaaaaaatt
   103141 gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg tttctgttcc aacaagagat
   103201 aagaaaattc tttttgaatt ttcttcagca atgattttta gtccttttgt cgacgaaact
   103261 tccagttcta atttaggatt gcgctcgatc caacgcaaaa tcgtttttat ttgtaatgat
   103321 acggctgttt ttgaaagaaa gaaaatgtct gctatctttt gcatactgac ataccctttt
   103381 tgaaacaata agtacgtcac aatagttgaa gctcggaaat ccatgctgga atagatttta
   103441 tgtttcgtca cctcttgtat gatttgctgt ttagtttcag attcaattga ttctaaaaaa
   103501 taacctgaac gtttatttga ctggataatg attttcttct cgtagatagc aagcatattt
   103561 ttgatttcag tttggatcgt tttcttgttg gtacctaaag cttcagctaa ttcttcgctt
   103621 gttatccaat gattcttatt tcttaatact acaagaattt ggagttgtcg tttatttaag
   103681 tccatttgct tacacctcct ctttgttata aacatacagg aaaaacctct caaatgacaa
   103741 tagtcacttg cagaggttta aaaatcattt gaagaaagat ctatattcaa ggtttttacc
   103801 attagactgg ataatttgtt tataatattc aaaactatct tttttcaatc tcctaaagct
   103861 tccgctccca tcattttgtc tatccacata aataaagcca tatcgcttcc tcatttcccc
   103921 tgttcctgcc gatacgatat cgattggtcc ccaccataag taaccaatga tttggcaacc
   103981 atcttttatt gcctcagcaa tttcctgaag atgtattttt agatactcac aacgttttgg
   104041 atcgtggatt cgattcaatt catctggtgc ttcatctagt ccaatgccat tttcaactat
   104101 gaataatggt aattcataac ggtctgtcaa ttcattcatg acataacgaa accctagagg
   104161 atcaattggc caactccaag gttcgggaga tttttccttc aaaaagggat tatcgaaacc
   104221 atttgctccg ccagtatcta cgcgcatttc tgcttctttt ttatagacag cgctacgata
   104281 ataactaaat gcaatataat ctgccgtgtt agcttgcaag cactgttcgt ctccatctaa
   104341 cattactggt gcacagtctt cttccttcca tgtcctgtaa atataagctg gataatgccc
   104401 tcggcacatg acatcggaga aaaaccatga attgcgttta aattgcagtg aaccaaatac
   104461 attctctgga tcacagtcat atgcataagt cgctaatgca gaaaagctac acataatacc
   104521 catcttagca tttggatcga ttttctttaa taattgagta gcacgggcgt tcgctagaaa
   104581 catatgatga caggcttgat aaatcacttt ttttgttagt gtagcaatgg gttcaagttt
   104641 atctctagca gggaatgtaa ccaatccagc agctgcataa ggcatacgaa aagcattatt
   104701 gatctcatta aaggtcatcc aatatttcac tttcccttgg tagcgttcaa agacggtctg
   104761 cgcataaaat tcaaaaaagt cgattaattt tcgattttgc caccccccat actctcctac
   104821 caatgctaag ggggtttcat aatgactaag agtaattact ggctcgatat catatcgatt
   104881 taatgtattg attagtctgt cataaaatac gagaccagcc tcatttggct tctgttcgtc
   104941 tcctcttgga aatattcgtg cccatgagat gcttgttcga aaagctttta aacctaactc
   105001 cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa tcaatcgctt catgagaaag
   105061 ataatggagt gattcgtcta aagcaatctc ccaaatcttt ttttcttcat tccactgaat
   105121 actaggatgc tggttcatga tcccgataag cgtatctgtc acattaggta atttgccgtc
   105181 ttccaaccat gcaccttcac attgattagc agctactgct ccgccccata aaaaattttc
   105241 tggaaatatt tcctgatcca taactatctc tcctcgctat caacaatttc ttacttgaag
   105301 tatagctcga ttcatcaaaa caagatattc taactatttc ggcagaagag aagaaaaaaa
   105361 ttgaatgcta ctcttcattt ttaatattta tatataacaa atacataaga cgatccccga
   105421 aagaaaacac tcgttaacca tctaagcaaa cgatttattt tacttccaac acaaatgctt
   105481 cataaggtcg taaatcaact ttgccgtctg gtaactgttt cagaggataa tttgacacaa
   105541 taacccttaa agcttcacgt ggaatagtga acgtctgctt ttcactggac atattacaaa
   105601 taacgagcca ttgctggtct tcatatatgc gagaataggc aaagacatgc tcaggcgtct
   105661 tttccactaa ttcatagtct ccccagacga tgatgtcatg ttttctacgc aaatcgatca
   105721 atttcttgta catataaaaa acagaatctt gatctgctaa cgcttcttct gcattgattt
   105781 ctttataatt tggattgact cgaagccatg gaattccagt agtaaaccca gcatgttcac
   105841 gggcgttcca ttgcataggc gtcctagcat tgtctcttcc tttcgccttg attgattcca
   105901 agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt atacatattg attgtctcaa
   105961 tatcttttgc ttccgcgata tcatcgattg ggcaattcgt catgcctaac tcttctcctt
   106021 gatagatata aggggtgcct ttcatcatat ggagcaagat agcaaacatc ttggcacttt
   106081 cttttcgata cacatcgtca tttccccaac gagaaacaat tcgcggaaga tcgtgattat
   106141 tccaaaataa actattccat ccttcatcac ctaaagatgt ttgccatttg gataatactt
   106201 ttttcagttc agagatactc aagggtttca aatcccattt gtctttgcct tcttgctgat
   106261 ctaatcctat gtgttcgaat tggaagacca tggataattc atttctttta ggattggaat
   106321 aaagtttagc gatttcggta gttgctcccc atgtttcgcc aacagtcatc agatttttgt
   106381 caccaaaagt tgcttgattc atttcctgaa gatactcatg cagtttaggt ccattacttg
   106441 tgatttcttt atccggttct ttgccaatca tatcgatcac gtccatacga aagcccccaa
   106501 tacctttatc gatccagaaa ttcatcattt gataaatctc ttggcgaacc tgtttatttt
   106561 tccaattcaa atcaggttga cgcttgctat acaagtgcag aaaatattgc cccgtttttt
   106621 catccaactg ccaagcagaa ccactgaata ttgagcgcaa tctattaggc tcttttccat
   106681 tgaccggatc acgccaaata taatagtccc gataaggatt ttctttattt ttacgggcct
   106741 caatgaacca cggatgttca tcagaagtat gattcaccac cagatccatg atgatacgga
   106801 tatttctttt ctttgcttct tcaatcagtt cttccatttc ttccatcgta ccgaattctg
   106861 ccatgattgc ttgataatta ctgatatcat acccattgtc atcattcgga gactcgtata
   106921 ctggagaaag ccaaattgca tcaataccta aatacgccag ataatccaac ctggaaataa
   106981 tcccccgcaa atctccaata ccatcttgat ttgaatcttg aaaacttctt ggatagattt
   107041 gataaacaac tgaatggtgc caccattttt gttccatgat tttcctccta taaatggatg
   107101 tttctatttt ttgcttaatc atttatttta gtctctctca ctaaaaattg taacgctttc
   107161 aaatagctat tgcaccttgt tatcggtaac agaataaaac aaaaaaacag ctgaggaaat
   107221 tccctcaact gttttttgaa aggattgatt tatgcaggga agaatgctga tttcacaact
   107281 tctttataaa cggtgttcac tcagctgtcc cttggtatta gctcaaaaaa cggcaaaata
   107341 taagaaacta ttttttcgtt tttttctcta atactcaggc cagaacgctg atttcacaac
   107401 ctctttattt cacattcaat atccacatga atcccacaaa tacaaagaac agaccataca
   107461 tgattggaga tacttctttt cctcttttag ctgctaacat ggtaattgga tagaagataa
   107521 aacctaatgc aatcccatct gaaatactat aagtcaaagg catgccgatc aagatcaaga
   107581 atgaagggat cgcaatctct aagtttcccc attcgatttg tcgcaaagat tgtgccatca
   107641 atactcctac cataatcaat gctggtgcag ttacttgtga agtaacaact gatagtaatg
   107701 gagaaaagaa caagcctaac aagaagaaaa tccctgtagt aatagctgtg atacctgaac
   107761 gtcctccaac ggcgatacca gctgatgatt ctacaaatgc accaacagga gaagtaccaa
   107821 ataaggaacc cgcaagcata gctgttgaat cagaagctaa agctttccct acacgaggca
   107881 tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc aactaaagtt cctgctgtat
   107941 caaagaaagt cacaagcaag aatgtcagca ctacgaccca catttgaaga gaattgatgt
   108001 cccctacatg ttttaatgca actaaaaatg ttggtttcaa acttggtgct gcggcaacaa
   108061 tttcagaagg cattggaatc aaacctgtga ccagacctaa aatcgttgta gctgccatcc
   108121 cgatgaagat gccacctggt acacgccgaa caagcaagat agcagttacg actaatccaa
   108181 aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa tcctactaat gtagattcat
   108241 tcgaaacgat gatcccacct tcgctcagac caaggaaagc gataaataga ccgataccgc
   108301 cagaaatcgc atatttcaaa tctgctggga tcgcatcaat gatcatttcc cgcagcttga
   108361 agattgtgat caaaataaaa atcaaagaag caacgaatac gccggctaat gctgtttccc
   108421 atggaatccc cattcctaaa caaacagaat aagcaaaaaa agcattgatc ccaagagcag
   108481 gtgccgttcc aattggataa cgggctaata cccccattaa aacacagccg atcgcactgg
   108541 ctaatgcagt cgcagtaaac actgctcctt catccattcc agaagcacct aatacagttg
   108601 gatttacaaa taagatataa gccattgaga taaatgttgt aaatcctgcc agtacctcac
   108661 gttttaccgt ggtgttcaat ttttctattt cgaagtagga aagtattttt tccttcatct
   108721 tccatccccc taaatccgaa cattataaat aaagtccgtt aaaatttcga tttattaata
   108781 aaacaacgtg ttttatcata tactaaaaca aaacataagt aaacaaaaaa ttcggatttt
   108841 aagcaaaatc cgaacgttcc atctgattaa atccctttat ctcaaagttc atgaacattc
   108901 actttggatt acaagaattt ctttattgtg ttaataaagt ttcttcattc tagtctcaaa
   108961 aatcaatagt gtgatttgat tttaccattt cgtttctgtt cttctttgat gaattctttt
   109021 gtatcgacag gattcagatt atttagtccg ccaaaacctg tatcgaccat ttttccccag
   109081 ccttttgtct tcttttttcc tgatttgaga ttctttgctc gaagcttctc actatgtttt
   109141 gctcgccgat acttcaaata accttctaat cttcccatta gatccactgt ttttcctcct
   109201 ttttattcaa tcagacgact cagcttttcc tataacaaaa taattgcctt cagggtcaga
   109261 aaaattgaaa gtcaagatgc cattgttatc tgtgatttct cctggttctt ccatgttctc
   109321 gtagatagtt tcaaattcat ccgtaaaaaa tactaaagaa ggcggtggtc ctaatacctc
   109381 tggtgaaaac ttctgcacaa atacttttgg gaatacgcct aattctaatt cttttgagat
   109441 gctgagtaca atactcttaa attcttctgg caattcgatt gtttcagaaa tttctgcacc
   109501 aattttttct acaaagaatc ggcaaatcat ctctacgtca ttgacataaa gcatcacacg
   109561 tgttttttgt atcatcattt gacccttctt tctatttgat tttctttcct gtctataaac
   109621 tgattccatt atatcacgaa agcgcaaaca ccttgtgttt gatactattc aagaaaaata
   109681 aagcaatagt ttctaggatt gctttcctcc acaaactttt tttacaaaaa aaaaacggca
   109741 atgaatgccg ctcttcttat ttcgattctt ttataattct gcttctgatt catctttggt
   109801 tagtgtgtgt ctaacactag ttacgccaaa tgtttgatcg attttttcct tcaacgcttc
   109861 gattgcctgt gcgccacgtg ccgcttgttc agcattgatg gattcaatga ccagcaccgc
   109921 tgcttgtgtt tcttccgtca gcatcgttct ttgggcttca cgccagttga gacagcgaca
   109981 gatcgctcct tgttcatcat agtagatgat ttcttccgga agtgccggag catcggaatc
   110041 tgcacctaaa ggcaggaatg attcgccacc ttgggctttt cctaaatgca gatttcctgc
   110101 gatcttttcg atatcttctc cgccgcaagg tacgccaaaa gacaaggaaa tactattata
   110161 gatatctaca agcggattga ttgggaaaaa ttcccgttct tgatttgcac gtttcagtaa
   110221 agcttcaata gaagaacgtg cacctttttt agtcttgaat gtggtaaatg cctgacgcca
   110281 ctgagcaatg acttcgtttt ggctgaatgt ttcttctgta aggtaatttc ttgcttcttt
   110341 tgctgcctca ttcaataatt gatgatacgt ttcttcactt tcttttgtta cgtggttatc
   110401 gataccattt acggataaga tttcgatggt tgcttcagga aatacttccc agaaattggt
   110461 atccactatt acttttttca tctgttcaca cctcttgatt agaataattg cttgatgaat
   110521 agatagtctg tttggggatc atctcccata taaaaaacat gttcgctgta tttgataaat
   110581 ccttttttac gataaaaagc gagcgcaaca tcgttgtgtt cccaaacacc gagccaaatg
   110641 gctgtttttt gcttttcttt tgctttttca atagctgtat ctagtaaaaa tgtaccgata
   110701 ccttgatgtt taaatttttt tcgtacatag attcgctcga tttctaaaaa atgatcagcc
   110761 atttcctcag attgactcgt tcctgtattt agtttcaggt aacccgcagg ctcttgatcg
   110821 atactcacta ggaaaaaatc ggtttcttta tcttttagct cttctgtaag tttttctagt
   110881 gcataagctt gtgtcaagta atcagctaaa tgctccggtg aattatcttt tccaaatgta
   110941 tctgtaaatg tttcgatact aatttgttgt agtaagtcta catctgaagg cgtcattttt
   111001 cgaatttcgt atttattcac ggcttcttcc tttcaataga tccttttatt gccttttttg
   111061 acaaaattcc aatcttcgct aacattcttg catactttat ttaaaagata ttccaattgt
   111121 tttgcttctt cgtcagaaag accatttaat gcgactgcat tggagtagtt gttttctcga
   111181 atgatgaaag gatagatttc tgctcctttt ttagtcggat agagtttctt gattttttta
   111241 ttttctttat cttccaaacg ttcgatcatc ccattggatt ctaatttctt gattgctcgg
   111301 gcagtcgttg tacgatctac tttgatcatt tctgctagtt tttcttggat gattccagga
   111361 ttttcgcaaa tacgtactaa ataaagatac tgtcctcttg ttaaatcgac ttctttgaat
   111421 tcgatattgc taatagaatc tagcgctcgg gcaatcattc cgatgtctcg aaggatttca
   111481 gccatttcat cccaccttta ttttgtttgt aagactagtt cacctctttt tgttgcaaat
   111541 gcaactattt tcaataaaaa aatcaagttc ttttttaata actagtaaag tatattcttt
   111601 tcttctttta cgaaaaagct caatcacaat agatctggtt tttgttttct aaaataagta
   111661 actgtttctt cgttgttcgc ttttctgata gctttggcaa ctcttctata ctaaaaaatc
   111721 ccatgttgga agtttcatta ttctcaataa acttggcctc ttcaccgtgg atcgcacaag
   111781 caaaaacaag tttataatac tgaaaaagct gtggaatatc tttttgttta ttcgtatcaa
   111841 aaactgctcg caactgagta gtttctactg ttaatcctgt ttcttcatag acttctttac
   111901 gcacattttc ttcaggagac aatcctattt cagcaaatcc accaggaagt gaccattctt
   111961 tcgtccgttt atcttcgatt agtaggacct tgccatcttt tttgatatag gcacgaacat
   112021 ctacttttgg tgttggatgt ccttctccaa tcgttgtgag cgcttttatt tcttcgaaag
   112081 tttcttttcc tatttcactg atcagttcca atgaaatcgt ccggatctcc tcaaaacgct
   112141 ctttatcaaa tacatctttt ccataatata gaccagcttg agaaagtgcg agtagtcttt
   112201 tgtattgagc caacttcttg ttcattctct tcccttcttt tttagcaaat gtatgactat
   112261 gtaatcaagt atagaagact ggtttaatta cggcaaataa atataatgaa atctgcaata
   112321 aataaaagaa tgttaaaaaa ctcgagaaca aaagacattc gacttctctg gggctgtgaa
   112381 taaatggcgt tcaaaagctt gcatctgcgg taataagccc caccaaacga aaaatatgac
   112441 gagctattct tgtctggctg ttcttattaa ttgaggcagg acagcttttt cacaacctcc
   112501 ccactaaacg gtgttctcct accaactcct cggtcacaag tcacaatatt catcaaacat
   112561 gagaagctgt ttttttgaaa ttgctccttg tgactcggag ctgaacgaca ggctcacgac
   112621 ctctcggtaa acagcgttca aaagccaatt tttcgatatt aagccatttc tttgttactt
   112681 attgtcattg atttcttttt ctaatttatc caatagtgga gtgtaatcca cttgagtata
   112741 gtctgttaca tcaaattcat atagtgttcc taactggaat ttgtcatatt ttcggtcggc
   112801 gatgatcttt ttgaatgctt ctttcgtaat atgattggtc cctttcgttc gatcttccaa
   112861 tacgtcacca tagtgataat gatccatgat atagctcaaa tgacgatctg attcacgatc
   112921 acgtttgtac cgacgttgcc ataacacttc aaagtctgct gttaggcgaa tcgtgatcac
   112981 ctgatatccg tatctatctg ctaattcttg gagtcgtgct ttttgcttat cactaaaagg
   113041 atactcggac acaatcactt tctttcctgc ttccatatac aaatcaagtg ctttgtaata
   113101 gaaaggataa actttcgtcc tttcttgttc cgcccgttct tccaaattgt taaacccgat
   113161 ggattctgca tagtattcct tgatttcatc gagcgtgatg gcatacatat ctggaaatcg
   113221 ttcccgtagt ttattcataa gatatgtttt tcctgtacca ggactaccag ccagaagaat
   113281 gaaatacttt ttcatctcat ttatttccta agttaattgg actttccaag ttcataaaca
   113341 gtccaatctt atgcttgaca atttctttta ctgcttcatt tgctggaatc acattatggc
   113401 gaagagactt gttgatttct gtttcactaa aggtttcttt agctactttc gtccagttga
   113461 ctaaaagctc cgttccaaca ttgaatttgc ggatcccgtt attgatcaat tcaggataat
   113521 cttcttcttt cacacctgtt cctccatgga taactagggg gacatcaact actgcatcga
   113581 tttctttcag caagggaatg tttacttctg tttttgattt gaattgtcca tgattcgtac
   113641 cgatagcaat agctaatgca tccacgcctg tagcttctac aaatttcact gcgtcttccg
   113701 gtcttgtata taccttgtca ttttcagcga catggattcc ttcttctgta ccgccgattg
   113761 ttccaagttc tccttctacg gaaacaccgt gcgcatgggc atattctact actgcttttg
   113821 tcttcaggat attttctttg aaaggcagat gggacccatc atacatgaca gaagtatatc
   113881 cgctgtcgat tgcttctttg atatcatcaa agtctcgggc gtggtctaag tgcaagactg
   113941 catcaaccat ttcttcttct gcaatagctg tcattacatt gactaacaca tgcatgccga
   114001 tatatttagc ggtatcgaca cttgtttgaa taatgatcgg cgaacccatt tcttttgctg
   114061 cgcctagcat agcaggaagc atttctaggt tatgcgtatt aaaagctcca actgtaaaat
   114121 tcaactcttc tgctgttttt gttacttctt tcaatgttgt atacatcttt tattgccacc
   114181 ttatcttttt atttgtttcc tttgatcgtt acttgttttg caatttgtcg catctcatcc
   114241 attttgccca tgtagtaatc gatcccttga tcatcaccag cacgagcagc atctgcacgc
   114301 ttctggttat agaggctcat cagtttttta tacccatctc cgatcgtctg tttattttcc
   114361 aaactctttt ctaaatattg gatggcttga tcgttcacat acaattcaga aaatttaagt
   114421 ccaacttgtt cataaagttt cgctaattta tgcttatctt gggtagtgga aatctcttgt
   114481 ttcaattgtg taatctctga ttgaatcttt tcggtcgctt ctggcgatag ggctttttct
   114541 tcttttgcca gaactttttt cggttctttt tcttttttct taaaaaatcc aaacatcatt
   114601 ttccctactt tcaattaaaa tgctttcaaa actgggctta agatccaggc atataagagt
   114661 gctgcggcta ctgtatctac atcggttgct gtagcattgt tgaatcccat cgaattaaag
   114721 atcgttacta gcaaagcggg caataatgta ataaagaaac catgagcaat accgccaatg
   114781 atcgctcctc ggcgtccgcc aactgcgttg ccaaatatcc ctgctgtccc tccggcaaag
   114841 aaattggtta gcataccagg taaaatcatc gctaagccga acatgggcaa agtaaacatg
   114901 gcaataatcg taccaatcgt tgtcgtaatg aaacctaaaa tgacagcatt tgggctataa
   114961 ggaaagaaaa ctggacaatc taaggcaggg atcgcatcag gaactaattt catagctatc
   115021 cctcgaaatg ccggcacgat ttcacctaac agtagacgta caccagctag caatacatac
   115081 acacccacta cgaattggat ggcttgtaaa aaggcaaaca tgatatagtt ttgatctcct
   115141 gataattcag aagcgaatgg ttcgcctgca aataaaacag tgatgatata tagcgggacc
   115201 atcacgacca tgacagacaa ataagtatct tgcagaaatt cgaaatgtgt cggcaacttg
   115261 atatcttcga tcgacttttt attttcgcct ttctcaccaa aaagcttagc aacaccggct
   115321 tcaaataagt acccgatcgt acaaaaatga cctaaagcaa tatcatttga tccagtgatt
   115381 ttacgaatga tcggttgcgc tactgccggc atagcaatgg cgaaacaagc tccgataaaa
   115441 ccaccgacca agatcaatac gatccctcgc agaccggcaa aatagccaaa gactgtcgtc
   115501 atcgttgcca tccataaaat tgcttgacca gttaaaaaaa tgtacttcca ttttgtgaaa
   115561 cgagccaaaa taatattgaa tacaaaaatg gctaagaacg tcagtgcaat atctctacct
   115621 aaacccaaat cattcattgc ttgaccattg atagattcaa tagaagggat gatcccttgc
   115681 atatgaaatc cttcagtaaa gattttcccg aaataagtta aacttccaac gatgatgctc
   115741 gaaccggcac tcaacacttg aaatcctagg agtgtcttta acgtgccaga gatgacttgt
   115801 cctgctgatt tcttctgcag tagtagaccc aacatagcaa tcaatgcaat ggtgatcgat
   115861 gcctgagtca aaatattttc gatgataaaa ttgataacgc ccatgttttg atgtgcaccc
   115921 caaaagtta
//
LOCUS       DACDRN010000004       105657 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDRN010000004 DACDRN010000000
VERSION     DACDRN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 105657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 105657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..105657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.004"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..95)
                     /locus_tag="IWJ80_000387"
     CDS             complement(<1..95)
                     /locus_tag="IWJ80_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9460156.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVA"
     gene            complement(107..595)
                     /locus_tag="IWJ80_000388"
     CDS             complement(107..595)
                     /locus_tag="IWJ80_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9460157.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(672..3368)
                     /locus_tag="IWJ80_000389"
     CDS             complement(672..3368)
                     /locus_tag="IWJ80_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9460158.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(3796..4869)
                     /locus_tag="IWJ80_000390"
     CDS             complement(3796..4869)
                     /locus_tag="IWJ80_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9460159.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(4866..5693)
                     /locus_tag="IWJ80_000391"
     CDS             complement(4866..5693)
                     /locus_tag="IWJ80_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9460160.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(5694..6500)
                     /locus_tag="IWJ80_000392"
     CDS             complement(5694..6500)
                     /locus_tag="IWJ80_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9460161.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(6512..7597)
                     /locus_tag="IWJ80_000393"
     CDS             complement(6512..7597)
                     /locus_tag="IWJ80_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9460162.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(7614..8156)
                     /locus_tag="IWJ80_000394"
     CDS             complement(7614..8156)
                     /locus_tag="IWJ80_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9460163.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            8414..9403
                     /locus_tag="IWJ80_000395"
     CDS             8414..9403
                     /locus_tag="IWJ80_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9460164.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            9400..10137
                     /locus_tag="IWJ80_000396"
     CDS             9400..10137
                     /locus_tag="IWJ80_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP9460165.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            10134..10685
                     /gene="coaC"
                     /locus_tag="IWJ80_000397"
     CDS             10134..10685
                     /gene="coaC"
                     /locus_tag="IWJ80_000397"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP9460166.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            10701..11333
                     /locus_tag="IWJ80_000398"
     CDS             10701..11333
                     /locus_tag="IWJ80_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9460167.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            11418..12065
                     /locus_tag="IWJ80_000399"
     CDS             11418..12065
                     /locus_tag="IWJ80_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9460168.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            12070..13053
                     /locus_tag="IWJ80_000400"
     CDS             12070..13053
                     /locus_tag="IWJ80_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP9460169.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(13456..14970)
                     /locus_tag="IWJ80_000401"
     CDS             complement(13456..14970)
                     /locus_tag="IWJ80_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP9460170.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            15108..15914
                     /locus_tag="IWJ80_000402"
     CDS             15108..15914
                     /locus_tag="IWJ80_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460171.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(16089..16658)
                     /locus_tag="IWJ80_000403"
     CDS             complement(16089..16658)
                     /locus_tag="IWJ80_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9460172.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(16741..18801)
                     /locus_tag="IWJ80_000404"
     CDS             complement(16741..18801)
                     /locus_tag="IWJ80_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460173.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            complement(19078..20556)
                     /locus_tag="IWJ80_000405"
     CDS             complement(19078..20556)
                     /locus_tag="IWJ80_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9460174.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(20854..21789)
                     /gene="murB"
                     /locus_tag="IWJ80_000406"
     CDS             complement(20854..21789)
                     /gene="murB"
                     /locus_tag="IWJ80_000406"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP9460175.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(21862..22782)
                     /locus_tag="IWJ80_000407"
     CDS             complement(21862..22782)
                     /locus_tag="IWJ80_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9460176.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            22895..23647
                     /gene="xth"
                     /locus_tag="IWJ80_000408"
     CDS             22895..23647
                     /gene="xth"
                     /locus_tag="IWJ80_000408"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP9460177.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            24511..25044
                     /locus_tag="IWJ80_000409"
     CDS             24511..25044
                     /locus_tag="IWJ80_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP9460178.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(25534..26058)
                     /locus_tag="IWJ80_000410"
     CDS             complement(25534..26058)
                     /locus_tag="IWJ80_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9460179.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(26051..26524)
                     /gene="tsaE"
                     /locus_tag="IWJ80_000411"
     CDS             complement(26051..26524)
                     /gene="tsaE"
                     /locus_tag="IWJ80_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP9460180.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(26647..27627)
                     /gene="pta"
                     /locus_tag="IWJ80_000412"
     CDS             complement(26647..27627)
                     /gene="pta"
                     /locus_tag="IWJ80_000412"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP9460181.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(27780..28460)
                     /locus_tag="IWJ80_000413"
     CDS             complement(27780..28460)
                     /locus_tag="IWJ80_000413"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9460182.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            28586..29455
                     /locus_tag="IWJ80_000414"
     CDS             28586..29455
                     /locus_tag="IWJ80_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9460183.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            29481..29996
                     /locus_tag="IWJ80_000415"
     CDS             29481..29996
                     /locus_tag="IWJ80_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460184.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(30043..30606)
                     /locus_tag="IWJ80_000416"
     CDS             complement(30043..30606)
                     /locus_tag="IWJ80_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9460185.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(30661..30840)
                     /locus_tag="IWJ80_000417"
     CDS             complement(30661..30840)
                     /locus_tag="IWJ80_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460186.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(30917..31426)
                     /locus_tag="IWJ80_000418"
     CDS             complement(30917..31426)
                     /locus_tag="IWJ80_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460187.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(31522..31902)
                     /locus_tag="IWJ80_000419"
     CDS             complement(31522..31902)
                     /locus_tag="IWJ80_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9460188.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            32232..32981
                     /locus_tag="IWJ80_000420"
     CDS             32232..32981
                     /locus_tag="IWJ80_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP9460189.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(33165..34442)
                     /locus_tag="IWJ80_000421"
     CDS             complement(33165..34442)
                     /locus_tag="IWJ80_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460190.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(34521..35165)
                     /gene="pcp"
                     /locus_tag="IWJ80_000422"
     CDS             complement(34521..35165)
                     /gene="pcp"
                     /locus_tag="IWJ80_000422"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP9460191.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(35364..36308)
                     /locus_tag="IWJ80_000423"
     CDS             complement(35364..36308)
                     /locus_tag="IWJ80_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP9460192.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(36310..36996)
                     /locus_tag="IWJ80_000424"
     CDS             complement(36310..36996)
                     /locus_tag="IWJ80_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP9460193.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            37469..37789
                     /locus_tag="IWJ80_000425"
     CDS             37469..37789
                     /locus_tag="IWJ80_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP9460194.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            37950..38855
                     /locus_tag="IWJ80_000426"
     CDS             37950..38855
                     /locus_tag="IWJ80_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9460195.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            38852..39625
                     /locus_tag="IWJ80_000427"
     CDS             38852..39625
                     /locus_tag="IWJ80_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9460196.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(39802..41226)
                     /locus_tag="IWJ80_000428"
     CDS             complement(39802..41226)
                     /locus_tag="IWJ80_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9460197.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            41437..41883
                     /gene="nrdI"
                     /locus_tag="IWJ80_000429"
     CDS             41437..41883
                     /gene="nrdI"
                     /locus_tag="IWJ80_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP9460198.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            complement(41953..43935)
                     /locus_tag="IWJ80_000430"
     CDS             complement(41953..43935)
                     /locus_tag="IWJ80_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9460199.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(43949..44845)
                     /gene="murQ"
                     /locus_tag="IWJ80_000431"
     CDS             complement(43949..44845)
                     /gene="murQ"
                     /locus_tag="IWJ80_000431"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP9460200.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            complement(44848..45909)
                     /locus_tag="IWJ80_000432"
     CDS             complement(44848..45909)
                     /locus_tag="IWJ80_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9460201.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(46121..46648)
                     /locus_tag="IWJ80_000433"
     CDS             complement(46121..46648)
                     /locus_tag="IWJ80_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9460202.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            complement(46715..48217)
                     /gene="lsa"
                     /locus_tag="IWJ80_000434"
     CDS             complement(46715..48217)
                     /gene="lsa"
                     /locus_tag="IWJ80_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9460203.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            complement(48475..49596)
                     /locus_tag="IWJ80_000435"
     CDS             complement(48475..49596)
                     /locus_tag="IWJ80_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9460204.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            49722..50663
                     /locus_tag="IWJ80_000436"
     CDS             49722..50663
                     /locus_tag="IWJ80_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9460205.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(50806..51702)
                     /locus_tag="IWJ80_000437"
     CDS             complement(50806..51702)
                     /locus_tag="IWJ80_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP9460206.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            51984..53324
                     /locus_tag="IWJ80_000438"
     CDS             51984..53324
                     /locus_tag="IWJ80_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9460207.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            53519..53962
                     /locus_tag="IWJ80_000439"
     CDS             53519..53962
                     /locus_tag="IWJ80_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP9460208.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(54224..55642)
                     /gene="nhaC"
                     /locus_tag="IWJ80_000440"
     CDS             complement(54224..55642)
                     /gene="nhaC"
                     /locus_tag="IWJ80_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9460209.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(56046..57269)
                     /locus_tag="IWJ80_000441"
     CDS             complement(56046..57269)
                     /locus_tag="IWJ80_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP9460210.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(57301..58140)
                     /locus_tag="IWJ80_000442"
     CDS             complement(57301..58140)
                     /locus_tag="IWJ80_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP9460211.1"
                     /translation="MKKIIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(58157..58837)
                     /locus_tag="IWJ80_000443"
     CDS             complement(58157..58837)
                     /locus_tag="IWJ80_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9460212.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(58839..59912)
                     /locus_tag="IWJ80_000444"
     CDS             complement(58839..59912)
                     /locus_tag="IWJ80_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9460213.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     gene            complement(60562..61233)
                     /locus_tag="IWJ80_000445"
     CDS             complement(60562..61233)
                     /locus_tag="IWJ80_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9460214.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(61321..63999)
                     /gene="mgtA"
                     /locus_tag="IWJ80_000446"
     CDS             complement(61321..63999)
                     /gene="mgtA"
                     /locus_tag="IWJ80_000446"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP9460215.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASPNGLS
                     EEQIERSRETYGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELITGEKISEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLM
                     PLPGNYWLWFFITVVAYLALVTAVKKIYIRRFQELL"
     gene            64659..65096
                     /gene="ndk"
                     /locus_tag="IWJ80_000447"
     CDS             64659..65096
                     /gene="ndk"
                     /locus_tag="IWJ80_000447"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP9460216.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(65118..65984)
                     /locus_tag="IWJ80_000448"
     CDS             complement(65118..65984)
                     /locus_tag="IWJ80_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9460217.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(66218..68296)
                     /locus_tag="IWJ80_000449"
     CDS             complement(66218..68296)
                     /locus_tag="IWJ80_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9460218.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            68789..70006
                     /locus_tag="IWJ80_000450"
     CDS             68789..70006
                     /locus_tag="IWJ80_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9460219.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(70465..71088)
                     /locus_tag="IWJ80_000451"
     CDS             complement(70465..71088)
                     /locus_tag="IWJ80_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460220.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(71244..72641)
                     /locus_tag="IWJ80_000452"
     CDS             complement(71244..72641)
                     /locus_tag="IWJ80_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9460221.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(72663..73634)
                     /locus_tag="IWJ80_000453"
     CDS             complement(72663..73634)
                     /locus_tag="IWJ80_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9460222.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(73641..75941)
                     /locus_tag="IWJ80_000454"
     CDS             complement(73641..75941)
                     /locus_tag="IWJ80_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 3 glycosyl hydrolase"
                     /protein_id="HAP9460223.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(75943..77268)
                     /locus_tag="IWJ80_000455"
     CDS             complement(75943..77268)
                     /locus_tag="IWJ80_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9460224.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            77499..77924
                     /locus_tag="IWJ80_000456"
     CDS             77499..77924
                     /locus_tag="IWJ80_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460225.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            complement(78422..79123)
                     /locus_tag="IWJ80_000457"
     CDS             complement(78422..79123)
                     /locus_tag="IWJ80_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9460226.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(79139..79579)
                     /locus_tag="IWJ80_000458"
     CDS             complement(79139..79579)
                     /locus_tag="IWJ80_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HAP9460227.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTITSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            complement(79579..80154)
                     /locus_tag="IWJ80_000459"
     CDS             complement(79579..80154)
                     /locus_tag="IWJ80_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP9460228.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(80565..81071)
                     /locus_tag="IWJ80_000460"
     CDS             complement(80565..81071)
                     /locus_tag="IWJ80_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAP9460229.1"
                     /translation="MDYKKRVQQMFLDSGVVLKETELERIEYTDFGLGAIETEGLNLF
                     VYENNERYCGKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKL
                     SCIIPKGQERYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDS
                     FTNPAIYR"
     gene            complement(81082..81561)
                     /locus_tag="IWJ80_000461"
                     /pseudo
     CDS             complement(81082..81561)
                     /locus_tag="IWJ80_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(81733..82434)
                     /locus_tag="IWJ80_000462"
     CDS             complement(81733..82434)
                     /locus_tag="IWJ80_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9460230.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(82542..83984)
                     /locus_tag="IWJ80_000463"
     CDS             complement(82542..83984)
                     /locus_tag="IWJ80_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9460231.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            complement(83998..85863)
                     /locus_tag="IWJ80_000464"
     CDS             complement(83998..85863)
                     /locus_tag="IWJ80_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9460232.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(85984..86835)
                     /locus_tag="IWJ80_000465"
     CDS             complement(85984..86835)
                     /locus_tag="IWJ80_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9460233.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(87145..87858)
                     /locus_tag="IWJ80_000466"
     CDS             complement(87145..87858)
                     /locus_tag="IWJ80_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9460234.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(87926..88252)
                     /locus_tag="IWJ80_000467"
     CDS             complement(87926..88252)
                     /locus_tag="IWJ80_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9460235.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(88375..88602)
                     /locus_tag="IWJ80_000468"
     CDS             complement(88375..88602)
                     /locus_tag="IWJ80_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9460236.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(88924..90177)
                     /locus_tag="IWJ80_000469"
     CDS             complement(88924..90177)
                     /locus_tag="IWJ80_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9460237.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            90238..90978
                     /locus_tag="IWJ80_000470"
     CDS             90238..90978
                     /locus_tag="IWJ80_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9460238.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(91064..91822)
                     /locus_tag="IWJ80_000471"
     CDS             complement(91064..91822)
                     /locus_tag="IWJ80_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9460239.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(91819..92847)
                     /locus_tag="IWJ80_000472"
                     /pseudo
     CDS             complement(91819..92847)
                     /locus_tag="IWJ80_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(92894..96034)
                     /locus_tag="IWJ80_000473"
     CDS             complement(92894..96034)
                     /locus_tag="IWJ80_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460240.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEIT"
     gene            complement(96258..97142)
                     /locus_tag="IWJ80_000474"
     CDS             complement(96258..97142)
                     /locus_tag="IWJ80_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9460241.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(97237..97674)
                     /locus_tag="IWJ80_000475"
     CDS             complement(97237..97674)
                     /locus_tag="IWJ80_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460242.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(97891..98802)
                     /locus_tag="IWJ80_000476"
     CDS             complement(97891..98802)
                     /locus_tag="IWJ80_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9460243.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(99259..99687)
                     /locus_tag="IWJ80_000477"
     CDS             complement(99259..99687)
                     /locus_tag="IWJ80_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460244.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(99715..100353)
                     /locus_tag="IWJ80_000478"
     CDS             complement(99715..100353)
                     /locus_tag="IWJ80_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9460245.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(100357..102393)
                     /locus_tag="IWJ80_000479"
     CDS             complement(100357..102393)
                     /locus_tag="IWJ80_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP9460246.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            102519..103184
                     /locus_tag="IWJ80_000480"
     CDS             102519..103184
                     /locus_tag="IWJ80_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9460247.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(104675..105547)
                     /locus_tag="IWJ80_000481"
     CDS             complement(104675..105547)
                     /locus_tag="IWJ80_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9460248.1"
                     /translation="MIYGAIEAGGTKFICAVSTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQRKSKKYGHITSTPKLAWQNFNFVGTLKQHFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMLSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIASYIVTPELGDSAGI
                     IGGLALARKAIRNK"
BASE COUNT        30916 a        21957 c        18108 g        34676 t
ORIGIN      
        1 gctactacta acccggttaa atttagactc tttgtccaag tcatcgctac ttgtccatga
       61 attaatcgtt catcaattcg cactgcttta atcatttttc ttcctcctaa aaaaaatctt
      121 cctcttcatt tactacatgt acctcatcaa gaatggtcat ctttaattcc tgttggcatt
      181 tatttaccag ttcctgaata tcctcctttg ttaattcctt ggttgccaat actatttcga
      241 gcagaattgg cagattaaag ccagacaaaa tagttacagc aatctctttc tccaaaaagg
      301 ttttggtaat cttctgattg acacttccgc caaataaatc ggtaaaaaca aaaatctgat
      361 cctgctcgtt cacctggttt acaaatacat tcaactcctg ttcaaaatca cactcatcca
      421 cataagcatt aatcaccgta atctcttgaa ccttccccgt cagtaattga atactgctaa
      481 gaaagcctga agctaagtga ccgtgagtcg ctattagaat tttcttcata tggttaattc
      541 ctttctttct gttcactttt atataagcaa ttcctgtgcc aacttttaaa aacattggtt
      601 taatcacgtt aattcacatt aaaaaaagtg tctaacctaa aaaaaacaga ttagacactt
      661 ttttagacac tttatttttg aatatacggt cctaataatt catagagcag cgaaagctca
      721 taagcattga ctttaaaatt atatttcatc tccattgatt gaaaaatact tctagaaaca
      781 taataaaatt catcttcatc tggtttcaaa tcataattcg gttcttcaga tttttctgtc
      841 cgagccaaga ataaacgttc catcatcaat gccgtatgca tatagagatt tagttttaca
      901 cggccattta aagtgaaatg atagtaattt tcatatttag aaatgactgt ttccacttca
      961 ctcatcacga tcgttgggtt cagaaagttc aaacggtccg caattccttc caacgaaaag
     1021 aattttacta gctcctgaat caagcggtta aattctgccg gcttcatttc aggactcaaa
     1081 atatgctcca aattggctgc tccatcaccg tctaagatgt cataaacatt aatattagga
     1141 attgaaaagg aacgtggaag atccgaagtc gtgatgacca attttgtctt gtcaaaataa
     1201 gtttcatcat tttggtcgat taattcacgt agttctctgt agtccttcgt gaaaacttcg
     1261 atggattcat ccgataaatg atgacgaaaa acatctttca atttgtccga aatccccaaa
     1321 cccgacatac aggaaatgat aatgttgcta ctttgagaaa agccttcaaa gtattgaaca
     1381 ttgattgaat acttgtctgc tgcatcctcc gcaatctgtt taaatggcgc attcatcgtc
     1441 atcttcaagc caatatcaag tgcaatggaa gtcgtcaaat tattcagaag aagtaactcc
     1501 ccttttaaat cgttcttgat ctgtgtgtaa atttgattta aagagcccat atctacgatc
     1561 agcactagac tatctgtatt cacttgccgt ttcacgaaat tccttgtcga ttcgacaatc
     1621 tctgacacat tactctctac tggcatatcc aacgcttcaa aaacataatt gccgcataat
     1681 tgattcacta ctgcttgaat gctgctagcc gtactatctc cgtgagcaag caacaggccc
     1741 ttcagctcga tggattcatc tacataatca gataaagtca cgacaataag cagtcttaga
     1801 aagttctcgt ccttttctga gagcggtggt aaatgctcca agaactgatt acataagtaa
     1861 gtcgtccgag gtagatttgc agctaatgta cgatttatcc cggttaagtc tgtcttatag
     1921 cttggggaaa tgacatagga tctgctaata atttctgcaa caggtccact cgtcgtcaat
     1981 ccgtacgttt ttcctaaaac ggatacccag acctttctaa acatctgaat catcaatttt
     2041 ctactcattg ataaaaaaga atccttatca atgtgatgca ccaaatgctg tacttcattc
     2101 tttatcgaag aaaaattttc attgaaatta ccttcattta tcagctgagt aattttttcc
     2161 aaaatcgaat cataatcaaa cggtttgatt cgatccgttt ttatcttttg atttttcttt
     2221 atcaataaac tagcaatcgg caagctttct acttcggatt ccagcatatt taactgaatc
     2281 aagatctcat ttttcccaat cgttaatggt tcttcttgtg ttcgactata cacatccgca
     2341 caactgattt taatgatatt cttcagctta ccaatattcc cactaaaggg atgagataac
     2401 attaaagaca aggcttctct tgaaacaata atatcttttt gtaaaacatc cgcttcattt
     2461 tgaaaaaata attgaatcag ctctaatcgt tctcgaatgg ggcgatcttc aaaagttggc
     2521 aacataacac taatttgaat acgccgatca aaggtgtcta ataaataatt ttcacctttt
     2581 tccgtcgtgg cgaaaataaa acgaacctta gcagaatgcc aatttacatt ttcaccgact
     2641 ggacgatagt ttccagtatc aataaataga aagagctttt cttgattttc tttcgatagg
     2701 cgatggacct catcgagaaa aagatagccg ttgttggccg ttgccaataa accatcggta
     2761 tccttttcag cccccgtaaa ggcccccttc ttgtatccaa acagtgttgc cgaaagcagt
     2821 tcgggattgt ccgcataatc ggcacagttt aagatagcaa atggtgcatc tttttcaata
     2881 atttgctcat ggatagcgta ttcaaaaatt tttgtggcta aaaaactttt tccaacaccc
     2941 gtggctccat ttattaatac actgagtccg ttaggcggat atttgacagc ggctatacat
     3001 ttttgaatga cctctctcag acttccgtca tgaccaacta cagaatgaaa ggcttcgttt
     3061 atttgctccg tattatctac aaccgcatat tcatctaaag gcttccacaa aacaggcttt
     3121 cccggtaatt tctcgacctt tttatcttcc actaatctcg tcaaataatg gctggcattc
     3181 tgacgagaga tacctagatt cttcgaaatt ttctcggcag tgacagaaga catattccct
     3241 gttagtcgta tcagctcatc aaaaactcga tctttagcgc tctttttcat tttttcctcc
     3301 agtttaatgt gtctaaaagc ataataaact atcggctgca atatgtctat tctatatttg
     3361 tattccaatt tttactttga aacacaaata gtcgaatatt tgcctcagat atctgacaaa
     3421 ataatttatc taccaattta gtttttatct aatatgattt ttatgttttt tcatagctac
     3481 aaatattgaa ctggattcat atattagttt tattaataga ttacagaagg gttaagtata
     3541 tttgcatagt ttccaattac tttatccttc tgtaactttt tcaatcttag atttactagc
     3601 ccgtctcggg ccctatttta caatggtttt gtccactttt ctctggtaga aaaacgtggt
     3661 agaaaaaata aaataacaca aaaaaagcgg cgtttcatcc gcttttggta gaagattgga
     3721 aaactcagat agaaaaagga cgatttttag ctctttagaa cgttgataag tccccattct
     3781 tactgagttt tgccattatc tcctatacat cttgaattct aagaataggt cattatagat
     3841 gcctaggtat ttcgatccta gatctttgta gacttcaaga tgggagatcg tgtcatcaga
     3901 tggatagaat tgttcgtccg acgaaatgtc ttttggcaat aacgcttttg cttcttcatt
     3961 tggtgtagaa taaccaatat actctgcatt ttgtgccgca ttttccggtt ctagcataaa
     4021 gttgatgaaa tcataggcac cttctttatt tttcacggtt tttggcataa cgatattatc
     4081 aaaccaaaga ttggatcctt ctggtgggat cacataatgt aaatgttcat tttcccacat
     4141 catatcggca gcttcgccag aaaatgtcac tgccacggag gcttcttcat tgatcatata
     4201 cattttgatc tcatctgcca cgattgcttt gacatttggt gttaaatgat tcaatttatt
     4261 tgctgcttct gtcagctgtg ccatattctt actattcaaa gaatagccta aactattcaa
     4321 agaaagcccc ataacttctc gtgcaccgtc aatcagcatc aaactgtctt ttaattccgg
     4381 cttccacaaa tcatcccaat gcttaatttg atttgcatca aagaatttat cgttatatac
     4441 gatccctaaa gttccccaaa aatatggaat cgaatactga ttatctggat caaaatccaa
     4501 gtctaaaaat cgtggatcaa tattatttaa tcctttgatc ttcgaatgat ctaatggaag
     4561 tactagtttt tctttgatca ttttttggat catatattca cttggtatag cgatgtcgta
     4621 gtttgtccca ccttgctgga tttttgtgaa catcgcttca ttagaatcaa atgtttcata
     4681 attcactttg tatccatatt ctttttcaaa tttcgtgatc aaactaggat cgatataatc
     4741 tccccagtta tagatggtaa gtacctttgc tccactcatg ccacttgttt tttcaagttg
     4801 acgaacacca agcagcagca ccgcaataat gatgagaata cctaaaaaca gagattgtaa
     4861 ttttttcatc gcagccgtgc caccccctcg cgtactcttc tgtttttctt cagccgtttt
     4921 ggacggtttt ctttactaat aaagtagtat ccaatgacca atatcatgga gaacaagaaa
     4981 actaacgcac ttagcgcatt gatttctaaa ctgattcctt gtcttgcacg agaatagatc
     5041 tctactgaga gcgtcgtaaa gccatttcct gtgacgaaaa atgtaaccgc aaaatcatca
     5101 agagaatagg taaatgccat aaaatatcca gctatgattc ctggagaaag ataaggcaaa
     5161 atgatatttt tgactacttg gaaattatta gcaccaaggt ctctagccgc atcgaccatt
     5221 gaatcgttca tttcctgcaa ttttggcaag accatcaaga cgacgattgg aatgctgaaa
     5281 gcaatatggg aaagcaatac actctgaaaa cctaatccga atcctagtag tgtaaagaaa
     5341 atcaagaaac tagcaccgat aataacatca ggagagacta ataagatgtt attcatgctc
     5401 aacaacgctg tccgtgcacg tcgttttttc gtgtaataga ttcccatagc cccaaatgtc
     5461 ccgatgatcg tggcgatcaa tgcagataag aaagccagca tgaacgtatt caatacgata
     5521 atgatcaaac gtgtatcttc aaaaacagct tcataatttt cccaagtaaa tcctgtaaag
     5581 ctgttcatcg ttcctccttc attgaaggaa taaaaaatca gatagaagat cggtgcatat
     5641 aagaataaaa agacgatccc taaataaaga tacgaccatt taaatttttt catttatgag
     5701 cgacctcctt ttttcttctc gcctgtcagg agcatcacga tgaacatagc aatgattaaa
     5761 atcacaccga ttgtagaacc catcccccag ttttgtgtaa ccaagaaatg ctgttcgata
     5821 gccgttccta aagtgatcac ccgatttccg ccaatcagac gagtcagcat gaataaagaa
     5881 agagaaggaa taaatactgc ttggacacca cttttcacac catttaatga taacggaaaa
     5941 atcactcgtc ggaaggtctc aaagctattc gcgcctaaat ctctactagc actgatcaaa
     6001 gaaggattca tctcttccaa tgcattgaaa attggcataa tcataaatgg aatctcgata
     6061 tacgttgcca cgaacagaaa actgaagtct gtgaataata tctgtttcgg cccgatcccg
     6121 ataaaatcaa agaaatgatt cacgctgccg tgcacactga aaatcccaat aaatgcgtaa
     6181 gctttaagca gcagattcac ccatgtaggc aaaataatca gcatcagcca taattgcttg
     6241 tgcttcagtt ttgttaaaaa ataagcagtc gggtaactga tgatcagcgt gaaaaacgtg
     6301 atcaaaaatg cataccatac agaattgatc gtcatagaaa gcgatgtccc cgacgtaaaa
     6361 taagatagat aattattcag cgtgaattgc ccattcatat caaaaaaaga ttgataaatg
     6421 atcattaata caggcgcgat cacgaataaa ccgagccaca aaatataagg gacggaatag
     6481 atcctgcgca ttgttttcat tcttgtcccc cctattcttc gtagctgtca agacgtgcat
     6541 cgaattcttc ttcagactcg ttgaaacgca tgacatggat atcttctggc tcaaaggcca
     6601 atcctacttt gcttccttct ttagcttttt tcgttgaatg gaccatccat tcattttgct
     6661 gttcatcata acagatgatt tcataatgga caccacgaaa cagctgagta tcgaccgtta
     6721 cgactagttt tcctttatct ggtgtcgtga tcgtcaaatc ctctgggcgt aaaacaactt
     6781 caactggttc atttagacgc atcccgccat cgacacattc aaattgctta ccaacaaact
     6841 caactagatt atcttcgatc attacaccgt ccacgatatt gctctcccca acaaaatcag
     6901 caacgaaatg attgattggc tcatcataaa tatctacggg tgtaccactt tggacgattt
     6961 tccctttatt catgacaaaa atctcatcac tcattgccaa cgcttcttct tggtcatgag
     7021 taacaaaaat aaacgtgatt cctaatcgtt gttgcagatc ccgcagttca tactgcatat
     7081 ctgtacgtag tttcaaatca agcgccgata acggttcatc taatagcaat actttcggtt
     7141 catttacaat tgctcgtgca atcgctacac gctgtctttg accaccagac atttcactaa
     7201 tctcccgttt ttcgtatcca ggaagctgga ccatacgcaa agcatctttt accttctttt
     7261 cgatatctga tttggacatt ttcttgatct tcaatccaaa agcaacgtta tcaaaaacat
     7321 tcatatgagg aaaaagtgca taatcttgga agactgtatt tacttgtcgc ttattagctg
     7381 gcaaatcatt caaccgtttc ccttcaaaat agatgtcccc ttctgtagct tcggtgaatc
     7441 cagctataat acgtaggatc gttgttttac cacagccgga aggaccaagc agtgtataaa
     7501 attttccttg ttcgatctca aaacttactt tttttaatac tgtttcatca tcatagcgtt
     7561 tcactacttg atcaaatgaa atgattggtt tccccacgtt tttaccccct atattacaaa
     7621 tatgattgcg ttgcaacaat caatactttt gttcgttttt tgagatgatt ccttaattga
     7681 tgttgttttg tcgcttgata atagatcgac tgtcctgctt ttgccacata gaccgtctct
     7741 cctaagctta actctaattc cccttctaat acaaaaataa acgtttcaga taatgaaggt
     7801 tcaaacgtct tatattctcc tgcaccttca aaagtcagca agacaggctc catttctttt
     7861 tcattcgaat cagccacgag ccattccaat tcatacccgt tttcttcatc gaaataaacc
     7921 gtatgatctt cttttgaata aaccacttgc agattcgctg cttcttgatg aaaaaattct
     7981 tctggagtca cacctaagac ttcaagaata gaaaagaatg tttccattga tggtgaactt
     8041 aaatctcgtt ccaactggga aatataccct tttgacaaat ctgtgcgttc gcctagttct
     8101 tcttgtgtca aattcttttg gatgcgcaaa tttcttaact tctcaccaat ttccataatt
     8161 tcctccgaat cgaagttttc taatagtaaa cttcctgttt actaccactc gtattagtat
     8221 acgaaattta ttgaaaaagg caaccctttg taaaattttt tttgctgaat atttctttat
     8281 cttttggaat aaattctagt tgtttgattc tttatttttc acctaatttt tgtctaaatt
     8341 tacggaagaa caagaataaa agagtaggct ttatggtatg ataaaagaaa attttaaaag
     8401 gagtgtctca cgaatgaacg cttttcgatc ttttcaggta ataaaggaac ctgttttcca
     8461 tacagcaatc agtgatctgc catttactga gttggctgaa aatgaagtct tggtaaaaat
     8521 tgcctattct gatgtcaatt ataaagatgc attggcaatg agcgagtctg gtcaggtgat
     8581 acgaacatat ccgatgactc cagggattga tttatccgga actgtcgtcc aaagtaacaa
     8641 tccacgtttt tcaaaagaag atcttgtact tgctactggt tttgggcttg gcgtcaccca
     8701 tccaggcggt tatagccaat atcaaaaagt accaggagac tggttagttc ctctaccaaa
     8761 aaatatgacg ctgcgtcaag cgatgatcct tggtacagca gggtttactg ctatgttgtg
     8821 tgtgaacgct ttaatccgtc aaggtatgac ctccgataaa aaagttgtcg ttactggtgc
     8881 ttctggcggt gtaggcagta cagcgatagc cctgttacat aaactaggat tccagtcaat
     8941 cattgccttt tccagaaaag aagagtctgt cacatggcta aaaagtctag gtgctttgca
     9001 agtcgtacga cctgatgaat ttctgccaga gacaacaaaa gctttaggaa aacagcaaat
     9061 cgactatgtc atcgacacag taggtggcga gcagctaagc agtcttcttc cattgatctc
     9121 ttataacggt gcagtagctt tatgtggcaa tgctggggga atcaaactaa acgcgactgt
     9181 cttgcctttt attctgcgaa acatccagtt gattggaatt gattctgtca atgtaccaat
     9241 agatcaacgt ctttctttat ggcaacagat ggcggatttg cagattgcag atgaactggt
     9301 cgtccaagag attactctgg atcaattgcc tgaaacagca tctaaactac ttgctggaac
     9361 acaccaagga agaacactag taaatgtagg tgaccataaa tgaagattct aatcacagct
     9421 ggaggaacga ctgaaaaaat cgatcaagta cgtgctatta caaatcattc gacaggacga
     9481 ttagggcaag cattagctga ttatttagct gccaatccgg ataccacaat cgattacgtg
     9541 actacgaaac aagcactgaa accgaaaaga cactccaata taacgattta tacgatcgaa
     9601 tctgcactgg acttattttt acaattagaa gaactaacga aaaaagaaca ctacgatgcg
     9661 attatccaca gtatggcagt cagtgatttt actcctgctt ttagtttttc agaagaacag
     9721 ctggcaaaaa acctacccac ttcttcaacg caagaagaac tggacaattg gtttgcggaa
     9781 aatgaacaga cagataccac tgtttcaaag atctcttcta acaccgagca tcttgtgctc
     9841 gttttgaaga aaacaccaaa aatcatttct tatctacgtg aatggcagcc aaaagcaaaa
     9901 atcattggtt tcaaactatt agtagatgta ccaaaggaat ctcttctggc agtcgcaaaa
     9961 aatagtctga taaataacaa aactgatttt attttcgcaa atgatttgac ggaaatccat
    10021 ggcgagacac accacggcta tctcttatct aaagatggaa cagtggaaga agctcaaagt
    10081 aaatctgaga ttgctgcatt gattactgag aaaattagac tggaggaatc aaaatgaaaa
    10141 agatcttact aggcgttact ggaagtattt ctgcttacaa aagcgctgat cttacaagtc
    10201 aattcgtcaa attaggtgcc caggtagatg tgattatgtc taaaagcagc acacatttta
    10261 tcacgccatt aaccttgcaa tctctgtcga agcgcgcagt ccatacagac gtcatgcaag
    10321 aatcaaatcc gcaggtaatc aatcatatcg aactagcaaa acaagctgac ttgtttctca
    10381 tcgcgcctgc tacagcaaac attatcggca agctagcaaa cggcattgca gacgatcttc
    10441 tttcaacagt cgcaatggcg cttccagtgg gaacaccaaa attgattgcc cctgccatga
    10501 acacgaatat gtatcaaaac ccgatcactc agcgtaatct ggaaacattg agatctgtcg
    10561 gctatcagga aattgaacca agagaatctc ttttagcttg tggagatttt ggaaaaggtg
    10621 ccttagctga tattcagacg atcatcgaac aggtcatgct gactcttaat gaaagaaatg
    10681 cttagaaaaa aggagtactt atgaaaaata caaaaaactt tacattaact gcgatgtttt
    10741 tagctatttt gattttatta gctgttacgc ctttaggttt tatccctatc ggtccaatca
    10801 atgcaacaac catgcatatt cctgtaatca ttgcttcgat cgttttagga ccaagactag
    10861 gtgcttttct tggcggtaca tttggactga tcagcatgat ccgtagcaca gttatccaaa
    10921 cacctttgtc ttttgtcttt tctccgttta tcccagtcat cggaacagaa catggaagct
    10981 ggaaagcctt gctgatcgcg tttattcctc gcatcttgat tggggtggtt ccttatttcg
    11041 tttataaagg tctgcgcaaa ctaatgaaac aaaaagcaga ttccgtttct ttattcttag
    11101 ctggactttc cggttcattg accaatacga ttttagtgat gaatatgatc tactttttat
    11161 tccagcaaga ttatgccaaa gtcatcggaa caaatttaaa tgctgtttat tcagctattc
    11221 tagcggttat ttttacaagt ggtatacctg aaggaatcgt cgcaggtctt gcgactgcag
    11281 cagtatgcaa tgtcttgtta agatacttca agcacccttc tcttactaac tgatagtcat
    11341 cgtagccgat ctctgatttt tttctctttt ggtgttacta ttgaatggaa agatttgacg
    11401 tcgaaaggat gatccaaatg aaaatttttg ttgtaggtgc aaacggacaa attggccgac
    11461 acctgataaa agatttagct ccaagttccc atgaaatttt tgcaggggtg agagatgttg
    11521 cttcccaaac attagtaaaa aaagagaatg tttcgtatgt ctcttttgat ttaacttggt
    11581 ccgtggaaaa aatgaccgaa gcattcaaag gcatcgatgt attgattttt gctgccggct
    11641 ctcaaggaaa gaatcttctg caagtagact tggatggcgc aatcaaaaca gtgatcgcag
    11701 ctgaaaatgc gcatgtctct cgctatctga tggtcagtgc tgtttatgca gatgaaccag
    11761 caaaatggcc tgagtccatg acagattatt atattactaa acattatgca gatgaatggc
    11821 tgaaacgaac taacttagat tttgtcattc ttcaaccagt cacattgact aacgatgaag
    11881 aagttacttc cattcaatta actaagccta atgagaaggc atcaaaaacg atcactcgct
    11941 ctacagttgc tgctgtactt gccgctctag tggaagaaac ggatatttct cgtacgactc
    12001 tagtactttc tgaaggtagc aaagagttga atacagcatt ccaagaatgg gcaaaggagg
    12061 aatagatcca tgcgtgccat ttcaatcaag catcctggtt ctgtagatgt attagaacaa
    12121 attgaagtgc ctgttcctaa accaaaacaa ggcgaattac tgatcaaagt ccatacagca
    12181 gctgtcaatc gaacagatat tatgaaaaga gaatcagtct ctttgaaacc tccctaccct
    12241 attttgggag tcgaagtagc tggagaggta gttgagaata aaagtgatat ctccactttt
    12301 actcctggga caaaagtata cggattagtc aatctaggtg gttatgccga atatgctgtg
    12361 atgcctgccg atcgtgcaat ccttctccct gatacgctag actatgtttc tgctgctgga
    12421 atctcagaag tttttttgac tgcttatcaa acactttact ggcttggaaa attacaagaa
    12481 ggcgaaactg tcttgatcca tgcaggagca agtggtgtgg gaacagcagc gatccaatta
    12541 gctagacagc tttctcacgc gaaaatcatc gttactgcag gatcagaaga aaaactcgcc
    12601 ttttgtaaag aacttggtgc agatgaattg atcaactata aaaaacaaga ctttcaagaa
    12661 gaggtacgaa aaatcactga aggtcgggga gtcgatgtta ttttggattt catcggcgct
    12721 tcttactggg aaaagaattt agcttctata gccgttgatg gacgttgggt cttgattggt
    12781 atgctaggtg ggaccatcgt gcctgagata gaccttggtg tattgattgg gaaacgtgtc
    12841 caactgcttg gtacattatt gacacctaga agtgatcagt ataaagcaga gctcgcacaa
    12901 acttttatgc aaaaagctgg gccttttcta gaaaatggca agattcggcc aatcattgac
    12961 cgtacattcc aattcagcgc agtcaaacaa gcgcatgaat atatggaagc aaacaaaaac
    13021 atcggaaaaa tcattttgaa gataactgaa taaaatcatg ctgatcaaaa aagaggctgg
    13081 gacagaagcg tttaactcca agaaataaga agaaattcac gaaaattgct tttcaaattt
    13141 ttgtgaattt cagcttattt ccgaaggaat ttcttctgct tccgccgttt atacgtgttt
    13201 agagcgtgag acaaaagtgt tctttacttt tgtcccacgc tctttttccg aataaacggt
    13261 gttcaaaagc ttgcatctgc ggtaataagt ccaaccaagt taaaaacata agaagctgtt
    13321 tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct cttcactaaa
    13381 cagtgttcaa tcagctgacc ctcggtatta gctcaaaacg ctgatttcac aacctctttt
    13441 tatttacgtt ctatttcatt cattttccga agcaaatcga gctgcttgta tgcccgcttg
    13501 tcgcccaaaa ataatgatct ccgcaactga atttccaccg atccggttgt ttccatgtaa
    13561 gccacctgtg acttcaccag cagcatataa ccccctaatt ggctggtttt ctttatctaa
    13621 tacttcagtg ttcgtattga ttttcacacc gcccatcgtg taatggattc ctggtgcgat
    13681 tttgatcgca taataattcg gagacgataa gtcatgttcc atggctgtgg cacggttgaa
    13741 ttgagcatct tttttctccg caacatccgt attccatcca tcaactgttt ttgctagtgt
    13801 ttcttttggt aaatcgattt tttctgctaa ttcttccatt gtctttccag ttgtgacata
    13861 tcctttttgg tcataaaatt caatagctgt ggcacgatca cgcacgcctt ggtcaaaaat
    13921 caaataagct gatttttcag gcaatgctgt aattgctgca gatactttat cccttgtatc
    13981 catttcattg acaaatcttt tcccctcttg atcgaccaag attgctcctt ctccgcgaac
    14041 aacctcacca atcaagaccc cctcatcctg ctgcacagtt ggatgaattt gtattttatc
    14101 catatcaacg gtttgtccac ctaatttttc aatcatggtg ataccatctc cagttgatcc
    14161 ttcttgattt gtcgtaatat aatcagctaa atcgggacga tatttttcaa taaattcttt
    14221 gctggcacca aaaccgcctg tggtaactac gattgctttt ccggtgacct cttttggttc
    14281 ttctccttga acatgtacag tcacttcatt gaccgtttcc ccatttttct taatgtctgt
    14341 cacatctgcg ttgacaaaaa tagggatttc ccgctcatgg acgttattca ataacccctc
    14401 cactaagtat ccaccaatag ccgagccatc agacggacga tgtgtcctct tttcactcat
    14461 ccctccagtg atcgttaagt tgtccagttt gataccgttt tgatctagcc aatcgaccgc
    14521 atcagcagag tgatcgacaa agtatcgaag catttcttta tcattcgttc ctccaccacc
    14581 ttttagcgtt tcttcataga aggcatcatt cgagtctttg attccttctt ctttttgaaa
    14641 ttttgtttct gaagcattca taccgcttga tgatttagat gtgtttcctc cggcaacagg
    14701 catcttttcc aaaattactg gattcaaccc agcatctttt gcttcgatag ctgctgccat
    14761 ccctgcacca ccagctccaa taatcacgac atcataagat tctttcattt cttttggatc
    14821 tgtatatact tgttcagaag cgcctgatga tacttcggca gattccttcg tactagaact
    14881 tgtctgcgct gtatttttct gatctgcgcc gcatgcgctc atagcaagaa gtgcaacggc
    14941 tgttaataaa ccagcaacta attttttcat ttgtatcccc cttttctcct tatactatat
    15001 taagtgattt ttttcacaat atcaactctg tttttcttta agtcatatag ctttggtaaa
    15061 cacacgcttc ttagtataat taaggatata catgaaggag ttattttatg aatcatttga
    15121 aaaccttttt tacgaacagt acaaaaactg caggtagaat cacaaacgga atctatattt
    15181 tattacgtat catcgtcgtt attatgctag tggatcaggt ctggaaccaa cactggggaa
    15241 atacaatgct tttattactg acattaattt tatttgaaat cccttatttt ctggaaaaga
    15301 tattaaagat acagattcca aatatgctag aaatcatttt gttatctttt atttttagtt
    15361 caacagtttt aggagagtta agtgattttt atggctattt ttcaatgtgg gatacttttc
    15421 tacatgcatt cagcggattt ttagctgggg gtgtcggctt ttcccttgtt tatttgctca
    15481 ataaaaatac aaaaaccatg aacttgactc ctttattact agcagttgtt tccttctgtt
    15541 tttcgatgac agttggtgtt atgtgggagt ttgtggaatt ttcagcagat aaattactgc
    15601 aattcgatat gcaaaaagat acgatcatcc agcaaatcaa tagtgtggag ttaagtaaag
    15661 acggaaacac tgtctatcat gttccagata tcactcggac aatcattgaa agtcaaaatg
    15721 cagatggtaa aacagataaa acaatcatcc aaaatggtta cttagacgtg ggaatcatgg
    15781 atactatgaa ggatttattt gttaatttta ttggagcagt tgttttcagt gtatttggtt
    15841 acctctatag tcgaaacgat taccaaaaat ttacgttcat caagaatttt attcctaaaa
    15901 aaatcaaacg ataaaacatg ctaaaaaaca agagaagggg cagtgacaat acttttgtca
    15961 ctgccccttc ttttttgaat gtatggcatg ccattagtta tccgatagta ttaactcaaa
    16021 aaagtaagat atgaggaatg actatttcgt tttttatccc aatactgagg acttatttca
    16081 ccatttcgtt atttatctaa tttacttaaa acctgcataa ctttttcata atctggatta
    16141 ggtgtgccgt ctagattttc agcaataaaa gtaaaaccca tttttttata aagagcaatc
    16201 gcctgatggc tccatgtctg tgtgtgaaga tagattggtc cgtccgtatg gagggtagga
    16261 aattcttgca agactctggc agtcaatgct tttgcgagcc cttttccttg gtaatctggt
    16321 ttcactgcta accaatgaaa aaccggatac acttgctctc ttgatttttt tcgccaagcg
    16381 gtacaggttg ctactttttc tccttttttt gtctctacaa aaaacatcct tctttcaagt
    16441 tcttcaggat aaggagcaaa gttttgttga aaatactcaa gtgcttcatc tgtcgtttga
    16501 aattccccaa cggaagtctc aatctccgcc caagcagatt catcttcttt ttgataaaat
    16561 cgaaattcaa aaccttccgc taaaggcgtg attggaacat tttgttgaag tggtcttgtc
    16621 atccagattt ctgcgtaagg taacgtttta tctaacattt ttctatccct cctcgtctcc
    16681 attatgccaa ataacatatg actcagctac ttatttgact ataacagata gagatataaa
    16741 tcagaaccag tcatctgcgt caaattcatt tcgattagcg tatacccatg ttcctgccat
    16801 acctcgcatg aaatctccta aagcagaaga aggaatacct ccaccaggtg cgggtgttcc
    16861 cggactagga agtggtactt gcggctgcag atgctcatcg gtggaatcaa tagttgacaa
    16921 gcgttttttc ttcgtttcct tctggtcatc tgattccttc tgatcttccg actgtttagc
    16981 aacaaattct ttttctgatt gttgttttat tttaggctct aaaatttcat tttccaatcc
    17041 agaaacttgt ggggccaaat gagcttcttg ccttatcgtt ttattagatt ctgagatttt
    17101 tctagatatt cttgctaact ctaggcttct tgtttgtttt actttccctg ttggagaaac
    17161 tgcatgagcc atttgttgtt ttttttctga caagctttct tcttgtcttt tttgttcttt
    17221 ttgatctatc tgtttgttta atggtaaaac atgtaatagt gactgagcat ggagttcttg
    17281 gagtgaatac gggtcatgat aactatacat aatcgtaaaa tcacctgttt gtttggtcaa
    17341 ataaccatcc tccaatggta cttcattgat tgtaaccgat acatccttta acggtacggg
    17401 tttacctaag gaatttgttg ctgattggat caactcttct ttgtttattt tttcgtttgg
    17461 atgaacaata agatcatgac cttctaatga acgattgtct ggtatataaa cataagtaaa
    17521 ggtaatttcc cctcctgatt gcttcctatt attcatcagt tctgagtgaa gtacttcaag
    17581 gatctccgac cagtgacttt ttctgtctaa tccgtttgct tgcatccaat cagaaataga
    17641 agtgactttc ccattttgtt cataagtcga taaatctata tttggcactc gatcagaaaa
    17701 ttcagcaggt aggcgcaaat caggaatacg atttacacta ctcatatgtg tttctgcttg
    17761 aagttcttta tttgtttgac tatctataaa atgagtgatg acagtcgatg aagcagcaga
    17821 tgcttctaca taaactacag ctgttgtttc tgccacattt gatgttttcg agtctatata
    17881 agttaataca acgggatatt ctcccggagt tgtagtatct actgtcccgt tcattttgat
    17941 ttcttcttta tttcctgaag ttccatcgct ttttgttaca tattcaacaa gatctttagg
    18001 ggcgtaactc tctcctgttc ttaagtgaag atcttttcct ttgatagctg tttgatcagc
    18061 acgaacatgg atcacagcag atgcagtcat tgtttttaaa gcaattggat caaaatagga
    18121 caaggaaata gtagatgttc ctgtattagt cgtatccacg atattcccat tgtttgaaat
    18181 tactttggac atatctcctt gtgttccatc tgcgttcttc agcctttcta ccagatcaga
    18241 tatcgaaact tttgtccctg ccagaacatc gattttcttt ctttcaaact gtgaataatc
    18301 tttttcatat acataagaaa cttgtgcgct tccaccgtcc tttatatctg tcaaagcatg
    18361 attcaaaata tcagtgatct gttttaactg atcacctttt gtaggatcag tcatatcgga
    18421 aaagatagaa agcggcacac tgcttccggt ttccgatgtc cataatgttt tgttaggaat
    18481 taaccagtag tggtttatcg ttggaggata actgacttta ttataggaag tcaatgcagg
    18541 aagtagctgt tttccatttg catcagtgaa agtatgagta atagtgatag gggaatcagt
    18601 ggtctcagaa acagaggtac gtacgacctt gatggtttgt gtcgttgcct gaacgggtac
    18661 atgcatatag acacctacag ataaactgat cagcatcgtg cgcatcataa acttaacgta
    18721 gtgtttttca gaacacgacc gttccttatc taaacgacaa gcgtgtttat ttagttgttt
    18781 tgaacgtctt tttgaacaca tttattctta gtcctcccta ctcttgtcta aacatgaagt
    18841 agtaattttc ataagatgat ttcattatat aaataaaatc aaaagaagat ggactattag
    18901 gagagtattt tttttgattt tttattgttt caagaaaatc ggaataataa cttatgtact
    18961 ttttaatttt gttaagtaat atatctagtt atatctagtt atatctagtt accttttctt
    19021 tgaaaaaaac ataaatcagc taataaatat gtgagacaaa gctgccatcc ataaatacta
    19081 gctgattttt tcagttaaat gtctattttt ttctctgatg atggtatttc caaaatattc
    19141 ccagtcatct ggagtcggtg gatttttcca aattatctgt tctttattaa atttttcaag
    19201 taaacaatca gataaataaa tattcgtacg agaagtgatt cgactctcaa tcacaatatt
    19261 gtaattttta aatcctttca cttgagaaat aatgtaatcg gtataagcag atccttgcga
    19321 aaaatcaata cagacatgaa tcggtgcttc aaaatatgat accggacaaa taccgaccaa
    19381 taaaaagaga taatcataaa ataagcgaat catgaaacgt tcatcttttc ccaagaaata
    19441 gcgtttattc tcttgtatga ccttttggat tgctgtactg aatattggat acgtctcgaa
    19501 gaaaaactgt agctgctttt tagacgtaaa gctagtcatc tcaaagtcga atatccaatt
    19561 ttttcgattg acatgcatca tacccatacg aagttctttt cgtatatata gtctttcttc
    19621 ttcagagatt tcaatcgaaa atggtaattg atcaatcaat tccactgaca ttttttgact
    19681 aatcatgtgg atatcatgaa aatgagcttc cgcaagtcca aatgcgccat ctaattcaaa
    19741 ttcttcttcc gtgatcaaaa acaacatgat ataagaaatc tcatcaatga tctgctcttt
    19801 agggatatga agaaaagaaa tatgtgataa atccttgatg atcttcattc cttctgagct
    19861 cttaaagtat tcatctgaaa agaaacgttt tccgattggt tgattcccat aggccctcaa
    19921 caaactaata gtggttagca aattgaattt gattcgtttt gttgcagaca gttttaaatt
    19981 gaacagatat tcaaaatatt gcacgatact ctttgcttca ttcatgacgc tttctctaaa
    20041 tggcatagaa aaaccattaa agaccccgaa atatacccca aataatacgt tccgtatgtt
    20101 tatttcttcc ccaactaata tgtttttctt taactttatt tgatgctgtt caagaaaaga
    20161 gactaattct cttcttttta cataggctct tgatatgctt aaaaaatgat tctctgcaaa
    20221 tttttcgatc gttacttttg aatccacgat ctccgttaac agtcgagcaa tttttgatcg
    20281 ttgaaagtag attcttctta aatttttgat atccgtcaaa ttcagacgtt cgacatggat
    20341 ctctgcttct tctgaaattt ggatctctgg ctttgttttg aattcagtaa attctttatt
    20401 taattctatg atataatttc ttgttttaaa tttagacaaa cctaatgttt catataactt
    20461 aggcagcact acataattct ttttctcaaa ttctaaatat ctagctagca gcagtttttc
    20521 tctatcttcc tcttctataa ggtaatccaa cttcatatcc tacacctcaa aaaatgcctc
    20581 ataccaatta cttgatatgc agatgaaagt tcatcttttc acctatactg attttatgat
    20641 ggattatgct tgttttttca tcagttattc tcatttttta tctttttttt gagaatattg
    20701 caaaaaatgg aaaaatttat gaggaaatat tctatatttt ttgttttatt gtgttaaaat
    20761 gtacaagatt tgcgccattt gcgtaaacaa ccacaaaaaa gagcaggacg gccaccgtcc
    20821 tgctctttta cttattttaa gttccattac ttattaattg gctaaacttc tgcttgtttc
    20881 tgatattgct tgtttttctc caatgatccg cacttctgtt tctaaagaca catcaaattt
    20941 ttctttgatt acttcttgga tgtgcgcaat caattcaata taatctgtcg ctgtcgcatg
    21001 atcgatattg acgatgaatc cagcatgttt ttcagagact tgtgcaccgc cccatttcag
    21061 gccttgcaat cctgcttgtt ggatcagctg acctgtatag tgtcctactg ggcgcttaaa
    21121 gacacttccg catgaagggt attctaatgg ctgttttgat tgacgcaatt cggtcaactc
    21181 atccattctt gctttgattg cttcataatc ccctgtttgc aaactgaatc gagcttccaa
    21241 tacgatagca cgtaattctt ggactttact gtgacgataa gaaaatacca tttcgtcttt
    21301 tgtcaaagtt ttcaatgttc catcttcaag caaaagatcc acttcagcaa aaacatcttt
    21361 gatctctccg ccatacgcac ctgcattcat ataaaccgct ccaccgacac ttcccgggat
    21421 gccgcaagca aattcaaatc cagtcaatga ttctgccaaa gctgcataag tcgtatcaat
    21481 cagtgaggca cctgcttccg caatgaccat cgttcctttg acatgaatct gtttcatttc
    21541 agtcagcatg atgactacac cgcgaatccc gccatcttga acgatcagat tgcttgcatt
    21601 tcctaatacc atccaaggta tatcatggat tcggcaataa tcgatgattt gctttacttc
    21661 ttcttgtttt ttcgggaatg caaggacatc agctggtcca cctgttttcg taaacgtata
    21721 gttcatcaat ggttcatcaa acaatatagt aatcgtggct aagctttcaa ctatttcttc
    21781 tttattcact gaggattttc ctttcttttc tcaaacgtcc tcattattct atcacgctta
    21841 atacaatctt gccagtcttg ttcaaagttg atgacggtag tgatctttaa gcacttcctc
    21901 acataattca tgacttctta caacttcttt ttgtttaagc aattctgttt ccgatttacc
    21961 taaacggatc tgagaaaaga aggcctctac cattggctca aacccgcgtt tgactagcgt
    22021 gtttgtccag tcgccaaatt ctttcacttt ttttccatca gcatcttgaa tgacaagctg
    22081 ggtcagatcg tttacttcat aagtaccatt tttacttgtt acttgatatt tttctatatt
    22141 agcgccactt gctaaatcca tcgttaaaat agccgtttga ttttgtgttt ctaattgtaa
    22201 aatggcatat tccaaaaatt cctttgtttc tcttatctgg tattttgttc tcaaaatggg
    22261 ttcatctaat aaatacacag ctgtatcaat aagatgtaaa aacaaatcat aaaggacgaa
    22321 ctctgtcgtt tcttttgccg ctattcgatt tttttgcagg tggatcaacc gcttatctgg
    22381 gatacgtttc aattcttcta ccataggagc aaagcgccgg ttgaaaccga tcatcaaaat
    22441 tatttggttc ttatcagcta attcttgcaa ttcacgaact tcatttagat ggacactcag
    22501 tggtttgtca atataaacat ggataccatt ttctagacat tttttggcta attcataatg
    22561 tacttttgtt gctgcgtgga tcatacatgc ttcgattttt tcttctaaaa gctcatcgat
    22621 cgtttcatat agatgttgga acccgtactc tttttgaatt ctttcttttg tttctttatt
    22681 tcttgttgca aaataaaaat caagtgttcc ttgttttttc gcataagttg gtaaatatgc
    22741 tttttctgcg atattcccta caccgattac accaatcttc atctactttc ctcctggatc
    22801 atttagttac tttcatctta caccattttt tactaaaatg aggtacaata gtatcgcaaa
    22861 ggtaaggaaa ttaattcgaa ggagtgtgct tttattgaaa tttatctctt ggaacgtaaa
    22921 cgggcttcgt gccatcgtaa ataaaaattt tttagaagtt tttcatgagt tagacgccga
    22981 ttttttctgt ctgcaggaaa cgaaactcca agcaggacaa attgatttag atcttccagg
    23041 ttattatcaa tattggaatt acgcggaacg aaaaggctat tccggcacag cgatttttgc
    23101 taaaaaacca gcattgaatg ccacttacgg aatgggtatc gatatccatg atacagaagg
    23161 ccgtctgatt actttggaat attccgattt ctttcttgta acctgttaca cgccaaattc
    23221 tcaaagcgaa ttaaaacgtt tagattaccg attagaatgg gaagaagcct tttataatta
    23281 tttggaaaat ctaaaaaaac aaaaaccagt catcgtgtgt ggggatctaa atgtcgctca
    23341 tcaaaagatt gatttgaaaa actggaaaac gaatcaaaaa aatgctggat tcactccaga
    23401 agaaagagcg gctctttctc gactgttgga taacgggttt atcgatacat tccgttattt
    23461 ttatccaacc caggaaggtg tctactcttg gtggaactac cgattcaatt caaggaaaaa
    23521 caatgctggt tggcggatcg attacttttt gacgagcaaa gatttagagc caggactagc
    23581 tgatgcaaag atccatacgg acattatggg cagtgatcat tgtccagtag aattagattt
    23641 gaaataaaga ggttgtgaaa aagtcgttta gtttcgagta ttaagaaaaa ttttgtgaaa
    23701 atggctttcc acattttgca taaatttggc ataataccga gaaactggct tttgaacact
    23761 gtttatcgag agattgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa
    23821 aatagttcct catatttttc gtttggttgg gcttattacc gcagatacaa gcttttgaac
    23881 accgtttatt aagaggttgt gaaaaagctg cactgcatca agtaataaga acagccaaac
    23941 aaaaatagtt cctcatattt ttcgtttggt tgggcttatt accgcagatg caagcttttg
    24001 aacaccgttt attaagaggt tgtgaaaaag ctgcactgca tcaagtaata agaacagcca
    24061 aacaaaaata gttcctcata tttttcgttt ggttggactt attaccgcag atgcaagctt
    24121 ttgaacaccg tttattgaga agttgtgaaa tcagcgtcct gacctgagta ttagagaaac
    24181 aaatggcaaa atagttcctc atattttgct attttttgag ctaataccga gggtcagctg
    24241 attgaacacc gtttagtgga gaagttgtga aaaagtcgcc tactttcgag tcgtaagaaa
    24301 tttttttgaa aatggttcct cacaactttg cataaatttg acttaatacc gagaagtcat
    24361 gatccccatc taactccaaa tcacaaggaa tgagttcaaa aactagcttt tctcaacaaa
    24421 tattacataa aaaattctat gttttttgtt aaatttattt gcttttttca aaagtatgtt
    24481 acagtaattt cccaaggagg ttttacagaa atgaattttg ttgccatgga ttttgaaact
    24541 gccaatcatc agccttatag tgcttgttct cttgcgctag tcatggtaaa aaatagccag
    24601 atcgttgatg aattctatac attgatacag cctgagactc cttttttctg gcgaaatgtc
    24661 caaattcacg gaatccatca agaagacgta cgcaatgcgc cgaaattccc agaagtctgg
    24721 caaactatcc aaccatattt tcaaaaaaat cgtttagtcg ttgcacataa tgctgctttt
    24781 gatacaaagg tcttagcggg atgcttagat tattatcatc ttgaacagcc taattatctt
    24841 tctttatgta cagtgaaaag tagccgtcgc ctgtttcctg aatttccaaa tcatcgattg
    24901 aacactgtgt gcgaaaattt gaacatccaa ttagatcatc accatgatgc attggaagat
    24961 agtcgtgcat gtgcggaaat cctgctttat caagaaaaaa aattcgggac agatccattg
    25021 aaaaaacttg tgactataaa gtaaaagaat acttatgaaa cacacaaaga ggtcgtgagc
    25081 ctgccgtttc actccgagtc acaaggagca atttcaaaaa atagcttctc atgtttatta
    25141 aatattgtga ctggtgaccg aggagctggc aggagaacac cgtttattgg agaggttgtg
    25201 aaaaagctgt tctgcatcaa gtaataagaa cagccaaaca aaaatagctt ctcatatttt
    25261 tcatttggtt ggacttatta ccgcagatgc aagcttttgg acaccgttta ccaagaggtt
    25321 gtgaaatcag cgttttgacc tgagtattag agaaacaaac ggcaaaatag ttcctcatat
    25381 tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgttt attcaaaaaa
    25441 aatggactgc gacacgactt ttgtcacagt cccttcgtgt gtttttctgc cttctcaaaa
    25501 ggatgacagt ttctcactct tttcctattt cttctattca atcaaataac tcattttata
    25561 tacatctaaa aattcaccta gatctgttct tgcccctcta ggcatgaccg cttcgatttg
    25621 gaagcccatt tttcggtaaa ggtgtacggc tctttcatta cgaacttgaa cgtcaagctc
    25681 caatcgaaaa agtacaccca tttccttcgc ccaactgatg atttcttcta gcataagagt
    25741 gcctagtccc attccccaat attcctgcag aatactgata ccgacttctc ccacgtgaga
    25801 caaacgaaat tgactgtctg ccttgacaga tgctgttcca ataatcgtcc cttcatagat
    25861 tgcaagcagc aataaattat tattgctttc gtatatataa tccaactctt cttctaaggt
    25921 ttctggtggt aacaggagtt ctgcttcatc caaaactaaa aattctgtct ccttattaac
    25981 ttgtcccatc atcaaaagaa tgtcttttgc atctcctggc actgcttccc gcaagatgat
    26041 cccaacttct tcactcatga ttttcagccc atttttctag aattcgctgt ttgaaggttt
    26101 cactttgctc cccgtatgag attagtttca cataccgctt ttctaaatct gtatcacttt
    26161 tttctaaaat cagttccaaa taatcctccg gcagtgcttc ttctaataga tttccccatt
    26221 caacaacgga aagcccatca ccttcaaaat attcatctaa ccctaaatct gctccgctag
    26281 ctgctacccg ataaatatcc atatgataca acggaatccg accttgacta tattctctaa
    26341 taatcgtgta tgtcggactt ttgatcattt gcgttatccc taatccttgg gcaattcctt
    26401 tcgtcaacgt tgttttaccc gcacctagat caccagtcaa tatcagattg tcacctggca
    26461 ttgcggaatg accaattgcc tctgcgaatt gttctgtcat tgttaatcct gatagttcga
    26521 tcatttttca tcttctcccg ctcttctttc tttttgccag tatatcgcgt aatgaagttt
    26581 ttttaaagaa aaaatgaact agtcaagaac agcagctgct gtttttggct agttcgcgtg
    26641 tatttgttaa tcaatcaatg tttgtgctgc tgtgataatc gataatttat atacatcttc
    26701 ttcatttgct ccacgagaca aatcagaaac cggtttgttc aagccttgta gaattggtcc
    26761 gattgcttca aagttaccga aacgctgagc aattttatag ccgatatttc ctgactgcaa
    26821 ttctggaaat acaaagactg ttgcttgacc agctacttct gaatctggcg ctttcagttg
    26881 tgcaactgaa ggaacataag aagcatcaaa ttgcaattcg ccgtcgatca tgtattgtgg
    26941 cgctagtttt ttagcgattt tagttgcttc aactactttg tctacttctg gcgatttcgc
    27001 tgagcctttt gtcgagaagc tcatcattgc tacttttggt tcgatatcga acaattctgc
    27061 tgtttttgca gaatctacgg cgatttcagc caattcttgc gcagttggat tcacgttgat
    27121 cgcacaatcc gaaaagacat atttttcttg atctcttcca cgtaccatga tcatcgctcc
    27181 gcttgtccga ctgatgccag gctttgtttt gatgatctga agtgctgggc gaacagtgtc
    27241 gccagtcgaa tggatcgctc cagagaccat tccgtctgca atccccatat acgtaagcat
    27301 cgtaccaaag taattcacat cttttaaaat cttttctgct tgttctttgg atgcctttcc
    27361 atttctacgt tcaacaaaag tatccaccat ttcatcccac ttatcgtaat tatcagggct
    27421 gatgatcgta aagctagatg ttttgattcc tctggcatgc gctgcatttc ttatttcttc
    27481 ttcgttccct actaaaatgg gttccacaag ttcttctgct ttcaaccggg cagctgcgcc
    27541 taaaatacga gcatcagtag cttccgggaa aacgattttg atcccacggc gaacaatttt
    27601 aaatcttaaa ctatcaaaca attccacaag atgacctcca gacgttttta tatgtattat
    27661 catacactat tccagaaaaa aagaacagtt tattttatct gtatcagtca aaaatgctgg
    27721 aaaaattaag tttttcacat acaaagcgaa ctgcactata acaggaaagc gctgttttat
    27781 tacactgttt caggcagttg ccaatcaatc ggttcttctc ctaacgcaag gagcgcatca
    27841 ttcgttttcg agaagggttt ggaaccaaaa aagccacgac ttgcagataa cggactggga
    27901 tgtggagatg ttatgatgat atggcgactc ttatcgatca ttttgatttt ttcttgtgcc
    27961 ggttttcccc acaagataaa cacgattggt ttttcccgtt catttaattt ttcaatgatc
    28021 gtgtctgtta atctttccca acctttcccg cgatgagaat acgcttgccc ttcccgaacg
    28081 gtaagaacgg tgttgagtaa aaggactccc tgtttagccc atgacactag atttccatgt
    28141 tgaacaggtg cgattccaag atcatcataa agctctttat agatatttct taacgatggc
    28201 gggatcttta cgccaggttg cacagaaaaa gataagccat gagcttgatt ttctccatga
    28261 taaggatctt gtcctaaaat caccacttta acttcttcat aaggagtcaa ttccaatgct
    28321 tcatagatat gatacatatc tggatggatc ttttgggacg catattcttt ttttaaaaat
    28381 tctcttagat gcagataata atcttttgaa aattctgagg acaaaacttc ttgccaactg
    28441 ttatgaatga tttctttcaa aaactccacc tcctaataaa aagaataact cacattttct
    28501 tcaatcacaa gggacaatct tcaccctcag cacttgatta atatggtaaa ctaggaacaa
    28561 gaagcagaaa ggaacgataa tcattatgat caaattaatt gcatcagata tggacggcac
    28621 attattagat gcccatatga gtgtttctcc tgaaaatgcc gaggcgatac gttttgcaaa
    28681 tgatgccggt gttgagttca tggtcgctac tggtcgaaac tatcaagaag ctcgcgcagc
    28741 tttagatgag gtaggaatcg attgtgcgat gatcactttg aacggggcac aggtatttga
    28801 taaagaagga aattcattgt ttaccgtgtc gattccaaat aatcaagcaa tctctgtttt
    28861 agatattcta gatgctaacg gtatttacta tgaagtagca accaacgatg gtctttattc
    28921 tgaaaatcaa gcgaaacgaa ttgaaagttt tgcttccatg gtggctaccc atttacctca
    28981 ccttacgtat aaaatggcga ttgccatggc ctcagctaag ctggagttat tgcacatcac
    29041 atacgtggac agtattcgtg ctattttgga agaaaagaat ctggaagttt taaaaattat
    29101 ttgttttcat acagaagggc cagctgtatt aggtccagtt ggaaagcaga tttctctttt
    29161 aggcgatcta gccgttactt cctctggtca aaacaatatc gaagtgaacc ataaaaacgc
    29221 tcaaaaagga attgctgttg cccatgtcgc tcacgagcgt gggatttctt tagaagaagt
    29281 catgacgatt ggcgataact tcaatgatgt cagcatgctg caagtagcag gagtcagttt
    29341 cgcaatggga aatgcagaaa tcgaagtaaa agattatgcc aaatatacaa cggacacaaa
    29401 ccttgaatct ggcgtaggaa aagccatatt gcgcgcgatt gcagaagact tgtagtttta
    29461 tctagaaaag agggatatag atgtcagaat ttttcattca ggaaaagcag cttggacgtg
    29521 caactcggac aattgtcaaa aacgaaaaag gacattccct gtttttgatg gttggtcgct
    29581 ggggaacaaa gggcgatgca ttgtctcttt atgccatgaa tggcgatctc gttgctcaca
    29641 tcaagcagat aagcctgaca ttcggtacgc gttttgagct gtataaagag ttcgaaaagg
    29701 tgggaaccat gcagaaaata ttcaattggc ccggtgactt ttattatatt cgtagacttc
    29761 actggacagt ccaaggtgat atctataatc atcaatatca aatccatcat ttcaatcagc
    29821 aaatcatgaa aatggataaa gcaacacttt tttcaggaga ttactatgta ttggatgtct
    29881 caaatgatga agatgctcca gtttgtattt gtattgctgc ggttttagat tactggctct
    29941 accgtaaaga caaaagtcgc gataagcctg tttcttaccg acttaactta agttaaaaag
    30001 atgtacctga aatgcggaaa tacgcatttc aggtacatct ttttagatat tcttttgcat
    30061 atgatcataa agatgaccac tgatcatccg ctgaccgaca actttgaagt catggcgttc
    30121 gtataatttt ttagcctttg gatttttttc atccacactt agaccgatga cggagcgatt
    30181 agctttcttc gccaatttag gtaatgcttc taataatcgc gaaccgatgc cttgtcctct
    30241 aaaatcttct gatacactga tagagtccaa ataccattcg ttgggaaacg tttctttatc
    30301 aatgaacaaa cgtgtagacg catctagctg atatttctct aagataggta ccagtggctg
    30361 atcgatgatt gattcttctt catctgtata accgtaagca actcctgcaa ctttaccgtc
    30421 tatttcttct acgatacctc gtgaataact gtaacgataa tttggatcag tgattgcttc
    30481 ttctaaaatc tctaaagtct tttgttcccc tgtctgcttc aaaaatggca gttccatatc
    30541 ttttagaatc actaagatca atggggcgat ttctggagca tcttcttttc tcgcaaaacg
    30601 aatcatagtt tcctctcctt ttctccattc taacatgcag aaaaagaaat cgcaaaatcg
    30661 ttattttaaa aaatcttcaa tcttttcagt aatgaaataa ccatgagtaa cgattcttgc
    30721 cgcttcagtt cggcaataag ggcagacgac tggcatttct ttatcattat gttcgactac
    30781 attattgact tcttcgtatg caaatttttt tccgcaatta gggcatttca ttttttccat
    30841 gttatctcaa cttcctttca tctttactct cttagcttac tcttatattg taaaaaagag
    30901 aagcaatatc cttcttctat gcagatatcc atacctgacg gtcctgttct ccatctatta
    30961 atcggacttt ataataagga cgttcatctt tgactagaag tgtccactct ttcacttttg
    31021 cttttttccc gtagtagctg actgcttcct tcatcgctgc ttgcggttgt ttgaactctt
    31081 ccgttttttc tatatcaaaa agatgttctg cggccttttc cgtgtcattt cgtacggttg
    31141 ttttcccagt ggatggattg actgctatga cttgtccttc acaaaaaaac tggtattcat
    31201 atgttgcatt cgtaggattc tccaaatctt tttcaactgc aaaatgaata gcagatatgt
    31261 tatgttcttt tgtttcgttt tttacgattg ctgctgcttt tttcaatgag atccgatatt
    31321 cctggtcact aatatcatcc atgtgaaaag cggcattgag cagtcccgtc ataccggtaa
    31381 tccctattac gatgagggca gcaacaccaa acatcatttt tttcattctt cattctcctg
    31441 tttctttttt ttaaagaata tcaagaaatg aacgaatgaa aaatcgaatg aaacgaaaaa
    31501 aaagtgacaa ttttgttatc tttattcgtg cagttctaat ggcaatccgt ctggatcaaa
    31561 gaaaaacgtc attttttttc ctgtaaaggt atcttctcta atcggttctg attcgatccc
    31621 taaggactgc aattctgcaa ttgtttcttc aatatcgtct actttgaaag caagatgacg
    31681 caagccacaa gcttctggat agtttggtcg ttgtggcgca ttagcaattc caaaaatctc
    31741 caattcagca cttcctagtt tcagatctaa tttatagtca tctcgttctt tgcggtaatt
    31801 ttctctgatt acttcaaatc ctaatttatt aacataaaaa tcacgtgatt tctgataatc
    31861 tgaaacaatg atggctatat gatggatcgt tgtaaggttc atatgagcac tcctattctt
    31921 cgtttaagct cggatctata ttaatctcca gttccattgt aaatgtgtca gattgaaaaa
    31981 aatagctgtt tttccttttt ataagaaaaa aggcaaaaaa aacagaaagt aggtcctcca
    32041 agacactctt cctgttccca atataataaa tgaaagtaag aaattatatt ttcaagaaaa
    32101 caaacagaaa attatcataa cacaaattat ttataattga aagactagtt ttttttcaag
    32161 aaaagcatac aatagattgt gttagtaaat acagaacaca ctacatttag tgtcttaagg
    32221 agtcctcata catgaagtca tattttcagt ttttagctca tcagctaaaa ggctggccgc
    32281 aacaaaatta ttatcttttc tttttcagtc ttggctgcca ggtcatgact cttgtcaata
    32341 gtcccatcac ttggctgtcc gttattacat ttatcggtac cacgcttggt gtattgtgta
    32401 ttttatccat caatgcggca aaatcagtca acggtatttt aggtattttg tctgcgattt
    32461 gttttattat cgttgggttt tcagcaaaga actatttaag tatcggtgaa cagattgctt
    32521 atatgatcac attggatatc cccgtattgt taagtaaaga atggaatcat aatatggctt
    32581 caaaaatccg aaaattcact ggaaaaagct ggtttatcgc aatcatcagt actttccttg
    32641 tctatcttgt ttcaggattc ctgattggtc gttatacaga tgatcctcgg ccgtgggtag
    32701 atgccattgc tttttctatt agcttgactg ctggtatcat cagtttcttg cgttacaaca
    32761 accaatatta ctggtggtta gcttcagggc ttgctcaaat ggtcctttgg tatatttcat
    32821 ttacgcatgg atctgcaagt ttggcgatgt tcgtgaacag ctctgtttac ttgctgaatg
    32881 atgtccttgc atttaccgtt tcaccttggt ataaccaaaa agaacgtgaa cgtatgcaaa
    32941 aaatggaaga agaatactac aacgcacaac aacacgcata aaaaaactga gcgtgggaca
    33001 aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga atcagaagca
    33061 actccttcgg aaataagctg aaattcacaa aaatttgaaa agcaattttc gtgaatttct
    33121 tcttatttct tggagttaaa cgcttctgtc ccagcctcta ttttttatag taattccttc
    33181 agatactttc ttgcctcatc attgaaacgc agtgattctt ctaacttctg ataatattcc
    33241 aacgcttgtt tctgagcttc agtaaaacga taatgttttc ctttccattt gtcgattgtc
    33301 tcttgcacat ttccagattg gatatctttc actactagtt gccatttttc ttttgagact
    33361 gtacggtctt tcttatcagc agattgtata gcttctcgtt ctttacgcaa tacttttaaa
    33421 aaaccatttc tgactatccg aggaatgctg tcgaagaacc cttctgtaaa accatagcga
    33481 caatattgct tccaaataaa tagatagatt tttggttgga tgaatcgttg gtttttcgca
    33541 aaatattcca tctgagcagt gacttctggt gttcgttgac gttttgtctt tctcaacacg
    33601 gaaataaatg tttcaaaata agcattagga tgtactggca cttcttcata tacgatggat
    33661 tgccaatctt tacgtaaaaa tgcgcgatag ttgatcaaga attcttctac atcctttact
    33721 aattcttctg caaagtcgtc aaatagttct ttttttatct gtcgcttttt tgactgccca
    33781 ttgatgtgtt cacttatttt cttgatttca ttttccaatg cttgataatc cgctatgtaa
    33841 atcggcattt ctacttggcg aaattctgcg agccgctgag ctaattttat atccggtaaa
    33901 tatggctgtt tcatccgacg attttggtac aagacaaaac agtatttttg ccatagtcct
    33961 tcaggaaaat cgatattctt ctgcttaagc cataataatt cgtctaaggc cagatcaact
    34021 tttgtcattc cctgatggat cgaagctgcc actgtcggag agacatgcag gtgatcggca
    34081 aagtggttga tgcctagttt taggatcttt ccggtttttg gcgatttaac gatgtattgc
    34141 acttttaatt ctctaccaca ctcacaatac aatttttgct ggcttcgttt tgttcgaaaa
    34201 aacggattga tcttgaagtc agaaaacacc caatctgttg cagctaaata atgttcttcc
    34261 ataaaacgag agctgattag atatcggcgg tgcttatcga ctagttcccg ctgttttttc
    34321 gttaattttg catagatctc ccaacgttga ctgcttgtgt attgtttaga aattttgatg
    34381 ggatagcttt tgctgatttc ttttggaaac cattcttcaa agacggattg ccattcttcc
    34441 acggctgttt ctttcctttc taattacaaa aagctggcta ctaacataac gttagcaacc
    34501 agccagtgtt tcttgctaaa ttagtgagtt gttccggcat ttccggaaat atcttcttta
    34561 ttttctactg ctgcttcaac tgcataagtc aaagaagaag caatgtcttc taaagacata
    34621 gaaggcgtac ccactggacg atcgatcact tgatcgggtg agaatggcac gtggatgaac
    34681 ccacctcgaa ttgccgggaa ttgtgtttcg atcaaatgaa gcaaacgata catgatatca
    34741 ttgcagacaa atgttcctgc tgtataagag atgtacgctg gtagaccatg atcttgtaca
    34801 ttttttgtca ttgctttgat tggcaatgag gtaaagtaag cggctggacc gttttcttct
    34861 aaagccgtat cgaatggctg atttccttca ttatcaggga ttcttgcttc tgctagattg
    34921 attgcgacac gttcgaacga gactgctgac cgcccacctg cttgtccgac acagatcacg
    34981 acatctggtt ggtgctgttg gatggcttct ttgactactt gactgctttt ttcgaatacg
    35041 gtcggtactt ccactttgat gatttcagca ccgctgattt catctggcat tttttttact
    35101 gcttcatagg ctggattcac tttgtcccca ccaaatggat caaaacctgt tactagtact
    35161 ttcatctgtt gttcctccgt ccgattgatt taacatatgg ttatctttta tcataccatt
    35221 ttccttgaac agcgtaaata aaggaaacaa tagtttaaat aacataacag atcaaactta
    35281 atatattctt ataaaaaaat agtgttccac tacaatgtta gaagaggttg cgacaattat
    35341 tctgtcacaa cccctctctt ctattattta aatagaatca tataagcaat ttggaaaact
    35401 agcataaata acgcaataaa caactggttt ttgatcactc cgtatttgtc tttcatctcc
    35461 agcatcgcta caggcacgat attgaagtta gcagccatcg gtgtcagcag ggtcccacaa
    35521 aaaccgcaag tcaatgcaac cattcctatt aacgctggat tcgctccatg actaaatacg
    35581 aatggtgcac cgatcccaac cgtcatcacg gtgatggccg caaatgcatt ccccatgatt
    35641 accgtaaata tcaccatgcc aagagcgtaa atgatgatgc cgatgttcac atttccttct
    35701 ggaacgatcg tacctactgc attggaaatc actgtcccaa ctccagcgct tgtaaatacc
    35761 gcacctagag aagctaaaag cattggcaac atgcttaacg gccctactgt gcccagcata
    35821 tctgctgcat catctaaaaa tgtacttggt cgattatctc gagaaaagaa catcaagatt
    35881 agcatggctg caaaaacacc gacgcccacg ccgactaaag cacctaaatt ggtaaataga
    35941 gcaaacaaga tcgcaaagac accgatactc aaagctggaa taaaaatttt cattcctagt
    36001 ttgtctgaca ttttttccat atattctttt gatggaagcc ggctttctcc tttgctgact
    36061 ttcttagcaa ttgctggtat ggtcatcgca aagatcaagc caccgttcac gattgccgga
    36121 agccatcttc ctcctgcaat aactacccct aagaccgtcc agaaaaaggc tgacccataa
    36181 cgatgaggat tggttttatc tgttaaattt cttactccag tgtaaatcaa tacgagtccc
    36241 atcacgatat aaaggatctc cagtaattta tcgcctaata aaatatcagg atttgtaaaa
    36301 aagtccatct tattgcccct ttcctggata atatttcttc gctaatttct tttctttaag
    36361 atagaagtag acacttgcca caactaaagc cgtgatagcc actggaactt ccactaaagc
    36421 aagctgtccc aatgttacgt catatcctag atctttcagt gttgattgga ctagcaacgc
    36481 ccctgctcct ccgatgaata gtacttggcc aaagaaccag cagacatttt ccatagctga
    36541 agccatacct tttagttctt cttcatgctt aggatggact tctaaccctt ttgattcgat
    36601 ggtccccaat gccatcggta aaatgattgg acgaacaaat ccagcaactc caccaaaact
    36661 cacgttaaaa gccgcaaaga tcattcgaaa gatcccataa atatctacaa tcataccagc
    36721 agatactttc ttgaatcgct tgatcaagga agccgctgat tctttcagac catttctttc
    36781 aagcgttcct gttgccaaca taatgatgat aaaaatcgcc atcccgcgat tgtttacaaa
    36841 acttgtacct aaaagttcta acattccttg aatgccaaga ccgccagtca aagctgtaac
    36901 aactaaagca accattacga tcaatatcga gtctaattta aatgcaaacc cgagaataac
    36961 aatcaagacg cctaaaaggc taagataatt ttccatatat tttcctccat aaaatgacaa
    37021 tattttttta attataaagc aaaacacata aaaaatatag ttgttttctc acgaaataga
    37081 cactattttt aaatattttt cgttattttt ttattatata attcacctta cttattgtaa
    37141 gataaagctt tttttaattt attgcctgct aatatataaa aataaggtct agttactgtt
    37201 tttttaaatg attaataaat aacaactcct ttctatgaaa ttgcaaagca ttcatcttct
    37261 tttctgctaa cgatatatcc gtcaatgctt gttacacttt actaaaaagt atgagattgc
    37321 cgatatctgg ataggtgtgc tatactacag aaaaagatat acgatctgcc accgggtagt
    37381 aaaaaagagg atgatcattc cattaggtct ttgaagggat gattctcctc tttttgtgta
    37441 ttttttatat tgaaggagga acgtatgtat ggcatggatg gaattaatca tagcaggaat
    37501 tttggaagta ttctggtcaa cgatgatgaa atggagtgat ggtttttcta aaatcaatta
    37561 ttctttttac acagtgattg gaatgattgc tagtttttac tttttgtcaa aggcaatcaa
    37621 aagtttgcct atgagtctct cttatcctat ttggacaggg attggcgcag ttggttcggt
    37681 cattattggt gtggtcttgt ttcatgataa gctgaatctt tccacttggt tctttgtcgg
    37741 gttgctgctc atcagcatca ttggcatcaa aatcactagc ggtcactaga tacttagaca
    37801 agcagacaag gactgatcag aaaaagcggg gctttttatt tgatattcct ctttctatga
    37861 aaaaacaaga aaataagact aatgacctag cgacttctct actcatcagg tgtatgcttg
    37921 tactatctat agaaaaggag tgaatatttt tgaaaagtct ttcggtccaa gaattatcca
    37981 agaactttgg tcacaaacaa gccctcagcg atgtcagttt tagcttatct cctggcaaaa
    38041 tcgttggatt agtaggtcct aatggcgctg gaaaaacaac gatcatgaaa gctattttag
    38101 gactgatcca ttactcagaa ggaacgatcc gtattggtga aaatgagatt tctccctcta
    38161 gtcataaagg attagaacaa gtaggagcat tgatcgaata tccgggtatc tatccattct
    38221 taacaggtta tgatcatctg aagttattct ctgaaacaaa cgatgtatct gctatcgaca
    38281 ctatcgtgaa acagctcaag atggaaaaat acattcacaa aaaagcgaaa tcctactcgc
    38341 tgggaatgaa acaaaaattg ggaattgctc ttgccttact gaaccatccg tctatcgtta
    38401 ttttagatga gccgatgaac ggtttggatc cacaagcaac aagagatgtg cgaaaaatca
    38461 ttaccgactt ggctactcag ggcacctctt tcttgatctc cagtcactta ctcagcgagc
    38521 ttgaaaaaat ggtcgatgag ctgatcctga tcaatcaagg aaaaatcatc aaacaatgta
    38581 caatggctga attgaaccaa acagcgcaag atttcatcgt tatctctacg acaaatgatc
    38641 aagctgctcg aacgatcttg acagacgctg gatatccctt ggaagatcct gataaaatca
    38701 agcttctaaa aaaaaacgaa gagttattag gaactgttat ccagttattg tctgcagaaa
    38761 atgtccatgt gacagatgtc cagcatctga cgaatgattt ggaatcttcc ctattggaaa
    38821 ttctggattc agtaaagggg gaagaagaaa aatgacgttg cttataaaac aagaattatt
    38881 taaattgatc aagaaaaagt caactgctgt tttatctgtt ctattagttg ctttgctcat
    38941 aggaacagca cttctagcca aaaaatacac gacgatcatt gatccagttg aaatgactgc
    39001 tcagcttttc agtgccacat cttggattgt ctttatcatg attgctgctg caagtacgat
    39061 catttcaatg gaatcacaat atggtacatt gaaaaatctc ctttatcgaa agtattctcg
    39121 aggagaaata ttagtaagta aatggatcac attagttatt tattctattt atttatacct
    39181 tttagctatt attgtcaccg tgctgatgaa actgatcctt ttcccttcga tttcctttac
    39241 ggagaagatt tctactggtc agacactgat ccaaagctta ttcacttata ctttgggtag
    39301 ttatatcggt ttatggctaa ttttaagttt agtgttgatg attgcttgtt ttatcaatag
    39361 ttccggtgcc tccatctcag cagggatcgt cttttatttt gcttcatcaa tcatatctgg
    39421 tatcctcatc gcactcattc aaaaatggga gtggatcaaa tggaatccaa tcagtatgct
    39481 gaatctgcaa aaccaaattg gcaacgaaga gattatgaag caattgactc atctttctac
    39541 taatcaaatg ctgtttggca atttagctta cattgctttg ttcttagcat tgggatacct
    39601 cgtattcaag cgaaaaaatg tatgatggtt cattaacaaa gaagttgtga cagaagtggc
    39661 cagtttcaag aaatttcggc ttatttttag aagaagctac ttcttgaaca ccatttattc
    39721 aaaaaaagaa gcgatgacac gaatgttgtc accgcttctt ttttgaataa accacaggct
    39781 tcacagcctc tctttatact tctatacgtg aagcatccca gtggcaaata aaaccattgc
    39841 gattactccg accatcgtca caccgataag taaaatctta tctaagtgga tatgttgtac
    39901 aggttcgttt tgttctttac gtgctttgag gtaaaaataa atacctggta agaaaagaac
    39961 aagaacaagc atcacttcat taaaggctac aactagcatg gcccatactt gaaaagcact
    40021 tgctaaaaac cctactaaaa attggaccca ctctttccgc tgataagaaa gcttcatttg
    40081 gtacagccct aagaaaagcc acgtgaagaa aatcgctgct gtaccaagtg tataagcaaa
    40141 gttatatgca ctgtcagtga ataataatga aagtaaaaag acattcgtac ataacgtcgt
    40201 caatacgata gcataagttg gtgccccatt tttgttgagt tttccccatg ctttaggcaa
    40261 aagtccgtcc tcagccatat tccccattgt ttcaatcggc aagattgtcc atgagagcca
    40321 aaccccaact aaagaaatga tcaaaccgcc gttgatcacg accgcccccc atttaccaac
    40381 catcatttca aatacatatc ccatagcagg ttgcgctttg atcgtttgca gctgattcat
    40441 cgtcattgtg ccatatggaa gcatggaaac caagacgtaa ataatgatca aaccgaatat
    40501 tcctagaatc gttgctttcc caacatccga ttgttttttt gctcgtgaac ctacgacaga
    40561 agctccttca ataccaacga atacccagag catcaccatg atgcaactca atacttgact
    40621 agaaaacggg atcggctgcc cattcgcatt caatatgata tcgctagaaa agttattaat
    40681 gaattgttgg atcgaaaagg tgctgatagc tactacaata aataaaaata atggcactaa
    40741 tttacaaaat gtgacgatag cattgacaaa gctagcattt tcaactccac gattcactag
    40801 ccatgcatat ccccataaga agatcgtact catcacgatc gatgccacat tttgaccacc
    40861 ttcgaatacg gggaagaaat aacctaatgc actcatcaac aatgttgcga acgccacgtt
    40921 tcctaaccag catgtgagcc agtaacccca accgctgata aaaccagcga aacggccaaa
    40981 accttcttct gcgtaactaa agatcccgcc ttttaaatct ggacgttttt tattgatatt
    41041 attgatcgat aaggagagca tcaacattgc tgttcctacg agtacccagc tgaagatagc
    41101 tgggccacca gccgatccgg agatgtccgt cataatacca aatacgccac ctccaatcgc
    41161 tgaaccaatc actaaggcag caagtggaat caaaccaatt ccttttttct gttgctctgt
    41221 atccatgtaa gcatcctcca aagtaaaacc gcttttatca aaaagtataa caaacctttt
    41281 ttatttttac ttaattaata aaaactttaa tagaaatata attctaccga cttttttctc
    41341 attatacaag caaagcgcct agtccctttc caaattacac caccaaaaaa acttaaaaag
    41401 atgctatact aaagggaaag aattggggga tgatccatga acattttata tatatcgatt
    41461 tcaggaaata cgcgtgcttt tgccaaacat ctagcagaat atgctgaaaa aatgcatgca
    41521 gaagatcctg tcaatcctga agttacttta aaagaaatcc atgaaaatag tgattttacg
    41581 aaagaaacag aaccattttt tactttcgtt cctacttatt tagacggcgg gaatgggtta
    41641 gacaacggtg acacagaaat cttgaccgaa acgatgcgcg aatatctgga atacgaagac
    41701 aatcataaac tttgccttgg tgtagtcggt agcggtaata aaaacttcaa caatcaatat
    41761 tgtctgacag ccaaacaata cgcacaaaga ttcggctttc ctttcttggc agattatgaa
    41821 ttacggggaa caccatctga tgtgacacgc gtttatcaaa ttttggcaga aaaaaataaa
    41881 taacacaaaa gaagaagctg aaaaataaat ttcggcttct tctttttatg ttctctattg
    41941 tttctattct tattaagctt tgacatccat cacaggtgct tttgctttcg ctttacctgt
    42001 tttttccaag acaaagtttt ttacacgatc catattggtt accaagacga tcatggaggt
    42061 ttctttgtct gctgctttga ttgcttctaa gtcagcttct gctacaagcg tatcaggtgt
    42121 cacttgctgt ccttctgtta ctttcacatc gaatggcgta ccgcctaagt caactgtgtt
    42181 gatgcccatg tgaagcaaga tttctaagcc atttggtgtc gtaatgccga tagcatgttt
    42241 tgttgggaaa acagaactta ttactccagt aaccggggaa tagatatgtc cgttttctgg
    42301 ttctacaaag taaccttcac ccatcatttt ttgtgcaaaa actgggtcac ttgctttatc
    42361 taaagaaaca actgttccgt cagcaactga tgataagata gtcacacttt cagctgaagc
    42421 ttgaggtact gctttttcag gtacttcatt tactgtggct gtttcggtcg ctagttcttc
    42481 tttttctaaa cggtctttag gaataccgaa gaagaatgta gcaacgaagc caccagcgta
    42541 agcagccaat aaaccaagga tatagcctaa ccatttacca tcagagatca atggaatcaa
    42601 agcggctcct gaaggtccaa tagcgattgc tccgacattt ccgatcattc cgatcactgc
    42661 cccaccgata cctccaccga tacaagcagt aataaatgga cgtcctagag gtaatgttac
    42721 tccgtaaatc aatggttcgc cgattcctaa gattccgact ggtaaagccc ctttgatcat
    42781 ttctgttaat tctttgtctt ttttgcagcg gatccataat gccaatgctg cacccacttg
    42841 tcctgctccg gccatcgcaa ggattggaag caataaggta cgaccagttt catcaatcat
    42901 ttgtacatgg attggcgtca agatctgatg tagtccgaac atgaccattg gcaagaataa
    42961 tgtacctaga acgaagccgg caaatgctcc tccaacacca agtacccagt tgatcccacc
    43021 gaccatgccg tcagaaatga accctgctaa aggcatgatg aagaaaattt cgatcagacc
    43081 gatggcaatc agggaaagag ttggcgtaac gatgatatct accgcatcag gtactacttt
    43141 atgcagtttt ttctcaacga ttgacaataa ccaaactgcg aagataacac ctaagatccc
    43201 gccttgtcca gttgcaagag cttcaccggt aaacaagttt ttcagtggtg cttctggatt
    43261 catccctgtc agcatcacaa cagccccgat aactccacca agcgttggcg ttgcaccaaa
    43321 aacttgtgca gcattgatac ctgtatagat aactagatag ctaaacatcc cgtttttcaa
    43381 gatgttcatc acatcgatat attgttgcca tgttcctgcg tctagtgttc cagctgtgac
    43441 taagtttgct aaaactgcgg caatccctgc cacgatccca gtgccgacaa atgcagggat
    43501 cattggtaca aagatattag agatgtcttt taataccgat ttgattttag agggtttttg
    43561 tttcgatttt tgtgccgctt tcatttcttg tgcccgttcg tttacacggt cttttccaga
    43621 tgcagaagaa cttgctgtag ttgcactagc agaaggaagt ttttctccta atttcacgcc
    43681 tgctaagtca accatttcct gcgctacttt gttcactttc ccaggtccga tgatgacttg
    43741 gagctgttca tcctctacta cgccaagtac tcctggaatc gcttttaatt ccgagacatt
    43801 tacaagatca tcattgatga ccttcatacg tacacgagtc atgcagtgga caatgctttc
    43861 aacattgccc ataccgcctg ctccttgata aatttgtttt gccagttctt ccaaagtcaa
    43921 ttcgtttttt gccatggtct tttccccctc ataaggttcc ttttacaaaa ccgtttgctt
    43981 cagctaattt tgcttgagct gcctctttat cactatttgt caaaatcatg acgatagcca
    44041 gtttgacatt ttgatctgct tcctcaaagt acttttcagc tgtttcgtag tcagcttctg
    44101 tcgcttgcat gatgattcgt tttgagcgtt caattagttt ttcatttgtt ggtttgacgt
    44161 cgaccatcaa gttattgaat actttaccga taccgatcat actgatagta gaaatcatat
    44221 tcaaaatcag tttttgtgct gttcctgact tcaatcgagt agagcctgtc aaaaattctg
    44281 gtcctgcatc gacttcgatt gggaaatccg catgctcgct gatttctgct tgtttattgc
    44341 aagaaatcgt tcctgtaaca gcaccaatcg attttgcgta atctaatcca ccaatcacat
    44401 atggtgtacg accgctagct gcgataccaa taactatatc attttttgtt aaattcagct
    44461 tgattagatc ttcttgccct aattcataag aatcttcggc tccttcgacg gctacagtca
    44521 ttgcttcttc tccaccagca attaggccta tgaccatttc cggttctaca ccgaatgtcg
    44581 gtacacattc agcagcatct aatacaccaa gtctaccact cgttccagct cccatataaa
    44641 tcaagcgacc acctttttta aaggattcaa tcgtgttggc gatcactggc tcgatacttg
    44701 gcagttcttt ttcaacagca gcggccactt tctgatcttc tttgttcatg agttggactg
    44761 cttgagaaat actcatttca tctaatccaa aagtttcttg gtttctgcgt tccgttgtta
    44821 ggttctctaa attgattttg ctcattttta tcttccttct tttcttataa attgatagtt
    44881 cataccagct ttgatttggt tgatcagcgg tagttcttct ttgatgactt tggctactac
    44941 attcactttt tcatccgctg gcaaatcata tttagttaat tggatctcac ccatatagcg
    45001 caaatacttt tcgttatcta aagtaataga tcctttcata cgcttagctg tatttcttac
    45061 tgggattggc ggaatctctc gaaaacgcgc atctgcgctt cgtatgacat ctcttgcatc
    45121 atcttgccga ttcgtatgtt ttcctaatat atattcgtaa aaaacttcat ctacaggttc
    45181 cgtatggagc aagactgtct gttctttttg gaaagaagca aattgctcct gaacgtgttc
    45241 tgacagacct gcatctccga tataaacaat atccgtgtta cttgctaata gatcaagagc
    45301 acctgccaga ggatggatcc ctcgatgttc ttccaaagtt ggtaatcctt gatacagtgg
    45361 acctctcaac tctgtatctc ctggtacaaa gccttgtaca aggaatcctt gagttttgag
    45421 ccattgattt ttttcctgat accattcttt gtccaaccct gtttcaagac gtggataata
    45481 attatgccaa gcttcaatct gtgagaaatc agccgctgct tctttcaact catcgatatc
    45541 attaggcgtg atcgtgctgg cattcaaact gattttcaat tgatgtgacc agtcggcgat
    45601 ctgctgatta gaaatatgat aatccattct taaccctgtt acaccaattg ctttcaaggg
    45661 tgttagatct tctaatgaaa agccagctcg tttcaatgcc tctcctgaaa tatcgaccat
    45721 caactctagt ttattctttt tcgcttcgct tcctagttcg atcaatcgat tttgataagc
    45781 cgaaacatca tcttccggta tatgcatgga agtgaaaata ccgacaaagc cattggatgc
    45841 catttggcgg atatactgtt tcttctcttc tgacaaagaa tcattcataa atattgaaaa
    45901 gccaaacata ttatccctct ttttctgtat ccgtcttcat ttctatgact agagtatata
    45961 tctaaagaaa caaaatttca atacttttgt taaaataatt aaattttatt tctaaaaatt
    46021 ggatttataa tgccaaaaga attgacacag ccacaaaaaa ggcactgcta agcaaaagca
    46081 gtgccttgaa acattttatt taaataacac aaaagtctaa ttactcataa gaaattaatt
    46141 ctgtattgtc taccatgaat gtaaagaacc cttcattagg cattggatga aaataagcat
    46201 tgatcactct tatcaaggca ttatgacaga ctaacaagta gacattttct tcgtcttcca
    46261 tgcattcttt caataaagga acgatccgtg cgtacacatc taagaccgac tctccatttg
    46321 ggaaacgtac agcaaacgct aaacgcgctt tttgaaagtt ttcatctttg cttggcagtc
    46381 cgtcataatc cccaaaatcc atttctacca atcgctcatc agcggtcatc ggcaaattga
    46441 gacgatcaac tactgcttgt gctgtatctc ttgctcgttg aagaggggaa tggatgattt
    46501 tcgtgattgg aacaggcaaa tctactacct tttctgctag tgcctgtgcc tgcatctgtc
    46561 ctttttcagt cagcggcaca tctgctcgcc cgcaaactaa tccttgtgcg ttccaagttg
    46621 tttctccgtg tctcgttaca taaagcatgt gattctctcc ttcgttgaca aagttctcct
    46681 taaccatacc ttgttgtctt tgagattaca aggtttagat tttctcaaga gaaataatct
    46741 cagtagatac tttatctaga aaggtttgat catgttccac tagtaacata gcaggtttca
    46801 cgttcaaaat cagttgttct aattgttctt gattgaagac atccaaataa ttcaatggtt
    46861 catcccatat atagagctca gctggttggg ataaagattt agccaattcc acttttttcc
    46921 gttgtcccat actcatctgt tcgattttat tatgaaaaac atctctttcc atcccaagct
    46981 ttcggagatt attcaaaaat gcttgatagt ctactcggtt tttcttcgcg aattccgcca
    47041 atgtccctcg attgtcttca taattttggc gtacgtaact aatggtcaaa tgtttgggct
    47101 gggatttttc tcctacaacc ttgccgttga atgtccccag aagataatgg ataatggatg
    47161 actttcccgc gccgttagga cctgaaattg ccatccgctg atgaggttcg atcgtaaaat
    47221 gaactggctc gaataacaga ttttcatacc ctaattgaag attttctacg cttaacagcc
    47281 gtttatgatg agacacttgg ctattcatcg tcaaagcatc gatatactcg atatctttta
    47341 ataatttttc tttctcactg atctgggttt ccatccgatt cacgattgct ttagatcgtt
    47401 tcatcgtccg tgcagcatca gcgcctactg ctcctttatt cactcgccta gattccgtat
    47461 cgatgaatcc gacttttttc tttgttttgt ccccttctcg ggaacgagac cattctgctt
    47521 tttcagctgc tgttttcttt agcctgctga cttctttttt caatttttca tttttagcta
    47581 tttcaaattc atcacgaagt tttttctgtt cttcatagat agagaaattc ccttgataaa
    47641 gttccagttg gctcttttcg attgctaaaa catggtccac tacttcatcg ataaatcccc
    47701 gatcatgact caccacgatg aatccttgtt ttttcttttt caagtaagcc gctacttgtt
    47761 ttctgccaga gatatccaaa tgattcgttg gttcatcgat taacgggaaa tgagtgtcat
    47821 ccacaaataa gagtgccagt aggacttttg tcttctctcc tcctgatagt gtgctgaatt
    47881 ctctccataa gatttcagga tctgtttgca tcagttggag ctctctttca atttcccata
    47941 tctcaaaatc cataatctca tttaacacat aataggtcaa acgctcttta tcttttgttt
    48001 gctgcgggaa atagacaaac gcttgctgat ggatcacttg cccttgataa gggagtttgt
    48061 tttgcaggat attcagtaaa gttgtctttc ctcgaccgtt tcgtccgata agtcctagtt
    48121 tccattgtgt gtcaaagttt agattggctt gttcaaataa taatgtgcct tggctgtcat
    48181 agccgaatgt cagatttttt atttcgattt tagacattat tccatcacct cgtaaaaaaa
    48241 gaccagtcct attttcagaa ctggctaaag aggatgcaag ggttggggca cacaaaaaga
    48301 cccatacatc gaaagaacca agccaatccc gaaaatctgc acactgctcc cttgtctcca
    48361 caaggtacag tctgaatgca gctttcatca atcgacttaa tttttcaatg cacggatccc
    48421 tcactttgtc ctatatttga aattacgata gcataaagaa attgtttttg tcaactactt
    48481 cgggagaatg ttgaaaaatc tgctgagtgt ctgtcctaag atttctgttt tttctaatgt
    48541 ttgcctgtgt tccattgctt cctctaaatc gattggtcct aattgaatgc ttaagacaat
    48601 ggggagaaaa tcaaagagtc cttccagctc tcggtctcta cttccgcaaa tagcgattat
    48661 tggttttcga tatttttttg caagcactgc tacgccatat ggaacttttc cgtggattgt
    48721 ttgctgatca atccgccctt ctcctgtaat gatgacatcc gcttgttcga tctgtttttc
    48781 taatccagtg atttggctga ccaactcaaa accagagacc atcttcgctc ctaacaaaaa
    48841 aagggctccg ccaataccgc ctgctgctcc gcttcctggg atgtcatcta aatccacctc
    48901 ccaaacatct tttatctgct gagcaatctt ttcagcttgt ttatccaaga aagctaacgt
    48961 cgtatccgtt cctccttttt gtcttccaaa ttgccaagca gctccttgtt gacctgtata
    49021 tgggttggtc acgtctgcag cgatgatcac ttttacttta gaaacaagaa cgttccttcg
    49081 atccaaatga agttttctag tagataacaa aggatttcct tctgtgtacc cttctaaaga
    49141 agcacccaaa gattggagta atcctagacc accatcgcta catccgcttc ctcctaaagt
    49201 gacaatgatt tgttccactt cttgaagcag tgtatctttg ataagctctc ccaaggcata
    49261 actagaagca agtcggatgg tttgatccgt tggtttatca agtaaatcta atcctaatac
    49321 ttgtgcagat tcaatgactg cggtattttt tccttgccag ttcgtcaata aataactgat
    49381 ttttcgctga cgaaataaga gatccatccc atcaatcgta cgccaggttc ccccgagcgc
    49441 gtaatagatt gcttctgcag tattttctcc gccatctgca atcggcacat tgatttttct
    49501 tcctttacgg tcttcccaac catttagaac tgcctgattt aattcaaatg aagaagcact
    49561 tcctttaaat gaatcgattg ccgccaaaat atccatctat atccctcact ttcttcttaa
    49621 gtaagacact ttttttcact tttttcaaac aaaacaatgc tattttcttc tgtttttcta
    49681 tatactaaaa gggaactcat tgaataaagg agagaaaaaa catgaaaatc gcaatagctg
    49741 gagccggagc aatgggcagc cgaatcggat taatgttgca tcaaagcgga aatgaggtcg
    49801 tcttgatcga tcgctggcct gctcacattg aagccattcg tacaaatgga ttgattgctg
    49861 actttaatgg caaagaagta gttgcaaaac tgccaatcta ttcaccagaa gaaatcatcg
    49921 aaagcaacaa acacgtggat ttgatcgtag ccttgacaaa agcgaatcaa ttagatgata
    49981 tgtttcgttc gatccaatcc attattactg agaatactta tgttttgtgt ttactgaatg
    50041 gattaggtca tgaagatgta ctggaaaaat atgtaccaaa gaaaaatatc ttatttggga
    50101 tcacgatgtg gacagctggt ctagctggtc ccggaaaagt cacattatta ggtgatggag
    50161 aaatcgaact agagaacttg gaaccagaag gcgaagcatt tacaaaaaaa gttgtagaag
    50221 tctttcaaga agcaaaattg aatccaatct atagtcataa tgttcgttat tccatttggc
    50281 gaaaagcttg tgtaaacgga acattaaatg gattatgcac gatattggat tgtaatattg
    50341 ctgaattagg tgcgcaaaaa gcggcagagg gcatggttcg aacgattgta tctgagtttg
    50401 cttctatcgc agcaaaagaa ggaatcacat tagatcaaga agaagtgtac caacatattg
    50461 cttctactta tgatcctgac aacatcgggt tgcattaccc atctatgtac caagacctga
    50521 tcaaaaacca tcgtttgact gaaatcgact acatcaatgg tgccgtttgg cgaaaaggac
    50581 aaaaatatga tattgctaca ccatattgtg cctttttgac ccaattagtc catgcaaaag
    50641 aaggaatcct tggcgcagaa taaaaggctc tctgatgaga atcagctgga tcagcagctg
    50701 gttctcattt ttttaccccc atgaaagttc aaaaagaaaa acacaattct catgttgcca
    50761 taagaattgc gcttgtttat tttcatctat gattggttgg cacttttatt tatttttaac
    50821 agaggaaaga tcgtattcat attgttcata aatagcggag tcagtagtga aaaaacgttt
    50881 tgcccaattt tctgcatcaa aaaattccaa cagttctgat gggattggtt gaaatggcgt
    50941 atgcaaaaat tccggtaatt catctaaatc acgttcttga agcaaaaaaa tattttcaga
    51001 atgataaaac tgataatgag ctatggcttg attatctgtc actagtttct tttgaaaata
    51061 catagattca aacgttcgca aagtcactcc ttgctgccct tcccgcaaca gttctagaat
    51121 ccccctcgat tccccggttt ttttcagata ttcttcataa gtaaggtatc caggttgttc
    51181 atttcctcga agcggaagaa taaagtaatt ggtcgtaaca cctctttttg taaattcctg
    51241 cttatatttt tctgcccgtt cttttctgcc gttatttgtt gcaccaaaaa atagatccgt
    51301 ctttggttcg ctagaagaaa aatccatatg tttcgaataa aaggaactat tatgttttag
    51361 tccaagttct ttcgcttcta aaaaatcaaa atgataaatc tcatcgattg ccggttcatc
    51421 ttgtgcctgc ttcagcatcg cctcgtgttc tgctttaaag tggttccaaa aaaataacac
    51481 gatttttgca ttggtaaatg gacgcacgcg tcgagccatc tccatatcaa atccataatc
    51541 taaaaaaacg atcagctgat aatttgccaa ctgttgtcga gctgatttaa tgaattccga
    51601 ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggaa aaaaataaag
    51661 tgatttattt ttcgcaatta gcaatacgtc gtttttgttc atctgttccg cctcactttc
    51721 tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa aacgcttcct
    51781 attattttta taaaaattta acatttctat gaaaatacga tgaaataaat cattcttcca
    51841 ttaaaatatg cccaaaagtt ttctgaatag ttgtaattat ctgaaaattt gttatcatta
    51901 gtcattatta ttattactat ttattcttac tatttattct tactatgcat tttgattgtg
    51961 aatttaaaag aaagaggtta tttatggaag ataaaaaatt gaaaaaacaa cttagctccc
    52021 gacatatcac catgctagct ttaggtggag cgattggtgc aggtctattt aaaggaagtg
    52081 gcgaagcgat tggaatcgcc ggcccttctg tattgatcgc ttttttgatt ggcggcataa
    52141 ttttatatat cgtgatgaaa ggtctgggaa aaattgtttt aagtggtggt gatacgcacc
    52201 acggattatc tggattgatt cgtccatatc taggtgctca tgctgccgat tttaccgatt
    52261 gggtctactg gtccatgtgg attatcaata tcattgctga agctgttgca gctgcttcct
    52321 ttttacagtt gtggttcccc cacatcccag cttgggtatt tgtttttatg ttggctgttt
    52381 taactactat catcaatctt tattcggtac gattattcgc agagacagaa tattggctgg
    52441 cctttgctaa aatcagtgtc attatcctat tgatcatttt tgctgcttat cttgtcggtc
    52501 agcaaatgtt gggtactggt gttttcccta cccttcaaca attaactgat catggtggtt
    52561 ttactcccca cggtatgaag ggaatcatca gttctttgct tgtagttatc tattcttacg
    52621 gcggttctga gttgattgct atcacagtaa gcgaagcaga tgatcctaaa cgtgcgattc
    52681 ctaaagcaat caaaggcgtc atgggaagaa tcatttcctt ttacattatt cctttatttt
    52741 tgcttttgat tatttttcct tggaatactt tagcaggcac gaacgttagt ccttttgtga
    52801 tggtatttga aaaaatgaat attccatttg cggcagatat cgtcaatttc gtgattgttc
    52861 ttgctctgtt ctcgtctatc aattcaggtg tatatgcttc ttcgagaatt ttgttcttcc
    52921 gcttgaaaga tcggaaaggt tcctctagaa aactagctgt tttaaacaag catcaagtac
    52981 cgcaacgtgc tgtgctgttt tgtaccagtg ttctttatct aggtgtggct ctttcttatt
    53041 ttgtcggtga caaattattt ggctatctgg caggttctct ttcctataca gtattattga
    53101 tctggatcat tatcagtgct gcgtcctttg tactgacact aaggaaagga acgttatggg
    53161 acaaaggaat cagctttttt gcattagcag tcttatggtt gattttcctt ggtatcctat
    53221 tcacgaactc cattggggta actgtattga ccggtttgct ttacctgttc atttatttca
    53281 gttatcaaaa aaagaatgat gctttcgtct tgacgaatga atgaaaccaa tagagaattt
    53341 tattctattt atattgaata tctggagcta ttgaaaaaaa ggagctgcga caaaaattgt
    53401 tgcagttctt ttttccgaat aaacggtgtt caaaaaccat ctgtgcagat taattgattg
    53461 ttctccacgc tcatgacatg atagaagaaa ggaatgaaga aaaaggagtt tgttttaaat
    53521 gaaaaagtat tggaagtttt tgttgatgat tggtgtttcg acggtagtca tgtttggtat
    53581 gatgtatttg aatgtttatg cgcttgatca tatcttcttc agtcgcacac gtgtattcat
    53641 ggcgttgatg atgggtgcag tcatggcagt catcatgttg ttgttcatgt ggaaaatgta
    53701 tgaaaacaaa aagataaatg ctatgatttt agccgctagt cttcttgttt ttgccggttc
    53761 cttatttatg gtacgaagcc aaacaactgt caacgatacg gcgtggatga aggcgatgat
    53821 ccctcatcac tctattgcta tcttgacaag taaacgagct gatttaaaag atcctgaagt
    53881 aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa gaaatcgaca aaatgaaaaa
    53941 aatgatcaaa gaaatggaat gaccattatc catccaaata aaaaaagagg atgttccaga
    54001 agtgatcaac ttcaagaaat aagaagaagc tacttctgga acaccgtgaa tcagaggttg
    54061 tgaaaaagct gccctgcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt
    54121 tttcgtttgg ttaggcttat taccgcaaat gcaagctttt gaacaccgtt tattcgtaaa
    54181 agggagtgtg acaaaagtct tgtcacactc cctttttacg tttttattgt tctaattgcg
    54241 ctgtcttgct tactgcttct tgcggtttca caaatttctt tgagatcccc caacctgtaa
    54301 aggcactgac catcgaaaac acaggtgaaa gcaaactgaa aaacacgaat ggcaagtaag
    54361 tgagtgtagg aatgccaaat gtacttgcta cgaaacttcc ggcgattccc caaggcacta
    54421 agtagttgat tactgttcca ccatcttcta gtgttctccc taatacagtt ggatcaaatc
    54481 ctgctttttc gtaagtttct ttaaatgcat taccaggcag gataacagat agaaattgtt
    54541 ctccaataaa gatatttact ccgatgcttg tcatcaatgt tgtaaaaatc aattttgggg
    54601 tgttcgtcat ctttcttgat actcgttcca taacggttga gatcaaccca aattcgatca
    54661 acaaaccacc taatgacaat gttagaataa tcaatgacac agttggcatc atgctttcga
    54721 ttcctccacg gctaagtagt gaatcgattt gttggtttcc agtatttgaa acaaatcccg
    54781 attcgatgac ccctgccaac ttcgttaagg aagtttgtgg gttttgaata aagatcattc
    54841 ctactgctac agcaatattc aacaaaatcg tcaaaattgc cggtaccttt ttccatgcac
    54901 acaagagcat taaaaataga ggaatcagtg accagatcga aatagtaaag tttgcttcta
    54961 aaatatcagt cacttcacga atagatgaaa gacttgcggc tttacttgtg tgtcctaaaa
    55021 tcatgaataa aaagaaagaa acaacaaatg ccggaacagt tgaccacatc atatgcttga
    55081 tgtgtttgaa caaatcggca tcaacaatag ctgctgctaa atttgttgat tcggataatg
    55141 gagacatctt gtctccaaaa acggcacctg aaacaatggc tccaacaaca agggctggat
    55201 ttagtcctag tgttgtccca ataccgaaaa atgcaatccc aatcgttgac ataacagtaa
    55261 aggcactccc tacagcgcta cctacgattg cacaaacaac aaatacagaa gggacgaacc
    55321 actggacact aatcaattta aatccaatga ccatgagggt tggaataatt cctgcttgta
    55381 tccatacagc aatcaatgaa ccgactaaaa taaaaataaa gattggaatg atccccggtt
    55441 tgatgccatt gatgatccct tcgtgcagcc aatctgtcgg gtattttttt atcactgcgt
    55501 acaacatgac cattccgata gctgccaaaa tcgtgatatt aggtgaaacc tttaatccga
    55561 caacgccaat cgcaatgcct aaaacaatga ccatcaatac aagtgttgct tctaaaaaac
    55621 ttggtgttcc ttttttttcc atgataatct ctcctttttt agttgttctt atgttctaaa
    55681 aaagttaaga tatcggatta ctccgccaca attcatccgc gattcctcct ttataaaaat
    55741 aaaaagacct gctttcaact aaggacgaat gactccgcgg taccacctta attaaaaaca
    55801 gatctgtttt tcactttcaa acgattattc aaatgtatgg actttccatg taattcgtca
    55861 attaggcttg atcggttcac agcgaccacc gactttctga acaaaacacc taaccactac
    55921 tgcgtgaaaa gttgacttgt atgaagttta ccttccataa gatcatttgt caatgaaaaa
    55981 gaaaatattt tttcattttt ctgaaaatta tttttataac tttcgcgcac aactttatct
    56041 ttttactaga aagtagcgcc ctcttgtttt tttcttgcta ctatttcttg atgttcttgt
    56101 ttgatttttt ctaatagctt gggttctctc aataaatcta gagctgtatt ggcaagtaat
    56161 tccgctgcaa tcgcaataga agcaagtcct ttttcacttt tagcagcagc tttgaattct
    56221 tctgtatgtc cggcaatata tgtctcagag attgctacag taggctgtat cgttggaata
    56281 acttggctaa catttcctac atcagaagaa cctagacttg ttctttgttc cgtttctatt
    56341 tcttcatcag gaacccctgc ttcttttgca tgttggaaga ataagtcatc gaaagaaggc
    56401 gttacgatga catcatcgac tgcattttgg aacaatccaa actcaaaagt cgtacccgtg
    56461 cttaatgcag cacctcgtac gatattttcc actttttcat atactttgtc taatgtttgt
    56521 cgatttcctg cacgtaaata gaaacgtcca gcagcgtagt ctggtatgac attggccgcc
    56581 acgcctccat ctgtaatgat tccatggata ttgacatctt ttggcaattg caagcgtaga
    56641 gcgttgattc cgttaaacac ttggatcaag gcttccaaag catttactcc tttttcaggt
    56701 gctgctgctg catgagaagc gacaccgtaa aatttgatat cgactggatc atttgctaaa
    56761 gattctgtcg ttttcccata acgaaaagct ggatggacac ataatgctgc atcgacatcc
    56821 tcaaaaaaac cttcccgaac gaagcttccc ttagctgatc cattttcgcc tccttcttcc
    56881 cccggagtac catacacacg aatttctccg ccaatctcat caatgaattg tttcactatg
    56941 ctggctgcta atacggagta agtaccaaat aaattatgtc cgcatgcatg accaatacct
    57001 ggtaaagcat caaattctgc caaaaaggca attgtcggac ctggtttatt ggaaatataa
    57061 cgtgcatcaa agcctgttcg gtgcccagct acttcttttt ctacccagaa cccttctttt
    57121 tccaattgtt tgattaaagc tgctgaggaa taaaattcat aattacttac ttcaggatgg
    57181 tcgtggatag ctagtgcaag tgcttgatat tctggtaaac ggtattgaat cagatccttc
    57241 aattgatttg ttgagagtgt ggttgtcata aaaatttcct cctatgctag tcgttttttc
    57301 ttattttctc ccgaatgttt cccacgcagg tacgcttgaa ccttttgatg ttttttcgat
    57361 tactttggct gtttcttctg tttgataggc atctactatt ttttgatacg ttttattgtc
    57421 ttcatcttct tttcttgcaa cgatgatatt gacataagga cgtgcatgct catcgactgg
    57481 ttctaagaag attgcatcag aagcgggatt aaatccagca tctacagcta caccgctatt
    57541 gatgactgaa atatctacat ctgacaaagc acgtgctgtt tgggaagcat ctaactcaga
    57601 aatgtctaga ttttttttat tctcggtaat atcagaaacg gtaggcgctt ggccttttgc
    57661 cttgtccact ttgatcaatc cagcagtttg caataacaga agagctctgc cgccattcgt
    57721 gacatcatta gggattgcaa cttttgctcc atctttgata tcagccacat cttttaacgt
    57781 attggaataa attcccaacg gtgcattaac cgtattccca atcgatacta aatcagttcc
    57841 gtgttcttca ttgtaattgt ctaaaaagaa ttgatgttgg aaggaattca aatcgatcga
    57901 tccatctgct aaagccgcat ttggctgatt gtaatctgta aatttcacat attccaaatg
    57961 gatgttttcc tttttcaaac ggtctttcac atcgtcccat acatctgtat cttccccaat
    58021 gatccctagt tttacggtcg tttctttttt ttcagttgta gctgcttttc cttggctgca
    58081 tcctgccaac caaataattg ctacaaatgt aatgactgca aaacgaataa tttttttcat
    58141 tttttctcct ccattttcaa tgtgtgactt ttttaatcag ttgattactg ataaattgac
    58201 tcaaaaaaac taagaccaga atcaataatg tagcgacaat cgttacatct gtttggaatc
    58261 gattatatcc tctcgatata gcaagattcc ctaatcctcc ggcacctact gctccagcca
    58321 tggtagtcag tccgatcaca ttgatgatcg tcaacgctga gacccgaatg ataccaggta
    58381 acccttctaa caaataaaca cgtaaaatga tgccgattgg gctcgtgccc atcgcttctg
    58441 ctgcttcgat caccccagga tcaacttcta gcagtgcatt ctcgatttga cgggcaaaaa
    58501 acgggacaac gccaaaaatc aaagggacta aagctgcttt ggcaccaatc gtcgttccaa
    58561 ctaagaaacg agtagtcaaa gctaaaagag aaagcaagat aatgaaaggg atggaacgaa
    58621 tgatattgat caacttatca agtatttcga acagcagcct gttagctagt aatccatttg
    58681 gacgtgtcac tactaacgtc acacctaaga tgattccgat gatcccacca atgaatgccg
    58741 tccaaaaaga catatatagt gtttgctttg tcgcttcgat gaattcatcc ggaatcaaat
    58801 agacattcgg caaatatgtt ttaaaaaatt cagtcatttt agtttcctcc ttcgatgact
    58861 ttgatacggt tatctaattc atttttttgt aaaatagtta cttttacacc agatttaatc
    58921 aaataatcca atgctttttg ccgctgtttg gtttctccag ctaaagtaac gatcaaactt
    58981 cctaagggtg tttcttgtaa tagctctaca ttaccgtata agatattcgc tgttacttgg
    59041 tacttgctgt aaagatgagc gattagaggt tcgttcgtct ggttgccgat ataacttaat
    59101 tccaccaacc atttatttgc aatctgatcg gcaaaagcag catgggcact gattgtttcc
    59161 aatgcttggt caaggtgtgt cgctgtccga ataaaatctt tagtcaattc ttcttcagga
    59221 tgactgaaaa tctggatgct gctacctttt tcaatgattt tccccgcttc cataaccgct
    59281 accttgttgc agatttcttt gacaacttgc atctcatgag tgatcaggac gattgttaga
    59341 cctagctgac gatttagttt ttttaacagt gcaaggatct gaagggtcgt tttcggatca
    59401 agtgcactag ttgcttcatc gcataacaac actttaggtt catttgccag agctcgtgcg
    59461 attgccactc gttgtttttg cccccccgac agttgtttag gatacgcaga tgctttttct
    59521 tccagaccga caagttccag tagttcattc actttttgac gacgttcctg ttttgatttt
    59581 ccagaatact ttagagaaaa atctacgttg tcaaaaatcg ttcgactttc cattagattg
    59641 aaatgctgaa agatcatacc gatggccttt ctttcttttc gcaattcttt tggagagagc
    59701 tggcttaaat tcgtctgatt gacgatgact tctcctgctg ttggtttctg caacaaattc
    59761 atcacgcgga ctaacgtact ctttcctgca cctgagtaac cgacgatccc ataaacatcc
    59821 cctttttcaa tcgttaaatc tacagaatct actgcctgta tctctttttc tttttgtttg
    59881 aaacatacag aaatatctcg caattcaatc attaacattc ctccttgtct ataaaaaaag
    59941 catcctcaag aaaatgaaaa tagtcatttt cttgaggacg cctttttgcg tgttaccacc
    60001 tcatgttcaa gctgtgttca cacacaactc ctcttcaagt accatcatac tctagcaaga
    60061 tatcggtcgc agccgtcacc gccttacatc tatgctcggg atgaattgct cgatggccat
    60121 tttccaatag tcgttccttg cttcttctca gctaccgaag ctctctgtca aaggttcttc
    60181 tatcgtactt accatttcaa tgcaaataat attcaaaaaa aaaatccttt tactaacata
    60241 aactatgtca gtaaaaggac gacgtatcgt ggtaccacct tttttcggaa gtttccttcc
    60301 ctcaagccca acatctcaaa aagagagatt ggcgttcgtt ttaacggtcg aacatttccg
    60361 aatttgccta cttatcgaca aattattctc caaggccatt ttcagctttt cttacttgac
    60421 tcttcacacc agccagagcc tctctgcaaa gtttccaagc ttactttcct tctcaacgaa
    60481 atcaatattt tgttgttggg aataatctac aggaggaaaa ctgaaaagtc aagcatgaaa
    60541 tccaaattat tttttctata tttatttttc tgttttttta tctaacattt caataacagt
    60601 tcgaagatct tttccttccg caagaaggtt caatttttcc ttctcttcga cgctataatt
    60661 tcgaacaaaa ctggaatcgg gaatcaaaac aacgcctagt cctgcacgtg aagcggctgt
    60721 cgctcctact aaagaatctt ccactgcaaa agctgaaaga gcttctaatc ctgttcgttc
    60781 caaagctgtc agataaggga ctggtgaagg cttgttctcc gatacttcat ctccgaacgt
    60841 aaacgtatca aagtaatcgg ctaattgaaa gtgatccaag atatctgttg ctcttttcgt
    60901 aacagttgtc gtagcaatcc ctagtttcat atgacgttct tttaagtctg tcaatatttc
    60961 ttttgcatag ggtttcaatt ggatagctcc acttgtcagc tgttgataaa aatattcttc
    61021 acgctttgct cggacttcct gcactttttc aggactgcct gctgcctgca ctaaaatatc
    61081 aaaactttgt ttccagtttt ttccgcccca agcgtcaacg atttctttcg ggatatgaat
    61141 atcaaattgc tggaatcccc aaagccaacc atcccgatac gttctttccg tatccactaa
    61201 caaaccatct aaatcaaata ttgcaccttt catctataca ttaatccttt ccaaaacaat
    61261 acacctagtt tagcatgact gatccaaaat gaacagaagc atagagccca gccgcatcac
    61321 ttataagagt tcttggaatc gacggatata gattttcttc acagctgtca caagcgccag
    61381 ataagcgacg actgttataa aaaaccataa ccagtaattg ccagggagcg gcatcaagcc
    61441 taactcagca ccaaatcctg tgaatggaag gactgaaccg acaatgattc ctaatgtagt
    61501 gataaccagc atggcaaatg ttgcattgct ttggataaat ggtattttcg gtgtacgcaa
    61561 tgcatggagt actaatgttt gtgtccacag ggattcgaca aaccatcctg catggaataa
    61621 tgcgataaaa atcacttgtt gtgagctatc caatgtatgg aaacttccac caactacagc
    61681 aggacaaata acaaaataca tcaacaagta agtagtgata tcaaaaatcg aacttgttgg
    61741 accaaaataa accatgaatt ttccaatact agatgcattc catttctttg gctcatccaa
    61801 atattcttta tccatccgat cccatggtaa ggaaatgcaa gaaacgtcat agatcagatt
    61861 caaaaataga agttgcaaag gcaacattgg taaaaatggt aagaacgtac tggcaatcag
    61921 tacggaaaac atattgccaa aattagaact tgctgttgcc ttgatatatt tcatgatgtt
    61981 tccaaaagtt tctcttccag aaagaaggcc tctttccaaa atcgtcaaat ctttttctaa
    62041 aaggatcacg tctgctgatt cttttgcgat atccacagct gtatccacgg aaataccgat
    62101 atcagccact ttcattgctg gagcatcatt gataccatct cctaaaaatc cgacagtatg
    62161 cccattttta cgtaaagctg tcgtcaatcg tgctttttgc tgaggattca attttacgaa
    62221 tacttcgtgt tcttcagcta cttgcgctaa ttcatgatca ttcatttccg agattttctc
    62281 accagtaatc aattcattga tttctaggcc tacttgacgg cacacagacc gtgtgactaa
    62341 ttcattgtcc ccagtcagaa cttttacagc tacaccatgt tcttttaaag ctttcaacgc
    62401 ttgttttgtt gtttcttttg gtggatcaag gaaagcaaga taaccaatca gaaccatgtc
    62461 tgactcatct ttgactgaaa attctccaac aacgcttgga ttggtctttt gtgcgacacc
    62521 aatcactcgt aagccgtctt cattaagtga ttgtactttt gcaagcaccg cttcttttcg
    62581 ttcttttgtt aatggtgcga cagtaccacc aagatcaata aatttagaaa tgttcaacat
    62641 ttcttcaatc gcgccttttg tgatcatctg tgttttgcca gccggatcag cgactacaac
    62701 actcatacgg cgacgttcaa aatcaaaagg aatttcatct actttttgat aattgattgt
    62761 atctgtatct aaagtttctg ctgcttcatc aatgatagcc acatctaaca aatttttcag
    62821 ccctgtttga tagtagctgt ttagataagc atggcgcaaa acacgatcat cttcatttcc
    62881 ttcacaatcc atatgatatt caagaatgat tttgtcttgt gtcaatgttc ccgttttatc
    62941 cgtacataac acatcgatcg caccaaaatt ctggatagca tttaaatttt tgatgactgt
    63001 tccttttttg gccatcacac ttgctccttt gaccaaattc gtcgtaacga tcattggaag
    63061 catttcaggc gttaaaccaa ctgccacaga taatccaaaa agaaaagctt ctaaccaatc
    63121 tccttttgta agcccattga taacgataac tgctggagcc atcaatgaca taaatttgat
    63181 cagcagcatg gatgttttgt ggatgcctaa ttcaaaactt gattggatgg gtttttcaga
    63241 tagcgtttct gctacatgcc caaatagtgt gtcatttccg acagaaacaa tgattccttc
    63301 cgcgcttcca ctgatgacat tgcttcccat aaagacaaga ttttcataac ttgtttcact
    63361 ttgctgatcg tcttcacata ctttcgcatg cttttctact ggatagcttt cccctgtcaa
    63421 tgctgcttga gaaatgaata gatcctttgc ggacaataaa cgcatatccg ctggtatcat
    63481 atctcctgct gaaagccgaa caagatctcc acagacgatt tcttccatag gaatctctgt
    63541 atactcgcca tttcgtttga cagctgccgt tactttcaca agtgatttca gtttttcagc
    63601 tgcttgatta gaacgcacag actggatcaa cgtcatcgtc ccactgacga taaccatagt
    63661 gaagataatg gctacaccaa agagatcttt atctcctgga gcggctaata cgtattccgt
    63721 aatgaatgaa ataattccta atgcaatcaa cactaatgta aagggtgtga tgtatgcttt
    63781 caatatttct ataatgaatg gtgttttttt gccatatgaa atcgcatttt caccataagt
    63841 ttctcttgaa cgctcgattt gttcttctga caatccattt ggtgatgcat ggaacaattc
    63901 aaataattct tgcttcgttt ttccagcaaa agctgcatag attttttgtg tatctttctt
    63961 tttttccatc tcgatctctc ctttgtgtcc aaaagacagc tgttctcgct gtttttggtc
    64021 ttttgctatt catcggatcg atagagaaaa tgctgaaagg ggctgagaac agatagataa
    64081 tagccctggc ctttcctcta aatccttgtt tttcgaatga ctgtcatcca tttgttctca
    64141 tctcctttac taagtaattt taattcatga cttccaatca tttttcgaat agttattttc
    64201 ccaactcgct cacctccctt tttgtttttt cacctatggg atttgcggca acaaaaaaac
    64261 ctctcgccaa atactcagcg aaaggtttga aaaagcaaat caaaaaaagc ttctcagatc
    64321 tcatcgttga gttttgacac tgtacaacgt aaaaagcaga tatctgcttt tagctacctt
    64381 atgaaaagcc ttatctcgac aattcctgtt ctacccattg gcgtctctcg acgtttttgg
    64441 gcagtagcct atgtttgtat aggagcctca cctaacaaat cctattcggt tttccctcgt
    64501 taatataaca actaaaaaag aggatgtcaa atcaattttc catgtttttc atactttttt
    64561 aaaatacacg cgttatattt cctgtctcac gcagttttat ttgtctcatt ttccacctcc
    64621 cgctataata gaattatagt aggaaacgag gtgctgttat ggaacgaaca cttgttatta
    64681 tcaagccaga cggcgtaaga cgccatttgg tcggaagcat catccaacgt tttgaagcaa
    64741 agggactggc cattgctgaa atgaaatttg caacgatgac acctgaacta gcaaaagaac
    64801 attatcagca cttgactgaa cgtagttttt ttgatgagct tatcgattac atgacttctg
    64861 gtccggtcgt ttatcttgtg ctggtcggcg aggaagtgat cgatatcgtc cgtaagatgg
    64921 tcggagcaac aaaaggggcg gatgccgttc caggtacgat ccgaggagat tatgcactgc
    64981 cggggactga aaacatcatc catgcttctg attcccgtga tgctgcaaca aaagagatcg
    65041 cccgcttttt cccgccattc gatacaaaca aaccgtctga aaaagcggcg aagtgataca
    65101 aatcctaaac tgttggtcta actgctgggt ttagcaatgg ccagcagttt tttctctaaa
    65161 gaattccttt gattcttccc taatgtatat tcgatcactg cgaaaaactc ttggtgattt
    65221 tccgattttt cttcccctaa agcagcagaa atcagtcgtt ggtaataatc cgccatcaat
    65281 gtttcataaa tcactgtact tctttcttta tgcgctaaac tccgtttcat atcttcattc
    65341 acgctacgtg ccttttttaa gcgaccattt tctatataaa acaaagtcaa gttcgtcaaa
    65401 aaagtaaaga gatactgctc gtgattattc agttgcgcga tccgtcgcat tctttttttg
    65461 attttcaaat aattattctc aataaaacga tcactgaata tccaaggaaa atctgctaga
    65521 atagatactt caaattgccc atactctggt acttgcatca aatattcttc aatgatacga
    65581 cattcataac gcaaaaattc aaattccgtc tcagatactt ctgttttttt ggacatgtgc
    65641 agattcaaaa ccaaatagta cagatcttct gttttcttat agtgcctgcg gttattagca
    65701 atttctttgt aaggatcaaa gctgtcttct tgaatccgat ctagcattcg tctttttttc
    65761 tttaattttt ttggctcatc aagctgattt cgtatccatt catattcggc aagtgtcatg
    65821 ttcattcgct ctaaaaatga tttcaatgtt ccaaacgata cttttttcac tgaatttgtc
    65881 tcgatcccga ctagcgaagt tttagaacag attccttccg ctaattgggt ctgtgtcagc
    65941 tttttgttga ctcgaagctt cttaatcaat tctccatcat acataatagt tctccttttt
    66001 taattgaaat taacccttgg cttttccaga atgcttgcca ataagaggaa tgattatttc
    66061 aatttaaatt ttccgcattc aataaataga tacgttaaaa ataaaaaata cacgaaaaaa
    66121 ataaaaaaat gtttttctca taaaaaatac caaccttcaa gcatgagact gctgtctaat
    66181 ccctgaaggt tggtttgatt gtttattgat cattttttta ctgctccgca ttggttcctg
    66241 ttactggata taggatagtc gttgttttat tgccgttttt tgaaaagaca ctggtcgaat
    66301 gttcaccttt ttgcttttcg attgcttcat tacgttccgc ttcgttttta tagtcatatt
    66361 ttttcggatc aatcgtctca aatccgtttg gcacataaaa ccgcaagaga ttttcctgat
    66421 tcagcttatc tgaaagactt aacgcagtct tgactttttc ctgatctttg gagatttcct
    66481 cgtcgattcc tgctgctttc gtatcgatga tttctcctgt ctgattattg taagcttttc
    66541 ctcctaggac ggtgtatttg ggcgtcacga agttcccatt tcgaaaagca actacttgat
    66601 cgtgctgaga agataataag tctgtaccaa aatgaagata atctttatca tcgattccca
    66661 atagatgtag taatgtcggc aaaacatcga tttctccacc atattcatga ttgactgtcc
    66721 ctttagtgta accaggcata tggatcatca atggcactcg ttgcatctgt gcgttgtcaa
    66781 attcatccca tgtatctgga tctttaccaa gagcacttgc taaatcttta ttatctgtat
    66841 tcgagatccc aaaatgatcc ccgtaaatca caaagattga attttggtaa ataccagatt
    66901 ttttcaaata cgtaaaaaat tgttccaatg attgatccag ataatgggcc gttcgaacat
    66961 aattatctac tgtgctattg ccagtattca aagatggaaa gtcgaaattc ttgtcatccg
    67021 tataataagg cgtgtggttg gttacggaaa gaaatttcgt gtaaaacggc tgttgtagat
    67081 gttcaagata ttgggcagat tctctgaata aatctttatc taatatccca taacctaacg
    67141 tttcatcgga ttcatcaaaa taagatcgat caaagaaatt gtcataccct aaatttttat
    67201 atacatgatc tctattccag aaactagcta cattcccatg gaaaacagca cttgtatagc
    67261 cttctgtttg accaagggtc gctggtgctg cttgaaagac attatctgac ccaagttctg
    67321 tgaataatga gccttgaggt aaaccaaatg tgccagtttc caacatgttt tcagcatcac
    67381 ttgtctttcc ctgtcctact tcatgaaaga aattgtcaaa actgatcgtt gcttgattct
    67441 ggaaaatact atttaaaaac ggcgtgactt cttgtccgtc taccttcata ttgatcaaaa
    67501 actgttgaaa actttctaaa tgaaggacaa tcacattttt tcctttagca atcccaaatg
    67561 tttcaggatt tggctctgca taatgtttct ttgtatagtc taaaacttca tcaattccat
    67621 tactgcttgc atgcgcgcga acactgctag tcattcctgt tttgattccg tcataaatag
    67681 tgtacgcatc gattcctaga tatttgacaa tataggaacg gtcaaatgtc cgctgtagca
    67741 attggggacg gttggcttcg ctcaacgcta aatttcctga aaaaatcaat cctgaaagac
    67801 aggtaacagc gatagccatc ggtttgctga cagggttgct tttgataggt attttcttga
    67861 ccttcaacca aaccagtagc ccaatgaaca cagcgatatc caaccaatag atgacatcat
    67921 ggggtttcat caaagaaaag gagcttcctg acaacccttg tgacactttc gaaaagccta
    67981 atacagattt gactgtaatg aaatctgtga attcccgata aaaaataata ttcaagtaca
    68041 acaaaagcgt atttaaaata tcaatgatca aaagaatggc taacgctggc tttggctttt
    68101 taatatacaa agaaaggcca aaaaacaata aggtagtcgc aatcgggttg atccacaaga
    68161 taaagtactg gatcgtacct tccacaccta atgaaaaatc tgcatagtag gcaatgatcg
    68221 tcttcagcca gaaaaagaaa acagctgtca aaacgattgc taaacgattt tggcacaatt
    68281 ttttaagttg tgtcacgtat atcacttctc tctattcaca ttttttctta ttttgctcct
    68341 gaaaaatgaa aatttcctaa aaaagttgtt atcttaagat ttttttattc tttctaaaga
    68401 ttattccatt catttttcac agcataaaat cgtttcggtg taagcggtta aaatagtaag
    68461 gagataaaaa tcttgtggtg accgatttac tctatagtat tcaaaagtag tttctcggta
    68521 ttcgtacaaa aaaaacatac taagattaga ggggaacatc attgacgaat gatgttctcc
    68581 tctattttct atttttagat tatagtgaag agtgaaagaa agttcgaaca aagccttgag
    68641 gactacgagt ccctatcaaa aaccgaactt ctttactgtc attttagaat caggtttacg
    68701 tatgttgggg ccatgagccc gtgtatagga aattggaatc gaaatgaagc agtaaagttc
    68761 tttcaacact taaaaaggag gaaaacaaat gccttacatt cttgcattag atgtttcaat
    68821 gggacacagc tacagtgtcc tatacaaaga cgacacctgc ttatttgaag acgagatcga
    68881 acacactcaa agaggatttc atgcattact cgaagagatc cagcacttgc ctgaacgtcc
    68941 attgattgtt tttgaagcaa cgggtattta ttccagacca gtcgaaaagt tctttcaaga
    69001 tcatcacttt tcttactgtt tgttaaaccc gttagaagcg aaaaaacaga tggaagaaaa
    69061 caccttacgc agttggaaaa cagataaatc ggatgcccat cggctcgcac agactcatct
    69121 taaaaatcaa cgtcaaccaa aagaagtaca aagcaatttt tacttagaga tgcgcgattt
    69181 agcccgtttt tatcaagaaa ttgagaagaa aatcactcga ctgcgcatgg atttacacaa
    69241 ctgccttcag ttgacttttc cagagcttga acagttcttt tcaaacaggc tgacccctta
    69301 cgcattgaca ttgatccgtc tttttcccca tccagatttt gtattggcat ctactcggac
    69361 aaaaataaaa aataaactga tcaacgaaac acgggaaaaa atctccgcga atcgtgccga
    69421 gcaaaaggca gatcaaatca tccactatgc acagtgtgcc tatccagctg ttgaaaaaga
    69481 cagtattcac tgtcaaaaaa cgatctatta cgccgaactt ctccaagatt tactcgaaca
    69541 aaaaaaagca ctggcgacac aaatgatcaa aaaagccgaa gcctctcctt gctttttgtt
    69601 gtaccaaacg ttccctggga ttggcgcact cacagccgct ttattgctcg gtgaattagg
    69661 agatatcaca cgatttaaaa cccataaaca attgaatgcc tttgtcggaa tcgacatccg
    69721 acggtatcat tcaggtaagt atactggcca agaccgcatc aataagcgtg gtaatcccaa
    69781 agcacggaaa attattttct tcaccattcg aaatatgatt cgccagcaac gcgcagctcc
    69841 taatcatatt gtcgattatt actacaaatt aaaaaagcaa cctatcccta agaaagataa
    69901 ggttgccaca gttgcttgta tgaacaagct tctgaaatgt atgcacgcta tggttagggc
    69961 acatacagag tacgactatg cgtacgcggt ctcagtggac cattaactaa accacttaca
    70021 gtataccact tctttgccca gatcgaaaat tttttacgaa cggggcttta tttagtgtgc
    70081 cttgattttg tcgctttcgt ttacgagcta tttttcttgc accttttact tgactaatcg
    70141 taggaaagag gctgtgacaa aattcttgtc gcagctccct atttcttgat gcaggacagc
    70201 tttttcacaa cctctccact aaacggtgtt caatcagctg accttcggta ttagctcaaa
    70261 aaacggcaaa atatgaggag ctattttccc gtttttttct ctaatactca ggtcaaaccg
    70321 ctgatttcac aacctctgat tcacggtgtt ccagaagtag cttcttcgga aataagccga
    70381 aattttacaa aaacttgaga agcacttttc gtaaatttct tcttatttct tgaagctgac
    70441 cacttctgtc acaacttctg tttttcattg tccataggtt tgatattgtc cttctgatga
    70501 ctcattcgta atgattgagg cttgattttc ttcatttccc gttatcgttt gtccactgcc
    70561 agaatctgaa gggcttagtt ctgtcgactc gtttttttga gaagaagcag ttgttttctt
    70621 gacttgttcg ttggtctttt tccattccgc tagtcgatcg gccaccactt ttttgtccac
    70681 tttttcagaa taagctgtaa ccacttttcc atttttcaaa cgtgctacct tgtcgccatc
    70741 atgagtcaaa agcaaaacga ccaaatgttt gtctttgaat tgatattgtc tgagagaaat
    70801 cgcactaagt acgtcatcga tattgataaa ttgattttct tcttttcctt ttggcaaata
    70861 gatgtccaat tcaccttttt cgtaattggc agttaattta tattcctccc accaataagt
    70921 tgagaattct tttttgaaat tatctactga ttctttgctt gctacgattt tttctgtttc
    70981 tttctgagca agatctgctg tcatttttcc tgaaaatgct gcggataaaa taagcagtaa
    71041 gaaaaaaccg aataaccaaa agagaagaaa cggtcttttt ttcctcatgc cttcctccac
    71101 tcctatttaa ttttgactat tctattatat acaaaaaatg aaatagaaag acaaaaaagt
    71161 tgaaaaagat cgtaaataat tgaacttctt tttcctgaag cggtaagaac gggaattttc
    71221 atacattctc tcttggctat tctttaagct tcaaatccat ttctgtctga tacatctttg
    71281 aaccaatgac ccgatttctt gatcgttctt tttccatctt gtgctaaatc tactgaaata
    71341 aaaccgtaac gattcttata ggcattggtc cacgaccagt tatccatgca tgtccacata
    71401 tgataaccta aacaattggc accttcttga atggcttgat ggacatattt cagatgagat
    71461 tgaacaaatt cgatccgata atcatcgtgg atcataccat cttccttgat aaatcgtgct
    71521 tcattttcta cacccatgcc gttttcagaa ataaaacagt cgatattccc ataatgatct
    71581 cgtacattga tcaaaatatc atagattcct ttttcataga tctcccatcc gcgataagga
    71641 ttcattttac gaccaggcat ttcataattg tcgaagtaat cctcaggcat aggaccgttt
    71701 ttagtatcga ttggtgtttc ttttgctttt attcggcgtg gttggtaata attaattccc
    71761 aaaagatcta ctgtgttttc tttaatgatc gtcaaatcat ctggatgaat ctccggcatt
    71821 agatcgagtt cttttattct ctcaatcaat tcactgggaa actcgccttt tacagctgga
    71881 tcaaggaatg aacgattgaa gaacgcatct gctattcgtg ctgctttaac atcttcggga
    71941 tttgattcgt ctcttggata actcggtgtc aaatttaaga tgattccgat cacaccgctt
    72001 tgatccattt catggtaagc tttgatggct aatgctgaag ccaacgcttc atgataacca
    72061 acttggattg cctttttcat atctgattct tccggataat gccatcgata caaatagcct
    72121 gcctcgactg gaacgattgg ttcattatgg gtaaaccatt tttttacttt cgagccaaac
    72181 aattgaaaac aactttttgc gtatttttca taggcttcaa ccgtttctcg gttcaaccat
    72241 ccgccttttt cttgcagata catcggcata tcgaaatgat acagattaac aaagggttcg
    72301 ataccgtttt caatcaactc atcaaaatag tgatgataga aacgaacggc ttcttcgttg
    72361 attgttccag attcatcagg tatcaaacgg ctccattgga tagaggttcg aaatgaatta
    72421 tgcccagtct gtttcatcaa ctgtacatcc tcttgatact tcgtataaac ttgcgaggtt
    72481 ttttcaggtc ctacttgatc aaagaacttt tgcggttcgt ttttgtacca ataatcaaaa
    72541 atattctctc cttttccatc gcctgaaatt cttccttctg tttgtggacc acttgctgca
    72601 gagccccacc aaaaattttt tggaaattga tatcttttca tcaaagcacc tctttttctt
    72661 ttttagttga ttccatgtat gatattttcc atcacaatca ctttttcatt ttcttcctct
    72721 tcttttttga ttcgtttcaa cagctgttct gctgctcgtt ccccgatttt atctgttggt
    72781 ggcgtgatcg tgatttttgg aaggatactc agatcgtttt tatctaaata atcatagtga
    72841 ctgaacgcaa tatccttccc gattctgaca ccttcttgat tgaaacattc gatggcacca
    72901 atcgacatgt tgtaattgct gacaaaaata gcagttgccc ctttttctaa taatgctttt
    72961 gccccgtaat agccgctttt tctagaatag tctcctgtaa agatctctaa atggatagac
    73021 ggatcctttt gtaccgcttc tttggctcca gctaaacgtt ctcttgctac gtaatctgtt
    73081 tgagcggcag caataaatcc tattgtttta tgtccttgtc cgccatgatg agcaatcact
    73141 tttgctacac tttctcgatc attcactact attgtatcaa tacctgctgg cccacccgga
    73201 caattcaatg cgacaactgg gatactcatt tttgtcagca tttcaaatcc agaccactct
    73261 tcttctgata agaagacgat caatccatcg atcgcatgct tcatcaggta ttcgattttt
    73321 tgttcgattt tttctggctg atcggcaaac ccgcttaata ataagctata cccattttct
    73381 tcagctactc tttcgatccc tgaaacaacc tccgaaccaa atcggttaga tatcccgttt
    73441 atcaacagtc caatagagaa acttcgatta ttcttcaatc ctttagcaat gatgttttct
    73501 ttatagtcca acgctgaaat cgcttctgat attttttcca tattcttttc cttcagctgc
    73561 tgaccgttta aatagcggga aactgttccc actgaaacac cagccatctt agccacgtca
    73621 cggatcgatg ccatacattt tcactcctgc cagttacatt tgatttcttt ttcttgttgt
    73681 tcgaccatga tgaccgcttt ctttgcataa tgccgcttgt catcaatatc catataacta
    73741 aagtcttcag aagtgagttc aaaaacaagc agtctttcct cttgagaccg caattcatct
    73801 ttacgaaaag ctttcaataa tttcgttctt tggatgacaa cgccgtcaat taatcggaca
    73861 tagacaagag tacttgtttt acctgaaaca cttcctgtat ttttaacttt aattgatatt
    73921 attactttgt caatatccgt tttgacttcc atctgactgt attcaaatgt tgtatagctt
    73981 agaccatgtc caaaaggata aaatggactt ccgcttaaat cataataatt ctcttgttta
    74041 gaagcagcac gctgatagta ataaacaggt agttgccgac tatttctagg ataagacaaa
    74101 ctcaatcgtc cgcttgggtt atttcttcca gaaatcatct gcgcaatggc ttttccaccc
    74161 tcctgtccag gaaaccatgc tgttgcaatg gcattcgtaa atgactcgat tttcatcaaa
    74221 tcgtagggtc ttccttgcac aagtatcgtg atgattggct tgcctagttg atacaattgt
    74281 tcaatcagct gttcttggta tccacctaaa gaaagagaag caacatctac attttctcca
    74341 gaatccatgt tgatcccttt cgatgaaact gccccgttac gaagaaattc catatcaaag
    74401 ttccgcgcac ttgatccacc tagaactgca atgatcacat ctgagttttg agcaagggtc
    74461 accgcttcta ataatttttc ttcctgatcc tcctgatttc ttacttcaca tccttgtgca
    74521 taagaaacct ttgattgggg aaactctgtt tttatctctt gtactatctt ttttttcaaa
    74581 cggtcttctg tttgtggtgc agagtagtct cccaacaagt ggtaaacatc gtgagcattt
    74641 ggacctataa cagcaatgtt ttttacttgt tgatccaacg gcaatatacc gtcatttttt
    74701 agtaaagtga tactttcttt tgctgtctct aaatttaagt gctctgattc ctccatgaga
    74761 tgatcccaat actcctttgg atcgtttgca aatggctgtt caaataaacc taataaaaat
    74821 ttgacagata aaactcggcg aactgcttga tctagtagct tttcatctac gacttgatta
    74881 tcaatggctt ctgcaatctt ggtataggtc tcatcccaca aactgagatc gattcctgcc
    74941 gctaatgcat atgctgcagc agtcttttta tctgtaaaaa catcggataa acgatccaaa
    75001 gccacaccgt ctgccataac gattccttga taaccgatct cttttcttaa tgtttgctca
    75061 aaaagcgctc gattgatatg acaaggtatc ccgtcgatgt cattataagc agccatgaca
    75121 cctactgcat ttcttgtact cttcaataat ggtccgtaaa tatcggagaa ctctctgcgt
    75181 ccaatcgtta ctgtgcctga attatgaccg cctaaagctt ctccttgtgc gatacagtgc
    75241 tttaacacaa caccgatctg atccttcttc ttattgattt cctctactgt ctgatcaaga
    75301 aaagaaactt tttctgaaat aatctctcct tggaaccctc gaacgaccgc ttcagaaaaa
    75361 cgagctgaga gaatcggatc ttccccaaag cactcttctg tccttcccca acgaggatct
    75421 tttgccagat ccaaagtcga caccagcgct aaatgaacac ctttagcagc cagttctttt
    75481 gccatcaatc gactcgattg ttctaataat gctacattga aagtatttcc acggccgata
    75541 ttcgttggat aagaaatccc atccaagcct tgatgaccat gtggacattc ttctacaagc
    75601 aaaactggaa ttttccatcg cgaatgctgt agtacatatt cttggactag gttagccacc
    75661 ttccagcttt cttttgcagg aatcccattc ttataatctg ttttagacca cggatcagag
    75721 cgaaacagac cgtataaggc acccagaccg cctccccatt taacatgttc ttttaacttt
    75781 tctgttaatt cgatcttgtc tccgtttttt tcataacatt cccaaccata caaatgctgg
    75841 tttacctgtc ctactttttc agcaatattc atcctcgaaa gtaaatcttc tactctttgg
    75901 cggatggtta actttgggtt ttgatacggg taatcgtaca ccttaatatc ctccttctgc
    75961 caattttgcc ttattagcgg ctaatacaaa tggcaggaat aaaaatacat ctagaatgat
    76021 aagtattact gttaagactg ctgctcgtat atcgcctcct gtagcaataa aagattgtat
    76081 aaccggtgga gtgatccaag gtgcagcagc aactgtttta ctgatgattc caagacttgt
    76141 ggctgcgtag gcaataacca agttaataga tggtataata gcgcatggaa tcatatatat
    76201 tggattcatg actactggta atccaaacat aattggttcc gtaatattaa ataaacaagt
    76261 agtcagacca aatttagcga ttgatcgctg ttcttgacgt ttagaagccc acaagatagc
    76321 aataactaac ggtaaaatac acccgccacc tccgatcaat ccaaatgcat tggtaaaggg
    76381 ctccgtaata atattaggaa tttcttttcc tactgaaaaa gcatccatgt tttcctgaat
    76441 agactgtaac aaaggtgctt gccgtatagg agccaaaata ctcgaaccat gcataccaaa
    76501 cacccataag aaatcagaga aaaactgtaa aaataagatg cctgggacgg ataaaacaaa
    76561 agctttcaat ggtgtctgaa tcgctaattc aattaattgc ggaacattca aaccagtcgt
    76621 atggtttaaa gtaaaaacga caactgaaaa aaataaaatg acaactattt ctggtactaa
    76681 agaattaaat gacgcagcta ctgccggtgg aactccttct ggcatcctga ttttaaaact
    76741 cttcattttc gctaatttga taaataattc tgtactgaca agggcagcaa tcatcgccgt
    76801 tgctaagcca gacgaagaag tcagactttg taagaaaacc cctgtagctt ctgctgtatt
    76861 tccgtccatc aaagtgactg tacttgataa agacatcaaa ataaacatta cggctgtact
    76921 tagaattcct gcatgaacag gactaaatga tggatcatca atcacgcctg tttggatata
    76981 gtgggttgcc aaattccagc cgatattgta acacacgata atagctaaaa tactcatcgt
    77041 accattattc acgattgaag caagttcagc caatggactt agatcaagta ttctttggac
    77101 tgttgcattt gaaaaaatca gagcattcag caaaaccatg acagaagcag ccattactaa
    77161 cggcatagta acgataaaac tgtctcgaat ggccagcaag tgtcgctggc tactcatctt
    77221 agatacgatt ggtagcattt tttcagatag tctgtctacg actttcatta tttcatctcc
    77281 tagattctaa aattgaaccg gttcattttt aatatagcac tttttttaaa gattgcaaca
    77341 cattttttta aaacgctttc taaaggtggt ggatttttta tgacttttga aaaattattc
    77401 tatttaatgg tattttgtga attttttctc ttccatccgt ttgaagtttt actttccaat
    77461 ggtataattt ctttatccta tcaaatgggg gaagtatttt gaagaaatta atcgtactat
    77521 ttattttgtc tgcgggactt ttgacattga ctggctgtga tttgtttggc ggctctgacc
    77581 aatcctcgga cgaaacaacc gattcttctg tcttaaaagc ggaaaaagca gatatcacca
    77641 acagtgtata tacgctaaaa gtacgtaaag atatggattt atccgcggga cagttgacat
    77701 ttacagataa aaaaatggaa tggacacgca cgtatcagac acaaggagct gattcttctg
    77761 aatcatctga agaaaaaaca gtgctgacag atatcaagat tactaccaaa gatgacgaat
    77821 atattatttc aggaaaagaa aataaaaaag aagcaacagt cacatttacc aaaatcggta
    77881 attaccgttt gaaggataag gatggcaaca tctacagtct atagtgccat ctgttggatc
    77941 ttcttttgtt tctccccttc ttttctgcta tagtaaagaa gcaagatgac tgtaaaggag
    78001 acgaaatgga tgatcgatag tcctgattat tgtacatgta ataacaacaa aaatatccat
    78061 acagaattta atgatcaagg aagtttgagt atatgcgaca attgccacaa actagtcaaa
    78121 gattctttta aatcgtttga atcttatcct gtcaaaaatt atgtaaaaag caaaagaaga
    78181 taagcaaaat caagcaaaac tcactatggc tgatttttat gaacttcaaa ctcagcttct
    78241 tcctttgatc aagcttcaaa aactggaatc agtaaaacaa acagtggatc atttcagttc
    78301 acaaatcaaa gagtaacgtt ttatttattt cacatctttg aatataaaat aaccaaacga
    78361 tagaaagact tattctacat cacgtagtaa tttttctttc ccgtcgtttg gttttttact
    78421 tttagcctaa ttcttgacct ttcgcatgct gattatctgt aaaatatttt ccagcaggct
    78481 ttccttccaa ttcagctaat ttttcttgga tctgacgaat cacctttttt gcatcttctg
    78541 gattttctac aaaatcgaat gtatccccat ccacttgtat tttaggacat acatcaaaat
    78601 cctcatacca ttgattatag cgggtgttca gtgtttcgta ataggtgtat aacgtttcat
    78661 cgctttctag ctgttcatag tcacgcccgc gttttttgat acgttctagc atcgtgtcca
    78721 aagaaacttt gatatggacc atcaaatcag gacgtttttt tggagcaaca tcatctaact
    78781 cgtttagcat cgtatctaat aagctatcgt actgctgtac ttcgatatcc gttacccgac
    78841 ctaaatcagc atttagatgg aacaacagac tatcttcgta gatggagcgg tctagcacat
    78901 tgtcatcgtt cactaaagca tttttcattg ctaaaaatcg cttattcaaa aagaagattt
    78961 gcaataaaaa tgcgtactgc tcaggatttg aataaaaaag cggcaatact tcgttatctt
    79021 cgacattttc ataaaatggt ttagaatcaa tctcttttgc aagcatatcc gtcaaactgc
    79081 tcttacctgc accaatcgtt cctgaaatca aaatcactga catacaaccc ttcctcttct
    79141 ataagtactc gcctacgtaa gtttcaacag gcaatttttc aaaatcatat tcacgtacag
    79201 cttggtctga ttttaaatac gtgtgaagac tttgtgtaat catcaagttc gtaggtaccg
    79261 tctcttcttg tacgatgatc atcggttttt tcaacatagt tgccacacct aattcgtaag
    79321 ccgttcctgg atcgattgtt tgatgctcaa agtcaaggat agccaaaaca aattctgcat
    79381 ttgtcaactc ttccatatct ttttcgtata cttcctgcgc ccatccagcg ctgaatagtt
    79441 cataattgct ttcttggtga tgacgtggag agtagaaact tgtgattgtt ttgttttcct
    79501 ccaacgcttt ttcgatacga cttactcgat cgatttgctc ctctgaaaaa aatggtcctg
    79561 ctaaatatat ttgactcatt attttgcctc cttcaataat aactgatttt taatagatac
    79621 tgctcgtaat tcattgacca gttccaatgg attgttggaa aagatcactt gatccaacca
    79681 ttttttatcc ataaaatcct cttttgcaaa agtttctaat aattgttgca agccgctgta
    79741 ataaccgcca atattcaata ttccgattgg cttatcaggt gtcaggccga tcgctatcca
    79801 agaaagaagt tgcgaaaatt cctccaatgt acctagacct ccagggaaaa cgaggacagc
    79861 atctcctttt tcaaataata atacctttct ttcatcaatt gtctctgtag gaataaatgt
    79921 agtaacatct ttcccaggca gctcagtttc aaaaaggcca tttggataga tacctgtgac
    79981 ttttccgtta ttttccagta ctgttttact aatcactccc ataatcccgg agtatgaacc
    80041 accgtaaacc agttcgattt cttcttgtgc catatatttt cctaacgttt gcgcaataaa
    80101 tttgtaactt tctttattgc caaaacggga accgcaaaaa acagtaagtt tcataaaaaa
    80161 acactcccta actcaaaagt ctatcttcat tctacacgtg tttttttaaa ataaaagact
    80221 agattttgta gttttttagt ggtatataga tcaaacacca ctatatatag tatttgcaaa
    80281 aaaataattt ttcagctatt ttacagtaac caaaaaaata aataactagt aaatataaaa
    80341 ttgaaacgac ttacttatag aagaaattgg gatttcgtcc tgcatgtcaa taacacgcat
    80401 tatttaaata gtcacacact tgtttctcct attaatgaaa aatccatttt attctcgtgt
    80461 tatgtttcac caacgcctga tgtttctcta aataaaaaag ccgacgaacc aaataatcga
    80521 aatttgatca ttttggttca tcagcttgtt cccaaacctt tgtattaacg ataaatagct
    80581 ggattcgtaa aactatcact tggatcatca ctgctggaag aaaattcggt gataaccgcg
    80641 cctgttgcac ctgcctggaa ccagtgttta atattttgtt cgatcgtgta ctgatctcca
    80701 gcgtgaagta tgatttcttt ttttgccgta tagtaacgtt cttgcccctt tggaataata
    80761 caggaaagtt tttttgtttc ttttccttcg acataaagat atactaagcc ttttcgacaa
    80821 cgaaaggttt ccttttttcc ttcattctca tctcgaggtg ggtgccgatg ctctggacaa
    80881 gtttgataag gcaataatac cagttctttc ccacagtatc gctcattatt ttcataaaca
    80941 aataaattca atccttcagt ctcgattgct cctaatccaa aatctgtata ttcgattctt
    81001 tctaattctg tttctttcaa tactactcct gaatctagga acatttgttg tactcttttc
    81061 ttgtaatcca ttctgttcct cctaactgat tgtcaagtca tcccaagata tctcttcttc
    81121 ctctttttct tcttggatga cttctgcatt tgggtcaact ggttttttca ataataaaat
    81181 agttattccc gtagcaaaag aaccagctaa caatgcacca atgaatgccc atggtctggt
    81241 catcgtagcg actgcaaaca ttcccgatgc cggaatacgg ctatctgcgc cccaaaacat
    81301 gctcaatcca ccgccgactg ccccaccgac tcccgttgct atgacattgc gtgccaagtc
    81361 gttcaatacg atagggatac atccctctgt gatctgacag atccccatag gaaaagcggt
    81421 cttcaatgta tcgatttctt gacgattgaa tatattttta cgaatgattt tccctaagaa
    81481 ataagcgatc gtcaagccga atggggttac catcgaagct aaaatcaata cagtgattgg
    81541 ttcatagatt ccttctgaaa atcgtgccac acccccttat ccagaataaa tggtaatcca
    81601 gaaacagccg ggtatctgcg gtaataagcc taaccaaacg aaaaatatga gaagctaata
    81661 tgagaagcta tttttgtttg gctgttctta ttacttgatg cagggcagct ttttcacaac
    81721 ctcttttttt atttatgttt cataatcaat atggtaagga tgcagctccg tatggtagac
    81781 cgcctccgaa tattcaatca tttccccaat ttgcgtagac gatttccgta tccgtttgat
    81841 gccgatctgt gcgtctgttt tcagaatttc ttgttcttct tctgttaaaa atactgccga
    81901 gaaatcatca gaaaatgagt caatagtata tccttttttc gtcagcaaac gatacaaaga
    81961 ttctttgcca aattcttctc tgctgaattg catcatttct tgtggaagat aatgaacaaa
    82021 ataaatataa ggctgattgt tcaaataata taagcgctta aaacaaacag cttgctcacc
    82081 gaaaaaggaa aacaaaggat cttccttctt tatatggacc aattgcaaat ccagcgtttc
    82141 tttccggact tcatatcggg agttctccaa gatttgcgta aagcttgccg ctttggataa
    82201 tcgattgtaa ggacgattgc ttagaaccgt cgtcccacga ccacttttct ttgcgacata
    82261 ttcatctgat gccagcagtt cgattgcctt tcggacagtg attttgctga cttgaaacag
    82321 ttcttctaat tcactttctg tcggtaacat cgaaccgact ggatattttc ctgagaaaat
    82381 atcttcttta attttatctg cgacttccaa gtatagtaca cgtgtccttt tcataacgtc
    82441 accctttgct ttactattac taaaagaata gcacataaaa aataaaaaaa gaacacaaat
    82501 cacatgtgag aagtgtgttc tttttcattc agctgcttta attacaagtt tacgccattt
    82561 gaggcaatta cttctttata ccaatcaaaa gattttttct ttgatcgatt cagtgtccca
    82621 tttccttcat cgtctagatc cacatagata aagccgtaac gtttactcat ttctcctgtt
    82681 gatgcactga caagatccat acatccccaa ggcgtatagc ccatcaaatc tacaccgtct
    82741 gctactgcat tagccatcgc ttcgatatga cgacgtaaat aatcgatccg ataatcatca
    82801 ataatcgtgc catcctcttc catatgatca atggctccaa gaccgttttc aacaatgaat
    82861 aatggtttct ggtaacgatt gtataattca ttcaatgcga ttctcagccc ttctggatcg
    82921 atctgccagc cccattcact tgcctcaaga aacggatttt ttactcctcc cagcaagttc
    82981 ccttctgaag ttgctgcttg tggatcagtc tcattgattg ccgtagacat ataataacta
    83041 aatccgatgt agtccactgg atattctttt agcaactgga agtctttttc cgtttgctct
    83101 aatttttccg gatcgactcc atatttttta tgggtacgcg ctgtgtatgc agggtattct
    83161 ccacgtactt ggacatccgt gcagaaaaaa ttgaattctt ggttttggat cattgttgct
    83221 gcctgattga ctggattggc atcgatgctg taagttgttg catagataat catgcagccg
    83281 atttggatat ccgaacgcaa ttcgtggccg atttttacgg ctttgctact tgcaacgaat
    83341 tgattatgcc atgcttggaa aatattttgg tactctcctg ctccattgct ttttactaat
    83401 ccttgactta aagctggaaa gtgaaaggcc gaattgattt cattgaaagt catccaatat
    83461 ttgactttac tgctgtatct ctctaatact gtctgagcaa atcgttcata gaagtcgatc
    83521 agttttcgat ttttccagcc accgtattct tttgccaaat gaagaggcat ttcgtaatga
    83581 gaaatcgtga tcacaggttc aatatcatat tttaagcact catcaatcag ctgatcataa
    83641 aattctaatc ccgcttcgtt aggagtacct tcgtctccat ttggaaaaat tcgtgtccag
    83701 gcaatcgaaa aacgataaca tttgaatccc atttccgcaa acaaggcaag atcttctttg
    83761 aaacgatcgt aatgatcaat tcctcgatga ttcgggtaaa tatatttttc ttgatcaatc
    83821 gtccagtcaa attcttcact gccgataatc gcaaagcgct gcttacctcc aggcatagca
    83881 tctgcaacgg atagcccttt tccgtctcga tcataagccc cttctaattg attggcagca
    83941 gttgctccac cccataaaaa tttttctgaa aaacgtgatt gcatcctgat ttcctcctta
    84001 ttttacaatt gccagaacat cttcgtttgc aataatctct ttttggtcta attcaagtat
    84061 atcggcaaat gcatcagtat tcgtaatgac aactggagtg attacgctat agccagctgc
    84121 tctaattgct tcgatgtcaa aacgaatcaa taaatctcct gctttgacga gatccccttg
    84181 tttgactaag atctcaaagc cattcccatt taattctact gtatccatcc cgatatgaat
    84241 caaaatctcc gctccgtcag tcgttgtgat ccctacagca tgtccagttg gaaaaatagt
    84301 cgtgatctct ccgtctgcag gtgcaactag ttctcctact tctggatcaa tagcgatacc
    84361 ttttcccatc acgccagaag aaaaaacttg gtctggtact tcactcaatg gcagtacttt
    84421 acccgttaaa ggacttttta aggtatgccg tgctgaagta atcggttctc ctgcattcgc
    84481 atgtttgttt tctaattgat tgtcttgtgt ttgttcgtca aatcctaaaa tcaatgtacc
    84541 tacgaatgca agaacaaaag caattcctgt agctattact gctactgtca catttgactc
    84601 tacaccatcc accgtattaa tgaaagtcgg aatcgtcaat aagctgacca aactgctgga
    84661 gaaagctttt actccggaaa atccgataat agctccacca atacctccag aaatacaagc
    84721 ggcaatgaat ggtttcttca aaggtaatgt cacaccatac acagttggtt cagtgattcc
    84781 aaataacgaa gtcactgtag aagaaatccc taatgctcga agtttcgtat cttttgttcg
    84841 caatgctaca gctaaagcag ccccaccttg tgccaagatg gctggaagaa gcattggcat
    84901 caatacatca aacccatatt gttcgatatt caaaaacatg atcggtacga atccccaatg
    84961 catgccgaac atcacgaata cttgccataa acttcccatg attagacctg ctacgattgg
    85021 actaaatcca taaatcgagt tgaagagacc gcctaaagca ttaccggcaa ccgtcccgat
    85081 tggtcctaaa gcaagaaatg ttaatggaac catcactact aaaacgacaa gcggaactaa
    85141 aatcatttgc agaaattgcg gaatcacttt ttttacgaat ggctctaatt tactttgaac
    85201 ccatacggct agaatgattg gtatgacaga agaagtataa ccagatggtg aaagaatcac
    85261 tgggatccca aagaaactga tagcttctcc cgcaccagca actgctgtga tagctggata
    85321 cacaagtgcc atagcaattg ctactgctaa aaactggttg gctttgaact ttttagctgc
    85381 tgtaaaagca agcatgacag gtaagaaggt aaagacacca tctgctgcag caaacaatat
    85441 ttgataagta ccgctatcag ctgtcagcca gcccatcacc gttgccaatg ataagaatcc
    85501 tttcaatacc ccggcggctg ccatcgcccc tagaaaaggt gtaaatatac tagagataat
    85561 atcgatcaga cgattcaaaa tatttttgga ctcttctttt ccatcctccg aaactgcaag
    85621 accttcctca tccacgatcg attgataaac atctgctaca ttactgccaa tcactacttg
    85681 atactgaccg ccactttgta cgacttggat cacgtccggg tcttctttta acttttgcgt
    85741 atctgctaaa gattcatctt ttaatctgaa tcgcaaccgc gtagcacaat gtactaaact
    85801 tttgacattc ttttgtcctc ctaccgcttc ccatacacga cgaccaactg cttgattatc
    85861 cattgttttt tccttctttc ttttttattt aaaaaataaa aacccagatt gaaaaaaaca
    85921 gtcacaaaca ttcgtgaacc tattttttca atccaggttt tgcctgccat acagtaacaa
    85981 tccttatgat tggatgcttt ttttctcact tactactctt tgaatatgaa tcgttaaata
    86041 aatctgctct gcgatcccca tttcctgttt ttgcgtttgt cgaagatact cattgatccg
    86101 ttcgacacaa tgaaacgctt ttggatactt tccttgaact aatgcgtaga gaaattcatc
    86161 ttgctcgtca gatccttccc ctttgagata acgctgggca aaaaactgca gatgagtaat
    86221 gatacgctga taattcaaag attcttcatc gaacacgatg ccgaagaatt tactgatgat
    86281 actcagtata tctcttacca ttgtggtagc ggtcatcgtc acttgcatgt cttcgtttcc
    86341 ttgttggcta ttcacaaaat gaaaggcaat aaaaccggct tcatcctcag aaagttccac
    86401 acccgtcttt tgtttcacga acaaaagagc ctctttcgct gcttgatatt ctttcggata
    86461 aaattttttt gtttcaaata ataatggatt aggcagttgg atattttctt ttgctcgtgt
    86521 gatcgcataa tgaagatgat cggtcaatgt cagataaata ttaggcgaaa attcaacttt
    86581 tagtttatct tcagccagtt cgactacttg tttgacccat tcgatctcct ctgccggaat
    86641 atcagccatc agctgctgga attggttttt agcaaaagaa tctcttaaaa caaattcttt
    86701 ttggaccgct tctggcgaaa tctcgtcccc aatctttttt tgaaaagcta gtccttttcc
    86761 catacaaacg acttcttccc cgaatccatt tttcgaaatg acgacattgt tattcaaaat
    86821 tttatcaatt ttcattttgc ttcgccctct caagaaaaat aaaaaaacct agaattcgcc
    86881 tactgtttct tagctgaatt ctggttttgc ctgccgtaca gtaacaatcc atttcttatt
    86941 caaaatcatc ataccatcga atttttattt atgcaagcct tttcatataa aaaataaaaa
    87001 aactggaaca caagtctatc tagaaaacaa ccaaatcaga gatgcacctc tttgcgtttc
    87061 tctttttttc agcaaattgg tttttcctta tcgattcata tcctttggct gtttttaatc
    87121 tgtaacgact tatgtcccag tatcctaaaa tgtttttgtc tctagattga aagcagatct
    87181 catatcatct gtcgcatctt ttatcgatgt aacactttcc agttctcctt gtacaactag
    87241 tcgcgtgacg gcgtccatgt cgccacaagt aattagtgtg acaatttctt ttccctcgac
    87301 ttcattcagt aattgggtat ctgtaggagc aactttttct ttaaaaaatg ttttgtatgt
    87361 atacacattt tctaaatctg tcaaatagat cttggcaccc atctctaaat tttctagcgg
    87421 tgtaaacagc aattcagggt tcgttgcacg atggcttgct aaggcataat ttccttttcc
    87481 catttcttga tctggagata atgttcctgc tccgtaaagt agtgacgtat tatccaagcc
    87541 tttgaaaacc ggtaaattga ttgaaactga agggactgcg atactagcaa tcactggtag
    87601 atctgttcct ctcatttgcg acctcatgac cccttctgtc gtcattggtt ctaccgcatc
    87661 aaagtcaaaa ctgacatctt tatcgttatt ctttttcaga tcttcttttg ttacattcgc
    87721 tatagcatat ttatctcctg tctctctgac aaaataatcc ttgatctgtt cattgaaaat
    87781 caaagctagt cctactaata acaagaggaa caagaggata ttgactagcc atctttttgc
    87841 ttttcctttt tttttcattt tctctccctc cagactcatt ttactgtttt tcttttgctc
    87901 tttccaacag ccgaatgaca catttctaca actctgtgta tttttttcgt acacctgcgc
    87961 ttttttctac aggatatttc aaattatttt tgatcaattt ttcttcaatg atctcatcta
    88021 gatccaggtg aaggttgtct gccatcatgt atgaatagat caatacatct gccagttctt
    88081 ctttgattcg ttctaattgt gtttctttta cttcttccgg cgttttccat tgaaaaagtt
    88141 ctaacaattc agatgcttcc aaagaaatag agatagccaa atctttttcg ttatgaaatt
    88201 gtcgccaatt acgttcatct ctaaactggt tgactttttc catactattc atttgcttgc
    88261 cttcttccaa tcacttctta cttgattaat cataccattt ttttacataa aaaatagatg
    88321 atgcttttaa aaaaactcct gttttctttt atttaaaata aatgtcatag aaaatcaata
    88381 atttaggtag ccaccatgtg tatgcagtag cgtgtcttct tcccttgagg ctttttgttt
    88441 ttcgatacgt tcttcttccc aaacagacaa cgctactaga atctctctta atgattttcc
    88501 ttcttcggtg agcgagtact ctactttagg cgggacttgg ttataggctt ttcgatggat
    88561 actttcatct tgttctaact ctcttaactg ctgggtcagc attttttttg tgatcgtagg
    88621 gatttgcttg cttctccacg cctaaaataa gcgatattgc tcttcccttt atctggtgta
    88681 agcaaagcag ttagcatttt gacgatcgtg ggttccgctt ttcacttctt atctacttag
    88741 tatcatgaag tatactttcg tactaatact aagtagcgga agtgtcaact actttgttta
    88801 actacatagc aaaagaaaca gaagggtgac aacggtcatc acgcttctgt ttcttttgaa
    88861 tatgctgtgt tgttgtctct atctccgtgt agactttcct caaacttatc ctttgacagt
    88921 aagtcagtag gctgatttgc tctttctaga tgccagatac atttctacaa aaaaatagtg
    88981 attctgtttt gaaaattcga tttttagttc tgcattttcg taaacatcca acattttttt
    89041 cgcactgtat agacctaacc cgccattttt tttagaggtt tttcctcttt tgaccatctc
    89101 cgatagcgtc aaattcgttg gatttgcctt gttcgctata ctaaaacgtg atccctcttt
    89161 ttcttccagc agattgatcg taatcggact ctgatcggct acacgatgat cgaatgcgtt
    89221 agaaataaaa atagataaac agcgaataaa aacaaccaga tcaatcggga ttgttttcac
    89281 ttcgacagga ataactactt ggatcgggat tttctccgat tctgcttttt ctagaaattt
    89341 taaaaagaca cccctgacag ctggggatac aatatttgtt agctgttgta tttgcggttt
    89401 ttcaaaaata gctgcagaat gatcttctaa tatttctaaa aattccaacg tttctttttt
    89461 attttcctga agagtaattt ttaatgaaaa cagtagactt ttataatcat gtttgaattc
    89521 ctctagatga tggtaatatt cttcatttat ttgatacagt tgaagcgatt cctgatgttg
    89581 tctttctttt acttggaaac tgatcaacat aatggaaaaa aacaaagaaa aaaaactgat
    89641 gactatcgac aaaagagata gaagcagata attagatgag cggaagtgaa cacttcccaa
    89701 taccaagtaa atcacgcccc agcttaataa aatacaaata cctccaaaat agtgttcagt
    89761 tggataagta aaaagcaagt tgagaaatct cgtctgtttg tctatacttt ttactaatag
    89821 atagaaaata aatgcgataa gtagtgcaaa aaaacttgct gttccaagat gtctctttcc
    89881 taaataatcc atcagatcaa aaaccagaaa aaagctgagt ccagtgatag caaaaaaaag
    89941 attactgatc aaacagctga taaacaggtt tttcgtagta aaataaaaaa gaaacatcaa
    90001 taataagcca attaggtaag gaaagctcac tttagggata ggaagtaaaa gtgtcaccac
    90061 catcaaagct atacaaccaa agctgttttt tactagcagt tgtttcgaaa agaataaata
    90121 gatacctaaa tctaaaatca aagtaaaggt caaataaagt gtcactgttg cgaccatctt
    90181 attcctccat tgttttgttt caaaatgtac acatattttt ttaataggag cctgcatatg
    90241 aaaaagtatt ttatcataga agataaccag tttcacagcg aatggatcat cagagaattg
    90301 gtcaatcatc atatcgagaa tatcgtgcag ctcacttcta tctctcaaat caatcagttg
    90361 atccagttgg gcactattga taatcacgat gttttcttta tcgatatcga cttgaagcaa
    90421 tcactcacag gtattgatgt cgccgaacaa atacgtaaaa aaaatgcaca tagtaatatt
    90481 gtttttttca ccgcttatac gaattttgca gtagagatca tcaatcggaa aatccttccg
    90541 attggatata tcagaaaaga caggaattta tctgaacaaa ttcgtcaagt gctagaacag
    90601 atcaattgga aagaacagca gatcgtaaat gtcgataaaa tctttattga tcaaaaaggt
    90661 ggaagtctcg tcctgtatcc tcgggaaatt ttatatattg aaagtatcaa aggtttgaaa
    90721 aaccaagtta tggtcaaaac aactacagaa gataaattag tgaatacttc tttaaaacaa
    90781 ttgaagacag agttagaaaa gtatgcttac tttttacttt taaaatctta ctgtatcaac
    90841 ttatcgcaat tgcagaaaat tgattctgct cattatgtgt tagaattttt caacggtgac
    90901 tccttactcg ttggtcgaaa gatctttgaa aagacaaagg aacgtttcca tgacttccaa
    90961 aaaactgctt cgagttgatg aataagagct gagacgtaag catagcagat ttcagggagt
    91021 gggccttgaa tcggctccac tccctgaagt cgttgatatg attttacgct tcctcttctg
    91081 taggagcatc tgttaacgac catgtgaatg ttgaaacgta gttgtctaca gcataattgg
    91141 atacaggaac aaataattcc actgattgtc tcccttgttc cacattctct cctaatgaat
    91201 aaatccatgt gccaatccct tcatttgctc ctgcagttac tagatctact gcctgatctg
    91261 ctgggatttc gattccttca gcgccagttc cagaaacggt agatggcatt ccactgctat
    91321 tagttgcgct atttcgaatc acggcgtttt tgaatttcac ctttgaacca gttagtttat
    91381 tcgtatcaga tgttgtacca ataaattctg ttcgtttgac tgataatttc caaccggcat
    91441 aatttccgct tttatccgtt acttgtacga aattctgtac ttgtgtttgt gctccagtac
    91501 tgtccgttcc gctttgtaat gcagcatata ctgtttttgc tgtacctaac ttgattgttc
    91561 caaaatcaag atctggagct tggtcaatcg tcagtaatcc ggatgttccc tgatttggtt
    91621 cgtcaggtgt aaaaggagta tcaggatcat ttggatcaac aggaggaact ggaacaacgt
    91681 cttcaggatt tgcagcatct ggtttggtca cagaaatcgt tccttctgta gtggctgagc
    91741 tcttattctc agcaaaaaca ctttgtgtac ataataatag acttccagct agaacggtac
    91801 ttaatgcaat tgtttttttc atttcttttt ccttctttct tttaaaataa ataatccagc
    91861 aaataagcca ataagaatga ctccaacaag tatcagccat cgccagtcaa atgtttctgg
    91921 ctgatttttt aaatcaatat ccttttcatt caattggtcc gctgcatcat cagttatttc
    91981 aaaatcggta tggagattcc agcgtttttc tttccaagca cccttcacaa ccgcttgata
    92041 atgtcccgga gctaattttt ttccttctaa tgggacaagt aaatcaaaat ttgtattggg
    92101 tgctacatct attttttctt tttttattga aaggtaaggc tcttttgttt cttttttgta
    92161 gatttcaacc gatgtagtca agccgaacat ataagaagca tttggatttt gaagattcgc
    92221 cttaatcaca tttcttccat cgctttgatc cgcataagct ttttttaatt tcaaatctgg
    92281 ggaagactct ttttcgctat ttcaaatcaa gattgctttc acttgagaaa actgattgac
    92341 gacaccaact gtacttttgt tagaaacgtt ctcttttttt tcttgaaacg tgatccctcc
    92401 tgcgaccaca ccagccagct ctttttctgg cgctttcagt gaaagtgtct cagtgaccgc
    92461 actttgagga ggaacagtta tttctcccac tgtttttgct aagtttttca tatcataact
    92521 taatgtttca tcaaatttac tttttgattc tccatattcg atcacaccgt ttgaattcgt
    92581 cgtagcggag tggatcaatg tctcaactgt tactgattta tctgttcggt taaagaccgt
    92641 gtaggggatt tcctgtgttt tcccaggctc catccttaca tttatatatg ttttgttctt
    92701 atcaatctga ttagccggta attcaaaatc taccgcaaaa ttgaattcgg aggcatggac
    92761 aggtgtctgc caaaggatga atccaaggag aaggatcata agtaaagagc agttgtttcg
    92821 atgactttta ttccatattc gattcatcaa tcatcctcct tgatctttat tctgtcggcg
    92881 catctgctaa tgtctatgtt atctctgctg agtaattacc agctatctga ttttctttgg
    92941 ttaagacaag tttaaacgcc ctaccgttat ttaaatattc tgaataatct gcttgcacat
    93001 tcgttgaatt ttgtcctctg acaatttcca gttcttcttc attcactaat tgatactcct
    93061 ctcctttctt gaaatatagg acatcctgca tagaaaatcc tgcatcattt gtgaaaggct
    93121 gagataattt tatcgttaag atccacggat ttttcgtttc tcgctgatca taaatccata
    93181 atttttcctg tatatccatt tggccaaatg cttcttgtgt caaaccagtt gctttgatct
    93241 gaccaaaatc gagtttggaa ggggcaagaa atgagagacc accttctact acgatcttcg
    93301 tagcaggtgc aaaaggcggg gcatcatgat aaacaactgg cgtatcactt ggattgtgat
    93361 tcccaatttc atcgttggta cttggcttgt tctgcacacc cttagcagta gcttctcctg
    93421 cttggtcgtt taatacaatt tctaatacat ctcctgcaaa aagttgattt tctgggatag
    93481 aaagaacaaa ttcccctgta tcatcaatag ttgtttctga caaaacagct tgattttttt
    93541 tcaatgaaac caaggctcct gcttcacctg tacctgtgat cgtgcgagaa gctgcattga
    93601 tcaatgtggt acttaaatta gcaggttcag gaggaatcac atcgactgtc gttttggcag
    93661 agtccagttc tgtcacttct tggccacctc ttactttttt agcagcaaca acttgcactt
    93721 gatcctccgt ttttaatctc tcatcattgg gtacttgaac ttcgaacaag ccgccttgtt
    93781 cgccttctcc ccatgggtta tatcctgccc cttcattttc atctgtttgt ccttgcgtga
    93841 gagcagtaat ttctacagat gatttcgtcg tatctgcgta agtaatacgt aaagtcactt
    93901 ctacttcgcc tgtccaagca tctcttggct ttgcctcgac accttcggga acaacgacat
    93961 gcccatagat ttttttatct gcattcgttg gcactcttaa ggattcaatc atcgggagct
    94021 gattattggc actgattcga ttataatttt tcattccggc acctgtctga gcaaattcat
    94081 ttgttaaacc tgcactaggg cttgatacga aattgcctaa gtcggtccca ttgatcgata
    94141 agtctgtcat atcagaaaaa taatccgggt ctttttcaaa gttatcgaat gttgcgcctg
    94201 tttttcgcca taaagcgatt tctgaatggg cgatctttaa agaagaatct ccatttcctt
    94261 ggaataccca gccgcctatg ccattggtct cgcttccaat ccgtttgttt tggaaatcaa
    94321 aataggtagg agattctaag tccacattca acacatcccc actgatgatg ccggtcttat
    94381 cttcccctgt tttcccgacc atgactaaat aagttttttc acctgccgta aaatttaaat
    94441 caatatctcc agttgtatct acagttgctc cggaatctgc gcgtatatcg atatttgatt
    94501 tttctccagt gactgtaaaa tcagctgaac ctggactttc tagaaaacca gaagcgtcat
    94561 aaaattggat cgcttgattt ccttgattga gtctgttcac gtaagatttg ccatcacctg
    94621 tatttcttac cctcaatgag cctccattgg ttactgtcag cttttgcgta ccattctcaa
    94681 aacgaactgc gctggaacgc ccactctgct tatcgatgtc gataactgat ccggctcctt
    94741 ctacgatcaa agaaaggcct tttcctacaa aacgcatccc attgttcccg ggttgatcgt
    94801 catctccagc cattttgacc gagatttgtc caccacttga tacatggatt tgactagaat
    94861 ctccttgcaa gcgaaatccg cccgtagttg ctgtattgat ggtgattttc ccaccattcg
    94921 atacagtgac tttcgatcct gaacctctca tttggatgat tccgcgattg ttgtcattgg
    94981 taatattgat ctttgtattt accgaaaatt cgcttcccga accactgact tcaaacatga
    95041 cattaccagg tagcgtatcg actgcctctc cttcatcgct aacgccatct gcccgatcat
    95101 aatcaagaga gacctttgaa ccgcttaaga tatggattcc tggcgctgtt tttccactac
    95161 cagtctcatc tcctgaatct agaaaaccgt agaatttccc tagatacggt ccataaagtt
    95221 gactaccatt gatcacattc aattgatagc ttctacaaaa ataggcttta cccggagcgg
    95281 cattcagtcc ttccttcact ggttggacat ccaacacacc ggaaacacca tctaatgtga
    95341 cagttgcacg aggagcgtaa atcagtccta atttggaccc atcatacaaa tcaagtgtcc
    95401 cggtaaattt tatttctccc gtaaaatgat atctgcttcc ccaaaaatcg gtcatataag
    95461 caatgataat ggcatttcca tcgatatttg gtgtcaatcc acctggatgt gcagctgttc
    95521 ctttaaaatt ctggaactcg atcgactgag gtatctcatt gatataaata tggtgtgtgc
    95581 cgaaattgtg agcgaatcca ccaaaatcaa taacgatatc tcgtttgatc tctaattccc
    95641 tgtctaaagt caaaggctga atcagtcgga tcttagttac tgattcgtct gcgagaagct
    95701 gcgccagttc ttctgttgtg gcagcttcgc tgtacccatc cgctgaaaca gctcttgcca
    95761 gtgccgcttc actgctatca gacgtttgtt cagctacttc tgaggtctca cttgtcttat
    95821 tttcttcttg ccttttatta gtagaagcct cactttgaga ttctgtagtt tcagaagtaa
    95881 tcgattcttc tgaaaaagta gtcttgtcaa aactttgatt ctctgattgc gttgattcgt
    95941 ttgttttctc tacatcggca aaaacggatg tcaccatcaa gagagggaag acaaacaaaa
    96001 tcgacgcaat cagctttttt acacattgat tcattctcca ccatcctctc tgacgttttg
    96061 acaattttat tttatcgttt taatctattt ccaattgtac tttcatattt aaaatatgta
    96121 tttttatgga aaaatttttc tattaggtgt aaatttcttt tttgtacaaa aaaaaggctg
    96181 tgacaaaagt cttgtcacag tcccttatcc cgaacaagcg gtgttctccc gccaactcct
    96241 cggtcacaac ctccatttta acaataaacc tgtaaatgtg agggcaatac tttgattgtc
    96301 acaggtaatt tatccccttc atctccatca acattcgtcg tcaattcttc ctcatcagct
    96361 aatgacacaa ctaattgctc acaagtaagg taggctagat ttttcgtaga ttcatctact
    96421 ccttttaata ggtctggcaa tgttttaagc gtatcgatca aagaagaatc ttttaaataa
    96481 accaaatgga gcttaccatc atccactttt gcatttggaa gtaatgtttc aaaaccgccg
    96541 actgagttag ttagtcctat cagcaaggta ctgctctcaa tttcttcctt ctttccatca
    96601 acttctacgt gaaacgagta ggattgtgtg gaagctaact gtttgattcc tgacatgaaa
    96661 taagctagtt ttccaaattt cgtcttcttc tccgcatcta catcattgat tgattcaggg
    96721 atagagccaa ttgcgacgac attcataaaa tagtcgtcat ttatttttcc aatatccaat
    96781 ggcttgactg attcaatcga aaaatgattg atcgcttcct caggttctag cggtatccct
    96841 aatgctcgag ccaagtcatt caccgtaccc aatgggaaaa atccgaaatt tggccgatgt
    96901 tcttgttcag cgattccact gattccttcg ttgactgttc catctccgcc catgacaaac
    96961 acactatgat acccttccgt agccgcttcg cgagtaaagt tttttgcatc ccctgctttt
    97021 tttgtatgca agacgaccac ttcatcaaac accgattcca attttgctat cgctaattgc
    97081 tcaaattcct ttgcttgttc accgcctgaa ctggggttta ccactaataa aacttttttc
    97141 atcactttct ttcccttctg attgatttct ttaatataac gatagcataa agaaaaaaat
    97201 taggctgtga tgaagtactg ttcatgaaac attcatttat tttttgacaa gataaaacat
    97261 ccatactatg aaactgagga aaattaaaac aatgatatag gaaataaaag aaaatgatag
    97321 gcattgtata cctaataaag taaaaatctt aggtggcaga taaaaacaca aaaatagcaa
    97381 gagaatgtaa tagcgtttct tccacttttt gttatcagac cttatagcga tcagaggaat
    97441 accaaatgca taactgaaaa actcttgatg tactgttaat atgtcacggc cttgaataat
    97501 aaatgcgaca atccccgcta taattagtgt gcctaacacc aaaacggaaa attgatgaat
    97561 atattttata tacaaatgat ccattgtact tcctgccttt ttatttgtga gaaaaacaaa
    97621 tccgtatacg tgaataactt ctagaaataa aagaaatttg caaaaccact ccatttatct
    97681 acttctacgt gcattttttg atattgtttt ctttatttaa gattatacag aaaaatatct
    97741 agggtcgctg gattctaagt aaaaaaaatt tttaaaataa aaagaactaa atcaagtagt
    97801 aattaaacta ctgttgattt agttctaata aaaagattta gatgaacagt tttattgatt
    97861 taaatagttc atatttttta tttataccgc ttatttacta cttgctgtac tagaatctga
    97921 agtgaatgta ctcaaaacat tttcaaagtc tttgtctttg atttttacat tagcgtcttt
    97981 caatacttcg ccaattactt tattctggaa ggtactgtca cttaattttg tgtctgttgc
    98041 aatttctttc aattcttttt tgtatttatc catgtcgttg cctttgtttt ggtttttcac
    98101 cattttaacc acataatatt ccgtagtata agttgaagca ttggtagaag tgatcacatc
    98161 agaaacttga ccatctttca acttaaaggc tgcttctttg acttctgccg gaaccgttgt
    98221 agtagtggaa tcaaatttca cttttccgcc atcttctttt gtcgtagtat cagttgactt
    98281 gtctttggct aatttagaga aatcgtctcc cttatcagca gctttcttca catcttttgc
    98341 atcatcttca cttgctactt ggataatttg agcttccact tctggatgga aagattccca
    98401 agccgttttt aaatcttcat cggtcaattt gatatgtttc tttagacctt cttgaaaggc
    98461 tagactttgt ttgatactgt ctttgaatgt ttgttctgtg taacctgcag atttcaattg
    98521 tgaatcaaat gaatcaccca attgtttctt tgtttgatca tattgagaat cgatttcttt
    98581 ggttgatacg tcatctccat atttatcttc aaatacttga taaacgatca actgttgcac
    98641 agcggaatct gctccttgca tttttttcaa gctatccatt acttcttgtt cgctgattgt
    98701 accgccttta taactaacgg ctgttttttc accggcattc gtacagcctg acaataagcc
    98761 agtgcctagt acgatggcaa gtgctaaaaa tctttttttc atttatcaat tctcctactt
    98821 ttcgtttttt gcgaattgct aaaaacaaag gtgttcaaga tgaaaatcat caagaacacc
    98881 tttactaatc tgctcgatac gtcctgaata gatataaggc tacacaatcc agaaagttcg
    98941 ctcagcagaa taaatgtcaa gtagcacatc acaccttaat atactacttc ttttgttacc
    99001 gtttttcaag tctatcttgc gttccgtatt taacgtaact aattcttaga acaaatgtta
    99061 tcgattttgt ttttcttctt ccgctgctgg gatattcaaa tcagacactt ttatgttcga
    99121 ttttccagac atttttttgt tgtttttatt atgatatatt tttatacaac taaaaatgaa
    99181 tatattaaaa ctaccaaaaa taacttttaa cgctgtttca ttatatgttt tttctatttt
    99241 atataaactt ttggatactt aataccaacc ggttggattg taccaacatg agaatgtgtc
    99301 agttctattt cctgtcgcac ttgcataggc ccatactcct tttgttgcat ttgctgatcg
    99361 aatagagttt ccaactttcg cttttgcacc gtgacgatta tagacacgac tatagtggtt
    99421 agaatgacct gtttttctcg tacaagttgt atcactataa taaacccatc ttccagagct
    99481 atgattaatt gctgttctag aaatttcccg gtctactggt aaatttgact taataatatt
    99541 acctttagca tcaaaatgaa tctctcctgg ctttaaagat ttgtaataga tttcaccgtg
    99601 aactgaattt gcttccactt cagatgacat agcgtttcct ccaacaaaac ctatccctag
    99661 tgcacaaata ataactgctt ttttcataaa acgacatcct ttcctttttg atttttaaag
    99721 catgatcttc ttgtttccta ttttggtcag atatggatca tgcgtgacta cgataactaa
    99781 cgtaccttgg gaagcttgtt gcgataagat ttcaactact ttatccttat ttcttttatc
    99841 caatgaccca gtcggttcat cggctaaaat gattttgcta ttcttcaata agactctcgc
    99901 acaggcgatt ctttgcttct caccaccaga taatgtgtag ataggcgtac ttagcttctt
    99961 gtttatattt acttttttca atgcctcata ctttgctgtt cgcttatctg aaacatttaa
   100021 attcgaaaac ttcaatccaa tatctaaatt aaaatcgatt gtttcatatt caatcaaccc
   100081 aaaatcttga aaaagaaaag atatggtatt tctttttatc aagcgcttct ctctcccagt
   100141 taattgtttc gtctcttgat cgaacaaata gaattgtcct gaactgaact gatcattcaa
   100201 tcctaatata ttcaataacg tacttttacc tgctccactt tctccaacaa cagtcacaag
   100261 atccccagca tccgcagtga aacgatccaa atgaagaatc gttttgtttc gaatgatttt
   100321 tttcgcacct tttatcacta tcaacggctc caattgttaa cctccattca tcagcaataa
   100381 ctgacttttc ttttctgtag acaaaataaa aacaactgaa agaataaaat caatgaagct
   100441 aaagaaaaaa atattaaaaa gatatttagg ctctctttct ataagaaaaa ggaagattac
   100501 acttgtcgtt ccccatttga tgaggttcat catgaagaaa tctttatacg tttcaaagag
   100561 cgaataacca cttgaacgaa agatagcgat tttccgacaa ttgtagacaa aatatatgca
   100621 taacatgaca tagctcaaca agaagaaaga aaagaaagtg agtaaaaata tctgtatcaa
   100681 gtaatgaaat gatctgctta acactttgac agctatctct tcagtctgag tatacggaaa
   100741 tgataactgc aagttatcac gtaactgact ttcttcaatc agctcaaata acctttcttt
   100801 cgacaaagag cctatcttga tctttaacgg tggatagatt tctccgctga gaatatttct
   100861 ttccaaatac atactgtttt tcaatgtcag tatttcaaca accggataat gttcgatcca
   100921 ggggatagaa ggaataaatg tataaatggg ttgattgttt tttataaata gaaatttcgg
   100981 ttctttttca ctgatttctt gataatattc tgttatttgc gccaggtcat tttttaaatc
   101041 tgtatgtcta tatcgttcag ggatcaatat aacagggtgc gtttcttttt cggttatttg
   101101 gatcaacctt ccagagacat caatcaaagg aaatttctta agataattca gatttatttt
   101161 tatccctctg ccaaactgtt cattttcttc attcaagtaa tctgatacat tgactaatat
   101221 acttccgttt ttattcaaag tttcatataa ttcctgatct gctgctgatg caaaacgatt
   101281 ggaataaata aaatccacgt ggtttttccc gatggttact ggataaaata catgatagtc
   101341 ctcaccagcg atttcttttg gtggttcatg ggaaacagct aactgtgaag aagaaaaaac
   101401 gatagttgct aattcgacat tggcaacggt caaaagaaga gaaaacaaca atccaaatgt
   101461 aaaaagaatg tattggatat agttgaacgc ttctcttaac gaagcgattt tttcaactaa
   101521 taaaagagaa atcaaagaga taccatatat tccgctgaac aagaaaacgt acatctggaa
   101581 caaataaaga aattgctcct tataatacat aaaaataaaa ccaccaatga ttcctgcaat
   101641 tatgaatgta ataatgattt tctttttccc caataaacac cacaatactt gatgtaatga
   101701 aaagtcatta ttcaacaata aactgatttc tttattcatc aataagaaaa aggatgataa
   101761 caaaaggaat aaaagaatca atccaatagt taaatacact cgataattat atacgtctgt
   101821 atttgaaata accgaatacg aatagctatt ttcaaagtct tcaaaaatat tagtggcata
   101881 atcagtgtga aatgcttgat tgaatcgatc agtgaatagt gaaatgaccc gttcataggc
   101941 ttctttttct ctcgtcatca agaaaaattc accttcaaaa tcttttattt tgatcgattc
   102001 ttcaatggac gttacgcgaa aggaggtatt agaatagggt accttctcgt gagaagattt
   102061 gacatcccct tgataataca atgtcatttc aagctttggc tgtatcttga agttccaaga
   102121 tgtatctact tcattggatt catttactgt ttttttcata taagaaagct cagtttctga
   102181 tgagacattt tcaaatacca tgattagctg ctcatattct tcttgcccaa taaaatgttg
   102241 tggcaatcga atcggagtat aagtatctgt ttgatggaaa aattcatttg tccgatagag
   102301 tacctgttta tttatactaa ccaaggcaaa accactcatg ataccggtca ctatgattgt
   102361 gagtaaaact gtgaactttt ttttgattat caccactctc ccttacttaa atgattcttt
   102421 gataaaatca taccgttttt catcaaaaaa acgtatctat caagtgtata ggtcatgtag
   102481 aataaatcta tatactgtag aatcgaaagg agaagaagat gaacaccgga gaaaaaatac
   102541 gaacttaccg cagaaattgt aagcttacgc aaaaagaatt aggcgaaaaa attggtgttt
   102601 ccgataaaac gatttctagt tgggaaaatt ctcgtacaat gccagacttt gaaatgctgt
   102661 cacttttaca tcatacttta ggtctgccga ttgattttcc ttctattgaa tcagctaccg
   102721 aatcaataga accaacattg ccagatgaac ctcttgttaa caagaaatct tattcatcaa
   102781 aacaaccctt ctcactcaaa aagacactat ctttctttct gctcgtcttt tgtttggtct
   102841 atctttttct tatcaattat caatacaaaa atgaaacgat cgatcgattc aatccttttt
   102901 tgaaaccaca aatcggctat accacactcc ctgtaggata cgtggacaat tccatcgaac
   102961 gttgggtatg taatgatatc tttggccatg gtacatggct gtcttttcac gctggagagg
   103021 tagaaaaaga gaaaaaccat gctgtaatca aacacaaagg aaaacacgtc atgaacgttc
   103081 gcctgctcac ttgggaagcc attccctcag attaccaaga aatcctcagc caagactatg
   103141 acgacgcctt aaaaagaagc cctcaggagc cagataggca ttaagtagga gatgaacgta
   103201 gagatagaaa aaatcaatgc acatgatctt atccaaaaaa caggggtaca aattaattga
   103261 aaaagccttg agatgaaagt tcctaaataa aaaagttcag aatgataggt atttctggct
   103321 tatagtataa tatttacgcc acagccttcc aatgattctt tttggaatca taatcaagga
   103381 tggacattca caaaaatatt ttgctcattc gatccgaata gattaacgac caatctagta
   103441 agtaatatag attggtacgt agtcaagctg cacataattt agatcaattt tagttctttt
   103501 ttatatttct atgatctgct catttacttt tatatgatgg cttacagtaa cataaataaa
   103561 tatgataatc gctggtagtt tatccaagag tactcaataa aatacaaaat ggattgttca
   103621 agcgcctatc ataatgataa aatgaaacaa ataaacgata caaggaggaa gagactttga
   103681 gtaatggcag aacaattttt tcattgtaaa aattgttcag ttgttaatct atgggatttc
   103741 ggtagcatgg gcattttttt atagaggaat cacaccaacc tggccgtttt atctcttgtt
   103801 agtgggaata ttagtaggaa attttgcccc tcttcactat cgtacgaact tttttccaac
   103861 tgattctggc ctttttctta aaacaaagac gcgaaaatta gaaaacatta ttgaaatgac
   103921 aattacctgt ataatcattt tcttagttat attcctaaac gtcttctaat aagaatctct
   103981 taaaattagc ttgttacagt tagttaccta gtttatataa cacagttatc ttatcttcct
   104041 tatcaacatc ttttgccaac tctttgtctt aaattcgaaa catataagca gagctacgaa
   104101 caccaagact gtttcggctc tgttttttgc atcaaattca ctcttatata gctgataatc
   104161 tcggctcttt aaaagaaatt attttcgctt cattttatta taaaaaggct aggaaccttg
   104221 atgttatcag gttcctagcc tttatttaag tattacccca cagacccttc catatagatt
   104281 ttcaaattac acactagttt aagataaaac aaacaaggaa aaacttacca tttactcaaa
   104341 aaattaactc atataagtac attagaatgt aaaatagact ggtcaaaatc tggtcaaaaa
   104401 agtagatgtg acacaagttc gcattcaaag cgatgatcca tgatttcgta atggtcaatt
   104461 atatggatca aagccacgcg acaagagcgc aatggatgtc tctaccgatt ggttgaataa
   104521 acgaaataaa tagaatgtga aaaaggatcg gacaaatgtt ccagtttgct cgatcctttt
   104581 ttatttgaat tcattttcgc tttatcaaac atcatttgat tactcagtta aatacttttg
   104641 acatgagtac caatgaaaca tttattcatt aaaattactt atttcttatc gcctttctag
   104701 ccaatgccaa tccgccgatt attccagcac tatcacctaa ttcgggcgta acaatgtagc
   104761 tagcaattgg tggtatttca acatatccat ttataagctc aacaaattta tcctgaactt
   104821 ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta tctggcgaaa
   104881 gcatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc tgccaaaacg
   104941 aatcgtcagc caataaattc tggcctttta cacccaatcg tttttcaatc gctggtcctg
   105001 ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa ttgtcctttg
   105061 gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc acgaaagtcc
   105121 cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca acacctgcta
   105181 accctttgcc agaaccagca acatattcac catagcaagc cgcattaaca tctgttgtcc
   105241 attcaatcgg aatattgaaa tgttgcttta gcgttcctac aaaattgaaa ttctgccaag
   105301 caagtttcgg tgttgatgtg atatgaccgt atttctttga tttacgttga atatcaattg
   105361 gaccaaaaca gccgaccccg attcctgcca attgttcctt aaaaggttta aaaaaatcaa
   105421 tgacctgctt cattgtttca actggagtcg ttgtgggaaa actgactctt tccaaaatcg
   105481 tcaattcctc agtactgact gcacaaataa atttagtccc tccagcttca attgccccat
   105541 atatcatcat aggcttctcc cttcgaatat gtgtaaaaca cttcattaac gcggcaagcg
   105601 ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat accaata
//
LOCUS       DACDRN010000005       100810 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDRN010000005 DACDRN010000000
VERSION     DACDRN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 100810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.005"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(51..2120)
                     /gene="pknB"
                     /locus_tag="IWJ80_000482"
     CDS             complement(51..2120)
                     /gene="pknB"
                     /locus_tag="IWJ80_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP9460249.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(2117..2857)
                     /locus_tag="IWJ80_000483"
     CDS             complement(2117..2857)
                     /locus_tag="IWJ80_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP9460250.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(2870..4228)
                     /gene="rsmB"
                     /locus_tag="IWJ80_000484"
     CDS             complement(2870..4228)
                     /gene="rsmB"
                     /locus_tag="IWJ80_000484"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP9460251.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(4228..5166)
                     /locus_tag="IWJ80_000485"
     CDS             complement(4228..5166)
                     /locus_tag="IWJ80_000485"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP9460252.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(5159..5650)
                     /gene="def"
                     /locus_tag="IWJ80_000486"
     CDS             complement(5159..5650)
                     /gene="def"
                     /locus_tag="IWJ80_000486"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9460253.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(5665..8073)
                     /gene="priA"
                     /locus_tag="IWJ80_000487"
     CDS             complement(5665..8073)
                     /gene="priA"
                     /locus_tag="IWJ80_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP9460254.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(8326..8631)
                     /locus_tag="IWJ80_000488"
     CDS             complement(8326..8631)
                     /locus_tag="IWJ80_000488"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9460255.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(8636..9250)
                     /gene="gmk"
                     /locus_tag="IWJ80_000489"
     CDS             complement(8636..9250)
                     /gene="gmk"
                     /locus_tag="IWJ80_000489"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP9460256.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(9737..10021)
                     /locus_tag="IWJ80_000490"
     CDS             complement(9737..10021)
                     /locus_tag="IWJ80_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460257.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(10039..10929)
                     /locus_tag="IWJ80_000491"
     CDS             complement(10039..10929)
                     /locus_tag="IWJ80_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP9460258.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(10949..11167)
                     /locus_tag="IWJ80_000492"
     CDS             complement(10949..11167)
                     /locus_tag="IWJ80_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460259.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(11137..12837)
                     /locus_tag="IWJ80_000493"
     CDS             complement(11137..12837)
                     /locus_tag="IWJ80_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP9460260.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            13103..13876
                     /locus_tag="IWJ80_000494"
     CDS             13103..13876
                     /locus_tag="IWJ80_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460261.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(13957..14526)
                     /gene="thiT"
                     /locus_tag="IWJ80_000495"
     CDS             complement(13957..14526)
                     /gene="thiT"
                     /locus_tag="IWJ80_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP9460262.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(14592..14694)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(14700..15671)
                     /locus_tag="IWJ80_000496"
     CDS             complement(14700..15671)
                     /locus_tag="IWJ80_000496"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9460263.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(15765..17138)
                     /gene="glmU"
                     /locus_tag="IWJ80_000497"
     CDS             complement(15765..17138)
                     /gene="glmU"
                     /locus_tag="IWJ80_000497"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP9460264.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(17641..18459)
                     /gene="purR"
                     /locus_tag="IWJ80_000498"
     CDS             complement(17641..18459)
                     /gene="purR"
                     /locus_tag="IWJ80_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP9460265.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(18626..19450)
                     /locus_tag="IWJ80_000499"
     CDS             complement(18626..19450)
                     /locus_tag="IWJ80_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9460266.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(19423..20103)
                     /locus_tag="IWJ80_000500"
     CDS             complement(19423..20103)
                     /locus_tag="IWJ80_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9460267.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(20126..21064)
                     /locus_tag="IWJ80_000501"
     CDS             complement(20126..21064)
                     /locus_tag="IWJ80_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP9460268.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(21309..22160)
                     /locus_tag="IWJ80_000502"
     CDS             complement(21309..22160)
                     /locus_tag="IWJ80_000502"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP9460269.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(22314..24887)
                     /gene="mprF"
                     /locus_tag="IWJ80_000503"
     CDS             complement(22314..24887)
                     /gene="mprF"
                     /locus_tag="IWJ80_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9460270.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            25028..25834
                     /gene="proC"
                     /locus_tag="IWJ80_000504"
     CDS             25028..25834
                     /gene="proC"
                     /locus_tag="IWJ80_000504"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP9460271.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(25913..27469)
                     /gene="rny"
                     /locus_tag="IWJ80_000505"
     CDS             complement(25913..27469)
                     /gene="rny"
                     /locus_tag="IWJ80_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP9460272.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(27880..28929)
                     /gene="recA"
                     /locus_tag="IWJ80_000506"
     CDS             complement(27880..28929)
                     /gene="recA"
                     /locus_tag="IWJ80_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP9460273.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(29038..30279)
                     /locus_tag="IWJ80_000507"
     CDS             complement(29038..30279)
                     /locus_tag="IWJ80_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP9460274.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(30519..31097)
                     /gene="pgsA"
                     /locus_tag="IWJ80_000508"
     CDS             complement(30519..31097)
                     /gene="pgsA"
                     /locus_tag="IWJ80_000508"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP9460275.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(31306..32190)
                     /locus_tag="IWJ80_000509"
     CDS             complement(31306..32190)
                     /locus_tag="IWJ80_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9460276.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(32968..34254)
                     /locus_tag="IWJ80_000510"
     CDS             complement(32968..34254)
                     /locus_tag="IWJ80_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9460277.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(34247..35515)
                     /locus_tag="IWJ80_000511"
     CDS             complement(34247..35515)
                     /locus_tag="IWJ80_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9460278.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            35767..36228
                     /gene="mscL"
                     /locus_tag="IWJ80_000512"
     CDS             35767..36228
                     /gene="mscL"
                     /locus_tag="IWJ80_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP9460279.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(36287..36733)
                     /locus_tag="IWJ80_000513"
     CDS             complement(36287..36733)
                     /locus_tag="IWJ80_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9460280.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(36826..37071)
                     /locus_tag="IWJ80_000514"
     CDS             complement(36826..37071)
                     /locus_tag="IWJ80_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460281.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(37186..37764)
                     /locus_tag="IWJ80_000515"
     CDS             complement(37186..37764)
                     /locus_tag="IWJ80_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9460282.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(37841..38371)
                     /locus_tag="IWJ80_000516"
     CDS             complement(37841..38371)
                     /locus_tag="IWJ80_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP9460283.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(38375..39235)
                     /gene="rlmB"
                     /locus_tag="IWJ80_000517"
     CDS             complement(38375..39235)
                     /gene="rlmB"
                     /locus_tag="IWJ80_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP9460284.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(39228..39620)
                     /locus_tag="IWJ80_000518"
     CDS             complement(39228..39620)
                     /locus_tag="IWJ80_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HAP9460285.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEALM
                     GYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(39617..41029)
                     /locus_tag="IWJ80_000519"
     CDS             complement(39617..41029)
                     /locus_tag="IWJ80_000519"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP9460286.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(41026..41562)
                     /gene="cysE"
                     /locus_tag="IWJ80_000520"
     CDS             complement(41026..41562)
                     /gene="cysE"
                     /locus_tag="IWJ80_000520"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP9460287.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(41884..43344)
                     /locus_tag="IWJ80_000521"
     CDS             complement(41884..43344)
                     /locus_tag="IWJ80_000521"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP9460288.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(43514..44671)
                     /locus_tag="IWJ80_000522"
     CDS             complement(43514..44671)
                     /locus_tag="IWJ80_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP9460289.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(44705..46075)
                     /gene="radA"
                     /locus_tag="IWJ80_000523"
     CDS             complement(44705..46075)
                     /gene="radA"
                     /locus_tag="IWJ80_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP9460290.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(46138..46665)
                     /locus_tag="IWJ80_000524"
     CDS             complement(46138..46665)
                     /locus_tag="IWJ80_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP9460291.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            46895..47701
                     /gene="proB"
                     /locus_tag="IWJ80_000525"
     CDS             46895..47701
                     /gene="proB"
                     /locus_tag="IWJ80_000525"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP9460292.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            47698..48945
                     /locus_tag="IWJ80_000526"
     CDS             47698..48945
                     /locus_tag="IWJ80_000526"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP9460293.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            49021..49365
                     /locus_tag="IWJ80_000527"
     CDS             49021..49365
                     /locus_tag="IWJ80_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460294.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(49482..49808)
                     /locus_tag="IWJ80_000528"
     CDS             complement(49482..49808)
                     /locus_tag="IWJ80_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460295.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(50181..51382)
                     /locus_tag="IWJ80_000529"
                     /pseudo
     CDS             complement(50181..51382)
                     /locus_tag="IWJ80_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            51499..52014
                     /locus_tag="IWJ80_000530"
     CDS             51499..52014
                     /locus_tag="IWJ80_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9460296.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(52213..52659)
                     /locus_tag="IWJ80_000531"
     CDS             complement(52213..52659)
                     /locus_tag="IWJ80_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9460297.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(52706..54184)
                     /gene="msr"
                     /locus_tag="IWJ80_000532"
     CDS             complement(52706..54184)
                     /gene="msr"
                     /locus_tag="IWJ80_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9460298.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(54538..55167)
                     /locus_tag="IWJ80_000533"
     CDS             complement(54538..55167)
                     /locus_tag="IWJ80_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9460299.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(55151..55615)
                     /locus_tag="IWJ80_000534"
     CDS             complement(55151..55615)
                     /locus_tag="IWJ80_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9460300.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(56102..57370)
                     /locus_tag="IWJ80_000535"
     CDS             complement(56102..57370)
                     /locus_tag="IWJ80_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP9460301.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(57601..58338)
                     /locus_tag="IWJ80_000536"
     CDS             complement(57601..58338)
                     /locus_tag="IWJ80_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9460302.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(58405..58974)
                     /locus_tag="IWJ80_000537"
     CDS             complement(58405..58974)
                     /locus_tag="IWJ80_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP9460303.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            59102..59974
                     /locus_tag="IWJ80_000538"
     CDS             59102..59974
                     /locus_tag="IWJ80_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9460304.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            60316..61188
                     /locus_tag="IWJ80_000539"
     CDS             60316..61188
                     /locus_tag="IWJ80_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9460305.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            61185..63143
                     /locus_tag="IWJ80_000540"
     CDS             61185..63143
                     /locus_tag="IWJ80_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9460306.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(63394..64245)
                     /locus_tag="IWJ80_000541"
     CDS             complement(63394..64245)
                     /locus_tag="IWJ80_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9460307.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            64368..64835
                     /locus_tag="IWJ80_000542"
     CDS             64368..64835
                     /locus_tag="IWJ80_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP9460308.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(65098..65580)
                     /locus_tag="IWJ80_000543"
     CDS             complement(65098..65580)
                     /locus_tag="IWJ80_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9460309.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(65680..66543)
                     /locus_tag="IWJ80_000544"
     CDS             complement(65680..66543)
                     /locus_tag="IWJ80_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9460310.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            67141..67989
                     /locus_tag="IWJ80_000545"
     CDS             67141..67989
                     /locus_tag="IWJ80_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460311.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(68160..68501)
                     /locus_tag="IWJ80_000546"
     CDS             complement(68160..68501)
                     /locus_tag="IWJ80_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP9460312.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            68587..69294
                     /locus_tag="IWJ80_000547"
     CDS             68587..69294
                     /locus_tag="IWJ80_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP9460313.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            69281..69604
                     /locus_tag="IWJ80_000548"
     CDS             69281..69604
                     /locus_tag="IWJ80_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9460314.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(69693..70730)
                     /locus_tag="IWJ80_000549"
     CDS             complement(69693..70730)
                     /locus_tag="IWJ80_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9460315.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(71177..72160)
                     /locus_tag="IWJ80_000550"
     CDS             complement(71177..72160)
                     /locus_tag="IWJ80_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP9460316.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(72576..73346)
                     /locus_tag="IWJ80_000551"
     CDS             complement(72576..73346)
                     /locus_tag="IWJ80_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9460317.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(73339..74274)
                     /locus_tag="IWJ80_000552"
     CDS             complement(73339..74274)
                     /locus_tag="IWJ80_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9460318.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(74868..76517)
                     /locus_tag="IWJ80_000553"
     CDS             complement(74868..76517)
                     /locus_tag="IWJ80_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9460319.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(76712..77608)
                     /locus_tag="IWJ80_000554"
     CDS             complement(76712..77608)
                     /locus_tag="IWJ80_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9460320.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(77601..78281)
                     /locus_tag="IWJ80_000555"
     CDS             complement(77601..78281)
                     /locus_tag="IWJ80_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9460321.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(78282..79064)
                     /locus_tag="IWJ80_000556"
     CDS             complement(78282..79064)
                     /locus_tag="IWJ80_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9460322.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(79057..79974)
                     /locus_tag="IWJ80_000557"
     CDS             complement(79057..79974)
                     /locus_tag="IWJ80_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9460323.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(79977..80261)
                     /locus_tag="IWJ80_000558"
     CDS             complement(79977..80261)
                     /locus_tag="IWJ80_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9460324.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            81049..84276
                     /gene="fss3"
                     /locus_tag="IWJ80_000559"
     CDS             81049..84276
                     /gene="fss3"
                     /locus_tag="IWJ80_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP9460325.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            84388..84702
                     /locus_tag="IWJ80_000560"
     CDS             84388..84702
                     /locus_tag="IWJ80_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9460326.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            84715..85089
                     /locus_tag="IWJ80_000561"
     CDS             84715..85089
                     /locus_tag="IWJ80_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9460327.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            85099..85443
                     /locus_tag="IWJ80_000562"
     CDS             85099..85443
                     /locus_tag="IWJ80_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP9460328.1"
                     /translation="MEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            85512..86528
                     /locus_tag="IWJ80_000563"
     CDS             85512..86528
                     /locus_tag="IWJ80_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9460329.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLSALFIGWMGVFYFPELRQFGL
                     SKVSFSTIEIAWSMKDLVCLLGSLALSGSMMLLYIHFFPDHWRSLWNRQKLARMILEN
                     HWYEVKQTQSDGFFKDLNRSRTKETISYFPKVYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISKCVEDFYERMMARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            87122..88996
                     /gene="ltrA"
                     /locus_tag="IWJ80_000564"
     CDS             87122..88996
                     /gene="ltrA"
                     /locus_tag="IWJ80_000564"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9460330.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            89122..89478
                     /locus_tag="IWJ80_000565"
     CDS             89122..89478
                     /locus_tag="IWJ80_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP9460331.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            89523..90665
                     /gene="dcm"
                     /locus_tag="IWJ80_000566"
     CDS             89523..90665
                     /gene="dcm"
                     /locus_tag="IWJ80_000566"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP9460332.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            90655..90944
                     /locus_tag="IWJ80_000567"
                     /pseudo
     CDS             90655..90944
                     /locus_tag="IWJ80_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            91200..92384
                     /locus_tag="IWJ80_000568"
     CDS             91200..92384
                     /locus_tag="IWJ80_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9460333.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            92399..92494
                     /locus_tag="IWJ80_000569"
     CDS             92399..92494
                     /locus_tag="IWJ80_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP9460334.1"
                     /translation="MEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
     gene            92523..92744
                     /locus_tag="IWJ80_000570"
     CDS             92523..92744
                     /locus_tag="IWJ80_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460335.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            92757..93260
                     /locus_tag="IWJ80_000571"
     CDS             92757..93260
                     /locus_tag="IWJ80_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9460336.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            93386..94423
                     /locus_tag="IWJ80_000572"
     CDS             93386..94423
                     /locus_tag="IWJ80_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460337.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            94438..96135
                     /locus_tag="IWJ80_000573"
     CDS             94438..96135
                     /locus_tag="IWJ80_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HAP9460338.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKFPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            96128..96589
                     /locus_tag="IWJ80_000574"
     CDS             96128..96589
                     /locus_tag="IWJ80_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460339.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            96602..97462
                     /locus_tag="IWJ80_000575"
     CDS             96602..97462
                     /locus_tag="IWJ80_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460340.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            97500..97889
                     /locus_tag="IWJ80_000576"
     CDS             97500..97889
                     /locus_tag="IWJ80_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9460341.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            97876..100323
                     /locus_tag="IWJ80_000577"
     CDS             97876..100323
                     /locus_tag="IWJ80_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9460342.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            100280..>100810
                     /locus_tag="IWJ80_000578"
     CDS             100280..>100810
                     /locus_tag="IWJ80_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9460343.1"
                     /translation="MLLIPVRQFKREWSEEMKKKLFRIFGIVLISLVTLIILLSLVGT
                     VAEATGLVDDTVGAGNLYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAI
                     TNFIWTVSLYLSNATGYVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFG
                     FLLLMILALGVYVAYTG"
BASE COUNT        29557 a        20561 c        18718 g        31974 t
ORIGIN      
        1 aatgcaacaa atcaatgtcg cattcccttc tgatatattt tattcagcac ttattttgaa
       61 ctttctgagg tgctagctga tgaacttgtg gagctggaag attcttcttt tgaagagttg
      121 cttgtgctac tggagctact tgagctgcta gaactgtctg aagagtcttt ttcagaacta
      181 gaagacgctg gatctggtcc tttagagtaa attacgttca caaccattcc tttggtaatt
      241 gtcgttcctg cccctggtac tgtacgaatc actttatctt tatccaccgt gtttgaatat
      301 tcggaagtct catgcccgat atattctgca ccgacactag ctaaataact ttgtgcttcg
      361 ctttttgtat agccggagat atcattcaat ttaggctctg ttccttggct tacgacaaaa
      421 ttgatgtaag tttcttctgc tacaacttca cccgaggctg gatcctgact gatgatcgaa
      481 cctgatggta cagtgtcgct atagtcataa gtagcactaa tattattgat attgaatcct
      541 tgtgtaacaa gccaattacg aacagtttcg tagttttctc ctacatgact actcaaagtc
      601 actgtttctg gtcctttgct gactgtgaaa gtaatcgtgt ctgttttagg atcgacttct
      661 gtatcttctg atggagattg cgaaatgatc attccgcttt ctacctctga actgaattcc
      721 tcttcttttt gaatcaagtc agcagaaaac cctaattctt tcaaacttgc ttcggcatct
      781 tcataacttt taccactgta atcatctaat ttgatttttt tcgtgccgct actaatatac
      841 agagtgactt cgcgtttttg cttgactgtc gttcccgctg ccgggtctgt cttcactaca
      901 ttaccttctt cgattttgtc atctgcaatt tcttctgttt ttgttgctac ttctaatttc
      961 gcttttgcga gtgtttctct agcagaagct tcagaaagat cagaaacatc aggaacaaca
     1021 acttctcctc gcccgccaga agcaaagaat acagctgttc cgattcctaa aacagcaaga
     1081 atagctaata ggatcagcca ccattttttc tgtcctttct ttttaggatc aggcagaact
     1141 tcttcctcct ctggtaaggt ttcgttttct ggcttgtctt ttggcagagg catagatttg
     1201 aatgaatcag gcattggtgc atcttccgta aggggtgtga taacttttgt ttcattatcc
     1261 atcacatgag gctgccattt ttcttcgttt gcacgagctg gtgataatac tgtagaaaga
     1321 tcatcagaca tttcctcggc tgacttataa cggtctgcag gttctttagc cgttgcccgt
     1381 aaaatcacgt tttccagtgc ttgtggcaca tttggatcaa gtgcttttat agatggaaga
     1441 tcatcttgaa aatgttttaa agcaattgtt acagcagatt caccatcaaa cggtacgcta
     1501 cccgtcaaca tctcataaag gatgatccct aaagcataaa tatctgactg cttcgttgcc
     1561 atacttcctc tggcctgctc aggggacaga taatgaacag atcctagcat cgtattcgtc
     1621 tgcgtgatcg atgtttcaga taaagcgata gcaatcccga aatccgtgat ttttacaaca
     1681 ccttcattat cgatcagtac gttttggggt ttcaaatctc ggtggatgat ttgatggtta
     1741 tgagctaaag aaatagcaga aaggatctgc tgcataatat ttactgctgt ttcataagga
     1801 acaggataat gtgtttggat atatcgtttc aagtccattc ctttgacgta ttccatcacc
     1861 agatactgca tgttgtcttc ttcaccaaca tcgtaaacac taacaatgtt tggatgaacg
     1921 agttctgttg ctgctaaagc ttctctctga aatcgtcgaa tcgcagtctg gtcattttga
     1981 aagtcaaatc gtaagacttt aactgctaca tcacgatcca agattaaatc gtgcgccaaa
     2041 aatacatttg ccataccacc gctaccgata tttccagtga tttgataacg gccatttaat
     2101 tttttgccca actcgatcat acactgtcct ccttatattc gataagaaga acggtgatat
     2161 tatccgctcc gcctgcttca ttggcacgat caatcagctc agttacttta tgagacaatg
     2221 ggccttcttg attgatgatc gttcctattt cttcttcaga cagcatgttc gtcaagccat
     2281 ctgaacataa cagcagtcga tcttttagtt gccaaagatg agtagaaaca tctacttcga
     2341 ctgttccagg catgccgaca gaacgtgtta aaatattttt ccttggatgg ttcactgcca
     2401 tttcttcgct gatttctcct gatttgacta gttcattgac taaagaatga tcctccgtca
     2461 attgcatgat tttatcttca cgaatcaaat aagctctgct atccccaaca ttggcaatcg
     2521 tgaaccagtc atctgacaga gcggccgcta cgattgttgt tcccataccg ttatattccg
     2581 gctgttcttg tccacgctga taaatcagca tattttcttc ttgaatcgat tgaatgaacc
     2641 attgggcgat tttttcatcg tcaacagctt cttgtttttc ccaagcttcg cctagattcg
     2701 ttaccgccat ctgactggca atgtctccgg cacgatgacc acccatcccg tctgctaata
     2761 cagcaagctt gagacctgaa cggctggtaa atacactggc atagtcttga tttgtgttac
     2821 gtcgtcttcc tacatctgat tgatattcaa tctgcatttt ttcacctcat tactttttgc
     2881 gcaaacaaca aatgaaaaat ccatctgtgt aaaactgatg cggatacaaa gtcaacattt
     2941 tctcttcgat tgatgattgt agcataggat tggtcaaaac atcaatcctc tcaaaatcag
     3001 ggtgtctttc caagaaagca gaaacaacgt cttggttttc ttcttttaag atcgtacaag
     3061 tgctataagt cagaataccg gaagacttta aagttggtgc gcagctttct aaaatttcca
     3121 attggatttt tggtaaattc gctaattcat ccggctgttt tttataacga atatctggtt
     3181 ttcgccgaag cagcccaaga cctgaacaag gcgcgtctac taaaatccga tcaaactgct
     3241 ctgcttcgaa ctgtttactg acttctctag catccatctt ttgagcgtag acagcagaag
     3301 agacacctag acgtgctgca ttatcttcaa tgagtttgac tttgtgttcg tgtacatcta
     3361 aagcgaccac ttctccaccg catgacggat ctaaaaaaga agcaatatgc gtcgtcttac
     3421 ctccgggtgc tgcacaggca tctaagacat gatgttcagg ctcgatttgc atagacggcg
     3481 ctacaagcat cgaactttca tcttggatcg tcatgacgcc atcgtggaac aatgagctag
     3541 aagctaaatg cccttttttc gctactatac cgtatggact gacttggctt ttttcagctg
     3601 tgatctcttc ttcttgcaaa acagtgatgg cctgatctct ggaaagtcgg cgtgtatcta
     3661 ctcgggcact agcatggcta ggctgatata gcgataaacc gagcttttca gtttcttcgt
     3721 atccgatttg atccaccagt cgtttagtca tccatagagg caggctgatt ttgacagaaa
     3781 gtctttcgat aggatcagca atgccttgca gatctggtgc tccttgacgc tgataattcc
     3841 tcaagacacc atttacaaat ttccctgtcc caggatttcc ttttactttt gcgatttcga
     3901 ccgcctcgtt taaaatagca tgtgcaggga ctttatctaa aaattctaat tggtatacag
     3961 aaagataaag aagcgtcttg acccagtcat ccactttttt tgctttagcg atcaatggtg
     4021 caacgtaaaa ttccaatagt cgctgatggc taatcgtacc atagatcaat tctgtaaata
     4081 aacccacatc tttttgattt agctcgcttt gtttgatcat ctcattcaac aaaagattcg
     4141 agtaggcgcc acccttgttc actcgctcta atgtcagtaa tgccatatat cgtactgatt
     4201 ttttcatttt tttagggatt tttttcatta tttcacctgc tgaccaatct gaacttgctg
     4261 accgcttccg ttaaggaaat catgtacggc ctgttttcct ttaccagctg gctgtaattc
     4321 attgatggct aagacggtct gtttaccgca agcaatccat agattttttt tgtctttccg
     4381 gatgatcgta ccaggtgctt tgtctgtagt ttctgccaat acttcaaccg ataataattt
     4441 ccagcgtatg ccctcatagg tcgtgaatgc aattggccaa ggacgcattc cgcgtacttg
     4501 gttgtcaatt tcttctgcag ttttatgcca atcgattgct tcttgttccc gtgtgatgtt
     4561 tggcgaaaaa gttgcttctg actcgtcttg aggtactggt ttttgtccat ccaaaatatt
     4621 gggcaatgtt tctagcaata gctttcttcc taaaaggcta agcttttcaa acatcgtccc
     4681 tacatcatct tgtttagtga ttggtatgct ttcttgcgca tagattccgc ctgcatccat
     4741 ttttttgatc atttccatga tcgttacacc agtttctttc tccccattca tgatggcata
     4801 atgaacaggc gcacctcccc gatatttagg aagtaaagaa gcatggacat tgattgcccc
     4861 taattttggt acttgcaata acttttcagg caagaattgg ccaaatgccg cagtgacgat
     4921 cacatcaggc accaattcaa tgatttcttc catttctggc gaaccggaga ttttctctgg
     4981 ctgcagcaca cggataccgt gcttcagagc cgcttctttt accggtgtcg gcgtaattac
     5041 tcgtttccgc ccaactggac ggtcgggctg tgttaccact gcctggatct cgtaacctga
     5101 ttctatcagc ccttcaagaa tcggcacaga aaaagcaggt gtgcccataa atactagctt
     5161 agtcatcaag atgttcctcc atatattctt caagttcttc ttccgggatc tgctgtatca
     5221 ttttctcaat gaacaagata ccgtctaagt gatcgatttc atgctggaag gcacgggcta
     5281 aatagccaaa agccgtcact tctatctctt ctccgtctcg atcataataa cgcacggtca
     5341 cttcatctgc tcgcttgacc gttccataga catgcggaat actgagacaa ccttctacgt
     5401 caaggctttc accttttgct tcgatgattt ctgggttaat cagttcaaat ttctcgcctt
     5461 catctacttc gatcacagca attctcttgt tttgtcctac ttgaggagca gcgatcccta
     5521 ttccgtcatt cgcaatcatt gtctcatata aattgtctaa taaagcgatt gtctcgtccg
     5581 tgattacatc gattggctga gcagttcttt ttaatttatc attagggtgg atcaatatag
     5641 gatagcgcat aatttggccc tttcttaaat aaagtttaat ggttcgttgt caattgccac
     5701 aaaaagacct tgtcgctgtt ctttctggct tccattcaaa atttcatcca aaacatgagg
     5761 aagattcggt tcatgtttat atttgatgat tagttgataa taatatcggt ttttcactct
     5821 caaaatcatg ctgggcgttg gccctaatag caagctttga gggcttaaca cttcttttag
     5881 ctgattggca atctgaaaga ttttctttgc cgctacttgt tcttcaggat ggctggcggt
     5941 tattttcaca gtaaagtaat aaggaggata gcctccacga tgccgtacat gcatttcctg
     6001 ctgataaaac tgctcataat tttgtttttt agccaattct atggcgtaat gttgaggatt
     6061 gaatgtttgg atcacgactt cgcctgcttt ttccgctcgc cctgctcgtc ctgaaacttg
     6121 ggtaagcaat tggaatgtat gctcactaga acgaaaatcc ggtaaattca gggaggtatc
     6181 agcattcaac accccaacta atgtcacttc tggaaagtcc aaacctttag caatcatttg
     6241 tgtccctaat aatatatctg cttcttttgc accgaatttt tgtaaaatct gttcgtgtgc
     6301 cccttttctt cgagtggtat ctacatccat acgcaaaatc ctagcttgcg gataaagttc
     6361 tcggagttcc tcttctactt tttgagtacc tgttccatag tagcggattt tattgccgcc
     6421 acaattagga cagcgattgg gaataggttc ttcatgtccg caataatggc agcgcattga
     6481 acgtgtatcc atatgcagtg taagagagat atcgcaatta ggacatggaa gaacaaatcc
     6541 acagtcccta cacatcacaa aagacgaata tcctcgacga ttgagcagta ggacagtttg
     6601 ttcttttttg tctaatcgat tttgtatttt ttcctgcaaa ttagcagaaa aagtagaggt
     6661 ccgatgattc tgaaactctt cacgcatatc tacgatctca atagctggaa gctgtgcagc
     6721 tgctttggct cggtggttta actgcaataa ctgataaacg cctttttgag ctcgtgctct
     6781 tgattctaaa gagggcgtcg cgcttcctag aactattggg caatgatgat atttactgcg
     6841 ccaaattgct agatctctcg catgataacg cggtgtctca tcttgtttgt aacttgattc
     6901 atgttcttca tcgataatga tcagcccgat atttttcagc ggggcaaaaa tagcagatcg
     6961 tgcgcctact actacttgcg cttcttcccg ctcaatcttg cgccattcat catatttttc
     7021 accttgggaa agtccgctat gtaagaccgc aactgattct ccgaagcgtc ctttgaatcg
     7081 ctcgaccatt tgcggagtca atgcgatttc aggcactaac ataatagccg ttttattttc
     7141 tgacaaaaca tcagcgattg cctgaagata tacttctgtt ttcccgctgc cagtgattcc
     7201 ctccagcaaa aagacctgat ctttttgctg ctgacctgct gtgatgattt gttcgacagc
     7261 atttttttgt tctgcattca gttccaatgc cgtcgtttgg tcaaatactc ggtctttata
     7321 agggtcgcga taacgttctg attctataaa ctccagccag cccttttctt ttccttgatt
     7381 caagacagca gtactgattc ctttattttt gaaataagca attggttctt cttggctcag
     7441 ttcagataaa cacaaaagaa gttctttttg tttcttggca ttttgccgta attttgccca
     7501 ttcttcttcg atttgctttt cagttaacaa agaacggacc aaacgaatcg tttttacttt
     7561 atttcgtgta tggacttcat atttgatatc tactaattgc tcttggcgcc agcgaatcaa
     7621 ttgagaaagg ctgccttctt cttctgcttc cttccaggaa atcacttctc gctgatcaaa
     7681 caatacctga acttctggtg tgtctgccaa aggatagatc aatttatcat attctgcttt
     7741 catcacactc ggaagcatcg tttgtagaca agtgattttg aatgcgtaag taattttttt
     7801 catataatca gctaatgaaa gtaattcttc attgaccacc ggagcaaggt ctagcaaacg
     7861 aataactgcc ttcaaagatg gattagctga ttcttctgat ttttggatag ccataacaaa
     7921 tccttggaca tgacgattgc cattaccaaa agggacttct actcgcatcc cgacttgcaa
     7981 agctgtttcc atttcagagg gaaccaaata agtgaatggc tgatcggtct gcatagtagg
     8041 tacgtccaca atgacttcag ctgttaatgt catggttcgt ttcctccttt ttttgtcttt
     8101 tcttatcaag cgacaaaaag ttccttgcct attttactag agatcgatgg atttgtctta
     8161 tcttctgcca aatcctaaca aaaaattaac agcagataag aaaaaaaaga actgtgtcaa
     8221 aaagttcaga cacagccttt tctaccagac aataagtggc gaatgtttga aaaggaaaca
     8281 gctgggtttg cttttctttt tagatgttcg ctctattttt ctttattaca ttttttgctc
     8341 ttcacggata cgtgattcca attcgtgttg ttcacgctct ttttgtgctt gacgttcctc
     8401 ttcttccatc ttcaaacgtt cacgttttaa ttcaggatgt ggatcattga ctacatttcc
     8461 tgcatcgatt tcttctaaag cttgaccgac gctttttact gaatcaaaag aatctagagt
     8521 aggctgtgca cttgcatcca attcatgcgc gcgtttactt gctaaaatca ctagagaata
     8581 ttttgaattt acacgatcta atagtgagtc aattgaaggt tttaacatca taagactaca
     8641 tctcctttaa cattttaata tatttaccga ttacgcgatc tactcgaaaa tgttcactgg
     8701 caataatgtt tttgattcgt tcaactgcta gcggcacttc atcatttaca acagcatagt
     8761 cgtaaagagc catcatttcg atttcttctt ttgcaacttt cattcgttct tcgattactt
     8821 catctgcatc tgttcctcgg cctacgatcc gtgattttaa ttccgctaaa tctggcggag
     8881 tcaaaaagat gaatacacca tcgggcactt tttctttgac ttgttttgct ccttggactt
     8941 caatctccaa gaaaacatct tttccttcat ccaatgtctt gttgacatat gacaaaggag
     9001 ttccataata attcccgaca tattctgcgt attccagcat ctctccagct tcgatcattg
     9061 attcaaactc ttctttgctt cggaagaaat aatcaacgcc ttcgacctcc ccttcacgca
     9121 ttttacgggt agtcattgag atcgaatact gaaaatcatt atcctcactc tcaaaaattg
     9181 cttttcgtac tgtgcctttt cctacaccgg atggaccaga caacacgatt aataatccac
     9241 gctctgacat gacgacgtcc tttcaactag tctttttgta cttatcgttt attttgcact
     9301 ttttttctgc aactttcaag gttatcttta attatagcta gaaattggta aaaaagctac
     9361 cctgtaccaa gaggtcgtaa aaaagctgtc ctgcatcaag taataagaac agccaaacat
     9421 aaacagcttc tcatgttttt cgtttggttg gggttattat cgcagatgca agcttttgaa
     9481 cgccgtgtat aagaggtcgt gaagaagccg attagtttcg agtatgaaga aaaattttgt
     9541 gaaaatagtt tctcacattt ttgcataaat ttggcttaat accgagaaaa tggtttttga
     9601 acatcactta ttcaaaataa aaaaagattg tgacacgaat tttgtcacaa cctttaaatg
     9661 tattcctaga gcaaagaagc taagcgaaga aatccaattc tgattattta ttgtatttct
     9721 ttgttcagca tccttgctaa aagcccatat tggacaaagt attcactaca cccatgatct
     9781 catcttttcg agaacgtgct ttttctgtcg gatcttctac ctcttctgtg tcaattcgaa
     9841 caataaattt ccgattcatc gtcacgtaag caccgtcgtt gatcaaaata aacagttcgt
     9901 tctcattata gtctgcacag gcatctttcc atacgtcaaa gccgtttttt tcagaaggga
     9961 tatcccgttt aaccacagtt tttccactta attcgtggaa cgttacgtca taatatttca
    10021 ttatctctac ctctttcgtc attcaatatt ttgtacttgt tctctgattt tttctaagat
    10081 cgtcttagac tggacgactt gatttttaat ttcgattgca tttgatttgg aaccgatcgt
    10141 gttgatttcc cgattcattt cttggatcag gaaatctaat tcccgaccta caggacattg
    10201 tgcttcgata agttcatcta gtttttgtac atggatcgcc agacgatcca gttcctcgtg
    10261 aatatctccg cgttccaaca agatggctag ctccgtcaac aaccgttgct catcgacttt
    10321 cgctccaaga aattcctcca gctttttttg ataccttatt tgaaattcct tttcatagat
    10381 ttctacaaaa ctatcgagtt tttttagtac ttctttgaat tcttcagtat tctttttcaa
    10441 cactaaagct aaagcttgac cttctttttc tcgactcgcc tcaatagaat caagtgcgct
    10501 tttcaatgta tctagagcta actgtgccaa ttcttccatg ctctcttccg gtttttcttc
    10561 taccgttacg aagttttcgt ccagcgcgaa gcgttccgcc atccgactaa ttgacacttg
    10621 attttgacta ccgtatcgtt gttgaattcc gtctgtcaat tgagtgataa gtgaatcaat
    10681 caattcccag ttgatgacga tctgtttttc gtttttgcca atataagcta gattcacaaa
    10741 tacttcgatt cgaccgcggc ttaactgctg tttaataagc tgacgaaaat cgttttcaac
    10801 aaagttcagg attttaggac ttcgtatttg aacatctaga aaacgctggt tgacactttt
    10861 tatttcaata tctaactgat acgcttttgt ttcgcggatg gctttgccaa aaccagtcat
    10921 acttttcatt cgacagggtc cttcctcatt acatattttc tattgctttt tgcagttgtt
    10981 caaactcttc gttcgtcaat gtcaggcctt tccccatttt ttcatggtct ggcgcccaat
    11041 ctcgaagatc gaattttggc gggcgaccat tccagctgac taggttcagt tctttacgcc
    11101 accccttgtt gttttctgac aatatagcga tttcttctac gatttcatat gaaaattctt
    11161 gtgccatggt tgttcctcct tgagataata taaatatagg atcagtaatg tgtcaactgg
    11221 ttgcttaagc aattcatgct gcaaacgatc cttgcgtttt ccttgtagca gataatcaaa
    11281 ataaacagtt ttcgtgcgct cattttgtat tttttgctgg tattttcgat accttcgcca
    11341 ttcttcagta cttaattgac caatttgtgc caataaatca tccggcatac tttttatatc
    11401 ttgttggcag atcatttgaa tcatcatgtc tacttgatag atttcattgg gcagtaaaaa
    11461 atgttttttt gctaaaagtt ctagcatcgc caatccgcat gtctctggta cgaccctgtt
    11521 cttatataaa ttacgcagtt tttgccaaaa ttttatgctt ttgaaaaaag ataattctaa
    11581 ctgaatttct tttgttaagt aagacaaaaa tcctcgccag tatgttcttg caggtttaac
    11641 agaatgaaat gtataccagt tcccatagta atagcctaat cgttttttca tttccagata
    11701 gcctaattgt aaatttaatt ggttcctttg gctatcaaaa agcagaaagc tgcctagtgt
    11761 ccacaatgtt tgacatttcc aatggataaa aatgtctggc atacgaattt ttttcgctgg
    11821 gcctggccct tgcacatcta cgacaaatgc ctctgttgct cctttgttaa tcgcaatatc
    11881 aataggtatt ttgttgcgat acccaccgtc tgcatattta ttttttccga tctttcgata
    11941 agccatagcg ggataaaatg aagcagaggc gagtatccaa tctggtattt cttctgtttt
    12001 tgtctgttgg atatcagtca ctacttccgc tagttctggc agtttagtag acaccgtata
    12061 taaacgtatt ttgggattat gtttgagtaa ggacaaatcc aaattgtctt tgaccatctt
    12121 ttccaatgga gcagaagaaa ccccgccttc aattagtgct gttttcgtca tttggcgtct
    12181 ttctcgatag aagcgttcac gaaggtcttc aactgcagcc atttctggta acgcaagcac
    12241 ttgtcttgtc gacagctttt tccataaatt cacagctttt ttcatatctc cttgtaaaat
    12301 caatgcgccg ttcaacgcac ctaccgaggt acctgtgata atctcaaagt tgatatcatg
    12361 ttccttcagc gcctgccaca caccaatctg ataagcaccg tgtgctcctc ctccacctaa
    12421 aacaagtgct gtatgataga ccagcttttt atttaattgt gtggttcctt gatagccgtt
    12481 tttatgaaaa atacgcaacc attcttctga tagaggagat tgaaataaaa aacaacagtt
    12541 ttctttaata aaaaaacgag cgcaagcttc catataccga aaaaattttt tccattcaaa
    12601 agtttcacct tcaatcaaca gattattaaa aataacatct cctctacttt tagcaatagt
    12661 aaaatagacg gtatattttt tcgatactgc tttaaaagca cattgctttt ttgcagaacg
    12721 ccaaaaagcg tctctacttt cttgatgact ttgaaaaaat ggaaggggtt gttttgcttg
    12781 ctggataggc gaatacagcg acattttctg tgaaaagtaa acttttttta tctccatcgc
    12841 gctctccttt ttcttcaagt ataccagtac gtcataaaaa ggtcatgagt ctcgtattcg
    12901 actttgtaga ttccataaga aaacaattca taaaacatcc tttaaataaa aaatacaaaa
    12961 atcacaaaaa ttaattgaaa atgagagtgt tttcaatgaa aacgtgattt tgtaatggaa
    13021 ttttaaaata aaagaaacaa gcttttttaa tgcaaatgtt gtaaaataaa agagattaag
    13081 aggaaataag aggaggaaca tgatgactga gtcagtttat gtacatattg ataccacaag
    13141 taatgctgtt ttaacaaaag ggctgactgc tactgacttt gcaaacagta tcgtgcattt
    13201 tcctcaaaac ttgctcttgc ttgatccatc tgcttctgct ggagaatatg aatcccatac
    13261 agggctgaaa gtaatccgtg gtacagaaaa tattcagcgt ttcttttcct cttctcgtaa
    13321 caggcgtgga ttcgatgaat tgaagtggat cgactttact gatttaacga tgctgaaaga
    13381 attgacacct ttagaaattt cagaattatt atattttggt catatgaaaa cacatcttca
    13441 ttcgccgttt ttttataaac tacagaataa ttttgtatac ttcgatctta atgatcagct
    13501 gaatcggatt tattatcgct atttagatga attttatagg atttttgcta gtaaattaag
    13561 ttcgatcttg tcagataaga tcaatgtaaa aaaatctttt ttcaaaaaag aaacacctgt
    13621 tgaaaaaatc agtgtggaat tattgaaaga aatgcgtaat ttgatgcagg aagggatcat
    13681 tttttctttt gatcaagttg gtttgatcga tggtaaatat cgtatcccaa tctatgtgat
    13741 tgaagattat atttggaaat taaaaggcac ccggtatcga gaagaggaaa tcatcgctac
    13801 tttaatctac tctgtcaccg atcgacactg gcaattggta gaagatgccg atctttctta
    13861 tctgccattg aagtagatct atttcttatc aaaaaataaa aaggaaacaa gaaactagga
    13921 catgattctg tctcaagttt cttgcttctt ttttatttac agctcctgtc gtttatctgg
    13981 cacaaaaatc gaaggagtcg tttgttggat ccctaataaa acgatgatcg ttacaatagc
    14041 tgtactcaac ccgctggcac cattcattac gaaagaaaag agccaagaat tcatccccca
    14101 tagtgcatac gctccccaga aaaccactcc tgcaataaaa tgccaaaaat atctggaaag
    14161 tgcgccaatc aatgcaccaa acacgatgct ttttctaatt tgtttcgtat tatcgtctac
    14221 atagcctttt tggacatttg aagccatgag ccctgctgcg cctgcaaaag tataggcaat
    14281 cgggtactca atcaacactt gaacgacact taaataataa acttgtccca acggaaaatg
    14341 taggatgccc caaatgaaac ctgccagcat ccctgccttt gtccctcttc ttaacgcaaa
    14401 aagagtcaat ggaatctgcc ctaatgaaat agaaaaactg ctgccgatct ggatcggaat
    14461 caacgacaac accatcgcta acgctgcaac aatcgttcct tctacccaaa ctctattttt
    14521 tcccataaaa aaacttcctc ctttgaataa aaaagtcaca aaggaggaag cagctttcga
    14581 aaatagtcaa acagctgttt attcacaatt cctacgctcg cataaccgat acaggtacga
    14641 agggtttaga aatagattca atctcagccc aattggactc ccctttgtga gtttatttat
    14701 tatttatttt ttgtttcaaa caatggacta actggtttgt tttcatggat acgtttgatt
    14761 gcatctccca ttagtccgcc aacacttact tcatcgattt tgtcgatttt acgatcttct
    14821 ggaagataga tcgagtctgt aacaactaga cgctcgatag ctgaatcttc tattctttgt
    14881 aaagcagggc cggataatac tggatgtgta catgaagcat acacactagt tgcaccagct
    14941 tctttcaaag cattggcagc caatgtgatc gtacctgctg tatcgatcat atcatcgatc
    15001 agtacacaag ttttgccttt gacttgtcca atgatattca ttacttctgc aacatttgct
    15061 tttggacggc gtttgtctat gatcgcaatc ggagatttca aaaattccgc taatttacgt
    15121 gcccgtgtga caccaccatg gtctggtgaa acaacgacaa catcatcgcc tttgattcct
    15181 ttttctaaga aataattggc aataagtggc gcacccatca aatgatcgac tggaatatca
    15241 aagaaacctt ggatttggac tgcatgaagg tccaaagtca gcattcttgt tgcaccagct
    15301 ttttgaatca tgtttgctac taatttcgct gtgattggtt cccgtgcacg tgctttacgg
    15361 tcttgacgcg catagccata ataaggcatg actacgttga ttgtttttgc acttgctcgt
    15421 ttcagtgcgt caatcatgat cagcaactcc atcaagttat cattgactgg gctgctcgtt
    15481 gattgaataa catagacatg cgatccacga atactttctt caatgtttac ttgaatttct
    15541 ccatcactaa attgagtaac agatgatttt cctaactcga cgccgactgc tgctgcaatt
    15601 ttctctgcta atgggcgatt ggaatttaac gcaaaaattt ttaatcttgg gtcaaagtaa
    15661 tgttttgaca tggggacctc cactttcttt ttcacaactc tagaattcta cctaataaat
    15721 agtagtcata ttttttaaat aaatcaagtg attttgcaaa agtcttagtt tagataaggg
    15781 agtttttttg catatccttc tttattgact tgtcttgctc tcgcaattgc cagcgcatat
    15841 tctggaatat tgtctgtaat tgttgacccg gcagcaatcg aactgttttt agctacctcg
    15901 acaggtccga tgatattcgt gctagaacca ataaagctgt ggtcgccgac agtcgtatga
    15961 tgtttgttct ttccatcata gttgacgaag acaacaccac atccgacatt gatttcttct
    16021 cccagcgtag catcaccaac ataagtcaag tgaccgactt tagttccttt gccgatttga
    16081 gcatttttca cttccacaaa gtttccgata tggacgtttt cgccaatttc agcttttggt
    16141 cgtaaatgtg cataaggtcc cacatctgca tgtttcttga catggctgct ttcaatgact
    16201 gagttttcaa tgacgacgtg atcctcaatc ctactgtcta cgatttttga atgtgacccg
    16261 atgacacaat cagaaccaat gactgtattt ccttggattt gtacacctgc ttcgatcaat
    16321 gtgtctggac cgatttctac gcctgcatcg atgtatgtcg ttgctgaatc gacaaagctt
    16381 acgccattga ccatatgcat ctggttaatt cgctttctca tgatctcatt tgctttagct
    16441 aacgcgatcc gatcattgac tcccattgat tcttcaaaat catccgtttg ataagcagca
    16501 actgtatggc cttcttcttt taagatttcg atgatatctg ttagatagta ttcaccttga
    16561 gcattgtttg ttccaacttt gcttaacgca tcgaacaaag cttcgttgtc aaaacagtaa
    16621 gttcctgtat tgatttcttg tacaagagct tcttctggtg ttgcatcttt ttgttccacg
    16681 attttttcta cgatcccaat atggtcacgg atgattcgtc catatcctgt tgggttttct
    16741 gcttgtgccg tcaagatcgt tgcacttgca ttttttcctt ggtgatattc aaaaagattg
    16801 ttcagcgttt cagtagtcaa taatggtgta tcgccactga taactaacgt tgttcctttt
    16861 tttccttcca agaaagaagc agcttgcaaa acagcatgtc ctgttcccaa ttgttctgct
    16921 tgtaacgcat atttactgcg ttctcctagt tgtgctttaa cttgttctgc tccgtgtcca
    16981 acgatcgtaa tcacttcatc tggtttcgtt tcactgaccc gattaatgat atgttctacc
    17041 ataggtttac cagaaacggg atgtaaaact ttatataatt ttgacttcat gcgggtacct
    17101 ttccctgccg ccaaaataat tgcataacgt gcgtccaact gcgtcactcc taaatatagt
    17161 ttgctgattt cagtttatcc cagtataccc attttttcaa caaaacaagt gattttcttt
    17221 tatcccagcg ttttctggat aaaaaaatag atagaccata acgggcctat ctatttggta
    17281 accggtgctc tcctgccaac tcctcggtca caagtcacac tactcataaa acatgaaaag
    17341 ctgttttttg aaatcgtttc ttgtgactta gagttaaacg gtaggcttac aacctcttca
    17401 ttaaacgttt ttcaatcagc tgaccttcgg tattagctca ataaacggca aaatatgagg
    17461 agcaattttt ccgtttattg agctaatact caggacagga cgctgatttc acaacctcta
    17521 gataaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatga aaaaatgtgg
    17581 aaagccattt tcacaaaatt tttcttacta ctcgaaacta agcggctttt tcacaacctc
    17641 ttattctgaa aaataatttc ctggcacaac cgtaatattt ttcgtttgag tatctacatc
    17701 tttgacataa agcagtgacg tataatcatc gatggcgcga tgaccgttga attttgattc
    17761 ggcaaataca gtgatgccaa caagttctga ttcgaattct tcaatcaagc tcttcattcc
    17821 gttgactgtt ccgccgcctt tcatgaaatc atccactaca aggacacgtg aaccacgttt
    17881 caaacttctc ttagacagtt ccatcttttc aattctttca gaagatccag acacatagtt
    17941 gacacttact gttgatcctt ctgttatttt tgagtcccgg cgtacaataa cgaatggcgc
    18001 attcagataa taggacacag cttgggcaat aggtacgcct tttgtggcta cagtcattac
    18061 agcatcgatt ttttcaccaa ggtatttaga agcaatgatt cttccaactt gacgcagcag
    18121 ttcaggttct cctaacaaat cagagagata aacatatcct cctggaagca gacggtcttg
    18181 ttctgataga cgttctgcta gagattcaat atattttttc gcctcttcat atgaaatttc
    18241 agggatgaac aatacaccac ctgcagcacc cgggattgtt tccaaggttc cataccctcg
    18301 ttctttaaat gttttcttga tgattcccaa atcttcactg atagaagatt tagcagattc
    18361 ataacgttcg gcaaagcttg ttaaggaaat caattcatgc gggtgattca ataaatactg
    18421 tgtcatgtca actagacgtt cactgcgacg tactttcacc tattccaact ccttttaaga
    18481 aattttatcg ttttacatta tataagtttt tcttcaaaaa ttcgagcatt ttatgaaaaa
    18541 ataagaaaat agttcggtat ttattaaaat aaaaagaagc ctataaaaaa tttttcgttt
    18601 ttataaggct tcttttcttt ataatttagt tattttttct tctgaaggca accaatattc
    18661 gtttgatgat atttacaatc aggaacaaag caataaagac taatgtgatt gttgctcctg
    18721 gaggtgtatc tagataaaac gagctagtta gtcctgacag catccctaga aatcctacac
    18781 aaacactgat agcaatcacg acattgaagc ttttgcctaa tcgcataccg attgctgcgg
    18841 gaagaaccat gattgcggat atcaaaagtg caccggcaat cgggatcatc acagcaatag
    18901 ccacaccagt aatcacattg aacatcatcg acatcaaatg aacgggtagc ccatccacgt
    18961 gggcggtatc ttcatcaaaa gtcagaacat acatcggccg tttgaataaa aagaacaata
    19021 taccgatggc aacgaataag attcctaaaa agatgacttg ctcccatgta atggtcacga
    19081 tcgaaccaaa cagataggat tggatacttg ttgctgaatt ccctccgctc aaattcatca
    19141 atactaacgc tagcgccaaa ccaccagcca ttaaaatggc aatggagatt tctgaatagg
    19201 ttcggtagat cgttcgcaga tactccaata tcacagctgc taatacaacg actagcatgg
    19261 tcgtaagtgt aggattaagg ttcaaaaaga atcctaacgc tacccctgct aaagagacat
    19321 gagaaagcgt atcagccatc agcgattgtc ttctgatcac taaaaacaca cctagcatcg
    19381 gggcgattcc ggcaatgaag atagcggcaa gaaacgctct tctcatgaat tcatatgaaa
    19441 gcaacgccat tgagaatcct cctttctcac cagtcggatc tgtctgtccg catactgttt
    19501 gatatcttca tgatcatggg tgatcattag aactgatttc ccatggacat gcgcactatg
    19561 tcggagaaga cgataaaatt catttctgga ttcttcatcc atacctgttg ttggttcatc
    19621 aagaacaaat aagtcagggt cagtagcaaa aatacgcgct aaactgattc gctgcttttg
    19681 tccgccagac aattcaccta ttcgccgatt tcgcatatcc cacatcccta cagcttctaa
    19741 agcacgctgt acatgttgat gatctttttc agagagacgc ttgaaccagc ggcctcttgg
    19801 atatcttcct gaacggacta attcgatcac cgtactagga aaacctgcat tgaaagatgc
    19861 aacttgttga ggaatataac cgatacttaa tttttttcct tcttgatttt tctcagcaat
    19921 ctttatcatt ccttttgaag gtttcagtaa tcctaacgat gctttcacca aggttgactt
    19981 ggctgctcca ttttctccag ttaaaatgac aaattcaccc gcatctacat gataagagac
    20041 atcttctagt acaggttcat catcatagta aaaggtcaaa tcagatattt cgatataacg
    20101 caccttacaa cctccattct aaaacttaat tgatactttc ttttaaagct ttcagatttt
    20161 tttccatgac agaaatataa ttttcgcctg ctttgatttg ttcattagtc aagctttcta
    20221 aaggattcaa cacttctagc ttcactcctg tttcattcga caatgtctca gctaccttcg
    20281 aagaggcatt ttcttcaaaa taaatcactt tgatgttgtt tttcttcaca aattctttta
    20341 gctcagctaa gcgacttggt gtgggttcct gatctggtga aacgcccgaa atcgctactt
    20401 gttttaagtc gtattgattc gctaaataag caaaagcagc atgctgagtg acaaatgtgc
    20461 ggtttgttgc atcttttagc tcatcggagt atttttgatc cagtgcttca agctttttga
    20521 tataagcatc ggcattcttc gtaaaagttt cctttttatc tggatatttt tcgcctaatt
    20581 cttcagcaat cgttctgact tcttttatag ctaataccgg atcgagccaa acatgtggat
    20641 cgtattcatg tgaatgacca tgcccttctt ctgattcttc atgttcgtct gcttcttgcg
    20701 attctaaacg gtcgattcct ttagctgctt cgatcatcaa ggtctgtttg ctatccactg
    20761 aatcctttaa cgaatccaca aaaaattcca tgtctgaact attatacacg aaaacatctg
    20821 catcggaaat ttctgctctt tcttttgcac ttggttcgaa atcatgcggc tccgttccag
    20881 cagggatcaa aagtttcacg tttccttcat cccctacgat ctcttttgta aaatcataca
    20941 tcggataaaa agtcgtaacg atcgtcaaat cctctttttt gtctgcttcc ttgttcgtat
    21001 ttccacatgc tgcaaacagt aaagcaccta tcattaccgt agctgtcaaa agatattttt
    21061 tcatttatat gctcctttca atgaacaaat cggaatttta cgatttatta acgtgaacta
    21121 atgtatcaaa ataaaaaaac acagtcaata gaaatccatc aatttctgat ttaagcatgt
    21181 atattcttga ttaaatcgta aaatggatga gttgctgctc atatctagcc atttcactac
    21241 aataaaaaac tgaagaatac cattttagga ttcctcagtt tttcatcacc tattttactt
    21301 cttatatttt attgcagggt tcgtactaaa taaacttcct cacaaaaacc ttttaaccca
    21361 ttatatactc tctgcgctct tgaataatgt tgacacaagg caaaaacggt tggtccgctt
    21421 ccactcatca atgcagcatc tgctccgtac ttgatcatac gctctttgat ttgatggatg
    21481 actggatgcc gctttcccgt tatatcttct aaactgtttc ccattttttt gatcatctgc
    21541 tgataatctt gctctcggat ggcttcagca agtccgtgga tgtcttgatg ttggatggct
    21601 tgcgtatcta cttctctaaa cactgtacgt gtagagacac tcattcttgg tttgaccaac
    21661 acaacccagc actgtggcat agaaggtaat acttggattt cttccccttt gccaccgaca
    21721 aatgccgtac ttccgtagac acagtaagga acatctgtac cgacttccaa accgatttct
    21781 gccagttctt ctaacgaaca gcccagattc cataattggt tgatccctct tagagcagct
    21841 gcacagtctg tacttcctcc gcctaatcca gctgctactg gaatattctt cttaacaaag
    21901 atcttgacac cggttttgat attgtattta ttttttagca ataaagcagc ttgataaaca
    21961 ttgttccgtt catctaccgg taaaaatgct ttatttgttt ctacaatgat tttgtcttct
    22021 gttatttctt caaaaaatag atgatctgcc aagtctacgc tcgacatcac catttccaac
    22081 tcatgatacc catcttgccg tctatataat acatccagtc ctaaatttat cttggctggc
    22141 gctttttcaa tcaattccat cctctcaccc attcgaaaag tctaagtcgc aagtattcta
    22201 tcatagggaa aaataaaagt atagcttatg tctgcctagt ggttgatttt gtcatcgttt
    22261 caacagcttt cacagaattt tctcttcata atccaaacaa gttctagtga ttcttataat
    22321 tctgtgagca cttcttctgc caaactcgga acatttcctt tttcttttcc tatcagactg
    22381 agtagctgca tggtagcaaa tccgaaatta cttcctttcc tataagcaaa ataacgtcct
    22441 tcccaatgat cgacatattt taatttcttt tcataagcag actggaagga atagaaaaaa
    22501 ttaccataat tatatacgat atgcatgact ttttcttgaa agaaagaaaa atctgtttct
    22561 ccaacattag ctaatggagc taagcctaga ttggcttccg agtagcccat tttctcgtat
    22621 acaataaaca gattggctaa cagatagtca gctaattcct cagattcttt aggtaagacc
    22681 cgcaacaaat catatcccgc ctgcttcttc gtcatcggct gagcggtgat gaagccaacg
    22741 actttttgat ctttacggac aataccgaca ccgctagata atacatatga tttatcaaat
    22801 cgtcccacag agaagtattt ttctcgtgca ccttgaagcc atttttgtga aacttcattt
    22861 acagcttgat gtaattcttc cggcagttga tcgtaccaag taaaatggta tccttctttc
    22921 atcaaacgtt gatattcaag ttgctgactc agccgagaca agtcttctgt tgctagatca
    22981 acaatccctg cttcacctac tttcgtgaag tgaaaaccca attcatgaag catcactacg
    23041 tagctttctg atatcttata aaacgcaagc tgatagccta aacaatctgc ttgtttcata
    23101 aactgattcg ttgctgcccg catcatttta ggattgccaa ctggatctcc taaaacaaag
    23161 catttgtttc ctttgatctg ataaccaaaa acgacttgat cttggtcgtc ttcttggtaa
    23221 taaaaataac tgtaacctga caagcgaagc tgatggcttg tagctgtgcc tccgtatgtt
    23281 tccaataaac ggtcaaaacg gtctttttcg tactcactgc caagcttcgt agtatcttca
    23341 gctaaatatt gataaagcgc cactaaagct agcaatgaga tgcccaaacc aataagccct
    23401 gagaaccaca catcatctga aggaaacaaa ataaaatgat ttcctgttat tcgaccgact
    23461 aaaaagcgtc cgctgtgata gcctgcaatt gcgtaaacga taaacaaaaa gccgaacaag
    23521 ctagcatcaa agattatagc tccccaagaa taaacgaatt ttgttcgata aaattctttt
    23581 ctggataacc acacagctaa cagaatcaag acatatacca atatcaactg ccaagataac
    23641 gtacgtgtga ttgtatttaa aataccaaaa gatagtaaag caatcgtagg cagaaatgcc
    23701 tttttgactt ttactgaaag tgctctagca aggccgagta ataaaaagcc aaccaataga
    23761 ttcactgtct gatctaaaaa attgaatgag aacggcaata atacttgaaa tagccgactg
    23821 attgcagaca gattagtgat tgttgataga agtaccatca tgatccccgc aaaatagaca
    23881 gcacttacga gcacgatatg cgcaaccttt tggaaaaaca gtcgcggaat gttatctaaa
    23941 aatcgattga ttttgactcc tgtatgtgtg atgaacaata gaagtcccgt cataaaaggt
    24001 agaacataat aaaacaaccg ataatataat agccaaacta ctgctacatc ttgccgaaca
    24061 gacaattgac tcattcctaa gatcatcaaa acgtcaaaag tccccattcc acctgggacc
    24121 atcgtcagca ttcctatcaa tgtagcaatc atgaacatag gatagatcga aagcatcgaa
    24181 atatctattc tcatcaactt gccgatgatc aaaaataccg caagggctcc cgtccattgt
    24241 ccaaaggatg ccagtagcag accaagcaag ccttttggat aaaactcgaa gaacaatgtc
    24301 tttctcttta gataagcaaa aaagaaaatg actggtgcta aaaaactacc gccgaaaagc
    24361 cagatccaat agcttcgaaa aatactttct gactgaataa aaaagacatc aataaatgta
    24421 agaaacgacc atacagacaa accagtcagc ataaatagtg cgacttttga tacagtggct
    24481 aaaaccattt ttctatttgt ctgctttcca taaaaactag cccgcagact tgctcctacc
    24541 acaccaccaa aaccggcgag attgttgatc gtgtttgtta cccacgcagc aagaagaaac
    24601 tcttttcttt ccatttttgg ttttccttgt ttttctaaga ctctgatcgt cacccaatca
    24661 tacaacaaca tcggagtgac acccagcagt ccagccagaa tcatgagcag gatcgtcatg
    24721 cggctttgtt gtcccatcct ttgccaaata tcttgccaac tcatgccatt agcaatattt
    24781 gcgacttgat ttgccacaaa gactaacacg gagccgaaaa aaatcagttt taaaatcaga
    24841 ctatgttcct ttatccattg cagcatcaag tatcgtttat ttttcacatt ttcccattcc
    24901 taacttgtat acttttgtgt cataccccaa tgttatctta tttattttaa tttttccact
    24961 ttagacctgt tctttgcttg cgatgataga aaaagaaaga tatcctaatg ctaaaggaga
    25021 ggataacatg aaagtaggat ttatcggcac agggaatatg gcacaagcta ttattgatgg
    25081 tttattaaaa aaaggaacag tctcgaaaga agaggtgttc gtttccagtg gacattttga
    25141 aaatgcacaa aaatttgctg ctgaaactgg cgtccatgca tgcaaaagta atcaggaagt
    25201 tgcatcacaa tcagatatcg ttcttttagc cgtgaaacca aaaatcattg aaaaagtatt
    25261 ggaagaacta agagaatata gccagaaagt cttattcatt tctattgctt caggaaaatc
    25321 attaacagat ttagaaacag cgcttgaaga tcctcaggcc tccatcgtac gtgtgatgcc
    25381 gaatgtcaat gtcagcgtag gagcaggcgt ttctgcgatt tgcaaaaatg aagctgccac
    25441 ggaggaacag cttcagcaag ctacgatgat ttttgaagca atcggcagcg tctatccttt
    25501 agcagaatca gatttcagta cattcattgg ccttgctggc agctcacccg cattcgttta
    25561 catgctgatc gatgcgatgg gacggactgg tgtactgaat ggtttgtcca aacagacagc
    25621 cactgagatt gctgcaaaag caattatggg ttcttgtcaa aaattccttt caagtgataa
    25681 aaatccatgg gaacttgtag accaagtttc ttctcctggc ggtacaactg ttgcaggtgt
    25741 agttgcctta gaagaagcag gattcatccc agcaatcatc aaaggaatca atacgacgat
    25801 cgagaaagat cttgagatgt tgaataaaaa ataatgatag caatgttttt tcgtgcctta
    25861 caaaaaaaga agagctgtgg cttttgccgc ggctcttctt ttgtattttt tattatttag
    25921 cataatcagt tgcacgagtt tcccgaataa cgattacttt gatgtgtcca ggataatcca
    25981 gttcttcttc gattcgttta cgaatatcac gtactaaacg aacagcgtct aaatccgaga
    26041 tttcttctgg cttgaccatt acccgaactt cgcgtccagc ttgaactgcg taacttgatt
    26101 ctacaccggc gaagctgttc gaaatatttt ccagacttct taaacgacgg atgtagtttt
    26161 ctaatgattc acttcgcgca ccaggtcttg ctgctgagag cgcatctgct gcagctacaa
    26221 ggacagagat aacagaagta gcttccacat ctccatgatg agaagcgatc gcgttgatta
    26281 ctactgggtt ttctttgtac ttggcagcta attccgcacc gatctcaacg tgagatcctt
    26341 caatctcatg gtcaagtgct ttcccaatat catggagcaa cccggcacgt tttgctaatt
    26401 ggatatcttc gccaagctct gctgctaaga taccagctaa ttttgcaacc tcgaccgaat
    26461 gcttcaagac gttttgtcca taactagttc tgaatcgcag acgtccgatg atcttgatta
    26521 gatctggatg aagtgtatga gcaccgactt caaacgctgc ctcttccccg tattcgcgaa
    26581 tacgttcatc catttcttta cgagatttct cgaccatttc ttcgatacgc gctggatgga
    26641 ttcgtccatc ttgtatcagt ttttccaagg tcatccgtgc gatttctctt cggattggat
    26701 cgaatccgga aagaacaact gcttccggtg tatcatcgat gattaaatcg ataccagtca
    26761 gcgtctcaag cgttcgaata ttacgacctt ctcgtccaat gatacgacct ttcatttcat
    26821 cattcggaag ggtaacgact gaaacagtcg tttctgaaac ttgatctgct gcgcagcgtt
    26881 gaatcgctaa tgataataag tttttcgctt tgcgatctgc ttcttctttg gcacgctgtt
    26941 cagattcttt taccattaga gttaactcat gattcagctc ttcttcagtc gatttcatga
    27001 tgacatctcg tgcgtcctct tttgataaac cagcaatttt ttctagttct ttttgttgat
    27061 cttcaatcat tttttcaacg tctttttctc gctcttcaat taattgctgt tttgaactaa
    27121 gtgcttcttc tttactttca agtgagttct cgcgtttttc taacgagtca tctttacgat
    27181 tcagcgtttg ttcgcgttga atcaaacgat tctcttgaga ttttaattct aacttacttt
    27241 ctctcagctc actttcaact tcagaacggt atttctgatt ttcttcctta gcttctaaca
    27301 atgcttcttt ttttagagtt tctgcttctt tttttgcact atcgatgatt ccagcagctg
    27361 atgattgtgc accagctatt tctttctcgt gacgtgactt tgcgaccata aaccctaaac
    27421 caagaccgac aattaaaccg atgatagcga ggagaatgtt gaataccata aaatccacct
    27481 ccatactatc tttttcattt ttatcatcta tacagcttct atgataaatt attctaaact
    27541 cgtcaatatg catcttcgca tatatgtgta caaaaaacat gcacaacttt attctaatgt
    27601 ttgtaccccg tagtgtcaac tttaaataaa caaagaaacg gctttctact tattcattaa
    27661 acgatggttt ttaaaataaa tgacactagt ttcatatagg taaaccttta aattgagaaa
    27721 cttttctatt aaattattct ttgtagatga acagatattt ttattttatg tacccactcg
    27781 tataattaaa taactcacga tttctttatt caataaaaaa acggcaggtc aaggagagta
    27841 cctactcttc agaccagccg tttttgttta tcataatttt tattcttcta ctaaaggcaa
    27901 ttcttcttgt cctttttcat cttctgggac agcgacttct tcaccaatac cataagctgc
    27961 tctaactaga gcagaaactt ctgccatcat ttcaggatga ttagccatat agattttagc
    28021 gttttcccgt ccttgaccaa tccgatcttc tttataagaa taccaagcac cgcttttatc
    28081 aacaatgtct ttttctactg ccatgtctag aagttctcct tcttgtgaga tccctaaacc
    28141 atacatgaca tctacttcag cgattttgaa aggaggtgct actttgtttt ttacgacttt
    28201 gatctttgta cggttaccaa caatatctgt accttgtttc aattgttctg cacgacgtac
    28261 ttccaaacgg atcgttgcgt agaatttcaa tgcgcgtcct ccaggagtga tttctggatt
    28321 cccaaacatc acgcctactt tttcacggat ttggttgatg aaaatagcga ttgtttttgt
    28381 tttgttgatc gaaccagaga gtttacgcaa tgcttgagac atcaaacgtg cttgtaaccc
    28441 gacatgtgag tcacccattt cgccgtcgat ttctgctcgt ggaactaaag cagcaactga
    28501 atcgactact acgatatcta cagccccact tgatactaaa gcatcagcga tctctagacc
    28561 ttgttctcct gtgtcaggct gtgaaagaag tagttcatcg atattcacac ctaatttttg
    28621 tgcatattgc ggatctaacg catgctcagc atcaatgaaa gcggccgttc cgccattttt
    28681 ttgtacttct gcaatagcgt gtagtgcaac tgttgtttta cctgaacttt caggtccata
    28741 tacttcgatg atacgtccac gggggtaacc gccgacacct aaagcgacat ctaacgcaag
    28801 agagccactt ggaattgtag agatttgttg gtcgattttt tcgcccaatt tcataataga
    28861 gcctttacca tagctctttt caattttttt cagtgcagca tctaaggctg ctttacgatc
    28921 atctgccaat acgataatcc tccttatatt tacaaatcag agcaacattt cattatcctt
    28981 atcatatctt ttttagtgaa aaaaagcaag aaaaaaccga acaaatattc gcatttatca
    29041 ttttcttaaa agctgttttc tcaaaagatc aaatccttgc ataaccgcat tatatctgat
    29101 agcagcacga tctctggcaa agtgatattt ttccgaaacc acgccgtctt tagatgaaat
    29161 agcgatccat actgttcctg cagattgacc ttctaacgtg tccggtccag ccactccggt
    29221 gaaagctaca ctgtaatctg ttcttgctat ctcacgtgct tgaatggcca tctgctccac
    29281 acattctttg ctgactgttc catattctgc taatagctcc ggatcaatct ccagaaaacc
    29341 agcttttgtt tggagcgagt aggtaacgaa tccgcctgga aatacttcag atacaccagc
    29401 aatgtcgcct aatgcggctt ggaaggcgcc ggctgtcaaa ctttccgctg ctgttacagt
    29461 ctgcttgttt tctttcagta gttccactac gacttttgct agcgaattat catcgccgta
    29521 tccataaaaa tattccccta ctcgttcttg gattttttct tctaatgcta aaagagcttg
    29581 attccctgcc tgaacatcat ttgttttcac tgtcagtcgt aatgtcactt catttggttt
    29641 ggcatatggt gcgatcgttg gattgatctg tgtctcaatc agatctttta gctcagtgac
    29701 cagtcgcgat tcaccaatcc cataaaagcg caaaacttta gaaattagtt tttcttcaga
    29761 aggaaaacgt tcctctagta aaggacgtac ttgttccaca aacattggtt tcaactcatt
    29821 aggcggaccg ggaagaagga tatacgcatg tgtatctgtt tgataaaaag tccctaaagc
    29881 aagccctgta cgatttggta gaggaacccc gccttcgata atctgggact gttgcagatt
    29941 gtttttggtc atttttcgat gggttgtttc aaaatatgcc aataattttt tgtatccttc
    30001 cgtattttgt atgagagact taccgaggtg agccgcagtt gcttcttttg tcaggtcatc
    30061 ttctgtcggt cctaatccgc cacataagag tatgagttca cttcttgttt cagctagagc
    30121 aatcaactct tctaaacgct gctgattatc tcctacaact gtttgataat aaacttcgat
    30181 acctagatca gctagctgtt ctgacaagaa agtggcattc gtattcacga cttgtcctag
    30241 taaaagttct gttccgacag ctattatttc tgctttcatt acttcccgct cccttatata
    30301 gtatacgctg tatttttata tttttttatt taaaaagagg gcaaggagct gtgacaaaaa
    30361 tcttgtcaca gctccttgtt ccaaataaac agtgttctcc tgccaaaacc tcggtcataa
    30421 gtcacaatat acataaaaca tgaaaagctg ttttttgaaa ttatcccttg tgactcagag
    30481 ttagacggca ggctcacaac ctctccacta aacgatactt acattgatcc tttgaacaca
    30541 ccgctgtttt tgatgaagta atccacacct gaatagatag taaagatcag acaagcataa
    30601 agcatgattt gatctactgg aataccgatc aggctgaatg ggatattatt gataaacagc
    30661 aagataatag caaccatttg tgtagccgtc ttgacttttc ccggccaagc tgcagccatg
    30721 acttctccat gttcgacaag tagtaaacgc aagcctgtga cggctaattc acgacagaca
    30781 atgatcgcta ctacccaacc aggggccttt ccttggtcaa ccaacatgat aaaagcagtc
    30841 ataactagca ttttatctgc caacgggtcc gcaaacttgc caaaattagt gacaaggcct
    30901 cgcgcacggg caatttttcc atctaaccag tctgtgatac tagctaaagc aaaaataact
    30961 gcacctacta gctgtgtgac agccagtgtg gtgtctccta cgttaaattg tccccaatct
    31021 aaaggtaaac ctacaaccaa aatgaaaatc gggatcatac aaattctaag tacagttaat
    31081 ttatttggta aattcacact atcgactcct ttcttcccta ttctacgtga ctatcagcga
    31141 agtttccacc gatattattt acttttccaa atgaatttat tcaaaggagt atttttattg
    31201 aaattgatct ttttgaatta acgaagaagg accgcgacac agatttgtca cgatccttct
    31261 tttttatacc tacaagtaaa cagatgcgta aactaattta tacgatcatt ctgcgtattg
    31321 aagagtaagg ttcacatttt ttggactctg attgttttcc cctggattaa ctggtacgga
    31381 ttctccattc gctttgatcg ttgcattgct tgctgcacca atcgtgatgg tcgcatttgt
    31441 tgctgtttcc ggtaacacgg tactctgtgt ttcatttgct gccagtgtcc cttgataaac
    31501 ataggcattg tctactagca cacctaacca tacacgattg acagcagtaa attcaagggt
    31561 tacaggcttg gtagcgtctg tgatatttac tgcgatggca ctgccagaat cttctcctgt
    31621 agctattgcc attttctttt cttcacttga ttttgttgtt gattctactg tagaagactc
    31681 tttggtctgc gtgctttgct ctgaggaaga ggactcctcg actgatcctt ctactttaag
    31741 cgaagaagct gtgtctccga ttattggatc agcttgacga tcttgccaag tcatatagcc
    31801 cacgacaacg acaatcgcca aagcaaccaa tcctaataaa atgacaggaa gacttgtcca
    31861 aaagcgactt ggatgcgatt cttcctgatg cattgctttt ctagaagtac ttactgtttc
    31921 aggctgcgga cgttcaggga ccgatcctgt caatttgctt ttcccatcaa aaacatcgac
    31981 taaatggtct ccatcttctc ctacagcttc agcgtattgc cgaataaatg cccgcacgta
    32041 aaatttacca ggcaattggt caaaatcatc attgtcgatt gcttccaaat aacgtttttg
    32101 aatttttgta atctgctgta attcatctaa agatattttt ttattcaggc gtgcttgccg
    32161 taatttttct ccgatggttt caaaagccac tcgttcatct tctccagtct gtttgattct
    32221 ccgaagcatc tgctgttgca acgaaagacg ttatgtttgg gtgctgtgac aaaagtcatg
    32281 tcacagctcc ttttttccga ataaacattg ttctcctgcc aactcctcgg tcacaagtca
    32341 caatatccaa taaatataag aagctgtttt ttgaacttgt tccttgtgac tcggagttaa
    32401 acggcaggct cacgacctct ttcttaaacg gtgttcaatc agctgttcct cggtattagc
    32461 tcaaaaaccg gcaaaatata aggagatatt tttccgtatg tttctctaat actcaggtca
    32521 aaacgctgat ttcacaacct cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat
    32581 aagtccaacc aagttaaaaa cattaagaag ctgtttttac ttggctgttc ttattacttg
    32641 atgcagggca gctttttcac aacctcttgg tacacggtgt tccagaagta gcttcttcgg
    32701 caataagccg aaatatcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc
    32761 ttgaagctga ccacttctgt cacaacctct ttgattactt cgtttgattt tattttaata
    32821 taagttgatt aaaagagagc atgatttgtt ttattttctc actaattttt ccaacacaac
    32881 taagaatacc ataaaagctg attttggagg aggttcctac tacaagtcta aaacgaaatt
    32941 taagactttt cctcataaaa tactggttta ttttggtctc atgtaaaact cgctgaaggc
    33001 ctcttcactt ataaatgctt ccccagctgc cagtacgtct ttcatttgga tcgaatcgat
    33061 gatttcagga agatcaaata aagttcgatc gccaaataaa ctttgcgtaa attgattggc
    33121 aatatattcg atcgaattta atgattggaa atattgtcca agcattttct tcttcagcaa
    33181 atcgagattt gtttctgaca cttccggatc atcagcgaat ccaagaatga tttctttgac
    33241 tttttctgct gctttctctg gttcgtccgt atctccgctg aagtccgcaa agtgaaattc
    33301 acgatccaag ctgaattcaa aaccaaagct gtcatcgata attccttgat tgtacatcgc
    33361 tagatagttt cgagaagtat tgcctaaaat catttggaac aacagattga ttgctgtttt
    33421 atagcgtaac agttctgttc cttcttgcgg caatgtgtct aatcctttaa taccaagtac
    33481 aaatttatct cgtgtaatgg ctgcttccaa tgcactttgt ttgatgattt cttttgtgtt
    33541 ttcagggaaa taacgtacaa tttcttgctt tggagggaaa ttttttgctt cttggttttc
    33601 ccgaataagt ttcatcaatt tctcaggctc catcttacca actacaaata aaaccatatt
    33661 gcttggctga taaaacgtcc gatagcacgt atacaagtct tgagctgtga ttttgtcgat
    33721 actttcaacc gtgcccgcaa tatcgatatg caatgggtgt gtaggataca agttatttag
    33781 tatgccaaaa aacatccgcc agttagggtc gtcttcatac atttggattt cctgaccgat
    33841 gattcctttt tccttgttta cagtttcttc tgtgaagtaa ggtgcttgta caaaatcgat
    33901 cagagtcgtc agattctttt ctacctgatc cgtcgtagaa aacagatagc tggttttcgt
    33961 aaaacttgtg aaagcattgg cggaagctcc ttgtttccca aataattgaa agacatcacc
    34021 atcttctttt tcaaacaact tatgttctaa aaaatgggca attccatctg gaactttcac
    34081 cttttctttt tcaccatagg gaataaattc gttatcgatc gaaccataat tcgtactgaa
    34141 caaaccatat gtcttgtgat agtcattttt aggcaacaaa tacacggtca aaccgtttgg
    34201 cagtacttca tgataaagtg tttcattgat ttgctcatat tctgttttat tcatttgcat
    34261 ttcctcctgc taaaaagaag attgcctgca attcgatttg ctcagctact tgttgtatct
    34321 ctttgatcgt tacgctctcc atacgtttca accattcttc atcagataat tttgtttccg
    34381 gcagccagct gtccaaaaaa gaagcttcga ttgccgcttg cggagagtct agagaaagca
    34441 gatattggtt tctcagcatt gcttttgtct gagacagttc caactctgtt atttcaccat
    34501 ttcgcaaact ttctaattgc tcgtgaatca gatgaagtac ttgatttcgg ttcttttcat
    34561 cgataccggt ttggacactc atgaatcctc ggaacgtatc cacgctacta gaggcataat
    34621 aagctagact ttctttctct ctgacattca tgaaaagttt agagtgagga aaaccgccaa
    34681 ataaaccgtt aaagaccatt agcgcaaaac gttctggttc atcgtaatac acatttgtct
    34741 gataagctag gttcagcttt gcttgaacga ttggctcacg tacttgtcgt tccttcacga
    34801 tattcacttg aggttgggta taaaacagat ctggataaac tgtttcccgc gactcaaatg
    34861 gtaattgccc aactgcttca gctgctttgt ccggatcaac atcgccgaca acaaaaatgt
    34921 cgatttgatc atttgccatc atttcttgat agtaggacgc tagcgaacta gcagtgatct
    34981 tgtccaaatc ttcgactacg cccaaccctg ggatcttttg ttccgggctg ttttcaaaat
    35041 aaagttcttg caaggcatag gaagcaaaag tttgtttatc ttccttcaaa ctttccaaat
    35101 atagacgcag attgttcttt tccaagtcaa acgtcaactg atcaaactga tgatttttaa
    35161 tatttggata aaataaaacc tcttttaaaa aatctaccgc ttgagaaaaa aggtttggat
    35221 cattgacata ttcaccattc acaaaactga tccctgcgtt tagccaatgt aaatttcctt
    35281 tttttctaac tgataatcca aagcttgcgc cataaagctc tgcaagttgc gcacttaact
    35341 ttgtctgatc cggataattc aaactatttg tttccaacat gctgctgaga agtgaacgtt
    35401 tcgtgatgac ttcttgttgc agacgcgcag taaaacggat aaaaatccgg atcgttttgt
    35461 attgttttgt tggtaagaca tgaagattca cgccttctgc caattgatac accatattta
    35521 tatgctcctt ttcacagttg attgcccgct tactctttta atgggtcaac ctgccttttt
    35581 tacttattcc tctgcttgaa atttataagt tcaggcaatt cgccgttttt tctaaatgat
    35641 gttacacttt atagtatagc atagatgttt tttatcaaaa gaaacagacg gctaaaagcg
    35701 tatattgttt ttttattttt tatttttcga tatactaaag acaaataaaa gaagggaggg
    35761 tcattcatga tcaaagaatt caaagagttc atcatgcgtg gagatgtctt ggatcttgct
    35821 gtcggcgttg ttattggtag tgcgtttaca agcatcgtaa accaagtggt gcaaggcttg
    35881 atcacaccac taataggttg gtttgtcgca atgatcacag gtacttctga tttagaagga
    35941 gcactttcaa ttcttgactg gtcgccgaca aaaggcgtca catttgcgtt tggtaacgtt
    36001 attagtgcta tcattacatt tctgatcact ggttttgtct tattcttagt tgtcaaagcg
    36061 gcaaaccgtg cgaagaattt acgctccaaa gaagagcaag tggaagtgga agaaactcct
    36121 actgcagaag attatcttag tgacatacgc gatcttcttg ccaaacaagt ggcagaagac
    36181 aatacacatc cagtagagca aacaaatctt tccgatactt ctaaataata aagaatctaa
    36241 aaaaaagaaa gccgattttc agtgtcggct ttcttttttt agattcttaa cttgctactt
    36301 gttccttttt catgtcgata tgtggaatat catcttccaa atacacatct gagattgcct
    36361 tgaatccaaa cgactcataa aaagcttgta ggtattcttg tgcgccgatc acgattggtt
    36421 tttctggaaa atttttctgg atcatgttaa tcgtttcttg aacaatcttt cttcctaaac
    36481 catctttacg gttgtcctga tgaacaagga cacgtccaaa atggatactc tccgcttctt
    36541 catatactcg cgtgtaggca aggatcttcc catcagtatc ttgaaaaaac gtatgccaag
    36601 cttgtccatc aatgtcatca acttcttgat aaggacactg ttgttccaca acaaaaactg
    36661 cgattcgttc tttgacgatc gcgtaaaact ctgctgttga caattcttca aatttcttta
    36721 cctgatattc cactaagcaa acgccccctg cttttttatt cttagcatac cgattcgcac
    36781 acaaaaaagc aaacgaacct tacgaatctc aggctcgttt gctttttaaa ctgcttcagt
    36841 taaaaattcg atctcaactg tccgagtaag tacatctgaa tagctgtaag atactcgttc
    36901 aaaagaattt tcttcaggat ccaaatcaac aacgaatact gaaggatagg tttctgttaa
    36961 aacaccgcgg cgttctgttt gacgctttct tcctgtctga gcaaccaaca tgattgggct
    37021 gccgatacga ccttctaaat ctttcttgat tgaagctaaa gttgttggca tacagttcac
    37081 ctctactatc aactatacca catattttta aaattttcaa gtttaccatt ataaaaaaag
    37141 aaatgcaata aaaataaata agaagctcat taagattttt ctaaattatt cctcattata
    37201 aaatttcaag tgttttttta ttttgagttt tactctcccg taagcgctca ttacttgctt
    37261 gtgatccatt gctaaacatt cagccatgtc tacaaaatca acgccttgca tataataatg
    37321 aaaaatttta gcttctattt ttgaaaactg gatttcgcaa ttttcgattg cttcttttaa
    37381 taaaagatgc tgaatcgcta aaggttcttg caatgtgatc tctgctaaaa agtctggtcc
    37441 ttctgtctct attttttggt cgattgagac ttcatctttt tgatcacgac gcttcagcgc
    37501 gttctgcttc ctcaacagag aatagatttg attgcttata atgcttttat aataacttcc
    37561 aaagctaaca cctttgcttt tttcgtaagt tctcaatgcc tgatataaag cgatccttgc
    37621 ttcttgttcc caatcctcag aaacataatt tcttaaataa taggtgtttt tcaaacgtag
    37681 taaaagtgga aaatattttt gatacagggc agcaaatgct tcttcgtcac cattacgtgc
    37741 agcgcgcaat aactcctcag acataaaata attcctccat aaattcgttt atggaggaat
    37801 cttatcatca atgattcatc gaactgatta gaacgatatg ttatttattt tttctcatta
    37861 aatcgtccaa tttttgggac aattttttta actgctcttc attccatgga gaattacgtc
    37921 ggaaatcaat aaaatgcata tctttatttt cttgggctat tgttttttcc gtttttttta
    37981 tcgtcttata taattcataa gcagaagttc gtaatgctcc ttgggaaaag atcatccact
    38041 gttctgctaa atcgcttgtc gccactgtca cttgtgtcaa acggtcattc agctgaccag
    38101 cgattctctc gatataactg tcagccgttt cgtcttcttt cgtaaaaaca acatccaact
    38161 ggtatttctt ataattttgt tggatgcccg gaactagttg ggcatcgaag acaacgatga
    38221 cttccattcc ttcatatttc gcataatttg ataaacgatg taaaagagct tctcttgcat
    38281 ccgccatttt gtcctgcttt ttaagctgga caagttccgg ccacgaacca atcatgttgt
    38341 agccgtctac aatcaacagc tgttttttca tggtctactc cccctgacgt tgggaaaaag
    38401 cctgatacat aagcaaacca gcagcaacac tggcatttaa gctctggacg tgcccggtca
    38461 tcgggatcgt cagcaattca tccatttctt ttttcaatcc tggactcatt cctttgcctt
    38521 cattaccaat gactaaagca attgatcctt tggcattcca tttttggaag tttgtgccgg
    38581 tcatatctgt gccaaatacc caaaatcctg tttctttgag ctgttgcaca gcttgtacca
    38641 aattcgtcac gcgtgcaaca gggacatgtt cgactgcccc agtggaagtc tttgttacga
    38701 taggtgtgat tccaactgct cggtgttttg gtatgatcac accatcgacg ccacttgcgt
    38761 ctgctgtccg taaaatcgac ccaaaattgt gtggatcttc gatgctatcc aaaatcaaaa
    38821 agaaggggtc ttcttttttc tcttttgttc gttcaatcag ttggtcaagg gataaatatt
    38881 cataaggtgt aatcgcaaga acgactcctt gatgcacgcc gtgatcgctc aaagtttcta
    38941 atttttgttt aggaacccat ttgactggca cagcttgttc acgtgcgatt tctttcagtt
    39001 cttctacttt ttctcctcgt gaatcttctt gaagaaagag tttattccct cgcccttgtt
    39061 gtagcgcttc tactgcggca tggtgcccaa agacgaaatt atcttctaat gcttcttcat
    39121 tttgatcaga aactgactga tttcgtccag tttgcttttt atttgaatat ctattttgtt
    39181 ttttgtcttc aaatccacgt ttcgattgat gtttgtttgt tctttcttta cccatttttt
    39241 tcaccaactt tctgaataca ccagcggatc aattcttcca tccgctcggt ttgttttgtc
    39301 aaatgcagat agcccatgag cgcttcgaac cctgttgcga cccgatacgt cgtaacatca
    39361 gcattttttg cgctcgtatg gcttttagca tttcttcctc tgcgataata aagttcttct
    39421 tctgtcaaaa gatgttcttc cagcatttgc tgaatcaaaa aggcttgtgc attcgcagaa
    39481 acataattcg ttgccgcacg atgcagctta ttgggttttg tttctccttg atcaatcagg
    39541 tggtcacgaa catagatttc gtaaatcgca tctccaacat aagccaaggc taaaccgttc
    39601 aattgtgtgt aatctctcat gcttctcttc tccatcgggt tccttgcggt gtatcttcta
    39661 ataagatccc tttttctttc aacaaatcac gaatttcgtc gcttcggaca aaatcacgat
    39721 tgcttcttgc ttgatttcgt tcttcgatca attgttcaat ctcttcatct aataactctt
    39781 ctgctgtaaa taaaataccg aagatcgaaa gccattgcga gaataaagct tctccttgtg
    39841 ccaagacagc ggcagaaact ttcggctggt ctagataagt gttcagccac ttagcaagct
    39901 catagaccac tgtgatccca tttgctgcat taaaatcatc gtccatttct tcgatgaatc
    39961 gctgttctaa attttctaac aaagcgattt tatccgcatc atctgccaaa ttctctgagg
    40021 cagtttcttg gcggaatttc aaattgtcaa acgtattctt cagtcgttgt aaattggtgg
    40081 ctgcttcttt catcgtcgtt tcactgtagc ggatcggacg acggtattgt gtggtcgcca
    40141 tgaagaaacg caagacttgt ggatcaacgt ttttgatcat ttcatgtaca gtgatgaaat
    40201 tccctagaga tttactcatt ttttcatcgt cttcgccaat cgttacatag ccattgtgca
    40261 tccagtaatt agcgaatttt ttccctgttt tcgcttcact ttgtgcgatt tcattttcat
    40321 gatgaggaaa ttctaaatct tgcccgccac catggatatc gatcgtttct cctaaatgtt
    40381 ttgttgccat aacggaacat tcaatatgcc aaccgggacg tcctttgccc catggtgaat
    40441 cccaagaaat ctcgccaggt tttgcttgtt tccataaagc gaaatctaat gggtcttctt
    40501 tttgctgctg ttcttcccct gtacgctgac ttgctccgac ttccagttca tcgatggatt
    40561 ggtcacttaa tttcccataa ttttcaaatt tacgggtacg gtaatacaca tcacctgaag
    40621 attcataagc atagcctttt tcaaccaaca cttgaataaa tgctaaaata tctggcatat
    40681 gatccatgac tcttggatgc agtgtagcag gctcgacatt caacgctttc gtatcttctt
    40741 caaatgctcg gatgaaacga tcagcaactt ctggtgctgt aatacctaat tcgtttgctg
    40801 tacggatgat tttatcgtct acatccgtga aattcgatac gaaatcgact ttatagcctc
    40861 gatattcaaa atagcgtcga attgtatcga aagcaatggt gctacgagca tttccgatat
    40921 ggatataatt atacacagtc ggtccgcaga catacatacc gacatgtccc ttttgtatag
    40981 ggatgaattc ttcttttttt ctggtcaatg tattgtagat ttttatcatt ctgatctcct
    41041 cactttttct cctttgattc gaacgacttt tgctggtact cctaccgctg tagcgtcgtc
    41101 tggaacatct ttcaacacaa cggcacctgc accgattttg gcgttttttc cgatcgttac
    41161 cggtcctaat atctgcgcat tcgcagagag catggctcct tgtttaacag ttgggtgtct
    41221 tttacccgtt tcttttcctg tgccgcctaa tgttacgcca tggaataaaa tcacatcgtc
    41281 ctcgatttct gcggtttcgc caatgacgat tcccattcca tggtcgatga acacgccagt
    41341 tccaatcgtt gctcctggat ggatctcaac accagttagg aaacgccaga attgagcatt
    41401 gatcttcgcc aataaaaaga gccggtgctg atataaaaaa tgagagaaac gatgccaaaa
    41461 caaggcatga aaaccagggt atgtcaaaac gatctcagct gtcgaacgtg cagccggatc
    41521 gtttctcttt gctgcatcaa tcgctcgggt aaaccaactc atgactttcc actcctttcg
    41581 taagaatttt atgtaggaaa tcatcttctt ataaagaaaa gaagcgcctt ttaaataagt
    41641 ttccttatca aaagacgctt tccgcgtggt tccacttttc ttcataatca atgattatct
    41701 ctgacagcta acgcctgtta tacgttcttg gctactttgt ttcctataaa acagtttttc
    41761 ctgttttctg tgtttcgcca agaccactcc cagaggcatt tcggcaacca ttcattgata
    41821 ccttgcacca cccggtatct ctcttgtaat gatcagctgc ttactttttt ctgttcatcg
    41881 tgtttagttc agtacttgat ttaaatgtgc taatgatttt tcacgaccaa gcaattcgat
    41941 cgtttcaggc aattctggac catgcatttg tcctgataca gctacacgga ttggcatgaa
    42001 aagatttttt ccttttacgc cagtttcttt ttgaacagct ttgatggcag ctttgacagt
    42061 tggagcatct actacttcca tttgttctag ctgtgctttg aatgcgtgga gaacagtagg
    42121 aactgtttca ccagcaagta cttcttttgc tgcttcattc aatacaggat gttcattgaa
    42181 aaatagcgta gacagatcaa cgatttctgc agcatagctc atttgaggtt gatacaggct
    42241 aacgatcttc ttcacccatt cgattgtctt ttcatctggg ttttctgcta cacgtccatc
    42301 agcgattaga taaggcagac acatgtcagt aaatgcatct agatccattg ctttgatgta
    42361 atggttgttt acccattgca gttttttcgc atcgaaagct gcgggtgatt tacttaggcg
    42421 ttttggatcg aacattttga tcaattcttg gtgttcgaag atttcgtctt cgcccacagg
    42481 tgaccagccc aataatgcaa taaagttgaa cattgcatct ggtagatagc ctaattcgcg
    42541 atattgttcg ataaattgca agattgattc gtcacgtttg cttagttttt tacctgtttc
    42601 actgttgatg atcaatgtca tatgtccgaa ggttggtggt gtccagccaa atgcttcata
    42661 gatcatcagt tgttttggtg tattggcaat atggtcatct ccacgtaaaa cgtgagtgat
    42721 tttcatcaaa tgatcatcga ccgcaacagc aaaattataa gttggcatac catcgcgttt
    42781 ttgtatgaca aagtcgccac cgatattatc ggattcgaac gaaatagcgc ctttcaccat
    42841 atctgtaaat gcgtattctg tattgcgagg cacacggaaa cgtacaacag attctagacc
    42901 ttgtgcttct ttttctgctt gttcttcagg tgtcaagttc gcacatgttc ctgcgtaatg
    42961 aggcatttct cctcttgcgc gttgtgcttc ccgttctgct tctaattctt cttctgtgca
    43021 atagcatttg tatgctcgat tgctagcaag cagctgatca atcagcggtt gatagatttc
    43081 tttacgttca gattgacggt aagggccgta ttctccagga ttttcaggag attcatccca
    43141 attcattccc aaccaagcaa ggttttccaa ctggcttttt tcgccatctt cgatattacg
    43201 tttttggtca gtatcctcaa tacgaataat aaagtctcca tcattgtggc gggcaaacaa
    43261 ataattgaat aatgcagttc ttgcatttcc aatatgcagg tgcccagttg gacttggtgc
    43321 atagcgtacg cgtactttcg tcattttctt cctcttcttt caaactagac tttgactaac
    43381 agcaaaaatt ccgtcagtta cggcttctca aatatcattt atttaaagcc actcagcaca
    43441 aattttacct tttctaaggt aaatagtaaa gccaactggt tgaatttcct taccagttgg
    43501 ctgaatttct tttttatttt gattgtttag tctttttctc tgtctctttt ttattgtcga
    43561 ttcctcgtcc tgcatgtgca ggtttagcaa agatcatgcg tccagcagct gtttgaaggg
    43621 cacttgttac aacgacttcg atacgttcat tcatgtaatg ctgtccatct tctacgacaa
    43681 ccatcgtgcc gtcatcaagg taggctactc cttgttggcg ttctgtcccg gcttttacta
    43741 ctagaacatt catcgtttca ccagggatga cgactggttt tacggcattt gccaacgcat
    43801 tgatattaag aacaggtaca ttttggaatt cagagacttt attcaagtta tagtcattgg
    43861 ttacaatgac accatctaaa agcttagcca atttgatcaa tttgctgtct acttcactga
    43921 tatcttcaaa gtctccatcg tacatttcga cagatatacc ctcttctttt tgcaaggcat
    43981 ttaggatatc taatccgcga cgaccacgca cacgtttcaa actatcccct gagtccgcga
    44041 tgtattgtag ttcgtaaaga acaaaattag ggatcatcaa ggttccttct aagaatccag
    44101 tctttgtaat atcataaatc cgaccgtcaa tgatcacgct tgtatccaag attttatatt
    44161 tatggaaatt ttctcctgat tttcgttcta acagttgact ctcaacttga ctagctgttt
    44221 cttgctcgat ttttttgctt cgtgaaccaa aaactttctt ccattcttcg attcgtgtcg
    44281 ttcccattct caatcccaga tagcctaaga tgatcatcag taaaatcggc aatacactat
    44341 tgacgaatgg aatttccata ttgtaaagag gggtcgagat cacaacacct aaagtcaatc
    44401 cgatgatcgc accgatcgca ccgaagagaa gataagtcaa gctgacctca cttagttttt
    44461 gttctacttg tttgataata gctgagatat gtttggccaa cagcaaagat aataaaaaga
    44521 aaataagtgc gcctaacagg ctgttggtaa atgtattatt cagccatccg tttgactgtt
    44581 gaccgattgc ctgccaagct gagggtaaaa atgaaatacc caagcttgcg ccgacaatga
    44641 tcatcagcaa agtaatgaca cgtttttgca tcgtttttcc tccgtttcga caatcattta
    44701 ttagttagcg gaacacacgc ttcaacgttt cagccaaagt tgctactcca actatctcga
    44761 ttccttttgg cgcttcccag ttacctaagt tgtttttagg taggtatact tttgtgaagc
    44821 cgagtttttg gacttctttg actcgttgtt caatatgact gacacgtctg atttctccag
    44881 tcagtccaat ttcaccgatg aaacattccg ttggctgtgt tcctttttct ttatagcttg
    44941 aagcaatact gacagcgata gctaaatcaa tcgctggttc atttaatttt accccacctg
    45001 cagcttttaa gtaagcatct tgattctgaa gaagcaaccc agctcttttt tccagcactg
    45061 ccatgatcag agatacccga ttgaaatcaa gacctgttgt tgttcgtttg gcatttccga
    45121 acatagtagg tgtgactaat gcttggatct ctaccaaaat tggacgtgtc ccttccattg
    45181 ccacaacgat agcagagcca gttgcaccgt ctaggcgttc ttctaagaaa atctgtgaag
    45241 gattagcgac ttcttccaat ccatgctcat gcatttcaaa aatcccaatc tcattcgttg
    45301 agccaaatcg gttttttact gctcgcaaaa tccgaaaact atgatgttgt tcaccttcaa
    45361 aatataacac cgtatccacc atatgttcta acattcttgg gccggcgatc gagccttctt
    45421 tggtaacatg accgacgata aaaatggcta tcccatttgt tttagcgatt ttgagtagtt
    45481 ctgcagtggt ttctcttact tggctgacac ttcctgccac acttgtgatg tctggctgcg
    45541 tcatcgtttg gatggaatca atgataacat aatcagggga gatatgttcg atggcacgac
    45601 tgatttcgtt catatctgtt tcagcgtata gataaaattc tgtgttgata cttccgaggc
    45661 gttccgcgcg taattttatc tgctctgcgc tttcttcgcc ggatacatat aagacttttc
    45721 cgcctatggc tgctaattgc tgagaaactt gtaacagcaa agtggatttc ccgatacctg
    45781 gatcgccacc aatcaagacc atagaccctg gcaccacacc tccgcctaac acacggttca
    45841 gttcttctaa ttttgttttg atccgaggtt cttttttagg gacgacgtct gcgatttttg
    45901 ttggttttgc tttttctcct gtcaaacttg tccgagttct gcggtccgtt gtatcttgtt
    45961 cgatttcttc gaccatcgta ttccattttc cgcaatttgg gcagcgtccc aagaacttag
    46021 gcgacacata cccgcaattt tggcaaacaa attgtacttt tgattttttt gccattgtac
    46081 cgcctccttt tatcctaatc atgagacatt aaatcaagct taagtatctt taacgtttta
    46141 tttactggaa gaaccaaatc cgccttgacg ttgagcagtc gtttgatctt gatctgcttt
    46201 taaaaacggc aaaaagatcc cttggccgat acgctcgcct tttttgatca ctacatcctt
    46261 gaaaccaaag ttggtgaatt ggaacatgat atggccttca tttgcctcat tattgtaata
    46321 atcactgtca atcacaccta ctccgtttgt caacactaaa aaccgcttca acggattgct
    46381 tgaacggtta gcaagctgca aaaattcatc ttctcccatg taagctttga ttccagtagg
    46441 cacgagggtt ggtttcaatt gtttttcatt ttctgataca tcttttttca atccatctgc
    46501 gactgtttcc aacccctttt tccagcctgc tttccagata cttggaatca cgacctcttc
    46561 tgctgcttca aaatcatagc cagcagcatg atgtgtagcg cgctgcggta gattgatatt
    46621 ttgatcttta tatttagaaa taacttcaaa tcctcttttt ctcaccggtt ttcctcccta
    46681 aataaactac tcctattcta acatatccga ggctgtaaaa gactttcttt acaaattctt
    46741 caagaaattc tgctatctta aagtaaacca ttaattcgtt ctttttccaa acgactgcaa
    46801 gttgtacaaa aaaactattt tttacatgac tttactcgtc aaaacaagta gaatagaacg
    46861 aaattaatga gtggatattt gattgaggtg tcacatgcgt aaagaaatta aaaatgcaaa
    46921 acgaattatt gtcaaagtag gaacaagtac attgatttat cccaatggaa atatcaatct
    46981 tgcggcaatc gatcagttag cttttgtcct ttctgatctt agaaatcggg gaaaggaagt
    47041 gatcttagtc tcatcaggag ctgtaggcgt tggtatgcat aaattacatt tgtcagaacg
    47101 tccgactttg attcctgaac agcaagctgc ggcggctgtt ggtcaagccg aactgatgaa
    47161 tatctataat caaagattca gtgtatataa tcaacagacg gcacagatcc tcgtgacaag
    47221 agacgtggta gattatcctg aaagtcgtaa aaatgtgagg aatacattgg aacagctttt
    47281 gattatggac acgatcccta tcgtgaacga aaacgatact gtagcagtgg atgaattaga
    47341 tcatctgaca aaatttggcg ataatgatca gctgtcagcc atcatggcaa aacttgtcgc
    47401 ggcagacctt ttgatcgtcc tttctgatat cgacgggttt tattcagata atccgactac
    47461 aaatcccgag gctcagatgt atcgctatat cgataaaatc gatgaaaaga ttctggcaca
    47521 agcaggtggt gctggtagca catttggaac aggcggtatg cagagtaaat taaaagccgc
    47581 ttctaggata tttgaagcaa acagcgcaat gattttagca aatggaaaaa atccggcaat
    47641 tatttttgat attcttgcag gaaaagaagt aggtacttta tttaaggagg acttgaaatg
    47701 actgacttga tgcaattagg acaacaggca aaagatagcg cgcaacaact agcacttatg
    47761 cctacaaacc gcaaaaatga gctacttgtt caaatggcac aaaccatcaa aaagaatcaa
    47821 caagcgatca ttgaagcaaa tcataaagac ttagaaaaag cagtcgaaaa caatatcagt
    47881 gaaacgatgc aagaccgctt gcagctgaca cctgagcgaa tcaatgccat ggctacagag
    47941 atcgaaaaaa ttgcttcgtt agacgatcct attggaaaag tagatgagat gtggacgaac
    48001 accgacggat tgagaatcgg caaaaagcgc gtgccactag gcgtgaccgg catcatttac
    48061 gaatctcgtc caaatgtcac gacagatgca gctagtctag cattcaaatc agggaatgcc
    48121 gttatccttc gcggtggcaa agaagcattc ttctcgaatc aattattagt ccaattattg
    48181 cagcaagtac tggtcagtgc tggcgaatct ccttatgcca tccagtttgt cgatgatact
    48241 tctcatgaaa ctgcaggaca gttgatgcga ttgacagaat acttagatgt gctgattcct
    48301 cgaggcggag caaaattaat ccaacgagtc aaagaacagg caacggtacc tatcattgaa
    48361 acaggtacag gaaataatca cgtatatatc gataaagaag cacagttatc tatggcagtg
    48421 aacatcatcg tcaatgcaaa agcttcccgt ccatctgtct gtaacgcggc agaaacattg
    48481 ctgatccatt ctgagatcgc accttttttc ttgccagcta ttgaaaaaga actggtagaa
    48541 cacggggtct ctttacgtgc ggatgctcgt gcgttagaat atctagagac ggctgcttta
    48601 gcagaagaca cagattggga tactgaatat ctagattata tccttgctgt caaagtggtc
    48661 gattctttgg atgaagcaat tgcacatatc aatcggcata atactaaaca ttcagaaacg
    48721 atcgtgacag atagttatgc ggctagtcaa cgtttcttaa atgaagtgga cgcggcggct
    48781 gtctatgtca atgcctccac tcgttttaca gacggatcgg tatttggttt tggtgcagag
    48841 attggtatct ctactcaaaa gttgcatgcc cgtggtccaa tggggttaaa tgaattaact
    48901 tccacaaaat atatcattta tggcgatgga caaatccgcg aataatgttt cgtgtgaaac
    48961 attattcccg ccttttgcct tttttctgta aaggttctat ccaactagaa aggtgtcaat
    49021 atgtttgaaa ttttaatgat tttttttatt taccttattt ctctgaatat cgctgctttt
    49081 ctgggagtcg gtattttatc ccttttcttt caattcaaaa aacgaagcat agggagtcaa
    49141 cgggaaaaat ggtcgcagta ttttgacaaa atcgggcaaa aagggctggt tacacgcttg
    49201 cacattagtt acatggttgc attatgctta ttagcgacaa tcaattacta ttcttttttc
    49261 gatcattcaa tcgcttatac gattacgcta ctgattgctg ggatttttca cttatcttat
    49321 aaatatcagc tgaataaaaa ccacttgaat cggactttcc gctaaaaaat atccgtacga
    49381 taagtagagt gccttgaaga tcggcaaaaa actgccggac tttttagagg ctgtgacaaa
    49441 gattttgtca cagcctcttt ttccatataa acggcgttcg cctaccagct gcctggtaaa
    49501 tctgtttcct cttcgttaag aagcgaaaat ccgtcaaatg tttcgccatc ataataacga
    49561 agcagctcat ccatggacaa gttcatcgtt ttttcttttt cttcttccgc tgattcagcc
    49621 agtagctgtt cccgatacac ctgatacata ccagctattt tttgctgcat cgcttttcta
    49681 gtttcacctt ccgcattgat tattggcaat tctttgatat agccacgata aatcaaccga
    49741 tcatctgaaa attcaatggg taattcaacg agtgtcaacc cgacttcctg aaaatattct
    49801 tccagcatct attatccccc cattcttttt cttaaatgaa ttttagctta cctactgaaa
    49861 attttccagt ttcattattt tgagaaaaaa agggaatgtg acaaaaatcg tgtcacatcc
    49921 cctttttccg aataaatggt gttcttctgc caactcctca atcataagtc acaatattca
    49981 acaaacatga gaaactgttt tttgaaattg ttccttgtga ttcagagtta aacgacagtc
    50041 tcacaacctc ttggtaaacg gcgttcaaaa gcttgcatct gcggtaataa gcccaaccaa
    50101 acgaaaaata tgagaagcta tttttgtttg gctgttctta tgacttgatg caggacagct
    50161 ttttcacaac ctctgatttc ttattcttct tcatttgtag caaaataatt acggtgataa
    50221 atgatacaac atacactgat aagaacaatg gcacctgcaa aataaaacgc atagctttca
    50281 ccaatatgtt caccaatcac acctgcaata aatggtgcta aagctccacc gaaaaagcgg
    50341 acaaaactat agctggacga agcaacgttt cgttgcagac ctggtatttc catagcaatc
    50401 gtcgtcaaca gtgtattgat aattccttgg aaaaatcctg ctaacacaat gctgatcggt
    50461 acaaggactg gcatagaagt tccagctcct aataacagca aacaaataat gaataagaca
    50521 aatgctgcta aaattgtccg ataagtcgta aacttccgct caagagccgg tgcaacgaaa
    50581 atcgaagcaa tagccagaag cacaccccag ccaaaaaata caaaaccgac ttctaattca
    50641 gaatagccaa tcaataaaaa tggcgcatac gcaagtaaag taaagaatcc gaaattatat
    50701 aacaatgcga ttagtccaac tgaacgaagt ttatgattgc ttaacgcttt gacaccttcc
    50761 caaaggccta cttttctctt tggtttctca gtttcaggta aaagtaccgc tagggctata
    50821 aaaccgcaaa acatcaaaaa tgctacccca aaaaatggat aacgccatga accactgccc
    50881 aagaccccac cgactagagg gccaacagcc attccgcagc caagtgctgc ttcatacatc
    50941 atgattgctt tttctgtgtt accaactaaa atactgacga tcgttgctaa agcagtagag
    51001 acaaataaag cattcccgac accccagccg gcacgtaacc caatcagcat tccgatattc
    51061 ccagataatc cgccaagacc agcgaataca acgatgataa acaatcctgt catcaatgtt
    51121 ttttcaaacc gatccgacta gataagaaac ctgtaaatag catcaccaca ccagtgacta
    51181 acatgtaact agtaaataac agggtcgttt cagctggcgt agcgtttaac tgtgcagcaa
    51241 tcgtttttaa gattgggtct actagaccca ctcccataaa agcgatcata caggtagctg
    51301 ctacaactag cgctgaactt ttcccctttg tttcttcatt tgtttgttct tgtatatcta
    51361 tttgattctc caatatttcc atccctttct ctctattagc atcatacatg atttatttta
    51421 catgtaaata tgcacatgta aaacgtggaa actttactgt gatataataa agaaaataag
    51481 acgagaggta ggatttttat gaatacttta gcctatatat tattatgcgt gcttttatcc
    51541 aaaccacgtt ctgggtatga attgaagcag ctgatcacaa ttttttggga agcccatcac
    51601 agtcagatct atacaacgct tgccaagtta gaccagcagg gttatgtgac cgttttggac
    51661 aaagacgagc actctcaaaa aaagatctac cacttgactg atgaaggacg tgctcttgta
    51721 gaggaatgga tcaaagagga aactcccgct cctacgcaaa aagatgaatt tctagcaaaa
    51781 atctatgctt tttcgacgct cgataaaaca acagctactg gactgctatt cactagagaa
    51841 caacagctga acaaaatcat gcaaaaaaat caaaaaaaat tgaatgacct ctcgctagac
    51901 aagaaacaag aatttggacg ttatgttgtg atccagcgca gaattatgct ttgtcagcag
    51961 gaactccaat ggtgtcagca agtaagagaa caaatgcagc agttttttga gtaagggata
    52021 aacagaacct ttctagaaat atctattgaa gagcaaatta tcttcaataa aagagcaata
    52081 cagttggaaa agaaaaagcg atccgaacga caggccaaaa cctatcgttc ggatcactat
    52141 ttttctagaa ctgcattcaa gagactatga caaaaagcat gccaaggcct tttttcaaat
    52201 aacagtgttc catcattcat tactttgaat tgcttttagc acacttcttg aaagcgggcc
    52261 aacgatgatc aactgcaacg gtaaagcggc aatcacattc attttccaag cattcatata
    52321 agcagagaat aattgatcag gtattccttg ttccatgatc acaccgaata aagacataca
    52381 tgtcaccatt cctaaaacca tcaagcatga aatagttaag atcatttgga ccttgctttc
    52441 tggatttact ggtagtttaa aggcaatttt tttcgccagc acaccgatga tgaagacatc
    52501 caaaatgaaa gccaccacga atccaggcac tagaccaatc aacaaacttg ataaatgaag
    52561 atcttgatgc agccataaat tatacgcgct catccccaag accatcaaaa aacacatcat
    52621 tgttgtaaaa attattcctt cttttttatt tgtaggcaat ctgtaccact cctttaaatt
    52681 tgtcacgaaa atcatcgtag cacatttaaa aatttctcgt aagtactttt tttattttct
    52741 gcccttttat ttcataaact tcatcagccg tacgctcgac aaagtaagaa tcatgagaag
    52801 taaacaaaat cgttcccgga tatatttgca ttagcttctc taaagcttcg atcgttgcca
    52861 tatcaataaa attagtcggt tcatctaaca gcaacacatt acttggctta gtaaaaagca
    52921 aagcaatcgt cagacgggtc gcttctcccc cacttaatgt acataacggt ttcgtcaaag
    52981 tttcctgagc aaaccctaag tgattcagca aactacggac aagcgattct gaaaactctg
    53041 tttgtctcat gacaaaacga atgatggatt cttcagatgt catttgataa cccttttgct
    53101 gatatacttg aaagcttact ttcggagaga gaaggattcc ttctccttgt ttgcggatat
    53161 gttctaataa ggttgtctta cctgaaccat tttcaccgac aatcgcgatt tttttaccta
    53221 aagaaaactg aaaatcacca tttaccagta atgttctccc acttctttcc aattggacat
    53281 tttgtcccat gatgggatag cggctgtgga gttcaagaga ttttggtact ggaaaacgga
    53341 tttgtttcat ctgctgcggc ttggttgttt ctcctatttt ttggagtctt ctctctaatg
    53401 ttttcgcatt cttttggatg gctttttgta cactgtcttt ttgtttagag gaagacaacc
    53461 gatccggtct gatttgttgc tttttctttt tactggacac tttttccaac ttttccgctt
    53521 gttttttctt ttcttggatc gatttttcca atcgtttttt ctctttcatg atcgactctg
    53581 cttctcgtag ctgagtcttt ttctccaatt ccttttgagt gagataggca ctataattcc
    53641 ccgaaaactc tcggatttct ccgtctttta cttcccagat tttcgatgct agctcatcta
    53701 gaaaatagcg atcatgacta acaaaaacca gcgtaccata ataataacga agctcttcaa
    53761 tcagtcgatc aatacttttt ctatctagat gggttgttgg ctcgtctaga agcatccctt
    53821 gttcataaga agaaagaaga cgtatcaact cgatctttcg ttcttcccct ccgctccatt
    53881 tccgctctga agtttcttcc agtcttaact ggaaatgact ggctaattcc aaatttaggt
    53941 cctttgcttc agcgagatag gttaattgag gcaggtagtt gaaggtgatc tctgtttgga
    54001 ttttcccatg atcgggaaaa agttcaccgg ctattagttt gagcaaagtc gatttaccga
    54061 caccattttc tcctatgatc cctatacgat cttgctgata gacacgaaga gaatcaatag
    54121 atagttctag ctggttgcca aagctgactt gtagatttgt tatatttact gctaaatttt
    54181 ccatatgttt tacactccta tttcatgtaa aacaacaatt atcgatacta aaaaagagca
    54241 gaaaaatctg tttgcaaaaa aactgcatca catcttttcc tactctgaac gaaataacta
    54301 tgactaaatc accaaatctg tcatagcttt cacctattct actgtcgttc gaaaaaatgg
    54361 acatactcca tccatgaaat tgttgtttat acatgtcttt tgaatttatt tagttgttat
    54421 tcaaaagttc gaaacgtaat ttcatcgatg cagtcatttt tccgacctcc ttttttatta
    54481 ggttaccaca cacgattcaa aaaaagcaat agctactgaa tagtaactga aaacctttta
    54541 ttcgaacgta gtgatctttt ctggtgctaa acgaacagac tcgtaacctt cttctaccgc
    54601 ttttcctatt gataaaataa taactggaac ataacggtct ttatccaaac caaatgcctc
    54661 tgccaactga tctgcttcga atccaccaat tggatttgtt tcatagccat gatcacgcgc
    54721 aactaacatg aattgcatag ccgctaagct agaatcgact ttgactacat cgttcatttc
    54781 ttctcttgaa aaactcttgt aataaggaat gatcgcagct aattgttgat ctctgacttc
    54841 ttttggcatc ttccctgatt cgtaagcttg gttatagatt tcttcgccat attcgtaaca
    54901 ttgcatatcg ccaaaaatca tgaccattgc agaagaagta tcattttgtc tcgtattgaa
    54961 acggatcaaa ggacgcaaga tttcttttgc ttcctcgctt tcagcgacca caaagcgcca
    55021 tggttgcata ttgacagaag aaggtgcagt cgtcgcttct ttgatcattt ccagcatttc
    55081 ttcgtgtggg attttgtatg tttcatcata tacgcgaacg gatcttctac cagtcataat
    55141 tgttgaaaaa tcattttttt gttccattat tattaacctc tttcgtttct gttgttatat
    55201 tgcaaagttg ggtattcaat ttatctaaaa gtgttagaaa ttgtttcatt tctacatcgc
    55261 tgaaagccgg ggatagcacg gaacctgtat ctttatggtc catttcacaa tgctcgattt
    55321 tcttctgggc tttttctgtt agcgctacat acacttcccg gttgttctga ggatttctct
    55381 gacgcgaaac atagccattt gcttccaata ccttcagatg acgagtaact gctccttgat
    55441 caatctgcaa cttttcttgc aaactgcttt gtagcaatgg attttcttct ttcagtctca
    55501 tcatgatctc gtatctagta agacttatac cgatattttc ttcaaagatc tggacgattt
    55561 tttgatcggc taatttgatt tgatataaaa ggtgactaat ctcttctaaa gacataattt
    55621 cctcctttct aataattgac tcgtcaacga ttgacttgtc aataatagaa taaaaaaaga
    55681 aaaaagtcaa agaatcagct gtgtaataaa aaaaacgctc tcaatagaaa agtatagaga
    55741 aaagaaattt tttgtagaat gaacggaaaa aatgataaag aaagcacaac gctaaaacca
    55801 agattattga ttcttatcaa actatagaag atagctcttc tgcagtagaa aattttacac
    55861 cgccaaaaat ttccgaaatc gccttttgca agaatcccgt cgaatattag aaattggatc
    55921 agtatgctta aacagtaata aaaaagttat gacaagatgt tgtcacaacc ttttttgagt
    55981 aaacggtgtt caaaaacttg catctgcggt aataagttca accaaacaaa aaacatgaaa
    56041 atctgttttt gtttggctgt tcttactact tgatgcaggg cagctttttc actacctctt
    56101 tttaataacc ataaaatcct attccttcat atctccagcc catcggtgtt agccaatctc
    56161 tttccttagc actcaaggta tagtgatggg taccagtttt ttcatttgga ttaaatagtc
    56221 gataaattgg cttggaacca ccagaataaa aacttagacc ttcataacgc caaccgattt
    56281 ttgttaaaga gttgatctca gattgtgcca ttgtgtaatg atggtctcca ctgtaaggat
    56341 tgtataaacg gtagacaggt tgtccgctgt ttggtgcttg ccaagcaatc ccttcatatt
    56401 cccagcctac agttggcaga taattttttt ctccggcatt caatgtatat aaatgttctc
    56461 ctgtatgacg gttatacaga cgatacatcg gagaagttga agttggtgct gtatcatact
    56521 gtgtcagatt ataggtttcg attacattgt tcaatgtaga accataatta ggcgcagtgg
    56581 cataacgacc tgttagccaa gccgtagcat ctttgtaaga gttcgtattg cttttccatg
    56641 caccggaata gtagtaaacg cctggctgaa aagaagtcgt cttcaatacc gttacgttat
    56701 cttgcaatga ttctttgtac gaaggatatt ttctaaaagc ggcattcacc gtaatccatt
    56761 gcccattcac atattccgaa gtaggcatcg taacagattg gccattatag cttcctttaa
    56821 tcccaaatag attgtaatta ggggcagaag ccaaagtact gttcccccag ccactttcaa
    56881 cgatggcttg agcaatcatg actgatgcat ataagtcatt ggcacttgcc aaactttgtg
    56941 cagttgaggc aatactatca atgaacgctt gttgagaatt agcagatgag cgagcataaa
    57001 aagcatagcc agtatagttt tcgaattcag acaaatctgc ttgatgggta tgtgtcaatg
    57061 tttgcccatc tgtcaattgg ttcgtgattt cttctgcatt ctctggattg ttctgagctt
    57121 ctgctgatgt actgtcttcg gtttgtgttt gttctgttgt ttcagttgtt ggttcttcgg
    57181 ttgaaattgt attttctgag acagtagctg actgcaaagt actctctgta tcgttttcaa
    57241 ttcctgtttc tgaagaactc gatgtctgag ttgcgacagt ttctgtcgat tcttcagcta
    57301 gcactgttgt gttggcaact gctagatttc ctaaaagaat aacgctaaat aataaattct
    57361 ttgctttcat cttttttctc ctttgaatta tgaaactttt cctaaataac agtgtaacta
    57421 aaaaagtaaa agcaagtcaa tgtccatatt taaataacat ttgtttcttt ttgataaatt
    57481 tgctttttct acataaaaag acgtaaatga agaatattct tctccattca cgccttaaat
    57541 tgatggtcgt ttgaataact atttaacaag taattctctt tggatattat ccgttatgga
    57601 ttatacttcc tgtgtcgtcg gacggataat catgttactg acagacatcc ggtctggcgt
    57661 ttctatcgca aatgctacag cagaagctat atcttcggag gtcaatcccc attctttttg
    57721 cgcttcatga agttcaagag cagccttttt gtcgctgatg gtttgatata attcagtttg
    57781 aacagctcct ggagagatga tcgtagattt gatgttattt tgccgttgct cttgacgtaa
    57841 tccttccatg attgcacgga cagcaaattt tgttccgcaa taaacggcgg aatcaggata
    57901 aacaacatgt cccgccacag aatcagttga aatgatatgc ccagattttt gtttttccat
    57961 gattggaagt acggcggaaa ttccatttaa cacacccatg atgttgatat caagcatatt
    58021 ttgccattca tcacggtgac cttcaactaa tggagcggtt ggcatgattc ctgcattatt
    58081 gtacaataca tcgattcgac cgaacttttc catcgctaaa tcgataactg cttggacttg
    58141 tgcaaaatct cttacatctg ctgtttgaat atatagctca gcttctggta aagattcttt
    58201 tattgctttt aggcgatctt ctcggcgtgc agctataact agtttagccc ctttttctgc
    58261 taatagcttc gttgttgctt caccgattcc gcttgaagcg cccatgatga caatcacttt
    58321 ttctgataaa tcattcattc tattctcctc ctgttttgaa aaatctcttg cttatactca
    58381 ctatctctta tttcatctta ctgttcaaat aatccgttcg acaggtttga cgattttggc
    58441 agggattcct gcaacgatgg tattgtctgg gacatctttc gtaacagttg cattcgctgc
    58501 aacaatggca ttctcgccaa tcgttacgcc tggcagaata gttgctcctg ctccgatcca
    58561 agcatttttc ttgatgtgga ttggctttac tgacaagcct cttcgttctt ttggatcggt
    58621 tagatggttc accgtgatga ggttgacacg aggaccaatc aatactgaat cttctaaaca
    58681 gatgccacct aagtcaacga acatcacttg acgattgata aagacatttt tccctatact
    58741 aatatgtttg ccgaaatctg tataaaaagg cagcagaaca gtcaccgtat catctacttt
    58801 gtcactaatg atattttcta gccgtttgcg gatatctgct ggttcatggt acccagtatt
    58861 cagcgctgct aaaggctctt cgttttcagc ttggatctga tggatcactt caaagagcgg
    58921 atccgttact cgaatctctt tattgacgat ttgtttttcg atgaggcttg tcattttgct
    58981 ccctcctttt atccttactt tataacggac ggcttatcat gtctaatact tataaaacat
    59041 acttaactat tccttttaac tatacttagt ggtatgcttt tttaaaaaca ggagggaaaa
    59101 aatggatatt cgtatactga cgtatttttt agcagtagct agagaaaaaa atatcagccg
    59161 agcagcaaaa tctctgcata tcacgcaacc tactctctca aaacaactga aagatttaga
    59221 agaagaactt ggtgtgacac ttttcactcg gggctcccga gagatacagt tgacggattc
    59281 tggtcgatat ctttattcaa aaggaaaaga aatcttgtcg ttggtagaaa aaacggcgaa
    59341 caatttaaca aatgacgcaa tcatctctgg agaaatctac attggcggag gcgaaactaa
    59401 agcaatctct ttcatttctg atagccttca ttcgttgatc aaggcgcatc ctgatatcca
    59461 gtttcattta tatagcggga atgcagatga tatcatagac aaactggata gcggattact
    59521 tgattttgga ctcgtaatcg aaccgacaaa caaacaaaaa taccagtcat ttcgactgcc
    59581 agcccaagat cgttggggac ttttgataca tcgaaatcat cctttagcaa agcaaaccgt
    59641 catccatccg caggaactga aaactgttcc tttgatcatt tcccggcaaa cgtcagtgga
    59701 cagtcaacta gctgaatggc taggtttcag catagatcag ttatcgatcg tcggaacata
    59761 taacttattg tacaatgctt ctttgatggt cgataacggg acttttggtg cattagctct
    59821 agatgggatc atcaatactt atgggacgga aaaaatattc atcccactcg atccgccatt
    59881 acaagcttca ctgaatctca tttggaaaaa agaccagcct ctttctcctg ctgctcgtaa
    59941 atttttagat gtcctaacgg agaaatacag ttaagtagag tttttttcct tcgctttcag
    60001 ctttttaaca acgaataaga taagcaaaag atccaacgat taaataacat aagtttctta
    60061 atttatttct tccttttatt tctgataatt ccagtaaatt actataatta taaaaaaaga
    60121 attgacaaaa cataactgtc tatataaaat tgattacgtt aacaataatt cgtttcatgt
    60181 gactagatca tactaggcat gaaaaatctc tgaggaacat ggtttcccta ggaggttttt
    60241 cgtgtctatt tttgtttggc cgcaatcaac tggtctagca aacgtgaaaa ccaaatcttc
    60301 aaaaagggtg cgcgcatgaa aatcaaaaag atctttaatc aaaatgcagt attggtagat
    60361 gacggtggac aggaaaaagt agctattgga aaagggattg gatttgacag aaaaagaaat
    60421 gatttgattt ttgatcgtga tgtcgaacgg atatttgtca tggaaccaga aggtcaaatc
    60481 aagttgcaaa atttacttaa tcagattgat gaacgctttt tatttgcagc agaaacgatc
    60541 attgatcatg cggaaactgt tttgatggaa aagttgaatg aacatgtatt gatcgctttg
    60601 accgaccaca ttgctttttc tgcggagaac attaataacg gtatcgtcat ccgcaacaaa
    60661 cttttaagag aaatcgaagt gctttacagt gatgaattca gtattgctca atgggcagta
    60721 gagtatctga caaaagagct tgggattcca tatacttatg acgaagcagg atatatcgca
    60781 atccatattc acagcggacg aagcggtcgg aacaataacc atcgcagtat ccgtgaagtg
    60841 acgattgtat ctgacatcat ccatttagtg gaatcagaat tggaaacaga tatccattca
    60901 gaaccatttt cactgaatta ttcaagactg gtgaatcatt tacgactgct tctacaacga
    60961 acccatgcgc aacaatatgc tgttttagac acggaaatcg tagaaatggt caaacgaaaa
    61021 tacccagaaa gctataagat ttctaaaaaa atccgtgtgt tgctgaccaa agaataccag
    61081 ctcgcgatca cgaaagaaga actgggttac ttggccatcc atatcgagcg tttaagaagc
    61141 gcaatcgtac aaacaaatgt taacaatcat gaggaggaaa aaaattgaaa gcatatctac
    61201 aacgaatggg acgttccctt caacttccag ttgcggtctt acctgctgct gctttactag
    61261 tagggatcgg aaactggtgg gcatcttata gtaacgacat tgtcgctcat tttttacaag
    61321 ctggcggtaa cgcagtttta ggtcaattac ctttgctgtt tgccgttggg ttagcactag
    61381 ggatgtcagt ggacaaatca ggtgctgctg ccttggcagg tttggtcgca tttgaagttc
    61441 cagtaaatgt tttaaaaact gattcagtag ctactttatt gaatatcaaa ccagaatcag
    61501 ttgatccatc ttttgctcag atcagtaacg tatttattgg tattattgcc ggattgattg
    61561 cggcagctct ttataatcgt ttccacgaag caaaattacc gatggcttta tctttcttta
    61621 gcggcaagcg tctagtgcca atcttggctg cctttgtcat gttgctgatc agtgcagtgc
    61681 tactgctcgt atggcctccg gtttactctg ctttagtttc ttttgctaaa ttcattttga
    61741 gtttaggctg gggtggtgca ggtctttatg gcttcttcaa ccgtttactg atccctacag
    61801 gattgcatca agcaatgaac tccgtgttct ggtttgacgt agcaggaatc aatgatattg
    61861 gtaaattttt ggcttctgaa ggaacaaaag gcgtaacagg aatgtatcaa gctggatttt
    61921 tcccaatcat gatgttcggg cttccagcag gagcatatgc tatttacaga aatgcacgtc
    61981 cagaaaacaa agcaaaaaca gcttccttga tgctagcagc agcttttgct tcattcttta
    62041 ctggtgtgac agaaccactt gaattttcat ttatgttcgt tgcttggcca ttatatgtct
    62101 tgcatgcaat tttcactggg atttctttag cagtgagtgc attcttccat tggacagcag
    62161 gatttgcctt tagtgccgga tttgtcgatt acttcctatc tttgaaaaat ccgattgcga
    62221 atcagccatt gatgttgatc gtccaaggat tagtaacagc agtgatttac tactttggct
    62281 ttaactttgc tatcaaaaaa ttccatctga tgactcctgg ccgcgaggaa gcagatcttt
    62341 cagatgacga tactgcaacg actaattcag acaataaata tgcggcacaa gcaagcaaaa
    62401 tctacgctgc attaggcgga gccgacaatg taacttcgat cgataactgt actacacgcc
    62461 ttcgtttgca agtcaaagat acaggcctga tcgatcaaaa taaaatcaaa gccactggcg
    62521 tacctggtat gaagatcatc gacggaaaaa atgcgcaagt catcgtaggt acagaagtac
    62581 aattcgttgc agatgagatg gcaaaactac atggtggtgc tgcagcacga cctgcacaaa
    62641 caaacaccgt ggttaaaaca gaaacatttg ccggtgaaac gatcgaacaa gatatttacg
    62701 ctatcgcaaa tgggaaactg attccaatca ctgaagtttc agatgatgta ttcgcggaaa
    62761 aaatgatggg tgacggctat gctgtattac cagaaaatgg agaaatcttc tctccaatcg
    62821 ctggtacgat cacaaacatt ttcccaacga aacatgctgt cggaatccaa acagatgctg
    62881 gtatcgaagt cttgcttcat atgggcatca acactgttga cttaaaaggc gaaccattca
    62941 ctttatatgt agaagaaggt caaaaagtcg caagaggtca attgattgct ttagttgatc
    63001 ttgccgctat tcaatctgca ggcaaaaaca cggatatggt cgttgtattc acaaatggcg
    63061 acaaagtcca aagcttagaa atcgaacccg cacgtgatgt aaaagcaaat gataaaatcg
    63121 gctctgtttc caacaaagcc tgagccatcg tcttccccag acttttttat cgataaacaa
    63181 acaggagctg agacaaaaat cttgtctcag ctcctttttc cgaataaaaa cggcgttctc
    63241 ctgccaactc cgcggtaata agtccagcta aacgaaacat atgaagagct attttttatt
    63301 tgactcttct tattacttga tgcagaacag ctttttcaca aaatttttct tcatactcaa
    63361 aactaatcgg ctttttcaca acctctttat acattattcc tgatccaata tttcttttaa
    63421 tgctgcttca agagaagtta atggttttcc caatactttt tctaaatcat cagaggaaat
    63481 agctaactga tcctttttaa tatcatgttg gatcgctaaa aacatgtcag ctgcttcttg
    63541 agggaatccg gcttctttta gattttcgac aaacgctgca tcacttgctt caataatttc
    63601 taactcttta cctgtcgcac gctctaatgc cttggctaat tcttcatagt cagttagatt
    63661 gccggataat tctaagattt ctggagagtc actttctact agtacttttg ctgcagcttc
    63721 tgcatattca cgtttcaatg cccaacctgt ttttccattt cctgcagcat aaacgaattg
    63781 accagttttt aaagcctgcc caatgatagg taattcattc tctacatacc aattattccg
    63841 taaaaatgta tgggaaatac ctgaatcctt aatcattttt tcagtgaatt ggtgatctgg
    63901 cgctaataca ctgatcgagt gatcggcatc tgcaaaactt gtataagcga tataagatac
    63961 cccagctgct tgtgctgcct tcactacatt cttatgttcg gcttgacgat ttcctggaac
    64021 gccagagaca aaaagcaatc gatctattcc tttcaacgct tctttcattg agtctaaatc
    64081 atcataatca ccaatacgta tattaaatcc agcttctttc agtgcttctc ctttttcttc
    64141 actacgtact agtacataga tttcattttg tggaaccatc ttttttaaga attccagcgc
    64201 ataaccacca aaacctccag ttgctcctgt tactaaataa tgcatatttg ccactcccta
    64261 ttctttttat ttgttaagat ttacagctgt acatttataa attacacctt tatcataaag
    64321 agtgtggtaa gataagtcaa gcaaaaaaaa tgaaaggatg acaactgatg aagtattctc
    64381 tgcaatttag tgatgcaatc catgtacttg cctatattga aatgtttcaa gatacccgat
    64441 cactttctag tgagatgatt gccagtagta tcgaaacaaa tgcagcaaat gtacgtaaaa
    64501 tcatgagtca gctcaaaaaa tccggtttga tctttacaaa aaccggaaaa ccgcagcctt
    64561 cccttgccaa aagtccagat cagatttctt tactagatgt ctatcgcagc atcgaaggta
    64621 atacacagct tatccaagtc gatccaaaaa caaatccaaa ttgcgtcgtt ggtgcgaata
    64681 tccaagaagc tttagaagaa agctacgctc ggctgcagaa aaaggcagaa gaagaaatgg
    64741 ctaagatcac acttgatagt ctgatacata aaatagccgt tttggaaaaa gaaaaaagac
    64801 cagaaaacac aggcctcgta gagagatttt tataattctc tctactgtgc ctgcattttt
    64861 ctgatcggaa tcgtctacat aaattctttt attctccggg ggacaaaagc cttgtcatgc
    64921 ccctttttcc tctctaatgc tcaggtcaaa gcgctgaagt catgacgtct tgataaacgg
    64981 tgttcaagta gctgaccctc ggcattagtt ctgaaaaatg caaaatatgg aaagctattt
    65041 cccatttttc ctctctaatg ctcaggtcaa agcgctactt tcacgacctc tatattatca
    65101 tggtttattg actaccggta tccagatttc tcccatatat ttcccgttgg taactcccat
    65161 ttcgactaca gcatttggac ccccaacata ggcgtaatct tctacttctc ttaaaacatg
    65221 cccaaaggcc gtattcgtca aatcatttct taattcttca ggcgattctg ctaaagcgct
    65281 tacaacaaga tacgttcctt ttgggaatgg gatgattcgc gtagtgtttg gaacatcctg
    65341 actcgtttga acaccgacat agtacatcat tttcccgtta tagggttcgt tgactgtaaa
    65401 gaaataatga ttttcagcag cttgtttcaa tgcatctatt gttccattac cgataacttt
    65461 ttgctcaaat gcttgtttct ctgcagccaa accgacaaaa tccgtattgc tactcttcaa
    65521 ttccacacca atacccgtca ccgtaaaatc tggtttttct tcgatcctat actttgccat
    65581 ttctcccacc ctttctatta tattaagttc atcttacata aaattaaagg gaaatgacac
    65641 gattacgctc ttttttctag ggatattttt attttttaac tatttttttg ctttcactgc
    65701 aatccaaatc tcactgtaaa cattactgaa atcctctccc cattttgtaa aagagatctc
    65761 gggagcttcg actaacccat aatctgaaga aggaagccat tctgcataga ttttccccca
    65821 agtatcttgt aaagtttggg gaaatggccc tttattagga aaaatggccc aagtgtgttc
    65881 ttctatagca agcatttcta aatcagcgta cggattttct tttgtcgtag caaagccaat
    65941 catgtgcatc aaactgccct tttcttccaa gcggtctcca tcaaatccat aagaaacgtt
    66001 gattacttga tttggatata aatctcctaa cttatgcatc tcttcccttt gagtctccgt
    66061 gatcgtctgc gctaactttt gtatttcttg attgactcct tcaaatttga caggaacttt
    66121 tttcgccaca cctgccacat aaaactgttc tttcttttcg atacgaaatt ccatcgattt
    66181 tcctcctctg acatcaataa aaaatgtcaa gcgtggaaaa gatttttgta tgccgtatct
    66241 actgatttct gaaggcagat atcctgacca atcacgaaaa gctcttgaaa aaccttcgac
    66301 tgtttcatat ccatatctaa acgccgtttc tgtcactctt gatccatcta gaagatcgcg
    66361 attagccaaa gccaatctac ggtttttgat atagtccatt agcgacatcc ctgcaataaa
    66421 cgtaaagatg cgttttaatt gatattcaga gattcctgta ttttcggcta ttattttata
    66481 ggaaagtcct tctgtcaaat gttcttcgat gtaatcgatc aattggttca gatctttaag
    66541 catatttatc ttcctccttg attgcctttt tatcatatta tcaaagaaag tcaattactt
    66601 gatgaatcag gaaccaattt agtcagatgt agaaaatgtt gtatggatct aaagaaaatt
    66661 ctcaagcagc gtttcgtaac ggataattgg tattatctgc ctatctatgt tttaccttta
    66721 gaaaaataaa ttttggggaa gacagattct aagtaaacaa agcggtaaaa atatctttta
    66781 ttcccttagt taatccgctt actacctact gtacaaagca aattgttgtt caaactgctg
    66841 atcagaacgc tgcttggatt tttctttgcg atacatcata cctcttttat caattggaac
    66901 aattatttat ttctaacaaa atcacttcat ttttgtcaga taaataaaaa acaaaaaaag
    66961 ttccatcttt aaaacacgct attcttttac tttttctcac attctctaag aaaagaaaag
    67021 gagaatgaat ttttgacaat tcgagcaaaa gtgattatca aatatgtaaa atcagctact
    67081 tttttcttaa acgtttcttt atactaaata taaataagtg tttcatgaaa ggagaactag
    67141 atgaataatt ggcttgagta ttttcctgaa aacgttttgg aacgcggtta cagctatcat
    67201 ctgcacggtt ttgtccgtca tttgaactat acaagcaagt acttgtccgc aacagtttct
    67261 ggcacggaag attataaggt agtgatcact tgggatgaaa agaccaacat gacttgtgat
    67321 tgtctatatg ccatagaagg aaaaaaatgc aaacacatgg ctgctgtctt attcgcttac
    67381 gaagaacgtc caataaaaaa atccaactac tcactttccg aactctcgtc ccttgtttcc
    67441 tccgctagtt cctctcttgt acgggagctt ttaacagaga ttcttataga acatccgcac
    67501 tttatcgaac gcttcaaagt taagatgcca tttcatgcaa taaattattc agataaatta
    67561 acaactatca tccataaata cgatcatatt atcaagaaaa acaaaaacag gaaaacagcg
    67621 aagttcatca tggagatgcg aaagttcatc caagaagcag ttgaatcgct tattcagcaa
    67681 aatgcctatc ttcccgcatt cgaattgatc aatgaagtga ttgctactct cgaaacattc
    67741 tattgggagc ctgaggatga acgaaccctt cttctgatag aggactgtta ttatttatgg
    67801 aaagaattat tagcagaagc acctcatgca gaaaaaaggc aaatgttctc ttggttcgtc
    67861 tgccaagtcg atcatactga cgcctcttat tcaaaacgct actctataaa aatcctgaaa
    67921 gaagactttc gggaaaaaga attttctaac caaaagaaaa aaatagataa aaaactaaag
    67981 aaacggtaaa gaaagacgat ttcaatgaaa aatagcaagt aaacgataac aaccatccta
    68041 aatccacaag aaatacagca gaccttgctt taaaaacacg aatcatgcaa aggatgaagt
    68101 aaagaagact taacgcgtac ccaatattag tcatttgact attaggtacg cgttttgttt
    68161 tataagtcat gacaaatttc cttcgttgct ggatagattt tccggcattg ttgatagatt
    68221 ttatgatact tttctacatt ttgtaagaga ggttccgctg catctgtata ttggataaaa
    68281 ttctgacaac aactttcagg tgtgtcgaac caccctaacc ctagtgcagc aatcattgtt
    68341 gcgcctaatg caggtccttc ctccacactt aaacaataga ccgttgcatc gaaaatatca
    68401 gcttggattt gtagccatgc aagatttttc gcgcctcctc ccaccgaaac aatacgtgta
    68461 aatctgcgac ccgctagttc tttacgtttc aatcccacca ttgacaaatg ctcattaaaa
    68521 atttagaata ttcacaagtt taacgacaat tatgaaaatt tatgtcgtgc taaaggagtt
    68581 ttgttattgg atgaaaagtt agatataaaa actgcgatca aagacacgtt acctaccgtt
    68641 ttcggttata tcggtattgg acttgcattt ggtatcgttg ggaaagctgc cggatttcat
    68701 ccactagtcg tcacgttgat gtccctgctg gtctatgctg gttctgccca atttatcaca
    68761 gtcagcatgc ttgctagtca cagcccattg ctttccatcg ttttctcgac ctttctagtc
    68821 aattcccgaa tgattctgat gagtatgacg attgctcctt atttcaagaa aaatcggctg
    68881 cttcaaaatc tgctgattgg cacattgctg acagatgaaa gtttcgcact gggaatgaat
    68941 aaattgaact atactggaca aaagttaaac tttcgttgga tgaatacagc taattggatt
    69001 tcttatttga cgtgggtggg ttcttctctt gttggcgcac ttttagggaa ttttattact
    69061 gatccgaaaa agtttggttt ggatttcgcc atcgtcgcta tgtttatcgg gttactttat
    69121 cttcaagtca tttctgatag aaacacctct aaacgattac agttgatcct gattggtctc
    69181 actttgattc tggtatatgt cggactgatt tttatcccaa gcaacttagt gattgtagtt
    69241 gtaacactga ttgggtgcgg attgggggtg tggatcaaac atgccttctt ttgagtttac
    69301 attgctgact attttaggtt gtactttagc aacttggctt tctagggtcc taccatttgt
    69361 cttactgaaa aaattcgatt taccgcaacc gttgcttgag tatttaagtt ttgtcccgat
    69421 cgtcattatg tctgctttat ggttcagcag tctatttacc caaaatatcg gacatctgcc
    69481 gcagatcaat ctcgaaaatg ctttggcttc agttcctaca cttttagctg ctattctttc
    69541 aaaaagctta ttggtgatcg tcttggcagg aattctttcc ctttcgttga ttcgcttatt
    69601 ataaataaaa aaacaagagc ttatggcaaa ttactgtcat gcgctcttgt ttttatcaaa
    69661 gtcaaataag aaaaaaatcg gcgtattctt gcttatccgt tgaacggacc actaacaacg
    69721 acttttccta acatatgtcc tgtttctacg tctttggttg cttgtttcaa gttcttcgca
    69781 ttcatcccat ctgaatagac cttgctcaat gtactgcgga taatcccttc atttgctaaa
    69841 gcagcaatgt gcgctaaagc ttctccttgc gtttcgattt catagttata gtctgtttta
    69901 gcgaacatat actcccagtc gaagctgata gattggtttt tccaaacacc tatatctaac
    69961 ggctctttga tccctacgat cgttcccaca tgtccaaatg gtcggacaag gtgcttgatt
    70021 tgatcaaagt agcgggtaat atcgaataat acagcgacat actctacttg atcgattcct
    70081 aattcacgaa aacttttact taagtcgtga tgataatcga ttggatagtc tacacctgtt
    70141 tttttcagcc attcaaaatt cttcggactt gcagtcgcgt agacttctag gccagcccaa
    70201 tgcgctagtt gattcaaaat cgaaccgacg cccccggatc cgttgatcac taagattttt
    70261 ttcccgcgat tggcattttc ttctggagtt agaccaaatt tttcaaataa caattcataa
    70321 gcggtcaatg aggtcaacgg caaggctgca gcttcttcat ctgaaagatt ttttggtgca
    70381 agagcgacaa tacgttcgtc aactagttgg tattcttgat tgcttcctgc tcttgttgtt
    70441 gtacctgcgt aaaaaacgcg atcgccgaca gaaaattttt gcacttcgtc acctacagca
    70501 acaactttcc cgactccatc aaaacctaag atacgtaaag catttcttat cccgttatct
    70561 tggcgaagtt tcgtatcgac aggattaaca gatactgcgt tgacttttac cagaagatcc
    70621 cgtgaactgg gtacaggtat ctgttctttt tcatcaataa agctttttgg atcatcgatc
    70681 ggcaaccctt cataaaaacc gactaccttc attaattcct tttctgacat acgaattcct
    70741 ccttagcttc atcaagttgg agcatccata cagtgttcat ttcaatatat atagtatgct
    70801 atttgttcca ccttttttat tcatcataat tattctctta cttaaagtaa actatttgca
    70861 cttttttggt taaaaaagag gttgcgacaa gacttttgtc acaacctctt ataaacggtg
    70921 ttctcctacc aactcttcgg tcacaagtca aaatattcat caaacatgag aagctgtttt
    70981 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct gatataaacg
    71041 gtgttcaatc aactgaccct cggtattagc tcaaaaaacg acaaaatatg aagagctatt
    71101 tttgtttggc tgttcttatt acttgataca ggacagcttt ttcacgacct ctaattcacg
    71161 gtgttcaagc aagaaatcat tgttcactga aaagcgttgg atgttctttt tcaaaggtct
    71221 ctaagatctc tcgtacttca tcagtagatt tcgtattcat caactgaact cgcaagtcat
    71281 ttgctcctgg aaatcctttg atatagattt taaagaaacg atgtagtcct gtgatggaac
    71341 gcgggatgat ttctgaataa tgatcttgca gatctagttg cagacgaagt agtccgatca
    71401 gttctttttc tgtatgttcc ctcgggtctt tttcaaatgc ataaggattc ttgaaaatcc
    71461 ctcggccaat catgatgcca tcaacgccat atttctcggc caattcttct cctgtctggc
    71521 gatcggggat atctccattg atcgtgatta gtgtttgtgg agcgatttgg tcacgtaaag
    71581 caatgatttt aggaatgact tcccaatgtg catctacttt actcatctct ttacgcgtac
    71641 gcaaatggac agaaagattg gctatatctt gatgaagtag gtgactgatc cattcttcca
    71701 tctcagacat ttccgtatag ccgatacgcg tcttgacgct gacaggcaaa ccgccagctt
    71761 tagctgcttc aatcaattgt gccgctactt ctggacgaag gatcaagcca ctgccttttc
    71821 cacgttctgc tacattaggg actgggcagc ccatgttgat atcgattcct tggaagccca
    71881 tttctgccat agcaatgctc atttctctaa aaaattctgg cttgtctccc caaatatggg
    71941 caaccatcgg ctgttcatct tctgtaaatg ttaatcttcc tcgtacactg tcttttccat
    72001 ctggatgaca gaaactgtct gagtttgtaa actcagtaaa gaagacatcc ggtgctgctg
    72061 catgctttac gacatggcga aatacaacat ctgttacatc ttccattggt gctaaaacaa
    72121 aaaaaggctt tggcagctct ttccaaaaat tagtagtcat ttatttgtta ctctccattc
    72181 tatcaagcag tgaagtcgca atctttctca aaaacattgt cattatacta ggaaacaaaa
    72241 aaagaagcaa agtgaacagc ttaaaagatg tttctgattg agcaaaaaaa agactgtgac
    72301 aaaaatctta tcacagtcca ttttccggaa taaatggtgt tcaaaagctt gcatttgcgg
    72361 taataagtcc aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac
    72421 ttgatgcaga acagcttttt cacaacctct ccactaaacg gtgttcaatc agctgaccct
    72481 cggtattcgc tcaaaaaaca acaaaatatg aggaactatt ttcccgtttg tttctctaat
    72541 actcaggtca aaacgctgat ttcacaacct cttttctaac ttctcaatcc tcttccttta
    72601 ttgatcaaat accaagtaac ggcgtacaaa acaacgatga ataacacaag tacgatcatc
    72661 gatgttgcta ctggcacatc gatcgttcca aggaatccgt aacggaaacc ggagatcata
    72721 taaacgatcg gattgacttt tgaaacagct tgccaaaagg gcggtaacat agagatggca
    72781 tagaaaacgc cacctagata agttaacggc tgcaagacaa atgtaggcac gatcgataca
    72841 tcatcataag actgagcaaa aatcccgttg agcagtcctg ctaatgaaaa caagattgcc
    72901 gtcatcaata atgtaatgat gaccatgaac catgaataca catgaagtgg tacaaagaac
    72961 aaggaaatga tcgttacgag agagccgacg agaatactgc gacccacacc accaatcaca
    73021 aagccccaaa taatcacatg cgtaggaaca ggtgctacca agatttcttc gatatttttc
    73081 tgaaattttt gagagaagaa ggacgaagaa acattggcat acgaactagt gatagcagac
    73141 atcataatca aaccaggaac gatgaattcc atatatgaaa aaccacccat gtcaccgatc
    73201 cgcccaccga tcatttttcc gaaaataaca aaatacaagg atgtcgtgat cactggcgga
    73261 accagtgttt ggacccatat acgcaaatag cggttcgttt ccttgatagc taaacttttt
    73321 aatgcagtaa aataaagatt aaacattttt ctcctccgta tgattatttt cttctgtgat
    73381 tttcaagaac agttcttcta aacgattcga cttattcctc atagaaagaa ctttgattcc
    73441 ttgtcttgta agctgatcga agattccgtt caccccttga tttcgttcaa cttcgacagc
    73501 gattgtctgc tcatcttcaa atgtacaagc atatccttca atcacaggtt tggtatcata
    73561 tggcgctaaa tcaaagataa atgtttcaaa ttgcagtttt gacagaagct ctttcatact
    73621 tgtattctcg atcagttcac ccgattggat gattccgatg ttgcgacaaa gcatttcagc
    73681 ttcttctaga taatgcgtcg tcaaaatgat cgttgtacca ttttcgttca attctcgtag
    73741 aaactcccac atctcgcgtc tcaactcgat gtcaactcct gctgttggtt catccaagat
    73801 cagcaaacgt ggttcatgca tcaatgccct agcaatcatc agtcttcgct tcattccccc
    73861 ggaaagcata cgtgcacgga catttctctt ttcccaaaga ttggactgct tcaaatattt
    73921 ttcgctgcgt ttcatcgctt ctttgcgaga gacaccgtag tagcctgctt gattcacgac
    73981 gatttgctgg accgtttcaa aagggttaaa attgaactct tgtggaacca atccaatctg
    74041 ttgctttgct cgaactagat ccttgtccag atcataaccg aagacattca cttttccaga
    74101 tgttttattg actaaagaag taataatccc gatcgttgta gacttcccag ctccatttgg
    74161 ccctaatagc gcatagaaat ctccttcttt caccgaaaga tcgattcccc gcaatgcttc
    74221 gaccccactt ccatagatct ttttcaaatt atttatttca agcgcatttg ccatttattt
    74281 cctatctcct tatagacatg attcttaatt gtatcatgga atacagcaga tgaaaaaaca
    74341 acttggcttc cacacaaaaa cacacctgct aacaaagagg agatgtgttt tctagttcat
    74401 gatcatcttc gatagaataa ctatctaaac aactctggaa cttttaaaat cataaaagta
    74461 ccgacagcaa ctactacgat gacatataga agatccttat aactgaggtt cttgtctttt
    74521 ttattcatgt taagctttcc tccgtccttt tatcgaaagc ttttttcttt tattttaacc
    74581 gaaataaata aaatggaaat agttattttc aatttctgtt ttcgctgttt gttttacgga
    74641 gaactttgcc atggaacgtg cgtctcgtcg ctgatgctaa gttttttcga taaaagctgc
    74701 tagaaggaag acagcgtggt ttattttgta tcgtcttttg ctaaatcttc caagatcatc
    74761 tggacagcag actcgttccc attgaattct gccacttttt tggcatcaat gtacttcttt
    74821 tgataatcgt ctttttggac ccacgcatca tgcttcgttt gataaagtta atcaaacgga
    74881 taccactgac gctgttcatc gaacaacaaa ctttcttttt gggcctgttc aactatggat
    74941 ttaggataat tcaaaacttt cagcaaggca atcgcatttc gggtccttga aggtccttct
    75001 tttaatttga aatcaaaact gattcctttt ccttctgtta cttgttcttc aaagtggaca
    75061 ttctcacaat aattctttaa aatctctgtc aattcgatat catgtgtggc gacaaatgct
    75121 aaagatggat actccgcaag ccaccgaacg actgaagcgg aagaagcaat ccgttcgatc
    75181 gtatttgttc cttttaaaat ctcatctacg aagcagtagc agcgttcttt ggaagccact
    75241 tggtccagca aacgtttgat cgatttgatt tctgaaacaa aataactgtc gccttcaaat
    75301 aaatcatctt cgattgccat ggaagtcaac acatgtccag gttgcagggt aaactgttct
    75361 gcaatcgctg tttggatcgt ctgtgctaga atacagctga tggctatact tttgacatac
    75421 gtagattttc ctgatgcatt ggaacccgtg actaatgtat tcttctgcca gttgactgga
    75481 ttaactaccg cttcttttaa caatggatga tagatatctt tcgctgtcac accgccctca
    75541 gtaaactctg gtcggcaagt gatcggcata taggtgcgat aattcaaaac ggctgctgca
    75601 acttccaatt ctcccagcaa ttcccacagc ttgatggctt cagttgaatg cttttctaat
    75661 ttagtgagaa cgaaatgata ggagataaat ggcagcatga acatgatgtt gacatattca
    75721 aaaatcaatt ctgcttcact cccgctcttc attcgaaatg aaaaacctaa tttagggatg
    75781 tctcttaatg gtttagtttg ctgtttcagc tctttttgaa gtggtgtcgt catttttgct
    75841 aactgattgg cggctgaaat cgttgaaacc aaataactca tgctattcag ctctgtttct
    75901 agtgtttgtt tcttcactag ataatagacc gtattgaata ctaagccacc gattgctaga
    75961 tagattccta gtacttgccc taaaagaagc aaacctacac ccaccagcgg aagcaagcca
    76021 agaaaaataa ataaacctac gcgtccaatc tttttacttt ttccatccgc tagatattgt
    76081 tttgcaaaat tatgatcttt ttttcctaaa cgagcgaatt gatattggat tctttctctc
    76141 agttgaggat tttcctgata aaaagcaatt agtttttcta actgataatc ttctccaaaa
    76201 tcaaagctac gtaaacgctg atacaacgct tctgaaccta cactagaata ggtactgttg
    76261 atcccttcaa atacttcgaa catatccaag tcataccaag taagatcgtc gatttcacta
    76321 tcccatgaac ggaatttttt ctccgtgagc cacgcatcct tcaaactttc ttctttatca
    76381 aatctcggtt gataaggaat ttttccccaa ttactacgaa ctgtttcctt tactttcaaa
    76441 cgtgaaaaaa tgtctaaaac aactaataaa ataacaactg caataataat taaaacgata
    76501 aattctcttt ggcccatatc cctctccgtt ccttgatttg ttagttttag tatagcactg
    76561 ttgcgctatt tgaaaagggg gtacaaaaat aactgtcttc gttacctaat tcgtatcaat
    76621 cacaccaaaa taaaaacacc ttccaaaaaa gaagatgtct ttaaaacgag atacaatttt
    76681 ttgtcattca cttcaggtaa attagagcca attaaaccct ccgcaacttg acggagatgg
    76741 agaagatgcc cccgtctaat ctagcggtca tctctcctcc cattccttcc accaattttt
    76801 ttgacaggta aagcccgatt ccgctagatt ccgtatttgt gcggctggta ttttcggtat
    76861 aaaaacgctg gaagactttt tcaacagagg tttgcgctcc tggtttgata ggattggcca
    76921 ctgttaatcg aatgaatgaa tcctcttttg aaaatgtcac ctttgcttgt ccatctgcat
    76981 aacgcaataa attctgtatg atattttgca gcactcgcag caacatgagc ggatctgcta
    77041 atacagttgt atttatcgag tgttcagcga acgctacctc gatatttttc tcagaaaatt
    77101 gttcataaaa agagaaaaat acttcttcca cctctttaga aaaagaaagc gtatggagat
    77161 ccatttcttt ttgcttcgtc tctattcgag caagatcata aaactgatcc gtcatactga
    77221 gtaatcttgc aacagattgt ttgatgatcg ttaaatactt ctcccgtttc tccgtatcct
    77281 ctacctgttc cagtaattgt acataaccgt taatagcagt caaaggagtt ctgagatcat
    77341 gagaaaggcc gacgatcgat agtctgatgt tttcttccat ttcttgaaat gcttgtcctt
    77401 tttgttcatt ttcatccacc catgtattca gtgcatctac taggtccatt agattctttt
    77461 ctcgaaattc gactgaaaga cgaccaccat attcttgtcg ttcagaaaga gtggcgattt
    77521 tcttagttaa tctcttgatt tccctttgca ggaagaaaat aaaacttccc ataaagataa
    77581 caacgagaag caacagccac ttaatcaaat ctaaacccca ctccccacac cgtatgaata
    77641 tacacagcat gtgcctttgc tagtttccct cgaagattgg aaatatgcat attcactgtt
    77701 ttctcactat caaaatatgg ttcttgccaa actgtttcat aaagatttgc tcgcgaaaat
    77761 acttttttcg gatgttctaa aaaaagcttc agcagatcga attctcttcc tgtcaaatgg
    77821 acagccacac cattcacatt tacatccctc gactctttgt ccagtaagat ttccttgtaa
    77881 tagatgcgtt gcgacagatc ttttacttgg acagaagtct gatgacgaag ttggatgccg
    77941 attcgtaaca acagttcttc gatatcaaat ggtttcgtca catagtcatc cgctcctgct
    78001 gccaacaagg aaactttatc tctttgatcg ctttttgctg aaagaacgat gatcggaaca
    78061 ttcgatgtct gtcggatttc tgctaacaat gcttcacctg aaagccctgg aagcatgaga
    78121 tctagaagga tcaaatcgac aggatatgtg cttaacaata attttccttc cgtacctgaa
    78181 taagcatcaa gaagtttgta tctctgttca agaacctcct ccagtaaaga atgaacagct
    78241 tcatcgtctt cgataatcaa aatagtttcc agcaggaaca cctacttaat gtcagatttt
    78301 tggaaaataa acattcctag agctgatgat accacgattg ttactagact gaccagaatc
    78361 attttcggtg cttgcgaata agttgcttgt tgcgcaaagt ccgtatacat gagccacgtt
    78421 tccgtaaacc actccgttag aaattccaag cctttgattt ttgcgaaaac accgggaacg
    78481 aaggccgttc ccatgatata aagaaaagaa agaagcaaac tgacactggt acttctagtg
    78541 atgaatgtca aagccactgg aacagataat gtcgctaata tcagcagaag caatacagga
    78601 aatactttta gaacataatt tccatagttt ttgattccct caacttcacc aaatccgaaa
    78661 gcaagtgtgt aagcaatcat ggctagcccc gtaatcaaaa ccacgccaag tgttgtgatc
    78721 aaaagcgtaa cgagaagttt cgacacataa taagtcattc gattccgtcc taaagataac
    78781 gtattgcgaa tcgtaccgtt ttgaaattct tccccaatat aaaagcacgc aaatgctgcg
    78841 aaagccagaa gaatccatgc agccattttt tccaccatta aaatccctgt tgcaccgttg
    78901 atgactgtat ccgttgcttg gtttaattca ttgccgtatt ttgtatgcat ataccattcg
    78961 ttccaagaat aaaaaagcgg aagtacgacc atcagtccta gtgagaccca cattttacgg
    79021 ctcttccata cttttatttt ttcagcagtt aatagattaa acatgtggat tgcctccagt
    79081 taattgtaag aaataatctt ccagtttttg cccaactagg tggataccat ccacaggaac
    79141 ttttgcctct accaatgctt gattgatggc tgctacttgg tcaattcctt caaaaagctg
    79201 gataacacgg ctagattgga caaaataatc cctataacca agagagtcga gaaccgttac
    79261 tgcttgttcc gtcgccgaag tgtcaatttt tataaattga cgagattctt gtgccaattc
    79321 ttctttcgat aattctttga tcaagtgtcc ttcatgcaaa ataccgtagt gagtggcgat
    79381 ctgtgacaat tcatctagca agtggctaga aagcaagacg gtgatgcccc gttcttttac
    79441 caaacgttgg atgatttctc gcatttctgc catccctgct ggatccatcc cattcgtcgg
    79501 ttcatccaaa atcaaaaatt ctggattcgt gatcagtgca tttgcaatag ctaaacgctg
    79561 gcgcatacct aaagagaaat tctttgcttt ctttttccct gtattttcca atcgcatcat
    79621 tttcagcagg tcttcgattt ctctatccga cacaccgccg ttcgctgctt ggatacgtag
    79681 gttatctctt gcagttaatt ctggataaag agcaggggtt tcaatcgatt gacccatacg
    79741 tcgtcttgct gcctgcaaat ccgtcgttcc aaataattcg atcgaacctt catcaatcga
    79801 gatcaaaccc ataatagttc tcataaaggt agacttaccc gctccattct ccccaatcaa
    79861 accatagatc attccacgtt tgatttcgat cgatacttta tctaatgctt tggcagcacc
    79921 gtattttttg gttataccag ttgctttcaa gaccgtttca tttccaaaat ccatacttat
    79981 ccttctttct tattgataag tgaagtatag ttgacgggta taaagaaagc agccagaaat
    80041 gtataaagaa agtgtaaatt ttccaattga atttcaagaa aaatcagact gtctcgcaga
    80101 cgtggttgag ttgtctgcca atcaaagagc gcaccaaaca gtgctaatcc aagtggactg
    80161 gccaccatgg taatcgttgt tatcgttgac atcagacgtc cttgtatatg cgttggaact
    80221 tctcgctgaa gccagaccat ataaaaagca aaagaaaaca ttaatcctta aaaacggact
    80281 tatgaaaaat aatacatgcc gtttgggatt tacaattagc taccaaataa gaggttatga
    80341 caaaagactt ttcacaatcc cttcttccga ataaacggtg ttctcctgcc aactcctcgg
    80401 tcacaagtca caatatttat caaacatgag aagcaatttt tgtaaattcc ttcttatttc
    80461 ttgaagctga ccacttctgt cacaacctca gaagcggata aataatattg tttgatgata
    80521 ctttatcttt ttcccgtttg tttatagatc gaatgatatt atagaccaag aatcctacct
    80581 attaacatag ctaaaaacac aaaaaaggca ctttccaaat tgtggaaagt gccagaaaag
    80641 ttaggattaa ggtaactgcg ctcagcattg cttcgcccag tacgattctc gtaactcgtg
    80701 aactcgaacg ataatcgcaa ttaacgtttt gagaactgtg aagctttacg ggctttctta
    80761 agacctggtt ttttcttcgt atcattgaat aagtttgaaa agccttgatt tataaaggtt
    80821 tttccgtaaa tatattttgt tatatttgat taaatttgaa atgtttgtgt tctaaaatgt
    80881 gttctaattt aaaggcagta tatttcgtat tttatattaa atttttaggc tcattcattc
    80941 tcaaatccat tctatttagt caataaatat tttaaaaaga ctttataaac aaagatgttc
    81001 aagcgagcat cttatttttt tgtgcctaat ttgaaaggag aacatttcat gtataaaaaa
    81061 caaaaaaaga aattatgtgt attaactatt ttgagtttgg ctgttcaatc aattgtagga
    81121 gcaattgctc ctacgattgc ttttgctgat gaaattaccc atccacaaac ggtaaccgta
    81181 gaactagatt tagcacatca atatgctgtt gaaggaactt ttagtgatgg tcgtccaatg
    81241 tcagaagtca ctgttccgca ttatgcagtg tacaacggcg ttaaacaaga tattttctgt
    81301 gttgaaccag gagtcccgat ttacaatgag ttcactcctg gatatgagaa gaacccgtta
    81361 ccagatatgt ccgataaagc caaattagtt tccgttttat ggaaaaaagt aggtacagat
    81421 gcagatactc atattgtggc acagaagatg atctggcaag aagttaatgg gtataccctt
    81481 cactcaataa aacgatcaga tggtagtgcc gtaaatatcg cagccattga ggcaaaaatc
    81541 aatcaagcca ttgccgatta tcagaagaaa ccaagcttcc ataatagtac ggctaaaaca
    81601 gtattagggc aatcgactac tgtgacggat acgaataatc tgaatttgtc agagttcgat
    81661 gaggttgtgg aaaataccgc aaacattgat tatcgtgtga atggcaatca gttgataatc
    81721 agcccaaatg ccaattcaaa agaaagtggt gtgttgacgc ttaagaaatc tgctggtacc
    81781 ggaacacctg tcgcctataa aaaggctggc caacagactt taatggccgg ggcgatcgat
    81841 aagccgaaca cttatacggt caaaattgat gtggaaatgg agggttcttt gaagattaaa
    81901 aaagttgata aagaatcggg tgatattata ccaggaacgg ttttccattt agactttgga
    81961 aaaactttac ccgcaaaaga cgtgaccacc gataaagaag gcattgcgac cttagatgag
    82021 attcctcatg gtacaaaggt aaccattact gaaaaatcgg tgccggcgcc ttatacgatt
    82081 gataccacac ccatgactgc cacaattaaa gcaggtgaaa ccatttcagt gacttcaaaa
    82141 aatacgcgag aaaaaggtca aattattctg gataaaaccg gaattgaaac agggagtgat
    82201 ctttggaacg ataattactc tttagcgggc aatacgtttg ccattcgcaa ggatagccca
    82261 actggtgaaa tcgtccaaga aatgaccacg gatgaaaaag gtcatgcaga aacaccaaaa
    82321 gagattgcca atgcgttgga attgggaacc tactatgtga ctgaaaccaa ggctagtcat
    82381 ggctttgtga atactttcaa accagtaaaa gtcgaattga attatgtcaa tcaaacagtg
    82441 gctcttgtta ccagtaacgt aaaagggcaa aaccaagaaa ttactggtga aaccactttg
    82501 acaaaagaag acaaagatac cggtcatgaa actcaaggga aagctgagtt taaaagagct
    82561 gaatacacct tgtttactgt taaggatgga aaagcggtca aatggaccga gaactttaaa
    82621 ccagaattgt tgaagggaac gaaagcttct gatgaaaccg taactttgac tttagatgaa
    82681 aagaatcaag ttgccgttaa acacttagcg attaatgagt attactggca agaaaccaaa
    82741 gcgcctgaag gatatacctt ggatgaaaca aagtatcctg tatccatcaa aaaggtggat
    82801 gatagcgaaa aaaatgctgt gattactcga gatgttgcgg caaaagaaca agtgattcgc
    82861 tttggctttg atttctttaa atttgctgga tcaactactg gcactgccga aactggcttc
    82921 aatgatctaa cctttaaagt gtcgccattg gaaggaacca atgaaatcac cggtgccgaa
    82981 gatgaagcga ccacagctta taacgagcaa ttagggtttg atggttatgg caagtttgaa
    83041 aatcttcctt atggggatta tttacttgaa gaagtagaag ccccagaagg ttttcaaaag
    83101 attacgccat tagaaatccg ttctactttt aaagaaaata aagaggatta taccaagagt
    83161 gattatatct ttactattac cgaaaaaggg caaaagcaac cgattaaaac ggtaacggtc
    83221 ccttatggga atctaaccaa tgagaatttc tcagtcagtt tgaaccgttt gatgctttat
    83281 gatttgcctg aggaagaaga tagcttaact tctcttgcga cttggaaaga aggagataaa
    83341 gaattgacta ctcttgattc taccgagcta gtcgataaat tgagctataa tctacatgaa
    83401 atcaaagaag actggtatat cgtagctcaa gccatcgatg tagatgctac aaaagctgcc
    83461 caagaaaaag ataaaaaagc caaaccggtg gcgattgcgg aaacaaccgc aactttagcg
    83521 aataaagaga aaacaggaac ttgggagatt ctgcataaat taactgtcga acaagtttta
    83581 gacaagacca ttgtcttatt caactatgtg tatgaaagca aagaagccta tgaagctggt
    83641 gatgagccgg tagcgaagga tgctagcttg aacaatcaag cgcaaactgt taattgtatg
    83701 gtggaacgtc atgtttccat ccaaaccaaa gcgcacttag aagaaggttc tcagaccttt
    83761 actcatggtg acgtggtaga tatgtttgat gatgtatcca ttacccatga tgtactagat
    83821 ggctcaaaag aagcttttga aacaattctg tacgctttat ttccagatgg tacaagtaaa
    83881 gaaatttgga aatctggcaa gattgattac gaagtgaatg acaaagaatt taccaaaacc
    83941 gtacttgcgg aaaaagtgga tacaagtaaa tatccagaag gtacaagttt taccttcaaa
    84001 gaaattaact atgacaaaga tgggaatatc aatggaaaac acaatgaaga tttgaaagaa
    84061 aaatctcaaa ctttaacgcc caaaaaagtt ccgacaacac caagtacacc agaacaatct
    84121 gaaatgccaa ctgttccaag tggctctcaa gaatctagtc ctacagtgaa ggcattccct
    84181 caaaccgggg agaaaacttc caatgtgcta ctgttcattg gctttagctt gatctttgcg
    84241 acggctggct attatttttg gaatcgtcgg aactaaggtg ataagatgaa acatgtaaat
    84301 agtagaaaac aatacgcgcg accgcctcct aaaaaagtgg tcgcgctatt tttgtattca
    84361 aaactaacaa tgaaaagagg aaaaaatatg gaattgaaat ttgttgtacc agatatggtc
    84421 gagacgttcg gaaaactaac atacggtggt gaaggtgaag ttctatcgga agggtatggt
    84481 cgaaatacta caattgttgg tcgaagttac catctgtttt caaataaaca acgggccgat
    84541 gatattgaag tggtggtagc tgcggaagcc ggcgaaaagg attttgacca agatcaacca
    84601 ctaaaagctg taaatcccca cttaattgcc aaaggctatg aaattgagaa tcgtgggttt
    84661 accgattacg tgttgtatgt tgatgattta gtgaaagcat aggaggaaaa gaagatgaga
    84721 ttaacagaag gtattgtcgt agattcagga ttaacctttg gcaagttgcg tttttccgca
    84781 ttacgtcgag aagtacgcaa gcaaaatgag gacggaacgg ttagtaacga ggtaaaagaa
    84841 cggacctata atttgaaatc ctccgcgcaa ggtcgaatga tccaagtcag tattccagca
    84901 aatgtaccct tacgcgagtt tgcttatgat gcagaggtag agctggtcaa tccaatcgtg
    84961 gatacggtcg ccaattatgt ttttcgggaa ggaaccaccg ttaattggtt tatcaaggca
    85021 gatgatttag tcttgaaacg acaactgaat caaggaaaac ctacgaacca aaatgaagga
    85081 aagaaatagg tgaagaatat ggaaattaat gttgaacata ttttagattg tctcgatcaa
    85141 tttggcaaag gtgagctaac ggaggaacaa ctcactaacg cattaaccta tgatgaaaaa
    85201 atgtttctga ttatgcatca agggttactg gaagtaggta acgatgccaa agaagacttt
    85261 gatgtactgg attggttagg ggaagaagag tctttcttta tggttgtcga ggtaaatgaa
    85321 agtttatgtc ggcaggcaga atctgttttg gaagaaatcg atgtggagat gccggatgcc
    85381 atcgaaagat ttttaaaaca attagtggaa acgaaacaac ttcccgtagt agtaaatgac
    85441 tagcataact ttttgaaatt gcccaatgaa atcctattgg gctttttcga ctggaggtgg
    85501 aaaccatcaa aatgtacaaa ggacatcgca tacgcgcagg agatcaacac ttgatttatc
    85561 actttgttct tggctggcta agtgccttat tcatcggttg gatgggcgtc ttttattttc
    85621 cagagcttag acaatttggt ctttctaagg tatcattttc cactattgaa atcgcttggt
    85681 ccatgaaaga tttagtatgt ctgcttggaa gtctcgctct ttcaggaagt atgatgttgc
    85741 tttatattca tttttttcct gatcattggc gaagtttgtg gaatcgacaa aagctagcca
    85801 gaatgattct agaaaaccat tggtacgaag tgaagcaaac tcaaagtgat ggctttttca
    85861 aagatttgaa taggagtcga accaaagaga ctatcagtta cttccccaaa gtttattatc
    85921 gaatgaaaga tggcttactg tctattcgcg ttcaaatttc actgggaaaa tatcaagatc
    85981 agctattgaa gttggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg
    86041 aactcaaaga ctcctatgtg gaatacacct tgttatacga tatgattgcc aatcgaattg
    86101 ggatagaaga agtagtggca gaaaatggga cgttgcgatt gatgaaaaat caagtatggg
    86161 cgtatgattc cttgcctcat atgttaatcg ctggtggcac aggtggtggg aagacctatt
    86221 tcttactcac cattattcaa gcactattga agtcagatgc agaattgttt atcctcgatc
    86281 ccaaaaatgc ggacctcgcc gatttaggta cggtaatgcc tcatgtctat tctcaaaaag
    86341 aagaaatttc taaatgtgtg gaagattttt acgaacgcat gatggctcgt agtaggtcaa
    86401 tgaaagaaat gcccaactac aaaacaggag agaattatgc gtatctcgga cttccgccca
    86461 actttttaat ctttgatgaa tacgtggctt atatgggtgc gaatcgtttt cctacaagca
    86521 tcgaatagat gtgaaatgga agcctattga gattatcaat agtgacaact aatgttttga
    86581 gagatggaat gaaagccttc aagttgagct gaaacatccc cgctaatact cctacatgct
    86641 ctaatcaatg aagtagattg gttttggtat gaagctaggt gaagtcgtct gaaatctgcc
    86701 taagtgcttg agcataaggc tgactatagg gcgggcggct gaaaaagata actatggtaa
    86761 gaatgtctga aaatgactga cgaatctgcg aatgtacggg tctaaaaggc tgatcttatt
    86821 gaaagatagg aacatacgga cgtatggtga gataccgaca agtaaggttc catgatatga
    86881 aaatcgggat agctaacgaa gaagctataa tttcacaggc tcatagtaga tacctaaggt
    86941 tatacatgat agctaaaaac attggaacga tgtaagcaga aaactatcaa ttaaatgact
    87001 acttctagat aggtgcatat aagtaataac gaaattgctt tgtttctgtg aaagtaaggg
    87061 cacagtaccg atgaagcgtc taacaaatgt ggagggatag ccctaagtct atttaataac
    87121 aatgaatgtt gaaacagatg aagttcaagg atctggtaag aacgtgggaa aatccgttat
    87181 ggaggtgcca cgcttgaaca caaaaatacg ttactgggaa tattatgatc tacaagaaac
    87241 attcgatcag ctatttactc aaagtagaaa cgggaaaaag ttttatcaat tatatgagtt
    87301 aatcatttct gaaaataata ttcttttagc ataccgtact atcaaggcaa acaaggggtc
    87361 ttcgacacca ggaacggatt ccttcacaat tgataactat aaggaaatga accaagcaga
    87421 gtttatccat cttattctca gtcatttaga aaattacaaa ccaaaatcca taaagcgagt
    87481 aatgatacct aaacccaatg gtgagaaacg cccattagga attccatgca tgattgatcg
    87541 aatgatccaa cagatgttca agcagatact ggagccaata tgtgaagcta agttttatga
    87601 acatagctac ggtttcagac cattgagaag tgcaaagcat gctctaggtc gcattatgta
    87661 tctcataaac atcagtaaaa tgcactatgc agtagacatt gatatcaaag gtttctttga
    87721 taatgtaaac caccgtttac tgataaaaca attatggaat ataggaattt gtgacaaaca
    87781 agtcttagct attctttcaa aatctctgaa atcacccatt caaggagaag gtatcccgag
    87841 caaaggaacg atccaaggag gtatcatttc tccactatta tctaatgttg ttctaaacga
    87901 cttagaccat tgggtctcaa agcagtggca tacctttgaa acaaagtatc cctatactaa
    87961 aggttacaat aaatttcgtg ctctaagaga tacaaatcta aagcaaggct atattgtcag
    88021 gtacgcagat gactttaaaa tcatgactaa tgattatccg acagcattaa aatggttcca
    88081 cgctgtcaag ctttacctaa aagacagact taaattagat atttccaatg agaagtctaa
    88141 aatcgtcaat ttgcgtaaat gtaagtcgga atttttagga ttcgctattt gcgtgaaaca
    88201 aaaagggaaa aaatgggttt gtaattcacg tatttctaac aaaaaaaagg atcagattaa
    88261 agaagagatt aaacaaagaa tcaaagatat tcaaaagagt cctacagcac aaaatgctct
    88321 cctgtttaat gctctcgtgc tcggtgttca taattatttc aggtatgcca ctcatattaa
    88381 tttagatttg aataggatga actacgttct ttcattcact acttacaacc gattatcaaa
    88441 ttgtagtaca agatgttacc ccaacaaagc ttccccaact tacaaaaagt tttactcaac
    88501 aacgatgaaa acgtataaag tagctggagt ctatttgtac cctctttgcg acgtatctac
    88561 aaaaacaatt tacggattta atacggagga tacaccgttt actccgtttg gtcgtcaaag
    88621 attggagcac aaaagcctac agtctcttgt gttccaagaa ttaagaaaat tgatggaatc
    88681 gaaaataccg aatagaacgg ttgagtattt agatagtaga atatctcgtt actcaatgaa
    88741 atcaggcaag tgtgagatta caaaacaatt tttgccagca aaagccgttc actgtcatca
    88801 ctatttgcca aaaagtcttg gtggagacga caaattcgat aacttgagaa ttattcataa
    88861 ggatatccat cttctgatcc atacaacaaa taaaatgata atcgatcact acgtgcatga
    88921 actgaaactg gtgccagagc aaatagcaaa aatcaacctt tatcgaaaga tgtgtaatct
    88981 gcaaaacatt caatagaaaa taaatagatg gaacgccgta tgaggtgaaa gtctcacgta
    89041 cggtgtggag cgggggaaaa gatggagatt atatcaaatt cttacctatc gctatagatg
    89101 ttaacgacaa aagaaagtgc ggtgattttg aataagctta aacaaatcgt gatgttaggc
    89161 cgtcaatctg gtttctttct gattctagct tgtcaaaggc cggatgcgaa atatctagga
    89221 gatgggattc gcgatcagtt taacttccgt gtggctttgg gtcgtatgag tgagcttggg
    89281 tattccatga tgtttggaga agtcgataaa aattttttta tgaaacgcat caaaggacga
    89341 ggttatgtag atacaggggg cagtgtgatt agtgagtttt atacgccact tgtaccaaaa
    89401 gggtatgatt tcttaaggga aattagcaac attgttggat taaccgttca tacggaaaga
    89461 gaaaataata gtgtgtagcc taggaatatc aataatcctg ggctttttta ttgaggtgat
    89521 ttatgacatt tttagattta ttcgccggga ttggtggctt tcgcttaggt atggaacaag
    89581 ccggtcacca gtgtatcggt ttttgtgaaa ttgatgaatt tgctcggagg agttataaag
    89641 caatccatga tacaaggaag gaggtggaaa tgcatgacat cacaagtgta tcagacgaat
    89701 ttgttcgatc cctcggacca gtggacatcc tttgcggcgg ctttccgtgc caagcttttt
    89761 caattgcggg aaagcggcaa ggattttccg atactcgagg tactttattc tttgaaatcg
    89821 ctcggttcgc cactcttctc caacctaagt ttctattcct tgagaacgtc cggggattgc
    89881 tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgttggat ggattggggt
    89941 atgatgtgga atggcaagtg cttaactcaa aggcctacgt tccccaaagc cgtgagcgga
    90001 tcttccttat cggacattct cgagatgcat gtaccgaaca agtatttcct atcattggat
    90061 cgtcttcaac atctgatcaa aacatcagaa acttggtaaa tatcaatcct tccaatcgag
    90121 gaatgggtgg ccaagtctat ggttcagata gcgtagcacc aaccttgacc ggtgatgaag
    90181 gaattaaaat tgccttaccg gtaaaggatg ggatttctgt agcgggtatg ttgtcgggaa
    90241 attttgaaca aggcaatagg atttatgagg taaccggaac agcccccact ttgtcaacga
    90301 aacaaggggg aacgaagatt atgattcgga aaaatgaaac agaatatcaa gaggtgaagc
    90361 caggggacag tgtgaacctg gcttttccta attccacaag tcgccgaggt cgtctgggaa
    90421 aacaaagtgt gcatacctta ctaacaggag accaacaagc ggtagtcacg gatcagtacc
    90481 agattcgaaa gttaacacct agggagtgct ggcggcttca gggctttcca gattgggctt
    90541 ttgatcgagc ttctcaagtc aattcagata gtcagttgta taaacaggcc ggaaactcag
    90601 taacagttcc ggtcattttt gatattgcta gaagattaaa gggggtaaaa gcatatgatt
    90661 tatgacgagt tgattggaga aatctactgg gtgattggca agattcaggc agatccgaaa
    90721 ttggaagaag aactgtaccg gctcaatttt gatattcgca aaaacggggt gaaagtccag
    90781 gtgattcgta tgtagtggat gaggaaacgg atgctcgaat ggagctcaat caagtcatcg
    90841 gtgaattgga acgaattgcg gaccttgcca aagaacctga tatccgccaa tacttgtttg
    90901 agataaaagc agagttggaa attgagggta tcaccgcgga gtaataggga aataacccac
    90961 tgataggcac tgaccgcggg ggatttgcga agcagaaatc ctccgtggtg ctgtgcctgt
    91021 tcatggacgg cggcagccgg ccatgatgtc tcaccccccg tatctaacag gggggtacaa
    91081 aaacacaaaa aacaagcaag taaccataaa aaatgcttgt tgtatcaagg atattaagta
    91141 atttgtatag gtgttaactc atttggaaaa gttaacacct tgaatttgga ggtgaaaact
    91201 tggcgcaacg aaatttagat tatcggttat taaaagaacg acgcaacgaa tatggcgttt
    91261 cacaaaataa attagctact gcttgcggac ttagccgccc atacctaaat cagatagaaa
    91321 acggtggcgt aatcgcctct acaaagacca tgagaaaaat ttttgatcaa ctggaaagtt
    91381 tcaatcccga tttaccttta acgttactat ttgactatgt aaggattcgc tttcccacaa
    91441 cggatgtgcg gaaaattatt caagaaattc tccatttgaa attcgagtat atgcttcatg
    91501 aagattacgc cttttactcc tatcaagaac aatatgtcat gggcgatatt gtagtgatgt
    91561 tatctcatga agaagataaa ggtgtccttt tggaattaaa aggtcgtggt tgccggcagt
    91621 ttgaaacttt tttactcgcc caaaagcgta gctggtacga cttttttgaa gattgtctaa
    91681 aagccggtgg cgtgatgaaa cgcttggatt tagcaatcaa tgatcgagtg gggctactgg
    91741 atattcctga tttaacgaaa aaatgtcaga aggaagaatg tatctccttg tttcgtacct
    91801 tcaaaagtta ccgttccggg gaactgttga aagctgatga aaaggatggt atgggcaata
    91861 ccttatatat tggcagcctc aaaagcgagg tttatttttg tttgtacgaa aaagattatg
    91921 aacaatatat caagttaggg attccattgg accagaccga aacgaaaaat cgttttgaga
    91981 ttcggttgaa aaatgaccgt gcttattatg cgattcaaga tttgttaaaa ggtcgtagca
    92041 ttgaaagtac cacgttttcc attatcaatc ggtatttgcg atttgcggat aaagtagatg
    92101 gaaaacggcg aaccaattgg cccttgaatg aacaatgggg tcgttttatt ggacggaatc
    92161 ggaaagagat tcaattaact tctgaaccga aaccgtatac cattgaacga acattaaatt
    92221 ggctaggtag acaagtcgcc cctacgtgga aaatggctaa ggagttggat cgattgaatc
    92281 aaacaacgta tatccaggat atggtacaga atgcgcgact ctcggaccgg cataaaaaga
    92341 ttttggagca acaaagtatg gcaatcgaaa acttgattgt atgaaaagag gaattttgat
    92401 ggaaattgta aaagatatgt tattggtact tggtggcagt atgcttggtg ttaccgtgat
    92461 gtgcttgatg aatgcaagtg cagcagcgga ttgagcaatg gaaaagaaag gaaagtgaca
    92521 aaatgaattt cggacaaaat ttatatcaat ggtttttaac caatgctcaa tctttagtct
    92581 tactagcgat tgtggtcatt ggtttgtttt taggatttaa acgggagttt tccaaactga
    92641 ttggcttctt ggtgattgct ttgattgcgg taggtctggt ctttaatgct tctggtgtga
    92701 aggatgtcct actcaatttg tttaaccgaa ttgtcggtgc ctgaggggga atcacaatgg
    92761 aacagatgcg tgtttatatt gcgaaccttg gaaaatacaa tgaaggtgag ttggtggggg
    92821 catggtttac cccacctgtc gattttgatg aagtgaaaga acagattgga ttaaatgacg
    92881 aatatgaaga gtacgcgatt catgattatg aactgccttt tgagattgat gagtatacgc
    92941 caattgaaga gattaatcgg ttgtgtaact tagcggagga actagaagga acgccaattg
    93001 gtgaagctgc ttctgagatt caacacgctt tcttcaattc tttcgaggaa atggtagagc
    93061 atgtggatga tattgtctat tatccagatt gcgatgatat gagtgatgtg gcgcattact
    93121 tgattgtaga agctggtgat tttggcgaag ttccagaaca attgaagccg tatattgatt
    93181 ttgatgcttg cggtcgagat ttggagattg gaggaaatta tttggttact agtcgtggag
    93241 tttttgagta tccagaatga atcaaacact atatatagtg tttgattagt atatgtaagc
    93301 aaaaaacaca atatatagag tgcaaactga agggtaatgt gatataatat ttgccgaaga
    93361 aatatttttg aaatgaggta atattatggc aaatattcaa acaagtttta ttgatttcca
    93421 taattcaatt agattggacg tagaagacaa tacgctactt aaggactata aagaccaagt
    93481 aattgatgga ttaaaagatt acttacctga tgatgtgaag tttgaaactt ttttacaggg
    93541 aagctattct gtttataccg gaataaaaag ctgtgatgaa aaaattgatt ttgacatcga
    93601 tattgcagtt gctttcgaaa ttgatcatac tgtttatgaa gatccaaggg agcctaaact
    93661 gtgggtaaaa gaggcgctag ttgaaatttt tcctaatgct caagttaatt taaaagtacc
    93721 ttgtgtaact gctacattta ctggaaaaaa gacaaaaaaa aatgtgcacg ttgatgttgc
    93781 ggtttatgct aaagaagatg aaaattattt cttagcaaaa gcaaaagaat tcagtgctcc
    93841 cgaaaaccga tgctgggagg aagcggatcc caaagtatta aaagagaaaa ttaatagtca
    93901 tgtagcagat tcagatgatc gaaaacaatt tcgaagatgt attcgctatt taaaaagatg
    93961 gaaagataac aattttaacc aagaatataa accaacagga attggactta caattaatgt
    94021 gatggatacg ttcctagtta ataagtccac tgatttttta actagaaaag ttcagtataa
    94081 tgatatggaa tgtatgaagc agattgtttc ttcgctgaaa gattcatttg tgtatgagta
    94141 cagtgaaaca gatggttggc attatcgtct acatgcatat cttccagtga agcctaacag
    94201 tgacacctat tcaaaaatga cagtgaatca gatgagtgat tttaaaaaca aactttctaa
    94261 attatatgat gatttgattt tcgctatcga tactgaagat gagtatgaag ctacaaaacg
    94321 attaaataat caatttgggg aggatttttt aattatttca gaagaagaag ttacagaaaa
    94381 aaacttaaga aatgcatttg ttacagacta tccgagtgct taggttggtg ttaaagattg
    94441 gattatactg atattttaaa agaaaagcta acagatgttg tgcgtttatt cgatggtaat
    94501 tactatgggg atttttcatt tgctacaatt ggaaagttgc gtttaaaaat tgaaatacca
    94561 aaacattttc cattggtact tcctaggatt tacattcata attttgatca tttaggactt
    94621 tacctaccac atgtagaaaa agatgggttg gtatgttttt cttcagatat aaattattta
    94681 gtagatgaag aaaattcaga attggtttta ttgcagtgtg ttaataaaac tataaaaaca
    94741 attgaggaag gtttgaaagg gttaaataaa aatgattttc gaaaagagtt tgttgctttt
    94801 tgggacaaac aagagaatca aatttcgtta ttctaccttt gtgaaccagc gttgtttatc
    94861 aagtcagttg atacaataat gtacggtaag tctttggttg ctttggataa agagaacgaa
    94921 ttacaattaa atgcagcgag tagattgttt aatggagaga aagaagatac tcatgaatta
    94981 gatgcatact atgttccaat tagaaacaag aataatatta tgcccccgaa gaataataaa
    95041 gcatggtcaa agaaggatat tttacaaata gtaaaaaaaa atgtaacagg tgcaaataaa
    95101 agattttttt taaaacttga aaaaagaaag aatagacata atgttttact tattctagat
    95161 atcccaattg aagaaaagtc aaataatgtc ataattggat ttctttttac aaaacaatct
    95221 ggatctataa aagattataa gggtacgcct aggactgtat tagttcagcg tcatgatgaa
    95281 ttatatttgg tgaatagaac gagcggagaa aactatggat tgggtgctaa tatagctatt
    95341 gttggggtag ggtcagttgg cagtaaaatt gctgaaaatc ttggtccatt aggtgtgaga
    95401 aagatgacac tcatagacaa tgattgtttt caagctgaaa atttgtatcg aaattcactt
    95461 ggtgcagata gtttaaaaaa tgcccgatct cagaataatt tttttaaagt tgatttaatg
    95521 caggaggagc tcgaaaaaaa attcccatac ctttgtgttg aagtggagtc aatgaacgtt
    95581 ttagacttaa ttagtatgca acctggtttt ttcaaggaat atgactatgt ttttgtttgc
    95641 ataggagata caatggcaag cctggcttta aataattatt tttccaaaag tagtcaaaaa
    95701 gttttctatt cttggctgga gccatatggc attgggggac acactctatt tataaattat
    95761 tctctaaatc aaaaaggttg tttcaattgt ctttatacag attcaaactc taaaaatagg
    95821 tgttcaaata gagcttctct ggtaaaagaa ggacaaattt ttgaaaggac tttagcgagt
    95881 tgccgctcaa aatttatacc atataatgct attgcctcga cggaatgtgc aattaatgca
    95941 ataaaacttt tccataaagt caatcaaaat aatatgacca atagtactct tttttcatgg
    96001 ataggagatg ggagtacttt tgagaattta ggatatcaat atagcgacag aaatactttt
    96061 cttaatggag tcaataagtt tgaagaaaga ttcttttcta aatattgcaa agtatgtggg
    96121 gagttagatg attaaatttt cgtgtgaccg tattgtcatt gaagtaaggg atgaagttct
    96181 taagatattt gaacaatatc ggcagggaaa ggatgacttg cctgaagctg gaggagttct
    96241 tcatggaaaa agacttaaga atgggagcat aataattacc gatataacaa cacctcaaat
    96301 aggagatata caaaaaagat tactctttaa aaaaaaccga gaaattcatc aacagctttc
    96361 taatgagaaa tggatagaat caaataagtt ttctattgca ttgggagaat ggcatactca
    96421 tcctgaagct gtaccaactc catcaaatgt tgataagaaa agctggagac tgaacgtgag
    96481 caagcaacat gatgatagag tttatgtttt tattatcgtt ggattttctg atttgagaat
    96541 gtggttactg tcaaaaaaat cagatattag gcaggctata aaactataaa ggagtgatta
    96601 gttgaaatca caaaagaata tttggtggtg ggggttttta gtaggaaagg taatgatacc
    96661 agcccttctc ttaatttctc taccgatgat tacttttttc tttaccaata aaaaaatgag
    96721 taaagaagcc gtgatttttt tctttggaga ccaaaaagct acctttattg aaacgttagt
    96781 aacatctctt cagcttaatt tgaattacat tttttcaatt attattgtta tatcgttatt
    96841 gaattttttt aaaaaaagaa attcaggaaa ggtgtttaac tctaatggaa atgtgtacta
    96901 caactatttt tattttgtat tttgggtagc agcaacttta cttggttatg ataaaattca
    96961 gattgcagga attcctatac acatgcaata taagttggtt ttgagtggaa ttttttctga
    97021 ggtgttgcca gatatatacg atgatcatta cgacagtgat ggaacatgta aagtaagcat
    97081 tgaaaaagaa aatttcgatg acatcgatgg atatgactct gtgaacttac tgattattga
    97141 tacttacgat ataaaaatga gtgaattatc tatggaaaat caaacatatc ctacgataat
    97201 tgttagaggg aattcaatag atggagttcg taaagttaat agatctttaa tcttggaaat
    97261 aaaaaaaaca atggatgaaa ttcaaaaaag tgactttaaa aaagtatttg ttgcttcaac
    97321 atctaatcca aaaaatagta tcaatattat taattcatcg tttagatttt tcggtcgatc
    97381 acgtaggttt aaactttatg ttcttcaaaa ggattatgca agcaatggaa agtatagtaa
    97441 gaaatataga atttttattt aagaaggaat ttttccttct ttttattttg gaggaaacta
    97501 tgaaaaaaat aaaaagctac accagtattt gggcagttga aaaagtcatt tatgcgatta
    97561 atgactttca gctgcctttt cctgtgacct ttaaccagat ggcttggttt gttttatcct
    97621 tgatgtttgt cattgtattt gcccatgtgc ctccgctatc catgatagag ggggcttttt
    97681 taaaatactt gggcattccg atagcggtca cttggttcat gtcgcaaaag acctttgacg
    97741 gtaagaaacc attgggattt ttacgatcct ttatcagcta tcacttacgg tttaaggtca
    97801 cttttgcagg aaagaaagtt aaagaacaga agaaacgatg ggacgaacca atcaccttgg
    97861 taaggagtgt gaattatgta cccgattaag tatattgaaa ataatcttgt ctttaaccaa
    97921 gaaggcgagt gttttgctta ctatgaatta gtgccttaca attactcctt tttgtcacct
    97981 gaacagaagt atcaagtaca tgataacttt cgccaattaa ttgcgcaaaa tcgtgaaggt
    98041 aagatccatg ccttacaaat tgccacagaa agtagtattc gtgccacaca agaacgttcg
    98101 aaaaaggaaa ttaccggtag actcaaagaa gtagcaaagc aacgaattga tttacagaca
    98161 gatgcattag tatccatgat tggcgatagt cagattgatt atcgcttctt tattggcttt
    98221 aaactgattt cgacagatga agaggtcaat ctaaagaatc tcaggaaatc tttcttttct
    98281 ggacttcaag aatttgtcta tggagtgaat catcatttga tgggtgattt tgtttcctta
    98341 tccaatgaag aaattcgtcg ctataccaag cttgaaaaac taatggaaag taaattggct
    98401 cgaagattta aagtcaaacg agtaacgccg agtgacttga cgtatttaat tgaacatatc
    98461 tatggtgaaa aaggcacacc gtttgaagaa tatgaattcc agctaccaaa gaaaaagctg
    98521 aaatcagaaa cgctggtgaa acgttacgac ttattacgtc ccactcgttg tttgattgaa
    98581 gaaaagcctc ggtacttacg gatggaacat gaaaatcatg agtcttatgt agcgtactta
    98641 acaattaata ccattgtagg ggaaatggag tttccttcct cggaactttt ctattaccag
    98701 caacaacaat ttacgtttcc gattgatact tcaatgaatg tggaaattgt gacgaataaa
    98761 aaggccctag ctaccgttcg taataagaaa aaggaattaa aagatttaga caaccatgcc
    98821 tatcaatctg ataatgaaac gaactccaat gtattagatg ctttggattc cgtggatgaa
    98881 ttggaaacca ccttggatca gtcgaaagaa tccatgtata aactaagcta tgtggttcga
    98941 gtgagtgcta aatcagtgga tgaattaaaa cgccgctgtg atgaagtgct cgatttttat
    99001 gacgatacca atgtaaaact agtccggccc tttggcgata tgatgggtct acacaaagaa
    99061 ttcttaccct caagtaagcg atacatgaat gactatattc aatatgttac gtctgacttt
    99121 ttggcgggac ttggttttgg ggctactcaa atgctaggag aattagaagg catttacttt
    99181 ggctataacg tagatacggg gcgcaatgtt tacctgaaac cggcattggc atcacaaggg
    99241 gtcaaaggct cagtaaccaa tgctttggca tctgctttcc ttggttcgtt aggtgggggg
    99301 aaatctttta gcaacaatct cttagtctat tatgccgtcc tttttggtgg gcaagctgtg
    99361 attgtcgatc caaaaggaga aagaggcggt tggaaagaaa cgttgccaga aattgcggat
    99421 gaaattaata ttcttaactt gaccagtaag ccagaaaatc aaggcttact tgatccctat
    99481 gtgattatga agaaaaagaa agactcagaa agtttggcga ttgatatcct gaccttttta
    99541 acagggattt ccagtcggga tggggagaag ttccctgtgt tacgcaaagc cattcgaacg
    99601 gttacccaaa gtgatcagcg aggattatta caagtaatcg ttgaattgcg taaagaggac
    99661 tcaccagtcg cggaaaatat cgcggatcac atcgagtcct ttaccgatta tgattttgcc
    99721 catttgcttt tctcagatgg ttcgattacc cagtcgatta gtttagataa gcagttaaat
    99781 atcattcaag tagcggattt ggttttacca gatgcagaaa ccacttttga agaatatacc
    99841 accatggaat tgctgagtgt ttccatgcta attgttattt ccacttttgc cttggacttt
    99901 atccatagca accgagaaat tttcaagatt gtggacctgg atgaggcttg gagttttctt
    99961 caagtagctc aaggaaaaac gttatcgaat aaattagtgc gtgctggtcg ggccatgaat
   100021 gccggtgttt acttcgtcac gcaaaacagt gatgacttgt tggatgaacg tttgaagaat
   100081 aatattgggc taaagtttgc atttcgcagt accgatattc atgaaatcaa gaaaaccttg
   100141 gaattctttg gattagataa agaagatgaa agtaatcaaa aatgcatgcg agatttggaa
   100201 aatgggcaat gcttgatgca ggatttgtat ggtcgagtag gtgtggtcca agttcatcca
   100261 gtctttgaag aacttttcca tgcttttgat acccgtccgc cagttcaaga gagaatggag
   100321 tgaggagatg aaaaagaaac tttttcgtat ctttggaatt gttttaatca gtttggtcac
   100381 tctgataata cttttgagct tagttggaac ggtagctgag gcaacaggtc tggttgatga
   100441 tacagttgga gccggaaatc tttattccca atattcactt ggtaactatc agttggattt
   100501 ctttgtggat agttcgtggg attggctgcc ttggaattgg ggtgatggcc tagggaagag
   100561 cgtaatgtat ggactttatg ccattactaa ttttatttgg acagttagtc tctacttatc
   100621 aaatgccacg ggctacgtgg ttcaagaagc ctataagctg gatttcattt cagatacggc
   100681 tgaaagtatt gggaagaata tccaaacctt agccggcatt accgaaaatg gactccagac
   100741 ctcgggcttt tactttgggt ttctattatt gatgatttta gctctaggag tttatgtggc
   100801 gtatacagga
//
LOCUS       DACDRN010000006        71038 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDRN010000006 DACDRN010000000
VERSION     DACDRN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.006"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1034)
                     /locus_tag="IWJ80_000579"
     CDS             complement(<1..1034)
                     /locus_tag="IWJ80_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9460344.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLV"
     gene            complement(1063..1380)
                     /locus_tag="IWJ80_000580"
     CDS             complement(1063..1380)
                     /locus_tag="IWJ80_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9460345.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(1406..1726)
                     /locus_tag="IWJ80_000581"
     CDS             complement(1406..1726)
                     /locus_tag="IWJ80_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9460346.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(2190..3605)
                     /locus_tag="IWJ80_000582"
     CDS             complement(2190..3605)
                     /locus_tag="IWJ80_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9460347.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(3622..4938)
                     /locus_tag="IWJ80_000583"
     CDS             complement(3622..4938)
                     /locus_tag="IWJ80_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9460348.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(5088..5786)
                     /locus_tag="IWJ80_000584"
     CDS             complement(5088..5786)
                     /locus_tag="IWJ80_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP9460349.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(6075..7064)
                     /locus_tag="IWJ80_000585"
     CDS             complement(6075..7064)
                     /locus_tag="IWJ80_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP9460350.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(7144..7977)
                     /gene="msrA"
                     /locus_tag="IWJ80_000586"
     CDS             complement(7144..7977)
                     /gene="msrA"
                     /locus_tag="IWJ80_000586"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9460351.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(8152..9786)
                     /locus_tag="IWJ80_000587"
     CDS             complement(8152..9786)
                     /locus_tag="IWJ80_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460352.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(9773..11620)
                     /locus_tag="IWJ80_000588"
     CDS             complement(9773..11620)
                     /locus_tag="IWJ80_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460353.1"
                     /translation="MFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKDNSSILKI
                     ELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRIKGLSS
                     IFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNICTILI
                     SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAYGLI
                     FNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLV
                     KAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTM
                     GLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKAPISLLQFPWR
                     ILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHG
                     HIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNI
                     TYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKY
                     TPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            11607..13244
                     /locus_tag="IWJ80_000589"
     CDS             11607..13244
                     /locus_tag="IWJ80_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9460354.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(13389..14129)
                     /gene="truA"
                     /locus_tag="IWJ80_000590"
     CDS             complement(13389..14129)
                     /gene="truA"
                     /locus_tag="IWJ80_000590"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9460355.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(14320..14955)
                     /locus_tag="IWJ80_000591"
     CDS             complement(14320..14955)
                     /locus_tag="IWJ80_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9460356.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(15102..16343)
                     /gene="sstT"
                     /locus_tag="IWJ80_000592"
     CDS             complement(15102..16343)
                     /gene="sstT"
                     /locus_tag="IWJ80_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP9460357.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            16541..17539
                     /locus_tag="IWJ80_000593"
     CDS             16541..17539
                     /locus_tag="IWJ80_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460358.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            17552..19570
                     /locus_tag="IWJ80_000594"
     CDS             17552..19570
                     /locus_tag="IWJ80_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP9460359.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(19630..20346)
                     /locus_tag="IWJ80_000595"
     CDS             complement(19630..20346)
                     /locus_tag="IWJ80_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9460360.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(20573..21115)
                     /locus_tag="IWJ80_000596"
     CDS             complement(20573..21115)
                     /locus_tag="IWJ80_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9460361.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(21120..21347)
                     /locus_tag="IWJ80_000597"
     CDS             complement(21120..21347)
                     /locus_tag="IWJ80_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP9460362.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(21426..23306)
                     /locus_tag="IWJ80_000598"
     CDS             complement(21426..23306)
                     /locus_tag="IWJ80_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9460363.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(23495..24109)
                     /locus_tag="IWJ80_000599"
     CDS             complement(23495..24109)
                     /locus_tag="IWJ80_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9460364.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            24251..24811
                     /locus_tag="IWJ80_000600"
     CDS             24251..24811
                     /locus_tag="IWJ80_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9460365.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(25116..25430)
                     /locus_tag="IWJ80_000601"
                     /pseudo
     CDS             complement(25116..25430)
                     /locus_tag="IWJ80_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(25684..27319)
                     /locus_tag="IWJ80_000602"
                     /pseudo
     CDS             complement(25684..27319)
                     /locus_tag="IWJ80_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27642..28121)
                     /gene="rlmH"
                     /locus_tag="IWJ80_000603"
     CDS             complement(27642..28121)
                     /gene="rlmH"
                     /locus_tag="IWJ80_000603"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP9460366.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            28549..29838
                     /locus_tag="IWJ80_000604"
     CDS             28549..29838
                     /locus_tag="IWJ80_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9460367.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(29877..30482)
                     /locus_tag="IWJ80_000605"
     CDS             complement(29877..30482)
                     /locus_tag="IWJ80_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP9460368.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(30687..31151)
                     /locus_tag="IWJ80_000606"
     CDS             complement(30687..31151)
                     /locus_tag="IWJ80_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9460369.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(31173..31493)
                     /locus_tag="IWJ80_000607"
     CDS             complement(31173..31493)
                     /locus_tag="IWJ80_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9460370.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(31637..32716)
                     /gene="pepA"
                     /locus_tag="IWJ80_000608"
     CDS             complement(31637..32716)
                     /gene="pepA"
                     /locus_tag="IWJ80_000608"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP9460371.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            32916..33266
                     /locus_tag="IWJ80_000609"
     CDS             32916..33266
                     /locus_tag="IWJ80_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9460372.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            33489..34436
                     /locus_tag="IWJ80_000610"
     CDS             33489..34436
                     /locus_tag="IWJ80_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9460373.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(34493..35911)
                     /gene="pepV"
                     /locus_tag="IWJ80_000611"
     CDS             complement(34493..35911)
                     /gene="pepV"
                     /locus_tag="IWJ80_000611"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP9460374.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            36060..36900
                     /locus_tag="IWJ80_000612"
                     /pseudo
     CDS             36060..36900
                     /locus_tag="IWJ80_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
     gene            36904..37344
                     /locus_tag="IWJ80_000613"
     CDS             36904..37344
                     /locus_tag="IWJ80_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP9460375.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            37394..37588
                     /locus_tag="IWJ80_000614"
     CDS             37394..37588
                     /locus_tag="IWJ80_000614"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460376.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            37724..38410
                     /locus_tag="IWJ80_000615"
     CDS             37724..38410
                     /locus_tag="IWJ80_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9460377.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(38818..39507)
                     /locus_tag="IWJ80_000616"
     CDS             complement(38818..39507)
                     /locus_tag="IWJ80_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460378.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(39482..41611)
                     /locus_tag="IWJ80_000617"
     CDS             complement(39482..41611)
                     /locus_tag="IWJ80_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP9460379.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(41659..42996)
                     /locus_tag="IWJ80_000618"
     CDS             complement(41659..42996)
                     /locus_tag="IWJ80_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP9460380.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(43248..43607)
                     /gene="rplT"
                     /locus_tag="IWJ80_000619"
     CDS             complement(43248..43607)
                     /gene="rplT"
                     /locus_tag="IWJ80_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP9460381.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(43660..43860)
                     /gene="rpmI"
                     /locus_tag="IWJ80_000620"
     CDS             complement(43660..43860)
                     /gene="rpmI"
                     /locus_tag="IWJ80_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP9460382.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(43932..44456)
                     /gene="infC"
                     /locus_tag="IWJ80_000621"
     CDS             complement(43932..44456)
                     /gene="infC"
                     /locus_tag="IWJ80_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP9460383.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            44776..45408
                     /locus_tag="IWJ80_000622"
     CDS             44776..45408
                     /locus_tag="IWJ80_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9460384.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(45410..46315)
                     /locus_tag="IWJ80_000623"
     CDS             complement(45410..46315)
                     /locus_tag="IWJ80_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9460385.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(46400..47053)
                     /locus_tag="IWJ80_000624"
     CDS             complement(46400..47053)
                     /locus_tag="IWJ80_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9460386.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            47375..48319
                     /gene="mvk"
                     /locus_tag="IWJ80_000625"
     CDS             47375..48319
                     /gene="mvk"
                     /locus_tag="IWJ80_000625"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP9460387.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            48312..49289
                     /gene="mvaD"
                     /locus_tag="IWJ80_000626"
     CDS             48312..49289
                     /gene="mvaD"
                     /locus_tag="IWJ80_000626"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP9460388.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNFKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            49302..50387
                     /locus_tag="IWJ80_000627"
     CDS             49302..50387
                     /locus_tag="IWJ80_000627"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP9460389.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            50384..51448
                     /locus_tag="IWJ80_000628"
     CDS             50384..51448
                     /locus_tag="IWJ80_000628"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP9460390.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(51697..52500)
                     /locus_tag="IWJ80_000629"
     CDS             complement(51697..52500)
                     /locus_tag="IWJ80_000629"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP9460391.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(52520..53362)
                     /gene="kduI"
                     /locus_tag="IWJ80_000630"
     CDS             complement(52520..53362)
                     /gene="kduI"
                     /locus_tag="IWJ80_000630"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP9460392.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(53378..54385)
                     /locus_tag="IWJ80_000631"
     CDS             complement(53378..54385)
                     /locus_tag="IWJ80_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP9460393.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(54392..55036)
                     /locus_tag="IWJ80_000632"
     CDS             complement(54392..55036)
                     /locus_tag="IWJ80_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9460394.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(55136..55894)
                     /locus_tag="IWJ80_000633"
     CDS             complement(55136..55894)
                     /locus_tag="IWJ80_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP9460395.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(56021..57172)
                     /gene="fucO"
                     /locus_tag="IWJ80_000634"
     CDS             complement(56021..57172)
                     /gene="fucO"
                     /locus_tag="IWJ80_000634"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP9460396.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(57254..57565)
                     /gene="rhaM"
                     /locus_tag="IWJ80_000635"
     CDS             complement(57254..57565)
                     /gene="rhaM"
                     /locus_tag="IWJ80_000635"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP9460397.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(57567..58412)
                     /gene="rhaD"
                     /locus_tag="IWJ80_000636"
     CDS             complement(57567..58412)
                     /gene="rhaD"
                     /locus_tag="IWJ80_000636"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP9460398.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(58426..59682)
                     /gene="rhaA"
                     /locus_tag="IWJ80_000637"
     CDS             complement(58426..59682)
                     /gene="rhaA"
                     /locus_tag="IWJ80_000637"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP9460399.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(59679..61139)
                     /gene="rhaB"
                     /locus_tag="IWJ80_000638"
     CDS             complement(59679..61139)
                     /gene="rhaB"
                     /locus_tag="IWJ80_000638"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP9460400.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            61292..62161
                     /locus_tag="IWJ80_000639"
     CDS             61292..62161
                     /locus_tag="IWJ80_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9460401.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(62287..63186)
                     /locus_tag="IWJ80_000640"
     CDS             complement(62287..63186)
                     /locus_tag="IWJ80_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9460402.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(63351..64175)
                     /locus_tag="IWJ80_000641"
     CDS             complement(63351..64175)
                     /locus_tag="IWJ80_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9460403.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(64186..65481)
                     /locus_tag="IWJ80_000642"
     CDS             complement(64186..65481)
                     /locus_tag="IWJ80_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP9460404.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(65497..65976)
                     /locus_tag="IWJ80_000643"
     CDS             complement(65497..65976)
                     /locus_tag="IWJ80_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP9460405.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(65982..67010)
                     /locus_tag="IWJ80_000644"
     CDS             complement(65982..67010)
                     /locus_tag="IWJ80_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP9460406.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(67027..68133)
                     /locus_tag="IWJ80_000645"
     CDS             complement(67027..68133)
                     /locus_tag="IWJ80_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9460407.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(68362..70959)
                     /gene="adhE"
                     /locus_tag="IWJ80_000646"
                     /gene_synonym="adhC"
     CDS             complement(68362..70959)
                     /gene="adhE"
                     /locus_tag="IWJ80_000646"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP9460408.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT        21137 a        14674 c        12268 g        22959 t
ORIGIN      
        1 actaagacca ttggtgtcaa aataaatgga atgaacatga tcgggttcaa cacgataggt
       61 aacccaaaca tcgttggttc attaatattg aagacacctg gcgcaatcga caagtttgtt
      121 acaaccatat atggtttgtt tttacgtcct actaaataaa tagctaacag taaacctaaa
      181 gttgcacctg ttcctcctaa gttgacaaag ctgtcaaaga aaggtttgtt cacgataaac
      241 ggtgcgctat gtccagctgc gattgcatta acgtttgctt caatggctgg cgcattgatt
      301 gtttgcatca aaggatcgac catattcgct ccgtgtaaac caaagaacca taagaatggt
      361 gtgataaatg caagcagtaa ggcagatgga taagagtttg caagtcccat aaatggttct
      421 tggaccactg tatagaaaga acctacgata tcatcaacac cgaatgcagc aaaaattgca
      481 gcaactaaag caaacgcaga gatagtgatc attgctggta gtaatgaagc aaatgctttt
      541 gcaactgccg gtggcacacc atcaggcatt ttgatgatca atttttcatt tccgcttaaa
      601 cgtacaaaga tttctgttga aataatccca acaataatag caataaacaa accgtttgag
      661 ctcataccta atgctccgcc taaagcgaag aaagagccta aagaaatcac acctgcggaa
      721 acaccgtctt ttcggtagcc gttagctaaa tgataagcaa gcaagaaagc aatcaagaca
      781 gaaagaatgg caaatgtacc gttccaagca gcagccccga atcctttcca tgattcacca
      841 ccaaaaattg agttcatcaa ttcttggaac ccgggaatcg gtaagttatt gatcattact
      901 gctaatgctc ctaagatcat taacggcata ctcaccataa atgagtcccg aatcgcaact
      961 aaatgccgtt gtgcaccaat ttttgatgct acaggtataa aatgtttctc cataaatgca
     1021 acaaatttat ccataaatct tccctctttt ctttactgta gattacgcgt tcaatcgacg
     1081 atacagatca atgatctctt ttgctaaatc tgtaaaagct atagatgtca tcagatgatc
     1141 ttgactgtgg accgtcaaaa gcgtgacttc aacgtgattt ccttgtgctt cttgtgtcag
     1201 catctctgtt tgagagtgat gtgcttggac taaagattcc tctgcatctt tgattttttc
     1261 atctgctaaa gtgaaatcac cttttttcgc agcagcaatc gcttccattg catcgctttt
     1321 tgcatttccg ccaaacatga tcagacccat gatggcttct agattttttt gatcttccat
     1381 ttattgtatc ctccgataat ttttattatt tgtttattag tttttctgct tggtcgagta
     1441 ctttttcacc attcatcatg ccatagtcag ccatattgat tacatctaaa ggaatgcctt
     1501 taggtgctag cttttcagca aattgatttt gcataaagcg tacttgcggt cctaaaagta
     1561 aaacgtctac atttttagat tctagttgat tatccgcatc tgaagcagat acagcaaaga
     1621 tgtctgcttc cattcctttt gcttctgctg ccttttgcat ttttgtgact aggagacttg
     1681 tactcattcc agctgaacat acaagcatga ttgttttctt ttccatttta aacacgttcc
     1741 tttctatact tacacttgta tataagcaag tagcgtgcca actttaaaag cgctttaaaa
     1801 agttgatatc atcacattta aggtctatac caagttgcat acacactaaa aaagaaagcg
     1861 cacactaaac acacaaatta atttttatta ccttttttct tttaacgtgc acaaaaaaag
     1921 ttgtgacaca aaacttgtca caactttttt caaataaacg gtattcaaaa gcttgcatct
     1981 gcggtaataa gtccaaccaa gttaaaaaca taagatgttg tttttacttt gctattctta
     2041 ttacttgatg caggacagct ttttcacaac ctctcaataa acggtgttca atcagctgac
     2101 cctgggtatt agctcaaaac gctggattca caacctcttt tatttcaaat aaccagtaaa
     2161 tacaatgaac atctacaaaa aacttctatt tatttataga tttcgtcagg caaatccaat
     2221 tctctcttat caatcaaagt ctgataccac gaagcagatt ttttcaatgt tctgcttcgt
     2281 tcatcgttca attcgattcg aactaatcca tatcgattct taaatgcatt cattggtgac
     2341 acacagtcag tgaatgccca taacatatag ccttcgcaat ttgcgccttc ttctgttgct
     2401 ttgatcaagt aatgcaaatg ctgtccgata aattcgatac ggtaatcatc ttgcacgatt
     2461 cctttctcat ccatatactg ttcttcatat tctcttccca taccgttttc tgttatcagc
     2521 caagggatat tatgataatt ttcttttaga tacatggcta tatcatacat gattttcgga
     2581 tagatttccc atcccctgga attattcatt ttcttcccag gtaaatcaaa gacttcataa
     2641 tatcgttcag gatggaaagg tgtctcttca ttccattgat aacgaggggc gcgaacgcgt
     2701 ttaggataat attgattgat tccaaggaaa tctactgtat gatgctgaat cgtttctaaa
     2761 tcctctgttg caggatcgaa ataaatcgat tgtttttgac acaactccag gaactcttct
     2821 gtgtagtatc cttttaccat cggatcaaaa aatactcgat tataaaacaa atcatacata
     2881 taagcagctt tttggtcttc tggagaattg gatcgagcat agaccatttc aggatttaaa
     2941 atacagccaa tccttccacc ataatttccg ctgcggtaaa tctctactgc tttagccgtt
     3001 gctaaaactt tatgataatt ccacatcatc cacttttttg tgttttgttc atgcggccag
     3061 cgaacagcat ccaaatagca acgcgtttgg ggaacgattg gttcgttgaa tgtaaaccag
     3121 ttttttaccc tatcaccgta acgatcaaag gcaatttgag catacttagc ataaaaatca
     3181 actacttttc ttgaagacca gccatcatat ttttccaaaa gaaaagctgg gacttcataa
     3241 tgttccaaac aaatcatcgg ttctacgtct gcttcgatca aagagtcgat catttcactt
     3301 atatgctgag catattcttc atctactata cagttctcat aatcagtaaa aaaacgagcc
     3361 caattgatag aagtccgata atgtgtcaga tggatggatt tcatcagatc cacatcctgc
     3421 tggtattgtt ccataaaatt agttgctacg gctgggccat acccattatg ccaaacaaaa
     3481 tgttcatttt tataccagct atctataaac gaatcctgtc cttctttttt tcctttccaa
     3541 ccttccgttt gccaagcaga agatgcagca ccaagaataa aattttttgg aatagtaatt
     3601 ttcatgattt cctcctgttt tttagatccc atcattttcc acacgatttg ctacttttac
     3661 aaatggcaga tagatcaaaa tagaaacaat aatacaaaca agttgcgtaa taactgcacc
     3721 gatatttcct gctgttgcca gataagcatt gacgatcggc ggcattggcc aagggaccat
     3781 tacaactgct ttcgccgcaa atcctaaagc ggttgcaaaa taaccaatcg ttccagtgat
     3841 aagaggcgtt aagataaacg gaatagctaa aattggattc agcatgatcg gcataccaaa
     3901 aataactggt tcattgatgt tgaagatccc aggagcaagg gataacttgg tgatttgttt
     3961 catatcttcc cgttttccaa ctaacaatac tgcaatcagt aaaccgatcg tgactccaga
     4021 cccgcccata ctcatataca tatcccaaaa tggcatagta ataatgttcg gtacttcttt
     4081 tccagcttca aaagcttccg tatttacagc aattgccgct aaaagcaacg gttcccgtat
     4141 cggcttcacc atttgatttc catggatacc aatcacccaa aagacttgcg caataaacat
     4201 caatagcaaa atacccggta acccttgtac gacaccttct aatgggcgtt gtacgacatt
     4261 gtaaatgata tcataaaggt acattcctgt aagttctttg atgataaatc ccgcagccgc
     4321 gatacccgat acagtcagaa tcgtcgggaa taaagcagaa aaactagcag aaacattcgt
     4381 tggcacggta tcaggcattc tcagctgtag tttcttctgt ttcctcaacc agcaataaag
     4441 ttcaatagaa aggatggaga ccaacattcc caaaaataac cctttcgtat ctgtatattg
     4501 tttagccaat acgttttcta ctataacagt ctgattttca ttcactaatt cataaacagt
     4561 tggattaaca gataaataag acatcactgc gatgatacca gggaatacac catccacttt
     4621 attcagcttg gctaattcga tccctatcag aaaaacagca tagatggtca agaaacttag
     4681 tgtgacatag ctgatggcag atgcgatagg ctttaattct tctaaaaaac gcaagaaact
     4741 tatctgtgct aagccatttt ccgagtcaaa gaccattgct gaaaacaatg tgccaaaagc
     4801 acccgtaata attaccggaa tcaatgcagc aaatgcgttt tttatcacca tgatatacct
     4861 taaattattg attctcgttg ccacgttccc taaaacattg gctaatttat ccataaaact
     4921 cattccagat cccctcattc ctgcttcttc tccttgacgt catctctttt tgtctattag
     4981 tcagcgctta catttcgatg aacagaaact atttactcct cttttttacc aaatataaaa
     5041 caaaaattca tctgatgtat ttatcataaa atacatcaga tgaactgtca attagtttct
     5101 ttttctccta ttattttctg taattctctc cgatttattg ccatatggac agtcattgca
     5161 tcaaacgcag caagtgcatt ccgcctttcg atggcctgaa cgatctctgt atgaatatcg
     5221 atcgtctcga ttttcagatg ctggatatca taatttttat taaacaaagc aattgattca
     5281 ttaatgattg ggataatatg cagcaaggct acgtttccgc tagcttctgc aatcatcgta
     5341 tgaaaatcga tgtctaattg aacatgttct tgtgtgcctt ctcgaaaaga agtttcaatc
     5401 ttttgcgtta attttttcat ttggacgatt tgattcttcg ttgcatattc cgctgccaaa
     5461 cctgccatct taggctccaa taaatatctc agttcaaata agtctcgtat catccgatcc
     5521 tgatcttcaa tcaaagaaaa accgagtggg tcattggcaa tccctcgctt atcactgaca
     5581 aatgtccccg ctccttgccg aacttcaagg atattcctgg aagctagagc ccgcacagct
     5641 tctcgcaccg tgcttcgccc cacctctaaa taatcggaca attcatattc attcggcagt
     5701 ttatctccta cttgcaactg catttccttt aaatatctaa ggatcttgtc agtagttact
     5761 tctactaggg ttttattttc ttccaaacca catacctcct tttcatctga tgtatttttt
     5821 cgtaattatt ataacacgtt tgaatcatct gatgattttt tgtatacaaa attgattaat
     5881 ccgttcatag acatacaaac gaagaagata tttaccataa aaaagactag gataaaattc
     5941 actcctagcc ttcgatcatt tttattttga acgacaaaaa atatgatgtt caataacaga
     6001 cgatcttcat tcgattcttg atgtcatctt ttgttttttt taatatatgg ataaaaagtt
     6061 tgattacatc gtttctattc tagaatttgg tcgattctca attcttcttc tggtgagaaa
     6121 tctaaattat ccatcattcg aacactttct tgtaactgac ttaaccgact tgctccgacc
     6181 agtacacttg taacagattt ttgtcgcaaa ttccatgcaa gggccatttg cgctaatgat
     6241 tgtcctcgtg atacggcgat tgtttgcagt gctttgattt tttccagagt tgatccaact
     6301 tgttcttctg aaaggaatgg tatttcctta cgatgtgctc tagaatcttc aggaatacca
     6361 tgtagataac gatttgtcag caacccttga gcaagcggac tgaacgtgat tgcccctagc
     6421 ccttcttctt ctaatactgg agatagatcg tcttctagta gtgcacgaga aaacatattg
     6481 tagcgcatct gatgaataat gaacggtgct tcctttcttt ttaaaatttc agtcattttt
     6541 tttgtatctt ctccattata gttggatata ccaatatata atgcttttcc ttgtcttaca
     6601 agttgcatca aagcttctgc tgtttcttcc aaaggtgtat cagggtctgg gcgatgatga
     6661 tagaagatgt caacataatc tagccccatt ctttggagac tttggtcaca acttgaaatg
     6721 atggatttac gagagcccca ttccccatat ggcccttccc acatatgata acctgctttg
     6781 ctagaaataa tcagttcatc acggtaggct tgaaaatctt ctctaaaaat acgcccaaag
     6841 tttttctctg cagctccagc agggcctcca taattattcg caaggtcgaa atgggtaatt
     6901 cccatgtcga atgcgcctct taaaatctct ctttgattat ggataggatc ataatcacca
     6961 aaattttgcc ataatccgag agataaaatc ggtaatttca aacctgaatg accggattta
     7021 cgatattcca ttttttcata acgtgcacta tctgcaatat acaatttttc tcctcctcat
     7081 caaatctttt taaaacgctt tcttttttct atttttagtt tactacattt taaagaaaag
     7141 gagttaatca aaatgatttt ttcgtatatt tttttgccat ttgtttttta ctcgttgata
     7201 gatcagaaat tgattacgtg cttgctggat ttttttataa cgtttcggtt gttttttgta
     7261 aaattgctga tgatagtttt ctgctggcca aaacgtaccg gctggttcga tttttgtgac
     7321 gattggttgg tggaaagttc ctgaactgtc tagtttctgt tttgattgtt ccgctaattc
     7381 tttttgtcgt tcgacttgat aaaaaatcac tggacgatat tgcttccctc tatcttgaaa
     7441 ttgtccaaat gtatctgtgg gatcagtaat ttgccaatag attgtcagca actcgctata
     7501 acttatttct cttgtgtcat aaatgatctc cacagcttcc acatgacctg tgtaaccacc
     7561 acttacttga tcataatttg gtttttcaac atgtccgccc gtgtatcctg acaaaacaga
     7621 taggatccct tttttcgatt caaacggctc gaccatacac cagaaacatc cccctgcaaa
     7681 tactgcacgt tcttgatagt cagcatcttg catggcatgc ttggaaaagt catatttttg
     7741 ctccttatca aatccttccg taatcaataa ataaaagtcc attacatcag gcgtcagatt
     7801 cattcggata gctaatggac gtagtgttgc ttctagtttt gataactgtt ctttaacgga
     7861 tatattttct cttatgtcat tctttgtctg aatcaatact tttcgttccc aatctcgtgt
     7921 attcggattc aaaatcagat tataaagtgt ttgaatgaat atttgttcct catccataga
     7981 ttctcctcct ttccgaagaa gacaagctag tgactttagc tgtaatttat cataaagtga
     8041 ggaaataagc gattacttca tccccctaca gatttttcgt ataaaaaaga gagaatcctt
     8101 atcgacagat tctccttttt aatcaattta ttttataaat gaaaagttaa tttacctttt
     8161 atggaaaact attttaaaaa ataaacataa atatgcagtt aatgtaacaa gataagaaat
     8221 aatagtcgtt actgtataac ttgtgcttac tttaatatca gcaactcctt tgactccgct
     8281 tatttgtacc aatccattag cattcacctg ctcctttaca ggttgatgat ttatttctac
     8341 attcgttcct aaataagcat atacaggaag tgtgattact tggctcttct cattagttac
     8401 tctatatatg atagaactac cagttgtttt ataaggaagg tttacccatt gactgccaat
     8461 ctttgcttcg tgttttatta tatgctcctt attctccaat gaagcgatag gagaataatc
     8521 atataaaaaa taatccattc ctttagtggt atacgcctct actgtttctt tgtttacaac
     8581 aatatgtccc ggctgagaaa ttattttttg attgaaattt tttttactag aaacagttaa
     8641 agtgattaat aacaaaacag tcataaataa ataaaatagt tctcttgtct tattgtatct
     8701 tctatttttg aaaaatatca tcgttaatat cagagaagag aatattattt gcaagcttaa
     8761 gatccgccaa ggaaattgta acaatttggc tggcgtattt tgcagaagat accatggcac
     8821 caaactagtt gtagctagcc aagtcaaaca cgtaccataa taaatctttt tttctatgtt
     8881 tgttaatttt ttaaataaaa tcaggggaat acataaagat aacagaccaa ctagtcccat
     8941 tgagtaagta gttaagtcgt tgttcatagc ttcagtcaga ctatcgaaca cattagatgc
     9001 atgagcatat aaatttgtca cgctaggttt gttgattttt tggtagagca tttgttcaaa
     9061 catcggatac caaaaagcac tcgtcagaac caatgtcatt cctgccgcac taaataattt
     9121 tttaaatata gcaataaaat ctgcattctt gaaaccttta gaaaccaaac gaataaaaat
     9181 aaaaatgata attaatgctg tactcattaa tgttgatagt acatggctgt acactagcaa
     9241 actcatactc aatgctagct ttacagacgg atacttttct tcttttccag agatgatttg
     9301 ataagcataa aataatacag ggataacaag tgttattgcg atagcttcgg caattgcgga
     9361 acgatagtag atgtctactg tccgataagc agagaaaata tagagacaac taaataaaaa
     9421 tgccgctttt ttttgtttta aaaaccgctc gccataatga taagctacga gtgttgtaca
     9481 caagttcaat aaccaaacgt aaataatata gcttacaatc aaattttttg tcatccaata
     9541 taacattaca gctggaaaaa aagtcagaaa gggataaaaa taatttacac catatccagt
     9601 ataattgaat gtctcataat taatcggact ggtaaaaata gtcgataacc ccttgattcg
     9661 agataagtga aaagaaagat ctccagtggt ataaatactc tgtgtatttt tgaaaaagat
     9721 gaaagaatat atactactaa ctataattaa tccactcaat attactactt ttttatttat
     9781 tttcattctt cttgtttatc tcctttttag tcacatatcg agaatctttc aacttattag
     9841 gtgcaaatcg tttaaaactt gaatacacca ctaaacaata agtcagaata ctgatccata
     9901 aagccatgag agcagttgga gtatattttg ttgtaatttc tatcttattt tctccagcat
     9961 ttacatccac attcactaag ccatgttttt tataagagtc atttacgact ttaccgttta
    10021 cttttacctg tgtacctaaa taagcatata ctggtactgt cactttttgc tttttgttgc
    10081 ttgccactgt gtaagtaatg ttattatctg cagctttata cttccctttt tcccacgatt
    10141 ctcctacttt tacttcatgt ttttctaagt gaggcctttc ttttatcgct tctgttgggg
    10201 cgtaatcgta taatcctccc atatctgatg ttgtataacg tgggagtgtt tcttcattga
    10261 taacaatatg accgtgtttt gattggataa cttttgcgta attttctttt gctgctatag
    10321 acgtgacgag tagaaacacc acagtagcgc caatgattaa ccatgaatat tttttattca
    10381 tagtcttttt cataaaaaca agcgttatga tcattgaaga aaaaaagatt tggataccta
    10441 agatacgcca aggaaactgg agtaagctta tcggtgcttt ttgaaacaaa aaccatggaa
    10501 ataaattagt tacgcatacc catgtagaaa ctacgccaac atagatgatt ttttcttttt
    10561 tctccatttc tttcagtatg aataaaggaa aaatcaacgc aaccaatcct aataacccca
    10621 tagtgtatgt tgtaatatcg ttatttaacg cttgaatgat actatctgaa atagaaagtg
    10681 cttctagctg caaatctgtt ttgaagggac tgtgtatttc ttgataattc atctgctgca
    10741 acataggata ccaaaaatag ctagttaatg cacaagtcaa aaaaacagcc tttactaaat
    10801 tcacgaccac aaattttata tcgtttttta tttgcttgct taaaaacaaa cgaactataa
    10861 tcatacaagc aatcaatgca ctacacaata gtgtagttaa gacatgagta tacacaagta
    10921 gacttatact gacagataaa gcgatgactt ttttttctct attgaatatc aatccataag
    10981 cataatataa aacagctggg ataatcgtaa gagcgattgc ttctgcaatt gcggatcgat
    11041 aatagatatc aactgttcga taagttgcaa aagtatataa acaactaaat aaaaaagcag
    11101 catctatctt tttaaaaaac ttttctccat aataaaaact aatcagaatc gtacaaatat
    11161 ttaataacca aatatataga acataactta atattaagtt atttgaaatt ccataaaaaa
    11221 cgactgcagg aattattgta agaaaaggat aaaaatagtt cagtccatct ccataattgt
    11281 taaatgttgt atagttgatt ggcccttcaa aaatagaaga taaccctttg attcgtgaaa
    11341 gatgaaaaga taaatcacct gttgtataaa tttcttgagc atttctcata aaatttactg
    11401 aatataaagt actaagtaag ataagaacta ctaaaagaaa tgctttttta cttattttca
    11461 tagatttgtt gttctccttt tttaattcta ttttcagtat actagaattg tcttttttta
    11521 ataagttttg cgttaaattt ttcatagcac tagtatgata tactagaaaa gaatcaattt
    11581 ttattacttt taaataggag aaaaaaatga aaaaaaacaa aaaccaaata atagatatca
    11641 tttttatgtt atttctaagt tttgcttacg tcatccctct attcatgtct aagggaatct
    11701 atcatagtgt caatcaggat acttactttc atttatctcg aatcatagga ctagataatg
    11761 tatggagttc tccagtaaac ttcaataact tcgatcatca tggaacaatg atgaatattt
    11821 tttatccatg gcttactctt tatccagctt ttttatttta taaaatgatg ggaaatcttg
    11881 ttttaagtta taatatctac tattttttta tcactttttt aacaatggtc gtttcttatt
    11941 tttctatgaa acaaataaaa aacaatcgtt atatttcgct gttattttcg attatttata
    12001 cattttccgc ctatcgtgcc attgacatat tccatcgtgc ttcattggga gaagctgtcg
    12061 ctttaacatt tttgcctttg atactaatgg ggtgttacga aatttatatt agagattacc
    12121 aaaaatggta ctggttgtct attggcatga cgctagtagt gtatactcat ttactttctg
    12181 tcgctatggt ttctgtattt attggaggaa cgttgtttct ttctttttat ttttgggacc
    12241 aaaaaatcgc tagactctta agtcttttaa aagctaccgt cctcaccttt tttttaagtg
    12301 cgggattctt aattcctttt attcaacaat ctagagcaca agaattgaaa gtcccactgg
    12361 gaaaagaatt aagtggtatg gctcctagtg atatgctgac acatatttta aataataact
    12421 ataacaacta tactattgga ttattcttat ttctcggatt gattggtgct tttattttta
    12481 taaaaaaatt aactgctgat gatttattta ttttctttct tggtgtattt gttctttttt
    12541 gttcaaccaa tttatttcct tggcaacttt ttaaccatac accagttaag agtctacaat
    12601 ttgtctggcg gttaaatggt ttttcttctt tatttatagc ctatacgatg agtatagtga
    12661 tctattatat tttttctact aaaaataatt catggaaaat aatggtcttt acgtttcttg
    12721 cactaatatt gcatctctca ggagtaagca attctatcca tgctaacaaa gattcattaa
    12781 cacttatagc acctgaagac gctgttgcta tagctgaaaa ttacaatcat actgattatg
    12841 caaacaaaga gtcaatctat catcctgata tgattaataa tgatcaatat ttactaggta
    12901 accaggaaat aaatccaaca actcatttta tgagtaacaa acttacaatt gaattagata
    12961 attcgaataa taaaaatact gtcttgacga cacctattta tcgttataaa gggcaagtgg
    13021 cttctattaa tggcaaacta gtacaaacaa aattatctaa gtttggcaca actgaattaa
    13081 ctattcctcc aggtataaat aaagtcgtta ttacttatca gtacacaaaa ctagcaattg
    13141 cttcaagata tttatctatc gttactttga ttttattttt actttatcga tttacttttt
    13201 ctaaacaaaa acaaccgaga agagaagtac aacattctca ctagattttt atttaagaaa
    13261 gactttttag ctgtacacat aaacaggcta aaaagtcttt ttttatattt ctttaatttg
    13321 cttcatacat tatttaacat cgatatttat actgtgaaac atgcgtttca tccattatca
    13381 aagtattttt atcgataata aacttcatcc aaaaacagac cttgtgcagg gacggtttct
    13441 cctgcatgtt gccgcacttt gctttcaaat atttcatcga ttgtttctat cggcatggca
    13501 cctgtgccga tttctagtaa tgtccccatg ataatccgca ccattttata taagaatccg
    13561 tctccgacaa aagtaaaatg aagcatttct ccctttcgtt cgatagataa ttcatcaatc
    13621 gtacgcactg tggatttttt tgattttttc aatgcagaaa aaccaatgaa atcatgtgta
    13681 cccactaatt tttcgcaagc tgcattcagc ttgtccatat ccaactcttg agggaaataa
    13741 aagctgtggt ttcgctcaaa tgctgaagga atcacgttgt tccaaacata atagctgtat
    13801 tttttgccca ccgcattata tcttgaatgg aagcgctcag gcatttcttc gattcttttg
    13861 atgacaatat cccgcggaag gtaacgattc ataaagtcga tcatttctgc cagtccaatt
    13921 tctgtgtttg ttttaaaatt tgctacttgt cctcgcgcat gggtgccagc atctgttctt
    13981 cctgatccaa taatttctat ctcttcattt gtcatctgat gcaatacttt ttcgattttt
    14041 ccttgaatgg ttttatcgtc atctccaagt ctttgccatc ctgaatatct ttttccgtca
    14101 tattcgattg tcaacttgat gtttctcata ttttagtggc tcctctgtct tccatttggt
    14161 cttctctcta cctttttaag attgtagcac aaaatatagc tccttgcttc tctaagacag
    14221 aagctataca agaaaactgt ggaaatatat atttcagaaa aacaaaaaac agccaccgga
    14281 tgaagtatcc gctgactgtt caatttgacg gctcaagttt tactgtttga gatgcaagat
    14341 actcaaccag gcaatgggca cataattaaa gaaaaaactg attctttggc aaaaacctat
    14401 tttattcagt aatggccaag aatttataga aggaattctt gccagtccat aaatcgccgc
    14461 aaaaagcaaa ctgatcacag acagtccaag gtataagtgg cttacgtcca tttgcccatt
    14521 ttttcgatag atcaagaaaa gaagaactgg aaacaataaa aaaccactgt aacctaaacc
    14581 tgatccgata ttatggaccc atgtcgagac attccacgaa gattcatggg tattgatact
    14641 gaacaaacct gtaaagatac aatcacctat accatagaac gcgatggctg ctgtaactag
    14701 ggtagctaac acacgagatg tactaacaaa tgcagcatac aatgcaggca atgacaggat
    14761 gaaaaagatc cctgaaacca cagaccaaat aagaaacgct tttcttacag gactggaaac
    14821 ctctccaaat acactgatca cgctcttcat ttgatctaat tcaggataat acaatcctaa
    14881 tacgtacgga gtcaaaaaat cactcagcac gcctaataat aaaaagaaaa aaccaaattt
    14941 acgtaaaaaa tccatgatac gcctccaata acaattacct atgcttttaa ttgttattat
    15001 aaacgacttt atcttgattt aacagaaacc tctatttaca cgaacatcta tataactcat
    15061 taaaaactcg aatcatttta aaaatactaa atatctaacg ttcaacgttc acttaatcca
    15121 tctaaacaat ctttcaattt gattttttcc ccacttcttt tacgcgcgcc tagctctcct
    15181 attgcagtaa acaacagatc acttgacgaa tttaaggcag tttccattga atcctggata
    15241 acaccgataa taaatccaat accgacgatt tgcatcgcga catcattaga gataccaaac
    15301 aaactacatg ctaatggaat caataacagt gaaccgcccg caacacctga agctccacaa
    15361 gcagctaaag cagacaaaat acataaaagg actttcaaaa caaaaggtgc ttcaatccct
    15421 aaagaatgta cagcagtcaa cgtcatcaag gaaatggtaa tcgcggcacc gcccatattg
    15481 atcgtagcac ctaacgggat cgatatggaa taagactctt ttgtcaattt caatctttca
    15541 gccaacatca tgtttatcgg gatatttgcg gcagaacttc gggtaaagaa agcgggaagt
    15601 gcgctttctt tcaaacaaaa gaaaatcaat ggatagggat tttctttaat aaacaaaaac
    15661 gtgaggaatg ggtacacaac taatgcgaca aacagcatcg taccgatcaa aacaagtaaa
    15721 agctgcagat actctgccaa tccagcgatt ccagtcgtag caattgattg ataaaccaat
    15781 cctaaaatcc ctatcggagc aaatgcaata atcacttgta cgacttgtga caacgctata
    15841 gatatctgat ccaccaactc ttttgtcgat tctgaacgtg ctcgaaaagc tagtcccaac
    15901 aatacagccc agaataagac gccaaggtaa ttcgcatcaa taagagattg aacaggattt
    15961 tgtgtgacat tcgtcaacat agtagataaa acactttcca aactttgcgg tgctttttca
    16021 gatactgttt cttgtaaagt aataggaaca gtaaaaattg aacttacaat aactgctaca
    16081 agtgcagaaa gcaatgttcc cactaaatat aagatcaaaa taggcttcac gaatgtctta
    16141 ttcccaattt tatgcttggc aattgacgcc atcgtcaaga aaaaaaccag aataggtgcg
    16201 attgccttca agcaactgac aaacaacgtt cccaatattg aaataaatgt ccatgtgggg
    16261 aaaaagatac caaacaatat tccgactaaa ataccaacaa caatacgttg aactaacgac
    16321 atatccaaaa acttctctaa caaatgacca ccctttcaca attttaacaa tcattgtatc
    16381 attttatgca aagaaactaa aggtataatt ttctaaaaat tctaacaata caaacaacca
    16441 tttcttgcta attttgtact ttttctgtct acacgttgtt ttttgagata ttctaagtta
    16501 ggatgttatt aatgggcaac ttatcaggaa acgttttctt atgtttttgt ccaatttttt
    16561 tgtgaggtgt ttattcttga aaaaaataaa gaaggagagt tatggcttcc tgacaatctt
    16621 tgtcgcttgt ataatgatgg ttttacagcg tatattgatt tcagcagtca ctattcctac
    16681 atatgcaaca aaccctggtc gattctacat ctatacattt ttacaaactt ccgttgttat
    16741 tggtgcaaat ttgatcccac tttacatggg ttaccaatat caaaaaatcg cccgtttaaa
    16801 agttttacat tatttaagtc gttttgcctt tatattccta atagccacac tagtttgcaa
    16861 tatctttttt tacgtacaag ctggcagctt gaatattcgt gattattggc ttatcctaac
    16921 acctatcagt cagaattact ttacttatgc tgtttcttgt actttgcttt tttgtagtat
    16981 tccttataca gttggatttt taaataaatt atcgaaaaca acaattaaat atctactatt
    17041 atttttaaca attggattag ttggctgctc tactctattt aataaagacc cgtttgcatt
    17101 acagaatgga cagagtattt tatggatttt ttatttattt ttaatgggtt atggtttgaa
    17161 agaatggaac tggaaagaaa aggtccgcta taagcttcct caagtattta tcagcttgat
    17221 tgttttattc ggtttgatta ttttgatgac acaaatttca ctcatcatca gaggaaatac
    17281 agatacagcg ctgaggtttt ctaaaccata ttctctcttc gcaatgtatt atactttctc
    17341 ttcgttttta tttttagaaa tgctatctga taaattaggt ataaaaggaa gaggaaattt
    17401 tcttgctgta tttttgattg tcactcaagt tatatgtaat ttccctttag tttcttatcg
    17461 cgtatctaca tttatgaaac gtgaatttcc aaactcaggg aaggcatggc tgataaatat
    17521 cgggcagttt tttatttgat atttattagc agtgtccctc ctagttatct ttttatttta
    17581 tatacaaaaa atccgtttca ttcatcgttt catcgaatac ttcgcttttg attcgattga
    17641 acaattaact cagcgttgga aaaaaagaat tcattggctt tttgttcata aaagttattt
    17701 tttagttgca gcattttttt actgctttac cttcgtgcag attttcgttt tagaaccaaa
    17761 agaaggttgg aaagaaacta tccaggttgc cttaaaaata tttactcagc gacaagcacc
    17821 catgatcctg acaatcatca ttattatggg atttttcttc ttactattgt tgatttccaa
    17881 tcgtttttgg tacgccttga ctgctacctt gatcattaac ttactgctca ctatttcgac
    17941 agtaatcaaa atggaaatgc gggaagagcc tgtctttcct tcagatttaa aaatgctgac
    18001 cggattgtca gaactattat cgatggttag cccagtctta ttaatcgtgg gcggattgat
    18061 ccttctccta ttattgatta ctagtattat tgtacaacgt agactacaaa aacagtattc
    18121 cttgaagatt cattggaaac gtcgatttat cagtatcgct gtattattag gatgtttttc
    18181 gggtgtcttt ttcattaatc acaaaaattc gccttctttc ttgctgttta atttatttaa
    18241 agtaaacaag actttcttta accagcaagg cgccgtaaaa gataacggac cagtcattca
    18301 attcctaaac aatatcgaca ttaaaatcat ggaagaacct gctggctatt cgaaagaaaa
    18361 aattaatgga ataatgaaaa agtacaacac aaaggcagat gaaatcaacc aaacaagaag
    18421 tgactggtta aacaatcaga cgatcgttct caatttaagt gaaagcttca gtgatcctgc
    18481 acgtgtccct aatctgacag tacctacaga ccctattcct tacatcaaaa gcattataac
    18541 agaaggaaca ggaggattga tgttatccgt cggttatggt ggcggaacag ctaacatgga
    18601 atgggaagga ctgacaggct tgagtatcag taacctgtct gcttcgttag ttacacctta
    18661 tacccagcta gtggagcgtc agaaaataag tccaaatatt accgatctat tcgatgaaaa
    18721 aatagccatc catcctttta cagctgctct ttatcgcaga aaacaagtgt ttgataaatt
    18781 tggttttgac aggttctatt atttagacag tccggataaa ttaacttata cggataaaat
    18841 agaacaaagc ccacgcatat cggatgattc agcatacaaa gaaaccttga aagctttaca
    18901 aagcaatacg gaaacgtcac aattcatcca attatccact atgcaaaacc atatgcctta
    18961 tgatgattac tatagtgaat tgaactacac tgctgaagga actgctgtca ttcctaatag
    19021 aagaaaagaa ttacaaactt atatgcaagg ccttcactat accgatacag cagtaaaaaa
    19081 attcatccaa gaaatcgaca aaatcgataa accgattacc tttgtttttt atggtgacca
    19141 tttacccgca atctatagtg ggaatgattc gaaaaaatat ggattagaac agcaccagac
    19201 agattatttc atttataaca acaagtatag tcgtgaacaa gcagctacgt taaataaaga
    19261 agttgttgct cctaataact ttggtgctat ggctcttgag catgcgaata tcaaagtaac
    19321 tccttactat gcgttgttga cagaagtatc taatcatcta cctgcattaa caattgatcc
    19381 tacagaaagc ttgtctaatc gttttaatgg aaaacaggtc tttgttacag aggatagtca
    19441 aattatccaa gaaaaaaatt taactgagaa acaaaaagaa attctagaag attatcgtct
    19501 gatacaatat gatcttgttg caggtaacca gtacagtgca acatgggcga cacaaaaaat
    19561 aaaagactaa caaataaact gagcaagggg ttgtgataaa agacatgtca caaccccttg
    19621 tttatctcat taatttgctt gtccataaac gatttgatta aagccatttt tcaattcatt
    19681 attcgctgtc tcgttgaagt aaagataatt atcgggattc ccctgatttt gttgtgtcac
    19741 caatactttt ggctgatttt gctgaaaccc gaatagatag acgtgctttt tcagatatgg
    19801 ttctgtctct gcatcggaat agacggctgt gatttgatag tctgcttgac ctgtcccact
    19861 ttctgaccat tctgcagaaa caggcactcc accaatagcc atcttccact ccccgttcaa
    19921 aatagcttga gggactttaa gaccatataa gtccacactc atttgattcg tataactttt
    19981 gtattgttgt cccattgttt ggccccattt ggtcataaat gcctctaatt gtgcggattt
    20041 gtctgcattc cacagtgttt gttcaacttg ttcgctgctt tctgaagagg aagatgaagc
    20101 ctctacagaa gaacttgaag tagatgtttg agttgtttca gtcgttttgg aagactctga
    20161 cgaagtagag gtatctgctg tttctgtact tgctgcagtt gacttgctct ctgtgctagt
    20221 tgttgtagta gttgcaactg ttgctttcgc tgtttctttt gttgattctt ttttcgattc
    20281 attcccgttg gtacaaccag ctaacaaaaa agctagacca attgttacta aaatcctttt
    20341 tttcaattaa aaaactcctt tcgattactc ccacttcttt attctatcaa ataagcaatg
    20401 tgattttatt gatttttcgt attttctcat caatctttca aatcttctaa aaactgagaa
    20461 gcacccgctg atgttgtctg taatacatca gcgggtgctt cttttatttt aatcttcgtg
    20521 attttccaat ttacctcacg ctttatattt gtatttgaag aaacatagac ttttagtcat
    20581 catcgtcttc ctcatccaaa tcttgtcgga cagtttttcc ttggagtctt tgtacttggt
    20641 agatgatttc tttattgaaa cttaaaagtt caacaagcaa ttgttgcaag ccatattttt
    20701 cttctttttc agctaatttg attgcccgtg tgataaagac attttggaaa tcaaaatctt
    20761 ttgcaaagtt ttccaacatt gttgaggctg gtaaatattt gttttgacca tgttcgtaaa
    20821 tttttccata acgcatgact tcttctaaag tagacggaac gatttcattt tcaatcacta
    20881 actcatgaga gattttattg aaccatctcg cttcttgatc agcatattct gaaaaaaggg
    20941 tacttaattc ttgcgaagat gtacccaaaa catacaattg tgcttggatc aatttcattt
    21001 cttctaattt aaaatttgct aatacatgcc ccaccatggc gccagcagtt ggtttatgat
    21061 ggtcgatatc tgcttgtttt gtttcttgaa caaatttttc ttccactata ttcattgatt
    21121 tacgcctctt ttacttttgc tgattggact tcataaccta atttttccac aactgcagct
    21181 aattgttcag ggtggacttc atctggatca aagtttgctt tgattttgct cgcattaaac
    21241 aaaactttcg tattgcttac cccaggctgt tgagaaaccg catgttcgat tttttgcata
    21301 cagcttggac aagctagtgt atctaattgc ataataattt ttttcatcgt agttcttcct
    21361 tcctttttct tgatagtctt attctagatt acagcttcca taaaaaaatt gattcttatc
    21421 aagttttacg atttttcttc tttgctttcg tgtccaaaat acaataaacg catcgcgtta
    21481 aagataacga tcaagatact agcttcgtga acgaacatcc cactagccat gtaaataaac
    21541 ccggcaaata agccgttgag taaaaataag acgacaccaa tggcaatcac tacattttca
    21601 cgggtattta aaacagtttt cttagccaag cggtaggcat gaaccaacga ttcaaaatta
    21661 gattgcatca agacaacgtc actcgtttcg atagcaacat ccgttcctga tcccatagca
    21721 atcccgatat cagccgcagc caaagaggga gaatcattga ttccatctcc aataaaagca
    21781 acgcgcaatc cttgttcttg gtattttttc acaaattgaa ccttttcgtc tggtaataat
    21841 tcagcatgga cttcatcaat gcctaacata tcagctacat aatcagcagt catctgatta
    21901 tcacctgtca gcatcactaa ctgttttgct cctgcttgtt tcaacttagc taattgttca
    21961 gcgacttctg gtcgaatcac atctgacaca cctatgattt gagtgatttt tttgtcgatt
    22021 gctacaataa tgaccgagct gcctgtttgt tgtaattgat gtaaatcttg tacttgcttt
    22081 ggtgtgagag aaagtttgtg agcggcaatc aatttggtgt tgcccgcaca aactgtatga
    22141 ccatcaatct cagctgtgat tccttgtccc ttgattgtct gattatcttt gactgctaat
    22201 tgttgaaaat caatgtcttt ctcctgagca taatggatca cggcgcgccc cagaggatga
    22261 tcggataacg tttctaatcg agccactaaa gctaagctgt ccccttcatc ctctgtatag
    22321 ttcttgatag tggatacagc agtagttcct ttggtcaatg taccagtttt atcaaagaca
    22381 aaagtatcga ttttggaaaa agtatccatt gcttcaccgc ctttgactaa gatgccccgt
    22441 ttcgctccat taccgattcc tgctacattc gaaacaggcg caccaatgac taaagcgcca
    22501 ggacaaccaa gaaccaaaac agtgattgct aaacgaaaat cacgactaat cagaccaaca
    22561 accaacgcaa taagtaaaac ggttggagta taatactgag aaaatcgatc aatgaatttt
    22621 tccgcatggg atttggtatc ttgtgcttct tctactaatt cgataatttt ggcaaaggtc
    22681 gtatcatctc cgaccttagt tgcttcaatc gtcacgtagc catctgctaa aatcgttcca
    22741 gagaagactt catctccact gattttgacg acttgtcgtg attcaccagt cactgcggct
    22801 tcatccgcat accctttccc gtcaatgact cgtccgtcca caggaatagc tccaccagtt
    22861 tttactaaga cgatatcgcc ttcttccacg tcatcgattt ccacttcttc agtcgttcca
    22921 tcttcttgga caacactagc agtagatggg gccatctcgg tcaaagcctt gattgactcc
    22981 cgcgttttgg tcaatgtttt ttgttccaga tagctaccaa acaaaaacaa gaatgtaacg
    23041 atagctgact ccttgtattc tccgatcata aaggcaccaa gcacagcaat agtaacgagt
    23101 aaatcaattg agataacttt gactttcact gcctgatacg catggatcat aataggtatg
    23161 accgcaataa tagaagctat ggctagagca atgttataga acaagacaaa accagtaaat
    23221 tttccgataa aaccaaaaat aatcaacaca gctgataagg cggtgatatg atttcgatgc
    23281 tcttggatcc acttttgtaa tttcatcttt ctccctcttt tcctttcatt cttacgagac
    23341 taagtataga tacttatcca tcataaaaaa ttgaccacta tcaagtttag tcaaaaaggc
    23401 ggttttcttt tggtagaata cactacaaaa aatagttagc aagaagactc actaaaagtg
    23461 atcttgccaa ctattgtcgt ttctatcgaa attttcattg aatgattttc ctgtacagca
    23521 tacatcggag atctatttct ttatctaaga tttttatctt gctaattttt tcctttagtc
    23581 tttcaaatcc acgtcgttca tagaattgat acgtacagcc actatctgta aaaaggaata
    23641 tttcttttcc tttctctctt ttttcaaatt ctgttagcaa cttggtaccg atgccttttg
    23701 ttttcaaatc aggatttgca gctaaaaaaa tgatctctcc atctggcgaa tgcttcttta
    23761 aatatgctgc aagcatttgt tcatttgctg tctcgtaagc tcctttactt tctttagcaa
    23821 atatattttg taaaaaatcg aatatcttaa catatatttg ctgtccaaaa gaacgatgtt
    23881 tctttttttc tccttttatc tccgccagca aaacacctgc caactcgtcc tctgcataca
    23941 aagcaagaat ttgagttgct cgattcaact ccatatacaa aaaatatctt ccgtaaagat
    24001 tcagcagaag ttcattttcc atatatctct caaagttcat tccttcaaca gcaaaataga
    24061 tagctttttt gtaatctttc ttttctaagg gtttaaaagt tagtttcatc atttcgcctc
    24121 ctcaacttca atgattgtgt tatacttaca ttataataga cacagtgtct actatctata
    24181 atcaatcatc tataagtttg tcgattaagc gacagctttg ctttgatatg tcggataagg
    24241 agataggtaa atgaaaaaga cagatagacg cgtcaaaaaa acagaaaaag ctttggctga
    24301 aacactatcc acattattag taaataaaaa gattcaagca atcactatac gcgaactaac
    24361 agaaacggca gatgtacatc gttcgacttt ttatacacat tataaggata tctatgatct
    24421 atatgatcaa ttagaaagca acttttttcg ggatctaaat gaaattctct cttatgatcc
    24481 agcccatagc tatgaggaac tatacactcg cttgattgac tatgtttacg caaatacttt
    24541 tttatatcgc ttatttatcc tcaaccatgg tgatatttcg tttcagaaga aagttaaaca
    24601 agttattgaa gaaaactatc tgaacatatg gctatatgaa gacaagaaaa cgattatcac
    24661 agaagaaatg agattcttta ctacgtacca cgtccaaggt tgtctatccg ttctcaatcg
    24721 gtgggtagaa agcaattaca agtactccaa aaaagaatta ttagaactct tacagaaact
    24781 gaatgatcac atagagaaaa tcatgccttg aataaaaaca tactaagctt agttaagaag
    24841 aaccgtaacc ttctcgtata acaactgaat cttatttagc caaattgttt actgaagatt
    24901 ttttgagtaa aatttctata attcctactt attgaaaagt agtttgtagt gctgcttctc
    24961 cctctctaag tgatatatct tgttgtacca atcaaattca cctcatcatt aataaattac
    25021 ttttaggcta atttattaca acttataggt tgaagctgta accaatttat agtcatatta
    25081 tatttttatc atccagttta ttgaattctt ctcgtttacc tcaattcatt ttcaagggga
    25141 atcagctcat catctaacat tttacgccat gctcgcttca tttcttgtgg gtatttaaat
    25201 ggtttcgctt ctggatgttt ggctttatat ccttcgtaat ccttactgtt gataattgct
    25261 ttcatattag gaattggatc ttcacctggc atatattgca gattagaagc atgtaatttt
    25321 ttctcagaaa caaaccactc attggaaaag acatgaatca aatcatcact tacttgtgta
    25381 aaatagcgac gtttaatgac aaagatatct tcattgctat ctagtgtacc ttcatttctt
    25441 tgtgcttaaa ttcaaagata attttctcaa taaagatttt tgattccagt aagattcctt
    25501 tactcctttt acaacatagc gttgtccttt gttttgaaac atcttcttcc cctctttgcc
    25561 attaaaaata gcccaattct ctataaagaa gattaagctt cattattata atataaagtt
    25621 taaatttggt ttattactta ctttttctta ttctctagta agctaatatt gaaaaaaatg
    25681 taatcagatc attattttta tatgttgtaa cttcctgata agaaaactaa ttaaaagaaa
    25741 gaggtaacta cataaactaa ttaaaaaagc taacttcata gttggaggat aaatggaaga
    25801 aacttcaatg attgtttctc cttgtgttcc ttcaatcgac actagtccta ttatcaatct
    25861 ctcttttttg tccatttact tttgcgactg ttccccaata atcaaataca ggaagcgtta
    25921 ttttttgtcc tttagattca tgtactgtat agactatttt cgaatccatc acttgataag
    25981 gcatagtatg ccattccttg ccaattctta cttcatgatt ctgcaaatgt tctttgtatt
    26041 gtaatgcttc cgtgggtgca tagtcataaa gtcctccggg aaggagtgtc gtgtaacgaa
    26101 taagattttc ttgattcatt accaagtgat ctctctgagt gacaacttta tgttcatagt
    26161 ttgcttttgt tgcagcagat acagccaaca gtaataaaac aattgccgtt atattaacta
    26221 cttttctttg cttagctgat gtgctcttca tgaaaacgaa cgataaaatc aacgagccaa
    26281 acagtacttg aaagcctaag attcgccaag gaaattgaat catttgaatg ggtgtatttt
    26341 gtaataaaga ccatggaaaa aatttggtgg ataagagcca agagactacc gcactaaggt
    26401 aaatcacttt ttctttgcga ttaaatcctt taaaaaagaa gagtggacag atcaaagaaa
    26461 taataccaat gattcctacc gtaaaggttg tcaggtcatt attcatcgca ccaatcaaac
    26521 tatccgagac atttaatgct tggtgttgta aggtcgtttt atctggctca ttaatcgatt
    26581 gaaccatgat ttgtcggaac atcggatacc aaaagtatgc tgttaaaaga atcgtcattc
    26641 ctactgcctt tgacaactcc acaatcaacc ggatgctttc tttttttctt gtttcactta
    26701 ctacaaagcc gcctaagact agtaagccaa cggctattac actcataaaa gtggaaagaa
    26761 catgtgtata aatgagtaag ctcatactga ctgctaatgg aattgcacat ggctttttct
    26821 cataaatcag tgcgtaagca taatacataa ctatcggtat aaccgtaagt gcaatcgctt
    26881 cagcaatagc agaacgatga taaatatcaa tcgtacggta cccataaaat gtatataaac
    26941 agctaaatat aaaagccgca tcgattcttt ttaaaaattt tagtccataa tgaaacatca
    27001 gcatgatagt acaaacattc aatagccaaa catataagat ataactaacg attaaattat
    27061 tacttatcca ataaaaaatg actgccggaa aaaaggtcaa gtagggataa aaataattca
    27121 ctccgctccc ataatgatta aacgtcgtaa aattgattgg accagaaaaa attgaagata
    27181 accctttgac tctagcaaaa tggaaggcaa tatcccccga tgtagaaata gtatgaacat
    27241 ttctaaaata attgattgaa taaagtccag caataattgc taggagaatg gacaatatta
    27301 tttttttctt tacttgcatt acttaattat atactccttt catggattgc tttatctagt
    27361 ataacttaaa ttttaaataa atcgttattt atttttactc tcacttaaaa aagcactaaa
    27421 attataagag agggaggtaa tggtctgaac accaagaatt gttccaacat cgaattgctt
    27481 aactaatgag ttcaaagctt aattatgtgg ctcttatatt taccgagcgt ggattgcgat
    27541 ctctttagcg aaacaaacat ctttcataaa aaaaacagcc atatccgctt tcttggatat
    27601 gactatttac tactacttta ctatctttca ttcttcttgt ctcacttatg ataaggcgcc
    27661 cccgcattga tccggaaggc acggtagatt tgctcgacaa ggatcagacg catcaattga
    27721 tgtggatacg tcatttttcc aaaagagatc tgagcattgc ttcttttcat gacggcttcg
    27781 ctgagtccta aagagccgcc gataacaaaa acaaggtggc tttttccttg gatacctaat
    27841 tggtcgattt gtttagcaaa agattcactt gtaggatttt tcccttcaat tgctaaggca
    27901 tagacgtatt cttgttcttt gattttcgct aagatccgtt ccccttcttt ttcttttact
    27961 tgtctcattt gcgcttcgct taaattctcc ggggcttttt cgtcaggaac ttcgatcaat
    28021 tcgatttttg cataggcatt cagccgtttg acatactcat tgataccttg gatcaggtat
    28081 ttttctttta attttcctac tgagataatt ttgatattca tgttttctct cctttggtct
    28141 tcatgctcac tttgtttatt ataatcaatt ttttcgtcta tatgtttatt ttggctattt
    28201 ttgttaaaaa gatttcccca acagttatgc acaacatcca caggtttatc cacagaaaac
    28261 agtgttattt taccttattt catcacattc attaggcaaa catacgtttt ataagaattt
    28321 atccacttta tacacaggct gtggatatgt tttatccctt atttttccaa tcctatccgc
    28381 gtagttttac acagtatctg tggaaaaaga aaatcaaata ttttcatctg aaaacaatga
    28441 tttttcctaa agtttttcat aaaaagttca cagtctcaaa gtagactaaa gtcaacaaat
    28501 aagactatac taacagtaga taaatgaatg taattggagg aggaacacat ggatagaaaa
    28561 aatgtgacac caaagatgaa aaaaagtaaa aataatagcc tctggcgtaa gttaggtctg
    28621 gggcttgttg gagggatcgt tggaggacta ctgacagctg gcatttttta tgcagcgatg
    28681 ggcactggca acaatagttc tactgctaca agcggcaatc agaattctgc cggtgaaaca
    28741 gttgtggaaa atgtcaaagt caatgtagat tctgatatca ccaaagcggt agataaagtt
    28801 caagacgctg tcgtttctgt tatcaactta caaagtcaaa accaatcaag cggttttggc
    28861 ggactattcg ggcagcaaga agaaagtagc agctcagatg atagtaattt ggaagcttat
    28921 agtgaaggaa gcggcgtgat ctacaaaaaa gacgggaata ctgcttatgt agtcacgaat
    28981 aatcacgtag tagatggcca gcaaggtctt gaagtcttga tgaaagacgg tacgaaggta
    29041 aaagctgaat tagttggaac agatgcttat tcagatttag cggtgttgaa gatcaattca
    29101 gacaaagtcg aaacagttgc ctcttttggt gactcaagtg ctctgaaggt tggtgagcct
    29161 gcgattgcga ttggttcccc actaggatct gaatatgcaa attctgtcac ttcaggaatc
    29221 atttcttcct tgaaccgaca agtgacaagt acgaatgaat cgaacgaaac agtcaatatc
    29281 aatgcgatcc aaacagatgc agcaatcaac cctggaaatt ctggtggtcc tttagttaat
    29341 atcgaaggac aagttatcgg tatcaactca agtaagatcg caagtacttc tgaatctact
    29401 tctaatgtaa gcgtagaagg aatgggattt gctattccaa gtaacgatgt agtgaatatc
    29461 atcaatcaac tggaaaaaga cggaaaagtg acacgtccag ctctaggtat cacaatggtc
    29521 gacttatctg ccgtttctac tcagcaacaa gaacaaatct tgaagattcc ttcatctgta
    29581 actaacggtg tcatcgtgac aaatgtccaa acagctacac cggctgaaaa agctggattg
    29641 aaacaatacg acgtcattac taagatcgat gacaaagaag tttcttctgg tgtagaactt
    29701 caatctgctt tgtataagaa aaaagtggga gattctgtca aaatcactta ctatgaaggc
    29761 tcgaaagaaa aaacaactac tgttgaattg acgatcgatc aatcggcatt aaaacaaaat
    29821 caaaatagtg aaaattaata aaaaagaggt tgtgacaaag acttgtcaca acctctttat
    29881 tttggaaatg cttctccgac tacggcatcc tcaggcaata ctaagattcc tttttttgct
    29941 ggagcatttg gcaaatgcaa ttcacgagcg gaacagatca ttccatagct atctacccct
    30001 cgcaaagttc ctggccagat catcattcca tctggcatca tagctcctgg ttttgcaacg
    30061 acaacttttt gtccagcttc gatattgggt gcaccgcaaa cgatttgcaa tactgtacca
    30121 ttgtctactt ctgtctcagt aatcgataaa tggtcagaat caggatgtgg gacgcatgtt
    30181 tttacgtacc cgacaacgat cttcggttca tgatcagctt caagctgttc ttcgaatcct
    30241 gcttctttga tcaatcgatt caatttcgct aattcctcat ctgttaagct tacttggccg
    30301 ttcccttcga ttgttagatg atcggagata tggaaaaagt tccaagcaac aacttgacct
    30361 tgttcatctt tgatccgagc aacattccat ttgcgttcaa cagtgttttc tgttccttta
    30421 tcgtcggcta taataaccat caaggtgtct ccgacgtgtg ctttgttata agcaaaaatc
    30481 atgttctttc ctccattatc ctttgctgag atttatctta acaaaaaatt gcattcactg
    30541 catgaaattt ttttcatgct ttatttccga agaagcctct tctggaacac catttattcg
    30601 taacaaggga ctgtgacaag acttttgtca cagtcccttt actgttttag ttaattgatt
    30661 aaaaaggtcg tacaagttcc acttgcttaa ttacttggct gttcttcttc gtcagaaacg
    30721 atcaagacat cacaaggtga atgacgtatc acgtagctgg ctacacttcc tgtcataaag
    30781 cgttcgactg catttagacc tgactgccca accatgatta gatcgatctc atattttttt
    30841 ggcaatttga ctgccatgga ttcacgaaca gaaccatatg caataattcc ttctatattt
    30901 gtgtaatcaa cagatttccc atattcttta cattcttcga tcatttcctt ggctgtttca
    30961 gtttgctgat cgactagatt ttgatcaata ggtgcatagc ccataaagtt ataaaattgc
    31021 tgatcgatta cgtttgctac atatactttc ccattattac ggcgagccac ttctaccgct
    31081 tttttgaaag cagcaagtgc ttgttcacta ccgtcgatac ctactaaaat attcttatat
    31141 tcttgattca tcttgacccc tcctaacaga tgtcagcctt ttgttacact ttctaggaac
    31201 gattcgattt cctgttttgt tttacgatct ttattcacta gacgacctaa ttcttttccg
    31261 tcttggatca cgacaaaact tgggatacca aaaatgttcc attcagcggc tacatctatg
    31321 tacttgtcac ggtctacttc aataaattgc caatcagaaa agtcattttc aatttcaggc
    31381 atttgcggtt tgataaaacg gcagtctccg caccagccgg cagtaaagaa aaatacactc
    31441 tttccagttt ctacgtagcc tgctaattct tctaaactat ttgggataat cattctttgt
    31501 cctctacttt ctgtttatgt atttttcaat taaattatac acctttcttt cgaactaaca
    31561 aacattgatc ctctttttat aaaaaaaatc tggcaaaaag caatcttttg ccagatttta
    31621 tagtttcact atcgcattat tttccgtata cgatcgtatt tactgttgat ttatccaaac
    31681 ctttcagtgt ttgaatcaac atttctcgtg cagcttcaaa atcgcgaata ctgaacatcg
    31741 tttgatgcgt atggatataa cgtcccacta cgccaatcac tgtacttggg attccttcgt
    31801 tttgtgtatg agcagcacct gcatctgtac cgccttttga aacaaagtat tgataaggga
    31861 tattgtttgt ttcagcgata tccagcaagt actcgcgcaa acgaggaagc atgatcaaac
    31921 ctggatcttg gatacgcatc aatgtacctt ctcctaaatg tccgaatgtg ccatttttcg
    31981 tcactgtatc atctgctgca gagcagtcta ctgcaaagaa cagatctggc ttgaatttat
    32041 tgacagaagc tttagaacca cgcaagccta cttcttcctg gacattcgcg cctgcaatca
    32101 atgtatgacc taatgtttca ttttgcaacg cttctaaagc ttctaatacc attgtacagc
    32161 catagcggtt gtcccatgat ttactgataa tattctttcc gttggctgtt ttgatcgttt
    32221 cagcgtaagg aacgattgtg tctcctggaa ggacaccgta actcattgcc tcttctcttg
    32281 attcaaaccc agcatcgaac aaaatatcgg ttacttctac tgatttttgt ccgcttgttc
    32341 ctcgcaataa atgtggggga acggaagaag aaatacatgg ataattctta ccagtagagg
    32401 ttttaagtgt aaaacgctga gcagaaacaa cgtaaggatt ccagccgcct agaggtacga
    32461 ctttaaaaag cccgttttct tggatttgtg tcagcatgaa tccgacttca tccatatgtg
    32521 ctgcgaccat tacacggggt gcatccgctt cttcatgatg gcgcagacca aaaatgccgc
    32581 ccaaaccatc taactgtaac tcatctacca gcggttccat atgactttcc atataagcgc
    32641 gaacatcttg ttcaaaaccg cttgttcctt gtaattctgt taactctttg attcgttgaa
    32701 atgttttttc ttccatatgc tattcgtcta aatttcagac taaactcctc tcagacttat
    32761 attgatctgc ggataaaata tgattttttc tgcagaccga tcgtatttat tatagcttat
    32821 tttttctggt agcggttatt tatttcaaag aaaatatgag agtgtggtaa aataagcaca
    32881 aggagtaaca agtgagaaga aggaggtatg tccacatgta tgaagaaagc gaactaagct
    32941 attttaaagg cgggttagca gttggaatcg gtctaggatt attgagcggg gtagcttcaa
    33001 cgatttggta tcatcgcaaa aaagtattgt cagcagacca agtacttgaa aatatcaagt
    33061 catcttttct aaaagaaggt gcaatcgaag gttcttggat ctcttttgaa aaggaaccta
    33121 ttaggaaatt cgccgttcgg tcgagtgctt atcggggagg tctttctcga atagaagatg
    33181 gcgaaactgt ttattatgaa tttttagcag atgctcatac aggaaccgta ttagaaattt
    33241 caagaaaaaa aggtgcaaca gattaatacg tcaaaaaagt gagagaacga atacgcaccc
    33301 actattcaaa agagaggtat catgtccttc ttttgtagtg ggtggttttt tagatagagc
    33361 tttccaaaca tatattccag tattttcttt ttaaataaat gatatttagc gggaaaaact
    33421 agcatttcct ttgattatga ggtattttta atttagcttt tgttttctat agttaaggag
    33481 agtgaaaaat ggaacaacaa gaaaaaattg ctgttctgat tccttgttac aatgaggaag
    33541 caacaatttc aacagtcatt gcagacttta aacgagaatt accagaagca gatatttatg
    33601 tctatgataa taattcgacc gatcgaacat acgaattggc cgttcaaggt ggagcaatcg
    33661 taaaaaaaga acctcgtcaa ggaaaaggaa atgtgatccg ccaaatgttc tttgatatcg
    33721 atgccgacta ttatttaatg gtagatggcg atgatactta tcctgcagaa gctgtacatg
    33781 gtttgctcga gaaattacgt tctggtgaag cagatatggt cattggtgat cgcctatcaa
    33841 atggaaccta ctttgatgag aataagagac ctttccacga ttttggaaat aaccttgtta
    33901 gaaatacaat tactagaatg tataaaacaa aaattcttga tgtcatgacc ggctatcgag
    33961 gattcaatcg tatctttgtc aaaagttttc ctattatgag ttctggtttt caaatcgaaa
    34021 cggaattgac gatccatgct ttagataaaa aattcaagtt cgttgaaatc ccaatcgatt
    34081 atcgtgatcg tcctgaaggc agcgaatcaa aattgaatac attctctgac ggattcaaag
    34141 tgatcatgat gattattaaa atgtttaaag attataagcc tttgcttttc tttgggattt
    34201 ggaccatttt cttcttttta tttggattgg tcacggggat tcctgtgatt cgcgaatata
    34261 tgctgacaag ctttattaca cgcatccctt ctgcgatttt aagtactgga ttgatgactt
    34321 tagcacttct ttcattagta acagggttga ttttggatac agtcgtcacg aacgctaaga
    34381 aagaatatga attgaatcta tatcatgttt atcatgaata ccacagtaga aagtaaaaaa
    34441 aggatcaaga ctgcaaaaat aatttgcaat tcttgatcct ctttttaatt aattatttcg
    34501 ccaaacgata gatggcatct gcatagattg ctgttgctct gaacaagtca tcaagactca
    34561 taaattcgtt tgcttgatgc attgtatcag tatatcctgg gaacatcgct ccataagcaa
    34621 cgccacgttt caacaaacgt ccgtaagttc cgccaccgat gatttgttcg tagccttttt
    34681 ctccagtgtg gtcttcatag acttgcaata atgtagccac caatggatca tccattggta
    34741 cgtaatgtgg ttccattacg cgagcacctc gagtaactgt tgcgccttct tgccccactg
    34801 tttgttcgat gtcactttgc aatccgtcta ctgttacacc ttttggaaaa cggaaattca
    34861 tattgatgaa gttcgcttcc tctgtaccat tttcttcaaa agcaaatagt ccagcattca
    34921 ttgtcaattt gcccattttt tcatcttcat aagcgactcc tagtttttct ccataaaaat
    34981 cttcatggac ataagcagct gctacatgga taaattgttt agcagatcct aggaattcat
    35041 agttatctaa gaacaatgcc aagaaagaac ctgcattgat accagattgt gggcttgcac
    35101 catgtgcgcc tttaccaact aattcgattt ttacatacgt attgtccgct tcaatcgttc
    35161 cgcttactgg atttgcttcg atgaattggt aaaatgcttc ttccatcgcg atagctgcat
    35221 ctgcacttgg tacttgaagt gctgcagttg ctgttcctgg caccatattt tcgcgaagtc
    35281 cggaaacaaa agatttcaag acataatctc ctgcattgtc gcctttgaag cgtaaagcta
    35341 aagaaacatt tcctttttca ccattgatga ttgggaattc agcatctggt gagaaaccaa
    35401 aatctggttc ttcttcatgt tcgaaataat aagccatatc gccccagccg ctttcttcat
    35461 cgctgccgac tacgaagcga actttttttg atagtttcaa gtcaagttct ttaatgatct
    35521 tcatcgcata gtaagctgcc atacttggac ctttgtcatc gcttgatccg cgtgcaaaga
    35581 tctttccatc cttgattact ggttcataag gatctgtgtc ccagccatct cctgcaggaa
    35641 cgacatccat gtgtccaaat atccctagcg tttcgtctcc ttcgccaaga tcaatatgcc
    35701 ctgcatagtt atcgacattt tttactttaa atccatcacg ttcgccataa gctaacatat
    35761 gtttcaatgc gtcgcgaggt ccaggtccaa atggtgcatc tggtgttact tttgagtcat
    35821 cgcgctcgct gttgacacgt aaaagatttt tcaagtcttc aagcaagtct tctttacgtg
    35881 cttctacttc tttttgccaa tcaattgtca taaagttaat cctccactta tttaagtctt
    35941 cgtgttcatg ataccacaaa ttacagaaaa aaatagttca aaaaatctga caattccttt
    36001 tcttatctgt ttttctctaa aaaaaatata cgctataatg agtaaaagga ggtttttaga
    36061 tgctgataaa cgaacaacta gtgaaaagag tcatcacacc tcgtccaaaa atttcacata
    36121 aaggaacatt tggacgtatc gttttgattg gcggcaatga acaatatggc gatcattatg
    36181 agtgctttag ctgccgtaca cagcggagcg ggtctcacga ctgtagtaac tgatgaaaaa
    36241 aatcggagtg ctcttcacag tcatctgcca gaagcaatga ttgtagtttg gacggatact
    36301 gaccagtttc tttccgtaat aaagcaagca gatgtactct tgattggtcc cggattgggt
    36361 acttcttcta agagtttgga acgtttcaaa caagtactag aaaaccaatc aaaagaacaa
    36421 tggctggtga ttgatggctc tgctttaagt ttacttgctg ctaatcatct tttgctaccc
    36481 tatcccgaac aaactgtact tacacctcat caaatggagt ggcaacggtt gagttctcta
    36541 ccgatacaag aacaacggga taaagaaaac aaaaagaagc aacaggaaat cggtgctgtg
    36601 ctcgttttga aaagtcatga gacaaaaatt tatggtcacc agcacttcta caccaatcct
    36661 ttaggttctg ctgctatggc aacgggcggc atgggtgaca ctttagctgg gatgatcaca
    36721 ggatttttag cccagtttcc tcaaaatgac caaacgattg cagcagctat ttatcttcat
    36781 agtttcattg gagaccagct ggcaaaagaa caatatattg ttttgccgac acaaatcagc
    36841 gaagcacttc cgaaatggat gaattatttt tgtaaaaaat caatggaaaa cgaggcatga
    36901 gtcatggaga aatttacagg ttatgtgcga gaaccttttt attatgaaac agatcaaatg
    36961 gggattattc atcacagcaa ttatatccgc tggtttgaag aggctcgggt agccttattg
    37021 gtacatctag ggtttcccta taaaaaaatc gaagaatctg ggatcatcat ccccgttctg
    37081 gaagtaaatt gcagctataa agaaatgatc cattacggag aaaaagtgag catccatcca
    37141 actatccaaa agtatacggg gacacggttg gattttagct acgaaattgt gggaactgaa
    37201 gacaaaaaat taaaaactac tggcagtagt aaacactgtt ttttactttc tgattcgcaa
    37261 cgattagtga agctgagcaa agtcaatccg gagttagatc agttattcca agcgtacgct
    37321 tctttggatg atgtaaattc ttaataaaga ccgtctaagc atttttcttg tattgtgtcg
    37381 agtagttcgt ataatgaaaa gaaaaaagca ggtgatcccc atgtatgtcg ttaaagtaat
    37441 gcacggttac atcgataaaa ctggctgtcg cactcgggaa aaaaacttag acaatctgct
    37501 cattttcaaa gacaaaaaag aatccgaagc ttttgctaaa agaatcggcg gtcgtgtcaa
    37561 accgatccaa gaggtacgac cagattgatt caatattttt cttttcgagg ctctaatagg
    37621 catgcctatc agggcttttt ttgcagactg tgtttttatg cccacacgat ttgtaagcga
    37681 tttatgttat aatagattat atctgcatag aaaggaaggt taaatggtta tcaataattt
    37741 gcaacagcaa gcttatgaat ctatacgtaa acaaatcatt tattccgagt tagcacctgg
    37801 aagtaaaatc tccgataaag tgttagaaga acaactagct atcgggcgta caccgattcg
    37861 agaagcactg atccaactgc gcaatcaaga gcttgtaaaa accattccac agtccggcac
    37921 ttatgtttca aaaatcgata tccgatctgc ttcattagcc cgttatacgc gggaaaagtt
    37981 ggaaaaaccg atcctgcaag aatgcagtgc gaaaatgacc aagcaagatc aagctaaact
    38041 agaaaaaata ctagaacaaa ccgatcaggc agtaatcgct caaaataaaa aagctttttt
    38101 tgatttggat actgattttc accgaacttg ttatgaaatt gctggaaaac gagaaatttg
    38161 ggattggcta gaaagttata gtacgcactt gaaccgtttc agatggttac gtttgacgat
    38221 ttcagaatta gactggggtc gagtattaga tgaacatcag acaatgcttc aatcaatgat
    38281 tgatcataac tttgatgaag tcggatttct ttgcacgatg catttacata tgattatcga
    38341 ggagcaagaa tacgtgattc ataattaccc cgattatttt gaagatattc aggacagacc
    38401 catcaaataa gaggttttgg aatcagcgtt ttgacctgag tattagagaa agaaacggca
    38461 aaatagttcc tcatattttg ccgttttttt tgagctaata ccgagggtca gctgattgca
    38521 caccgtttat tgagaggttg tgaaaaagct gccctgcatc aagtaataag aacagccaag
    38581 taaaaatagc ttcttatgtt tttaacttgg ctggacttat taccgcagat gcaagctttt
    38641 gaacaccgtt ttccaagagg tcgtgagcct gccgttcatc tccgagtcac aaggaacaat
    38701 ttcaaaaaac agcttctcat gtttgttgaa tattttgact tgtgaccgag gagttgacag
    38761 gagaacatcg tttattcgga aaaaaaggac tgtgacacga cttttgtcac agtctcttta
    38821 tttgtaatct aaatcatact ccgtacatct tttttctatt ttttttattt cttctaagat
    38881 cccttggcta cgaatttcat tatatggcca aagtaagact gcctctaata cataaggcga
    38941 aaaatcgctt tgattcatct cttttgaaaa actcatcgct gctatctcgc tcaatgcacg
    39001 aatgtttgat tgatgtgtat aaaaaggcaa ccgccacagc acaatttttt cgctttgcgt
    39061 ataaattccc ttaacagaag cttcgatgtg gtgaaatagt tcatgtccaa taatcaaagc
    39121 ttgcgctgtt tctttagaga tcgaagctag tcctgaacat tttaattttt ctaacggctc
    39181 cgtcatcaat cgaatccctt catcttctgc ataagttgca aaagtgattc gttcgtttat
    39241 ttgctctgat tcacaggaaa cgattggaac agccatttgt tcacaaattt ctcctacatc
    39301 ggcaaaaggg tacttttttt tgatgctaca tgccgtttcg ataccacaat cgattgattg
    39361 ttgtataaga attttctgct tggacaaagg aatctttttc caaaataaat ctcgccgaaa
    39421 ctggtagaat ccccaaattt cttcggggat ctgttgtaat tcgtttagta actcttctaa
    39481 cttatgtttg tttgatattt ccggcaacga cgatcacttc ttcctccggt tccatagtag
    39541 atacttccag atccatcaaa agctgtagtt gttccagatc cgtcgctgta aaatcagaga
    39601 ctcttgctcc taagcagaga taaaattctg gtcgggcaat caattcagtt tgataaactg
    39661 ccatatcttc ccaaagttgt tcgacagctg tcatataatt ggctgcagtt gttttcatac
    39721 atgaagcatg tccacgttgg accttgataa atcctttttg atcgatgatc cgaagacttc
    39781 cagcattttt tgaacgattc tgttcgccgc atacataaaa gaaaggtgta ttttccaata
    39841 agcaaacttc atttttattt aatcgcatat cctctgcagc aagctctagt gcttcttctg
    39901 ttgtgatctc cttggttaga tcggttgctt tcacttccgt agaaccagta gcgatagccg
    39961 ttacttttga agtctgtgga tcgatttcta cgtggacttc gatcgattct ggtgtagcgc
    40021 ctgattcaat cgctttattc atcgcttcat ttttcaacga tcgtatatct tctttacttg
    40081 gcgaagggat gatacgttct acaacatctc ggaccattgc aagtgctaca ccaatagaag
    40141 aaatcacttc ggcgttttca ggaatcgaat atttcacacc cattttatta ctaaagtaag
    40201 taatcaatga ggcagcgccg ccccctacac ctacaagaga aatctgatct ttctccaagt
    40261 gatacttgtc tgccaattct aaaatcaccg gttcaatctt agcataggat ttttccatga
    40321 tctgttctgc gatgtcttca accgtcgtat gacaataatc agctaatgct tggatggctt
    40381 ttcgtgcaga tgggacattt ccataagaaa aatgttcttc ttgcactaat ccaaggacat
    40441 tggctgcaca tgtatttgta atagtcactt cttctccgtc ttccatcact accttgacgt
    40501 aatcagcagg gtctccttct ttaggagaga aaaattcaac cttaggacct ttgatttttt
    40561 ctgtctccgt aaatactgca taatctagac cggcaatatg tgcagaacgt ggaccgacat
    40621 cgatgattcc tgactgatta gcccgtacca tcgaaccgcc tgccacacct agaacccgaa
    40681 catctaatga agagatatat gttgggtggc cacctacaat tgaataatcg attgctggac
    40741 gtccattttt aatgaccccg atattggttg ttgttccgcc tacttcaaaa taaacaccat
    40801 tagacgctct caagtacatc aaagagccca tgactgatgc tgctggaccg gaaagcatgg
    40861 ttaaaacagg tcgttttttc atttcattga tttccattac tccgccgtct ccgcgcataa
    40921 tcatcaaaga aacattgacc cctgcttctc gtacggaatc ttctgtagag gttgccgtat
    40981 ctagcatttt tggtaaaata gatgcattga ttgctgctgt ccgagttctt cttgtcaatc
    41041 catataattt tgtgatgtca gaagccatcg ttgtagggat cgattttacc gatgctgctt
    41101 cataaacaac tcgttctggt tccccattat ctactccaaa tgccatagaa gaaaccaaga
    41161 cctccgctct ttctctttct aaagaagaaa tcgtttctga aacacggtct acatttaagt
    41221 gtttgacagg taagaatgca ttgacaatct ctattttttt cttattccct aaatcgatat
    41281 cgttcaatct agtttgtcgt tttgctaaaa aaccttctaa tccacctttt gccatcccga
    41341 ttacgccgac ttttgctacg tctccttcaa tcagtgcatt agtcgcttgt gtggtagaat
    41401 gtgcaacaaa cacaacatct tctggactga tattattttc cagcaaacaa ttttgaaaag
    41461 actgaacgac acctgcggct actccacgga catcatcgtg ggttgttttc actgaagatt
    41521 ttccgatgat ttcgtgcgtc gcattatcga ttgcgactgc tttcgtatgt gtaccgccaa
    41581 catcaattcc cattcgcaca aaacgtttca ttttcttttc ctccattcat ctaatcgttt
    41641 ttatcgacgt tttgtttact aggcgttccc gtacaataag aacgtgatca ggcataagat
    41701 cgctccgatg atccaaccag ttgggatgga tttcttcata tattctcgag aagttacttt
    41761 tgtataacct aatccccaag caacccaaga ttgtgtaata tctaaatgct gaggagcaat
    41821 cgttgtgatt gcaaataacg ggaacaaaaa ggctggcgac atcgttggat taacagctaa
    41881 tacgacagct aaaatagctg agcctgaacc gactagattc attggaccgc ggaaaaatcc
    41941 tagtggtgtc aatatagcaa atacgattgc caagactaat ggtgattgcg gtatccaatc
    42001 tccaaaaata gctgaaaaat aaggagaagc atacgcagct gaattattga acatcgctaa
    42061 ggtcatcaag aaaccaacca ttggcgcaac atctactgcg ccatctgtaa ataatttcgc
    42121 aaactgacga cagatttctt tataacctcc gcgcaatttt ccagtaaaca gcaatgccca
    42181 gatacctgct agaataaaac ctaagatgat tggaagatct aaaagaacga cccctaaaac
    42241 tggtaataat acggctaacc atgagatcat tggtgcgtcg ccaccttcag ctggtacgtt
    42301 tgctgccatt gcatagcgtt tctttttgtt cattgatata ttagcgaccg ttagtaccac
    42361 aactaagctt acaatcatcc caatcatacc aatttggaaa tagtcattat aagtatagct
    42421 ttcagccgct ggattgaaac cagcataaat cgtttgatat tgtttgaaat tgacgatatt
    42481 agcaaagatt ccagccataa tcgagcccat aaatgagaac agtgcaaccg gtactgaaat
    42541 acctaatgac atcaagattg gcaacacaat aactgcaata gaaatcactg gtccgattcc
    42601 tgtcatcgac atgaatataa cagcggttac aatacatagt aagctcatcg tgattcttgg
    42661 cttgtcccct ccaagttcaa ctacctttcg aatcagggta gctgcgattc cgctatctac
    42721 tagaacacgc ccgaaaaagg caccaaagaa gacatttacc aagatcgatt tggcgtactc
    42781 agctggcccc gtcgtataga tatgcgtcaa cacatcaatt actccttgcc cttccatcgc
    42841 cgggtttggt gaaaacgtgt ttccgactag cgctaatccc atccatacag tagccatgat
    42901 ggcaaagccg accatcaagt tgtagccttt tacacagtac cagaccatga aaaagaatga
    42961 gataactaac aaaataccaa taataatatc actcatgtta ttcctccgtc cattttgttt
    43021 ttctgataag caaactaata tatcagtcaa actcattcta acttatgtag ttagcgcttt
    43081 cattaaaggg tgaccataaa tgttcagttt tcaattaaaa aaccttaatt atgctatttt
    43141 agacttatac gttattgtcc tgtttcattt gatggaggat tgtaaataaa aaaagaggaa
    43201 agaacataac ttttcgttat cttctttcct ctttataatt gtttagctta tttagccaaa
    43261 gcttcttttg cttggtcagc caatgcagta aatgctgctg catcgttaac agccaagtca
    43321 gctaacattt tacggttgat gtcgatttca gcaactttca aaccgtgcat taattttgag
    43381 tagctcaagc cattcatacg agctgctgcg ttgatacgag caatccacaa tttacggaag
    43441 tcgcgtttct tttgacgacg atcgcgatat gcgtagttgt atgaattcat tacttgttct
    43501 tttgctgttt taaataaagt gtgttttgaa ccgtagtaac cttttgctaa ttttagcact
    43561 cttttacgac gtttacgagt tactgttcca cctttaacac gtgccatgta taattcctcc
    43621 taataaaaat aaatcttcag attctgttat ttgtacttct tatttcattt ttgctagttg
    43681 ttggcgaata cgtttgaagt cgccactaga aaccatgctt gctttacgca attgacggcg
    43741 ttgttttttt gttttgccgt ggaaacggtg acttgtaaac gcgcggaagc gtttcaatcc
    43801 gcctttacca gtacgtttga aacgttttgc tgatccgcgg tgtgtttttt gttttggcat
    43861 gactatattt cctcctcaaa atctttcgtt atccgactat cggctgtcca acagccagtt
    43921 agtcattcaa attacttact gtcgtttttc ggtgccagcg tcaagaacat gctgcgtccg
    43981 tccattttcg ctttttgttc cactgttgca atatccgcag tttcttcagc taagcgattc
    44041 aagacttttt gaccaatttc tttatgggta atggcacggc ctttgaaacg gatagaagct
    44101 ttcactttat cgcccttttc caagaactta cgtgcattac gaagttttgt attgaagtca
    44161 ttcaagtcga tcgtaggact taagcgaact tcttttacat tgatcacttt ttgtttttta
    44221 cgcgcttcgc ggtctttctt ttgttgttcg aaacggtatt taccgtagtc catgattcgc
    44281 gcaactggtg gtttcgcacc aggggcaact aacacaagat ccaagtttgc ttgttctgca
    44341 atattcaatg cttctgcttt tgtcttaact cctaattgct caccatctga accgatcaat
    44401 cgtaactctc gtgcacgaat gccgtcgtta accatcatat cctttgctat ggtcattcac
    44461 ctccaaatat tatgagagaa agaaaagctt accgaataaa cggcaataaa aaaacgagtt
    44521 ctcttacaga acccgcgcgt acttcaccta atagaaccag tgaaaactat ctagcccagt
    44581 gacattatca actaaggcga gaagcgggtg cttctgcttt tatttctcaa ccatcatagt
    44641 atagcaaaaa ctaatctttt tgtcaatgtt tttcatacag tttgtcttcc ttttctgaaa
    44701 atgattagat tagcagactt tctagcagtg attcccgctt ctttgctata ctaaagaaaa
    44761 acgaggtgaa cccacatgaa aaaaatcgaa cctattccac cttctcttcc tcttcttaca
    44821 aagggagagt tttatttaga agatgaggag atcttctctg gtcttctgat caacgggggt
    44881 gaccaaagct atctcgatgc caaaaatatg gtattaagag agagtcatat ccaaaaactt
    44941 acgatgcaaa aaacagtttt acgtcatttt gaatgcagta atgtcatttt tgaaaaatgt
    45001 gatttttcca atctggagtg gattggtggc agtttccacc aagtcgtgtt tcatcaatgt
    45061 aaattaacag gtacgaattt tgccgaaagt tatcttcgag actgtacttt cactgattgt
    45121 attgcttcga tggcttcctt tagtagtaca aacttgaaag ctgtctcctt cgatcattgt
    45181 caattagaag acagcgaatt ttatgaggtc acttggaaaa accttttttt gtctgacaat
    45241 cagttgaccg gttcgaactg gtttcgaact tctctgaagg gattagactt tacgactaat
    45301 ttcttcacag caatcgcttt atctcaagat tctttggcgg gactaatcgt caaccaagag
    45361 caagcacttg tgatcgccca agcccttggt ttgcagatca aagactagat caacgaatcc
    45421 gccgccacag tgcaataagc atccaaatag ataagaccaa actgatgatc aaggcgattc
    45481 cccatccgaa aacggaatgg gtaaagggta actggagatt cattccgaag aatcctgtca
    45541 caatagttgg aacggtcaag atcagcgacc agaccgtcaa aaatttcatc gtgtcattca
    45601 agttgttatt cagcaaatta ttataagtgc cagagagctg gtctaaaatc tgcgaagcta
    45661 agttcgtcat ttcaactgct tgtcgtgctt cgatcagcgc atcgtccaac tcttcttttt
    45721 ccttttctga caacttgcga taaatagcca atgccttgat ttgttctaaa agtactacat
    45781 tttgttttgt tcctgtaact aagtaaacca aaccgatctc taaatctgaa agttgcagca
    45841 gattcttgtt cgttgttttt tctcttaatt tcagattaag ccgctgacgt tcactattca
    45901 cttcctcgat taaaggaaaa aaggaatcag aaatcaaaaa caatgtacga aacaataaac
    45961 tgtatacact catgtctggc attcgttcca aaagcgatcg aattagtcga atcacgtact
    46021 cggtattcgg cgtggtaaat gtaaacaaac cgtcttcttt taaaataaaa gcgataggac
    46081 tagtttcata atgattatcg actttttgct ggtgcggaac atttgataca agcagtaacg
    46141 cttcatcaaa agaatcgtac tctacgcgcg cccgttcatt tttatctaga gaataagtca
    46201 gcatctcgtc actaatgcca tattcttctt ttaagttttt aacagctagc acatcgtttt
    46261 gctgtacatt gatccaaaaa ttacggtctt gattaaaata gtgtttttcc cgcatactct
    46321 atcctcctga catttgtttc cagtataaag aaaaaagtcg ctcaaataaa ggtttaccct
    46381 ttatttgagc aactgtaaat caaatggcta ctttggtttg gcgaatctct gctaattttt
    46441 cttcgatcat tttcaagact tcttcttttg cttgaggatc ttccacaaaa ttatagcggt
    46501 ctccatcgat ttggattttt ggactttctt tgtattcttc ataccattga tcgtaacgtc
    46561 tgttcaattc tttgtaataa tcgtatagac ttggatcaaa ggacaattgt tcatacgaac
    46621 gaccacgttt ttcgatacgc tccaacatcg tatcaaaaga tacacgaata tgaactaata
    46681 aatccggatg ttttttatgg gcagcatagg gaagttcttc catcatgttt tccagcagtt
    46741 catcatacac acgaacttct gtttctgttg ctcttcctag atctgcattt agatggaaaa
    46801 gcagtgaatc ttcataaatc gaacggtcca atacattatt gtcatctttc attgcttgtt
    46861 tgatgcttgc aaaacgttta ttcaaaaaat aaatttgcag caaaaaagca tactgttcag
    46921 ggtttgcata aaataagggc aagacttcat tatcatcaat cgattcataa aatgcctgac
    46981 tatcaaagtg ttcagccatc atttctgtta aacttgattt tccggcacca attgtacctg
    47041 ctaaaacgat cacgctcatt tacctccagc tcacttcatc actaaatatt gtgtttaact
    47101 ccaacctttt taaatatacc cccaatatct agtagtgtca attgtatttt tttctagtca
    47161 aagaagaaaa aaagtcaaaa aattcttaag aaaaaagtgt tttttcttta gaaaacgtat
    47221 tcttagtttt acttctgaga tggttttagt aaaataaagg aggaatgttt tttgagaccc
    47281 ttagtggaca aaaaaattcg tgaagcagcg caactcgttt tgggcgcttt attttttgct
    47341 taaaaatcac ttttacgtga gaaggagtta gattatggca aactatggcc aaggagaatc
    47401 aagcggaaag atcatattga tgggcgagca cgcggttgtt tatggagaac cagcgattgc
    47461 ctttcctttc tatgcaacaa aagtcaccgc attccttgaa gagctggatg caatggacga
    47521 tcaactggtt tcttcctact attcaggaaa tttagccgaa gctcctcatg cattaaaaaa
    47581 tatcaaaaaa ttattcattc acttaaaaaa acagcatgac atccaaaaaa acttgcaact
    47641 gaccattgaa agcacgattc ctgctgaacg tggaatggga tcaagcgctg cagtcgccac
    47701 agcagtcact cgtgcttttt atgattactt agcatttcct ttgtctcgtg aaatactatt
    47761 agaaaatgtc cagctttcgg aaaaaatcgc ccatggtaat cctagtggaa tcgatgcagc
    47821 cgctactagc agcttgcagc cgatttattt tacaaaaggg catcctttcg actacttttc
    47881 tttgaacatc gatgcttttt tgattgtcgc tgatacagga atcaaaggac aaacaagaga
    47941 agccgtcaaa gatgtcgctc acctctttga aacccagcct catgaaactg gacaaatgat
    48001 ccaaaaatta ggatacttga cgaagcaagc aaaacaagca atcattgaaa attcaccaga
    48061 aacgttggca cagacaatgg atgaatcaca atcacttctg gaaaagctga caatcagcaa
    48121 tgattttctt aatctattga tccaaacagc aaaagatacc ggggcattgg gtgccaaatt
    48181 aactggcggt ggacgcggtg gctgtatgat tgcattagca caaacaaaaa caaaagccca
    48241 agaaatcagt caagcacttg aagatgcggg tgctgctgaa acttggatac aaggattagg
    48301 agtacatacc tatgtttaaa ggcaaagcac gcgcatatac caacattgct ctaatcaaat
    48361 attggggtaa gaaaaatgaa gaactcatcc ttccaatgaa caacagcctt tcgttaacat
    48421 tggatgcatt ttatacagag acagaagtca tcttttctga tagctatatg gtagatgaat
    48481 tttatctaga tggcaccttg caagacgaaa aagcaacaaa aaaagtcagt cagtttcttg
    48541 acctttttcg taaagaagcc ggcctctcac taaaagcttc agtaatcagt cagaatttcg
    48601 ttcctaccgc agctggatta gcctcctctg ccagcgggct agctgcttta gcaggcgctt
    48661 gcaatactgc tcttaagctt ggattagatg atctctctct ttcaagattt gctcgacgcg
    48721 ggtctggttc agcttgccga agtattttcg gtggtttcgt cgaatgggaa aaaggccatg
    48781 acgacttaag ttcttacgct aagccagtcc cttccgattc tttcgaagac gatttagcaa
    48841 tggttttcgt tttgatcaac gaccagaaaa aagaagtgtc cagcagaaat gggatgcgtc
    48901 ggacagtcga aacatccagt ttttatcaag gctggttaga ttccgttgaa ggggatctat
    48961 atcaattgaa acaagcaatc aaaacaaaag atttccaact tctcggagaa acgatggaaa
    49021 gaaacggact aaaaatgcat ggaacgactt tagctgccca gcctccattc acttactggt
    49081 ctcctgacag cttaaaagcg atggatgcgg tgcgacaatt gagaaagcaa ggcattccat
    49141 gctactttac gatggatgct ggccctaatt tcaaagtttt agtggaaaac agccatttat
    49201 ctgaggtaca agaaacattt accaaattat ttagcaaaga acaagtaatt actgcacatg
    49261 ctggtccagg aattgcgatt attgaataaa gggagatttt tatgattgaa gtatctgcac
    49321 caggcaaact ctatattgcc ggagaatatg cagttgttga aacaggccat ccagcagtta
    49381 tcgctgcagt cgatcaattc gtgacagtca ctgtagaatc tgcacgaaaa gtcggaagta
    49441 tccaatctgc acaatatagt gggatgcctg tacgttggac aagacgcaac ggagaattag
    49501 ttttagatat tcgggaaaat ccttttcatt atatccttgc tgctattcgc ttgactgaaa
    49561 agtatgcaca agaaaaaaac attcttttat ccttttatga tctgaaagta acaagtgagt
    49621 tagacagttc aaatggccgg aaatatggat tgggttctag cggtgccgtg accgttgcaa
    49681 cggtcaaagc cttgaatgtt ttttacgcgt tgaatttatc tcagttggag attttcaaga
    49741 ttgcagcact agccaattta gcagttcaag ataatggttc ctgcggcgac atcgctgcta
    49801 gctgttatgg tggctggatc gctttctcaa ccttcgacca tccttggctc caagaacaag
    49861 aaactcagca ttctatcagt gagttacttg ccctggattg gccaggtcta tccattgagc
    49921 cattgattgc tcctgaagat ttacgtttat tgattggttg gacgggtagc cctgcctcta
    49981 cttctgattt ggtcgatcaa gttcaccgtt cgagagaaga taaaatggtg gcttatcagc
    50041 ttttcttaaa aaatagtaca gaatgtgtca acgaaatgat caaagggttt aaagaaaata
    50101 atgtaacgtt gatccaacag atgattcgaa aaaaccgaca attactgcat gatttatctg
    50161 caatcactgg ggtcgtcatc gaaacgcctg ctttgaacaa attgtgtaat ttagctgaac
    50221 agtatgaagg agccgcaaaa tcttctggtg caggtggggg cgattgcgga atcgtaattg
    50281 ttgaccagaa atctggcatt cttcctttaa tgagtgcatg ggaaaaagca gaaatcactc
    50341 cactgccgtt acatgtctat agcgatcaaa gaaaggaaaa ccgatgaatc gaaaagacga
    50401 acatgtttca ctagctaaag cctttcatga taaacaaaaa aacgaatttg acttcgtacg
    50461 gatcatccat aaccctttgc cgcaaatagc agtttctgat gtcgatctaa gtacacaggc
    50521 agttgggttt acactaagca gcccatttta tatcaatgcg atgactggcg gaagcgaaaa
    50581 aacaaaaaaa atcaaccaag atcttgcaat cgtcgcaaga gaagcagatt tgatgattgc
    50641 cactggttca gtcagcgctg ccttgaaagt cccttcttta gccgatactt atacgattat
    50701 gcgacaagaa tatcctcacg ggaaaatcat tgccaacatc ggtgcaggga cttctgtcga
    50761 aagagccaaa gaagctatcc gattgtttca cgcagatgcc ttacagattc atttgaatgc
    50821 acctcaagag ttggttatgc ctgagggaga ccgtgatttt accaattgga aagtacttat
    50881 tcaagaaact caaacagcca ttgacgtccc cctcatcgta aaagaagtcg gttttggcat
    50941 gacaagagaa acgctcaatg atctagctgc tttaggagtt catacagttg atatcagcgg
    51001 tcgaagcgga acaagcttca cccagatcga aaacgcccgt cgctccaagc gggaactgag
    51061 ttatttagct gactgggggc aatcgacagt ctcttcattg cttgaagcaa atgaagcaga
    51121 cacttctatg gaaatcctag cctccggagg tatccgcaat gcctatgaca tatttaaagc
    51181 cctttgtctc ggcgcaaatg ccgtgggtac ttcaggaacc gtactgacgc atctgatgaa
    51241 tcacggcgta gaagaaacta ttatactgat gaagcaatgg caagaagagc ttcgtctgct
    51301 ttatactatg gtcggcgcaa cgaataccgc ggccctacac caacaatctc tgatcttttc
    51361 cggaccagtg aaggattggt gcgaagcaag agggatcgac ttagtgaagt atggaagacg
    51421 cacagaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta
    51481 caaacggcgg aagtaaaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa
    51541 aagcaatttt cgtgaattcc ttcttatttc ttggagttaa atgcttctgt cccagcctct
    51601 tttttcgaat aaatgatgtt taatcagctg tccttcggta ttcgttcaat ccgctgattt
    51661 cacaacctct ccactaaaca gtgtactaaa gctcttttaa ggttgttttc cgatataggc
    51721 aagtatgcct ccgtccacat acaagacatg gccgttaacg aaatcagatg cgctagaagc
    51781 caagaagaca gatggtccag ccaaatcgat tggctcgccc cagcgtgctg ctggcgtacg
    51841 tccaacgata aattgatcga atggatgacg ttcaccgttt tcttgtgttt cgcgtaatgg
    51901 agcagtttgc ggtgtagcaa tataacctgg tccaattcca ttacattgga tattgtattg
    51961 accgtattca gaagcgatgt ttttcgtcag catttttaat ccgccttttg ccgcagcata
    52021 agcactgacc gtttcgcgtc ctaattcact catcatggag cagatattga tgattttacc
    52081 gccgcctttt tcgatcatat ccggaataac tgcttttgaa acgatgaatg gggcatttaa
    52141 atccacgtcg attacttggc ggaagtctgc cgcatccatt tcgatcatcg gtgtccgttt
    52201 gatgatccct gcattgttga ctagaatatc aactgaacct acatcttctt tgatctgttg
    52261 aaccatcgct tgaaccgctt cttcatcggt cacatcgcag acataacctt ttgcatcgat
    52321 gcctgcttct ttataatgcg ctataccttc atcaactgat tcttgattca agttattaaa
    52381 tacgattttt gctcctgctg tagctaaaga gcgtgcaatc tcaaatccaa taccatagac
    52441 agcaccggtg actaatgcta cttttccatc taaacgaaac atatccatac taaattccat
    52501 cattttctct cccttatgct tattttaatt gatccatttg caccatatcc atgtctgtat
    52561 aggtgatatt ttctccgcac attgcccaga tgaatgagta attgcttgta cctacacctg
    52621 aatggatcga ccagcttggc gaaataatgg cttgttcatt gtctacaact aaatgttttg
    52681 tttcatctgg tttgcccatc atgtggaaca cacgtgtatc ttcattggca taatcaaagt
    52741 agacataagc ttccatacga cgttcatgtg tatgacaagg catcgtattc caagcgcttc
    52801 ctggttctaa aatcgtataa cccatttgta attgacagct ttcgcataca tttggatgga
    52861 tgtattggta gattttacgt tcattcagtg tcaggccttc accagtctcc attggtttta
    52921 cttggtcgat actgattttt acatttggat atttatgatg agctggaaca gaactaatat
    52981 agaatttagc tgggttttca gcatcttgag aagaaaatac aacatgtcgt gtttcttttc
    53041 cgatatagta tccgtcttgt tttttcattt cttctttttg cccatcgatc tcgatgaatc
    53101 ctggtccacc gatattgatc acacctagtt cacgacgctc taagaagtaa tcaacaccta
    53161 gttctttgtc taatgtgatt tctaattctt tttcagtagg tgtcactcca ccaaagatca
    53221 ttcgatcgtt gtgcgtataa gtcaaactga ttttccctgg ttcaaatacc ttttctacta
    53281 aaaattcttt tctcaattgt tccgttgaat aatgacggat atcttctgga ctgtgtgtat
    53341 aacgtgtttc catttcttgc attttcttct actccttcta tcgaatgatt tttccagagc
    53401 cgcatgctaa aaattgtgcg acttcttctt gactaaattg gttgcagtcc ccatggatcg
    53461 tatgcttcaa ggcagaagct gccgtagcaa aatcaatgat ttcttgcgga gacatattgg
    53521 ataaaatccc atggagcacg ccacctgcaa aagcatctcc gccacctact cgatccacga
    53581 ttggggtgat tctatggact tgggattcca catattcatg gttcatccac aatgtcccct
    53641 tcaattcatt ttcacttgct gaatgaacgg tacgcttggt cgaataaaac acctgaatat
    53701 tcgggaactt ttcctgcatt ttttgataat aataaattag aggttgttct tctgcttctg
    53761 gcgctttctc gatacctaac aaataaagag catccatttt tccagctgag cagatatcta
    53821 ctaaaggtaa aagctgatgg attacttcgc tggcttcttg ctgtgtccat aattttccac
    53881 gatagttgat atcgaagctg atcaggcagc cagctttttt tgcagcttct atcacctgtt
    53941 tcgtcatttt ttgccatttt ttagacaaag ctggggtgat tcctgaaaca tgaaatatat
    54001 ctattccttg gaataactcc tctagcgacc attccaaatg atccatttca gcaaaacttg
    54061 atccggcacg atcatagatc actgaggcgg cgcgttcgcc actaccagtt tccatataat
    54121 aagtacctag tcttgatcca ccagataata cctgtttcgt atctactcca taacattgca
    54181 agtgcttttt gactgctttt cccaatgcgt tatcaggtac ttttgtagca aaaatcgctt
    54241 catgtccgta attagctaaa gaaattgcta cgtttgcttc ccctccgcca taatgggcag
    54301 cgaatcgttc acttgaatcg atacgggttc cctgctctgt ggacaatcgc aacatgattt
    54361 ctccgagtgt cactactttt cccataagca gctacctctt gatttctttc atttttgctg
    54421 catactgttg cgcgacttcc gttactttgt caaaatctcc tgtagctgcg ggtgctaaaa
    54481 ggttcccacc tactccgact gttacgacac cagcatcgaa ccactctttc atattgtcca
    54541 gactcacacc gccagttggc atgatattga cttgaggcat cggcgccttg aaggcagaaa
    54601 tgatacttgg tccataagcg cttccaggaa atagtttgac gatatctacc ccgcttttca
    54661 gcgctgtttt gatctcggta atagtcatac atcctggtaa gtaaggaatt tgatacagat
    54721 tgcacatctc tgccgtttct tgatcaaagg aagggctaac aatatactct gcgccagcta
    54781 agatagccaa gcgagctgtg acagcgtcta atactgtgcc tgcgccaatc actatttctg
    54841 gttggtcttt gtagaatgac aaaagttctt tgattacttg atctgcttga gggactgtaa
    54901 aagtcaactc aataccagtt agaccacctt tgatgattgc gtggcaagca ttcaaagctt
    54961 cttctttgct ttttccgcgt accaccgcaa tcactccggc gtttttcaat cgagataaaa
    55021 tatcaatttt tttcatcttc tccacctatt tcctatttag aaattttatt ttataacttg
    55081 gaaatctata cccatattat acggcagaaa agaaaaattg caaacgcttt tttcattatt
    55141 tttgaattct ttgctcgatt tcgctttttg tttgcttgag ggcactgatt atttgttctt
    55201 ttgtctcatc tgttaaacga tatttcggca tacttacact aaatgcacca atgatttgtt
    55261 cgtttttcac gattgaggct cctatacaaa aaatatcacg ttcgatttct tcatcgtcaa
    55321 atgcgatgtg ggtttctttt accgttttga tttccttttt caaccgtaat gcattggtga
    55381 tcgtatattc tgtgtaaggg atcaacggat gcttttcaag ataatcagtg tactgctctt
    55441 cactgaattc tgctaatacg gctttgccca tcgcagaatt gtataatggt cgggtgattc
    55501 cgatttttga agacatgcgg atcgtttgat gttttggatc tagtttgttc acgtacaaga
    55561 tctcattgtt gtccaatacc cctaagtgga tagtctcatc gatcttttgc tgcagctgtt
    55621 ctaaaaacgg taaagtcaac tctaccaaat cgatttgttc aatgttttta ttcgcgtagc
    55681 gaataagctt tgcacctaaa cgatagtttt tctctgaatt tttatttacg tagccgatca
    55741 tcaacaaagt atctagtatt ttcaagactg tagaagaggt cattcccaca ccctttgcga
    55801 tcgtctgcaa agagacatct tggttttccg ctaaatagtc tagtatttta gaggctttga
    55861 tcaatacagt tccataaggc tttttatttt ccatataata ttctcctatt tctttataag
    55921 aaaaattata gcggaaatac catcaattta aaagagatat ccagatataa actaccattt
    55981 ctctttacag gcaatttaat gttgaagatc cgcttcgttt ttacatcaaa ctttgatata
    56041 aagcaatgat ttcttctact gtcgtttctc ttggatttcc tggtgtgcat acatctcgca
    56101 aagcatcttc tgcaattgct ggaatatctg cttcttttac acctaattca gaaatggttt
    56161 ctgggattcc aaccgctttg ctcaaccgat cgatctcacc acaagccgct tctcttacat
    56221 cttctaaaga catttcagct gctcctttaa tccctagaac aagagcgatc tcacggtact
    56281 tttctccagt atattctttg ttatatttca tgacagttgg caaaagtgct gcacacgcta
    56341 cgccatgagg aatgttgtac catgcactta acgggtgagc catcccgtgt accagtccta
    56401 aaccgacatt tgagaagccc attcccgcaa tatactgtcc taatgccatt ttttctcgcc
    56461 cattttgatc gccatctaca gaagcttcca aagaatggcc gatgatttcg attgctttta
    56521 aatgcagcat atcagtcatt tcccaagcgc cttttgtgat aaacccttca atagcatgcg
    56581 tcatagcatc catacctgtt gatgcagcca attttttcgg cattcccatc atcatatcgc
    56641 tgtcgatgaa cgcgacgatt gggatatcat gaggatcaac acaaacaaat tttcggtgat
    56701 ttacttcatc tgtgatgacg tagttgatcg ttacttctgc tgctgttcca gaagtcgttg
    56761 gtatagctaa tataggtaaa catggatttt tcgtatcagc aacaccttct aaactaatga
    56821 cgtctgaaaa ctctggattc gtcaaaataa tcccaatggc ttttgccgta tcgattggcg
    56881 aacctccacc gattgcgatc aaacagtctg cctctgcctc tttaacaaaa gccactccat
    56941 cttttacatt ttggatcgtt ggattaggta cgatcccgtc gtataaagca taagcgattg
    57001 ctgctttatc tagaagttct gttacttttg ttgctatacc gtgctcgatc aatcctttgt
    57061 cagtaatgac agctgctttg ccgaatcctc gctgctgaaa ttcagggacg atcgactcaa
    57121 tcgctccttt tccaaaatac gatgtttcat ttaaaatcat tcgattactc attttctatt
    57181 cctcctaatt tgtattattt ttcacaataa aaacgaaaga accccaagca accggttgcc
    57241 cagttgcttg ttgctacagt tcaaagactg gcatcaattc ccaactgact ggagaggcat
    57301 ctggatttgt ctccatgata tcttccatgt acttccacca tttttgattg atagatgtat
    57361 ttgccatttt ttcccataac ttttcatctt cgatcactaa gtaaccaaat aacaaacttg
    57421 tactcggatc aagatagatc gaatagtcga tgaccccgtg ttcacgaagt gctgtacgca
    57481 tttctggcca aagttcatca tgtctttttt tgtattcttc ttgtttttct gggtaaactt
    57541 tcatgcaaaa agcttttttc accatgtcag acacctgctt tcgtatgaac ttcctccaaa
    57601 taacctgctt ttggagtgac accaaacgca tctgctaaat gccacaggtt ttcgtccgtg
    57661 attgtttgca agattggtcc ttgcgctttg acgtacgtat agacttctgc tgccttttct
    57721 gctgtctcga tcaaaccaaa gacttcatcc atgtcttttc ctgacccgta gattccatgt
    57781 tgtggccata aaaccaatcg agtttctttc attttttcag ctgttgcttc cccaatctca
    57841 tttgttcccg gaaccatcca cggaatgatg gaaattcctt ctggaaaaac aactaagcat
    57901 tccgtacaca tttgccatag cgtacgacta aaggaacgtt cgtctaattc atgcgtaaaa
    57961 ctcatcgcta atagatgact agcatggcaa tgcataacga tccgattatc tgggtctact
    58021 tctaaacggg cgatatgact catgaaatga cttggcaact cgcttgtagg aaggcattcg
    58081 ttttccagcc cccacaaaag ttccagtgtt ttgccatctt cagcaactcg aacgattcca
    58141 agattctcgg caggttcgct cgctacattt ttaaaatatc ttcctgaacc agttacaata
    58201 aaatagcatc ctgctaaacg gctggcatca aaaatcatcg agatgcggcg gatcactttt
    58261 tcaggatcca agaacggtgt gatctcttct tcttttagta aataagaaat attcccgcca
    58321 tttctttcat cccatcccaa gcgataaaga ttcgacgtcg tttcgatcat ttctttcaca
    58381 taaggcgctt gtaaaatatc tatttttttc aatatagcca cactcctatt ttcttaattc
    58441 caacacgtct ttttgatatt cctggacttc ttccagccaa tccaaaccaa ctggaacttg
    58501 atttttgtaa cagaaataat tccatacatc ggcatacggg aaatctttca attcttccgt
    58561 ataagccatt cttgaagtaa agtcaaaagc cagctccatt tctttcaatc gctcaactgg
    58621 ttccagcata gctttcagca gtgctttttg tgtatttctc gtaccgatga cccaagcagc
    58681 gatacggttg atcgttgcat caaagaaatc cagaccgata tttgtttttt ccaacaattc
    58741 gttacggact aattctttag cgatttcctg caattcatca tccatcaaaa ccacatggtc
    58801 tgaatcccaa cgcactggac gggaaacatg caacatgatt cctttgctga ataaagctaa
    58861 tgaagataat ttattggaaa tcacttctgt tggatgaaaa tgtccagcat ctagacaaac
    58921 aagtttgtca cgagtcaacc cgtaacccat ataaaattca tgggaaccga ctgtataagc
    58981 ttcagcgcct aaaccaaata gtttgctttc tactgcatct tgtacatacc gttcatctaa
    59041 ctcttctgta aaaatttcat cgagtgattc cattaaacgt tcccgtgggg taaaacgatc
    59101 aatcggatta tctttcatcc catcaggtac ccaaaagtta ttgatacttg tttgcccagt
    59161 ttctttccca aagtactccg cgattttgcg gcttcttttc ccgtgttcga tccagtattc
    59221 acgaacttct ttatctggat gagccaatgt aaagccgtct ttcatcattg gatgcgaaaa
    59281 gaaagtcgga ttgaagtcaa gaccgatttc ttcttttttt gcccactcta cccatttttc
    59341 aaaatgtttc ggctcaattt catttagatc gacagattct tctgtatcca aatagatagc
    59401 atgaaggttc aatttgtgtt tgcccggtat caacgaataa gccttctcca gatcttgtct
    59461 aagctcttca ggtgttcgtg cggctcctgg aaaattccct gtcgccatga ttccaccggt
    59521 caattcgcca tccggtgtca agaaaccttt gacatcatct ccttgccagc aatgcataga
    59581 aattttgatg tcctgtatct tttctagtac agcatctgta tctacaccga tagttgcata
    59641 tttttctttt gcttgttcgt aatttctttc aatcgtgttc atacgattac tcctttgatt
    59701 ttattcacgt accattccat cgtgttttgc ttattttcat ctgggtaaaa agtttcacat
    59761 ggaaacgact gtttgattac atttcttgct tgttctatat tcgaaaactg acctgtactg
    59821 agcatttgca tggcaacatt cccgattgct gtggcttcac taggtcctgc ttctacaatg
    59881 attccggcat aatctgctgt caattgattc agtagacgat tattcgatcc gccccctacg
    59941 atgtgcagct tttccaattt cgttccagtt aagtgttcta ctttgatcag ctcttgtgcg
    60001 taacaaagag caagattatc atacacacaa cgagcgatag ctcccgatga gacagggact
    60061 ggctgctctg tttcaaaaca gtattgctga atctcttcta tcatatttgg gggattcaaa
    60121 aatcgagggt cattgatttc catcacttgc tgcagcggtt tttcttttgc agcaagctct
    60181 gccaattccg cataactgta ctcgtactct tgcattcggg caacttcctg gatcaaccac
    60241 atccccatga tgtttttcaa aaaacgcacc gtgttatggg caccccattc attcgtataa
    60301 ttttcagaaa aagcattgcg agaaacattg gcgatctttg tttcaatccc taaaagcgac
    60361 catgtcccac tgctgatata accccaacgt tctccttgac ctggcgttcc taatacagct
    60421 gacgccgtat catgacttgc tactgtgata aaggtcacat ctggcaaatc atattttgaa
    60481 aattgttctt tttgcagatc tcctagtatc gttcccggct ctgtcaatgg tggaaataat
    60541 tccttcgaga caccgatttt ttcaagaagt tctatttccc attcttttgt ccctgcattc
    60601 agcagctgca tagtagatgc attagttttc tccgtcacca tgttaccagt aaagacatac
    60661 cctaaataat caggtatcag taacaattta tgtgcttttg ccaaattttc tttttcttca
    60721 gcaaacaatt ggaacaaggt attgaaaggc tggatctgaa tccctgtctg ctcatacaac
    60781 ttatctagag aatagatttc agaaaactgt tctactgctc cattggtacg cttgtcccga
    60841 taagcaatcg gatcatttat ccgatcccct tgttcatcaa tcagacagta atcgactgcc
    60901 catgtatcga tccccgcaaa aaggcgatca atccctgtct gctttaattt ttcaaatcca
    60961 atcaaaatct ctttgataag ataatcgatc ttccagcgat cgtgaccgtc tttgttccga
    61021 aagccgtttt tgaaccggtg tacttcttcc aagatcaaag aaccttttgc atccctcgta
    61081 cttttcatta gtcgtccgct tgatgcaccg atatcgatgg caacaaatgt tggattcatt
    61141 tctccacctc ttttacttta ttgttgatta gcactttatt aataaagcgt tatcatttat
    61201 tgacaatgct atcataatac agaatcttat attttgcttc acactatttt ggtatataat
    61261 tgcattatct tgacaagttg ggagtgttta catggattac tgggaaaaag ccaaccactc
    61321 atggtcggaa gactcaaaaa gagtgatctt gacgcctaca caaagaagca ggaatttatt
    61381 tttttatatc caagaaatcg gttacttcaa agcaccaatc ccttactata ctgaacgagc
    61441 acatttacct tcttacttga taaagtttac tttgtctggc aaaggttcgc ttacatataa
    61501 tgataatcag caacagctaa aagctggaga cttatttttt attgactgcc aagaatatca
    61561 gcgttatcaa acgatcagcg aagaaccatg ggaaatggat tggatccatt tgaatggtca
    61621 gaacgtccaa tcattttatc aggagttcac aaaagacggc cataacacct tccattcaac
    61681 aggagatcca tcagaaaaca agatccacca tttgatcaca gaattgatcc acatgcaaca
    61741 aggcacgaat gcccgtaccg atttccagtc ctctgtagct atccacgagt tgctgaatga
    61801 actgattttg caaaagtatc agctagattt cgaagaagag gatattcccg cctacgttta
    61861 caaactgaag caatacttag acgaacattt caaagaaacg attacacttg atcaattaga
    61921 acaacaattc ttattgaata aatatcagct gagcaaagat ttttccaaat ttatcggttc
    61981 tccaccaatc gattatttga tcagcaaaaa actttcttac gctaaagacc ttcttcggta
    62041 tacagaattt acggttcgaa tgatttccga agaaataggg attgagaatt ttgcttactt
    62101 tagccgcctg ttcaaagcaa aaacaggact atcgccgaga gactttcgta aattcagctg
    62161 attcatataa tatgtacgtc attttttcaa aatattttga aatatataga taaaaataaa
    62221 ataaccgcag tttctgcttc tatgtaaaca acagcagcta ctgcggttat tttttagtga
    62281 tcattctcat tgttcatctt caaaatgttc ataatgaacc aactgtttct gtgtatcacg
    62341 tttcaaaaat tctttaggcg ttatgccgaa ctctctcttg aatgctttca gaaaatgcgg
    62401 ataagaagaa aaaccacttt tactatatac agcactagag ctgcggtatt ctcgcaacaa
    62461 gtgcttggca tagatcagct ttttcttcat tacatactga tgaaacgtaa atccagtgta
    62521 ctttttaaat tctcgtgtga tatggtattt gctgacaaaa aactgctttt ccatttcctc
    62581 taaagaaagc ggatgagata aattttttga gatatattgt aacatttgtt ccacacgctc
    62641 attaggaact acagcattct gttcttgcgc actctcttct ttttgtacaa cttgattgag
    62701 aaaaatcaag tattctacca gagcttgttc atattttatg tcttcaccgt aaattttttt
    62761 atcaggagga tggtcgataa atgataattt ttgcttcaat acttcctgtt ctatttgcaa
    62821 tacacgactc ctcttctctc ccattggttg aaaacaagcc gatagattag accatggcgt
    62881 ggaacgactt tctagaaaag aaggggtgac aaagatatag acacgctcga atacatctga
    62941 agattggcgg acgatacgat gcaaatcatt ggaatggatg agcagcacac ttcctgcttt
    63001 gatcgtaaat tgtcgtccgt tcaaataaaa agtcgcttct ccctttaatg tagcatggat
    63061 ctcatagaaa tcatgatgat gatagatcgt tttattttgg ctgacgacat ctttgacatg
    63121 atagatttca tactgatcag aaaccatcgt attgacttgt tcaccgctga ttttcttttg
    63181 gagcatgtgc cgcacctcct tttgttcact tatgcacaat tttattatac cacgtttttt
    63241 gtaatcaaaa agagctgctt ttatatactc ttataaaaaa gaagagacgt ggcatcttct
    63301 agccccgccc cctccattct aaatattagt taaccaacgt cttctttaca ttattcaaga
    63361 agacttcttc cataacgaat cccatcatct tttgtttctt caagaaccac atacaggtac
    63421 ggacgattct tcatgatgat ttctttgatt tttgtgtact cgatcacacc gtctcccaaa
    63481 tttaccggca cgattttttc ctgttcgaca cgaaaatctt tcaaatgaac cgcacatatc
    63541 ttttcaccaa aacgttcaaa agcttcttct accaattgga cttgttcttg ataatcacta
    63601 cttgtgatca aattggtcgg atctaaaatg attcctagat agtccgaatc tactgcttgg
    63661 atcagctgct ctacccttgc taaagaatac accggatgat tcaaacctgg ttctatcccc
    63721 accatcattt ggtatttttc tccagctttt accaagcgac ggataacgga cacagcttca
    63781 gcaaatgctt catctgtgaa attttcttcc gtatattgga tttccggcaa aacacatcca
    63841 gtttccgaag caaccatcga cgcaccaaag gaagaagcat agcgaagata ctgctcgaac
    63901 tttgtcagca actcttgtcg tatcgttaaa tctggatgaa tcatattgat atagcaactt
    63961 aaaattccta cggtcacttc ttgcttttct aaaactcgtt tgacataatt tcccatccca
    64021 gaattgatct catttgcgcc agaaggtatt tcaggaaacg aaagacctaa ggcgagctgc
    64081 agtgtatgaa tcccttgttc ttttgttttt tctgcaagat cttctatcgt ttggacttcg
    64141 gaaagatcat gtcctctaat tgtaatgttc aacataacta ttctcctaac tcagcatatt
    64201 gggtagccac atgacgattt gcgggaagta agtaatcaaa aacaagacga ctaagataac
    64261 cccataaaaa ggaagaagcg gtttcagcat tgcctctgct tttacgcctc ctacacttgc
    64321 accgacatat agtcctgtac ctactggcgg cgtgatggag cctacacaca gattcatgat
    64381 tgaaaacaac ccgtaatgta caggatcaac ccctacactt gtgacgattg gtaaaaatat
    64441 tggtgtaaaa atcaagatag caggcgccac atccataaac atacccacca gtaataacac
    64501 aagattgact aataacagaa taataaattt attatccgtt acgcccataa tcaacgaact
    64561 aattgcctga ggaataccgg taaacgccat ggcgaaagac atcatggaag aagtagccaa
    64621 taagaacatg atcgtccctg acattgctac tgattgaaac aacatattag gtatatcttt
    64681 tagttttacc gttttatagt aaaacatagc aatcaacagg gagtaaatga cagctatcgc
    64741 tgaagcttct actgctgtaa atatccccgt caagatacca ccgataatga tgacaatcaa
    64801 tagaaggcta ggaatcgcct cttttattat ttttccagca tgagccatct gtcctttttc
    64861 gacaacaggg tatttattct ttttagcaac gatgaatgct actagcatga ttcctagcgc
    64921 ccacaaagcg cctacgagat aacctcccat aaacaaagca gagatcgaag caccaccagc
    64981 aacaagtgaa tacatgataa aagcagtact tggaggaatg accatacctg tcggagctga
    65041 cgcgatattg accgctgtcg caaacttccg gtcatatcct tcttccactt gctgagggat
    65101 caacactccg ccaatcgcgg tagaagcggc aatggccgag ctagagatgg aaccaaataa
    65161 agcattccct aacacattcg tatgagccaa agcccccggc acgcgcccga cgaacaacat
    65221 cgcaaaatcc actagtttct tagctatccc tccattattc atgataatgc ccgagagaac
    65281 gaaaaacgga atagcgatca gcgaaaaact attgatactt cccaccattt tttgagctga
    65341 actaaaagca gaaacatcaa acggtataac taacaataaa gtcatcatcg atgagaatgc
    65401 aatacttata gcaatcggca tccctaatac aagcaacaca acaaatacaa aaaataacac
    65461 taatccggct tctattgcca ttttattcct ccatttttat attttttctt gaattgctgg
    65521 cttgttgctt aatgtatcga ttagattgat caggctatag ataatgatga gtacccccga
    65581 tacaggtact gacagataaa catatcccat aggaatactt aaagaaggcg agatctgccc
    65641 cattgtcaat gtgacagcat tccatccgcc atacaccatg atcatcgcag caaataccag
    65701 aaataaactt tgaacaacaa cttctaaaat tttacttttt ttgccagtta atttttcact
    65761 taaaaccgtt acggctaaat gtgctttttg tcccacaaca tacgcggaag acaacatggc
    65821 aaaccagaca agaccgaatc gaacaagctc ttccgtgacc gtatttggat ttcctaaaac
    65881 cgttctggaa aatacttgcc ataaaacaac agcgaccatc ccaacaagca aaacagctcc
    65941 caggatctct aataccttgt ttaaaatcat tttcatcaca gctacgctcc ttgctcttgg
    66001 atcttttgat aaaggtcttt gtaggtagga tcgtttttga atatatcatg aagtggggca
    66061 actgcctctc gaaacggtgt attatctaca tcattgaatt ggactccatg ttcttcttgt
    66121 gctaaagctt tttcttcttc aattgctttt gcaaaagctt caatttcaaa aaccgttgat
    66181 tctttcgctg cttcttcaat ggctttttcc tgctcatcag ttagctgttc tttgatggca
    66241 tcattcatga tgatgatatc tggcacacgt gtatgctcat cataggaata gtatttagcc
    66301 acaccaccat gtccagctgt atacaagaca aattcattgt tttctgatcc gttgatcaaa
    66361 ttggattgaa gagacgtgta cacttcatca cttcccatag gtacgggcga accgcctaaa
    66421 agctctacca ttttgatagc ggtttcgctt tgcatcacac ggatcttctt tcccttcaaa
    66481 tcttccggcg tatggatcgg accgtctacc atataaaagc tgcgctgacc agaatcataa
    66541 taggtcaatc cggtaaatcc taaatttttg gtcgattggt agattggctc cattatttct
    66601 tcattatcca tcaccttaaa gaaatgttct tcatcattga atagataagg aactgaaaaa
    66661 atcgaataat ctttagagaa actttccaac gctgaggcac ttacttttgt gaaatctact
    66721 gctcctgact gtgtcaattc gatcagttca cgttctccac ccagttgtcc atctgggaaa
    66781 tactggatct gtacggaacc atctgttttt tcttttacta aacgcccaaa ttcattcatt
    66841 gcatcaatta ctggctggct gttgcttgca taagcaaagc gcaaggtaac tatctcattt
    66901 acatctgcat ctgcgacagt tgccacacta gaattatttc cgcagccgct tagacctagc
    66961 agacctaggc taagcgaact tgctgcaaac agcagtgatg tttttttcat atgggtttcc
    67021 tttcttttaa caataagaga cgagcaattc tgtcaaacaa agcaccgtta acgactgtcc
    67081 ataaggcatt gctgtgattc cgatattccg ataaaaatct aaactgtccc ccattcctgt
    67141 cccgattgaa acgttttgaa cttctccttt ttcatcgatt tgttcaagca atccttggat
    67201 agcacgataa gcgacttctt tatattcttg tggtaaataa cgtttatgga ccgctttcaa
    67261 aataccgtaa gcaaatcctg cagttgcaga agattctacg taagaagaag gatcatccaa
    67321 caacgtatgc cataaaccgc tttcagactg ataagttttt aatgcacgca cttgtgcttc
    67381 aagtgtttcg atcaataatt tgcgcaatgc atcttctttc gataattcta agatttcaat
    67441 gatctctgga atagcaatcg tcaaccagca gtttccacga gcccaaaatg cttcggcata
    67501 gttatgattt ccttcaaacg tccagccgtg ataccacaat cctgttcgtt tatccatcaa
    67561 gtatttgata tggatcataa actggtaacg agcttcttct aaatattctg ggcgattcaa
    67621 cagtttcccg atttttgcca aaggaagcac tgtcatcatc aatgtatcgt cccacaactg
    67681 attcttattt tccggtccat atgtggcatg ctgtaacccg tcttcatttg tccgaggcat
    67741 gtcattcatg acccattctg cccattgttc taaataaggc aaatagcgtg aatctttcgt
    67801 gtcttcatac aagtaagcca tagtcaatag cggtgccatt gtattgacgt ttttgggcgg
    67861 agcgccttct tccatacgat cctcaaacca ttcattgacc acttgataag cgcgaggatt
    67921 ttttgtcatc tggtaatttt tataaatacc atatagaccg acaccttgtg gccaattcca
    67981 cactgtccag cttttgtcat ctactttcag tccatcaaag tccaacaaaa attcaccatt
    68041 atcgtctttg atcgttgtca ggttttcaac gagcaggtca attttttctt tcactaattc
    68101 tttaggtaca aaaatctctt ctttaataat catctttacg ccaccttttt aaacgctttc
    68161 attttatatc cttaatatag cagtaatacc gtccactata aatatcgctt tttgaacaaa
    68221 aactgtctaa aattgaccta aatgttaaat aacaaaagta actttcagtt acttggaaat
    68281 taaaaaaaga acttggagaa acaacgtttt ccaagttctt tttcatctat tttcttatct
    68341 tttttctacg ctttcttttg cttatccgtt gtaagcatca atgatgatct gtttcaattc
    68401 agagatcaat ggttctttcg gattagctgt cgtacattgg tcttcgtaag ctaattcagc
    68461 catacggtct acggttgtat ctagtgtttc ttgtgtgact ccttgagctt ttagactcat
    68521 gttgatgccg acagattttc ctaattcata aacagccgtt gctaaagctt cgactaattc
    68581 ctctgttgtt tcgcctttca gacctaagaa ttttgcgata tctgcgtaat ctgtatctgc
    68641 acggaaatag tcatatttag ggaacatcgc atgtttttgt ggatcttttg cattatagcg
    68701 gataatatgc ggtaaaagaa tcgcatttgt ccgtccatga gggatcccgt attccccgcc
    68761 gattttatgt gcgactgagt gacaaatacc taggaatgcg ttagcaaacg ccataccagc
    68821 cattgtagaa gcgttgtgca ttttttcacg tgattccata tcaggtgttt ttactgattt
    68881 ttctaaatag tcaaagacta acttgattgc ttgtaggctc aatccacgtg tataatctga
    68941 agccatgact gaaacatagg attcgattgc atgtgttaac acatccatcc cggtatctgc
    69001 tgtgacagat gctggaactg acatgacgaa ttgaggatcg acgattgcga catctggcgt
    69061 caatgcataa tctgctaacg gatatttcac atgtgtttca ctgtcagtga tgaccgcaaa
    69121 tggcgttact tcagaaccag tccctgatgt tgtcgggata catacgaatt gtgttttttc
    69181 tggtttgtcg atcttatatg tccgtttacg gatatccaag aatttttgtt ttgcaccaaa
    69241 gaaagatgtg tctgggtgtt catagaacat ccacattccc ttagctgcgt ccattgcaga
    69301 accgcctcca agagcgatta ctgtatctgg cttgaagtct gccatcagtt tcgttcctgc
    69361 gtatacggta tttgtcgatg ggttcggttc aacatctgag aagacttcga tcttcacatc
    69421 atttttacgt cgtgccaata cttcacgaac tgtatcgcag taaccgaatt ggaccatacc
    69481 agggtcacaa acgatcatga cacgttcaac gttttccatt ttctctaagt acaatagaga
    69541 gttcttttca aagaagattt ttggcggtaa tttgaaccat tgcatgttat ttctccgttt
    69601 cgctacagtt ttgacattga ttaagtttac agcagagacg ttacgagaaa cagagttctt
    69661 accataagaa ccgcatccca aagtcaatga cggaatcatt tcgttataga tatcaccgat
    69721 gccgccttct gcagatggtg tattcactaa aatacgacaa gctttcattc gaagaccgaa
    69781 tttgacatgc aactcttcgt tttctgaatg gataactgct gtatgtccta atcctccaag
    69841 attcaacata ccttcacaca aatcaaacgc atggtccgtg ttgttagctt tgatcatggc
    69901 aagaactgga gaaagttttt ctcttgatag cggataatct gggcctacgc cttccaattc
    69961 agcaatcagc attttcgttc cttttggtac actgattcct gccaattttg cgatttccat
    70021 cgcagaatgt ccaacgattg atggattgac cgcatatttt ccttcgttca tcactgcatc
    70081 ttctaatttt tgcaattcat ctggttttac gatataaact tggtgtgctt ggaattctgc
    70141 ttttactgct gcatagattt ctttatccac gatcacagct tgttcagaag cacaaatcat
    70201 tccattatca aatgttttcg aaacgatcaa atcgttcact gcacgtttaa tttttgctgt
    70261 tttttcaata taagctggta cgttacctgg acctacacct aaagctggtt ttcctgtaga
    70321 gtaagcagac ttgaccattc cagctccacc tgtcgctaag acgatcgcga tacctggatg
    70381 gttcattagc atagaagtcg cttcgatcga aggatgttcg atccattgga tacaattttc
    70441 aggagctccg gcagcgatag cagcatcacg taccactcgt gctgcttctg cagaagattt
    70501 ttgggcactt ggatggaaag caaaaacgat tggatttctt gttttcaacg cgatcattga
    70561 tttgaaaatc gttgtggaag tcggattcgt tgttggagtt actccgcaaa ccacaccgac
    70621 tggttcagca atttcaatca accctttttg gacatcttca ttgatcacac caactgtttt
    70681 atcatgtttg atattatgcc aaatatattc ggacgcatac atatttttga ttgctttatc
    70741 ttcgtaaaca ccgcggcctg tttcctctac agccatcttc gctaatggca tatgttgatc
    70801 caaagctgcc attgccattt catgcacgat atggtctact ttttcttgat caaagctttc
    70861 catttctttc aatgctgcat ttgctttttc tgccaaatca tcaatcattg cttgtacgtc
    70921 aattgtcttc gttttttctt tttctattac tttagccatc attatcctcc taatgggtca
    70981 tggttatttg ttaaataatt cacatgtata tcatacaata tttatttttc tatgtaaa
//
LOCUS       DACDRN010000007        70315 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDRN010000007 DACDRN010000000
VERSION     DACDRN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 70315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.007"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(122..499)
                     /locus_tag="IWJ80_000647"
     CDS             complement(122..499)
                     /locus_tag="IWJ80_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460409.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            755..1483
                     /locus_tag="IWJ80_000648"
     CDS             755..1483
                     /locus_tag="IWJ80_000648"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP9460410.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1483..1737
                     /gene="purS"
                     /locus_tag="IWJ80_000649"
     CDS             1483..1737
                     /gene="purS"
                     /locus_tag="IWJ80_000649"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP9460411.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1739..2410
                     /gene="purQ"
                     /locus_tag="IWJ80_000650"
     CDS             1739..2410
                     /gene="purQ"
                     /locus_tag="IWJ80_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP9460412.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2411..4633
                     /gene="purL"
                     /locus_tag="IWJ80_000651"
     CDS             2411..4633
                     /gene="purL"
                     /locus_tag="IWJ80_000651"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP9460413.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4618..6057
                     /locus_tag="IWJ80_000652"
     CDS             4618..6057
                     /locus_tag="IWJ80_000652"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP9460414.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6089..7132
                     /locus_tag="IWJ80_000653"
     CDS             6089..7132
                     /locus_tag="IWJ80_000653"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP9460415.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7129..7707
                     /gene="purN"
                     /locus_tag="IWJ80_000654"
     CDS             7129..7707
                     /gene="purN"
                     /locus_tag="IWJ80_000654"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP9460416.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7704..9239
                     /gene="purH"
                     /locus_tag="IWJ80_000655"
     CDS             7704..9239
                     /gene="purH"
                     /locus_tag="IWJ80_000655"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP9460417.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9255..10529
                     /gene="purD"
                     /locus_tag="IWJ80_000656"
     CDS             9255..10529
                     /gene="purD"
                     /locus_tag="IWJ80_000656"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP9460418.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10651..11565
                     /locus_tag="IWJ80_000657"
     CDS             10651..11565
                     /locus_tag="IWJ80_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP9460419.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11698..13263)
                     /locus_tag="IWJ80_000658"
     CDS             complement(11698..13263)
                     /locus_tag="IWJ80_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9460420.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13256..14707)
                     /locus_tag="IWJ80_000659"
     CDS             complement(13256..14707)
                     /locus_tag="IWJ80_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9460421.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14721..15737)
                     /locus_tag="IWJ80_000660"
     CDS             complement(14721..15737)
                     /locus_tag="IWJ80_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9460422.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15957..17045
                     /locus_tag="IWJ80_000661"
     CDS             15957..17045
                     /locus_tag="IWJ80_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9460423.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17099..18625
                     /locus_tag="IWJ80_000662"
     CDS             17099..18625
                     /locus_tag="IWJ80_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP9460424.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18627..19832
                     /locus_tag="IWJ80_000663"
     CDS             18627..19832
                     /locus_tag="IWJ80_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9460425.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20089..21066
                     /gene="guaC"
                     /locus_tag="IWJ80_000664"
     CDS             20089..21066
                     /gene="guaC"
                     /locus_tag="IWJ80_000664"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP9460426.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21330..22754
                     /locus_tag="IWJ80_000665"
     CDS             21330..22754
                     /locus_tag="IWJ80_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9460427.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23215..24324
                     /gene="pdhA"
                     /locus_tag="IWJ80_000666"
     CDS             23215..24324
                     /gene="pdhA"
                     /locus_tag="IWJ80_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP9460428.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24327..25304
                     /locus_tag="IWJ80_000667"
     CDS             24327..25304
                     /locus_tag="IWJ80_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP9460429.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25329..26972
                     /locus_tag="IWJ80_000668"
     CDS             25329..26972
                     /locus_tag="IWJ80_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP9460430.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26980..28386
                     /gene="lpdA"
                     /locus_tag="IWJ80_000669"
     CDS             26980..28386
                     /gene="lpdA"
                     /locus_tag="IWJ80_000669"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP9460431.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28548..28823
                     /locus_tag="IWJ80_000670"
     CDS             28548..28823
                     /locus_tag="IWJ80_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP9460432.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28829..29596
                     /locus_tag="IWJ80_000671"
     CDS             28829..29596
                     /locus_tag="IWJ80_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP9460433.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29764..31593
                     /gene="typA"
                     /locus_tag="IWJ80_000672"
     CDS             29764..31593
                     /gene="typA"
                     /locus_tag="IWJ80_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP9460434.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31854..33626
                     /gene="recQ"
                     /locus_tag="IWJ80_000673"
     CDS             31854..33626
                     /gene="recQ"
                     /locus_tag="IWJ80_000673"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP9460435.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLRKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33740..34042
                     /locus_tag="IWJ80_000674"
     CDS             33740..34042
                     /locus_tag="IWJ80_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP9460436.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34045..35208
                     /locus_tag="IWJ80_000675"
     CDS             34045..35208
                     /locus_tag="IWJ80_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9460437.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35234..38662
                     /locus_tag="IWJ80_000676"
     CDS             35234..38662
                     /locus_tag="IWJ80_000676"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP9460438.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVIGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38683..39807
                     /locus_tag="IWJ80_000677"
     CDS             38683..39807
                     /locus_tag="IWJ80_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460439.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39797..40234
                     /locus_tag="IWJ80_000678"
     CDS             39797..40234
                     /locus_tag="IWJ80_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9460440.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40218..40556
                     /locus_tag="IWJ80_000679"
     CDS             40218..40556
                     /locus_tag="IWJ80_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP9460441.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40675..41235
                     /gene="rsmD"
                     /locus_tag="IWJ80_000680"
     CDS             40675..41235
                     /gene="rsmD"
                     /locus_tag="IWJ80_000680"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP9460442.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41232..41723
                     /gene="coaD"
                     /locus_tag="IWJ80_000681"
     CDS             41232..41723
                     /gene="coaD"
                     /locus_tag="IWJ80_000681"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP9460443.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41713..42756
                     /locus_tag="IWJ80_000682"
     CDS             41713..42756
                     /locus_tag="IWJ80_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9460444.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42915..43643
                     /locus_tag="IWJ80_000683"
     CDS             42915..43643
                     /locus_tag="IWJ80_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP9460445.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43759..44004
                     /locus_tag="IWJ80_000684"
     CDS             43759..44004
                     /locus_tag="IWJ80_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9460446.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44001..44219
                     /locus_tag="IWJ80_000685"
     CDS             44001..44219
                     /locus_tag="IWJ80_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP9460447.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44355..44894
                     /locus_tag="IWJ80_000686"
     CDS             44355..44894
                     /locus_tag="IWJ80_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP9460448.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44887..47160
                     /locus_tag="IWJ80_000687"
     CDS             44887..47160
                     /locus_tag="IWJ80_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP9460449.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47198..48244
                     /gene="holA"
                     /locus_tag="IWJ80_000688"
     CDS             47198..48244
                     /gene="holA"
                     /locus_tag="IWJ80_000688"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP9460450.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48333..48587)
                     /locus_tag="IWJ80_000689"
     CDS             complement(48333..48587)
                     /locus_tag="IWJ80_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP9460451.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48987..49256)
                     /locus_tag="IWJ80_000690"
     CDS             complement(48987..49256)
                     /locus_tag="IWJ80_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460452.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49726..50181)
                     /locus_tag="IWJ80_000691"
     CDS             complement(49726..50181)
                     /locus_tag="IWJ80_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP9460453.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50319..52247)
                     /locus_tag="IWJ80_000692"
     CDS             complement(50319..52247)
                     /locus_tag="IWJ80_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9460454.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52444..53277)
                     /locus_tag="IWJ80_000693"
     CDS             complement(52444..53277)
                     /locus_tag="IWJ80_000693"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP9460455.1"
                     /translation="MFFANIIKAVILGIIEGITEWVPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53469..54173)
                     /locus_tag="IWJ80_000694"
     CDS             complement(53469..54173)
                     /locus_tag="IWJ80_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9460456.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54196..54822)
                     /locus_tag="IWJ80_000695"
     CDS             complement(54196..54822)
                     /locus_tag="IWJ80_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9460457.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFSVYPGHGDATTIEQEKRTNPFFNR"
     gene            55148..57523
                     /locus_tag="IWJ80_000696"
     CDS             55148..57523
                     /locus_tag="IWJ80_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP9460458.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57547..58038)
                     /locus_tag="IWJ80_000697"
     CDS             complement(57547..58038)
                     /locus_tag="IWJ80_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP9460459.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58225..59145
                     /locus_tag="IWJ80_000698"
     CDS             58225..59145
                     /locus_tag="IWJ80_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9460460.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            59299..59643
                     /locus_tag="IWJ80_000699"
     CDS             59299..59643
                     /locus_tag="IWJ80_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP9460461.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59833..60333)
                     /locus_tag="IWJ80_000700"
     CDS             complement(59833..60333)
                     /locus_tag="IWJ80_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9460462.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60861..60944
                     /locus_tag="IWJ80_000701"
     tRNA            60861..60944
                     /locus_tag="IWJ80_000701"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60895..60897,aa:Leu,seq:cag)
     gene            complement(61268..61906)
                     /locus_tag="IWJ80_000702"
     CDS             complement(61268..61906)
                     /locus_tag="IWJ80_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9460463.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            62241..62675
                     /locus_tag="IWJ80_000703"
     CDS             62241..62675
                     /locus_tag="IWJ80_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9460464.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            62698..63018
                     /locus_tag="IWJ80_000704"
     CDS             62698..63018
                     /locus_tag="IWJ80_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460465.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            63159..63350
                     /locus_tag="IWJ80_000705"
     CDS             63159..63350
                     /locus_tag="IWJ80_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460466.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(63421..63678)
                     /locus_tag="IWJ80_000706"
     CDS             complement(63421..63678)
                     /locus_tag="IWJ80_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP9460467.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            63836..64099
                     /locus_tag="IWJ80_000707"
     CDS             63836..64099
                     /locus_tag="IWJ80_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460468.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            64123..64311
                     /locus_tag="IWJ80_000708"
     CDS             64123..64311
                     /locus_tag="IWJ80_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460469.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(64394..64579)
                     /locus_tag="IWJ80_000709"
     CDS             complement(64394..64579)
                     /locus_tag="IWJ80_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460470.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            64794..65165
                     /locus_tag="IWJ80_000710"
     CDS             64794..65165
                     /locus_tag="IWJ80_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9460471.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(65315..65387)
                     /locus_tag="IWJ80_000711"
     tRNA            complement(65315..65387)
                     /locus_tag="IWJ80_000711"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(65352..65354),aa:Gln,seq:ctg)
     gene            complement(65433..65861)
                     /locus_tag="IWJ80_000712"
     CDS             complement(65433..65861)
                     /locus_tag="IWJ80_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460472.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(65921..66679)
                     /locus_tag="IWJ80_000713"
     CDS             complement(65921..66679)
                     /locus_tag="IWJ80_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP9460473.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            66974..67171
                     /locus_tag="IWJ80_000714"
     CDS             66974..67171
                     /locus_tag="IWJ80_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460474.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            67277..67759
                     /locus_tag="IWJ80_000715"
     CDS             67277..67759
                     /locus_tag="IWJ80_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460475.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(67817..68413)
                     /locus_tag="IWJ80_000716"
     CDS             complement(67817..68413)
                     /locus_tag="IWJ80_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9460476.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            68800..70266
                     /locus_tag="IWJ80_000717"
     CDS             68800..70266
                     /locus_tag="IWJ80_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP9460477.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT        23053 a        12025 c        14915 g        20322 t
ORIGIN      
        1 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
       61 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
      121 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
      181 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
      241 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
      301 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
      361 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
      421 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
      481 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattaaccaa atttttagtt
      541 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
      601 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
      661 gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat
      721 ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga
      781 aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt
      841 agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt
      901 gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca
      961 ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct
     1021 ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga
     1081 aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc agttggatga
     1141 tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg aagaaatcag
     1201 tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt
     1261 cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct
     1321 tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat
     1381 ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata
     1441 taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta
     1501 tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc
     1561 catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa
     1621 cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt
     1681 gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat
     1741 gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt
     1801 tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg
     1861 atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc
     1921 gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc
     1981 agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt
     2041 actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc
     2101 caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca
     2161 tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca
     2221 gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac
     2281 gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt
     2341 gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt
     2401 gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat
     2461 ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg
     2521 aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc
     2581 ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca
     2641 aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa
     2701 agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt
     2761 tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc
     2821 attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc
     2881 aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt
     2941 tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca
     3001 caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc
     3061 aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga
     3121 agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct
     3181 tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat
     3241 gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact
     3301 tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat
     3361 gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat
     3421 ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga
     3481 cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt
     3541 agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt
     3601 tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct
     3661 gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt
     3721 acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac
     3781 gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa
     3841 aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa
     3901 gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg
     3961 ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt
     4021 tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc
     4081 aatgattggt atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa
     4141 aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg atggcagtga
     4201 attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat
     4261 tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc
     4321 tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca
     4381 gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc
     4441 aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga
     4501 tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga
     4561 cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg
     4621 tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca
     4681 gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt
     4741 gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt
     4801 tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga
     4861 catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc
     4921 aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc
     4981 cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga tacggagata
     5041 ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct
     5101 ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc
     5161 gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac
     5221 gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc
     5281 cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag
     5341 gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac
     5401 atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa
     5461 tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc
     5521 ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt
     5581 gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta
     5641 tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt
     5701 cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc
     5761 catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa
     5821 acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt
     5881 gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa
     5941 gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta
     6001 tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag
     6061 cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga
     6121 tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg
     6181 tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca
     6241 attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc
     6301 aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga
     6361 tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa
     6421 tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa
     6481 tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta
     6541 tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac
     6601 gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta
     6661 ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc
     6721 agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc
     6781 cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg
     6841 gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg
     6901 ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat
     6961 ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcg aagaatcgaa
     7021 agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa
     7081 agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct
     7141 gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa
     7201 ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa
     7261 cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa
     7321 aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta
     7381 ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc
     7441 atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc
     7501 tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat
     7561 actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct
     7621 ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt
     7681 attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg
     7741 ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag
     7801 gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag
     7861 ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc
     7921 ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac
     7981 caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg
     8041 tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg
     8101 cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac
     8161 tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa
     8221 aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg
     8281 tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt
     8341 acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt
     8401 cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg
     8461 cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac
     8521 acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt
     8581 atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc
     8641 tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata
     8701 cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact
     8761 ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg
     8821 tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta
     8881 agccgactcc agcagaagca aaagcattag tgtttgcttg gagagctgtc aaacatgtga
     8941 aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga
     9001 tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg
     9061 cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac
     9121 atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata
     9181 tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga
     9241 gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa
     9301 gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg
     9361 ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa
     9421 tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt
     9481 taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag
     9541 cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa
     9601 tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag
     9661 aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg
     9721 tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat
     9781 ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc
     9841 ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc
     9901 gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac
     9961 cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt
    10021 tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta
    10081 cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag
    10141 tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc
    10201 gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg
    10261 ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc
    10321 tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac
    10381 gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca
    10441 ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag
    10501 ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac
    10561 aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt
    10621 tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat
    10681 cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta
    10741 aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat
    10801 ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga
    10861 gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct
    10921 aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg
    10981 gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagca
    11041 tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca
    11101 atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa
    11161 tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa
    11221 gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc
    11281 caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa
    11341 ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact
    11401 ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg
    11461 atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa
    11521 gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca
    11581 ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag
    11641 atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta
    11701 gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt
    11761 tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc
    11821 tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt
    11881 cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg
    11941 acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc
    12001 tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga
    12061 gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc
    12121 ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt
    12181 cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg
    12241 tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt
    12301 tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag
    12361 tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt
    12421 ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac
    12481 aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt
    12541 ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa
    12601 tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa
    12661 cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc
    12721 taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa
    12781 tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa
    12841 ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac
    12901 tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc
    12961 tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat
    13021 gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa
    13081 aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc
    13141 catctcacca tctccagctt cgccgacata agtgattgga taataagaac caaggacagt
    13201 tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata
    13261 catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc
    13321 cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga
    13381 gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg
    13441 ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa
    13501 ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat cctttgtgac
    13561 ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta
    13621 aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt
    13681 atcttatttt taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa
    13741 tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag
    13801 tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat
    13861 aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt
    13921 attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc
    13981 tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc
    14041 ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct
    14101 ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca
    14161 ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga
    14221 tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct
    14281 gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta ttgatcagct
    14341 cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt
    14401 ctttaagtgt atttgtatta tttttgctct ctcgaatatc attcctaagt tcttctagga
    14461 tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt
    14521 ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg
    14581 tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa
    14641 cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt
    14701 ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat attctgctgg
    14761 gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt
    14821 ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt acactcgact
    14881 acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt
    14941 acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg
    15001 tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc atttgtttct
    15061 aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga
    15121 acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga
    15181 ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca
    15241 gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat
    15301 aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg
    15361 ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct
    15421 tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact
    15481 ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt
    15541 cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt
    15601 ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct
    15661 tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa
    15721 aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga
    15781 taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa
    15841 atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg
    15901 ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga
    15961 aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa
    16021 gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc
    16081 cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa
    16141 aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac
    16201 aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt
    16261 cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg
    16321 atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag
    16381 taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac
    16441 cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca
    16501 acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg
    16561 gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa
    16621 ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc
    16681 catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg
    16741 aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga
    16801 tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata
    16861 cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa
    16921 cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata
    16981 aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa
    17041 attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat
    17101 ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt
    17161 agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg
    17221 agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga
    17281 cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga
    17341 aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga
    17401 gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga
    17461 aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt
    17521 atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc
    17581 cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa
    17641 tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca
    17701 taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac
    17761 gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg gaatggtggg
    17821 cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa
    17881 agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat
    17941 caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag
    18001 aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat
    18061 attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact
    18121 cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga
    18181 aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa
    18241 agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca
    18301 tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct
    18361 gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc
    18421 aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct
    18481 tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga
    18541 aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt
    18601 aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag
    18661 agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga
    18721 tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc
    18781 cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga
    18841 tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg
    18901 ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta
    18961 tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg
    19021 cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac
    19081 aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta
    19141 cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag
    19201 gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa
    19261 atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag
    19321 tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg attattttga
    19381 tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc
    19441 gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa
    19501 agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag
    19561 ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg
    19621 caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg
    19681 gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg
    19741 tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag
    19801 acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac
    19861 tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt
    19921 tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct
    19981 tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat
    20041 taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt
    20101 gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt
    20161 gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg
    20221 cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc
    20281 atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga
    20341 ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg
    20401 gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac
    20461 tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc
    20521 ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca
    20581 aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact
    20641 ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc
    20701 gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca
    20761 atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc
    20821 gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa
    20881 aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca
    20941 ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg
    21001 gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca
    21061 atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata
    21121 agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag
    21181 atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag
    21241 tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag
    21301 aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac
    21361 cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc
    21421 ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca
    21481 ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca
    21541 gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc
    21601 ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag
    21661 caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc
    21721 ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag
    21781 aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta
    21841 aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa
    21901 tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt
    21961 ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga
    22021 tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga
    22081 aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac
    22141 gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg
    22201 tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc
    22261 cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg
    22321 gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg
    22381 agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg
    22441 atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt
    22501 ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga
    22561 ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc
    22621 ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca
    22681 gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt
    22741 ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct tggatttaaa
    22801 tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact aagaaaaaac
    22861 cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa caaaactgta
    22921 taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa aatagaaaaa
    22981 atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat gctgttaata
    23041 tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt gtatttctca
    23101 taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt tattcactgt
    23161 ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg acaaatggca
    23221 aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc agatttccca
    23281 gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt acctgattta
    23341 tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt attagaccaa
    23401 cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa
    23461 gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct tcttccaggt
    23521 tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc ttttttatgg
    23581 tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct accaccacaa
    23641 attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt gaagaaacga
    23701 aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca aggtgatttt
    23761 tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta tatccaaaac
    23821 aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa
    23881 aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc gcttgcagta
    23941 tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc agtcctaatc
    24001 gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc aacccgctac
    24061 cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg cttccgtaaa
    24121 tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact
    24181 aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca aaaggtttca
    24241 gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt
    24301 tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa gcaatcacag
    24361 atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg
    24421 ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc ggagaagacc
    24481 gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt
    24541 tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc gaagcaatgg
    24601 acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact agaaatctac
    24661 ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg cattcggata
    24721 atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct tccaaccctt
    24781 atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt gttttcctag
    24841 aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct tacgaagttc
    24901 ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc acatacggag
    24961 caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat atatctgttg
    25021 aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt caatccgtgg
    25081 aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc gttgccgcgc
    25141 aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca atcggacgag
    25201 tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg
    25261 caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa
    25321 acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc gcagaaggtg
    25381 aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat acattactag
    25441 aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga acggttaaaa
    25501 atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta gaaatcgatg
    25561 caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc caaactccag
    25621 ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca agcgaaggag
    25681 tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa atcgtcaaat
    25741 ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa gttcaaaatg
    25801 acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac gtgattgttc
    25861 cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct cctggacaca
    25921 atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat
    25981 caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg gctatgccat
    26041 ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact gcaactggaa
    26101 aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg
    26161 caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct cctgcagcag
    26221 aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac ttggaagaac
    26281 gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac agtaagcata
    26341 cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg gataatcgga
    26401 aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg ccatatgtcg
    26461 tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct atcgacgatg
    26521 caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact gatacggatc
    26581 acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt gctattgcag
    26641 acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca gaagatatgc
    26701 gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg tttacaccag
    26761 tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa caaccaatcg
    26821 taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc
    26881 accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa cgtcttttag
    26941 ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg agattttgct
    27001 gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc tgccatccgc
    27061 gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg cggtgtttgt
    27121 ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca ttatcaagag
    27181 tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa
    27241 acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt tggtttccta
    27301 ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca
    27361 ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc aatcatcgca
    27421 acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt gttagattct
    27481 actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg tggcggtgtg
    27541 attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac gattcttgaa
    27601 ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt agaagatgat
    27661 ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac
    27721 ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt tactgctgat
    27781 tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt agaacaagcg
    27841 ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg tacgaacgta
    27901 cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca caaagccagt
    27961 tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt agattataaa
    28021 gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat gacgatcaaa
    28081 gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc agggaacggt
    28141 cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac gatcgaagat
    28201 aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta
    28261 gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat tcatcctcat
    28321 ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt accaatccat
    28381 atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta
    28441 gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg gtttattacc
    28501 acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg caagaatatc
    28561 agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg aagatgtggg
    28621 aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg aagacttatc
    28681 agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga cgggaatttg
    28741 aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca
    28801 aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata tcaagtcctg
    28861 gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa
    28921 atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt ttctaatcga
    28981 aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag ggtgtaatca
    29041 attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca ccttgaattt
    29101 tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg gcgttggaaa
    29161 gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa aaggaatagg
    29221 cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat cggaaggctt
    29281 acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag aaattgcgga
    29341 aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg caatcttgaa
    29401 aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag cgggaaatgt
    29461 cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt tgacatttga
    29521 aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg cagacctttt
    29581 gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa
    29641 agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgagaa aagtctgcta
    29701 taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa ggagttagtt
    29761 acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga
    29821 aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc acatacgcaa
    29881 ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta
    29941 gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga tactccagga
    30001 cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg
    30061 gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa aaaagcactg
    30121 gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc tgctcgtcct
    30181 gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga tgatgatcag
    30241 ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt gtcagatgat
    30301 ccagcagatc aagaaccaac catggcacct atttttgata caattattga aaaaatccct
    30361 gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact tgactacaac
    30421 gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa agtaggagac
    30481 caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac gaaattattt
    30541 ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga cttgattgct
    30601 gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat
    30661 gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt agttaataat
    30721 tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga agaacgtttg
    30781 atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc tccagatgca
    30841 tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa catgcgccgt
    30901 gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat cgacggtgta
    30961 aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat gggtagtgtt
    31021 atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac aggaaacgga
    31081 caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc aactgaattc
    31141 ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata tctgccaatg
    31201 cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa
    31261 gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt
    31321 acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga tttgactgtc
    31381 aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga ccaaacatct
    31441 gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt gaatgatgat
    31501 gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt agagaaaaat
    31561 gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag agagaaacca
    31621 gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt ggagaagtca
    31681 tctttgtcaa tggacataaa ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa
    31741 aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga aaggagatca
    31801 acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg
    31861 acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa gaaattatcc
    31921 agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt gggaaatcaa
    31981 tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg tcccctttaa
    32041 tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc gccactttta
    32101 tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc gatagacagc
    32161 agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt atccaagcaa
    32221 tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc tctcagtggg
    32281 ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa ctgccaaatc
    32341 gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct gacataaaaa
    32401 gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga gaaaatctac
    32461 gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat ttaaagatca
    32521 acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac cgtctctatc
    32581 atttgcttag aaaattcgat ttttccgtgg gtagatatca tggtggtcta aatgagaatg
    32641 agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt gtagcgacaa
    32701 atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat tatcaaattc
    32761 ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat ggtctttcca
    32821 gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt tttatccagc
    32881 agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa gcgatgacag
    32941 aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt ggcgagacaa
    33001 gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta gaagtaacga
    33061 cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa
    33121 tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta ggttttggac
    33181 atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg
    33241 aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct gttttgaaag
    33301 taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga aaggaaccca
    33361 agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg ctgcgggaac
    33421 tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt tcagattcca
    33481 cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt cagataaaag
    33541 gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg ataaaaaatg
    33601 cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa taaaaacaac
    33661 tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat taaggaatta
    33721 gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa
    33781 tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc tctgtatctc
    33841 acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt cccggcaagt
    33901 cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga tgttaagtga
    33961 gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt atgataaaaa
    34021 agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca tctattagac
    34081 tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat ggtatatagt
    34141 tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt cattaaccag
    34201 agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat gaaaacagat
    34261 gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat tttgctgctg
    34321 attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca aattgcagga
    34381 ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct gtctctgact
    34441 ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa acgtccactt
    34501 gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg taatgcggca
    34561 gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta tgtgtatact
    34621 ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct gatcaatatc
    34681 acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt tgctatttac
    34741 caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg ctactatgcc
    34801 atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa aaaaggattt
    34861 ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact gggagtgatt
    34921 atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt ccttgtaggt
    34981 atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc aatgtttttg
    35041 atccaagtat ttgtcaatct tggagggatt actggcgtta ttcctttgac agggatcacg
    35101 ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg tgtcggcttt
    35161 gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata aaaaaagatg
    35221 taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt
    35281 atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag aagaagatga
    35341 atattccgta caccgtttca aagcggatga agcatattta gtaggacaag gaaaaaaacc
    35401 gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat gcggagctga
    35461 cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga
    35521 agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt ttggagataa
    35581 aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag gcacagatgg
    35641 accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt atccgattat
    35701 gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg atgaaaagag
    35761 tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga
    35821 agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga ttctcgggga
    35881 tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc gccgtcacca
    35941 aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg
    36001 ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt
    36061 tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc aagtggaaca
    36121 tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt tgattgctca
    36181 aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta aattaaatgg
    36241 ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt taccagacac
    36301 agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg atgtcggaaa
    36361 tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa aagtatgtac
    36421 gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga aagaatttag
    36481 aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac atcctgaatt
    36541 ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat ttgaatttcc
    36601 tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg
    36661 ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga
    36721 tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga agggtgccga
    36781 tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata cgacatttag
    36841 agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca agcagattgc
    36901 acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg ggggagcaac
    36961 atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga gaaaaattcg
    37021 ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta
    37081 ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag cgcaaggaat
    37141 cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa aaagtatcca
    37201 agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg gcgatatcaa
    37261 cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa aggaactaga
    37321 acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta aaccgcaggc
    37381 agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc atctgcatac
    37441 tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag caggagtaga
    37501 catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta gcatgagcag
    37561 cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg aaaatgcaca
    37621 gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg aaaacggctt
    37681 gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt attcgaatct
    37741 acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca aacaaatgta
    37801 tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat ctaaagtaat
    37861 tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata tttatgaaaa
    37921 aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag aactagggca
    37981 acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca gacctgcatt
    38041 gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt
    38101 ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt tgatgtatcc
    38161 gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta cattactgga
    38221 cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg
    38281 cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg aagggaatcg
    38341 cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag ataattcgat
    38401 catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac aaattggagc
    38461 aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg
    38521 tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag cacgttttga
    38581 cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg gtgacctttt
    38641 gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag actgggtttg
    38701 ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt cttgtttccg
    38761 ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat gacaacgtgg
    38821 aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc gatcgatcag
    38881 ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg ttttgaaaca
    38941 agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca agacagcaga
    39001 gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg
    39061 atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac ttttgagtac
    39121 catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg tccgctaatt
    39181 gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa cggcctattt
    39241 tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca agtgtttggt
    39301 gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat caatcgcgtt
    39361 aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga acttcccata
    39421 tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga ttttttgaca
    39481 aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc gctaggcagt
    39541 gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt aatcagtaaa
    39601 caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc tactttttcg
    39661 tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc gggaacggat
    39721 tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga agaaaacgga
    39781 gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg atcaatgcct
    39841 ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg aacataaaca
    39901 gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga
    39961 agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg aaaaaaaacg
    40021 agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag ctttccgata
    40081 tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg ccgaagaagt
    40141 ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa aacactttgg
    40201 atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca aaacgacgcg
    40261 ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt
    40321 tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa gagacattgg
    40381 aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa cgttcttacc
    40441 gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta ttaaaagacg
    40501 aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat ctttaaaaat
    40561 ctcttatgag cagtttttaa ataaaaaata acttggataa cgatcgattt ttgatcattg
    40621 tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga gtagatgcgg
    40681 ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga
    40741 ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata ttttgatgga
    40801 gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc agtatctaga
    40861 ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat catcaaagaa
    40921 aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga tgcggacaaa
    40981 gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct tgatccgcca
    41041 tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg acttttttct
    41101 aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga aactgtggct
    41161 ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat ctatagaaaa
    41221 gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt acaaaaggtc
    41281 acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt ggtgttttta
    41341 tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg atcacaaaag
    41401 ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa
    41461 cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc aaagactttg
    41521 aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg gaaactgtct
    41581 tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa gaagtactgc
    41641 actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct ttagctggaa
    41701 agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat catatttgga
    41761 atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc accgggagcc
    41821 acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg ttctgggtct
    41881 ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa
    41941 atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc gacaaatgaa
    42001 gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc aatcgaacaa
    42061 gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata tgtcatgagt
    42121 gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt aacaaaagta
    42181 gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag ccaaaaagtt
    42241 ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc ttctggtgat
    42301 ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac tgaccatacc
    42361 gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg gccatcagcg
    42421 ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt gcgacatggt
    42481 aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg aatcggagga
    42541 atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt tgcaccagat
    42601 gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa
    42661 gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta
    42721 caagaagcgc ttgactatct agagaaaatg aaatgaaact aaataattaa agaggggttg
    42781 tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa
    42841 acaaaagaaa aaattgacgt aattagatgt tgaagggacg attttgccag agctcactgt
    42901 ttcttcaatt ttaaatgaaa ggagtaatga atgattttta ctggtcattc tttgaaaaaa
    42961 gaacaatgat agaaaaaatc agcaagaaaa tactagcttc acccaaattg atcatttgga
    43021 tgtctatgat agttcttatc ttttgtatcg caagtggttt tttattatca aatttgctat
    43081 tttatagaga aacagaagag actgctgcaa aagaaatact ttcttcgttt agttccagtg
    43141 gagaagatcc atcagctgaa ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt
    43201 acaccgatat caaaggaagt gtgaaggagc ctggtatcta ttccttttct tcagaagaac
    43261 gggtctatga tgtactaaag cgtgcaggtg gattattaga agaagcggat agcgatcgaa
    43321 tcaacttttc tgcgaagata gaagatcagc aagtcctata tatccctgct gtaggtgaag
    43381 agctgccaga acatcttgat cagttggcat cctcggaagg aaaacagcct acagcagatt
    43441 ccgagcctcc caaaataaat atcaatacag ccactccatc agaacttcag cagattcctg
    43501 ggatcggatc agtcaaagcg caagaaatca ttcgatttag agaagaaaac ggatccttcc
    43561 agaaagtgga agacctgcag gagatttctg gtatcggtga aaagacagtt gaaaaactaa
    43621 aaaacttcgt tacaattaaa tagaatgatt caaaatcgac tggcacgagc aataaatgga
    43681 cgctaatgga tactaagaat aatccaattc taagagcttg gcaggacgag gaggtagtat
    43741 ttttggatag gaaatagctt gattgttcgc aaagaaacat taaaaaaacc aatgctgaat
    43801 gtctatctcc aaaataaaat atctgggatt catattatga atacggctgt cagcggcaat
    43861 aacagtcaag ctctacgaga acgctttgcc aaagatgtac ttagctatac agcagataaa
    43921 gtatttattc tcattgggac gaatgatcta gcagagcata aacaactttc taaagaaacg
    43981 taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa agactatagt tctgatcatg
    44041 ctttctttat cacgccacca gcagtcgatg agacaaaaca aaaatatcgg aataacaagt
    44101 tgatcaaaga atatatctct gttctcacag aagtagcaaa acgccatgat tgtgaagtat
    44161 cgatttttat acggaatttc ttgaacaaat ggattcgaag aatttttata atgggctaat
    44221 ggatgacgga cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg
    44281 ttatatttaa agaatatttt gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg
    44341 gaggaatata aaacatgtta gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag
    44401 ttactgataa gtcacataat gaacgcattc cttgggacca gtactttatg gctcaggcag
    44461 tactgttatc tttacgtagt acgtgtacac gtttagaagt aggagcgaca ctcgttcgag
    44521 aaaaaaggat cattgctggc ggctataatg gagcagtcag tggagatgtc cactgcatag
    44581 atgaagggtg ttacgtcgtg gatggacact gcttgcgaac gatccatgca gagatgaatg
    44641 cactcctaca gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc tatgtcacac
    44701 attttccatg tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt
    44761 atctccatga ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat
    44821 ctgtacacca agttgcttta gactcacagc atttttcaaa acttcaaaat gaattaacag
    44881 gaaaacatgt ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt tccggcaatt
    44941 acgtcgatga ttatctgttt gttgtattac actgacaatt ggggttatcg tttgcttctt
    45001 gccggaattt ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt
    45061 ttgtgtagca taacggtatc catttcttat aggctacaag agtattcatt tttagaggaa
    45121 aaaacaacat ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa tggtaatttt
    45181 ttgacaatgg aaggaaggtt atcagagaat aaacaaaagc tccagctttc ttatcaagta
    45241 aacacagaag cagaattgaa aacatggctt aacttccgag gaaagggaat agaattgact
    45301 gctgaaggcg agttcatctc tccagcaaaa aagcggaatc tttataattt tgaccaagaa
    45361 gcttacttca ggaaccatag aatttctgga cggttccaaa ttcaaaagat tgaacagatc
    45421 accatgcaaa atcactggcg aaactggctt cagagaaaaa gaggatcttt tatctccttt
    45481 gtccaatctg aatttccgca aaaaacaagt gtatatatta attctttatt atggggctat
    45541 aaagatgcag gattcagaga accagaagaa atttttaggg agagtggttt gctgcatctg
    45601 ttcagtctat cgggtctaca catccaattt tatttagggt ggctgtatta tttatttcga
    45661 cgtatcggat ggccactaca cgtcagtatt attccgttga gtctactaac tatctgttat
    45721 gtttctattg caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga
    45781 ttgctagtca aattgctgaa taagcattat tccaccatgg acttatttgc agttgtcttt
    45841 tggcttcttt tactatttga tcctcgattt ctttttcatg cgggtggaca gcttagtttt
    45901 ttcatgagtt ttttgtttat cctgatttct tttgatggag attggttgaa aaaaacggcc
    45961 tctcacgtcc tgtttactat gatcacgatg ccgttgattg tatggcattt ttatgagtgg
    46021 cctattttag gtagtctttt tacattactg attgcaccgg tattcaagtt tctttttctt
    46081 cctggtgtgt tatttctcac actcttcaat cgctggctgc cggaaacttt acttttgctg
    46141 atagaagagg gattaatctt atttgagcga ataatcgatt tttcttcagg gtcgtctctc
    46201 attataggac gtttgcccct tgtgctatct gggatgtata ttttcggatt actgatttgt
    46261 atggataggt tgaaagaaca acctgtgaaa cacttgtttt atgttggtct tttttctttt
    46321 atttttttga ctttgccatt tttacgcttt tcttcaacga tcgcttttat cgatgtcggt
    46381 caaggagaca gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt gatcgatact
    46441 ggaggtcagt tacactttga aagagaaaag tgggcgcaag gtatgatacg tcctcctgct
    46501 gaaagaaata tcgttccata tctaaaaggt caggggataa gtgtaatcga taaactcatc
    46561 ttgacacacg acgatacgga tcatgtggga gaattaccca cgttatctca gcattttact
    46621 atcaaaaaaa tttatatcgg ttggggcgct ggacgaaatg agccattaca aaggcattta
    46681 tctgaagctc aaaaaaatgg tactgagatc attgaaatca aacatgggga caggatcagc
    46741 ggttattatg atctttatgt attgactcct tttgaaaagg gagagggaag aaatgaagat
    46801 tcgattggtc tatggatgga atataacaat aggcgctttt tatttttagg agatttgaat
    46861 caagcaatgg aaaaacaatt gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa
    46921 ttaggtcatc acggtagccg tacgtcaagt aacacagatt ttctttccca tattgaagca
    46981 aaacatggga ttatatcctg cggcgtaaat aatcgttatg gtcacccgca cagcgaagta
    47041 ttggagacac tagaagaaaa tcagataaaa acatggcgaa cggatcaaca aggaatgatc
    47101 acctatcgct ggcatccagt ttttcatcct catgggctag tggaaacaat gatagactag
    47161 taattctcat ctgctcatgg taggataatt tttgaaagtg gtgagtaatt tgagtataca
    47221 agcagagtta caaaaaatac gtaaagaaaa acttgcatct tgttacttga ttttaggaac
    47281 agaaaagttt ctccaagatc aagttcggac agaaattgtc aaaaaaatcc aattagaggg
    47341 cgaagatgac ttaaattttt taagttttga catggaaaat gcaagtttag atgaggtagt
    47401 agctgaagca gagacgttgc cgtttttcgg agatcaacgg ttagtattcg tagagaatcc
    47461 ttatttttta acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg atttattgac
    47521 ggcttatttg aaagaaccat tagaatcgac tgtattagta ttctttgcgc catatgaaaa
    47581 gttggatgaa cggaaaaaag taacgaaaca attgaaaaaa acagcaatta tcatcaatgt
    47641 ccaacagttg aatgaaaaag aagtacgtca atatctgatg aataccttag agaattcctc
    47701 aatcaaaatg gaacgaagtg caattgattt attcttgagg ttgaccgact tagatttatc
    47761 caaaatgatg agagagttac agaaattggt cctttatgga caaaatcaac agcaaatcac
    47821 ggtaagagaa gtagaagaat tagttcctaa aacgttagaa cataatattt ttgacatgac
    47881 ccaatatatt ttaaaaggga agacggaaca agctttgcgt ttatacgaag atttagttat
    47941 gcaaggggaa gaaacaatca aaattaatgc gattttgctt tctcaattac gtttattttt
    48001 acagacaaaa tttcttgtaa agattggtta tcagcaggca aatattgctg aaacattgaa
    48061 aatccatcca tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg atgaacattt
    48121 gctggtacag ttgtttgatc aattagttga gatggattat caaatcaaaa cgggacaaat
    48181 cgaaaaagaa ttaagttttc aactattcgt tttgcgcaca gggcagttgt taagaaatcg
    48241 ataagaagaa agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg
    48301 acaaataaaa aaatcggccg aagccgattc ttttatttag cgattttttt gcttaaacga
    48361 gatttgtcgc ggttagcttt gtttttgtgg attagacctt ttgattcagc catatcaata
    48421 gctttaacag cttctttata taacgcatct acgttgtcag cacctgaagc tacagcatct
    48481 tcaaattttt tgattgcagt acgcatagcg cttgtttgag atgaattttt cacattagcg
    48541 ttttcgcttg tacgaacacg tttgattgca gattcaatgt ttggcattag tttcacctcc
    48601 gataacttaa gttcttacgc gtgttaccgc ataattcaac attcattatt atacctaaag
    48661 gcagcgtgct ttgcaatatg aacatgacaa gtttttttcc ttaacagtct tttattagga
    48721 ttttttttca gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg
    48781 gaggcaatag cgtcaaacca aactagattt ttatttatct atttaaacaa agtttgaatg
    48841 ggaatgtttg gtactaagtt attatataac aaagagaacc gtttgcaaag ttttttttga
    48901 aaagggatac gattccctgt tgttctttgt ttttctatta agaaactgca gttattttat
    48961 aaccagtatc ctttgatcag taaaggctat tctggtgtac cgattatttt aatgtattca
    49021 tcgatatttt ttatcaacat tttttttatg accaactggt tccgacggtt ccatttgcca
    49081 cttacttcca gcacatattt atttaggggc aaacgtaaga tatattttgc caaatagcga
    49141 tctttaagca ggccattgat cacacggtcc tcaagctgca agttgatccc agtcagcatc
    49201 attggctgtt cttgtaaaac gtacgtatca attaaaagac cttcaaaagt ttccacaagc
    49261 gtcttccttt cttttcaaag taacctgaaa acaggcacta attcttccat tttgatattc
    49321 gttgaagtgg atgcgatcat tctttctttt gtccactgta atctagtata acaaaaaaga
    49381 gcagaagagc caaaaagaaa aaagagcatc gaattctata ggaagtctca ttggcacggc
    49441 ctaaattatt acaaaacatt gattaaaaaa agcccctgca tcgcaggagc atcccctata
    49501 tcgttttaat aggtcaatca aaaagcagta tcaaacacat tttgtatcaa atcaggaaat
    49561 aatagataag ctaagcccag aatcaaaagt accgctagaa gaaaccagcc gattgttcct
    49621 atgaaatact ttaatttttc gaacattgta tcacctcgtt gtcagtatat cacggatcag
    49681 taaattgggg gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc
    49741 aaatacttgt aagatacagt aagtgatacc agaagcaatg accggtccgg cggcgattcc
    49801 tcttaagaag acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc
    49861 agaagcaatc aatcctacac ccttggaaga taagacagcc actagcccac cgcaaaaaac
    49921 agctacccag ccaatagggg atttaaaggc ttctaataga tcacgtagac cgatttctcc
    49981 tgttgcgata gggattagaa tagccgctgt gatgatcgtc accccaatat tgattccttt
    50041 gccttgaagc caagggaaaa aggaacggga ggctggtatg agcttcataa tcaaaacaaa
    50101 agctgccgct atcatcagac tttgattttt agctaaaatt gctacaagta aaataattcc
    50161 taaaaaaagc caactttcca tgaaatatat tcctcacttt cttcgaaaca atcataacat
    50221 gtttttagaa agttacaaaa gtaggaattc atccttcaag acctattcat aagctgtttt
    50281 tttcataaaa taaacaaaat cataaaaaaa cactttgctt atttgacaga agacagccgc
    50341 gctttgtata gtggacaaga cttacatttt tttgaagaat cagaaagatc aggtaaaggt
    50401 cttagtaaac gcaagccatt cagaataacc aatattgtgc ttccttcgtg tcctacaact
    50461 ccaagaggta gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag aataacaaga
    50521 atcgaaaaaa taatattttg tttgatgatc cgccgcattt ttaaagatag ccgatgactg
    50581 tagacaaatt tatttaagtc gcttttcatt aagacgatgt ctgcgatatc catagcggca
    50641 tctgtccctt gccccatagc gacacctaaa gaagcggctg ctaaagcagg cgcatcattg
    50701 actccatcac caaccatcgc atttacttgg taaagttcat tctctttttt gactagttgt
    50761 gttttttctt ccggagtaca gcttgcatag taatgttcaa tccctacttg ttcagcaatg
    50821 gcctttgctg tgccagaatg gtcgccggtt atcatacttg tatggatgtt ttgggattta
    50881 aagtaggaaa ttacttgttg ggtatttgct tttggaatat ctagcagacc taaaacagca
    50941 attaactgct gatctttaga aagataaatc acggttttgc cttgattttc caaacgttcg
    51001 atcatttccg caatctcttt gctgattatc atcgtctcag gatcatatgc gtgcttgcca
    51061 actttccatt gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat ttcttctatt
    51121 ggtaagtatt ggatttcttc aggtattgtg caatcgaaat attgcaaaat cgcatgagca
    51181 agtggatgcg tcgttttttg ttccatagca acaagaagtt gttgtgccaa atccttttca
    51241 tcaaagaaaa agctgtcagt tacaactggt tttcctttag tgagcgttcc tgttttatca
    51301 aaggcaatcg cttttaattc agaaagcttt tctaaatgga taccgccttt gattaaaatc
    51361 ccattttttg cagaatttga aagagcagct agagaagctg gtgtagcaga tgcgacaagc
    51421 gcacaaggag aagccacaac taataatacc attcctcgat aaaagctttc atcccaactc
    51481 caacctagga aaaagtgggg gagaaggatc attagaggaa cagcaagcaa aatcaatttt
    51541 acataattat tttcgattcg ttcgataaat gaagctgttt gagttggcgt attctgcgct
    51601 tcttctacta attggataat cttagcaaag acagtttcgg aactcgtttt tgttactcga
    51661 atcgtgatgg gatttccttg attcagcgtt cctgcataca tctcatcgcc taaatatttt
    51721 tctacaggca cagattcacc gcttaatgca gactcatcga tcgttgcctg tgtgctttct
    51781 aaaaagccat ctatcggaac agctgctcct ttagcgacaa ataccaagtt atccaccttt
    51841 agttgagaaa catctacttc ttgcatttgt ccattttctt ttaataattg ggctttttgt
    51901 ggttgaagat tcatcaaagc agtaatttct ttttgacttt tattagttgt gtattcctct
    51961 aatgcaccgc ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta attaccgatc
    52021 aaacaggcac caatagcagc taaagccatc aataaatcca cattcagtgt ccgatcattc
    52081 cacaattctt tcaagccttc cgatgtttgt ttatatccac cgatgataat ggcactgata
    52141 taaaaaagtg gggtgatggt aagagagaat cggtcgaaga taaaaccgaa gatcataaat
    52201 aaaaggcagc cgattgttgc tgcaaaagct tttttctgat tagtcatcgt tattagacct
    52261 ccttgtcaaa acaaggatag cataataaaa atgtaattga taataataac agctaaatga
    52321 gaacgataat ggttatcatt tggtgattaa gcttgatgtt gtttttatta tttataatga
    52381 ttattattaa agaaaataaa aaaccagaac atttctcaaa taagagaagt gttctggttt
    52441 tttctacatt gcaataaacc aataaccaat caaaatgata ccaaggataa tacgatacca
    52501 gccaaaagcg gtaaagtcgt ttttcttcaa atagcttagt aagaatttga tagctaagac
    52561 ggaaaccaca aaagatacga atgttcctac aagaagaaca acagtttctt ccattttgaa
    52621 tgaattccct tcagctaaat atttgacgat cttcaagccg cttgcgccaa acatgacagg
    52681 aatccctaag aaaaatgaaa actctgtagc gacaaaacgc gaagcaccaa tcaagataga
    52741 tcctaggatc gttgcgccag aacgggatgt tccgggaaca agtgccaaga cttggaataa
    52801 cccaacaaga agagctgctt ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt
    52861 gtttttgttg cgtttttcga caataataaa ggcaataccg taaacgatca aaacaatggc
    52921 aactggtaag aacttgtgga aatttgcttc taaccaatca tccaaaggca aaccaatgat
    52981 gatggctggt atgcaagcaa cgatcacttt aaaccaaagt gtccacgtat cttttttttc
    53041 taccgttgat ttacttggag aaaatgggtt taacttgtga aaataaagaa cgacgacagc
    53101 caagatcgcc cctaattgga taacgacatt gaacatcgac ataaattgtg aactggcatc
    53161 cagtttgatg aattcatctg ctaggatcaa atgaccagta ctactgatgg gcacccattc
    53221 cgttattcct tcaataatac cgagaatcac tgctttgata atatttgcaa aaaacataaa
    53281 ttccttcctt cccaattaag atacatctta tagtataccg ttattttttg agaaacccaa
    53341 tctgtttttt aatattaaga aaaacttgag aaataaagca gataagtaca cgaagagaaa
    53401 aaagaagaca aagccctcgt tcagactttg ccttttatga ttctctggta aaaattaaaa
    53461 tatccccatt atagaaacga gtcgcgacta tgtgttttcc cgttatcgat ataagacgta
    53521 tcattgatgc cgtggataga aaatcgaccg tttgcataag tgacctttgt tacgctgcca
    53581 tttaaaagcc cagcacgaac aggctgttta ggatcgatca gattaagcaa aaaggcgatc
    53641 gtcaaaccat gagatacgac taaaacattt ccaccaccat ttttttcgca gtgatgggcg
    53701 atatcttcaa atcctgtcca tacacggtct cgcaatgttt cataagtttc cgcccaccca
    53761 gcggtatcta agttgtatac agcattagca atttgttcaa aagaaacttc tgtggtaaac
    53821 atttcatcat atgttttaaa attcaataca cgagggatca caccccacat ttccatgtca
    53881 tatccacctt caagagaccc gaaacaccat tctctaatcc ttttttcaat aaaataagga
    53941 atttcttttc ccttagaatg ttctcctaga ataatacggg cagtttcgat tgcacgacca
    54001 ctgtcactag aataagcaaa tgaaaagtcg acatttttta acccgcgacc taaataatga
    54061 atgccttctt gtccttgctt tgtgaggggt gtatcacacc agccttgtac tcgttcaatt
    54121 gtattgaaca tcgtttttcc gtgtcgtacg atataaagtg tagtttctgt cattttattc
    54181 tcctcctaag tcgttttaac gattgaaaaa aggatttgtc cttttttcct gttcaatcgt
    54241 cgttgcatcg ccatgtcctg gatagactga aaattcatca ggtaatacaa acagctgggt
    54301 ccgaatacta tgaagtaatt gttctaaatt ccctgtatac agatcggttc taccgatact
    54361 tcctctaaac agggcgtcgc cagaaacgac aaaatcatca aaaataaagc tgacgcttcc
    54421 tatagaatgc cctggggtag ggacgacgcg aaaagacatg tttcctaaag tataagtttt
    54481 catttcaaat tcatattctg caggcgagac aataacatca gcaatatcat catgtctacc
    54541 gagtccagaa agattcaaaa tcggatcacc tagccatttt tgctctagtg ggctgacata
    54601 aactgggatt tgataatacg cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt
    54661 atgggtaagc aaaatcgcta ccggctgttg gtttgttttt ttgatttctt gtttgattcg
    54721 ttccgcatct tctccagggt caatgatcaa aagtgcttct tcgttgtaga ccaaataaca
    54781 gttttcttca atcattcctg tttttaatct ttcgatatgc atgtgcattc ctccttgata
    54841 gccaaaactt ctttcatcca ttagtataac gcattcattg ctaggaaaca aaaacaggta
    54901 cttatttctc tttacttatc tgaattatca gactatatga aaagtatttg gatattctaa
    54961 agagaaggaa agtaagtttt catagcattt taatatgtcc ttgttacatt agattcatcc
    55021 aaaaatccaa attctatatc ctatatccct gcctaatcag cagggtgttt ttttatgttt
    55081 ggaaagagag ctctttttgc taaactaggt tttttcaggt aatcttagag agaaagaggt
    55141 ggtggaaatg atcaaacagt caaaaatatc tgtgcggcac ttagtagaat ttattttgcg
    55201 acgtggaagt atcgacaatc gtaaaaaaag taaccatact gcattagaag gagcgagaat
    55261 ccaccgcaag ctccaaaaag atgcgggaga aaactatgaa aaagaagtct ttttaaaaac
    55321 aacagttcaa ttagatgaat attacctgat cgttgaaggc cgtgcagacg gtattttcaa
    55381 aagagacggt atttattata tagatgaaat caaaacctcc gaaccacgat ttgaagattt
    55441 agaaccagaa caagtggaac ttttctttca tcaagcaagg gtgtatgcct atatctatag
    55501 tcaagaacat gatttacaag aaataaatct gcaattgact tacttccaga cggcagaaga
    55561 agtgatcacg agaaaggttg aacatcagac aagagagcag ttagaaactt tttttaaaaa
    55621 gctgactgaa gattataaag aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa
    55681 tgcctctttg atggctttga aattccccca tgatgagtat aggaaaggtc agcgtgaact
    55741 agcggtagct gcttataaaa cgatccgtac gaaacagaaa ttgtttgtag aagcacctac
    55801 cggaacagga aaaacaatct ctacgttatt ccccgctttg aaagctgtag gggaagaaga
    55861 gggagaaaag atcttttatc taacggctaa aacaataact agacaagtag ctgaagatgc
    55921 tatgacagca ttaaaagata caggagctga agtaaaaagt gtcactttga cggcgaaaga
    55981 caagatctgc tttttagatg agacgacctg taatccagat cagtgcccat atgccaatgg
    56041 ttattacaat cggattaatg aaggtttatg ggatcttttg aatcatgaga atcagatcac
    56101 tcgtgaagtc atcgaaactt atgcaagaaa gcatacactt tgtcctttcg aactatcatt
    56161 agatgtcagt atctggtgtg acgtaatcat tggggattac aattatttat ttgatccaac
    56221 tgtttattta agacgttttt ttgaagatga aaaaaacgag gactatctgt ttttgattga
    56281 cgaagcacat aatttggtga atcgttcccg agagatgtat tcggcagaac tatcttatga
    56341 aaaaacgaaa cgaagtaagg aagctatacc aaaggaattc aaaaaacttc atcgccgttt
    56401 caacaaacta ttaaaagaat ttgattcgat ccgagaaata gcaaaagaag atcattggga
    56461 ttatcatcat caaaaagcac cggctgaatc tttagtaaag gcaggttatc aattaagcga
    56521 aaaaattaaa gaatggctgg cagaatttcc tgagcaccca tcacaagaac aattgcttcc
    56581 ttattatttt gatttgcttc attttttgaa ggtcagtgaa ttttatgatg atcactacga
    56641 aacaacagtc gaaaaaacgt atcgcgatct gatagtaaaa gagttttgta ttgatccaag
    56701 cttgtttctt gagcagtcgt tggataaagg aaaaagcagt attctttttt ctgctagttt
    56761 ttcaccactc tcttattatc aggaaacact ggggggacaa aaaagtctgg cttacaaact
    56821 gccgagtcct ttcccagaag gaaaccagca agttttcatc gcaaattacc tagagacgac
    56881 ttatcgaaaa agggaacaca gtctggcaaa attagtagag acgattcatg cttttgctga
    56941 tggaaaggtc gggaattatt tcgttttctt tccttcttat caatatttag atcttgcggt
    57001 ggaagcattc agacagcaat atcccgacag caatgtgcta atccaagaaa cggacatgaa
    57061 tgaggaagaa cgcgaacgat ttttaacaaa atttcaaaca aatcccgctg aaacattgat
    57121 tggattttgc gtacttggcg ggattttttc agaaggaatc gatttgagag gggaccgatt
    57181 gatcggcagt atgatcgtag gtgtaggttt gccgcagatg aatcatgagc aggaactgat
    57241 caaatcatat tatgatgaga aggaacacct tggatttgct tacgcttatc agcttcctgg
    57301 aatgaacaaa gtcttgcaag ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat
    57361 cgttgtattg gcagatcaac gattttctag tttttcttat cgacgtttat tcccacaaca
    57421 ttggcaaaca gcaagagaag tacgaagtgt tggggaattg gaagaaggaa tacagcgttt
    57481 ttggttatca aaagaagcag taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt
    57541 ttttgattaa cgatagttag tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa
    57601 agcaatcaca gcttgtagat catcacggtt cttacctgtc actctaatct gatcttcttg
    57661 gatttgtgct tttactttca gtttttcatt tttgatagct gtagtgattt tcttcgcgtt
    57721 ttcacgatcg attccgttga ccaaatcggc agtttgtcga gctttgccac ctagagcgtg
    57781 ttctgattcg gaaaaatgga tatttttgat tggcacattt cttttgatca gcttaccaaa
    57841 taatacatct ttgatctgtt cgattttgaa atcatcatcg ctcaatacaa ctagattgct
    57901 gttctctaat ttgatttcta ctgtagaatc cttgaagtca aagcgattgg acaattcttt
    57961 tgttgtttgc tgaatcgcat tttttacttc ttctgtattg acttctgata caatgtcgaa
    58021 gctagcatct tttgccatag gaaccctcct gatatacata taaatattta ccatacatct
    58081 atcttatcat aggattaggg cagattctta cctgtttgct aaaattcttc tcatttgagt
    58141 tcgaagtatt ttattttaat agataaactg ttattcttac atttggaata ccaaagtagg
    58201 ggagaggtgc ttgtgagtaa aattatgact gcgtatcata aaaatgaaag attaaagaaa
    58261 atagtggtta ttttgataac cggattgacc gctgcggtcg ctttaaatta ttttttgatt
    58321 ccagccaatg tcttttctgc agggatgaat gggatcgctc aaatcatttc ctcgctttta
    58381 tatgaatggt ttcatatcaa gatagacaca ggaacattta tttttctttt gaatatccct
    58441 gtgtttgtat tagggtttct caaagtagga aaacgagcaa cgatccttag ttttgtgaat
    58501 gttgtatgtg tatcggtgat gacgatgctg cttccagtcg gacagatcac tgataatatt
    58561 ttgatgaatg ccctagtagg cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa
    58621 atgggcttta caactggtgg gatggatatc atctctcttg tactttcaca aacaactgga
    58681 aaaacagtcg gaaactacat gctgattcta aatggtatca ttgtagtcat cgcaggcttc
    58741 ttctttaact gggaaagtgc cttgtatacg attatttcca tttatgcaat gactcaagta
    58801 attgatgcca tccacacgag tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca
    58861 gaagaggtaa cagaagaact agcaaaacgg atggtacgtg gaatgacatt actgccgtct
    58921 gtcggtggct attcaaaaaa acaagggaac atgatcatga tggtcatcac ccgctacgaa
    58981 atgtatgatc ttgatcagat cgttcatgaa atcgacgacg atgcatttat caatatttta
    59041 ccaactcatt cagtatttgg ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca
    59101 actggcgtat tgccagaaat aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt
    59161 ttagaataaa tttaatctat tacatctaat aagaacattt ttataaattt aaacgagaat
    59221 cttacttcta aattctcatt tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt
    59281 gttaaggagt gtcgtacgat gagcgaagca aaccaagaac ggtctggaca agaaatagaa
    59341 ctaatcaaag aacgtattct tgctgccttg gaaatggtga ttgatcctga attaggaatc
    59401 gacatcgtca atttaggctt gatttatgat atagaattca atcctgaaaa tggagaaaca
    59461 gtcattaaga tgactctaac aactatgggc tgtccattag cagatatttt aacagaatct
    59521 atccatgacg cattaaaaga agtgccagaa gtaacgaaag cagaagtcaa actagtctgg
    59581 tatccagcat ggacgacaga taagatgagc cgttatgcac ggattgcgtt ggggatacga
    59641 tagaaatctt ggctgactag ctatgagaag caaataggaa aaacaaaaaa gccttttatc
    59701 ctttctagaa gaaaagagat aaaaggtttc tttgtttata aaattttgtc tgctatatta
    59761 taagttaatt aaataaatgt gttctattga taaaggacat tcaaatccct ttccttttgg
    59821 aatccttctt ttttacatca gtttgatttt cgtatagtat tctttctcat cttcacgata
    59881 aatcgcagcc ccattttttt gttgtgcttt aagactgcca agattttttt tatttacaga
    59941 caaaagtgcc tcatcaatgc cgattttttt tgcctcttgt agtactaatg ctaatccttg
    60001 tgtggcatat ccttttttac gataattagg ggagatgccg taaccaatat ggccagcgcc
    60061 gtttcttaat gtttcgttca gtttatggcg caaattaaaa attcctacat actcatgttc
    60121 atcactttct aaaataaaga cggttgcggg aacgtaactc tcaggaagat cgattcctaa
    60181 agacgttttt tcgcattttt ttacataatc taaaaattca gcaaatgtat agccataagc
    60241 attgttgata aatccactct catttggtcc aaagctagtt tgtagctgat aagctttctt
    60301 taaatctgct tcccatagtt tgatcagttt catcgtattc acctctttaa gtaaccttat
    60361 tcatttgttt tctactatag cactattgtt gtaaatagcc atgcctattt ttatttattg
    60421 tgtcatatcg tattaagaat catcgaacaa tacaaatatg aattacaata aaaaaattat
    60481 ttgaagttcg ttcattcgtt acttatgatc tctagaagaa agaaaacgtg aattacttca
    60541 gcgtctagaa ttccagaagg atagtaatca acagaagaaa taatcaatat ccaaatgaaa
    60601 gaagatagct gaatcgagaa aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta
    60661 ctaatcccag agtcgttcgt ttaacattga aatcgatcat atggataaaa cagatttttt
    60721 caccaaccct caatagatga tggagttgct cggatgaatt atttgtgtag atggcataag
    60781 ggagggcgta cagaaatgaa aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat
    60841 cctaaactaa aagatgtgat gcggctatgg cggaattggc agacgcgcta gcttcaggcg
    60901 ctagtgaggg tgacctcgtg gaagttcgag tcttcttagc cgcataattt gtttttttat
    60961 aaagagaagg tacacctgga gcgatgagaa agaaaataac atattatcta aaagaatata
    61021 ggaaaaattt taaacaacgg aaccagattc aaataaatca aaaaaaattt gtgtgaaggt
    61081 gggacagttt aaaacctaat agattgattc aaatagtaga tttatagatt ctgatacaag
    61141 aaatactggc aaacgatagt taaggaataa agactaaact taaactcaaa aagaggtcat
    61201 gcgaaagaat aaatcgcatg acctcttact atattttgaa tgtgtttaaa cgttcaaata
    61261 atgaatctta gtaccaaccg tttgcttccc agaatgcttt cgccgcttcc caagaaccat
    61321 aacgtgaagt aacatattgt tctgctactc tttcttggtt tgcagcagag tagtcaccat
    61381 ttaagtaaga agaatctaat tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag
    61441 aaccgcttga ttctttttgt gcgatccatt cttttgcaga gcttgtactt gctggagtag
    61501 cagcagtttc tgttacagca ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt
    61561 ccgtttgaac cgtttcttgt acaacaggtt gttcagcttg aacaggttca gcttggactg
    61621 gtgcttcttg aacggctgct tgaggttcag cagctgcttg tgcagagcct tctgttggga
    61681 ttgttaattg ttgtcctgag tagatcaggt taatatcaga aattgaattt gattctgcaa
    61741 tggcattgat caaggagtta tcaccaacgt atttttgaga aatcgttgat agagtatcac
    61801 ctgattgaac ggtataagct tcgtccgcat gagcagtcgt tcccatgaag aatgcagcgc
    61861 cggcagccaa agttgttcca aaaagtaaag ttttaagtga tgtcatgaaa aatctccttt
    61921 attcgttaaa aagttttatt tgttgtgttc aacgaggaat actttaacac gtgaaaatat
    61981 ttcataggta aaagaagcat gacaatttat gacaaatcca cgttttttgt aacagaatga
    62041 tgttcgctcg atgtgaaaaa tgatgaggaa tttcacataa tgcagaaata atgggattag
    62101 aacaggtgtt ctgatgagaa aagaaatgta taaataactt gaaagaaatt gcttttgttc
    62161 atcattttat ttacatattg cgtatatttt ttgtattttt gcttgagatg ttacaataaa
    62221 agaaaaagga ggcggttcca atgagtaaaa gataccagaa catcatggta gcagttgacg
    62281 gatcaagaca atcgatcaaa gcgttcgctg aagcccttga cttagcaaag gacaatgaag
    62341 cccacttatt tatcgtgtca atcatcaaca aagttgagct gacccatagt gcctatgcct
    62401 tttcaaaaat ctatgcagat gaaaaacaaa gaacagaagt agccatgctg aaaaaaatca
    62461 atgatgccaa agagtacggc attgctgaga tccacgcaat agttgaaact ggagatccaa
    62521 gaaatttgat tgccaatgtc atccctggac aagaaaatat tgatttgatc gttatgggag
    62581 cgacaggaaa aggtgctata caacaagcat tagttggatc gaccgcttct tatgtcgtat
    62641 cacatgcccc ttgcagtgtg ttggtcgtga agtaatgaga taagggagga atagaaaatg
    62701 gttcgtcctc aagaagtccg tgcgccaaaa gaaaaaatag aaattttagc aattattgaa
    62761 gacggcacac aaacaaagaa gggatactcg atcgctttag taaagtggaa aggaaaaaaa
    62821 ggtgttgctc tgagatggga tggagataac caacaagata aaggattccc tgtcacagct
    62881 aacggttatc atccagcgtg gtttgttctg cctgataaat ttatggaatt atacagctat
    62941 gattatgcaa gagccgcagc atcaatcaaa gcattagata aattaacaga agagcagaat
    63001 gaaatggagg aaaaataaaa aagacgaacc aatcagcctt tagaaaactg attagttcga
    63061 ggagtaaaaa tgaaaaaagg ttatttggga tgaaactaga ataatgcgta taacttaaat
    63121 aaaacttaaa gcaataaaat ggcaagaggg tgaggcaaat ggaagcagga aaagtattac
    63181 aggaagtaaa agcactatgt gcagataaaa aatatgaaga agcgaaaagc ctgatcgaaa
    63241 agaacaaaga aaatttggaa gaaaagtacc cgatcgcaca agaatttata gaattaaaac
    63301 aaggaacgat ccttcaccga ttcaaacatt tttttggtat aacagaatag aaaaaacgat
    63361 taaagatcgg cagaaatttg ctgcatcttt aatcgttttc tttttaaaca tgcgagggat
    63421 tcaattctga ttcttatata aaaaatagat ccaaataata agaaaaatta ctgaaagcgt
    63481 caattcgatg atgtgtgtta ttaatacaga cgttgtttcc caatgcctaa aagaaaaaat
    63541 ttgataagta aaatatacag caggtaaaga ggccagtaaa actgaaaact tcttttttgt
    63601 ccattttatg taatagaaaa gaatgcttat cataataaaa aaaggaatat aaaaaacaaa
    63661 ttgaccgatc gcgatcatac tattgcctcc gataaataaa gtccatgata caaaataaca
    63721 ctttgtgcga aatggcgcaa gcagttttgt ggatttatcc ggtaaaatct gtatcgattt
    63781 tgaaattaga ggagaaactt gctataatca aagtgaaaga aggagtggga ttttcatgga
    63841 tcatgtagtg ttagaaatcg atttgcattt gatcaataat attcgggtga tttactatgt
    63901 tgtaagtaac agtgtcgaac aacgtgtact gacgaataaa ataaatggta ttttagcaaa
    63961 gaaagacgtt caccgattca ataatggtga aggaagttat tatagtatcc cagtagaaaa
    64021 aattatttat acgacagtca aagtcagaga agatctagat accaaaaaag catatgaacc
    64081 tatttttaca acttattgat tgacagcaga tggaggaaaa aaatgaatcc atatgaacaa
    64141 cttattcaag atttgatcga tggaaaaatc gaaaagattg atgtaaaaag agaagaatta
    64201 atggcttttc gagaagcgtg gctgaagttg gagaatcgta aatatgtggt gggaaaagcc
    64261 tcactgaacg gcaatgtgac ttatcattac gatccgacac gtttatttta gtatgaagta
    64321 ttcagaaaag aaccaaagga ggacgatgca gcagcattgt cctcctttga ttttacaaaa
    64381 gtgaaagttt tacttatttg aatagatttt taaatttgtt tttgacactg tcgatattta
    64441 catcttttaa aagattttgg gctttctctt tataaggtcc taattgttct ttatgttctt
    64501 cgataaatcg ttttgcgcca tcgaaattgc cgttcttaat catttccttg actttttgaa
    64561 ataattcttc ttgattcatt ttgaacacat cctttgctat caacatgatt ttttacatgg
    64621 acaaaacgag aaaaactgat ttatccacac gtcaataata tcaaataacg atcattttta
    64681 ctaaaaaaac actcgattct cgcttctaaa gtaggagaaa aatctccaac ttgggacgga
    64741 tggaatttct ttaactgacc catatactga atatataaca aggaggtaga aagatgaaag
    64801 tgattaaata tttgattgga ctggcggcga ttagtggtat tttgctattt ggactgaaat
    64861 attatactga aggaagttat ggagaaatcc ctggtatact tgatcagcta aatccattag
    64921 ttgaaaaagg agaggtctat gtaaagacac aaaaaccaga ggaagtaaat gaatatggta
    64981 cagcaaggta tgtgcagaaa gctgctgatg ccaatgggaa agaacgaacg gttgaattta
    65041 atggtctttc tgtgttaaaa gaaaatcatt atttgaaaat tagcaataaa ggagcacaca
    65101 tagaaacgta tgaggaagtt cctagagaaa aagttccgga gaaagcatta gcaattattg
    65161 ggtaaatcag tttttgtatt aagaatacgc ccgattttat tattcataat ttaattatgt
    65221 aaactaaatt ctagtagtaa aaataataca ataatccccc tgcaaaaaga ttgttctttt
    65281 acagggggtt cattttttca gcaagcacat caaattggga tggcagggtt cgaacctaca
    65341 attgcttgat tcagagtcaa gtgccttacc agtttggcta catcccattt attcaacaaa
    65401 ataatcatag cactttccta aaaaaacgca agttattttt cttgatcgga tagttgttgc
    65461 tttactcgta aaaggcggcg tactagtttc ttttctaatc cagcaggaag ggcaatcagt
    65521 tctgctactg acaagacggt gtccaataac tgatattctt tgacaggttc attcataata
    65581 tgatagatgt ttgctaaacg ttcaatatat tcaaagcgag tatactctgt tcgagcaatt
    65641 agaaatttca ataaatcggt ggctttctcg tactcttgat ctgcgtaata aacaagagat
    65701 tgacgataca atttgatttc cagttcattg agggtagcag gtctgcgacg ctcgttccac
    65761 tcattaatag agactttacc ttgaacattt cccttgcagt cacggacaaa aataatcgga
    65821 atttcatttg gatccatttt tttatgtgtg aatttttcca ttatctacgc tcccttcaaa
    65881 ggtttggtat cttgagtata agggattttt gtttgaatta ctagcttttc tccaaagtta
    65941 gcatctgtcc acccattgca gtgaaaatcg ccgaaaaatc gttgatcggt actgctcgat
    66001 tttttacacc atagggatcg ttgacataaa tattttcttt gtcatatcca gtgactacac
    66061 aagcatgaac aagaggagtg actcttactt ctccattatt tgtggtccaa gtcataaaat
    66121 cagtttcatc tgggacttca aaattgatag tcacagaagt ccagacagga tttcctttag
    66181 atagtgcttt tttgatatca tcgagttgag catcggtttt tggaaaagtg tgataatccg
    66241 tttggacgat ttgctgagca agggaggcaa ctgggggaac atagacgacc atagcccagt
    66301 ctccgccact gatatcacca acaaatcctt cattaggatc gccatgagtc ccatctgcat
    66361 tgaaaacagg gacgtaatcc aattgctcag ctaactggtt tttagttgtt tcataaccat
    66421 aaaaagataa aagcatagaa agcgaagtga cttcacagcc attttctaaa gaaggagaat
    66481 caaactggct ctctaatgga acatctagta aaacttctgc tttctctttt gtctttttcg
    66541 ttttttgtgt tgaacttttt tgtgcagtgt tcattgtatg ttctggagaa tggatatgta
    66601 ggtaataact tgtaccgctt aataaaagag cagctgtgcc ccctagaaac aatagttttt
    66661 tgaacgtttt tttcttcatt tgaaatctcc cttttcagct gtttatcttt cctattataa
    66721 atgtattttt attggaaatg gaagtattat attttaatat gtaggaaata attagttttt
    66781 aaggaatatg ttttaatttt ttgaaaaaaa ttcttctaaa tacaacagct gttttaattg
    66841 tgaaaacgaa aacagtacgc tataatgcct gtaaggttga tttttaactg taaataaaaa
    66901 tcaatgagtt aaatctccaa agaaatagca gtggtaatgt ggagaaaagt tccagcagtt
    66961 taggaggatt ttgatgacgg aagaaatgat taatttagga gaacaatatg catgcaaacc
    67021 aattggtttt acaaaaacgg tcattggtga agttgtatca aaaatgacaa actgcgccgt
    67081 ggtcaaagtt gcccaatgtg ctgcagagga ccaagaatta ttagatgaaa aagcaagcat
    67141 ggttgttgca aaatatgata catttgaata aagaaaacaa ttggacgtgc ggcgtgctgc
    67201 aagtccaatt tctttgagaa ggtaaatcag tattcttttc ctattttctg gagaagaaaa
    67261 agcgaggcga gttgtcatgg taacattctt tacggttcta ttgtttttat tcgttgttgt
    67321 gatgttttta tttctttggc tggttcgaaa agaaattatt tatcggactg tgcgaaatcg
    67381 atgggtctac ttggtgatac ccttcttagt gtcactagtc atttggtata cactcatcag
    67441 tcatcctaca gcagatgaat tggcaaaagg gatcttgtca gcaatgattt ttattagttt
    67501 tttgttggac agtagaggaa tcacagaaga aggacttgtt ttgaatagct ttgacaaaaa
    67561 aggtgttcct ttttctgaga tcaataaaat tgtattatat cagccaaaag gttctaaaat
    67621 cgtaaaaatg aattttttcc gaaacggatg gcgaggaccg atgctgaaat tttctgcatc
    67681 attagaagaa ttagttccat ttttgtccca acgcttaaac gatgaggcag agattgatat
    67741 tatgattgat ccagaataaa aacagaggtt gcgacaagat gtttgtcaca acctctgttt
    67801 tttatttcaa ataaaattat ccaaatacgc ggaagcgatc ggcatcgttc atgtattctt
    67861 tttccccagc cggtgtttct ggtgaaccga atactaattg cgaacgtagt ttccagtttg
    67921 aagggatatt ccattctttt gcaactgctt cgtcaataac tgggttgtaa tgttgcaagt
    67981 ttgcgcccaa tcctttatca actaatgcta cccaagtatt agctgttgcg attccgtttg
    68041 attgttctga ccaatctggg aagttgtctg catataattc aaattgttct tgtagacctt
    68101 ttaccacgtc agtatctttg aagaataata ctgtaccata accatttttg aaaccagcta
    68161 atttgttttg tgtatttggg aaagcttctg ctggagtcaa tgggcgaagt gcttcttcag
    68221 tgatttccca aagtttttca tgtgcatctc cgaaaaggat aactgcgcgt gttgattgag
    68281 cattgaaagc agttgggctt tctttaattg cttctttgat caaagttgtt aattcttcat
    68341 tagaaagtga aacattacgt cctagatgat agattgaacg gcggtttttc aaagtgtcag
    68401 taaaagttgt cataataaaa tttctccttt gatttgcgtt ttgcattttt ttaattgatt
    68461 acgaaatgaa gtatagcacc ttacaaaaag taagtaaaca gatatgctca caaaaaataa
    68521 gcgaagttca aaaaacaatt ttaataagaa aaatcgttat gtaaggattt tcttagttat
    68581 cgctttcatt gttgtgtttt aggaattttt gcttatttga aaaaaacagc tgatatataa
    68641 ttattaaatc tctatcttct tttttttatg atattgccat taaaaattac ttgcttttat
    68701 agaaaattag tttatatttc ttaaataagt tttttagtta aatttttcgg taaagatgta
    68761 taatattttc tgaaaattta aagaattggg gtatagatta tggaaaaaca gcaagacaag
    68821 ggcttcttcg ggcagccgaa gggattgtca actttgttct tcactgagat gtgggaacga
    68881 tttagttatt acggtatgcg tgcattactt atctattata tgtacgatac agttgccaac
    68941 gggggacttg gattaccacg agcaacagca ttagctatca tgtcgatcta cggatctttg
    69001 atctacatgt ctagtatcgt cggcggatgg gtatctgacc gagtgttagg agcaaaacgc
    69061 actgtattct tcggtggagt tttcattatg cttggccaca tcgtcttggc tttaccattt
    69121 ggtgtaagta cattgtttgt ttcgatggca ttgattatta tcggaaccgc ctttttgaaa
    69181 ccaaatgttt ctggcatggt cggacatctt tattcaaaga ctgacttgcg aagagatgca
    69241 ggattttcga ttttttacat gggtgtcaac ttaggggcat tattagcacc actaatcgtt
    69301 ggtacaatcg gtcaagaata taactatcat ttagggttct ctttagctgc tattgggatg
    69361 ttctttggat tattacaata catcatccaa ggacgcaaga cattagacgg agtgggtaca
    69421 acaccgccga atcctttgac aactgaagaa cgtaaaggat tcatccgtaa cttgatcatc
    69481 gcacttgttg ttatcgctgc agtattcggc ggggcacaac taactggtca tctgacaatt
    69541 gacttttttg tcaatacaat cagtgtattg gggattatct taccgttgta ttactttatc
    69601 aaaatgctga cttcaaaaga tgtaactgct gaagaaaaac caagagtatg ggcttatatc
    69661 ccattattcc ttgcagcaat cattttctgg tcattacaag aacaaggttc atctatctta
    69721 gctttatttg ctagtgaacg tacacaatct acgctatttg gtgtaacgat ccaagcaagc
    69781 tggtatcaat cattgaatcc aattttcatc tttattctga caccagtatt cgtaacgatg
    69841 tggacgaaac ttggtgaacg tcaaccatct acagtagtga aattctctct tggactttta
    69901 tttgcaggat tatcgtactt gctattgatg ttgccgggta tgctttatgg tacaagtgga
    69961 aaagtaagtc cattgtggtt gttgggaagt ttcttcatcg tggttattgc tgaattgtgt
    70021 ctttcaccag ttggtttgtc tgtgacaaca aaattagcac cacgagcatt tgaatcacaa
    70081 acaatcgcta tttggttctt agctgatgct tcttcacaag caatcaatgc acaaattgca
    70141 cgcttctaca caccgggaac agaatctgct tacttcggta ttatcggtgc agtagcaatc
    70201 gtcggtggta tcttgctatt tactgtgaaa gaaccaatca aaaaactaat gggctctatt
    70261 cattaatcaa tgaacataaa aacaggaatt gcttatttgc agcagttcct gtttt
//
LOCUS       DACDRN010000008        61086 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDRN010000008 DACDRN010000000
VERSION     DACDRN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.008"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            212..604
                     /locus_tag="IWJ80_000718"
     CDS             212..604
                     /locus_tag="IWJ80_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9460478.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="IWJ80_000719"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="IWJ80_000719"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP9460479.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="IWJ80_000720"
     CDS             3165..4775
                     /locus_tag="IWJ80_000720"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP9460480.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="IWJ80_000721"
     CDS             5067..5936
                     /locus_tag="IWJ80_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP9460481.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="IWJ80_000722"
     CDS             6101..7372
                     /locus_tag="IWJ80_000722"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9460482.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /locus_tag="IWJ80_000723"
     CDS             7369..8664
                     /locus_tag="IWJ80_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP9460483.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="IWJ80_000724"
     CDS             8763..9026
                     /locus_tag="IWJ80_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP9460484.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="IWJ80_000725"
     CDS             9471..9920
                     /locus_tag="IWJ80_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9460485.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="IWJ80_000726"
     CDS             9977..11395
                     /locus_tag="IWJ80_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9460486.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="IWJ80_000727"
     CDS             11395..12255
                     /locus_tag="IWJ80_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP9460487.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="IWJ80_000728"
     CDS             12429..13202
                     /locus_tag="IWJ80_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9460488.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="IWJ80_000729"
     CDS             13349..13714
                     /locus_tag="IWJ80_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP9460489.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="IWJ80_000730"
     CDS             complement(13862..15070)
                     /locus_tag="IWJ80_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9460490.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="IWJ80_000731"
     CDS             complement(15089..15367)
                     /locus_tag="IWJ80_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460491.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="IWJ80_000732"
     CDS             15560..16216
                     /locus_tag="IWJ80_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9460492.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="IWJ80_000733"
     CDS             complement(16658..17455)
                     /locus_tag="IWJ80_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP9460493.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="IWJ80_000734"
     CDS             complement(17452..18636)
                     /locus_tag="IWJ80_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9460494.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="IWJ80_000735"
     CDS             complement(18661..19908)
                     /locus_tag="IWJ80_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP9460495.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="IWJ80_000736"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="IWJ80_000736"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP9460496.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="IWJ80_000737"
     CDS             21888..22202
                     /locus_tag="IWJ80_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP9460497.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="IWJ80_000738"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="IWJ80_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP9460498.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="IWJ80_000739"
     CDS             22887..23531
                     /locus_tag="IWJ80_000739"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP9460499.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="IWJ80_000740"
     CDS             23546..23875
                     /locus_tag="IWJ80_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460500.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="IWJ80_000741"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="IWJ80_000741"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP9460501.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="IWJ80_000742"
     CDS             24863..25687
                     /locus_tag="IWJ80_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP9460502.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="IWJ80_000743"
     CDS             25680..26027
                     /locus_tag="IWJ80_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP9460503.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="IWJ80_000744"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="IWJ80_000744"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP9460504.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="IWJ80_000745"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="IWJ80_000745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP9460505.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IWJ80_000746"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IWJ80_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP9460506.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IWJ80_000747"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IWJ80_000747"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP9460507.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="IWJ80_000748"
     CDS             31684..32451
                     /locus_tag="IWJ80_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9460508.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="IWJ80_000749"
     CDS             32441..34483
                     /locus_tag="IWJ80_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9460509.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="IWJ80_000750"
     CDS             34764..34913
                     /locus_tag="IWJ80_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP9460510.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="IWJ80_000751"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="IWJ80_000751"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP9460511.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="IWJ80_000752"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="IWJ80_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP9460512.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="IWJ80_000753"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="IWJ80_000753"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP9460513.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="IWJ80_000754"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="IWJ80_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP9460514.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="IWJ80_000755"
     CDS             39330..40412
                     /locus_tag="IWJ80_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP9460515.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="IWJ80_000756"
     CDS             40412..41215
                     /locus_tag="IWJ80_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9460516.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="IWJ80_000757"
     CDS             41285..42025
                     /locus_tag="IWJ80_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9460517.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="IWJ80_000758"
     CDS             42036..43040
                     /locus_tag="IWJ80_000758"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP9460518.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="IWJ80_000759"
     CDS             complement(43085..44929)
                     /locus_tag="IWJ80_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460519.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45141..45659
                     /locus_tag="IWJ80_000760"
     CDS             45141..45659
                     /locus_tag="IWJ80_000760"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9460520.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45787..46470
                     /locus_tag="IWJ80_000761"
     CDS             45787..46470
                     /locus_tag="IWJ80_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP9460521.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46779..46852
                     /locus_tag="IWJ80_000762"
     tRNA            46779..46852
                     /locus_tag="IWJ80_000762"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46813..46815,aa:Arg,seq:tct)
     gene            47011..47370
                     /locus_tag="IWJ80_000763"
     CDS             47011..47370
                     /locus_tag="IWJ80_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP9460522.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47493..49124
                     /locus_tag="IWJ80_000764"
     CDS             47493..49124
                     /locus_tag="IWJ80_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP9460523.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49186..49827)
                     /locus_tag="IWJ80_000765"
     CDS             complement(49186..49827)
                     /locus_tag="IWJ80_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9460524.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            50109..50417
                     /gene="rplU"
                     /locus_tag="IWJ80_000766"
     CDS             50109..50417
                     /gene="rplU"
                     /locus_tag="IWJ80_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP9460525.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50444..50788
                     /locus_tag="IWJ80_000767"
     CDS             50444..50788
                     /locus_tag="IWJ80_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP9460526.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50801..51094
                     /gene="rpmA"
                     /locus_tag="IWJ80_000768"
     CDS             50801..51094
                     /gene="rpmA"
                     /locus_tag="IWJ80_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP9460527.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            51501..54335
                     /locus_tag="IWJ80_000769"
     CDS             51501..54335
                     /locus_tag="IWJ80_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9460528.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            54526..55017
                     /locus_tag="IWJ80_000770"
     CDS             54526..55017
                     /locus_tag="IWJ80_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP9460529.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            55120..56109
                     /gene="manX"
                     /locus_tag="IWJ80_000771"
     CDS             55120..56109
                     /gene="manX"
                     /locus_tag="IWJ80_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP9460530.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            56142..56945
                     /locus_tag="IWJ80_000772"
     CDS             56142..56945
                     /locus_tag="IWJ80_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP9460531.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            56966..57880
                     /locus_tag="IWJ80_000773"
     CDS             56966..57880
                     /locus_tag="IWJ80_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP9460532.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVERWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            57963..58337
                     /locus_tag="IWJ80_000774"
     CDS             57963..58337
                     /locus_tag="IWJ80_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP9460533.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            58476..58721
                     /locus_tag="IWJ80_000775"
     CDS             58476..58721
                     /locus_tag="IWJ80_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9460534.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            58893..59957
                     /locus_tag="IWJ80_000776"
     CDS             58893..59957
                     /locus_tag="IWJ80_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9460535.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            60203..60628
                     /locus_tag="IWJ80_000777"
     CDS             60203..60628
                     /locus_tag="IWJ80_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9460536.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            60621..61070
                     /gene="nusB"
                     /locus_tag="IWJ80_000778"
     CDS             60621..61070
                     /gene="nusB"
                     /locus_tag="IWJ80_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP9460537.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
BASE COUNT        19641 a        10490 c        12861 g        18094 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat
    45121 aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa
    45181 cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt
    45241 tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga
    45301 tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg
    45361 aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag
    45421 atttttatac aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa
    45481 atattatcgg tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg
    45541 catttgcatg gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag
    45601 catggaatac ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag
    45661 aatgttaaaa acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga
    45721 gaaaacactc atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg
    45781 agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt
    45841 atgcgtaccc agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat
    45901 gtagcagaag taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga
    45961 cagcttcagc ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc
    46021 atggccacga caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg
    46081 atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt
    46141 gaaggtcaag cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg
    46201 gatagtgcaa aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa
    46261 ggtggcgttt tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa
    46321 gagattcccc gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg
    46381 atggatcatc ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg
    46441 acaaaggaat cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg
    46501 attgaatcga gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta
    46561 aaggatacag gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc
    46621 aattgataac cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag
    46681 ttgggccaaa ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa
    46741 tttaattatc tataattatt gatgttccgc tgattcttgt catagtagct cagctggata
    46801 gagcatccgc cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa
    46861 ccgttagaaa ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt
    46921 ttcaaaggca aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa
    46981 ataaaactca tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact
    47041 ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac
    47101 acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca
    47161 ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct
    47221 tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt
    47281 gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct
    47341 ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag
    47401 gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt
    47461 ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa
    47521 aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca
    47581 cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg
    47641 cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg
    47701 gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg
    47761 caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg
    47821 cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg
    47881 ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg
    47941 ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc
    48001 cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc
    48061 caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag
    48121 ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca
    48181 gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc
    48241 cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg
    48301 gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa
    48361 atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg
    48421 tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac
    48481 tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag
    48541 gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta
    48601 ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct
    48661 ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca
    48721 aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag
    48781 aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt
    48841 cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct
    48901 ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag
    48961 aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa
    49021 aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg
    49081 cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac
    49141 agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa
    49201 aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa
    49261 aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc
    49321 atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt
    49381 aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa
    49441 ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga
    49501 acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg
    49561 atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt
    49621 taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac
    49681 atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag
    49741 aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc
    49801 ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat
    49861 aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt
    49921 attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt
    49981 ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta
    50041 ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc
    50101 tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac
    50161 aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag
    50221 ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg
    50281 ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac
    50341 ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg
    50401 acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt
    50461 taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg
    50521 tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa
    50581 cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg
    50641 ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat
    50701 tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat
    50761 ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct
    50821 acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc
    50881 aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta
    50941 ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac
    51001 tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca
    51061 agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt
    51121 gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag tggaggctac
    51181 gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat caggatgttt
    51241 tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg aaataattat
    51301 atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg atttttcgtt
    51361 cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa tcaggtaaat
    51421 agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt acaatagtct
    51481 atagaaaagg agcgatacca ttggcaaaac gcattgaccg aatttatatg tatgtcaaag
    51541 aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca caggaaattg
    51601 ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag ctagtgcgcg
    51661 aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa acactcgtta
    51721 cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg gtctctgaag
    51781 aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt ggagctaacg
    51841 gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg ccaagaggat
    51901 taaattgttt gattacagga cctaccggat caggaaagac ttattttgca cacgcgatgt
    51961 atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt gttttcaatt
    52021 gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg tacgcaaaag
    52081 gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg gatggcggta
    52141 tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg atcttttact
    52201 ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat gaagcaaatg
    52261 tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa acttttgttc
    52321 ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca aatgagaaag
    52381 tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga aagatttctt
    52441 tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat ataggacaat
    52501 tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg caatcacccg
    52561 aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg attcaattag
    52621 caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag ataactgtag
    52681 caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct tataatctat
    52741 atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat caagaagcaa
    52801 tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat aaagaccatg
    52861 gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa atcatagaag
    52921 tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt cagcaaaact
    52981 ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac ttaggagaag
    53041 aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat gaatatgctg
    53101 ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt ccagaaagtg
    53161 aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca agcggcagaa
    53221 tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc caagttgtcc
    53281 aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat atggatccga
    53341 aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga agcggggtca
    53401 tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa agagacactg
    53461 gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa actgtacgaa
    53521 aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa aattttcgag
    53581 gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt cataagaaaa
    53641 aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc aaagaattga
    53701 tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct ttgtcgattg
    53761 tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata gctactactg
    53821 ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt attgatcaaa
    53881 atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa actgaattca
    53941 tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct gataacttca
    54001 tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca caaatctctc
    54061 agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg atgcacacag
    54121 caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa aacgaattga
    54181 caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt ctgttggaag
    54241 atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt ctagtccata
    54301 atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaacgat acacttttat
    54361 agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg tatcgtaaaa
    54421 aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt cacacagaaa
    54481 tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga tattcgattg
    54541 gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc gaagcaaaca
    54601 ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt gagaaagttt
    54661 ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt gcagaaaatg
    54721 attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt attcaccaat
    54781 ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt gaatatcggc
    54841 ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt aaatcaacaa
    54901 gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat tcgaaaagta
    54961 cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa ggtgtagcaa
    55021 gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt attaatattt
    55081 ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat tatcctagca
    55141 agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt tggcgaacaa
    55201 gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt gagagccaaa
    55261 ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt agttgattta
    55321 tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa agataaatgg
    55381 gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc acgcttttca
    55441 atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga cggtgtaaaa
    55501 gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga agacgcacaa
    55561 cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa atatgtatta
    55621 gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac aaaagcagtg
    55681 caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct gcgtaagaga
    55741 ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat tagcaaaatg
    55801 atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt atttgaaaat
    55861 cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt aaatgttggg
    55921 tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat gggacaagaa
    55981 gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt acgtaaagta
    56041 cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc tgaattagca
    56101 aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt atttcaataa
    56161 ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat gaatttcaat
    56221 ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac ctcgaagctg
    56281 gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat atcggtgccg
    56341 ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta gttttaggtg
    56401 gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg gctgttgcag
    56461 ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat ttgatggatg
    56521 cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt gctgtatgtt
    56581 tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca gcacaaactg
    56641 tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct atcggtgggg
    56701 gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca aaagaagtat
    56761 ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca ttgattgctc
    56821 taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa atgggtggtt
    56881 catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt ttgaatgact
    56941 attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat caaaaaaaga
    57001 tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact acgaacgtat
    57061 gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat acaaaacaaa
    57121 agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac acccatacat
    57181 tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga atggcgcgcc
    57241 tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt tagctggtgt
    57301 tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag gtgcttcatt
    57361 agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga acattattcg
    57421 ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta aaattactga
    57481 tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt tagggatgtt
    57541 cgtattagct gcccttgtag aaagatgggt atcaatcaaa ttcttaccag ttgtatcaac
    57601 tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag gtggtgaagg
    57661 aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg aaaaggtaac
    57721 aacattacaa gacaacttag accaattgat tcctggttta gctgcattag gattaacatt
    57781 gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc ttggtttatt
    57841 cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa tatagctcgg
    57901 gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc tggagggaga
    57961 aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact tcgtttacgg
    58021 gtctgacaga ttacgggcaa atcatgatag gtgataatgg ttttgagttt taccatgcgc
    58081 gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta ctagcatcag
    58141 tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa aatggtacat
    58201 acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa tatgtagatg
    58261 aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt attcgtagta
    58321 tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct ttattttttt
    58381 actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt aaatcccata
    58441 tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg acattgattg
    58501 ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat tcgatgggct
    58561 gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg attttcggcg
    58621 aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc gcagtgattt
    58681 tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca aaatagtaaa
    58741 ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt aaaataaaaa
    58801 gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg ctataatgga
    58861 aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa aaattgcgca
    58921 aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac aatcttcgtt
    58981 acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa aaagcatttt
    59041 ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg tttgaaatta
    59101 ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa gaagaactgc
    59161 actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt ttagaagaaa
    59221 tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga gaagtgaaag
    59281 atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg gcatatgatc
    59341 acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat caactagact
    59401 tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt gctagcggat
    59461 tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa ggtgacttga
    59521 tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca cgtacattcg
    59581 ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta gatgctcaat
    59641 tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat gcagttgcta
    59701 gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca ggacatggga
    59761 tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag caatttgtac
    59821 caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt ggagtacgta
    59881 ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt tcaccaaaag
    59941 aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca gtgttcacag
    60001 actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt ttaagctgta
    60061 agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg atcggcggca
    60121 gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta tctcgtgctt
    60181 aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa acgcaaacca
    60241 agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg ggattgcagc
    60301 ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg tcactgaatt
    60361 attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag atggcttgaa
    60421 agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag caatggaaat
    60481 gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat ctgaagtcaa
    60541 tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg aagaactttt
    60601 tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt cgggaaatgg
    60661 cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa gatgcgattt
    60721 tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca gagtttgttc
    60781 ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa ttggataaag
    60841 tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa atggatttgg
    60901 tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca gcggctgttg
    60961 ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca cgtaaattcg
    61021 tcaacggtgt tttatccaat gtattgaaag aattagaagc tggggcgtaa gtcctagctt
    61081 ttttct
//
LOCUS       DACDRN010000009        53849 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDRN010000009 DACDRN010000000
VERSION     DACDRN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.009"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            11..733
                     /locus_tag="IWJ80_000779"
     CDS             11..733
                     /locus_tag="IWJ80_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9460538.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            791..2830
                     /locus_tag="IWJ80_000780"
     CDS             791..2830
                     /locus_tag="IWJ80_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP9460539.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2939..4090)
                     /locus_tag="IWJ80_000781"
     CDS             complement(2939..4090)
                     /locus_tag="IWJ80_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9460540.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4244..4774)
                     /locus_tag="IWJ80_000782"
     CDS             complement(4244..4774)
                     /locus_tag="IWJ80_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460541.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4873..5223)
                     /locus_tag="IWJ80_000783"
     CDS             complement(4873..5223)
                     /locus_tag="IWJ80_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP9460542.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5380..6909)
                     /gene="ahpF"
                     /locus_tag="IWJ80_000784"
     CDS             complement(5380..6909)
                     /gene="ahpF"
                     /locus_tag="IWJ80_000784"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP9460543.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6919..7482)
                     /gene="ahpC"
                     /locus_tag="IWJ80_000785"
     CDS             complement(6919..7482)
                     /gene="ahpC"
                     /locus_tag="IWJ80_000785"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP9460544.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7626..8288)
                     /locus_tag="IWJ80_000786"
     CDS             complement(7626..8288)
                     /locus_tag="IWJ80_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9460545.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8472..10118
                     /locus_tag="IWJ80_000787"
     CDS             8472..10118
                     /locus_tag="IWJ80_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9460546.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10109..10237
                     /locus_tag="IWJ80_000788"
     CDS             10109..10237
                     /locus_tag="IWJ80_000788"
                     /inference="COORDINATES: protein motif:HMM:NF015727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460547.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDHRKKRLP"
     gene            10204..11268
                     /gene="uxuA"
                     /locus_tag="IWJ80_000789"
     CDS             10204..11268
                     /gene="uxuA"
                     /locus_tag="IWJ80_000789"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9460548.1"
                     /translation="MDGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPRERIKEL
                     KEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVICYNFMP
                     VFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEPERLAHI
                     KSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLPRIVKNR
                     EDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRNIKFLAN
                     RDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGLYDRALG
                     AVYLNGLWEAIEKGNKSKDN"
     gene            complement(11376..12929)
                     /locus_tag="IWJ80_000790"
     CDS             complement(11376..12929)
                     /locus_tag="IWJ80_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9460549.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            13077..14365
                     /locus_tag="IWJ80_000791"
                     /pseudo
     CDS             13077..14365
                     /locus_tag="IWJ80_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(14491..15570)
                     /locus_tag="IWJ80_000792"
     CDS             complement(14491..15570)
                     /locus_tag="IWJ80_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9460550.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15673..16113)
                     /locus_tag="IWJ80_000793"
     CDS             complement(15673..16113)
                     /locus_tag="IWJ80_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9460551.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16170..16580)
                     /locus_tag="IWJ80_000794"
     CDS             complement(16170..16580)
                     /locus_tag="IWJ80_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9460552.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16760..17194)
                     /locus_tag="IWJ80_000795"
     CDS             complement(16760..17194)
                     /locus_tag="IWJ80_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9460553.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(17294..18142)
                     /locus_tag="IWJ80_000796"
     CDS             complement(17294..18142)
                     /locus_tag="IWJ80_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9460554.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            18531..20297
                     /locus_tag="IWJ80_000797"
     CDS             18531..20297
                     /locus_tag="IWJ80_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP9460555.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            20399..21658
                     /locus_tag="IWJ80_000798"
     CDS             20399..21658
                     /locus_tag="IWJ80_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9460556.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            21805..23106
                     /locus_tag="IWJ80_000799"
     CDS             21805..23106
                     /locus_tag="IWJ80_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9460557.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            23109..23960
                     /locus_tag="IWJ80_000800"
     CDS             23109..23960
                     /locus_tag="IWJ80_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9460558.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            23973..24788
                     /locus_tag="IWJ80_000801"
     CDS             23973..24788
                     /locus_tag="IWJ80_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP9460559.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            24939..26105
                     /locus_tag="IWJ80_000802"
     CDS             24939..26105
                     /locus_tag="IWJ80_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9460560.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            26335..26982
                     /locus_tag="IWJ80_000803"
     CDS             26335..26982
                     /locus_tag="IWJ80_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP9460561.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            27066..28010
                     /locus_tag="IWJ80_000804"
     CDS             27066..28010
                     /locus_tag="IWJ80_000804"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP9460562.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            28145..29596
                     /locus_tag="IWJ80_000805"
     CDS             28145..29596
                     /locus_tag="IWJ80_000805"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP9460563.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            29617..30453
                     /locus_tag="IWJ80_000806"
     CDS             29617..30453
                     /locus_tag="IWJ80_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9460564.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            30453..31112
                     /locus_tag="IWJ80_000807"
     CDS             30453..31112
                     /locus_tag="IWJ80_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9460565.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            31238..31927
                     /locus_tag="IWJ80_000808"
     CDS             31238..31927
                     /locus_tag="IWJ80_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460566.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            31933..32889
                     /locus_tag="IWJ80_000809"
     CDS             31933..32889
                     /locus_tag="IWJ80_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP9460567.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            32933..33445
                     /locus_tag="IWJ80_000810"
     CDS             32933..33445
                     /locus_tag="IWJ80_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9460568.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(33701..34249)
                     /locus_tag="IWJ80_000811"
     CDS             complement(33701..34249)
                     /locus_tag="IWJ80_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9460569.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(34338..35519)
                     /locus_tag="IWJ80_000812"
     CDS             complement(34338..35519)
                     /locus_tag="IWJ80_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP9460570.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(35755..37263)
                     /locus_tag="IWJ80_000813"
     CDS             complement(35755..37263)
                     /locus_tag="IWJ80_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP9460571.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            37422..37613
                     /locus_tag="IWJ80_000814"
     CDS             37422..37613
                     /locus_tag="IWJ80_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460572.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            37816..38733
                     /locus_tag="IWJ80_000815"
     CDS             37816..38733
                     /locus_tag="IWJ80_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP9460573.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            38726..39799
                     /locus_tag="IWJ80_000816"
     CDS             38726..39799
                     /locus_tag="IWJ80_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460574.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            39947..40465
                     /locus_tag="IWJ80_000817"
     CDS             39947..40465
                     /locus_tag="IWJ80_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460575.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            40446..40838
                     /locus_tag="IWJ80_000818"
     CDS             40446..40838
                     /locus_tag="IWJ80_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP9460576.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            40914..41849
                     /locus_tag="IWJ80_000819"
     CDS             40914..41849
                     /locus_tag="IWJ80_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP9460577.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            41982..42761
                     /locus_tag="IWJ80_000820"
     CDS             41982..42761
                     /locus_tag="IWJ80_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP9460578.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            42754..43071
                     /locus_tag="IWJ80_000821"
     CDS             42754..43071
                     /locus_tag="IWJ80_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9460579.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            43315..44925
                     /locus_tag="IWJ80_000822"
     CDS             43315..44925
                     /locus_tag="IWJ80_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9460580.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            45082..45930
                     /locus_tag="IWJ80_000823"
     CDS             45082..45930
                     /locus_tag="IWJ80_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9460581.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            46161..46640
                     /locus_tag="IWJ80_000824"
     CDS             46161..46640
                     /locus_tag="IWJ80_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460582.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            46673..48901
                     /locus_tag="IWJ80_000825"
     CDS             46673..48901
                     /locus_tag="IWJ80_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9460583.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            49030..50673
                     /locus_tag="IWJ80_000826"
     CDS             49030..50673
                     /locus_tag="IWJ80_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9460584.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(50746..51291)
                     /locus_tag="IWJ80_000827"
     CDS             complement(50746..51291)
                     /locus_tag="IWJ80_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP9460585.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(51314..51799)
                     /locus_tag="IWJ80_000828"
     CDS             complement(51314..51799)
                     /locus_tag="IWJ80_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9460586.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(51789..52601)
                     /locus_tag="IWJ80_000829"
     CDS             complement(51789..52601)
                     /locus_tag="IWJ80_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP9460587.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(52740..>53849)
                     /locus_tag="IWJ80_000830"
     CDS             complement(52740..>53849)
                     /locus_tag="IWJ80_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9460588.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        17933 a         9795 c        10401 g        15720 t
ORIGIN      
        1 attatctatt ttgttactgc ttacattagt agttggctat ttttcgattg agtttatcca
       61 tggattttcg tctgcaaaac agagttcaac tgtgaaaaag gtagagccga aaagtatccc
      121 taccacacta aatgtggctt tgattggttc agatgctcgt tcgaaagacg aaaacggtcg
      181 ctcggattca cttctggttg cacaatacaa ccagaaaaca caacaagcaa aactgatctc
      241 tatcatgaga gattcatatg tcgatattcc aggttacgga atgaataaga taaatgcagc
      301 ttactcttat gggggagttg atttattaaa tcaaacatta aaggaaaatt tcaaatttga
      361 agctccctat tatgcaagta tcacatttca agattttatc gattgcgtta atgaactgtt
      421 tcctggtgga gtcaagatcg atgcagaaaa atctctagat ttagatggcg tatatataaa
      481 caaaggagag caagtgatgg atggtaatac tttactccag tatgctcgat ttcgggaaga
      541 caaagaagga gacttcggga ggatcagaag gcagcagcaa gtgatcaaag ctatctctca
      601 gcagctgaaa gacgtaactt caatattcaa gctacccaaa gcggttggga aattacttgg
      661 tagtatccaa acgaaccttc cagaaagtgt gcttctggat tgtggtatgg atttcctcaa
      721 agacatatgt tagaataaac aggtataaat agtgaaataa aggaatgaca agcaagagaa
      781 ggaggaaaaa atgaaaagaa gtgacaagca cggcaaaaat cgaacaggcg cttatattgc
      841 cggcgcagtg atcttaatag cagctgcggg tggcggttat ttttactacc agcactatca
      901 agaaacccaa gcagtagaag ctggagaaaa gacagttgag caatttgtcc aagctttgaa
      961 caagggagat tataacaaag ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc
     1021 attatctgaa aaagagatct tagataaata ccagaatata tacggtgctg ctgatgtcaa
     1081 aggacttcag atatcgaatc taaaagtaga taaaaaagac gattctactt acagtttttc
     1141 atataaagca aagatgaata cctcattagg tgaattgaaa gatctttctt ataaaggaac
     1201 attagacaga aatgatggtc aaaccacgat caactggcaa cccaacttgg tttttccaga
     1261 aatggaagga aatgacaaag taagcctgac cacacaagaa gcagcaagag ggaatattat
     1321 agatcgaaat ggtgaaccat tagcaacaac aggcaaacta aaacaattag gagtcgttcc
     1381 aagtaaactt ggagatggag gcgaaaaaac agccaatatc aaagcgattg cttcctcctt
     1441 cgacttaaca gaagatgcta tcaatcaggc gatttcacaa agctgggtac aacccgatta
     1501 ctttgtccca ttgaaaatca ttgatggagc aacgccagaa cttccagctg gagctaccat
     1561 tcaagaagta gacggcagat attatccttt gggtgaagca gctgctcagc tgattggtta
     1621 cgtgggagat atcacagcag aagatattga taagaatcca gaattaagca gtaatggaaa
     1681 aatcggtcgc tcaggtttgg agatggcttt tgataaggat cttcgtggga caacaggtgg
     1741 gaaattaagc attacagatg cagacggtgt agagaaaaaa gttctgatcg aacatgaagt
     1801 tcaaaatggc aaagatatca aattgacaat cgatgcgaag gcacaaaaaa cagcttttga
     1861 cagtctagga ggaaaagctg gatcaactgt tgcgacaacg ccaaaaaccg gtgatcttct
     1921 tgcgcttgct agctctccaa gctatgatcc aaacaaaatg acaaacggga tctcacaaga
     1981 agattacaaa gcttatgaag aaaatcctga acatccattc atcagccgat ttgcgacagg
     2041 ttatgctcct ggatctacgt ttaaaataat tacagcagca atcggtctcg acaacggcac
     2101 tatcgatcca aatgaagtgt tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg
     2161 gggatctttt caaacaacgc gtgtcagtag tgatgtatca caagtagact taaaaactgc
     2221 tttgatctat tccgataata tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa
     2281 atttcgtaca ggcttagata aattcatttt tggtgaagac cttgatttgc caattagtat
     2341 gaatccagca caaatttcta atgaagatag ctttaactca gatatcttgc tagctgatac
     2401 tggatatgga cagggcgaac ttctaattaa tcctatccag caagcagcaa tgtattctgt
     2461 ttttaccaac aatggcacac ttgtctatcc taaattgatt gcagataaag agacaaaaga
     2521 taagaagaat gtcatcagcg aaacagcagt acaaacgatc gtgccagatc tgagagaagt
     2581 tgtgcaagat gtaaatggta cagcacattc tctttctgct ttagggattc cgttggcagc
     2641 gaaaactggt acagcggaaa tcaaagaaaa acaggatgta aaggggcaag agaacagttt
     2701 cttgtttgct ttcaatcttg ataaccaagg ttatatgatg gtcagcatgt tggaaaataa
     2761 agaagatgat gattcagcaa ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa
     2821 ttatcaataa aaaattgctt tttaagtaca gtaaaaggac tgtggcgtta tcgtcacagt
     2881 ccttttttca aataaacggt tttcacgatg tctctttcac aacttctttt tgatttactc
     2941 acacttcata aacttttttt gtaaaatatt tgagaacaag tggagaaata agtgtactta
     3001 ataaaatcac gataactaaa ggtgaatagt actgtggttc gatcaattga ctttgctgtc
     3061 caatttgtaa aatgatcaaa gccatttctc cacgcgaaat cataccagca ccgaccatca
     3121 atgaactatg ttgagaaaaa ccggcaatct gagcaccaaa ataacctcca agcaatttgg
     3181 tcaaaatcgc tacaagcgtc aatatagaaa taaagagtag ttgttcacta aagtcagaaa
     3241 aatcaacttc taatcctaca ctgacaaaaa atactggtat aaatactgca tatcctaaag
     3301 cctctacatt gtggtatact tcttctttaa cctttgtctg actgacagct atcccagcaa
     3361 aaaaagcacc aatgacagag ctaagcccaa caagatccgc tacgtaagac attcctaaac
     3421 aaataactag cgatacgatg ataatagaag aatttgcgta cactttttct gcaaatgaaa
     3481 gtaggaaagg cgcaatccat ttgactaata agaaaataaa aacaaaataa attagctgtt
     3541 caatcaaaac aagcggtaaa gaagcattgc tagaagcttc ccccgttaga aaagataggc
     3601 tgaaacttag aactaaaacg actaaaatgt catctaccac agaagcaccc aaaattgttg
     3661 atccttcttt tgtattcact acgtttagtt cttttaatac ttctactgaa atactgacag
     3721 aagttgccgc aagaataatc ccaaagaaaa tcgcttcatt tcccgaaact tggaatgctt
     3781 tgcctgtcaa ccagccaaaa acaaccggga agatgatccc aagcaaagca acgaacatac
     3841 caggtcggaa atattttttc aaaagagata aatcactttc cagaccagct aaaaacatta
     3901 ataagatcac accaatctcc gaaaagtcat ggactagaat atcaggtttg acccaattca
     3961 atccagcttg gcctagcaaa acaccaacaa gtaattgtcc aatcactgct gggataccta
     4021 atctccgcga gatatgtgag ccaatcgttg ttgaaatcaa tatcaggcat aaaataccaa
     4081 taaattccat tctcatcatt cctttgtata aaatatagcc cgaacttagc ataacataca
     4141 ttattgaaaa aataggaaaa tatactcaca aaaaaaccaa caatccaaaa gatttttttg
     4201 gattgttggt ttttatacgg tctattttaa taactaaaaa tgattaacgt atagtcattt
     4261 ttcttattct gatgaaattg ataatctcct ggatgcccat gaaaatcaaa ataaagccga
     4321 atatttggaa tagaagcatc aaacttttga atggattgaa caataagacg aatccgccaa
     4381 tcaacaacag aatactacta taaacactcc aacttttagc ttttgtattc atgctgaaga
     4441 acaactggaa caagctgttg atgataacag ctaatccaag aagaatcggc aggatagaaa
     4501 cgattgcagg tgcaaaagca tagacgatca gagctaagat caggaaaaaa agaccactga
     4561 acagaccgat actccaagaa cctgttttcc ttttaaaacg gtaagcctca aaaagattca
     4621 acaacccaag caaaacaaga taggcggtga tcagataacc gataaaatga aaaacagcag
     4681 aagggtttag cacaatcgca atcccagaaa taatataaac agcagctctt aataaggcgt
     4741 atttttgaat attttgttta atcgtctcaa acatataaga aaacctcctt tgacttattt
     4801 taacgtgaac tagtcatttt accaaattaa atgttacaaa atgagcgaag gagaagaata
     4861 tctctggaaa aatcaacttc tgaggatctt ttccattttt ttgccttttg ctagctcatc
     4921 gatcatttta tctaaataac gaatttcttt catcaagtta tcctcgatgt cttctactcg
     4981 tacaccacaa acaacacctg taatcaagga acgtgaagga ttggggacag gggcttccgc
     5041 aaaaaattgt tcgaaagtca cttgtttttg tagttgatat tctagttctg tttgggaata
     5101 acccgtcagc caacaaatga tttcatctac ttcctctttt atacgatctt ttctctccgc
     5161 ttttgctaca tacataggat aaacgcttgc aaaacttgtc gtaaaaatac gattttgcgc
     5221 cataatcagt cacctttctt tttttcaaat tatttactac ttatagtata taagaaaaag
     5281 agaagaaaaa gcgattattc aaaaaaacaa aaaggattgt gacaaaattc ttgtcaccat
     5341 ccttttttcc gctctctgac tttttacaat cttcatagct caagtgcgca tcagataatc
     5401 gaatgctcct aaagctgcag tagctcctga acccattgaa acaatgattt gtttgtatgg
     5461 actatctgta caatcaccag ctgcgaaaac gccggggata ttcgttgcgc catttttctc
     5521 ggtcactatt tcatttcttg gattcatttc gatcgttcca tctagccaat ccgtattagg
     5581 gagtaagccg actagaatga atactcctga aacagctagt gtatgcatct ccatcgtacg
     5641 gcgatcgata taggagagag attctacctg attcgtaccg ctgatttctt tcgtcgctgc
     5701 atgtttgata acagtgacat tcgcaagaga gactagttta tcctgtaaga tttgatctgc
     5761 ttttaattct ggtaagaatt ctagaacata tacatgtttt gctaacccag ccaaatcgat
     5821 tgctgcttca acaccagaat taccaccacc gataacagca atctcttttc ctttaaataa
     5881 agggccgtca cagtgtgggc aataagcaat tcctttattt ttaaactctt tttcgcctgg
     5941 tacattgata gaacgccagt gcgcacctgt cgcgatgatg gcggtttttg ctttaagtga
     6001 tataccgtta gctaagccaa tattaataaa atgttgctct ttatttaagg aaacggcctg
     6061 ttgattcatc ataatgtcga tagggtaact acgcacatga ctttctactt gctgcatcaa
     6121 ttttggacct tctgtgtaag aggtaccaat catattttca atccctagtg tttcaacgac
     6181 ttgtccgccg aaagaatcag cgacaagtcc tgtacggatt cctttacgtg ctgcatagat
     6241 agctgcacta ctagctgctg gtccaccgcc aattactaat acatcataag gttctttttc
     6301 aaacaactct tcttcaatca aagggccagt gattttttca acaatttgtt cgatcgtcat
     6361 gcgtccgcta ctaaattctt ctccatttaa aaacacagtt ggcactgcca tgattttttt
     6421 atcatcgatt tctttttgga acatcccacc ttcaatcatc gtatgagtaa tatttggatt
     6481 taatacagct aatatattca atgcctgtac aacgtcgggg caattatgac aagtcaagct
     6541 aacataactg tcaaaatgca atgtttgtgt tatttttttg attttaagac tcaaagattc
     6601 actgattttc ggtgcccgtc cactaatttg taacatagcc agtaaaaaag aggaaaattc
     6661 atgaccgagg gggataccag caaaaacgat tccactttct ttgccaactg gattgataga
     6721 aaaactaggt gtgcgcatcg aatctctttt ttcccaagag attttaggag acatttctgc
     6781 tatttccttc gtaaattcag ctagtttttg actattttca ctattatcca aactaacaga
     6841 aaaaacaata ggagattcaa tcagtgctaa atattgttcg agattttgtt tagtttcttt
     6901 gtctaacata tccaccacct aaatctttcc aacaagatca aaacttggtt tcaatgtttc
     6961 gccactttct ttccattttg ccggacaaac ttcacctggg tgggtacgta catattgagc
     7021 agcgcgaatc ttatcaatca gtgcactagc gtcacgaccg attccatctg cattgatttc
     7081 catagcttga acgattccgt ctggatcaat aataaatgtt cctcgctgag ctaatccttg
     7141 ctcttcatcc aaaacatcaa aaatacgaga aatctgatga gacggatcac cgatcatcac
     7201 gtattcgatt ttaccgatag catctgatgt atcatgccat gctttgtgag taaagtgggt
     7261 atcggtagag caagaaaaga cttcaacacc taaagatttc aatgtactgt attgttcttg
     7321 taagtcttct aattcagtag gacaaacaaa agtaaaatca gctggataga agcaaagaat
     7381 actccagttc cccataagat ctttttcact gacttctaaa aattctcctt gatgataagc
     7441 atgtgcatta aattcttcga tttttttccc aatcaatgac atagtaaaac ctccaataat
     7501 aattatttat ttataattat tgtaatttag ttggagttgc aaagtcaatg gatattttgt
     7561 tatttaataa agattactca aaaacgaagg ttaattataa aaatagaaga ggtcgcaaga
     7621 aaatgctact gctggaaata ctcgggataa cgttgagtaa cagtttcctg ttctttcagc
     7681 atcatgtgta agtgcaatcc cgtttgaaat cgagcttctt ctggtttttt atcaactaaa
     7741 gccttgagta aatcataatg ttgttccatg atcgtttccc atggaagccc ctgaatggtc
     7801 actcgtaacc agcggaaacg ttctaaatgg gtattatggt catctaacca gttccaaact
     7861 tcttttcttc ctgcaatttc gaaacaagct tcatgaaata aattatctgc acggaaaaaa
     7921 gcgcgttcgt catttatttt gactgctttt tcttgttctt ctaaaatcgt ttctagcact
     7981 attcgtgttt gtgtgtccaa tttggaacaa cattcaatca tgatttgctc ttccaactgt
     8041 tctcggacaa agcgagcatt ttctgcagaa acaagattaa ttttggaaac atacgtacca
     8101 ctttgaggta tcgtatacac gagttcttgc tgacgtagtt gaatcaatgc ttcgcgaatg
     8161 ggagtccggc cgatagcaag atgttcctcc agtgcctttt cagatatttt ccttccaggt
     8221 tctaagtcag catagataat ttgtttacga atcgcttgat aagcttgtga ttgcaagctg
     8281 cttttcatct ttattcctcc aaaatcattt ctatgaacgt agtataacag aagaggtctg
     8341 aaaaacaatc tggtatgtca cattaactag taaatttgta taaatagtaa aattttggtt
     8401 gacatagcga tttttattgt ataaaattca cttgaataca aaaactaata tgttagttta
     8461 ggaggggaaa aatgattgat ttaaacaact ggaggaactt tatccctgaa ttatctcaaa
     8521 aagaagtaga aatacctcat tttgaccgtg catcattaag agaaaatgga caaaaaaatc
     8581 ctgtctggat ccatttcggt ggcggaaatc tttatcgagg atttcatgca gaaatcgctc
     8641 aacgtttagc ggatctaggc gaattgtcca ctggtgtcat tgtttgtgaa acattcgatg
     8701 aagaaataat tgaaagggct taccaagatt ttgatgattt gattcttgaa gtggtcatgc
     8761 caagcagtgg agagttcgtc aaacgtatat tagctgcgac agctgacagt ctgtattgtc
     8821 atccagattg ttctgaacac tttgataaga tgattcgaca ctttgaaaat cctagtttac
     8881 aattagttac ttttgctatt actgaaaaag gttatcgatt gaaagataca aataataaat
     8941 atataggacc gattgcagtc gatataaaaa atggtccaaa gaagccatca catacaatga
     9001 gtattcttgc tgcgctactt tataaacgtt ttttatctgg agcttggccg attgcacttg
     9061 tctctacaga taatttttca caaaacggta gtcaattacg tacttctttg ctaacgatcg
     9121 caaaagaatg gcaaaaggaa ggttatgtac aaaatacatt tatccaatac ttatcgaatg
     9181 aagaaattgt tgcgtttcca tggacgatga ttgatcgtat caccccgaat ccttccttgt
     9241 cagttagcaa aatgttggca aaagagggct tttcaaatat gtcgattttc caaaccgcaa
     9301 aaggaaataa tattgctcca tttgtcaata cagaggacat ccattattta atcatggaag
     9361 atcattttcc caatggacgt ccgaaattcg aaaaagctgg ggtattcttg actgatcgtg
     9421 caacggtaga taaagcagat acgatgaagg tgacgacttg ccttaatcct ttgcatacgt
     9481 ctttagctat tatgggttgt ttactggggt ataggagcat agctgaggag atgaaagatc
     9541 cagatttatc gctgctagtt tctgaaatcg ggtacaatga aggcctccca gttgtggagt
     9601 ctccaggtat cattgatcca aagatctttc tggaagaagt aattactgtg cgcttttcta
     9661 atccaaatat tcctgatatg cctcagcgaa tcgccacgga tacctctcaa aaggtggcga
     9721 ttcgttatgg tgagacgatt aaaaaatata tggaaataga agttaaagac ccgcagacac
     9781 ttgtattcat accattgact ctcgctgctt ggcttcgata ccttattggc attgatgatc
     9841 aaggaaaacc attcatcccg agtccagacc cactattgac agactttcag cataagcttt
     9901 ccgatattca actgggtacg caagatacat cacatattca tgagaaaatg gctcctattt
     9961 tgtcaaataa agaaattttt ggtgtagatc tttaccaagc agagcttggc agaaaaatag
    10021 aagaaatgtt catcaagatg gtagcaggac cgaatgccgt acgaaaaaca ttacaggatt
    10081 acttacaaga acatggagga aatttatcat ggaaatgact tttagatggt acggatcaca
    10141 ggaagaaaag attaccttag atgaaatcag acagattcct gcggtcaaag ggattgtagg
    10201 cacattgatg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
    10261 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcccagggag
    10321 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
    10381 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
    10441 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
    10501 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
    10561 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
    10621 atcgaacatt ccagcaatga atttgaattg ccgggatggg aacctgagcg cttagctcat
    10681 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
    10741 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
    10801 catccagatg atccgccata ctcgattttt ggattacctc gaatcgtaaa gaatcgagaa
    10861 gatttagatt ggttgtgccg catagtagac agcccttata acggaatcac tctttgtaca
    10921 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgtcaa
    10981 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
    11041 tatgaggcac cgcataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
    11101 atgtatgaca acggatttaa cgaatacatc cgtcctgatc atggaagaat gatctggaat
    11161 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
    11221 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
    11281 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
    11341 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
    11401 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
    11461 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
    11521 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
    11581 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
    11641 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
    11701 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
    11761 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
    11821 gtttggactt ctttcaacac gctatgttct tgataaagca tgaaagtagg aaggggcgta
    11881 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
    11941 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaaa ggactccacc
    12001 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
    12061 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
    12121 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gggatcaata
    12181 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
    12241 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
    12301 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
    12361 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
    12421 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
    12481 atagacaaac cgtacaatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
    12541 gaggcactat agtttgtttg ctgacttaaa ctgataatcg aatttgtctg acagctgaac
    12601 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
    12661 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
    12721 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
    12781 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
    12841 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagaga
    12901 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
    12961 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
    13021 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
    13081 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
    13141 agacagtcca cacagaggag atagaaacag ccaaatggac agaaaccaag tgggccaacg
    13201 tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa
    13261 gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcaggagac ttagcaccta
    13321 tactacaatc agctgttaca tttgagaatg ttgatcccgc tttgtcgatt ggctatgacg
    13381 agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc
    13441 aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc
    13501 atcatcattt aacgccgtat agtcagcggc agacaattga gcaagattca gctatcgact
    13561 caggaggaga tcttgttgaa gaaccaacag ataaaccaga aaatgaaaat aagccagaag
    13621 taccgcctac agaaaatcca gacggagaac agaaaccgga aatagaacct ggagaagaac
    13681 ccgatacaga aactcaacca gagccagaca atgaatcaaa acctgaaata acccctggag
    13741 aaaaaccaga tgtagaccca gaagaaaaac cagatgtgac tccagaacca gatacggatt
    13801 ctggaaacca aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag
    13861 aaaaaccaga ggtagcccca gaagaaaaac cagatgtaac tccagaacca gatacggatt
    13921 ctggaaacca aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag
    13981 aaaaaccaga ggtagatcca gaagaaaaac cagatgtgac tccagaacca gatacggacg
    14041 caagagacca aggaatacca gaaaaaataa acaaaaaaac aattcaagag gatggaaaaa
    14101 aagagagtaa aaaatcaaat ctagcaattc taaaaataaa tgaagagcag ctgaataaaa
    14161 aatcacgaat atttgatagc gcccaatcag ctgaaacctt aaaaagtagc aaggatacaa
    14221 cctttgctgt cctgaaacga aaaataaaca gctaccaaaa tccggagagt cacaaaacaa
    14281 agttatactg tggagcggaa tcattctatt aagtatagca acaatgcttt ctgcaaaaag
    14341 atttaaacaa aacagatctc tctaacattt gaataatgtc tgtgaacgca tttttcaaat
    14401 aaaaagaaat atccagaaaa tgaccccaaa ataccagatt gaaatctgat atttggggtc
    14461 gttcatactt tatctttctt catatttatt ttaagggata tctgtttctt ttggaagatg
    14521 gttcgggatc tttttcactt tcaatacatc taaaaagaag gtaaagatcg gaatgccgac
    14581 aatcaagccc caaacgccaa agaaccgttc gctaacaagt aagaccacaa atgtataaaa
    14641 aatcggcagt tctgttttgc tggacatcaa cttcggattc agcacataag actcaattag
    14701 atggacaatc aaaatcgtaa tcaggatata aacaacatct cggatgcctc cttgggaata
    14761 agctatgaaa gatagaggaa tacaagaaat gataacacct gctactggga tcagacttaa
    14821 aatgaaaatc atgatcgcta aactcagcag ctgatgaaag ccaaatacag ccaaggcgat
    14881 tgtagtgagc aacgtattta aaagagcgac aacaaattga gcttctaata ccaaaccaaa
    14941 tgtattgaca aacttatcgg caaaatagta aatatcttga aagaaccaag aaaattcact
    15001 ttttaaaaac aatcttgaga atacaattgt ttttttcttt tcaatcataa aaaagaaact
    15061 taagatgaat gacatagcaa acgccactgc catttttcct atatcctgta aatagccaag
    15121 cgcaatcgaa gcgccatttt gaagctgttc aaaaaaatcg tttcgctcaa tataagtatg
    15181 gatatattgt aaaacaggat ctgcatcttt tggctgatgc tcgtaaaaat taataacgga
    15241 ctggatcatg tcaaatgttt gatgaataag catcgggata tactttgtga atgccagata
    15301 gataaggtaa acgatcagtg tataaatcat gatagctaaa acacctgtag ggattcggac
    15361 aaatcgctga atgatttgaa caagatgaat catcaaataa gtaaagataa atgtgagcaa
    15421 aatggttgta atcatacttc taactaagaa aagaacaaaa acgacaaata ataaaacagt
    15481 gagacggcgt attttttcat tcgccaaaaa acgctgataa acggtcaaat cgacaacctc
    15541 caaataacaa aaaataaaca cgttattcat tatatgatgg aatatgaaaa taagaaagag
    15601 caaaaagaaa aaccgctaaa tgacgttatc ttatgagttg gttttcctca taaaatcgtt
    15661 catctagcag tttcagccct ttttctggtc ttgatgaaaa ttatctagca tttttcgggt
    15721 cagatgttgc agtgtatcca attcttcctg ttcaagtgga agataagact tgatcttttg
    15781 tggaatacct tctgcttgtt tttgcaacgc agcaccttga gcagtcagcg taacatttac
    15841 gattctttca tcacccgttt cacgctgacg attaatataa tttttttctg ctaacttttt
    15901 tagtaaggga gtcagcgtac cagaatccag acacaaatca tttcctagct ctttcactga
    15961 agtctttttc ttttcccata aaactaacat cacaagatac tgggtatagg taagattcag
    16021 cggcttgagg aggggagtgt agtgtctaat gatttcttta gaggcggcat ataaaggaaa
    16081 acatacttga ttaactaaat atagatcttc catttttcca cctccgctgt tttactatag
    16141 tgtatcatag cggatttaca caaaaaagtc tattcactaa ctgctttcag agtaacttct
    16201 atatttccag ctgtagcttt tgaatacgga cacactttat gcgctgcctc taataattct
    16261 tgcgtttctt ccagagaaag accttcgata gatccttcaa tctctacacc taagacaaag
    16321 ctcgtttcac tttctgaata aagggaaact tgtgcactga cagtagaagc cccagagata
    16381 tttttttgct gtttgatcaa ttctaatgca ctgttaaaac atgcactgaa accagctgca
    16441 aacaattgtt cagggttggt tgcattttcg acgcgtttac caggagctgc aatattcaat
    16501 tcgaatgtac gatcgggatt atgcacttct ccagagcgtc cgcctgtgtt gatcattttc
    16561 gtagaataca tttttttcat aaaattacca cctttaatta tattttatac aattgaattt
    16621 tacacaatat aattgtggaa agcaaagaaa tggattggca taagataaat aagagaaagc
    16681 acatgaaaca gacgcgcttt ctcgaatagc aaacatatac aaaaaatatc agagacaaaa
    16741 aataatctca tcataaacat tacatatatt tctcaaaaag ttgttttctt tcttcaaaaa
    16801 ttacttcacg atggacctga tgaagcatcc ataaataacc aaaaggatct ctgaacattg
    16861 agttagtcac accaaagtcc atcatttctg tcatttcttg aatctctaca cagccatttc
    16921 gtaatgcttt attatgtgtg tcctcgatat cagagacgat gatattgaac cagatcgtat
    16981 ttggatgttc agcagtagga gcagtcaaac caagagacgt gttttcatcc agcatatgga
    17041 atctcgtacc atacaaggta aaaatgactt cattattgcc tctagggtaa tctgttgcct
    17101 caatcagttg tatttcatca aagattgttt gatacagttc tagtgctttc aaactatcat
    17161 ttaatacaaa atcgatttcc gttcctttat gcataaagat ctcctctcct tttcagtcga
    17221 acagttacta gattctttta ttataccagc taaagaaaac gcattcaaaa aaatagcgga
    17281 tttacttacc aactcattcg attggatgat aagtaaatcc gcgtcccatt acttctgcta
    17341 cgctgctgac aatgacaaag gcacttggat cgatttcttc tatcaatggt ttcattcggg
    17401 aaaattcatg atcagtcaca acgatcaata aaacctcttt tgactttcga ttatacccgc
    17461 cttttgcctc tagcagggta aggccacgtc caatctcagc acttaagcga aggcggatgt
    17521 cttccaaata atgttcactc atgaccataa cagatttctt tcgattgata cctgtttcga
    17581 tataagtcat gacaattgaa gtgattgcaa tagataaaat agcataaaga aactcttcta
    17641 ttccaaaaat aaacagatta agagaaacaa cgaatgcgtc tgtaaaaaat aagccaatcg
    17701 aagtattcaa gtggaaatat tttttaaaaa tgaggggagg gatcgtcgta ccgccgctag
    17761 aagagttgtt tttatataaa atcgcaactc ctaaagcaaa aatcgcactt ccaaaaatga
    17821 cagacaacaa ccgatctgaa gtaatcattg tttctggaac tattgcaatg attaaaggaa
    17881 aaaccaagct tccgaaaagt actttaaaaa agggtttttt acctaaaaat agaagagcta
    17941 gtaccaacat aacgatattc aacactaaaa taactatagc tcggtcaaag cctaaagcag
    18001 cttccagtaa tattcctaga ccgctgacac ctccagcagc aatatgatga ggccctaaaa
    18061 acatattgat agcgactcct aatatgaaga gggcaaaaat aatcttaatg tatgtccata
    18121 taaattgtct cgtttctttc atttattcac ttccttaatt cattaataat actcttatct
    18181 tattataact tacttttcac cttaagttaa ctttaattaa taaaaaaatt aggctagtct
    18241 aaattattat gtttcattct tctagaatat acttctacgc caaaaactgt taaaaaactt
    18301 ttactttaga aaattggaga aattgatcgc ttaagataaa ttcaggacac taaatagtac
    18361 aggaagtaga ctaaaacttt taaaagtttt aagtctatct atttggcaag tttaaaaaat
    18421 aaaaattcgg tatcaaaaaa ctgttaccga taacattttt gaaagcgttg tcttttgctt
    18481 ctctatcact tatagtgatg acgtcatatt aaaacacaag gaggaaatca atggatacag
    18541 ctgcaattta tcatcgaccg gaaagtgaat atgcatttct atatacgaat acaagatttc
    18601 gtattcggct tcgtactagg aaaaatgata tcaaaaaagt atatgtgcta tgtggagatc
    18661 catataccat aacaactgaa aaatggtatc aaaaacaacg accaatgaaa aaatgtctaa
    18721 gtaccaatgt tcatgattat tgggaaattg aagtgacgag cgaaacaagt cgcttacagt
    18781 atgcgttcca tgttgttggg gaagataaca cggactgttt ttacggtgat caaggaattt
    18841 ttccttatcg agaaaatgtg attactgaac caaattttta ttttcgaata ccttattttc
    18901 atcaaatcga tcgattcact attccagaat gggtaaaaga aacagtatgg tatcaaattt
    18961 ttcccgaaag atttgcaaac ggggacaaag caaatgaccc cgaacacact ttaccttggg
    19021 gaagtaaaga tccagaccga gaagactttt tcgggggaga tttgcaaggt gtgatggatc
    19081 atttggatta cttgacggac cttggcatca atggcatcta tttttgtccc atatttaaag
    19141 ccacatccaa tcataaatac gatacgattg attattatga ggtggatccc gcttttggcg
    19201 ataaaaaact tttaaaaaac ttgattgatg aagcacataa aagagggata cgtatcatgt
    19261 tcgatgctgt attcaatcat atgggtgtcc attcaccaca gtggcaagac gttctcaaaa
    19321 acggagaaaa atcgatctat aaagactggt tccacatccg ttttttccca gtagattctt
    19381 atcagatgac tgacttacca gaaactgctg aaaatatccc atatgatact tttgctttta
    19441 ctccattcat gccgaaacta aatacagcaa atccagaagt gcaaaaatat ttgctggata
    19501 tcgcaactta ttggataaaa gaattcgata ttgatggatg gcgtcttgat gttgctaatg
    19561 aagtcgatca tcatttttgg aaaaaattca gagaagcagt tacagaaatc aaaccagaca
    19621 tctatatcct tggagagatt tggcattcct ctcaagcttg gctgcaaggg gatgaattcc
    19681 atgcagtgat gaattatgca tttacagatt cgattaaaga ttattttgca aaaaagaaaa
    19741 tcacagcttc acaaatggtc agtggaatga atcatcagca aatgctttac agagatcagg
    19801 tgaacgaagg aacattcaac ttattagatt ctcatgatac tgccaggatc ttgacccttt
    19861 gccaaggtaa caaagaattg atgaagtctg tgatggcctt tatgtttctt caaaaagggt
    19921 ctccttgtat ctattacggg acagaaatag ggatgactgg tgaagatgac ccggattgcc
    19981 gcaaatgtat gatatgggag aaggaggaac aagatctaga gctattcggt tttataaaag
    20041 aacttgtttc tctaaggaaa cagttgtcta agattatttc tgaaggttct actcagtggc
    20101 taatagtaaa tgatcgtgaa gataagctct atttcacacg gaaactagag ggacagatca
    20161 tctatgttta ctttaatcag tcaaaagaac cttgggtagt agaacaggaa aatgaggtga
    20221 ttcttagtca aaattgtcag ttgttagaag acgggaaagc agagatacga caatacggat
    20281 ttttgatcat gtgttgaaaa ctgttaccgg taacaacaag aaagcgtttg caaatgaaga
    20341 tttgtgccca ttataataag ggtgtaacag cacgttgaat acaaaggagg aaatgaagat
    20401 gaaaaaaaga agtgtgaaac agttgggttt agggttaatc tctttaggaa tcttaggcgg
    20461 attagcggct tgtggcaatg gtaactcttc agcagaaggg gaagccgatg aaggaaaaac
    20521 gttaacagtt tccgttgatt ctggatataa agattatata aacgaaataa aaaacacttt
    20581 cgaaaaagaa aacgatgtga aaatcaagct agtagaaaag gacatgttcg atcagttgga
    20641 atcattagcg ttagatgggc ctgctggaaa gggacctgac gttatgatgg cagcatatga
    20701 ccggatcggt gcgttaggac agcaaggaca tctggcggaa gtaaaactgg gaaatgaagc
    20761 agcttacgat gaaacagaca aagcccaagt gacttatgat ggaaaaatct acggagaacc
    20821 tgcagtaatc gagacccttg ttttgtacta taacaaagat ttagtcgata cagcaccatc
    20881 aacgtttaaa gatttggaga atctatccaa agacagtcgt tttgcttttg agtcagaagc
    20941 aggaaaaaat acaggatttt tagctaaatg gactgacttt tattattcct atggtttaat
    21001 tgcaggttac ggtggctatg tatttggtga tgatggcaca aatccttcag atatcggttt
    21061 gaataatgca ggtgctgtag aaggaatctc ttatgcaaca gattggttca aaaatgtttg
    21121 gccaaaggga atgcaagaca taaaaagtgc aggagacttc gccagccagc aattcatgtc
    21181 caataaaact gcagcaatca ttgacggtcc atggcaagcg caaacataca aagaaaacaa
    21241 tatcaattat ggtgtagcca aaattcctac tctaaataat ggacagcctt atcaaccgtt
    21301 tggcggagga aaaggatggg tagtcagcaa ttatgctaaa aacaaagacc tctctcaaaa
    21361 atggcttgat tatgtaacga atcaggaaaa tcaagaaaaa ttctttgaaa tggtaaatga
    21421 aatccctgca aaccaacaag ctagggaaac agcaaaggct aaaaacgatg aattaacaac
    21481 agctgtgatt gaacaatatg aaacagctca agcgatgcca aacatcccag aaatgggcga
    21541 agtttggaca ggtgcagaaa acttgatgtt cgatgcagca tcaggttcaa aaacaccaaa
    21601 agaatccgca gatgaagcag taaaaacaat cagtgaagcc atagaacaaa aatactgatt
    21661 aaaatgataa ccgtccattt ttataaaaaa tggaacaggt gcttcagata aaagtggcaa
    21721 gagcgagatg gaaaattatc ttgctcttgt tgccattatc ttaaaagcag tttgcggttt
    21781 agcaagttta gagggggcat tcagatgcag caagataaaa caagcaatat caacgaaaaa
    21841 aaaatcacgc tttgttcact tattccaggg atggggcagt ttgtaaatgg ccaagctttt
    21901 aaaggtatcg tttttttcgc catattcgtc ctattcattg gtcagatgct tctgggcgga
    21961 tttcatgcat tggtaggatt ggttactttg ggaagtatac caatggaaga tcattcatta
    22021 tttattttga ttgaaggcac tttgcaattc atcattacaa tcattttctt gattttttac
    22081 ggtttgaatc ttttagacgc ttatcgagta gcgaagatgc gtcaagaggg caaaaaaatc
    22141 aatcggacga tgaaggaagt catacataat ataggtgatg aaggttttcc ttatctattg
    22201 acacttcccg cttatcttct catgattttt acgatcattt tcccagtcct agttacattg
    22261 ttcactgcat ttactaatta tgacttcaat catattccac cagccagttt aattgattgg
    22321 gtaggggcta aaaacttttt tagtatcttc tttttaagct catatcgtaa tacttttttt
    22381 gcagtctttt cgtggaccgt gatttggact ttatgtgcaa caactttgca aatcaccctt
    22441 ggtattctca cagcggttgt tgctcaccag aaatttatta aattcaaacg tattttcggt
    22501 atcattttct tattaccatg ggcggtccca gcttttatta ctatcatgag tttttcgaat
    22561 atattcaacg atagcgtagg tgccatcaat actcaagtga ttcctttcat aaaccagctt
    22621 ccttttgtag atatcagtgc aatctcttgg aaaacggacc cgaactggac gaaagtagca
    22681 attatcttag tgcagggctg gttaggattt ccctatatct atgtgatggt tacaggaatc
    22741 ttacaatcga tttcagaaga cttatatgaa gcagcaaaaa tcgacggagc aaatgcatgg
    22801 cagcgctttt caaacatcac gcttcctgct gtcttcctag tggcagcgcc aacatttatt
    22861 acacagtata cggggaattt caataatttc tccatgatct atctgtttaa caatggcggt
    22921 cccggaagcg taggcggagg agctggttct actgatattc ttatttcctg gatctataaa
    22981 cttacgactg gaacttctcc gcagtattct atggcttcag caatcacgtt gattatctcc
    23041 gtcatcgtta tctgtgtgtc attgctggta tttaaaaaga caaatgcgtt caatatgggg
    23101 gattaacaat gaaaaaattt cattcgagtg ctcgattcca aaaacgactt tctcactttt
    23161 ttacttactt ttttctttta atactgtcag tcattatcat ctatccgctt ttgatcacag
    23221 caagtactgc cttcaagtca ggaaatattg cagcttttag tttagatctt tcaagtgact
    23281 ggactttagg aaactttact cggctattca gtgaaacact ttacgggact tggtataaaa
    23341 atacattgat catttctgtc ctaacaatga tcgtacaagt gactgtagta actttggcgg
    23401 gctatactta tagtcgctat cgttttatcg gccgcaaatc aagtttgatg ttctttttag
    23461 tgattcagat ggtaccgact atggcagcat tgactgcctt ttatgtcatg gctttcttat
    23521 tagaagcgct cgataagtac tggttcttga caatgatcta tataggcggt ggaattccaa
    23581 tgaatacttg gctgatgaaa ggatactttg acactgtacc aatagattta gatgaatctg
    23641 ctaaacttga tggggcaggg catttcagga tttttttcca gattgtatta ccgcttgtca
    23701 aaccgatgct cgccgttcaa gcattatggg cttttatggg accttttggg gatttcatgt
    23761 tagctaagtt cctgcttcga acacctgaaa atcagactgt tgctgtcggc ttacaaacat
    23821 ttatttctga tgtgacaaac caaagagtct ctctttttgc tgctggggca attttgattg
    23881 cactaccgat ttgtctatta ttcttctttt tacaaaagaa ctttgttacc ggcctgacat
    23941 ctggcggaac aaaaggatag gagcagagta tcatgcaaac atttccgtta aactattttt
    24001 ccgcattacg tacgccaact caagtgtttg cgggacgcaa actattatca tggcctaaat
    24061 ttttcttgat atttgtattt ttagtttcct tgatggtcat gcctgtgact cttttttatg
    24121 ccaatcagat ccaagcgatt ccattagaac aatttctatc cgttcattcg ctgatcgatg
    24181 aacaaggaac ccaaaaattt tctgaacttg aattatctga aactgggtta caagcatcgc
    24241 aacaaacgat agctgtgacc cctgaaattt tggtaggtgt gtctttatct gagaaacaac
    24301 aatccgatca tggaactttt attgattttg agaaggaaca atgggtgatc caacaaaagg
    24361 ataaatctgg tatacgccgc tatacgatga attatagccc atctttccaa ccagattctg
    24421 tacgtacgcc cgaagatttc caacgatttt tagaaagaga attttatgcc agtaatcgtc
    24481 caacaatcat tttatcttat agtttaagtc ttggtcttgt cctatttgtc atgacgagtc
    24541 tcattttatt cggggcttcc tttttcttgt ggatgacaag aaaaagccaa ctttcatcca
    24601 ttcatacatt caaagagagt gcaaatttga tgctgaatgt tatgggaata ggttccatgg
    24661 cagcagcagt tgtagggttt atccactttg actttatctt gatgctaagt gtccaaacaa
    24721 cgataactgt cttgctgctt ttatgggttt ttgtcaaaac aagattcaaa gataagcgtg
    24781 tcgaatgaca agtttaagcg atttatgcga caatgaatgt gaaaaaaatg aaaaataaag
    24841 tagtttttga aaaatttctg aaaaaattaa acttcttagt tgattctttt cttttttagt
    24901 attaacgtaa tagggtagtt tttttaagga ggaaaatcat gttagagaaa actttataca
    24961 atcaattatt ttcacgttct ttctcgctcc ctgtcgaagt aaattattgg gacggtacaa
    25021 cgaaacaata cggagatacg gacactcctc cccaaatcaa aatcaatatc catgaagaaa
    25081 tcccaatgaa agagattaca agtaacgctt ctttggcact aggagaagcc tatatggatc
    25141 atcgaatcga gatcgaggga agtatccaag cattgatcaa cgatgcttat cagcaagcaa
    25201 acagcttttt acgaggaaaa gattatctaa aatggctgcc aaagaaagaa aaacatacga
    25261 aacaaaaaaa caaagaagat atccatgcgc attatgactt aggtaacgac ttttataaac
    25321 tttggctaga tccaactttg acttattctt gtgcttattt caatacacct gaagatacgt
    25381 tggaacaagc acaaatcaat aaagtccatc atatcttaga caaactcttt atcaaagaag
    25441 gcgaaacgtt gcttgatatc ggttgtggct ggggaacatt gatgttcact gctaccaaag
    25501 agtataatgt caaggcaact gggatcacac taagtgaaga acaatacgat ttgattacca
    25561 aaagaataaa agaagaacat ttagaagata aatgccgtgt attattaatg gattaccgcg
    25621 aactaaaagg cgaaacgttt gatcatatca caagtgtagg tatgttcgaa catgtaggtt
    25681 ctgaaaatct tgaaggttat ttcaaagtgg taaaagattt attgaaacca aaaggaacag
    25741 ctttgatcca tggaatcagc cgacaacaag gcggtgcgac aaatgcttgg atcaataaat
    25801 atattttccc tggcggttat gtccctggtg ttactgaatt agttgataat attacaaaaa
    25861 acgatttgca actgattgat ttggaaagct tacgtcgtga ctaccaattg acattagaac
    25921 attggacaaa gaattttcat caagtccaag atcaagtcac gaaagaaatg ggtgattcct
    25981 tctatcgaat gtgggatcta tatttacaag cttgtgcagc ttcatttgaa tcaagtaata
    26041 tcgatgtgat tcaatatttg cttgttcaac ctggaaacaa tgacatccca atgcatcgtg
    26101 cataattgag gcaaaagtac tgtttaaaga ctatttaaaa agaagctaag ttttatgatg
    26161 ctccagaagt gaactttttt gtctaacttc taggattgtc tctaaagctt agtttctttt
    26221 tctttatatt ttcataataa aagtttggtt ctttaatttt taaacatgac agaaaatttt
    26281 ttcttcgtta tacttgtaat agaatactat tttataacaa aggggatcga atcaatgaaa
    26341 aaaactgtta tttatactag tttatttgca gctttgtttt tagctgcttg tcaatcaaat
    26401 accaatcaag gcacatctgg gaatacgtct tccagtagcg aacagcgctc ttcacaggta
    26461 tcggactcct caacaaaagc aagcacatct tcgtctagcc agacaaaaag ttctagtgaa
    26521 gcaagtactc agtcagatat caaagtgtct gcagctcgtg cgatcgaatt gttccaagat
    26581 aaatacccag atactgcaat tactagtttg gaattagata gtgattgggg atcttacttc
    26641 tacaagatag aaggtgtcga tgatcaaaaa gaatacgaag caagaattga tgctcaaaat
    26701 gaaaaaatgg agacagagaa tccagaaact ctagatcgag atgaacaaaa tggtcaaaag
    26761 aaaaaagaag atggtctgga tgttgataaa ttgatcacga ttgaggaagc agcaaaaatc
    26821 gccgtcgaca aagtaggaag cggtacggca acagattggg atttggataa agatttaggt
    26881 actacttatt gggaagtcaa agtaaaagac ggcaatcaaa gtacatcagt caagatcaat
    26941 agccaaacag gcgaagtact gtcaactgaa caagatgatt aagaatttct tcggaaaaca
    27001 agagttgatt tttcttttag tcatgataca ctagacaaaa taacttgttt gaaggagcga
    27061 tttacttgag tttagaaacg acctttgcca atcatatctt tgccaatcct ttgatgaatg
    27121 catctggtgt ccactgcatg actacccagg aattagatga actggctcat tcagaagcag
    27181 gagcatttat cactaagagc tgtacgatca atgaacgaaa aggaaatccc gaaccacgct
    27241 attttgatgt tcctttaggg agtatcaatt ctatgggact tccaaactta ggtttttctt
    27301 attatctaga gtatgctctt gcgtatgaaa aagtgcagga aaaccaaaat caaccgttat
    27361 ttttttccat tgccgggatg agtgtccagg aaaacttgga aatgcttgag aaaatagaaa
    27421 aaagtgggtt taatgggatc actgaattaa atttatcttg tcccaatgtg cctggaaaac
    27481 cacaactagc ttacgatttt gaggccactt atgaaacact gaaagaagta ttcagcatct
    27541 tttctaagcc gctaggaatc aaattgcctc cttattttga ttttgctcat tttgatcaga
    27601 tggctgatat cttgaatcag tttccattga cctatgtcaa tgctatcaac agtgtgggca
    27661 atggtttgta tatcgataca gaacaagaag cagtagtcat caaaccaaaa gaagggttcg
    27721 gcggcattgg cggagagtat atcaagccga ctgctttggc gaatgttcgc gctttttata
    27781 cacgtttgaa accagaaatc caaatcattg gtacaggtgg gattcggaca ggacaagatg
    27841 catttgaaca tctgctatgt ggcgcaagca tgttacagat tggtaccgag cttcataaag
    27901 aagggccgga aattttttct agaatcataa aagaattaac gcagattatg tcagaaaaag
    27961 gatatacgtc aattgacgaa tttaaaggca aattacgcac catttcatag caaagagacg
    28021 aaggtcatga ttgatcttcg tcttttttta taaaaaacac tcgtctgttc attcctgtgc
    28081 aagcgtactc attgtatgtt agacgaactt gtgggaaaat aacagaggca aaaaggagga
    28141 taatatgaat gatgcaacag aattagcaaa agagcttaaa gaaaaagaat ttagttttca
    28201 agaactagtt acggatatac agaaaaaaat aaagaagcaa aatcatgaat tgaatgcgtt
    28261 tgtcgcgctt gaaccattcg agagtaattc ccacgagcta ttgattcccc agtcagaaaa
    28321 agggccattg gctggtatcc catttccttt aaaaatgctc ggtcaagaaa aaaagggatg
    28381 gctagctact tcaggatctc gtttatttga aacacaccga gcttctcata catctaatta
    28441 tgtgcggcaa gcagaagcga taggtctagt tccttttgga caaaccaatg cgccagagtt
    28501 tggtttcaaa aatattacag acccagtcat ctatggccca gcaagaaacc cttggaacct
    28561 tgaatgtaca ccgggcggat caagtggagg agctgctgct gcagtttcat caggaatcgt
    28621 tcctttagct ggtgctagtg atggcggtgg ttctattaga atacccgctt cgttttgcgg
    28681 attgattggg ctgaaacctt cgcgagggac gatgccagtt ggtccatatg cttggagagg
    28741 atggcaagga gctgctattg atttcggctt gacagtttct atgagagaca cggaggcttt
    28801 attttatggg atgagaagta tccattccgg tgcgccatat caagtatcgc cagttgagtg
    28861 gcaaacacat ccaagaaaaa aacggttgaa gatagctttt tgtgttgatt caccgatcca
    28921 ctcacagatt agtgaagagg cagtacttgc tgtaaaaaaa gctgtagatt tccttggaaa
    28981 agaagggcat gatttgacag agattcctta cccactagat ggtcgtaaac tgatcacgtc
    29041 ttattatcag atgaatggcg cagaaacagc agcaatgatc cgctcgattg aaacaggcat
    29101 ccagcgagct gtcagaaaag aagaacttga acccatttct tggactttgt tccaatatgg
    29161 tcagaagctc tctgccgcta cttacatcga ttctctgcat gtatgggatg aagcagcaat
    29221 tgtcatggaa aacctacatc aagaatacga tctattcctt tcaccaacga ctgcatttac
    29281 cgcccctaaa atcagcgaag atttgcaaag tgatgcgata agagcacaga tggaacacat
    29341 cagtgattgt tctgaagtag aaggtttaga cgttgtttct tccatgtttg aggagagctt
    29401 gaagatcaca ccttatacac agttggctaa tctgactgga caaccagcaa tcagcttgcc
    29461 tactcatttg acaaaagaaa acatgcctct aggaatccaa tttatggctg ccagaggaag
    29521 agaagatttg ctatttcaaa tcggctatct atttgaagca aatcatcagt tccatttacc
    29581 aaatccatgg aaatagcaga aacaaggaga agaagaatga agaaaaaaag attgtttgct
    29641 ctaataacta gtgccttttt tattttaagc gcttgcggta cagctgccgc taatcaggaa
    29701 gataacagct ggagtaatgt agaaaaaaag aaagaattag tagtagcgac ttctggcaca
    29761 ctgtttcccg catcttatta taatgatcaa aatgaattag ttggttatga tgtagatttg
    29821 gcaaaagagg tggctaaacg gttaaatgtc aaaatctcct ttaaagaata caacgtggat
    29881 ggacaaatca cttctgtttc aaaaagagaa tcagattttg ctgcgaacga ttttggtatc
    29941 acaaaagaaa gagaaaaaaa attctccctt tcagagactt tcaaatattc atttgacagt
    30001 atgatcgtac gaaaaaaaga tgattcagga atccactcac ttgaagatct taaagggaaa
    30061 aaagcagctg gcgaacccaa tactagctat atgcgtttag cggaaaaaca cggagctaaa
    30121 ttagtcacat atgacaatgc cacgaatgat cagtatttaa gcgatgtagc aaatggtcga
    30181 acagatgtta ttctaaatga ttattatctg cagaaaatgg ccgtatctgc tttaccagat
    30241 atccctgtaa aaatactgga gaacgtttat ttcaatgaaa acgaaagtgg ttttttattg
    30301 aaagaaggaa atgatgctct gaaaaagaaa atcgatggcg tattaaaaga gatgaaagaa
    30361 gatgggacga caaagaaaat atctgaaaaa tattttcaaa ctgatgtttc agtcaaaccg
    30421 aaagaaacga tggctgagtc ggtgagtgag tgatgtactt tcctctaatt gatttttccc
    30481 tcatgtggac ttcacttcct ttcgtactgc aaggcttggt atacacatta gggattggct
    30541 tcgtgtcgtt tcttttagga aatctggtag ggttgctttt gacagtttta ggtcttttgg
    30601 attggctgcc tctgaatgta tttattcgtt tttatttatc gttctttaga ggcattcccg
    30661 cactagtcct gctgtttctg ttatattttg ggttacctta tcaattaagt gccttaacgg
    30721 cttcagtcat ctgctttacg attaccagca gtgcatttat tggtgaaatc tatcgaggat
    30781 cactagcagg cgtttcttca ggtcagtggg acgctggttt agcttctggt ttttctttct
    30841 ttggggtatt gaaatatatc gttttgccgc aagcttttcg aatctcagta ccggcactca
    30901 gtaacgtagc tatggatcta gtgaaaggta cttcgttagc agcaacaatc acagtgccag
    30961 aaatatttca aaaagcaaag atcataggcg gtcggacatt cgattatttt tctctctata
    31021 ttttagttgc actgatttat tgggtcattt gtatcgggat cgaaaaagtg caacagtatt
    31081 tagaaaaacg ttctatgcat cagtcaatct gaaaaataga aatggtgcta gcgtggctgg
    31141 caccatttct attttttgtt tcttgtatat tctgcctagt attttaaaac catttcatgt
    31201 aaaatgtaaa tcgaaaatat gttcggaggt agaaaacatg aagaccccgc taaccatcga
    31261 aatagaatcc tcgttatacc aatattgcat cgagcaaggc ggcttggttg tagaagaagt
    31321 cacgatgcct gatgaccaag gaattgtaga tacgcttagc tgcttgacaa aatttgatgg
    31381 aagccgagaa tggcgatgct atgagctgaa agtgacaaaa gcagattttc gctctacagc
    31441 caaactgtca tttgttggac attataatta ttttgtttta ccaagtaaac tatatgaaga
    31501 agtaaaaact gaaatcccta caggaatagg tgttttagtg tatcgtccgt acacggttga
    31561 agaggagatg cctgcctctg gtacatttct gattgcgaaa aaagcagcaa agaaagagct
    31621 tgctgtaccg gaagaagctt tgacaaaccg cttcatggct tctttattca gggaagtaag
    31681 aaaagcaaag caaatggagc gaggcgtaaa atattttcca acagatcaat tgtacaaaga
    31741 attgaaaaga cgttcagaaa atcaagatcc attttctaac gaacgatatt atgaacggtt
    31801 tttagaggaa atagctagtc aggcaatcaa tgagttgcaa gaagaagtta tcgcgcttca
    31861 gcatgattat gaatatttga ggcaacaaca gcagatccgt cggttgccta cagagccatt
    31921 ggagtgaaaa atatgtacta tccttattta agaggaaaac aatttgacct attagcatta
    31981 aaagaagcac tgtcgcgagg attgctctcc aataaaatcc aaccagtgat cgaaccagta
    32041 cgtgattccg ctacgcttaa aaatgtgatc gaattatttc aaaaaaagca ccatccaatc
    32101 gctgtgatcc aaaatcccca agtaggacaa ttcaaattat tcgatcagca tgtgcacagt
    32161 tggacagtca aagaaaatag ctctgtcgtc cctgcacaga tcttaacgcc agaaaatctc
    32221 agtgaagtac tggattcacc tccagcgttt cttgttttcg atggacagca ctacccaaaa
    32281 gatacagagg tgtggaaaca gttggcagga gttgattcga aatttttgat accagatact
    32341 agtcgctttc gcatctggct gcccgaaaac aaaattgtga tccgtgactc ttttcaaacg
    32401 agaaagcatg ttgaaagtta tgcagataaa acagatgact tttttagtga tgactatctt
    32461 tttttccacg cagacggata tattggattt tctgatttca cgatcgaagg tagccgttat
    32521 ttcgataaag ggttccctag ccgagcgctt gctatccatc taacgtatat tgatgcttac
    32581 ggcaatatac gcgtgaaaca ttttgtctct gacagtaacg actctgctaa agaccaagca
    32641 ttgaagtttc ttgaagcagg agataaaatg aaaaaatgga tcatgcgtca ccatcatcaa
    32701 ctcttgatta cttcaggaat gattgaatta ttggctcttt accagcaaca aaaattccct
    32761 ggactgggag tgttgaaaaa gtggtcactt atgcatcatt tggaacttat tagtcacctg
    32821 ctagatcatc caaaagattg gcttaaatat tattcaaaag ttcaggaact atatgaagtg
    32881 atccaatgat tagataatgt aaatgaaaaa ggaatagaaa agaggaaact ggatgtatat
    32941 tcggcaaaca aggaaagaag aagtagaaga agtggtagaa atcattaatg ccgcagcgga
    33001 tttcattgct tcgcaaggct ctcctcaatg gcaaaacggg cagaaaccaa caaaagaaac
    33061 gattttgaac gatattgaaa gaaaagaaag ctatgtatta atagtggaag atcaaattgc
    33121 tggaacagct gcattagtct caagcgtgga tcccgtttac acaaaaatag atggtagttg
    33181 gaaagaatca actggtcctt atttatcgat tcatcgtgtc gctttagcag aaagtactcg
    33241 aggaaaaaaa ctagcaaaac cgttcttaga acatttgttg acgattgcag ccagcaaaaa
    33301 tatccaagat atccgtatcg atacttatcc agctaatgaa ccgatggaaa agaccatcta
    33361 ttctgctgga ttcacttatc aagggatgat tgaatttcct tttgaacacg gcgaaagaaa
    33421 agcatatcaa ataatcatca attgactata aaatcatctg agcaaagata aagacattaa
    33481 cactactgaa aaaaacaatc atatgatagc caagaggttg taacagaagt ggccagcttc
    33541 aaaaaacaag aaagaatttc cgaaaatgct tctcaaattt ttgtggaatt ttagcgtatt
    33601 ttacgaaata gctgctttct gaaactccct tcatcgagag gtcgtgacat agcttttgtc
    33661 acgacctctc ggcttatcct acgagtatta ttcgatacat ttatatcaat aatccttttt
    33721 ttattttatc tgcagcttct tggaacgctc catcgtttga cacgactaaa agtgagggga
    33781 tagctttcaa acgtttcaac tgtgctaaat cttctacata aaccgccagt ctggctgaaa
    33841 tctgttcaga tgaatctcca cgttttttca ggcgcgattg caatgtttct ttagagatcg
    33901 taataaagat aggacggact accgtcccga aatactcata taaatgccaa aaaccatttg
    33961 ctgtcaaaac ggcgtaacaa tttcctactg tcagtttctt tttaatttct gctttactgg
    34021 aaccatattt atgttgagca tattggtccc actctgcaaa ctcatttccc ttgatcatct
    34081 gattgaatgt attgtcagaa acaaaataat aatcctcttg atctttttcc ccaggtcttg
    34141 gtgatcgatt cgtgtaagta atgatctttt gttccggctg aaaaatagct tctgcgagtg
    34201 aagtttttcc agaaccagaa ggaccgataa atacatagca ataattcatt gtcatttctc
    34261 ctttgatgat tgaacaagtg aaaaagcttt ggaaaatatt tccaaagctt ttaaaagtaa
    34321 ctttgctttt ttgtatatta taccagcaag ccagtcacaa tagcacttaa aaagctgact
    34381 aaagtcgctg ttagcaaaat tttcaatccg tgttttgcga caagatttcc tttttcttca
    34441 tttaatccct tcatcgcacc agaaatgatc ccgatagaag agaagttggc aaatgaaacg
    34501 aggaagacag atataattgc tgttgtacgc tcagtaaaat ggaaattacc atttgctaat
    34561 tctgtcatag caacaaattc atttgtaacc agtttagtcg ccatgatact accggcatcc
    34621 actgcttctt tccatgggat tccgctgatg aaggcaagag gggcaaagat gtaacctagg
    34681 atctcttgga aagtgatgcc gaatatcccg tggaagatag cattgatcat ggcaatcaaa
    34741 gcaacaaaac cgataagcat agcagcaacc gtgatcgcca catggaaacc atcaagaatg
    34801 tattctccca acatttggaa aaatgtctgt tctttgtttt cttcaatgat cagctcatct
    34861 tctttttctt ctaatacata gggatttaca attgaagcca gaatataacc gccaaataag
    34921 ttcaaaacaa gggcagtgat cacatattta gaatcgatca gtgccatata agatccgacg
    34981 atcgacatag aaactgtaga catagccgat acactcatgg tgaacaaacg tttctcagat
    35041 aggaagggta attccttttt tatggaaata aaaacttccg attgtcctaa aatagcagaa
    35101 gctactccat tataagattc caatttgccc atcccgttga ttttacttaa tcctagacca
    35161 acagctttca taaataatgg aagaatcttg atatagcgta gaataccgat aatagcagaa
    35221 ataaatacaa ttggcatcaa tacggaaaga aagaaaggtg cacttccttt atttgctaca
    35281 ccggcaaaaa caaagtcaac accttgccca gcaaaagcaa gaagatgatc aaacattttt
    35341 gccaatacag aaacaaccgc tgtaccaaaa gttgtacgta ataatacgaa accaagaaca
    35401 agctgtaaaa cgaacatcac aattagtggt ttcaatttga ttgctttttt gttactgctg
    35461 atcaaaaaag caagccctaa tgtgacaaat aaaccaataa tacctaataa ataactcaaa
    35521 taatcactcc tattccaaat tccgttttat tttacctgtt ttgcaaataa aatactaatc
    35581 tttagtgaaa ttttcattac ttttttagag tgaaacgcat tcattttcca ttagaaaata
    35641 aagcgaacaa aataaaaggt ttaaaaagtg taacgtctta gtaaagtcta taaaaaaacg
    35701 aaactatcga ttaagctaat agtttcgttt tcaacattta taagtaaact ttttctattg
    35761 gtaatataga ttttcggaag ggtagacagg tgcgcaggta atatcgattc ctagtttttt
    35821 caatgtttgt tcatcatttt tattgaccat cacagtagag tgggcttgtg ctccctcaag
    35881 ttcagctagt ttatcataag caacttgagc gattggattc gttactgcac tgattgataa
    35941 agcgatcaag acttcattga tgcttagtgc atccaaagag gaatgaagtg tctttgattt
    36001 caatccttgt attgtttcaa gaactaatgg tgacaataaa ggaatgtcat ctgcgatatg
    36061 ggctaaatgt tttactgcat ttaagataac tgccgctgac gcatccatca atgaacttgt
    36121 acgcccggta atgatgactt catctggtaa ttcgattgca atcacagcag aaggatcttt
    36181 tgtaggactt tgcgcttgaa tcttatctgc atagtcatgg gcagggacta ccacgtttcg
    36241 gtcttcagga cgtaaaccga cttcttccat aatcagtttg attcgattaa ctgcttcttc
    36301 gtcgatcaat ccttttttga agtcgcattc tgtttggaaa taacgacgga tgatttcttg
    36361 tttagaggct tcttggacaa cggtatcatc cacgatccca aatcctacac gattcacacc
    36421 catgtcagta ggagattgat aaacagattc tttaccagta attttttcaa taatacgttt
    36481 gattacagga aatgtctcaa catcccgatt atagttcaca gctactttat tgtaggcatc
    36541 aaagtggaag gaatcgatca tattgacatc ttttagatcg actgtggctg cttcataagc
    36601 aatgttcagc ggatgtttca acggaacatt ccatactggg aaagtttcga acttggagta
    36661 accagctgca tgtccttttt ggctttcatg atacagttgg ttcaaggaag tagcgagctt
    36721 tccacttcct gggccaggag ctgtcactac aacgattggt ttagttgttt caatatactc
    36781 attttttcca aaaccatctt ctgagacgat tttttctacg ttgactggat agtcttcgat
    36841 ttctgagtgt ttatagacct tgatgtttcg tctttctagt ttattgataa agactttcgt
    36901 ggcaggctgt ccactatagc gagtgataac gacactgttg attaataagc cataaccacg
    36961 taattcatcg attaaccgca agatatccat atcataagta atcccataat caccacggat
    37021 cttattacgt tcaatatctc cagcatagac gcaaatcagt atttcagctt gatctttcaa
    37081 tttttgcagc aatttgattt tcgcatcttc ttcaaatcct ggaagtacac gtttcgcatg
    37141 cttgtcgtca actagcttcc caccaaattc taaatataat ttgtcatagt ggttcactcg
    37201 ttccaaaata tacgctgact gctcttcaat atatttttct gaatcaaaac caattttttt
    37261 cactaggttc cctccttaag tactaaagca ttttttcatt tttttgagga ttcgtcaact
    37321 ggaagagcga aattcctcaa aaaaacaaag aaaatggttt aaaccaactt gtctagatag
    37381 ttattcattg gtatgatagt ttttgataag gaaggtgcta catgaaaaaa agacagaaaa
    37441 agaaaaacgc atataaacac tatatccgtt cgatttttac cggttatgaa agaatgcttg
    37501 aagatcccga attggaacaa ttgacgttta cttatctaaa cgaagaaacg cagctaactc
    37561 gtgatgagca tcagcgcatt catttcacaa caagagatct gcctagtaaa taaaaaataa
    37621 gataaaggtc atcggattcg attctgtcct atcagatgga aagactagtt gatcgaaaaa
    37681 aaggctgtac caaattttgg tgcagcctgt tttatggagc attgcccgct cttttttata
    37741 acaatttttt acagaaaatg gtactattga agtattaata agtgaaaaac agatgagtaa
    37801 gaggaggcaa gtagtatgaa agagccagat acaaatgcaa attatgagcc agacgaacta
    37861 gaggagacga atgcggatca ggatacagga caagaatcgt cttcttttta cgatatcaaa
    37921 gaaaaagcaa gcatgaatct ttcccaatta ttagaagact tacaggcttt tgaaaaagct
    37981 gtccgcgaag aagatatccc tgagatctac cgtttataca atggccgatt gaatgaagaa
    38041 ttaaaaagaa gctcaaatga aaatcacgag atcgatcaat tgctgatgcg taagatccat
    38101 gatcgttttt tacaggaatt tccttttatg gaacaagtag aaaacatctc gccaacgatg
    38161 agctattaca aaatcggcac ttattaccat gatcggccca cggtcggtat tgatgcaagt
    38221 ctgccagaaa tttttgtttt gcctaagatc gatgaagagt gggagaaata ttcccaaccg
    38281 gagtcaacgg actatgacaa aaaaatcaat gagctagacg caaaggtgat cactgctcag
    38341 acagaaattg aacgtttaga tgaacaaata aaagaaatca atcgacaaat gactgatttg
    38401 aacgacaata aaggattctt gaatcgtaaa aaaatcgagg aagaaattca agaattagag
    38461 aagaagaagc aagtactatc taatgaaaaa ttaggctggc ttccttatat tgagacgcct
    38521 gaaacaatcc agcagcaaaa agaagcattg aagcaagaag caagagcaga ccagcttcgt
    38581 gcagccatcg tagaaaaaga acaaaggcaa atcagacgct atttcggtag caaagaggga
    38641 ttcggtcaag caatccatga attcttgatg aattatcttg gcaatgagaa tcctaataat
    38701 caatctaatg aaggaggttc cgagtatgaa tgaacacgat gaagcagaat ggacaaaaaa
    38761 tgaagaacaa acagaagatc atgaagaaac gaacacttca ttggaagaac aaacaagtaa
    38821 tcctctggat gacacagaag aaatccctat gctagaggaa acaaaaatac acgaaaaaga
    38881 aaaagtaact ccgacgaata cgaatactgc agaaaacacc catgcttacg aaataactga
    38941 aaatcccggg tatttagaag gaaaagagca agatcatcaa gatatcgtca gtttgattga
    39001 aacagcggaa gcagaattag cggatttacg tatgagaaaa gatttgatcg aatcagattt
    39061 tccacaatta ttagaatcat atgatcgttt tatattgaat gaagaaccaa tctccacgat
    39121 cggaaagaca agcctgatcg aatacttcac gtatgaatta ctgaaaccgt tgaatgatat
    39181 tggtctggaa cggtcaaatt cagcttatga cacatggaaa acaagacatt tttcgatcaa
    39241 ttatacgttg aatggacaaa acgaattagt cttttccttt gtattaccga ccatcgatca
    39301 acgataccaa gtggaatcga tgagtctcct aaacgtatca ccagagacaa tggaaatcaa
    39361 tgtccaagat gaccgggtgc tttctttgat ccgacactgg tcagtggatc gagtattctc
    39421 tgcagggcag atcacgatat tcaatcacaa attgaatcaa ttattagccc atgcaagaac
    39481 acttggtttt accgttaatc agacattact agacaatacg aagccacttc gtttgaatct
    39541 tcagagcgaa tttgaattga cgaatgaagt attggatgat atttttatcg tggcaatgaa
    39601 aaatccatcc tacgatatgg aaaagatcaa cgaaactact tatcaagtac gattagataa
    39661 aggacagtct ttaacgataa caacaaacga aaaagatcaa acagtcttgt cgatctcatc
    39721 tggagactac ccacgatctg tcattgattt ctttatcaat tacgaattct tggtcccatt
    39781 aatggtgaga aagtcctgat tggataagtt ggattaccag atgtcattca gataattctg
    39841 ttccaaagga cttgcggcag ttatccagaa taattgtcac aagtcttttc tgtatgtact
    39901 aaatggacgt gaataacttg ctgatgaagg agggatttca ttaggaatgt ccacaaataa
    39961 aatagcacag cttagcttat tgtctgctgc ttgtgtagca ggtaggatcg cttttcaatt
    40021 tattccgaat ttccagccaa tgactgctat ttttttattt atagtacttt acttaagctt
    40081 aaaagatgcg ttaattgtca tgagtctttc tattgcgatc agcagttttt attttggtgt
    40141 tggaccatgg gtcatcggcc aatggatcag ctatactgtt gttctgagtt tgttttcaat
    40201 cgtctgcttg agaaaaacag tgcaaaggaa tctatggtta aagggagtct gtttttttct
    40261 ggcgggcatg gtttacggaa taggtatgac cgtttttgat acgctgttat atcgtttgcc
    40321 tcaaccttgg atctattacc ttcaaggggt gtcgtttgat ttgatgcaca gtataggcaa
    40381 tgttgtgttc tttcttgttt ttctgccagt cgtcaaacgt ttttacaatc attcaggagg
    40441 aaaaaatgaa aaaaataatt tatagtttgc ttgcaatgag tgtcagtata ctcattttga
    40501 caagttgcag tacaacctat acacaatcgg cttccaacca aaaggaggaa gtaaaaacat
    40561 cgcaaaaagc aacaatcacg ttacaagaaa atggtcaaaa cttttcggaa aaagaagtgt
    40621 tcttcaaaaa aggcgatgat ttactgagta ttttgaaacg aaattttgaa gtcaaagaag
    40681 ataacggctt catcacttcg cttgaagggc actcacagga tgagaaaaat caactctatt
    40741 ggatgtttac tgtggacggg aaaagtgcta ctactggtgc caaagagatc aaattgaatg
    40801 atggacaatc ggtcattttc aatttgtcta aactataagt ttcataaaaa actgttaaag
    40861 ggcgaatatt gccataattc agtgagttca gttgatgaaa gaaggaaaat aacatggaaa
    40921 attttaaaaa aagcggaatg ttttccgtaa ttgctgctct aggggctaat attctagttg
    40981 ctatcagcaa atttgtcggc tatgcaatca gtggcagtgc cgccatgcta aacgaaagca
    41041 tccacagtat cgttgattgc agcaatcaga ttttgctgtt attcggtgat aaacaagcca
    41101 ataaaggaca gagcgagttg catcaatttg gtgaaggacg agcaaaatac tttttcagta
    41161 caatcgtcgc aatgatgctt ttcttcggcg gtggggcact tggggtgatg gaagcttttg
    41221 aaaagttgac gcatccatcc catgaagtgg gtaatccgat cattgttata gcaatactgt
    41281 tatttggttt ggtaatcgaa ggaagttcgt tacgcgtggc aatgaaagaa atcaaagaat
    41341 tgaatactga aaaactttcc acgatgcgct ttttgcgcga aagtcgacac agtgaaatac
    41401 tgattatttt tacggaagac ttgtgtgctg taatcggttt ggtcttggct ttagcaggaa
    41461 caatattgac ttatgcaacc gggattgctg catttgatgc gatcagcgga ttgctgatcg
    41521 gtttcttgct gatgggagcc gccattttcc tagcaaaaga attttatagt ctgatcatcg
    41581 gtgaaagcgt gacagcaagc gatttatcaa aaatcaagtt agcttttgaa cgtcctgaga
    41641 tcagtcggct gatcgatata aaaacagttc atttaagtcc aacggagatt ttagttgcag
    41701 caaaagcaga tatcgttggt aacaaagaga atgaagctta ctcggtcatc aatgatattg
    41761 aaaaaaatat gcgtcagtct cttcctgaca agaagatgta tatatacatt gaaacagata
    41821 aatatgaccc aaattattct cgtaaataaa acgtttattc atgaattgaa gcaaaaatga
    41881 gaaaaatata atcttgtttt taaataaaac ggctacaatt tagcgaattt atgttacaat
    41941 gagtccgctg tttatttgtt gtaattaaag gagatatttt aatgaacaaa aagaaaagtg
    42001 cgccatggct tcaagctttg catgcagtga tgccactttg tataagctat ataccagtag
    42061 gattagcttg cggagtattg ttgcaaaaag ttggatttga tcctctctta tcaggactgt
    42121 tatcgattct ggtcttttcc ggtggcgcgc agtttttagt tgcatctatg cttacgacgc
    42181 aagcatcatt tgcaacgaca ttattgatgg ttttcttttt agaattacgc tatacattat
    42241 taggagcaag tcttgccggt tttatgaaaa aagaaaagcg gacgtttttg gctgtctttt
    42301 ctcaatcact gaatgatgaa aactatgcgg tgaactactt aaagttttca actgaccctt
    42361 cctggaataa aagaaaggct ttatatgtca attggttctc aatgactgca tgggccggta
    42421 gcaatatgat cggtaatttt ttcggctcgg tgattcgagt agatgctgac cttgtccatt
    42481 ttgctttgac ggccatgttc attttcatgt ttatcatgca gatgaaaagc gcactactga
    42541 tctttacagg tttattctca ggcgtattag gggtcgtatt catggtatta ttccaaaata
    42601 ctttcggatt gattgcagca acagtcattg cttcatttgc cggatttatg ctggagaagg
    42661 cattcaaaaa agaaaaagct aagaaactaa gagaccgcgg taaagatgtc aatgagccgc
    42721 tcttcaactt cccggaacag gaggccaatc accatgacta aactatattt gctactttta
    42781 gttgcttgtc tgttcatcgt ggcatatatc ccgcgcttgt tcccgatgct ttattttact
    42841 catcgaaaag tgccatcctg gttcagtgac tggatgaaat atgtaccggt ggcattattt
    42901 gcggcgttag cttttaaaga tgttttcatt actcatgaac atctggatat tgcttggaat
    42961 atcaaaatag ctgcaatgat tctagtagca ggcgtggctt acaaaacacg ttcgatggcg
    43021 ttgtcagttt tgacaggact tgcgtctgtc tttttgctgt ctatgcttta aactgtttta
    43081 ttttattaaa aacagagatt gcgacataag ttgttcaaac tgatctatcc gaactgcatt
    43141 tgcaacagtc aattctggat gcaagtttat tcgtagggat aaaaaagatt agtgttggct
    43201 tatgatgcaa gctctttttt attttaggaa ttagagaggg atttgtcctt ctatgatttt
    43261 ttgctataat agggaagatg tttattttat gaaagaatag ttgagggaat ttaaatgaat
    43321 aaaaaattga tgcaaggaac attttggcta acctttgcta atcttctatg taaagtttta
    43381 ggggtagtct atttgattcc ctggctaagt atgatgggga acaatcaaga tggtatgctt
    43441 gctacgacat tgtataatgt tggctatctt ccttatggat tgttcctgat gcttggtaca
    43501 gtcggatttc caaatgcgat tgcaaaaaaa gtagctgtag ccacaaaaga aggagataac
    43561 gctgcttgtc ggctgatttt tagaagtacg atcaatatca tgtttgtcat cggtatcgcc
    43621 tctgctgcac tgatgtattt atttgctcct ttactagccg gaatcagtcc gattgctaac
    43681 gttaataatg ggatactagc tatcagaagt ttatgcccat ctttgattgc tatcccaatt
    43741 cttagtgcaa tgagaggata tttccaagga aaaaatgatc tgcggcctta tggaacttct
    43801 ttgattatcg aacaggctgt gcgagtgatc gttatcttgg ctggtactta ttacttgcgc
    43861 gtattgactg acggaaccat attagaagct gtattgatca gtacagtcgc ttctttcttc
    43921 ggtgggttag ctgcgattat ccatatgtat atcgtcggac gcagaaaaga cttcttcgaa
    43981 cttaaagatt ttttgatttc cagccgttat ttcgaaagag aaaaccgatc tgcctctgtc
    44041 tctatcattc gggaaacact accgtttatt tttgtcggct cagtcattac tatcgttcaa
    44101 atgatcgacc aagtaacgat gaaaccactg cttcactttt tccgtccaga gatcgcagat
    44161 cagcaactgg aattcttatt cagccgtgct tctgtcaatc caaataaatt gacattgatc
    44221 ctgatctcca tggtaggaac agtagctata agcagcttgc caatattaag cacgatgaag
    44281 aaaaaagatc ggatacaaat cgaaaaaaca gtaggagata gtttttcgat tgcgttattg
    44341 attcttcttc cttcattgac tggaatgtct ttattagctg gaccactata cacattgttt
    44401 tttggctatg atcctgaaag tgtcggatat ttccaaatgg ctttgctagc ttctttgttt
    44461 ttctctttat tcacgatcct atctacgatg cttcaatctt tgaatcacca tcgttttgcg
    44521 atccgattaa ctgtagaagc aattatcctg aaagtcatct tccaagtaat cggattagga
    44581 ttagttggcg gatacgggat gagcttatca aatggcttag cattcggtat tgtctttgtt
    44641 cgcggctatc actttatgtg caaagaatac cgaatagcac ctttagcaaa gatcagcaat
    44701 tttttcttaa aaactttccg ctccaccctg attatgcttg cagtatgttt tgccgttttc
    44761 tttttgctga atcagcgatt gtcaatggaa tcaaaaagct atgcaatgat ttattgtgtg
    44821 gctgtaggaa gtattggcgg cttagtattt ttattttctc aattcggaaa aaatgggttt
    44881 cgaatgttga aaaatgttaa acatcatagc agaagcaaag aataggttaa ctaatcgttt
    44941 tttgctgtca cttgaaaaac ggcaactcgt atgattacgc ttgtcgtttt tctattttcc
    45001 aattttagtt tcatatctaa tcttacttat gatatagtta taaggtctgg aaaaattctt
    45061 tgaaacgagg aaattaaaca catgaaactt gcagtagtta cagatagttc ggcatatctg
    45121 ccggaacgta tcaagaacca tcccgactta ttcgttattc caatcccttt gattatggat
    45181 gggaaaattt ataacgaagg aatcgatatc gaagcggatg aatattatgg acttttaaaa
    45241 aacagtaaag attttcctac tacttcacaa cctgtcgtag gggaagtttt gggtttatat
    45301 gaggaattga aagagaaagg gtatgacacg attttaagta tccatctttc ttcagggatt
    45361 tctggctttg tcaacacatt atttgcgatg aaagatgaca tcagcggagt aacggtcatt
    45421 ccttatgact ctaagatcac tagtatgccg atgggacaca tggtagaagc tgccttagac
    45481 ttaaatgatg aaggtcagtc ccttgatgaa atacttgcac atatcgatcg aatccgagat
    45541 aatacctacg cttatctgat tgtagatgat ttgaacaatt tagtacgagg aggacgtttg
    45601 acaaatggtg cagcgctgat tggcagctta ttaaaaatca aaccgatcct gacattttct
    45661 gaagggaaaa ttgttttatt cgaaaaaatc cgttcaagta aaaaagcatt tgctcgagct
    45721 gaagaaatta ttggtagaag aaataacgaa atacaaccgc cagtcaaact ttacgtgata
    45781 catgcaaata atttagaagt agcgattgcg gagaaagaga aattgcagaa aaaatatcca
    45841 aatgcagaaa tcgaaattgg tcatttcgga ccagtcattg gtacacattt aggtgaaaaa
    45901 gccatcggca ttgccatttc agcgcaataa ttccttttat tcaaaaaaca taccaaaaat
    45961 attccaacat catcttggac tttctacatg ttactaatga gcgtgtaaaa gaacgagatg
    46021 gtgtttttat ttctatcaat gaagctcttt ttctttgcaa tcctcacttc aatcctctat
    46081 aatgtcgcat atagtttagt aaatgagtaa actactaatt tacttattta ctaaaaataa
    46141 aaggatgagg agttaaaata atggaaatac caacaaattt aaaacataaa ccagtaatcg
    46201 ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt aaatacggat gcaaaagggc
    46261 tttcactggg ccttgctcag tggaatgatc gtggaaaagt cgatatctca ggcaaggtat
    46321 ggcgacatac tggagaaaaa tggtcacgac agtcggaaga attaccaatc acacgtattt
    46381 tagatttagc gatcttagct gcacaaggaa gtttatactt ccaagatgct tatcgttatg
    46441 aaaaatttta tgatccagaa aatccagtcg tagacatcat tggattacaa gggaaccgaa
    46501 tgacggtaga agtagatacc aaaaatccaa agatcgatga agacatcatt ttgtattacg
    46561 atacactaca aaaagacgga gaactgattg gggaacgatt ccgtatttta aaacgtcttt
    46621 tagatgagct aggatattaa tagacagaga tgatcaaaag gggaagagaa gcatggagaa
    46681 tttctcacta acagaaaacc gaaccttttc acggctagaa aagcaaatga tctggaagaa
    46741 accgaaaagc catcggacaa gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa
    46801 ttggcaagat cctgaaatga aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg
    46861 gaagacacaa ttagccaaag cactatctgc tgatctgcaa cttccctata caaaagtgac
    46921 ctgttttgca gatatggaca aatcagatgt tttcggagcc ttattacctg tagtagattc
    46981 taataaggaa caagaccaag aattactaga agctatttac caaacagata cacttgaagc
    47041 tgtactgaca ctgatccaga accattacgg cattgatcct ttttctgcta aagaaaaatt
    47101 agcagagcta gttcaaagga tcgaagctga ttatccagat gcaatccatt acagctatta
    47161 cccttctgaa atcgttcgag cgattgaaaa aggatattta ttagagattc aagagccgac
    47221 tgtcatccga gatgcctctg ttcttgtggc acttaactca gcgctcgaac caaatgggat
    47281 gttgaattta ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg tgatcactac
    47341 aaatcgaaat taccaaggaa atcgcccatt aaatgaatct ttaagagatc ggatgcaaca
    47401 tgcggaaaaa atggatcttc ctgccttaga agtcatggca gaacgtgcga tggcgaaaac
    47461 tatgatccaa aaacaagaac ttctcttaaa gatggcagag attatccgtt tgttggatga
    47521 aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt tttattgggc
    47581 aaatacctat aagcaaggac aagatttact ccagtcgatt tatccaaaag ttttgtataa
    47641 attatccact gattcagatg aattggcaat tcttgaacaa gctttgtctg acagtggatt
    47701 gctactggaa cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc aatctgagca
    47761 tacaaaagga agaaccatta gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc
    47821 tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac caaatgaggg
    47881 agaagagaaa caagaagaaa caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga
    47941 acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa caaacaggag atgattctca
    48001 agaaatgtct gcttctgaca tggaagcaca tgataaggaa atcaagaagc aactgaataa
    48061 agaagcgcga caaatcatga aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg
    48121 accagctttc caaggaaaaa cgccggaagc acgtgagcta cacatgcaag taatgccaat
    48181 agtagaatca cttagccggc aaattttaga gttactggat aatgaacaaa cagagacgta
    48241 ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc aaagtagcat atggtgattt
    48301 gagaaatttt gacaaaaaga acccgccaca tgagcaacct tctttagcgg tagctgtacg
    48361 gatcgatgaa tctgggtcaa tgatacggga tgatcggatc gaagcggcaa aaaaagcagc
    48421 gattgccatt gccgaatttt ctaagaaagt cgatatccca ttgttgattt acggagatac
    48481 agcagaccgt tcgtcaagag aaaagacttc tttgttttct tataaagaat ttgaagacgg
    48541 atttcaatgg ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca atcgtgatgg
    48601 tgctgcactt cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt
    48661 gcttaatatt agtgacggtc agccaaaagc gttgccggat tatacaggca aaaaagcaaa
    48721 agaagatatc cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc
    48781 agctattggc caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga
    48841 tattactgac ttgaatgaat ttcccaagca aatgattcaa ctgattgccc gctatctatg
    48901 ataaaataaa gctcaacgaa cgcacttagc agattcgttg agcttttcgt ttctagttac
    48961 cggtaacaag gatacttcta aaatagaatc ggttataatc aagagggacc gttaaaggag
    49021 tgaaacgata tgatacaaga acaaaaatgg tggaaagaag cagtaggcta tcaaatctat
    49081 cctgctagtt tcaaagatag caatggcgat ggtcaaggag acatcaatgg gatccgtgaa
    49141 aaacttaatt atttgaaaga tcttggtatc ggctttatct ggatcaatcc gatctatcaa
    49201 tctccatttg tcgataatgg ttatgacgta agtgactatc aagatataga agagagattc
    49261 ggaacgatgg aagattttga tttattgcta aaagaagcac atgaattagg gatcaaagtg
    49321 attatggatc ttgtcatcaa ccatagttcg gatcagcatc aatggtttga agaatcgaaa
    49381 aaatctaaag aaagcccgta tcgcgattat tacatttggg tcgacggggt agatggcaaa
    49441 gaaccgaata attggacatc gattttcggc ggttctgctt gggagtattc tcatgaaact
    49501 ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag
    49561 aaattgaaag aagaactatt caatatgatc cgatggtggt tggataaagg gatagatgga
    49621 tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa agccacagag
    49681 aaccctttct caccctttca aaatgtctca ggaatcgaag aacatttgac cgatttgaaa
    49741 catgtttttg aagagtacga tatcatgact gtcggagaag caagtggtgt cactgcagaa
    49801 gaaggaccac aatgggtagg gaaagacggt tatttcgata tgatattcga atttgatcat
    49861 atccatatct ggcagcaaga aaaagaaggg caattagatg tccttaaatt gaaacttgca
    49921 ttaagcgctt ggcaaacttc attggatggt atcggatgga acgctttata tatggaaaac
    49981 catgatgttc ctcgtgccgt ttcagtgttt ggagatactc gtcccgattt ttgggctatg
    50041 tctgcaaaag caatcgctat gatgtatttc tttttgcaag gtacgccatt tatttatcaa
    50101 ggacaggaaa tcggaatgac gaatatgccc tttgaatcga tcgatcaagt tgatgcagta
    50161 gattcgaaaa gactttataa aaggctgctt gcggaaggta agacaagaga agaagcattg
    50221 gatatcattc gtgaaacaac gagagacaac agcaggacac ccatgcaatg gacttcggaa
    50281 caatatgctg gtttttctac acatgaacct tggctgatca ctaatccaaa taccaaaacg
    50341 atcaatgtag aacaacaaga atatgaacca gagtcggtcc tgcaattcta taagaacatg
    50401 atccgcatta gacaaaccaa taaaggtctg atatacggtt cttataaaga atatcttcat
    50461 gaacatccac aactttatgt ctatgaaaga tatttagaag atgaacaata tcttattatg
    50521 gtaaacttaa cagaatcact tgctgactat gaacttccaa aagaagcaga tcagtcctgg
    50581 acattactct tgtcaaattc ctctagcgga gaatttgaag ctaaaggcat actagcacct
    50641 tatgaagctc ggttatataa aacaaacaag taaaacttcc tcgttaaagt tcattagttg
    50701 gactttgcga ggaagttttt cagattgctc tgtcaattac tcatttcaat aattcgctct
    50761 aggaccaccg atatatttag gacgagagcg aagggctgtc acgtgagctt gtccaagggt
    50821 tttcaaacga ggtctgacag gtacttggac atgtttgata tgcataccga ttgatgtgtc
    50881 accgatatcg ataccggctt gtccaactac gtgttccact tcgacaggat cattgaactg
    50941 gtcaaaagca gcaacagaac aagcacctcc agcatgtagt gcaggaacaa cagagacaat
    51001 ttcgaaattt tttgcttctg ccactgttcg ttctactact aatgctcggt tcaaatgttc
    51061 acaaccttgg acagctagcg caatttcttt aggatccaac aactctttta acgtacggat
    51121 gatccattga ccaacttccg cactggaatt ttttccaata tgtcctccaa cgacttcgct
    51181 tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc
    51241 attaactatt tcagtcaatt ggtcttttaa ttctttttct gacaacttca ttttctaaaa
    51301 ccttcttttt caattaatat tgcttccgtg cttgaatacg ttgaagactt gcagttatag
    51361 gaaaggcaag tcccattcca atcagatttt ggaagatgtt tcctggtata gaagccactc
    51421 cagccggcca atcatataaa atccaaccgg cgataccata gccgatgatc ataactaagc
    51481 ttcctaataa aagccctaat ccccacatac gagtagaaga tttgctgaaa gaaccagcaa
    51541 catatccttg taacccgtgg atcactaatg aaaaaactgc ccactgcgga tatcctgaaa
    51601 tcaaatcgat caatagacca ctggaagcac caacaactaa tccacccgtt ggaccataaa
    51661 gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac aaatccattc gtagccggta
    51721 cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg
    51781 ttaattgctt agttttcatt tctcattcct cctttttgaa taccgccaga ccagacactt
    51841 ccggtatgat cgttcatcag cacgccatta gaaatacaat gggcaacaaa ttttttactt
    51901 tctgctactg ctttggtaaa atctgcccct aacacaagat tcgctgttat tgcagaagaa
    51961 aaggcacaac cggccccgtt tactgttgta gtgtcgattt ttttatgaga aaatacctca
    52021 taatgatcaa ttgaatagag aagatccaca gcttcatctc cagccatcct tgtgccgcct
    52081 ttgatcacaa tgttttttgt tcctgaagca gtgagttctt tagcgagatg gatcatttct
    52141 gttaacgaag agggaagagc ttgaccagaa agtaaagcag cttctgccaa attaggcgta
    52201 atcatcgtaa caagtggaaa aagttcttcg atgagctgcg aaatatacaa ttgattgatc
    52261 acagcttcag tttctttgaa tgccaaaaca gggtctaaaa tgattgggat attttgtttt
    52321 gtttttatga agtctcgaac aaccatgatt gcttcacttg aattcaataa cccaattttg
    52381 ataccatcta atgaagtcat tttatcaatc gaaactaact gtcgtttaag caattccaaa
    52441 gatatagatt ccagcataaa tcgctcatta atatctagtg cccctataga agtcaaggcg
    52501 cttagtccga atagaccaaa attctcaaaa gtttttaaat ctgtttggat tcctccacca
    52561 gcaaatggat cagaaccgcc aattgtcaag attcttttca taacattttc ctccatgaat
    52621 aagagtataa cgaaattttc aaaaaagaaa aacagccaat tggcatattt ttatctcatc
    52681 caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga tctttctttc cttaaatagt
    52741 taggactttt gtgattttaa ttctttctcg atttgaagcc gttgcacttt cattgtagct
    52801 gttcgcggga ttgcatcaaa atcacggatg atagggacgt tcaagcgagg aagttcatgg
    52861 acttgctccc accatcgatt ccaatccatt tctttatcag gagctaaagc aataatcggt
    52921 tgtggagaat gatttttatc ccttacaatc actacttctg ccaaaaaatc taacgaatct
    52981 aataaaaagt cttcaatagc taatgtacta ttgatcgttt cgatcaaatc cacttgccga
    53041 tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt aatctccgct atcccaccaa
    53101 tcgccataaa cattttcctg gaaccgtgca tcttccttgt aataagtcaa agcccgtcct
    53161 ttagacagta attgtatatg accatctgtg ttttcaggta ggacattccc aactgaatct
    53221 gtaatccgag cgcttgtcat gtcttccagt ccaacgccca tatctcttgc atcacttgtt
    53281 ttcaaactct ctagtgtatg agccttaaga atcattggtc cgcattcact ttggccatat
    53341 acttgaagaa ataccgcatc ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg
    53401 ttgtttatag catcaaaagt ggagtgatag tatcggatac ccgaaaaagc ttctggatgc
    53461 tccttagcag taaaacgcca ctgaacaaag ttattcggat gggtctctag tgcaattggc
    53521 cggtgagctt ccaacatatg aacaactttt gaactttgtg cgtttgctaa aggcatcata
    53581 ggaaatccca tggacatcaa agaagagata ccaatattga atctggaatg cactggagag
    53641 atgtgaaaag cgattaattt cttttccgaa atttttgtga aaactgtttt ttgccatttc
    53701 gtccgccatc ccatagaatg agcagaatga caaataagtt tagggatacc agttgtacca
    53761 gaagtatggg tcatataaga aatttcattt ttgtctaaaa atgattgaga aacagacaaa
    53821 gcaggttgtt ccattaaata aagaatagg
//
LOCUS       DACDRN010000010        52063 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDRN010000010 DACDRN010000000
VERSION     DACDRN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.010"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..97
                     /locus_tag="IWJ80_000831"
     CDS             <1..97
                     /locus_tag="IWJ80_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9460589.1"
                     /translation="ILGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(127..1542)
                     /locus_tag="IWJ80_000832"
     CDS             complement(127..1542)
                     /locus_tag="IWJ80_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9460590.1"
                     /translation="MSNKEEAKSIINFVGGKENIAALTHCVTRLRFALRDDTKVDKAG
                     LENLEDVKGTFNANGQFQVVIGPKVADIYEEVISQSGLSGANKEDVKKVAAELNLNWF
                     QRAVKILGDVFVPILPAIVTAGLLMGLNNLLTNPGIFFEQSLIEVYPNIADVADMINL
                     IANTSFQFLPALITWSTVKYFKGNPLLGIVLGLVFVHPDLMNAWNYGQAVTTGDIPHW
                     TIFGLSISKIGLQAQVLPALVSGYVLAKTELFLKKRVPETIQLLVVAPVAILFTSVIT
                     FIIIAPITIAIGNTIVAGIVWLFQKVPFIGGFAYGIGLAPLVVTGMHHAFLPVHIQLM
                     ANGGDYIWPIGVMSSLAQGSAAIAMNFLIQNKKLKGVGLAAGLSAYMGITEPAMFGVN
                     LKYKFPFVVSILTSGIIGGFMASQHVIATSVGISGIPGFLSIPFEFWPIFFICMLASC
                     VVSFGATYLYGKNKVLLQSFK"
     gene            complement(1535..1903)
                     /locus_tag="IWJ80_000833"
     CDS             complement(1535..1903)
                     /locus_tag="IWJ80_000833"
                     /inference="COORDINATES: protein motif:HMM:NF025668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460591.1"
                     /translation="MSYTTFSMSPISIKEKGKNCLKVTTTVSNKGSRDGYEVVQLYIG
                     AQYGRYIRPTKDLVAYKKVFVKSGDSVTVSFELTNKDFKYYDEEFNEIIDDGAYWIMV
                     GNDSSQALTNKEEIEVRINE"
     gene            complement(2990..6139)
                     /locus_tag="IWJ80_000834"
     CDS             complement(2990..6139)
                     /locus_tag="IWJ80_000834"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAP9460592.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(6152..7369)
                     /locus_tag="IWJ80_000835"
     CDS             complement(6152..7369)
                     /locus_tag="IWJ80_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9460593.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            complement(7359..8954)
                     /locus_tag="IWJ80_000836"
     CDS             complement(7359..8954)
                     /locus_tag="IWJ80_000836"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9460594.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            complement(9464..10246)
                     /locus_tag="IWJ80_000837"
     CDS             complement(9464..10246)
                     /locus_tag="IWJ80_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460595.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(10311..11147)
                     /locus_tag="IWJ80_000838"
     CDS             complement(10311..11147)
                     /locus_tag="IWJ80_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9460596.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(11165..12574)
                     /locus_tag="IWJ80_000839"
     CDS             complement(11165..12574)
                     /locus_tag="IWJ80_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9460597.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(12588..14411)
                     /locus_tag="IWJ80_000840"
     CDS             complement(12588..14411)
                     /locus_tag="IWJ80_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9460598.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(15254..15580)
                     /locus_tag="IWJ80_000841"
     CDS             complement(15254..15580)
                     /locus_tag="IWJ80_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460599.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(15626..15988)
                     /locus_tag="IWJ80_000842"
     CDS             complement(15626..15988)
                     /locus_tag="IWJ80_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP9460600.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(16025..16240)
                     /locus_tag="IWJ80_000843"
     CDS             complement(16025..16240)
                     /locus_tag="IWJ80_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9460601.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            16367..16702
                     /locus_tag="IWJ80_000844"
     CDS             16367..16702
                     /locus_tag="IWJ80_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9460602.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(16778..18775)
                     /locus_tag="IWJ80_000845"
     CDS             complement(16778..18775)
                     /locus_tag="IWJ80_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460603.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(18873..19148)
                     /locus_tag="IWJ80_000846"
     CDS             complement(18873..19148)
                     /locus_tag="IWJ80_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9460604.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            19380..20114
                     /locus_tag="IWJ80_000847"
     CDS             19380..20114
                     /locus_tag="IWJ80_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9460605.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEKFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            20153..21295
                     /locus_tag="IWJ80_000848"
     CDS             20153..21295
                     /locus_tag="IWJ80_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9460606.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(21390..22781)
                     /gene="sufB"
                     /locus_tag="IWJ80_000849"
     CDS             complement(21390..22781)
                     /gene="sufB"
                     /locus_tag="IWJ80_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP9460607.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKAVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(22786..23256)
                     /locus_tag="IWJ80_000850"
     CDS             complement(22786..23256)
                     /locus_tag="IWJ80_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP9460608.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(23243..24478)
                     /locus_tag="IWJ80_000851"
     CDS             complement(23243..24478)
                     /locus_tag="IWJ80_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9460609.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(24475..25761)
                     /gene="sufD"
                     /locus_tag="IWJ80_000852"
     CDS             complement(24475..25761)
                     /gene="sufD"
                     /locus_tag="IWJ80_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP9460610.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(25774..26547)
                     /gene="sufC"
                     /locus_tag="IWJ80_000853"
     CDS             complement(25774..26547)
                     /gene="sufC"
                     /locus_tag="IWJ80_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP9460611.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(26744..27592)
                     /locus_tag="IWJ80_000854"
     CDS             complement(26744..27592)
                     /locus_tag="IWJ80_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9460612.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(27616..28302)
                     /locus_tag="IWJ80_000855"
     CDS             complement(27616..28302)
                     /locus_tag="IWJ80_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9460613.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(28295..29329)
                     /locus_tag="IWJ80_000856"
     CDS             complement(28295..29329)
                     /locus_tag="IWJ80_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9460614.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(29724..30062)
                     /locus_tag="IWJ80_000857"
     CDS             complement(29724..30062)
                     /locus_tag="IWJ80_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP9460615.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(30064..30420)
                     /locus_tag="IWJ80_000858"
     CDS             complement(30064..30420)
                     /locus_tag="IWJ80_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP9460616.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(30426..31613)
                     /locus_tag="IWJ80_000859"
     CDS             complement(30426..31613)
                     /locus_tag="IWJ80_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9460617.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(31804..32649)
                     /locus_tag="IWJ80_000860"
     CDS             complement(31804..32649)
                     /locus_tag="IWJ80_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9460618.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            32883..33848
                     /locus_tag="IWJ80_000861"
     CDS             32883..33848
                     /locus_tag="IWJ80_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP9460619.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            34225..35268
                     /locus_tag="IWJ80_000862"
     CDS             34225..35268
                     /locus_tag="IWJ80_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP9460620.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(35330..35794)
                     /locus_tag="IWJ80_000863"
     CDS             complement(35330..35794)
                     /locus_tag="IWJ80_000863"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP9460621.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            36040..36987
                     /locus_tag="IWJ80_000864"
     CDS             36040..36987
                     /locus_tag="IWJ80_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9460622.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(37198..39270)
                     /locus_tag="IWJ80_000865"
     CDS             complement(37198..39270)
                     /locus_tag="IWJ80_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9460623.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(39203..39580)
                     /locus_tag="IWJ80_000866"
     CDS             complement(39203..39580)
                     /locus_tag="IWJ80_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP9460624.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(39709..40269)
                     /locus_tag="IWJ80_000867"
     CDS             complement(39709..40269)
                     /locus_tag="IWJ80_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9460625.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            40419..41711
                     /locus_tag="IWJ80_000868"
     CDS             40419..41711
                     /locus_tag="IWJ80_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9460626.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(41771..42889)
                     /locus_tag="IWJ80_000869"
     CDS             complement(41771..42889)
                     /locus_tag="IWJ80_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP9460627.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(42903..44324)
                     /gene="arcD"
                     /locus_tag="IWJ80_000870"
     CDS             complement(42903..44324)
                     /gene="arcD"
                     /locus_tag="IWJ80_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP9460628.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(44570..45691)
                     /gene="mnmA"
                     /locus_tag="IWJ80_000871"
     CDS             complement(44570..45691)
                     /gene="mnmA"
                     /locus_tag="IWJ80_000871"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP9460629.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLIYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            46095..46694
                     /locus_tag="IWJ80_000872"
     CDS             46095..46694
                     /locus_tag="IWJ80_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP9460630.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(46832..48118)
                     /gene="efmA"
                     /locus_tag="IWJ80_000873"
                     /pseudo
     CDS             complement(46832..48118)
                     /gene="efmA"
                     /locus_tag="IWJ80_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
     gene            complement(48389..48733)
                     /locus_tag="IWJ80_000874"
     CDS             complement(48389..48733)
                     /locus_tag="IWJ80_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP9460631.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(48870..50006)
                     /locus_tag="IWJ80_000875"
     CDS             complement(48870..50006)
                     /locus_tag="IWJ80_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9460632.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(50195..51169)
                     /locus_tag="IWJ80_000876"
     CDS             complement(50195..51169)
                     /locus_tag="IWJ80_000876"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9460633.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(51249..51812)
                     /locus_tag="IWJ80_000877"
     CDS             complement(51249..51812)
                     /locus_tag="IWJ80_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460634.1"
                     /translation="MEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVL
                     LAYYQDYRKGWMLPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKK
                     NGNHERARATLLGSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGER
                     YYKVHDNWGDYHKVIPASWSNGTVSLP"
BASE COUNT        15001 a        10940 c         8981 g        17141 t
ORIGIN      
        1 cattctagga ttatataaga aaacccgaaa agaaggcact ctcttcgggt tttcggtctg
       61 tactgaaatc aaggtattat tgggaatccc agcttaaatc atagataccg taagggattt
      121 tattctttat ttaaaacttt gcaacagaac cttatttttc ccgtaaaggt aagttgctcc
      181 aaatgagacg acacaacttg ctagcataca aataaagaat attggccaga attcaaacgg
      241 aatagacaag aaaccaggaa ttccactgat accaacagaa gttgcaatga cgtgttgtga
      301 cgccataaat cctccaatta ttccagaggt cagaatggaa accacgaatg ggaatttata
      361 ctttagatta accccgaaca ttgctggctc agtgataccc atataagcag acaaaccggc
      421 agccagtcca acacctttca actttttatt ttggattaag aaattcattg caatagcagc
      481 agaaccttgt gccaaacttg acatgactcc aataggccaa atataatctc caccgttcgc
      541 catcaattga atatgaactg gtaaaaacgc atgatgcatc cctgtcacga ctaatggcgc
      601 aagaccaata ccataagcaa atccgccaat aaatggaact ttttggaata accatacaat
      661 acctgcgaca atcgtattac caatagcaat tgtaatcggc gctataataa taaatgttat
      721 aacactagta aaaagaattg caactggtgc aacaaccaac agttggattg tttcaggcac
      781 acgctttttc aagaatagct ctgttttagc taacacatag ccggaaacta gagctggcaa
      841 tacttgtgct tgtagtccta tcttagagat ggacaatcca aagattgtcc aatgtggaat
      901 atctcccgtg gttactgctt gcccgtaatt ccaggcattc atcaaatctg ggtgtacaaa
      961 gactagacct aacacaatcc caagaagagg atttccttta aagtatttta cagttgacca
     1021 tgttattaac gcaggcagaa attgaaagga agtgtttgcg ataagattta tcatatcagc
     1081 gacatcagca atattaggat atacttcaat gagtgactgc tcaaaaaaga ttcctgggtt
     1141 tgttaataaa ttattcagtc ccatcagtaa accagctgta acgatcgctg gtaaaatcgg
     1201 cacaaataca tcacccaata ttttaactgc acgttgaaac caattcaaat tcaactcagc
     1261 cgctactttt ttaacgtctt ctttgtttgc cccactcagc ccagattggc ttataacctc
     1321 ttcatagata tctgctactt taggaccgat aacaacttga aactgtccat tagcattgaa
     1381 ggtacccttc acatcttcca ggttttccaa tcctgcctta tcaactttag tgtcatctcg
     1441 caaggcaaac ctcagacgtg tcacacagtg agtcagtgct gcaatattct cttttccacc
     1501 aacaaagtta atgattgatt tagcttcttc tttattactc attaattctt acctctattt
     1561 cttctttatt agttaatgct tgagatgaat cattgcctac catgatccaa taagcaccat
     1621 catctataat ttcgttgaac tcttcatcat aatacttaaa atctttgttt gtaagttcaa
     1681 aagaaacagt cacactatcc cctgacttaa caaaaacctt cttgtaggct accaagtctt
     1741 ttgttggtct tatataacga ccatattgag ctccaatata tagttgaaca acctcataac
     1801 catctctact tcctttattt gaaacggtag tagtcacctt caaacagttc ttacccttct
     1861 ctttaatgct aattggtgac atagaaaaag tggtataact caacccatac ccaaagggaa
     1921 attgtggttc attggataca tctaagtatt tggtcgtatt aaacttttca cttgaatacg
     1981 gtctaccagt attgaaataa ttataataaa tagggatttg tcctaaatgt ctcggaattg
     2041 ttattgtaag ctttccggct gggtttattt tgccacaaag gacgtccaaa atagcattgc
     2101 cagcctctgc tcctagatgc caagcctcaa ctaatccctt acatatagat ttttaaatta
     2161 cacactagtt taaactaaaa caaacaagga aaaacttacc atttaatcaa aaaattagct
     2221 catatgaata tattagaatg taaattagac tggtcaaaat ctggtcaaaa aagaggacga
     2281 atacaggtag tcctcttttt tgttattttg atattctaaa tcatttttta ttggattatt
     2341 cccttgttcg atatagaccc aaattgatct atcaataatt atcaaactga gtcatataga
     2401 gacaaaaatt tcaaaaatga cctgttaaat ggtcatcgaa tatcatttaa aatcaatacg
     2461 acggagtcag acacggagtc aaaacaagcc cgctgagagt aaaaaatata cagtcccata
     2521 ggtaaagtgc cacaggactg tatatttttt tatttgagat tacctgaaat gattccattt
     2581 aatttttatc tcattttttc ctactagtta gtatcaaata ctctcctatt ttctcaataa
     2641 actattttgg gttacatggt atctgaaaca gctaaaacct tgtcattata gcaaaaaatc
     2701 actttatctc cgtttcattt cagatacatt tcacttaagc ggattccaaa aaaacaaaag
     2761 aaaaaggaac ccctagttag cctatgcttt ttattggttc ctctcaaaac acggtcatat
     2821 aacaacgggg tggggtaaat gaatgaaaaa ggtggaggaa acattattca atagctgtct
     2881 taaatacttc gttataaaat gtgaacaaat aaaagacatc tatccctatt ttctacactt
     2941 ccacttgact accatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
     3001 cattctcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
     3061 gtgggtattt aaatggcttc gcttctggat gtttggcttt atatccttcg taatccttac
     3121 tgttgataat tgctttcata ttaggaattg gatcttcacc tggtagatat tgcagattag
     3181 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaagacatga atcaaatcat
     3241 cactcgcttg tgtaaaatag cgacgtttaa tgacaaagat atcttcatta ctatctaaag
     3301 tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct tctgttttat
     3361 tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc gcagcataag
     3421 ttcctagtaa ttgatcaata taagttgaat caatatcata caacttagtg gtattttcac
     3481 tataaaattc gatcgttgaa aaatcttctc cttctttttc tggttcttcc tctattaatg
     3541 aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt tgattgttca
     3601 aagttggaga tttcaccatg tcatcattgt aatcgtcata agtcactaac gattcataag
     3661 cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt ggcgtatgtt
     3721 catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgt ttgaatcttt
     3781 ttttggattc ctgataactt ggataaatca aacctgattc ttcttttgct tttgaataca
     3841 gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg cttacgaaac gtcacaatca
     3901 atccttttgt cttatcagga tacgtacgat tcgtacgtga aaaagcttgg atcaatcctg
     3961 catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca aaacctgtta
     4021 atagacgatc cactacaatc gctaaatcga tttgtttccc aaattgtttg aattccgctc
     4081 tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct gctaatgacc
     4141 aggaagtatc ataatactga ttatattcct caataatttt ttgcatttct tcttgttggg
     4201 cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt ggaaaatcag
     4261 gatcctccat cgttcgtcct tcacgaatga gctgattggg aaactctttt gttaaccaat
     4321 caggctcttt cgtcatctct ttgatggccc gataatactt tttggccatg tcaatggaac
     4381 tcgtggttaa gattgcagat ttcgttggcc tgccatttcg aaaatcaaat tttgtatagg
     4441 cattatctgg acggaacatt ttatgaatga ctttttggat atgttcatct gcttcaaaaa
     4501 cagctgggtc caaataactt tcttttttga caggttccat ttgatcaatc atacgattga
     4561 tctgttctga tgaatactct gcatatgttt ctacttcttt taatttccga tagattttat
     4621 tctctagtga agtcggctcc acggtgtttt catgctctac ttgaaatcct aagacagagc
     4681 catcttctag cgcattctta atggtatagg tatgcaagac ctctccatac tgatcatggg
     4741 ttgtacgtgc caattgccct ttggcttgtt tccgattctc aggaaaaatc ggtgtacctg
     4801 taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata ttttcagcac
     4861 ttaacgctcg atgacactca tctactacaa acacgatatg ttggcccatt aatttttgga
     4921 agcgattggt tccttttttc tcttcttgtt tctttgcata tttcaacgcc gcatctagtt
     4981 tttgacgggt tgtaatgatt acaacatttg cattggcatc tgccaacagg gtttcactta
     5041 attctttggc acttcccgtt ccaacgatca agctattcgc tttggcgttc ccggaagaaa
     5101 tcccagtatt aaattccgaa gcaaattttg taaactctgt ggtggtttga ttatctaaat
     5161 ctttgcggtc cactaacatg atcgttcgat cgatccccga ttttcgtgct aaaagcttag
     5221 tagatacaaa actagtcaac gtctttcctg aaccagtcgc atgccacaca taccctgatt
     5281 gatgtttatt tgcggcaata aacaaggctt gaatcgcatg tacttgataa ggatgtaaca
     5341 ccatgagggt tttattgtct tgatcttcac tgacgattgt ataatccgca atcaaacgat
     5401 gagcatccgg gatatttaac acttgtttac aaaactcata taagttttct actttttcat
     5461 tgtctttcgt tcgccaacta aataaaaact tagggtgcaa gtctttcggt aaggcatttg
     5521 caaaataccg agtggtttgt tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat
     5581 tgttccgaaa cataccttct tctgcatatt ttttgatttg gttaaatgcc tgataaacac
     5641 catcttttgc agttacttgt ttcagctcga tttggacaat cggtaatcca ttgattaata
     5701 aggtgacgtc aaaacgtcga tcccgtgctt ctatgttact tccacgtttt gcaatttgat
     5761 ggaccacttc ataagtagag attcctccac caatgtcttg attggaataa aggataagtg
     5821 agacagaacc caattgcgaa tcttcccgtt caatcgtgat ccgagccatc ccattttcac
     5881 cttttaacca tttcgcagca tcaaatgggg ttttcgttcg cagcagtaat tccgttttga
     5941 tcgtttcaaa ttctttatcg gtcaaaggaa aatcattcag ttctgcttga ttatttgaga
     6001 taattttttg gcgtaaattg acccaaaggt cttcttctga ttttagatcc ggacgatacg
     6061 tccactgatt atgcccttct ccaagtacac gaatcaactg ttcttcgact tctgcttcct
     6121 cacggtgact aattttcatt cttcttccct cctagacaaa catcttttgt aagaagcttt
     6181 ttttcgtttc ttttagtaaa tctaacttac gttgatgaag agcgatcgta tcatctagct
     6241 gtttgaagaa agtaccgatt ttttgttgtt catcctttac aggaacctta acactgatat
     6301 tctcaatatc agaactgtta taagaaggca atgcccctac ttgggagtat cttggcaaat
     6361 ttatagtttc aaacaatatt ttaccaaaat agatatccca agtattatta aataaataag
     6421 ccatggtatt gttatctatt aagaaagggc ttgtgaccaa acgttttcta ttaagcatta
     6481 ttgctgcgcc aactttagca aatataatcg ctggcacatc ttttattggc ttccattttt
     6541 tacgctcaat ttgctcatta gatacataat gatttgcatt tctcatttca tactcattac
     6601 caatgttatt catgtcggaa actttaaaaa atggagtacc ttcagaacca ccttgctcgg
     6661 catcaggaaa tccaattccc gattgagttt tacctaattc tcccaactta cgctcttccc
     6721 aatcttccgt aaaaccagga aaccggattt ctggcacttt tgctccgttt tcagggaaca
     6781 ttttttgtaa gaatcctttt ttcgtttcct tcagcaaatc taacttacgc tgatgaagag
     6841 cgatcgtgtc gtcaagttgt ttgaagaagg tgccgatttt ttgttgttct tcctcactag
     6901 gaaacattgc tactatattt ttaaactttt ttagagacag aacatgttga cctgttccct
     6961 ctgcttgaat agataattgt cgttggattc cattgttaat acgtcttaag aagaagtcgg
     7021 aatcagcaga ttttaaagta aatacaggat aaaaatagct tataatccct ctatcaataa
     7081 tgtcattacg gttaaacaca aacacatcat tatcacttcg actacggaac gtgaaatatc
     7141 ctctaggtag tacaaagtaa ccaatattat tttctgtagt tacttgccga tttgcaaaat
     7201 agtcttcttg caaaactatc cctttttgtt gagatgaagt tagcatagga aattgatttt
     7261 gtacacttgt cttttcaaca taatctacaa cgaagtcccc caacttcctc tgttcccaat
     7321 catctgtaaa tcctgggaaa cggatttctg gttgtgtatc attgctcatg tttgaacacc
     7381 tccagcgcac cttgtaacca cgcttcattt tcaggtgttg tttgcaggga agagatagct
     7441 tctaaaagtt ttttttctag cacttgctgt tcttgtcgta tcttcttgat gtcatttccg
     7501 acatgaacca tatcaattgg ttcttcttct tcgaaagtat ctacatagcg tgggatattt
     7561 aagttatagt cattttcttt gatttcatca aaggttgcca aatgagcgta tttctcgaca
     7621 tcttttcttt cagcgtaggt ttcgaggatt ttttgaatgt tttcttccga tagtttattt
     7681 tgatttttcc ctttaacaaa ttcacgacta gcatcaatga ataatacatc tcgagtttga
     7741 cgattcttct tcaacacaat caccgtcgtt ggaatcgatg tcccaaagaa caagttcgca
     7801 ggcataccga tcacagcata aatactgcca tcttctagta atttttgacg gatgacccct
     7861 tctgcagcgc cacggaataa aactccgtgt ggtaagacga tggccatggt acctgtttcc
     7921 ttcaaatgat agaacccatg aagaagaaac gcaaaatctg ctttggattt tggcgctaat
     7981 ttcccataac gattaaaccg agaatcatct aagaaagtcg tatctgctga ccaattagcc
     8041 gaataaggtg ggttcattac tactgcatca aatgtgtaag gttcatctgt tggccagtcc
     8101 ttattcaacg tatccccatt gcggagattc atttcttcgg cgtcgacacc atgtaaaatc
     8161 aagttcattt ttgctagatt gtatgtcgtt gtatttagtt cttgcccatg atatttaacg
     8221 ttatctgggt gtgttaagta gttacgtacg tttaacatca atgaacctga acccatcgtt
     8281 ggatcaaaaa cactaaagaa tcgtcgttcc ttttgatcga gcgttacgat ttgcgccatc
     8341 atatcagaaa ccatatgagg tgtgtaaaat tctccggctt tcttccctgc ttctgaagca
     8401 aattgactga tcagaaactc atacgcatcc ccgatcacgt ccccatcgtg ttctaaaaca
     8461 tctacatcat tcaatttctt gatgacttcg gtaatcgtca catttcgttg ttgctcatct
     8521 gttcctagtt tttttgattg gagatccaca tcatcaaata acccattaaa ttgattgtag
     8581 gtagatgcca attgcacaaa ggctttgttt agatcgttca attggaacgt tgcttgtttt
     8641 gcttgatccg ctaagacgtt gaaaagatac tctggtgcaa ttgcatatcc caaaatatca
     8701 acgattgtgg caatcagatc ttcttttgat tcttcatctg aaagtaattc acgatacaac
     8761 attgtttgtt tgcttactgt gtcatattcc tctaaggatt catctgctaa aagaaccact
     8821 tgttccaaca atcgatcgga taaatattta tagaaaatca gtcccaataa ataattttta
     8881 tattctgacg catccatttt actacggaga ttatctgccg cactaaataa tttttgattt
     8941 aattctgcac ccaaaaagat acactcctat ctcttattta aagttgctat ataccctctt
     9001 attttagcat accccctttt ataaaaagca aaaaaaaaaa gaaagaaact tattcttgct
     9061 aataaactac gcgaaaggag tctaacgttg tttgatataa cttagactga tcgttaactt
     9121 attttttata tttctacata gttatcttta gaaccatttc agatacatat gaaacaatta
     9181 catatgattt tttcataaaa aaacagtact gtctagtagt ataaaatata caaatttctt
     9241 aaaaattttt tcactaaaat cactacctga aacagtctaa aacacttata tatcaacgtt
     9301 taatgaaact atctccgttt catttcagat acaaataaat atagtcgggc tccaaatccg
     9361 aaaaaaaata cccccagtag cttatcctaa gctactggag atgtataagt acggtcatat
     9421 aacaacgggg tagggtaaat aaatgaaaaa gatggaagaa tcattattca ataacttttt
     9481 taaatacttc gttgtaatca gttacctgtc cgttcttaaa taccacagaa taatcttttt
     9541 tcccattttc aagtaaccga agtggaatgt ctttaccaac ttgctctgtc actgcattac
     9601 tgatacctgt tagattagct aataaatctg ataaagattg agcatctgta gaaatctctg
     9661 tatcagtatt tatcgtgatt cctttatctt catttacaag cttccaagta ggtggtatag
     9721 ctgtgtatgt ttcgatgaat gaatcaattc tttcatctat cgtcttccca gtatctactt
     9781 ctgtctcatt actcttagat gaatcgattc tttcatccat cgtctgctcc gtaactgctt
     9841 ctgtctcatt attctcaccg gaaactctct tcacacccaa acttatatat tgtccatctt
     9901 tagtgtgcat agttaaaatt cccacgcttt tactaataaa ttcactataa ccagttcttt
     9961 caatagagac ttcaatgtat ttcacactat catactcaaa agaaaaatag acatgtaaaa
    10021 gcattttgtt gtttacatct acataataat cctttgactc tacttgagac cttcctatac
    10081 ggaatacagc tttactgtct gatatctcta taccagcatc cttattatta atctgccaat
    10141 ctccaagtag ctcttctggc aagctagtag tttcttgtga acactttacc acttcactgg
    10201 tagaggtcga aacattcacc tccgcactgt tagaagaatt ttccaaggaa tcaggagcct
    10261 tagcacaacc aacaagtctt tatatattgt gcagccgata ttttcttcat tcagtacacc
    10321 cttgaaatat gcagtgttag ataaagcagt tcgtcctgat ttgctgaggc acccatctct
    10381 tcttgaacgt actccttgat tttcaaggcc gcccgatact ctttcggaaa acgctgcttc
    10441 actgaagcca gcaaaaaatc atccttctcc ggcaactctt ccttcttaat cattcgggca
    10501 ataaaaaact gtaaatgcgt cacaaatctt aagtaaagca tcgaatcttt atcaaccttt
    10561 ttttcgggaa acagatatcc tagcatagcc aagatatcct ttagtgctct taccgagagc
    10621 atggtttctt ccatatttgc ttgttcactt tgggcattta caatgtgaaa cgcaatgttt
    10681 ccagcctcct cttctggcaa ctgaacattc atttttttgt tgatcaattg gatagcatag
    10741 cagccgatct caaattcttt tgggtaatac ttttgaattt caatcagcag gcgattttga
    10801 aaaacaatgc ctttttgact gcgttcaatg gcatgcttca agtgatcaac caaggtaaaa
    10861 aatagacttt tattcagctt aaagtcaaac ttggcgttgg cataatcgat aattcgcttg
    10921 acgcaaataa ttagatcatc cggagcagcc tcaaacagtt taatgtaatc cttttttaga
    10981 gcatcagaaa gtgaatctaa tttaaatact ttttggattt tgtcagccgg aaccaaatcg
    11041 cctttcttta gttgaaatcc aatccctttc cccatcaaaa ttacttcttt gccttgctta
    11101 tctaagcaaa gaattaaact gttgtttaac gttttaatta ctctcattag attcaccgtt
    11161 ctctctaagc taagccattt cttgcgatta ctttttgata ccaaaagaag ctttcttttt
    11221 ttattcgttt taattcttta agattctcat cggttcggtc aacatatata aaaccataac
    11281 gcttattgaa gccttgatgg gtactaacaa catcaatcgc tgaccaaggt aaataaccaa
    11341 aaacatctac accatctgtt aaagctgctt gaatagccag aatatgtttg gctagataat
    11401 caattcgatc ctggtcatta attcgtctat cttcctcaac tatgtcgggt gccccatagc
    11461 cattttcagt aattaaaata ggtaaatcat agcgatcgta caatttcctc aatgttaatc
    11521 gtagtccagt agggtcaact acccagccat atggcgtttt atctacataa ggattttcag
    11581 ctgcccggta gacaccttct tctcccaaca tgatctgttg atcccccgct ctagcggaaa
    11641 catctgatcc gtcatttttg ctggcagcga tggtggcagt tgaataataa ttaatcgcaa
    11701 taaaatcggg tttaccggag gataaaattg cctgatcctc tgcctcaatt ttaggatata
    11761 atccacgatc attcaaatag ttttcaaaca aataattgta ttttccccga actgctacat
    11821 ctaagaaaga ccagcctctc aatgtttccc aattatgggc tgcaattgca tcttctggac
    11881 tggaggtagc ttgatacata gccgttaaat tgattgctgg accgattttt cctttaagat
    11941 tcaactgatg atacaagtga attactctag cttgagctaa taaaacatgg tgattaattt
    12001 gaaagagttc tttttttgaa ggcagctctc cacttggtgg caaaccaatc gctccaggat
    12061 gaaggatcat cgtgttttgc tcattaattg tcagccaata gttcaccttg tcaccatact
    12121 cagtaagcaa tacctgtaca tagtccacaa aataatcgat agttgctcga tttaaccaac
    12181 cgccctgctc ctctagaaaa taaggtaaat cgaaatggta catggtgaca atcggttcga
    12241 ttccctgatt caggcattca tcaattactt gatgataaaa agaaattccc ttttgattgg
    12301 ttttattatt ttcaatatcc ataattcgcg tccaagcgat ggaaaagcga aaagctttta
    12361 agcctaattc tttaaacagc tgtatatctt ctttatagtg atggtaaaaa tccgaggcaa
    12421 cttcaaaatt agctacccct tctgtatgct gatacttgtc aataatagac ggttgtttcc
    12481 catcctcctg ataggcaccc tctgcctggt aggcactaat ggaggcaccc cataaaaact
    12541 tctctggaaa tgctgaaagc tgtttgtatt gcacaaaaat cctctcctta actaatttgc
    12601 atcaatggtt cgtgttcctt taagcttccg cttgcttcac taattcctgc gtaattttca
    12661 ctgttggtca ctaccacaat cactgtatca tcgtagccag cgtttctaat ggcagcgaaa
    12721 tcggcgttga ttaataattg tcctgctgac acctgatctc ctgcttttac aaatacttca
    12781 aagggttccc cttttaaatt cacggtgtcg atccccacat ggatcaataa ttcaatacct
    12841 tctttagaac gtagaccaat cgcatgttta gattctaaaa tagtaatgac ttctccctca
    12901 acaggtgaat aaatgctgtt ttcttctgga tgaatggcta cgccctttcc caatagtccc
    12961 ttagaaaaga cagcatcgtt cacttcagaa ataggacaca cggttccttt taccggattt
    13021 tcaacaatga ctgcttcggc tattgccgtt tgctctgatt catcagaagt atcgtcaaat
    13081 ccaattagat aagcaatgat aaaggttgca acaaaggcga ttacacacgt aataattgcg
    13141 tgaacaatat tcattggatt ttctccaata aaggctggta aggctgctaa gccaggggaa
    13201 acaaaggcat atctgactaa accgctcaag ccagcataca aaccagcaat tgcaccacca
    13261 atcatggaag caactaatgg ttttttcagc ttcaaggtta ctccatagag tgaaggctca
    13321 gtaattccca ttaaagcagt gatcccagtg gacacagcta gctgcttcaa ctctttgttt
    13381 ttagttttcg cggctacagc aaaggtagca cctgattgag caatattaga agccagcatt
    13441 cctggcccat tgatcatttc agaacccgta ctgctcaatt gcatagtagc gattggcgtc
    13501 attgcccaag ctgtacccgt aaccaccaaa aatggctgca aaccacccat caaaaatgga
    13561 attaaccaag ctgctttgcc gttaatccat tcagccccca ttgctataag atcgttcagc
    13621 aacgttccaa acggtccaat gacaaccaac gctaaaggcc ccataattaa aaataccagc
    13681 atcggcttag taaagaattt aataaacgaa ggcgaaattc gatccgcaaa gggctctaca
    13741 taggacatga tccaaacagt aaagataatc ggtaaaactg atcccgcata atctgcagct
    13801 aataccggaa ttcctagaaa agaaacggca tcgccagtag aaaaaagctg tgaaatattt
    13861 ggatgaagca aaatgccagc aacagtaatc gccaaaattg ggttgacatc aaactttatg
    13921 gctgccccat aggctaatag tactggcata aaataaaagg ctgcatcagc aatagtattc
    13981 aaaatgatgt aagtttgact actgtctgat agtacaccag tcaaagtcaa gacggccaaa
    14041 atggctttta ccatcccggc acctgttata gctggaatca tcggtgtaaa ggtggtagaa
    14101 atgacactaa tcaatcttga aataatactt tcttgattgt ctgaagaggg gcccttttta
    14161 attgaagttc ccacttcctt ttgaatttcc ttgaaggctg tttggacttc attgccaatc
    14221 accacttggt attgtccacc gctatccacc acactaatta caccaagtgt tttttctaag
    14281 gctttcgtat cggctttcct tttatctttg agttcaaaac gcagtctggt ggcacaatgg
    14341 gttaagctga ccacattttc ttttccccca acctgttgca atacatttct tcctacttct
    14401 ctgtaatcca ttattctctc tccttaaagc agtaaaaaag accaagtcaa accacgcccc
    14461 aatcaacatt gaagtgttag tttgtcttgg tcttgcctgc ataatcagta acatgccaat
    14521 aacgttattt tgctgtaatc caataatagc acattatgat agcgcaatca acagggaatt
    14581 taatttttta tcttttcgtt tgaccgtata gggtaatata acagttagat cacatcaact
    14641 agggtatatc tgcgtatact tactgctaaa ataagtatat ctaatattaa ctataccagt
    14701 aaaaccattc ctacataacc agcgggaaat tcccaaaatc gtgaaagcat caaaaatgca
    14761 tataacggat cacacaactt caacaattct gctcttggga aaaattgatg aatttggtga
    14821 gtaaaggatg gaataaacag cattccaaca atataggcaa caatagtgat acttgttttt
    14881 ttggtaaaaa tcagtaagca atagccaatt tcaatcattc cgagatataa aggtatcgat
    14941 agaatgattt gggctagtat tcttaaaaat taactagcaa atgtttaaac tgatttattt
    15001 ctaatctaca tgtagtaagt atttcaattc caccaattat caataaaaat accaaacata
    15061 tccataataa ttttttcttt ttcatttctt ttatattgtt ataacagtga ataacataaa
    15121 aattcccgac aatcattgta atattttttt gtcacctgta ttagtattta atttatagga
    15181 aactataaaa actccctagt gaatttacac ctttgggagt tcctgtttgg acggtatgca
    15241 taatacattt aaatcaattt aacattaaca cctcagcata tggtatctcc acatatatac
    15301 ggtctttctt tggatagtac tcaatattta tgttgatagc acttccattg tctaatatag
    15361 ctttagcccg gtatgtgtta tctccatttt catgagtaat cgtcacatca tcacatgatc
    15421 gatttaagtt gtactgttct tgtaaaatct gtgttaccaa tggttttgat gacttctcta
    15481 atccagatgt acctgtcatt tttgaaaaaa taatacctat aatagccaag acaacaatac
    15541 ctataatgac aaacttcttg ttcatattta ctttattcaa ctttttatct cctatctcca
    15601 aaataatttg ctcttatccc tcttatcagt acattgacat ccctaaaagt acaccaaaga
    15661 ttgctccagc tacccctgct atcatcaaaa tcgtacccat tttctcatct ttttctcgat
    15721 ataagtagcc cataataaca gcaacagcac caaatacaac aggaataaat gctaacgata
    15781 ctaccataga aatccaacca ataatcaaat acagcttccc tgtctcttgt ccatgtacga
    15841 tttgttcctt catttcatta attgtctgct cagtactttt ctttactgtg tctgttccgg
    15901 ttgtctcatc tgtttcttgt agtgctccac aaaactggca atacttactt tcatctgtta
    15961 tttctttgcc acattttcta caatacatgt cttctcctcc taaaatcatt caattgctta
    16021 ttagttaact gtttacttta ttaaatacat cagaaaacag ctcattcatt ggtacactta
    16081 atatctcaga aagcatcttc atttgatagg gtttaggaat cgttctccct gtttgccacg
    16141 aacttacaca ttgctgtgta acattcattt tttttgctaa ctccgtttga gaaagtcctt
    16201 tttcttttct gtatcggctg agattactta cttttttcat gattctcccc tcctgtttta
    16261 ttatatacaa caatatgatg tatttcaact cataaaatat aaaaaacttc aaataccatt
    16321 acaaatacaa caacttgttg tactatactg agatgggggt gtattaatga aacagaaaca
    16381 atatgcaaag gtcattagag aaaagcgaaa agctctcggg tggacacaaa aagaattagc
    16441 tgaaaaaatc ttctctacac aacaggcagt agcaagatgg gaaaattctg taacagagcc
    16501 taacctcgat agcttaacag ctctttctag agccctagga acccctgtaa gccattttct
    16561 agacaatgtt gtcgtagatt atgaggaaga gtttttggct ctctatcgtt ctcttagcac
    16621 tgaagatgca gttcgaacaa ttgactatat gaagctatta aaaaggcaag aaaatgaacg
    16681 caatcagcta ttgaaagatt gaaaaataaa taacccaccg aactttaacg gtgggtgtat
    16741 tgagatgaaa taagattaag agctatggat aacctgtcta gtttaataca attgggtctt
    16801 ttctcttcct tactctccgt ccatcatctc ttgttacact tgtaaattga tacagaggaa
    16861 ctatcccagc tcctgataat atcaattctt ttattgtttc atctatccct gcccctttcg
    16921 ctaaatatat taaaaaacta ctacttgctt tcacattgta tagtttctta atatccttag
    16981 aagctcgttc aagagtgaat ccttgtttaa caaagtagcc tctgtttttt attaaagcat
    17041 ttttcacgct gttatcccca acattacgta ggcgttcaca aacagtgtta ataagttctt
    17101 tctctgtcat tagatatgtc ccttgccacg ataagccctg taaaacttca tgtgcttcat
    17161 ctaaaatcaa ttgtttttcc ttgctgagtt ctgatcggga attttgagga aatactcttt
    17221 tagcctcttt ttcgttaaaa catatcttaa cccattcata agaaaaatat tgatatgtaa
    17281 aatgaatttt ttccagaagt ggaaggatat cttcattcaa tttttgaatc acagaagttg
    17341 cattaaaaac gtctaatcga tagacattgc ttctcttatg attaaagttt cctaacgctt
    17401 tctcttcctt taacctattt aggtattcga gcatcttcaa aggaacatct tcatcatcta
    17461 atttttttat caatcctata aaagtcataa gtgatagtaa cttattacat ttatctatgt
    17521 cataagtttt accatagtaa gaactcattt ttttagctaa ttgttccccc gacaaaatac
    17581 aagtcacact ttccttacta tcatcaaaaa aattaacatc agaaagcaag acatcataga
    17641 cacgtcgcat ttcctctaga taattcctta ttgtcctgaa aagtttgtct ttcattgatt
    17701 caagagtgtc aaaaagtact tttgattcct ctttcaattt tcgaaaatgt gtaggataca
    17761 agtctaggtt ctctaatagc caattaaatg tttctacccg actgtctttc tttaaaatta
    17821 gcatcacaac tccaataaaa ttttcactga tatcacaatt actggaatga caataaaaaa
    17881 tggaatgccc agttttactg gtaaaaatac tagcagaggg attatgatcg ttgtgaaaca
    17941 gacatcttaa gtttttactt gacttaagta atttattcat atcaatggtt aacaatcttt
    18001 cataaatctc attaaaagac atgtcggagg aatcaaacag gcagtcttta aaccactgtt
    18061 tcatttcttc tttgtcgtca ttttttatca acttaacaaa atcggtttca ttgactagtt
    18121 catactctct ccctgaatca ctttgacaat ttttagaaca tgtcatggta aaatcaatcg
    18181 gaagctcagt aatatctaac atgttgtctt cattaattat tatcactgca tctgacttat
    18241 atcctccaaa aaacattcta ttcgaagcat ttacattgtc atcacacccg gggatgttct
    18301 ttttcaagta ttcgtaaaca gcaaccattt taccatagtc agtaattgct tcttttaata
    18361 gaaagacaat cttgtatttt tctagatttt cagagtaccg aatacttttt tgtatcgcaa
    18421 acgcagaatt atgaactaaa ttgatagata aaaccttttc tacgcttaag tatctactat
    18481 catctttact caaatctact accttcccct tctcatcttt aacagtgtta tcgacatcca
    18541 gaataattac tctggtttcc acaaaactct ccttgtcatt gcctttattc tttgtcttgc
    18601 ttggaataaa tgattgtgat gaatttagta tctctacaaa ctcatctctt gtaacttctc
    18661 tatagttatc taaacaccta tctcttacat cagttttttc tttttttgta actggcttct
    18721 ctaataattt ttcttgatct acataaattg gaatattaat cattgatgtt tccatttgtg
    18781 tttcctcctt ataaaattct gttaaagact aaaacataca gataataaaa acaaactctt
    18841 tattactata tgttttagtc ttatttgttt actcaacctt tataccgttc catgaaacta
    18901 tcaatatcag attttttaaa tctaatgacc gtatcaattt ttataacagg taacccgtat
    18961 tgagaaacat attttctaat cgttcctact gatacaccta gatattcact tgcctgctga
    19021 tagtccatat actctagagt gactccagaa ataatgtctt ctagcatagg ctttagcact
    19081 gtaattatct ctttcacttg ttgttcaact tgtatagaaa caatgctatc aatgatttgt
    19141 agagacatat ttccccctcc tttcgtgatt gcttgaacaa attttctaag aattatcttc
    19201 gtattttttg ctcaaaataa aagtattttg ttattaaccg attaatcaaa caaaaaatga
    19261 ttaccagttc atcgacatac acataatacc tcgataaaaa aggggtgtca acatctttgt
    19321 aaaatttatt tacaacaccc cttttttgag gtatactaat tttaggaggt gtggtcaata
    19381 tgataaaacg tccaccaata aattatttag aacgaaaaaa gatactcgga acaaaaataa
    19441 aagctataag aaagagtaaa aaattaacac aaccagcatt tggtcttatg attaacaacg
    19501 gtcaattaat tgacaaaaaa actatctacg aatgggagaa gggaacctat ttaccaatac
    19561 cagagcgatt atcccgaatt gctgatttgg gaaatatgag tatagaggag ttagtttgtg
    19621 gcaatgtaga agaatatatt ttgggaatta ttctttatag agatagcatt gtactagatg
    19681 gtataacatt tccagataaa aacctgttcc agcatcttcg tcaacagttc ccaccagtac
    19741 actctaattt agatacatgg ctggatagat attccaaatt agaaccagag atgcaagaat
    19801 ttattgctaa caagacttgt aataaagtta aaaatgaaaa aattagtctt ttcaatatct
    19861 taaaaataga agaattattc atcaatgcta ttgtagaaaa atttgacaat aacattcttt
    19921 tcctcactag cagtatagaa gaactactag aaagaatggt agatgaatgg ctacctattc
    19981 aattgaaaga catgtcttat cccgaagaag ctgtgagaga aattactgat aatataaaca
    20041 agctagaaca aacgatttca tcaattggga aaaaatacac taagaaaaaa atgaagggag
    20101 gtgataccat ctagtggtaa gtactccttg taaactaaga ggagatgatt taatggcaaa
    20161 aatatcaaag tacaaaaaga aaaacggtca agtggcgtgg atgtataaag gacacattgc
    20221 caccgatcca cgaactggtg aaaaaataaa tactacgaga agaggtttta gtagcaaaca
    20281 agaagctcaa aaagattatg acgagtacag acatcaaata ttatacggtg ttaaaaagaa
    20341 agcacctgat atgacatttg aagacctgta taatgaatgg ataactcatc agcgtaccag
    20401 tgttaaagct tcaacaatcg caatctcagt aagatacgca aataatcaaa tacttccagc
    20461 atttggtaag cttaaactat cgaatatctc tgtcccatat tgtcaaaaag tggtagatga
    20521 atggcacagt aaatatgaaa gttacgatta tatgagaaaa cagactgcac aaattttacg
    20581 ctacggggtt gcaatgcaat atattgacaa taatcctatg gaaaaaacac ttcttccacg
    20641 aaaaaaagag tatgagaaaa atcgtaaatt ttactcaaaa gaggagctta ataatttact
    20701 ggatgctttt aaagattttg gtaacatgaa acaatacgcc ttcttcagat tattggcata
    20761 cacaggtatg agaaagagtg aagtactcgc tttacagtgg aaagacatag acacgtttaa
    20821 taaagaacta catgtcaata aaactcttgc tgtagatgaa tttggtaaag ttataattca
    20881 atctccgaaa acaagagctt ctagacgagt tatctcttta gatactgaga ctctttctat
    20941 cctcaacaac tggaaattac agcaaaaaga agagtattta aaacttggat acaatactag
    21001 ctctaaagag caacatgttt ttacaacagt taaaaacact ctatacattc caaacactgt
    21061 taatgattgg ttaagatata ttttgaaaaa atataacttg cctcgtatta cacctcatgg
    21121 gtttagacat acacatgcta gtttattatt agaagcagga gaatctgtca aggtagttca
    21181 acaacgatta gggcatgaaa atagtaaagt gacattagac atctatgcac atattacaaa
    21241 taatgcacct aagaaaacag gtcaagactt tgctgatatg atggctcatc agtaaaagag
    21301 ctggtcaaaa tctggtcatt tctagtatac aggcaaaaca aaaaagctag aacccttata
    21361 tatcaagggt tctagctcct ataacaggtt tacccaacac tgccttccat ctcataactg
    21421 atcaatcgat tgagttcgac tgcatattcc attggaagtt ctttggtgaa tggttcaaca
    21481 aagcccatga cgatcatttc agttgcttca gattcggtca ggcctcggct catcagatag
    21541 tagagttgtt cttctgagat ttttgagact ttggcttcgt gttctaaaga gacttggctg
    21601 ttgtggatct cattgaatgg gatcgtatcg gatttcgacc attcgtccat gatgatcgtg
    21661 tcacattcga tatgagagat cgaacccgca ctgtttttgg caaaggttac ttgtcctcga
    21721 tagtttactt ctccgccgtc ttttgaaatt gatttagaga cgatagagct agatgtattt
    21781 ggtgcattgt ggatcatttt tgcacccgta tcttggattt ggtttgctcc tgcaaaggcg
    21841 atggagagca tggtgcctcg tgcacctttt ccatctaagt aaacacttgg gtatttcatc
    21901 gttgtttttg cacctaggtt tccgtcgatc cattcaacgg ttgcgccttc gtatgctttt
    21961 gctcgtttgg ttactaagtt gtacacattg tctgaccagt tttggatcgt tgtgtaacgg
    22021 caatacgcat cttttcttgt aaagatttct acgatggcgg catgcaggct gttacttgaa
    22081 taagtaggtg ccgtacaacc ttcgacatag tggatgcttg ctccttcgtc aacaatgatc
    22141 agcgtacgtt cgaattgccc cgtgttttcc gcattgatcc ggaagtacgt ttgtaatgga
    22201 acatcgactt tgacggcttt tggcacgtaa ataaaggtcc cacctgacca tacggctgaa
    22261 ttcagcgctg ctaatttgtt gtcggttggc ggtacaagtt ttgaaaagta ctctttgaat
    22321 aattcagggt attctttcaa agcagagtca gtgtcagtaa agacgattcc taacttttcg
    22381 aattcatctt tcatattatg gtacacgact tctgattcat attgtgcgga agcaccggct
    22441 agataagctc gttcagcttc tggtatacca atcttttcaa atgtatcttt gattttttct
    22501 ggtacgtctt cccagtcacg agcgggtttg tcacttggtt tttggtaata tttgatggca
    22561 tcgaaatcga tgtctgaaag gtcggggccc catgtttgca ttggcatttt gttaaatgtt
    22621 tctagagatt tcagacggaa ttcaagcatc cattctggtt cgcctttgat tcttgacatc
    22681 tcacggatca cttcttctgt caagccttct cctgtactga aaaccggttc gacatcatcg
    22741 tgaaacccga atttatattc ttctaattct ggtacgccca tgttctcact cctcttcctt
    22801 gtggtaatga tggaaagctg cggtaccatc gttttccatt gcttttccta gtgctttcca
    22861 ggcaagtgtc gcacatttga tccgagcggg aaatttagct acaccaccaa gcatagcagc
    22921 atcgcctaat tggtcgggat ctttgacttc ttttccttgg acaagctgta gaaagtctac
    22981 agttaaatgt tctgcttctt ctattgattt tccgatgacg gcgtctgtca tcatcgaggc
    23041 acttgccgta ctgatggagc agccactgcc gtcaaaggcg atatttttga tgattccgtc
    23101 ttttacatcg acttgcaatt cgatcacatc tccacatgtg gggttgttca attcgatctt
    23161 gcttgtcgag tcagtcaagc ttccacgatg atgcggatgg gaagaatgat ccaagatcac
    23221 ttgacggtat aaattgtcta atttagatag tcccatgttg gaaaaactcc tttgttgcca
    23281 tgattgcttc aactaaacgg tcggcgtctt gcgttgtgtt gtagaaataa aagctcgcgc
    23341 gagcagtcgc tgatacttca aggtacttca atagcggttg cgcacaatga tgacctgctc
    23401 tgacggctac cccttccatg tctaaagctg tcgcgacatc atggggatgt aacccatcca
    23461 aattgaatgc aacgactcct gtccgaaaat tcgggtcttg tggaccataa acagtcaatc
    23521 catcaattgc ttgtaatttt ggcaacacat aagcggctag ttcttcttca tatcgatgga
    23581 tattgtccat accgagtttt tgcaaataat cgattgcatg gcctaatgcg atagcgccgg
    23641 cgatatttgg tgtccctgct tcgaatttcc aaggtagttc tttccatgta ctgtcgtata
    23701 attcgacaaa atcgatcatt tcgccaccaa attcaacagg ttccatttct tctaaccatt
    23761 ttcgtttacc atataaaaca ccaatacctg ttggtccgca cattttgtgt ccgctgaaag
    23821 caaagaaatc acagtctagt gcttggacat caatggccat gtgcggggca gattgagcac
    23881 catctactac gatgaccccg ccgttttcat ggacaagttc agctaattct tgaacaggat
    23941 tgaagacacc taagacattg gatacatgag caatggagac gattttggtt ttgtccgtaa
    24001 tctttttcgc tgcatcctcc atatccagga agccttcttc tgttagttcg atatatttca
    24061 agacagcacc tgttcgtttt gccaactgct gccacgggat aacattagaa tgatgttcca
    24121 tgtaagagag cactatctcg tctcctgctt tgatatagcg gtcgccgaag gttctggcta
    24181 cccaattcaa gcttgtcgtc gtaccacgag taaacagcac ttctgctgtt tcttttgcat
    24241 ggatgaacgc ccgtactttt tctcttgctt gttcataatc atgggtcgcg cgttctgcta
    24301 acgtatggac accgcgatga acattggcgt tatcgtgttc gtaatagtac gctaatgcct
    24361 ggagtacttg ttttggcttt tgggtcgtcg ctgcattatc cagataaact aaaggttcat
    24421 cattgacgtc ttgaaataag ataggaaaat cattgcgtag ctttttagca tcgatcatcc
    24481 attcaacttc ccttcgatta cttcgactaa ttctttccga acttgttcta cagggattgc
    24541 tgttaagaca gaaccgagga atcctcgaat cactaagcgc tctgcttctt ctttatgcaa
    24601 tcctctactc atcagatagt acatttcttc tggatcaact cgtcctacac tggcagcatg
    24661 tccagcagtt acttcgtttt catcgatcaa aagaatagga ttcgcatcac cacgtgcttt
    24721 atcagaaagc atcaagacac gactttcttg ttgggcatct gcaccttttg cccctttcaa
    24781 gatgtggccg ataccattaa aggtcaatgt tcctttttct cggatcactc cgtgttgcaa
    24841 aatatgcccg atcgtatgag gggctttatt ggttactcgt gtatcgattc cttggatttg
    24901 tctgcccgag ctgattgcca cgattttgac ttctgcgtgt gcaccttctc ctgctaaatc
    24961 tgagtcaaag tcagcgatca catgaccgtc attcatgaca ccaattgccc agtcgacaga
    25021 agcatctcgt aaaatgtgcc cacggcgatt catataagaa gtaatatttt ctcctaattg
    25081 atctacggct gaatatttga tttttgaacc agctttagcg atgacttcca cgatgatatt
    25141 acctgatgat tttgctactt gttctttggt tgtctggaaa cgttccagat aagaaaattc
    25201 actatgttca tctgccacga tcaacacatg cttgaagaag tgctcatcgg atgcgccatc
    25261 ttgaatgaat aaggactcga gtggttcatc gatcacgaca tttttaggca catacaggaa
    25321 aataccgctg ttcataaaag ctacatggag tgcagtcaat tggtcttcgt tcatgtttac
    25381 tgcttttttc atataatatt cttggactag ttctggatat tcgatcattg cagtgaacaa
    25441 gtcggtaaaa atgactcctt tttctgctaa ttccacaggc agttgttcaa aaatcgtcca
    25501 tgaaccttgt tggacaagca caggattgtc tttcattgca tcaaatgccg gaatcgtgcc
    25561 tgtttcactg atggtttgtg tttctttcac atcaaataac gaccagcgat ggaatttcac
    25621 ccgatcgata tgcggcaatg gcagttcatc tgctttcgct agtgcgtctt gacggaaggt
    25681 tctcatccaa tcaggttcgc ctttacttaa agaaaaatca tttaccgcgt caagataatc
    25741 gagccagttt ttttctttca ttcttgctcc cccttacgct tcttcttctt tatagtcgat
    25801 tcctaattct ttactgatac cagcgtagcc ttctgcttcc agacgtttag ctaggtcagc
    25861 attccctgtc atgactactc ggccttccat catgatgtga acgacatcag gtgtgatata
    25921 gttcaataat cgttgatagt gggtgatgat caatgcaccg aaattgtctc cgcgcatttc
    25981 atttactcct ttagcaacga ctttcaaagc atcgatatcc agacctgaat cgatttcgtc
    26041 taaaattgca aatgtaggtt ctaacatcaa caattgcagg atctcattcc gtttcttttc
    26101 tccgccagaa aaaccttcgt tcagataacg ttcagccatt tcttctggca tatttagtaa
    26161 agccattttt tcatctaact ttttcaaaaa tgccataaca gaaattttgt cgttttcatc
    26221 acgtttcgca ttgatcgctg cacgcatgaa ttccgcattc gtgatacctg gtatctcact
    26281 tggatattgc atagctaaaa ataagccaag tcgcgcacgt tcatctactt ccaagtctaa
    26341 aacatttttc ccgtcaaaca aaatttcgcc ttctgttact tcatagtttg gattgcccat
    26401 gatagcggca gataaggtag attttcctgt gccgttaggt cccatgatgg catggatttc
    26461 tccggttttc attgttaggt ttacgccttt aaggatttct ttttcctcga tcgacacatg
    26521 tagatttttt atttctaaga cagacatgca ttataccccc aaattctctt tcgttctcac
    26581 tcattttaga ctaattgaga atgaagcgca atgaccttcc ttactaaaat caaatatctt
    26641 ttatttatta ttaaacaatt ttgatttcta aattagaata ggtataaaaa agatgagaaa
    26701 taagccatta cagacttatt tctcatcttt tttgttccat accttatttt gcatcagcct
    26761 ttactggttt aacagagccg ccccatttgt ctgtgatcca ttcttggact tctttgcggt
    26821 gaagcacttc aatcagtttt ttgattttgt cactgtcttc atcaccttta cgagtcgcaa
    26881 taatattcac gtaaggagag ttgtcctttt caagcaatac ggcatcttct aatggattca
    26941 accctgcgcc gtaagcgaag tttgcgttga ttgcaactag gtcaccttca tcattttggt
    27001 acgtagaagc taaaagtgat gggtcgatcg tatggttgaa cttcaagttc ttagggtttt
    27061 cttcaatatc atcgaatgtt gctgtttcca gatctactcc atctttgact ttgactaagc
    27121 cagcatcttg tagaattgtg atgatgcgtc cccagtctga ttcagaattt gaagtaatga
    27181 ttgttgcacc atcttttaat tcggaaacat ctttgacttt tttagagtac aagcccattg
    27241 gttcgatgtg gacagcaccg gcattgacaa agtcatatcc tttttctttg atttctttgt
    27301 tcaaataagg aatatgctgg aagtaattgg catcaatgtc gccttcttcc aaagattggt
    27361 tcggtaatac atagtcatcg aatttgacga tttcaagatc gattccttca tctttcaata
    27421 atggtttcac atgttccaaa atctctgcgt gcggtgtcgg tgaagcgccg actttcaatg
    27481 tcgtgtcgtc tgctttattt gctgaatctt tgctgtcgct gttgttaccg catgctgcta
    27541 atcctacagt cagcaaaagc gctgctgcaa atccaaaaat tttctttttc atatcctaca
    27601 tccccctata tcctattatc ttttgtctgt tttcttagtt aagaaatcac cgattccttg
    27661 aacgataaat acgattagta aaataattaa agtcgcaacg aatgtcactg ttagattacc
    27721 acgctggtag ccttcctgcc aagcaagacc tccaagtcca cctgcgccga tcgcgccggc
    27781 catcgcagta aatccgatca tcgagatcgt tgtaactgtt aatccagaaa tcaatgcggg
    27841 aatactttca ggtatcaaga ccttccagat gatttcccag taacttgctc ccattgcatc
    27901 tgctgcttcc agtacaccag ggctgacttc acgaaaagca atctctacta gacgggcata
    27961 aaatggtgct gcggataaaa caagagctgg aatggctgct tgcgcaccta gcatagtgcc
    28021 tacgattgct ttagtaaatg gaatgatcaa aaccatcagg atcataaatg gcgttgaccg
    28081 gaaaatatta ctgatgatcg atacgattcc ataaatgatt cgagcatacg gtttgttttt
    28141 gcgtccaata gagtaaagga tcaatcccaa aactaatccg atgatcgtca ctaagaccat
    28201 tgagattaat gtcatatata gtgtatcaag gatagcctga ttcatcgttt ccgggttgat
    28261 ttgtttaaaa tctagatatg tttctgaaaa gcttttatcc acgatggatc acctcaattc
    28321 ctactccttg ttctttgatg aacacttctg cttgatctaa ttctttggtt tcaccagtca
    28381 tcaagacagt caatgaacca aaggaacctt ctttcacttg tttgatcttt ccatacacaa
    28441 cgttgacatc gatgttatag cgtcgaatcg cttgggaaat gactggctcg ttggcatttc
    28501 tttcgctaaa ggtcaaagtt gccaaacggc cgcttggatt ttctttgatg agttcagcta
    28561 atagttctgt cgaatcagct tctggttcga tgtcttgttg tacaaagcgt ttggttactt
    28621 cttttttcgg ttgtctgaag acagtcaata cgtctccttc ttctacgact ttccccaact
    28681 ccatcacagc gactttgttg cagattttgc ggatcacatt catttcatgt gtgatcaaga
    28741 cgatggtcaa attcaatttc ttattgatcg ctaacaataa gtctaatact tcttctgttg
    28801 tttgcgggtc caatgcgcta gtagcctcat cacaaagcag gatctcagga tcatttgcca
    28861 atgctcgtgc gataccgacg cgttgtttct gtccgccgga aagttgtgaa ggatatgctt
    28921 gtccgcgtcc ttctaacccg actaaacgca acagttcttc tgcccgttct ttacgtttgc
    28981 tctttggtac gccagctaat tccagcggca gctggatatt ttcaagtaca gttcttgacc
    29041 ataacaggtt gaaatgctgg aagatcatac cgattttctt tcggaatgta cgtaattctt
    29101 tgttttttaa ttttgtaata tcttgatcgt ttacgatcac ttcgccgctc gtgggaagct
    29161 caagtccatt caggagacgg actaatgtac tttttcctgc gccagaatag ccgacgattc
    29221 cataaatatc ccctttttca acgtttagtg aaacgtcatt gacggcttgg atgtttccgt
    29281 ttttcccatt gaatgtcttt ttgacattcg ttagttggat taatgacatg ccattcacat
    29341 ctttctctta tgatttttca aacaaaaaag ccttcgccct cttgccttaa gcaaaaggac
    29401 gaaagcttga acttccgtgt taccaccttg atttaccgtt acttcgcaat aacagcctct
    29461 tccagtactt ggacttagct tctttccgta tactgtcgcg ctgtaacggg cactcccgta
    29521 gtaagtttgc ttacgctcgc ttactctgct ctaagaccat cttccatctc attttcgtta
    29581 cccattttca gcaaacaggg ctctcttcaa acgtccgtga catgtactct tcttttcaaa
    29641 gctttctcgt ttcatatagg attgcttatg attatagcaa ctggaagaat cagtgtcaac
    29701 ttttttcaag tgattttctg attttataat tcgtcaaatt cttttggatc aacatctttg
    29761 aaacttaaga tccacgcatt catttcatct gaatcattca agatattgat atcttcaccg
    29821 attttatcat taacagaaga aactgtaccc gttagcgggc tggcaaattc attgaccgct
    29881 ttttccgctt caacttcgat cagaggttca tcttttttta gcgattgtcc tgcttttggc
    29941 agattggcaa acgtgatgtc tccaagttct tcttgcgctt catttgtcaa accaacacag
    30001 tattcttttc cattaaataa gatccaaagg ttgtcctttt tcttcaaatg cttgcctgcc
    30061 attttatatc agttctcctt cataaatttt ttcattgaat ccaattgcaa caagctggtc
    30121 ctctcgtaag atcagtggtc gcttgatcag cataccatct gtcgataaga cttggctcgc
    30181 ttttgaagcc gtaaactcgc ctacttgatc tttcagtcca agttcccgat atttcatccc
    30241 gcttgtgttg aacaggcgac ggattggtac ttgactcgtt tctagccaag tctcaatcgt
    30301 tgaggccttc ggtacttctt tgatcatatc aacggtttga taactaacgc catgctggtc
    30361 taaccacgct ttggcttttt tgcatgttga acatttcggg taccagtaaa aggtgtacaa
    30421 ttttcttacc tccttgaaga tcttgcaggt gcttcatttt ggtatctcat cgataatatc
    30481 aacccgatac cgatcatgtt ccctaaaatc gaggatcctc cctgactaat gaaaggcaat
    30541 gggatccctg ttaaaggaag taatccgata tttgctccaa tattctcaaa gacatggaac
    30601 agcagcatca tgatcaaacc gcttgcgata taggcgtaaa attcattatt cgtatcgaaa
    30661 cacacccgaa tcatacgata gattaaaata aaatacaaga aaatgacgaa tgcaccaccg
    30721 ataaagccga agttttcacc gataacggta aaaatcatat cggactcacg aacaggcaca
    30781 tagatattgc ttacgttgaa tcctttacca aacaagccac cggtaccaat tgccaaaagt
    30841 ccttgggccg gctgataact cattcctgac gtatcatgga aagggtccag ccatgaatca
    30901 atacgtgcaa actgataaga tttgaatcca actttataaa gcaagtctct tccgccttca
    30961 gtcgtaacca agaagatcgt tcctgctcca acgactactg ccaagattac tacaggtgcg
    31021 atgatgcgcc aagagattcc agacatcagg aaaatcccgc caaagatcgc aagaaagaca
    31081 agcatcgttc caaagtcatc ttgggccatg atcaatgcta aaaccggtat tgtcacgatc
    31141 aagagcttcc caatcagcca aaagtccgat ttcatcgtcc gttcttttac ttgcgtatta
    31201 tgtttagtta cgatcagtgc catcatcaag atgtacgcaa tcttcatcaa ctctgctggc
    31261 tggaaagtaa acgcaccgaa tcgaaaccag tttttagacc ctgttgaagc agctaaattc
    31321 tgatcataga atttgagcaa gagtcccatt accactaacc ccaaaccata aaggtaagga
    31381 gtcagtttcc agatccattt ggaattcaaa tgcatgatga caacgattgc tgccgctcca
    31441 actaaatacc agacaacttg catccctacg cctttcaaga ctgctggatt gttcggatca
    31501 tggctaagtg ctacatataa tgagagcaga ccaatcaaac ataacagaaa gactggtaaa
    31561 atcacgccat aatcaatacg attatcgttc gaagctttta attttttttc catgtttcat
    31621 cccttttctt aaaaaccata ctgctgatta ttatagcggt ccagttagat aatggaaagt
    31681 ctttccatag acatttggga aaaattaact ttccgtttgt gcagttgctt gagcaaatgc
    31741 agtacaaaag cctactgctt atctttagtg tatcatagaa ctttttttga tacatgcaat
    31801 tatctaccag ctgagattct cctcgctgtg agaagtttct gtgctttcat tgtatcgaag
    31861 gatctcttcg gcaatctcta gcagctgatc agaaaccaga ttggttcgtt ggatgaaata
    31921 aaaatatcga tggaaattca acggagtcat tggcacattt tctggaacag agcgcttaga
    31981 caaaatcgag cgaccgattc cttccttcag caacgcaaca attacatcgt tacttttcac
    32041 gatcatcttc ttatcttgga tattcatctc ttcaaaataa cgctgcgtgt aatgaaaaac
    32101 accagagttt gcttctctta caagccacaa tttgcttttt gggtccccag cgatgactag
    32161 ctggtcttcc atcaaagagt atctttgtac gccggaagtc tctaatggct tttcgataaa
    32221 accaaaatcg gattcatgtt tcgctacttg ttctaatgat tcatgagaat tcgtgatttc
    32281 gatctcaaaa tgcactttag gaaacctttt gatcagatgt ttcatcaact gaggcaaaag
    32341 atagacagca aaggtgtggg aagcaacgat ccgacatact tcttcttcat tttgttcatt
    32401 gagcaatttt tgctgagttt cttcccattc ttctaacaaa accagtgtcc gttcatataa
    32461 taagtcggct tgcttagttg gaagcatctc ttttcttccg ttacgttgaa aaagatcgat
    32521 attcaattcc cgctccaact gtttgatttg attcgagact gctggttgag ataaaaacag
    32581 tacttctgct gctctggaaa aatttcttgt ttcatatact gctcgaaaag tcgttaataa
    32641 cttaaacatg gcaccacttc cataactttt tatgataatt actataaaaa ctatttgatt
    32701 taaatataca aaaaaccatc tataatagaa atgtacaaca aaacagttgc tgatccaact
    32761 agtaacttag atgaagacaa tcagctgtct gcttgctcgt aatcatacat tcctattcat
    32821 tttacaatgt attcgttcat cttggtacta aaatctatta aataaaaagg agaatattat
    32881 atatgaagaa ctttttgaaa attattccga tcccgatctg cgggttgatc cttgggttag
    32941 cttcccttgg aaatctgttt aaagactatc accatgttgc tttaggcaat ctaactggcg
    33001 gtatctctat gattcttatg atattcattt ttgttaaatt gtttatctta ttcgaacaca
    33061 cgaaacaaac gttgcatgat ccaatcatcg cctccgtttc accaactttt acgatgtcac
    33121 tcatggttat ttgtacttat tttgtttcat atgaaagtat tgcaccaatt gtcaaattca
    33181 tttggctgtg cgctgttatc ttccaagtga tccttgtctt ttatttcaat tatcaccatg
    33241 ttatcaaagc tgatctttca attgaagcaa tttatccaag ctggtttatc atttacgttg
    33301 gatttggggt catcactgta actgccggga acttcttccc acttattgga aaaattttct
    33361 tttggatttc attgatctgc tatgcgttct tattgccgat catcatcaaa cgtgtctttt
    33421 tcgtaagaaa tatggctcat ccgacacgtc cgctgattac gatcgttgca gcacctggtt
    33481 ctctttgcct aacaggttat cttaaaaact ttgctgaacc taatttttat cttgtcgtag
    33541 cattgttcgc tttatctcaa gtattgtatt tcgttgtgct gtctatgctt ccacgtttgt
    33601 tcaagcttcg tttttatcca agctatgcag cctttacatt cccgcttgtt atctctgcaa
    33661 cagctatatt tactactgtt cattacttca acagtttagg tatctatagt agcgtattgg
    33721 atttcttaaa agttgcggaa tgtctgtttg caagtgtagt tgtcttctac gttcttgctc
    33781 attacgtacg tttcttaatt aaagaacata gcaaacatgg aaaatttgtt tctaaaaaaa
    33841 tcgcttaata atcgattcaa taggttgtga aaaagctgtt ctgcatcaag taataagaac
    33901 ggccaaacaa aaacagctgt tcatgttttt gtactggttg ggcttattac cgcagatgca
    33961 agcttttgaa caccgtttac caagaggtcg taaaagaagt ggtcagcttc aagaaataag
    34021 aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat ttccgtagaa
    34081 gctacttctg gaacaccgtt tattcgaaac aagaggtcgt acaagacttt tgtcacgacc
    34141 tcttgttctt ttgtcaaaat actggtttag tgctactatg ttcgagtatg cttttagtat
    34201 ttattgacga aaggaaccga tcaaatgaaa tcattgtcac atccaataaa atctgtaaag
    34261 gattcagcgg tccgcgaaat cttacccggt ttactgcttt cttttgccat cgctgccttc
    34321 agcaggcttc ttgctattta tctgccacag ttaggtggag cgactattgc tattttattg
    34381 ggtattttat taggaaatac tttttttaaa cagcctgttc tagcaatcgg cacaaaattt
    34441 gctgaaagca aactattaga attctctgtt tttttactag gtgcaactgt cacagctcaa
    34501 acgattgctc aaattggtgt aaaaggaatt gtttttgtgt tactgcaaat gtcagtcact
    34561 atcatcggtg cttacttgat tggtaaaaaa ctgcaattca gcgatacttc ttctttgatg
    34621 atggctggag gaaacgctgt ttgtggttct tctgctattg cttcaatcgc cccttccatc
    34681 catgcgaaag aggaagaaaa aggacagatc atcacactag tcaatctact gggtactgtg
    34741 atgatgttga ctctcccttt tcttggatta cagttgtttg gtggaaatct cattgaaaaa
    34801 agtgcacttt tgggtggtac actccaatct gttggtcaag tagtcgctgg ggcaagctta
    34861 cttgacaatt ctgttgtcga attcgccatg ctatttaaga ttctacgtat tatgatgctc
    34921 gttgttgttg tctatctgtt tgaaaaattc atgcacaaga aacaaccagc tctgtttgaa
    34981 acgccaactg atacgcagaa aaaacgaaaa ttaccgcttc cttggtatgt attagggttt
    35041 gtgatctttt gtttggtcaa cagcttgttt tctttacctg tagttattga ccaatcggca
    35101 cattttatca gcagctggtt tgagatcact gctttagcgg caattggtct gcgtttagac
    35161 tttgcaaaat tcatgaaaga aggtacacgc ttcttatctt atggattagc tgtcgggctt
    35221 ctccaaacag tgatggcttt actatttatt tggctgatca aagtttagaa tgaacatgaa
    35281 aaaacagttg tgattctcgc atggaacaat gtccgaatca caactgtttt taattttgca
    35341 gatatccttc taacaacaac aattggcgtt tgatttcaac cccatctatt gcgtttccca
    35401 tatatcctgt cagttttcca ttttgcccga tcactcgatg acaaggaata acgattggca
    35461 atggattggt tcgatttgcc tgcccaatcg ctcgcacagc tttcggacta tcaacagcaa
    35521 cggctacttc tttatacgtt cggatttctc cataaggaat ggtcgacaaa gctttccaga
    35581 cattttgctg aaaaggcgta cctctcttta tagataaagg aacatcaaat tcttttcttt
    35641 ttcccgcaaa atattcttca aactgttctt gtgtgcgcgt taaaattcgc tgattcgctg
    35701 attcgctttc tgaaattggc tgaaaagata cagcagtcaa tccggattca tctgcatcta
    35761 accataaaaa gccgataggt gtgtgtattt tcattttgtt tgctcctagt caatagactg
    35821 gaacttcctc ctttttctct atccaaaact acttgccaac taataaaaat tgttcttaaa
    35881 agtttgataa accgaggaaa catggtaaac taaatacgtt agtttacaaa aagaatacct
    35941 gttcttttac gtaaaatcaa attttaaagg tgttatttaa ggtttattaa attttgaatg
    36001 taaaccatat ataaacatac gattgaaagg atagaaacga tgaaagtatc aattgttgtc
    36061 ccatgtttta atgaagaaaa aactgttcct cttttttttg atgaggttga aaaattcaaa
    36121 ggtgcccatg agttcgaata tttgtttatt gacgacggat caaaagacgg tacgttagca
    36181 gcaatcaaga agctagcctc agcttatcct tatgtccgct ttgtctcatt ttccagaaat
    36241 tttgggaaag aagcggctct gtacgctgga ttgcaagctt ctacaggaga tttagtgacc
    36301 gtgatggatg ttgatctgca agatccgccc gaactgcttc ctgagatgat caaccggatc
    36361 actacttctg atatcgattg tgtcggcacc cggcgttcga ctcgtgaagg cgagcctgcg
    36421 atacgcagtt tctttgcgaa aaaattctac cagttgatca ataaaatcag cgatacagaa
    36481 atggtcgatg gcgctcgtga tttccgcttg atgactcgtc aaatggtgga tgccgtccta
    36541 gaattaacag aatacaaccg attttcaaaa ggattattta gctgggtcgg atttaaaaca
    36601 gaatatattt cttatgaaaa ccgcgaacgt gtggctggcg aaacatcttg gtccttctgg
    36661 aaattattca actactcgat tgatggtatc gtgaacttct cggatgcacc gttaacgatt
    36721 gcttcattta tcggcgcttt gtcttgtgtc ggttctggta ttgcaatttt gttcattatt
    36781 ttgcgggcat tgttgtttgg tgatcctaca tcaggatggc cttccatggt ctcgatcttc
    36841 ctgttcattg gtggaatcca attgttatgt cttggaatca tcgggaaata tattgggaaa
    36901 atcttcttag aaacgaaaca gcgtcctgtt tatatcataa gagaaacaga aaaagtgaag
    36961 ccaaatgaat tgaagcctca ccaataatat acgtcaaatg aagtaaacat ttttttaaat
    37021 gaaaaaggac tgtgacaaaa gtcttgtcac agtccttttt tccgaataaa tggtgttcca
    37081 gaagtagctt cttcggaaat aagccgaaat ttcacaaaaa tttgagaagc aattttcgta
    37141 aattccttct tatttcttga agctgaccac ttctatcaca acctctttca tatcttatta
    37201 cttcttcatt gctgctttta gtgctgctgc taatgggctt tcttctggtt cttctgcttg
    37261 tgcatatttt ttcagcaagc gtcgttcttc atgtttggtc attttcttct tctgctcttt
    37321 tttatcaagc aatttttctg tcgttccatc gtatttacaa cggaaataag caccgttttt
    37381 accttcgatg atttccattt tccggtgaca ttgcgggcat cgatgattag agacttttgg
    37441 atcttttctt ctgcggtagt tgcaatctga attggtgcag acatagattt tgccgtcacg
    37501 tgtgtttttc tcgcgcagat tggccccaca ttctgggcat ttcttttggg taatcgagaa
    37561 atcttgatat tcttgtttgc ttgatttgat ctcattcacc aagcgttttg tttctttttc
    37621 gattccattc aagaaggttc ctgcttgttt ctttcctgca gcaatagctt caaggtcttt
    37681 ttcccagctt tctgtcaaat ctggtgttac taaagaggga tttaccaata ctaataattg
    37741 cttaccttta ggagatactt gcagtccttg aggggtacgt tccatcagtt cagatttgat
    37801 tagtttctcg ataatctctg cacgcgtagc tggggttccc agactgtatt tttccatttg
    37861 accaagcaag cttgcttctg acaatggttt aggcggtgca gatagttctt ttttgatggc
    37921 gaaatcggga ttgattctca ttcctttttc ccatttgact gtttccgtct cagtttcagt
    37981 ttcgcctttc catcctgctt ggacgacacg attttgccga aaaatgaatc gttctttacc
    38041 aaattcaacc gtaacttttg tttggctgac tttatggggt tgagcaaaca gtcctaaaaa
    38101 acgagagaca atcatttgat agatttttcg ctcttcatta cttaattttt cataacgtgc
    38161 tctgttttca gttggcaaaa gtgcatggtg atcggtcact ttactgtctt ggaatacttt
    38221 tgtctgttgg acgatggctc cgtttttcaa atactgcttg acctcaggag caaagtcgct
    38281 gactgcttgc aatcgttctt tcatcgtact tttcatgtca tttgtcagat atttgctgtc
    38341 tgtacgcgga taagtaacga tcttatgggt ctcatacaaa ctttgtacaa gacttaatgt
    38401 tttctttgca gaatagccat agcgttgatt ggcttctctt tggatctctg ttaaatcata
    38461 aggcaatggc gcagattccg tttttaattt ttcttggact tctgtgacag ttcctttgct
    38521 tgtactgatc aattgaacaa gtttttgtgc ttcttcttgt gtttttaaag caaattgatt
    38581 tttttgaagc attttggctt gttcttttcc agcttgcaac gtgatcgtga aatacgtttg
    38641 tggacggaac tgctcgattt tcttttcttg gtctctcact aatgctagtg tcggtgtctg
    38701 cacacggcct gctgataaat tatccgcgta cttcacagtt aaggctctgg tcacattcaa
    38761 tcctaccagc cagtccgctt ttgcccgggc aagagcagat tcatacaatg catcatattc
    38821 tttcgcaggt cttagttgct tgaatccagt tttgattgct ttatctgttt gagaagagat
    38881 ccataaccgc ttcactggtt tattgaaatg cacatactct aagatccagc gagcaacaag
    38941 ttccccttct cttccggcat ctgtcgcaat gactgcttct gagacatctt ttcgctgagc
    39001 cagctgcttg attgctttta attgatgtcc tgttttagga agtggtttga ttcctagttt
    39061 tttagggatc attggcaacg tgtccatttg ccagctctgc cattccttat tcagatcttc
    39121 cggcattttc aatcctaata aatgaccaag ggcccaagta acgatcacct tgggtccttc
    39181 ataataattt ttgtgctttt gattagcacc aagcaccttg cttaggtctt tcgcaacgct
    39241 tggtttttcc gctagaatta gttgtttcat aaaattcctt tctgctttct actgtgatgg
    39301 gctgatgatt tttcaacaac gcaagtccct tgtattgctg taaatcatcg tcacactctt
    39361 cacaccagcg ctcatctccg tcattccaaa aggcgctgag aaagttactt tttgcattga
    39421 tatattcata attttgatgg atttcgttcc attgtttttc gtacgcttgt cgtgcttctt
    39481 cgaaagactc gaatgtttct tcttcttcaa tatcttcttc ccagtcttca aaaaaccacc
    39541 aaggctcatt gtccccgaac atggtaatta cttgatacat actcgtgctc cttctttcag
    39601 cttttctaga ctcatcttag tcacttttca agaaagttgc aagaattttc tcttataaaa
    39661 gggtcattta atgaacatct taaaattgtt acaaatgaat ctgattgttt aaggcaaagt
    39721 agcgataaag ttattgatca tccaaaaata gctgtcttct ggatcgattg ttggttcttt
    39781 tgtaaagtat cctaaaaaac gtaaggaaag ataaccgtgg atcatactac gaagttccct
    39841 gcttcgatga actttttctt ctttcgttaa ttgaaaagcc tcaaatatct gcaaaatgat
    39901 ctgattcgtt tcatggattc catctaataa aacagcttca ttggtatgcg gtacactgat
    39961 cagtaactca tacactgctt gatttgaaaa agcaaaggat cgataagttt ctgcatagat
    40021 ctgcagtgct tcctgaccac tttttcccac aagagcccgt cgcagctggt cattaagttc
    40081 ctgcagaagt ttcgctgtta gcgctgtttt gacatcacga atatttttga aatggttata
    40141 cagcgaggga gccttgatat ccaagcgttt ggctaaatgc tgaaaggtta tttgctgaac
    40201 atccatctct tctgctagtt ctcgaaagca gtcgatgatt ttctcttgag acagatttct
    40261 ttttttcatt tccatgtttc ctctcataaa ataaatcctt cactctttca gtttaccacg
    40321 aaaaagaaag agaaaaaaga gactttcatt gataaactac atattgtagt ttataataaa
    40381 gagctgtaga tgaaaatttt cagaaagaag gagtctaatt ggctgcaaga ataaaggaaa
    40441 aattatttag gaaaagagaa acttgggaac aaaacttgtt tgtcttatgg tttggaacat
    40501 ttatggctgg tatcggattt agtttagtca tgccttttat gtcactttac atagatacgt
    40561 taggtaatta tagtacgtca caattgaact tttggagcgg attgactttt tcatctactt
    40621 tcttggtgac tacgcttatc tctccttggt ggggaaaatt agccgaccaa aaaggaagaa
    40681 aattgatgct gttgcgtgct tcattaggaa tggcaatcgt catttctttg atgggttgcg
    40741 tgactagtgt gtatcaatta gtcgcattgc gtctgcttca agggattttt tctggttata
    40801 tcagtaatgc cactgccctt gtcgcaactg ggacgccaaa agaaaaaagt ggtcaagttc
    40861 ttggaacctt agcaactggt tctgttacag gaacattact tggaccgctt cttggcggga
    40921 tctctgcttc agcgtttggc tatcgcccta cctttttcat tacagggatc atcttatttt
    40981 tcgtcttctt gttgagtctg tttttcgtcc atgaaaaatt tgtcccagtg aaaaaagaaa
    41041 acatggtttc tgccaaacag attttcaaag acttgaaata tccacacgtc gtcatcggta
    41101 tgttcgtcac aacgatgatc atccaagcat ccaacaattc cattagtcca atcatcagtc
    41161 tgtacattcg ccaattgctg catggacatg ggaatgttac gttgatcagc ggaatcattg
    41221 cttcgatacc tgggattgcc actttgatag ccgctccaag atttggtcgc ttaggtgaca
    41281 agattgggag tgaaagaata cttgcgattg gtttgggttt tgcgattctg gtttatatcc
    41341 caatggcatt cgtcacgaat gtttggcaat tagctgcctt acgttttttg attggtatct
    41401 ctgatgcttg tctgctccct gctgtccaag cattgatcac aaaatattcg cctcatcaag
    41461 ctgctggacg gatcttcagt tataaccaat ctttccaagc aactggtaac gtagtagggc
    41521 ctatgatcgg ctccagtgtt tccagtgcat ttggttatcg tggcgtcttt ctttcgactt
    41581 ctcttcttgt tttggtcaac ttcttgcttg tacgacacaa tacacaagag atccatcagc
    41641 aagaaactgc agaagaaaaa gcttcaggag tctccatagc ttcagggcac tctccttctc
    41701 atagacaata agcgtaaatg aaaaacactg cttgaacagt tgtcacagac tgtccttgca
    41761 gtgtttttgt ttaggcctgt tttttcaaga actcagataa gaggaataat cccttttcca
    41821 aggtttcttt tttacaacag taacctagtc gggcatgctt cggtaaatca aaggcacttc
    41881 ctggtactaa tagtactcct gtttcttcta ataaattgat acaaaatgtc tggtcatctt
    41941 cttcgatggc taatttaatg aaagaagtag agacgtaatt gggagcgaca agttcaactt
    42001 tcggttcatt ggctacccat tgttttaaaa tcacaaggtt ctctgtaatg atctttttgt
    42061 ttcgcgccaa tattttttct ttattcttca atgcatgtac agcaagatca tctgacagga
    42121 ctccgccgca aatcatcgtg tagtctcgat actttcgaaa aacttctgcc aactcaggtc
    42181 cagtagctgt ccagccaata cgaatacctg gaatggagta ggttttggat agggaattgg
    42241 ttgctatacc tttttcatag acatctacga tcgacaagaa ttcaccttta tccgttaaag
    42301 gcgcatatac ttcatctatc aatacatatg catctactgt acgagcaatt tctgcgattt
    42361 tttctaaagt tttccgatct agcaatgtgc ctgtaggatt gtttgcacta ttcaaacaga
    42421 tgatttttgt ttcaggtttg actaaggctt ctagctgttc taaatcaaac tgccaatctt
    42481 cttcttcctt caaatgaaca aagtcaacag cggcacctaa agatttgggg atgtcatata
    42541 attgctgata agtaggaagc atcgaaacaa catggtctcc tgcttccact aacgcgtata
    42601 aagctaaaag attagcacca gtcgctccat tcgtttgtaa gatgttttcc ggatcgacgg
    42661 tttgataaag agaagcgact tcttgtttga aaacatctga tccttcgatc cagccatagt
    42721 ccaatggttc gtttttgcgt ttttcgaaaa atttacttac gctagtccca tctaagccaa
    42781 cgagttcgtc taatgtcaat gcttcgatcg agctttgtgc aatatcatat accgctttct
    42841 tttcccattt atttagccat tcttctacac cgaaatttgc gattttcatg tttctttccc
    42901 ccttatatct tgatccagcc tacagcaagc aacccgattg aaagcagtgc ggccacacaa
    42961 atcagcccaa tcattacttt ttcttttccc gtaatttgat gtttggtttc acgacaagcg
    43021 atccagtaga agcaaaaacc aggaagatag gcaatactac ataaaaggac ataagaaaat
    43081 cctgctaaaa tgatgcccat caattcaaaa actacagcaa tcccaccgat cactacttgt
    43141 tttttgattc cttcttttaa agaaaactgg acttgataga tcccgacaag tagataacaa
    43201 atcaaaattg ccgctgtaca taaagaatag gcaaattgat aggcataatc agtaactaag
    43261 aaagtcaata agaatagatt agtcaagatt gcagtaacaa ctaacgaata agttggggca
    43321 ttttttttat tcgtttttcc ccaacctttc ggcaacagac cgtctcgagc catcaacatc
    43381 gttgtttctg ctgggagcat cgtccaagac aaccaagaac caatgatcga aataatcaag
    43441 ccgatattga tcagcgctgc tccccaattt cccacgattc cttttaaaat atttgccata
    43501 gcaggttgtg aaatagttgc taactcttct tgagataaga cgccgtatgg caagatcgaa
    43561 gcaaggatat agataaacaa taaacaaagc agaccaatga tcgatgcttg ctgggcttct
    43621 gaacgtttct tcgcgcggtt ggctaaaaca cttgcgcctt cgatccctac aaagacccac
    43681 atcataacca tcaaacaacc tttaatctgc aaccagatcg ttccttgctg ccctgttcca
    43741 ttcactagat tatcagatac gttaccccaa aaatcagcag tgaatatatt cactttaaat
    43801 gcgataaata caaatattaa aaataggaat aatggcacta atttacagat cgtcacgatt
    43861 gtattgatga cgctcgctcc ttcaactcca ttgtttacta ataaagtcag accccaaata
    43921 aaaatacttg caaataaaat agaaggaaca ttttgtccac ctttgaatat cgggaaaaat
    43981 tctcccatgg cgctcatcag catcgttgca aaagcaacat ttcccagcca cgcagacaac
    44041 cagtaacccc aaccactgat gaagccacct aatttaccaa atgaagcttc cgcgtaaccg
    44101 aaaataccac catctagatc tggacgcttt tctcctaaat gattcagcga taataccaaa
    44161 gctaaaatac caatcccaac gattatccat gaaaggatcg ctggccctgg agctgcagaa
    44221 ctagccagat cacttgtgat cccaaacaca ccgcttccga ttgacgaact gacaaccaga
    44281 gcaaccagtc cccatttgct aatacccgtc gatttctttt ccatttccat caccctcact
    44341 tataaaattg aacaaatcac ttgcaaaaga ttcccttcta ttttgcaact gctcttgctt
    44401 aacaaaaagc aactttattc atattattgg atattatgaa ctttttacac aagtatttca
    44461 agtataaaat gaataaaaat taattttatt gcaaaaaaac agctatgcta tccaaaagaa
    44521 aaggtgtttc ttttgttagc catagctgtt ttgattcatt taatatcgct taaacgtatt
    44581 gtaaaacttt tgtttcttga tatgcgtggt cgatcaaacc accacccaga cattccattc
    44641 catcataaaa tacaactgct tgtccaggag tgattgcacg tacaggttcg tcgaatacga
    44701 cttctgcacg attatcatcc aataagcgaa ctgtcacagg aacgtcttgc tgacgataac
    44761 ggaatttcgc tgtacacttg aattcttttg gcattggttc attcgtcgta aagtggattt
    44821 cacttgcatc caagcttgtg gcatacaatg caggatgatg gaacccttgc cccacatata
    44881 atgtgtttgt cgctagatct tttcctacaa caaaccaggg ttcttgcgaa tctcctccgc
    44941 caccgatccc tagtccttga cgttgaccaa tcgtataata tatcaagcca gcatgttgtc
    45001 ctttgacttc accatcaaga gtgaccatat ttcctttttt tgctggcaga tagttgctca
    45061 aaaattgttt gaagttcttt tcaccaataa agcagattcc cgttgaatct tttttcttag
    45121 cagtcgcaag gcctgcgcgt tcagcaattg cccgtacttc tgatttttcc atgccgccta
    45181 gagggaacat cgtttttgaa agctgttctt gtgataattg gctaaggaag taagtttgat
    45241 ctttattatt gtctactcca cgaagcatgt ggaccacgcc gttttcatcg cgagtgactt
    45301 gcgcataatg tccagtagct acataatcgg cgccaagatc cattgcataa tccaagaatg
    45361 ctttgaattt gatttctttg ttgcacatca cgtctgggtt aggtgtacgt cctgcgcggt
    45421 attcggctag gaaatattca aatacacgat cccaatattc tttttcaaaa ttcacagagt
    45481 aataaggaat cccgatttga gcagctactt tcgctacatc cttataatct tccgtggccg
    45541 tgcatacacc gttttcgtcc gtgtcatccc aatttttcat gaatattccg atcacgtcgt
    45601 atccttgttc ctttaacaaa agtgctgtta cagatgagtc gacaccgccg ctcatcccta
    45661 cgacaacgcg tgttttgctg ttgtctgtca tgatatcacc atcatttcaa ttagattctg
    45721 aaacatctac gagttgaagg tcgcaacttt tcagtatttt acattatacg tcatatttct
    45781 gaaaatttca tcccttaatt atcaagtcgt aaaaatactg ttctactgga cctattcagt
    45841 acacaaacat gatagtcaac caaaaaatca ctcatctttt gagcattcga tataaaaaat
    45901 tggcatactc ataaaaaggg ccagcctttc ggcaataagg aataatttta taaattgtga
    45961 aaaactattt ttcaaaattg cttcttatcg cttaaagctg cacgtctcat ttacaacctt
    46021 ttctttttag atgttatgag ttgctcagat aagtgatttt gtttactcta agaaaaaagg
    46081 gaggcaaaag atacatgaat tactttatca gtgatatgca ctttttccat gaacgcttat
    46141 taggaaaaaa cgactttgct ccgcggctct ttgcaaatgt ggatgaaatg aaccaacagt
    46201 tgatcactag ctggaatgca gtaataaaag aaaccgacca tgtttatcat ctaggcgacc
    46261 ttgctatgca tccgcaatat gaaaaagcaa atgcagaaat tttggaactg gtcactcaac
    46321 tgcaaggaac aatccacttg atcaaaggga accatgacag tcgcgctttt ttccgttatt
    46381 tagaaaaaaa tgatcctagg cttactgacg gaagtcccaa attttatttt tatgatgtag
    46441 gtgccatcgt taaattcgac catcaacaat attacttgac tcattatccg atgttgctcg
    46501 ggcctaacga cactatccgc aatcttcatg gacatatcca tcattacagc gtacctatcg
    46561 agaacgatgt caatgtaggc gtagacgcac cggaaagaga actcttaaag gaaaaactac
    46621 catttggcac tccgttatct gagcaacaaa tcaaagaaat ctatgaagca aagaggaagt
    46681 acgataggtc gtgaaaaaac tgccctatat caagtattaa acaaataaag ataattactc
    46741 atatttttca tttgagtagg cttattgcca caggaacatt aggatcagaa atggagttgt
    46801 aacaagactt ctgtcacaac tccattccat actattcgtc tttttgctca tggtgttctt
    46861 ttaccagttt tttctgtaat tctttatcat aatttcgggc agctgacgtt aaaaataaaa
    46921 cgattccgca taataaaatc ccgattccgg aaaacaagaa cattttttct acccctaaac
    46981 gatcagctaa tggtcctgca aataataatc caattggacc tgccaggttc atcaaagagt
    47041 tgaagttccc taagacacga cctaaaacat tcgattcata actctgctgg atcattgcca
    47101 tcagcagtgt attgaaatat ggcgttgcac aacctgctcc tgcattcaag ataagaaagt
    47161 acaagaaccc ttgactatcg tttggaacca atccgcttgc accgatcgtc aggcccataa
    47221 tgatgtaggc tataataata ggtttcattc ggtctttcca tgttcctata aaactgatca
    47281 gtgcaccgcc taagagcatt ccgactgcgt agactacttc cactgctccc gcctgtccaa
    47341 cggaaccacc aaagtaatct agcgtcatca atggatacag actgattgct ggcatgaaca
    47401 acagcatcac aaatgcacca ttgatcgtta tgtaccataa ccctttatta tccatcaatt
    47461 gctgcagccc aaattttgca tctttaaaaa tagtaagcgg aacttccaat atttcaggag
    47521 aaacttttgg gatcttcaca aaaatcaata agccgacacc aaaaactgcc cccaatacat
    47581 ctaataaaat aaggtaattg acaggaacga ctgaaaacaa agccgcacct agagctggcg
    47641 caatgatgaa attcgctgat tggaccattc ctaactgtcc attcgtcctt gttatgtgag
    47701 aagatggaac gattgtcggc ataatcgact ggatcgttgg catttggaat gtttgtgcga
    47761 ccgaacgcat aaacaaagac acaaaaacta gctaaatcgg gaaggattct gaaatcgttc
    47821 cgactactgc taaaataagc gcgaaaatcg ctacaataat atccgtgaca atcaaaagag
    47881 cttttttatc ccagcgatcc actagcggtc ctacaaatgg gcttagtaaa accatgggaa
    47941 tcattcctag taaagtagca aaactcaaaa ttgtcgccga accggtttct cttgtcaaat
    48001 accagatgat cgcatattgg acgaccatac tagtgattcc tgacaaaaac tgtcccgata
    48061 aaaagaacaa ataatttctc ttccaattcg ttaaaacgac cgactgttca ttttccataa
    48121 acatccctct tttctttttc attttgtata gtgtttgcac aacaatcatt atacgggatt
    48181 cttttttcaa aaaatacctt tttccctaaa taacaatcgt ttcaatttaa atttctttac
    48241 ttagaaacaa taaatcagca aaatattaaa aataacaaca tgtgttaaat ttaaaaataa
    48301 aaaagacaac taatttgcgt cgtccttaca aagttagtca tctaacaata ggccgtttca
    48361 aaactagttg tctttttata tccacagatc attcgacttt ttcgaataac ccacggctaa
    48421 tcatcccatc catcagaaaa taaaatttat cttggctcat tttctgtttt ggatctttga
    48481 aaatattgac tgctttcaat ccatttgctg tagtgatcga cattttcaat gtttgaatat
    48541 cttttgatac agtgattttt aatttgaatc cttctttgct ttgcggcgtg gcatctaatg
    48601 gtcggatcaa ttgataattg cctccgtttg tttctgtaaa tccattatcg cgcaatgtat
    48661 atctttttgc atcaggatgc aaacgatagc taattggtgc gcccagtacc gttgctgttg
    48721 tttcaaatgc catgattttc acctcgataa aattagtttc tctttattat aaaagtttat
    48781 cctgattttt actaggtttt tcagttaagt tccctaaaaa atgaaggcgt tctctcagac
    48841 atctgttcat ataagaatga cttcactagt tattttttga cagcacgaac taattgatca
    48901 aggaaatact ggatgtcgtc ttcggtattt cccaaaccaa agctgatacg gatggattct
    48961 tttagtatcg gtgtatcttc tccatacatt gcttctaata cgtgtgaagg ctcgatattt
    49021 cctgctgtac aagctgaacc agtagaaata gcgaccccat taagatcaag cttcatcagt
    49081 agcaaatcat ttgcttcgcc aattaagcga agattcaaca catgagggag tttattcgtt
    49141 aaatcaccat ttacttgata agggatttcg tttgcttcta atgtatcaag aatcaattgc
    49201 tggaatcctt tatatttgtt gttacgtgct tcacgttctt caggtgacag cagttcaact
    49261 gcttttgcca taccccagat ccctgctaaa ttttctgtac ctgcacgccg cttttcttcc
    49321 tgctccccgc ctaaaagcaa tgcagggata tttactccgt ctcgtttata taagaaaccg
    49381 acgccttttg gcccattgat tttatgagca gagatactta ataaatcgat tcctaattct
    49441 tctggatgga tcgtttggtt tccgtacgct tgtaccgcat ctgtatggaa ataagctgga
    49501 tgatccgcta aaagttcacc gatttctttg attggcatca aatttccgat ttcattgttc
    49561 ccgtacatca cagagaccaa gatcgtatct tctcgtaacg cttttttgac ctcttcgatc
    49621 gacagattcc ctttttcatc tacaggcaga tatgtaacgt caaacccctt tgtctctaaa
    49681 tatttcatgg tattaagaac agcaggatgt tcgatagctg tagtgattag atgtctgcct
    49741 tctttttgac gcgagaacgc tgtttctaag atagctgtat tatccccttc tgttccccca
    49801 ctgttaaaga tgatctcgtg aggttttgct tgcagactgt ctgcaatgat ttgtcttgct
    49861 gtttccaatt tttcatgcgc gtggcgacca aatccatgta tgcttgaagg atttccaaac
    49921 gtgtcttgca tgattgccgt catcgcttca attacttgtg gatgcatggg ggtagttgcc
    49981 gcatgatcca gataaatagt tttcacttca ttcatccctt ccaaattatt gctaggcaca
    50041 ttctagcata aaatagcgaa caaaccaaag aagagctcat ttttgtctag aggacgtttc
    50101 atgggcatag aaaaaaggag ctgtgacaaa agtcttgtcg cagctccttg aagctgctca
    50161 cttctgtcac aacttctttc cctctttttt ttgtttattc gaattctttt tcttccagac
    50221 ggaataacgg actcatcggt gtattttcat ggatgcgttt gaccgcttca cccattaatt
    50281 ctgaacatgt aacgattgtt aagttttctg gatgacgatt ttcttctgtg aaaacagaat
    50341 cagtgataca aatatctttg attggtgcat tgtctaaaat acttttcgca tgatcggaca
    50401 ataatccatg tgacgcacag gcatatactt cttttgcgcc gttttctttt aatacatttg
    50461 ctgctcgtgc aagcgttgct cctgtgttca agatatcatc taccatgata catgtctttc
    50521 cttgaacatc accgatcaca taaccaccct cagttgaagc atcatctgca tgatctacga
    50581 tggctaatgt gctgttcaaa tattctgaaa gactacgtgc acgctgaaca ccgctgtttt
    50641 ttggcgaaac aactacgatg tcatctcccg tcagacctaa ttgacggtaa taatgagcaa
    50701 acaacggcat cgtaaataaa ttatctacag gaatatcaaa gaatccttgt acttgaaccg
    50761 tgtgcagatc taaagtcagt actcgagttg cccctgcttc ttcgatcaag ttagcgatca
    50821 atttcgcagt gatcggttca tgaggtttcg ccgtgcggtc ttgcctagca tacccatagt
    50881 aaggcaatac gacattgatc gttttcgcac ttgctcgctt cattgcatcg atcatgatca
    50941 gtagttccat atagtaatca ttgacaggtg cattcgttga ttgaacgata tacacatgat
    51001 cccctcgaat actttcttcg atattgatgg aaatctcgcc atcactaaat tgcttcacga
    51061 cgcttttacc taattctgtt ccgaacactt tcgcgatttt ttcagctaat ggacggttgc
    51121 cattcaaact aaaaatcttc aacgtgtcat cctgatactt attactcatg tagtcctccc
    51181 caaaacttct ctattcttcg atagtttaac atatttttcc aagattttct gttaatttca
    51241 gcaaattctt aaggaagtga aactgttccg ttactccaac tggcaggaat gactttatga
    51301 taatcgcccc agttgtcgtg tactttgtaa taacgttctc ctgtttcagt ttcaaaataa
    51361 gcgtacgctg tcacccaatg attgccatat gtactgccaa gtactttcaa aataccaatc
    51421 atcacaggtt tgccttcacg tatacgtttt gtagctcgct gccatgaacc tagaagagtt
    51481 gctcgtgcac gctcatgatt gccattcttc tttaaaaaac tagaaatccc tgtactaacc
    51541 tgaaaaggaa tcgtaggaag tcctagtggt tggatctggc tacgcaatgc cttcgtcaaa
    51601 accaacgaat cagccgtatt tttcttgcga atctcatttg gaagcatcca ccccttacga
    51661 tagtcttgat aataggcaag gagaactgct gccgcgtaag taccacaaag atagcctgag
    51721 ggggtagccc atttcttata cctcaaaaaa cgttcttctg gcaatcccac ccagcaattt
    51781 gcgagttcct ttcctgacct tttttcttcg atcaaaacgg gaatatcttc ttttctgttg
    51841 atccatctag ataactcgat tggctcttca ttgatcagct tccatttttt acaagaaaga
    51901 ccctcttgat aagtatattt tgctggagaa tattctaccg gatgtcctga ttgatcgata
    51961 atcacataac tttgatcttc aaaaaccaga gctaaaaaaa gcaatttact ttttcttgac
    52021 tgcagctgct gagtgctggc taatttttta atttttttat cta
//
LOCUS       DACDRN010000011        49594 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDRN010000011 DACDRN010000000
VERSION     DACDRN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.011"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(26..1957)
                     /locus_tag="IWJ80_000878"
     CDS             complement(26..1957)
                     /locus_tag="IWJ80_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9460635.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(1975..2889)
                     /gene="pfkB"
                     /locus_tag="IWJ80_000879"
     CDS             complement(1975..2889)
                     /gene="pfkB"
                     /locus_tag="IWJ80_000879"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9460636.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2886..3638)
                     /locus_tag="IWJ80_000880"
     CDS             complement(2886..3638)
                     /locus_tag="IWJ80_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9460637.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3797..4006)
                     /locus_tag="IWJ80_000881"
     CDS             complement(3797..4006)
                     /locus_tag="IWJ80_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460638.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4017..5345)
                     /locus_tag="IWJ80_000882"
     CDS             complement(4017..5345)
                     /locus_tag="IWJ80_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460639.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(5710..6297)
                     /locus_tag="IWJ80_000883"
     CDS             complement(5710..6297)
                     /locus_tag="IWJ80_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP9460640.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6380..7630)
                     /gene="clpX"
                     /locus_tag="IWJ80_000884"
     CDS             complement(6380..7630)
                     /gene="clpX"
                     /locus_tag="IWJ80_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP9460641.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7877..9166)
                     /locus_tag="IWJ80_000885"
     CDS             complement(7877..9166)
                     /locus_tag="IWJ80_000885"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP9460642.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9320..10252)
                     /locus_tag="IWJ80_000886"
     CDS             complement(9320..10252)
                     /locus_tag="IWJ80_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460643.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10409..11068)
                     /locus_tag="IWJ80_000887"
     CDS             complement(10409..11068)
                     /locus_tag="IWJ80_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9460644.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11071..12096)
                     /gene="sppA"
                     /locus_tag="IWJ80_000888"
     CDS             complement(11071..12096)
                     /gene="sppA"
                     /locus_tag="IWJ80_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9460645.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGASGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12109..12399)
                     /locus_tag="IWJ80_000889"
     CDS             complement(12109..12399)
                     /locus_tag="IWJ80_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460646.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12809..14614
                     /gene="glmS"
                     /locus_tag="IWJ80_000890"
     CDS             12809..14614
                     /gene="glmS"
                     /locus_tag="IWJ80_000890"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP9460647.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14686..16041)
                     /locus_tag="IWJ80_000891"
     CDS             complement(14686..16041)
                     /locus_tag="IWJ80_000891"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP9460648.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16065..17234)
                     /locus_tag="IWJ80_000892"
     CDS             complement(16065..17234)
                     /locus_tag="IWJ80_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460649.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17231..18118)
                     /locus_tag="IWJ80_000893"
     CDS             complement(17231..18118)
                     /locus_tag="IWJ80_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP9460650.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18307..21987)
                     /gene="nifJ"
                     /locus_tag="IWJ80_000894"
     CDS             complement(18307..21987)
                     /gene="nifJ"
                     /locus_tag="IWJ80_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP9460651.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            22185..22706
                     /locus_tag="IWJ80_000895"
     CDS             22185..22706
                     /locus_tag="IWJ80_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9460652.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(23405..23970)
                     /locus_tag="IWJ80_000896"
                     /pseudo
     CDS             complement(23405..23970)
                     /locus_tag="IWJ80_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24149..24805)
                     /locus_tag="IWJ80_000897"
     CDS             complement(24149..24805)
                     /locus_tag="IWJ80_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460653.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(25373..26308)
                     /gene="whiA"
                     /locus_tag="IWJ80_000898"
     CDS             complement(25373..26308)
                     /gene="whiA"
                     /locus_tag="IWJ80_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP9460654.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(26342..27340)
                     /locus_tag="IWJ80_000899"
     CDS             complement(26342..27340)
                     /locus_tag="IWJ80_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP9460655.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(27337..28221)
                     /gene="rapZ"
                     /locus_tag="IWJ80_000900"
     CDS             complement(27337..28221)
                     /gene="rapZ"
                     /locus_tag="IWJ80_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP9460656.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(28356..29087)
                     /locus_tag="IWJ80_000901"
     CDS             complement(28356..29087)
                     /locus_tag="IWJ80_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP9460657.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(29091..30356)
                     /locus_tag="IWJ80_000902"
     CDS             complement(29091..30356)
                     /locus_tag="IWJ80_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP9460658.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(30619..33435)
                     /gene="uvrA"
                     /locus_tag="IWJ80_000903"
     CDS             complement(30619..33435)
                     /gene="uvrA"
                     /locus_tag="IWJ80_000903"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP9460659.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(33445..35439)
                     /gene="uvrB"
                     /locus_tag="IWJ80_000904"
     CDS             complement(33445..35439)
                     /gene="uvrB"
                     /locus_tag="IWJ80_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP9460660.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            35698..37200
                     /locus_tag="IWJ80_000905"
     CDS             35698..37200
                     /locus_tag="IWJ80_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9460661.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            37202..37939
                     /locus_tag="IWJ80_000906"
     CDS             37202..37939
                     /locus_tag="IWJ80_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9460662.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(38307..39473)
                     /gene="dnaJ"
                     /locus_tag="IWJ80_000907"
     CDS             complement(38307..39473)
                     /gene="dnaJ"
                     /locus_tag="IWJ80_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP9460663.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(39624..41453)
                     /gene="dnaK"
                     /locus_tag="IWJ80_000908"
     CDS             complement(39624..41453)
                     /gene="dnaK"
                     /locus_tag="IWJ80_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP9460664.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(41504..42067)
                     /gene="grpE"
                     /locus_tag="IWJ80_000909"
     CDS             complement(41504..42067)
                     /gene="grpE"
                     /locus_tag="IWJ80_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP9460665.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(42161..43204)
                     /gene="hrcA"
                     /locus_tag="IWJ80_000910"
     CDS             complement(42161..43204)
                     /gene="hrcA"
                     /locus_tag="IWJ80_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP9460666.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(43439..44605)
                     /locus_tag="IWJ80_000911"
     CDS             complement(43439..44605)
                     /locus_tag="IWJ80_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP9460667.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGNRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(44621..44848)
                     /locus_tag="IWJ80_000912"
     CDS             complement(44621..44848)
                     /locus_tag="IWJ80_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP9460668.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(45235..45491)
                     /locus_tag="IWJ80_000913"
                     /pseudo
     CDS             complement(45235..45491)
                     /locus_tag="IWJ80_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            complement(45834..46857)
                     /locus_tag="IWJ80_000914"
                     /pseudo
     CDS             complement(45834..46857)
                     /locus_tag="IWJ80_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(47495..48169)
                     /locus_tag="IWJ80_000915"
                     /pseudo
     CDS             complement(47495..48169)
                     /locus_tag="IWJ80_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            48692..49282
                     /locus_tag="IWJ80_000916"
     CDS             48692..49282
                     /locus_tag="IWJ80_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP9460669.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
BASE COUNT        14053 a        10630 c         8450 g        16461 t
ORIGIN      
        1 aaaaccagcc tcttcttttt tatttctatg cttctgctgc tgcaggagct tttctcaata
       61 aaccataaat cacaccagat acgactgctc cgatagcaat aaacaccaaa tacatgaccg
      121 ggtgactcag taagaataca acgaaaatcc cgccatgagg agccagtaat ttgatcccga
      181 aagctccgac taatccgcca gttaaagcag aaccaataat aaaacttgga atggcacgaa
      241 tcgggtcagc tgctgcaaat ggaatcgatc cttccgtgac aaacgatagt cccatcacga
      301 tgttcgtcaa accagcatct tgatctgttt tgctgaattt attcttaaac aaacgtgtag
      361 caacgaaaat tgccaatgga ggtaccattc ctccagccat aacagaagcc atcacgacac
      421 ttccgccagt tgcaacagtc gtagctaaag ttgctgtacc aaacacataa gcggctttgt
      481 tgattggacc gcctaaatct gccgccatca ttccagctaa taatgcacca agtaaagcag
      541 cattcgaacc actcatactt gtcaagaaac cgttcaatgc atcattgatt gctttcatag
      601 ggacattgac cagcaacatc aagaaaccag taatcagtaa gccaaacact ggataaaaga
      661 ggatcgtttt aattccatcc aatgattttg gtaagttctt gaataattta cgtaaaaata
      721 cgatgacata accagctaag aatccaccaa tcagtgcacc taagaatcct gcaccgcctg
      781 tattagctaa tgcaccagca gcaaatccaa cgatcaaacc agggcggtca ccgatacttg
      841 aagcaataaa tcctgctaat accggcagca tgaaaccaaa tgcagcttgt ccgatttggt
      901 tgaaccagct agcagcttga ttgtaattcc ctagatttga taattgatca tgtggcacgc
      961 caataaattg gtcgatcatg aaagaaagcg caatagcgat tcctccacca ataacgaaag
     1021 gtaacatgtg tgatacgccg ttcatcaaat ctttgtaaat tctttgtccg atagtaccat
     1081 ccgcatttcc ttcttctgaa ctgctttctt ttccatcacc atggaatact ggtgcagtac
     1141 cactaagggc tagattgatc agctcttctg gtttacggat accatcactg acaggacgat
     1201 tgatcagttc ttttccgtca aagcgattca tctctacttt tttgtctgct gcaacgatta
     1261 caccttctgc acgggcgatg tcttcagcag tcaatcggtt tttgattcct tctgaaccgt
     1321 tcgtttcgac tttgatctcc acacccattt ctttcgcttt tttcttcaga gcatcttctg
     1381 ccatgtaagt gtgagcgatc cctgttggac aagctgtaac ggcaacaacg aattttcttt
     1441 ctggatgagc agaagcttct gcagcttgtg tttcttgttc tgctttttct tctgcttctt
     1501 tttgctcttc agctgtttgg aacaagtctt ggacttcttc tggtgtattt gcttctttta
     1561 attttcctac gaaatctgca tcaatcagca atcgagatag tgctgctaat gcttgcaaat
     1621 gtgtgtcgtt tgcgccttct ggagctgcga tcatgaaaaa cagataagtt ggctgtccgt
     1681 ctaaggcttc ataatctact cctttttggc tttttgcgaa taaaactgtt gcttctttaa
     1741 ctgcactgtt tttcgcgtga ggcatggcga ttccatcacc aagacctgta gaagtttgtg
     1801 cttcgcgggc tagaattcct tctttatatg tttcgatatc ggaaatccgg ccggcatcaa
     1861 acattttttg gaccatttca tcgattgcgc cttttttatc agtcgcttgc aaatccatga
     1921 tcatcgcttc tttgaccatt aaatccttga tattcatgat tatcctcctt gaaactaatc
     1981 aatttttgtg atttcaactt ctggcagcaa ctcatcaatc aattcgcgag ctgccaagtc
     2041 atcagaaaaa gctgttgcgc ttccgcaagc aactccccat ttaaaggctt cgattggatc
     2101 tttcgtacgg ctgtaagccc caataaagcc ggcaatcatt gaatcccctg cacctactga
     2161 atttttcaat gggcgaacta atacattcgt tttataagtt ccttgttccg tgaagagcaa
     2221 agcaccgtct ccagccattg agatcagcac attctgtgcc ccttcagcta gcaatttttt
     2281 accatacgga atgatttctt ctacagattg gaaggttgta ttgtaaagct cagctaattc
     2341 gtgattgttt ggtttgatca acaatggttt cttaacaagt gcatttttta ggtcatcacc
     2401 tgttgtatca atcacgaaat ctgcaccttt ttctcgaatg atatcaatca gttcttgata
     2461 aaatcctttt cgcaggctag ccggcgcgct tcctgacaat acgacgatat ctccttgtcc
     2521 tactgttcct aatgcttgct tcaactcatc gatttcttca tctgaaatag atgggcccaa
     2581 accgttaatt tcagtttcag tatctgattt tagtttgata ttgattcttg tatcttcttt
     2641 tacggttgtg aacaaagact ggatttgttc tttttcaagc cacttcgaaa taaattcgcc
     2701 agtgaaccct cctaaaaacc ctagtgctgt agattgagaa tcgatccgtt tcaaaatacg
     2761 ggaaacattg atcccttttc ctccaggtaa cttgaagtct tccttcattc ggttcaaatc
     2821 gcctagtttt aacccatcta cgcgtacgat ataatcaatt gatggattaa gtgttactgt
     2881 gtaaatcatt gtattgcctc cttaattgtt gtcttttcac ggaattcatt aacgaattct
     2941 aatggacaac gctcggttat gatcgtggct gcttccaaag gtgcaacaga ggtgaaggac
     3001 aactggttga atttggtatg atcaatcaat acaaatgtct cttcggaatt tttcatggct
     3061 aaacgtttaa cagctgcctc ttctgggtcc ggagtcgtga atccttgttc aagatgggca
     3121 ccgttcattc ccataaatgc tttattaaaa tgaaaatttt ttagttgttc tatcgcatta
     3181 gatcctaaaa cagcattagt tgccagcttt atcgttccac ctaaaataat ggtagcaagc
     3241 tccaaatcaa ctaaatttgc cgcatgttga acggaatttg tcactactgt gatctgtttt
     3301 ccttgcagaa aaggaatcat ttctaacgta gtagagccag cgtctaaata aatgacatct
     3361 tcgtcattaa tggtttcagc tgccgatttg gcgatttgct gtttttcgtg aacgtttttg
     3421 agtgattttt cactcatatt ctgctcaaaa ttcaataatt gctctttctt agctccgcca
     3481 tgaactcttt ctagaaaccc actattctcc agctcttgta aatcacgtct tatcgttgat
     3541 tcagaagcat taaaaaggtt tacaagatct tgtgatttca cgattttctg ctgttcaagc
     3601 aagtctagga ttttttgtct acgttcctct gtaagcattt tcacgatcac ctcaatacaa
     3661 ataatagcat attttttttc tactcgcaat ataaatcagt caaaaacgtt cataaaaatt
     3721 ataaatcagt caaaaaaaaa taaaaaacca gtcaaaaaga ctggtttttt atttttttgt
     3781 ctatcatcaa gccgttttat cgttttttat tttcgcttct tgtttttttc atataaacag
     3841 aagcaggttt tctttcctta tgaagaagat aataataaag tcccatcccg ccaaacatga
     3901 ccaaaaaacc gatgactgat cgaataggaa aattaggaac aaaaaagaga cagacggata
     3961 ataatgatgc cggaaccatt attcgataat agagttgttt atccattttc ttcacctcat
     4021 attttttgta aagtaatcaa ttgttctaat tctcggtaat ctaaaaattg cacacgcaga
     4081 tgctgctcac ctttcttgac atcgaattgg atacttcgcc gctttttctt tattcgtttg
     4141 aaggtaacat cagagagtct atgtttgatt tcctttaacg gtaattctga cagggattct
     4201 gaaggaacat agattcttaa ggataaaaga tactcttgat atttttcatc tgttacctct
     4261 acttctagat attccctaat ataataacaa taagcttcga ttccattatc caaagcaaca
     4321 gtcggatcga taactttata gcacatcatg cgcgagcgat tctcgtcaac ccaaggaaaa
     4381 tacgcctgag catttagtgt tccgcgaaca cgcctttctt tataatctcc ttgttgaata
     4441 tcataacttt ttgttacaaa aaatgaagca ttctttttag gaacaggttg catttgtgca
     4501 ttctgatact ggacagattg gatcgtatgg tcagtgtaaa tgtattcaac gaatggcggt
     4561 ttcaataaac cgacaccatt gtcccaagca agatgatcaa acccaagctg gtccctaaaa
     4621 taggattgaa acatttgatt gtttggcttt tgccagttaa aggggtctcg ataaagctgg
     4681 atacatatga cttcgttttc taattccttc actttttcta cttgttggat gtcgagaaaa
     4741 atcggtttag ggatgatttg atgataatag cgactataat acatttccca acacgaagtg
     4801 aaacataaac cttggtaaaa aagatcgtat cctggaaatt ggttgcaatc gactactact
     4861 tccccgtttt gttgtttcat tttaggtaaa tgttcgattt catccttttt ctcaaacaat
     4921 agccgttgag agatctgttt tgtattgtga tagataaact cgctataagc tcgcatatag
     4981 gcgaacaccc catgagcaat catttttttc tgaatgaagt ctcgaatcaa aaattgcttt
     5041 tctttgaaaa cagagataga caatacattt tttatcaata attgattatc ttccaagcga
     5101 aaactacatt cgttctcgtc atttcctaca actaaccaac tttcgtctct taccgatgct
     5161 agcattattt tttcttgaag ctttcgaaga tcatacggat ccgcttgttg tgtgaaagag
     5221 cggataaatg ttggctggat caacagacgt gtcaaaatag cccaatcgct tatttcctgt
     5281 tttttttctt cttcatttaa gtctgtgagc tggatagtca tcaataattt atcttcgcca
     5341 atcattttct cacctcttta aaatcttttt ataatttaat tataaccaac gataaagaaa
     5401 aactaaaact ttatgtctta gaaaccaatt tggacgatta catgatagac taagtaaaaa
     5461 gaaacaaaga ggtgtttaca atggggttga aatttgaacg tacagataat ttagataaac
     5521 ttttcgaatc atttgctgtc gatccggaaa aaacagttgc accaaaagaa gagatcgatc
     5581 gatttctaaa accacagcca caaaatgaaa agaaaaaaga caagaaacaa aagtaagtta
     5641 agctcaaaaa aagacttacc agccaaactt ttatgaaaga gtttgactgg taagtctttt
     5701 ttattctttt tatgattcaa ttgcttgttc gattgctgcc cacgcttctt cttttccctg
     5761 ttttgtaacg gaagaaaaga gaataaacgt gtcattgcga tcaaaatcca acgctttttt
     5821 gatttcgctc tcatgcttgt tccatttacc acgagggatc ttatctgcct tagtggcaac
     5881 aacaattaca gggatatcat aatacttcaa aaattgatac atctggatat cttcttgaga
     5941 aggcttatga cgcaaatcta ctaaagaaac gaccgcacgt agctggtctc tagtcgttaa
     6001 atatgtctcg atcatctttc cccattttgc tcgctcagtt ttggatactt ttgcataccc
     6061 atatcccgga acatcgacaa aatgtaatgc gttctcgatc aaataaaaat tcaatgtctg
     6121 ggtctttcct ggcttaccgg aagtacgggc taaattcttt cgatcaatca atgtgttgat
     6181 aaaagaagat ttccctacat ttgatcgtcc tgccagagct atttctggca attgtgtatc
     6241 tggatattgt tttggtgcca ctgcacttat gataatttca gcttgatgga ctttcatggt
     6301 ctctctcctt aacaaggtga tgaataaagc aagcggctgt cgcaaaagcc aaaatgactt
     6361 ttacggcagc ctcttttcat caacccgctt ttttctctga atctttatag attactgttg
     6421 gctcaccaga caattcagct gcttcttttg tgataatcac tttttcgatg ttttcatttg
     6481 aagggatatc aaacatcaca tccatcatga tttcttcaat gatggaacga agtcctcgag
     6541 caccggtatt gcgttcgatt gcttttttcg caatggctct caacgcttca ggttcgaatt
     6601 caagttcggt attatctaga gaaagaagtt tttgatattg tttgactagc gcatttttcg
     6661 gctcagtcaa gatccgtacc agatcatctg ttgtcaattt ctctaaagct gccataacag
     6721 gtaatcgtcc gataaactca gggatcaatc cgaattttag taaatcttca ggaatgatat
     6781 gctgcatgac actttcgtct tcagaaagtt tttggttttg tgtaccaaat ccaatcgtct
     6841 tttcgcccat acgattcttc acaatcgttt cgatgccatc aaatgcacca cctacgatga
     6901 acaatatgtt cgtcgtatca atctgaataa attcttgatg tggatgttta cgtcctccct
     6961 gaggtggcac gcttgcaaca gttccttcta gaattttcag caaggcttgt tggacacctt
     7021 cgccggaaac gtcacgagta atagatacat tttcgctttt acgcgcaatt ttatcgatct
     7081 catcaatata gatgatccct ttttctgcac gttccacatt gtaatcagca gattgaagca
     7141 atttaagcaa aatgttttct acatcttccc ccacataacc agcttctgtc aaacttgtcg
     7201 catctgcgat tgcaaaaggt acattcaatg tttttgctaa tgtttgtgct aaaaatgttt
     7261 taccagaccc tgtaggtccg atcaagcaga tattgctttt ttgcagttca acatcatctg
     7321 tgttttcttg tgcttgctga ttgacacgtt tatagtgatt gtatacggca acagctaacg
     7381 agcgttttgc gcgatcttgt ccaatcacgt agttattcaa tacttcaaga atttctagtg
     7441 gtttagggac ttcagtgaat tcgcgtacag cttcttcata aaattcttca tcaatgattt
     7501 ctttacatag gtcgatacat tcattacaga tgtagacccc tggaccagct acgatctttt
     7561 tcacttcttc ttgggttttc ccgcaaaaag aacagcgtac ggtctcattg ctacttggat
     7621 tgtcatacat ggtcttcacc ccttaacttg caaaaagtat gcaagaaact atcaaagact
     7681 ttacagtctt ttactatcat aacatagttt agtaaaaaga aaaagtgttt aacaaaggat
     7741 caactgacct atacctttgc attttttact cttaacaaaa aataaggaac tgtgacaaaa
     7801 tcgtgtcaca gtcccttatt tcgaataaac aattttctag aagtagcttc ttcagaaatg
     7861 atttattact tgatttctat tctttcgctt ctgtcgctgt acctgtgatc aattcgattg
     7921 cttttttcat tgtcacatca tgttccagca tatcttctgt caacacgcgt ttgatttgtt
     7981 cgattggcat gttgtatgtt tctgataatt cttggatttc tgcattgatt tcttcttctg
     8041 ttgcttgaag attttcagca gctgcaattg cttcgatcac aagatttgtt cttgtgcgca
     8101 tttctgcttc accttcgaat tgtttatgca agtcttcttc tgtagaacca gttaattgat
     8161 agtacatttc aggagagata ccttgacgtt gcatgttgtt caagaattcg tccattgaac
     8221 gatgtacttc atcatgaacc atcacgtgtg gcaattcaac gatttctgcg ttttcaacag
     8281 ccatacggat cgctgcttca tcttttgcat cgtccgctgc tttttctttt gtttctacta
     8341 attctttgcg atatttttct ttcaattcgt ctaatgttga tacttcgtca tcaacatctt
     8401 ttgcgaattc gtcatctaat tcaggcactt cttttgcttt tacttcatgg attgtcactt
     8461 tgaagactgc ttcttttcca gcaagatctt ctgcttgata atcttctggg aaagttacat
     8521 ttacatctag actttcacct gatttatgac caactaattg atcttcaaaa cctggaatga
     8581 atgagcctga acctaattca agtgagtaat tttcgccttt tccgccatca aacgcttcgc
     8641 catctacaaa gccttcgaag tcgattacta ctgtatcgcc attttcagct gcagcatctt
     8701 ctttgataac taattcagct tgtgcttctt gttcacgttt gatacgtgca tccacgtcag
     8761 catctgttac ttcacggtct tgtttttcaa caactagatc tttatattgt cctaatttta
     8821 cttctggttt cactgttact tcagcagtga ttacccagtc ttgacctttt tccatgcttt
     8881 caacatcgat ttttggttga gaaacaggat cgatgccagc ttctttgaca gcagcttcat
     8941 aagcttctgg taatacagca ttcaatgcat cttcatataa agcttcttca ccgtacatac
     9001 ggttgaatac ttgacgagaa actttccctt tacggaatcc aggtacgttc aagctttttt
     9061 tcactttatt aaacgcagtt gttaatcctt tttggatttc gtcttgcgca atcgtaaatt
     9121 ttaaaacacc atcattcgtg cctttttttt caaatgttgc agtcatttaa ttccctccga
     9181 gtttagaaat tcattttata catcaaaaac gtttcatgca tacctttaaa tagtacacta
     9241 accctttcaa attgtaaaca tattgaatgg atagaatcct tgtttttcat aacattttta
     9301 tctttttcac tgataaaatc taatggaaga agatcatttg ctgcaaaatt ttttctcttt
     9361 tttttaaaaa atcttgtttt tcatcttctg taccttcata catcgttttt cctgtacacg
     9421 cctctacagt cagttgaaac cagcgcttcg ggtcttcgat gtatgtatca tagacaggat
     9481 aaacaattgc agattgcaac gtgaattctt catttaacac ggaactaagc agcgcatctt
     9541 cattaccgta acgatctgaa aatacacata attgctggta acttgcctgc tgctgcggct
     9601 ttggcagatc ttttggtatg actttgacta acttttcttc aatcgtcaaa tagaaaacag
     9661 gatggcattc gttcaattgc ctaagtgtct ccagtaattt tgcccttgct aaaatatgga
     9721 cctctggcga tacaataagt ttactcgctg tttggataaa ctcatgataa ggaagctgtt
     9781 cgatctcatc aatagacgca agctgacgtg caggttccaa cgaaggaagt tcctccgatt
     9841 tattgtggag tgccttgatc ttctgcctct ccatttgtcc ggataattct tctgcaaatt
     9901 ggattttttc aatgacccgc tgctcgaaag cttcataaaa atctgtactt gctatcaatt
     9961 ttctagcata taaaaaatct tgtgtactga ttaacaagtc aaaataaaat tcagcaaact
    10021 cctcattttg aagatactgt ttttcatgca agagtgcttg cttcaatgct tcttttttct
    10081 cgtccaactc gtaaagacaa aaaacaagca ggctattagc ctcaaagctt tctttcaact
    10141 cgtaagctcg ttgaaataat tcttttgcct gctgatattg ctggtttgct agtgcttgtt
    10201 gtgctactcg cattaaattc tcatattttt ctggaaaatc aatcggcttc acgcagtgtc
    10261 ctcctcttgc tcatgttcag ctcatgttca atagtagcaa aacaaaaaaa gcatgacaat
    10321 taaaaataac tgaagcaccc tctaaaagct agattttaga tctgactttt agaaggtgct
    10381 ttcaactgtt actgcttttt attgctcatc agctgttgac ttcctggttg aatgctttga
    10441 tcagattgat cgtaacaaca cttgtattag tgaaaaaatc gcctagatgt tgctttttct
    10501 gattaaagat tgctggaaga taccctaaca tcagcaaagg aaattttaga atgaaccggc
    10561 aagcaccttc tctaactaaa acggttgacc agctaagttc ttcttcatca aagctgatga
    10621 ctcgaatacc aaagatcatt ttcccaatcg tttgaccatg attgaatttc gtcaataaaa
    10681 taaaatacgc tagataaata agtaaggctg tgaatccgta gatactcaga taagaatctg
    10741 aaaaattaag ccaacctaga tttttggcaa taccaagtgt taaacgcgta atagctccga
    10801 tacacaaaag atcgaccaag aaagcaaaca ttcggataaa aaaacctgca tagaaatact
    10861 taggaaaatc atttgctttt ggcggttcat ttcttgtgtc cgtatacttt ttccatttac
    10921 gttgtttctt tttgatttct tcgggcgtca aaggctctct ttcaaaagag ctcagtacct
    10981 tttgggaaaa gttggagtca ggtcctacag caggctgctg ttgcttatct ttttcttgtt
    11041 ccttattttc atttgaaagc tctgacatgc ttactcacct ccgtaataat acattgcttt
    11101 tggtgattcc gctgtcccta gattctcaag aacggataag atctgactag tctcagaagg
    11161 cttcaatccc tggaattctg ctagtttact tcctaaccat gtactagcaa acccagatgg
    11221 cgtggaagaa tattctacta gttccgcatc ttcaagctgt tgttcctttc gcatagcttt
    11281 taaagcatct tcaggaaatc ccaattcatc gactaaacct acttcttttg cttggacacc
    11341 atcataaatc cttccatcag caattttttt gacttcttct tcagatttat tccgtccctc
    11401 gctgacgatt gctacaaaac gactatatgc gctgtcgata tatgcttgaa gcaccgcttc
    11461 ttcttctttt gtctctggtc gcgttcctga acccatatct ttcaacgctc cgcttttaac
    11521 tgttgtatct tccacaccca gtttttctag aagtcctgaa taattcaatc ctgacatgat
    11581 cacaccaatt gagccggtca ctgtttcttc tgttgcaaag attttatctg cttgagcaga
    11641 aatatagtag ccaccgctag ctgccatgtt tttcatgctg acatacattg ggatatcatg
    11701 ttctttacga atcgtatcca atttcttagc gatttccgca ctttcgtaga caccgccacc
    11761 aggagaattt acttctaaaa agataccttt gactgttgga tcttcttcaa tcgccttcag
    11821 ttgttccata aaatttttat gattgtaacc ttcagaagca aataaacctg aagcccctcc
    11881 cgcagcaatc gttccatcta ctgataattt tacgattttt tgcgtactgt ctccctcttc
    11941 caacacattt gaagctagtt cttcatcccc atataaccaa ctgttcaatc cctcaatttg
    12001 atcgttttcc tgaggcttag tcaaactagc tgaaactaac gaaaccacaa ataatcctgc
    12061 agcaatcaga actgctgccc aacgtctctt attcattctt tctcctttct aatacaagtt
    12121 ttttatgacg atcacagttt ctgactgctc agaagtgtcg aaccgctctg ctttttcttc
    12181 ccgcgcatac ccggaaaacg actcttttgt caattcatct tctaccgtaa actcacgagc
    12241 ttcagcaact tccaaggcat caggtgcgat ttcttggatg ctatcccagc cagtatctaa
    12301 aaatattttc gtatcatctg atacaccagc tgatgcttcg aatttggaca attgtttttt
    12361 taacattccc agtgtcatga tctcaatttt tgaatccatt tttactcctc ctctatccga
    12421 ttactttcta ttgtaccgaa ctcctgaaaa agatgataga taaaaaccca ttattttttg
    12481 acataaaaag agcatttgac aaagcatttg atatatacca atttattttt tattcttttt
    12541 ttgttgacaa gaacattcgc aaaaggttat gattttaaat gtttccagat aaaaaacaaa
    12601 agaggaggat agttatacct tttaggatag cgccaggtct gtgtaaacag atgacgagga
    12661 gagggtttat cgaaagattc ggcggatggc cctagggact gcactctaca gaaaatccac
    12721 aaaaaatatc tgcaaagata aaacaaaagg attttcctgc agctggaagc attcatacga
    12781 attcattata gaaagtagga tgttttttat gtgcggaatt gttggaatga ttggattaaa
    12841 aaatgtaaca ccaggattga ttgacggtct agaaaaatta gaataccgtg gttatgattc
    12901 agctggtatc tttgtctcag atggcacaac tgattatctg gtcaaagcac aaggcagaat
    12961 tcaaaatttg aaaaacaaaa taaatattga tactacagga aatataggta ttggtcatac
    13021 acgctgggca actcacgggc aaccttctga agaaaacgcc catccacata cttcccaaag
    13081 tggacgcttc gtgctcgttc acaatggcgt aatcgaaaac tttgaagaac ttaaaattgc
    13141 ttatttagca aatgaccact ttattggtga gaccgatact gagattatcg ctcatctaat
    13201 tgaaactttc gcgaaggata ctacaacgca agaggcgttt ttaaaagctt tgcgggtcat
    13261 caaaggttct tacgctttcg ctttgattga tcggacagca cctgatgtta tttacgtcgc
    13321 taaaaataaa agtccactat taatcggact cggcgatgga ttcaatgtga ttgccagcga
    13381 tgcaatggct atgcttgctc atacgaagga attcgttgag attgaagatg aagagatggt
    13441 taccgtcaca tcagaaaaag ttattattca aaattttgca ggtgacatcg tagaacgttc
    13501 ttcattcgaa gcacaagtag attcttcaga tatcgagaaa ggtacctatc ctttttatat
    13561 gctgaaagaa atcgacgaac agcctattat catgcgtcgt atcgctcaaa aatacgtaac
    13621 agaagacaat cgtgtctctc ttgatcaaaa gctggtcgat acattgtctg atagcgatcg
    13681 aatctatatc gttgcttgcg gaactagtta ccatgcgggg ttagcaggta aacagactct
    13741 ggaaaaattg acacagatcc ctgttgaggt tcatttagcc agtgaatttg gctataacac
    13801 acctcttctt tcaaaaaaac cattctttat tttcttgagt caaagtggcg aaacagcaga
    13861 tagccgccaa gttctggtaa aaatcaaccg tctcggctac ccttcactta cgattacaaa
    13921 tgttgctggt tctactcttt cgcgggaagc atcctttaca ctgctattgc atgctggacc
    13981 cgaaatcgca gtagcatcaa cgaaagctta tactgcacaa atagctgtac taactttatt
    14041 agccaaagct atcggtgata aaaaagaatt tgaaacttct cttgtattcg atatcactca
    14101 tgaattgagt ttagttgcta atgcgatgga atcggtcatc gcccaaaaag attatttaga
    14161 agagttggca gaagtatatc tcagcgatac aagaaatgcc ttttacattg gacgtggcgc
    14221 tgactacaac gtttctcttg aagctgcttt gaaattgaaa gaaatcagtt atatccaggc
    14281 agaaggtttt gccgctggcg aattgaaaca tggaacgatt gctttgatcg aagaaggaac
    14341 accagtgatt ggaatcatca gtgaagaagt gactggtgcg catactcgag gaaacctgaa
    14401 agaagtcgaa agccgtggcg ctaaaacact agttattgtc tcagagggat tagaaaagga
    14461 aagtgaccag ctggtgctac caactgtcca cccttatcta actactctaa ccacagtcgt
    14521 accgactcaa ttacttgcat attacgcaac gctattacgg ggattcgacg ttgacaaacc
    14581 acgtaattta gcgaaatcag tgacagtaga ataataaaag aacaaggacc agtgtcagac
    14641 atctgacact ggtccttgtt cttttatttt ttatgaaaat atctcttaat ccagtccgat
    14701 ttcttctttg actactgccg cgattttatc gacatagtaa ttaactttct catcagtagg
    14761 cgcttctgcc atcactcgaa gtaatggttc tgtaccggaa ggacgaacta ggattcttcc
    14821 ttctgatccc atttcagctt ctgcctcttc aatcactttt ttgattgccg gaacatccat
    14881 cgcaccgttt ttatcactta ctcggatatt cactaatttt tgcggataga ttgttacctc
    14941 tgctgcaagc tctgataatt tcttacctgt ctgtttcatg atattcagta actgaattcc
    15001 tgaaagcatc ccatctccag ttgtgttaaa atctaagaaa atcatgtgcc cagactgctc
    15061 gccgccaaag ttataatcgt ttttgcgcat ttcttctaca acgtaacgat caccaacttg
    15121 tgtaacgaca tctttcaaac cgattccttc caccgctttg tggaatccta agttactcat
    15181 gacagtcgta acgatcgtat cttttttcaa tcgatttttt tctgccaagt atttagcaca
    15241 gataaacatg attttatcac cgtcgacaat gtttccaagt tcatccaccg caataatacg
    15301 atcgccatcg ccatcgaaag ctagtccagc atctgctcct ttttcaacga ccatctcagc
    15361 taaacgttct ggatgggtag agcctacgcc atcattgata tttaacccgt ctggatttgt
    15421 gcccatggta taaaaatctg tttcaagatc ggcaaacaaa cgattaacac ttgttgaagt
    15481 tgctccgttt gctgcatcga cacaaatggt caaaccggaa aggtcaccgt tgatcgtttg
    15541 tacaaggaat tgagaatatt tcaataagcc ttctggaaat tcttctacag ttcccaatcc
    15601 ttcagctgat ggacgaggca attcatccac ttcagcatct aataatgctt caatttctag
    15661 ctcttggtca tctactagtt taaaaccatc tgcaccgaaa aatttgattc cattatcttg
    15721 tgctggatta tgagaagcag aaatcatgac ccctgcgctt gctttttgca aacgtgtcag
    15781 ataagcaaca cctggtgtag agatcacacc tagctggaac acttcgatac ccaccgataa
    15841 aagtcctgaa atcagcgctt cttctaacat ttgacctgag attcttgtat cccgccctac
    15901 taacacgcgt gggcgttttc cttcttcttc atgctggctt aggacataac ctccgcaccg
    15961 tcccagctta aatgcaagtt ctggcgtaag ttccttgttg gcttcaccgc gtacgccgtc
    16021 tgttccaaaa tatttaccca tagttgtaaa attactcctt taacttaatt ttctgaggaa
    16081 tttgttctgc tcatttgtgt tgattcgctt gtttctgatg tttcttctgt acttgtggtc
    16141 gagctgcttg tcccagattc cgaactggaa gaaatggttg ttgtcgatgg ttccgttcct
    16201 gaatcagcag atgaaatact tctactcgta cttgtatttt cttgtgtccg actgctcgtc
    16261 gaagcagagc tactgcttga cgtttgcaac acgggtgtaa tctccacact aactgattcg
    16321 ggcttgatac ttaacccctc ttctactggt atttcaattt tacgagttgt tcgatgattg
    16381 atacctgaga cattgatcgg tacagcaata ctgtcaccga tcgatgcaag caaatcgcta
    16441 gagccgctta cttctgcttc cagttttgaa aaactgaaat catagctttc taccccggca
    16501 gctggtgttc cttgctgtat cccataaagt cgtaccgact tgcttggctt gatcgaatca
    16561 gctctcactg ttacttgcac tggatcagag acgatactta atacttcccc tgctgcattc
    16621 agcgcctgta ttgggacctc actattaatc ccatcatctg tgaccatcga agcgtccaca
    16681 tcagctacta ctcgtttgat ttctgccaat gttttgtcac ctgtagtgat agagactttt
    16741 ttaggactaa cggaagtatc atcaagttca tatccagatg gtgtgaacgt tgaaggaatc
    16801 aacgtttcca catcgaaatc ttttattacc tttttctcaa ccgttactgt aatagtcgat
    16861 ggttcgatct ttgctgtgac agcactgctc aaattttgaa tttttagcgg aacctcatgt
    16921 gttccttcgc ccagcttagt gatatctgct gtgacgcgga acatccgtgt atcttcgttt
    16981 gcctctgcat tcaactgaac tcggtttgca ctggaaagtt tgacagtcac tgcattttca
    17041 tatccatgga tatagtagcg ctttgaatca taagaaatct tgattggcac cccagtaact
    17101 gtttgattat acgcttcatt tccagaaagc gtatttggaa cggactggcc atttgcattg
    17161 aagtacagcg caatactgaa cagcaatgca ataaaactgt aaagaatgct tgtccgtttc
    17221 tcttttgaga tcatttgcga tcccctccct tagtcatacc gtctaggaaa gattgaatca
    17281 cgtttttctt gttttttgtt ttctcttcag caatcagttc tctacgcaaa attgccaaat
    17341 attcttgctg ggataagcca gccatcagtt cattatctaa tgtaatgctg acatccccag
    17401 tttcttcaga gacaacgatg gtaatcgcgt cgctgacttc gcttataccg acagctgccc
    17461 ggtgtcgagt cccgaattct tttggaatca acatactttc tgatagagga agataggcgc
    17521 ttgctactgc gatctttcct tcttttacaa taactgcacc gtcatgcagc ggtgtattag
    17581 gaataaagat attgatcagc agctctccag tgatatctgc atctaatgca attcctgtct
    17641 caatatactc atctaatcca gtatgccgtt cgatagtaat tagcgcaccg atttttcgct
    17701 tggacatata ttggatggct ttgtcaaaag acaagatcat cttctcatct tcctgctgtt
    17761 cgctttttga ttgtttgaaa agcgaactgc gtccaagatg ttccagtcct ctccggattt
    17821 ccggctgaaa aatcacgata gcagctatca ccccgtacgt aatgacttgg ttcatcagcc
    17881 aagacaaggt gtgcagacca atgatttccg ctacgattcg gataacaata aaaatagcta
    17941 cacctttcag aagctgaatc gcttttgtgc ctcgcagcag ttggatcagt ttgtatacaa
    18001 gataccagac aaccagaata tcaagtatgt tcaccaaaaa attcagagag aacaagtcac
    18061 cggaaaacat ttgctgccaa tagtccaaat taaaaagttc cccgatttga aatgacataa
    18121 aaagcctctc ttctcttttg agatttcctc tctagtatat catatgtttt aattttagag
    18181 gctgatctgc tcgtattttt cttgagaaaa acgcataatt ttcaggaaaa ttgcaagtat
    18241 gcgttttatt tttctctgca ataaatattt ttaaacttca gagaattatt cattatattt
    18301 tttctgttat ttttcctttt tttcttttgt agcttgttcc atttttttac gtcgttctgc
    18361 tctggcagct cttctttctt ctgcttcaag gtccgtcgtt ttttcttttc tagtcttaac
    18421 gggtacatcg atagattcct ctggttcaag tcgtttcttg attttttcta attctcccgt
    18481 cagtgcagca tagttgtaaa aacgtgtttt agcgtcttgg atagtctttt caaataaagc
    18541 ttttgcaaga tttggatttg cgttctctag cgaggaaaag cgaacttgtt tcctcatgaa
    18601 atcaggcata gcagagaaat caggtttttt gtagtccaaa gtcattgggt tcttgttttt
    18661 ttcgaccatt gcagggttat atcgatataa tgaccaatag ccagaagaaa cagcttcttt
    18721 tgcttcttta agcgattctt tcattccacc aagcaaacca tgtgtgatac aaggcgtata
    18781 ggcaatgatt aaagaaggtc caggatactt ttctgcttct tcaaaagcct ttatcgtctg
    18841 catttgattg gcacctgaag caatttgcgc cacataaaca tttccatatg tcatcgccat
    18901 cattcctaga tctttctttg aagcatactt tccactagcc gaaaatttag cgattgccga
    18961 agctggtgtt gcttttgagg tttgaccgcc agtattggaa tagacttcgt tatccaaaac
    19021 aagaatattc acgtctgctc cgctggcaag tacatgatcg atcccgccaa aaccaatgtc
    19081 gtaagcccat ccgtctccac caatcatcca ttggctgttt tttacgaata gatcctttct
    19141 cttgtatata tcctccagtt cagggacaat tgatcgttcg ctttcgatcg cagcttgcag
    19201 ttttgctgct ctttgctgcg ttccttcgct ctcaaacatg tgtaccaacc agtcttccat
    19261 caattctcgt agttcttctg atacttttgg caatacttct ttgattgaac ttgccaaagc
    19321 ctcgcgacga gtctgtgccg ctagaagcat accataacca aattcagcat tatcttccaa
    19381 taaagaattt gaccatgcag gcccttgacc acattcattg gtcgtataag gagaagcagg
    19441 agaagcaccg ccccaaatag aagaacagcc tgtcgcattc gcgatcatca ttcgatcacc
    19501 gaacatttgt gtcaataatt ttacataagg tgtttcgcca cagcctgagc aagcgcccga
    19561 gaattcaaga agcggttgat tgaattgtgt agacaagaca gtattctttc ctttgactgg
    19621 atttgctttt tgtcttaatg tcattgcaaa agcccaattc acagcttgtt ctttctgttc
    19681 ttcataaggc ttcatcacta aagctttttc ttttgcggga caagcctcta cacacagacc
    19741 acaacctgta cagtcttcca ctgaaacttg gatacggtat tttacgccgt ctgcgcctcg
    19801 catttctctc acgataaatc cttctggcgc ttcttccatt tcctcttcat ctgctaaaaa
    19861 tggacggatg gcagcatgag gacaaacaaa ggcacattca ttacacatcg tacagcgatc
    19921 actaatccac tcaggtactt ctaaagcgac gccccgcttt tccatagaag ccgttcctaa
    19981 aggcatttcg ccagttgtca ttccatttgc tacaagtgta ccaacagaga gttgatttcc
    20041 ttcctgtgca ttcactggtt caagtatttc ttgtatataa ccagtgttcc ttttattgat
    20101 tttttcaggc atctccaacg ttctccaatg gtctgggatc gcgacttgat gaagcagttc
    20161 ggtcgtccga tcgattgctt gtatgttttt ttctacgata gccattgatt tatgtccata
    20221 gcttttcagg gcttcttctt ttagcagcgg aagcacctca tcaaaaggaa tgatgtcagc
    20281 taatttaaaa aaagccgttt ccattgccgt attgatccgg cgtcccaaac cgacttcttg
    20341 ggccaatctc atggcattga tcgtatagaa tctaatgtta ttttcagcca agtaacgttt
    20401 taattttaag ggcagatgcg tttctaattg ttcgtcgctc caaagagtat tcaataaaaa
    20461 aatgccacct ggcttcaatc ctttgaccaa gtcatagcta tgcaggtaag ctggtgtgtg
    20521 acaggcaaca aggtccgcat gctcgacaag atacgctgaa cgtatcggcg tatctccaaa
    20581 acgaagatga gagacagtca gaccgccaga ttttttcgaa tcataataaa aatagccttg
    20641 cgcatattta tcggtgtgat ctccaataat cttgatagct gatttatttg ctccaactgt
    20701 tccgtcagat ccaaatcccc aaaacttggc ctggaatgtt tgcggttctg tcaaatccag
    20761 actttccttt ggttccaaag attgataagt cacatcatcc acgatcccta tggtaaaccg
    20821 ttttttgcgt atgcttgggt cttttttcaa ttcatcaaaa actgctgaga tttggtcggg
    20881 agtcacatct ttagacccca atccgtagcg accaccaata acagccgggc gaagttcaga
    20941 atcatataac gcactttgaa cgtctaataa gagaggttct ccacctgccc ctggttcttt
    21001 acttcgatca agaacagcaa ttgtttttac tgtatgaggc aacttttgca cgaatgtttc
    21061 cactggaaaa ggacgataaa gatggatatt caaaaaacct gttttccgtc cgtttgcatt
    21121 caagtaatca atcgtttgtt caatcgtctg tgcaactgat cccatagaaa caatgacttc
    21181 ttccgcatcg tctgctccgt aataagtcac caagtcataa gaagttcctc gtaatcggtt
    21241 gatctctttc atatattttt gaacgatttc aggtaattta tcataatagg ggttgatcgt
    21301 ttcccgttgt tgaaaatgga tgtctggatt ctggttcgta ccactaacag tcggatgatt
    21361 gggattcatc cctcgtttac gaaatgaagc taatttctcc tgattgatca gaggagctaa
    21421 ttcttcatat tccaaaactt cgattttttg aatctcgtga ctcgtacgga atccatcaaa
    21481 gaaattcaaa aaaggtactt ctccttcaat agaagcaaga tgggcaacag gtgctaaatc
    21541 catcacttct tgtacactgc tttcagctaa catagcaaat cctgtttgtc ttgcagccat
    21601 cacgtctccg tgatctccga aaatattcaa cgcatttgtc gtcactgccc gactagcaac
    21661 atggaaaaca cttggcagta gttccccagc aatcttgtac atattcggta tcatcaaaag
    21721 cagcccttga gaagcggtat atgtagttgt cagtgcacca gttttcaatg atccatggac
    21781 cacaccagct gctcctgctt cggattgcat ttctactact tttactggtt gaccgaatac
    21841 attttctttt cctgctgctg accattgatc tactaattca gccatggtcg aactgggtgt
    21901 tattggatat attgcagcaa tctcggtaaa tgcataagaa atatatgcgg ctgccgtatt
    21961 gccatccatt gttttcattt ttcgcatgat tcatcatcct tgttcatatt ctctcttatt
    22021 atctgttcca ttataacgta aaacattcca ttttttcttt tatttataaa aatatttcac
    22081 atatgtctat ttgaattggt catctaattt ttagacaaac agtctaaatc gttgcctttt
    22141 tttgtcagac atgtataaac tataagcgaa agaggtgttt taacatggat atatcagtga
    22201 ttgatgcaac gaaaacaaat acacaaaaag gaatccttta tggttcatcg aatgctccta
    22261 aaaaaatggt cgaattcatc aacctatctt gtccgtattg ccgacaatgg tttgaagaat
    22321 cttatgagct attagaagaa gcagtccaaa gcgggcaatt gcagcgagtg atcaaattgt
    22381 tcgataaaga aaaagaaagc ttgcagcgag gaaacgtgat gcaccggtat ctcacaatca
    22441 gcgatggtca aaaagcgatc aaagagatca aacaaatatt cgatactcaa gacgagtgga
    22501 aacatctttc tctccaagaa gtagcggatt ttgcagtaga taaactaaag ctgacagaac
    22561 aaaaagacga acagctgagc caagctgtca tcaacgaagc tgagcaagca catattcgat
    22621 ttgttccgac tgtcatcttg ggtaaagaaa tctttgatga atctatttca atagaggaat
    22681 taaaagaatt gatccaaaca aaataaaaaa ataaggagcg taggacaaaa gtaaagaaca
    22741 cttttgtctc acgctctaaa cacctataaa cggcggaagc agaagcaact ccttcttatt
    22801 tcttggagtt aaacgcttct gtcccagcct ccttattccg aataaatggt gtccaaaagc
    22861 ttgcatctgc cagattatct catttcttat gacaagctga gcaaattatc tccaccaaat
    22921 aatttattca tatttttgac ttgcgcatac taactaattc ttaaattttc atacaaaatt
    22981 tctcaaattc ccttaacgag taatatttat gtacggtgat tcacactagt atcccaatca
    23041 ctttttaagt tatcccgatt tcaattctac ttcaaaaata gttgctttta tgtatgctgt
    23101 tagttgatta gatggtcgtt tgttgagcta ggtcgtttat tttaaacaag ttgtttcttt
    23161 agcttatacc tatctcgtct accaaacgca agatggcaaa cacaaatgca tacgcatctg
    23221 tatttgccac tttgattcgt ataaagctaa caactcctaa ctcctctcat ttaatagttg
    23281 ctctcttttt tattcagata catttttgct gatcacagcc tagtatttca atggttttga
    23341 acactaaacc aaattgttcc attacaacat tcactggctg atgaaatgga ttaatagatt
    23401 tcgattattc aaagcgattt tctattggtg aattgtgttc ccaactaaca agacggattc
    23461 caaagtcatt atataactct tcaagagtta accctttcct gttcccaata gatgtatctc
    23521 tcccacgctc gatataaaat atggagtttc gaatgccaag acctggcaaa tctttccttg
    23581 ttacagttac actgctacta acttccccat ataaaatcca gctcctccaa aatgataata
    23641 tatttttcga ccatcatcta actcatgtaa caataattgt tgtgccctct tgtcactcgc
    23701 caaatgcaaa cgccacgtgc gataccatgc catgctacct gcatctgcag aaaatccatc
    23761 aaataatgca acaattgtat cttctatcac aatcctttcc ccatctactt catattctaa
    23821 tctaaaatca aactcccctc gggtaatcgc aggtcttggt ggcttaggac ccaatattat
    23881 tctacttaaa accgcattcg ttcccagatg aaacacagct ataacaacaa taatgattaa
    23941 tattttgttt ctatttgata aatttctcat tattcgcaaa tcacctctac attctcacat
    24001 actagttcgt aatactcctt taatatcaaa atatctactg atcgtaataa tattcgctat
    24061 gaattcctac tactcgtata agaaaggcca aatcatcagc ttttacttgc tgatgatttg
    24121 gcttgtttct atttatcgta attgttttct aatcaccaaa attgttaaca atcggttcag
    24181 atatttccca attcactagc cttatattat attgatttaa caattcttct tcatttattg
    24241 tattttgatc aagcggacca aattgttcta ataattcttt gtcatgtaaa atatcccttc
    24301 tagtaatgcc tctttccctt tcaatgaaag aagcactagg aaaagtatga ttataaccat
    24361 tcaacctatc tcccatgtaa taattagcag gtcctggaaa ataaaatatt tgcttattat
    24421 cactaacttc taacagcaac actaaatctt ctccgctggc taaacgactt gtccatctac
    24481 gatatctccc tatgccttcg ttcattccaa atccatcaaa ttcaactatt aacgtgtctt
    24541 ctatcacatg taattcttca ttcaattcat actcaagtct aaaaggaaat tctccatatg
    24601 taatctctgg caatggtggt gctggagttc tccaaataaa tacaataact gtgaatagaa
    24661 aaaatccaac gatcgatata aatgttatga gacctgctcg aagtaaaacc cgatataata
    24721 aaaatgctgt atttcgcttt gctatatcta gaaaaataaa aagtactgca atcataatga
    24781 tagtaaaagt tgtaaaaaaa cgcatttttg ccctcccttt ataaacatcg attctcaaat
    24841 tctaaaccaa atcttggtag ttcaagttca ggtataacgt attccaacca tacatctcaa
    24901 ttactattta tgaacttatg attatcactg aataaatcac tccatcccct tgttaaaact
    24961 tctacattaa ttacacctgg ttgttttcga ctagctaatc caatgaactt atcaaccatc
    25021 cttatccacc aaccacgaga atcttgacca gaagtaagaa gagcagttct caaggatgat
    25081 cgagcagttc cttccagttt tttaattaat caggccagtt atagataaat acttgatctt
    25141 atctatatca ggacttctat attaagcaaa aatttatgta caaaactaaa atcttcattc
    25201 agttgtctcc ccgcttttta tacgattgta ttaaaaacaa tagaagcctt tccaaattat
    25261 gttactccag taaagatgga attatagttt gacggaagaa aagcaagtat aacttttaaa
    25321 aaaaactgat acattcgcat ctcctgcttc atgtatcagt tttttctttg gattaaactg
    25381 cttcttcacg cagtttttcc gcaaattcat ttattttgcg aattcggtga ttaattcccg
    25441 attttgatat cgcacctgag ggaatcattt cccctaattc tttcaagctg acctctggat
    25501 gttgaagccg aagttctgcg atttcctgaa gtttttcagg aagggcatgt agaccgacct
    25561 ttgcttcgat caattcgatg ttttcgattt gtttagaagc cgcatcaatc gtcttgttca
    25621 gatttgctgt ctcacaattg actagacgat tgaccgaatt tctcatatcc cgtacgattc
    25681 gaacatcttc aaatcggagc attgagttgg ttgcaccaat caatgttaag aaatcagcaa
    25741 ttttttctgc tccttttaaa taagaaatat agccattgcg ccgttcaagc gttcgagcat
    25801 gtagaccgta ataatttaac atatcgcaaa tgtcttggtt atgctgttca taaatagaaa
    25861 atatttccaa atgataacgg ctcgtttccg gattattgac tgaacctgag gccataaaag
    25921 caccacgtaa gtaagaacgc attttttgcg aatttcccat aatctcgtcc gaaacgtgtc
    25981 cattgaacat cataccatcc atgatatcaa gatccatcaa cacatctttt gtgccttgct
    26041 tcaaccggac gatatagaca ttgttctttt ttaatttcat ttttttacga acgagtagct
    26101 cacttcgtac ctgataatga tctttcaaaa gagaatagat acgtctggca attgcagcat
    26161 tttctgtttg gacgttcaaa ataaactgct ggttcacaat acctaaagag ccgttcattc
    26221 gcagcaaagc agctaattcc gctttcgcat gttctcgatg aacttccaaa ccagttagtt
    26281 ctttcttcac atctgcagca aatgacatcc tattctcctc ctttctccaa caataaattg
    26341 cttaatattt tgaaccgaaa actaaacgga acaattcttc tactactttg tctccatcgt
    26401 ggaagacacc accatctttc aattccaaaa agtcggtgga aatcactcga catccttcat
    26461 ttctcaatcc gttgaagtcg tgtgcgactt gtactagata ctcatcatag atctctgggt
    26521 ccatataatc ttcaggcact ttttccgtat tgaccaatac tgtatcgata aaaggctgat
    26581 tcaaatgctt gtgtaaaaca cgtatatgat cggcatctgt gaaatgttcc gtttcccctt
    26641 tttgggtcat gatattacag atatagacgg tttcagcagt tgtcttgagc attgcttcgc
    26701 cgatttctgg tatcactaaa tttggaagga tactggtgaa taaacttccg ggtcccaaaa
    26761 cgaccatgtc ggcgtccata attgctgaaa cgacttttcg agccgctttg gcttcacttt
    26821 tatcatttgt gttgcagaca tacacacggt caatggtttt acggtcgatc gcgattttag
    26881 attctcctac tgcagtcgtt ccgtctttaa agacagcatg caaggtcaaa ggagtttctg
    26941 aagaaggata aacatgtcca tctacatgca tcatttttgc taataattga atcgcctcat
    27001 aagtgcttcc ccgcatctcg gaaactgcgg caatgatcag attaccgatc gtatgattgg
    27061 ctaaaaactg atcttccttt ttgaaacggt attggaaaat atcttcatag aattgtggca
    27121 tatccgataa cgccactaag acatttctca agtcaccagg aggagccata gcgatcgatt
    27181 ctctgattgc accgctgctt cccccatcat ctgcaacagt cactactgct gtgatatctg
    27241 ctccttgatt tcgcaaactt ttcaggataa ctggcagacc agtgccaccg ccgaccacta
    27301 cgatctttgg ttttcttatc cggtaggttt tcattttcac gaccggttca ccgtctcttt
    27361 ccttttatcc ttgtcccgat gtgtcacatt gactttatag tttttcccta aagcatttgc
    27421 caaacgttca gtcaatgcca cagaacggtg ttgaccgcct gtacagccga ttgcaactgt
    27481 caagctgctt tttccttctt taatgtatcc aggcaataca gtttccagaa gatccaaaaa
    27541 tttatggtag aatgattgcg tttcatcaaa ggacatcaca taatcataga ctggctgatc
    27601 ttttccggtc aatggtctta gttccgggat ataatgaggg ttgggcaaaa aacgcacatc
    27661 catcacaata tccgaatcga tcggcaaccc gtatttaaaa ccaaatgata caaattcgat
    27721 ccgaaatcct tggtctccct ttgatttgaa tgactcattg attttttcac gtaattgtcg
    27781 tggtgtcaat gtcgtcgtat caatgatcac ggaagcttgg gctttcaagt cgtctagtat
    27841 cgcccgttct ttacgtatcc cttcagtgat caaaccgtcc atcgccattg gatgtgctcg
    27901 tcttgtttct ttgtagcgag ccactagttc ttcgtctgaa gcatctaaaa ataagataga
    27961 agtgtcgatg aagttcgtat tttcgatttc gatcagcatg ttttggattt cttcaaagaa
    28021 ggaacgagaa cgtaaatcca ctcctaaagc catttttgtg actttgcctg attctttgat
    28081 taattcccag aattttggaa tcaatcgagg cggcatatta tcgatacaaa aatagcccat
    28141 atcttcaaaa ctttggatag ctactgtttt ccctgcaccg ctcatacctg ttatgatgac
    28201 taattgcaaa ttatctgtca taattacgaa ctcctctcac attgtgcata gtaaaattat
    28261 atcattccgg gcgtatcatt accagttttc tttttctttt acaaaaagat ttggaccaaa
    28321 tatcttttag gatatttgcc ccaaatccaa tttttttact tttccgatgc tgtatccaat
    28381 gccttgcttc tttcagctag cgctcgttca atcgtccgat ctgctaaaat agattgggca
    28441 tcgatcactg gataatggtt atcttcagca aattcatgca tcaatgacaa ttctgtgcaa
    28501 cctaagataa ctttttcaca gtttaggcgc tctaccgctt cttctaagat ttcgtaatat
    28561 agctcttgat tcaaataatc cgattccttg atctcatgat agatcagata attgattttt
    28621 tcttgaagaa cagtatcagg aatcactgtt tcgaatccaa gatttttgac ttcgcgttca
    28681 tagattccag ctttcatact tccttctgtt cctagaatcg ctactctccc tgtcgtatgc
    28741 tgacggacaa gttcatttgc tgcttctctt ggcatatgaa ggataggaat atccgttgcc
    28801 gcttgtaatt cttcaaaaaa gtagtgtgcg gtgttacaag tcaacacaat gaagttcggc
    28861 tgcaataaat tttgtttctc aatatcatcc agcagaaacg gcattggatt ttcttctgat
    28921 cgatctaaaa tataagctgt acgatcagga actgttgcat gattgaataa gacataattc
    28981 aaatattctt gatcttttgt agctttcgtt cggtggttga tcaagcgaac aaaactttct
    29041 gtcgccatcg tgcccattcc gcctaaaata ctgaaaaaat tctccatgaa ctagccttct
    29101 ttcacatgaa aatacttttt gaatcttgga atatagttat ggaacatata tttcatcaac
    29161 aaccagcgct tgattgaccg gtctttttca taaaacacag tagtaccgta acgattttct
    29221 ttgatcattt gctcagctag ttttttgtct tcattttccc gagcatattc taaaaagatt
    29281 tttttgggca cacccatcca taatcttgct ttatctgatt gattcgttcc ataggtcgtt
    29341 tcaacaaagg gtttgttaaa cactcgatct tctgtgacga aacgagccag attcaagccg
    29401 ttcaatgtca caaagaaact gctacgacct tgtctcaaat tgatttcaaa taatttatat
    29461 tctccatctc taggatcata cttcatatcg aaattggcaa agcctgtata ttcgattttt
    29521 tccaaaaatg ccttaatggt ttgatagatt ttttcgttat aatctggcat gatcacaacg
    29581 taattaccaa tcgatgctgg tgtaggatct tctaacagtg gatgaccaag acacatcatc
    29641 cgaacttgat gatcttcatc tacatacgca tttagcacac gcatattact atcatcaccc
    29701 gggataaaat cttgtacaat catttcactt ttataaccgg cttcgtatat ccggcctaaa
    29761 attaaatcga actcttctct agtctctaaa ataaaagctt tttttcttcc ttcaaattgg
    29821 acagaaagat actccacact gtttgcaggt ttcaaagcaa ctgggaattc aaaaggcaat
    29881 tcctgttcca aatgtccatt tactaacatt tcttctctga cgatcaatgt tttaggatac
    29941 ggaagatcat attcttcaca aacttcataa aagcttactt tattgatcag tctttcaaat
    30001 aaagaataat caatatatgg acagataaaa tattctgaca gctcttgttt atgttgggag
    30061 atcaattctg catatccatc accacaagca atcaaaagat attttttact cttatcagaa
    30121 tatctttctt ttgccaaacg aagcatcgtc tcgataaaca cagggtcttt atcaaatcct
    30181 ggaataactt ctacattgac aattttactg tagcgggtag gggctaactg atccgaagca
    30241 tatgcttggc agattttgcc gtatgcttca ttaaaagaac gtgccatacc ataaacgttc
    30301 atgtcgcttc ctaataatat agggataaat tcttcatttt caatactgtt catcattatc
    30361 tctcacttac tcataatctc ttgtttttct tttccaacta cacaaggaat atcataccat
    30421 aaagaaaaaa gcaatcaaag gaaactcctc cgattgctca attctttttt gaacggattg
    30481 aacttatagg aagtttttgt ctataccaat cagtgtagaa agaactacca tgtaatgatt
    30541 ctagaacact gatattaata aaacaagcaa aaattcattt ttttatgttt ttttaactat
    30601 ctcagctatc tatcctcttt ataccattat ttttttcaaa tagtgacctg tataactttc
    30661 ttttacgtta atgatttctt ctggtgttcc agtcgcaagg atcgttcctc caccttctcc
    30721 accttctggt cctaaatcaa tgatataatc tgctgttttg attacatcta gattgtgttc
    30781 aatcactaaa actgtgttac cagcatctac taatctttgt aatacatgca acaagcgggc
    30841 gatgtcatcg ctatgaagtc ctgtcgttgg ttcatctagt atatagaaat tctttccatt
    30901 agagattttg tgcaattcac tggcaagttt catccgttgt gcctcaccac cggacaatgt
    30961 cgttgctggt tgccccatcg tcacataacc taagccaaca tcaacaatcg tttgcagttt
    31021 gcgatgaatc tttggaatgt gcttgaagaa ttctacagca tcttctactg tcatttccaa
    31081 aatatcagca atgctttttc ctttgtaatg cacttctaat gtttcagagt tatatcgttt
    31141 gccatgacat acttcacaag gaacatagac atcaggcaaa aagtgcattt cgatcttaat
    31201 aattccatcc ccgcgacaag cttcacaacg accgcctttt acgttgaaac taaaacgccc
    31261 tttcttataa ccccgcattt ttgcctcgtt cgtttgagca aataacccac ggatatcatc
    31321 aaatacactt gtataagtcg ctggattact tctcggcgtc cggccgattg ggctttgatc
    31381 gatatcgatg atcttttcga tactttcgta gccggaaatt gtcttgaatt tacctggctt
    31441 agcagaattc ttatttaatt tttgcgctaa cgatttcttt aagatactat tgaccaatgt
    31501 actctttcca gaaccagaca cacctgtgac tgctacaaat tctcctaatg gaaattcaac
    31561 actaatattt ttcagattgt tttctgaagc accagtgatc ttgattgctt tcccattacc
    31621 tttacggcgt tcttttggta cagggattga ttttttccct gaaagatatt tccctgtcaa
    31681 agaattttca tcattagcta cttcttcagg agtgcctgca gccacgattt gtccgccttg
    31741 ttctccggcc ccaggtccga cgtcaatcag ataatctgct gcccacatcg tatcttcatc
    31801 atgttcgact acaacgaggg tgttgccaag atcacgcatt ttctttaatg agccaatcag
    31861 tcgatcgttg tcgcgttgat gcaatccgat cgatggttca tctaaaatat acaaaactcc
    31921 agaaagattc gatccaattt gtgtagccaa tctgattcgc tgcgcttctc ccccagataa
    31981 tgttccggaa gcgcgactca aagttagata gtctagtccc acattttcca agaatgaaag
    32041 tctgtcattt acttctttta aaatcggttt ggcaattact ttctcttgtt cagataacgt
    32101 taagtttgag aagaaatcta ctgctttttg aatagacaac tcatttgttt gcccgatatt
    32161 cgttccattg atttgaacag atagagcttg gggattcaat cgataacctt tacatgtctg
    32221 acaagttaat tctgtcatat aaagacgcat ttgctcacgc gtgaaatcac tgttcgtatc
    32281 atgataacga cgctcgatat tcttcaatac cccttcgaaa gtcgtttcta catcgcgaac
    32341 gccaccaaaa tcattttcat aatggaaatg gaaaagatca tctgtcccat tcaagatgat
    32401 atcctgatgc tcttccggta attcttcgaa tggtgtatct aaatcgatac cgaaactttc
    32461 acaagcctgc gctaacattt gaggataata ctgagagctg attggattcc atggaacgat
    32521 agcaccttga ctaagtgtct tcgttctatc tggaatcact aaatcttgat cgacttctaa
    32581 tttgacacct aaaccatcac agtctggaca agctccaaaa ggcgcattga atgagaacag
    32641 tcttggttct agttcgccaa cagtaaatcc gcaataagga caggcatagt gttcgctaaa
    32701 caacatttct tcttcaccga tcacgtcaac cagagcatag ccgtctgcca atcgcagagc
    32761 cgcctctaaa gaatcaaata gacgagaacg aatcccttct ttcacaatga tccggtcgat
    32821 gacgatcgcg atatcatgtt ttttgttttt ctccagctca ggcacttcac ttaaatcata
    32881 gatctcacca tcgactcgaa cgcggacgta tccctctttt tggattcgtt cgaatatttt
    32941 tttatgttgt ccttttttct ttaccacgat cggtgccaac acttggatct ttgtgcgctc
    33001 aggcaaagcc attacttgat ctgccatttg atctactgat tgactggtaa tctcgatatg
    33061 atcgttggga caaatcggat gtcctacacg tgcaaacaac aagcgtaaat agtcattgat
    33121 ttccgtcaca gtacctactg tcgaccgagg atttttactt gttgttttct gatcaataga
    33181 aatcgccgga ctcaatccgt cgatgctatc tacgtccggc ttatccattt gtcctaaaaa
    33241 ctgacgagca taagcagaaa ggctctctac gtatctccgc tgcccttcag cgtacagcgt
    33301 atcaaatgct agggaacttt ttccagaacc ggacaaacct gtaacgacca cgaatttatc
    33361 tcgtggaatc gttacatcta tatttttcaa gttatgggca cgtgcaccgt gaatcgtaat
    33421 tttatcgtta gccatgttgt cctcctactt tgaagctttt aattctaaaa tcatatctcg
    33481 cacattagcg gctgtttcaa aatccaatgc tttcgccgca tttttcattt ctttttctaa
    33541 tttcatcaat aacgtatctt tttcttcttt cgtcatttct tcatacgaga cttgtaccgc
    33601 ttcttttgta tcatcttccg attctttcgt aatagaaatc aaatctcgga tttctttaat
    33661 gatcgttttt ggaacgatcc catgttcttc attgtacttc tgctgaatcg tacggcgtct
    33721 tgatgtttca tccatcgcta gacgcataga atctgttact ttgtctgcgt acatgattac
    33781 tttaccttct tcattccgag cagctcgtcc aatcgtttgg actaaggaac gttcactacg
    33841 taaaaagcct tctttatccg catccaagat agcaacaagg gaaacttcag gtacgtctaa
    33901 tccttcacga agcaaattga ttccaactaa cacatcaaat tcacctaatc gaagatctcg
    33961 aatgatttct gttcgttcca gcgttttgat atcactatgc aaatatttga ctttgatccc
    34021 taattctttg aaataatctg tgaggtcttc tgccattttt ttcgtcaatg tcgttacaaa
    34081 cacccgctgg tctttttcga ctcgttcatt tatttcgcct actagatcat cgatttgtcc
    34141 catgattgga cgaacttcga tcactgggtc aagcagccct gtcggacgga tgatttgttg
    34201 gataacggta tctgtttgtt catgttcata tggtcctggc gtcgcagaga cataaatgat
    34261 ctgattgaca tgtttttcaa actcttctaa ccgaagtggc cgattgtcta aagcagatgg
    34321 caaacggaaa ccgtaatcta ctagcatctg ttttctcgct cggtcaccat tatacattcc
    34381 tctgacttga ggcatggtta cgtgcgattc gtccgccacg atcaaaaaat cttcaggaaa
    34441 gaaatccaat agcgtataag gcggttcacc ttctttacgt ccgtccatat gccgagaata
    34501 attttcaatt cccgacgtgt agcccatttc taacatcatt tcaatatcgt aattggttcg
    34561 ctgttctaaa cgttgagctt ccagaagttt gttttcattt cgcaagacag ttaaacgctg
    34621 ttctaattcc gctttgatat tcgctacggc atgctccata tgttcatcat tcgtcacaaa
    34681 gtgagtcgcc gggaagattg ccacgtgttc cgtttctcct aaaacttcac ctgtcagtgc
    34741 attgacttct cggatacgtt cgacctcatc tccaaaaaac tccacacgta aggcacgttc
    34801 atctcttgaa gccggaaaaa tctccacgac atcaccgcgc actcgaaaac gaccgcgctg
    34861 aaaatctata tcatttcttt cgaattggat actgactaag tcacgaatca actgattgcg
    34921 atctagttct gctccctgtc gaatcgaaac tacttgtttt tggtactcaa aaggcgaacc
    34981 taatccaaaa atacatgaaa cggatgcaat gacgatcaca tcatttcttt ctaataatga
    35041 actagtagcc gaatgtcgta atttgtcgat ctcatcattt acacttgaat ctttttcgat
    35101 atacgtatcg cttgaaggca cataagcttc aggctgataa taatcataat aagaaacaaa
    35161 atattcaaca gcattgttag ggaaaaattc cttgaattcc ccgtacagct gaccagccaa
    35221 tgttttgtta tgggcaataa ttagtgttgg tttattcact ttctcaataa gattggaaat
    35281 cgtatatgtt ttccctgttc cagtcgcacc taataagatt tgtgcttttt tcccgccgac
    35341 tacgccatcc actaattggt taatcgcttc aggctgatca ccagccggtt ggtatttcga
    35401 gacaagctca aagtgtcggg aggtatctct ctcaatcatt cttcgtcctc cttttcattc
    35461 attccttttc attttacgac caataaaaag aaaaggaaag caaaggcatt ccttactaaa
    35521 aaattatacc acaccgaaca tactttcgcc acttttttat cttattatgc atgttacgtt
    35581 ttataacata aaatgttaga aactcgcatt tttttcattt tttcgagctt acagtcttgt
    35641 ctgattcagc acttttcgcc tataataata aaatattgga aatttaggag gttgattatg
    35701 aaaagaaagc atttatttca ctcatttgtt ttcttaatga caggacttgt gacacttgcg
    35761 tttggacatg ttgcacaagc tgacaacact acaacggcag agtcaagcac aagcgagaca
    35821 atgcaaaaaa tagagcccaa aaaagacgta tacgtaattg ccagtgactc tgcttttgct
    35881 ccatttgaat ttcaaaacgc aaagggcgag tacgaaggca tcgatgtaaa attgatggaa
    35941 aacatcgcca aactgcaagg atttaacatc cgaatggact accgtggttt ctctgctgct
    36001 ttgcaagctt tggaatcagg acaagcagac ggaatgattg ccggaatgac tgtaacagat
    36061 gaacgaaaga aaagctatga tttttctgat tcttactttg atagcgggat ccaattagct
    36121 gtcaaaaaag gagatacttc gatcaagtct tatgaagatt tgaaaggaaa aacagtcgga
    36181 gcaaaagttg gtaccgaaag tgctgaattt ttaagcgacc atgaatctga atatggctat
    36241 tctatcaaat attttgatgc tgctgatcaa ttgtacgacg ggttaaaagt tggcgcaatc
    36301 gatgcaatga tggatgatta tccggtaatc ggttatgcga tcaaacaagg acaagaattg
    36361 gaaacaccga tcaaaaaaga atctggcgga aaatatgcat ttgccgttaa aaaaggacaa
    36421 aatcctgagc ttcttaatat gttcaatgaa ggattgaaag aattaaaaag aacaggcgaa
    36481 tacgataaga ttattaaaga atatgtagcg gatggttcag aaacttccag tgagtcttcc
    36541 actgctgatg aatcaacatt tcttggactt atccaaaaca actatcaagc attgttgaaa
    36601 ggactttgga gaacaattct cctaactttg atctcctttg ctttggctat agtaatcggt
    36661 gtgatttttg gattattcag tgtagcacca atacgcgctc ttcgcacaat tgctagtatt
    36721 tatgtcgata tcatccgagg aattccaatg atggttcttg cgttctttat tttctttggg
    36781 ttgcctggta tcgttggatt tacgattcct gacttccttg ctggtgttat caccttgact
    36841 cttaacgcaa gtgcttatat cgcagagatt gttcgaggcg ggatcaacgc cgttcctgtc
    36901 ggacaaatgg aagcttccag aagtttaggt ctttcttata atcggacaat gcaaaaaatc
    36961 attttgccgc aagcgatacg catcatgatc ccatcattcg tcaatcagtt cgttatctca
    37021 ttaaaagaca caacgatcat ttccgcaatc ggtgtagtcg aattgctgca aactggtaag
    37081 atcattgttg cacgtacaac acaaagtacg tatgtttact tgatcattgc gatcatgtac
    37141 ttgattttga tcacagcatt gacaaaatta gcaaaagttt tagaaaagaa ggtgaagtaa
    37201 gatggcagaa aaaatcttag ttgagcattt agtcaaaaaa tacggcgata acacagtatt
    37261 aaacgacatc aatgtgtcca tcaacgaagg agatgtcgtc tgtatcattg gcccttcagg
    37321 ttcaggaaaa agtacatttt tgcgttgctt gaatcgttta gaagaacctt ctgacggtga
    37381 aattattatt gacggcgccc acttgatgga taaaagcaca gatatcaact tggtgagaca
    37441 acatatcgga atggtattcc agcactttaa cctattccct catctttcta ttttggaaaa
    37501 catcatttta gcaccgatgg acctgaagaa agaatctaga gaagaagcag agaaaaaagc
    37561 aatcaaattg ctgcagacag ttggtctaga agataaaaaa gatatgtatc ctgaaatgct
    37621 ttccggtgga caaaagcagc gtgtggctat tgctcgtgct ctagcgatga accctgacat
    37681 catgcttttt gatgagccta catctgcact cgaccccgaa atggtcggtg atgtcttgaa
    37741 cgtcatgaaa aagttagcag aacaaggtat gacgatggtt atcgttacgc acgaaatggg
    37801 cttcgctaaa gaagtggcca atcgtgtcat gttcattgac gacggaaact ttttagaaga
    37861 cggaacgcct caacaaatct ttgaaaatcc gcaaaatgaa cggacaaaag atttcttgaa
    37921 taaagtatta aatatttaat aaaaaccaaa aatgcccggt cgcttcatga cgaccgggca
    37981 tttttagttt cttgtttttt cacttaactg taccagccgc atcgcttgtt ctcctgcaca
    38041 aacaagcaat cgctctacct gttccatcgc ataagaaagt accatcggtt cattgacgat
    38101 atcaagtaca tagtcgatac caccttcaga aactatgatg attatcttat tctaaagctg
    38161 gcttttttgt tggtacaaac atgacaacta caaaaaaacc aggcgaacgt atgattgttc
    38221 gtctggtttt taattattaa tgacttagcg tttttactag aaatagaaaa tttgggcaag
    38281 tctactttat atttttacta gaaaaactac ttccgctttt tcccgccaaa agcatcttta
    38341 aacttatcga agaaaccgtc ttcttgctgt tcttcaactt tgatgccgcc agcttcagcg
    38401 aatgcacgta atgcttctct ttgttgttca tttagatttt tcggtgtgat cagttttact
    38461 ttgacatgtt ggtcgcctgt tgcaccacca cgtaacttcg gagcgccttt tccacgtaaa
    38521 cggaagttcg tacctgtctg tgttccagca gggatcttca atttcacatc tccatggaca
    38581 gttggtactt ggacttcatc ccccaaagct gcttgtacaa aacttaaagg caattcatag
    38641 taaatttcag aaccttcacg atcaaagata tcactttctt ctacacgaaa aaccacatac
    38701 aaatcaccaa atggtccgcc gttttctcca gcttctcctt gaccagctaa acgcatttgt
    38761 tgtccttctt ctacaccagc tggtacattc actttaactg aatgtgcttt cttctcatgt
    38821 cctgagccat gacaggttgg acaaggttct ttgatttctt tccctgtgcc atgacaaaca
    38881 tcacaagttt gacggctcat cactcgtcca agtggcgttt ggcgttctac attgatcgta
    38941 cctgcaccat gacatttatg acaagtagtt ggttgtgtgc ctggttttgc accgtttcca
    39001 ccacaagtat gacagatttc ttcccgatta tacttgattt ctttttctac accaaaaatc
    39061 gcttcttcaa aagacaagtc gaccgtatat tgcaaatcag caccttgacg aggcgcattt
    39121 ggatcgactg accgaccgcc tcctccaaag aaagagtcga agatatcttc aaaaccaccg
    39181 aagccgccac cagagaagcc gccgaatcca ccaaatccgc caccagctcc accagcaccg
    39241 taatttggat ctgtgcctgc atgcccgtat tggtcgtatg cagcgcgttt ttgcgggtca
    39301 cttaaaatct catatgcttc tgatacttct ttgaattttt cttccgcatc aggttcttta
    39361 ttgatgtcag gatggtattt tttcgaaagc ttacgataag ctttctttat ttcatcttct
    39421 gatgctcctt ttgagagccc taagacttca taataatcac gttttgttgc cataggcttt
    39481 cctcctattg gttctcatca ttttcacgtc gcttgttatc atatcatatt ttgtttgaaa
    39541 caaaaaccga tacttcaaac tatttttcaa aagaaaaagc caaagtagca actttggcct
    39601 tctctcgaaa ttcagtgaac tgattattta tcgtcgccat cgacttcttc aaaatcagca
    39661 tcaacgacat catctgctcc gccttgagct gcttctggat tttcttgagc ttgttgttgt
    39721 gcagcttgtt cgtatagttt aacagtcaag ttttgaacga tttcgttcaa tgcatcgcgt
    39781 ttagttttca tttcctcgat gttgtttgct tcaacagcgg cttttaattc gtcacgagct
    39841 gtttctgctt ttttcacttc ttcttcgtcc actttgcctt ctaattcttt caatgttttg
    39901 tccacagaga atagcaatgc gtctacatca ttacgtaggt caacttcctc tttacgagct
    39961 ttatctgctt cagcgtttgc ttctgcatct ttcaccatac gttcgatttc ttcgtctgtt
    40021 agacctgaag aagatttgat cgtgattttt tgttcttttt gtgtacctag atctttcgca
    40081 gatacattta caatcccgtt tttatcaata tcgaatgtta cttcgatttg tggaatacca
    40141 cgaggagctg ctggaatatc tgtcaattgg aatcttccta gagttttgtt gtcagcagcc
    40201 attgggcgtt caccttgtaa tacatggatg tctactgctg gttgattatc tgccgcagtt
    40261 gagaatactt gtgatttact tgttggaatc gttgtattgc ggtcaatcaa tttagtgaac
    40321 actccaccca ttgtttcgat acctaatgac aatggtgtaa cgtcaagtaa aacaacatct
    40381 ttcacatcac cagtgatcac gccaccttgg atagcagcac ccatagcaac tacttcatca
    40441 gggttaactg atttgtttgg ttcttttcct gtttctttac gtactgcttc tacaactgca
    40501 gggatacgag tagatccacc gactaaaata acttcgtcga tttctgattg tgacagacct
    40561 gcatctttta acgcttgacg tactggtact tttgtacgtt ctactaagtc agctgttaat
    40621 tcatcaaatt ttgcacgtgt caatgtcatt tccaaatgca atggtccaga ttctcctgct
    40681 gtgataaatg gcaagctgat ttgtgtgctt gatacgccag ataaatcttt tttcgctttt
    40741 tcagcagcgt ctttcaagcg ttgtaaagcc attttgtctt gagacaagtc aatgctgttt
    40801 tcttttttga attctgctac catgtagtcg atgattttgt tatcaaagtc gtccccacct
    40861 agatggttgt cgccagctgt tgacaataca tcaaagacac cgtctcctag ttcaaggata
    40921 gatacgtcaa aagtaccgcc accaaggtcg aatactaaga ttttttcgtc gcgatctgtt
    40981 ttatctaaac cataggcaag agcagctgca gttggttcat tgacgatacg ttctacttct
    41041 agaccagcga tcttaccagc atcttttgtt gcttgacgtt gtgcatcatt aaagtaagct
    41101 ggcacagtga taactgcttt ttcgactttt tcacctaaat agtcttctgc gaatcctttg
    41161 atatattgaa ggatcattgc tgagatttct tgtggtgtat atgatttacc ttctacatct
    41221 actttgtaac ctgcttcacc gatatggcgt ttgattgatg aaatcgtatt tgggtttgtc
    41281 acagcttgac gttttgcaac ttcccctact tggatttctc catttttgaa tgatacaaca
    41341 gaaggtgttg tacggtttcc ttctgggtta gcgatgattt ttgcttcgtt tccttccaat
    41401 actgctactg cagagttagt cgtacctaag tcaataccga taattttact cataattaac
    41461 gtctccttct ttaattgaaa gtgtaagttt tatatccgca caattattgt gcaacaatga
    41521 ccatagttgg tcttaacacg cgatcatgta atttgtagcc ttcttgtagt acttctacaa
    41581 tcgtatcggc tggtgtatct tcagtcgctg gaacagtttg tactgcttga tgaagattag
    41641 gatcaaaggc ttctccttta gcagggattt tttcaattcc ttcttcttcc agtgcgttac
    41701 gcaaactttc caagaccatt tccacgcctt ttttcaaacc tgccccttga tcatctgaca
    41761 cttctgtggc taatgcacgt tccaggttgt caattgatgg aagtaatttt ttggctaaat
    41821 cttgtgaacg gtatttttgt aattgctcac gctcattttt tcctcgattt gccatatttg
    41881 cgatttcagc acgagcccgc aagtatttat cttccatttc ttctaactca gcttttaatt
    41941 tcgctgtttc gaattcttcc gcttcagctt ctgaaatacc cgaatcttct gcagcttcga
    42001 catcgatttc cggttctggc tgagcatctg tcatttcttt atctagttct tcttgatttt
    42061 ctttcacgat acagtcttcc tttcattatt cagtcagctg tttgattgct cttactaaga
    42121 aagagagaac gatcagctgc aaatagcttc gttattcaca ttacgagttt gacaagtcaa
    42181 gcgagcggta gtaatcagca agtttagctg ctagttctct tcgataaaca tcgactagac
    42241 cgaacatccg agaataaggc atactagtag gccctaacaa ggctattgta cctcttccat
    42301 gaccatctat ctcgtaactt gcttgaatca tactcatatt ctgcaataaa tcattgccta
    42361 attcagcacc aatcttgata tgaatattgc tatccatcgg agtgatcatt tgtgtaagtc
    42421 cgtctgagtc tttcataaaa gaaaacatcg atttgaactg atcaagatct tgacggggct
    42481 cataatccaa gagattcatt tgtccactga cataaacctt ttcttcaaaa acatgaccaa
    42541 gaacggtgtc aaataactga gacatccctt ctgtcgtctg gaaatattta tgaagaatca
    42601 tcggaatctc cgtacgaagt ttatggtaca ccgtcatcag aggatctccg actagacggt
    42661 cgtttacaat ctgtaccatt ttttctagtt cctgacttcc catatattcg ggaagagtga
    42721 acacttggct ttctacattc cccatatctg ttacgacgat agcaataatc tgtcgattat
    42781 tcaagggtac gatacgaaag ccagtcagtc ggcggtcttt tatttcaggt cccaatgaaa
    42841 aagtcgtata gcttgtcagc tcagataata tctcagcaga ttgcttaata atctcattga
    42901 tttcatgaaa ttctctgcct agagactgac gaatcagcag caaatcatct tctgctacgc
    42961 gagcaggttt taacaaatga tccacgtaat aacgatagcc taaagcagat ggaatccgcc
    43021 cagaagaaga gtgagtcttc aacagcaatc cttcatcttc taatgctttc atatcattgc
    43081 gaatcgttgc tgagctggcc ttgactccct cagacattaa tgttttggaa ccaacgggca
    43141 cgccactact cgtatagtgt tggatgatca gacgtaaaat gtcgttttgt cgctttgtta
    43201 acatcttcct cacctctcat tagcactcat atattctaag tgctaattca cttataaata
    43261 tagcaagaag cccttctcct gtcaagagaa agactccatt ttttagcact ctaaacacaa
    43321 gagtgctaat tctaaataat tgatgtaaat ccagttatat caatacttca atacctcatt
    43381 gtcaaacttt ctaaactttt tcttttcaag tttcgataaa aaacaaatat tcttcaaact
    43441 atttttcaaa taaaaagcgc tcaaacacat tgttccccaa aaaagtccct cttggtgtca
    43501 aataaacacg gtcttcttca ttaacaagta acccttcttg ctctaattcc cttagcacat
    43561 caccgtacac cgattcaatt gttttttgat agcgatcata gaaatgtttt ttagtaatcc
    43621 ctgttttttt tcttagtcct aagaacattt cttcttctat ttgatttttt cgtgtcaatt
    43681 tttcttcttc caataccggg cgttcatttg ctcgtaatgg gcgtaaatag tgttggattg
    43741 gtcctttgtt gcggtaacga atatctccaa gataaccgct agcaccagct ccaaacccaa
    43801 aatagtggtc attgttccag tacatgaggt tatgttgaga ttcatgtccg gagagcccaa
    43861 agttacttat ttcatattgg tgacgtccat gcttttccat tgcttcaatc gtctcttcaa
    43921 acattctggt ttccgtttcc tgatcaggca gttcgagtct tccttgtcgt acccagttca
    43981 tgaacattgt tttgttttcg aggatcaatg agtacaaaga atagtgaggc agatccaaag
    44041 ccaaagcttt ttccaaagta tcccgatttc cttctagtgt ttgtcccggt aatgcataaa
    44101 tcaaatcgat gcttacattg gtaaaatttt ctgcttctaa aaattgcatt gtttgataca
    44161 catcttctgc agtatgttta cgtccaattt ttttcaataa gcgattgtca aatgtctgca
    44221 ctcccatgga taaacgattg acgccatagt ttttcatcac ttgaagtttt tcacgggtca
    44281 aatcaccagg attcgcttct acagtaaact cattaccatt ttgaaaaggc aggatctctc
    44341 tagcacctga taatagtcga tcaagttgtt tagcagataa agaagttggt gttccgcctc
    44401 ctacataaag tgtttccaaa ttcgctgtag gatgactttc cagcattaat ctcatttcat
    44461 tcagcagcat ttccacatac tcatctactg gctgcccttc taaaaaaact ttattaaagt
    44521 cacagtaata gcagatatgc tcgcaaaatg ggatatgaat gtacgcagaa accttgtttt
    44581 ctgcgggatt tattgtttct atcattgaaa ttctttctcc ttacgtcgaa aagtcgtgcc
    44641 tgttttcgac cttcttaaaa tcgcgttcaa gcaccaactc attgaaataa gccgcatatt
    44701 tagaatcaag gaaacctagc tctaaaaatg cttgcttatt gtcatcggag ctacacggtg
    44761 cttttacagc ctctcgataa acggtaatcg caaagctgac cttcggcatt aagtcaaaat
    44821 ctgcaaaaat atgaaaagct attttcacag attcctcctt aagtgctcag accacaacgc
    44881 tttgcttaca acctctcata tacgcatact aaacgtattt ggcgaacaaa tccactaaaa
    44941 aatccaagaa tcgacttctt ggatgatttt tttacactca tgattcttcc taaacaattt
    45001 tgtccttatt ttattcataa aaaaatctag tcaaatctct cacttcaaga gttttaacta
    45061 gattccaatt attatttttt tgtaaaaata aacagtctct taatcataga catttcttcg
    45121 gtgccctact tgaatagcga taacaacgaa ttgattatct tctatctcta ccaatacacg
    45181 tatttgccaa tcagagctgc ttctgaattt cgggcgtgaa ttttaaacgg caggtcattc
    45241 cagcccacca aattcattaa ggattcttcc atgaatactg ttgttttccc acttcttatc
    45301 caatgtttat gggcaatttc agccgtttga cgattgtatt catcttctag ttgttccatc
    45361 gaatagtttt ttagaagttc agataaagta atcccataaa attcagccat ataagaaagc
    45421 cattcttttt tggcgtcact tacacgaacc gtaatcatag ccctataatt gcctctctta
    45481 aagaatctca tgcatttcct gatgtgattt tagaaaaatt tacttgctaa aaaactgtga
    45541 aaatgtagga gctagcatcc ttgtagtaaa tgatcgagct ctttattata cattaaaaaa
    45601 gttcagaaat tttgatgaag tagccaacta ctttttcatg agtaaagcaa cactagaaaa
    45661 agaatcctcc agtacttaga atttactgga ggatgtactt ttgaagaagc ctataggtta
    45721 tttaaaaaat attactacag gaaggaacga gaattacttc gttacttata atagaactta
    45781 gtcttatata gaaggcatag ctcacattag cttatttgtc ctataaaaat aaattacggt
    45841 attttagaaa agctattttc tcttttttca acgatcttca gcaatttttc tttatcgtca
    45901 ggtaactcaa gttcatgaat tctagcatga caattgggac ataacgcgat acagttttca
    45961 atcacatctt ttccaccttg cgacaaatat tctatatgat gacaatgtaa ataaggtaca
    46021 ccgtctttta taaatggccc tttttcttgg caaagttgac aaataccttt tgcaagtcgt
    46081 ttaacatatt cagatacgaa gggattacgt ttatatattt ttgtttttga gaagcaaaac
    46141 tcctcttcgt taacatattt tgcagccatt tttttattat aattactaac ttttcttgct
    46201 tcttcctcta atttagcatt agataaatta cgaatatgtc tagtttgatt ttcttcctgg
    46261 ttatatatct ttatcttcaa tcaattgctc ttgatttatt aaagctaatg gaaatttata
    46321 tacttttctg ctgttgcccg aactatcttt ttcattaaca gtatatattt gtcctgctaa
    46381 atatacttct cccagatatg tgtgttcttt tggtttaaat gtttcaaata aatgaatctt
    46441 tacaaaatta ctttttgaat tggctaaaac tttattttga ttcttatcca atacttgatc
    46501 cccttttagc cccataccgg tatattgtaa aataccattt aaccacttat ctttataaac
    46561 attacttcct gtttgatcac taaacagtac aagagtatta gtttgatgag atcttctcat
    46621 tccaccctgt ttggaacatt taaacttctc tgaaatttct ttgttagtat atactctacc
    46681 aacttctaac tcacctaata tgtttttaga ccaattttct cgccatgtaa taatcttatc
    46741 aagattaaac atttctccct cttttggcat gccatttcta attaaattag ctaatacacc
    46801 agtagaaata tctaattttt ttagtaattc tttcttagta atcaatgttg gattcataac
    46861 aatgcagctc ctaatgatat caatataatt gcattagata acttgaaaaa tcgaaaaaaa
    46921 ctcattttgt gtcaagttat ctgtaatata taaaaatatc attattatac ctcatctatg
    46981 tgaattttta ctattcttac cttagcatat tctttatagg tatatcagag ctaatcaaac
    47041 taaaaaatac ccgaagtctt cttatgtaaa aacaccggtt attacttaaa tatgacttag
    47101 gtaaaacaat gagtgatcca attataaaat tttatggcga ataatttagt aagcgctaca
    47161 tattgaatga tttagaaatt ttgaaaaagt agctagttcc ttttatacca gaaaagtaaa
    47221 cctaataaaa aacacaacta atttttagct gtgtttttta agttacaata attatctatt
    47281 taatagtctt aattcattaa aataaaaaac tcaactttac aaggatatgg tttaaaaaat
    47341 ttatttatcc aacccaacta gttttcgctg cttacgattt gcttcatctg ctacgtaatt
    47401 cattactgtt ttaaattctt tcgctgaggc gttttctgat aaatcagcga tacttttatt
    47461 atacttcttc acgatttgtt gtacttgttc tttctcagtc atgtaattcc tcctccaact
    47521 cttttgaagt atattgtatt ggtagtacta attgaatatt atctgctgca taatgcactt
    47581 cagaaatgtc gttttgataa tccttaatca ttatgtgaag gactacttca ggatgtctat
    47641 ttttgacttt ttcaatattc tctctggact taaaaaacgc attgataaac atttcttttg
    47701 gcacatgacg tccttcagct agttcacgtt ttcgagtgaa atcccaggca actttgggat
    47761 cttggtaaac atagaaaatc gtaacgcgaa agttattttt aagggctcga ataatatttt
    47821 tctctgcact aaggatagca aaagtatcct ctagtatgaa agaatagcca tgttccagta
    47881 catattttaa tgattgttca actaaccaag aagaagcttt ctgaaaaaca cttgagttct
    47941 ttccattgta ttctggaaat tgtgctctaa aatagtccgc atcaatcaca acatggttgt
    48001 cataatttta agctaaacct agtgcaacct ctgttttacc tgctcctggg cttccagcca
    48061 tgaataccgc atctttttca tcatcaggga tttttccctt gatgattgtt tcaataaatt
    48121 tatctttatt gaccctagcg taatctaaat acttgtcttt aggttccaag attgcacctt
    48181 ctttccttct tctatattca tactaattat acgatattta tataaaaaat ataacggtca
    48241 ttttaaccat attaacattt agtcacagta atagcagata tgctcgcaaa atgagatatg
    48301 aatgtacgca gaatccttgt tttctacggc attcgctttt ttttcattgg actagttata
    48361 tatttctagg gttgttttca tgtctgagtg cccgatatga tttatttttc gtttctataa
    48421 ttactttttg ccttccagtt tcttttaaga ttgtactcaa gcaagtggga attggttctt
    48481 ttgtcatcca tgtatgcaaa atgttataat gctggtaaac aaaaatgatt acctgtcatt
    48541 tttatttagt tcgtatttaa ttaagttgac tgtctgtata ctctgtctaa aacttaagaa
    48601 ttgttgtttt cgctgtcgct atccaaattt aatgaatgtc tgtatgttaa aactgttgtc
    48661 cttgtcatag atagtaaagg agagcttgaa aatgaaaact gcgattacgt ttctattaga
    48721 ggaatatgcc gattgggaag gtgcttatat tactagcaca ctaaaccaaa aagagggatg
    48781 gcaaaacaaa actgcttcta tccaatcaga ggtcacttcc attggaggat taaaaacaaa
    48841 aatcgattac ttaatcgaac aaatacctga aaaagttgac ctttttatta tgatcggtgg
    48901 caattcatgg gacataaaaa atcatcattt atatcaaata atcgataaat tgttaaaaac
    48961 agacgtacca gtagctgcta tttgtggggc agttgattat ttagctcaga gcggattatt
    49021 gcaaggttac aaacacaccg gaaatgcgca atttccttgg ttagaatcgg aaaattatca
    49081 gaataaagaa gattttatag aacaacaagc tgtgaaagat cgtaatttga ttactgcgaa
    49141 tggaacagct cctatagaat tcacagactt gattcttcaa gcagtccatt ttgaaacaga
    49201 agaaataaga gaaaaaaaga ttcgtctata caaacttgga ttctatgatt actataaaaa
    49261 gtacggagat cctttctcct aatcattctc ttcaattaaa tactccttat caatagtatg
    49321 acaagtaatt ttactgatgg ttatttgtca cactttttct gacatttatc aaagaagata
    49381 cactctattt atattcatag atggaatggc aacagttttt ttgacaaatt ttataaggtg
    49441 cagaacttct ttccgtatgc tattccgatt gtccttgaca atgagcctcc tcggatgtga
    49501 cgatcttcgg caggagctaa cagttaaagt tagactgcct cgctagcgtt agcacatccg
    49561 agctcattat caaggattcg ctgcgccaat ctct
//
LOCUS       DACDRN010000012        47473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDRN010000012 DACDRN010000000
VERSION     DACDRN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.012"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(56..790)
                     /locus_tag="IWJ80_000917"
     CDS             complement(56..790)
                     /locus_tag="IWJ80_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9460670.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1117..1527
                     /locus_tag="IWJ80_000918"
     CDS             1117..1527
                     /locus_tag="IWJ80_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP9460671.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1520..2221
                     /locus_tag="IWJ80_000919"
     CDS             1520..2221
                     /locus_tag="IWJ80_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP9460672.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2221..3807
                     /locus_tag="IWJ80_000920"
     CDS             2221..3807
                     /locus_tag="IWJ80_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9460673.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3826..4047
                     /locus_tag="IWJ80_000921"
     CDS             3826..4047
                     /locus_tag="IWJ80_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP9460674.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4044..4805
                     /locus_tag="IWJ80_000922"
     CDS             4044..4805
                     /locus_tag="IWJ80_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP9460675.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5185..5694
                     /locus_tag="IWJ80_000923"
     CDS             5185..5694
                     /locus_tag="IWJ80_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9460676.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5769..6308)
                     /locus_tag="IWJ80_000924"
     CDS             complement(5769..6308)
                     /locus_tag="IWJ80_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP9460677.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="IWJ80_000925"
     CDS             complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="IWJ80_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP9460678.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7375..8172
                     /locus_tag="IWJ80_000926"
     CDS             7375..8172
                     /locus_tag="IWJ80_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP9460679.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8200..8835
                     /locus_tag="IWJ80_000927"
     CDS             8200..8835
                     /locus_tag="IWJ80_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP9460680.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8855..9628
                     /locus_tag="IWJ80_000928"
     CDS             8855..9628
                     /locus_tag="IWJ80_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9460681.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10036..10833
                     /locus_tag="IWJ80_000929"
     CDS             10036..10833
                     /locus_tag="IWJ80_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP9460682.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10811..11224
                     /locus_tag="IWJ80_000930"
     CDS             10811..11224
                     /locus_tag="IWJ80_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460683.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11208..13853
                     /gene="mutS"
                     /locus_tag="IWJ80_000931"
     CDS             11208..13853
                     /gene="mutS"
                     /locus_tag="IWJ80_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9460684.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13871..15979
                     /gene="mutL"
                     /locus_tag="IWJ80_000932"
     CDS             13871..15979
                     /gene="mutL"
                     /locus_tag="IWJ80_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP9460685.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16001..16561
                     /locus_tag="IWJ80_000933"
     CDS             16001..16561
                     /locus_tag="IWJ80_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP9460686.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16646..18634)
                     /gene="treP"
                     /locus_tag="IWJ80_000934"
     CDS             complement(16646..18634)
                     /gene="treP"
                     /locus_tag="IWJ80_000934"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP9460687.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18857..19570
                     /gene="treR"
                     /locus_tag="IWJ80_000935"
     CDS             18857..19570
                     /gene="treR"
                     /locus_tag="IWJ80_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP9460688.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19647..20093
                     /gene="msrB"
                     /locus_tag="IWJ80_000936"
     CDS             19647..20093
                     /gene="msrB"
                     /locus_tag="IWJ80_000936"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP9460689.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20263..20865
                     /gene="ruvA"
                     /locus_tag="IWJ80_000937"
     CDS             20263..20865
                     /gene="ruvA"
                     /locus_tag="IWJ80_000937"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP9460690.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20878..21879
                     /gene="ruvB"
                     /locus_tag="IWJ80_000938"
     CDS             20878..21879
                     /gene="ruvB"
                     /locus_tag="IWJ80_000938"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP9460691.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21908..22486
                     /locus_tag="IWJ80_000939"
     CDS             21908..22486
                     /locus_tag="IWJ80_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9460692.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22538..23020
                     /locus_tag="IWJ80_000940"
     CDS             22538..23020
                     /locus_tag="IWJ80_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP9460693.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23137..23511
                     /locus_tag="IWJ80_000941"
     CDS             23137..23511
                     /locus_tag="IWJ80_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460694.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24189..25541)
                     /gene="brnQ"
                     /locus_tag="IWJ80_000942"
     CDS             complement(24189..25541)
                     /gene="brnQ"
                     /locus_tag="IWJ80_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9460695.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25688..26278
                     /locus_tag="IWJ80_000943"
     CDS             25688..26278
                     /locus_tag="IWJ80_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9460696.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26406..27755)
                     /locus_tag="IWJ80_000944"
     CDS             complement(26406..27755)
                     /locus_tag="IWJ80_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9460697.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27923..28663)
                     /locus_tag="IWJ80_000945"
     CDS             complement(27923..28663)
                     /locus_tag="IWJ80_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9460698.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28676..30268)
                     /locus_tag="IWJ80_000946"
     CDS             complement(28676..30268)
                     /locus_tag="IWJ80_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9460699.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(30836..31132)
                     /locus_tag="IWJ80_000947"
     CDS             complement(30836..31132)
                     /locus_tag="IWJ80_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460700.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31308..32033
                     /locus_tag="IWJ80_000948"
     CDS             31308..32033
                     /locus_tag="IWJ80_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP9460701.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32026..32868
                     /locus_tag="IWJ80_000949"
     CDS             32026..32868
                     /locus_tag="IWJ80_000949"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP9460702.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32871..33392
                     /locus_tag="IWJ80_000950"
     CDS             32871..33392
                     /locus_tag="IWJ80_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP9460703.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33645..34208)
                     /locus_tag="IWJ80_000951"
     CDS             complement(33645..34208)
                     /locus_tag="IWJ80_000951"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9460704.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34459..34728
                     /gene="rpsO"
                     /locus_tag="IWJ80_000952"
     CDS             34459..34728
                     /gene="rpsO"
                     /locus_tag="IWJ80_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP9460705.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34916..37042
                     /gene="pnp"
                     /locus_tag="IWJ80_000953"
     CDS             34916..37042
                     /gene="pnp"
                     /locus_tag="IWJ80_000953"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP9460706.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            37552..37818
                     /locus_tag="IWJ80_000954"
     CDS             37552..37818
                     /locus_tag="IWJ80_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9460707.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            37962..39953
                     /locus_tag="IWJ80_000955"
     CDS             37962..39953
                     /locus_tag="IWJ80_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP9460708.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            40307..41608
                     /locus_tag="IWJ80_000956"
     CDS             40307..41608
                     /locus_tag="IWJ80_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9460709.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(41738..43108)
                     /locus_tag="IWJ80_000957"
     CDS             complement(41738..43108)
                     /locus_tag="IWJ80_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP9460710.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVNPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(43290..43613)
                     /locus_tag="IWJ80_000958"
     CDS             complement(43290..43613)
                     /locus_tag="IWJ80_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9460711.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(43938..44546)
                     /locus_tag="IWJ80_000959"
     CDS             complement(43938..44546)
                     /locus_tag="IWJ80_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP9460712.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            44876..45592
                     /locus_tag="IWJ80_000960"
     CDS             44876..45592
                     /locus_tag="IWJ80_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP9460713.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            45605..46300
                     /locus_tag="IWJ80_000961"
     CDS             45605..46300
                     /locus_tag="IWJ80_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP9460714.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            46384..47013
                     /gene="udk"
                     /locus_tag="IWJ80_000962"
     CDS             46384..47013
                     /gene="udk"
                     /locus_tag="IWJ80_000962"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP9460715.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        15392 a         8788 c         9878 g        13415 t
ORIGIN      
        1 aataaaaaaa cttggtacgc aatcgctgta ccaagttttt ttatttagct tttgattaag
       61 ccataaataa taatttatat aagaatactg ctacgacacc agcacagata ggtgctacaa
      121 ctggtaccca tgcatatccc cattgagaag aacctttatg tttcaatgga agaagtgcgt
      181 gtacaagacg tggagcaaag tcgcgggctg ggtttagtcc aggacctgta ggaccaccaa
      241 gtgaagcaac taatgtccat acaaggaaac caagtgctaa gtgagctgta ccaagattat
      301 cttggaagaa tggtgctttt gtaataccta gggcaccaaa gaataaaacg aatgaaccaa
      361 agaattcatt gacgaaacca ttgaatttag aaccaacgct gttgattgtt gagaatgtac
      421 ctaacacatg gtttacgtct tctgtttggt cgtagtaagg tttgtaacaa gctactacga
      481 tcaattgtcc aatcgctgca ccaatcaatt gtgcaatgat ataaggaaca acttcagccc
      541 aagggaatag accattagat gctaaagcaa ttgtaaatgc tgggttgatg tggttaccgc
      601 tgattccacc aaaaatcatt gctggcatca taaccccgaa tccatacccg acagcaatca
      661 gcatccagtc actgccatat ccttttgtac cttttaaatc tacgtttgct acagcaccgt
      721 tacctagaac gattagtaat gctgtaccaa tcatttccgc cgcaagacgg gtcattagtg
      781 agtaatccat aaatacctcc aaaagttata aaaatgcaaa aaaaaaagaa acatcaacac
      841 gaaaagcatc ggtgcagtgt ttcgtgtcgg aaggatttct gcagtcttaa caaatgtgaa
      901 taaaaaaatg aattccatta cttataagaa accagaccaa cagtaaaact ccaactcttt
      961 ttaaaagttc tccacaaatc accaagctta actataaata taccaaaaaa aaaacgtttt
     1021 ttttctcaaa aattgtatct ttgaaacagc atgttttgaa actttcagaa aaggtttgtt
     1081 agtaaaatga aagctatacg taaggagtgt tgtttgatga aattttttca agagtttttc
     1141 attattattt tcttttctgt attaggagaa ggcatacgga tttttgctca tttacctatt
     1201 cctggaagta tcttaggtat tcttttttta tttttagcat ttgaaatgca tcttgttgat
     1261 cctgaaaaaa tcggagcaac tggtgatttt ttattgaata atctagccat tttatttgtt
     1321 cctgctggtg taggtttatt ggaatatttt gatgatattg cttcgatatg gcctgtttta
     1381 ctgggagcag tcgtcgtgtg ttccatcgtc acgatggttg cagcgggaaa aacggcagaa
     1441 ggtgtagaag ctttgctggg ctatgtccgt aagaaaaaag cagaaagggt atctgtaaac
     1501 gaaatggagg agaatcaaat tgactgagtt ttttgtgaat ccattatttg gcttaagttt
     1561 gactttagga gtgtacgtat tatctgacaa atttttgcgg aaatataaac tgcctgtttt
     1621 gaatcctctc gtattatcga ttgttgttat tatcttattt ttgcattttt ctgggatcag
     1681 ctataaagac tataatatcg ggggcaattt tttgactatg ctgattactc ctgcgacagt
     1741 ggcacttgcc atccctcttt ataaaaactt ccgtttactg aaagagaatt tttatcctgt
     1801 aatggctgcg attttagcag gaatagtcgc aaattgcttg gttagtgtag ggttcggtta
     1861 cttcttctct cttcataaag agatggtgat ctcattacta cctaaatctg taacgacggc
     1921 tatttctgtc gatttatctc atacgatggg cggggtgaat gccgtgacac ttgctattgt
     1981 tgtctctaca ggtatttttg gttcgctgat cgccactcat attttccgcc tattcaagat
     2041 cgaaagtcca gttgctcgcg gtgtggcgtt aggaagtact tctcatgcta ttggtacagc
     2101 aaaagcaatc gagattggtg aaatagaagg aatcatttcc ggacttgcga tatgcgtcaa
     2161 cggaatattg accgttttat tgctaccgtt attctttcag ccttttgctg gcttgttttg
     2221 atgcgaaaac cactatttta tgctacaata accaaaacgt tcgagcataa aaaaggaggg
     2281 aaagacgtgt atttgtcttc cgagtatctt aaacaatttg ataaagatct atattataag
     2341 attctcctcc tagaaagttt tgaggatcaa gcttggcata ccgctgcaca attagcgcaa
     2401 gttgttcagc tggatgcccg cagcgtgtcg aaatacctga acgagctgtc taaaaactat
     2461 cagcagttta gcggtaagac acaccctttg tttacaaaaa atcaccgttc tggttataac
     2521 ttttatgata cgctcgattc aattgaacat gagcgttttt tgatctatct cgtgcaaagt
     2581 acgctgaaat tccagttgct gcacgacatt ttttttgaag aattccatac aatgtaccaa
     2641 tttgcgcaaa agcattacat tagtgaatct actgctcatc gaaaaatcaa tgaatggaaa
     2701 cagcaactgc aaacttacgg aattcggctg caacgaggta catatatcgc tcaaggcgaa
     2761 gaagaaatca ttcgacttta cttgcatatg acattctggc agttgtttcg agggaaaatc
     2821 tggccatttg aaacgatttc tcaaatggac gtaaagaata tggcagaaca tatcatggct
     2881 ttttttaatg ttcgtctgaa cgagatcaaa aaaagacgat tggaatatat gctaggtgcc
     2941 ttttttttaa gaaaatcaca gaagcattat gttgtattga atgagaaaaa aagacggctg
     3001 atttctgaca atctgttgtt ccagcgattt tgccaggtca tggagccggt atttcctaat
     3061 tattttcaag tagaagacga gttaggcgca ttatttcttg tactgatgac tcgtgaagaa
     3121 tattatagtg atcctaagat acggaagaaa atttttgatt ttcatcaaca agcaaaaaca
     3181 ccgcccttta cagccctttc agaagcaaaa gccgctcttt ctctttatca ggaagagcag
     3241 gggctgccag cagaaaatct aaccttcgag gcagaaaact atttattcag cagtcatttt
     3301 ttcgcctatc tttttcctaa tgcgaaagaa acgattgatg gcaatagtag tgattttatt
     3361 aatcatttag ttattgaaaa caaggaattg aaacaatggc tcgtacattt ttttgaaagt
     3421 agacacaaac atcccaatca tctggcattt aaaaatcatg cgtttttgat ggggcggtat
     3481 ctgactgtgt ttaaaacatt aggagccttt actccacagc ttccaaagat cacgatcctt
     3541 ttaatgacag atttcccatt atttgaagag caactgttag aggagggatt acggacattt
     3601 ttccgtaatg agtatcagct gatttttctg ccaacagatt atcgggggag agaagttgat
     3661 cttctgattt cgacaagtaa agtccaccgc aaaccatggg cggatcttga ctattttatt
     3721 gtgactgaag aactaaaatt aattgattac atccaactat ctcaaaaatt tgaaatgatt
     3781 caaaaacaaa aactattaaa acaataacaa agaaggagaa aaaagatgac atttgaagaa
     3841 attttaccag aattgaaaaa aggtgcaaag attattcgta aaggatggag tggatttgaa
     3901 ctttatgttg ttctcgtatc cgatgatgaa tttgatggct gtccggtgac gccgtactta
     3961 ttgatcaaaa caagtgacga aggtttttcc agcttcgctc ctacagtttg tgatatttta
     4021 gcagatgatt gggcgatcgt agaatgaaaa accagtatcc agatctagct aaacaggttg
     4081 tatttatcac aggagctgct tctggaattg gtgctgcgca agcccgagcg tttcttgaac
     4141 aagatgcttt tgtatttgga ttagatcaaa agttcggtaa aatggaagaa atttctttgc
     4201 gatttcctga aacttttgcc tacgcactag gtgatgttcg acaaatgaaa gaactccaac
     4261 aagcggtatc gaaatgccaa aatcattttg gtgaagttac gattttactg aatacagcgg
     4321 ggatattgga tgcctacaaa cccttgatgg aaactgacga atcgctgtgg gatctgattt
     4381 atgagacgaa tgtgaaaagc atgtatcagc tgacaaaaat catccttcct gatatgatcc
     4441 gccaaaaaag cggaacgatc atcaatatgg cttcgattgc tggcttgatt gctggtggag
     4501 gtgggatcgc ttatacaagc gcgaaacatg cgattgtcgg atttaccaaa caactcgctc
     4561 tggattacgc aggacagggt atccgagtaa aaggaattgc tccaggagcc atccaaacac
     4621 caatgaatgc ggctgatttt gaagggaaag gtgagatggc agagtgggtg gcaaatgaaa
     4681 caccagtcaa gcgatgggca cagccggaag aagtagctga gctgacgtta tttcttgcct
     4741 ctcctcaagc aagctacata caaggaacga tcatcccaat cgacggcgga tggttgttaa
     4801 aataaccttg tgtttttttc aagcttctgt atgataaaac taaagagaga aacaacggtt
     4861 gatgataaaa aaagtcgatg actgttcccg acattcgatg ccgaacagcc agttcaaaaa
     4921 attcttttta ataatcgtta atattcgaaa ctaagcggct tttcacgacc tcttgatata
     4981 aacgatattc aaaagcttgc aactgcggta acaagtccag ccaaatgaaa aatataagga
     5041 gctattttta tttgaccatt cttattactt gttgcagggc agctttttca ctaccttctg
     5101 cgacacttca gttggaattt tcttcccatc ctttggtcgc ggtcattacc aagataaaat
     5161 tatcttgcaa aggagaaata gaacatggaa aaaaataata cccgttcagc caaacgctgg
     5221 acaacgacag atgtgactcg gatggcgcta gtcactggtt tatatgtggc agtgacgctg
     5281 gttttgtctg tctttagctt tggagtgatt cagattcgtc tctcggagat gttcaactat
     5341 ctgtccttgt ataacaaaag atacatcgtc gcggtaacag cgggagtagc tttggctaat
     5401 attgcttctc ctcttggact gatagatgtt gcagtaggaa gcgtcagcac gctgcttgta
     5461 ttgcttatca actataaaat cactagtcgc atcaaaaaca tgaaaatcaa aatggccgtg
     5521 acagcatgcg tatttgcttt ttccatgttt acagtagctg gtcagctaac gattttatat
     5581 caactacctt ttttcttgaa ttggtgggtg attgctttag gagaattggc atcaatgatt
     5641 gtcggtggga taattattta ttggatcggt aaaaaagtgg atttgtcaaa ataaagatct
     5701 gtttaattta aaatagggca gcatggaaga gactgaatgt tgagtcttct tccatgctgc
     5761 ttttttgatt aatttgtatg aagaaatcga tttagccgtt ttttcatcaa gataccgata
     5821 taagctgctc cagttgcctt gattgcttcc ggcagtaaaa acggcagtag tccactggca
     5881 aatgcagttg taaaagtaag atcaccactt attttcagcc agatcgttcc gcagatcaat
     5941 gcagcaagag agcccattag attggcaaag attgcatata agtaatgagc atccgttctt
     6001 tcgatcatcc aaccagttat ccacgtttgt aagagaaatc caatcaaata tccgccagtt
     6061 ggcccaaata aaacagctat acctccagtc atccctgaaa atacaggaag accaatcaag
     6121 ccaagtaaaa gataaactcc aacagatatc aatcctagtt ttcttccgag aaaagttgct
     6181 gttaatccaa tagcaaatgt ttgccctgtc aaaggaacca aaccaaaagg aatggtcatc
     6241 tgagaaagga cagaaataat cgcggcaaat tccgcagcaa gtatcaattc tttcaatgtt
     6301 cggttcataa cgccctcttt tcgttaactt aatattttta tttggttaac gaaattgtaa
     6361 aacaaagtga ttactttgta aatagaaaat ataaaaaaag accagctatc caatagatag
     6421 cggtcttttt caagaaagct tttgaaatta taagctttgg ttgtagtatt caacgacaag
     6481 agcttcgtcg atttctggat atagttcgtc acgttctggt aaacgagtca atgaaccttc
     6541 caatttttca gcgtcaaagc ttacgaatgc tgggcgtcca acagttgctt ctacagcttc
     6601 ttttactgtt acgatgtttt gtgatttttc acgaactgag atcacttgac ccacttctac
     6661 gtggtatgaa gggatatcaa cgcgtttgcc atcaactaaa atgtgaccat ggttaactaa
     6721 ttgacgtgct tgacgacgag tcgttgcaag tcctaaacgg taaacaacat tgtctaaacg
     6781 ttgttctagt aagatcatga agttaacacc atgtttacct tctttgattt tgctagcttt
     6841 tacgaacaag ttacggaatt gacgttcgtt cattccgtac atgtgacgaa gtttttgttt
     6901 ttcagttaat tgcatgccgt attctgattg tttgccacgg ctattaggtc catgttgacc
     6961 tggtgcgtaa gggcgacgtg ctaattcttt tcctgtgcct gacaatgaga tgcctaagcg
     7021 acgagaaatt ttccatgatg gtcctgtata acgagacatt aaaatttcct ccaatgaata
     7081 taagtttttg gagtaaaata atccgttgaa aaattcataa tcatatagtt tgttcttcaa
     7141 tcttcacctt tgcagccgcg gttacacaat cgaacccgag acggggcaac aaactaggga
     7201 tgagttatta tttttctgct gcattatttt acacaaatta cagtatatga tagatcatcc
     7261 acgaatgtca agataattag tacataaaaa cgctttacaa acctaggctg tctttataaa
     7321 atgagagggt atgtgcaaat ggaaaaaagc gaaggaagtt taaggagaaa atcaatgaat
     7381 ccggtatcac cattatttga gaaaatcgat gcttcaattg aaaagaaggc gaatcttttt
     7441 gaaaacagct tttcacgtta cgctgttcga gcggttctcg cctgtttgtt tttgacttta
     7501 ggaacggctg tggcatttgc gatcgcgatg aagggggaag atatcgcgca tggtctagga
     7561 aagattttat acgcatttat gttcagctgg ggtctggtca tgattctata catgaacgcc
     7621 gaattaggaa catcgaatat gctttatatg accgtgggtg tttttcggca gagacttgct
     7681 ttaagtaagg cagccaagat tctatttgcc tgtatttttt tcaatatgat tggcggtata
     7741 ttatgtggat ttctaatttc tatgactacg ccatttcaag atttaacgtt ggacaacttt
     7801 atgttagaat caatagcagt aaaactagca aaaccttcag caacgatttt ggtggaaggg
     7861 atgtttgcga atatcgttgt caataccgct gtactgatca gcatgcggat gaaagaggat
     7921 gtcggaaaag tgatcacagt cgtattcatc attttcattt ttgctttttt aggatatgaa
     7981 cacgtgatag cgaattttcc tgcctttacg cttacttact ttgcttctca cggacagatg
     8041 gatggaatga cagtcagcaa tgtgttacat aatttgtttt ttgctttggc aggaaattat
     8101 attggtggcg gtttagtaat gggactggga tatgcttggc tgaatcagtc aaaatcatcc
     8161 tatgtggact aaaaagtcgt tcattaaagg ggaagtgcca tggaagtttt agtttttcca
     8221 aaaaatgagg aaaaagcgtt acaaatggaa aagtacatga aaaatctttt ttcctttgct
     8281 ggagtaccta agccggaacg cgctctttta caacaaccgt tactggatga gagcaaaaat
     8341 ttatctattt ctgaagtttt agcttttgtt cattattatt atgaacaaat cgaaagagag
     8401 tatcagtatg tagcgatcga tttagtccag aaaaacatca aacggcttac atttgaagac
     8461 ttagtcgtat tgttgccact agttcagaaa aaagaatggt gggacagcat cgactcttgg
     8521 agaaaagtat acagcacatg gtgtaaagcc tacccagagc agcgtgaaga agtctttcaa
     8581 ttgtttgaaa aaaaggaaga tttttggcta cgtcgaatag caatcacgtt gcaattaggt
     8641 tataaacagc aaacagacac aggactattg atccgagcaa tagacgaaga tcgtcaaaca
     8701 aaggaattct ttatccaaaa agcaattggt tgggcattac gcgattatag taagaccgat
     8761 gccgattggg taaaaagaca gctgacaaca gatttgagta agttagctgt tcgagaggca
     8821 agtaagtatt tataagaaag cgagacgaca agagatgaga cgcaaattat ttgcttttga
     8881 tattgatgga acgttgttag attcaaacaa acaagcttta gatagcacaa gagaagcatt
     8941 ggaaaaatta cgcaaagccg gtcatctggt cacgattgct acagggcgga gccgatttca
     9001 tgcgcaagag atcattcgcg atcttgattt tacgaactac attttatgta atggagcggc
     9061 cggtttttta gaccatgaac aagtatataa gaatttacta gatcgcgacc agttggatgc
     9121 gttcgttcaa gaagcgcatc atgagcagat cgatacagct tttgtcagct tagacaatat
     9181 caagcgttct tcttccaatg atgtaacggt gatggaagaa gcgatgcatt ctttcggtgc
     9241 tgtcttgcct gaattagatc agttttttgc agaggaacaa gaaatatacc aagcgttagc
     9301 atttttcgat caaacatatg atgggaagtt ctcttcatat gataaaattc gatttgtccg
     9361 ctggcatgag aacagtgtag atgtcgtacc taaaaatggt tctaaagcag cgacaattct
     9421 gcacttagca gaacaagtag gcatccctca tgaagacatc atcagctttg gcgatggaca
     9481 aaacgaccgt gaaatgttgc gcatgtcagg aattgggata gctatgggga atgctgtgcc
     9541 agaagtacaa gcagaagcta agatggtcac tgatacaaac gatcaggatg gaatttggaa
     9601 ggcattgaaa gagttaagcg tgatttaaaa gaggttgtga aaaagctgtt ctgcatcaag
     9661 taataagccc agccaaacaa aaatagctcc tcctattttt cgtttttctg ggcttattac
     9721 cgcagatgca agctttcgaa caccgtttaa ggaagaggtc gtgacagaag tggtcagttt
     9781 caagaaataa gaagggattt acgaaaattg cttctcaaat ttttgtaaaa ttacggctta
     9841 tttctgaaga agtagatgaa acaacgttta ttcgggaaat gggactgtga caagatgttt
     9901 gtcatagtcc cattttttta ttttattttt caaaactaag aaataccagg aaagagttcc
     9961 ttttgtttga ccttttcttt cttattgaaa acaactataa tatagagtga tgttaaaaga
    10021 gaagaggaat ttagtgtgcg ggtattgttt attggtgatg tagtaggttc aatgggacga
    10081 gagatgatta ctgaatatct tcctagattg aagaagaagt accgtccgca agtaacgatt
    10141 gtaaatggtg agaatgccgc ttctggacgt ggaatcacag aaaaaatcta taaaaaattt
    10201 ttgcaagacg gcgtagatgt cgtgacgatg gggaatcata cgtgggacaa ccgcggaatc
    10261 tttgagtttg taaatgaagc taaaaagatg gttcgtccgg caaattttcc ggaagggacg
    10321 cctggacaag ggatggtctt tgtcaaagtg aaccaagtcg aactggcagt gatcaacatg
    10381 caggctcgtt cttttatggt ggacttggat gatccttttc gtaaaatgaa tgagctggta
    10441 gaagaagcca gaaaacgtac gccgatcatt tttgtagatt ttcatggaga aacaacaagt
    10501 gaaaaacaag caatgggctg gtttttagat gggaaagttt cagcagtggt cggtacacat
    10561 acacacgttc agacaaatga tgcgcgaatt ttgccaagag gaacagcgta tctgacagac
    10621 gttgggatga caggtcccta tgatggtatt ttgggtatgc gcagagaacc tgtaatcgaa
    10681 aagtttctga ctgcattacc aaaacgtttt gaagttgtgg aacaaggccg atcgatttta
    10741 agcggctgta tcttggaatt ggatgatacg acaggtcatg caaaagaaat ccagctgatt
    10801 cagatcaacg atgatcgacc gttcatggag tagaaagaga ggcagttttt tgcagcagga
    10861 gccggaaatt caaaaagaag taaggaaact gacaaaactt ttaagagaga atgaaacgat
    10921 cattcgctac aaagaacttg aagaaaagat ccagcaaaac cagtatttag ctgagttgag
    10981 agaaaaaatc aaacaagcgc aaaaagatgc tgttcatttt gcccactatg acaaaccagc
    11041 agcagaaaaa gaagcaatca aacaagcgga tcaattcatg caagaatttg atcagcatcc
    11101 gcttgttgta gcttatagaa aacagctgtt ggaagcagat gatctgcttc atcatttaac
    11161 aactatgata caagaagaaa tcaatggaca aatagaggag gaaaaacatg cctcaaaaaa
    11221 ctaaaaatac tcctatgatg gaacaatatc taagcatcaa ggcccaatac caagatgctt
    11281 ttttgttcta tcgtttagga gacttttatg aactatttta cgaagatgcg atcaaagcgt
    11341 cacagctttt ggaattaacg ttgacgagca gaaatcgaaa tgcagaagaa cctattccta
    11401 tgtgcggtgt acctcaccat gcagcacaag gatatatcga tgccttgatt gaaaaaggct
    11461 ataaagtagc gatttgcgag caggtagaag atccaaagac gactaaaggg atggtcaagc
    11521 gcgaagtgat ccagctagtc actcctggta cagtcatgga cagcaaagga ttgtcggcaa
    11581 aagacaacaa ttttttaaca gctgtagtta gtcaaggaaa tgaatttggc tttgcttatg
    11641 ccgatttaag tacgggagaa ttgaagacgg ctcggttgca tgatgaagaa gctgttttaa
    11701 atgaagcctc tgcattgcag acaaaagaac tcgtattagg cagtgagatc actgataccc
    11761 ttcgtgaaca gctaagcggg cgtttagggc tagtcttttc tcaacaaaat gagatggaag
    11821 aaaacgcgga atttagcttt ttgacaagcg aactagtcga tccgctggaa aaagaagcaa
    11881 ccggcaaact attgacttat ttatcggtga cacaaaagcg gagtttagct catatccaaa
    11941 aagcagtaga ataccaacca gatcattttt tgaaaatgga ttattattca aaattcaacc
    12001 tagaattaag ccaatctatt cggactggga aaaagcacgg cactcttcta tggttgttag
    12061 atgaaacaaa aacagcaatg ggtggccgtt tactaaaaca atggctagac cgtccgctta
    12121 tccaggcaaa acaaattaaa gcaagacaag aaatggtcgc ttctttgcta gacagctatt
    12181 ttgaacgtat cgatttgcag tcagcattaa cgaaagtcta tgatctggaa cgcctagcag
    12241 gtcgagtagc tttcggtaat gtcaatgggc gagacttgat ccagctgaaa acttctttag
    12301 aacaagtgcc aatgatcaga gggttgattg aaggaatcga tcaaggtcaa tggcaaagtc
    12361 tgctttcaga aatgcagccg atggaccatc tcgtccaatt gatcgaccaa gctatccaag
    12421 atgatccgcc gttacaaata acggaaggaa acatcatcaa agatggattc aatgagcagc
    12481 tcgatatgta ccgttctgct atgagaaacg gaaagaaatg gttggcagaa cttgaagcaa
    12541 aagagcgtca agaaacagga atcaagaact taaaaatcgg atttaaccgc gttttcggtt
    12601 attatataga aattacaaaa gcaaatcttg gaaatgcaga actggagaag tatgaacgaa
    12661 aacaaacttt agcaaatgca gagcgtttca tcacaccaga actaaaagaa ttggagacac
    12721 aaatcctcga agcagaagaa aaatctgttg atttagaata tcagttgttt ttagctgtga
    12781 gagaagaagt caaaaaggct atccagccgc tacaagtact tgcaaaagca atcagtgcag
    12841 tagatgtgct acaaagtttt gcaacgatca gcgaacgcta tcagtatgtt cggccagaac
    12901 ttgtcagtga caagcatcag cttctgatca aagacggacg acatcctgta gtagaaaaag
    12961 tattaggaca tcaggaatat atccctaaca gcgtagaaat ggcagaagac gaaatgatct
    13021 tgctgattac aggaccgaat atgtctggta aaagtacgta tatgcgacag ttggcattaa
    13081 ctgttctgat ggcgcaaatg ggctgtttcg ttccggcaaa acaagcagtt ttacctattt
    13141 ttgatcggat ttttacccga attggtgcaa gtgacgactt aattgctgga caaagtactt
    13201 ttatggtaga gatgatggaa gccaatcaag ccctgcgtta tgctacgcca aacagtttga
    13261 ttttgtttga cgaactaggg cgaggaacag caacctatga cggaatggcg ttggcccaag
    13321 caatcatcga atatatccat cgtcatgtac aagcaaaaac actcttttct actcattatc
    13381 atgaactgac ggtacttgag aaagaacttc cgcagcttaa aaatgtccat gttggagccg
    13441 ttgaaaaaga cggagaagtt gtcttcttgc ataaaatgat ggatggacct gcggataaaa
    13501 gctacggcat acatgtggca aagatcgcag gattgccttc caatctacta gaacgagcag
    13561 cagatatcct gcatacgcta gaaaatggcg ataacggaca ccatactgtt ccagctgaag
    13621 tagaagagaa aactaaaaca gccaaacagg agcaaataaa accacctgca gaaaaagtaa
    13681 atcaagacca tatacaagac cgtgtaaaag aagaaaacca acagctttct ttattcagtg
    13741 aattatccga aaatgaaaca gaagtcattg aatcactgaa gcaaataaat ttactagaaa
    13801 tgacgccaat ggatgcacta aacacattat atgaattaca aaaacaactg taggaagaag
    13861 gcggatatcg atggccaaaa tccaagaatt atctgaacgt ttagctaatc agatcgctgc
    13921 gggagaagta gtggagcgac cagcttccgt tgtgaaagaa ttagtggaaa atgcaattga
    13981 cgccggcagt actcagattg atatcttagt tgaagaagcg ggactgcgga cgatccaagt
    14041 gattgataac ggagaaggca ttttggcaga cgacgtagag aatgccttca agcggcatgc
    14101 gacaagtaag atccataatc gcgacgatct ttttcgcata cgaacgctgg gctttcgagg
    14161 ggaagctttg ccaagtattg cctctgtttc tgaaatgact ctggaaacgg cgaatcagga
    14221 agaaaaacaa ggcacctatc tgtcgctgaa aggcggagaa gttattgagc atcgaccagc
    14281 accactgcgt caaggaacga agattaccgt cagcaatctg tttttcaata cgccagcccg
    14341 cttgaaatac gtgaaaacac tgcacacaga attagcgaac atcggggaca tcgtcaatcg
    14401 actggcattg agtcatccat cgattgcttt tcgtttagta catgatggaa acaaaatgct
    14461 ggcaactgct ggaaatggcg atctgaaaca aacaattgcg ggaatctacg gattatctac
    14521 cgcaaaaaaa atgctgaaaa tcgaaggaaa agatctagat tttgagttat ttggctatgt
    14581 ttcattgcct gaggtgactc gtgccagtag gaattactta tcgacgatca tcaatggacg
    14641 ttttatcaag aacttttcct tgaataaagc aatcgttgca ggttatgggt caaaattaat
    14701 ggtcggtcgt ttccctattg ctgtattgga aatcaaaatg gacccgcttt tagtagatgt
    14761 aaatgttcat ccgacgaagc aggaagtccg gctgtcgaag gaaaaagaac taacagagct
    14821 aattagtaac gccattcaaa aagcattgtc tcaagaaaat ctgattccaa gtgcagcgga
    14881 taatctacgg tttaagaaaa aagtacctga tcagcctaaa acagaacaaa tcgaaatcga
    14941 tttgtcctac gcttttgaag aagaaaaacc taaaaagcca agtagtttga attttgatcg
    15001 agagacaggt acctttttcg tagaagaact aaagaacaca tttgttgaca gaattgttga
    15061 taagtctgtg gaaaatgttg aaaactattc tggtttttct gaagaaaaca ccacttatcc
    15121 agaaaaaaca gcgaaaacgg tggataagtt tggtgaaaat gttgataact ttgtagaaaa
    15181 aacagtggaa aaatctgaaa agaaagaatt tcccattgtg gataacatgg agaaaacaaa
    15241 tgaagaacaa gcagataaat ccgtagaaga ggatgaaaag atttacgagg aagaattgac
    15301 actccatcct gaatttgatt tcactcaagg aaacgacaaa acattagaac aagccatcaa
    15361 gaaattagaa acggaaaaac cagtacaacg ttttcctcag ttggaatatt ttggacagat
    15421 gcacggaacg tatctttttg cccaaagtag cgatgggttg tacatcattg atcagcatgc
    15481 tgcccaagag cgcattaagt atgaatattt ccgggaaaaa attggggaag tatctgatga
    15541 tctgcaagaa ttgcttgttc catttgtgtt agactatcca aacagtgatg ctttgaaatt
    15601 aaaagagcaa aaacacttgc tagaagatgc agggatttat ttagaagaat ttggtcaaaa
    15661 cagctttatt gtacgggccc atcctacttg gtatcccaat ggagaagaag aatcgattat
    15721 tcgggagatg atcgatatgc tgctgacaac tggaagtgtg agtgtaaaaa aattccgtga
    15781 agcaacagca atcatgatga gttgcaaacg atcgattaaa gccaaccatt atttgaatga
    15841 agcacaagca cgtgtgctgc tgaaggatct ggcacgatgc gaaaatcctt tcaattgtcc
    15901 ccatggccgt ccagtattga ttcactttac caattcggat atggaaagga tgttcaaacg
    15961 aatccaagac ccgcattaag tcaaagagaa gagggaaaac atgaagattg tattagcatc
    16021 ccaatcacca agaagaaaag agttattggc tcatatcgtt tcagaattcg agattgaacc
    16081 tgctgatatc gatgaaacgc catttccaga cgaacagccc gtcgattatg ttcgtagaat
    16141 ggctgaagaa aaagcacgaa ctgtctggga acaagatgaa aaagaagacc atttagtgat
    16201 tgccagtgac acgactgttg ttctcaatca agagatcatg ggtaaaccag aagatctggc
    16261 tgaggctgaa ttcatgctga aaaaactaag cggggaaaca catactgtat atacagctgt
    16321 tgttttaaaa acaaaggaac gagaagaacg gatactagca gaagcacatg tcacttttta
    16381 tcctttgaca gacgaagaga tcaaacgcta cttggagacg ggggattatg cggataaggc
    16441 aggggcatac ggcatccaaa atgctgcgag tgtattcgtc aaagaaattt ctggagatta
    16501 ttacagtatc gtaggatttc cgattggtgc tgtcaatcag gcactgaaaa cattcattta
    16561 aacaaaaaag cggactatct gattttcgat tgaaaatcca tgatagcccg ttttttttat
    16621 tgaaaagatt acttatctac tctgactaag attttacttc agctttaccg actgcttcgc
    16681 ccgggcgaac aagtcctgat tggtcaagag aaagtcctgc aactttttca ttattggtca
    16741 aagcaacaat aatgtcagtt ttcttgccag cttgttctat ttctggcaaa tccatttcag
    16801 caatcagggt ctctggtgtg acaaggtatc cttctttcac gaatacattg aaaggaacac
    16861 cctttagttc aacagtatct aatcccatgt gaacaagtac ttccagcccc tcattagaaa
    16921 gaataccaat ggcatgtttt gtttcaaaga cactcgtcac tttaccgttt actggtgcat
    16981 agactttgcc gtttgacggt tcgacagcat aaccgtcacc catcattttt tgtgagaaaa
    17041 ctgggtcatt tacttcagtt atttccttga tggtccctgc agcgactgcg aataatgttt
    17101 cttttgttgc aactgccgta ccagttgctg aaacaggttc gaaagactgt ggcgttgctg
    17161 ttgcgccatt tgctgtttga agttgtacat cagcagcatt ttcaggaaca gcaggatcaa
    17221 tcttattcaa aattccctga cgtctaaaga tgatcgtcaa gccaaatgga atgatgacag
    17281 caatgatcat tgcgatgatg aaaggtaccc atgtttctgc ttggatagct agtattcctg
    17341 gcaaaccgcc tactccgatc gcgttagctc gaacacccat caagttagca aacatgccag
    17401 ctaatccaga gccgaccatt gcagcgacga aaggatagac gtatttcaaa ttgattccga
    17461 acatagcagg ttcagttacg cctaagtaac aagaaatcat tgctggaatc gagatttgtt
    17521 cttctttttt attcccacga tgtaagaaga tgatggctaa gactgcagag ccttgggcga
    17581 tatttgataa agcaatcatc ggccataaat tcgttgatcc gaaatctgca atcagctgca
    17641 tatcgatggc attggtcata tggtggagac cagtaatcac taaaggagca tacaggaatc
    17701 cgaatacggc actaaacaac cagctgatag aagacgtcaa tccagtattt acgatatttg
    17761 agatccagct tccgactgtc cagccgatag gacctaaaat aacgtgtgca gctaaaacag
    17821 ttggtaacaa tgaaaacaat ggaacaaaaa tcatcgagat cgactgaggg atatatttgc
    17881 ggaagaagat ttctagatac gctaacataa accctgccag catggctgga ataacttgcg
    17941 cttgataacc aatcatctgg acttgagcaa aaccaaaatc ccaaaatgga atatctgctg
    18001 cggctgtgga agctacacta taagcattta ataattgcgg agaaactaac gtaattccta
    18061 aaacgattcc taatatttgt gtagtaccca tcttttttgc aatactccaa gtgataccta
    18121 ctggaagaaa atggaaaatt gcttcaccgg gtaaccacaa aaaggcattg acaccattcc
    18181 aaaacttcga gtgttcaacg attgttccac caagactttc aaactggata ccttcaagta
    18241 catttctgaa tccaaggatc aatccaccga caatgattgc aggaatcagt ggtgtaaaga
    18301 tttctgctaa aacggcaata gcacgttgaa cgggatgaag gttttgtttt gcagctgctt
    18361 ttccttgttc tttggaaacc ccttcaactc cagatactgc aacgaagtcg ttataaaaag
    18421 tagaaacatc gtttcctatg atgacttgga actgtcctgc attcgtgaac atccctttta
    18481 cgctaggtat atcttcgatc gctttttcgt ccgcttttcc tggatcattc aggacaaaac
    18541 gcatacgagt cgcacaatga ctaactgcag caatattttc ttttccgccg atatcatgta
    18601 acagcttctc ggcatctgct tgatattttg ccatatcaat aaaccccttt ctcttaactt
    18661 gtctatgcaa gttgtttctt catttactat actttttttc ggagagaatt gcaagcgttt
    18721 tattaaaaag tacgagagaa aatgatatcg ttttaaaaag cttaaaataa gcttttctta
    18781 aaaataggaa cattgaaata caaaaagagc tgtagtatag tgaagaaatg atggaagaac
    18841 gagtgaggga aaaaacatga ataagttcaa tgaaattttt ttagatttgg aacagaaaat
    18901 tttagcgaaa gaatatcctc cgcattcgct tttacccagt gagaaccaat tgatcaaaat
    18961 ttatggtgtt tcacgagaaa caattagaaa agcgctgaat cttttgacta cggaaggtta
    19021 tattcaaaaa aaacaaggaa aaggttcgat cgtactggat cggaatcgct tcgactttcc
    19081 tatttctgga ttgactagct ataaagagct acaggagacc caacgtattc caagtgaaac
    19141 gatcatccat acgttagaag aaacagaagt gacaaaagca ttgaacgaga tcactggctg
    19201 ggagatcggt gcgcctgttt ggcatttgat acgcgaaaga aaaatcaatg gcgaagtagt
    19261 tatattagac accgattatc tattaaagga aatcgttcct attttaactc cagcacaagc
    19321 acaaggatcg atctatgaat attttgaaaa tgagctttct ttgacgattg cttacgcgca
    19381 aaaggagatc acagtagaag aagtaacgga aatcatgaaa acagcgatgg atcttcacga
    19441 tgacacttat gcagtagtag tcagaagcct cgttcatctg gaagataccc gctgtttcga
    19501 gtattctgag tcggttcacc gattagataa attccgtttc gttgagtttg cccgacgccg
    19561 aaaagtgtaa atagttcaaa agaaagcaaa ttgttggcat ctgtccactt tggcgctatg
    19621 ataggatcac aaagggggca tttttaatgg ataaaccaat aagaccagaa aaagaagaac
    19681 tgaaagaaaa gttatcaccc atggcttatg cagtcacaca agaaaatgca acagaacgac
    19741 catttagcgg gaaatatgat gatttttacg aaaaggggat ctatgtagat atcgtcagcg
    19801 gtgaaccgtt attcagctct gcagaaaaat atgatgcagg ttgtggctgg ccatcattca
    19861 gcaaaccaat aacgaaaaga caagtgcgcg aaaaagccga tttttctcat ggcatgcatc
    19921 gaatagaagt tcgaagcaaa gaagcagatt ctcatctcgg acatgtgttt accgatggac
    19981 cagtagatca aggtggtcta cgttactgca tcaattctgc ctcactgaaa ttcattccat
    20041 atgaaaagat ggatgaatta ggttatgggg aattcaaatc acttgtagaa taatggaaat
    20101 acacaaagag cggtacaata cggggattta ctcgatcgtc cgctcttttt ttattcaagc
    20161 gattgctatt tgaaagaaac gctccctaga ctcctttttt cacaaggtga aatgtgctac
    20221 aataaaggga acgtatgtgt gcaaaagaaa gaggactaga atatgtatga atatctgaca
    20281 ggtgtggtta cctttatcaa cccctattac atagtagtag aaaccaatgg tatcggctac
    20341 cagatcgcac taggaaatcc ttaccgctac agtagcaaac taaatcaaaa aataaaaatt
    20401 tatgtccatc aggtaatacg tgaagatgcg catcttctgt atggatttga ttcgctagaa
    20461 gaaaagcaac tgtttttgcg attagtaagt gtttcaggaa tcggtccgaa aagtgccctt
    20521 gcaatcatgg caagcgaaga ccactccggt ttgattcaag cagtagaatc cagtgatgtc
    20581 acttatttga cgaaatttcc aggagtaggg aaaaagacag cacaacaaat gatcttggat
    20641 ctaaaaggaa aattcggtga gctgagtatc gatacacctt ttagtctatt tgatgaagca
    20701 aacacaaaag acgctacagc tctatctgaa gcaatggaag ctctctcggc tcttggttac
    20761 agtgatcgag aaatcaaacg tgtagaaaaa caattaaaag aagtagataa tcagacaacg
    20821 gatgagtatc tacgccaagc actcaagcta atgatgaaaa aataaggagg agaccaaatg
    20881 acagatgaat acttgttatc accagaatct ggcgaaaatg aactgagttt ggaaaaatct
    20941 cttcgaccac agctgctttc tcaatatatc ggacagagaa aagtcaaaca agaacttcag
    21001 atttacatcg aagctgccag aaatcgggaa gaacctttag atcatgttct gttgtacggg
    21061 cctcctggat taggaaaaac gacgatggcc atggtcattg ctaatgagat gaccgtaaat
    21121 attcgtacga cgagtggccc tgctatcgaa cgtccgggtg acttagtagc aatcttgaat
    21181 gaactggaag caggggatgt cctgtttatc gatgagatcc atcgattacc tagagtggta
    21241 gaagagatgc tttattcagc tatggaagat ttctatgtag atatcatggt aggacaaggg
    21301 ccaaccgcac atccggttca tttccctctt cctcccttta ctttgattgg cgcgacgaca
    21361 cgggcaggta tgctttctgc acctttacga gatcgatttg gcatcatttc tcatatggaa
    21421 tattatgacg aacaggatct gcaggagatc gttttacgtt cttcggatat ttttcaaaca
    21481 gagatcattg aagaaggggc attggaaatt gctcgccgat cacgaggaac gcctagaatc
    21541 gctaatcgct tgctcaaacg ggttcgtgat tttgcgcaag tccaaggaga cgggaaaatc
    21601 gatcgtccta tagcggatca ggcacttact ttgctacagg tcgatcaggc tggattggat
    21661 tatgttgacc agaaattgtt gcgaacaatg atcgagttat atggcggcgg accagtaggg
    21721 ttaagtacgc tttcagtaaa cataggagaa gagcgtgaga cggtagaaga tatgtatgaa
    21781 ccgtatctga tccaaaaagg atttttaaag cgtacaccta gagggcgaat tgctactgct
    21841 tatgcctatg aacattttgg ttacgactat catatatgat ttttgctttt agattggagg
    21901 aaaatgaatg agtgatacaa cacgtacgaa acaattgatc aaaaacgtat tgattgattt
    21961 gtgccaaacg caatcttttc gaaaaatcag cgtccaaaaa atcgcaaacg aagcaaaaat
    22021 caatcgacaa acattttatt atcattttac agataaatac gatttgcttc gttgggtcta
    22081 ttgtgaggat gcattgcatt atttagatcc acaatgtctt tcacttgata attgggaaga
    22141 gcaagcattg aaaatgctga aagcaattgc tgagaagaaa gaattttatg caaatactgt
    22201 gctttctgac ccagaaattt tacaaaaaga gttttctcaa ctgatccaaa aatcatttat
    22261 caagattttt gaacaaatgg atgaagaaag acaactttct gcaagagaca aactatttta
    22321 tgctcgtttt ttttcttacg gttgcagtgg tgtattagtc aattggatca cgaatgaata
    22381 tgaagaaaca ccgatggaaa tcgctatgca gcttttccgt ttagccaagg atacggaatt
    22441 atattcttat cgtctttatg caaaagaaga gttggaagaa cagtaataga aaaatttttt
    22501 gttggaaaag caataatcgc taaaaggaga tggggaaatg acaaaaattt tatttgtttg
    22561 tttgggaaat atttgccgtt caccgatggc agaaggcttg ctgagaaaac gaatagcatt
    22621 agaaggaagg gaagcagaat tttttgtgga ttctgctgct acaagcacgt atgaagttgg
    22681 aaagacaccg caccctggaa cgaaaaaaat attaaaccaa gaaaaagtag atatgacgaa
    22741 tatggttgct cggcaaatca caccccacga ttttgaaact tttgattgga tcattggcat
    22801 ggatcaagaa aatgtagaag aactgaaacg aagagcacca cgctcagcgc aggggaaaat
    22861 ccatttgttt ttaagtagca ttcctggaaa agaaaaagaa aatgttcccg acccatacta
    22921 taccaataac tttgatgaga catatcactt gatcacagaa ggcctgaatc attggtggaa
    22981 tatttggatt gatgaaaata aaataacaaa caatttgtaa agttaacgct ttctttttaa
    23041 ggcgatgtta gagcatttcc ttgttttttt gtaaaatgtt aggtaaaata aaattaatta
    23101 agtaacactt tcttagctaa aggaggagtg cattcgatga acgaagagac agtatcgcca
    23161 atgaagaagc ttacgaagtt agttactcat acttcgtatc aactttttga ttcttttgta
    23221 aatgacactg aagaacatgc taagaatgta cgattgcaag gaaagtttat tcttgagctg
    23281 caaaaagctg ctactgaaca tagtctagtt atcttacaag taagagcaaa tcgtttgagt
    23341 agcaagtatg aaacgatctc aggctgggtg gcgacaaaaa cggtcgaaaa agaccgagtg
    23401 atgatccgcc tacaaaacga agagcagcag atccggatgg ttcctgtaga acaaattgaa
    23461 aaaataacaa tcctttcacc agcaggggaa caggaacggt tatcaaaata atttatacca
    23521 gatagaaaag cacgtatgag cttgtaccat caatcattac taatcaaaaa taaccgaatg
    23581 ctttaagctg atatcagtaa aacggaacgt tttaggatga gtatcacagc atgacagcat
    23641 tgggttgatt agttgaatcg attggatggt acaagttttt ttgataaaaa aaggtcctgc
    23701 tagccgggga gctagcagga taaggagtta aaaatgaaaa agtgttgtta gggttgtttg
    23761 ttggtatgct tatataatag tcactagttg tgaatatttt gtgacgaaag ttttcgaaag
    23821 ttgttaagtt tttttgcgaa aactattttt atatcatata tatgtctaaa atctatatta
    23881 taaaagaaat gttgtttgat cagcttctct tcggtattag ttcaaaacaa aacggcaaag
    23941 tatatggagc tactttgccg ttttgtttct ctaatactca ggtcagttta tagacgctca
    24001 ataaacggtg ttcaaaagcc agtttctcgg tattaagtca aatttatgca aaaatgtgag
    24061 aagccatttt caaaaaatat ttcttaatac tcgaaactga gcggcttttt cacgacctct
    24121 caataaacag tgttcaagag acagcctatc ggcaataagg ctgaacgtct ctttcacaac
    24181 ctcttttatc aatctgctac ttcttctttc ttagcgaaac tccaaatcag tccgataaca
    24241 aaaccgataa ctgctggtac gatccacccc atccccaatt tgaaaaacgg caagtagctt
    24301 tccgcaacat ataaaagatt ttgaacaata ggtgtttgtt tgatagtttc tgggcaagcg
    24361 ttcaacccat caaaaatcga agcaatcaat gtaaaataag tagtcattcg ataaacagcc
    24421 gtacgctgct taaacaaagg accaactaac actagtaaaa tcagtgtcat tgctaatgga
    24481 tagatgaaca tcaaaacagg caacgaaaat tgaatgatat tcgtcaagcc tacgtttgcc
    24541 gctaaacaag gaaggatact agcaagtgca acaaagaatg cgtaatttct ttgcggaaac
    24601 aaatctgtga atgtttctgc aaaagctgta atcaaaccaa taccggtctt caagcaagct
    24661 aatataacga taaatgctaa taagatactg ccataagtac ctaaataatg atcagcaatc
    24721 tgagccagcg tgatcccgcc attttcactt agagcaaagc tacctaagct catagcacct
    24781 aaataagcga gcaaagtata aatgattccc atcaatataa tgctgatcgc accagatttg
    24841 atcgtgtctt tagcgatttc agcaggtttt gttacaccca tgttttggat tgttgtgaca
    24901 atgatgatcc caaatgctaa agcagcaagc gcatctaatg tgttatatcc ttgagtaaac
    24961 cctgtgaaaa aagcatgctc ttgataattt ggctgaatag gcgcttgatt tacagaacct
    25021 agtggattca aaaaagcaag gagaagcaaa gcacctaata aaaccaaaaa tgcaggattc
    25081 aggaactttc caacataatc taatatcttt gtcggttttc ttgaaaacca ccaagccagc
    25141 acaaaaaata caatactgaa aattgctaga tatagcgtct gtctgctagg atcgataaat
    25201 ggtgctaaac caatctcgaa agatgtggtt gcaaggcgag gtaaagcaaa aaacggtccg
    25261 ataactaagt aaagcaagat ggtaaaaacc atcgcatatg tttgatttat ccgttttgct
    25321 aattcatata cacctgagct tttcgataca ccaatggcaa tcacccctaa gaagggaaga
    25381 ccgataccgg taaccaagaa acctagattc gcccaaaagg tagcagcgcc tgcttcttga
    25441 cccaaatgaa cgggaaagat aagatttcca gcaccaaaaa atagaccgaa aagcatggag
    25501 cctataaaaa gatagtcttt aaatgataat ttttttgtca tacataactc ctttacggtt
    25561 tttttaaaag taacaaaatt tcgaaaaata tgcaacaatt ttcagaaaat ttcctatata
    25621 aaacgacggt tatttaataa cgtaagtgat aaaataaaag cgctgaatca tctgaggagg
    25681 aatcatcgtg acaaagtcga gattagaagc atttactgat ggagtggtag ccattgtttt
    25741 gagagttctt gtattagaga tcaagatccc taatacatct agctttcatt ctttatgggt
    25801 catccgaaat acattattag cttataccat cagctttata tttgttgcgg tgatctgggt
    25861 gaaccatcat cgtatatttc agatggcaga acgaattaat taccgagtgg tctggtcgaa
    25921 tatctttttg ctcttttggc tgactttatg tccttctgtt actagttggg tcgggcgtaa
    25981 ccctgaagct ttttggcctg agatggctta tgtcttagta tacacgatgt ggagtttttc
    26041 ttttggtatc ttgatgaggc aaatcatgaa agcaaataaa ccagattccc atgtcgtgag
    26101 agttttgtca aaggaccgtc gaagtcttat ctccatgatg attaacttat gcttgattgc
    26161 cggtgtcttt tttgtaccgt tgatcgggtt attcggacgt ttttttgtat ctggtatttg
    26221 gattttttcc tataaaaaag cagatgccta ttatcagcgg ttgtttccgg ggaaatagaa
    26281 aagaggctgg gacagaagct tttaactcca agaaataagc cgaaatttca caaaaattta
    26341 agaagcaatt ttcgtaaatt ccttcttatt tctcgaagct gaccacttct gtcacaacta
    26401 ctttcttact tcttacgttc atatttcata tgatagatta gtaatagaag caagaaccac
    26461 acaggtgtta gtaataaagc gattctcgta tcttttccta ggaacaagac gacacacaca
    26521 aaggccatgt aaagaaatac cagataatta ctaaatgggt acaacggcat tttaaatgaa
    26581 tgagcttttt tgccgaggat acttttacga tatttcatgt gcgtgtagac aagcatactc
    26641 cagataaaca agaaacaagt agtggcaata ctgcttacta gtgtgaatac ttcactaggc
    26701 atcacgtaat tcaagataac agaaatgaaa atgacgatag tagaaaatgt caaagcattc
    26761 cctggcacat gatgtgttgt taactttttg aattttttag gggcactgcc ttcttgcgct
    26821 aaagaacgaa gcatacgtcc tgtactatat attgcactgt tgcaggcaga agctgctgca
    26881 cttaatacga ctaaattgat caaggaagca gcaatcgtga tcccaatttc cgaaaatact
    26941 tctacaaatg gactgttatc tggattcaag ctgttccatg gatagattgc cataatgaca
    27001 aaaagagatc ctaagtaaaa taagatgatc ctgataggaa tattattgat agcttccggc
    27061 aacacttttt caggattttc tgtttctcca gcgactaatc caactaattc tatcccagca
    27121 aaagcaaagg ttaccatttg gaaagctaag ataaatcctt ttgcaccagt tggaaagaat
    27181 ccgccatgag accatagatt cgtgatactg gctgtgccaa tatctgtcgt atagtgggtg
    27241 aggatcatat aagctcctac gaggataaag gcaatgatcg ctacaacttt gatcaatgca
    27301 aaccaaaact ctaactcacc aaacaatgag acagccacca tattcaagca aagaagaaga
    27361 cctaatgcaa tcagttcagg aagccactga ggaacaccag gcgcccagta gcgtacatac
    27421 attccgatgg ctgttaaatc ggccattgca atcgataccc agcagaacca atacgtccag
    27481 ccagtgatga aagccgcttt tttgccaaga tattctgcaa caaaatccag aaaagaatgg
    27541 ttattggtgt ttgataacag aagttcgcct aacgcacgca taatgaaaaa acagatgcat
    27601 ccagtaataa gataggcgag caatatcgaa gggccagcta gctggataga ctttccggaa
    27661 cctaaaaaca accccgttcc aatcgctccg ccaatagaaa tcagttggac atgtctattt
    27721 ttcagtccac gctgcagttc ttgctcttca ttcattttta aatcatccta actaaaaaag
    27781 tttaattact tccatattaa aagagttttc tgaaaaacac aactaagatt ccaaaatttt
    27841 cagaaattta ccttttatga gtaattcata aaaaagaact gcggtaaaca ccgcagctct
    27901 ttgtcccaat cagctattga tcctaagcat ttaaaatacg tgaaaggaat tcttttgtac
    27961 gttcttcttt cggatggaca aagatatctt ctggtgtacc ttcttctgca atcacacctt
    28021 tatccataaa aatgacacgg tcagatacat cttttgcaaa ttccatctca tgcgtcacga
    28081 taagcattgt caagccagta tgtgctaagt ctttgatcgt attaagcact tcgccaacca
    28141 tttcagggtc taacgccgaa gtaggttcgt cgaatagcaa tacatctgga ttcattgata
    28201 atgcccgagc aattgcgaca cgctgtttct gtccaccaga taattgtgct ggtttggctt
    28261 cgatataacg atccatacca actttttcca gattttcgat tgctactttt ttggcagttt
    28321 ctttatctcg tttcaggaca gttgtttgac cagacataca gttttccaag acattcatat
    28381 tgttgaacag gttgaaggat tggaaaacca tacctagatt cgttcgatat ttaggtaaat
    28441 tatatttagg agctaataca ttttctccat tataaatgat ctcgccgcct gttggttttt
    28501 ccagtaaatt gatacagcgc aagaaagtag atttaccaga accggaagaa ccgatgatcg
    28561 tcacaacttc acctttattg actgttacgc tgatatcttt caatacttca ttttcaccga
    28621 agctttttct tagatgtttg atttcaataa ttttttcatt catgattctt cctccttatt
    28681 cctgcaaatt tgaattgtcg atttcttcaa gcttggtata agctgaagga ccatcgattt
    28741 tcttttctac cgcccgcagg attctggtga tcgtaaaggt catgactaag tagatgatcc
    28801 cgacgatcgt aaatgtttgg aagaattgga aattcgcacc ggaagcagag tttccttgga
    28861 agaacaaatc agctacgccg attacactta aaactgctgt atctttgata ttgataacaa
    28921 actcatttcc tgttgctggc aagatattcc gtaaaacttg tggaataaca acttttctca
    28981 tcgtttggcc atgggtcata ccaatggctt gcgcagcttc gaactgccct tcatctactg
    29041 caaaaatccc cccacgaacg atctctgtca tgtaagctcc agtattgaga gagacaataa
    29101 acaaagcggc aatcgttcgg ttcaatgaaa tgccaaaagc gagtgccaga ccgtagaaga
    29161 tgaccattgc ttgtaccatc atcggtgttc cgcggaatac ttcgacatag atagaaagaa
    29221 gtgcatttcc gattttttgg aagaagcgag tcagtgcatt tcctgattca ggtaccgaac
    29281 ggaagacacc aatcagtaat ccaagtgtag taccgacaac tgtcccgatc aatgcgatga
    29341 acaaggtaag tccagcacct cgtaagaaca tattgccgta ttgtttccaa atcgtagtga
    29401 aatcagaaat gatgccaggt ttttcttcac tatcagttgc agcaggctga tctttgactg
    29461 cttggtccat aattttggtt cgttcatcgt tagagatgcc agaaagaatc tggtttactt
    29521 tttggatatc cgggtcgcct ttacgcatac caacagaaat ctgaacatct tccgggtttg
    29581 tctggaagcc atctgcaccg ctgaattcaa gcatttccaa gtctttgttc acactagttg
    29641 ccgttacacc ttccggacgt tcgctgacat aaccatcgat cattccagaa gctaaagctg
    29701 ttctcattgc tgagaaatta tccattgctt gttgtttctg cacatctggt atctgatcga
    29761 tcacagtata gtggaatgta tttaactgag cagtaatctt tgctccagaa agatctgcta
    29821 aactttttgc ttttgcatat ttgctatctt tttgaactac tactactaat tgtgattcgt
    29881 aataaggatc ggtaaaatca atttcttttc gtcgttcagc agtagggctc attccggcga
    29941 tgatcgcgtc gatcttgcct gattgaaggg caggagccag accatcccat ttcgtttgga
    30001 cgatgactaa ttttttccct aaaccatctg ctactttttt agcgatttga acatcataac
    30061 cgccggcgta actattttgg ccttgaattg gcaaggcatt attctcatct gtttgttgag
    30121 accagttgaa gggtgcatac ccagcctcca tacctacgcg aaactcatcg ctcggtgttt
    30181 tttcatctgc atttgccaga gatcccacac tgaatagtga gaagacaaaa gcaaatagta
    30241 cggcaactga aaccatattt tttttcattt ttttctccct ttctgattat atgatacacg
    30301 agcggtctag atgaaatgcg gtaaagcttt atctgaaaaa ataaaaagcg accgatttat
    30361 agatataaaa aggccgctaa aattccataa ggtgataaac ctctcacaaa acatagcgca
    30421 acttagcatc actgctaaga cagtccgcaa gctcttaact tgcgtcccaa catacactcc
    30481 aagcaaaaaa gtgcacattt cggcgatcct ccctattcct gcttctccat gtttgcttca
    30541 ctccacaggg ttaatgattc tcgcgacctc tacctcattg attgaggcat ctcgtattca
    30601 gttatttata caaaaaaagt ttacgatact cgagaggctt tgtcaatcta taactgctgt
    30661 tttttgaaaa aatactgata aatatagaaa tctttacttt taccgaaaaa acaatcaaaa
    30721 tgtgaatcat ctatccattg caaacgatag tataattata cacaaaaatg tatgtaatac
    30781 aataaaaaaa gaactagaac agaaaatttc ctgtgtccag ttccatctcg atcatttaag
    30841 ctgccgcttt ctttggctca cgtcctagga tcgcttgcaa aagaacaacg acgccataaa
    30901 tggctaatat tagataaatc aaacttgagt attgggtgat tccccagccc catgttcgat
    30961 atacggctaa tgtaatggct aaaacaattg cggcaatcac gttgaataaa gcaaaagccc
    31021 aaagatttcc attttctttg cgtacaagat aaaaaacaga gaaataaacg cttaacgcta
    31081 accaggcgat acaagccaga ataacgcccc aataaataaa gaatggtaac atatcgattc
    31141 acctcacttt tatacagcag tattgtaaca cgttttcatt cttttgtcag tgaacattgt
    31201 gataaattgt tcaaatgccc ataacacttt tttattccca tttttctgac ttttcaacaa
    31261 taaatttgtt cgagtatggg ctataatgga aagtgaggtg acaaaacatg aaacaattaa
    31321 cagtagacga agcagcaaaa gagatattag cttatcagca aattacattt ttgaccggtg
    31381 caggggtatc cacaccttct ggagtgccag actatcgatc tttgtcagga atctaccacg
    31441 ggatggagca gccggaatac ttgctgagtc atcaagcaat ggtgaatgaa ccggataaat
    31501 tctatcaatt tgtcaaaaag atttatcatc ctgaagcaaa acccaatatc attcatttgg
    31561 aaatggcaca tctagcgaaa gaaaaaaatg tttgggttgt ttcccaaaac atcgacggtt
    31621 tgcacgcaaa ggctaaaagc ccgcaactcg tcaattttca tggaagtttg tatcaatgtc
    31681 attgtcgaaa atgtcaacaa acggtagact ggaaagaata tcttcacagc gacaaacatc
    31741 aagcatgcgg aggacaaata cgcccagaca tcgtgttata tggtgaaggg tttcaagatg
    31801 gagtcatgga gcaggcggct tatgccgtca gccaagcaga gctgatcgtc attgttggaa
    31861 cgagttttca agttcatccg ttttgtgatc tggtacagtt tcgccagccg tcagccaaaa
    31921 tcttagtaat caatcaaacg cctgtttatt tagcggagcc ttatacgttt attcaagaag
    31981 acggagcggt tgttttcaaa aaaatacaag aattaggagc agattatgac tgaattcaaa
    32041 accgaaaaag aaaaaatgat tgcaggcgag ctttattttg ccaatgatcc tgagctggtt
    32101 gccgaccgta tgtttgcaag aagtcaaagc caaatcatca accaagcaga atcagcggaa
    32161 ctgcgatccc aattgttgaa agagactttt ggccgtacag ggaaaaaaat ctatatggag
    32221 ccagttatca attttgatta cggatataat atatttgttg gggagaattt ttatgcaaat
    32281 ttcaattgta catttttaga tgtcagcacg attgaaatcg gtgataactg catgttcgct
    32341 ccgaatgtcc agctttacac ggcgacgcat ccactacatc cagtgaaacg taacagcggt
    32401 ctggagtacg caaaacccat taaaatcggt aataatgttt ggttaggcgg aggagttatc
    32461 gtcacgccgg gtgtcactct aggggacaac gtagtagtcg gcgcaggttc ggttgtgacc
    32521 aaatcttttc ccgacaatgt agtgattgca ggaaatcctg ctcgtatcat caagacagta
    32581 gaggaagaac taacagaaga gtctcttgaa acattacgtc ataagatcga tatgatcgat
    32641 cgagagttga ttgctttatt agaaaaaaga atggatacgg taactaaaat aggacaagtg
    32701 aaaaaagaag aaaaccaagc agtttatgat gcaaaaagag agcagcaagt attggataaa
    32761 gttgttagtc tgctgaaaaa taagggatat aaggaaacaa taacagatac gtatgttgat
    32821 ttgatgaaac actcgcgcga ataccaaaac aaaatgaagg aggaataagt gtgatcgaag
    32881 gagagacacg gcgaaaacaa atcatgcaaa ccttggcaaa tacagaaaaa ccagtgagtg
    32941 ctagcaagtt tgcgaaaact tttggagtca gccgtcagat cattgtaggc gatattgctt
    33001 tactacgagc tgcaggtaaa agtatcgtgg caacagcaag aggttatatc ctcgaaacag
    33061 aagaaaccaa tactgggcac atcagtaaaa tcgctgtcca gcatggaaaa gatcaaacag
    33121 aagaagaact gagactaatc gttgaaaatg gtggtgaaat cattgatgta attgtggaac
    33181 atccccttta cggtgagtta gcaggaactt tgcacatcaa aacgccacaa gatatcgatt
    33241 cctttatgag acgttatcaa aaaagtaatg ctactttgtt atccgaatta acaggcggca
    33301 tccatctgca tacgatccgc tacccagaaa agcatatgct gaaaaacatc aaacaaaaac
    33361 tagctgaagc aggaatcctc tacgaaggat aacatcgtag ctgctacttt ttatcctaga
    33421 aactattcta tcatctctga gataaacacc catatggaag aaataagaaa aaataagaaa
    33481 agatgcgtat ccaatacaca gaattttttg gaattgcgat ttctagatgt ctatctaaat
    33541 ttcgattagt ggagaaacat aaaaaaggga agtgcagctt ttgagcactc ccctttttta
    33601 taaagctttc ttgtagcata gtatcgaagg aacctgccaa ttctctactc gatcaccaat
    33661 acgccttctt ttagtgcaaa tggattttga tcgttgatgt ggtcatagaa catcacaccg
    33721 ttgatatggt cgatttcatg ttgaacgacg atcgattcat aattcttcaa acgaatcttg
    33781 tgtttttcac cattcatatc ataataagac acagtgattt tggcatggcg aacaacatat
    33841 cctggtactt ctcgatcaac agataaacag ccttcacctt cccctaaaca agcatcttga
    33901 acagaatggc tcaaaatttt aggattatac ataacagtac ttagagaagg tccatctgcc
    33961 tctgggtcag gacttggtac gtgaacagca atgattcttt tagaaatatc taattgagga
    34021 gcagctaagc caacaccgcc tcgtaagtgt agttcttcag ctttgattgg atcttggctg
    34081 ttttttaaga actcaagcat ctcttttcct aaagagatat cttcttcgct taatggaaga
    34141 gaaacttctt ttgcaacttc gcgtaaggtc ggatttcctt cacgaatgat atcatccatt
    34201 gtaatcatgg aatatgtacc cctttcttaa aagactgtgt ttcaagtcta ttttcgcatt
    34261 atagtttacc acaaaaatgg aaaagtagta gtaagattgt ttgaaaacta ccttttctta
    34321 atgattaact acttgatttt caacccggtt tcaggtaaag tatagctgtg tgctctaggc
    34381 acatgacctg ccacttggtt aagcgaatca ccaacgtttt actcagtcgc aggggaataa
    34441 tcgaaagagg tgaaagaaat ggcaatttca aaagaacgca agaatgaaat catcaacgaa
    34501 tacgctcgcc atgaagggga tactggttct ccagaagtac aaatcgctgt attaactgaa
    34561 gaaatcaacc acttgaatga acatgcacgc gtgcacaaaa aagaccatca ttcataccgc
    34621 ggcctaatga aaaaagttgg tcaccgccgt aacttgttag cttacttacg taaaactgac
    34681 gttcaacgtt accgcgaatt gatcaaacgt ttaggcttgc gtcgttaatt ttagtcaaaa
    34741 agagtgggat tcacatgaat ctcacttttt ttactcgtat atcaattaag taaaattccg
    34801 tgatcaagct agatggactc ctactaacca aatgaaagct gctcaaaaga cggctttcgg
    34861 cagttttcat ttgtttagta gcgtcttgct ttcacgagaa ggaaaaggag aagttatgtc
    34921 agaaaaacaa gtattcaaaa cgacatgggg cggccgtccc ttacaaatcg aagtcggtca
    34981 gctagcaaaa caagcaaacg gagccgtttt agtccgttat ggagatacag tagtcttaag
    35041 tgcagctgtg gcctctaaag aagcaaaaga tacagacttt ttcccattaa cgatcaatta
    35101 tgaagaaaaa atgtatgcag cagggaaaat ccctggaggt ttcatcaaac gagaaggccg
    35161 tccaagtacg gaagcaacat tgactgcccg tttgatcgac cgtccaattc gcccgatgtt
    35221 tgcagaaggc ttccgtaatg aagtccaagt aacgaacatc gtgatgagtg tagaaacaga
    35281 ctgctcgcca gctatggcag caatgctagg ttcttcttta gcactatcaa tctcagatat
    35341 cccatttgac ggcccaatcg caggcgtaga agttggtcgt gtaaatggcg agtacgtctt
    35401 gaatccaaca gtagaacaag cagaacaaac agatatcgaa ttgactgttg ccggaacgaa
    35461 acaagccatc aatatggttg aaagcggcgc gaaagaagtt tcagaagaag acatgcttgg
    35521 tgcattgctg tttggttttg acgctatcaa agagttagtt gctttccaag aagaaatcgt
    35581 tcaagcagta gggaaagaaa aaatggaagt gactttactg caagttgatg aagtattgaa
    35641 aaaagaaatc tttgatgctt cttacgctac aatgaaagca gcagtaatga ctgaagaaaa
    35701 acttgctcgc gaagataata tcgagcaggt gaagatcgat atccgcgaag cttatgctga
    35761 gaaatttgca ggtcatgaag atgaagatca cttgttgaaa gaagttaaac aaatcacaga
    35821 agatcttgaa aaagatgtcg tacgtgaatt gatcaccatc gataagatcc gtcctgacgg
    35881 gcgtaaattg gatgaaatcc gtccattgtc atcagaggtc agcttattgc cacgtgtaca
    35941 tggatcaggt ttgttcacac gaggacaaac acaagcttta tcagcttgta cactcgcacc
    36001 tctaggagag caccaaatca ttgatggttt aggggtggaa gtcagcaaac gattcatcca
    36061 ccactacaat ttccctcaat tttctgttgg ttctacagga cgtgcaggct ctccagggcg
    36121 tcgggaaatc ggacatggcg cattaggtga acgtgcgtta gcacaagtaa tcccttcaga
    36181 agaagaattt ccttatacga tccgtttagt agcagaagta ttggaatcaa atggttcttc
    36241 ttcccaagca agtatctgtg ctggcacatt agcattgatg gatgcaggtg taccaatcaa
    36301 agcgccagtc gccggtattg cgatgggact tgtttctgat ggtgaaaact atacgatcct
    36361 aactgatatc caagggctgg aagatcatct tggcgatatg gactttaaag ttgccggtac
    36421 aaaagatggt atcactgctt tacagatgga tatcaagatt caaggaatca cagaacaaat
    36481 cctaactgaa gcattaactc aagcaaaaca agcacgtatg gaaatcttag aagaattgac
    36541 ttcaacgatt gctgctccac gtgaagaatt aagccaatat gcaccaaaaa tcgaaatgat
    36601 ccagatcgaa cctgctaaaa tcaaagatgt tatcggtaaa ggcggcgata cgatcaacgg
    36661 aatcatcgat gaaactggtg tgaaaatcga tatcgatcaa gatggaaaag taagtattgc
    36721 ttcagctgat gcagaaatga tcaaaaaagc tatcaagatc atcgaagatt tgaccaaaga
    36781 agtcaaagtt ggtgaagttt atctagggaa agttgtccgt atcgaaaaat tcggtgcatt
    36841 cgtcaacttg atcaaaggaa aagatggact tgtccatatt tcccaattag caaacgatcg
    36901 tgtcaataaa gtcgaagatg ttgttaaact aggtgatgag atccttgtga aagtaactga
    36961 aatcgataaa caaggacgcg taaatctgtc aagaaaagca atgttgaacg aagatggttc
    37021 aacgaaaaat gaagaaaaat aatttaattc ataggaataa tgtacttgtg atcgtgaagg
    37081 tgacaggggg acaataatta ttcgaaaaaa ggggactgtg acaaaagccg tgtcacagtc
    37141 cttttttttc gaataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaag
    37201 ttaaaaacat taggagctgt ttttatttgg ctgttcttat tacttgatgc agaacagctt
    37261 ttttacaacc tctccaataa acgatgttca atcagctgtc cctcgatatt agctcaaaaa
    37321 acggcaaaat atgaggaact atttttccgt ttgtttctct aatactcagg tcaaaccgct
    37381 gatttcacaa cctctgattc acggtgttcc agaagcagct tctacggaaa taagctgaac
    37441 acttttgtta caacttcttt ttttctggag tttcttggcg catatttttg gagtccttct
    37501 gaataaagcg taaaataaaa gtgagaaata tggataaagg agggaaagga gatgctgaat
    37561 tttttatggt cgttatttat cggcggtata ttaggtttga tagctggcgc aattttagga
    37621 aaggaagtcc cgggaggcgt atctggaaat atcattatcg gttttttagg tagttggctc
    37681 ggcgaatttt tactaggacc actgggaccg gaactcggcg gtttttattt gataccagca
    37741 ttattgggtg caattttttg ccttgctctt tattcatttg tggcaagacg tatcagagaa
    37801 cgtccaaaga aagaataatt ttattcaaat aagttgacac ttcagtgtgt tacttggata
    37861 attagaagca atttaggaaa gcgggtgtca tggataagaa tatcatctat cattcaagct
    37921 tacctaaagg atatccatat aacaagaaat gagggaaaaa cgtggcaaca acgcacaata
    37981 ccaaaaagat atcaatgact ggcctgcttg tagcgatggg agtcgtctac ggagacatcg
    38041 ggacaagtcc gctttacgtt atgaaatcga ttgttgaaga taatggcgga ttgtcaggaa
    38101 tcaatcctga ttttgttatc ggatcagtct cattgatttt ttggacattg actctactga
    38161 caacaatcaa atatgtcgtg attgcattga atgcagataa ccatggtgaa ggtggaattt
    38221 tctcactcta cacacttgtc cgaaaaggtg ggaaatattt gatcattcct gcaatgatcg
    38281 gaggagctgc cttactagca gatggggtat tgacccctgc tgtgacggta acgactgcga
    38341 ttgaaggctt gcgaggaatt ccgcagtttt ttgaccggtt cggtaataac cagaacgtga
    38401 tcgtcattat cactttagtt attatttttg ttttattcat ggtccaacgc tttggtacag
    38461 atattgtcgg aaaagcattc ggaccaatca tgttcgcttg gtttactttt ttaggcgtaa
    38521 tgggattaat gaacttcagt caagactgga cagttatccg tgcactgaat ccttattatg
    38581 ctatccagct gctttttagt gcagataaca aattaggctt gttcattctt ggaaatattt
    38641 tcctggctac gacaggtgca gaggcgcttt attccgatct tggacatgtc ggcaaacgaa
    38701 atattcgtct gagctggcct tacgtcaaaa tttgtttggt tatcaattat tttgggcagg
    38761 ctgcgtggtt gttgaatgtt tatcaaaacc ctgaagcaca gcaaatcaag aacctgaatc
    38821 cattcttcca aatgatgcct caaagttgga tcgtgatcgg cgttgctttt gcaacagtcg
    38881 ctgcagtcat tgcttcgcaa gcattgatta caggttcgtt tacgctagtt tcagaagcaa
    38941 tcaagttgaa actgctgcct cggttaaaga tcatgtatcc tgggaacagt atcgggcaaa
    39001 tgtatatccc agcagtcaat ctgattttat ggatcgcctg ttctctagtg gttgtcacat
    39061 tccgaacgtc tcatcatatg gaagctgctt acggtctatc gatcactgtg acgatgttga
    39121 tgacaacagc actgctttac ttttatctta tccaaaatgg gcattctcgt tggcttgcat
    39181 atttagttac ctttttcttc ggtgcgatag aagtcgtttt ctttatctca agtgttgtca
    39241 aattcttccg aggcggtttt gtagccgttc tgattggttt gatcatctta gtcgtgatgt
    39301 ttatttggga acaagggaac attattcgcg aatcgtcaac cgaggatgtg tctttgagag
    39361 attatatccc gcagcttaga gaattacgtg atgatacttc tttaccaatc tatcagacaa
    39421 atgtggtctt cttggttcct gatatggctg aagataaagt tggccgacag ttcatctatt
    39481 cgattttaga taaacggcct aaaagagcac gagtctattg gtttgttcat gtggaagtga
    39541 cggatgagcc atatacgaaa gaatatcaag tagatatgat ggacactgat tttattgtcc
    39601 aagtgaatct gttcttaggt ttcagggtcc aacaggaaat caacgtatat gttcgccaag
    39661 tcatccatga tttgatgaaa caaggccgtt tgccaaaaca gccacaaaga tactctctga
    39721 caccagggcg agaagttggc gatttccaat ttgtcctgat tgatgaagta gtttccaatg
    39781 tgacaactct aggaaaatgg caacgtcaaa tcatgcaggc aaaactggca atcaaaaaaa
    39841 tcgctacctc gccagaaaca tggtttgggc tagagtacag cgaagtaaaa catgaaactg
    39901 ttccgcttat cattggaaat actcggaaaa catggttgaa agagcggttg tgaaagtagc
    39961 gctttgacct gaactaatgt cgaatgtcag ctacttgaac accgtttatc agaggtcatg
    40021 aaaaagctgt actgcaacaa gcagtaagaa cgagcagata aaaacagctt ctcatgtttt
    40081 tcatctgttt gggcttaatg ccgtagatgc aagcttttga ataccgtttt tatcagaggt
    40141 catgaaaaag ctgtttagca acaagtaacg caatgatttt gaagtgataa ggagatgtgg
    40201 ctgaaaaatg ccgcatctcc ttttttattc ctaaatatga atacgcatta ttttcaatct
    40261 ttttgtagaa aaagggtaca atagaaaaag agaaaggcgg gagaatatgg ttatcaattt
    40321 cattatttta tttttgatga ttgcaattac ttcatttttt gtagcaagtg aatttgcttt
    40381 agtgaaaatc agacgatcaa gattggaaca actagaaaag gaaaatgtaa agaacgccaa
    40441 gctagctctt catgttacac accatttaga taactattta tcagctagtc aattaggaat
    40501 tacgctaacc ggtttgatca tcgggtgggt cggtgaagga tctgtcgcgg ctttactaga
    40561 accagtgatc ggtaaactac cttttagttc ggctattagc agtacaatct ctgtggcgct
    40621 gggatttatt ttagttacgt atatcgatgt ggttattggc gaactgcttc ctaagagcta
    40681 tagtatcgtc aatacggaga aggtcgtctt gttcgtagtt aaaccacttc attattttta
    40741 taaagtaatg tttcctttta tttgggtatt gaatcattca gctgcagggc tagggaagct
    40801 attaggtgtg cgtctcgtat ctgaaggaga agagacattg tctcaagaag agctgacatt
    40861 agtcgcattg aattcttatg aaaaaggaga gttgaccaaa gaagaatatt attatttgga
    40921 aaacgtgttt gaatttgatg ataccttggc aaaagatata caagtagacc gtacgtccat
    40981 gcaagtattc gaagcttcag aaaccgtcaa agaagcgata aaagcttcga ttgaacaagg
    41041 acatactcgc tatccggtta tcttagaatc aaaagacaat gttttaggtt atgtcacctt
    41101 gccagatttg atcaagcaat cttataaaga tgaccagctg acagtagaac agttgattga
    41161 agagccgatc gtaacaactg aaacgatccc tataaaaaaa ttattgacga tcatgcgaaa
    41221 aaagggtaag catattgcta tcttaaaaga tgaatacgga gggacaagcg gcttagtgac
    41281 gattgaagat attttagaag aaatcgtcgg ggaaatccga gatgagacag atttggacga
    41341 agcattgata gcggaacagt cagatggctc ttatatcatc tccggcaaac taacattaga
    41401 tgatttccag cgttattttc atgtagagat tccagagttt gaagaaacga attttactac
    41461 acttgcgggc tttgcttcta gccgatataa agaaataaaa gcaggaacaa tcattgagat
    41521 tgcatcattc cgtttcacgg tattagaata ccagcatgca catatcgatt atttcaaagt
    41581 agagtccacg gaaagaaaaa cagaataaaa gaaaaaaggc tgtgacattt tttgtcacag
    41641 cctttttgtt atctaaaaaa cctgttttcg gtaaacgaag aagatatttt caataaaagt
    41701 tttcattttt agccgcaacg atttctttta atcaaggcta tcttgcttct gcatgttcta
    41761 atgtgaaatg ttcctcttca cccatgtgtt tcaattgttt catcgaacgc atataagagt
    41821 aaagcaacac ggcaacagaa ccgatccaag ctaaaggaga agctgctgct gctcctgggt
    41881 atccccacat tcctgtaaga acgatagctg caaaagaacg cataagcaat tccatcatac
    41941 cggcgatcgt agggatcttg ctttgtccaa gcccttgaag tgtgtagcgt aaaatgaaca
    42001 ggactgctaa gatccagtaa agagacccat tgatattgaa gtaggtttgt gctaattcaa
    42061 atacacgtgt ttctgatggg ttaacaaaca attgaacaaa gaaatgaccg aagaaaatga
    42121 cgatagcacc tgctaagaga ctaaatccga cactcatcag taaagtttga cgtacccctt
    42181 gcaagattcg gccatattct ttcgcaccat agttttgtgc agtgaaagtt gccattgcaa
    42241 taccaaatga catcatcggc tgatttgcaa attggtcgat acgaccagca gcggcttgag
    42301 cagctacgac atctgtccct agactattta gtgctgcttg taacacgata gcaccaacag
    42361 cgatgatcga agattgaaac gccattggca aagcagcatt taggtgtaca gcaaactctt
    42421 ctttttcaaa tttgaaatct tttttgcgta actgtaacaa aggcattttt ttcttgatat
    42481 acactagaca cagtacgctg gaagaaactt gtgcgattac tgtagcaata gctgcacctt
    42541 ccacacccat gccaaaatag ataataaaga tcaaatcaag tacgacgttg atgatgaccg
    42601 caatgatcag aaagaatagt ggtgtgcggc tgtcaccaag tgcgcgaatg acattagaaa
    42661 gcaaattgaa actgactgat gcaaagatcc ctaataaaat aattgaaata aatgtttgtg
    42721 cttgatcaag tatttcagga ggagtttgca tcaattgaag catcggacga ataaacagga
    42781 gacttaggac agttaaaatc acagtgacga caagactaat gacaacacta gcagcaaaac
    42841 ttttcttcag accccgataa tcttttgcac cgtaacgttg tgcggtaata atggctaaac
    42901 cagctgttaa accttgggca aagccaatga tcaaaaatgt caggcttcca gttgcaccaa
    42961 ccgctgccag cgcattttta cctagcgtct gacccacgat gatcatatcg atcatattgt
    43021 agaactgctg aaaaacattt ccgatcagta aaggaacagt gaacatcaaa atcagtttag
    43081 cggggcttcc ttttgttaaa tcgcgcatct tttaaactcc ttatatcttt aaaatttagt
    43141 tagtagtata tagcgtttat cctgagaata ccaattagaa atcaatgata atttatgaaa
    43201 ctccataaaa agaagccaag aattgagtca aagaatcaac aacttggcct ggtctgtaaa
    43261 ataagggaca tccacgttta tctatcacct taatattgtc taatctcact tggtaaaagg
    43321 atcgtttctc tcgtaccgtc atcataggcg tatacttgag caggaaattg gttcgaatag
    43381 gcaaacggca aatcaatatc catttccaca ttgcttcctg cttgagaaaa atgcatcgtc
    43441 acttttccac gattttcgat caaatcaaag gttaatatat tttctaatgg aatgactccc
    43501 ttaagatcta ggtcaataat aaaccatata ctgtcaatca attcatctgg caaacttgat
    43561 acgacaccaa ctgaagcgta acggttgcgg ctgctatcaa aactttcaaa catctgttta
    43621 acctccttcg aatcaacgaa tgttttattt ttataattaa tcctaggata aactagaaat
    43681 acttacttca acgagggttc attctaataa agaatcagga aaaatgcaac aataattgaa
    43741 tacttttttt caaagtataa atttttataa aggtacgata aaagtatgcg aggaaaagct
    43801 tggtaaatta ggatttttcg tttgtaaagc catttacaaa agtggaagcc ccatatgaaa
    43861 ataaacgatt agaaataaaa aaggcgtgtt aaaagctgct gacccgccgc ttctaacacc
    43921 acctgattgt ttcagtatta ttcttctgtt gaaccgatca cttctggttc gccaccttca
    43981 cctgtgcttt cttcttcttc actttgagcg actacagcag cgatttgctc ttcactgtcg
    44041 gtcacgaatt tgtaatcgtc gttttttgga agatcggcta ctgtaataga atcacctaat
    44101 tccaagtttg tcacgtcaac ttcaattcgt tcaggcaatt tgtctggtgt agcagatacg
    44161 actactgtat acaagttttg agctaacact ccaccagatt tagcacccac tgattcgcca
    44221 acaagaacga cttctgtttc gacttctgtt tcttccgaca tgtttacaga tagaaattct
    44281 gcatgttcga aatgattggt aaaagtgttc gtttggatct tgtgaaccaa tgtattgact
    44341 ttttgtccat cgatgtccat cgtgatgact gtgttcgctc catgttcacg caacaatttg
    44401 cttagtgtca aaccatcgaa tgagataggg gtactttcta ctttataccc ataaacaaca
    44461 gctggaactt tcccttcgtg acgaagtttg tttcttaatg aacggggacg tacttctctt
    44521 ttacttactt ctaatgatac tgacataacc aaaattcctc cttaaaatgt gactgtctgc
    44581 tttggtaacg gacagatttt atttaagtga attctaaagg ctaatctcgt gcacatcatc
    44641 gaaggcaggg cgcttaaaaa agcgcaaaag gagagatgcg cgcatctccc cttagaaagt
    44701 caggattcaa cttttctcca ccaggtcatt acgctgtgta tgagaaatcc aattcacttt
    44761 actatttaac cgtaacgtga actgttaatt aagtcaagga ataactatga tgatgtattt
    44821 gcaagcaaga atattcaagg tataattaat tagtagtaat aagaaaggaa acagcatgcg
    44881 tttagataaa ttaatagaag aaaaattaga aacgacacgc aaggaaatga agcggctgtt
    44941 tgctttgaag caagtgatga tagatggcac agtcgaattt cgacaaaatc gaaatgtaga
    45001 tagtttactg catcaaatcg aagtagcagg tgaacactta gaaacaaatg agtcctatta
    45061 tcttctcaat aaacctgaaa aagctgttac agctgtatca gatccagaaa agacgacagt
    45121 aatcgatctg attgcaagtg ccgatcgttt ccaaccatta tatccagtgg gaagactaga
    45181 tagagataca acaggactat tgctgatcac ttcgaatgga caattgggat atgaaatgct
    45241 acttccagaa aaaaaggttt caaagactta tcaagtagtt gtgaacgaaa gggtgacacc
    45301 ggcagacgta acagcatttg aaaatggaat cgtattcgct gatgggtatc aatgtaagcc
    45361 ggctgattta aaaatactca aagcagacaa gcaccaaagt acggttttat tgacgatcca
    45421 agaaggaaaa ttccatcaag tcaaaaagat gttcttagcc caaggaaaga aagtgaccgc
    45481 attgaaacgg ctaagcatgg gaccattgga attagatgaa cagctaggag taggcgagta
    45541 tcgatcgttg acattagcag agatgaaaaa gttaagtatt tattttaaat aggaggtcaa
    45601 aaagatgaaa tataaaactt tattgtttga tgtagatgat acattgttag attttcaatt
    45661 aactgaagcc aatgctttaa gtgctttgtt tgcagaacaa ggaatcacat tgacaccaga
    45721 aatcgaaacc agctataagg ctttgaatca tcatttatgg caggagtttg aaaaaggcag
    45781 actatcacga gaagaaataa taggcagtcg ttttgggtta cttttccagc aatttgggaa
    45841 aacagtagat agtgatcaaa tggaaaaacg ctaccgccac tacttgaacc aaggtcatga
    45901 tcttgttcca ggaagtctag aaatcctgaa aaaggtccat ccgcatgctg atctttatat
    45961 tgtgacgaac ggtgtgtcgc acacgcaaca tcggcgatta acagatgcag gaatgcttga
    46021 ttacttcaag gatatctttg tctcagaagc tgtcggagcg caaaagccga tgaaagaatt
    46081 ttttgatcat gtcttttccc acattcctaa ttttgataaa aagcaaactg tgatcattgg
    46141 tgattctttg acttctgata tcaaaggagg aaatatggca gggatcgata cgatttggtt
    46201 caatagaaat aaattaccag aaatcgaaga aatccagcct acttatcgaa tcgattcatt
    46261 agaggaactt tattcattat tggaaattga aaaggattaa ttttcagaaa aatatagcat
    46321 tttcgtgttt tttcgctata atagattaga atgaaaaaac ggatacgaaa gaggttttag
    46381 tcaatgacaa aaagcaagcc aattatcatt ggtgtgacgg gtggatccgg aagcggaaag
    46441 acaagtgtca gccgtgcgat tttcaacaac ttcccggacc attccatcat gatgcttgaa
    46501 caagattctt attacaagga tcagagccat ttgagttttg aagaacgatt gaacacaaat
    46561 tacgatcacc catttgcctt tgataatgat ctgctgattc agcatgtagg cgacttactg
    46621 aactataaag ccattgaaaa acccgtgtat gactatgtag cgcatacaag aagtcaggca
    46681 acgatcattc aggaaccaaa agaagtgatc attctagaag gaatcctgat tttagaagac
    46741 gaacgtctgc gtgacttgat ggatatcaaa gtatacgtag atacagatga tgacattcgt
    46801 atcattcgtc gtatcaaacg ggatatggaa gaacggggac gcacattgga ctccgtgatc
    46861 gaacaatatt tgacagttgt taaaccgatg tatcatcaat ttatcgaacc aacgaagcgc
    46921 tatgcggata tcattgttcc agaaggcgga gaaaaccacg ttgctattga tttgattaca
    46981 acaaaagtag ctagtttctt aaaccataaa tagtgcgcag cgaaacagtt gaacggactg
    47041 cgaccgtttg aaaagcaagg attctgcttt ttgaacagtc accagccaac tgttttttta
    47101 tttataacaa gaggttgtga aaacagcatc ttgacctaag tattaaaaag aagaaacgga
    47161 aaatagctgt ttatatttta ccgttttttg agctaatacc gaaggtcagc tgattgagca
    47221 ccgtgaagta gaggttgtaa aaaagctgtc tttgcatcaa gcgataagag catccaaata
    47281 aaacagctcc tcacgttttt cgttgggttg ggcttattat cgtagatgca agcttttgaa
    47341 caccgtttaa cgagaggttg taacagaagt ggtcagatgc aataaataag aagaaactta
    47401 cgaaaattat ttctcgaatt tgtgtgagat ttcgacttat ttttttgaag aagctctttt
    47461 gtcgcacccc cta
//
LOCUS       DACDRN010000013        44640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDRN010000013 DACDRN010000000
VERSION     DACDRN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.013"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            366..1166
                     /locus_tag="IWJ80_000963"
     CDS             366..1166
                     /locus_tag="IWJ80_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460716.1"
                     /translation="MSKDLRELKLRKKQYNQYFSSLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSRSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            1203..1385
                     /locus_tag="IWJ80_000964"
     CDS             1203..1385
                     /locus_tag="IWJ80_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460717.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENVEDLRLIGE"
     gene            1575..1862
                     /locus_tag="IWJ80_000965"
     CDS             1575..1862
                     /locus_tag="IWJ80_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460718.1"
                     /translation="MVEVEFGSKEWFEELNSMIKFPEKFTFYYNKRDNIIQVALLETK
                     GRLENTRWSMIKPSALRFHPKDLTAVVAVKEVQDSQEYYEVVKNLKDIWCK"
     gene            1953..2198
                     /locus_tag="IWJ80_000966"
     CDS             1953..2198
                     /locus_tag="IWJ80_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460719.1"
                     /translation="MKEIEGIKKRNQERLELLNENRNRNRMFSYYESMRCLNKSRSEI
                     IESSKYKQLMEERVSLIQEIETESIFPDHSPKVRFYN"
     gene            2326..2655
                     /locus_tag="IWJ80_000967"
     CDS             2326..2655
                     /locus_tag="IWJ80_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460720.1"
                     /translation="MKLEGSLQNRMMEGSTTNKEIVVGMGATELLYTDRNPYTVIEVK
                     SKNRILVQADGAINKATFPDQEWEYSRNPEGQILELVKTKNGWKVLKEDIHFYIGDRE
                     KYYDPSF"
     gene            2708..3539
                     /locus_tag="IWJ80_000968"
                     /pseudo
     CDS             2708..3539
                     /locus_tag="IWJ80_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3554..3871
                     /locus_tag="IWJ80_000969"
     CDS             3554..3871
                     /locus_tag="IWJ80_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460721.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            3874..4106
                     /locus_tag="IWJ80_000970"
                     /pseudo
     CDS             3874..4106
                     /locus_tag="IWJ80_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4221..4697)
                     /locus_tag="IWJ80_000971"
     CDS             complement(4221..4697)
                     /locus_tag="IWJ80_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460722.1"
                     /translation="MTDKTSDLPKLDPKISQVKSKKDGKTKNAVKETSSLLLDIPMSS
                     FDEILDANTDETSVKIASHVGMNSQNDYFYNLLNSLRKLEDKKNQVIASKAPDLTTDL
                     DAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            4823..6154
                     /locus_tag="IWJ80_000972"
     CDS             4823..6154
                     /locus_tag="IWJ80_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP9460723.1"
                     /translation="MVQFTGIAMSVITINPQVLHADGIATYDAQFNCTTEGSGSGTDS
                     DGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRTVEGYY
                     GDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMFKG
                     DGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMKSK
                     GMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEGKI
                     NGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFQGELQGQCTELTWAYMSQL
                     YDGNQPTLGNGGYVWESYKSAGAKVTNKPIVGYAFSALDGWLWAVGSAGHTGVVIAVF
                     DDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            6179..6706
                     /locus_tag="IWJ80_000973"
     CDS             6179..6706
                     /locus_tag="IWJ80_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460724.1"
                     /translation="MNSKTKDAIIISLLSGMLLAMLVGILYFNKQNQDIEAEKHSVQI
                     ENELTFFKEVKPNEVDGLQRDLENDIQEMFKGSDRSKFANQSGEEFLRKLFIVTGKPP
                     VTNESSEQEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            6693..7256
                     /locus_tag="IWJ80_000974"
     CDS             6693..7256
                     /locus_tag="IWJ80_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460725.1"
                     /translation="MQNHEITTAKKAIGILSIILILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            7674..8411
                     /locus_tag="IWJ80_000975"
     CDS             7674..8411
                     /locus_tag="IWJ80_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460726.1"
                     /translation="MADRKLEKLLEETWDPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENRGTWDFWANLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISEQDGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVNITNRLDYDSWADIALEKYIYSTLKEFIENRMNIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(8428..8844)
                     /locus_tag="IWJ80_000976"
     CDS             complement(8428..8844)
                     /locus_tag="IWJ80_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460727.1"
                     /translation="MNKKRKLSVLITLVSMLAILSGILSIKIFNQINTSANTKNEIVI
                     ENTSNSKEISTTKNKDKSDETTTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKDEELYSFVK"
     gene            9237..10484
                     /locus_tag="IWJ80_000977"
     CDS             9237..10484
                     /locus_tag="IWJ80_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460728.1"
                     /translation="MNMKKNSLAMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNINK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            10754..13144
                     /locus_tag="IWJ80_000978"
     CDS             10754..13144
                     /locus_tag="IWJ80_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAP9460729.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDEQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            13193..15028
                     /locus_tag="IWJ80_000979"
     CDS             13193..15028
                     /locus_tag="IWJ80_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9460730.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            15103..15960
                     /locus_tag="IWJ80_000980"
     CDS             15103..15960
                     /locus_tag="IWJ80_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9460731.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKIILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            15950..17029
                     /locus_tag="IWJ80_000981"
     CDS             15950..17029
                     /locus_tag="IWJ80_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9460732.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDGGSFDIGEAEGKVNPYTGDVFSKNQT
                     KDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLPLSGKGHQTVI
                     AGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQNSTLTDIPDL
                     DMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDSEYVSDKTETV
                     TSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTGITNKHQKMLL
                     VIWTVIGALASLNVLILLIKEFRKA"
     gene            17368..18168
                     /locus_tag="IWJ80_000982"
     CDS             17368..18168
                     /locus_tag="IWJ80_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460733.1"
                     /translation="MVGISKTALDSLAKLFSSRFDSAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            18256..19062
                     /locus_tag="IWJ80_000983"
     CDS             18256..19062
                     /locus_tag="IWJ80_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460734.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTAENWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            19293..20162
                     /locus_tag="IWJ80_000984"
     CDS             19293..20162
                     /locus_tag="IWJ80_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP9460735.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGNVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            20182..20508
                     /locus_tag="IWJ80_000985"
     CDS             20182..20508
                     /locus_tag="IWJ80_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460736.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDD"
     gene            20542..21804
                     /locus_tag="IWJ80_000986"
     CDS             20542..21804
                     /locus_tag="IWJ80_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460737.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVANFSKSVYLVGRALVVSAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATRGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETEKSTASIAVSNSQFAFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            21884..23260
                     /locus_tag="IWJ80_000987"
     CDS             21884..23260
                     /locus_tag="IWJ80_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9460738.1"
                     /translation="MAMKLLEKVKNRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISDTPHMLVAGTTGSGKSVLLNEILVTAICHAMPDELKLG
                     IVDPKRVEFGRYKKLPYMLADPITDMDEAYEFFEYLVILMNERYKLMEKAGVQNITQY
                     NKYAEKHGIEKFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMFIKLNGGNKIRAQG
                     AYISNEEIEAIFDHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     VDPEPQTSNNTNNEQPISSKTKPKTTDPKVSVKVTITTDNSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            23257..23886
                     /locus_tag="IWJ80_000988"
     CDS             23257..23886
                     /locus_tag="IWJ80_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460739.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKKDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNERKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            23898..24356
                     /locus_tag="IWJ80_000989"
     CDS             23898..24356
                     /locus_tag="IWJ80_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460740.1"
                     /translation="MLTILKLYREGFNEKVSIGESIEYRGDKYIIIRILKINKHYFSK
                     SISLTVRVLVQKVGDLSDYYNCKREVIVDNRYDQSKDDKRIIKIGDIVFPEKGIAYEV
                     VSINSFRYEFVDLVVEYSVRLIVPWSKEEINEALKDERKSTFKVLEGEKK"
     gene            24353..24832
                     /locus_tag="IWJ80_000990"
     CDS             24353..24832
                     /locus_tag="IWJ80_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460741.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGRERVDYITYS
                     VDNTVRCYEIKVTLSDLKSKAKQTFIGDYNYLVVTSDLWEKMLQDDTLMRKYYKQGVY
                     VFNEKYPSMISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIVEIPE"
     gene            24971..25510
                     /locus_tag="IWJ80_000991"
     CDS             24971..25510
                     /locus_tag="IWJ80_000991"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460742.1"
                     /translation="MNKRRILWLIAGMYLCIAVSGFTICRIINQLKNAIVYLGEIGYL
                     EDLKAGLQIMSWILFVLTVALVILTCENFSTIKKQIISNHNQETVKSGLEVDPEEEVT
                     EQAIKEQEEIDKKEIMSIYLSSGESFFHEIDSLKLSSQEIIDRWQNMSLDGELIVEDS
                     EGWSAYKKDEIVAITILFV"
     gene            26161..26214
                     /locus_tag="IWJ80_000992"
                     /pseudo
     CDS             26161..26214
                     /locus_tag="IWJ80_000992"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26218..26817
                     /locus_tag="IWJ80_000993"
     CDS             26218..26817
                     /locus_tag="IWJ80_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP9460743.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIHFDSKEHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            26833..27309
                     /locus_tag="IWJ80_000994"
     CDS             26833..27309
                     /locus_tag="IWJ80_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460744.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMHE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            27326..28228
                     /locus_tag="IWJ80_000995"
     CDS             27326..28228
                     /locus_tag="IWJ80_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460745.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            28301..28492
                     /locus_tag="IWJ80_000996"
     CDS             28301..28492
                     /locus_tag="IWJ80_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460746.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            28657..29244
                     /locus_tag="IWJ80_000997"
     CDS             28657..29244
                     /locus_tag="IWJ80_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460747.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            29244..30143
                     /locus_tag="IWJ80_000998"
     CDS             29244..30143
                     /locus_tag="IWJ80_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAP9460748.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            30143..31057
                     /locus_tag="IWJ80_000999"
     CDS             30143..31057
                     /locus_tag="IWJ80_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460749.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            31109..31324
                     /locus_tag="IWJ80_001000"
     CDS             31109..31324
                     /locus_tag="IWJ80_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460750.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKKFWLNNFSYKDFIKEKANTLY"
     gene            31514..31768
                     /locus_tag="IWJ80_001001"
     CDS             31514..31768
                     /locus_tag="IWJ80_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HAP9460751.1"
                     /translation="MKKEELIDIFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLNSDDVNFAHDVVGIQNHIDRENKRMGDEFLPRYSSL"
     gene            31947..32990
                     /locus_tag="IWJ80_001002"
     CDS             31947..32990
                     /locus_tag="IWJ80_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAP9460752.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            33163..33387
                     /locus_tag="IWJ80_001003"
     CDS             33163..33387
                     /locus_tag="IWJ80_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460753.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            33390..33647
                     /locus_tag="IWJ80_001004"
     CDS             33390..33647
                     /locus_tag="IWJ80_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460754.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            33749..34102
                     /locus_tag="IWJ80_001005"
     CDS             33749..34102
                     /locus_tag="IWJ80_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460755.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            34155..34949
                     /locus_tag="IWJ80_001006"
     CDS             34155..34949
                     /locus_tag="IWJ80_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460756.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            34978..36096
                     /locus_tag="IWJ80_001007"
     CDS             34978..36096
                     /locus_tag="IWJ80_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460757.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGNSKDEVKGVVIDDDFEFDFD"
     gene            36129..36449
                     /locus_tag="IWJ80_001008"
     CDS             36129..36449
                     /locus_tag="IWJ80_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460758.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            36704..37597
                     /locus_tag="IWJ80_001009"
     CDS             36704..37597
                     /locus_tag="IWJ80_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460759.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EDEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHEELSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNAYI
                     ARLKRFIDKYKENSDKFSKDK"
     gene            37617..39245
                     /locus_tag="IWJ80_001010"
     CDS             37617..39245
                     /locus_tag="IWJ80_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460760.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKEFEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            39296..39664
                     /locus_tag="IWJ80_001011"
                     /pseudo
     CDS             39296..39664
                     /locus_tag="IWJ80_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            39764..39964
                     /locus_tag="IWJ80_001012"
     CDS             39764..39964
                     /locus_tag="IWJ80_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460761.1"
                     /translation="MLKRIAWDKDNKTVIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            40098..41324
                     /locus_tag="IWJ80_001013"
     CDS             40098..41324
                     /locus_tag="IWJ80_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460762.1"
                     /translation="MIFYMFININEVLKMTTITMTKNIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYRNKSKYGLLPFRGRDIKIHKADKFYISLPKGFRLKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            42249..43574
                     /locus_tag="IWJ80_001014"
     CDS             42249..43574
                     /locus_tag="IWJ80_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9460763.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRANDLPYPLPKELYIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSETRSERRRELAKIIKDEIEKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            43567..43914
                     /locus_tag="IWJ80_001015"
     CDS             43567..43914
                     /locus_tag="IWJ80_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460764.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFAELLKWSQI"
     gene            44318..>44640
                     /locus_tag="IWJ80_001016"
     CDS             44318..>44640
                     /locus_tag="IWJ80_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP9460765.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSK"
BASE COUNT        17013 a         5893 c         8995 g        12739 t
ORIGIN      
        1 aagaaaagaa aattattgat attatacaca ttagtgtgta taataaaaga gagggagtga
       61 ttagaacttt gagcaattca ctaattgttt aataatagaa cgattcctaa gaagttcatt
      121 gttcattggc aaattgcttt atcagattga aagttggcga ataaggtttg tagaataaca
      181 ccttttcaag caaagcgatt tttgaaagct atgaaatttg tttaataaaa aaattcacca
      241 gataatctga tagagactaa cccaattact aacagttgta attggtgtta gcttacaaat
      301 taattattgt gtttagtcaa ttacaaattg tgaaacggca ctaataattg gaaaggaaaa
      361 atgaaatgtc taaagattta cgagagctta aattgcgaaa aaagcaatat aatcaatatt
      421 tttctagttt gttgaataaa catattgaag aaggacttga tcatgatttt ggaaaagatg
      481 aatattgttt atattgcgaa cgttacaatg aaaaaatgtc tgaatatcta cagctgaaaa
      541 aagagataca gaaattagag aaaaaagtac atgcttggaa gtataaagat tccaggtctg
      601 gttatgttga atctcaaaat aagccaagaa aaaagaagca agcgtatgtt ctaaagatgg
      661 taaatgggga agagttaatt gtattagcgt acagtgaaga agaggttcga gaactcgttg
      721 aaacacctgt caggaaaaag gttgaaacaa taaatgagat agacgacagc caatacaata
      781 gcatctatat tgaaacaagt catggtggat ataagaaatt agcaactttc cttagaagtt
      841 ctaaagttgg gataatttct aaaaaagaca taagtgctgt taccgatagt ttttgtactt
      901 tatctgttcc taaagcttat ttccaatacg atagtaaaag atttgaacgc ttagagaaat
      961 tctgtagacc gtatactgta ttgaaaacta aaaaatttaa taaaactaaa agagtttcga
     1021 tacagttacc gactggtcaa atctcattag gtgaaaaaga ctatctgata aaagtaggaa
     1081 aagagttaat tataaaagta ccggaacact tatttaaaga aaattttgag atacgtgaaa
     1141 ccttggtatg gaaagaagaa aaatagtttg gaagacatag aatggtataa ggggaaattg
     1201 aaatggcagt aaataaaata ttgcaggtat taatcataga cgaatttggc ggaggagaat
     1261 ggtttgatgt gcagtccata gactttgaag aagaagtcgt agttatagaa aatatttacg
     1321 gcgaagtata ctcatattcg cttgaaggtg agaatgttga ggatctacgg ttgattggtg
     1381 agtagaaaaa agcagaaata tcaaaatgga gataaaaatt aatagcatgt aaagatagtc
     1441 atgcttacat gctttatcca aaatggtatg cagacaaaaa tatagctgaa ataaacgatt
     1501 atctaactag tataaaggaa cgcaatgcaa ttaattaaca atagacgaaa aatgaaatta
     1561 aaggagatag gaaaatggta gaagttgaat ttggatcaaa agaatggttt gaagagttaa
     1621 actcaatgat taagttccca gaaaagttta ctttttacta caataaaaga gacaatatca
     1681 ttcaagtagc tttacttgaa actaagggaa gattagaaaa tactcgatgg agtatgataa
     1741 aaccatcggc acttagattt catcctaaag acttgacagc ggttgtagcg gtaaaagagg
     1801 ttcaagactc acaagaatat tatgaagtag taaaaaatct gaaggatatt tggtgtaaat
     1861 aattctaaat tgaccagtta tttggtcaaa aatagtatgt gattatttca tccaatgtgg
     1921 catcataatt ggtatggaag gggataaatt atttgaaaga aatagaaggt attaagaaaa
     1981 gaaatcagga aagattggaa ctgttaaatg aaaatagaaa tagaaataga atgtttagtt
     2041 attatgaatc tatgaggtgt ttaaataaat ctagaagtga aattatagaa tcatcaaagt
     2101 ataaacaatt gatggaagag agagtttctc tcatccaaga aatagaaaca gagtctattt
     2161 ttccagatca ttctcctaaa gtccgatttt ataattaaac attaacaaat gaaacgatgc
     2221 taaataggca tcgtttttta tctatttata aaattaattt atttacaaaa tgtgtatcgg
     2281 tatgtatggt gaatttgtag ataagttgag aaagggtgat cttaaatgaa attagaaggt
     2341 agcttacaaa atagaatgat ggaaggcagt acaacaaaca aggagattgt agttggaatg
     2401 ggagcaaccg agcttctata taccgacaga aatccttata cagtaattga agtaaaaagc
     2461 aaaaacagaa ttttggttca agcggatgga gcaatcaata aagctacttt tccagatcaa
     2521 gagtgggaat atagcagaaa tcctgaaggt caaatattgg agttagttaa gacaaaaaat
     2581 ggatggaaag tgcttaaaga agatattcat ttttatatag gtgatagaga aaaatactac
     2641 gatcctagtt tttaaacaac ctgaagatga gcaaggtttt atcctcgctc agaaaggagt
     2701 atggaggatg ttcagtcaag aagaaatcat cagcaaatcg caaccggata aaaatggaac
     2761 tataatcatg aagaaaaaaa tcccttgcta tcgttgtagc ggatcaggtc agtacaagtt
     2821 tatgtctatg gggaaaatta cctatggcac atgttttaag tgcgatggtg atggatttct
     2881 tgttgtaact gaaaaatcct ttacgccaga atatagaact aagctaaatg ttttaaataa
     2941 aaaaagagct gatcgaaaaa taaggatcgc aagagagaaa gaatctcaga aaaattttga
     3001 tcgttatggc tttgaaaagg gttatattta cgtggttata gagaaaaata gctatacgat
     3061 caaagatgaa ctgcatcatc aaggagctac ttatgatcct ttattaggtt ggttttttta
     3121 aggaaaaaca agaacaatac aaaactcaaa aagtttttgt agaagaattt tctgtaatca
     3181 atgatgaagg aattattgag ttgaagctag aagaatacag caaaagacaa caggagattt
     3241 taagtgcgaa gcgtagagaa gagggtgagt ttattggtac aatcgctcaa cgattggaat
     3301 tggatttgaa acttaccaat accgtcacca tagatacaca atggggcaat gtttacctgc
     3361 atttatttga agatcaaaac cataattcat tgatttggaa gacaaataaa tttctttatt
     3421 atgatgaatg ggatgttgga gaaataagaa aagttaaggc gacaataaaa gcgcatgact
     3481 actacagaga taccaagcag acttggatta aaaatgttaa aattcaaaaa aatgagtaag
     3541 aagggataaa agtatgaaaa gagaagaata caaacaaaga ttaaacgaac tgcttgaaga
     3601 ggacgaaaca ttaactcatg gttcgccgga tgaaattcta tacatgattg ataatatggt
     3661 aatttttggt ggatatgaac ttggtaatag gtcagttgat cataatattt tagaatttga
     3721 tgatgtatca tgggaagaaa ttcttgattg gggaattttg gcagtacctg aaactaaaac
     3781 ctatattagt gacactatgg ttcctttttt tgaggaattg gattataaaa gacttcccaa
     3841 aaatgaaaat catatactgg gaggaaacta acaatgttaa gtgacttact tattttactg
     3901 atctatctag gtggaactga aattattgcg tattttttgc ttgtaattgg tgaatatggt
     3961 atttccagtg caatcactca aaggtttaga ttaccagtaa gtgaactaaa aaaatgatga
     4021 gattatcagc atatctttta atgtttttga atgtggtcat gttaatcttt gtagtaatcg
     4081 gtgtgacttt caatttaacg gaatagaggg aaatctataa tggaaaaaag agaaaagaat
     4141 gaacatattt tttgagtcaa tattgttgat ggtttttgcg atcagcgctt accagaaatc
     4201 taaaaaagag taatgactgt ttagtcatca ctcttttttt gtagggaaat tgaaatatgt
     4261 ggagcttgta ttggtttagt taggattgct tggctataag gagctagttc tttatcaaaa
     4321 ccatcaaagt aggttttcag tagcgattta actgatcgct catcttgttt gatttggata
     4381 atttcagcat ccaaatcggt tgtgaggtca ggagcttttg aagcgattac ctgatttttt
     4441 ttatcttcca actttcttag gctgtttaac aagttgtaaa aataatcatt ttgactgttc
     4501 attccaacgt gagatgcaat tttaacagaa gtttcatccg tattggcatc aagtatttca
     4561 tcaaaagaac tcattggaat atcaagtagt aacgaggatg tttcctttac agcattttta
     4621 gtttttccat cttttttgct cttaacttga gatatttttg gatctagttt aggtaaatca
     4681 cttgttttat cagtcattta ttagcttttc ctttctatta atttatgtta ttatattatc
     4741 ataataacat aaaaaagtac actttttggg ggcgtagtta ttgaaaaaat ctaagagaaa
     4801 gattgccacg ttgcttacgt taatggtgca gtttacaggg attgcaatga gtgtaatcac
     4861 aataaatccg caagtgttac atgctgatgg gatagctact tatgatgcac aatttaactg
     4921 tactactgaa ggtagtgggt caggaacaga tagcgatgga aacgatactg gaagtggcgc
     4981 tgtaacaggt gatgtagcca aaaatattga agatatttac aaatacatga atggagaata
     5041 tggcttatca ggtgctagta ttgcaggtat tttaggtaac tggaaacaag aatcaggtat
     5101 tgatcctcga acagtagaag ggtattacgg cgatgggcag agagaagttg acttcatcaa
     5161 acaatttact gatggtctaa tgggtattgg ctatggtcaa tggacagccg ggcgacatac
     5221 agcattagtt gattggtcta agaaaaataa tggcggttct tggtggacta cagaagccca
     5281 attggacttt atgttcaaag gtgatggggg attcgtagga atattaaaag attatgcttt
     5341 aaatgcttct gatgatgtag tagaaaatgc agtaactttt cataaaaact gggaaatttc
     5401 agctgattcc gaatcagctg taatagctgt acgtggtgaa aatgcaaaag aaatctggaa
     5461 gtacatgaaa agcaagggta tggacggtgc aaaagatgaa agtaagatca ataaaatatc
     5521 cggaggttca agtaccggag atggatcgaa taattcatca agttcagcat caggaacagt
     5581 agaagatgcc tgtgcaacta cggatgatgc taattctggt tctggatcaa ttgggaaaga
     5641 aggaaaaatt aacggtaaaa aaggtcaagt aatagcaaat atgacttatg aagaagcatt
     5701 aaaaaaatat gggaaagcaa tcacgctacc aaaatttaaa gaaccagact ggtcaacatc
     5761 accttttcaa ggtgaattac aaggacaatg tactgaatta acatgggctt atatgagtca
     5821 attatatgat ggaaatcaac caactttggg aaatggcggt tacgtttggg aaagctacaa
     5881 atcggcagga gcaaaggtga cgaacaaacc aattgtaggt tatgcgttta gtgctttaga
     5941 tggatggtta tgggctgttg gatcagcagg acatacaggt gtagttatag cggtgtttga
     6001 cgatggatca tttttgtgcg ctaatttcaa tgttccacct acccaagcac caacaagagg
     6061 tgtttcagta actttgattg atggtgtgga tgggtcagat aatgtgcatt tctttagcgg
     6121 tgtaggcaaa gctaaatttg aaattgaaaa ataaaaattt gaatggaggg cgacagagat
     6181 gaatagtaaa acgaaagatg caattatcat ttctttgctt tctggtatgc tattagcaat
     6241 gcttgttgga attctatatt tcaataagca gaatcaagac atagaagctg aaaagcacag
     6301 tgtgcaaatt gaaaatgaat tgaccttctt taaagaggtt aaaccaaatg aagtggatgg
     6361 attgcaaaga gatttagaaa atgatattca agagatgttt aaaggatcag accgaagcaa
     6421 atttgcgaat cagtcaggtg aagaattttt gcgaaagtta tttattgtga caggtaagcc
     6481 accagttact aacgagagta gcgagcaaga aaaaatagaa tactataaac cttttacttt
     6541 taagctaaca aactttggaa ttaagacatc tagtagtggg ttgtatgaag cattgactga
     6601 tttcgaagtt caatacaacg gaaaaaaagt gtatgatacc acattaacca tcacactaaa
     6661 taatgataag aaagtgactg ggggtgcttt ttatgcaaaa tcatgaaatt actactgcta
     6721 aaaaggcgat cggaatactt tccataattt tgatcctttg ttgtgtggta ctatttcaaa
     6781 agaaaaatgc ttatgataat aatgtcaaag aattggaaga ccttaaaagt aaacaaatcg
     6841 aagtagtcaa gaataaccag aaagtgaaag ctatgcaaga agcccaatac aaagaattgg
     6901 gaaatgctga aatcaaggaa caaagtagga aattctttaa tttattttac aactggaatt
     6961 catgggaaaa atatacgaag aatatgaagg aaatacaaaa agaatttcct cagatagtaa
     7021 agtctagtcc tgttgatatc tctggcacaa tggtaggtac tggtaatagt ccaatatcta
     7081 gctatactgc tgattactat acaacaaatc aaaaaggtaa aatggttgaa ttgattacac
     7141 agacaagaaa tactccaaca acagcaacaa caacaatttg gcgtggagta acaagcatag
     7201 gagaaaatca attgttttac ttggaggatt tagatagcta tgtaagagca gagtaaaata
     7261 aaaaaagagc taacggtacc aacgttagct ctttgtgctg acgatgataa caaaacctac
     7321 cgaaaaagga ttgtttcatc acaaacacaa acagaaaaga attgataaaa aacccgaacc
     7381 tcagaatttt tattaatcaa ttcattttga ttataataaa ttgagctgaa cattacaata
     7441 cgtatatgtt gtaaaacgta aaaaaagtca aaagtaaata ctttcttaaa aagtcaaata
     7501 aaactattaa agacaatata tatggtttat ttgacataag aaaatggtct agatgataat
     7561 tagaatatag aattgtagac ttaaataaat cataaaaaac cttctatgat aagttatagc
     7621 tcttaatttt aagcagtttt agatataaga aagtaaagtg ggggagaata ataatggcag
     7681 atcgtaaatt agaaaagtta ttagaagaaa cgtgggatcc aaaagaattt agtgaatttt
     7741 ttatggagaa ttttgaaact gacttagcgg tgattgttaa agatgctctt agagagcaag
     7801 ggtatcctga aactgcaaat tatataaaca ttaattttac tttgtatact gaaaatagag
     7861 gaacttggga tttttgggct aacttagcaa ataaggagtt aagtgataaa tcagatacag
     7921 gaattcgtaa cttttttgaa tcaaacagag atgattatat gtacgctaac aatcaaaata
     7981 aattgaactt tagagtagag tttgatgaaa cacctgaaga atttatagaa agacaaccgc
     8041 ctaaagaaaa tgtagcaaag gtattggaag atcgttggaa ttcagatgaa atagtttcaa
     8101 caattagcga acaagacgga caatatgaac cgctagtaga agcggttaga gaagagctaa
     8161 gactgaataa gtttcctgat gttcaaaata ttgatgtttc tcaaattgaa ataaacgtca
     8221 atatcaccaa tagacttgat tatgatagtt gggcagatat agcattagaa aaatatattt
     8281 actcaacgtt aaaagagttt atcgaaaata gaatgaatat catgtatcta cagcatccac
     8341 agtatttaaa ctttggtgtt gaaatagcta cacctcttga agaatggaaa atggagcaag
     8401 ggcttgacta gcctttgctc cattttttta ttttacaaat gaatacagtt cttcgtcttt
     8461 accaagaatg attgtttgcc caactttgag attatcaggt gatccttcag cggtttgtaa
     8521 ggagaacgtt ccattgaact tatcggatac cgttataggt agctttaatt ctgatccttc
     8581 ctcatctttg agaattgctg ttttccgttc atcaaaataa acagtcttta ttagataatt
     8641 tttagtagtg gtttcatcag atttgtcttt gtttttagta gtactaattt ctttagaatt
     8701 agatgtattt tctatgacta tttcgttttt ggtatttgcg cttgtattta tttgattgaa
     8761 aattttaatc gacaagatcc cactaagaat agccaacata gaaacaagtg taattaatac
     8821 tgataatttc ctttttttgt tcatttcttc ttaacttcct tttttgtttt gattagttag
     8881 atattcctta tctaataagc ctttttaggc tattaaatga agataaaccc caaaaaatta
     8941 ttgcaattat gatagtgcaa tggtactatt attataaaat aaattttgcg aaaggggtag
     9001 gtctaaatcg caaataaaaa gaaattgaac ctagacaaat acaacttgta taccgcaagg
     9061 gaagcaagtc taattttggg taaaggtaga aattatgtta caaacataat caactattat
     9121 ccaaatcgtt tgcctaaagg ttcgttcaag aaagtgggtg acaaaaccta tattataacg
     9181 gaagaaggaa tagaagcatt gcgatcacaa acagaaaata aaaacaaaaa gaaggaatga
     9241 atatgaagaa aaattcgctt gctatggttt tatttgcatc agctgtactt gcggggttat
     9301 cagtaccagt aacctcacat gctgatgaat ggactgcaaa cacaccggat caaatccaaa
     9361 ttacaacagg acaatcaagt tatgcgttca aaaaaggaga tactatttgg tcaactgtag
     9421 agaagcttaa aagcttaggt tacaagacta ctgttgctga gtttgccgaa ttgaacaatg
     9481 ttgatttggc aaaaggagaa caacggacta tcccgattgg acgaactttc aacatcaata
     9541 aagatacatt aacagttaag gaagcagatg gcgaagtagt tagtcaaact gttcaaaaag
     9601 ataaacctga tcctgttgaa aatacggagc aagagaacaa aaaatccgag actacaaaag
     9661 aatcggaacc aacagcaaac acttctgata agacagaaac aagtgcttca agtgttgatg
     9721 gagcaagttc atctaataca aataattcta caagtgagaa agaagcatca cagggaacaa
     9781 gtgaaagtag cactacggaa gctgatactg gaacatcaac aaataactca caatcaacat
     9841 caaatgatgc aaatgaatca accagtacat cttctagtga tcaatcacaa acaggttcaa
     9901 gtgatgaaac tgacagttct acttcaccaa gtaaacccga acaaccaact aatgttgtgc
     9961 taggcgttcc atttattagt caaggtaaca caatgctttg cgaaggaaca tctttgctag
    10021 aagcgttaca ttataaaggt gttgcaacaa atcaagattt aatgactttt gttaaatcaa
    10081 tgccactatc tcctgataat aatccaaata atggtttttc tggtgaatgg agacacaatg
    10141 tggatggaac ttatcaaggt atgaatccgg ctcctgtagt ttcatggggt gttgctaatg
    10201 gcggaaatgt tgtagatatt tctggttcat cggtacaagg attaaaagat gagattgtaa
    10261 atggaaatcc tgtagtagcg tgggtaactt atgcctttga acctgctcaa cataaatcaa
    10321 tgttttgggg cgatgctatt tggaatcgac atgtagtagc cgttgatgga tttaaagacg
    10381 gtttttacca tgttgtcgat cctgtgtttg gggcaagttg gattaatgca ggatcatttg
    10441 aaacagcata taacgtatct caattagcgg tttcggtacg ttaataaaag agatcgttta
    10501 tatactaagt agttaagtat agtctttatt tgtccttgct taactactta gaaaacttgt
    10561 tgctcatggt tggtagaact aaaaggggtg atgctatgaa tactagagcg ttgaaacagc
    10621 ttgctttcaa cgaaaaaagt gattaatagg tgtggtgatt ttaacttagg aatgggcgcg
    10681 aaggtcatta ccacaccgac atatcgtggc tcattttctt cgcctaactg gtataaagaa
    10741 ggagtaacga gagatgaagc atgaaaaaac gataatttta aaatcaaaaa taatttattg
    10801 ttttgcgact ttagtaatgg tgttatctac gcttataatg cctttttcag caataagagc
    10861 tgaagcagat gtcacaaaac cgacaagtgc aaaatttaca gaaactggcg taaaaacaga
    10921 cggttatatt agggttaata tgccgaatca tccaaatgaa tggatgattt caagtcaatt
    10981 taaagattct catggaaata ttggatattg tatggattca gaattgccat ccccaacagg
    11041 aagtggagcc ggaagcttga agtacaaggg agctggcagt gatgagttct atcgtatgtt
    11101 taaaggtggt tttccatcaa aaacagcaaa ggaactgggt gcaggaaatg atacagaagc
    11161 atggtatgca acacaattag tttcatgggt tttagccgga aactttaaag tttcacagat
    11221 tgtatggtca catccaaacc ataccgccgc agaaacagct cgtgtaaaaa aagcatttga
    11281 aaaaatttat gactatgcta agaatggaaa agatacgccc aacactgagt tttctatcac
    11341 agcttctaag acagctgacg aaggaaaata tcacactttc acatataaaa cagcatcaaa
    11401 taagactggt aatgcgaagc tcacttttac atcagcaaaa ccagctggta tgaagattta
    11461 tgatgcagat ggtaaagaaa ttactaacaa cactgtgaaa ttaaattcaa gttttactat
    11521 taaagtacct gtgactacac caagtggtac tttgtcattt aaaggtactg ctaatgttag
    11581 cacaaccaat ccttttactt ttgatggacg aggtgtgtat caagatgccg tagttatgat
    11641 cactacttca gaaacaaagg actctaaatc tttgagtgct aaatggacaa gagctgttgg
    11701 tcaggtaaca gttaagaaag ttgatcaaga tggaaatgct ttaaaaggag cggagtttac
    11761 tctaactgac aataacaatg ctaaatctgt taagacaacc ggagatgatg ggttagctac
    11821 gtttgatttg caacaaggct taacatacag cttagaagaa acaaaagttc ctgaaggtta
    11881 tcaaggttca tttaaaaagg atggtattac tgttaatacc gatggacaag aatttagtta
    11941 cactgctacg aacacaatta aaaaaggtaa agtaaaagtt aaaaaggttg accaagatgg
    12001 aaaaccatta gctaatgctg aatttacctt gacggatgat tcatcccaaa cacaagcaga
    12061 aaaaacagat gaaaacggac ttgcaagctt cgatattgta gcaaacaaga cctacagcct
    12121 tgaagagacc ggagtaccaa ctggttatac aggcgtattc aagcaagaag gaattaccgt
    12181 taaagatgat ggacaaactt ttgaatatac agctacaaac actttgaaaa aaggtttagt
    12241 aaaagttcat aaagtcgatc aagatggaaa acctttggca aatgcagagt tcactttaac
    12301 cgatgaaaaa ggtgcaacac aagtagaaaa aacagatgaa aatggacttg caagcttcaa
    12361 tattgtagca aacaagacgt atagcctaga agaaactgga gtaccagtag gttacacagg
    12421 tgcatttaag caagagggta tcaccgttaa agacgatggg caaacttttg aatatacagc
    12481 tacaaataat ttgaaaaaag gtgtagtaaa agttcataaa gtcgatcaag atggaaaacc
    12541 tttagcagat gcagaattca ccttaacgga tgaaaaaggt gtaaaagaag ttaaaaagac
    12601 ggatgcaact ggtctggcta cgttcaacat tattgcgaac aaaacttaca acttagaaga
    12661 aacaaaagta ccaacaggtt acacaggcgc attcaaacaa gaaggaatta ccgtcaaaga
    12721 tgatggacaa gtctttgaat atactgctaa aaacactttg aaaaaaggtg tgatcaaagt
    12781 tcataaagtg gatgatcaag ggaaagcatt agcaaacgca gaattcactt tgacagacga
    12841 caaaggtgta aaagttgtac aaaaaacgga cagcaatggc ttggcaacat ttaatattgt
    12901 tgccaacaaa acgtacaaaa tcgaagaaac acaaaaccct aatggttata cagggaaatg
    12961 gtctaaagaa ggcatcaccg ttgctgacga tgaacaaacc tttgaatata cagtaattaa
    13021 tcaccttgta cctgaaacag taagaacagg cggaatcggt acatcagcct tccttcttgt
    13081 atttctaatt gcaggattga gtatggtgct actgtattat cgagaacagc acaaagcaaa
    13141 ataaaaaagt gttaatggaa aagaagtaaa tatgaaaaaa gaaggctgac gtatgaaatc
    13201 aaaaaaaatt ttttcaaatt taatgctatt tggtactttg ctaggagtgg gattgggcgc
    13261 aagctcaatc tcttctagcc ttggactagg ggggcaagta gcatatgcac aacaaaacta
    13321 taattatcaa ggaactggaa cgattcatat ccataaagta acaaatacta atggggatgg
    13381 gaaccctgtc tcagatattg ataacaaagg gaaagaaata gctttaccaa atggtgtaaa
    13441 acctcttgaa ggcgttgagt tctcttatgt gaaacttact aaagatgaag ctaaatcctt
    13501 gggatggacg gatagtgata ctacaaaagg tcaacatatt gatgatgcta aagcacgatt
    13561 attactaaac aaaaaataca gtgaagtaag tagaatgaag actgattcac aagggaatat
    13621 agacatacct atacaagtaa atacagacga tttaacagat aacttgtatt tgattgttga
    13681 aacaggaaca gtcaaaggtg tagttgaaga agcgtatcct atgattgtta acgtacctga
    13741 actaaacgga tcagatgatc aatactttaa ttatgatgta catgtctatc ctaaaaacta
    13801 tttccaatta aactcagcag agcttgataa aaaagacgaa gatggaaagg ctcttgccgg
    13861 aactgttttt gaattgcgaa aagatgatgg atctgatcag gttgttggaa cttataaaac
    13921 agacagcaca ggtaaattaa ctgtagatgg tttggaatta ggtgactatt atttcctaga
    13981 aaccgacttg ggaacagatg ctaataaaac aacctattta ctagatggta aaaaattcaa
    14041 atttagcgtt aaacgtgggg agactgctaa agtagttctt gatgttacca actacaaacg
    14101 acctgtaatt actaaggtaa ttgataaaga aaccgttgat actggcgaag atgttcaata
    14161 tacattgaaa ccatcaattc ctggtaatat tggtaattac gaaaaatatg ttgtcaaaga
    14221 tgttctaggt gcagagttaa cttatgattc atcagaagtg atcgaaggaa tgactttaga
    14281 tactgattac agtatcgaac gtgacggaca aacgacaaac tatgtcttta ctgaatcagg
    14341 tatggataag cttcagaaac aatttgatgc aggggatcgc ttctttacaa ttttgatgaa
    14401 cgctcatact aatagtaaag ctaaaatgga tacaccactt aagaatgatg tttcaacgat
    14461 ctacaacaat ggatatgaac ccgaagacga aacaccagtt gatcacgctt atacaatcat
    14521 tggtgatcac caattcatta aaacagatgg agatacaagc aaagcattga gtggagcaaa
    14581 atttgtagtg aagaacaaaa ttgatccgaa taaagttact tacctaaaac aagatccaac
    14641 aacgaaagct gttacttgga ttgcagataa atctaaagct actgaattcg tttccggatc
    14701 agacggtaag ttccaaattg aaggcttgca atatacctat gatgtagatt ttgataaaca
    14761 aggtaatcca acaatcacag ataaaaaaat caatgattat gctcttgaag aagttgttgc
    14821 tccttcagga tatacaccaa ctagaaatga tgttccattt gtggtatcga atgatgatgc
    14881 ttctagcgaa gtaacagaca tcaagaacct taaaacacct gaaacagttc gtacaggtgg
    14941 aattggaaca gtagtatttc tagtggttgc aggtgtggca ggaacagggc tatatgttgt
    15001 ctacaaacga gaacaacaca aagcttaatg aaataaaatt ccctagagtg tttttggctc
    15061 tagggtttct ttttaaaaag tgtgtgaaaa gtaggtatga gcatgaaaaa gaagaatatc
    15121 aagttgatca tcttaaaaat tgggatgttg ctatgtttag gagtaggtct atatcctgta
    15181 attactcagg cttataccta ctaccaaact caacaaacac taaactccta taaggaaaaa
    15241 tatgatgaag cttctgaaaa ggagaatgaa gaagtcttca agagatattc agaagttaat
    15301 aggaaacgaa aagacacaga acaaacctca gtcacgattc ctgaagataa taacaatatt
    15361 ccgtcaaatg gaaatcttca gaaggataaa ttaggtgaat tagtgggtga aattcttata
    15421 ccaaagatta atgtggattt gccaatttat gatggtgtaa acgattttca actatcagca
    15481 ggagcaggaa tattaaccgg aactgattat ccaactggtg ggattggaaa ccattctgtt
    15541 atatcagctc attcgggcat tcctaataaa aaatatttta cagatttaga gttgctcaaa
    15601 cttggtgata aattttttat tgatatttca gggaaaaagc ttgcttatca agtagatcgg
    15661 ataaaaataa tcctaccaac cgaatttgag gatttgcttc ctgtggcagg aaaagattat
    15721 gtaacactta tgacatgtac acctgttgga gataattctc ataggttact cgttagaggg
    15781 gttagcatac cttaccaaaa aagtattgtt gagaaagaaa agaagaaaga agatttaaag
    15841 gtaaaggcgc agatgaactg gtatcctatc gtagttgtag gggttttatt aataatagct
    15901 gtgatagtca ttattaaaaa aatagtagca aagagacgag gaatgaaaaa tgaagaataa
    15961 aatactactt tttttgactc ttatttgcac tttggtttta acaatttatc ctgcaaatca
    16021 gctctatcat caattcaaaa caaaagagaa tgtggcggaa atccaagaga aaattgagaa
    16081 aaaacaaact ccttcaaaag aggaagtggc aaaaaaagtc gaacaaataa aatcgaacag
    16141 cggtaaccaa ggattagaca atactcagaa aacagacggc ggatcatttg atattggtga
    16201 agctgaagga aaggtcaatc cttacacagg agatgttttt tcaaaaaatc aaacgaaaga
    16261 ttttgaacca tctacctatt caggggcggt tggttatgtc tatattccaa aaataaatgt
    16321 gctagagctt ttgtttctag gcgcttcaca ggaacattta tatagaggtg tggcacagat
    16381 gagtgctact agtctacctt tatcaggtaa aggtcatcaa acagtaatag caggtcatcg
    16441 tggcggttat acgtcaccac aatttcttta tgttgatcaa ttagtagccg gagaccacat
    16501 ttatatagat tatcttggtg aaaaagcagt ttatacggtt ttccgaacag acgttattag
    16561 aaattacggt cagaattcaa cactgactga tattcctgat ttagatatgt taacacttta
    16621 tacttgtcat ccatatcctc agaactacga tcgtttatta gttagaagtc taagagatga
    16681 aaaagcaaaa tacatcacgc cacaagtaac agttggcaat accgcatacg ctgatgccat
    16741 tgatagcgag tacgtttcag ataagacaga aactgttaca tcttctaatg atttatttga
    16801 ggatgttgat cgtgccaaca aggaattaga tggaaataat gatcttaata ttccggaaca
    16861 aggtagcgta gagatgcaag acagaacatt tgctgaaaag gcaagttcaa tattcacagg
    16921 aattaccaat aaacatcaga aaatgctttt agtcatttgg actgtaatag gagcattagc
    16981 aagtctaaat gttcttattc ttttaatcaa agagtttcga aaagcataat gaagattata
    17041 aaaattgata gagtacagta aaaagggatt gatactatta ttgatctctt ttttattttg
    17101 aatagagcta gattcacttt taaataaaat caaaactgat cttaattcta aagaaaaagt
    17161 caagaaaaaa atacaacaaa tttttttatc agaatattta tcagaatatt attagaaaag
    17221 ataaactcaa aagcttaatt taatagggtt attggttgta tttagaaata atgtatggaa
    17281 aagctataaa gaaatagata agatgtagaa aatcttattt gtgaatgaaa aacaagtcat
    17341 cgtaaaatat taataggagg tgtagtaatg gtgggaatat caaaaacagc tttagactca
    17401 ttagctaaac tatttagtag caggtttgat agtgcagaat ttgttgaata tctaagccaa
    17461 aacgaaagtg gaaaaactct gatcgaaaca atgaaaaaag actatttgaa tgaaaatgac
    17521 tttccgacat ggacgaatat tgaagatgct acatgggatg tagtgtgtac gatatattct
    17581 ccttgggtaa cgattgctga ttttgtttta gataaaattg gaggggatac ataccctgat
    17641 gccaaatctc ttgctacaag tgactttaaa acgttctatg gttatctaat aggtctaata
    17701 ggcaatggaa tagatttgaa agttacgcca aaacaaacac ctgaagaaat tgaaaaagaa
    17761 aaaggagagg ttcaaatggc ttatgcaaat gacagagaaa aagtaaaatt attggaaggt
    17821 aaattatctg aagaagaact tggaaaaatg gtgcaagaaa tcaatgaaga cagcaactat
    17881 tttcctgaat tgaaggtata tcaaaatgac gaagatttct ttaatgaaat gtttagtaat
    17941 agtccatggg aagcagtgag taaaacaaat ttaggagaat ggaactttca agatgatcta
    18001 gttgcattta gcaatgatgg gtatttacga tcatattcag aaagtgagta tagagctgaa
    18061 ttaaaaacgt atgaaacgga tattctgatg agctatttaa atctattaga gagtggtcag
    18121 ggagagttaa gagataaagt tgaagaacta ttaaaagaag agctataggg attatttcaa
    18181 cataaacaca agcagaaaac taccaaacaa aacgtaccat gcaaaaagtc ggttaagccg
    18241 gctttttgct ctattatgag aggagatcaa accatgagtg caacaaccga tatggcttta
    18301 gaagcagtaa taaaagataa tacaggaaat gtcgaaaatg tagaagactt tagcgatatt
    18361 gcaatcaatc tttcagcatg gatttcagct gtaggagaag aaaatatcga atttgagatc
    18421 aagaatatgg atgacttaaa agcaaaattt ccttttatcg cttcgaaact aagtcaattt
    18481 gccgaggata aatcgtggaa tgaattagtt agtttttccg aaaaaattta tgatattacg
    18541 caagaagatg agtgtgtttc atgggaaatg aataatcaat acaagaaaat tgatgctttc
    18601 gtcaaatcac gtatagaagg aacagctttt gagtttgaag gtatagatac cgcagaaaat
    18661 tggttaaata aagaatcctt tattaaaagt atagcggatg atcttgaaaa acaatggtct
    18721 aaggaagact atgcagagta cctaaaaaca gaaagagata cagaggtgaa agaaaaactc
    18781 actgaggtat gtttggatga acatattgat cccaatatcg taaaacaaga agactatgaa
    18841 gtggttattg ataaagttcc tactgatttt gaggaagttg taaagtatat agttgaagaa
    18901 gcattatcgc aatactcttc agatgaggaa gtggacgaag aaactgttcg taccagagtt
    18961 tggcaactgg tttttgatga caaagattat aaatatttga gtgatcaaaa ctcggttaat
    19021 tggagtgtac gattattaat tgatcaaaat gatattgaat aaaatagagc tgaaaagctc
    19081 tatttttttg ataaaaaaac tagctatttt attaaaaata atttggttca aaatttattt
    19141 ttttaacttg ataatttttt gtcatccttt tttaaattgg ctatatatca aggtttatag
    19201 tgggaacaaa taaaaataca tttgacaaaa tttacaatat agtttattat atatttatct
    19261 tattatgata acacaataaa aggaatgatg gtatgaaaaa gaagaagttt agtcgatcgt
    19321 tgttaggaat tttggtttta gttgcggttg gtcttagtgg caagggttac tttcagaata
    19381 acagcactca ggaacatgat tttcagatca aaagtagtgt tcaggtaggt aataaagcag
    19441 acagtgaatc aaaagaattt ttagggatac gttatgatcc aaacaaatat ccaaataata
    19501 tcgtaaacat taatgatggg aaatatacac tgacaaaaga agaccatgaa gcattatcag
    19561 aaaaaggcag tcaaaccttt tgggtaaatt atgcagattt agatcaactt ggaagagcag
    19621 gaaatgttga agcgttgatc acctatgatg ctgtaaattc tcactcatct aaaaatgtgg
    19681 aacgtccatc tttcaaaagt tctgtccata ttgcaggaga atacaaggat ggagtatttg
    19741 atcaagagag agatacttgg gtcggcaaag attcaaacaa tgaaatcact cagctagaag
    19801 gctataaagg ttatctctat aacaaaagtc attcattagc atggtcgcta ggaggagata
    19861 tggaggtaca caatttaaca cttggaacga gagcgcaaaa tgttgggaaa aatgatcaga
    19921 atggagggat ggcttactca gaaacgattg tacgtgatgc aatctataaa aataagaatg
    19981 aaaaagtatt gtataaagtt gatccaattt ataaagggaa tgaattggtt cctcgtggtt
    20041 ctcatgtcga agctttttca gttaacgata atggaaaaac ggtaaacctt aatgtttatg
    20101 tatttaacaa tcaaaaagga atcaacatca actatcaaaa tggtacttgg gtaagagatt
    20161 agaaattgag aaggtgaaga attggaaaca ataagcatca atggattaga taaatcagtt
    20221 gcaaatcgaa taaaaaaagg aaatggctac tatcttctga agaagggtaa atattcagtt
    20281 gctagagagt tagaaacaat gagtatcgtg tatgtagcta agaatcattt tgacgtagaa
    20341 caggctattt ttgatccaga ttctgaaata gggaaatgtg ttgaattatc agagaaaact
    20401 gttagtacaa aaggggcgat gatcattgag agagaaatgt tagacgaaat cagagtaatc
    20461 attcctaaag atacgcagat gtgtgaggac agcataagtg acgattaacc aaaatcagaa
    20521 agaaaataag gagatattca gatgagcgaa aaatccattg aattaaagga atataagcga
    20581 agcgatggag aaaaatatta tcctgtagcc aatttttcta aatcagtata tctggttgga
    20641 cgtgcattgg tagttagtgc aattggcata tatattttgc attgggtaac aaaggttgta
    20701 caactgcttg tagatacctt agtaagtatt ctcgtctcgt taaatgatgt taacggaaca
    20761 gatgatttag gattaaaaga gatgtttaaa gagttactca atgcgttttt tccaaattat
    20821 ttaagttatt catataatgt acaaagcttt tttgcaaagc tacacttggt agatatctct
    20881 attttagtat tagaagcagg aacggtattc tttgtcggat atttggttac tttgatgttc
    20941 aggcatcact atggagaaca aagaccattc tttgatgata tggaaagtag aaggttgaaa
    21001 agagaagctc tagaagctat ggatgtgaag tatgatccaa acaagattat aggagatgaa
    21061 acgaacaaca aaaaaagaaa acgaaacaaa acgcaattag aatccgatgt gctatcaacg
    21121 cttaaaaata tcaaaatttc tgttcataca agacgtgatc taagtgtcaa tgaagatatt
    21181 cgagaatata tcgtaaaaat accgcaacca aaagggaaga aagttagaga aacagtttta
    21241 cgacaaattc aagatttagg gtcgatcctg acaagagcaa caagagggcg agctaaattt
    21301 ggggaaaaag aagacgaagt ttcaggtgat tacttcatct tcagagcatc agtaaacatt
    21361 gaaagagaac gtaaggaaag agtaagtacc aaaaacaagg aaacggaaaa atcaacagca
    21421 tccattgcag tttcaaattc acaatttgct tttccaatca atctctttgc agataactcc
    21481 aaaaatatcc aaaaccaaac cgaaaaagct agaaaatatg ccgaaactca aaagcaatct
    21541 ttagaacttt atctatcttc tgctgacgta cctgcaagtc taacagatat gcacgttggg
    21601 aatacatcta ttgaatattt gtttacttta tctcgtacca gaaaaattca actaagctca
    21661 atgaaagaag aaattgaaac ttacttgcat atatcaggtg taatcgtgcg gatcaacgca
    21721 gggcgtattc aaattacttt accattagga gaaaatgatc gtattccaat tgatgtacca
    21781 tcaatgatca atagcgtgtt ttagaagaca tcacaaatag aaacagaaaa tacaaactac
    21841 cagttcacaa acagaaaagt accgagaaaa gagaggaata gaaatggcta tgaaattgtt
    21901 agagaaagtc aaaaatagag ccgaaaaagc tgaatctatt ttgctagata aagaacctaa
    21961 tataaagctt aatggaaaat tcagtgtcaa tatgttgaac acaaaggcta aagacccgac
    22021 agatgctatt tttggaatta agcctgatgg cacaattgct catttttgca tcagcgatac
    22081 gccacacatg ttagttgccg gaacaacagg atcagggaaa tcggtattac tgaatgaaat
    22141 tcttgttaca gctatctgtc atgcaatgcc tgatgaatta aagctaggga tagtagaccc
    22201 aaaacgagta gagtttgggc gttataaaaa attaccctat atgcttgctg atccaattac
    22261 tgatatggat gaagcttatg aattttttga ataccttgtg atcttaatga atgaacgcta
    22321 caaattgatg gaaaaagcag gcgttcaaaa tatcacacaa tataacaaat acgctgaaaa
    22381 acatggaata gagaaatttc cctatgtgat cttattggtt gatgaatatt cgcaattagt
    22441 tggaacacac aaagaagttg aagggctgat cgttcaatta ggacaaatgg caagggcggc
    22501 aggaattcac gttattcttg caacacaatc acctcgatct acagtcgtga caggtattat
    22561 taaagcaaat ttcccatcta gagtctgctt gatggtagca agtgatttag aaagtaggat
    22621 cgtattagat gagggtggtg gagaaagcct aagtccaaag ggagatatgt ttatcaagtt
    22681 gaatggagga aacaaaatca gagcgcaagg agcctatatc agcaacgagg aaattgaagc
    22741 gatctttgat caccttagaa atactatgcc agaaccagaa tatgttgatt accgagcaat
    22801 cgtagctgaa tctcgtggtg agggcggtga agacgaggat tttggtggaa ttggtaaagc
    22861 tcctgtaaga aaatctgcta aaagaaattt gaacacacct ttagcaaatt catcatctaa
    22921 agagccatca attgaaaaga agagtacgtt tgctctaaaa cttgaaaaaa acaaagaaga
    22981 aaaagaaaaa caaggagcta aaacactaga aaaacttcaa gccgaaaaat tagaaaaagt
    23041 tttaaaagca ttagataaag ttgatcctga accgcaaacc tcaaacaaca cgaataatga
    23101 acagccgatt tcatctaaaa ctaaaccgaa aactactgat ccgaaagttt cagtaaaagt
    23161 gaccatcaca accgataatt ctgagaagaa acaagtaaac ggaactactt cacctatcat
    23221 gcaaaaaatg ttaaaagcca aaaaacaggt gcataaatga tcattgtcag ttcttttttg
    23281 aggtggctta cgccatataa atatacagat gaagtagttg tgaaacgagc taagaaaaaa
    23341 gataaaaaag cggtgttaac tcctaaacag atgcttgatc atggaatttt gggtgtgaag
    23401 gttttagaag tgaataaacc gtttgaatca aatagtgcaa gagaaaagtt gcttggcgct
    23461 gaagcagatg tggcatattt ttatcatgac aaaagcaagc atcaaatagc tattaaattc
    23521 aatccaaaat cggcatggcg gtatcaagca agagatgact tgaaacgtag gaaacttaga
    23581 ccaatgctct atccagaagg aaaagaagca tttgatagaa cccacatcgt tcccatcggt
    23641 tttcatggta cagaatcaga taatcgccta ttagtaggtt ggaacagcaa tcaaaataga
    23701 gttcagatca ataaatttga gaagaaagta agaaaattca acgaacgaaa aacaatccta
    23761 tggtttgcag atatttgtaa acagccggac ggaacagcaa agtgggaagc ggtgatattt
    23821 gacgaaaatg gtaaaaaagt aatgtctgaa acatggcatg ataaagctaa atttgtttgg
    23881 cagtagaggt gaaaattgtg ttaactattc taaaactata tagagaggga tttaacgaaa
    23941 aagtaagtat tggtgaatcc attgaatata gaggtgataa gtatattatt attcgcattc
    24001 ttaaaattaa caaacattat tttagcaaat ctattagttt aacagttaga gttctagttc
    24061 agaaagtcgg agacttgagt gattattata actgcaaaag agaagttatt gtggataatc
    24121 gatacgacca atcaaaagat gataagcgaa taataaaaat aggagatatt gtctttcctg
    24181 agaaagggat agcatatgaa gtagtgtcta tcaatagttt tcgctatgaa tttgttgatt
    24241 tggttgtcga atattctgtt cgattgatcg ttccgtggag taaagaagaa attaatgaag
    24301 cattaaaaga tgaacgaaaa agtacattca aagttttaga aggtgaaaaa aagtgaaaac
    24361 agaccttact agacaagctg agaagtgttt atggcatgaa acaaatcgca tgggtgtatt
    24421 tggttgtttt gaagtaacta tcggttggga tggcagagaa agagtagatt atataactta
    24481 ttcagtagat aacacagttc gatgttatga gatcaaagtt actttatccg atttgaaaag
    24541 caaggcgaaa cagacattta tcggcgatta taactattta gtcgttacta gtgatttgtg
    24601 ggagaaaatg ctacaagacg acacactcat gcgtaaatat tataaacaag gcgtatacgt
    24661 gtttaatgaa aaatatccta gcatgataag tgttaagaaa gcaaggaaac aaaaagtttc
    24721 gctaggtaca agatcaactg tattagaaag catggttaga tcgatgaatc gtgaagttaa
    24781 gaaattatat aaagtaaaac cattttggga gatagtagag ataccggaat gatgagtaag
    24841 gaatattata tctattcata tatagtatta actgattatg aggaggatac agcaaatgaa
    24901 aaattatgat ccgaatatgc gatggggtac acatactctt aaagtgtctt ttcaacggag
    24961 gataagctaa atgaataaaa gaagaatact gtggcttatt gcaggaatgt atctttgcat
    25021 agcagtatct gggtttacta tttgtcggat aataaatcag ttaaaaaacg cgattgttta
    25081 tctaggagaa attggttatc tagaagattt aaaggcagga ctacaaataa tgagttggat
    25141 tttattcgtt ctgacagttg cgttagttat attaacttgt gaaaattttt caacaataaa
    25201 aaaacaaata atatctaacc ataatcaaga gactgttaaa tcaggtctag aagtagaccc
    25261 agaagaggaa gtaactgaac aagcgatcaa agagcaggaa gaaatagaca aaaaagaaat
    25321 aatgagtatt tatttatcta gtggagagtc attttttcat gaaatagatt cgctgaaact
    25381 tagtagtcaa gaaataattg atagatggca aaatatgtct ttagatgggg agttaatcgt
    25441 tgaggatagt gaaggttggt cagcttataa gaaggacgag attgtagcta tcacgatttt
    25501 gtttgtatag taatatatac cactagataa tctgaatatg ttgatatgct agtgacgtac
    25561 caaatgtgtt caatagagcc gtggtcacag gcagaaattg ataaaatagt aaaatttaac
    25621 cgattatcaa aatttaacgt tttaggaaat accttttaaa aatcacaata cacaaacaga
    25681 aaaataccaa aaaattaaca atgaacgtag gtaagaacat gacaataaat gataataacc
    25741 tcaaaaaata taatgctctt gtccaacgct atatcgaaaa caaaataacg atgcaagagg
    25801 tcgctgatga attaggcaaa tcaaaaagcc tagtatcaca cgactttaag cacaatgttt
    25861 ttccgaaccc gattgagctg aggaaaaaat ttcaaaagag agtttatcaa gaaataggga
    25921 agtggattga tgaagggaat gcaattgacc ctttaattga aaaatatcct tttttaaaaa
    25981 gcaaaaataa aattcaaagg ttaattcgtc aaggcgatat tcaagctaaa gaacctgttt
    26041 tgacaaccaa tgatttggct aaaataaatc ttcataaaca aattattgaa gactttgaaa
    26101 aaaatctttc aacaactgaa attttaaaaa aatacaatat tccgagagga aagctaacgt
    26161 cgactctttt tagagaatat ggagaatcgg cagttactaa agcaaaggaa gtagcgaatg
    26221 agagaaaaaa acaaaaatag tagtcgatac ttagtagcga ctgtagtttt tgtttgtgta
    26281 ttgattttcc tgtttgattt tttacagatt ggaaaaagta taattggaaa tacattgagt
    26341 gcaaaggaaa tggttgcttt aggtggtttg tatgaaggac atttgttccc gaatatattc
    26401 atttctatcg tttctcatat taacttgtac catttaattt ctaatttatt gttcttagta
    26461 gttcttgaag aagcactgaa tgatcagtat gagtggttaa cattattgat cgttttaatc
    26521 atatcaggat ttctaggaag cttagttcct tttctgctac aaagcaacat cgtaagtatg
    26581 gggcttagtg gaggtatttt tggatggatt gctttatttg cgattgcata catccatttt
    26641 gatagcaagg aacatttatt actaagtgtt tttgtattat tgataatcgg cattttattg
    26701 gtatctactt ttgaatcagg cgaggcaaat gtcttgtcac atgtaatcgg atttgtctgt
    26761 ggaatgatct tgggatttat tctaaagaac gtttcattga tcaaacaaaa gaattaaaag
    26821 ggagtgctaa aaatgccaaa aagaaaagaa aagaaaattc cagtgttttt gtctgtctca
    26881 gaaatgttat cacttcaaat actgttaaga aaaaagattg aaacaataga cgaagaagtt
    26941 agaaataaaa gggctatgca cgaacaacgt gaagcgctaa gagaacgaat ccactatttt
    27001 gatgatatac acactaaaat ctatgaagca cgtacaaatc acgaagacga aagtaaatta
    27061 ggattagctc aacaacggat acttgaatgg cttaatacga atactgatca agaaccgtta
    27121 agagtgatta gtaatctttt tgaatttgga acgaagcatt ggtttaatgc tgtagagaag
    27181 gatgctgact tacaagaagc gtttgatacg ctaagtaagg atagcaaatc catgcgagat
    27241 ttattgatta cttatctaaa tgagcatgag tttgaacaaa cgaaaaccaa aaagaaaaat
    27301 aaagaataga aagttgtgat gaatcatgct attaaccctg ttaggttatt tatgtttaat
    27361 tcttgctttt tttggggcaa tcgctataat tattggtcaa tttcaaacaa ataatccgat
    27421 tatgcgtaac ataggcattg gaattgtgat tgtaaccttt attgtgttta atgccactaa
    27481 gatttttgga agtaacgata aacaatttgg tatatcggga gttaataata gttatgctcc
    27541 agattctttg ggaagggtag taatagatgg aacatttgaa aatgtaacga gttataaaaa
    27601 tgtttcgctg acagtgaagg aagatggcaa gaaagcatca gacgacgttc tcgctactat
    27661 taagccgtct caatcacaat ttgaagtgtt ttatacagtt aaagagaata gccctgtaca
    27721 caagctgaca ttggttttta acgttgatgg agaagaatac tataaagatg taaaaataaa
    27781 taaaacatca acgaaaaaga ttatcgaatc agcgaaaaaa gaagtagcta cagaagaaag
    27841 tacaactgaa tcaagttcta ctgtcgaagc ggaactacca gataaaattg attttgatcc
    27901 tgaaaaagtc aaatcgtttg aatctgtttc tggttctatg gctgatcggt ttaacactga
    27961 agtcttcaaa gctatcaaaa ctgttaaatt ggatactgaa aagcaaatga ttaatgttac
    28021 cttattcaat tcagttgaat ctatggatca ggataaaaaa gttacggttg caagagaact
    28081 aggaaaaaga gtaattggtt tgtatcgtgg tactgctaaa ccagattcag acggtgcaga
    28141 tttgccagtt catgtagttt atgaagacgg taaagttttt ggagattcta cagcaaacga
    28201 agcattaacg attaaattgg ctaaatagcc agaaagttat aataaacaaa tggtgtcaca
    28261 ccaataatat ataggtgtga caccttggag atgatagctg atggtagtaa ataaagaaaa
    28321 agttagaaag tatgtgactt taaaaatagc ttctgttaaa tatttagaaa agcgagctaa
    28381 agaagaagaa agagcaagag gtaagaaagt aacagtttca gagttgttag aagaattgat
    28441 cgagaatgat caaacaataa gacgtgcatt tactaatagg tctatcagat aacaatataa
    28501 ttaataaaaa aagaaagagt gatttggtct tagactaaat cactcttttt atataaagtt
    28561 tctttacact cattccgaat aatgttaaaa ttctttatac gatatatagt tccttgtcag
    28621 aggaagagat aaccagcaat attggaggga taataagtgg aaaacaataa tgatattaga
    28681 cattttagca ttggggaaag agttacaaca gtaagtgaat tatcaaagga ttatagagat
    28741 aaagagggaa cgattatttc tcttaataag aatcgaagag gcgtttggca atatgttgtt
    28801 gatttaggtg atggcgttac agttgagtgt aaagatagtc aactgatcag aaataatggc
    28861 agtcattcca ttgaaaaagc aattcttgta tacccaaaag tatcaagtaa gatcatgaat
    28921 gactggttgc ttggaaaatt tggtgagtac aataaaaaag aaatcgcaga agtgatcggc
    28981 aaatcttatc caacagtatt agggtatcta aaaaagataa cgagtaaaga agcaaaaatt
    29041 gaggatgttt taacagtaag agagcttcaa tcattaatcg agtatcggaa taaacaaata
    29101 acaaatcctg agtaccttag aaaagaagat ggtctattga aacaattgga acaagtaaat
    29161 aaaatccgga agatgcaaaa ggtagctcct ttaagccttg atacctttaa aaaaatctat
    29221 ttgaaagaga tcaaaaaaga ctaatgtgga ctgtaacttt accgttccaa cctgtatctg
    29281 caccaagacc aagtgcaaga aggaatgctg ctgatgaaac taatgacgta gcagagaaaa
    29341 ggatttcaac ctactatgat aaaaagtatc ttgattattt gaaaaaaata aagagcttta
    29401 tcgaagatca agatttatta gatgatgaat tttactcgat tgtaaatgat cctatgggcg
    29461 caattatgga agtggacttt tactaccaga taccaaaaag ctataaaaaa gtctataaca
    29521 ttatgaagac aacagcgcca gatttagata atttagtaaa aagtgctatg gatggtattt
    29581 ttaacatctc agctatacga gatagccata tcacaggctt aattgctttt aaatataatg
    29641 tggcgaacga aggaaaaaca gttgtgagga tcaaacgata ttcagaattt gagtgggata
    29701 cttctgaagg attgaacgga gatatatggg aagctacatt tgattttaaa ccagtagcaa
    29761 cgccaagacc aaagtctaaa tttagaggaa atgggattat cacttactat ccaaaagagt
    29821 acaacgacta tttggaaaat atcaagaata cgcttaaaga gaaagatttg gtaaatgatc
    29881 aactgaaaaa ggtaatgaat gctcctatgg gggttatagc tcagatagat tatttttacc
    29941 aagtgagaaa agataagaaa aagcttgatt cactgatgcg tacttcacag ccggatattg
    30001 ataatttagt caaaggaaca ttggacggaa tttttaacaa ggaaataggc atcaaagata
    30061 gcagggttgt agggttaatt gcttttaaat acaatacttt tgaaaagtcg aaaacaaacg
    30121 tgagactaca ggagatgggc tgatgaataa taatgaaaaa attcttcatg tacttgatag
    30181 ctttgaaata attcaagaag aactaaagaa atatcgggat gttttggaac aaagatatga
    30241 ttttgtcaat cagcaaaaga gtaatcacat ggatttcatt ctcaatatga atgacttgaa
    30301 gaagaaatta gttgaacgaa aagaacaaga aaagttgatc aaagcttact ttgaactcgg
    30361 tgaaaaagaa gttaaaaatg caatggaact taatgaagag cgcagggttt tagatcagct
    30421 gttagaacaa ttattggtga tgtttcaaaa aggacgaata gatgaggatt taattgaaga
    30481 aggattgaga aaatatcctt caaactcagg tatcggaata gtgctgaaag cgattgatga
    30541 agaagaaatt gaagctttca taccgccaga ggattttgaa tcggcaatgg aatatataaa
    30601 atattatagc caagggatca ctgcttttag agagtttgat cctgaagatg ttattcatga
    30661 cttgaacaac ctaaaagaat ggtgtgaaag ttatggagtc gatgattccg gattggacta
    30721 cttgatcagt attatggaaa tcgaagaaga gatgccggat aagcctgatc caacagatat
    30781 tttagaattg atccatgaag ctagaaatcc aattgcctat atttctagag gatatactgt
    30841 attggaatac tataagcctt atatatcagc gatgaaccat ttaagacgtg tattaaggga
    30901 aaaacgtgaa taccgctcag tgttgaatgc aacgaataga ttagaaaaag ctgtttcaga
    30961 gctagatgct tattatcgtg aacattacct gcaagcaggt ggtatgccaa gaaatacgaa
    31021 agcaaatatc agtaggtata taaaaaaagc tgagtaggcg gagttagccc ataaaatggc
    31081 aaaaggtaac ctgaaagggg cattttgaat gaaaagggat gtttttgaat tgtacgtatt
    31141 agaacgtcta gaagacggca tgatggcaca agagataatt gatgaaattg atgatatgat
    31201 cagcagattt ggtcaattgg agttgaatga tggaaacaaa aattcggtag tgctaaaaaa
    31261 attttggttg aacaattttt cgtataagga ttttatcaaa gaaaaggcaa atacattgta
    31321 ctgactaaat tcacgaggag cagtgatgag agcttattgc taagagcagt taggctcttt
    31381 tttgtttgtg taaaattatt tgttttataa tacacattta tacacaaaaa aatattgacg
    31441 gatagtacac aaatgtggta ggatatattt gtaagcaagt tagattaaca agttaaagga
    31501 aggaagagca gttatgaaaa aagaagaact aatagatata tttcaaattg ttgaacgagc
    31561 aaataatatg ggaattatgt tttttgatcg tatttcttta aagatggatt taagcgttgc
    31621 ccaccaagaa tttaatctgc gattaaaagc cttgttgaat tctgatgatg ttaatttcgc
    31681 tcatgatgtt gtaggtatac agaaccatat agatagagaa aacaaacgta tgggtgatga
    31741 attcttacca agatattcaa gtttatgatt tggcgaaaga ttcaagagta tgtgtcatta
    31801 ttgataggta ctcttgaatt ggaaaggagg aatgaattta agacaacaac gattatgatc
    31861 ttaaaacaat cgtttatgta attggcaatc ttacgaataa ttttcatgca ataatgacaa
    31921 taaaaaatga aaaggggtaa aatcaaatga aaaaattttt agtaggaatt ctattcacaa
    31981 tggggctagt attattatca ccttcagttt cagcagaaag ctctatttcc atgtatcgta
    32041 tgtataatcc caatagcgga gagcatctat acactagaag cgttggagaa agagacaatc
    32101 tgaaacgtta tggatggcat tacgaaggtg tcgcatggca agctccaaca agtggtactc
    32161 ctgtttatcg tttgtataat cggtataacg gtgaacattt ttacacagtt gatgccggag
    32221 aaagagataa gatttcacac acaggatgga cgtacgaagg tgttgcgttc tattcctaca
    32281 acggagcaaa tagagttcct gtaacacgtt tatataacaa atacgtgaat tggcatcact
    32341 acacgttaga tcaaaatgaa aagaacgtaa ttagtaaaca aggttggaag attgaaggta
    32401 caggctggta tgcaatgcca aacagtgtac caaatcctac acctgctcca agcactgaaa
    32461 aagtgttgaa cgttcctgtt gtatggcaag gaaatacaat gttatgcgaa ggagcttctt
    32521 tattagaagg tttacactac aaaggtgtag ccacaaatca aagcttaacc agttttgtaa
    32581 actatatgcc aaaagctaat gacaataatc cttatcatgg ttactcagga gagtggcgtc
    32641 ataacgttaa tggaacgtat caaggcatga tggctgaccc agtagttaaa tgggcaaaaa
    32701 acgttggcgg taatgccgtc aatatcactg gttcaggaac gaatggaatc aagaatgaaa
    32761 ttcgcaatgg taatccggtt gtaacttgga ttacttataa ctatgctacg ccagagttca
    32821 aacaaatgcc ttggggtcgt gcagtatgga atggacatgt agttctcgtg gatggcttca
    32881 gaaatggtgc ataccatgtt gttgaccctg tatttggcgc aaaatggatc aatgcaagca
    32941 cgtatgagag agcatttaat gtgactggaa tggcggttgc tgtacgttaa ttgatagaat
    33001 tatcaactaa atgaaataag agcttaatta ctgaaatggt aaataggctc ttattttttt
    33061 agaggaaatt tttgtaatac gaatctataa caaaaaatat tgaatgtagt acacaaaagt
    33121 ggtatattaa aatcgtagag attatgcgaa gaggtgattt tgatgaacca aagaagaaga
    33181 aaaaggttag aagaaataag cgagcgactt tccgagctaa aggatgagtt agaagtaatt
    33241 caagaagaag agtacaaaga aaatatacct aaaaatctac agggatcaga aagatatgaa
    33301 aaagcagaag aatccgttga tcaacttgaa gaagctgttg atggtttatc cgacatatta
    33361 gaattgattg aagatatagc tagctagaga tgattaatga agatttcagg agtgagaata
    33421 aaatgattga tatttcactg atagagaagt tacttttctc aaataagtac accgcaaaag
    33481 agctggctac aagatggcaa atatccgaat cagctattaa agcctatcgt caaaataagg
    33541 gaagcgtagg atggcgtgat tggaaaggga tcagcattgg aaaagcagaa gacataatga
    33601 aacgtgaagg acttcgatcc tataccactt ctgaaaaaga caaataactt aatgtttgaa
    33661 aagataaaat atctgtctta aagctatgtc aaattcaaat ggtgaatgtt tgctaaatat
    33721 aatgcaaaaa aaggaaggga aatattaaat gataagcgaa aatttgtctt atgagaataa
    33781 taaacatcta gttaaagaaa ataagaaagt agaagattat aagaacatgt atatagaaat
    33841 gaaaaaatca ccactgtgta ttcaatataa aaatgataat tggaagcaag tagtcgaatt
    33901 tgtaaaacct catagtatac gaagagtacg aagggggaag aaaaaaagtg aacttgtaaa
    33961 tagcggaaga gcggtaatta gagttataac caaagattat ccagttgaat atggagatta
    34021 tattttaagg ttaagttatc atgcttttgt tgtgttgaaa gaagagcatt tcaacgagtt
    34081 tttcaattta aaaagtaagt aatcatattt ttttgtagac gtgtataaag ttaactaaat
    34141 tgcgaggtga tgacatggta gcaaaaaaga atactctaaa agaagcttct gaaaaatatc
    34201 aagatatgaa aagacgagga ttaataccga aagacaaaaa aggtatatgg agagaatttg
    34261 ttaaggaagt cagtagtgat cgaagtggcg gtgactttgt tggacgatat acagcaaaag
    34321 gctatagcaa acgattaagg atcactgttg aatatggaac gaatgaatca gctacagagc
    34381 tgaagcaaag gtacttagat acacgaaggg aagcttcaaa aaaatataat gatttcaaga
    34441 gcagaggtct gattcctaaa aagaaaaagg gaatttggcg tgagtttttc aaggaagaag
    34501 atagcaatcg gaataaggga gacttcattg gacgatacac agcaaaaggc tacactaaga
    34561 atttagggat caaggttgaa tacggaactg gtgaaagctc atgggaattg agaaatcgct
    34621 atttaaatac attgttagag aaatataaag cagaattatt ggagttgttc gatgttaatg
    34681 attttatcga gatggctgaa aagtatggtc atgaagaagg ttttatgatc aatgacatta
    34741 tggttattga taattttacg gatttattag cacttgcgaa aaaagcaagg gatttaagag
    34801 aaacgcagat gtatctattc agaaataaaa acggaagaaa ccggagaact gaatcgagtg
    34861 agattgaaga ttactacgat gcctttacac acgatctggg agcctttgta gacgagataa
    34921 gtggtgtaat gaacaaatat agggtctaga ataaaaaagc cggagatgag cagataaatg
    34981 ggaaacaata gcatatacac acctgaaatg cagaataaaa ttaaaagact tgtcggatta
    35041 gggtggatga caagaggaaa attagattct tttagtacga tcaaatatct tttgatggac
    35101 atttaccgtc agaacaagca aacaggaata cattttttgg gttatgataa gccggaagat
    35161 gcacctttct ttgttccaag ttcagcctat agagccagta cctccggtag catgtataac
    35221 ttggatttgt ttgaggaagg agcaaagatg atcgaatatg atttcaacga agcctattcg
    35281 aggatcatga gaaattatcc tttgccgtca aacacttatt tgaaagcaaa atataataca
    35341 gatcaagcta ttttgagagc gataaataga ccaaaagtaa gaccgtatga aaatttaaaa
    35401 acgtatatgt tctttttggt taattttgaa ggaacagcca aagaaggaac atatgctaat
    35461 ccaaaatcac agtcgatgtt tgcggattat ggcgaaaatc ccaaaacagc taatgagcct
    35521 atttggttat cggagattga gctaacactt atccttgatt attatgatat taaagagatg
    35581 gaagtcctag atagccacac atttttgacg aaaaaaggat tactcaaaga atattttgaa
    35641 agaatcgagc cattaaaaac tgatcctgat acgttgaagc tttataaaaa tcttagaacg
    35701 aaagtatttg gtgctatcgg acaaaaatca ttaagagaag gagaaacaag caccttcgat
    35761 aaatacagta tgtataatcg agcgtattcc tccatggtag caggcgtatt tagagatcgt
    35821 atggttagat atgaacagaa atacgttcat agcgcatatg ggctattaaa aattcggact
    35881 gatggattgt atttcagatc cgaagtacca gagtttgaag agctgtataa acttggagta
    35941 gttaagaaaa aagttcatac aatatccaaa gaagaagtcg aaaaagcttt agaatttaaa
    36001 aacaaaggat ttgaaaataa gggctccttt gggaactcta aagatgaagt taagggtgtt
    36061 gtgatagacg atgatttcga gtttgatttt gactaaatac tctttggaaa agatggtagg
    36121 aggtaaatat gaagaagaaa attgtggaag gaaatgtagg tgttattatt ctaaaagatt
    36181 caactgacta tgctaaatca atggcactaa aagacgaaaa agttgcacgc atggataaag
    36241 atttagttga atttctgatg gatgatacag aaagattaaa aggggatcag tttttgagtt
    36301 taatgaaaga caaaggctta aacacatatg gtttatttga ttataagaaa ttagctatag
    36361 agtgggtaaa agaaggagaa atatttttta ttgaagaatg ggaaggtact gagaacttag
    36421 ttcttcatca gtatcttttg aaagcttaat ctaagtaaaa cagttctgac tagttatgct
    36481 tgtcagaatt tcaaaggatg ttttccataa ccatcatcat tcaaaaataa aggatttgaa
    36541 aataagggtt cctttgggaa ctctaaagat gaagttaagg gtgttgtgat agacgatgat
    36601 ttcaagtttg attttgacta gatttttttg aaaggtttaa gagtaaacaa ggtaagaact
    36661 aagatcatga agattgagag taaagaagat agaggacgaa tgtatgagaa cacactatat
    36721 ttatttagaa gataaaattg tagatgaaat tccggcaaaa agtcgagaac aagctagaaa
    36781 attatttatt gaaaaataca aagagatcaa tggagatagt gaagttccaa aagtggtcgt
    36841 aacagttaga aaaaggcaaa caaaaaagag aagtacgttt catgtcagcc ctgatatata
    36901 taatcttata gagcgacata gaagtgaaga taatatttct atctctcaat atattgctac
    36961 agttattgat gaattgattt caggaaagta tgactatgct tttgattttg aggatgaaaa
    37021 ggattacaaa agtgttgctg tctttcaatc tgattttgag aagctaaaaa actatgctgg
    37081 caggagaaat gttaattttg caagactact agaagagttc tactccgaag agttgggagt
    37141 aggaaatgaa tacaagagag aaacttatgc gaaattgtct cataagttat cccaagcgct
    37201 taaagaagtg acagaagtta aagcggaact agatgctaaa cttgaagaag tgaaaaaata
    37261 tcaaacagag aggaagaaca acatgaagaa tcaagtggaa tttgacgaac agaaagaatt
    37321 tgaaaaagca agaaaacgct ataacaaaat tatgaatgaa cttagctatg aacacttaac
    37381 agttcatgaa gaattgagtg agggtacaga aaaatatacg atcgcagata tggttaatga
    37441 agtggaaact cttttagctt ctttctttga aaatggtcac gtaaacgatg aatggcgaaa
    37501 agaagaacct aaaagggtta acgcttatat tgctcgatta aaaaggttta ttgataaata
    37561 taaggagaat agtgacaaat tttctaaaga taaataagga gagagtgtga ttacatttgc
    37621 cagaaatcga aggaatattt tcgccaaaaa taggtaaaaa acagttagat ttcagcagtt
    37681 ttccgagcgt aaatagagat tataatctag gtgatgaggt acagatacta aatattgatt
    37741 tcaaagaact agacttcgct gttgctgttt ccaacaaacc caaggcattg gaacgagtga
    37801 aaaaaatata ccaagagaat gatcaaaata tcaaaaatat gatcaaaaag tatagacgag
    37861 ataattcaaa agttgaagac gttttattca acggtacggc aaaacatgat ttgaatgttt
    37921 atatggcatt aggagtggct ttgtactgta attcaattaa tcagacagat attttggtta
    37981 atatcgtgag gaatagctat caaaaaatat taaaaataag tagcgaaaca aacccttatt
    38041 atgcaatgaa atatcttggg gataatttat taaatgataa aaaattgatg cacaacactg
    38101 aagagctgtt atctaatttg gctgcgatta actttctatt caaaacaggt tctgaagatt
    38161 attttataga taacgtacta aaaggtgctt acggaacagc attttatccg ctttacaata
    38221 tgaaacagca taaagccgaa caaaaggata acaaacttga aattaaaaag atgaaagaac
    38281 tattaggtat aggatttgta aagcccaatt caaaagtcac tattgcttcg ttagcaacta
    38341 ctaacaatga tgggaaaata gtaaatgggt ttgacgattc agatatagat atattgtcaa
    38401 aatcatattt ttctttgtat ggagattttg cagacttgat gggtattgat agttacttag
    38461 tcaataattt tgagatcaag ggtagcgagc tgaatgatat tgtgtatttc gttttaatct
    38521 ctctatatgc aattgatcag aataccgagt accgacaaat tgactatttg ctagtttttt
    38581 atagtttgtt atatcagttc gttttaactc ggttgtttaa aaatgctaag gaagaactga
    38641 tcctgaattc tgattatgaa atagcaaaac aaattgcaag tattagaaaa gaaaacaaat
    38701 ctgaagtaat caaattaaga aaagaattaa gtattgcgag taaagaaaac ggtcggttag
    38761 aatctttagt caaatcggca aaaaaagaag aggaactttt gaagcaagag attaaagaat
    38821 taaaaggaat cagaaaagaa tttgaacagc tgaggtttga aaataaagta ttaaagcaat
    38881 tgcaggaaca agaacataga caatcattaa atggtaaaac tgaagagcct tcactgtctg
    38941 aaatgatagc ttatctaaaa cataaaaaca ttcttttagt gggaggacat gtaaattttc
    39001 aaaataaatt aactgaacta ttaccaaata ttaaaatgtt tgatgtaagc gaaaataatg
    39061 gaaagatact taaaaatttg aatacgtgtg atgcgatttt tctatataca gattacttga
    39121 atcatggtct atattatcgt gttatgagtg aaatttctaa taaagataag atattttatt
    39181 tggataatca tgtgaatatg aacttaacaa ttaaacgtat ttacgatctt ttatcactta
    39241 tataaagaga gaagtgataa ataggacaca aaatttggaa agtaggtaag tcactatgag
    39301 agaactaact ttatcttctg aggaattaga aaagaaaact gctttattaa acgaaatatt
    39361 tagtgctgat tgtataatcc aaagtttaaa agaaagttca ttttcaaaac taacagatca
    39421 gaaaaaaatt tcttatatgc taagtttaga taatctagga actaaatatg atgaatcggc
    39481 aagtagcgat aagcttttga ttgaattagt atcaagtctt agtagttttg gaaaaatgaa
    39541 aagatttaat gtgatattag aaatttttgc ttggataatc ccttcattta tttggttttt
    39601 ttatattttc atagttgaat ctgagttaat agccaaggtc tctcttgaac tgttaattcc
    39661 gatatatgca ttttatttaa taggttcagc aatttacttt aataacttgt atacacgtat
    39721 tcaaataaaa aagcagttat tacatgaagg attaagtggt aatatgttaa aaagaattgc
    39781 atgggataaa gataataaaa cagttattga gcttaaagaa tataaataca attatttctc
    39841 gagatgtata gatagaaagt tactgtttaa aaaagaagga ttagaattta gttttgatga
    39901 agatgaattt gaaaaattag tgatggactg gcgtaagaat ttactggata cggtacaatt
    39961 ctaaaagcaa ttgaggtcga aattgcgata cttgaatcat ggtctatatt atcgtgttat
    40021 gagtgaaatt tctaataaag ataagatatt tcatttggac agtcatgtga atatgagctt
    40081 aacaattaaa cgtatttatg atcttttata tgtttataaa cattaatgag gtgctaaaaa
    40141 tgacaacaat aactatgaca aaaaatatag aagtatatgt tgatggatca caagaagaaa
    40201 gggattcctt ttttgatttt ttttggaaaa atagtagaga atatgtaaaa gcgttgaatc
    40261 tggtatataa acagctaatg tttgaagagt tgataaaaga tgcaattaaa gaaatggatc
    40321 aaggttatgc tgagagagaa gaaagtattc aaaatcggat tgaaaagtta gcatatactg
    40381 atccaataga tcaaaaaaaa cttgataaag caaaagctga attatctaaa ttgagaaatg
    40441 aaaaaaataa agaagcgaga gaaatattaa cgcaagcgat agggttgaag agtcaaacga
    40501 gaacacgaga tgtagcaaaa aaattagact ttgactttgg agattttgtt gattcagcaa
    40561 atatgaaagc tagtaaggat tttggcaatg attttattgg aattgttact ggtcaaagaa
    40621 cacctagaat ctatagaaat aaaagtaaat acggattgtt accgttcaga ggtcgggata
    40681 ttaagattca caaagctgac aagttttata tctctttacc taaaggattt agacttaaag
    40741 taaatttagg tagcaagcca agaaaagcaa gagatgttgc ttcagtttta caacacatta
    40801 tggatggcga ttacaaatta aatcaaagta atcttatttt tgatggaaga aaattatttt
    40861 ttaatctaac aatttctttt gaaaaaagct ataatgaaat ggaattcaag aaaggtaatg
    40921 aattgatcgt aaactttgca gatttgtgta gcggtgaatg tttaattaat gataaatctg
    40981 taatgtcttt tggtgatagc caatatataa gaaacttcaa aagtaaaatt ttagcaatga
    41041 aatcaaaaga acaaagccga ggagtctttt ctaagggcgg tcatggtcga aaaagaaaat
    41101 tagatcaaga tcgatgggaa gcaataaaga aaagagaaag taactttgtt aaaacttata
    41161 ataatcaatg ttctgcaaaa atcgttaaaa ctgctattcg caataaagct caaattattc
    41221 gtatcattga accagataag gcttatggcg aatgggctta ctatcaattg aaagaacaac
    41281 tagtaaacaa aggaaaaaga tatgggatag aagtcagaag ttagtaattt aaacaatcgt
    41341 tgatttggca acgaaatagt gagggaaaat ccactcactg aagggtaaac tccgtcaagt
    41401 tgtgtgacca gacggaagga cgaatgttgt tgaggtttta gaactatata aaacagcatg
    41461 tttctcaaat tatgagatgt taagaaaaat gattcgagag gttttagaac tatataaaac
    41521 agcatgtttc tcaaacaaag tgcagaacga aaatcaattc atcacagttt tagaactata
    41581 taaaacagca tggttttaaa acaagaaata agtcagtttt ccttttgctt tagtaaggaa
    41641 aaatagagta ttctcaataa aacttaaaat aagaaagaat aagcaaacct ttgttttaat
    41701 agctaatcga agtaaaaccc aaagccatat gaactcctga atcagcgaaa gtttatgatg
    41761 attgggctta ctaataattt aggaattaga tggtaaatct aaattaaaca atcgttgatt
    41821 tggcaacgaa atagtgaggg aaaatccact cactgaaggg taaactccgt caagttgtgt
    41881 gaccagacgg aaggacgaat gttgttgagg tttagaacca cataaaacag catgtttctc
    41941 aaattgcctt agtggttgaa ttgagatcta tcacgtttta gaaccatata aaacaacata
    42001 gttctcaaat tgtttaaaat gattgtcaag ttaaaaaaat ctgggatcat ataaaatagt
    42061 atagttctca aatatgatta gaaaccctat taaagagcaa tattaaacca acttacttaa
    42121 atcgtttaat taagaacagt aatctctttt agtcagactt ttagtctgac ttttttgtat
    42181 ttccaaaatt tattctttac aaaagcgaac gtacgtttgt ataattgctt gtgaatgggg
    42241 gaataaaaat gtttgattac agtagagaac cggtgaatga ctatttttta atcgacatga
    42301 agtctttcta tgctagcgtg gaatgtattg aacgaaattt agacccaatg actacaccgt
    42361 taattgtcat gagtagatcg gataacactg gttcaggtct gactctagct tcttcaccta
    42421 ctgcaaagaa aaaatatcat atcactaatg ttagtagagc taatgattta ccttatccat
    42481 taccaaaaga actttatatt gttccacctc gaatgaactt atatataaaa aaaaatatgg
    42541 aagttaatca gatttttaga aagtatgtcg cagatgaaga tttattgatt tattcgattg
    42601 atgaatcaat tttaaaagtg acaaaatcct taaatttatt tactagttca gaaacaagaa
    42661 gcgagaggag aagagaatta gctaaaataa tcaaagatga gattgagaaa gaaacaggac
    42721 taatagcaac agtcggaata ggagacaacc cattattagc taaactcgca ctggacaatg
    42781 aagcgaagag tagtaaagaa tttatagctg agtggactta tgagactgta cctgaaaaaa
    42841 tatggaaaat aaacgagatg actgactttt ggggcattgg atcacgcatg aaaaaaagat
    42901 tgaatcatat gggaattatg agtatagaag atttagcaaa ttggaatccc tacacaatta
    42961 aaagtcagtt aggtgtaatc ggacttcagt tatatttcca tgccaatgga attgatcgta
    43021 cggatattgc tattccacca gaaccaacca aagaaaagtc ttatgggaat tcacaagttc
    43081 taccaaaaga ttatacgagg agagaagaag tagaactagt agtcaaggag atggcagagc
    43141 aagtagccat tcgaattaga caacataatt gcaaaactgg ttgtgtacgc ttagcaattg
    43201 gtacgtcaat tttggaagaa aaatcaggtt tctcccacca gatgaaaata ccaattacag
    43261 ataatacgaa agaattacaa aattattgtc tttttttatt caataagttt tatgaggggc
    43321 aggaagtaag acatatagga gtaacttata gcagattagt atacacagat tccctacaat
    43381 tagatttatt tagtgatcca agaaaaaaga ttgctgaaga aaatttggat aggattattg
    43441 ataagattag acaaaaatat ggatttacag ctattataca tgcttcatct atgttagaag
    43501 gtgcaagaag tattacgaga agcactttaa taggaggaca cgcaggagga ttaggaggtt
    43561 tgcataatga ttgaatataa cgatagagga cgtttaaaat ggtcgggatt ttatttgtct
    43621 gatcatactg aaagaatcaa tgcagacaat gtacaacgtg ctaatcataa tttggcaaaa
    43681 gaacaaatga cgactgaaga gattgcgaaa atactgagaa ctgctgtcat aaaaaataga
    43741 cccgtctgca ttcaaaaaga ggaacgaaac gctgaaggtt actatccaga tgatgtagta
    43801 ggaaaaatta ttggatatga cgagcttggt gtatttgtgg aagaagaaaa agttcactat
    43861 gatgaaatta ggaatgtttc tttcgcggaa ttattaaaat ggagtcagat atgaacatta
    43921 ttaaaaaata cgaattcggt tatattacgt atgaacagct aatagaggaa ttatggggat
    43981 atggtcaaca attaattaat gaagtgggga ttgattgctt ttgtttctat attgaatccg
    44041 gagcaggata ccataagaac agatactata tatttccata tccaccaaaa tagtctctta
    44101 gagattttta aattttctcc aaatactttc aattattgtg aaatatttcc ctttaacaga
    44161 attaggcaga agtcgcccat cctctatagg taggtgatga atgccgttcg gtatgagggt
    44221 taccgttggt aactcgaaga ccgacaacta aggcgtattg cctttttatt ttgtcccatg
    44281 ttacaatcag tctatacaag aaatgagttg atataacatg gaattagata caaataatca
    44341 ttcagtattt cttctttatt accaccttgt tttagtaacg aaatatcgta gacaagtgat
    44401 tgatgatgaa atatctgatt atgccaaaac tacttttgaa agaatttcag aatcgtatca
    44461 cattacttta gtggagtgga atcatgataa agaccacgtt catattatgt tcaaagctca
    44521 acctaaaaca gaattgacaa aattcatcaa tgcctataaa agtgccagct caagattgat
    44581 aaaacgtgac tttccaagag tcaaacaatt tctttggaaa gaaatgttct ggtctaaaag
//
LOCUS       DACDRN010000014        44041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDRN010000014 DACDRN010000000
VERSION     DACDRN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.014"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..384)
                     /locus_tag="IWJ80_001017"
     CDS             complement(<1..384)
                     /locus_tag="IWJ80_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460766.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIF"
     gene            complement(681..3062)
                     /locus_tag="IWJ80_001018"
     CDS             complement(681..3062)
                     /locus_tag="IWJ80_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9460767.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPVFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(3052..3699)
                     /gene="recU"
                     /locus_tag="IWJ80_001019"
     CDS             complement(3052..3699)
                     /gene="recU"
                     /locus_tag="IWJ80_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP9460768.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3763..4302
                     /locus_tag="IWJ80_001020"
     CDS             3763..4302
                     /locus_tag="IWJ80_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP9460769.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4392..4811
                     /gene="gpsB"
                     /locus_tag="IWJ80_001021"
     CDS             4392..4811
                     /gene="gpsB"
                     /locus_tag="IWJ80_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP9460770.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4833..5200
                     /gene="rnpB"
                     /locus_tag="IWJ80_001022"
     ncRNA           4833..5200
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWJ80_001022"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5409..6575
                     /locus_tag="IWJ80_001023"
     CDS             5409..6575
                     /locus_tag="IWJ80_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP9460771.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6621..8117
                     /locus_tag="IWJ80_001024"
     CDS             6621..8117
                     /locus_tag="IWJ80_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP9460772.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     LLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            8198..8881
                     /locus_tag="IWJ80_001025"
     CDS             8198..8881
                     /locus_tag="IWJ80_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9460773.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9526..10716
                     /locus_tag="IWJ80_001026"
     CDS             9526..10716
                     /locus_tag="IWJ80_001026"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP9460774.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10818..11771
                     /locus_tag="IWJ80_001027"
     CDS             10818..11771
                     /locus_tag="IWJ80_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP9460775.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11840..13189
                     /locus_tag="IWJ80_001028"
     CDS             11840..13189
                     /locus_tag="IWJ80_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9460776.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13372..13695)
                     /locus_tag="IWJ80_001029"
     CDS             complement(13372..13695)
                     /locus_tag="IWJ80_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460777.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13824..14987
                     /locus_tag="IWJ80_001030"
     CDS             13824..14987
                     /locus_tag="IWJ80_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460778.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            15057..16406
                     /locus_tag="IWJ80_001031"
     CDS             15057..16406
                     /locus_tag="IWJ80_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9460779.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16451..16882)
                     /locus_tag="IWJ80_001032"
     CDS             complement(16451..16882)
                     /locus_tag="IWJ80_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9460780.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            17270..19912
                     /gene="alaS"
                     /locus_tag="IWJ80_001033"
     CDS             17270..19912
                     /gene="alaS"
                     /locus_tag="IWJ80_001033"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP9460781.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            20112..20708
                     /locus_tag="IWJ80_001034"
     CDS             20112..20708
                     /locus_tag="IWJ80_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9460782.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20830..21978
                     /locus_tag="IWJ80_001035"
     CDS             20830..21978
                     /locus_tag="IWJ80_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9460783.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(22016..22429)
                     /locus_tag="IWJ80_001036"
     CDS             complement(22016..22429)
                     /locus_tag="IWJ80_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP9460784.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22508..22939
                     /locus_tag="IWJ80_001037"
     CDS             22508..22939
                     /locus_tag="IWJ80_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP9460785.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(23038..23271)
                     /locus_tag="IWJ80_001038"
     CDS             complement(23038..23271)
                     /locus_tag="IWJ80_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP9460786.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23454..25124
                     /locus_tag="IWJ80_001039"
     CDS             23454..25124
                     /locus_tag="IWJ80_001039"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP9460787.1"
                     /translation="MKNDLQISQETELEPIVEMASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            25271..25657
                     /locus_tag="IWJ80_001040"
     CDS             25271..25657
                     /locus_tag="IWJ80_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460788.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25644..26342
                     /locus_tag="IWJ80_001041"
     CDS             25644..26342
                     /locus_tag="IWJ80_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9460789.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            26347..26811
                     /locus_tag="IWJ80_001042"
     CDS             26347..26811
                     /locus_tag="IWJ80_001042"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP9460790.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26808..27719
                     /locus_tag="IWJ80_001043"
     CDS             26808..27719
                     /locus_tag="IWJ80_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9460791.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27954..28493
                     /gene="pyrR"
                     /locus_tag="IWJ80_001044"
     CDS             27954..28493
                     /gene="pyrR"
                     /locus_tag="IWJ80_001044"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP9460792.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28507..29790
                     /locus_tag="IWJ80_001045"
     CDS             28507..29790
                     /locus_tag="IWJ80_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP9460793.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29803..30729
                     /locus_tag="IWJ80_001046"
     CDS             29803..30729
                     /locus_tag="IWJ80_001046"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP9460794.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30748..32028
                     /locus_tag="IWJ80_001047"
     CDS             30748..32028
                     /locus_tag="IWJ80_001047"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP9460795.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            32033..33109
                     /gene="carA"
                     /locus_tag="IWJ80_001048"
     CDS             32033..33109
                     /gene="carA"
                     /locus_tag="IWJ80_001048"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP9460796.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            33114..36299
                     /gene="carB"
                     /locus_tag="IWJ80_001049"
     CDS             33114..36299
                     /gene="carB"
                     /locus_tag="IWJ80_001049"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP9460797.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            36353..37129
                     /locus_tag="IWJ80_001050"
     CDS             36353..37129
                     /locus_tag="IWJ80_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP9460798.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            37126..38058
                     /locus_tag="IWJ80_001051"
     CDS             37126..38058
                     /locus_tag="IWJ80_001051"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP9460799.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            38055..38771
                     /gene="pyrF"
                     /locus_tag="IWJ80_001052"
     CDS             38055..38771
                     /gene="pyrF"
                     /locus_tag="IWJ80_001052"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP9460800.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38778..39407
                     /locus_tag="IWJ80_001053"
     CDS             38778..39407
                     /locus_tag="IWJ80_001053"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP9460801.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            39395..39709
                     /locus_tag="IWJ80_001054"
     CDS             39395..39709
                     /locus_tag="IWJ80_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460802.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            39747..40388
                     /locus_tag="IWJ80_001055"
     CDS             39747..40388
                     /locus_tag="IWJ80_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9460803.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40419..41123
                     /locus_tag="IWJ80_001056"
     CDS             40419..41123
                     /locus_tag="IWJ80_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP9460804.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            41120..42022
                     /locus_tag="IWJ80_001057"
     CDS             41120..42022
                     /locus_tag="IWJ80_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9460805.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(42180..43886)
                     /locus_tag="IWJ80_001058"
     CDS             complement(42180..43886)
                     /locus_tag="IWJ80_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP9460806.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        14154 a         7737 c         9539 g        12611 t
ORIGIN      
        1 aaaaatgata agtgataata aaaaaatacc atttataact aaacataaac taatggattt
       61 gggcggtttc aggctcatca gaaaaatata gattctggtg aacaacaacc ataaaaaagt
      121 gagaatcatt gcatgagagc ttctttccat aaattttcct ttaagagtag tgcttaaaag
      181 tacagaaaaa ataataaatg caaggatagg agcaattaaa gtaaatttat ttaataataa
      241 acaaaatttt tcagcttttt tattcttgga ataacgaggt attgttttcg ataaaaaaag
      301 atggtgcaga caaattatga taattaagca tactatcaga taactcaaaa tgaagccttc
      361 tttcaaaaaa ttattcaaaa ccatatattg tattttttat ttgataataa caatgggatt
      421 attaagaagt ggcactaaat aaaagactag tgtgtattta gccgatttaa ttataaacaa
      481 gtaaatggat aaagtatatg taatgataaa agaaaaatgg attttctaat ttttaagcta
      541 aatgttagat cacacatgta tatgctggat agtcttataa aaaatcatgt aatacagctt
      601 ggtacatgat ttttatttgt agctgttttt tacatagaca caaaaaagaa accacgcttg
      661 tagacgtggt ttctttttta ctatctgttt ccagaagctg aggtgctgct ggatggtgta
      721 tggtcatttg cagcatccgc tccgctagtt gctggctcac tggaagaagc aggaggagca
      781 gatgaagacg gtgtcgagct ttcttcggat gaagaagaag gaaccgttgt tgaagacgct
      841 ggctcgcttg tttctgctgt cgtgctttct tttgaggact cagcagttga ttcgctttga
      901 ctagtagagc tagagctttg agtcgttgtc tctgttgttg agcttccagg agtctggaca
      961 tagctggaag atggaatagt tgttgatggt gtaattgcat tcgatctagc tgtatattga
     1021 tctttgtagt acagttcacc gcccacacga attaatccgc ttggcatctc ccagtctgta
     1081 tttgtcacgt tagctgaaac atactgcata agctcgcgat aaacatcaga ggctacgtgt
     1141 gacgattctg aagtgacagg tgtcattttt ttattgtaac ctgtccacac ggaaatcgaa
     1201 taatttggcg tatagcccgc aaataggata tctggataca ccccagaaga aataccaagt
     1261 tttgcatact cgtcatctgt gtaattggat gttccggtct taccggcttg atataaccca
     1321 gcgatttgcg cattcgtacc agttccttca gtgatcgtat cttttaaaat gtctgtgatc
     1381 atataagctg tttctggaga catggctgtt tttccatctg gctcataagt ttcttctgtt
     1441 ccgtcttgga agacgatttt attaacatat tgaggtttgt aataagtccc tccattggcg
     1501 aaagccgcat aggctgctgc catttttaag gaagaagctc cgtattttgt cccatcttgt
     1561 tcttccgtat tactagagat cgcattcgat tgatggatcg tactgtattc gatcccaaga
     1621 ttttttaaga aagaagccac tttatctgaa ccgacttcat tgaataattt gactgctgga
     1681 acattacggg ataagtaaag tgcttggcgt aaagtaatgg tgcccatata ttgattatcc
     1741 cagtttccta caggagtcga agtcccttca tagttatacg gtgcgtcagt aatagttttt
     1801 ccagtagaat atttcaaata ttcaaaaact ggtccgtaat ctgtgacagg tttcatcgta
     1861 gaaccaaaat cacgggaagt attcactgct aagttgttgc ctaaagttac gtcttctgcg
     1921 atgtgacgtc cgccaatctg ggcttttact ttcccggtat tggtgtcgat aagggtggag
     1981 gcaacttgca tctcgtcgtc tgggtaagag acatactgat ccgtgttgac gatatcatat
     2041 agtttctttt gggcgtccag atccaaattc gtataaatct ctaaaccgtc tgtgtagaca
     2101 ttcttatctg ttttttcttg tacttcattg atgacttctt tgacataatt atcgacgatc
     2161 ttagtattgt catcggattg agttagttct tgcagaccat ccgttaccgg tacattaaca
     2221 gcttgatcat attctgtttg agagattttt tcgttttgaa gcatcgtata caaaacagta
     2281 tctcgtcttt tcttcgcctg atctggataa acataaggat cataggcgga aggggcttga
     2341 ggcatcccag caagcaatgc tgtttgcggt aaagaaagtt ctgataattt cttaccaaag
     2401 tacatctcag aagctgtctc catcccgtac aagccgtttg acatatacac tttatttacg
     2461 tagtaggtca agatctcttg tttcgatttt ttttgttcca atcttacagc catccacgct
     2521 tcttgggcct tgcgtttcag agtctgatcc tcggcactgg tggagaagaa ggaaagtttg
     2581 atcaattgct gtgtcaaagt acttccgcct tgcagtccgc cagaggtaaa gttagagagg
     2641 gcagaaccga tgatccgaat agggtcaacc ccgatgtgtt tataaaaacg gcgatcttct
     2701 accgacacga tcgcatcttc taatgttttc ggtaattcat tcgcagaaat tttttcacgc
     2761 ttttctgcgc ctaaatcttc gaatagttcg ccatcttgtg tatatagttt ggaagatacg
     2821 gtagcgtcta attttttatc cgtcaattcg ggcgcatctt ttgcgtaata ccagaaaagt
     2881 cctacacctg ctaaaaagag gatacagaaa aatgagagta ttcctaaaag gatcttgata
     2941 aataatcctt ttttatgtgt tccagaggac tttccagaac ctttgccaga attcttcttc
     3001 acactctttt taggagtagg ctgtttctgt cgtctagagc ttctagtttg ttcatttgcc
     3061 attcttgctt tctccttcaa tagataatgt tgataaatag aagtcaaccg cgtccaaata
     3121 aggaatccgc ggagaaatac cagttttgat ctctattcct agttcttcga tcaaagataa
     3181 aggtagtgat ttgcgtcctt tttccttttg ggcgtcccag taagcaatca aatgaacact
     3241 attcaaataa aaacagcgct tcaaagacga aaaccataac aagacaaagc agaccccatt
     3301 ttgttcgagg catgctttca tatgttcgat ttgatgggca tggaagtttt taaagggaaa
     3361 agatgttttg ttcttggttt cttttgcttc aaagtccaag taaaagcctc gatagacgcc
     3421 attatagtct gtagttgagg cttgggtaaa gtaggcttcc ttgatcacag cagcacttct
     3481 cttaggatag tctactttca cgatttggat aggggtaggc tttttatgga taaccgccaa
     3541 cccgcgggaa agataatatt tattactttc attgattgct tcttcaaagc gcatcccccg
     3601 attgccgaat tcagtgatct gatttctttt ttcttgaatt ttttcgacgg gagaggcatt
     3661 cttataataa ggttggccat ttggatagcg caataccatc tttaatcaca ctcctaggcc
     3721 acattatagc aaaaaagcgg atagaaagaa aaggaatctg agatggataa gtcaaaagta
     3781 atctatgtgt ctgggtatcg aagtttcgaa ctcggtgttt ttaaagaaaa tgatcctaaa
     3841 atccaagttg ttaaaaatgt tttaaaaaaa gaactccatc agttagcaga agaagggctg
     3901 gaatgggtac ttgtcagtgg gaatttgggc gtcgaactat gggctgtgca aatagtagcg
     3961 gaattgaaag aagaatatcc ggaactatct cttggtattt tgtatccttt ttctgatttt
     4021 ggtaataact ggaatgaagg gaaccaagaa aaaaaacaat tagcagaaca attagcagat
     4081 tatgtcgaag ctgttagcca tcaaccctac caatcaccta gtcagctaaa gaatcacact
     4141 cgttttatac ttgagcatac agtgggctgt ctgctgatct atgatgaaga attccctgga
     4201 aaaacgaagt atttttttga agatgcacag aattaccaag cagatcatcc ctacgaaatt
     4261 cggctgatca gtatggacga tcttcaaaat tttagtgaat aatcgatatt gtgtttgatt
     4321 tatcaacttt tttcctatac aatgaaaaag gtagaaatgt tattacgtaa atgaaaacta
     4381 gaggtgtaaa tatggcgaat ttggtttata gtcctaaaga cattttacaa caagaattca
     4441 aaacaaaaat gcgcggctat gatcccgttg aagttgatga attcttagac aatatcatca
     4501 aagattatga aacttacagc aaagaactcc ttgctttaca agaagaaaac gatcgattga
     4561 gtgcaaaagt agctcaatta tctaaaactc aaggagcagc tcaaacacgc gtgcaacaaa
     4621 cagaggcacc aaagagcgca gcagtgacca attttgatat cttaaaacgc ttgtcaaatc
     4681 ttgaacgtga agtatttggt aaaaagcttg atcaacaagc aagtgcagtc aaaccagcac
     4741 aacctaatcc aaataactac acaaatgcag acacaagctt agatgataat gaaaaaacac
     4801 gtcaatttta atttttgatt aatgaatttg cggttttcgg gtaatcgcgg tctgacttga
     4861 tcaggctgag gaaagtccat gctcgcacag gctgtgatgc ctgtagtgtt cgtgcttagc
     4921 gaaaccataa gctaaggtac tctttggagt aacggcagga aaacaagcta aggcttcggc
     4981 tatgcttaag tatccttgaa agtgccacag tgacgaagcg gctggggaaa cccatccggt
     5041 ggaacgcggt aaacccctcg agcgagcaac ccaaacaatg gtaggggcgc tttttgtctg
     5101 gaagaagaac aggacaaaaa ggcagtataa ctgcagatag atggttaccg ctttgttttt
     5161 tctccctgga aaagcaaagt acagaacatg gcttatagaa aaccgcaaac atcagggaat
     5221 taggtcgtga caaaagtaat gcgttataag aaacaagaaa aacatttgaa agctattttt
     5281 caatacttca gatgaatcgt tcttattaca aaacaaactt ttgtatcacc ggttattcgg
     5341 aatttgagta aaagtgtggg gtaggactga tgtcccacct ccttttttta taaataagga
     5401 gattgtgtat ggaaaaaaac aggaagttca acctcgtcgc aactgcggca agcgggctag
     5461 aagcactggt tggtaaagaa ttaagagatc ttggaatcga atgccaagtt gagaacggac
     5521 gtgcacgttt tgaaggaaca atggaaacga tcgctacagc taatttatgg ttacgtacag
     5581 cagatcgagt aaaaatcgtg gttggggaat tcgatgcgta tacgtttgat gaattgtttg
     5641 aaaacgtaaa agcattgcct tgggaagatt ttcttccact agatgcagct tttcctgtag
     5701 ctggaaaatc gatcaagtcg aaactatata gcacacctga ttgccaagcg atcacaaaaa
     5761 aagcaatcgt tgaacgtctc cgtacctatt atcaccgtcc agcttctgta ccactgacgg
     5821 aaactggcgc acattttcag ttggaagtag ctttgctgaa agaccatgtg atggtaactc
     5881 tggatacgac aggaccaagt ttattcaaac gtggttatcg cttagaaaaa ggtggagcac
     5941 cattgaaaga aaacatggca gcagcactta ttatgttgac aaattggcga aaagaccgtc
     6001 cattttatga tcctgtatgt ggttcaggta cgctttgtat tgaagcagcc ttaataggcc
     6061 ataatatcgc accaggattc aatcgtgagt ttgcttgcga atcttgggat tggttcagtc
     6121 aagaaatcat ggaaaatgtt cgatcagcag ccgaagaaaa agcagattat gatattgaat
     6181 tagatattac tggttcagat atcaatggac ggatgattga aattgccaaa gccaatgcag
     6241 aagaaatcgg attaggctct tcgattacct ttaagcaaca agcagtcaaa gacttcaaaa
     6301 cggataaaga atacggggtc atcgtggcta atccgcctta cggagaacgt ctaggagaag
     6361 aagaaactgt tcggaaatta tataaagaaa tgggcgatgt attccgtcca ttaaaaacat
     6421 ggagtaaata tatcttgact agcgacttag cgtttgagga attttacgga caaaaagcaa
     6481 ccaaaaaaag aaaattgtac aatggtgcat tgcgtacgga tctattccag tattggggaa
     6541 ctcgtccgcc tagaaaaaaa gagaaagacg aataatctta ctaattgatt cattcaattg
     6601 gatgtttgga ggaataggga atgaaagaaa aagagttttt acaagaagta aaagaaatca
     6661 atcttctaca acaagcttta ggaatcctcg attgggatac acaaacaggt atgcctgaaa
     6721 aagctagtgc ataccgaagt gaagtagaca gctatcttta cagcatttat ttttcaaaaa
     6781 aaatcggtcc aaaaatcaaa gaagccattc gctatttttc tgaacatccg gaagaattgt
     6841 cggaagttgg aactgccgtt tttgccaaag taaaagaaga gtatgatttg gaacatcgcg
     6901 taccggaaga tcttatgaga aaatatacag ctgccgtttc ttcagcacac ggacaatggc
     6961 aaaaagctag agaaacacaa caattttctg attttcaaca cgcattgaat caaaatattg
     7021 aattaacaaa acagttgata ccttactgga gaaaagatga aaagacgaat tatgatgttt
     7081 tgctgaatca atacgaaccc gggatgactg tcgaaatcct tgataaggta tttgatcagc
     7141 ttaaagaagg aattctttcg atccgccgta cgctccaaga aaaagggact gaacctgaaa
     7201 cagacttttt aaatcggaga atgacgaaaa aacaacagaa gagatttgta gtgaaagtaa
     7261 tcgaacaact tggttatgat tttagtcgtg gtcgtttaga tgacactgtc cacccattta
     7321 tgactggaat taatccgaat gatgcacgaa tcacgacacg atggaacgaa acagatttca
     7381 aaatggctgt ttttggagtg atccacgagg ctggtcatgg tatgtatgaa caagacatcg
     7441 atgataaata tgcgtataca ccggtttatg aaggtacatc aatgggtatc catgaatcac
     7501 aatcgttgtt taacgagatc attattggaa gtagtcgcag cttctggaaa aagcaatatc
     7561 ctttctttca agaatgtgca gaaggaacat tcgatgatat tccttttgac aaattttatc
     7621 gtttattgaa ccatacaaaa tcaagtttgg ttcgtatcga ggcagacagt ttgacttacc
     7681 ctctccatat catcattcgt tatgaaatcg aaaaaatgat tttcaatgaa ggggtagatg
     7741 ttgctgaact acctgaaata tggaatcaaa aatatgaaga gtatctggga atacgaccaa
     7801 caaatgatgt ggaaggcatc ctacaagacg ttcattggtc aggtgcaagc tttggttatt
     7861 ttccctctta tgcattaggg tacatgtatg cggcacaatt acttcattct ttgcagcaag
     7921 aaatagatgt ggaaaatgtt ctagcgtcag acgattattc tccaatcaaa aactggatgt
     7981 cagagcatat ccatcaatat ggtgcatcta gaaaaccaaa tcaattaatc atggatgcaa
     8041 cacatgaacc gcttaatccg caatacctga tcgactatat gaaagacctg tattttaatg
     8101 tatatcaggt aaaataaaat acaatttaca aagatagctg aaattcatct aatctataga
     8161 tgttttcagc tatttactta agaaagaagt ggaacgaatg gataaattac aagtgatcgc
     8221 tgattatagt atggaccaat tagcgcatga tcaaacagga catgggagcg atcataccaa
     8281 aagagttgtc aagttagctg aaaaaatttt ggagacggaa ccgcaggcag accgctttgt
     8341 gacgcttgca gcagcttacc tacatgatac gattgatgat aaagtcgtta aaaatgagaa
     8401 cgaggcaaag caacagcttc gtgtattcct tcgctctttg ccaatagtag aggaacagat
     8461 ctcgatgatt ttttccatta tcgaaaatat gtccttttct aaaaatctct ctgaagcggc
     8521 cgaactatct ctggaaggta agatcgttca agacgctgat cgtatcgaag cattaggagc
     8581 aatcggcatt ttgcgtactg cttatttcgg cggtggacat gggcacccga tttttgatcc
     8641 tgaactgtat ccgcaaacat tcaaggacaa agaaaattac cgaaaaggca cgacagtgat
     8701 caatcatttt tatgaaaaac tgttcttgct gtctgataaa atgaatactg attacggata
     8761 tgaagaagcc aaacgaagag aaaccttcat gcgtgaattc ttagaagaat tttttctgga
     8821 atggtctatt gacgatgaaa attttactcc agacaattgg atcaaaaata acaaaaaatg
     8881 atggaggttg tgacagaagt ggtcagctcc aagatatttc ggatcatttt ccgtaaaagt
     8941 tacttctgga acactgttta ccaagaggtc gtgagcctgc cgttcagctc cgagtcacaa
     9001 ggaacaattt caaaaaatag cttctcgtgt ttgatgaata ttgtaacttg tgattcggaa
     9061 ttggcaggag aacaccgttt attcggaaga ataggactgt gacaagactt ttgtcacagt
     9121 ccttttttcg aattcaacaa gtaaaacttt taagaaatat tcagagagta aaattaaaaa
     9181 ttgctgataa acgaagatgg aaattttatg tatttttatt ggtctatgca ataaataaat
     9241 taaatattca gaatacaatt gattttaacg aaagggcttc tatcaatggt tgtgttgtat
     9301 ttatctgccc aggtagagta aaatagatca ttatctaaaa ggtaattgtt catactatat
     9361 aagtcatttg aaagtaaaaa gatactgtaa ataaaaaatg tggttaaata attctcataa
     9421 tcagtgtaag cgttatcaaa aatatagcat acttgctttg tagatgaatg acacgatata
     9481 tgtttggtat gttctgcaag acagatgaat atggaggtgg agacaatgtc ttccaaagaa
     9541 ctaacaatgt atttagagaa agaattggat caactgaaag aaaaggggtt atacaatacg
     9601 atcgacatac tagaaagtga aaatggtgct tgtatcatcg tagacggaaa aaagatgatc
     9661 aatctagcat ccaacaatta tcttggattt gccaatcgcg aagaattgaa aaaagcatgt
     9721 attgaagcaa ccgaaactta tggtgtagga gcaggagcag tccgaacgat caatggttcg
     9781 ctgaaaatcc atcagcaatt agaagaaaaa atcgcagaat tcaaagggac agaagcagca
     9841 atcgcctttc aatctggatt caactgcaat atgggcgcta tctctgcagt aatgacaaaa
     9901 gaagatgcta tcttatcgga tgaattaaac catgcatcta ttattgacgg ttgccgctta
     9961 tccggagcaa aaatcatccg aattaaacat caagatatga aagaccttga gaaaaaagca
    10021 aaggaagcga tagaaagtaa gaaatataaa aaaatcatgt atatcacaga tggtgttttc
    10081 tccatggatg gagacattgc acgattgccg gaaattattc cgattgttga aaaatatgga
    10141 ctgatcacct acgtcgatga tgcgcacggc tcaggcgtaa ctggaaaagg tgcaggaacc
    10201 gtcaagcatt ttggattaag cgacaaaatc gatatgcaaa tgggcacgtt atcgaaagct
    10261 ataggtgttg ttgggggtta tgtagctgga tcgaaaacac taatcgactg gttgaaagct
    10321 agaagtcggc cattcctatt ttctacttcg ctaactccag gtgcagctgc agctgcattg
    10381 gcttcgatta ctttgatgca agaacatccg gaacttgtcg aaaaagtttg ggaaaatgct
    10441 aattatttta aagaagaatt gaaaaaagta ggctataaca tcggtatgtc tgaaacacct
    10501 attactccag tcattttggg agacgaaaaa gtgactcaga ctttctctaa gaagctgatc
    10561 gaacatggga tttatgctaa accaatcgtt tatccaactg tgcctcttgg gacaggcagg
    10621 atcagaaata tgccgactgc ggaacatacg agggaaatgc ttgatgaagc agtagcagtt
    10681 tatcaaaaaa taggaaaaga aatggaaatt atttaactaa atttagtttt ttcgtctatt
    10741 taaaattaga gaacatgttc acgcatatat tgttaatcat ctgaagatag tagtaaaaaa
    10801 gatcagggag gatcatcatg aaaaaagtat tggtgacagg atgtttagga caaattggtt
    10861 cagagttgat cacacggcta cgtcaagaac taggcagtga aaatgtcatt gcgacagata
    10921 tacgcatgcc tgaagtcatt gagaaaaacg atatctttga gatcctagat gtaatggatt
    10981 acgaaaaaat gcgcagcata gcagaacaat atcaagtgga cacattgatt catttggctg
    11041 ctcttttgtc agctgttgca gaagcaaaac cacaattagc ctggaatttg aacgtgactg
    11101 ggctggtcaa tgcattggaa gtcgctagag aattccagct gaaatttttt acgccaagtt
    11161 caatcggcgc gtttggtcca aatactccaa aagatcatac cccacaagat actcttcagc
    11221 gtcctacaac catgtatggg gtaacgaagg tagctggaga actactatgt gattattatt
    11281 atgtgaaata tggcgtagat actcgtggtg tccgtttccc tggattgatt tcttatgcaa
    11341 ccttgccagg aggcggtacg acagactacg cagtcgatat ttattattca gcaatcaaag
    11401 aaggaaagta cactagtttc attgatcaag gtacatcaat ggatatgatg tatatgccag
    11461 atgcgatcga tgccattata aaactcatga atgcagatcc tgatcgattg gttcatcgaa
    11521 atgccttcaa tatatcggcg atgtcttttg acccggaaga gatcaaagca tctattcaaa
    11581 aaatcatacc agattttgaa atggattatg atgtggatcc tgtacgtcag tcaatagcag
    11641 aatcttggcc aaattcacta gatgtttctt gtgcgcaaga ggaatggggc ttttcgccga
    11701 aatacgactt agatgtgatg acaaaggata tgttggaaaa actgaaagaa aaattggtag
    11761 agacggtata aaacaagaac cacatccaaa aagtttttta tacttttggg attggctatg
    11821 gtatgaaagg ggacaaggga tggaagaata tcaagccacg ccagtaaaag aagtaaaaat
    11881 agaaaatgaa ttaaaacgta cgatgggatt ttttactgct ttatcgactg ttatgggcac
    11941 agtgatcggt gcaggggtat tttttaaagc cgcaagcgta gcagaagtta caggatccgc
    12001 cagtttgcat atgttttctt ggtttctcgg tggtatgatt tctgtctgtg caggactaac
    12061 aggagctgaa ctggcagctg ctattccaga aacaggaggg atgattaaat atatcgaacg
    12121 gatttacgga aatacagcag cttttttatt gggctgggcg caagtcgtga tttattttcc
    12181 agcaaatgta gcagccttgt cgatcatttt cggtacacag ttcgtcaatc tatttggtct
    12241 ttcccagtca atgattgttc ccgtagctgt tacagctgct gtatcgatcc tgttgatcaa
    12301 ttttttaggt tctaaagctg gaggggcttt tcagtcgatt acgcttgtct gcaagcttat
    12361 tcctttgttt gtgatcgtca ttttcggttt gttccgacaa gaaggagtag acttccaact
    12421 gtttccgatc caagcaggag aaaatctttc atttttctca gccttgggag cgggcttgct
    12481 agcaacgatg ttcgcatacg acggttggat acacgtagga aatatttcgg gagaattgaa
    12541 aaagccggcg aaagatttgc caaaagcaat ttctttagga atcatcggaa tcatgatcgt
    12601 ttatttatta gtaaatgcgg tgttcttaag aaccgcatct atcgacggag tcgcaggaaa
    12661 cagcaatgcg gcaagtgacg ttgcaaaaat gatttttggc ggcttcggtg gaagactggt
    12721 cacagttggt atcttgattt ctgtttatgg aaccatcaat ggctataccc ttactggaat
    12781 gcgtctccct tatgttatgg caaaagaaaa caacctgcct tttagcaagc tctttgctaa
    12841 acttcatgat aaaactaaag ttcctgtagc cgcaggtatc ttagagctag tcatcgctat
    12901 tggcatgatg atgatcggtg gttttgatac actaacggat atgctgatct ttgtcatttg
    12961 gattttttat acgatggtct ttgttggtgt cattcttctt cgtaaaaaag aacctgattt
    13021 gtttcgacca tacaaagttc caatgtatcc gtttattcca ctagtagcaa tcattggagg
    13081 gacttttatc gtatcaagca cgcttatcac tcagaccttt ttagcttcga tggggatagt
    13141 attgacactt gctggtatcc cgatttatct ctatttaaaa cgtagatagc taaaacattc
    13201 gaattttatt agaactgcac ttctctgcca aacaaaatat tttatttaaa tgaggagtac
    13261 gtggaagaaa tgtcaaaagt actcgttaat tttgatcacg taaatgtcgt tttgtctacg
    13321 atgaaatagc aaagtaaaag acagaaatcg ggctcaaccg atttctgtct tttactttga
    13381 tttgcgtaac tgaactattc ggcgtacgcc gcgataaata tttaataaaa ccaagcaacc
    13441 agaaatgaaa atgggaagcc aaatcagata gacagggtag ccaagcactt tagacaaagc
    13501 atgtggagtc attttatcca tttcaagaag tgcttgctgc caagtctgaa gattgaagac
    13561 tagcatgaaa cccgaaataa tcaggagaat aaatcctaaa taatgaattg tagttctttt
    13621 tcttacataa gcgatcgtag cataaaggaa cagtaaaaaa ataggtacgc caaaaacaaa
    13681 gtacatattc agcatctcga cacccccttt gtgtttatct tactttactt tgaaagaaat
    13741 gagaaataat agcctcgtta cttgcttgaa acagcaaagg actgctaaga taagagagat
    13801 acaaagaatc aaggaggagt catatggcaa gttatatcga accgattcgt tttgcgctga
    13861 ttgtttttcc atttttagcc ttagcgatct catctgtttt ttttatttat gaatatcgaa
    13921 agtatggaac atttctagta acaagggcag ttattttgta ttctttcgtt ttttatttat
    13981 tgtgtgcgta ttttttggtg atcttaccac ttccggcaaa ggagacggtt gcccaaatga
    14041 cagggccaaa gatggagctg catttatttg cttcatggca acatttcaga gacaacacgg
    14101 tattggtctt gtctgaacct agtacctacc tgccggcaat gaagcagagt gtatttttgg
    14161 aaccagcttt taatgtttta ttactcatac catttggcat ttatttaaga tattatttcc
    14221 ggctttcttt tatgaaaacg atgattgcta gtttctgcct ttctctattt tttgaattga
    14281 cgcagctaag tggactttat tttatctacc caagaccata ccgcttattt gatgtgaatg
    14341 atttagtgac aaatacattg ggtggaatca ttggttttgt cgttacgccg ttatttacat
    14401 ttatgcttcc tacaagaaga cgaatggatg aactttctta tgaaaaagga aagactgtct
    14461 cattgactcg acgcttggtc gcttatttga tcgactggac atttttatct cttattaatt
    14521 tggtagcggc gattttcttt aaaattgcgt tcgattcagg ggcagttact tctcatatct
    14581 ggtggctttt attagaagta ggggtatact ttatgattgt accgtttata accaatggat
    14641 ggacacttgg caaaaaaatt gtgcgtatcc gtttggtgga agaagaccgc gaacgaattt
    14701 ctttaaaagc attgatgatc cgctacgggt atctgtactt tcttttttat ggtttaagct
    14761 ggttggggca gttgcttcag caaatcatga attcgacaac gaatacggtg ttgcagatga
    14821 gtgcagtatt catttctctc ggtgtcttgt tactacaagg tatttttatt ttaaatatct
    14881 tgctatcatt tttgagaaaa aaacggattc tgttttatga aaaagctagt cacacatata
    14941 ctgttagcac gattggccaa acaaaggatg atgattctcg aaattagctg agcaaaaacg
    15001 ctttgtctgt gttataatgc caaagtcgtg ataatttaaa gagaagaggt gcacgaatga
    15061 ataattttga tatattccgc ttcaaaccgt ttatcaatga agcgttaacc gcaaagggct
    15121 tcaaacagcc cacagaggtc caggaacgtt tgattccttt aattgcaaaa ggaaaaaata
    15181 tcattgggca atctcaaact ggtacaggaa aaacacatac gtttttgttg cctttgtttg
    15241 aaaaaatcaa tgcagaaaaa gaggaagtac aagttgttat tacagctcca agtagggaac
    15301 tagctgcaca gatctatgaa gcagctacac aaatcgctaa attttctaca ccggagatcc
    15361 gtgtctcgaa ttttgtcggc ggtacagaca aacagcgtca attaaatcgt ctgcatcacc
    15421 aacagccaca ggttgtagta ggcacgcctg gtagaatatt agacatgatg aatgaacaag
    15481 cgctgaagat tcacaccgca tttgcatttg ttgtcgatga agcggatatg acattggata
    15541 tgggtttttt aaatgaagta gaccaaattg caagtcgatt accagaaaag ctgcagtttt
    15601 tagttttttc tgccactatt ccagaaaaac ttcgtccatt cttacgaaaa tacatggaaa
    15661 atccaatcat tgaagaaatc aaaccaaaag cagttatttc tgaagacatt gaaaattggc
    15721 tgatttcaac aaaaggtaaa gatgagaatc aaatgattta tcaactgctg acaattggtc
    15781 atccttattt agcaatcgta tttgccaata caaaacagca tgtggatgaa attgccgatt
    15841 acttgaaaaa ccaaggcttg aaagtagcaa agattcatgg agacatcacc ccaagagaga
    15901 gaaaacgagt gatgcgacaa gtacagaatc tggagtatca atatgttgta gcgactgatt
    15961 tagctgctag agggatcgat atcgaaggtg tctctcatgt tattaatgca gaaattccag
    16021 aagatctaga cttctttatc catcgtgtcg gacgtactgg acgtaatggc ttaaaaggaa
    16081 cggccattac gttatattcg ccaaatgatg agcaagccat tgatgcgata gaaaaattgg
    16141 gcgtctcatt tgaaccaaaa gaaatcaaaa acggggaaat cgtcaaaaca tacgatcgta
    16201 atagacgtgc caaacgagaa aaatcaaaag atgtattaga tcccacgctg attggtcttg
    16261 tgaagaagaa aaagaaaaag atcaagcctg gttataagaa aaaaatcgat tgggcaattg
    16321 cagaaaaaaa taaaaaagaa cgcaaaatcg aacgcagaca gcaaagccgt actgctcgta
    16381 aaaataagaa aaattcacat aaataaaaaa caagaggttg tgacaaaagg cgtgtcacaa
    16441 tctcttgttt ttaagctaga ctttttttca ttccgataca aagtacttca tcttcaataa
    16501 aaggttctgt gcaagtatga tacccttgtt tttcataaaa atctacagcc gtcatttcag
    16561 ctactacata actagtctga caaccttctg aaaccgcccg tttttcagca gcttcaagca
    16621 actgaccgcc taaccctttt cctcggtatt cttttgctac gcaaaatcgt tccggcttga
    16681 agcaagtagg tgttttcttt tcataacgca aagtagctac tggtcttttt tgatcaagaa
    16741 atagataata ggtttgttgc gggatatctt ttccgtcgaa ttctagttct gcaggaatcc
    16801 cttgttctaa tacaaaaact tgatatctca aaaagcagct agctgcgcgt atccattcat
    16861 ccgctccaac tatttttttc atcatattcc tccttgtcat gaatagcaga gatgtattga
    16921 ttcctttatt cttatcgtac caaaaatcgt ggagtatgac caaaaggaaa tgaaaataaa
    16981 aattgagata ttgacagtga tcaatagaat gcgtatacta attaagtacg aaaaggatat
    17041 gatttgtttt ttaagactca cagaaaattc tttcttggct gaaaaaagaa tagtcactaa
    17101 tcaaattgct ccctttttct attttggtag aaataccgac gcagctcagc gttatcgagt
    17161 ttaagaggta atgattacac atcattgcaa tcaaggtggt accgcgagtt ttcgtccttg
    17221 gctgtgatgg tgtttttttg tatttaattt atagaaagca ggtaatgaca tgaaacaatt
    17281 aagtagtgca caagttcgac aaatgtttct tgatttcttc caatctaaag gacatacgat
    17341 tgaacctagt gcgtctttga tccccgtgaa cgatccaacc ttattgtgga tcaactctgg
    17401 tgttgcaaca ttgaaaaaat attttgatgg ttcagttgtg ccagaaaatc caagaatcac
    17461 aaatgcccaa aaatctattc gaacaaatga tatcgaaaat gtcggtaaaa cagcacggca
    17521 ccatacgatg tttgagatgc tgggtaattt ttcgattggt gactatttca aaaatgaagc
    17581 catccactgg gcatgggaat ttttgactga tccaaaatgg atggcattcg atccagaaaa
    17641 actttatgta acagtttatc caaaagatac agaagcaaaa cgcatctggc atgaagaagt
    17701 tggcctgcca ctagatcaca ttattgatgt agaagacaac ttctgggata tcggtgcagg
    17761 cccatgtggt ccggatacgg aaattttcta tgaccgtgga gaagcttata acgatttagc
    17821 agaagacgat cctgaaaact atccaggagg agaaaatgaa cgttatttag aaatctggaa
    17881 cttagtattt tcagaattca accataagcc tgataacaca tttgaaccac ttcctcataa
    17941 aaacattgat acggggatgg gattggaacg catggtctca atcgttcaag atgcgtcaac
    18001 gaactttgag acagacttgt ttatgcctat tatccacgcc gtagaaaagc taagcaaaca
    18061 agtaaaatat ggtgaaaacc cagtaacaga tatttcttac aaagtaatcg cagatcatat
    18121 tcgcgccctt tcatttgcta ttggcgacgg tgcattgcca tcgaatgaag ggagagggta
    18181 tgtcttacgc cgtttgcttc gtcgtgctgt catgcatggg aaaaaattag gtattgaaca
    18241 agcattctta tatcgtttag tccctgttgt tggggaaatc atggtaagtt attatcctga
    18301 agtacttcaa caaaaagaat ttattgaaaa agttatccgt acagaagaag aacgtttcaa
    18361 tgaaacaatc aatgaaggtt tggaaatctt aaatcaagtg atccaagaag taaaaaacga
    18421 aaacgggacg actctagcag gaaaagacat cttcaaacta tacgacacat atggcttccc
    18481 ggtagaatta acggaagagg tggcagaaga tgaaggactg aaagttgatc atgctggttt
    18541 tgaaaaagaa atggatgcac aacgcgaacg tgctcgctct gcccgcagta ctgaaaaatc
    18601 aatgggggta caatctgctt tattgacaga tatcaaagta gaaagtaaat tcattggata
    18661 cacagaacaa gaatcagata gtcaattatt ggtcatcgtt aaaggtgagt ctcttttgaa
    18721 tgaagtcgca gaaggtgaag ctcaaatgat ctttgatcaa acacctttct atgcagaaat
    18781 gggtgggcaa gtagcggatc aaggatggat catgaatgaa gtaggcgaaa tcgtagcagt
    18841 tgttacagat gttcaaaaag cgcctaatgg tcaatttatg cacaccgtag aagtaaaagc
    18901 accaatgagt gaaggacaaa cctatcatct attgatcgat caatcgatgc gcaatcggat
    18961 catcaaaaac catacagcaa cccatctact gcacaaggca ttgaaagaag tacttggcga
    19021 gcatgcaaat caagctggtt ctttagtcgc tcctggacat ttacgattcg actttaccca
    19081 ctttggtcaa atcacttcag atgaattagc tgaaatggaa agaatcgtca acgaaaaaat
    19141 ctgggaagct atcccagttg aaacaattga aacagatatc gatacagcca aaaacatggg
    19201 tgcaatggct ttgtttggtg aaaaatacgg acatgacgtt cgagtagtaa atattggcgg
    19261 ctggtcaatc gaactgtgcg gaggcacaca tgtagcaaat acagaaaaca tcggaatctt
    19321 caaaatcgtt tctgaatcag gaattggtgc aggcgtgcga cgaatcgagg ctgtaacaag
    19381 taaagaagcg tacgaactac ttgaagaaga agaaaaacaa ctaaaacaag tagcaacaat
    19441 tgttaaatca cctcaattga aagaagttgt ttcaaaagcg gaacaattgc aacagcaagt
    19501 aagagagcta caaaaggaaa atgaacaatt agctagcaag ttagcgaatc aacaagctgg
    19561 tgatgtattc aaagatccag aaacgatcaa tggaaaaaca atcattgcag cacaagtaaa
    19621 agtgaaagat atgaatcagc tgcgtcaatt agctgaccaa tggaaacaaa aaaatatttc
    19681 agatattctt gttttagcaa acgaacaaga aggaaaagtc agtctgctag cggcaatgac
    19741 gaaagaatca aatgaagctg gactaaaagc tggcgacttg attaaagcaa tcgcaccgaa
    19801 agtaggcggt ggcggtggtg gacgtccaga catggcacaa gctggcggga aaaatcctgc
    19861 tggtattcca gatgcactta aagctgcgaa agaatgggtt gcgcaacaat agggcgttta
    19921 cgtttgacag aaaaacaaaa gttcaaacat tgaacgaaac atgattatgg gggcgaagac
    19981 aaacaagtct tgccctcata cttttttcaa ttggatggcg catctaatca aatggtcgga
    20041 ttcggatggt ctttagttga cgaaagtgac agaaattttt acgattagta taaataaaaa
    20101 ggggtgaaaa aatgaatagt agaaataaga cctttcgact agtcttacgt gcgatcttgt
    20161 tagcaatcat catcgtacaa gcgatggttc cttggctggg gtttattcca ttaggattca
    20221 ttagtttaac aattatccat atcacagtaa ttattgctgc agtagtatta ggcccaaaag
    20281 atggcatggt gattggcttg ttttggggaa tcgctacaat tgttcgtgcc tatgccatgc
    20341 caacaacgcc atttgataca ttagtgttca cgaatccgat tatctcggta gttcccagag
    20401 tacttgttgg cttagtagca ggcttggttt tccactggat ttatcaaaaa aataaatcaa
    20461 taacgatcag ctcagccttt gcaggggtac tgggttcatt ggtcaataca gtcttagtgt
    20521 taggattcat gggattattt tatacaggtg caacggcaga tgcctatggt gtagactctg
    20581 ctcttttatt taaaacctta gcaggggttg cagcaatcaa tgggatacca gaagcaatag
    20641 gtgcgggaat cataacacca ttactagcaa aagcgttatt tgcagcaaca ccgttgaaac
    20701 cagaataatg accatctttt aatagtaata ggacgcacga tagatagaaa aaggaagaga
    20761 ggagaggaaa acaatcgtcc tctccatctt tttacctgag caacaagcaa actagaaggg
    20821 gtaaaaaact tggctaagaa acaagaagaa aaaaagaaac aaataccgca acagcggaca
    20881 aaaaaaggag tactagcggg tatcttagga ttggttacga atctgatctt gtttgtctct
    20941 aagctaatga tcgggctttt ttcaggaagt gtctccatta tggctgatgc agtgaacagt
    21001 ctatcagata ctgcttcatc catattgact ttgatcggat ttaaaattgc tgcaaagcca
    21061 gcagatcagg aacatcctta tggacacgaa cgcttcgaat atatcagtgg gttatttgtt
    21121 tcgatcatca ttacttatgt aggatttcaa tttttagagt cttctgctga aaaaatcatc
    21181 catccagaaa atgtctcttt aacacccatc gtcttttttg tcctaatcat ttccatcttt
    21241 ttgaaatttg cacaaggcaa aatgtatcag gttattgcgc aatccattga ttctgaaaca
    21301 ttgagagcta cagcaacaga tagttataat gatgtcttta ctacactggc tgtattgctt
    21361 tcagcagcaa tcgaatgggt aactggatgg cgtattgatg gatatatagg ttttgcttta
    21421 gcattatata tcctttatag tggattgacg atgcttcgaa attttgtata cgaactatta
    21481 ggaagtcgcc caacgccagc agagattgaa gaaatggaag agcatttaaa tgcttgcgat
    21541 gatatacttg gctatcatga tttattggtg cataactatg gacccaataa acgctttgca
    21601 tcagtccaca tcgaagtaga tgaatcaatg aatttgaatg tggctcataa gattattgat
    21661 ctgatagaaa aagactttaa aaagtctttg gatgtggaac ttgtctgtca cttggatcca
    21721 atgcctgttg ataacgaaaa atactataat atcttgcggc agttaaagca aattcttctt
    21781 gaaacaggta cagggcttaa tattcatgat tttagagtaa ttcatcaagg aaaaacgctg
    21841 caatttgatg tagtggtccc tgagaaattc cagtttgttg atacagaact agaaagaatc
    21901 ttacgtagtc aaatagaaga aaaaatcgga gattaccaat tggaaattac gtttgatcac
    21961 aattatcttt tatattaaat gagaaactat aaaaagactc cttaaagttt ttaatttaaa
    22021 gagtcttttt ttcttttaat tgtttatgga gagcctgacg tgcaagatta tctgctcctt
    22081 tatttttgtt ttcgggcaac cattgcagga tcaataaaga aaattcggat agaagttctt
    22141 ggattctttg aaggtaggga aggaagagct cgttcttcgt tgcgtttttg tcgaccgtag
    22201 aaactagtgt tttgctgtct gaaaaacaga aaatcgtttc attctgtaag tcgtttttct
    22261 ttagaaattc taaagttttt aaaaagactt cgaactctgc ttgatggttt gtccctatag
    22321 aaagcgggat agatagctgt tcttgtttac cttgatagac aatcaagatt ccacctccac
    22381 taggaccagg gttgcctttt gtagaggcat caatgtatac ttttaacata aaaagagatt
    22441 ctcctttttg ttcaaactaa tgttatggta agattatatc attactgaat cggtgagagg
    22501 ttagagaatg aaaaaaaata ttcgatggca gcctgaactg gcacatacga tcatctattg
    22561 gtcattgacc ttgatcattc tgttttttag tctgattctt tctttagaaa acacaggtcc
    22621 ttattggaaa agcaattttg ttttaggtat ttttcttgtt tttctatttc taggtttgcg
    22681 gcgttctttt attttaaaat caaactctgt gattatacgt tatgctgctt tttggaaaga
    22741 ccgagaaata tctgtcggac agattcgatc tttgacgcca actaatactg gggtgcaaat
    22801 gatgttgcaa gatgagaaag ccccatctgt tttcatgatg cgaaaaaaag atcaagtagt
    22861 cctttttgat cattttgaaa aaaacggacc caaatcagaa tcaattcctt atccttatac
    22921 gaaaaaaaga aaaagctaat tttaaaaatc agaacctaaa aaaacgaatt tttacaggaa
    22981 aagaaaagag tgagacgtac ggttcatccc gtttagcgtc tcactctatt tgtttatcta
    23041 ttcatgcata ctagtatcgg tagtctgaac gttgcctttc ttgacatggg cagcttgtgt
    23101 tcctcgattc ccttcgatga tctcaaattc gaccacttga ttttcttcca aagttttgaa
    23161 tccctcttct tcaatcgcag taaaatgtac aaaaatttct tctgtgtcat cataactgat
    23221 aaagccgtat ccttttttgt tgtcaaacca ttttacaatt cctgtggtca aagcactcac
    23281 gacctttcta aagtctgatc atagccttca ttataaagag tatttataag tacgtcaaat
    23341 aaattaaaaa cctgtaattt tatgtttttt ttctgaataa aaaaaggtat aattggcata
    23401 tcttaacaaa gaagatattt tgttgaataa agttcgtgaa aaggggtaat caaatgaaga
    23461 atgatctgca aatttcacaa gaaacagagc tagaaccgat tgttgaaatg gctagtaaat
    23521 tagatcttaa ggaagacgat ttggaactgt atgggaaata taaggcgaaa atcaattttt
    23581 ctggaatcaa tcgatcaaag gataacgctg aggggcattt gatcttagtt acttccatca
    23641 atccaacacc agctggcgaa ggaaagtcaa cgatcactgt agggttagga gacgcgctca
    23701 ataaaatcaa taaaaaatct gttattgcat tgcgagaacc ctcacttggt cctgtgatgg
    23761 gaatcaaagg tggtgctgca ggcggaggat atgcacaggt attgcctatg gaagatatca
    23821 acttgcattt tactggtgac atgcatgcta tcacaacagc taataatgca ttatctgctc
    23881 ttttagataa tcatattcat caaggaaatg aattgaacat cgatgcccgc cgagtgattt
    23941 ggaaacgggt agtcgatctc aatgatcgtg aactgcgtaa agtaattgtt ggcttgggtg
    24001 ggccaatcca aggtgttcca agagaagatg ggtttgatat cacagttgcc agcgaaatca
    24061 tggctatctt gtgtcttgcg gcagaccttg aagatttaaa ggctcgtctt gcaaggatcg
    24121 ttgtcggcta tacatttgac cgtcagcctg tgactgctgg agacttgaaa gcagaaggcg
    24181 ctctggcatt actgctgaaa gatgcaatca agccgaatct tgtacaaaca atttacggaa
    24241 caccagcttt tgtccacggg ggaccgtttg ccaatatcgc tcatggctgc aatagtattt
    24301 tagctaccaa gacagcattg agattagcag attacgttgt cacagaggca ggatttggtg
    24361 cagatcttgg cggagagaaa tttttagata tcaaagtacc taacttgaaa aaagcgccgg
    24421 atgctgtggt gattgtcgcc actgtccgtg cattgaaaat gcatggcgga atgaaaaaag
    24481 atgaacttaa aaatgagaat cttgatgctt tgaaagtagg ttttgcaaat ttaaagcgcc
    24541 atatacgtaa tatggaacaa tatcagctac ctgtgatcgt cgcaatcaat gaattcgtaa
    24601 cagatacaga caatgaactc acactgttgg agcacttgtg tgaaaatcag gggattcttg
    24661 ccaagagagc gagtgtatgg gcagatggag cagaaggcgg cgttgattta gcaaaagctg
    24721 tagtacgatt gattgataga aaagaagcta attacaagcc attataccgc ctagaagaaa
    24781 cgatacaaga aaagacagaa acaatcgtca agaaaattta cggaggaaat ggtgttgttt
    24841 tcagcaaaaa agcgcaaaaa caaatagaag agtttacgaa gaatggatgg gacaaactgc
    24901 cgatttgtat ggcaaaaaca cagtattcat tttctgatga tccttcactg ctaggtgcac
    24961 cggaagattt caccatcacg atccgcgaaa tcatcccaaa attaggggca ggatttttag
    25021 tagccttgac tggagatgtt atgacgatgc ctggtttgcc taaaaaacct gctgcattga
    25081 atatggacgt gacaaatgac ggagaagtgt tgggattgtt ttaagcttga ttataatcta
    25141 ccatcgaagg agctgtattt tgactacagc ttcttttttt agacgatagg gaattaattt
    25201 tgtttacact aggatcattc tttttcttta ttgtattttg agctatacta aagcataata
    25261 ggagggtatg atgaaaacga catttataag aaaaaccggt ttttacggta tgggtagctc
    25321 gattcagcta agaatcaaca atgaaaaacg cagtgttctc agtcatgaac aaaagataac
    25381 gcttgatctg gaagtgccct ttacgatgca ggtcaccttt tattggctaa aaagccccgt
    25441 ttatacagtg aaagaagcaa agcctgctta tgtgatcaca atgaattact tgattcttca
    25501 aatctatccg tttttatttc tagcaactgg gttgggggcc gttttcctac aaaatttatt
    25561 tttcagtttt tttgcagtta ttgtgttgat tgcgttcttt ttctatataa aaaaccgggc
    25621 atatttgatc aaggaggaaa attatggcaa actctgatct ttttttaatc agtttcaatc
    25681 gaatcgaaaa atggatgaga gacgagatgg gaaatgcacg aaatatggga tttaccgaac
    25741 ttgtcagacg cctagctcaa agaaaacaat taatgatcag aaaatacgaa gatgatcttt
    25801 tacagcttgc tcagttaagg aatgcgatcg tccatgatcg gatcgcagtc gattttgtca
    25861 ttgcagagcc aaatgattgg acagtcaagc gaatccagct gatcgaacaa gaactgattc
    25921 agcctgaaac cgtgctgcct cgatttgcaa aacgtgtaac aggttttgaa caagatcttc
    25981 cgttacttga actgttaaag atagtagcag aaaaaaggta ttcacaattt ccgttataca
    26041 ataaaggaaa atttgtagca ttgattactt tacgtaatat cgggttttgg ctagcaaaag
    26101 aaagccaaaa aggtccggtt gatttaacaa ataaacgagc gttggatttg atcatccaaa
    26161 acggaaagta cacaaattat cattttgttc cagctaaaac acatatttac gaggttgaag
    26221 cgatgtttcg tgagcaaggg actttggaag ccgtacttat cactaaagat gggaatcccg
    26281 atggtaatct gctcgggatc gtacgacctc gtgatattta taaacaaata gaaaaggatt
    26341 gattttttgt taattattta ttggatattc agcgctttgc tagtaggagt ggatcagttg
    26401 gtcaaatggt ggatcactga taatctagcg cttggagaga cgaaaaatct tataccaggt
    26461 attttgtcac tgaatcatat tcgaaacact ggagccgcat ggagtatgct agaagggaag
    26521 atgtggttct ttactatagt gacactcatt gcagtcgttg tgattctcac attgatgatt
    26581 aaaaatcgtg aaaaaggtaa tcgctggttc atgattggat tgagtttgat tctagctggt
    26641 gcgattggca attttatcga ccgtattcgt cttggctatg tagtagatat gttccagaca
    26701 gattttatga gctttccgat ttttaacgta gcagatgtga cacttgtatg tggagtgatt
    26761 tgtatgttaa tttatattat tttagatgaa aaggatcaaa gaaaaaaatg acagaactta
    26821 aaacgacgat tcaccatgaa tctggtagga tcgataaagt attgactaat ttattcaccg
    26881 attacagtcg ttcgcaaatt cagctgtggc tgaaaaatgg agcggtttct gtcaatggac
    26941 agccagtcaa agcaaattac aaagtaaaac aaggagacga gattactgtc ttgatccccg
    27001 aaccagaaac attagatgtg gtcccagaaa atatcccgtt gacgatcgtt tacgaagatg
    27061 aagatgtagc ggtgataaac aaacctcaag gaatggtcgt tcatccttca gcaggtcata
    27121 cgcatggcac aatggtcaat gcgctgatgt accatatcaa ggatctttct acgatcaacg
    27181 gcgttattcg tccaggtatt gttcaccgaa tcgacaaaga tacttccgga ctgctaatgg
    27241 ttgccaaaaa tgaccaggca cacgaatcat tagctcagca attaaaagat aagacctctt
    27301 tgcgcaaata tgttgcttta gtccatggag agattcctca cgaaaaagga accatcaatg
    27361 cgccaatcgg ccgttcgaag gaaaaccgga aaatgcaggc gataagagaa gatgggaagt
    27421 cggctgtcac acattttact gtattggaac gtttcgaagg atttactctg gttgaattaa
    27481 aactagaaac cggaagaaca caccagatcc gtgtgcatat gaagtatatc ggttatccgt
    27541 tagctggaga tccagtatac ggaccgaaga aaactttgaa aggcaatggt cagtttctcc
    27601 atgcgcaaat gttaggtttt acgcacccga ctacaaacga aaagatgatc tttgaagcac
    27661 ctcttccaga agtttttgaa aaaactttag aaaaattaag aaaaaacatt gcattttaat
    27721 ttagataacg gtatagtaat taacaacgaa agcataaaac ttaatagaat tcctttaagt
    27781 ttagtcccgt gaggctaaga agggtgggag caaggatacg tatggtgcgc cataagtaga
    27841 ttgctcattg acatagtata gctatcagtg tatcagtata cgctcagtct aaacattcct
    27901 cctgtcctct tagggcagga gtttttttat gcaaaaaagt agggggaatg aaaatgcaag
    27961 caaaagaagt agtagatcag gtaaccatga agcgagcatt gactcgaatt acttatgaga
    28021 ttatcgagag aaaccatagt atccaagata tcgtattggt aggtatcaaa acacgaggaa
    28081 tctacattgc atcaagaatt gccgaacgcc tgaagcagct agaagatatc gatattcctg
    28141 taggagaact ggatatcact ttataccgag atgacaaaaa agaaaatcca gaagaaccgg
    28201 aacttcattc ttctgacatc ccagtttcct tagaaggaaa agaagttatt ttgattgatg
    28261 atgttttgta tacaggacga acgattcgag cagcaatgga cgcagtaatg gattttggca
    28321 gaccaagaaa aatttcatta gcagtcctgg tcgatcgtgg gcatcgagaa ttgccaatcc
    28381 gtgcagatta tgttgggaag aacattccaa catctcgggc ggaagagatt ttagtagaga
    28441 tgcaagagct tgatggacaa gatcgcatca tgattttgaa agaggaagat tagaggagag
    28501 agaaacatgt cggagaaaaa aggatttcat aatgatgacg tggtgttaga tatccacgat
    28561 cgcccaaccc caggacattg ggtaggatta agtttacaac acttgttcac gatgttcgga
    28621 gcaaccgtgt tagtccccat cttagtaggt atcgatccag gtattgcgct agtcagttca
    28681 ggactaggta cattggtgta cttatttact actaaaggaa agatacctgc gtacttagga
    28741 agcagcttcg ctttcattgc agcgatgcag cttttgatga aaacagatgg ttatccagcg
    28801 attgcacaag gagcagtcac aactggatta gtctatttga ttgttgcgct gatcatcaaa
    28861 aagatcggtt cagcatggct ggataaaatc ttgcctccaa tcgtcgtcgg tccagtcatc
    28921 atggttatcg ggttgggact tgcggcaaat gcagcaacaa atgctatgta taatcagggc
    28981 acgtatgatt tcaagtatat tgctgtagcg ctgatcacat taggattaac aattttcttc
    29041 aacatgtggc taagaggttt tctcggattg attccgattt tactaggtat tattagcgga
    29101 tacatcgtag ccttgctgtt tggaatcgtg gatactaagc caatcgtaga cgctgcctgg
    29161 tttgctgttc ctaactttga gattcccttt gtacaatatg aaccaaagct atatcttgga
    29221 gcaatcacaa caatggcacc catcgctttt gtgacgatga cagagcatat cggccatctt
    29281 atggtattga ataaactaac taaacgtaat ttctttgaag aaccgggtct tcataagacg
    29341 ttgacaggtg atggattagc acaaatcgtg gcagcctttg tcggaggtcc tcctgtaaca
    29401 agttacggtg aaaacattgg tgttctggct atcacacgtg tccatagcgt gtttgtgatc
    29461 ggtggagctg cagtatttgc agtcatttta ggattcgtag gaaaattaag cgctttgatt
    29521 ttaagtatcc ctggaccagt catctcagga atcagtttta tcttatttgg agtaattgcc
    29581 gcaagtggaa tgaagatttt aatcgagaac aaaatcgatt ttgataaaaa gaaaaatctg
    29641 ttgattgctt cagttatctt agtagtagga attggcggac tcgtctttga agtaggcaca
    29701 tttacattgt cggcaatggc attagcaact gtacttggca tcattttaaa tcttatctta
    29761 ccagaaactt cacgaagcga agaaaactaa ggaggaaaca ttatgatcat caaatcagaa
    29821 cgtatcagct taaagcattt attgaccgtt gaggcactta ctgatcaaga agttatgggc
    29881 ttgatccgca gagggcagga attcaagcaa ggtgctaatt ggactccgca aaagcgccag
    29941 tactttgcta caaacctctt tttcgaaaat agtactcgca cacataaaag cttcgatgta
    30001 gcggagaaaa aactagggct agaagtgatt gaatttgaag caagcacaag ctctgtccaa
    30061 aaaggggaaa cactatacga tacagtactg acgatgtcag cattaggcgt agacgtagcg
    30121 gttatccgtc atggtgatga gaactattat gacgaactga tacaaagtaa aacaatccag
    30181 tgttcgatta tcaatggagg agacggaagc ggacagcatc caacccaatg cttgcttgat
    30241 ttgatgacca tttatgaaga atttggtcat tttgaaggtc tgaaagttgc catcgtaggt
    30301 gatatcactc actctcgcgt agctaaatcc aatatgcaga tgttaaaacg tctaggcgca
    30361 caagtattct tctcaggtcc tagagaatgg tacgacgatg aattcgaagt gtacggacat
    30421 tatatgccat tggatgaact actagatcaa gttgatgtca tgatgatgct tcgggtgcaa
    30481 cacgaacgcc atgatggaaa agaaagtttc tcaaaagaag gctaccatca ggaacatggg
    30541 ttaacagttg agcgtgcgaa gaagttgcaa aaacatgcaa tcattatgca cccagcacca
    30601 gttaaccgtg atgtagaatt agctgattca ttagtggaag gattacagtc gcggatcgtt
    30661 cagcaaatga gcaatggagt attcgtcaga atggcaatct tagaagcagt gctaaatgga
    30721 aaagcgtaat tataggagga tgaaaaaatg aaaacactca ttaaaaacgg tcagatcaat
    30781 acacgaaaaa atatgacgac accagcagaa atttggatag aagatgggcg aatcaaagca
    30841 atcggtacag gattctcaga agccgaattt gatgaagtgt ttgatgcaaa gggacaattg
    30901 atcacacctg gtttagtgga cgtccatgtt catttgagag agccaggatt tacatacaag
    30961 gagacgatcg aagctgggac tcgctcagct gctcgtggcg gtttcactac tgtatgcgcg
    31021 atgccgaatt taaatcctgt acctgatacg gcagaaaagc tgagacaagt atatgacatc
    31081 attagaaaag atgcagttgt gaaggtattg cagtatgcgc caatcacaga aaatctgcga
    31141 agtgagaaat tagttgatca agaagcactg attgaagaag gtgcttttgc tttcacaaat
    31201 gacggtgtag gtgtccaaac agctgggacg atgtatctgg ctatgaaaga agctgcaaaa
    31261 aacaataaag ccctagtagc ccatacagaa gatgaatctt tattatttgg cggcgttatg
    31321 cacgctggca agaaggcaga agaacttggt cttcccggga tcttaagcgt aaccgagtct
    31381 tctcaaattg ctagagatct gttgctagct gaagcaactg gtgttcatta ccatgtttgc
    31441 catgtttcta ctaaagaaag tgtccgtgta atcagagacg caaaaaaagc aggaatccac
    31501 gtaacagcag aagtttcacc ccatcatctt attttgatcg acgaagatat tcccgaagat
    31561 ttcggctttt ggaaaatgaa cccgccgcta agaggacggg aggacagaga agctttgatc
    31621 gaaggcttgc ttgacggtac gatcgattgc atcgctacag accatgcccc ccatggatta
    31681 gaagaaaaaa gccaaagttt catgaaatca ccatttggta tcgtaggaag cgaaacagcg
    31741 tttcaactga tctatactca ttttgtagaa actggtcgat tcacgttgga acaagtgatc
    31801 aattggttgg cagttaaacc ggctgagatt tttggtttga atgcaggaac gctaacggtt
    31861 ggtgcccccg ctgatgtagc agtttttgat attacacaaa cttgcacgat cgataaagaa
    31921 gactttttat caaaagggga gaatacacct tttatcggct ggaaagtaaa aggcgaaaca
    31981 caaatgactt ttgtcaacgg aaaacttgtg tggcagaagg gagaataaaa gtatggaacg
    32041 tttgcttata ttagaagatg gaacagtttt cgaaggaaag gcttttggtg cagatgctag
    32101 tgtcttcgga gaaatcgttt ttacaacaag tatgactggt tatcaagaaa ctatcactga
    32161 tcaaagtttt aacggtcaaa tcattacatt tacctatcct atggtgggta attatggggt
    32221 aaatagagat gattatgaat cgatttctcc aacatgcaaa ggagtagtag taaaagaaca
    32281 tgcacgtgta gcttcaaact ggcgaaacca aatgacgcta gattcatttt tgaaacaaaa
    32341 aggaattcct ggaatatcag gtatcgatac gcgcgccctt actcgcaaaa tccgtgaagt
    32401 cggaacgatg aaagctagta tagttgatgc tggagattcc ttcgaacatg catttgatca
    32461 gctgaaagca accgttctag caaccaatca agtgcagcaa gtctcaacta acaaacctta
    32521 tccaagtcca ggaacaggtc ggaaagtcat tgtcattgac ttcggcttga aacatagtat
    32581 tttacgagaa ttgagtaaaa gaaactgtaa cctcacagta cttccctata acacagatgc
    32641 cgagacgatt ttagcgctat caccagacgg agtcatgctg acgaatggac ctggtgatcc
    32701 aaaagatgtg ccagaagcaa tcgaaatgat caaaaaaatc caaggaaaag taccgatttt
    32761 tggtatttgt ctcggtcacc agttatttgc tttagccaac ggtgcagata cctataaaat
    32821 gaaattcgga catcgaggat taaatcaccc ggtacgagaa gtagcgaccg gaagaattga
    32881 ttttacttct caaaatcatg gctatgcagt cgatgaagcc acgattgatc cagaaaaact
    32941 catcgtaact catgtagaag taaatgacgg aacagtagaa ggagtcaaac acagagatta
    33001 tcccgctttc agtgtgcagt ttcatccaga tgctgcccct ggtccgcatg atgcactaca
    33061 tttatttgat gaatttatgg aaatgatgga cgcaggaaag gagcagtaaa cagatgccga
    33121 aacgtacaga tatcaagaaa attatggtga ttggatctgg tccgatcatc attggtcaag
    33181 ccgcagaatt tgactacgca ggaacacaag cctgccttgc cttaaaagaa gaaggatatg
    33241 aagtggtttt agtaaattct aatcctgcga cgatcatgac ggataaagaa gtggcagatc
    33301 aagtatatat cgaaccgatc acacttgaat ttgtatcaag gattcttcga aaagagcgtc
    33361 cagatgctct ccttccaact ttaggcggac agacaggcct caatatggca atggagctgg
    33421 cagcatcagg tattttagaa gaattgaaca tagaacttct aggaacaaag ctttctgcga
    33481 tcgatcaagc agaagatcgt gatctattca aacagttgat gaaagaattg aatcagccaa
    33541 tccccgagtc tgaaatcgtc aatacagtag aagaagctgt agattttgca aataaaatcg
    33601 gctatcctgt catcgttcgt ccagccttta ctttaggcgg aactggtgga ggtatgtgtg
    33661 aaaatgaaga agaattacgt atcgtatcag aaaatggatt aagtctttcc cccgctaccc
    33721 aatgtttgat tgaacgaagt attgcaggtt ttaaagaaat tgaatatgaa gtgatgagag
    33781 attcggctga taatgcaatc gttgtctgca atatggaaaa ttttgacccg gtgggtatcc
    33841 atacaggtga ttctatcgta tttgccccta gtcaaacatt gtctgaccat gaatatcaaa
    33901 tgcttcgtga tgcttctctt tcgattattc gagcacttaa gatcgaggga ggctgtaatg
    33961 tccaactcgc tcttgatcca catagtttta attattatgt gattgaagtc aatccgcgtg
    34021 tttctcgttc atctgcgtta gcaagtaagg cgactggcta tccgatcgct aagctagctg
    34081 ctaaaatagc gcttggatta acattagacg aaatgaaaaa tcctgttaca ggaacgactt
    34141 ttgcagaatt tgaacctgca ttagattatg ttgtagctaa aattcctcgc tggccgtttg
    34201 ataagtttga aaacggagaa cggcttttag gcacgcagat gaaagcaact ggtgaagtga
    34261 tggctatcgg tcggaatatc gaagagtctt tattgaaagc tgtgcgttct ttagaaatcg
    34321 gtacacacca tatcgaactt cctgagttgg cagacatcac ggacgatgac ctaatccaaa
    34381 aagtggttcg agcacaagat gatcgcctgt tttatgtagc tgaagctatt agaagagggt
    34441 acaccataga agaattagcg gatttgacaa aaatcgattt attcttttta gataaactgt
    34501 tgcatatcgt agaaatcgaa cagcagttga aacagcagcc gttggatgac aagatattga
    34561 aaacagcgaa acaaaacggt tttacagaca aaaaagtggc ggagctgtgg gaaataaacg
    34621 aagaagccgt tcgccagatg agaaaagaaa atgatttgat tcctgtatat aagatggttg
    34681 acacttgtgc agcggaattc gaatcaaaaa caccttattt ttatagttcc tatgaatatg
    34741 aaaatgaaag ttcacgatca gaaaagccct caattctagt actgggatct ggtcctatcc
    34801 gtatcggaca aggggttgaa tttgactatg cgactgtgca ttctgtcaaa gcaatccagc
    34861 aggcaggata cgaagcaatc atcatgaaca gcaatcctga aacagtttcg actgactttt
    34921 ctatttcgga taaattatat tttgagccat tgaccttcga agatgtgatg aatgtgatcg
    34981 agctagaaca gccgatagga gtaatcgttc aatttggtgg tcagacagca atcaacttag
    35041 ccgaaccttt gactgctgct ggtgtaaaaa tcttagggac aacgatcgaa gacttggatc
    35101 gtgcggaaaa tcgtgattta ttcgagcaag cgctgaaaga gttggatatt cctcaacctt
    35161 taggggatac tgctacaagt aaagaagaag ccgtggttat cgcttcgaga attggttatc
    35221 cagttcttgt tcgtccaagc tatgtattag gcggacgtgc gatggaaatc gttgaaaacc
    35281 agcgagattt agaagattac atggaacatg ctgtgaaagc atctccagaa catccagtac
    35341 tagtggaccg ttacttgctt ggtggtgaat gcgaagtgga tgcgatttgt gacggtgaaa
    35401 cagtactcat cccagggatc atggagcata ttgagcgtgc tggtgtccat tcaggggatt
    35461 caatggctgt gtatccacct caaacattat ctgaatcgat caagaaaacg atcgaagatt
    35521 acacgatccg cttagcatta ggtttgaatt gtgtcgggat gatgaatatc cagtttgtca
    35581 tccatgaaaa tgaggtttat gtgatcgaag tcaatcctcg agcaagtcgg actgttccat
    35641 tcttaagtaa ggtgacagga attccaatgg cacaaattgc gacgaaagct attttgggag
    35701 agaaactaaa gacacttggt tatgaaaacg gactttatcc agaaacaaaa gaagttcatg
    35761 ttaaagcacc tgtcttttct tttacgaaat tgcagaaagt tgatacttac ttagggcctg
    35821 aaatgaaatc gactggagaa gtaatgggat ctgacagaca attagacaaa gctttgtaca
    35881 aggcattcga agcttctgga ttacacttgc ctgaatatgg agccgtatta tttacgattg
    35941 cggacgaaac aaaagaagaa gcactagaat tagctaaacg ttttacagaa attggctata
    36001 gtctgatagg aacgaaacat acagctgctt atttcgaaaa aaatggtctg gtcgttgaaa
    36061 cagtagcgaa aatttctgag gaagcagcag aaaaaaatgt agtcgactta ataagagatg
    36121 gtaaaacaca agtggttgtc aatacgattg ataaagatcg gggaaatgct tctaaagacg
    36181 gtttcatcat ccgaagagaa gcagttgaac atggtattcc attgtttact tctttagaca
    36241 cagcagatgc gatcgttcga gtgatggaat cccgtgcttt ttcaactaac cctatttaac
    36301 ggcgagcaca tgttaagaca aaaagacgaa aaaccgttgc aggaggggac agatgaaaca
    36361 agaaatgatg gaaatcgttc agcaaaaaca gctggcacca aagatttttg aaatgacatt
    36421 gaaaggtgat ctagtaaaag aaatgcagca accaggacag ttcttgcata ttctcgtgcc
    36481 gcgtgcagac ttgctattac gccgaccgat tagcttgaat catatcgacc acgaaacaaa
    36541 tacttgccgt atcatttatc gggtggaagg tgaggggact agagtcttct ctactctttc
    36601 tgccggagac catttagatg tcatgggtcc tttaggcaat ggttttgatt tgtccaatct
    36661 tcatacagga gacgaagtat ttataatcgg tggaggaatc ggtgtccctc ctctatatga
    36721 gttatcaaaa cagttagtcg aaaaaggaat taagccaacg cattttttag gttttgccac
    36781 acatgaagtc gtttattatg aaaaagaatt taatcgtcta ggagaaacaa gaatcgctac
    36841 tgacgatgga acatacggta tgcacggaaa tgtcggtaat ttattgttag gaacgaagac
    36901 ggaacctaca gctgtttttg catgtggttc aaacgggtta ttgaagacag tggaacagct
    36961 gttttcttcg catcaaaatg tccagttatc attagaatcc cgcatggcat gtgggatagg
    37021 ggcttgctat gcttgcgttt gtcatacgcc ggaagatgaa acaggaacaa aaagtgtgaa
    37081 agtttgtgac gaaggaccga tttttaaaat aggagaggta attatatgaa tgagaatcct
    37141 ttagcggtca aacttcctgg cctagaatta aaaaatccta tcatgccggc aagcggctgt
    37201 ttcggttttg gcaaagaata cggaaaatac tatgatttga atcagcttgg atcgatcatg
    37261 gtcaaggcaa cgactccaaa tgcccgcttt ggcaatccca cgccaagagt ggcggaaaca
    37321 ccatctggca tgttgaatgc aattggtttg caaaatcctg gtctagacgt tgttatgcat
    37381 aaacttcttc cagaattaga ggagtacaat gagttaccca tcatcgcaaa tgttgctggt
    37441 gcttgtgaag aagactatgt agaagtttgt agcaaaattg gcgaagcacc aaatgtgaaa
    37501 gcaatcgagc tgaacatttc ttgcccaaat gtcaaacatg gcggaattgc ttttggaaca
    37561 gattctgatg ttgcttttca attaacacaa gcagtaaaga aagtttcttc tgttcctgtc
    37621 tatgtcaaat tgtccccaaa tgtaacagac atcgttccaa tcgctcaggc aatcgaagct
    37681 ggaggggcag atgggtttac gatgatcaat actcttcttg gtatgcggat tgaccttaag
    37741 acaagaaaac cgattttagc caatcaaaca ggtggactct ccggaccagc aatcaagcct
    37801 gtcgctattc gtttgattca ccaagtagca agtatatcca gccttccaat cattgggatg
    37861 ggcggcgtgc agacagtgga tgatgttctt gagatgttta tggctggagc aagtgctgta
    37921 gctataggga ccgcgaattt caccgatccg tatatctgtc ctaaactaat taaagagctc
    37981 cctgtgcgaa tgagcgaact aggcatcgaa tcgctggaaa gattaatcaa agaagtgaga
    38041 gaggaacgtg aacgatgaat caactagacc aacgaccaat tattgcatta gatttttcaa
    38101 ctaggcaaga ggtagaagac tttcttcgat ttttcccgaa agaagaaaaa ttgtttgtga
    38161 aaatcggaat ggaattgttc taccaagaag gacctgaaat cgtacgttat ctaaaagagg
    38221 ctggtcataa tgtattttta gatctgaaac ttcacgatat tccgaatacg gtcgaaaaag
    38281 caatgcgggg attggcaaag cttggtgttg atctcacctg tgttcatgcc gctggcggta
    38341 ttcgtatgat ggaggcggca atgcgaggat tagaagaggg gactccagaa ggtggaaaac
    38401 gtccgctact tctagctatc actcagctta cttctacaag tgaagaagaa atgcatgccg
    38461 atcaactaat cgaagtttct ttagaaaaaa gtgtgatcca ttatgctagc tgtgccaaaa
    38521 aagcaggatt agatggtgtg gtttcttctg cttgggaagt agaagcgatc aaagaaatgg
    38581 caggagacga atttgtttgc ttaactccgg gtataagacc agaaggaaca gtcgcaggag
    38641 atcagacaag agtagtcaca ccttctcagg caaaaaagat cggctcaact tttatcgtta
    38701 taggtcgacc aatcactcag tcaactaatc cttataaagc atatcaaacg attcaaacag
    38761 aatggagcta acctaaaatg aatgttgaac aaagtatcgc aaaagactta ttagaaattg
    38821 aagcagtatt tttaaatcca agtaatcctt ttacatgggc aagtggaatc aaaagcccaa
    38881 tttactgtga taaccgaatc acgatgagct atccaaaagt ccgaaaagag atcgcaaaag
    38941 gattagcaag caaaatcaaa gaagcttacc ctgaagtaca agtgattgct ggaactgcaa
    39001 cagcaggaat ccctcatgcc gcatgggtag cggaaatact ggatttgcca atggtctata
    39061 ttcgaagtaa ggcgaaagat catgggaaag gtaaccaaat cgaaggacgt atatttgaag
    39121 gtcagaagat ggttgtgatc gaggatttga tttctacagg cggaagtgtc ttggaagcag
    39181 ctgaagcagc aaaaagagaa ggtgcggata tccttggtgt tgcggctatc tttacgtatg
    39241 agttgccaaa aggaaaagca aatttcgaaa aagccgaaat ccctcttatg acgttgacca
    39301 attattctgt attgattgaa gcagctttag aagatcgtta tatcgatgag caagaattaa
    39361 ctctgctaaa agaatggaaa aaagatcctg aaaattggca gatttaagcg cgcttgatga
    39421 agatagagaa atccgatttg tgtctccaga atcatcaatt ggaatagcaa aaatccaaaa
    39481 actgattgcg agaagcatct tttcatcagg tatgaaaaga aacttctatc gtgatcagtt
    39541 tttgattttt ttaaaattca tttttctaag actggttagc agaaagaaga gaataatctc
    39601 ttctcttttt tctattatat atagattttc tctatttttt ggtaagggag ctgtttcaaa
    39661 aatggtaaag agcggaaatg ttgcgaatat cagttattta atgatatgat attcggaaaa
    39721 agaaatctca aagggaggta acttttatgg ggttgaatga atttatttta ggattgaaca
    39781 atttagagac gatcacaagg gcacctggat tttttaaata tacagagcat acagttgcag
    39841 cacattcgta tcgtgtagca tctattgcgc aagttctagg agacattgaa gaagcgaatg
    39901 gacaaccgat cgattggaaa ctgctctatg aaaaatcatt gaatcatgac tacacagagc
    39961 gttttattgg tgatatcaaa acgccggtga aatacgcaag caaagagtta agagggatgc
    40021 tgcaaacagt tgaagaaaag atgacggacg aattcatcaa ccaagagatt ccagctgagt
    40081 tccaagaagt ttatcgccgt cggttgttcg aaggaaaaga tgaaaccgta gaaggagaac
    40141 tattagccat ttcagataag gttgaccttc tttatgaatc ttttgatgaa atcatcaaaa
    40201 ataacccgga aaaagtttat aaggagatgt tcattgaggc agtgactacg attaaaagct
    40261 ttgaacatcg gccttctgtt cagtatttct ttaaagagat atttccggaa ctattgaacc
    40321 aggaatttta tggaaaagag gcgtttttag aaaccgtctc aacgattgta aagaaagaag
    40381 aaaaataaaa atgatatgag atgcaggggg aagtatagtt gaaaaagaat atggatgtcg
    40441 aatggggcta ttctggagac aaaggtccgg aaaattgggc gagtttatgt gattggtttg
    40501 cgagaggtgc ggaatttcca ttgcagtcgc ctattcattt gaccaagggg gaagaaacca
    40561 aaatagaagg agacacgctt tcctttcatt atcaaaagca acggtttact gacaaggaat
    40621 tcaaaaacac gattcattta gtaccttttg accaactcag ttatgtagag ttcaagaaag
    40681 aacgctatta tttgacagat attcatttcc acttgccttc tgaacatatc atcaacggaa
    40741 aacaggaaga tattgaattt cattttgttc atatgaacag caaaaaagag aatctagttg
    40801 tgggtgtcat gtaccagcta atggtacagg aaggttggct gtataatcaa gaaaatgggg
    40861 aaagttggaa cttagaaaaa catgagcatt gggtaaatcc ggatgtgttc tttccggagc
    40921 aaaaaagcca tttccattat attggctcat tgacaactcc gcctaccagc gggccaatcc
    40981 aatggtttgt aatggatcat gtaggaaaac tgaaccaaga gtttcttctc cccttcgatg
    41041 gtcagtatgc tcgaccgaac aaccgaccaa tccagccctt gaatggaaga gaaatttatt
    41101 atttcgagga gtttgagcaa tgaatattca gcagatgaaa tacgtggtcg ctgtagcaaa
    41161 caatggtagt ttcagagaag cggcaaagaa aatgtttgtc tcccagccaa gtctttcagc
    41221 tggtatcaaa gaattagaaa ccgagttagg gatcacgctg tttactcgaa cgaatcgagg
    41281 ggcatattta acagaagaag ggcaagactt cttaaatcgt gccgagaaaa tattggtcca
    41341 gcttgataca ttagaaactc actatctaac gagtgaaaag agagacaatt tttcgatcgc
    41401 tgcccagcat tatgatttct taggtccttt gaccgcacag ctactcgagg aattcaaaga
    41461 cgagatcaaa caatttcgga tcatggagac gacaacagcg agagtgatcg aagaagtcaa
    41521 agagcagcac agtgagatcg gcattattta tatgaatgag cataatcaag ctggtatcag
    41581 aagatattta caacaaggag agctaagtgg ccgcagcatt ggaaaattca aaacgcatat
    41641 ttttttaagg caaggacatc cattagctga acacgcagag atcaccaaag aagaattgtt
    41701 atcttatcct caagttcgtt ttacacagga tggaaataat ttcccgtatt tttatgaaga
    41761 ccttatcgag atccctgatc aagaaacagt gatttatacg agtgatcgag gtactttgat
    41821 gaatatcgtt cttgaaactg atgcttacgc ttcaggatct ggtattgtga ttggagggat
    41881 tcgcgagcac ttgcggttaa tccctttggc agaaggcgaa ctaaatgaat tttatatcat
    41941 ttattcggcg aaacgtccgc tcagtgaaat cgctcaaaga tttatcaaag agcttactcg
    42001 gttactggac cagcagagct aagaaaagca gatgagaacg ctgtttattg aacaccgttg
    42061 atcaaaaaag aggggttgtg acagaatttt gtcacaactc ctctttttgt agcaaacaat
    42121 ttataactaa ttgctgttgt gggaaagtac gcaaatatga ttctagaaac actcattgat
    42181 tacctgtttt gtttcatagc tgtaattttt tcttcttctg gtgtcacgat aatcgtcttt
    42241 tggttttcat aaataacata accaggcttg gcgccatttg gttttcggat atgctttacc
    42301 tgtacaagat caaccggcac ttgggcagag tatcggtatt ttgaaaaata ggcagctaac
    42361 tcggcagctt ctgtaattgt ttcatctgag ggcttgtcac ttttaatgat gacgtgcgaa
    42421 ccgggtatat tttttgcatg aagccaatag tcggtttttt tagctgtctt cattgtcagc
    42481 tggtcatttt gaagattgtt tttaccaaca agaatgagcg ttccgtcaga agaaaagtat
    42541 tgatcaggct gagatggttt cttacgtttt tgttttttta agttcttctt tttcaggtat
    42601 ccttctgaag tcaattcttc tttaattgct tcgatatcca ttgggccggc aatttccagt
    42661 tgagataaga cagattcaag atattggatt tcatctttgg cttcttggat ttgttcgcca
    42721 atcagcttaa cagcgttctt cagcttttgg tatcgatgga aatatttctg tgcattttga
    42781 tttggtgtca atgccggatc tagtgctatt ttgattggcc gatcttcttc ataataatta
    42841 ggtaaaacaa cttcttttgc gccgcgtggt acttgtgtca gaaaagtagt aagtagttcg
    42901 ccatttctac ggtaattctc agcgttttcg gattctttga gtgtttgttc acgttttttc
    42961 aatttatttt tatttcgttt tagttcattt tcgatttttc gaatcagttc tccgccttgt
    43021 tgttttgccc gatctttctc tgctttttcg tgataatagc tatctaataa ttgactgagt
    43081 gttggataag ctgttacagc agatgcctgt tcggacaata cctgataagc aatcggtgtg
    43141 aaaaaatctt tattttccgt ttcgtaaaaa gtaggaatag gctgatggct gatggctgag
    43201 aaaaattcgt cccatacaac catcttttca ttaggacgtt ccagtaatct tttggtcagc
    43261 tcttgagcga tatcgaaacc gattccctga aactgacgtt ggattgcttt tggatctagt
    43321 tcagtttggg aaagccgctg aaagattttt tctttctcgg agctaaatgg gttcaagacc
    43381 tcttgttttg gcggagcaac atattctaca ccaggaagca aagaacgata agtgttttga
    43441 gagcttccaa tgtgtttgat cgcatccagt atttttccag tttctcgatt aactaagacg
    43501 atcgtactat gacgtcccat taattcaacg atcaatatga tattttgtag atcacccaac
    43561 tcgtttcgtt tagcaaagtg gaaatggatc actcgatcat tttcgatttg ttcgatgtct
    43621 tctaaaatag cgccatccaa atattttcga agcatcatca cgaagttagg cggattatca
    43681 ggattctgat aatcgatttg ggtaatctgt acacgagcat aacttgggtg cgcagataat
    43741 aataatcgat ggtttttgcc gcgtgaacga atcaccagca cgatttcatt ttcataaggc
    43801 tgatggattt tcgagatccg gccagatagt aacgtttcac gcaattcgtt gaccatcgcg
    43861 tgggtaaata caccatcaaa ggacatggtt cattcctcat ttcttttcat aagtatattc
    43921 attataacga aaaaaagaag aaaaaccaat ttggccgaat agtaaagcaa aagtctagaa
    43981 aaatcttgga ctaattacat aaaaaacagt gtttctatcg ttgaaatcca aatttttagg
    44041 t
//
LOCUS       DACDRN010000015        41302 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDRN010000015 DACDRN010000000
VERSION     DACDRN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.015"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            84..938
                     /locus_tag="IWJ80_001059"
     CDS             84..938
                     /locus_tag="IWJ80_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP9460807.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            939..2276
                     /locus_tag="IWJ80_001060"
     CDS             939..2276
                     /locus_tag="IWJ80_001060"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP9460808.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            2281..2505
                     /locus_tag="IWJ80_001061"
     CDS             2281..2505
                     /locus_tag="IWJ80_001061"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP9460809.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            2507..3397
                     /locus_tag="IWJ80_001062"
     CDS             2507..3397
                     /locus_tag="IWJ80_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9460810.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            3406..4224
                     /locus_tag="IWJ80_001063"
     CDS             3406..4224
                     /locus_tag="IWJ80_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP9460811.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            4331..4780
                     /locus_tag="IWJ80_001064"
     CDS             4331..4780
                     /locus_tag="IWJ80_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9460812.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            4798..6480
                     /gene="recN"
                     /locus_tag="IWJ80_001065"
     CDS             4798..6480
                     /gene="recN"
                     /locus_tag="IWJ80_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP9460813.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(6535..6651)
                     /locus_tag="IWJ80_001066"
     CDS             complement(6535..6651)
                     /locus_tag="IWJ80_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP9460814.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(6724..7680)
                     /locus_tag="IWJ80_001067"
     CDS             complement(6724..7680)
                     /locus_tag="IWJ80_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9460815.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            7851..8219
                     /locus_tag="IWJ80_001068"
     CDS             7851..8219
                     /locus_tag="IWJ80_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP9460816.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            8389..8820
                     /gene="mraZ"
                     /locus_tag="IWJ80_001069"
     CDS             8389..8820
                     /gene="mraZ"
                     /locus_tag="IWJ80_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP9460817.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            8841..9800
                     /gene="rsmH"
                     /locus_tag="IWJ80_001070"
     CDS             8841..9800
                     /gene="rsmH"
                     /locus_tag="IWJ80_001070"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP9460818.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            9805..10206
                     /gene="ftsL"
                     /locus_tag="IWJ80_001071"
     CDS             9805..10206
                     /gene="ftsL"
                     /locus_tag="IWJ80_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP9460819.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            10207..12399
                     /locus_tag="IWJ80_001072"
     CDS             10207..12399
                     /locus_tag="IWJ80_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9460820.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGMFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            12425..13387
                     /locus_tag="IWJ80_001073"
     CDS             12425..13387
                     /locus_tag="IWJ80_001073"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP9460821.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            13401..14762
                     /locus_tag="IWJ80_001074"
     CDS             13401..14762
                     /locus_tag="IWJ80_001074"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP9460822.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            14788..15876
                     /gene="murG"
                     /locus_tag="IWJ80_001075"
     CDS             14788..15876
                     /gene="murG"
                     /locus_tag="IWJ80_001075"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9460823.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            15891..17111
                     /locus_tag="IWJ80_001076"
     CDS             15891..17111
                     /locus_tag="IWJ80_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP9460824.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            17267..18592
                     /gene="ftsA"
                     /locus_tag="IWJ80_001077"
     CDS             17267..18592
                     /gene="ftsA"
                     /locus_tag="IWJ80_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP9460825.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            18615..19856
                     /gene="ftsZ"
                     /locus_tag="IWJ80_001078"
     CDS             18615..19856
                     /gene="ftsZ"
                     /locus_tag="IWJ80_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP9460826.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            19873..20550
                     /locus_tag="IWJ80_001079"
     CDS             19873..20550
                     /locus_tag="IWJ80_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP9460827.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            20564..21163
                     /locus_tag="IWJ80_001080"
     CDS             20564..21163
                     /locus_tag="IWJ80_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP9460828.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            21178..21465
                     /locus_tag="IWJ80_001081"
     CDS             21178..21465
                     /locus_tag="IWJ80_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP9460829.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            21514..22296
                     /locus_tag="IWJ80_001082"
     CDS             21514..22296
                     /locus_tag="IWJ80_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP9460830.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            22317..23024
                     /locus_tag="IWJ80_001083"
     CDS             22317..23024
                     /locus_tag="IWJ80_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP9460831.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            23286..26084
                     /gene="ileS"
                     /locus_tag="IWJ80_001084"
     CDS             23286..26084
                     /gene="ileS"
                     /locus_tag="IWJ80_001084"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP9460832.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(26133..27659)
                     /locus_tag="IWJ80_001085"
     CDS             complement(26133..27659)
                     /locus_tag="IWJ80_001085"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP9460833.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(27674..28321)
                     /locus_tag="IWJ80_001086"
     CDS             complement(27674..28321)
                     /locus_tag="IWJ80_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP9460834.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(28505..28834)
                     /locus_tag="IWJ80_001087"
     CDS             complement(28505..28834)
                     /locus_tag="IWJ80_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP9460835.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            29011..29739
                     /gene="sfsA"
                     /locus_tag="IWJ80_001088"
     CDS             29011..29739
                     /gene="sfsA"
                     /locus_tag="IWJ80_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP9460836.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            29755..30768
                     /locus_tag="IWJ80_001089"
     CDS             29755..30768
                     /locus_tag="IWJ80_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9460837.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            30768..32045
                     /locus_tag="IWJ80_001090"
     CDS             30768..32045
                     /locus_tag="IWJ80_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9460838.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            32108..34810
                     /locus_tag="IWJ80_001091"
     CDS             32108..34810
                     /locus_tag="IWJ80_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9460839.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            34962..35279
                     /locus_tag="IWJ80_001092"
     CDS             34962..35279
                     /locus_tag="IWJ80_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9460840.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            35309..35629
                     /locus_tag="IWJ80_001093"
     CDS             35309..35629
                     /locus_tag="IWJ80_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9460841.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            35737..37197
                     /locus_tag="IWJ80_001094"
     CDS             35737..37197
                     /locus_tag="IWJ80_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9460842.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            37265..37486
                     /locus_tag="IWJ80_001095"
     CDS             37265..37486
                     /locus_tag="IWJ80_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460843.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            37517..37699
                     /locus_tag="IWJ80_001096"
     CDS             37517..37699
                     /locus_tag="IWJ80_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP9460844.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            37699..38112
                     /locus_tag="IWJ80_001097"
     CDS             37699..38112
                     /locus_tag="IWJ80_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP9460845.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            38235..39416
                     /locus_tag="IWJ80_001098"
     CDS             38235..39416
                     /locus_tag="IWJ80_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9460846.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            39805..39878
                     /locus_tag="IWJ80_001099"
     tRNA            39805..39878
                     /locus_tag="IWJ80_001099"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39839..39841,aa:Arg,seq:cct)
     gene            complement(39947..41086)
                     /locus_tag="IWJ80_001100"
     CDS             complement(39947..41086)
                     /locus_tag="IWJ80_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9460847.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT        13751 a         7035 c         8777 g        11739 t
ORIGIN      
        1 tctatgaatc atcataaacc tgttataaat cgggaaaatt ctatggtaga atgaaaacac
       61 aaaaaatatc tgggagtggt tagatggcag aattgattaa cggaaaagaa ttagctgaaa
      121 aaatgcaggc agagattgct gaaaaaatcc aaaaattgaa agataatggt atccatcccg
      181 gattagtcgt tttattggta ggggaaaatc cagccagcca aatctatgta agaaataaag
      241 aacgtgtagc aaaagaaatc ggaatccatt cattggtaga gcgttatcct gagacgattt
      301 ctgaagacga actacttgca gaaattgaaa aatacaatca agatcctagt tttcatggta
      361 tattggtcca attaccattg ccaaagcata tagatgaaga aaagatcctg ttagctatcc
      421 gccctgaaaa agacgtagac ggttttcatc cgatgaattt aggcagactc cttgcgggta
      481 aaccggacaa gattccttgt actccatacg gcatcatgaa aatgtttgaa gcttattcta
      541 tcgatccggc gggtaaacga gcagtagtta tcggacgaag caatatcgtc ggtaaaccga
      601 tggctcagct actcttgatg gcagatgcaa ctgttactgt tgctcattct aaaaccgaaa
      661 atctggctga actggcacaa gaagcagata ttttagtcgt agcaatagga cgtggacatt
      721 ttgtcacaaa agaattcgta aaaccaggtg ctgtagtgat agatgtaggg atgaatcgtg
      781 atgctaacgg taagctgatc ggtgatgtga aatttgatga agtggagcct attgcaggct
      841 ggatcactcc tgtgccaaag ggtgtagggc caatgactat cacgatgctg atgtatcaaa
      901 ccgtaaaatc tgccgaagct tttggagcag gtgaatagat ggcacaagaa tatttaactg
      961 tcactacatt aacgaagtat ttgaaaagaa aatttgatgc cgatccttat ttagaacgcg
     1021 tatatttaac aggcgaaata tcgaatttcc gtttacgtgc caatgcccat caatatttca
     1081 gcttaaaaga tgaccatgca aaaatatctg ctattatgtt caaaggagca ttccaaaaat
     1141 tacgattcca gccaaaagaa ggaatgaaag tccttgtcgt tgggcgtatt tcattatatg
     1201 aagcgggagg ttcttatcaa atctatgtag agcacatgga gcctgatgga ataggtgctt
     1261 tatatcaaga attggaagaa cgaaaagaaa agctgatgaa ggaaggcctt tttcaagggc
     1321 ctaaaaaaat gctgccacgt ttcccaaagc gaatcgcggt ccttaccagt ccaagcggag
     1381 cggttatccg cgatatcatc acgacggtga aaagaagata tccaattgtc caattggttt
     1441 tgttcccaac agttgtacaa ggaaaccaag cagcggacaa tgttgtcagg aatatccaga
     1501 gagtagaaga gatgggcaat ttcgatacaa tgatcatcgg tcgtgggggt ggttcgattg
     1561 aggatttatg gccgtttaat gaagagcggg ttgctcgggc gatttatcaa gctaatacgc
     1621 cggtgatttc atctgtagga catgaaacag ataccacgat cgctgattta gtagcagatg
     1681 ttcgggctgc cactccaaca gcagctgcag aattagctgt tcctgttttg tcagaagagt
     1741 tgatgcgcat tcaagaacgg caagctagat tgcagcaggc gtttgtacgc cagatccagc
     1801 gcaaacaaga acggttcgag cgtgctcgac gttcctatgt atttcggcag cctgaaagat
     1861 tgtatgaagg tcagaccatc aaattagacc agttgaaaca gcgattatat caagctacac
     1921 agcaaattta tcatgcgaaa gaaaaacaaa cgatcgcatt aagccaccaa ttggaacaag
     1981 tggctccgct ttatcgagtt aaagcagctc gccaagaaac tgcttatcta gaaaaacgct
     2041 tagaagagaa aatccaacaa tatatgaaac aacagcagca gcgttttgct caagctgttc
     2101 aatctcttga tttgttgagt ccattgaaaa tcatgggaag aggctacagc tatacgacgt
     2161 taaatgatca agtagtaaaa agtgtcaccg agattcatca aggggatttg ctgacgatcc
     2221 attatcaaga tggaatcgtt aaaggacaag tacaagagat agacaaagga gaataaaaat
     2281 atggaaaatc caacttttga agagtcactg caagaattag aaaaaattgt catgcagtta
     2341 gaacaaggag acgttccatt agaatcagct ctagattcgt ttaaacgagg aatggaatta
     2401 agcaaacatt gccaagatac attagctaaa gcagaagaga ccttgacgaa aatgatgaca
     2461 gaatcaaatg aagaagttga ttttgacgga aacgaggaaa gctgagatga gattaactga
     2521 cttttcagct attcatctac ctgctgtaga aaaagaaatc ttgtcttttt tagatgagca
     2581 tacaacggat agaggactct atgatgcgat gagttattca gtgaaagcag gcgggaaaag
     2641 aatacgtccc ttattacttt taacggccgt tgcttccttt gatgagccta ttgatgttcc
     2701 cgtctatcaa gtagcagctg ctttagaaat ggtgcatacg tattccttga tccacgatga
     2761 tttgcctgcg atggacaatg atgatcttcg tagaggaaaa cctaccaatc ataaagtatt
     2821 cggcgaagca ttagctatat tggccggaga tggtttgctt acaggagcat tccagctaat
     2881 cagtatggca cacttaggta attctcctaa gcttctgctg cttcagcaat tagctgtttg
     2941 tgcgggatca caaggtatgg ttgctgggca agctgcagat atcgagggcg aatcgaaaaa
     3001 gctttcttta gaagaattag catttattca tgaaagaaag acagggcatt tgattcgtta
     3061 tgccttgctg gctggtggca tattagcgaa gcaacctgaa gaaatcttat tgctacttca
     3121 acgattagcc gaacatttag gtttagcttt tcagataaga gatgatctac tcgatgtgat
     3181 tggtacaacg aaaacattag ggaaaacagc tggtaaagat gaacgtatgg aaaaaaatac
     3241 ttacccgcgt ttattaggcc tagaaaagac acgtgaggcg ttggagattg agctgttatc
     3301 cgccaacaag atcatagaca aattagaaga ggacgttttt tcttttgatg gcgggctgat
     3361 gcgccagatg atcaagcaat ttgatgtgga gaaatgaggg aaagaatgga aaaagaacgc
     3421 gtagatgtat tagcggtcaa acaaggatta tttgaaacaa gagaacaagc caaacgttca
     3481 gtgatggctg gtttgattta caacgaaaaa aacgagcggt ttgataaacc gggagaaaaa
     3541 attccagtga gtagtgaatt aagagtcaaa ggtaaaaaac ttccttatgt ttctcgaggg
     3601 ggattaaaat tagaaaaagc actcaaagca tttgatctat ctgttgaagg aaaaacattg
     3661 ttagatattg gtgcatccac tggcggattt actgatgcag ctttgcaaaa tggcgcaaaa
     3721 atgagttatg ctttagacgt tggctacaat cagctagctt ggaaaataag acaagatcct
     3781 agagtcgtcg taatggaaag agtgaatttt cgttatgcaa aacccgaaga ctttacctta
     3841 ggaattccag aagttgctgt tatcgatgtt tcttttattt cactaaaact gatgctgcct
     3901 ccattacatg ctattttaaa agaagaggga gaagtgattg ctttaatcaa accccaattc
     3961 gaagcaggca gagaagctgt agggaaaaac ggaattgttc gtgaccctca gacccatcaa
     4021 aaagttttag aagacatttt gtcatttgcg gcacaaggtg gatatgatgt gctggaatta
     4081 agttattctc cgatcacggg gggagaagga aatatcgagt tcttggctca cttgaggaaa
     4141 gtacccgaaa gcggaacaat caattcagct attaatatgg cagaagtggt ttcaaatgca
     4201 cacgaacagt ttgaccataa ataaattgta actgtgaaaa tcaagtgaga agtatgcgga
     4261 ttcttcttta ttataccaag gaaataagct atcattgatt aagaagaata tgctaaggag
     4321 cgaagacgta atgagaaaaa aagatcggca tcgtttgatt actcgattgt tgagtgagca
     4381 agatatccga aagcaggaag aatttgttga aatcctgaaa agcaaaggaa tatctgttac
     4441 tcaagctacg atttccagag atataaagga actgaaactg atcaaggtac cggcttcaga
     4501 tggcggctac cgttatagca taccagctga aacaaacgaa gatgtaaata ctaaattgga
     4561 aaaactatta aaagacgctt tcgtggcagt tgaccaaatg gaaaaatttg ttattttgaa
     4621 aacattgccg ggaaatgctt cggcagcagc aaatttgatt gataaacgtt acaagaaaga
     4681 attattttcg attatcaatg atgacgataa tgttttgatg attacacgaa ctgaagacca
     4741 ggcagtttta ttgaagaaag acttcctgca ttacctttaa gttgaggagc gaatgtgatg
     4801 ctacaagaaa tcagtattac taactttgcg attattccgg aattgcggct ttcttttcac
     4861 gaaggaatga ccgcgctgac cggagagact ggtgcgggta aatccattat tatcgatgct
     4921 ttaggtctgt tagcaggagg aagaggttca agcgattata tacgtcaagg agcagaaaaa
     4981 tgtgtgcttg aagggttgtt tgaactacca aaacaagaag gattctctga actgatggtt
     5041 gaattaggga tcgaaacaga tgaggacaat ctgattgttc gacgggatat gtccttaact
     5101 ggaaaaaatg tttgtcgagt gaatggtcat attatcacat tagctaatct gagaagaatc
     5161 ggcagttatt tagtagatat ccaaggacaa aacgaacatc aggaactgct ccagcctgaa
     5221 tcacatcttg ctttattgga tcgttttgga gatgtagctt tccaacagaa gaaaaagagt
     5281 tatcagcaag aatatatctc ttatcgtgag ctggaaaaaa gggtacgaaa agtgcagcaa
     5341 aatgaaaaat cctatgttca gagaatagat atgctccatt tccagcaaga agaaatagct
     5401 gctgctgatt tgcaagtcgg agaagaagag aaattaaaag aagaaagaga aaaacttagc
     5461 aattatcaaa aaattgctga tggattagct gcaggttacg gagcactgac gaataatgaa
     5521 caaaacagtg tagatggtgt tggcttggcc gtttcagaac tgcaagggat cgcccatcta
     5581 gatgtggagt atgaagcaat ttatgaaaat atccaaagtg cttactattt gctgcaagat
     5641 gcaatcggcg atatgagtcg tcagattgat ttattggagt tagatgagag tcgtttagaa
     5701 gaagttacgc aacgtttaga attaatccga cagttgaaac gtaaatacgg cgaatccatt
     5761 gagtcaatat tggcttacta tgacgaaatc accgaagagc tagcttcttc tgatttttca
     5821 gaaggtcaat tggacaaaat gaaagaggaa ctagagcaaa aagagcttct acttcagcaa
     5881 caagccgcag atcttcatga agcgcgtaaa aaaatcgcaa aagaattgga aaaatccatt
     5941 ctgcatgaat tgaaaagtct gtatatggaa aacactgagt ttgaggtacg tttcttaaaa
     6001 gaagaaaata gacaattgaa tcatgatgga tttgaccaaa tcgaatttta cattacgacc
     6061 aatccaggag agccactgaa gcctttggtg aaagtcgctt ctggtggcga actgtctcgg
     6121 atgttgcttg ctttgaaaac tattttttct tctgagcaag gtgtgacaag tattattttt
     6181 gatgaagtgg atacaggtgt gagcggaaga gttgcacagg ccatcgcaga taaaatttct
     6241 caaatatcga aatactctca agttttgtgc atcactcatc tgcctcaagt tgctgcagtc
     6301 gccgactatc aatattatat tgtgaaagca gtgattgatg gacgtacgca aacttctgtt
     6361 tcagagttga aaacaaaaga aagagaagaa gaaattgcac gtatgctggc tggaagtgag
     6421 attacaaaac tcacactcga acatgcaaaa gaactattga aactcgcaaa aaggacctga
     6481 gccacctcgc agtggttcag gtccttttca gcagctgata gctatatttt atttttacat
     6541 gacactcatt acagcagtca atacaacaga tccaatcgtt aaaatcagca ttgtccaaat
     6601 gacaaatttt gttactttag caaaagtact tgtttgtttt ttttcacgca ttgttttcaa
     6661 cctcgtcatt aattagttga ttcaagtgta caatcttttt tccatttcca caagagggct
     6721 tttttataaa aacttccctt tttttaaata ataggtagtt acgatacaaa taaataatgt
     6781 taatagaact agccatataa aggcatcatc cctttcagca ataggtaact taatgttcat
     6841 tccatagata ctgccgacga ttgtcggtat agttaaaata atcgttaaag aagttaaaat
     6901 tttcataata ttattcagat tattcgatac gattgctgaa aaagtctgat tcatttgttc
     6961 tactagtttt aattgaatgc gagaagtagt cattgcctgc tctgtttcca ccaatacatc
     7021 acgtaaatga tttttcaaag catgattttc ttggaaaagt ttattttgcg ctagttcttt
     7081 caatgcatct aaattagcag aagtcgcttc tctgaaataa acgaggcttt tttggatatc
     7141 catgatttga taaagttgtt tgttttcagt ggatacttga agtttggctt ccaaagtatc
     7201 gcattgagat tgaagcgtag ctaaataact gttgtaagag agtgtgacct gccaaatgaa
     7261 ttgaaagaag atattgagat taggcgaaat atcacttttt ggaaaggtct ggctaaagac
     7321 tttctttaaa aacagtggtt catgattcgt aattgtgata agcttctttt tgggcgtcaa
     7381 aataatagag agcgggtatg tatccacttg aaaataaccg ctaggacttg ttttagcaaa
     7441 aggatattgt aacaataata acgctggctt tgtcaacttt gattgattga attcttctgt
     7501 acgggaattt tctgcatcgt ccaatatgga agtgatgtaa tctgacggaa tttcaaattg
     7561 atgtgtcagc tgatgaatct cttcttgatt aggacgttct acacaatacc agatcgtttg
     7621 gtcagtttcc tttccggtat tacgcagttt ctcattatct aatgtaaaat atttgatcat
     7681 gattttcctc ctgttgcctg tttcttttat tataacggat agggaattgg aatacatgga
     7741 atgtgtttgt aagatataga atataactgc ataaatttaa ataaaatgaa acagttgtgt
     7801 aaagtatata cagtatactt gaagcaacga taaaaaagga gtttacgttc atgggcacat
     7861 tcacaccact tatcttgttt tggtatattt acccggtaat cgttttgttt ggttgccaat
     7921 ttcttgtaaa attattttcg ttaaatcgtc gttttaaaat aaaagcccct gatttggcga
     7981 ttcctttttt gtggggcggt atgcatgcat tgtctagaaa tacagggaca atttctatct
     8041 taccgttttt ctttatttct gttttgctaa tgggaatttg tattgctgtt ttccaagcat
     8101 attattatga agaaattatt tatccgcgtt atttcaagat gacttggcgt ttggtttttc
     8161 tgttgacaat cgtcttgtac gctgtcttga tcattgtgaa catactttct tacctatgaa
     8221 aaaaatagaa aagctgtaaa aagtctttta aaaggctttt tgcagcttga tttttaaaaa
     8281 aatgtttatg aataaaaaaa gttgttcccg gatttcccag aaaaggtggt agaaagtggg
     8341 ggattgtggt agactaattt tagttagtgg aggaatgggg ggctttcaat gttgatgggt
     8401 gaattccaac ataatattga tgcaaaagga cgtttaatcg tgccctcaaa acttcgtgaa
     8461 gaacttggcg aaaagtttgt tctgacacgc ggattggatg gctgtctttt cggctatccg
     8521 atgagcgaat gggaaaatct ggaagctaaa ctgaacgaaa tgccgttggc taaaaaagac
     8581 gcacgtactt ttgtgcgatt cttctattcg gctgccactg aatgcgaact tgataaacaa
     8641 ggacgaatca atatacccag cacgttaaga aattatgcag ctttgacaaa aggttgcgta
     8701 gtcatcggtg tatctaaccg aattgaaatt tgggatgaga cacgatggca agatttttct
     8761 gcagcagcag aagagaactt tgacgaaatt gctgaaagta tgattgattt cggactataa
     8821 gaataggaag aggctgacta atgactgaaa catttcaaca ctatacagtc atgttgaaag
     8881 aaacggtaga tggattgaac atcaaaccgg atggcgttta tgtagactgt acactcggcg
     8941 gtgccggaca tagtgaatat ttattaagcc aactaagttc taaagggcat ctgtatgcgt
     9001 ttgaccaaga tcaaaaagca atcgatcacg ccaagctgcg tttagcttct tatgtcgaac
     9061 aaggtatggt cacatttatc aaagcgaact tcagagaatt agaagaacgc ttgagtgagc
     9121 atgtagataa ggtagacggc attttgtatg atctaggggt ttcctctcct cagctagatg
     9181 aagcagaacg tggatttagt tatcatcaag atgcaccgtt ggatatgcgc atggatcagt
     9241 cagctccttt atccgcttat cacgtaatta atgaatattc ctaccatgaa ttagtaaaga
     9301 ttttcttccg ctatggcgag gagaaatttt ccaaacaaat tgctcgtgaa atcgaacgag
     9361 tacgaaaaac aaaaccaatc caaacaactg gtgagcttgt cgaaatcatt aaacaagtca
     9421 tcccagctcc cgcaagaaga aaaggcgggc atcctgcaaa aagaattttt caagctgtgc
     9481 ggattgcagt aaatgacgaa ctcggtgccg aagaggcctc tttagaacag gcaatccggt
     9541 tattgaaagt caatggaaga atcagcgtaa tcacattcca ttcattggaa gacagaattg
     9601 tcaaaagtat gtttaaagaa tatagtacgg tacaagatct accaccggga cttcctgtca
     9661 tacctgacga attccaacca gaactgaaag tcattactcg taaaccaatc cttccttcag
     9721 aggaagagct aacagaaaac aatcgatcac gcagtgcaaa gttacgtatt gcagaaaaaa
     9781 taagaatgaa agaggagtag aatgatggca gaattaaaga aaatgcaaga ttacgaatat
     9841 gatttacctg aattggtgga tcagccggaa caacaaatgc ctgattcttc ttcgaatcaa
     9901 gaaatcattc ttccacaatc accggcaaaa cgattaaagc agatttctgc tttggaaaaa
     9961 attgctgtgg caagcgtggc gattgctatt gttgctttat gtgttttaac agttatgctg
    10021 cgcacaaata tcagcggagc tgaaaaagaa atcacgtcaa tacaaacaga aatcaatgat
    10081 aaaaagcaag aaaaaacgag tttagaacaa gaaaaaaacg aactttctcg tactgaacgg
    10141 attaaacaaa tcgcagaaga aaaaggattg tctattaatg acgacaattt aaggaaagtg
    10201 aagtaaatga gtcttaaaaa taaaattcgg cggtttatgg aaaagaaaaa tctaaatccg
    10261 atgaacaatc gcaaaaaagt aggtatcatt ttatttgcta cgagtattgg aatgttcttt
    10321 ttatttgccg tccgtttctc gtatatcgtt attggaggtc atgttgctgg tacctcatta
    10381 gctgaaaaaa caaaacagtt gtaccaagga agtgaagtag taaaagcaaa aagaggaacg
    10441 atttatgatc gtaacggtgt ggcgctcgca gaagatgcct cctcttattc aattaaagcg
    10501 atactctcaa aaacatatac ctctggggat aaaaaactgt atgttgaaga aaagaatttt
    10561 gataaaatag cagaaatcct gcataagaat ctttctatag acaaaaaaga tgcactgaat
    10621 attttagagg acggcgcaaa aaaagagctg tatcaagttg aatttggtag ctatgggaaa
    10681 aatatctccc aagaaaccaa gcaaaacatc gaagcggaca tgaaaaaaga aggtgtcgct
    10741 ggtctttatt ttgtcgacca tcaggctcgg atgtatccaa atggcgtctt ttcttctcat
    10801 tttattggat acgcagttcc agacaaagat gagaatggct tagttggtaa actaggtttg
    10861 gagtctgctt acaatgatat cttgagcggt aaagatggta agattattta tcaaaaagat
    10921 aattttcaaa atcctctgcc aggtacagtg gcagaagaag aaaaagctgt cgacggacaa
    10981 gatatctaca cgactttaga cagcagacta caaagttatt tagaaacact gatggatcaa
    11041 gtaaatgaag aatatcagcc agaagaattg acagcagtcc tgatgaaagc aaaaaccgga
    11101 gaaatcttgg caatgggaca acgacctact ttcaatcctg aaacaatgga aggattgact
    11161 ggaaaagatg ctatctggcg taatttcttg gtacaagata gttatgaacc aggatcaacc
    11221 atgaaagttt tcactactgc agctgctatc gaagaaggag aattcaacga gaatgaaacc
    11281 ttccaatctg gaaaaatcca agtagaagat gctacgatca atgaccatga ctttggtgaa
    11341 aaaggtgtgc tgaccatgcg tcaagctctt tcatggtcaa gtaacgtcgg gatggtcata
    11401 ctggaacagc gattaggcgg acgatggtat aactatttac aaaaactggg atttggacaa
    11461 agtactcatt ctggattaga tgatgaagta aacggtgctt tacctacatt aaatattgtc
    11521 gaccgagcaa tgagtgctta tggacaagcg gtaggtgtga cgaatttcca aatgatgaaa
    11581 gctttcactt ctattgcgaa taacggtaca atgattcaac ctcgatatat cagcaaagtg
    11641 gtggatcccc aaactggaga agagcgaaca actcaaacag aagttctagg gcaacctttt
    11701 tcaaaagaaa caacagaaaa agtccgtgaa tatatgagag atgttgtcga aagcgaaaac
    11761 tacggtagtg cttatggtgt atatagtgta cctggataca atgtatcggc aaaaaccgga
    11821 acagcccaaa tcgcttctga tacaggggga tatcaaacag gagatacagc ctacctgtat
    11881 tcgattgtgg agatggttcc ttctgaagat ccagactatg tcttgtatct aacaatgaaa
    11941 caccctaaga catatgatcg aatggcattg gctaaaatcg caaatccatt gatgaaacgg
    12001 gcaatggatt ttaaagaaac tgaagaagat tcagatacag aaacaaaaac agaaaaagta
    12061 tccgtagccg attatcgtaa tcttgaagcg gacgttgcag cagctgatgc tcaaaaaagt
    12121 ggactgcaac ctgtagtgat cggtaatggt aaaaaagtac aaaaacaatc tactgccaac
    12181 ggtgatcagt tgatctctgg agaaaaactg atcctatata ctggtggtga taagctgatg
    12241 cctgacgtaa caggatggtc aaaagcagat atcatgaagc tggggaaaat cctcggtatt
    12301 gaagttagtt ttgatggaga tggttattgt gtcaaacaag aacttgctcc atatgaaaaa
    12361 atcactgaag acaaacttag ttttacttta gaagaataag aagagaaata ggagagaaaa
    12421 gagcatggaa tggacacaag cattgattcc cattgtcagc agttgtgcaa tgacaattgc
    12481 cgcaatgccg ctatttattg gttattttca aatgaaaaag caaggtcagg cgattcgtga
    12541 ggagggacca aagtggcaca atagcaaagc cggaactcct acaatgggcg gcttagtgtt
    12601 tctgatcggc tctattttga caggaatttg ggttggagct tggcaaaaac agctaactcc
    12661 tactcttttc atcttgcttt ttgtattagc gttatacgga gtaatcggct ttcttgatga
    12721 ttttataaaa atattcaaga aaagaaacat gggactgaac tcgaagcaga aattgattgg
    12781 acagattcta gggggaatca tcttttatct tgtttaccgt tctgaaggtt atccaggtgt
    12841 attgaatttc ttcggactag atctaccttt aggaatcatt tatggtattt tcgctctctt
    12901 ctggcttgtt ggattttcaa atgcagtaaa tctgacagat ggcatcgatg gattagttgc
    12961 tggcttagga agtatctctt ttgcagctta cggtctaatt gcttggcatc agcatcagta
    13021 tgatgtatta atcatttgct taagtgtttt aggaggttta ctgggtttct tcgtctacaa
    13081 cagaaaacct gccaagattt ttatgggaga tgtcggttca ttagcgttgg gcggactttt
    13141 agcggcaatt tctataatgc taaaccaaga atggacattg cttttgattg gtttgatcta
    13201 tgttatggaa acagcaagtg tgatgcttca agttacttcc tttaagctta caggaaaacg
    13261 aatttttaaa atgtctccaa ttcatcatca ctttgaaatg tgcggatggt cggagtggaa
    13321 gattgataca gttttttggt tgatcagcat tgtgacttcg ttgatcacat tatggatcgt
    13381 ttggtaggag atgacgggaa atgaaaaaaa tatcaacata tgaaaataag aaagtgcttg
    13441 tcttaggact cgcaaaaagc ggagtaagtg cggccaaatt attacatgaa ttgggcgcac
    13501 ttgtcaccgt taatgatgga aaaccttttg acgaaaatcc ggaagcacaa gagctgcttt
    13561 ctttagggat aaaagtaata acaggtagtc atccaatcga attgttggat gaagaatttt
    13621 cattgatggt caaaaatcca gggattccct acagtcatcc tttagtagca aaagcccaag
    13681 aaatgggtat tccggtaatt acggaagttg agctagctta cgaagtagcg gaatgcccaa
    13741 ttattgggat tacaggaacg aacggaaaaa caacgacaac aacgatgaca ggactactac
    13801 tgaatgcagg agcagatcaa ggaattgcac gtttagcggg taatattggg tatccagcaa
    13861 gtggtgtagc gcaagaagca aaatcagaag ataaaattgt gatggaattg tctagcttcc
    13921 aactcatggg aatcacagat ttccgccctc atatagcggt gattaccaat atctatgaag
    13981 cacatattga ttatcatggt acaagaaaag aatatgtcaa agctaaatgg aaccttcaaa
    14041 aaaacatgac agaaaaagat tatctgatcc ttaactggaa tcagtcagaa ctacaagaat
    14101 tagcgcaaag aacaaaagca cgcgtactgc ctttctcgac aaaagaagtg ttggaagacg
    14161 gtgtgtatgc tgacgattac tcaatatact ataaaaaaga aaaaataatg gaaatatctg
    14221 aacttggcgt accaggtaag cataatgtag aaaatgcact agctgctatt tctgtagcaa
    14281 aattgtatgg tatatcaaat gaagctatac gagaaacgtt gcattttttc catggcgtac
    14341 cacatcgtac gcaatatgta ggagaaatcc aaggcagaaa attttataat gattcaaaag
    14401 caaccaatat tttagcgact aaaatggctc taagtggatt tgaaacgtct aaggtcgtgt
    14461 tattggctgg cggattagac agaggaaata catttgatga attaattcct tcattaaaag
    14521 gaatcaaagc aatggtcgta tttggccaaa caaaagaaaa gttgatggat gctggaaaaa
    14581 aagcaggcat cgagacaata gtaacagccg attctgtaga acaagcagtt cctttagcat
    14641 tagaaaacag tacagatggt gatgtagttt tattatcacc agcgaatgcc agctgggatc
    14701 aatacccaaa ttttgaaact agaggaaaca gattcatgga agctgtgaac cgattaaaat
    14761 agaaaaagta gaagcgagcg ataaaaaatg aaaattttgg ttacaggcgg aggaacaggg
    14821 ggacatattt atccagctct tgcctttgtc aattatgtaa agactcaaga accgaatgct
    14881 gaattcatgt atgtgggagc aaaacgaggt ctagaaaaca aaatcgtccc tgatacagga
    14941 atgcctttcc ataccttgga gattcaagga tttaaacgaa aaatttccat gcataacatc
    15001 aagacggtcc gattgtttct taagagtatc cgtcaagcaa aaaaaatcct cagtgatttt
    15061 caaccagata tcgttatcgg tactggagga tatgtttccg gagcggtagt gtacgctgca
    15121 gccaaaatgg gtattccaac agttatccat gaacaaaaca gtgtaccagg tgttaccaat
    15181 aaatttttaa gccgatatgt ggatcggatc gctctttcat ttaaagatgc ggcttccttt
    15241 tttcctgaaa acaaagcagt tcttgtaggt aatcctcgag cacaagaagt agcagatact
    15301 aaaaaatcag atattttgaa aacatttgat ttggatccag aaaagaaaac agtcttgatt
    15361 tttggcggta gtcaaggagc acttaaaatc aatcaagcag tatcagtttt tttaagtgaa
    15421 tatattcctt cttcttacca agttttgtat gcttcaggag atagatatta tgaagagatc
    15481 ctaaataaaa taaatcctga aatacaaaat attagtgtcc agccttatat caaaaatatg
    15541 gcagaggtca tggcttccag tgatctgctg gtggggcgtg ctggtgcaac ttcgatcgca
    15601 gaattcacag ctctggggtt accggcagtg ttggttccca gcccatatgt tacgaatgat
    15661 catcagacta aaaatgcaat gagtcttgtt cacgcaggag cagcaaaaat gattgcagat
    15721 aatgaactaa caggtgaatc actaagtcag acagtcaacg aaatcatggg tgatgaagaa
    15781 ttgcaaaagc aaatgtgccg agcttctaaa gaacaaggaa tccctgatgc ttcaaaacgt
    15841 ctttatgact tagtaaaaca aataatcaaa aaataaaggc ggtggtctta gtgagtaaga
    15901 aaaaagaacc aaaaaaattg tctcatccgt cttctgatga aaacaatttg actccttggc
    15961 aaaaggctaa ccgaaaatat ctggcagaac aagagatgaa aaaccaagag ccaaaaaaag
    16021 aagacataac attagataca cccctttcag attctgaaga agaaaaaacg gttcaagagg
    16081 aacagcaaga agaaagccag tccgagaaaa aaatcgaatt ttttgaagag atcaagcaaa
    16141 agaaaaacgg tggaccatat aacggttctt ttttgaaccg gctgcccaat ttaaaaagtc
    16201 aacggcacaa agtactattt cgtcggttag ctttgatcat cactgtattg ggtataccgc
    16261 tgattttttt gatctattat gtttctcctt atagcaaatt acaagcagtt gcagtatcag
    16321 gcaataagac agtcaacagc caagaaatca tctctgatac aaaattgtca ttaggagaaa
    16381 atgtttgggg acagtatttt catcgatcta cttatatcga tcgccttaaa aaagcacagc
    16441 caagaatcga aacagcaaat atacatttca aagggatgaa tgaattcgat ttggatgtaa
    16501 cagaatataa agagattgct ttgatcgcaa aaaacaatca atactaccca gtgattgaaa
    16561 atggaacagt attagatgaa aaagtagcaa atcctacaaa aaatctccct atcttggaag
    16621 agttcaaaga ctctgcaaaa atcaaagaat taacaaagca atacaatcag ttatcatcag
    16681 aattacaaaa agcaatttct gaaatcaagt acacgccaag agcaagcaat aaaaatctga
    16741 tccaattgaa tatgaatgac ggcaatcaag ttattgtaaa tatcaataat cttgcgaacc
    16801 aaatgaaata ttattcgcaa gtagccaaag atatggatga aaaaggtgtg atcgacatgg
    16861 aagttggtat cttctcgcat ccttacggta gcactcaaga aacggaagaa tcgggccaat
    16921 cggaagaaac aactgaaagt gaagaaaatg cggatcaaac aaacgaatca agtgaagagg
    16981 cagaaacgca aaaagcacaa acacctggat cgtctagcac aaacggatca tctgattcaa
    17041 atgattcaag tgaaacaagt caaatagaag ataatccaag tacaactccg tcaagtttaa
    17101 atgaaaattg ataaaacaac tttaaaaagt aagaatgtac tttctaaaaa ccagtaattt
    17161 atggtagaat tggaagcagt gaatatatct attaataagt atattttaaa acggacgcta
    17221 gttgttcgct tgtatatgga atagtaagta ggagggaatc ccattcatgg caaaaacagg
    17281 aatgtatgta ggccttgata ttggaacgac atctgttaaa gttgtcgtgg ctgaatatat
    17341 cgacagccaa atgaatatta ttggagtagg aaacgcaaaa tcagaaggga ttaaccgagg
    17401 cattatcgtt gatatcgata aaacggtcca agcgatacaa cgagcagtac gacaagcaga
    17461 agaaaaagca ggtatccaga tcaaaggcgt aagtgtcggt ttgccagcaa acatgcttga
    17521 agtagaaaat tgccagggaa tgattgcagt taacggagat tcaaaggaaa tcaccgatga
    17581 agatgtcaga aatgtggctt cagctgccct tgttcgttca atacctcctg aacgtcaaat
    17641 cgtgtcaata ttgccacaag attttactgt cgatgggttc gagggaatca aagatcctcg
    17701 tggaatgatc ggcgtaagat tagaaatgta cggtctgttg tttacaggac ctaaaacgat
    17761 catccataac atacgtaaat gcgtggaaaa tgccggatta atcgtcaacg aaatggtgat
    17821 tactccttta gcgctgactg aatcaattct ttctgacgga gaaaaagatt ttggtacgat
    17881 cgtcattgat atgggtggcg gccaaacaac aacggctgtt atgcatgata agcaattgaa
    17941 gtttaccaat cttgaccaag aaggtggcga atttgtcact aaagatatct ctattgtatt
    18001 aaatacgtca tttaacaatg cagaagcgtt gaaaataaac tacggggatg catacccaga
    18061 acggacatca ccagatgaag aatttccagt agatgtgatt ggtcagtcag aaccagtgaa
    18121 agtcgatgaa cgctacttgt cagaaattat ttcagctcga atggaacaga ttttcaacaa
    18181 agcaaaagat gcattggacc aaatcgaagc attagagttg ccaggtggcg tcgtattgac
    18241 aggcggagct gctagtcttc ctggtgttgt ggacttggca caagaaatct tcggcgtaaa
    18301 tgtaaaattg tatgttccaa atcacatggg gctgagaaac ccggtattta caaatgtaat
    18361 aagtatcgtg gattattcag caaacttgag tgaagtgtat caattggcaa aaattgctgt
    18421 tacgggtgaa acctcagcag ctcgtcaaat ggtagttgaa caagaaacaa caaacacata
    18481 tgagagctat gaagcacctg aagaaacaat ctacgatgag ccggaaccaa aagagtccgg
    18541 agaaaatgta aaaaataaaa tcaagggttt ctttacaaat atttttgatt aattcatcct
    18601 gggaggaata agatatggaa ttctcaattg ataataatat caatgacggc gcagtaatca
    18661 aagtcatcgg agtcggtggt ggcggtggaa atgctgtaaa ccgaatgatt gaagaaaacg
    18721 ttaaaggtgt tgaattcatt acagccaaca cagacgtgca agcattaaaa aattcaaaag
    18781 ctgaaacagt tatccaatta ggccccaaat atactcgtgg tttaggtgct ggttctcagc
    18841 cagaagttgg acaaaaagcg gcagaagaaa gtgaacaatc cttacgcgaa gctttagacg
    18901 gtgcagatat gattttcatc actgcaggaa tgggtggagg aaccggtaca ggtgctgcac
    18961 caatcgttgc aggtatcgcg aaagaattgg gcgcattgac tgttggtgtc gtgacacgtc
    19021 cattcacttt tgaaggaccg aaacgtggcc gttttgctgc agaaggtatc gctcgtttga
    19081 aagaaaatgt ggatacatta ttaattatct caaataaccg tttattagaa gttgtagata
    19141 aaaagacacc gatgcttgaa gctttccgtg aagcagataa tgttttacga caaggtgtac
    19201 aaggtatctc tgatttgatc acagcgccag gttacgttaa ccttgacttt gctgacgtga
    19261 aaacagtgat ggaaaaccaa ggtacagcat tgatggggat cggtgtggct agtggagaag
    19321 atcgcgtagt agaagcaacg aaaaaagcaa tctcctctcc attgcttgaa acatctattg
    19381 atggagctga acaagtactt cttaacatca caggcggttt ggatatgaca ttgttcgaag
    19441 cacaagatgc ttcagatatc gtagcaaatg cagcaactgg cgatgtaaat atcattctag
    19501 gtacatctat caatgaagaa atgggcgatg aaatccgtgt gacggtcatc gcaacaggta
    19561 tcgatgaatc aaaaaaggaa cgcaagtcta gccgaccagc tagacaagca caaatgcaat
    19621 cacctgctca aaagactgta ttagatatgg atcaagctaa accaacttct tcagaagaag
    19681 aaaacagttt tggcgattgg gatatcagac gtgaacaaaa tgttcgtcct agagttgatg
    19741 attccaattt tgacaatatc gaaaaaaaag aatttgatac attcaaccgc gaagaaacaa
    19801 aatctcatgg cgatgatgaa ttaaatacgc caccgttttt ccgtcgtaaa aaataaagga
    19861 gaggaagagc acatgattgc tgacaacttg cagaaaatca agcaagaaat tcaggattcg
    19921 tgtgctcttg ttcatcgtga tccgaaagaa gttacattag tagctgtaac aaaatctgtt
    19981 gaatccgctc aagctgaaga attggttaga gctggcgtta tgaattgtgc agaaaaccga
    20041 gtagataaat tattagaaaa gaaacaagat ttatttgatt ttcctgaaat aaagtggcat
    20101 ttaattggta atttacagcg cagaaaggta aaattgattg taaatgaaat tgattacttt
    20161 catgcgttgg acagtttaag attagcagaa gaaatccaga agcggttaga gaaagaatta
    20221 tcctgtttta ttgaagtaaa tgtttctggt gaggaatcaa aacatggaat ttcaccagat
    20281 gagcttttac cgtttgtgga atctttagct gcctgcacaa aaatcaaggt tgttgggttg
    20341 atgactatgg cgcctaaaga agcttctgat gagcaaatcc ataaagtgtt tggtacgctg
    20401 aaagaattgc gtgatcttgt tcaagcaaaa ggttatgccc atgcaccatg tagtgaatta
    20461 agtatgggga tgagtcaaga ctttcaaatt gcgattgaag aaggtgcaac atttatccgt
    20521 gtaggaacag cgctattcaa agacgaataa aggaggaaca aaaatgccgc ttttaaacag
    20581 aggagcaatt gctaattttt tcggattagc tgatgaggaa gagtacgaat accaagatta
    20641 tcctgaaaca acaaaaaagg ctgtacagcc aactaaaaaa aatacaacac agaatcaatc
    20701 aactcattct gtatctgctg cgaatactca tgaaaaacaa aaaacggcga cttcacgtcc
    20761 tgtaacgcat gaaagtattc agccggctga aaagaaaaaa gtaattgaac tacatcaatc
    20821 acctacaaaa actgtagcga aagaaagaac agcttcaaga acctctacac aacctaaagc
    20881 aaatcttgct ggaaaaatca ctgtaatgga accaagagct tattctgaag ctatggcgat
    20941 tgctaaacga atcattgctg gagaagcaac cttagtgaat tttcatttag tagaagaaaa
    21001 acaagcgaga agaatcgttg attttctaac aggaacagtt tatgcactag atggagatat
    21061 caaacgtgtt ggagatgaga tttttctttg tacaccgact gggatggaga ttgacagttc
    21121 aaccgctcaa tcttttgcag atgcgaattt gtttgatctg taagaggagg aactacgatt
    21181 tatactattg cactattact tcatttactt caacgggcag cctatttcta tagcattatc
    21241 ttgatggtat atgcattgct ctcttggttt ccaggcgggt atcaatcagg gctaggacga
    21301 ttattagcta agatctgcga accgtatatc agtttgttcg atcgtctgcc tttgaggatt
    21361 ggacccttgg atttcacaat tttaggggca atcctgatct tgaacttagc cgtacaaggt
    21421 ttagcacaga tcgttatatg gataactgtc catttagttt attgattgaa gcaacaaaat
    21481 ctttattctc agaatagata ggaggggaaa attttggatg caaatgtata ccagcacttt
    21541 cgggcagatg aacgtacatt tatcgattcg gtaggcgatt ggattgaaca ggttcaagga
    21601 caatatgcac cctatctgac cgaatttctt gacccaagac aatcctatat tttagaaaca
    21661 ctgattcgac aaagcagtga tttgcgtttc atgttttatg gcggatatga acaggcagaa
    21721 agaaaaagat gtttgatttt ccccgattat tacgaacctg tagaggaaga ttttgaaaca
    21781 gcattgtatg aaatccatta tcctagcaaa tttgccagcc taagtcatgg gaaaatcctt
    21841 ggaactttag tgggaacggg gatcaaacgc tcttactttg gcgatatcat ctcggacggt
    21901 gatcgctggc aagtatttat tgcaaatgag gtggatcatt ttgttgctgc acaagtaaca
    21961 aagattggaa aggttgctgt acgcttggaa ccaatcaaat acacagaaat gattatgccg
    22021 aaagatagct ggacacaaga aagaggaaca gttagttcca tgcgactaga cagtgtgatc
    22081 tctgagattt ataatatttc cagacagcgg tccaagcagc tgatcgaagc tggtaaagta
    22141 aaagtgaact ggacagaaaa tacaagacct gattttgtac ttgaactatt agatattatt
    22201 tcagtcagag gttatggcag agtccagatc caagaacttg aggggaagac gaaaaaagaa
    22261 aagtacaggg tactgttcgg tgtattacgt aaataaaata aaaaggtggt ttactcatgg
    22321 ctttaactcc attagatatc caaaataaga cattcccgac aaaaatgcgg ggatataatc
    22381 aagacgaagt agatgatttc ttagatttag ttgttcgtga ctatgaggaa ttgactcaaa
    22441 gaaatcgaga attagaaaaa gcagtcaaac attctgaaga aaaacttgag tacttcaatg
    22501 aattaaaaga tgctttgaac caatcaatca tcgtagctca agatacggct gataaagtaa
    22561 aaacaagtgc aagcaaggaa tccgaagtga tcgttacttc tgcacaaaac aaagcagatg
    22621 agttagttgc gaacgctgaa aaacgtgcac atcaattaac aactgatgca gaagaaaaag
    22681 caagaaaaat cttgacagat gcaacagaaa aagcaagaca gcttgcgaca gaaactgaag
    22741 atcttaagaa aaaaacacga gtattccatc aacgtatcag cttgatgctt gagtctcaat
    22801 tagaacaagt gaaaagtccg gaatgggatg aaattttaca accattctcc agctacgtga
    22861 ctgacagcca tgaagtgatc aaagaagttt tagcaaaaca gcttgacaat gaaaatgata
    22921 ctgatgtaaa ctcagacaca ggagtcatag aagcacctat gacggcattt gatacacagg
    22981 ctattgatat ctcgatcgtg cctggtgaaa atgaaaacga gtagaacaga aaagcacttt
    23041 gttattctta aagcgagccg atgagagtgg aagttcggta agacactgca tggtgaaaga
    23101 tcctctactt gagacctgct tttccgaaat tagagtaaga agcagcggct gacctcgtta
    23161 ttggtcacag agagtttgca ctagatagat gcaactaaat ttgggtggta ccacggcatg
    23221 ttcgtccctt gcggctgaac atgctttttt gtgtccggaa aacaaataaa ggagtgtatg
    23281 gttgaatgaa aatgaaagaa accttgcaat taggaaaaac aaaattccct atgcgtggta
    23341 atttgccaaa ccgagaaggc gagtggcaaa aagaatggga agaaaatcga atctaccaaa
    23401 aaagacaaga gttgaacgaa ggaaaaccta gctttgttct tcatgatgga cctccatatg
    23461 ccaatggtaa tatccattta ggacattcat tgaataaaat cagtaaagac attatcattc
    23521 gttcaaaatc aatgtctggc tatcgtgcac catatgttcc aggatgggat acacatggat
    23581 tgccgattga acaagtattg gctaataaag gcgtgaaacg taaagaaatg tccatggcag
    23641 aatacttgga aaaatgtcgt gaatatgcac tttcccaagt agataagcaa cgagcagatt
    23701 tcaaacgcct aggagttgca ggagattggg aacatcctta tgttacatta gatccttctt
    23761 atgaagctgc acaagtacgc gtatttggta aaatggctga aaaaagctat atttacaaag
    23821 gattgaaacc aatttactgg tctccgtcaa gtgagtcttc tttagcggaa gctgaaattg
    23881 aatataaaga tgtaaaatct ccatctattt ttgtagcttt caatgtcaaa gacggcaaag
    23941 atattcttga tgaagataca gcttttgtca tttggacgac aacaccatgg acattgccag
    24001 caaaccaagg tattgctgta aatccaaaat ttacgtatgt attggtggaa gccgatggcc
    24061 gtaagtttgt cgtagcgaaa gatttgctag aaacagtcca atcagaaatc ggttgggaaa
    24121 atgtaacagt tctaaaagaa tttgctggac aagaaatgga atatatgact gcaagtcatc
    24181 cattctatga acgtgaatca cttgtgattt taggcgatca tgttacttta gacgctggta
    24241 ctggattagt ccatactgct ccaggacatg gggaagatga ctatatcgct ggtagcaaat
    24301 ataaactatc tgtagtgtca cctgtagact caaaaggatt gtttacagaa gaagcacctg
    24361 gatttgaagg tatcttttat gacaaagcaa atcctatgat cactgatcta ttaaaagaaa
    24421 aaggagcttt gctgaagcta gatttcttta ctcatagtta tcctcacgat tggcggacga
    24481 aaaagcctgt gatctatcgg gcaactccac aatggtttgc atctatcgat aaattccgtc
    24541 agaatatttt ggatgaagta gaaaaagtag agtgggttat cccatggggt aaaacacgtt
    24601 tgtataatat gatccgtgac cgcggcgatt gggtaatttc ccgtcagcgt gcatggggag
    24661 taccacttcc gattttctat gctgaaaatg gagaagcaat catcacacca gaaacaatcg
    24721 aacatgtggc aaaattattt gctgaacatg gatcaattat ctggtttgaa aaagaagcga
    24781 aagaacttct tcctgaagga tttacccacc caggttctcc taatggcgaa tttacaaaag
    24841 aaaaagatat catggatgtc tggtttgatt ctggttcttc tcatgaagct gttttacgtc
    24901 agcgaccaga attatcattc ccagcagata tgtatttaga aggatcagac caatatcgtg
    24961 gctggttcaa ctcaagtatc acgactagtg tggctatcaa tggtgtagca ccatacaaat
    25021 cagttatttc tcaaggattt gctttagatg gtgaaggccg taagatgagt aaatctttag
    25081 gaaacatcat tgcaccagaa aaagtgatca aacaatttgg tgcagatatc cttcgcttat
    25141 gggtatctag tgtagattat gaagcagatg ttcgtgtttc tatggatatc ttaagtcaag
    25201 tggcagaagt atatcgtaaa atcagaaata cgatgcgttt cttattagcg aatacagaag
    25261 atttcgatcc taaaaaagat actgtttcgt acaatgattt acgttctgta gacaaatata
    25321 tgaccgttcg attgaaccaa gtaatcaaag aaatccgaga agaagggtac gacaaatata
    25381 acttcatgca tatttaccgt acagttatga acttcttgac ggttgatcta tcgtcattct
    25441 acctggattt tgctaaagat gtagtttata ttgaagcaga agatgattat caacgtcgtt
    25501 gcatgcaaac tgttttctat caaacagctg tagctttgac aaaattattg actcctatca
    25561 ttccgcatac agcagaagaa atctggtcat tcctgaaaga agaagaggag tacgttcagt
    25621 taagcgagtt tcctggttat gaagagtttg caaaccaaga agaattgatg gatacctgga
    25681 ctgctttcat ggacttccgt gataatgtct taaaagcatt ggaagaagca agaaacagca
    25741 aattgattgg taaatctttg gaagcaaaac taactgttta tccaaaacaa caagttcgtg
    25801 aaatgttaaa agctttggat gcagatattg cacaattatt gatcgtttca ccagactact
    25861 ttgaagttat ggcagcagat gaacaagtgc cagataacgc tatggtattt gatgatgttg
    25921 ctattacagt ggaaaaagca gacggggaaa cttgcgatcg ctgtcgccaa gttcgtaaag
    25981 atgtaggagt agatgaaaaa cttcctcatc tatgcggtcg ttgtgcaaaa atcgttgaag
    26041 acttctatcc tgaagctgta gctgaaggat tcgaagaaaa gtaaaaggga aaagggggtg
    26101 tgacatgttc tttgtcacac ccccttttgt tatcaatcgt ttaattttcc acgttcacga
    26161 taccagttat ctggttgcca gatccattcg aatccgtctt tttcaagtag ttcgaaagct
    26221 gctttaggtc ccattgatcc agctttgtag ttgggaaaat ccggttgtgt ttggtcccaa
    26281 gcttttcgaa tgacatccac gatatgccaa gattgagcga cttcttccca atgagagaag
    26341 ttcgttccat caccattcaa cgcatctaat agtaattttt cgtaagcttc tgggctattt
    26401 tcaaccattt ctgcactatt tctaaattcc aatttgattg gttccgtttc aaatccttgc
    26461 cctacttctt tcccattcaa ggataaagaa aatccttcag ttggctgaat atagatcgtt
    26521 agaacattag gtggaagtgt cgtatcgtca catggttcat cgacagatgt tttgaataca
    26581 ttaacaggaa cttgtttgaa tacgatgttg atccgagtcc ctttttcagt caaacgtttg
    26641 cctgtacgta catagaaagg aacacctgac caacggaagt tatcgatagt gaatttgcct
    26701 gcgacgaatg tttcagtctc tgattgagca tccactttgt cttcttcgcg atagcccttg
    26761 aattgtacac catccaattc accttgagca tattgcccgc gaacgaagtt ttctaacact
    26821 tcttcttcgt tataaatgcg gatagctttc aatgctttga tcttttctgt acggatttct
    26881 ttttcggaaa atgctactgg cggttccatt gcaagtaatg ctacaacctg taaaatatga
    26941 ttttgcacca tgtctttaag tgcgccacta tgatcgtagt agcccccgcg atcttctaca
    27001 ccgaggtttt cgccaaaagt aatttggata ttgtcgatat agcgattgtt ccattgagcc
    27061 tcaaaaatat tattggcaaa acggatagct gagatgtttt ggatcatttc ttttcctaaa
    27121 taatgatcaa tcctgaaaat atcttgttct gggaatactt ctctgatttg ctggttcaat
    27181 tcaaaagcag actggtaatc agaaccgaac ggtttttcta tgatcaaacg attgaagcca
    27241 ttttttgtca taatattttg tgatttcaaa tgagaaacga tcgttccaaa aaattgaggt
    27301 gccatagcca aataatagac acggttaccg tttaattgat agtgtccatc taatcggtca
    27361 gacaattctt ttaatgtctg ataatgcgtt gaatcattta catcatggga ttgatagtaa
    27421 aaatggcttg caaaggtagt tgcttcttca tctgaaggat tcaatgcacg gattgtttct
    27481 ttgacaactt cacggtagtg ctcatcgctc cagggacggc gagcggttcc gattacagca
    27541 aaatgctcgc ctaaatttcc ttttctgtaa aggcggaaca aagacgggta caatttacgc
    27601 tgggctaggt cacctgtacc gccgaatatc gtaaacaata catttttttc attagacatg
    27661 tggatacact cctttatatt ttatcgacaa aaatcgtgct ggccgcctta aagctgacgg
    27721 ggattttctt tctatcattt tggatagtga tcggtccttc ataagctgcg atgtcaacga
    27781 tctcatattc ttcgttgata ttcaggtcaa tagtgactaa gtaatccaac aattcttttt
    27841 catcaagcac tcgtgcgata cgcacttttg taccgacagg ataggactcc aatgtttgtc
    27901 ggcgttcttc atggacaact tcctcttttt taggaatcga tcctccatga ggacaatgtt
    27961 caggatgatt caaatagttt tctaaatgat ctgcaagatg ttccgaagtc acatgttcca
    28021 atacttcagc atcatcatgg acttcattcc agctatagtc taaatgttca actaaaaaaa
    28081 cttcccataa gcgatgtttg cgaatcagac tgctagcttt ttgcagtccg cttgcagtca
    28141 attgtacacc ttgataaggg gaatgttcta caagctcttc tttcacaagt ttagaaatca
    28201 tttcactgac agatgcagct gagacatcta gactagatac gatttgttta ttgttgactt
    28261 tcgttgtatc tccacctagt tctaaaatga ttttcaaata atcttctcga tttggtgtca
    28321 tattcgatca tcctttcatg gtaaaaaacc atcattagta agtgtaacaa aaaaaacgtt
    28381 aattgactat ttttctaaat aaaaaacaaa ggatttttag acgtttttac aacaaaaaac
    28441 gaaagatgat agagaagact cattctttcg tttataaagg atatatatat acattgggag
    28501 ggatctattt ttcagataac aacttatatg aagtgtctgc agattcgtca taaagagttt
    28561 gttcgggata agaaaagata agataaaaac catactgatc acgtacgact aaacgatatt
    28621 catctatttc taccacagat gtgccaagag actgaccgtt gatcaaaaac gtgtaatcag
    28681 atgtaatctg taattgttgt ttttgctggc tatcactaaa gaaccatgta ccttgatact
    28741 ttgagagtcc ttctgacttt tccgcttgtt ttttcttttt caagtaaata aaacttgaga
    28801 gaacggtaat gagtgaacca agaaaataga tcattttttt tatcaccaaa tcactccttt
    28861 atgagaaaat aatcgcatag acatattctt tcttctttat ttcattgtac cgaataaaag
    28921 ctaaaatttc tctaagactt tcgactggtt tttttactgt ttttctacaa attatgatag
    28981 gatacatagt aggaagaaag gtgacataat ttgaagtatc agcaagtgga aatagctttt
    29041 tttattgacc gcccgaatcg gtttatagca cattgtatga aaaaagacgg agaaataatt
    29101 attgcgcatg tcaaaaatac aggtagagga aaagaggtcc tgataccagg cgctgaagtg
    29161 gctattgtat ttgctcctgg acctaaacgg aaaaccgcct atgatctgat tgctgtaaaa
    29221 aagaagggtg actggtttaa tatcgatagt caattgccta atcggctggc aattgatggt
    29281 attttagacg gcacgatcct tttaccagga ttaactggtc catttacggt atacaagcgt
    29341 gaagtaactt atcttcactc caaatttgat atttatttag agacggtggt cggacaaaaa
    29401 gcatttgttg aagtcaaagg tatgacactt gaaaataaag cgattggtgc ttttcctgat
    29461 gcgcccacac tcagaggatt gaaacatata ggagaattaa cgtatgccgc acaagatggt
    29521 tatgccgctt atgtcttgtt tattgctcag ttcgaacatc ttcatctggc aacgatccat
    29581 gaagaaatgc aaccagcact agctgatatg gttcgacatg cgcagcaaag cggagttcaa
    29641 atcctcgcct ataattgtca agtatcggca gatgaggtaa ctgtcaagca ggccattcca
    29701 tttgatgtag atatcccttt tgaagatcct atacaataga aagaaggtaa aataatgatc
    29761 aacttaggta ttatcggaac aaattggatt actcatcaat ttgtccaagc agctttaagc
    29821 acaaaaaaat atgaattgac agcggtatat tctagacatt tggaaaaagc acaggaattt
    29881 ggttctcatt atgaagggga tatcgaattc gcaacagatt tgaagacttt tttcaatatt
    29941 actcatatgg acaccattta tattgcttct ccgaatagtc ttcactttag tcaagctaag
    30001 caagcaattt tggcaggaaa aaatgtgata gttgaaaagc cagctttttc gacgccaaaa
    30061 gaaatggatg aaatcatcca tttggcaaat gaacacaatg tgtatttttt tgaagcagca
    30121 agaaatatcc atgaatcagg ttttaaaaag attgccgatt acttaccatt aaaagacgag
    30181 ataatcggtg caaatttcag ctatatgaaa tattcttcgc gctatgacca agtattagat
    30241 ggagaagaac cgaatatctt ctcgactcat ttctcaggtg gtgcattgat ggatttaggt
    30301 gtttaccttg tatatgcagc tgttgcttgg tttggaatgc caaaagaagt ccattatttc
    30361 ccaagaaaga tttctactgg tgtagatggt cttggatggg ggatcttacg ttatgagaat
    30421 tttgatgtat cgatccagcc aggtaaaatc ggtgattctt atttaccgtc agagatttat
    30481 tttgactcag gaacgctagt gatgaatgga gtcaatgcaa ttgaacaagc acaatttcat
    30541 gatcgcgcgc ataaagaaat cattgatttg gcggtaacag ctgcagaaaa tccaatggca
    30601 gatgaagcag aggattttgc caatgtcatg gctcatccga aagatccagc ttggggattg
    30661 ttgtatgaag agtggatcga gctagctaga aatgtgaacc aagtgattta tgatttaaga
    30721 aaaaatggcg gcatcaaatt cgatgcagat aatgaggaag atttttaatg aacgaatttg
    30781 tacaagtatt tcccgaaaat tggcagaaaa aatatgagga taaagggttt atctcacctt
    30841 ctcttattca acgagcagtt tttcagccac taagtaataa acaaagtata gtagcggttt
    30901 ctcctacagg ttcaggtaaa acactggctt atctttggcc tctactacta aacgtggaac
    30961 caggtgaagc aagtgcgttg gtcatttttg cttcttctca agaactagct attcaagtag
    31021 cagatgttgc acgtgaatgg ggaaaagaca aagagttgaa agtccaatct ttggtgggcg
    31081 gagccaacgt caaacgtcag attgagggtt tgaaggaaaa accagaaatc ttgattggta
    31141 caccaggaag aatattggaa ttgatgaaag caaaaaaaat aaaagcacat caagtgaaaa
    31201 caatggtatt tgatgaagca gaccagctat ttgacgctgg gaacagccaa atcattgatc
    31261 aaattcttca tcaagctcca acagagtatc agctagcttt tttttcagcg acagcagatc
    31321 gttcccttga aagcattgaa aaaatcactg ggaaaacgtt tgagacaatt gacgtgacag
    31381 cagaggacga ttctcgaaaa ggactgagac attacttttt acgtgtaccg atacgaaaga
    31441 aagatgacta cttgagacgt ctcacgcata tagaagattt tcatggtctg attttcttta
    31501 atcaactgac tgaattaggc gtaatggaag aaaaattgct gtatagaggt gtaccggtag
    31561 gaagtctggc ctctgatcag aacaagctac tccgaaaagc agcgctagaa caattcaaaa
    31621 agaaaatggt cagagcactt ttgaccacag atgtggcggc tagaggttta gatattacag
    31681 gtcttcctta tgtagtgaat gcagaagttc cgttaagtga agaagcttat ttgcatcgtt
    31741 cgggaagaac tggtcgaatg ggtaatgaag gaatagtaat tacactcgta caagataata
    31801 atctaagaga tttaaaacgg cttttacgtc catcaaatat aacacttgaa gagatttttt
    31861 tatacggagg taagctgcaa actgagcagc caaccaaaga agatgcagct cctgcagtaa
    31921 ataaaaagta tgcgaaaact ttttctggaa aggagcagaa cccagaatat aacaaagaaa
    31981 aaataagaaa aaaagttaaa aataaatcaa aaaaagagag aaataaagga gcaaggcgca
    32041 aataagcgcc tttttatgct atgattatga aaacgggaac aaaattggtt gccagaggag
    32101 gggttatgtg aaagaacgtc aagaagagat cttgcagcta ttgacagaaa accaacaagg
    32161 attgacagca agtgatgtag ctgagcgctt aactatcgat cgaagcaatg caagccgata
    32221 tttaagtgaa ctatacaagg cccatcatat tgtaaaaacc gctggccgac cagtcgttta
    32281 ctctttgcca acggaaaagt caaaatctga tgaagtgcat gtcgactctt ctacgcaagt
    32341 gacttttgaa acactcgttg gagaaaatga ttctttaaaa gtcagcatac agcaggcaaa
    32401 agctgcgatt ctttatccac cgcgggggtt gcatacgatt atttttggag aaactggtac
    32461 agggaagtcg atgtttgctg aatgtatgta tcattttgca atcgattcag agatgctttc
    32521 tgcagatgcg ccatttgttt ctttcaattg tgcggattat gctcaaaatc cgcaactgct
    32581 ctttggacac atctttggga tcaaaaaagg ggcttacaca ggagcagctc aagatagtcc
    32641 cggtctaata gccaaagcag acggcggtat attgttttta gatgagatcc accgtttacc
    32701 gccggaaggg caggaaatgc tgtttacttt cattgataaa ggtatttacc gtccgcttgg
    32761 agaaagtgca caagtacatg aagcatctgt tcagatcatt ggggcgacga ctgaatcttc
    32821 agaaagtttc ttatctacgt tcaatcgacg gattcctatg gcaatcacct tgccgaatct
    32881 tgcttcacgt tcactggatg agcgttatga aatcatctct ctttttatca aacaagaagc
    32941 gaatcgtttg aatcaacgta tcgatatcga aaaagaagcg attcttgcct tcatgcttta
    33001 tgatgcggaa ggaaatatcg gtcaagtgaa acgagacttg aaactagtct gtgcaaaatc
    33061 ttttttacat taccgtactc atcatgagaa aaagctaatc attcgaaaag aagaactttc
    33121 ccttcaagta caaaaaggat tactgaaaat caaggaaata ccggaacgtc tcgaccgatt
    33181 catggacagc aaaagtcagt atttgacatt tgaaccagga tttgctgatg ttgtctggtc
    33241 acaagatcca gaacgaaaca tgcaggtcta taacgatatt gaggaaaaag tcacttctct
    33301 ttccgaaacc ggtgtggaga acattgatct agagaccctc atttcaaaag atgtggatgc
    33361 ttatttccag acatatgtca aagaattgac taaaagttcg attcccaaag atcttttgcc
    33421 agatgaaatc tggcggttaa ccaatcaatt gtatgatgtg gctgaaaaac ggttagaccg
    33481 tatttataat gaaaaagctc gttttgcttt tgctttgcat ctgcaaagta cattggatcg
    33541 tgtgagagaa ggtcatatga tcgtccaccc agatttgaat aacgtacgga aaaattttaa
    33601 gagggaattc caggtagcaa tcgatctttc aacgatcatt gaagaagaag aacaagttga
    33661 gattcctttt gatgaaatcg gctttattag tatgtttctt tctatcaatc taggagaaag
    33721 tgagcctgtt aaagaaaaca aagtagatgt tctcatattg atgcacggca gacagactgc
    33781 ttcaagcatg ttggagatgg cacaagaact attaggaaca aaagtaggga aagcttttaa
    33841 tatgccactg gttatggaag ttcaaacaat gtatgaagaa gtgttacaat atgtcaaaaa
    33901 ctatcaagag catttgacga atggcctact cttgttaaca gatatgggat cattaaacac
    33961 cttcagtgat ttgatttatg aagagactgg cattcggacg aaagctatca cgatgacaag
    34021 taccatgatt gtgatagaag cgatacggat ggcaagtgtt ggaagaagct tagaagatat
    34081 ttatcaaaat atccagcttt cctttgaatc gatcgtacgt gatcagttca aagcggaaaa
    34141 gtctgaaact ttacagcgta aaaaagccgt aatcgtgaca tgctttactg gtgaaggagt
    34201 agctgccaaa ttatatcagc gtattatgcc agtgattaat caaaataagg tagaactgat
    34261 ccagatgcag tttttagaac gtgaatcatt caaaaaacac atcgatgcct tattggaaga
    34321 atatgagatc aaagcaattg cgggaacagt tgatgttgat tatcaaaata tcccattttt
    34381 ttcagcctat gacgtttttg atgatgaaaa actaaatatt ttaaagcgta tagctgatga
    34441 tgagatcccg acagctaaaa ttgtcgattc acttgctgga tcattaagcg atgtcttttc
    34501 tattgatgaa ctgatccgtt cattgcaaaa gacagttcag cagatccaga ccgatttgtc
    34561 gattatcgtg gaacctggag tagatactgg aatcgttatc catttagcct tcatgttaga
    34621 cggattgatc aaacaagaaa gccatcggca atttgagaaa ctgtctattt tctcaaaaat
    34681 ttatcgtttg gaaatggata tcgttcgggc aaacttgatg gcactagaac gaaaatatca
    34741 ggtaaaaatt ccagaagatg atatcgcatt tcttacacaa atgtttcttg aaaataaaat
    34801 aaaaaaatga acacactaat catactgttt acacactaaa catctgtgtg cgcactataa
    34861 aaaagagcta aaaagcatga aaacgctatt tcagctcttt tttttattgg catgcttctt
    34921 gcgttatata aaagtgtaaa aaaataaaag gagtgttaaa aatggcaaaa aaaacaatca
    34981 tgttagtttg ttcagcaggg atgagtacga gtttattggt cacaaaaatg caaaaagcag
    35041 cagaagaaaa agggatggaa gccgatattt ttgcggtctc tgcctcagat gcagataata
    35101 atttagaatc taagaatgtc gatgtattat tattaggtcc tcaagtacgt ttcatgaaag
    35161 ctcaatttga acaaaaactt gcgcctaaag gtattccatt agatgtcatc aatatgcaag
    35221 actatggcat gatgaacgga gaaaaagttc ttgcacaagc agaaaatcta atgaaataaa
    35281 tcaagtatgc agttgggggt ataacaacgt ggaagatcaa caaaatctag aagccatcat
    35341 ggggttaatc atgtttggcg gtaatgcaaa gagcgatgca atggaagcga ttgcggctgc
    35401 taaaatcggt gattttgagt tagcggatag gaaaatcgct gatgcagaag aatcacttat
    35461 acaagcgcat catgcacaaa cagacatgtt gacacaagaa gcacaaggaa atcatatgca
    35521 agtgacatta ttgactgttc acagtcaaga tcacttaatg acatctattg cgtttactga
    35581 tttagcaaaa gaaatcatcg aactttatcg tcgcgtatac agcgaataaa tacaacttct
    35641 tttttgaacg gaagaagaaa aatttttgaa cggatttttt attttgttat aaggagaact
    35701 ctctttatat aaaaaactta taggaggaag taaaaaatga acggactatc aagttggatg
    35761 gagaaataca tcctgccggt tgccgcaaaa atcggagcgc aaaaacattt agttgctttg
    35821 cgtgatgctt ttatcggcac actgcccgca acaatggcgg gatcagtcgc tgtaatgatc
    35881 aatgcaatta ttcgtgattt accgcaacag tttaacagta cctatgctga cagtttagca
    35941 gcagacaaag gaatctttgc cgtattgaat gtcatcatcg gaatcaatgg ttttgtctgg
    36001 aacggtacac tagcaatcgc cggattgatc tttgcctttt cttgggggta taatcttgcc
    36061 cgagcttatg gagtaaatga tctagctggg gggatcgttg gtttagctac cttgattcaa
    36121 ggtatttcct ttggtccagc attatcagca gcattagatg tgaatgttcc ttcaaatgtc
    36181 gcagatgcaa tcaatagttc tgagattggt gcaacgattg cagatggcac actgtcagtt
    36241 ggattatggg gttggctaaa actagatcat ttgaatggaa acgcttattt tacagttatg
    36301 atcatgggag ctatttcagt cattattttt tgtaaattga tgcttgccaa cattacgatc
    36361 aaaatgcctg attcagtacc accagcagta tctaaagcat ttgcagccat tttaccagcg
    36421 acgatcgctt tatacgtcat tgctattatc aattatatcg taggtcgtgt agctgatggg
    36481 caactgatca ttgatttggt gcaaaaatat attgcagaac cattcctagg attatctcaa
    36541 gggttgggag cagtattgat cgtcacagta tttgttcaag tattctggtt ctttggtatt
    36601 catggaccaa atgtattggc accagtgcta gagggaatct ggggacaagc acaactaatc
    36661 aatattgata ttttccaaaa aggttataac ggaaaaactg gtacaccagc cgtgttagat
    36721 gcaatcgaag atggaaaagc ttatatgtgg gtccgcggat cgttcgatgc ttttgcatgg
    36781 tttggcggat caggcggaac gatcgtctta ctgatagcta tcttgatttt ctctaaacgt
    36841 gcagattatc taacagtagc taaattatcc attgggcctg gtatcttcaa tatcaacgaa
    36901 ccagtgatgt ttggtctgcc aatcgtattg aatgcaatca tgtttatccc attcttgatt
    36961 gctccagtag tagcaacagc aattggatgg acagctactt atcttggcct agtcgctccg
    37021 gtatcgcagc aagtcacatg ggtagtaccg ccgttcttat tgtcattctt agcaacagga
    37081 gcggattggc gggcaccgat cgtaacatta gtatgtatgg tcgtgacttt cttgatttgg
    37141 gcaccatttg tcatctctgc aaacaaaatg gaccctgccg caggcgaaca acaataagat
    37201 gaacgtgagg agcgggacgc tttaagtttc gttcctcata atgaattttc gaggaggaac
    37261 tgaaatgatt acaattgcca gtgcaatgca acaagatgaa atcaaagaat tgttagaagg
    37321 atacaatgaa gaaggaatga cttttacatt taaagaaaaa caaggaatca agctgttctt
    37381 tgaaacaagt gcagaagatc cggaagttgc ggctaaaaaa gcaaaggaac taatcaaagc
    37441 acagccttgg ggaacagtcc tttattttca atcaacagct gtttagaaaa tggtttaaaa
    37501 agataggatg gttgagatga taaaaaacgg tttattttta tgtagtattg gttctttgat
    37561 cgtgttgtat agtttgaatc cacattcgca aaggtatgat ttgtatagta tggtcacagg
    37621 tctttttcta acaggattag gaatctattt cttcatgaag gggaaaaaaa gagaagaagc
    37681 aaaggagaaa aataactaat ggaaatcacg aaaaaattaa atattccagc atcgtttttt
    37741 tacgataaag ttatggactc tgtccttttc gatgtacgta aagcgaccgg caaatcagta
    37801 actagaaaac agctgaaaaa tttggagtac gtcaaacaat tctcgcaaaa taaccgggct
    37861 cgcataaaaa tcgaagaagt cattgaaaac cagtcttata agtttcgtac atcgacaacg
    37921 aagaatgaat tcgtcgttca ttatcaaatc aaaccattgg acgattcgac ctgtgaggtt
    37981 cattacacag aaaaaatgga gtcttatggc atgcttcaaa agttgaatga tatgctttta
    38041 ggaacaatct tgatgtattt taaacggaga cgtttcaaaa aaatgctaga tatgatcgaa
    38101 gcaacgtatt aaatcaatga aacatggtac tatttagtta ttgaaaaatg aagtaagcta
    38161 gaaggaattt ttcataggaa gatagatctt tcctgcgaat cctcactagc ttttttgtag
    38221 gaaggggcag caaaatgaca aacaaagtaa gagagctagg tgtatcttcg attgaggaaa
    38281 tgtttgattt agccacttat gcatttaatg gggcagatac acctgaaagg cgagaacgtt
    38341 ttcgtacact tgcggagaat tcttggaatt acggattttt tgatgaggag gatcggctaa
    38401 caagtcaagt catggcgacc ccttttccag tgaattttta cggacaagag tacctaatgg
    38461 ccggcattgg ctatgttgct tcttatccag aagcaagggg acaaggcggg atcaatcaga
    38521 tcatggaaaa gatccttgaa gattgccgtg atcgtaaagt aagcttatct tatctggcgc
    38581 cattctccta tcccttctat cggcgctatg gctatgaaca atcatttgat aaaatcagtt
    38641 atcaactaaa cagtcgtgat gtgccgttta tcaaaaaaaa atctggaaaa ataaagcgta
    38701 tggattttga agatgcaaaa gctgcaatca gaagaattta tgaacagatg ccggaaaacc
    38761 aacgagctgg tctgaagcgc gaagactggt ggtatacgta taaattcaag caaaaaggca
    38821 attaccagtt tgctttgcat tttgatgata atgaggaagc aacgggatat atcgtttatc
    38881 agcttgcaac gtcttccttt attattgcgg agtggggatt tttggatcat gatgcttttt
    38941 gcagccttgt tcgcttcgta agttctcaca atggtgcttt tgaaacattt gaatattcat
    39001 gcggctacgg cggtaatgat caaaattatc tattagaaaa tccatttgct tctgtaatga
    39061 tcacacctta tatgatggcg aggatcgtag atatttcttt gtttttggaa aattatccat
    39121 ttaaaaagag acaagctgca tttgccctac aaatagaaga agatcattat gcgaaatgga
    39181 atgaaggaat cgtggaagta acaatcgaaa atggaaaaaa tcatctctca aaagtagaac
    39241 aaacaaaact cccaatcgtt cgaggaagta tccaacatat cacgcagcta ttattagggt
    39301 atcgtaaagt ggaagagttg gtattccaag aaagaataca ggtgacaaaa gaatatgaag
    39361 aaacgttaag ctcaatcttg ccacagcaac agccaattct taatgactat ttttgataaa
    39421 aaacgggtga tgacaatatt tttgtcacca cccttttgtt tttcatttgg catttgaagg
    39481 aaaatgttaa agtaaatcct cttttttcgt aaatttgaat tttttgtttt cttttgtttt
    39541 agggttttct tttttgtggt cttggattgc ttctttcgta atcttttccc gatttttttt
    39601 agatgaatct aagcgttctg cgtatacttt ttcttttggc ttatcattca ttggaaagga
    39661 cctccctaca ttatttcatt ctacctctag tttatcatct gttaaggact agtggaaacg
    39721 ttttgcctaa cttataagct gattttttta gcagataagc ttgcaagtaa aaaacaaaca
    39781 agctacaatt gatttattga aattgttcct gtagctcagt aggatagagc aatcgcctcc
    39841 taagcgatag gtcgctggtt caattccagt caggaacgta taaataaccc ctgtatcctt
    39901 tgcggtacag gggtttgttt tgtatttgtg ccaaaaatgt gccaaactag ttatcatttt
    39961 tatataaact atcgaacaca gtattgattt tttctttatc ctgatcttca agctctcgaa
    40021 caatatgaag ataagtagac attgtagttt ctaaactgct atgtcctaaa cgtttcgata
    40081 cgctaagtat atttactcct tgataaagta aaatagatgc atgtgtatgc cttagtccat
    40141 gcaaagtaaa ttgcttttca atccctaatt cttttaattt tttgcgcaaa tatttgctga
    40201 cggcgtttga tgaaactaat ccatttttta agttgaaaaa aacaaaatta tgagggtttt
    40261 tgatttcaaa attctcgaat aattcttttt gattgatttt aaactttttt aagagatcaa
    40321 tcgtatgttt atcaatggaa attttcctat ttgaagtttc gttttttgtt tttccccatt
    40381 catttaattt ataattccat gttttattaa tagtgatgat ttgatcttcg aaatcgatat
    40441 cttcccatgt taatcctaga agttcagcaa atcgcattcc agtggcacca gcaactaaca
    40501 cgagcatagg agaagagtac tgcgcagaaa ggttttcttc tacgagtttc attaaagatt
    40561 taaattgatc ataatctaaa tatttatctt cttttggctt caatgaatct tttcctttga
    40621 ttactgcttt tctagttgga tcaaacggaa ttaatccttc ttctacggca tctttcaacg
    40681 acgctctaat atggttatta aacttaataa cggtagattt tacatggtct ttagcatact
    40741 tatttaaaaa gcgttgatat cccactctat ctaaatcaga aatcaatacc gctggcatat
    40801 atttctttat gttcattaac gtatcttcgt attttctata agtgatagga gatactgttc
    40861 cttctttata aagttgcatc caatcctcaa agtaatctga taataataga tttttgcgat
    40921 cccctttaag gcctttgttc aagttatatt ctaattcgtt ggcggcatct tttgcttctc
    40981 cttttgtctt aaatcctgat tttcttatct tcgtgtattt tccgtcatct tttttgtatg
    41041 aaatttcgta ttgccaacta tttccacgtt tgactaatct tgccataatt gaatcagctc
    41101 tctttctctg atacaatagg aactataaag aagcccattg tttgggtttt attatttttt
    41161 gcacatcctt tttgcttgcc ggcgcaagga tgtgctattt gtcgttttaa tggtataatt
    41221 ggatgtgtat tagatcatcc aatcagaggt gtttattatg attaaagtga tttttaaaag
    41281 aataactgaa cttccagagt tt
//
LOCUS       DACDRN010000016        38662 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDRN010000016 DACDRN010000000
VERSION     DACDRN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.016"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            62..763
                     /locus_tag="IWJ80_001101"
     CDS             62..763
                     /locus_tag="IWJ80_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP9460848.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            760..1881
                     /locus_tag="IWJ80_001102"
     CDS             760..1881
                     /locus_tag="IWJ80_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP9460849.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1896..3128
                     /gene="pepT"
                     /locus_tag="IWJ80_001103"
     CDS             1896..3128
                     /gene="pepT"
                     /locus_tag="IWJ80_001103"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP9460850.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3260..4168
                     /locus_tag="IWJ80_001104"
     CDS             3260..4168
                     /locus_tag="IWJ80_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP9460851.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4203..4475
                     /locus_tag="IWJ80_001105"
     CDS             4203..4475
                     /locus_tag="IWJ80_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP9460852.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4486..5532
                     /locus_tag="IWJ80_001106"
     CDS             4486..5532
                     /locus_tag="IWJ80_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP9460853.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5783..8392
                     /gene="clpB"
                     /locus_tag="IWJ80_001107"
     CDS             5783..8392
                     /gene="clpB"
                     /locus_tag="IWJ80_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP9460854.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8543..9214)
                     /locus_tag="IWJ80_001108"
     CDS             complement(8543..9214)
                     /locus_tag="IWJ80_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460855.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9207..9701)
                     /locus_tag="IWJ80_001109"
     CDS             complement(9207..9701)
                     /locus_tag="IWJ80_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9460856.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9721..10941)
                     /locus_tag="IWJ80_001110"
     CDS             complement(9721..10941)
                     /locus_tag="IWJ80_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9460857.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11088..12923
                     /gene="lepA"
                     /locus_tag="IWJ80_001111"
     CDS             11088..12923
                     /gene="lepA"
                     /locus_tag="IWJ80_001111"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP9460858.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            13119..14030
                     /locus_tag="IWJ80_001112"
     CDS             13119..14030
                     /locus_tag="IWJ80_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9460859.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            15026..15277
                     /locus_tag="IWJ80_001113"
     CDS             15026..15277
                     /locus_tag="IWJ80_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460860.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            15360..15632
                     /locus_tag="IWJ80_001114"
     CDS             15360..15632
                     /locus_tag="IWJ80_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460861.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            15722..15961
                     /locus_tag="IWJ80_001115"
     CDS             15722..15961
                     /locus_tag="IWJ80_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460862.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            16108..20391
                     /locus_tag="IWJ80_001116"
     CDS             16108..20391
                     /locus_tag="IWJ80_001116"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAP9460863.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            20490..20855
                     /locus_tag="IWJ80_001117"
     CDS             20490..20855
                     /locus_tag="IWJ80_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460864.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            20857..21309
                     /locus_tag="IWJ80_001118"
     CDS             20857..21309
                     /locus_tag="IWJ80_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460865.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            21923..23233
                     /locus_tag="IWJ80_001119"
     CDS             21923..23233
                     /locus_tag="IWJ80_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP9460866.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            23256..23567
                     /locus_tag="IWJ80_001120"
     CDS             23256..23567
                     /locus_tag="IWJ80_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460867.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            23928..25184
                     /locus_tag="IWJ80_001121"
     CDS             23928..25184
                     /locus_tag="IWJ80_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9460868.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            25181..25726
                     /locus_tag="IWJ80_001122"
     CDS             25181..25726
                     /locus_tag="IWJ80_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460869.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            25727..26227
                     /locus_tag="IWJ80_001123"
     CDS             25727..26227
                     /locus_tag="IWJ80_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460870.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            26308..26864
                     /locus_tag="IWJ80_001124"
                     /pseudo
     CDS             26308..26864
                     /locus_tag="IWJ80_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26884..27228
                     /locus_tag="IWJ80_001125"
     CDS             26884..27228
                     /locus_tag="IWJ80_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460871.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            27247..28158
                     /locus_tag="IWJ80_001126"
     CDS             27247..28158
                     /locus_tag="IWJ80_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9460872.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            28237..29229
                     /locus_tag="IWJ80_001127"
     CDS             28237..29229
                     /locus_tag="IWJ80_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9460873.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            29226..29444
                     /locus_tag="IWJ80_001128"
     CDS             29226..29444
                     /locus_tag="IWJ80_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460874.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            29457..29867
                     /locus_tag="IWJ80_001129"
     CDS             29457..29867
                     /locus_tag="IWJ80_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460875.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            29919..32447
                     /locus_tag="IWJ80_001130"
     CDS             29919..32447
                     /locus_tag="IWJ80_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9460876.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            32463..34673
                     /locus_tag="IWJ80_001131"
     CDS             32463..34673
                     /locus_tag="IWJ80_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460877.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            34681..35772
                     /locus_tag="IWJ80_001132"
     CDS             34681..35772
                     /locus_tag="IWJ80_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP9460878.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            35793..36335
                     /locus_tag="IWJ80_001133"
     CDS             35793..36335
                     /locus_tag="IWJ80_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460879.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            36557..36787
                     /locus_tag="IWJ80_001134"
     CDS             36557..36787
                     /locus_tag="IWJ80_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460880.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRANWNGVVDSVITGSMDSALHGFTGH"
     gene            36801..37133
                     /locus_tag="IWJ80_001135"
     CDS             36801..37133
                     /locus_tag="IWJ80_001135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460881.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            37178..37726
                     /locus_tag="IWJ80_001136"
     CDS             37178..37726
                     /locus_tag="IWJ80_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9460882.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            37733..38185
                     /locus_tag="IWJ80_001137"
     CDS             37733..38185
                     /locus_tag="IWJ80_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460883.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            38282..38617
                     /locus_tag="IWJ80_001138"
                     /pseudo
     CDS             38282..38617
                     /locus_tag="IWJ80_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        13132 a         6047 c         8207 g        11276 t
ORIGIN      
        1 ttattcctag tctccttatt ttatttggaa aatgctataa ttatctagaa ggagtgaagc
       61 tatgaaccat ttagatttat ctcagcgctt agccgcagtg ggtgactttg tccctgaatc
      121 cgctcgttta gcagacattg gatcagatca cgcttactta cccgttgcat tgatattaag
      181 aggaaaaatc gaatatgcag ttgctggtga agtcgttcaa ggtccttatg aatcagcacg
      241 taagcaagtc aaaaaagatg gattagaaga ccgtatcacc gtacgccttg caaatggtct
      301 agatgctatc cagccacaag atggtattac agctgtcacg attgcgggta tgggaggttc
      361 actgatccga gatatcctag aagctggcaa gttgaatcaa cgcttaagcg gacaagaacg
      421 tttgatcctt cagccaaaca tcggagagcg tacattacga tcttggctgc aagaaaatca
      481 gtataaaatc caatcagaaa aaatacttga agaaaataaa aaaatatatg aaatcattgt
      541 agccgaaaaa gaacaaaaac cagttactta caatgaaatg gagctgatgt tcgggccatt
      601 tctgcttgaa gagaaaagtg agatcttcaa atcaaaatgg acaagagagc ttcaacaaag
      661 aaaagctatc cgtgaacagc tcaaacaagc agctaatcaa gaggcacgtc aaaaacaaat
      721 cgagcaggaa atccaaatga ttgaggaggt tttaaagcca tgacaatcag cggacgcact
      781 ttcatccaaa agtttgaaag ctattgtcct cagtggctag ctgaagaagg agacccagtc
      841 ggactgcata tcggaacatt ggataagcca atccaacgag taatgatgac attagacgtt
      901 cgtccagaag tggtcgaaga agcaatcaag aaaaaaatcg atttgctgat tgctaaacat
      961 cctccaatct ttcgtcctgt aaaacggctg gttactgatc agccacaaga aaaaatgtat
     1021 gcagatttgt tgaaacatga tattgccgtc tatgcagcac acacgaacat ggatattatt
     1081 tgggatggac tgaatgactg gttttgtgaa cttctcggta ttgaagtgga gagttattta
     1141 gtaaagactc atgagattca ttataaaaaa ctggccgttt atgtacctgt ggatcatgca
     1201 cagaaaatgc gtgaagtatt agctgcagct ggtgctggta cacaaggtga ttataccggt
     1261 acaagtttta cctcgattgg acacggtcga tttactccgg aagctggtgc acaaccagcg
     1321 attgggaaag tagggaagac agaacaagtt caagaggcaa aagttgaggt tattttgccg
     1381 gaaactatcg aaaaacaagt gattcaagcc atgcggtcag ctcatcctta tgaggaacct
     1441 gcttatgatc ttttcgctat agatgaacca gtcgaaatgt ttgggttagg acgagtaggt
     1501 gaattgccgc aagaaatttc tatagaggca tttgtagaac aagtaaaaga agcgttccaa
     1561 ctagatggac tgagaatagt ccagccaaaa aatgcaaaat cctctgtgaa gcgcattgcg
     1621 atttgcggtg gaagcggtga aaaattttat ccgcaagcaa tcgcccaaag agcagatgtc
     1681 tatatcacgg gagatatcta ttatcatacg gctcatgata tgcaaagcgc tgggttgatt
     1741 gcaattgatc ccggtcatta tattgaatcg ttatgcaagc aacgatttat cgagaaattt
     1801 gaatcttgga aacaagaaga aaattgggat attaactttt tcgtttctga aacaaataca
     1861 aacccatttc aatttaatta aaaggagaga atactatgta cgaaaatctt ttgccacgtt
     1921 ttttacgata tgtcaaaaca gaaacacgct ccgatgcaac aagcaaaacg actccatcga
     1981 ctcagacaca agttgctttt gcgcaagaat tgaaaaaaga attagaagag ttaggcttag
     2041 ctgatgtttt ctataatgaa gataacggat tcgttatggg aacattacca agcaatgttg
     2101 atcatgaagt gcgttccatt ggatttattt cccacatgga tacagcagat tttaatgcag
     2161 taggagtcaa tccacaaatc attgaaaact atgatggcga gtctacgatc gtactagatg
     2221 cggaagaacg ctatacattg aatacaaaag attttccaaa tttgaaaaat tatgaaggcc
     2281 acacattgat cacaacagat ggcagtacat tactgggagc agatgacaaa tcagggattg
     2341 cggaaatcat gactgcaatg gagatcttga tcaataatcc ttctatccct cacggtacca
     2401 tcaaagtggc ttttggtcct gatgaagaaa tcggtgtcgg agcggataaa ttcgatgttg
     2461 agcaattcga tgtagatttt gcttatacag tcgatggtgg tcctttaggt gagttgcagt
     2521 atgaaacgtt tagtgctgct caagcaaata ttacgatcca agggaaaaat gtacatccag
     2581 ggacggcaaa agatacaatg atcaatgctc tacagttagc aatcgatttt cataatcagc
     2641 ttccagccga tgaagttcca gaaaaaacgg aaggatacga aggtttcttc catttgatgg
     2701 cattggatgg gaatcctgaa gaagcttcta tgagttatat cattcgcgat catgatcgtg
     2761 aaaaattcga agcacgcaaa gcacaaatca cacatatcca agaagagttg aatcagcgat
     2821 ttgatcaaga acgaatcaaa gtagatatgt atgatcaata ttacaacatg aaagaaatca
     2881 ttgaaaaaga tatgagcatc gtggaattag cgaaacaagc aatgatcgag cttggtatct
     2941 ctccagtgat cgaaccagtt cgtggaggaa cagatggttc aaaaatctct tacatgggta
     3001 ttccgacgcc aaatctattt gccggaggag aaaatatgca cggacgattc gaatttgttt
     3061 ctttacaagt aatggaaaaa gcaacagacg tgattgtaaa aattgcagaa ttgaacacaa
     3121 agagttaaaa tcgaaaaaga acagcttatg acaaggcgtt cgtcccaatg atggaatgtc
     3181 atctgtctaa ggctaaaggg caggacaatg gaattggttg tcccgccctc ttttttgaag
     3241 agtaaatagg aggtaaagaa tggaaaagct gctagttttt tataacaaaa gttcagggaa
     3301 agatgagggt gagaaattag cacgctggtt tcaagattat gtaaaagaaa agcagccaga
     3361 attatatgta gaactagctg aaactggccc atcgattgat gatgcggaat tagtcaagaa
     3421 agcagaagcc atagaagctg atactttagt aattataggt ggagacggca cgattcatca
     3481 tatcgtccaa gcgttccaaa aaacattatc tcaataccat gtcggattac ttccaggtgg
     3541 aactgtcaac aaccttgcac gtgttcttga gattcccctc aaaaaagaag aagctgctga
     3601 cgtgatcctg gaaggcaatg tacggaaaat agactatggt caagtcaatg atgaagtcat
     3661 tatctctaca ctgacaatcg gaatcttagc agatacagca gtttggatca gtcaagaaga
     3721 aaaacagcgt tacggctcgt ggatctttat caagaagttt tttaagttat tgctgaagaa
     3781 gaaaaaatat catttggatt tagaaacaga cgaggagcat tggaaaggaa aatcccaact
     3841 gttgacagtc acgatgtcta actccgtggg cggttttaca aattttgacg actcagcgac
     3901 cctagatgat ggaaaatttc atgtaacgat cgtaccttcc ttagatattt tccgatttat
     3961 tttttatttg cctagaatca tgagaggcaa attctatacg atccccggca ttacttattt
     4021 cacagccaaa cgcatgaaaa tccaagcgca ggaaaaatct gtgggcactc gaacggatgg
     4081 agacacgaca gatgatcttc cagttgaatt atctgttgta tcaaaaggat taacagttta
     4141 ttcaccaaag aaagaagaaa aagcttagaa atagtagtta acagaaagtt ggaagaagag
     4201 taatggatgt attgttacac aatcctttgt ggttttacct tttcattgtg aatttttact
     4261 tattttgtct gatggggtat gataaacatc aggctagaac tagcggctgg cgtgttcctg
     4321 agaataattt gatatttgtc agcctgttag gcggtggttt gggaggattg atctctcgaa
     4381 aagttttcca tcataaaact cggaaaaaga agtttactat aggattcgct gcaggtgtac
     4441 ttgttgattt gattctcatt tattttttcc attaaggagc aggagatgtc gtctaaagga
     4501 acgaaaacta aaatcattct gaatctgctt ttgctgattg tgatttttgc cattatttat
     4561 tatttaatca atcaatcatt tgcagatatc tttgccgaat tgctgtctac cagtgttgtt
     4621 gttttggcag cggctgttat attagggaca gtttaccaaa tcgctgaagg gcgctcaatc
     4681 aaagaaattg ctgaaccttt cgcgccgaat tttacaacga aagatgggtt cttgacctct
     4741 agctatattg cgttttaccg aatcgtttcg tttggtacag gaacgctgat ctcggagatt
     4801 tatttttata acaaaaaagg aatgaagcta tcccagggag caggggtgac agcactccat
     4861 atgattatgt acaaactggc agtgatcact tatgcattag ctggcttggt catccaattc
     4921 tctctttttt attctaatgg tccatcaatg atctggttta tcatcgctgg tatccttttg
     4981 acgatcgtga ttgttgcctt tttattggcg atgtccgtca gcttgaatgt tcaaatattt
     5041 tttgtcagct tatcgaataa gttatttaaa agtgagcgtt tgcggaaatg ggtggataca
     5101 tgcaatcagc agatctactc tttaagggaa acggtccaaa ccattatcca agacaggact
     5161 gcgctattgc ggatttatct atggaatatg ttaaaacttg tattttggta tgtacttcct
     5221 tatttgattc tgattgaaaa ccatcctaaa atagattttc tactgaccat ttcgtttatc
     5281 agctttgcag tagtgctttc aggggttatt cctacgcctg ctggtattgg ttcctttgaa
     5341 tttgtctatt tgttattatt cagaccagtt gttggaacag ttgacgcagt ttcttcgctt
     5401 ttactgtatc gtttcagttc ctttatcttg ccatttctct atggatttgt gtatgtgatt
     5461 gctgaacggc gaagagtgat caaagaagaa ataatagaag taaaaaaaga gaataaagaa
     5521 aatcaagggt aggtctttta taagatctac cctttttatc aaatcaaaga aaaacgttat
     5581 ggataagata attttacgtt tgtttggctc tttttttata tacatacaat caattttgtt
     5641 tttttaagat ttatataagt cgagaaaaaa agtcaaaaaa ctatgtgtat attttgacca
     5701 aaattgacca aagcgatata atagtttttg taaagatcaa agaaataagt gatcttacct
     5761 ttaattagga gggtatacct gtatgaatat tgagaaaatg actacgacgc ttcaagaagc
     5821 gattgctgaa gctcaacaga ttgcggtcac tcgaaaacat caagatatcg acattgccca
     5881 tgtatggaaa atctttttac aatctaatca ttttggtcga aatttctata cggatgccgg
     5941 attggatgtc gaatcttttg aacatgaaat tgatcgtcta ctggatgagt atccagttgt
     6001 ttctggtgga aacgtccaat atggacaaaa cctcagccag aatttctttc gattattgaa
     6061 tgaagcagac caaatacgtg aaagcttcgg cgacgagttt ctttcaacgg aagtcgttat
     6121 tttagcgttg atgaaattaa aaaattatcc gttaactgtt tatttgaata aaaacggatt
     6181 atctgaaaaa gaattgcgta aaaacatcga agagatgaga ggaggagaaa gagtgacctc
     6241 acaaaaccaa gaagaacaat acaaagcatt agaaaaatac ggcgttgatt tagtacagca
     6301 agtcaaaagc ggaaaaatgg atccaatcat cggtcgtgat gaagaaatcc gtgatgtcat
     6361 ccgtattctt tcacgaaaaa caaaaaataa tcctgttctg ataggagaac ccggtgtagg
     6421 gaaaacggca atcgttgaag ggcttgctca acgtatcgta cgcaaagatg tgcctgaaaa
     6481 tttaaaagat aaaacgattt tttcattaga tatgggtgca ttgatcgctg gagcaaaatt
     6541 ccgtggtgaa tttgaagaaa gactaaaagc agtcttgaaa gaagtcaaaa aaagtgatgg
     6601 acgtatcctc ttgttcatcg atgaaatcca caatatcgta ggggcaggaa aaacagaagg
     6661 aagtatggat gccggaaatc tgttgaaacc aatgcttgct cgcggtgaat tacatttgat
     6721 cggtgccaca actttagatg agtaccgtca atatatggaa aaagacaaag cgttggaacg
     6781 tcgctttcaa aaggtattag tcaaagaacc aacagtggaa gatacgatca gtattctgcg
     6841 tggattaaaa gaacgttttg agatccacca tggtgttaac atccatgaca acgcattagt
     6901 agcagcagct actttatctg accgttatat tactgatcgg ttcttaccgg acaaagcaat
     6961 cgatttgatc gatgaagcca gtgcaacgat tcgggtagaa atgaactcta tgccaacgga
     7021 attagatcaa gttactagac gtttgatgca gttggaaatc gaagaagctg cactgaaaaa
     7081 agaatcagat gatgcaagta aaaaacgctt gaaaaatttg caagaagaat tagctgaact
     7141 tcgtgaagaa gcaaatgcga tgaagatgca gtgggaaaca gaaaaagaag aagtcaattc
     7201 tgtttcagca aaacgtgctg aaatcgataa agcaaaacac gaattagaag atgcagaaaa
     7261 taactatgat ttagaacgcg ctgctgtatt gcgtcatggt acgatacctc aattagaaaa
     7321 agaattgaag gaattagaag ctaaagcaaa agacagcgag attaagatgg ttcaagagtc
     7381 cgttaccgaa aatgagattg ctcaagtcgt tggcagatta acgggtatcc ccgtaacgaa
     7441 acttgtagaa ggtgaacgag aaaaactaat taaattgaat gaaacacttc ataaacgagt
     7501 gattggtcaa gatgaagcag ttgatgcggt cagtgacgcg gtaattcgtt cacgggctgg
     7561 attgcaagac ccaaatcgtc ctctaggttc tttccttttc ctaggaccaa ctggtgtcgg
     7621 aaaaaccgaa ttagcgaaag cgttagctga gaacttgttt gactcagaag atcatatggt
     7681 acggatcgat atgagtgaat atatggaaaa acatgctgtg tctcgtctag tcggagctcc
     7741 tccaggctat gtaggttacg aagaaggtgg tcaattaacg gaagctgtca gacgaaatcc
     7801 ttatacaatc gtcttgttgg atgaaatcga aaaagcgcat cccgatgtct tcaatatctt
     7861 gttacaagtc ctagatgacg gacgcttgac tgattcaaaa gggcgagtag tagatttcaa
     7921 aaatacggtt ctgatcatga ccagcaatat cggttcacag ttactgctgg aaggcgtgac
     7981 tgctgatgga acgattccag aagcagtggc tgaacaagtc aacacgctat tacgtggaaa
     8041 cttcaaacca gaattcttga accgaatcga cgacacgatt ttatttacac cattgagcct
     8101 agacaacgtg aaaggaatcg ttgacaaaat ggtggcgcaa ttggcacaac gtctggaaca
     8161 tcaagaaatc ctgctgacaa tcagtgatga agccaaaacg tggattgctg aaaatgctta
     8221 tgaaccagca tatggtgcaa gaccattgaa acgatttatc actaaggaag tagaaacacc
     8281 actagctaaa gaaatcgttg caggacatgt tatgcctaaa tcaaaagtaa cgattacttt
     8341 acttgatgga caacttcatt tcaaaacaga agagctagaa gaaatcgtct aatcttccat
     8401 tgagactata aaagaaaaaa ggggtctgac acaactagtg tcagacctcc ttttttcaaa
     8461 taaacagtgt tccagaagta gtttcttcga aaaataagcc gaaatttctt gaagctgccc
     8521 gcttctgtca caacctcttt tattaggtat ttttcacttt ttctaattta taactttcaa
     8581 agtaacgctc atctttttta ctttgagacc atgcaaacaa tcccattaag gcagcaccta
     8641 tcgtatttgt cagcaagtcg cccatcgtat ccatcaatgc tgctcgtccg acaaacagtg
     8701 tgccatcaga agcagcaaaa cgttgaagat tcatgcctaa gaactggtcg cataagaatt
     8761 cccagaactc ccaaaacacg ccgcaaagtc cagcaaacgc aaagccgaat aagaggaaca
     8821 accaaggact ggtttttgat atctcagcat ctttcatcaa gtaaccaatc aatccatagc
     8881 caagagctgt caggaccatt ggactcaccg catgtaagat tttgtcccaa aaagagatga
     8941 tactgatcaa atgcatccct gtgcctaaaa agacagagat gaacaagaaa aaccagtaaa
     9001 acaaaacgat tgctgctggt attttaagtt tgaaaacttt caaaatgact tgtggcatga
     9061 aaatcaatgc aatccctaat aaagattcca cgataaatgt gattccttga gaagtcgaat
     9121 atttttgtgt gataaattca taaaaattat agcagacaac gaatacacca aaagcaagta
     9181 aagctatttg cacctttttg atttctttaa tcactgaaag tccctccatc taaaaatctt
     9241 tttacttctg aaagcaactc aatttctgaa gaaaccactt ttccgttcaa tttgatcaaa
     9301 cctattgtat ataagttcag gtagtgaaat tgattttcag ctgtttcttg tagtgcagcg
     9361 atttttttcg ggtgggttcc gccttgctgt cgagtatcag tatataagcc gatcactggg
     9421 atgcctttcg cataagccac accgatttcc gatgctaccc cggcatcgat cgtcagccca
     9481 tctagtaaag caatcattaa atcactagcc aacacttttt ctgtgtcagc aagagcaatc
     9541 attcgactgt ctgcataagc agttttgtca ttgattgctt cattttcttg aggaagatag
     9601 atcgaaagct cagaagagag ctctctgatt tctttaacta actgttggtt atataacaaa
     9661 tcacttttag caaacattgg tgcggcaaaa taaattttca tctaaacatc atccttttta
     9721 tcaaaaataa tcgctaaagt aagcttccat gtttggaatg atacgctcta gtgacttcag
     9781 tgtttcttca tctgtttcta accgttcgat tcttgccaaa taagaagcac ggcgataatt
     9841 catcatcaag caagtcaatg tttggatatc cagtcgaacg gattttccta taggttcatt
     9901 taagacacgg acttgtccct gttcatccca tgttaaagcg aagacaccat tattccaagg
     9961 agcgacagga tcttcaatga tgaaatgaaa agcatcagct gttccaacaa acggaaaccg
    10021 ttgaaggaat tcttttacat caacgatccg agccataaaa tatggttcga tctgctcttt
    10081 gatctgacta tcttcaatca aaaatgctaa aggctcattt ttataaatat ctccatgtac
    10141 ccagtagatc atggaaaaat gtgcggaaat aaagttccat aagccatttc gagcttcttg
    10201 atcaagatag aacatttctt ttacgtgaaa tatttcttca gctacccagt agaacagaac
    10261 acctcttggt tcatggttgc tatcgtagta gacagcggct gtgcgctcat cttcattttc
    10321 gaaacgccaa tactcttccc aatgaaaatc actgcggaat aaagcaccat ggttttgttg
    10381 ggcaaatttg tcataaacgg aaaatacgtc tggatgatca actggtaatc gctcaaccat
    10441 accacttact gggatttgtt taggtagctg tgtatctctg attttaaagg aaagtttatc
    10501 agacatgatt tcccatcctt tacgccgata gtaggggata ctgtaaggat acaaataaga
    10561 gatccattgc ttattcttgc gcatattatc caatgcaacg ataattagat cttgcatcaa
    10621 tccatggtta gcatattctg gataagttcc aactccagtc acaccaccca ttttataacg
    10681 tgcaccgtga atattgactt cacaaggata gatagcgatt tgagagatca gtttattttc
    10741 gtggaaccat ccgaacactt tcgatacttc caaaatcggt tttttggaac ggataaaagc
    10801 tcgtttattc tcaaacccgc tttcctcgat gtctgcttca gtcacttgga acacatagga
    10861 aagcaattca ttgaattgat cgatatgttc ttgagtgacc ggttttaatt caagattttc
    10921 tctaaaatct ttttgatcca ttttttcatc tccatttatt agcttgataa cagtatagca
    10981 aaattctaca aaaaaaactt ctatagctga ctaattaaaa actctttaaa ttactgcttt
    11041 tcgttggtat aatggaaagt gctgattata tgaaaagagg agacgctatg gatataaaga
    11101 aaatgaaaga acgccaagag cagattcgta atttttcgat catcgctcat atcgaccatg
    11161 ggaaatctac attggccgat cgtattttag aaatgacgca cactgtgacg tctcgcgaaa
    11221 tgcaagatca gctgttagac tcgatggatt tggaacgtga acgtggaatc acgattaaat
    11281 taaatgctgt agaactcaat tacacagcga aaaatggaga aacatatatc tttcacttga
    11341 ttgacacccc agggcacgtc gacttcacct atgaagtctc tcgaagcctt gctgcctgtg
    11401 agggtgccgt tttagtggtc gatgcagcac aaggaatcga agcacaaaca ttggccaatg
    11461 tttatttagc gttagacaat gatttggaga tcctgccagt catcaataaa atcgacctac
    11521 ccgcagcaga tcccgaacgt gtccgtcaag aaatcgaaga cgtaatcgga atcgatgcta
    11581 gtgaagctgt attagccagt gccaaatcag ggattgggat tgaagatatt ctcgagcaga
    11641 tcgtggaata tgttcctgca ccaagtggag acttagatgc accgctaaaa gctttgatct
    11701 ttgactctat ttatgatagt taccgcggtg ttgttctaaa tgtccggatc acggacggta
    11761 tggtcaaacc cggagacaaa atcaaactga tgagtaatgg caaaacattt gatgttacag
    11821 aggtcggggt cttctcgcca aaagcagttg ctagagactt tttgatggtc ggtgatgtcg
    11881 gttatatcac agcaagtatc aaaactgtcc aagatacacg agtaggggat acagtaacgt
    11941 tagcagacaa tcctgccgct gaagctttac caggttaccg aaaaatgaat ccgatggttt
    12001 attgtggtct ttatccaatt gatacttctc gttacaacga tttgcgggaa gctttagaaa
    12061 aactgcaatt gaatgatgct gctttacaat tcgaaccaga aacctctcaa gcattaggtt
    12121 ttgggttccg ctgtggattc ctcggacttc ttcatatgga tgtcgtccaa gaacgcttgg
    12181 aacgggaatt caatctagaa ttgatcacga ctgcaccatc cgttatttac catgttaata
    12241 aaacggatgg tacgactgct acagtagata acccggcaga ttttcctgaa cctgtaacga
    12301 ttcaggatgt agaagaacca ttcgtcaaag cacagatcat ggtcccaaat gattttgtcg
    12361 gtgcagtaat ggaactttct caacgaaaac ggggagaatt cattacgatg gattatttag
    12421 atgattaccg agtaaatgtc gtctataata ttccgctttc tgaaattgtc tttgactttt
    12481 tcgacaaatt gaaatcaagc acgaaaggct atgcctcatt ggattatgaa atgtctggtt
    12541 atcaaaaaag caagttagtg aagatggaca tcttgctgaa tggagaaaaa gtcgatgcat
    12601 taagctttat cgttcaccga gactttgcct atgaacgtgg aaaagcaatc gttgaaaaac
    12661 tgaaaaaact aatcccacgc caacaatttg aggtgccaat ccaagcagct attggacaaa
    12721 aaatcgttgc ccgttcagac atcaaagcct tgcgtaaaaa cgtcttggct aaatgttatg
    12781 gtggagatgt ttctcgtaaa cgtaaactct tagagaaaca aaaagaaggg aagaagcgga
    12841 tgaaacaaat cggctccgtc gaagttccgc aagaagcctt catggccgtt ctgaaaatgg
    12901 atgagcaaga tagtgtgaaa taacttataa tcaatcgaaa aacctattaa ataaaccttt
    12961 ttgagtagat gcaacactga ggattatctg tataaaagat tttaaactgt catttggtta
    13021 tcacggcgat actaaaatta acaaaattgg aatatttttc ttgaagattt ttagctgtca
    13081 gtatatgatg aaagcatctc ataggaggtg cttttttcat ggttgataat tcaagtggaa
    13141 gggttcttaa atctctaaga aaagagaaaa aactatctca aaaaaagtta gctgatttag
    13201 caggtatttc acaaagtact ttagttaaat atgaaaaagg gtcaagaaaa atccctaaag
    13261 acgtagataa tactctaagc aagattctga atatagaaac tctaataaaa gatgaggaag
    13321 ataagattga gattctcatc ggtaaactaa ttgcatacag agatatgaat aagttactta
    13381 acaaagagtt agctgataat ataggaatta gtgaagtatt actcagttat gtgttaaata
    13441 gaaagagaaa tccgtcaaaa gaaatgcaaa aaaaaatcga tatttttcta ttaagtaatg
    13501 aaaaagaaat tttgaaggag attaatcgtg atagtgagat atttagttta tcaaaagacg
    13561 ataaaattgt tatgggaaaa agaatacgag aagtccgaaa gaaccgggaa gagacgcttg
    13621 aaaaatttgg taaaaacttt accatctata caggcaaaaa tgttataagt agatgggaaa
    13681 aaggtattaa tattccagat attgaaaaac taatgaatat tgcctatctt ggtaaggtca
    13741 ctgttcctta ccttatgtac ggagaagatt ataagaatat acttccaaaa gatgaaagag
    13801 ttagcgattt taaaaagata aattctttta gtatgggatt aaggatgagg aaaattagga
    13861 aagattatta tctagaaagg gaggagtttg gaaagctttt ttctccttca atttcaaaat
    13921 ggtccataga taggtatgag aatggtagag atatacccaa tactaatcga attatccaat
    13981 atgcttatat aggaaatctt tctttagaat ttttgattta cgggatataa aaatagttta
    14041 cgtcgatact tattaagtta tcgacgtttt ttattataga aaaaatttat aaaaggaggt
    14101 ttcttgatat gataagaaac aaaaagggtt tgttgaaagt tgctttactg gcagcttttt
    14161 tctaagtaac tttttgacaa gctcaatggc aacacaagcg gaagaagtaa tacaagaact
    14221 aactgttatg aataacagct agataaaatg ggctatgtat gttatccaac aatttagtaa
    14281 tcactagaag cctagcgaaa gttcgcttga taagttatag atgagtattg atttgtagtg
    14341 tttccaaatc aaaagggtac agagccacat gaaatatgtt gtcctctgca gcgtcaggat
    14401 gggaatccat accatggaga ttcggagatc tgtgagggaa tacttacgtg gttaatatgt
    14461 cagttgtgtt ggacgtagta atggtcatag tgggtagatt gactgaattc ttatatgaga
    14521 aaatgaaacc aacaagaaga tagggttatt tatacagaat tggggatctt ttctggaaaa
    14581 gtaatcggat aatgcgagat tcgtacggtc ttttttaaat ttctctaaaa aaaacggcat
    14641 cgaatgggtt gggtgattga taagcaatga atcatagact gatatagaat aaaaaatgat
    14701 tctatttatc tttttacatc tatcgttttt tcaagtagcc gcaaattata tcagcattca
    14761 acaatttttg tatacaagtt ctagtaaaag gatctgaaga aaaagttggt tgttgtgaag
    14821 ataaaaaaag aatcaggttt tactgagttt ttcttcaaaa tttgtgttat taggttagga
    14881 caaagataag aagaagtatg aagatttaaa aaaggaatga ggaaaagata gtaatttccg
    14941 attattggag tttttttatt tataaagaag aagcgaataa gttgtgattc ctagatttcg
    15001 cgcgtgatta attaatgagc aaaaaatgcg gaaagtttta gtgtttaata cacgaggaca
    15061 aagtttctta ggtattgaag gcacacgtgt aacggttgat gggtggtgtg attgtttcga
    15121 ggttggaaaa catgttgaac ttatggcatt tactgagcta aaagataaga acaacgtgga
    15181 atttttcgag agtgatattg gttgggacga tcatgaagaa gtttatggaa aatttatttt
    15241 gcggaaggga aattcttata cgaatgggga agcataagta gcgatttgtt agagatcatt
    15301 gataggattg aaattgtcgg gaatatttat gagaatctag agttatttga ggtgatagaa
    15361 tgagtgatgg ttttgaacaa gtatcaataa ttaaaaatca agttcgggag attcttagaa
    15421 aaaaaggcta tttggtagat agttattttg aaggagatta cgaaacatgg gtaggtgtct
    15481 atgctcgtcc agagaataaa cctacctatt tagatcctac gacgtcagaa gatgcctatt
    15541 tacaaaatag gtatcgagta gatggtttta agcaagactt tgcggagtgg tttgaatggg
    15601 aaattgaaaa cggagaagta aaagaagaat aagagtactt gttgtatcaa aataagccag
    15661 tagaacaacc aaaccagtag ctagtaatga caataatact gaggaatcgg atcattatgg
    15721 tatgattgat atttctcttc cagaagatct taagccagat cctgtaacaa ttggcaatag
    15781 tgtcatgatt tttgattcat tagatgaagc tatgtcatat ggttggaaag aaattaagga
    15841 tgaaggtagt aattggtatg gcatgagatt tggtgatggt gaagttgcca aaaaagctgg
    15901 tattccaatt gggaaatgga cagttgattt ccacgaagca ggttcttttt ttgttctgta
    15961 attatagtgg gagggtaaaa tcattgatga aatgtgttca gataagagcg ccagataaaa
    16021 atagaagaga cagttcacgt cgttatcact tttggtaacg gcgttttgtt ttattatata
    16081 aaattctcag gaaaaggagg aattcttgtg tatgcaaaaa cgaaaatgaa ttggttgaaa
    16141 gcactcgcat tggtggcttt ttctcaattc tgtttcttgg tgggaactgc tcaagcacat
    16201 gcagaagaaa cgacggctat acaacctacg atggaagcca ttgcccaagt cgcttctgaa
    16261 gtgacagaag tccaaccagc agaagccggg gtcccagctg gtgaagccac tgttgaacag
    16321 gcccctgtga cagaagcgcc aacggtcaat tccaaaacga ctctcgatca gacgattggg
    16381 gaaacacaac acccagggaa tagtcctggt acttcacaac cagttgaaga aactgcctca
    16441 attacaaccc ctgcttcaga aacgtccact ctagaaggag aaaaagctgc cgaagcaacg
    16501 ccttctgtgc agccagagtc ttctgagaaa caagcggaag aaaagaaacg aatagaagaa
    16561 acagtggaac aaccagcggc tactgctgac aaagaaggaa aaaatgagga aaacgttgaa
    16621 aaaacaatct cttcaaccgc agctagtcaa aatcaaccaa cgaaaccaaa caaagaatct
    16681 acggctagta ttgaaaatca aaagaaaaaa gagcagaaca ttccaaaaac aggtacacct
    16741 acactgttag taacagtacc aaccaatcgt tcggttcgtg ttcgccgtgc aaccaatgca
    16801 gctgttttag gtggtcggaa aattgattgg ggcgaaaata ttggtgttac ttctagtccg
    16861 aacaatctaa acttcacttt aaattatatg gttcgttttg agattaatgg acaagtggcg
    16921 ttttgtattg agcctggtgt ctcagccgca gaaggtcaag tttatacagc tactgctttg
    16981 aaaaactatt tgaaaaattc aaatgtacgg aaaaaaacaa gcttgatctc ttatttagga
    17041 tatgtcacaa ataaagacaa atccgatgaa caatacattg cggcacaatt tatgatttgg
    17101 gaagctttgg gcacaaaaat cccaatcaaa aatatccgtg ttgattatga tcgtcgaaaa
    17161 gcagaaattc aacgtttgat taataacttt ggtgaaaaag caacttttaa taatcaaaca
    17221 catacggtaa aaattggcga aacgttgaaa gtaaagaata aaaatgattt tttgaaatat
    17281 gtgaaagaca ttcaaaaacc aaaaggtgtc aatgtatcta ttcacggcaa tgaagtatgg
    17341 attacagtca caaaagaggc accggataaa gcaaaaattc aattgaataa aattaaagtt
    17401 caaggtgcgc cattagccta tcgtcgtcca ggagctcaaa caattggtgt gttacatcca
    17461 acagaaccta atgcgtctgt tattaattta caaattctaa aacaaggaaa cattcgtgtg
    17521 tttaaagaag atgcggaaac tggtgtgaaa gcacaaggtg cagcttcttt agaaaatgcg
    17581 gtctatggtt tgtatcaagc agatgggaaa aagattaaag aaattacctt gaaaaaaatt
    17641 aacggcaaag tacaggcaga aattaaagat cttgatcctg gcaaatatgt tttaaaagaa
    17701 atcaaagcgc caaaaggtta tgtgctttct gatgaagcaa ttgaaattgt tgttgaacct
    17761 ggaaaaactg tcaatgcgac cgcaaaagat caagtgatca aaggaaccat tgatttagtg
    17821 aaagttgcta ataaagattt agtcgattca acgaatccag ataacaaacc aaaattagct
    17881 ggcattgaaa tcactttgac gtcaaaaaca actggcaaag tggtgaaaac cgttgtgaca
    17941 gacaatgatg gttatgctac ttttggtaaa aataccgttg tctttgatac gtacattctt
    18001 tctgaaacca aaggaaaaga agggtataaa ctatttgaac cacttgaagt cacaatttct
    18061 gaaaatgggc aaactttcca ttatgtttta gaagataaag taattgaaca acgtcttaaa
    18121 gtggtgaaag tggatcaaga aacaggaaaa gtgattccac ttgctaatac agaattcaag
    18181 atttttgata ctttagccaa tcgttatgtc acaatggaga ttccaaatga tacagaaaca
    18241 acggatatct ttaaaaccaa tgaaaaaggc tttttcctaa ctaacggtac acttacttat
    18301 ggaaaagatc gctatcgttt ggaagaaatc aaagcccccg taaattacgt tttgaatcaa
    18361 aacccattag tgttctcgat taccaatgat acagaaccga tcaaagtgat ccattttgcg
    18421 aaccgtcaag cacggaaaag tgtaaaatta ttcaagtatg aagaatggaa caagaagaaa
    18481 acgccgctcg caggtgtgaa ctttaccttg tataacggta aaaatcaagc aattggtgaa
    18541 tataccactg atcaaaacgg ggaaatttta gtgaaaggtc tagttgccga tgactattac
    18601 ttcttggaaa atgccccatt agaaagtttc caaccaaata cagataaaca agcattcacg
    18661 gttgcattca aacaaaatgc gccagatgat ggggaaacgt tagtggtttc tgtacgtaat
    18721 tacttgattc caccaaaact agaaacacaa gcgtcaaata aagccgatgg tgaaaaagtg
    18781 gttgatccat tagaaacgat cgtcattaaa gacagtgttt cttataccaa cttgtttgtc
    18841 ggaaaagaat acaccatttc aggagtattg atgaacaagg caacgaatgc gccaatttta
    18901 caagatggca aagaaatcac aagtaccttg gcctttattg cagacaaagt caatggcaca
    18961 aaagaccttg aatttgtggt taatgctagt gttctgaaag gtcaaacgat cgttgttttt
    19021 gaaaaacttt tccgtgatgg cattgaagtt gcagcacatg cggatatcaa cgatgaagcc
    19081 caaagtgtga aagtgaataa tccaaccgtt cataccacgg ctgtctttga agacggattg
    19141 acagtcgcag acccaacagg attgtttacc ttaaacgata ccgttgaaat taccgagatt
    19201 cttccaggaa aagcatactt ggtcaaaggg atcttaatgg ataaagcaac cggtgaaccc
    19261 ttgcttgtgc atggaaatac ggtagaaacg agtgttggct ttattgccaa agcagaaaat
    19321 gaaacggtga tcgtaccatt tacctttgat ttgacaggta taaatggtcg tgaaatcgtc
    19381 gtgtttgaaa gtctttttag aaatgatgaa gaactagcaa accataaaga catcaacgac
    19441 aaagggcaaa cggtacgagt aactaaccca agtgtacgta caacggctac acataaaccc
    19501 actggcttga aagaggttaa tccattaacg aaagtcacca ttacagacgc tgtgaaatac
    19561 cacgatttgg tcgttggaaa aacatacaca gtcaacggtg ttctaatgga caaagtaacg
    19621 gaaaaaccag tgctagtaga tggaaaagaa gtcacaagta ccttaacgtt tgtggcagaa
    19681 acaacggatg gtacagtcaa tctagacttt attctagacg cacgtgcgtt gcgaggcaaa
    19741 gaagtcgtgg tctttgaaga tctgtttaga gaaggcgtat tattggcttc tcacgcagat
    19801 attaccgaca atgaccaaac catcaaaatc agtaatccaa ccattacgac gaaagcgacg
    19861 aataaaaatg gcggaaaaga gatcttggcc ttgccaaatc aaactgtgct tgaatgggtc
    19921 aaagtagacg gtcttgcgat tggtcaaatc tatcgcttaa ttgttcaagg ctacttaaca
    19981 ccagacggca caccatttga aggcactcat gcggaaaaag tctttgcggc cgataaagaa
    20041 atgatggaat tcttatttga attcttagtg gacggtcgca atcttgcagg aaaaggcttg
    20101 agttttgcgg aatggctcca agcgaaaacg gaagatgaga aagaaacgtt tgaagaagtg
    20161 gcgaaacaca atgtagatct aacaaacaaa gatcaaacgg ttcaaattgt ggaagcgcca
    20221 aaaccaccgc aaccagtgaa accagaacaa cgggttaaac cagctactgt tgtaccaatg
    20281 ttgccaaaaa caggtgaaca aacaagtagt atacttatgg ttcttggatt agtagtatta
    20341 gtcattgctt taggtataac actttataaa gtacgtaaag ccaaagatta gtcatgagga
    20401 acaagcggtt aggattttta accgcttgtt tgattgtaat gtagaaatat ttagaattta
    20461 ttttggtttt taagaggagg aaaaacttta tggcagaaaa agcattgcgt atgaagaaag
    20521 agtttttagg tcaagtattt ttattgggtt cacgggaacc taatccacca aaggaaatta
    20581 atggtgtgtc tgggcgatat gttcaattac aagcagagtg ttcgcgagaa gaacagcctg
    20641 ctttttttgt ttatctcaca gctgaagagg ataaaagatt acgattacca gaaaaagagg
    20701 tgcgtatttc atttgaagaa gcttattttg tttttcgtaa agaacgagtg aatagtcgtg
    20761 aaacaaatga tatgggacaa acagagcgtc gaacaactgg ctatcaagat gttggtgtct
    20821 ttttaactgg tgtaaaaatt ggggaggttt tgtaagatgg gattaaaatt tccaaataat
    20881 tatgacatta cagaagcatt agatgaaact tgtttaggag ccgtacgctt tggcgcatta
    20941 gaagagtctg atcgtgtatt tgataatgaa acaggcgatt atacgggaga aattcgatca
    21001 cagcgagcac aattagtttt tggaaatcca gatgaaccaa agattgcacg acgagttaaa
    21061 tttccagcag atgtagattt gactggtttt aaacgacgtg atcctgtgaa attaattgat
    21121 ccagaatatc atgtggtata tattccagca caagatggca atcgtgaata ttcagattta
    21181 gtgatttatg cgaaaggatt agagaaagta gaagctccaa caaataatcc taatatgaaa
    21241 caatcacaag aaaaaggttt agatcaaaaa caaccaagtg ttgattctaa ggaaaaagag
    21301 aaaaaataag gtttttttga aagaatagga aggagtcttc ctattctttt tatctaagat
    21361 agaaggaagg ggagattttg aattcagata aaaccaatca aatactttga gagattgtgc
    21421 ttgttatcaa aatatccaac aattgattga gtattttgga gacaatccaa cgatttttgt
    21481 acaaacaaat gcatatattt gtgcggttat tgggcagtgt atgtttttac gtgatcttgt
    21541 tcgtttttca ttaccgtacc agtggaaaca agatcatcag gaagtgattt ggattcgtat
    21601 agtacgaata tgtaatatgt gtgcacaccc ttataaaaat cgattaaatc tttttcgatt
    21661 atgttttttt actaaatgtg atattcctgt attagttgct ttttgtcaaa agtttttagc
    21721 acaggaaaag taaaaacaga aaggaggaat gtgacaattg aaagctcatt ttttcaagta
    21781 tcgtggtcgg cgaattcgct atttcaatcg ttatttatgg ttttgctatt atggtttctt
    21841 tttgtttcca tttttgattc tgttaggttg gtttagttgg aaagtatttt atccacgttt
    21901 aagtacgttt tcgaattggc aaatgtatct tattcctgga ataagtattg tgttgtattt
    21961 gttgctggtt agtgggttaa ttttagggtt gatgcattgg tcgcgactaa aagaaggata
    22021 ttttgcctct gtttattggc gtcagttact tgttcgaatg ttaattgaca atcggtttgt
    22081 ttatacaaag aaacaagtta gtgagaaaca aacaagggaa aaaatgagat taccaaaagt
    22141 ttatttttat caaaagaaac aagaattagt ggtttctttt cctttagatg gtgggcgttt
    22201 tcacgaacga tttttaaaaa tgggtcattt gttatctgaa gtatttttgc gtgatttaat
    22261 tcgtgaagaa cgagaaaaag cccgaatcaa ttatgtattc ctttcagatt ctggtcggat
    22321 tcctattgat caatgtatag cagaaaatgg acgtattcat ttaatgaaaa ctttggattg
    22381 ggtatatgat caatcaccaa atatgttgat tgctggtgca atcggtggtg ggaaaaccta
    22441 tttattgtat tctttaattc aagcttgttt aagtgttggg acagtagata tttgcgatgg
    22501 aaaagcggca gatttagcag cattaggtga cgtcgatgta ttcaaaggac atgtgtttta
    22561 caaaataaat gaagaaatga ttatttgttt acgaaatgca ttaaaagaaa tgaatcaacg
    22621 gtatgcctat atgaaaacca tgaaagatcc taaatatgaa ccagggaaaa attatgctta
    22681 ttatgggtta ccaccgcatt ttatcttttt tgatgaatgg gcagcttttt atggttcgtt
    22741 ggatatggaa gcgagaaata gagtcgataa attgattcag ccattagtat taaagggtcg
    22801 tcaagcaggg atgtacatta ttttagcaat gcaacgacca gatgcagaat attttccaag
    22861 tggtgttcgg gataatttaa cattccgtat ttctgttggt cgattgtctc ccattggtta
    22921 tttaatgact tttggagaag attttaaaaa taagccattt tacaacaaag atcaacctgg
    22981 tcgtggatat gcttttccag gatttgatgt acctgtagag ttttttgcgc catttgttta
    23041 caaagattat cactttatca aagaatttga gaaatttact gaaatgattg tctatgattt
    23101 tacagtaaaa gaggatagtc aagatcaaac gatgggagat gataatcagt tgttacaaga
    23161 acaactagag cgccaaatgt atttagcaaa agatccaaaa ggaacaagtt tttctgatgc
    23221 gcatattttg taatcaacta aggaggtgaa gttaaatggc agagagttgt tattatagag
    23281 tagctacagc aatacgaatg attaatcctt ctctttcttc cagaacgttt tatgattggt
    23341 tgaatcgtat tgaacaagta actgattacc gctttttgcg caaagaacgt gtttttacag
    23401 gaaaagttat taatcaagta ttgctaacga aaaaagatat agaacgattg actcgattgt
    23461 atcattatcg agtagattta gaagaggatt taactttgtc aatttatcgc gttttttctc
    23521 ctgaaaaata tagtgaaata acaaaattag atcatttaat tttgtagtta ttatctatcg
    23581 ggagggctct agacgtagtc tggtagccgc actctgcctg gcggtgcgca ttaaggctgc
    23641 gaagcatggg cctataatgc gcactcgcta gcagcgtgtg cagctcacaa agaggtcgtg
    23701 cgcttgcgca cggcctcttt gtgtaaagcc ccccgatata taatatgggg ggcagaaata
    23761 acataacaca tagaaaaaag ttttataaac ccttttgtat caagtgatta tctaaggtca
    23821 gcagttgtac tacttttccc aaaaaacgag ggggactagt tgatatatcg gtgtttctaa
    23881 agggttaaaa aaaatcgatt ttgttatttt ataaaaggag cgagaaaatg acaccgattg
    23941 agctaaaaag ctacagaaag aagttaagtg caattcataa tataagtgga gataaaaagc
    24001 cgctatcaca agcaagattt gcgcaattga tgggctgctc aaaagattac attagaaaga
    24061 tggaacaagg gaaaagaaaa atcccagagg attttgaaca acgaatcaag gaacgctttc
    24121 gattgtatat gaaaagcgga atagatcttc gagcaatgat tgattatttg cgtctttcct
    24181 tttttgggac agactacaaa acagtgattc aacgtgtatt acgaatggat ctgacagaat
    24241 ttgaaacacg tccaacgggc ttgtatcttt ttgactgtct ttctgttcgt gggaatatct
    24301 gggtgttctg gcattcaaaa cgaactgtaa cagaaaatgt acttgtgcaa ttttcaggac
    24361 aaggttgccg ggaatatgag gaagtcatgc gtgaacaaca aacagattgg cgaacatggc
    24421 tatattccat ttggaaaaat caaggaaagt tggaagctgg ttatacacgt gtgcaatgta
    24481 gccgattcga tattgcgtta gatgaacttt ggggattgga aacggaagaa catttcgatc
    24541 tctttgaatt actagaaaaa caaaaagcag gtttattaga aatggacttt aagagattcc
    24601 agaatattgg cggttgtttt ttagatgatg gctattttcg aagtgaagga ttatctttgt
    24661 attttggatc gcgaaagagt cctctctttt ttaattttta tgagaaacgt tttgaaattg
    24721 cgaaccgaga aaaaatttca gaattagaag cattaacgaa atatggtatt tataatcgat
    24781 atgagttacg gttagccaat gagaaagcaa cacaagcagt agaagcattt attttaggtg
    24841 attcaccgaa acgtttagga gagattggcg ttggtttaat taatgcaaaa cttattgttt
    24901 gggatagact ggaaagtgat gcacggattg ttgataaacg atgggcaaaa ttatttcgtt
    24961 cgatggaaga attggatttt acagtaaagc cagaaaaatt ctcattagat cgttcagaac
    25021 gctggttttt gaaacaagta gctccgacat taaagttgtt acaagaagca gatcgtattt
    25081 taggaagaga ctttgtacaa gaagggattg ataatgccga actaaatgac gagcaacaaa
    25141 actatctaga atggatggga acgtcaggag ggaaaattgc atgaatgcat atacgacaac
    25201 agaatggctg cgcctgttgg atttaaaggc tgcaatcgaa gcattaaatg aaaagatggt
    25261 tgatttgtct tattttcgtt ttcgagttcc ttatattgaa caagcggtaa aagctggacg
    25321 ttatcaggaa aaagagaact ggcaagaaat tgcacgctta ttagaagttc gtaagggata
    25381 cgaacaagaa ctcgaagagt tggaatttag ccgaaggaaa ggaaggctag aattcattcg
    25441 cttttatcga ttttcattgc caatacctgc gattttagcg gttaaaaaag gctgcgataa
    25501 aatgaagata tatgaaaact gtgtggcagc acttagcagc gaaaaacctc ttatggaaga
    25561 aatttcattg gtgactgtca cttgggaaat gagtcgccag ccaacagaag aacagacata
    25621 tctttcttta gaagagattg agatagaatt agaggaaatt ggtcgctatg ccacttgttc
    25681 tacctattgt ggatcggtca ttagtattgc tggtgtgatt gtctaaatga aaaaaattat
    25741 gatttatgtt ggtgcgtatt ggtcacgtga tccaacagta ttagaaaatc cagaggcggt
    25801 acactattgc ttgcgccaat tattctatct gtataaagaa cgattagaat cgttgattcg
    25861 tcagttacca tatactgaca gacgtttaga tgaacttctt ctaaggtatc cagcaatgta
    25921 taaacgacga aaaaatagat tgttaccaga agagtatccc attgaaaaac atgaacttga
    25981 agggcgattt gtggcttatt tctatgatga tgttcgtatg cgcttagtag aacaacgaat
    26041 ggaaatagaa aatgatcgct actattttat caattattgt aagaagcgta agtatcaagt
    26101 aactgatgat ttttatgatt gtattttaca agatggggaa gtgattctat ccaaaattgc
    26161 tccaagtttt cgtgaacttg tgccgattga ttttttgaaa aaatgtcatt tgcggattct
    26221 tccgtaaaaa agagctataa aaggaaagaa aagtagaagc caaatcggtt tctgcttttt
    26281 tatttttttt aggaaggaga agatggtatg tcactggaat ttttaggaga gccaattgaa
    26341 aaggaagagt tcgtttcgca ttatatggat ttgtttattc aggtgataaa aaactttgtg
    26401 cgattgatga attttatcca cgcgaacaag actatctagc cgctgaacga ataaaaatca
    26461 gtttattatg ggaaaaattc acagatgaaa ataaccgacc gccagattat cgctatttat
    26521 cagattgttt aacgaataat gtgctttcta aagaatacct atttcacaaa cgtgagaaaa
    26581 atcaaatgtc ggttgaacat ttttgtgatt gttatattgc attattgcgt agtcaacagt
    26641 tattaacagt agaacgatat ggtgcagagt ttggttatgt cttgcaagct gagcgtcata
    26701 gagctacaag atgctatgaa aaagaagatc aagtgattga tggctatgaa gaattatgta
    26761 ttcgaaacaa tcgctatttg caacaacgtt tatatgaaca attgatgatg gatttttcaa
    26821 atctctatag agggtatgaa tgttcgatga ttcaagaaag gtagaagaaa agggggaatt
    26881 tttatgtggc gattattatt tttggtggta tgtggtatgg cattgttcta tgtgtatcgt
    26941 gtctatctct atccacgaat ggaacgatgg acaagttggt atagagatcc tttaaaacaa
    27001 cgaaaaggat tgaagcggta tcgttatgaa ttacttgttg aattttcgga tgggcgacaa
    27061 gcacagtatt atttttttgc aaactatcca aaatacaaca ccagtgaact attgattcaa
    27121 ttattcaaaa ataatccata tgttgggtta gaagaaaacg gacgggagtt cttttatcgt
    27181 tcagataata ttagtgcggc gtctatgaac aaaacacgaa ttgcataaaa aattggagga
    27241 ataatcatga aaaaaacaac attagcagtc gtaacaacac taggaatttc attattttcg
    27301 tttcaattag ggagttcatt ggtttatgca gatgaaactg tagaaacacc taagtcagaa
    27361 attgtagaaa cattgccagg ggaaggatta ccagatgttg agacagagct aaatggagga
    27421 acaacggaaa aaccagcaga acctgaacaa ttgcagtcag agaaacctga aaaagagcca
    27481 gtgacaccag aaccagagtt tcctgaatca actttaccga ctgattcgat cgaagaagaa
    27541 ggtgtgcata gttcaacaac aacggattca agtatagtca caactccgga acaatcagga
    27601 acaaccagtt cttctgaaaa gaatactgat tcgtcaagta cggaacagcc aacgatgtcg
    27661 tctaccagtg aagaacaaaa agaagaaccg aaacaacctg ttgaaaaatc agaagcaatt
    27721 ccaaaagaaa ataaacctat tgttccagca aaagaagtca cagtatcggt aaccccaagc
    27781 ggtgaaatta cgacgaatac ttctcaagga atgagtgtac cgattgtcac tagtaatgta
    27841 gaggaattga cccatattcc aacaccaacc accccattaa aagtagcagg tgatcaaaca
    27901 attgttggtg taaaagatgg tattccatta atccaagata gtgaagggaa tttggtaaag
    27961 gatttgtcga ttcctgtaaa gaaacttcca agtggcaata ttgaaatcaa aacagcagat
    28021 ggaaaaacaa aagtcttacc aaagactggt gaagaaatac atgttgcact ttctgttcta
    28081 ggaggagtgt tgacaagtat tgctggattt gttggatata aaagaaagag aaaaggaata
    28141 gaaaatgagg gtaattgaat ggactatttt tcagaaaaac aagcttatcg ttgtcaatcg
    28201 tacgtactag taaaaacata ctagaaggag tagcttatga aaaaacgaca actacggcgg
    28261 aaagaaaaag tttatacaga gaaagtaccg aagcaaaaga aaatccattc aaaaaaagca
    28321 gtgaaatgga tttggtgttt ggttttattt ctttgttttt cagggggctt ggcatttttt
    28381 caatccatga aaacgaaggt tcaagtgtcg tttttgaaag atgaaatcca acaattacaa
    28441 aagaaagaag tacaaagtcg gtcagaatta acgtacacac ctgaaattga atcctttatg
    28501 aatcgttttg ttgctttgta tatgaataca ttcgataatc atgaacaaga acaacaacgt
    28561 caagaaacat taaaaaaaga gttttatgca caaaacttta ttcaagaacc aggaagtgtt
    28621 tcaggtgttc gaaaattaac tgctgcttcg tttattggat taaaagaaat tgaaaccatt
    28681 ccaactgcta gttataaagt aacttatgat attcgctcga tgaatccaga gagtaagaaa
    28741 gaaacaagtg tgacaatcac acaagtactc aatcttccct tttctatttc aaacaagggc
    28801 tgtcgtatta ttgcttatcc ttattttacg gcactaccaa agaatacaga aaaacaacga
    28861 atgattactt atgatgacac attatacgaa acggttgatg cgtcggaagc gaaagatgtt
    28921 gaaggatttg tggaagattt cttagataag tatgcgaaaa acaatgcaaa agatatgtca
    28981 tacatgatga aagaacctga aggactcaat caaacagcta aagttgatca agtagagatg
    29041 aaagtattgc gttataaaga aacgaaaaaa ccttttattg taaaagcaac ggtcgtcttt
    29101 cgagatggtg atacgtcact tttacaaaaa gaacaaatga cgattgtcat tacgaaaaaa
    29161 acaaatcagt attatgttga aaaattaaca cataattggt tggataggaa aggagaagaa
    29221 cggaaatgac actgaaggga attgtagatt ttatattaac gcaaggtggc tattttttaa
    29281 taatggtggt tgtttatctg ggattgcgaa attttatgaa atcaaaagta ggaaatgcat
    29341 taatatctat cgcagcaggt gccgcagttt atttcttctt aaatgatcca acacgtgtat
    29401 ttgatgcgtt tggtaaaatc attgaaaaat tatttatgga ttaggaggat agtgaagtgg
    29461 attgggaacc ttatgattat tcgattgttt atgcggaacc ttatcgcgta caacagatta
    29521 caagcaattg gcgtcttcca tttccagttg cattagtcaa tgcagcaact tttttgttgg
    29581 tcgtaatctt tgaatattta tttttacgtt catttattgg ttgggtcatt tctttttttc
    29641 caccagcagg agttgttttt tatataggac tcccctattt atgtgtgtta gcactggcaa
    29701 aactaaaacc tgatggaaaa aaagtgcatt tttatttatg ggattggttt tgttatatga
    29761 ttcgtatgat gaatggtgat tgtgtgtatt ataactggga caaggtatca aacaatgcat
    29821 tggaagaatt actttttgtg aataagtatg tttatttaga ggattagaaa aagggacaat
    29881 caactggttg ttcctttttt attgggaggt gaaattcatt ggtgcttgta aaagaaccag
    29941 taaaagcatt caagaaaaat tgtttgttaa atggagagga ttgtatctgg gcctactatc
    30001 atgtttttcc acgaacaatt tcaaaaaaca caccaaaaaa gacacaggaa ataaaagaaa
    30061 tgatggctcg tttactgttg cttttagcag attatgaaga tgtggagttg gtgatgtatc
    30121 cacgaacaat gaatttacgg acgaagtttg acacattagg aaagggattt tctcccgata
    30181 gtcaagcaat tggtgcgtat tatgcaaaag aaacagtgac attgttggaa cgagaaggtc
    30241 aagtctatcg tccttcattt attttaggaa tcaaagtcaa agaaaaagca acggtggaga
    30301 gttttcatga tgtaataaat ctaatctggt tagatttagc aggaagttta gcacgcttat
    30361 ttggattaga agtagatgat ttggaaactt tgttttctga aagtgatcct tatgtaaaag
    30421 aagtgacaca attactttat agcttttcta ttcgtccctt agtaaaaaaa gagctgttgt
    30481 atctcaatcg tctgcaatat attcgaggaa tggctcacaa agtagaagaa caaaataatt
    30541 gggaagtact gaaagacaat caaggaacgt atgtacgtat accgaaggaa cataaaggcc
    30601 ttcttcaatt agaagatgag ttagggagta gttatttagc attgttgcct attggggaaa
    30661 cacctgaaaa tatgagaaat tgtgatctat ttcaatttgc tcaggatctt tctttcccag
    30721 cagaattgcg aatgaaaatt cactatccac gcttgaaagg acagggaggt attggtttaa
    30781 aactgaattg gctatcggaa cggttcaaaa cagagcataa ggaattggtt caaaatgatg
    30841 aagaacctag tgaacgctta ttaactgttc gaggattagt aaaacattta aagcaacgag
    30901 tagataatgg gcaacctgtc gtcgattgga tcgcatgttt tattgtatac ggtcaaacgg
    30961 ttgaagaagt tcgtcaaaat agtgcaacgg ttattcaaca acttcatacg attatccata
    31021 tccaccgtgc aaaaaatgat caacgagaat tgttctataa atgtttatta gggcaaccat
    31081 tgggacggca gttacattgg aagcaccgaa caactgtaga agcagttggc gaatggttgt
    31141 tttcagcagt atcagaatta ggtatggaat ccggctggta tattggtcgg caagatacat
    31201 taggggatgg tggttctcga ggagaaaaaa caccattaga agaattaatt tatgcgtcaa
    31261 atcgttttgt ctttttgaat cttctagcca ttgcggaagg aatcaaaggt gcgttgtttg
    31321 atgcgcctca tgtagcagta actggaaaaa ccggtaaagg gaagaccttt ttagttggct
    31381 tgctcttttt gtatagttcg tttatgaatg tacaaagttt atttgtggat cccaaaggag
    31441 aaaagcgcaa atggtttacg aaactcattc aagacccgta ttatcagaaa cattaccctt
    31501 tgtttgtaga acatttgcaa aaaatgagtt atgtcacgtt agatgctaca aatccccgta
    31561 attatggtgt attagatcca attgtttgtt tatcccgagt agatgcgaaa caagtagccc
    31621 aggatatgat tgatgagtta agtccgttag gtaccaatca ccttttaaaa ggggcggtat
    31681 taaagggaat ccaagaagtt ttagttcaaa aagaagccgg acaacaagtt ggcttgatgc
    31741 atgtggtcga ctatttagaa caaatggaac aaaaagaggt tcgagaatat ggtgagtttt
    31801 tacgcttgac ggtagaagat tcgattttac gtttaggatt ttcttatgga gaaaacccgg
    31861 gattagactt taatgccaaa acaacgattt tagagatcca agatttgaaa ttaccaaatg
    31921 acaatgtccg accagaacta tatacagatg cggatcgaaa gtctttgtgt ttgatgatta
    31981 gtttaggtcg tttttgtgaa atgtttggaa aacgagatag tagtaagaaa acagcgattt
    32041 attttacaga agcatgggta tttaataatt cgaacgcagg acgtagtatt attgcagcaa
    32101 tggcacgtgt ggggcgttcc cagatgaatc aattagtgtt agatacacaa tttattggtg
    32161 acttaggttc agataaagaa aaagggaatt ttggtgtcgt ctttgctttt gatgaagaca
    32221 gtgagcgaga aaagattttg aatcatttac gtttggaagt aaatgaagcg aacttagctg
    32281 aaatgagcca catgattaaa ggacagtgtt ggatgttaga tccctatgga cgtgtaggaa
    32341 aactcaatgt acacagtttg tttgaagaaa tgaccgctgc attacaaaca acggaaaaaa
    32401 caagtcactc tcaagtagaa gaacaggata cgttaatcat gcaatagaaa tgaggtgaaa
    32461 caatggctta tataaagcat aaggaaaaga aatggtgcgt tttgtttttt ttaacgattg
    32521 gattcggact gctttttgtt ggtggattgg tgtatgcggc agaaaagcag gtggatcaat
    32581 cgatctatga gattgggaaa tttcatattg ataccttttc ttcttattta tctgatagtg
    32641 aattttcatt aggcaaaatg ttatttgggg caaatggcac agattttcta aaaatgatga
    32701 cgaatatgtt ctttagttta agtaaattga tttggcaagg ccttgatttt gtcattgaga
    32761 agctctatga aggagctgca atgaacgagt taattcacca gtttttcact ttttcggata
    32821 ctatttattc aaaaatatac gatcctattg gtgcggggat tgtcgtcttg tatgtgttat
    32881 atctatttac tcttcgtata tttagaggga aacattacgc aaaaatggtg tttattcgat
    32941 ttctcttcgt tgtcattttt tcaatggttt ggtttggcta tggtagtaac gtacctagta
    33001 aaggagaagc ttttaccaaa gggttaaatg atttgtccgt ggaggtagaa ggctttattt
    33061 tttctgctac aaatagtcta gatgaattgg cgacaaaaga tgtcaatggt gttttagtgc
    33121 caacgacaca aaaggaagcc attcaaaaag tgcgccaact ttattataaa gcggctgttg
    33181 ttgatccgta tatattgtta aattatggga caacaaattt agaagaattg tatcaagcag
    33241 agattgaacc acaagaattt ctagccaagg aagcgtcaag tgaagaatta tctgatattg
    33301 agacaaaagt gaccactgct gcaggagaag atagagggaa aaagaaggct tatcgtgtct
    33361 atattcaacc aactaaagct gtttataaag cagtcgttgg ttctatggtg ccattgctta
    33421 atctaagtat tggattacca cttgtagtga ttggcatgat tcgttttatc tttcaaatgt
    33481 tggtgttgtg tttattactt gtgattcctt tttcattact cgcttctttt gttccagtat
    33541 ttgattatat gctcatgaat accttaaagt cctttttggg cttgatgttt caaaaatctt
    33601 tttacagtgt gattattttg gtggcatttt tgatctataa catagtagac gatttaattc
    33661 caatgaatac tgtttttggt tttttaggaa atatgtttgt caaagcactc ctatcaattt
    33721 tactcattgt aaaacggaaa gatttattaa agaaactagg gttaggagct gcagatcaaa
    33781 ctataaacaa cacgaaaaac atagcaagtc attctttgtc tcaaatgaaa caactgcctg
    33841 gtcaactatc aactgctgga caaaatttgg caatcaaagg tgcaggatta gcagggaatg
    33901 tgtatcgagc aacgaatcca ctcgcttctg cagcgataca tggatttcaa aagtttcaac
    33961 aaaaacgtac accacaagaa tcggtaaaag ggaggcaagt ggttaatccc tttgaactag
    34021 gtgggcgttc aaatcaagcc ttgccttata gtggtcgtca tgttcgttat catggggtag
    34081 agcctgaaat gtcttctacc aaatcgaaaa cagagtggaa ttctggtctt tcaaatggtt
    34141 tgattccaaa ttcgtccatg aattcagtaa gaccaaaaca acaggtacaa tcattggcac
    34201 aattgagtaa gcaacaaggg aataatcaaa gaaaaccatt gttaaataaa ggtatcgctt
    34261 ttgttggtca atcgattccg ttgccttctg gaaaagtacc cgtgccacaa ttaacagctg
    34321 ctggttctct ttcacaacag attcgccaag tgaaaaatca ggcgaaaaat gggagttatc
    34381 cagcaatgca gcagttattg aaccaatact ctgttcctaa ttcgtcggta gccactctat
    34441 caggaaatcg ttctgtgcaa caaccggggc aacgttcgtc tgaaagtaaa ccgtcgattt
    34501 ccgcaccaaa atcgttacaa cctgcatttt cttctttaga taagccaaca gagcaatcgg
    34561 aaaaaataga aaaggatcct aaaacactgc cgattttgga cgcaaaacca ggaactgatt
    34621 ttactttacc aaaaggacga acagtccaaa aaccattatc gaagaaagga tgaggaagaa
    34681 atgaagaaag ggtgtctagg ttgtcttctt ttctttattc ttcccttttt tctattgcca
    34741 ttcttttttt caggagaact ttctgagcat ggaggagaag ctacaggtct agctgtcgct
    34801 tttaatgggg aatataccga aggattaccg attttttcag agattaaagg acgtggtcca
    34861 atcacagatg aacatgcgca agcagcagtt ggtgcagctg taaagtatca gttattaccg
    34921 agtgtgattc tttctcaatt aggctatgaa tcagcttatg gacagtcaca atctggaaga
    34981 aacgatacga acttttttgg gattacctgg tttcctggtt gtccctaccc acaaggttct
    35041 gcacgtggaa ttggtggttc tgaaggtggt ttctatatga agtttccgaa cttaaaagct
    35101 tgttatgact attatggctt tatggtggcg agccaaagca actttaatca atgtgtgggc
    35161 aataaagatc ctggaagttg tttattgatt ctaggtcgtg gaggctatgc ggcagcaggt
    35221 attaccgaag ggagtccgta ttatcgagga gcaatgagta tcataacgtc ctataactta
    35281 acggaatatg atacttttgc gatcaagcat tggaatagcc taccaacaaa aaaaacttcg
    35341 cttattgcag gaaaaagtaa aggcaatgcg aaggttttgg atcggatcat tggacgatcc
    35401 gtaaataatg gggaatgtta tggtggtacg gcatggtatg tatctcaatt aggtggccca
    35461 cagttaatgg gttctggaca catgtttgcg atgttaatag gggaagatta taactggaca
    35521 acctatggtt ggcaagtaat catgaaccca aaaccaagtg atttacaagc aggagatgtg
    35581 gtgaattggc aagcaggtgg tgcattgtct cctggaatct atggtcacac cggaattata
    35641 acagatgttt catatggcgg acaacatttc tctagtgtgg aacagaatgc cgagcaagga
    35701 cggattatgg ctcggtatca acgcacgtac aatcaaacaa aaattcgtag tctggttcgt
    35761 aaggtgaaat aagaaaggca gtgactagaa acgtgaaaaa agggataatt ttagtattta
    35821 gttggttatg tttgataggt cttactggtt gtcattggtt ttcttctgaa aaacaaaaaa
    35881 cacaattgag aacgacagaa gaaacaatcg aagcagtaaa agaaacaagt ggaactcttc
    35941 aaagtactga aagtaacagt caaattggtt cattagaaga cgagacagcg caacaaatgc
    36001 aaatagatga aatgacaaaa aatttcttaa aagattttgc tagaaaatgg agtaatttca
    36061 cagatgttta taaacgcaat caagctgtga gggactatat gacagatcgt tgtattaaag
    36121 acaatagtat tgatgtagac ccgcatgtac aaatgaaaac aactgggaaa ctttaccagg
    36181 tgacacaaga tatagagaat ccggatcaat atttgttatt tggagaagaa acaatgaata
    36241 aggttactca atttgtatta cttcaattga aagtagatca agaagctcaa aagattgatg
    36301 cgatcaagat ttattatgta cggagtgcgt attaaaataa aaaatgaaca ctcatattta
    36361 ttatacaccg aagaaataga aaaaaacaga ctgttttttt aagaaaaact atgtcattct
    36421 ttacttgaaa aaagttgtta taggacagaa aggataaaac aaaaacggaa aaaatatgtc
    36481 ttatgaataa ctataagaac tattctattt tttaggagat ggatacgaga tgaaaaaact
    36541 aattggctat agtgtgatgt gtattggatt tgttggtgtg ggattgtttg tatcgactga
    36601 atctgcttct gcggctgaat ggtataatcc tatctggtat aatccgaaca ttgaatgtca
    36661 aaaaggctat ccgtatggac cgacaaatct gaaaaatgga aaatgtagag caaattggaa
    36721 tggcgttgta gatagtgtaa ttactggttc aatggactct gctctacatg gctttacggg
    36781 tcactagaag gaggaagaaa atgtctgaag agaaaaaaga aatcgttgaa tcattagtag
    36841 ctttaaaaga ttctctttct aagattgaat atgttgatag gcaagaaatt caaaaactaa
    36901 ttgatgatac tattattgaa atccaagatg cgcgttgtga aggtataaaa atttctgtag
    36961 ctttatctaa agtaatagaa aaaatgaacc gttcattagc gtttaatgga ttgaagctag
    37021 atagacaaac tagtctgata tgggatcatt taaaagactt gtatgacaag tctaaaagaa
    37081 gtgaacggac tgctgtatca atcttaaaag gcctttgggg aatgaattca taaacaagat
    37141 atttaaaaag taacttattt tttatggagg agaaattatg aaaagaataa ttaaaatatt
    37201 tggtgtttta ggtgttttct tattaggttt aagtactttt gttagtactg gaacaagtgt
    37261 gtatgccaca gaaaaagtga atacagttga tttagaaata aagaataaat tagtagatga
    37321 attaactttt tattttgata cagttggttt tttagacgaa catggtgtat accatataaa
    37381 ggattttggt gctattcgtg aacgaatcag tcaagataac gaacaaggaa gagcagcaag
    37441 agtcttttat gaacatatta ttgttcctag aatggagaaa gttgattgga aacgatatgc
    37501 tggatgtgta gccattaatt ctcttcctgg aggaggagtg gcttgggcta taggtacgca
    37561 gggagatttt attgaagcat taaaagcttt taattttgaa aaagctgcgg atttattatt
    37621 aaaaattgca gaaaaacaat taggaaaagc tgcagctaaa gagttagcta aggtaaatat
    37681 tgtagcagga attgcagcag ctgctgctag ctgtgcatta gagtaaaagt ttatgaaaag
    37741 tttagaacaa gagcatatgg cttttaaaca agcaatgttt aaagaaaata tctatttaaa
    37801 ccataactat attcgtgttt ctaaggcatg ttcaccagta ttgaatatgc ttggaggtgg
    37861 aaatggatta tatcatttac tgtttgtcga tgtttgttgg ttaatttttt taccagatga
    37921 actagttata gtcaatgaaa aaataacaag taaaaatgaa gtgtttaatt attctttaac
    37981 aagaattaat tacaaagaga ttaccaagtt ttcactagaa aaagttcctt tttggggaga
    38041 attttgttta aaaattaaat gtaactggaa aagaatgtat ttctatattg atggtgatga
    38101 tgctcttaca ttcggtaaaa ctactttttc tagttttaat tttcagtttc tattaaaaaa
    38161 taatttttat ggtttattga aataaggttg cgaaggaaga tgtagagtta tttgcactgt
    38221 tgtgttggta tatctcttaa tgctgtgtct ttttggggag attaattagt ggagtgatta
    38281 tatgagtata gatgattgtt tagaagaaca aataagatta tttttaaagt ttttaaaagt
    38341 taaagttcaa aataactact tcacatgtta tagttatgca caccctgttt taaatagtct
    38401 tccttttaat tctttagtgc aaggattgat tacaaatatt gttttattag tctgtacacc
    38461 aaatgaaata tttgtaaaaa agataggcag tggtatttct tcagtaagat taaaagagaa
    38521 agaatatact caaaagatta ttcatattcc taagcctgaa attaagcgtt ttgagataaa
    38581 agactattct gctttagggc tgttctcaat tggttactgg tgataaaaaa taagaatact
    38641 aaaactatac gtagaatata ag
//
LOCUS       DACDRN010000017        38476 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDRN010000017 DACDRN010000000
VERSION     DACDRN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.017"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(63..938)
                     /locus_tag="IWJ80_001139"
     CDS             complement(63..938)
                     /locus_tag="IWJ80_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9460884.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            complement(1047..2816)
                     /gene="aspS"
                     /locus_tag="IWJ80_001140"
     CDS             complement(1047..2816)
                     /gene="aspS"
                     /locus_tag="IWJ80_001140"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP9460885.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            complement(2831..4123)
                     /locus_tag="IWJ80_001141"
     CDS             complement(2831..4123)
                     /locus_tag="IWJ80_001141"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP9460886.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4517..5464
                     /locus_tag="IWJ80_001142"
     CDS             4517..5464
                     /locus_tag="IWJ80_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9460887.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(5553..6812)
                     /locus_tag="IWJ80_001143"
     CDS             complement(5553..6812)
                     /locus_tag="IWJ80_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP9460888.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(6983..7429)
                     /locus_tag="IWJ80_001144"
     CDS             complement(6983..7429)
                     /locus_tag="IWJ80_001144"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP9460889.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(7440..9653)
                     /locus_tag="IWJ80_001145"
     CDS             complement(7440..9653)
                     /locus_tag="IWJ80_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP9460890.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(9886..10650)
                     /locus_tag="IWJ80_001146"
     CDS             complement(9886..10650)
                     /locus_tag="IWJ80_001146"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP9460891.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(10651..11598)
                     /gene="prmA"
                     /locus_tag="IWJ80_001147"
     CDS             complement(10651..11598)
                     /gene="prmA"
                     /locus_tag="IWJ80_001147"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP9460892.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(11611..12099)
                     /locus_tag="IWJ80_001148"
     CDS             complement(11611..12099)
                     /locus_tag="IWJ80_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP9460893.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(12065..12736)
                     /locus_tag="IWJ80_001149"
     CDS             complement(12065..12736)
                     /locus_tag="IWJ80_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP9460894.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            12874..14160
                     /locus_tag="IWJ80_001150"
     CDS             12874..14160
                     /locus_tag="IWJ80_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP9460895.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            14232..14426
                     /gene="ssrS"
                     /locus_tag="IWJ80_001151"
     ncRNA           14232..14426
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWJ80_001151"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            14504..14788
                     /locus_tag="IWJ80_001152"
     CDS             14504..14788
                     /locus_tag="IWJ80_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460896.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            14861..15340
                     /locus_tag="IWJ80_001153"
     CDS             14861..15340
                     /locus_tag="IWJ80_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9460897.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            15563..15706
                     /locus_tag="IWJ80_001154"
     CDS             15563..15706
                     /locus_tag="IWJ80_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP9460898.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            16284..17867
                     /locus_tag="IWJ80_001155"
     CDS             16284..17867
                     /locus_tag="IWJ80_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9460899.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(18304..19488)
                     /locus_tag="IWJ80_001156"
     CDS             complement(18304..19488)
                     /locus_tag="IWJ80_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP9460900.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19513..20520)
                     /locus_tag="IWJ80_001157"
     CDS             complement(19513..20520)
                     /locus_tag="IWJ80_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9460901.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(20692..21051)
                     /locus_tag="IWJ80_001158"
     CDS             complement(20692..21051)
                     /locus_tag="IWJ80_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460902.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(21023..21394)
                     /locus_tag="IWJ80_001159"
     CDS             complement(21023..21394)
                     /locus_tag="IWJ80_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP9460903.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(21411..21713)
                     /locus_tag="IWJ80_001160"
     CDS             complement(21411..21713)
                     /locus_tag="IWJ80_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460904.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(21730..22200)
                     /locus_tag="IWJ80_001161"
     CDS             complement(21730..22200)
                     /locus_tag="IWJ80_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP9460905.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(22175..22453)
                     /locus_tag="IWJ80_001162"
     CDS             complement(22175..22453)
                     /locus_tag="IWJ80_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP9460906.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(22450..23499)
                     /locus_tag="IWJ80_001163"
     CDS             complement(22450..23499)
                     /locus_tag="IWJ80_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP9460907.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(23456..24385)
                     /gene="cpaF"
                     /locus_tag="IWJ80_001164"
     CDS             complement(23456..24385)
                     /gene="cpaF"
                     /locus_tag="IWJ80_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP9460908.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            24517..26271
                     /gene="ade"
                     /locus_tag="IWJ80_001165"
     CDS             24517..26271
                     /gene="ade"
                     /locus_tag="IWJ80_001165"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP9460909.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(26369..27031)
                     /gene="trmB"
                     /locus_tag="IWJ80_001166"
     CDS             complement(26369..27031)
                     /gene="trmB"
                     /locus_tag="IWJ80_001166"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP9460910.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(27101..27880)
                     /locus_tag="IWJ80_001167"
     CDS             complement(27101..27880)
                     /locus_tag="IWJ80_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP9460911.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(28087..29298)
                     /locus_tag="IWJ80_001168"
     CDS             complement(28087..29298)
                     /locus_tag="IWJ80_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9460912.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(29291..30028)
                     /locus_tag="IWJ80_001169"
     CDS             complement(29291..30028)
                     /locus_tag="IWJ80_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9460913.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            30166..30600
                     /locus_tag="IWJ80_001170"
     CDS             30166..30600
                     /locus_tag="IWJ80_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP9460914.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            30593..30937
                     /locus_tag="IWJ80_001171"
     CDS             30593..30937
                     /locus_tag="IWJ80_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9460915.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            31117..32127
                     /locus_tag="IWJ80_001172"
     CDS             31117..32127
                     /locus_tag="IWJ80_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9460916.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(32196..33140)
                     /locus_tag="IWJ80_001173"
     CDS             complement(32196..33140)
                     /locus_tag="IWJ80_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9460917.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(33124..35844)
                     /locus_tag="IWJ80_001174"
     CDS             complement(33124..35844)
                     /locus_tag="IWJ80_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9460918.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(35841..37061)
                     /locus_tag="IWJ80_001175"
     CDS             complement(35841..37061)
                     /locus_tag="IWJ80_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP9460919.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(37342..38187)
                     /locus_tag="IWJ80_001176"
     CDS             complement(37342..38187)
                     /locus_tag="IWJ80_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9460920.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT        11383 a         7963 c         6717 g        12413 t
ORIGIN      
        1 aaactgcaga caaacgaacg tctgtctaca gcagtatttc atgaattttt gtcaaactcg
       61 atctaaatgc catcctcata gaaacgacca agtatctgga cgttttctgc aatacttaca
      121 aaagcttgtg gatcagaagc tttcattgct tctctaagta gaggcagttc gtatcgtgta
      181 acgatcgtca tcaatactgt ctgcttatca tgcttataag caccttctgc ttcattgatg
      241 atcgtgatcc ctctacgcat acagttttgg atttcattta ctactttttc tggatgtttc
      301 gttacgatca ttacctgcat cttcttttgt ttagtataaa cgacatcagt tacttttcca
      361 ctgacgaaaa tagacaacgt actgtaaaac atatattgcc agccaaatag aattccggca
      421 acaaaaacga tcaatgcatt gaagtagatc gaaatagaac caatcgactt tcctgttttt
      481 ttacgaatcg tgatactcac gatatctaga ccgcccgatg ataggccgtt tctcaacgct
      541 agcccaatac ctagtcccat cacagccccc ccaaacaacg cacagatgat tggatcggta
      601 gataggactg tttccggtaa aatatggata caaagggagg tcaaagtgac tgtgataaaa
      661 gtgaaaattg taaacttctt cccgattttt aaccacgcca atacaaacaa aggtatattc
      721 agtaaataca aagttgttga taccggtact tcgaatccta tcagtgattt tgacaaagtc
      781 gtcaaaatct gagctagacc agtaatacca cttgaataaa tatgtcctgg ctgataaaag
      841 aaattcaatg cgactgatgc gatcaacgca taaacgatag ataccgaaag cttggtcgtg
      901 taatcatgat aagcccaatt ttcgaatgtt tttttcatgg aaaagccccg ctttatatgt
      961 tatggcttca ttataaagtg tttttcaatt aaatcaaggt tgtcaaaaga agcgtattcg
     1021 acaaatcctt tttacctttt tatcggctat tcttctatat tcgttacatc aatattcaat
     1081 tcgaacaatt gtaaaggcga aaccatactt ggtgcatcac tcatcacatc tgctgctttt
     1141 ccgtttttag gaaaggcaat gacttcacga atattatctt ctccggctaa taacattgca
     1201 aaacggtcca atcctaatgc aataccgcca tgtggaggga atccgtaatc taaagctgtt
     1261 aacaagaaac caaattgttc ttccatttgt tcttttgtaa agcctagagt ttctagtact
     1321 ttttcttgaa gctcacgagt atgaatacgg atagaacctc cgcctagttc ataaccgttc
     1381 aagacgatat cataagcttc tgcatagact tttgcagggt cttcagccaa taacgggata
     1441 tcttcttctt ttggcatcgt gaatggatgg tgagcagaga catatcgacc ttcttcttct
     1501 gaaaattcaa attgtggcca atcagttacc cataagaaat tgaatttcga ttcatcaatt
     1561 agtcctaatt cttttcctaa acgtgtccgg atagcaccta atgcagcagc gacgatttca
     1621 cttttatctg cgccaaacat cagcaagtca cctggttttg catctgttgc tttaatgatt
     1681 gcttctgttg cttctcccat gaatttagca attggacctt tcaatccgtc ttcttctact
     1741 ttcagccatg caagcccctt agctccgaat tgtcctacat attgacctaa ctgatccatg
     1801 tcttttcgtg agtagcgatc tgcagcgcct ttagcattca atgctttcac gacaccgccg
     1861 ttttcaagag ccatttggaa gactttgaat tctacttctt tgactgtatc gcttagatcg
     1921 atcaattcca tatcaaaacg tgtatccggt ttgtcgctgc cgtagcgagc cattgcttca
     1981 tcataggtca tacgaggaaa tggcaatgtg acttcgatac cgcgaacttc tttcattacc
     2041 ttcgcaatca atccctctgt gtaagtttgg atttcttctg ctgttaagaa cgttgtttca
     2101 atatctactt gcgtaaattc aggctgacgg tctccacgta aatcttcatc gcggaaacaa
     2161 cgaacgattt gataataacg gtcgaatcca gcattcatca gcaattgttt aaacaattga
     2221 ggagattgcg gcaacgcata gaaatgtcca gcatggacac gtgaaggaac taggtagtct
     2281 cttgcgcctt ctggtgtaga tttccctagg taaggtgttt cgatatcgag aaagtcatta
     2341 tcatctaaat aatgacgaat cgcttttgtt gtcgcatttc ttaatttgat gtttttagtc
     2401 atttttggac gacgcagatc caaataacga tatttcaaac gtatttcgtc actgattgct
     2461 tgatcatctt caatttgaaa tggcggggtt tttgcttgat tcaaaatcac gatttcactt
     2521 gcaatcactt cgaattcacc agtggccatt ttaggattta ctgcattttc tccgcgtctt
     2581 ctaacttccc cagtcacttc gatcacgtat tcactgcgac atttatccgc gatttcccat
     2641 gcttctttgt tgatttcagg attaaagacg atttgaacga ttccttcacg atcacgtagg
     2701 tcgataaaga ttactccccc aaggtctcgt cttttttgta cccatccttt taatgtaact
     2761 gtttgttcta attgttgttc ggaaactaat ccgcaataga tcgttctttg tgccatttat
     2821 ttttttcctc ctactcgaat aaagtcattt catcatagac ttcatcaaaa tgttcatata
     2881 tctcttctaa tggaaaactt ttttcttttc ggcttgccat tgctttgaca ttaatcgttt
     2941 gactagcaag ctcatcttca ccgatcacca atactaattt ggcattcgct ttatctgctg
     3001 tcttaaattg cgcttttgct ttacggttca tgaagtcgcg gtcagctgaa aaaccaaagt
     3061 ttcgaatcgc ttggaccact ttcaatgctt cgatatttgt ttcgtctccg agtgcgacca
     3121 cgtaagcatc caactcatta aatgcaggga taactacttc ctctgcctcc atagtcaaga
     3181 gtaatcgctc gatacccatt gcaaatccga agccaggtgt tgaaggacca cctagttctt
     3241 ccactagatt gtcataacgt ccgcctgcgc aaatcgttgc ttgtgccccc atttttggtg
     3301 cgtcactcat gatttcaaaa atcgtatgtg tgtaataatc tagtccgcga accatattac
     3361 tgtcgatttc ataaggaaca gccaatgcat ccagcatgga aacaacggta tcaaaatgtt
     3421 tttgagcagg ctcgcttaga taatctaaga tagatggagc ttctgccaca aatttttgat
     3481 ctcgtttgtc tttactgtcc aatacgcgta atggattttc atgaaggcgg cgtttagagt
     3541 cttcactcag ctctgattca aaaggtaaaa gatagtcgat caatgcttga cgataggctt
     3601 gacgcgtttc tttatcacct aatgaattga tgactagccg gatttgcgtg atccccaact
     3661 gtttgaagaa atcaagtgcc atcaccatgc tctccacatc agttgcaggg ttttctgatc
     3721 caaatgcttc tactccgatt tgatggaatt gacgtaaccg tcctttttga gggcgttcgt
     3781 agcgaaacat tggtcccata taaaaagttt tgaacggttt cgcatactct gggccgaaca
     3841 atttatgctc aacaaaagaa cgaacgattg gtgctgtacc ttctggtcga agcgtcacat
     3901 ggcgttcccc tttgtcataa aaatcataca tctcttttga aacgatatcc gttgtatccc
     3961 ctacacttcg ggatatcact tcatagtgtt caaaaatcgg tgtacgcatt tcattatact
     4021 gataatctct gaataataaa cgtgctgttt cttcgacaaa ctgccatttt tctgattcgc
     4081 ctggcaaaat atcatttgtt ccttttggtc tttggaaact catagttttc tctccctttc
     4141 ttagctatgt aaataaaaaa atcacccttg ttcatcatga acaagggtga atgaaattca
     4201 tacacggtac cacctaattt tgaaagattt ctctttcctc ttacgaataa cgctcgtacg
     4261 cgggacggct tttcactcgt ccatctccag agtgtcttta ggctaatgaa actttagatc
     4321 attttcagcc tcgatgatcc tctcttgtaa agtttcaaaa acttactcgc tctttcattg
     4381 acattcgata ttacttataa cctactaaaa accagtctca aagtcaagag atatctgact
     4441 atttcccctc acaaaaacgt tttagttcag ctttttggta ttttgtttta gactagacaa
     4501 aaaggaggaa tcagctatgg cagaaataag aatcggacat tcacaagtct acgcagaaca
     4561 gcttggatta ggagcaaatg ccgttggagg acataatctt tttgacggtt tggaagatga
     4621 gacaggaaaa caagtagttc gtacagcttt gaatagcggg atcaatttga tcgataccgc
     4681 ttatgcttac ggaaacggcc gttccgaaga actgattggc gaggtactga aggaaaaaga
     4741 atatgatcgc tcacgtgtcg tcatcgctac aaaagctgct catgtaccaa ataaagggcg
     4801 aacatttgat aattctcctg agtttctcaa acaatcggtg gaagatgcac taaaacgtct
     4861 gcaaactgat tacatcgaca ttttttatat ccactttccg gatgaaagca caccaaaaaa
     4921 tgaatcagtt gctacattgc acgaattaaa agaagcagga aaaatccgag cagtcggtgt
     4981 gtcaaacttc acgttagaac aattgaaaga agcgaatgca gatggatatg tcgatgtagt
     5041 agaagataaa tacagtttga tccatcggca ggctgaaaaa gaattattcc cttatcttga
     5101 aaaaaacaaa atcagttttg ttccctattt tcctcttgct tccggtttgc tgacaggaaa
     5161 atatgaactt ggtgaggaaa aacaatttgg tgaaggagat cctcgaaaaa gaaatccaga
     5221 tttccaagga gaacgtttca gagaaatttt aaccgctgtc gatgtacttc gccccattgc
     5281 taaaagatac caagccactc ctgcgcaact tgttttagct tggtacatga agaatccacg
     5341 agtgtctgta gttatacctg gagctaagcg tccagagcaa gtatctgaca atgtccaagc
     5401 tttagatttg catttatcca atgaagatta tcaaaccatt gatgaagcat ttcgtggatt
     5461 ttgatcaatt gaccttatca atttatagta atatgaaaaa aggaagatca gcactgtttg
     5521 atcttccttt ttttcgataa gtttatgcta atctaattct tgtaaagtag cgctcggcta
     5581 ttcggataag tcggaaactg attgaatgga atcgtccaat cttgttcagg tacagagaac
     5641 tcaaacttat ttactagctg atcggaaaac acacgtagac tatgaagagt gatccattct
     5701 cctgcacagc gatgcatttg tcgaaaattt cccccgcctt gtgcaatcat ctcatattct
     5761 tctttatagg aaatatcttt tgctttccct acataccgtt tgatcataaa ggaatctggc
     5821 gcttcaactg ttcgttcatc atggtctgtt ccatataaat ctaaaataac ccaactgcct
     5881 tctggtatac gataaccatc tacttctact tctttcagcg aaatcgcagg tagcattggg
     5941 aagaaaggat aatagcgtct catttcttga ataaacggat cttgtaacgt cgaaaagtca
     6001 ttttttaatt gctgatacaa atctggtcta ctgaaaagtg catgtcccat caatgccgcc
     6061 cacacagtta gagcaaccgt tggacggatg atgttcaata attcaacagc tgccacttcc
     6121 agaggtaaaa gctgaccatc taaatctgtg gcattggcaa aagcatataa tgccacattt
     6181 tcttttccag ccacaggatt ttgtcgagct tcttcaatca agccttgcgc ccatttttct
     6241 gattttttgc gattttcgac accttttatg tgatcaatcg gagaagtaaa cgttccgctg
     6301 atcatagaga tttgatattc tgctaatttt tcgacctcat ctttagaaat cgctgataaa
     6361 ttgatacctg cccactcgca aatagatgta aagaaaacca tcttacttaa atcaaatagt
     6421 tcaaattgac catgctgtgc ttcaagtgct tgtgtcagat tcttatctaa gatacgatga
     6481 taatcttcca ttctttccgg tgtcattaga tccataaaaa tatttttccg atggtgatga
     6541 gcagctccat ccaaagtctg tactccacct tgaccaaaaa gtgtcttcaa tactaatttt
     6601 ggcatagcac cttttcgttt gaaattcctt ggatcataaa atacttttgc tgctgatgat
     6661 ccgtagatcg tgatagcttc tttattgaaa atctttgctt tgacgacggg agcgtccgct
     6721 tcatggcgta actcctccaa catgttgtat cctttttgat acaatttttt taaatctgtg
     6781 attttgatat ctactactgg tacttctttc atcttcagca acctcctata aaaaataact
     6841 tgtataattt aagcttaact ttttttccga ctgctgacaa aagaagcgct ttttgcagac
     6901 aaaaaagcca cttaagttag agtttatcag tctaacttaa gcgacgtgtt tcatagtttt
     6961 tactagtgaa aaccagatta ctttatttgt ttttagaatc aataatgatc gttacaggtc
     7021 catcattgac gagagaaacc gacatatctg ctccgaattg tccggtagcg actgtgatgc
     7081 cgcgacttct gattccttca ttgaatgatt cgtataaagg aatagctgtt tctggtcgtg
     7141 cggcttccac aaagcttggt cgatttcctt ttttcgtatc cgcaaacagt gtgaattgag
     7201 agatacttaa aatttgtcct cccacatctt ctacactaat attcattttt ccttgttcat
     7261 cttcaaaaat acgcatttgt gcaatttttt ttaccagata gtccacatct ttttgcgtat
     7321 cggtttcatg aatgcctaat aaaacaagta ggccgtagcc aatttttcca atttcttgct
     7381 gatcgactga cacagcagct ttacttaccc gttgaacaac tgctctcata tctgtcctcc
     7441 tatccattcg tccggcgtac actgtagaca tcaggtactg ctttgatttt atccacgata
     7501 ctctttaggt gggataaatt ctggatacca accgtcaaat ggatcgtcgc cattttatct
     7561 ttattcgtcc gtgcttcaac gctgttgaga tttttcgtta gcgcattgac tgtttgaagt
     7621 acatcattca ataagcctga acgattatag ccgtaaattt ccaaatctgc atcatactct
     7681 tttcgtgtat tcgatgtatc ttcccattcg acttcaatca aacgttctgc tacatttggt
     7741 ttgtcaggct gaacattcgg acaatctcga cgatgaatgg atatccctct gcctttagtg
     7801 atataaccaa cgatatcatc accaggaatc ggattacagc agcgactgat acgaattagt
     7861 aagttttcta ccccttgaat gacaacgcca ccttcatgac gtactttcat tttgtcaggt
     7921 tcttttttcg gctgattcat gatttcttct gcttcttgct tttgctgctc gattttttgt
     7981 tctttacgtt ctttttcagt cagacgattg gccatcgtca aggggctaac ttctccatac
     8041 cctacagctg catagagatc atcttctgtt tgataattaa aacgatcgag tgcttcttgc
     8101 agtttgttct tcgtcaaaat atctttaggc gtaaatccta gatctgtaat acatttgacc
     8161 acggattcgt ggcctttgat cacattttct tctcgatctt gggctttgaa gaaacgtttg
     8221 atcttatttc ttgctttgct agtagcaact aatttcaacc agtcgcgact tgggccaaat
     8281 gaatttggag aagtcatgat ctcaatgata tctccgtttt tcaatttgta atcaagctgc
     8341 accattttgc catttacttt tgcaccagtg gttttgttac cgatatctgt atggatactg
     8401 tatgcaaagt ccaatggtcc ggatcctttt ggtaactcag taacgtctcc ttttggtgtg
     8461 aagacgtaga ctttatcact aaagatatct ccttttacgc cttccataaa ttcagaagca
     8521 tcatagcttt cgtcttgaag ttcgagtatc tcatggaacc agcttaattg tttcgtcata
     8581 ccatctggtt ctactttttc attttttcct tctttatagg cccagtgtgc agcaaccccg
     8641 aattcagcga tttcatgcat ttcctgtgtt cggatttgaa tttctaccgg atttcctgcc
     8701 ggaccgatca cggtagtatg caaagattgg tacatattag cttttggcat ggcaatataa
     8761 tccttaaatc tgccgggcat tggtttccat tttgtgtgga ttgctcctaa tacagcataa
     8821 caatccttga ttgaatctac gatcactcga atagctagca agtcataaat ttcgttgaac
     8881 tgctttttct gatctttcat tttacgatag attgaataaa tgtgttttgg ccgtccatag
     8941 atttctgcaa aaatccccag ctcttctgtc gctattcgaa tatcttcaac agtaccactt
     9001 acgtattttt cacgttcttc tctcttcgtc tgcatcaagt ggacgatacg gtaatactgt
     9061 tttggattta gataacgaag tgctgtatct tctaattccc atttgatccg gctgatccct
     9121 agacgatgtg caagaggcgc atagatttct aaagtttcct gagcgatcct tctttgctta
     9181 tcttctcgca ggtgcttcaa cgtacgcatg ttatgtagtc tatcagctaa tttgaccatg
     9241 ataactcgta aatcttgagc cattgctagc agcatctttc ggtggttttc tgctagctgc
     9301 tcttcgtggg atttatattt tattttccct aatttggtta cgccgtccac taacatagca
     9361 acgtcatcgc caaattcttc tctcagatct tctaaagtga tttctgtatc ttcaacaaca
     9421 tcatgtaaga agcctgtagc cactgtatgg ggatccattt ttaaatctgc taatatacct
     9481 gcgacttgga taggatgaat aatgtagggt tcgcctgatt ttctaaactg tccgtcatgt
     9541 gcagcagtcg cgtattcgca tgctttctca acaaatgcta catgttcggg acccatgtat
     9601 tgactaacga gctttatgac accgggtccc gttaaaattt cttcttttgt cacaggaatc
     9661 gatccccctt tcttttttcg gtaaaacggg aatcgggaca aatatctgtt tgataattgt
     9721 cccaaaatcc gtgcagttat acgttttttt acggaaatgt tcgcttttta aaagcttttt
     9781 caaacgattt ttcgtatcca ttgtataaat aattgtttct attatacaaa ttgttgacta
     9841 ttttttcaat tctttctgtc gatactcttt tattaatgaa gaacgttagc tgaaagaagg
     9901 attcattaat tcccattgat agctagcagc acttaataaa taaagaggtg ctgtttctgt
     9961 acgaaggatt cttggtccta atccacaaag tatccctttg tgttctacaa aataatcgac
    10021 ttcatcgcgg gataatccac cttctggtcc aaagacagct aagattcgac tatctggttt
    10081 tgtttcacta agaattttgg ctgcatttga tttttcgcct tgctttgctg attcctcata
    10141 agcaaccaag accacgtcat aatcatctag ccgctcaaat aaatcttgtt tcgtttccaa
    10201 taactctact gatggcacga tctgccgatg ggattgttcc gctgcctctt gagcaatctt
    10261 ttcaagccgt tgttgtcgtt tcatacgttt ttttgcatcc catttaacga cagaggctga
    10321 tgccgggaaa ccaagaaaag cttgcgctcc taattcagtg gatttctgaa cgatccaatc
    10381 cagcttttcc cctttaggat agccactcgc tatggtaatc gaacttggca gttccttttg
    10441 attttcttct tttttaattt ctttcagata aacagcctct tcagtaatct ccgtgatttc
    10501 tgcaacaatg gctatttgat cattgaatgc caaaaaaact tcatcctcgg gttccatcct
    10561 cataacccgt acaatgtggt gatagttttc cccttctaaa acaaaatagt cttttgattc
    10621 atatgattct tttaaaaaat accgctgcat ctattccacc tctggttttt tcaaaataat
    10681 tgcataccag tctttttgct ggaaaatttg atccacgata aacccttgtt ctaacatggc
    10741 atccagaacc atttgcttct tctcttcaat gattcctgaa acgatcagcg tgcctgtttg
    10801 cttcagtaaa cgccaagcat ctgggatcat caataagatg atatctgcta aaatgttcgc
    10861 aacgattaca tcactttcaa tctcgattcc tgtcaaaaga tcgttagctg atacatggac
    10921 atctttggct atttcattca tatccatatt ttctttcgcc gaacgaacgg ctacttcgtc
    10981 taaatcaaag gcatagacat cttttgcacc aagatatttt gctgcaatac ttagcacgcc
    11041 cgacccagtt cccacatcta gcaatgtttc accaccacgc aagactgttt ctagtgctgt
    11101 caatgtcaag gaagtcgtgg gatgagtgcc tgtgccaaat gccataccag gatctagtgt
    11161 gataattttt tctagaggat cttctgtctg gtagttttcc cagcttggcg caaccgtcag
    11221 cattcgcgta atacgcaccg gatgatagta tttcttccag gctgttgccc agttgctttc
    11281 ctctacttca ctaactgtta ctttattttt tccaatagct agaccaaact caggtaattg
    11341 tgtgatacga tctttgataa aaggcaagat ttccggtaag aaagtcgttt ccggaaaata
    11401 agccatgact agtgcgcctt ctttcagcga tgtaaaatca tttttatcta aaatctcacc
    11461 atattgatcc gactgaaaat gttcaacatc taaagaatct tcaatcgcga caccactcgc
    11521 ccctgcctcc atcaaaatat tagcaactgc ttcgactgct tcactttcag tttctacttt
    11581 tacttcattc catttcatat ctgtcactcc ttaataactt gggtacggta aataagtatc
    11641 tgcttcattt tgctgtttta cttctactaa ttctgctagt tgctgattat cccagtgaag
    11701 ctcgaataca gcttcgtcat cgtcttcatc caaaaaatca agcaaatcgc tttgcccttg
    11761 agctaatact tcatttagat attccactaa agaatcagct actgattttg ccaacccttt
    11821 ttttccttca taagggatta ctgctagata attgcgttca tcaaaaacag atttctgagg
    11881 attaaaaaga agtacgccgt cttcaaattc gataattggt tcttgcgaaa cgatcccttc
    11941 tgcatcatca atctcaaatt gtgctttatt ttctgcaaac aagcgaacaa cgatctcgat
    12001 cgtatgattt tttgtatccc aatctaaagc tacatcatag tctgtcactt gtttttcaat
    12061 tatttcatct aaatatgtta atagatcctt ctttttcatt ttctttcctc caaccccaat
    12121 ctttctcgat ttggtttttt ctttgtaaag agatataagg atttcccgac acaacaaaac
    12181 gcaacggctt ttctgtccat acacctttgt ttgggatacc gattctaggc aaagcattga
    12241 tcttttttgg tgtctttttt ttctcaaaaa ctaagcgtaa cgaactatct gctatagact
    12301 gaccataaag ttcttgagga attgctaatg cttccactaa ttttcccgga ccgttgctga
    12361 tatctggacc agtttttccg ccacgatttt tactcatttg atcaatcatt gcggcgggtt
    12421 cgattgcacg tatcattact ccttgaggaa tcccttctgg ctgagtaatg atgttcaaaa
    12481 tccgatgagt atgcatcgtg taaaggtaga tcgtacctgg tttttcatac attgcccgca
    12541 cacgcggtgt tcttcttaaa ccatagctat gggcggcctc gtcttctgga cctaggtatg
    12601 cttccgcgtc tacaatatat ccagctaatc ttccttctgg tgtgtcatgc tccaaataca
    12661 tccccaataa atcccgtgcc acttctggcg tcgttttatt ccaaaaaaat tcatctatct
    12721 tttgtttatc cgtcattttt ctcacctact ttatcataca caatattagt tggataagct
    12781 atgaaaacaa aacaacagaa ggctgttctt tctaggcatc ttgctaaata tgatataatt
    12841 aagggacgta aaaacaagaa ggaggaaaat cctatgcaac agccattggc ttatcgcatg
    12901 agacctcgaa cgatcgatga agtcgttggt cagcagcatc tagtcgggga aggaaaaatc
    12961 atacgccgca tggtcgatgc tcgcatgctc tcttccatga ttctatatgg acctcctggt
    13021 actggaaaaa cgagtatcgc aagtgcgatc gccggctcta ctaattatgc atttcgcatg
    13081 ctaaacgccg ctacagattc aaaaaaggat ctacaagttg tagcggaaga agcaaaaatg
    13141 agtggtacag tgatcctctt attggatgaa gtacaccgat tagataaaac aaaacaggat
    13201 tttctgcttc ctcatttaga aagtggccga atcatcttga tcggagcgac tactgagaat
    13261 ccttatatta ccatcaatcc agcgatccgc agccgtacac agatctttga agtcaaacca
    13321 ttaacagaac aagacatcca gttggcagtc gaacatgctt taagggataa agaacgcggc
    13381 cttggacaac aagcaatcca gttagacgaa gatgctcttc ttcacctttc gcgtgcaacg
    13441 aacggcgatt tgcggagtgc attgaatggg ctggaactgg ctacactatc gactccttca
    13501 gataaagaag gaaggattca tcttacactg tcgatcattg aagaatgtgt ccaaagaaaa
    13561 gctcttaccc atgacaaaaa tggagatgct cattatgacg ttatttcggc ttttcaaaaa
    13621 tcgattcgtg gaagtgatgt ggatgctgca cttcattatc tcgcccgttt agttgaggca
    13681 ggtgatctgg caagtatttg ccggcgtctg atggttatcg gttatgaaga tataggttta
    13741 ggtaatcctg ctgctgcggc tcgtacagtg aacgctgtat tagctgctga aaaattggga
    13801 ttgcctgagg cacgtatccc actagcagac gtcgtcgtcg atctttgctt gtcgccaaaa
    13861 tccaattctg cctacatggc attagatgca gcacttgctg atattcgaga gggcaaagct
    13921 ggagatgttc ctgatcattt gagagatagc cattacaaag gtgcgaaatc actcaaccgt
    13981 ggcgtgggtt atcagtatcc tcatcatttt gatcaggctt gggtcaatca gcaatattta
    14041 ccagataaac tgaagaatgc tcagtattat cagccaaaag atacagggaa atatgaacag
    14101 gctctgggtc aacagtatta ccgtataaaa gagtggaaaa agcatcctcc aaaaaactag
    14161 acaagaaaac ggttctgttt ctgcgagctc tctcattgct ctttttaaat tcatatgcta
    14221 ctatagaggt gggaaatctg tgatgttcga attgtcctct ttgtgtgttg accgaacatt
    14281 tttattgata tcttgggacc cgtcttgtgt cactgctggt aacatgcctt atcatttggt
    14341 agttcgaagc tacgaccatg ggacccacct gcttgaattc gcgggatcaa tactacagga
    14401 caaacaacgg cattaacgga gattaccatc ttttaaagaa taaagcaatt tgctcttggc
    14461 aaattgcttt ttctatttgt tgcttatttt ggaggatttg ttcatgatta ggattttaat
    14521 gattatcgcc actttagtat tattattcgt tagctattat ttatttaata aacaggatat
    14581 cttttttgta ttgatcaaca aaaatgataa aaatcaagga ttcctacaat tttacggagc
    14641 tgcatatgct gttttaggcg taatgggtat tttaacagct tttttcaatc aacgctttat
    14701 tgccttggtc tttttactga tcgtaattat tgtttctgcc accttcagta tccgatttgc
    14761 aaaaaaaatt gctgaaccaa agcagtaaat aattctcttt ttccctaaag tgttctacaa
    14821 taaaagagta caatacgatt gacttgagga gtgaatgctt atgttacaac aatacaaaaa
    14881 aatcatggtt gccgtagatg gttctgatga agctgaatta gcttttaaaa aggcggtcaa
    14941 tgtagctatc cgaaataacg gagaacttct attagcacat gtcattgaca cacgttcttt
    15001 ccaaaccgtt tcatcctttg atggaatgct tgcagaacaa gctactgaaa tggcaaagca
    15061 aacattggca gattatgaaa gcaatgcgaa gaaagctggc ttgaataatg taacctctgt
    15121 cgtagaatat ggctcaccaa aacaaattat tgccagagaa atcccagaag ataatcaagt
    15181 agatttgatt atgttaggag ccacaggatt gaatgctgta gaacgtttat ttattggttc
    15241 tgtttctgaa tatgttatta gaaacgcagc ttgcgatgtg cttgttgtcc gaactgattt
    15301 agaaaaccaa ttgccagcta aagaccacac caaagcttaa cacgtagacg acctgccaac
    15361 tatcggcagg tttttttgca caaaaaaccg tgataaaaac aaaagacgcg gcaagcactc
    15421 atcggtcttt ataatgttta aaataaaggg aaaacagtca atgagttcaa gtaaaataaa
    15481 aaaaagtgaa caccagggcg attagtcgcc ctggtgtggt ttaaaacgac ctaagcatgc
    15541 caaagcttat tggttcgtca atgtggaagt ttccgatgaa tctgatacga aaaaatatga
    15601 agtatcgatg gcggataccg attttatcgc caaactaaaa ttttatttcg ggttcgggta
    15661 ccgcgagaag gcaagtgcta cattcggtaa atcattcaag aattgatgaa aggctaccgc
    15721 ttgccattgc aaccacaacg atactcctga acgcgatgtt ggtgagattt ccaaagagaa
    15781 gagctgttta atgcaacggt attaagcaaa tggcaaaagg aagtcatgta gacaaagttt
    15841 tttatcaaac accatatgat ttcgcctgaa cgctggttta gctcagaata caatgatgtg
    15901 gtccacgaga cattaccctc agtcagataa aaaaagctct ctgacagaat tacacatcga
    15961 taaatattaa gtaaatgtca gcgctagttg aactttctga cactttcagg tacactggaa
    16021 aagacaacta cacgaaggag ggaaaatact atgatgcgca tgactaaaat aacaagtaat
    16081 gaaaattacc aacgatacca tatcgcattt agccatgcaa ttggttttac agttgctgtt
    16141 gacgggacaa gtttgtccaa aacagttgaa ttcacgtaaa atcaatggcc atctcaaggt
    16201 atttttctca tgataatctg agtttaaata acacaggcca tggataacca tggccttttt
    16261 gagtggtctc aaggagagag atcatgatca tacaagcaat ttctattaaa aaaacaatcg
    16321 gcgggaatcc attatttgag gatttatcct gtcaaattga agctggtgaa aaaattgcct
    16381 tagtcggtat caacggaact ggcaaatcaa cctttttaca aatcttgacc ggcagagaag
    16441 gtgtcgacag cggtgttatc agtcgcaaaa aaggcttaaa gatcgggacg gtcgaacaag
    16501 aacttacagt ttccgaagcg accgtcaacc actacttgct ccattctgcc gctgagatac
    16561 aggacctcaa acagcaaatg agtcgctacg aagcattgat gacagaacca gatacaaact
    16621 tagagaagtg tctggcccgt tacggtgagc tgcagcatcg ctttgaagaa ctaggcggtt
    16681 acgtattaga agaccggatt gcagcgatct tgcaaggctt agggattcca caccgtcagg
    16741 aagcacggtt aacagaactt agcggaggcg aacgagttaa agtcgcttta gccaaaattt
    16801 tagcagccga tgccgattta cttttactcg atgaaccgac caatcattta gatcttaaca
    16861 gtattcgctg gttggaaaac tatttacaga atacaaaaaa atcggttctg gtcgtttctc
    16921 atgatcggca atttctcgat caagtcacca caaaaacatg ggaactggaa gaaggtgcat
    16981 tgattgagta tcccggaaac tacagccgtt ttcgagtctt gaaagaagct cgcttggctg
    17041 agctgacaaa aaattacgaa ctgcagcaaa aagaagtcca gcggttgaaa gtcatgattc
    17101 gtcgttttcg tcagtgggca catgaaggag acaatgagtc cttcttcaaa aaagccaaag
    17161 aactggaacg ccgcctggcg aaactgacac tcgtcaaacc gccacctccc ccgaaaaatc
    17221 ggctgcaaag cttgtctaat ggtggaaaat ctggcaaaga agttttcatc attcaaaact
    17281 tgcaccagca gtacgctgat caagtactat tcaaagacag cagttttgca gtctatcgtg
    17341 gagatcattt agccatcatc ggcgacaatg gtgcaggaaa atcaacctta ctcaaattat
    17401 tactcggtga ggaaaagcct tacggtggca ccatcaaatt aggtagtagc ttgcaaatcg
    17461 gttatttgcc gcagcagctg cagtttgccg atcccgatag ccgtttgctg gcttacgcca
    17521 tcacattgac tggaaacgaa gaagtcgctc gacggcaatt agccaaaagt ggcttttatc
    17581 aaagcgatgt gggtaaacgg atcaaagatt tatccggtgg tgaaaaaatc cgtttggcgt
    17641 tgctgaaact ctttttagaa aaaatcaacg tcctgatttt ggatgaacca accaatcacc
    17701 tcgacagcta tgctcgagaa gaaattgaag aaatgctgca agactatcaa ggaacgctcc
    17761 tagcagtcag ccatgaccgc tactttttgc agcaacactt ccaagaagca ctcgttattg
    17821 atcaggagca gatcagccgc cagccgctcg gccaactgat tgactaggac gttttttcgc
    17881 aacaaaaaac ttgattttca tacctgtgct ttcacgattc aggcaaagta caggtggaaa
    17941 atcaagtttt tattattcga tcaatgaatg tttcggatcg gttttctagc taaacataaa
    18001 gcgaagaata aagtcgacag cccacgaggc tgatctaaaa atgttttttc tttttacata
    18061 tgtaatgtcc cttataaaga tgaatgaaaa taaaatattg ggattttcct gaaaatcaat
    18121 aaataaaatg ccagtctttt cccttttgac cacttttcca agcaaaattc cctaagaaga
    18181 tcctgtaaaa aagaagggaa attgaagtta aattaccatt ttgcggtctt ctccgcaaaa
    18241 aaaacacatg caagaattcc ctcttgcatg cgtttcatct tttatcctaa cgaatgcttt
    18301 ggcttagccg cgcagacgct ccacatcacg ggcaatcatt agttcctcat ctgttgggat
    18361 cactaatact cttacttttg attcttttgt agagatatct acttcttcac cacgcaattc
    18421 attcaacgct ggatcgattt cacaaccaaa ccaagacata cctttgatga tttcactacg
    18481 tacatgtccg tcattctcgc cgataccagc agtaaagaca attgcatcaa caccattcat
    18541 cgttgtcaca taaccgccga tgtattttcg gatgcggtcc acgaaaatgt cataagctaa
    18601 acgaatatgt tctttttcat agttcttttc aagatcacgc atatcactag acaatcctgt
    18661 taggcctagc agaccagatt tcttgttcaa aacattgatc atttcatcaa tatcaatctc
    18721 taatttttcc attaaatatg ggagaacagc tggatcgata tcacctgaac gtgtacccat
    18781 tgtcacccca gctaatggtg tgaatcccat agaagtatcc atggatttac ctccatcgac
    18841 tgctgtgatc gatacaccat ttcctaagtg gcaagtgatg atttttagtt cttcgatcgg
    18901 tttgttcaat aattttgccg cacgttccga cacataacga tgacttgtgc catggaagcc
    18961 atattttctt actttgaaat cttcataata agacaccggt aagctatata aataattttg
    19021 ctttggcatt gttgagtgga aagatgtatc gaatacagcc acactcaaaa tgtcaggtaa
    19081 aatcttttta aacgctttga tccccattaa gtttgcagga ttatgcaaag gagcaaattc
    19141 tgctaattca gcaatgcggt ccattacttt ctcatcgatc actactgaat ccccataagt
    19201 ttctcctccg tgaacaacac ggtgccctac tccggtgatt tcatcataag agcttaaaat
    19261 ttgcagttca atcaactgat ctaacagcat ttttactgct acttcgtggt tttcgatatc
    19321 catgatttgt tcgaattttt gattatcgcc gtacttgatg gtaaaaatag aatcttgtaa
    19381 accgatacgt tcgacgatcc ctttcgcaac tacggtttct tctggcattt gatataattg
    19441 ccatttcaaa cttgaactcc cagcattgat tgcaattgtt ttagacatat gactctcctt
    19501 ctcgttgaat gattacagag cagatgattt ccagttttta aactcgttga aaaattccgt
    19561 caccttctct ggttctttta atgaagcaag tttcacaagc aagacttctt ttacttgttg
    19621 tgcttgtggt ccttttttct gtaagatcaa aatacttttt tgcgattgtt tattacggaa
    19681 caattcatct ggcaattgaa tcattccttg aagataacct tcttctttaa accatttttt
    19741 gatttcttca ctttgatccg tttctaaaaa tccactcggc atcaaaaaca aaccaaaacc
    19801 atccggcttc acatatttca ttgattgctc tagcaataaa tgatgtgcat aactatggcc
    19861 ttcttcggta cttgttaaaa actcttttgc tttttcgtca ttcggataat aaccgattgg
    19921 caaatcgctg attgcaaaat cgacaggatc tattaataat tcctgcaacc cgtcttgatg
    19981 aaagtattga acatttgctt gtgtcaaatc acttgtagaa gctgcgacag ccaacaatgt
    20041 atcatcaata tccacaccaa ttccttctgt ttggtacccc gcattcgata ggtttaaaag
    20101 aactgttaat aacaagttgc ccattcctgc cgcaatatcc agaatcttaa ccggtgcttt
    20161 tttatttgtg tatagttgtt caataagaaa aacaaataaa aaaccgatac tatcaggagt
    20221 gagttgatgg ttcgcctgca aatgttctgt ctggctgccc ttcaataaaa gtaattgaga
    20281 cagtctgcgc cattcttctg gctcgaaaga aagtttcttc aactcttcat aaagagctgt
    20341 gatccgttgc gttgtctctt ttgtcggcac tccatccacc acacgtactt gataatcatc
    20401 tattaaattt tctgcatttt cgacataggc ttctaaaaag gaagttccca atgcattctg
    20461 taaaagctga atagcttcca ggttttgatt aaacccttgt tccatttttt ctggtaacat
    20521 ggtgcacctc atttctagat atattataac aattttttca cctgtttaat tctatcgaac
    20581 aattgacaaa atttcaatcg ttttcactgt tttttatttg tgaaacggca aacatttttt
    20641 tattaaaaac agtgttataa cagaaaagag ggcgttttca cgaacattcc atcattctgt
    20701 actagtatct gtatcatttt ttacttcttc tgtgaaccga aagataaacg gtgagacttc
    20761 cacctctaag aatagggact gttgtaccca ctgatatgtt acagatccag ttgtgtagta
    20821 aaaaactcct tccgaacttt cggcaagcgt agactgttcc gataaagcca tctgcttcat
    20881 tatttttgct atataataat ttttgattcg tatattaaga tccattgaca aacggtagct
    20941 ttctaatgct gccatcaata aaaacgtaca taaaagaaat aaaagcaaga cagaaaataa
    21001 aatagagccc ttataacatt ttctactgat cgttttttgt ccatatggca tatccatctt
    21061 ctccattttc aaagtgtaca taaaaagaaa ttgtgtgttc tttttccaat aattgaagct
    21121 cagtgatact tgttaacata ggttgatgtc cgccggaagt tcttctttta cgtatcattg
    21181 cctgatatct ttcgatgata tactgtttat cctcgatcgt gtagtgcagt tgctgatttt
    21241 tcacagagac attgtgactg ctcttgagtt cttcttctaa ttgtgctaaa aatattagcc
    21301 actctttttg atctttacgc tgcaaaaatt cacttacttg gatggtttgc tgaatcaaaa
    21361 gattgaatgt gagcaataga ctgctcaata tcatcagtcc aattagacat tcaatcagcg
    21421 taaatgctgg aattacttgt tttttcaata ctgatcgctt ctttctccat attgacaaac
    21481 actttttcga ttactccatt attatcgaca atttggatat cgtatgggat catttcttcc
    21541 cttcgctctg gtcttttgtg atacatccgc tgcgtcttga tatcttcata aatcattcga
    21601 ataaatagca gttcctcccc acttttcttt tctgcctgaa gcaaaaacag ctggccaaag
    21661 ctcactaagt agacacagac agaagcgatc ataagcgctg taagggactc caacaaaaca
    21721 aatcctgttt tattcaattc tttttgtata gcgaccactc cctatctgga attgatacgt
    21781 aatcgtccgc tctttttctt cccaataaaa ttgataagct tttagtgaac tttcattccc
    21841 ggttctagca gcaaaagtaa tagaagcatg gcgtttcaag gtaatctcat caggaatatc
    21901 taatacttcc caatttattt tatctggatt cggaacatga aaaatgattt gattctcttc
    21961 actattccaa taaaagcctg tttggatctg attcacaata gcgatttttt gcgtagaata
    22021 gattcgtttt tcaaattggc cgaaaaattg atagactgcc aattcattct tccatgcaga
    22081 aaatgcgaga actggaaaag aaaggatcgt tgtaagtaat aaaatcagta agagtgattc
    22141 aaacaacgta tatcctgatg ctaaaacctt accttcaagg ccgctgatag atttctgcat
    22201 gttctcgttt gatatagcct tcattcaaca attcctccac agtaggaact cttccattat
    22261 tttgaagcgc atagagctct ttttgtgtgt ctaccaactg gatgatcgct tcacgacttt
    22321 cttggtctac atgattccta taacgcgaca aattagggac gaacaacaac actaatacac
    22381 tgatgatcag taagacgatt agcatttcga tcaatgtaaa accgctataa taagatattt
    22441 ttctcttttt catggcaaaa cctcctctat gcttccgtaa acaggtaata aaacagcggc
    22501 ataaactaat aaaatcaaag ccgctacgcc caaaaaaatg attggctgaa taaaatgcat
    22561 ccagtgatga agacgtttga aaagctgttt tcttgtccat tcgctgtaaa aaatcagttc
    22621 tttacctaaa cttccctttg cctctccctg taaaacaatt ttactgaatt cttctgatag
    22681 aaaacgataa ttctggaact gctcagccaa tgacatacct gcctccaatc cttgaatcaa
    22741 cttagaggca agatgctgaa tcagactttc ttgtttcgtt tcctttaagc agactatgat
    22801 ttgccttaat tcgaaacctt catgaaaaag ctttccaatc tctagtgaca aataagccga
    22861 ttgatacaag gaatatatcg gtccaatcaa taattgtctg cttatccatt cactctgatc
    22921 aacaggagac aacttcatca gacgatacct tactagtaaa gccaatagag taaccgctag
    22981 gatgaatagt agaacatacc aatgaaagac ttgtatcagc tgaatacccc aatgctcttg
    23041 catgaccatt cctgaaccaa taagctgagg caaaacgaac aaccgcatac taaacaagat
    23101 acctagcaaa aaggtgagca atagcatcgg atatgcaagc agctttttca aatcattgcg
    23161 aaatccttca acaagccgaa actgtttggc aatttccaac aaagttgtcg gcagattccc
    23221 atgacgttca gctaactcga tctgtaccag ttgctctgct ccatatccta tttgcgaaag
    23281 tacctcacta aacgcttgcc cttcctttag ctgatgttgg atatttgcca aaataacagg
    23341 tggaaacgaa cggatcgtga gtaataaata aagggattct tgcagactaa aaccagataa
    23401 aagcagttct cccatggttt gtgcaaactg gttttgctgg cttcttgtta atcctttaga
    23461 atagttctga ctgccaaata ttttcctgag cttcttcata actttcctcc catgattttt
    23521 ttactgattc ttcctccata aattgataac tccataaaac agaaggatgg tgattggcat
    23581 cttccaaaag ttcttgataa atgacaccag ctaaacattc agatagctct tcttttttct
    23641 ttgtcagttc atggattctg accaatgtac cttcaagatt gcgtgcatgt acggttgcaa
    23701 aaacccggtg ccctgttagt gctgctctta ccgcagcatg tgcagttgat tcatctctta
    23761 tctcgccaat gattagcaag tctggacgat gccgtaatgc gagtctcaaa agtgtatcat
    23821 aagtctgtgc tattttcgga ttgatctgta gctgcagaaa aaacggttct tctatctcta
    23881 caggatcttc gatagtaatc acttgcaatt tttcttccaa agccaaccga tacattagag
    23941 aagtcttgcc tgatccgact ggtccgctga aaaggtataa tccacgtctg acggttttct
    24001 gtaaaatagt tgcaaaatct cgagaactaa agcaacgatg gggtgtttca aaagtaccgt
    24061 ataatagccg aatcactaaa ctctctcgtt gaaggtaatc tcctacagaa gacaaccgca
    24121 gacgaacttc ctgtttttct aaagaataag taaatgcacc taactgagct tttctttttt
    24181 ctccgatatc catctgtccc aaatacttaa atcttgagat cagttgctgt ccatctgccg
    24241 aagagacttt cttccagaat gttcgttttt ttccatagcg aaaatagatg taaaatactt
    24301 ctgaagctgg caatatatat acgtcttggg catgtttggc aaaactttca cttatcattt
    24361 gatccgataa tcgttttatt tccattattc tcccctccac aaaaaacata cgtctttttg
    24421 atctttttgt ttcaaataaa atgaatttac aaaaaaatat tggcaaaaac cgaatttatg
    24481 ttatacttat aaaaatttat cttgggaggc actccattgg aaacaacatt aaggaaatat
    24541 atcgaagtag caagtaaaaa aaagcctgct gacctagtga tcaagcatgc taagatcgtc
    24601 aacgtcttta cgaaagaaat catggaaaaa gatgtagcaa tctgcgaagg aatgatcgta
    24661 ggaatcggtg aatacgaagg aaaaagcgta cataatgcta atgggcaata tctagtgcca
    24721 ggattcatcg atggccatgt gcatatcgaa agctcacttt tatcaccaag cgaattttca
    24781 aaagtatcct tgcttcatgg agttactact gtagtgacag atccccatga aataggaaac
    24841 attgctggga gtacaggtct tgagtttatg atagaagatg caagacaaac actcatgaat
    24901 atctttttta tgctaccttc ttgtgtacct gtaacccctt ttgagaccaa tggtgcaaat
    24961 ttaaatgcaa aaaaattagc ccctttttta caaaaacccg aagttttagg cttagctgaa
    25021 gtcatgaact atcaagcagt agcaaataat gaaacagaca tcctagaaaa gatccaattg
    25081 atgcaccagc ataagaaaaa aattgacggg catgcagctg gtatcggcat ggaagagtta
    25141 aatgtttatc ctgcagcagg tattcggacg gatcatgaag ctacaactgc aaaagaagct
    25201 aaagaacgtt tggatcttgg tatgtattta atggttcgag aagggaccgt tgcaaaagat
    25261 ctggcatcgc tactaccggc gatcacacct gaaaattcaa gaagatgctt ctttgtcacc
    25321 gatgataaac tgatcaatga tttagtaaaa gaaggatcaa tcgatcatat cgtccgtcaa
    25381 gctattgcac ttggaatcga tccattacaa gccattcaga tggcaacatt gaatgcagca
    25441 gaatgttttg atttgaaaac actaggtgcg attgcccctg ggtatcaagc tgatttcttc
    25501 ttaacagacg atcttaccaa gctgccaatt catgaagttt tctataaggg aacattagtt
    25561 gttcaatctg ggcaactcca agaccattta tttgctgatc attcaaattc atatacgtgt
    25621 tgtctgccaa aactaaacgc tcaaccatta aatccacatt cgttagagct gcttctttct
    25681 tcttcacaag cccatgtgat cgaaattatc ccgaatagtc tagtgactaa agatttggta
    25741 gaaaaagtgg accgacaagg cgaacgattc attccttcaa tcgaaaaaga ccaattaaag
    25801 attgcggtga tcgagcgtca ccatcaaacg ggaaacattg gattaggcat agtgaaagga
    25861 tttcagctga aacaaggagc gatagcgaca acgattgccc atgactcgca taatctagtt
    25921 gtcgttggga caaacgacga ggatatgctg tatgctgcta atcttttgat tcaaaaaggt
    25981 ggcgggatga ccgttgtcaa caaacagcag gaaatagctt gtgtttctct tcctataggc
    26041 ggaatcatga cacaggaacc tttttcgatc gtctatgaac agttgaatga attaataact
    26101 aaagcacatc aactaggagc agctaaaacc tttgatcctt ttttgacact ttctttctta
    26161 acattatctg tcatacccga attgaagatc accgacaaag gattattttc attttctcgc
    26221 ttccaattga ttccaccttg tggaaaaacc gatgaaataa atacatttta aatcaaataa
    26281 atcccagaaa atacattaca tctgtaatgt tcattttctg ggatttattt atggatatta
    26341 atgtttttat gaaagcagaa agttagtttc acagactttt ctttttggtc ttatcttgaa
    26401 attgagcttc tactcgataa atacgttgac cttttgaaga aaatttttct tcatactccg
    26461 tcataatatt gccttcaaat tgactttgat gtaaatctaa ccatacctgt ttgaggatca
    26521 tgccatattg agaaaaactt gccagagaat actcaaacaa tccttgatta tctgttttga
    26581 aatggatttc tccatttgga cgaagaatct gttcatctac agcaagaaaa gtcttatatg
    26641 tcaatcgccg tttttcatga cgtttttttg gccatggatc ggaaaaattc agatagattt
    26701 gatctacttc gctgtcagcg aaatactctg tcaacgctga tccatccaca tgtagaagtt
    26761 tcaggttagg aagcggttgt tcgatcaact tgtctagtgc aatcgaaaca acactgacct
    26821 gcatttctac tccaatataa ttgatttcag gatgggcttt tgccattcca gtgatgaact
    26881 ggcctttccc cataccaatc tcaatgtgga ttggatgatc gttttcaaac aattcattcc
    26941 attttccctt ccacaatgtt ggatcactaa ttacaaaatt cgggtgagcc gctaacattt
    27001 ccgctgctcc cggacgattt cggacacgca ttatttatct ccattccttt cctctaatta
    27061 aaaaggtctg gccaaatctc cgcagagttt gggccaaacc tcagccactg aaaattcgtt
    27121 ttaattgtaa aatttcttcg ttcatccgtc gatgatcttc acgtttgcaa tatctggtga
    27181 tttcttggag caaattcatg gccgcatacc aatgaaattt ttcaagcatt tcgtcagtcg
    27241 ggcgtatgcc ataactgatc agccactgat tccagctaga cagtggcaca taatttccta
    27301 aaatcgttgc aatatccacc gcagggtctg caaacatgat tgaatcccaa tcgactagat
    27361 ataaataatt tttacatact aaccaattac gatggtttac atctccgtgt actactgata
    27421 agttttcctt atctattgac gggatctgct cacgaagaaa acgatggaca atttgcagaa
    27481 attgattttt ctggagttct tccggtaatt ctttttcata tttttgcagc atcttctccg
    27541 gcgtcatgac acgaccaccg atacgggtca gcatggtttt aagagaattt gaatgatgta
    27601 aatggtacaa aacatcaatc acgtcgtttc tttgaccaat ctcattagga gtcagcaagc
    27661 gaccttccag ccactcttgt gctgtcaaag tatcgccatt ccccgttcgt tttgtccaaa
    27721 caagttttgg tgtaattcct tcccttgaaa gagcagccaa catcggagtc gtgtttcgtt
    27781 taataaagac tttgtcctca tcacgcatgc ctatgtatgt ttttcctgtg tcgcctttga
    27841 tgggacgtaa ccgccactct tggtctaact gaaactccat catgcattct cccgtcctct
    27901 tactctttgt tttgcagtac ttattattct agtcgcctga acactacacg tcaagcacat
    27961 atacgttctt tttcatttaa ttgccattca aaaaaccagc ttttctcaaa aaaaatagaa
    28021 cgaacagcat tcttactgtc aacgctctat ttttcattct catttttacc ctacacagct
    28081 ttgttttcag tctgacatct tcttcaaacg agttggtaca tatagtttaa caaagagcat
    28141 agtaacaatc aaataagccg ctagcggaac ccatcgctgc tgcccatcta atacgactgc
    28201 agcaatcaaa ccaaaaataa ccgacgcgat aattaatacc catgtgagta gatactgaat
    28261 cgcctgcttc ttctgattct tatggattgg ataaagctga acgagaacca tatagcggaa
    28321 ctggttcgct aaaggtaaca gttggaaacc gatcaaatat aaaaataaga aaccgataat
    28381 cagactaaaa taaaagtcga ctactccggc aatcaataac ccacctataa ttactaatcg
    28441 gaaataaaga ccactaaaat cagaacctct tgccaatctt cttgcaaaca agtaaagata
    28501 agtgtttttt gattctcttt taatatgagt caatatagga tccaaatagc gtcttctttt
    28561 gatctttgcc gtaatttcag gaacatctgt gaataaattg ataaattgat agatctgatg
    28621 caacctatgt tcttctacat gcaccatttt ctcccaatcc aatccttttt cttgttgctt
    28681 ccacaaaaac cagtaatata gacttgcctg tactaatgca cctgctattc ctgcccatat
    28741 tagccaataa aatgatagaa acaaaaagat cagagaagaa aaaaaccagc ctaatttcca
    28801 ataacgaata acttgttgat ctagtcgaaa caattctgct ctcttgattt gcagatgact
    28861 gaatttcaaa ctccataata tgatcagata gacgaaaaac atcgaaaagg attgctccgt
    28921 actaaccacc actaatggca tggccaatcc gcatgccaat atcaaaaaga tcaacggaaa
    28981 aatgcaagaa tagcgaaaag ctcgttccaa ataggtttgc atttcttttt ctttaggaag
    29041 taaaaaaaca atatctgctg cctctgcaaa agtagccatc ttacccacga ataaacagag
    29101 catccaaaat acagagatta tgagccctcc aattgcaaac ggtggctgca gggttttcaa
    29161 ccaattagaa taatataatc ccacaccgcc aatcaagaaa agacaaacta aaacaaaatg
    29221 atcgttcaat acataacgca tatatttcat cattttcttt tgatgtcttg ctaaacgttt
    29281 ttgaaaaaaa ctactcatgt gcctgctcct ttgttaaagc aatatagatt tcgtccaaag
    29341 aagaatccgg cagctgaaat tcttttctca acatctcaag agaaccgtct gctcgtatct
    29401 ctccgttatg taagaccaca aaacgatcac aatatttttc tgcagtagct aaaatatgcg
    29461 tagacatcaa aataccggct ccttcttttt tcatttctac catcaattca agcaatgcat
    29521 tgatagctag tggatccaag cctaaaaatg gttcgtcgat gatatataaa gaaggctcga
    29581 tcaaaaaggc gcataagatc atcactttct gtttcattcc ctttgaaaaa ttagctggga
    29641 accagtcaag acgattagat aaccgaaaag tctccaacaa cttttctgct cttttcattg
    29701 ctatatccat aggtatatcg taggccatcg cagtgacttc gatatgttct ctcaatgtca
    29761 attcttcata taaagaagga gtctctggaa tatacccaat ttgtttgcga tattcttccg
    29821 tatttttctt taaggataga tgattgattt ctatcgttcc ttttgaagct tccagcagac
    29881 cgataatgtg cttgatggtc gtacttttcc ctgctccgtt cagtccaatc aaccctacta
    29941 tctctccagg ctgtacatcg aaactgatat cttttaacac agggagatgt ccgtaccccc
    30001 ctgtgacatg atcgataatt aaactcattc tttttcctcc gccatttatt tttatttatt
    30061 ataccacgaa gaaaatgacg gtattttgtt tttttcctta caatagcttt aacttatggt
    30121 aaagttaagc tgaagtattt attctttata gaaaggatgt tttatatgga aaactgtatt
    30181 ttttgcaaaa tcatcaatca agaaatccca agctataaaa tatatgaaga cgacaaggtc
    30241 tatgcttttt tagacatctc tcaagcaacg aaaggacaca cattagttgt gcctaaacgt
    30301 catgtagcag atatttttga atatgatccc gaacttgcag gagaggtttt ttcgcgtgtg
    30361 cctaagattg ctcaagcatt ggaaaaagct tttccagaaa tgaaaggtct taatatcttg
    30421 aataacaacc gtgaactagc ctatcaatct gtttttcatt cacatatcca tttagtccct
    30481 cgctacagca aagaggatga tttttctatt cactttgtca atcatcaaga tagttatgga
    30541 tcggaagaac taaaagctat tcaagaaaca atcgtaaagc aggtgtcctg cgatgattaa
    30601 aaactttttc aaaggactgt tatttggagc tgctgctggc actgtcggag gtctgttatt
    30661 ggcgcccagg agcgggaatg aaacaagaaa gaaactgatc gaagaattgg aagaagcaac
    30721 aactctgaca aatgatttaa ataacagcct gaatcatttt aaaagcgcat tgatcgaaac
    30781 aaaagaaaca gctgaatctg tgattcctgc tttccaagaa gcaatcaaaa aagatatcga
    30841 agattttaaa ttccaagccg aaccacgtat cacacaaatc aatgaacaag tagaaaaact
    30901 aaaagctgat ctgccagagt atcctgaaca aatataataa tgattatttt ctcaacagat
    30961 ttaactatat aagtgagaaa agtttgcgta tttcctagga atgtgatgaa attttgaatt
    31021 ttgtttgaaa ctaccacgac aacgttttta aaggaaatgg gatatggtat agttaatata
    31081 taaaaataat aactaattac aggagtgcaa attttaatga aaaaaaaatc aattattttg
    31141 gcagctacta gtgcgttggc tgttttaact ctagcagctt gttcaggcga tactaataaa
    31201 gatatcgcaa cgatgaaagg cggaacgatc actgtatctg atttctatga tgaagcaaaa
    31261 cttgaatcat cgaaccaatc cttagtacaa cggatgatca tttataaagt attcaataac
    31321 aaatatggcg ataaagtaac cgataagcaa gtggatgcag aatacgacaa acaagcgaaa
    31381 tcattaggtg atacttttga atcacaatta gaagctgctg gatatacgaa agatacttat
    31441 aaagaatata ttcgtaacaa tttagctttt gaagctggct tgaaagctca cgtagatatt
    31501 acagatgacg atttgaaaac tgcttggaaa tctttccatc cagaagtaga agcacaaatc
    31561 atcaaattat caagtgaaga tgaagcaaaa gatgtgaaaa aatcagcgga tgatggagat
    31621 gatttctcta aattagccaa agacaaatca actgatacag agacaaaaga agatggcggt
    31681 aaagttaaat tcgactcaac gacaacaact attcctgcag aagtaaaaga agcagctttc
    31741 aaattgaaag acggtgagat ctccgatgtt attactacaa caaatcctac ttcttatgcg
    31801 acagaatact acgttgtcaa aatggtgaaa aaccaaaata aaggcaatga catggataaa
    31861 tacaaagatc aactaaaaga tatcgctact gaaactaaac taagtgacaa tgcattcaca
    31921 actaaagtaa tcggtgaaga attaaaagat gcaaatgtaa aaatcaaaga tgatgccttt
    31981 gaaaatgtgt tgagtgcatt cacaacaact tcatcaagta cgaaagactc aagtgaaact
    32041 acagcttcaa caaagagttc tgacacaaaa tcaactgatt caacaaaaga atcttcaact
    32101 gaagaaacaa ctgattcatc aaaataaaag aaaacaaaaa aagcgaagac ttatgtctcg
    32161 ctttttttgt tttgaatgaa aaaaataaat ttttgctata aatcttttgg tacatagaaa
    32221 cttcggttgt ccataccgaa aatccgatcg gtgtattttc ctggttctgt ttgacgaatc
    32281 gaagtcaaca tcatctgcat cgaagcatcg atattgtcga tttgatgtaa tatttctgct
    32341 tccattattc ttggacgtac aggtgagcca tattctaata agccatggtg tgctaaaacc
    32401 atatgacgta atacgagaac atcttcgtca gcttcgctga ttttcaacgc aagacaggct
    32461 ttagttattt cttcgtcaat caataccaga tgaccgatca aatttcctgc aagcgtatat
    32521 tctgtcgaca taggaccgga aagttcaatc acctttccta aatcatgcaa aatgatacca
    32581 gcatacaaca atgctcgatt cagctcagga tattcatcgg cgattgattt ccctaagtga
    32641 agcattgtca aagtatgata agctaatcca ctggcaaatg catgatggtt tctttttgct
    32701 gccggaaact caaaaaattg tttttgatat ttattaagta gatagcgaac aatacgattc
    32761 caatgcgcat tggtgatctc aaacaacaat tgattgattt cttcttccat ttcttctttc
    32821 ttcaaagggg ctctttccat gtataaacta ggctctcctg gctcatctac ccttgttaag
    32881 cgcatatgaa gaatctttac ttgagggttt ccttgataaa cctctctttt accgtttaga
    32941 aaaacgactg tgccggctgt aaatttattg atttcctcct ccgaagcatc ccagtattta
    33001 ccatcgatag tacctgaagt atcttggaaa gtaaaagcaa taaatttttt tccgttttta
    33061 gcgattctta catctgcact tttgatcagg acaaattgtt caaactgctc atcgattact
    33121 aaatcacgaa tttttttcat tatttgctcc ttctaattgt tttacgattt ggttcatctc
    33181 atggtaatag cttaccattt ctttatcaga agaaaaacaa attatttgat acttttctcc
    33241 aaatttagct aaaagttcag ctaattgata ctttcgctga tgatcataat gaagccagcc
    33301 atcatcaatg atgatcgggc atatcgaacg gttagcttct aatgagaggt aagcaaatct
    33361 catcgccata atcagctgat ctttcgtacc tgtagataac tcatagatgg gaaattctgt
    33421 atatccgtct gttaaagtca gtgtatcatt tttcagtaat atcttctgat aatttccttt
    33481 tgttaaaata cctaaataat cacctgctgc tcttaacaac tggggtaatt gtctttcact
    33541 catctctgtt gaaagatcac ctaagaatgt cccggcgatt tcaagtgctc cccattcctc
    33601 cagtaattcc tgtaattttg ctttttgccg gctagcttgt tgataaagtt cgtccaatgt
    33661 gccgtctctt tgcaactctt ctacttgaag ttggagtcgt tgccgatttt cacttgcttc
    33721 tttttcttct tgctggagtt gggattgctt ttctttcagt tggcgcagtc ttacagataa
    33781 tttctctttt gtcagctctt caggaaatag tggagtaagc atttccgata attcattgta
    33841 tcttttcatc tgctgttcgg cttgtttggc tttttgtaaa aagacaggta cttccgaagg
    33901 ataagataag cctgcttgag tcaattgttc gctgtattct tgtatccata ctttttgttt
    33961 ctttttcaat tgattgattt cttgcttcaa taaagtattt tgctgatagc ttctggcaaa
    34021 tttcatctgt tccatctctt gtataaaacg ggaaagcaat accatttttt ctgaaattgt
    34081 cttatcttgt atcggtaacc attctttaac aaactcaaat ttttggtcga tttgtcgtag
    34141 tttttcttgt atttcctgtt ctcttttatg tttccgctca aatacgccat ataatttctg
    34201 ataatctatg acttcctgtc catattcttt catgagtttg agcgtatcca tctttcctaa
    34261 gtgccgatga tccgcaaaac gatatacttc ttgctgaaga atctgttttt cttctccagt
    34321 tctcttttgt ttttcttctg cttcagctaa ttgagctaag gctagatcaa gttgtcctag
    34381 tttttcttgc cacattcctt ttatctcaga gaaatcattt ttagttttaa tttttggttg
    34441 ccttttgtag aagaaatagc caatcatgcc tgcaccaatt agtaaaagga tcactttcaa
    34501 gggcattggt acaaatatac ctgcaatgat tattgctccg ccagccatca gcatatacgg
    34561 tttagatgaa gagactggtg ctatctctga ctgattttgt aataatgctg gataggtctc
    34621 ttccaagtca ttcacttctg cttcaatcat ctcttgctgt tctttagcta tttggagacg
    34681 cacagaaatc tggctttcta gatcagcaat ccttctttct tcagccagaa gatgcttcca
    34741 ttcttcttca tataaaaatt cttccggtaa cggatttttc tgccaatgcc acctttgttc
    34801 caaaatcttc aattctgaat ttgtttctct agtctcaatt tgtaatcttt gccattcttc
    34861 gtttaattgt tccgctgtaa attgaagttg ctggagatct tttagttcct gctcctgctc
    34921 taaataaaaa taatatctgg ctgactgctg atccatcccg ctatgctttt ccagtgttgc
    34981 ttctaattct tccaaacgct catttgtttg ctgataccgt ttggagaaag actccagatc
    35041 taaaagaagt tgctcatcag gaagacctgt agtgctgttt tttttgagag attgccattc
    35101 ttcatataaa gaaaaattaa gttcttgctg tctggctaat gaaaattgtt tgttcgcctc
    35161 ttttaattgt gccaataact gctgctgttg cgactcgtat tcctgttctt gttggatcaa
    35221 cttgccaaaa taagcttcct gttcttgttt ttgatgaatt ttttcttgca gctctttcca
    35281 ttcatttaat ttttgattga ttagtaattt ttggcctttt ttcttgaata attgttgtgc
    35341 tttctgataa tattcttgcc gcttttggaa tacttgagaa ctgcccgtga tgcctaaaga
    35401 aatcagcgca tcatgtagct ctttttctct caaatgatct agttccatca actgctcttg
    35461 ctggaaagta aatacctgct ggaataactc tttcgtcaaa ggtgcgatca gtttttctag
    35521 taactcatca cttcccactt gatcccccag ccacaccttc gattttccgc gattttgctg
    35581 cttgtagcgc tcgacagcaa attcaccata tacaggatgt ttaagccata ttttgccgcc
    35641 ataggctgag ccgtcttttg gtgtataatc ctttttttta cggccttttg aagggaaacc
    35701 aaataatatt gcttgaataa attgataaat cgttgatttt cctgcttcgt ttcccccata
    35761 gatcagctga ttgcctgaat aaaagtcaaa acttttttga cgccattttc caaaagcagc
    35821 aatctcgatc cttaaaatct tcattcctga tcctcctcaa ataaaaaatc ttgattaagc
    35881 agctgcttgg ctttttcaag tgtttcttct tgatattccg gcagttcgct caatattcta
    35941 gccgcttctt gatgactata tacttcattt aaaatttgtt ggaaatcttg aggtgtctga
    36001 tagtattgaa ataactgttc catcaattta gcagaagcag caagtggggt cttcgtataa
    36061 aaagtattct ctatgagaga aatacgccaa ataaagaaat catttatcct atctgagaat
    36121 tcctctaaca aatagtctaa tagctctcct gattgtatcc gttccaaaac atctgtattg
    36181 agatggtcat aatcttttaa ttttatctca attaaactaa atccatcctc tacttttgaa
    36241 agctgctgac gaatgtgaac aatgacctct tttgtcgttc ttgctttcct caatgatatt
    36301 tcctttgtct tccaatagac ctctgctact ttaatcggat gtatttggca acttccttta
    36361 cttaattcaa ctagtagaat agatgtagaa gcttgttcct ttttagtatg accttgaggc
    36421 gcacctggat aaacaattgt ttgcttttca tttaagaccg ttggcacatg gatatgccct
    36481 aaagcccaat aatcgtatcc cttttcttgc atctttgagg gttggaaagg agcatagttt
    36541 cctttttgag agacgccagg ttccccgtgg tacaacccaa tatggtaatc tgtgagttct
    36601 ctagaaggaa attccatcac tttgtctttt tgaatccacg gttgacgata actaaatcca
    36661 ctgagagaga cagtttctcc actttttatc gttatttttt ttgtctctac cgtttcggat
    36721 gtaaataaat ggacattttt aggaaaggag aaccaatacc gctctttttg atagaaatca
    36781 tgatttccga atattataaa tacaggtata tggttctttt ctaaccgttt catctgttct
    36841 gaaaaatgtt tctgtatttt taatgaagga cgattttgat gaaaattatc tcctgcaagt
    36901 aaaacaaaat caacgttatt tattattgcc tgttcaatga tatttgataa aacggtcaga
    36961 ttcgtcttca atagtttttc ctgtacagtt ttatcaagag ttgtcagtcc ttcaaatgac
    37021 cgatccatat gtaaatctgc cgcatggata aatttcacca ttaaaaattt cctccttttt
    37081 ttcttttatc ataccatttt caatgttttt tttcatcaat aaccgaaaat ttgttcgtat
    37141 tatgcaaaga aattacgata aatggccgac ttcaaaaaat aagtttattc gaaaaaaaga
    37201 ttgtgacatg atttttgtca caatcctaaa tatctatgtt atattcgctt cgtttgacac
    37261 tgaagaagta ctatttgtac tggaatttat gctccatttt tccgtattta gaattctatg
    37321 ttctatacta ccttaattag tttatatggt aagaattctt tcaaatgctt gagatactcc
    37381 gtctttatca tttgaaacag tgacataatc agcttttttc atgatttctt gatgactatt
    37441 tcccattgca aaactaatat ctgcactttc aaacatagat aagtcattta aactatctcc
    37501 gatcgcgatt ctcaaaggtt tctttaaatt gtagattgat tccaaacgat caaacaccac
    37561 acctttagat acattttgag gtacgatttc aatatttaca ggtgccgatt cagtaacaga
    37621 cacatttagc atctttttca aatggttaaa tatattagct ttcaattgac gattaggaat
    37681 aaaaataaaa aatttgtaaa tgctgatagc atccttttct ataatttcct caatattatt
    37741 ataagggatt gaacaaatag atttttcata ctcaagctca tagtttagct ctttgataga
    37801 aaattcactc cctacctcgt gaaaaatttt tttcatttta tttttataat tctgagattc
    37861 ataacttccg agatttgtat atattttggt tggaaatttc gataggacag aatcaatttt
    37921 ttttacatca tttttatcga aggaagactc aaatatcttt ttttctccat caaatccaac
    37981 agaaccattt aaccctactg ctggtatcct taaaccagat tttttgagaa tattattaat
    38041 gtcttctact tctcttccag aacaaatgaa tggaaaacag tcttccggta aatgtttcat
    38101 tgttcttgca tttttttgac taatgctctg gtcagaattc aaaagagtac cgtctaaatc
    38161 tataccaatt agtttcattt catccatgtc tcctatacgt tgattataaa ttcactgctt
    38221 attgtaagtc taaatttctc acaaaacata tactaattat acctaattta agtaagtaca
    38281 aacatcattt taagaatagg aaaggacatt tatatactat tctctgtatt tttgatttat
    38341 aaataaatat gaattgaatc caaatcagct atattactat tttataataa ccaactataa
    38401 aaaacgaagt taggtgaaaa actaacttcg atattcataa aagactctat tagttcaact
    38461 tagcaaaaga tttaaa
//
LOCUS       DACDRN010000018        37645 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDRN010000018 DACDRN010000000
VERSION     DACDRN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.018"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(148..2301)
                     /locus_tag="IWJ80_001177"
     CDS             complement(148..2301)
                     /locus_tag="IWJ80_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP9460921.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2595..3443)
                     /locus_tag="IWJ80_001178"
     CDS             complement(2595..3443)
                     /locus_tag="IWJ80_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9460922.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="IWJ80_001179"
     CDS             complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="IWJ80_001179"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP9460923.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5944..6123)
                     /locus_tag="IWJ80_001180"
     CDS             complement(5944..6123)
                     /locus_tag="IWJ80_001180"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP9460924.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6170..7993)
                     /gene="cadA"
                     /locus_tag="IWJ80_001181"
     CDS             complement(6170..7993)
                     /gene="cadA"
                     /locus_tag="IWJ80_001181"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9460925.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8271..10550
                     /locus_tag="IWJ80_001182"
     CDS             8271..10550
                     /locus_tag="IWJ80_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9460926.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10643..11290)
                     /locus_tag="IWJ80_001183"
     CDS             complement(10643..11290)
                     /locus_tag="IWJ80_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460927.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11469..12188)
                     /locus_tag="IWJ80_001184"
     CDS             complement(11469..12188)
                     /locus_tag="IWJ80_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9460928.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12319..13470)
                     /locus_tag="IWJ80_001185"
     CDS             complement(12319..13470)
                     /locus_tag="IWJ80_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP9460929.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13687..14427)
                     /locus_tag="IWJ80_001186"
     CDS             complement(13687..14427)
                     /locus_tag="IWJ80_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9460930.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14431..14754)
                     /gene="rsfS"
                     /locus_tag="IWJ80_001187"
     CDS             complement(14431..14754)
                     /gene="rsfS"
                     /locus_tag="IWJ80_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP9460931.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14811..15404)
                     /locus_tag="IWJ80_001188"
     CDS             complement(14811..15404)
                     /locus_tag="IWJ80_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9460932.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15388..16035)
                     /locus_tag="IWJ80_001189"
     CDS             complement(15388..16035)
                     /locus_tag="IWJ80_001189"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP9460933.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="IWJ80_001190"
     CDS             complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="IWJ80_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP9460934.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="IWJ80_001191"
     CDS             complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="IWJ80_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP9460935.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17481..18008)
                     /locus_tag="IWJ80_001192"
     CDS             complement(17481..18008)
                     /locus_tag="IWJ80_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP9460936.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18188..18973)
                     /locus_tag="IWJ80_001193"
     CDS             complement(18188..18973)
                     /locus_tag="IWJ80_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP9460937.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18966..19835)
                     /locus_tag="IWJ80_001194"
     CDS             complement(18966..19835)
                     /locus_tag="IWJ80_001194"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP9460938.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19836..21215)
                     /locus_tag="IWJ80_001195"
     CDS             complement(19836..21215)
                     /locus_tag="IWJ80_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP9460939.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21223..21642)
                     /gene="fabZ"
                     /locus_tag="IWJ80_001196"
     CDS             complement(21223..21642)
                     /gene="fabZ"
                     /locus_tag="IWJ80_001196"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9460940.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21667..22143)
                     /gene="accB"
                     /locus_tag="IWJ80_001197"
     CDS             complement(21667..22143)
                     /gene="accB"
                     /locus_tag="IWJ80_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP9460941.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22146..23381)
                     /gene="fabF"
                     /locus_tag="IWJ80_001198"
     CDS             complement(22146..23381)
                     /gene="fabF"
                     /locus_tag="IWJ80_001198"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP9460942.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23400..24137)
                     /gene="fabG"
                     /locus_tag="IWJ80_001199"
     CDS             complement(23400..24137)
                     /gene="fabG"
                     /locus_tag="IWJ80_001199"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP9460943.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24149..25066)
                     /gene="fabD"
                     /locus_tag="IWJ80_001200"
     CDS             complement(24149..25066)
                     /gene="fabD"
                     /locus_tag="IWJ80_001200"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9460944.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25173..25397)
                     /locus_tag="IWJ80_001201"
     CDS             complement(25173..25397)
                     /locus_tag="IWJ80_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9460945.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25450..26412)
                     /locus_tag="IWJ80_001202"
     CDS             complement(25450..26412)
                     /locus_tag="IWJ80_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP9460946.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26422..26862)
                     /locus_tag="IWJ80_001203"
     CDS             complement(26422..26862)
                     /locus_tag="IWJ80_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9460947.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27168..28133
                     /locus_tag="IWJ80_001204"
     CDS             27168..28133
                     /locus_tag="IWJ80_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9460948.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28190..28420)
                     /locus_tag="IWJ80_001205"
     CDS             complement(28190..28420)
                     /locus_tag="IWJ80_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP9460949.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28506..29393)
                     /locus_tag="IWJ80_001206"
     CDS             complement(28506..29393)
                     /locus_tag="IWJ80_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP9460950.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29482..30705)
                     /locus_tag="IWJ80_001207"
     CDS             complement(29482..30705)
                     /locus_tag="IWJ80_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9460951.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30722..31801)
                     /locus_tag="IWJ80_001208"
     CDS             complement(30722..31801)
                     /locus_tag="IWJ80_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9460952.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(31932..33659)
                     /gene="ptsP"
                     /locus_tag="IWJ80_001209"
     CDS             complement(31932..33659)
                     /gene="ptsP"
                     /locus_tag="IWJ80_001209"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP9460953.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(33659..33925)
                     /locus_tag="IWJ80_001210"
     CDS             complement(33659..33925)
                     /locus_tag="IWJ80_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP9460954.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34404..36638
                     /locus_tag="IWJ80_001211"
     CDS             34404..36638
                     /locus_tag="IWJ80_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9460955.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36786..37106
                     /locus_tag="IWJ80_001212"
     CDS             36786..37106
                     /locus_tag="IWJ80_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9460956.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(37311..37535)
                     /locus_tag="IWJ80_001213"
     CDS             complement(37311..37535)
                     /locus_tag="IWJ80_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP9460957.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT        10566 a         8135 c         6539 g        12405 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaac caagaagtat ttaatatttt
     2281 tttcataaaa tctttcatca tttcctatcc ttccgcctat ttacattcat gcttcattgt
     2341 acccaaaaac ttcttaataa aaaagaaaaa atgggcggag caagaaattc tttacctaat
     2401 ttcaagataa aatcaacttt ttgtttcaca gcaatcactt aaagttacat atccaactat
     2461 ttttttcatt aaaaaataaa cttatttttg aaaattatgt tttcttaagt cgtgaaacac
     2521 tttgatggat catttatttg tggaaaacta cgttttgtta ttctaaaaaa cttatataaa
     2581 aaatagtaag gtttttattt cgaatccata aaatattctt ggattgcttc tcgtgttttt
     2641 ttcacttttt cgctaagctt ttcgttttta gccatataga aataaaaaat gatatcgatc
     2701 gtagcaaatt gggccaaaag tgaattagtt gccgcacttc gattcgtgat ttcttttggt
     2761 ctagatgttt gtacgagtac atcggctatc ttgctaagcg ggttatttcc aaattgcgtc
     2821 aacgcaatga tttcgatatt catgcttttc gccagctcgg ctaatgcgac tacatctgca
     2881 cttctacctg agtttgaaac aagccaaagc actttctttt tctcattgct gacaagttgc
     2941 gggagcaaga cgtggatatc tttttcaaaa atgattggct taccgacacg agaccatttt
     3001 tgcagaatat cttctactac taaagaagag gcgccaaccc catataaaaa aacagtctct
     3061 gccgattcca aacactctac gactttttgg atcgtttctt cttctagaat atctactgtt
     3121 tcttttatcg ctacttgtgc attgttacca agtttgtttt tgatagagta aaaggactca
     3181 tttgcttcta tatcactaaa accttcatcg attccagtct gtagttctgt tgaaatcgtg
     3241 attttgaagt ccgtcagact ttgaaaaccg atttttttgg aaaaacgtac aactgtcggc
     3301 gcactcaacc cggaagcttc tgctaattca ttagctgtca ttgtagggac cttctcaata
     3361 ttaccgataa tgtagttggc caactttttt tctgctgaag aataatcttc catcatactt
     3421 tcaatacgac taattacgct cataagttcc tccacccttt taatgatagt ctatgtaagt
     3481 ataccatatc agacccttat tcacggtcgt atactggata aaaaacaaaa aaacaatccg
     3541 cccaacagaa tatcttatcc catcaatccc acagattcta ttgagcatct gtgtctttga
     3601 ggaatacctg ttatctattg ttcggattgc tttctatcta ctactttttt acaggaaatt
     3661 tacctattag ttttctctgc tgtaattttt gacttctgct tccatagcat caataaagat
     3721 attcaaatct tctacagctg tttctttact tccataacga cgtacgttca ctgttgcatc
     3781 ttccaattcc ttgtctccaa caactaattg gtaagggatc ttttgtgttt gagaggcacg
     3841 tatcttatag cccatttttt cattacgatc atcaacttct acacgcaacc ccttcatttg
     3901 caaacgttct ttgatttcat aagcataatc gccatgtgca tcaacagaaa cagggatgat
     3961 tgttgcttgt ataggtgcca accatgttgg aaaagcgcct ttgtatactt ctgttaaata
     4021 agcgacaaaa cgttccattg tagaaacaat tccgcggtgg ataactactg gacgatgcgt
     4081 attttccccg tcttctccaa catatgtcaa atcaaagcgc tctggcaata agaagtctaa
     4141 ttgtatagtt gataatgttt cttctgtacc aagagctgtt ttgacttgaa catctagttt
     4201 tggtccgtaa aaggctgcct cgccttctgc ttcaaagtaa tctagttcta attcatccat
     4261 agcggctttc agcattgctt gggctttttc ccacattgcg tcatcatcaa aatatttatc
     4321 tgtattgttt ggatcgcgat agctcaagcg gaaacgataa tctgtaatat tgaagtcagc
     4381 atatactgcc accatcaatt caagcgtacg tttaaattca tctttgattt gatcaggacg
     4441 aacaaatgta tgaccatcat tcaacgtcat ttctcgtaca cgttgtaaac ctgacaaagc
     4501 acctgatttt tcatagcgat gcatcattcc taattcagcg atacgaattg gtaattcacg
     4561 ataagaatgg atcgtgtttt tgtagaccat catgtgatga gggcagttca ttgggcgaag
     4621 aacaagcatt tcgccatctc ccatatccat tggcgggaac atgtcttctt gataatgatc
     4681 ccagtgtcca gatgttttgt acaactcaac atctcccatt attggtgtat atacatgttg
     4741 gtaacctaga ctaacttctt tgtctacgat ataacgttcg atcgtgcgtc ggattgttgc
     4801 cccttttgga agccagaatg gcaaaccaga acctacttct ggggaaacca tgaacaagtc
     4861 aagttctttt cctagtttac gatggtctct ttcttttgct tcttcacgga gacggatata
     4921 ttctttcaaa gctttcttgt caaaaaaggc tgtcccgtat acacgttgca tcatttgatt
     4981 gtttgagttt ccacgccaat aagcacctgc cactgataat aatttaaata cttgtatacg
     5041 gccagtggat ggaacatgtg gtccacgaca caaatcgatg aattcgcctt gttgataagc
     5101 agtaatcact tcttcttctg gcaattcact aatcagttct actttgtacg gatcacttgc
     5161 aaaaatttct aatgcttcct gttttgaaag aacacggcgt tcgatcggca gattttcttt
     5221 gacgattttc atcatttctg cttcgattgc cggcaaatct tctgcagtta cttggttttg
     5281 tccattatct gtatcgtagt aaaaccctga atcgatagct ggtccaacgc caaagtggat
     5341 atttggatag aggcgacgca tggcttgggc catcaaatga gctgctgaat gacggactaa
     5401 gccaagtgca tcttcatggt ctggtgtgat gatctcaata cttgcatctt cctcgattgg
     5461 acgagtcaaa tcaacgagtt ttccgttcac tttaccagct aaagcttttt ttgctaagct
     5521 ttttgaaatg ctttcagcaa ttgccaatgt tgttgttcca gactcaaatt ctttgactgc
     5581 accatctgga aaagtaattt ttatcataat agtttcctcc ttgaaatgtt ttgattgatc
     5641 agatcatcaa gctgaaaaac aaaaaaatcc tagcatctct tgaaaagaga tactaggacg
     5701 aaaagttcgt ggttccacct aatgttcaaa cgcaaatgcg ttcctcaagc gttgtaacgg
     5761 tccgaccgcc cttgcttcaa caagggtttc gaaagtggtc atcctttggt tttctcctgg
     5821 aaaagtttca gcctagcttt tcctctctta tcgaagactc tcaaattcag ttggctttct
     5881 catcaatcaa ttatgtgttt atttaaccac taataaatag aaaaatcaag tgggaaacta
     5941 aacttatttt ttccattccc catttacacc gtatgttcct tttttcatct cattgatgat
     6001 gacatggatg tgttcttttg gtgcctgcgt gtttttatga acagcttccg tgatatcttt
     6061 catcatattt tctaattgtt ccggactgcg accttctact aattcgacat gtacgaatgg
     6121 cataatgctc tctcctcttt ctgatcgttc ttttttcaac tcgattctat catactatgt
     6181 ttgtcttggg tactgaatca ggaaaaatgg ctggatcttt ttttgctctc aaggcactca
     6241 aaatagagac cgtatccacg acttcttgca aaacagctcc gaataatgcg gggattagtc
     6301 ccgtacttgc aattaacatc aaaaaaatac aaataaagat gccaatcagt acagactgtt
     6361 tcgctattct catcgtatct cttgaaatca ctactgcttt tgctacacgc aagagatcat
     6421 ctttcaagat gaccgcatct gcactttcac tagcagctgt agaaccatga gcccccattg
     6481 caataccgat atctgcagca gcaagtgctg gtgcgtcatt cacgccgtct cctaccatcg
     6541 caacaggtct ttgatcttta gggatacttt ctaaaatggt cagtttatct tgaggcaagc
     6601 tacgggcatg tacagaatct aattggactt tttctgcaat cgtttgagca atggcagatc
     6661 catccccggt gatcatcatc aaatttttca catcaagttt tcttagctcc ttgattgtct
     6721 ttgctgcttc tgggcgaatc tgatccgaaa ataggatcgt tcctgcaaaa gatcgatcaa
     6781 ttgttacata gattgctgta ttggacgttt ctatttcttc agctgaaaca aaagaggcac
     6841 gtccaatctt tacttcatga ttattgacta ctgcttggat tccttgaccg gtcacttctt
     6901 tcagttgact aacaggatac agttggctgt ttttcacgtt gttcactaac gaacgagcta
     6961 agatatggct agattcctgt tctgcactag ctgcatacag aagtagctct tcttttttta
     7021 ttccattgac tggctgaatc gcctctactt ccaatatccc tttggtaagc gttcctgttt
     7081 tatcaaaagc aatcgttttt gtacgtgcga gcttttcaat cgttgttcca gtttttacaa
     7141 cgatcccgtt tttacttgaa cggctcattc ctgcaactaa agcaatgggc gcagctagaa
     7201 tcagtggaca aggtgatgcc actaccagta cctcagcaaa tcgtaaaggg tcttttgaga
     7261 accaccaaga aataccaccg atcaaataag caatcaacgt gaaagggact gcataacgat
     7321 cagctaaacg gacaaatcgg gcaggctttt cttttgattc ttcgactagt ttaataatca
     7381 tttgatactg gctgtctgcg gcacgttttt cagcgatcat ttggatcgat ccgtctccat
     7441 ttaaagaccc tgacataatc tgatctccag gtttcttggt ttccgggcgg gactcacctg
     7501 tcaaagaaga ttcatcgacc gaagattctc ctttggtcac tcgaccatct gcgggtacaa
     7561 gttcgccagg cttgactagt aactggtcat caatatttat ttcatcgatt actatatcct
     7621 gcaattttcc ttctttgaat cgatgggcaa ttcttggggt gttatctaac aaagactgca
     7681 gttctttacc cgctcgttta ttcgcgtaat cctctagact atctccgccc gtaagcatga
     7741 tcaatatcat caaactagcc cagtattctt tgacggctaa tgttgcaagg attgcggtaa
     7801 tcgctaatac atctacgcca tatcttcctg aacgtaacgt cttgatcatt tccaaaaaca
     7861 tcaagaaggc gagaatccct ccagtaaatg cgacaatcgc aaatgcaagg gccggctggt
     7921 gaaaaacaaa ttcacttaat aaagctatcc caccaataag caaagtaagt aaaaattttt
     7981 tgaaatgtgt cattgttttt tctcctcttt tatgtgactt agagaggtta gtaataaatt
     8041 tccagctgcc tctttcatct taagcatagt ccttgactag cactttttaa aacaataggc
     8101 cttaattgaa aacaactatc actatcagcg tttccaaaat tcatcagttc tacaaacaaa
     8161 aatattcagc aaggattact ttttcgattc atcagttaaa aagtaccttt tttcgaaaaa
     8221 ctatgctata cttttaattc gttatttacg gcggaaggag caaacatgcg atgaatgaga
     8281 aacaactaga acaaacgcac gtggatgaaa cgatcgaact aatccagatc gaacaaaata
     8341 tgctggatac aaaacaacaa gaattgactc aggaaatgaa tcattcgaca aaagacacag
     8401 ctaatcacaa gattcgagct ggttccaatg aagcttttta tgaatctgct gttgaatatc
     8461 gccaacatga gcaggaattg cttttgaaat accatactct tgaagcacag caaaaacgcc
     8521 tgcaaacgtt gtctgtcatg aaagatagtc cttattttgc ccgtatcgac tttaaagaag
     8581 accgtgaaac agaaacatta tatctaggta tcgcttcttt aagagatctg gcagaagaaa
     8641 caatcgttat tgactggcgc gcaccaattg ccaatcttta ttatgaaggt gagattggtc
     8701 ccgcatttta tgaaactgaa acagatagaa tagatgtcga tctgcagttg aaacgacaat
     8761 ttaaaatcca agaaggacgt attttatcta tggtagatac gtcggaagta atcaatgatg
     8821 actttttgct agagatattg gatgaatcat caagcaacca tatgaaaaac atcgtctcta
     8881 cgatacaaaa agcacaaaat gcaatcattc gggacacaac aagtaaagta atgctgattg
     8941 aaggaatcgc cggaagtgga aagacatccg ctttgcttca acgtattgct tttctgttgt
     9001 atcataatcg caagtggctc gaggctgaaa acgttcttct attttcaccc aaccatttgt
     9061 tttctgatta catctcgact gtattgcctt cattaggtga aagcggcgtg cctacacaga
     9121 cattcaaaaa ttatttggtg cagctgctgc cagagttttc tttactagat gaacagcaac
     9181 aagaaagtgg ttttttgcta ggggaaaaag atccgataca agtgatgaaa agcggattga
     9241 ccttagtcga tcagattgat cgctatatcc aaggcatcac aaactacggt cccttgttcc
     9301 gtgatatgaa aataaatggc cgtacgatcc tatcaaagga atcgattcgc caatggtata
     9361 aagaaacaaa tgaacaatta cctttacatc atagactttc tctattacaa acgaagctcc
     9421 tgaaaaaatt aggcggtctg caaaaagacg aaacacgtca gcaatgggtc aaagatctgg
     9481 cggaagaaca gctccaagaa ctatacgcaa cagatccaaa tcttgaatat acagaacaaa
     9541 aggagcgttc attacgtaaa aaactggcca accaaatcgt acgtaaaaga ttccgtcgaa
     9601 tccatcgagg gatcatgaat tatcaatttg tcaatataac gaaacaatat ctccactttc
     9661 tacaaactgt gccactttct cttctaacac gacataaaat cagcgagacc aggtggcagg
     9721 aacatttgac aactgttcgt acacacttgc gtcaaagaga attgcgtcaa gaggatgctg
     9781 tactattctt tttactgatg cgccgaacat atccagtgac ggttacacac aaagctcgct
     9841 atatctttat tgatgaaatg caggatttcg caccatcaca agtggcattg ctgcaagcct
     9901 tgttcccaaa agcaaatttg actttctgtg gagatctaaa tcaaaatgtc tttggaaatg
     9961 aaacgatcgt tggatcattg gaagaccttt tcgtggaccg tgaagtcact cgtttccaat
    10021 taacgacaag ctatcgctcc acaaaagaaa tcactgattt tgctaatcat tttattgtga
    10081 aagaacatca agtagaaaca acagcaagaa aaggagacct tcctttgctc atccaaggaa
    10141 caacagatga tatcaaattg aattggttag cagataccat tcgaacgact cagggaaaag
    10201 cccggtattg gcgtacagca atcatagcta aaacacaaga agaatgcgaa gctctttatg
    10261 ctcaactgcc agacgagttg aaacaacaag tccagctgat tttaaatgag agtgacttta
    10321 tgaagcgttc cattatttta cttccagcct ttttagcaaa aggtctggag tttgaccgag
    10381 ttttcttgtg ggatgtagat gcagaacatt tttcttctca gcaagacaaa ttgatccttt
    10441 acaccatgtg tacacgtgcg atgcatgaat tgaccctttt tacctccaat aatgaaagtc
    10501 ctttgttaaa caatatggat atttcatctt atgagaaaaa agtactgtaa gtaaaaagac
    10561 aactgctatc attaatgtta gcagttgtct ttttatttac aataataatt gcagaaaaag
    10621 taattcaatc atttaaggtc atttattcta tatataaatc caaatctgtt tcaggatatt
    10681 ttaatttgac aagcgaaata atcattttca gttcttccat gttttctgga tattctgggt
    10741 caaatacctt gctatgacgg ccaatcccgc cattgtcgat caatcggtaa attcgattcc
    10801 attcaatgaa ttggtcaatt gattggaaat tgctgattgg atagccgcca caatccaaaa
    10861 tctctgatac acgaaatgct aggtatgcac tatcgatcac tttatttttt ttcgtgtcta
    10921 tcttctcatt tctttcgata agaatttttt caggacgatg tttcaagctc cactctaata
    10981 gtagaaagaa agcctccatc cgatactctt cttcaaataa ccgaaaggct gaatcagaac
    11041 gacgaaaaag cagcggaatc agataatttt gtctataaat cagcattggt gcgcggtcca
    11101 ccatacactc taaataacgg ttcagatacc ctgtaccgac atagtttcct aatcgatagc
    11161 cttcaacgcc gaatcttgcg tatttttgcg ctaattttga acaatcagtt acgttcacag
    11221 ctaactcttc tactttgcgg taaccgctta agatacgttc aaaaggttct aaagtgattt
    11281 ttgcttccat attcttcccc gtccatcttc cacatacttc atatcatttc acatgatact
    11341 tatgctctca tcataccaca ggttgaatta gaaaagaaaa taaatcacac aaaaaagcaa
    11401 gacaatctgt tagtgagatt gtcttgcttt tcgaaaaaga ctgaatactt ttcgttaaaa
    11461 ctggttgctt agtcttctaa tgccacatta tggaacacat gctgtacatc ttctagatct
    11521 tccagtgcat caatcatttt ttcaaatgtt tcttgatctt ctggagatag tgttacctcg
    11581 ttttgaggga tcatttggat ctcagcgatt gaaaaatcag tgattccttt ttcttttaat
    11641 gcttcttgta tactatggaa atccgctact tcaccgtaaa cgatgatttg tccttcttct
    11701 tccattacat cgcgaacatc gatgtctttt tccattaggt attcaaggat ctcgtcagcg
    11761 tcttctcctg caaaagcaaa gattgctgta ttgtcaaaca tgtaagaaac cgctccggaa
    11821 acacccatgt tcccgccatt tttcccaaat gctgcccgta cgtctgctgc tgttcgattc
    11881 acattgttcg tcaatgtatc aacaatcacc attgagccgc ttggtccaaa tccttcatag
    11941 cgcaattcag agtatgtttc atctcctgca ccttttgctt tttcgattgc tcggtcaata
    12001 atatgttttg gtacattata agttttagcg cgatcaatga cgaaacgtag tttttggttt
    12061 gcatgcggat caggatcacc tgattttgct gccgcgtaga tttcaatccc aaattttgca
    12121 taaatccggc tattattagc atctttagca gttttctttg ctacgatatt tgcccattta
    12181 cgtcccatat tttcacttcg ctttctgatt tgatagtttc ataacgcaaa aacgtcctgt
    12241 tccattatac ttgtgaatca cgattctgtg aagctttttt tctttcttcc ttcattcttc
    12301 tcatttttag tcaattatct atactcggat tgggatttgc ccgaaattct gttccggaat
    12361 attgcctccc aattgataga tttgatcact tctaactaac agttccgctt cttgtgcctc
    12421 tttttttccg attcgggaat aaatagaaat ctcttttttt tcttttaaga atcttcttcc
    12481 agcatcggta aatccaagta gtcgaagata atttttattt tgttggacag gaatctcttg
    12541 ctttttgata ttcagcaaga cataacaaga taatcgttgg atccgtgtcc atgtatatct
    12601 ttttgttttt acctttgaca caaaatcctc aaaactatca gcagttgcag ctgctgtttt
    12661 catgcgcgat tctattcctt cggtcatctg atagatatct gtcagttcca gcaaagtaga
    12721 agataacaac cgataacgca aatacggcca ataagattcc caagtctgca gcgaacaagt
    12781 tgaaagatct tcttctgtct gaggcggcac aagtccagct attctttttc cttcttctaa
    12841 cgcatggcga atggctgttg cactggctat cctctgatct gtgatgattt tttcatgata
    12901 gcctgcactt ttacgtcgga tcggatgtaa ttccataggg tggacatact tggcattttc
    12961 ctttgcataa cttaatccca agatatgatt tggcgaagaa aaatcaagcg taatatcggg
    13021 atacagactg cgaaaaacct cagtcatttt ttgcggataa ctatacgttt gatcagacag
    13081 ttcttgaaat gcctcttcta tcctatcttg attctcttgg acaaagcgtc cgaaactgct
    13141 gtaatcaaac gaagtctctt cttctgttcc aaaacacaaa ctgtcacagt ccagtgtttg
    13201 caagattttt actgcacctt tagcaaaata gtcagctgac tgaaccgccc attcgacggg
    13261 aagctccact actaaatcca caccattttg taaagcttca ttcgcgcgat gccatttatc
    13321 gatgatagcc ggttcgcctc tttgcaaaaa attcccgctc attacagcaa tgacgacctc
    13381 tgctcctgtt atttttcgtg ccatttccac atgatagcga tgtccattgt gaaaaggatt
    13441 gtactctacg ataatcccgc agcttttcat gcgcatcctc cttcatagtt atcgataaaa
    13501 aagaaataga gacaagtaaa aaaaggcaaa aatcggaaaa aatacccgaa ctatataaac
    13561 tattctcgca aacacaatac ttctgcgacg aaaagctgca ctgcaagagc aatgcctata
    13621 tcgttcggat atataattta tgttgaagat ttattctttt cttgttaact cgagcaggca
    13681 aaaccgctag tctttgtatg ctacaaagaa ccagcgcttt gatttctctg atgctgcttc
    13741 gtctgtaaaa tcagaaaatg cttcgacttt cgaaaagccg ctgttttcca gcattcttaa
    13801 ataactttcc aatggatagg tacgctcttg atgtagttca tcttcacgaa taaatcgacc
    13861 agacccttct tgttcttcaa cgaaaaaggt aagaaaatgt tcgatgctgt gtttctcgct
    13921 tcctggataa ctatcccaca aaaaagcaaa attatcccct tgataatgat agctatattc
    13981 tggaaaaacc tcatctactt gataggtgga gtgtacgtca aataaaaatc ggccattttc
    14041 ttctaacgct tgatagactt catcaaacac ttgctgtacc tcttgtctat ttttcatata
    14101 gcacagcgaa tcagaaaaac aggtaattgc ctgatattgt ccgatttctg ataaatcgag
    14161 catattgcct tccacaaatt ggatcgatgc gtcttcttca tatgctcgct ggctggcaat
    14221 catcaacatc tcttctgaaa ggtctaaccc tgtcacttta tatccatctt ttgcaaaggc
    14281 aaccgctaat gctccagtac cgcatgccag ttccaaaata ttttttgtgt cttcttgtaa
    14341 atgacgtttc gaaaaagcta accattgctg ataaagtgtt tcgtccatca cttcatcgta
    14401 aatgaaagca aatgtttcat aagccatatg ttaatccaac cattcactaa gatcgactag
    14461 cggagcatct gaccatagtt tttccagatt atagaaattt cgttctggcg catggaaaac
    14521 atgtacgatc acatctccaa gatcgatcaa tacccatttt ccgccttctt ttccttcaat
    14581 acgttttact tcatatttgt tttcttcttc tttatcaatg atttcttctg taattgcgtt
    14641 gatttgacgt tcgctatttg ctgaacagat catgaaataa tctgccaaca aggaaacatt
    14701 tcgaacgtct aaagcgataa tgtcttctgc ccgttttgaa tctgctgctt caatcgcaat
    14761 ttttaattgt tctttactat ctatgatgat ttcctcctaa tatgagcagc ttattttttt
    14821 gctacccagt aattatatgt ttcgattgtt tttggataaa tctgttgttc ttgcgcaagc
    14881 aaatgggcca atgtatgttt tgtttcaaat gctacagctt cgtctagatc agcccatgca
    14941 atggctcgtg catcttgtac cccaggaaaa tctcgacctg gttcaatata gtctgctaca
    15001 taaatgattt tgtcaaggag actcatctta gcagccccag tcgtatgcac ccggattgca
    15061 tgaagaatct ctgcatcggt gattcctaat tcccgctcta caaacgaagc gccaacaaga
    15121 ccatgccaga ttgcattatt ataattcaat aactccggat caaaaccgtc tcttacgatt
    15181 accattttga actcctcatc tggtcgttct tttgcataat catgtgtcaa tgcagcaata
    15241 ctagcttttt caggagaagc accgtatttt ttggcaagag ccacggctgt ttcttctact
    15301 ccaagcacat gcttgaagcg ttgttcgctc attctcatct gtattttttg catcaggttt
    15361 tctctagaat aagcagtata ttcattacta tagtccatat tcatacaacc cttcattttg
    15421 aatataatca attactttat ctggaacaag gtagcggata gaacagcctt ctttgatctt
    15481 ttgacgtatt ttcgtggaac tgatgtcgat ctcaggtaca tctacccaaa ttacaggata
    15541 tggagtatcc gttgtgtatc ctggtcgacg tatacccaca aagttgacca tagaagtcag
    15601 ctcatcgatt ttataccatt ttggcaaata ttctaccata tccccaccga taataaagta
    15661 ataatctgta tccggattgt tttgcgtcaa ttccttcata gtatcatagg tgtaactttt
    15721 cccaccacga gctaattcaa tcgtttcaat ctgcaaaaaa ggattgtctt ccaccgctaa
    15781 ttccaacatg ttgagtcgat gttttgcatc aatcgtctgt ttctcatcaa catgaggtgg
    15841 ctgataagaa ggcatcaaaa atacgcggtc aagacctaaa ttccggccag cttgttcagc
    15901 catcactaaa tgtgcaaggt gaacgggatt gaatgttccg cccaagatcc ctacttgctt
    15961 tttcttctct aagaattgag gcatttcttc aggaaataat tgtgaacgaa caaatgtctt
    16021 agcttgcgta ttcatcatgg tcactcctta tattgctttt acttcttttg atagcctttg
    16081 atacttttct tttgtggaag gtttgtataa tacaataaca cgtccaatga tttggacgat
    16141 ttcgcaatct atttcttttt ctaatacttg agctgcttct tctgcaatct catctgtatt
    16201 ttgaagtaac gatactttga tcagttctct tttttctaaa gcttcttcaa tttgaacgat
    16261 cattgctgaa ttgatcccac cttttccgat ttgaaaaatc ggctgaagat gatgcgcttt
    16321 gctacgtaaa aaacgctttt gttttcctct taaactcata attttccttc ttccttaaat
    16381 caatgctttg cgtttcaata catctacacc ctttggcgcc cagcccgcaa ttactactgg
    16441 cgctgttaca gtgatccagc ctagaccggc aaaaacgatg tctgtttttt ctttcacaga
    16501 aaactcaaaa cgaacaagct ccggaaatgc ctcgacttca tcaggacgag gaggctgtaa
    16561 taatccacct acatgttttt gataaaattc gtctgctttt tccagtttcg ttcgatgaat
    16621 atttacatca tttgacacat aagcaataaa tgaagcacga ttgccgctga taaaatcaaa
    16681 tcttgcaagt ccgcccaaga aaagtgtttg cccttcattc agctgataaa ctttaggttt
    16741 gatttctttt aacggtgcaa tgatccgcaa atctttttta cctaagtagt gcgccatttg
    16801 atggcgatgg ataattcccg gtgtatcaat caaaaaatgc ccgtcatcta aaggaatctc
    16861 gattttatcc aaggtcgttc ctggaaactg cgaagttgtg attacatctt gtactcccgc
    16921 tgtatttttg atgatttgat tgataagcgt cgattttccg acatttgtta ctccgacgat
    16981 atagacatcc cgtccatcgc ggtattcttc gattttttct aatagttccg tcatctcatg
    17041 agatttctta gcactagtta ataatacatc tactggacga attcctgctt cgtaagcacg
    17101 ttccttcatc cattgtgtta atttagtttt tttcaatgat ttaggtaata tatccacttt
    17161 gttcccaact aaaagtactg gattgtcacc gacaaaacgg tgtagtcctg ggattagtga
    17221 accattgaag tcaaaaatat ccaccacatt gacgatcaat gcatcttttt gccctaattc
    17281 attcagtaag cgcaaaaagt cgtcatccgt caatgagaca tcttggatct cattataatg
    17341 gcgtaatctg aaacaacgct gacaatagac ttccccggaa gctaaacctt tttccaaagc
    17401 cgtttgcggc gtgtatccta attcaccggg tttgtttgtt tgtataacgg caccacagcc
    17461 gatacattgt aattcttctg tcattcgatt ccacctcgcc agcccatatc agggtgtttt
    17521 gctaatagat aggccataat tcttttttcc caaaaacgat taaattgtgt cttccaacta
    17581 tctgttgcga taatgggttt tacaaggatg ctacgtatcc ctgcacgatt ggctccacgt
    17641 atatcagtca taatctgatc gcctaccatt actatctcat catctgctaa atccaattgt
    17701 ttctttgcta tgttgatccc tcttgctaaa ggtttcattg ctcgtgcaac atacaaaaga
    17761 tcaaattttt caatcgctcg cgcgacacgt tttgaattat tgtttgaaac aacaacaaca
    17821 gggattcctg catttttcat ttctaaaatc caagtaagca actcttctgt tccatctgga
    17881 ttattccatg caatcaacgt attgtctaaa tcggtcaata ctgctttgat gcctagattc
    17941 tttaattggg caggtgtaat ctgatagatg gcttctacca tccatgttgg tttgtaattt
    18001 gaaaacatgc tgtctcctca tctgcaaaaa atgaaggagc acataaagta ctcctcaacc
    18061 ccgtattata ctgcatatca gcaaaaaaaa ctacctctat actcgttcgt ctttcagaaa
    18121 aagcatttct ttacaaaatg accaaaaata aagattgatc acagaaaaat ttagaaacaa
    18181 aaaacaacta atattggcgg aacctcttgt atcgtttttc cagtaattta tgctgatcta
    18241 atcgaacaag ttccgtcaat ctttcaatca acgctttttt cataatgtgt gcgactttat
    18301 atcgtggcaa ctgctcgccg ttgaacgttt caggaataac tcgatcaacg attcctaact
    18361 cgcgtagttc tggcgcagta attttcatca actcagctgc ttctgcggcg cgactaccgt
    18421 ctttccacaa aatcgacgca aatccttctg gagaaagcac agcataaatc gtttcttcca
    18481 gcatccagac ttcatccgct aaacctagag caagggcacc gccacttcca ccttcaccaa
    18541 tgatgatggc gatgatcggt acctttaaat cagacatttc gagcagattt ctggcaatag
    18601 cctctccttc gccgcgctct tctgctccta caccgcaata tgcacctgct gtattgatca
    18661 aggtaatcac cggtctattg aacttttccg cttgtttcat cagtcgtaaa gcttttcgat
    18721 agccttcagg atggggagaa ccaaaatttc tgcggatatt ttcctgcaga tcccgtcctt
    18781 tttgaatacc gatcactgtt accggtttgc catctaatcg agcgattcca ccaacaatcg
    18841 cttcgtcatc tgcaaataaa cgatcaccat ggcattcctc gaaagaatcg aagatctctt
    18901 caaaaaaatc caagcttgtc catcgttctt tagctcttgc taactgaacg acatcatgtg
    18961 ctgtcttatc cacgatctat ccatcctttc tgtgtatgca attcaagaag cgtatggatt
    19021 ttctgtttca attccatgcg gggaacaatc tgatcgacaa aaccatgaga taataagaat
    19081 tcagcctttt ggaaatcttc tggaagttcc tgcttgatcg tctgttcgat gacgcgtctt
    19141 ccagcaaaac cgatcaagct ttggggttct gctagaataa tatcgccttc cattgcaaaa
    19201 ctggcagtta ctcctccggt cgtcgggtcc gttaaaacag ttagataaaa aagaccttca
    19261 ttgctatgcc gtttgacagc agcagagatt tttgccatct gcattagaga aaagattcct
    19321 tcttgcatcc gtgcgccacc tgaagctgtg aacaagacga ctggaagact ttcctttgtt
    19381 gctcgttcga ataatctggt gatcttttct ccgacaatcg ttcccatact tcccatgata
    19441 aaattaggat ccatcactcc aataatgaag ggaatcccct gaatggttgc tttaccagtg
    19501 aggactgctt catgaagtcc tgttttatct tgcatttttt taattttttc cgtatattcg
    19561 ggaaatgcta gcggatcttt ggttacaaga tccgtatccc attcttcaaa acttttttca
    19621 tcaataatca aagctagacg ctgccatgca ccgattcgaa aagcgtatcc gcaatgaggg
    19681 catacttttt cttcaccgat gtctttcgta taaaggatat gtttgcaatt tggacacttt
    19741 gcccacatgt tatcaggaac agatggcgag ttagcaggtt tgctctctct attaggattg
    19801 atccgaatat atttcttttt cttaaacaga gccatctatg cgcctcctat ctccgtttct
    19861 ggtgtccaat tcggtaaaaa ttcttcttgt aaaaatgctg tgctgtaatc acctgctatg
    19921 actgcaggat gactaatcaa atccatctga aattctgcgt tcgtgatgat tccttctgtg
    19981 ataaattcac ttaaggctcg ctgcatcttc attaatgctt caaaacgcgt atttccatgg
    20041 acgattattt ttgcaatcat tgaatcataa tatggcggga tcgtatatcc cgggtaaact
    20101 gcactgtcta ctcgcagtcc catcccacct gctggaagca gtaagttttg gatcttccct
    20161 ggagaaggag caaaatggaa agcaggattt tctgcattaa tccggcattc gattgcgtgt
    20221 ccttggaata ctacttcttc ttgacacaca ttcaaaggct caccggctgc tatctccacc
    20281 tgttttttta ctaaatcgat tcctgtgacc atttctgtta ctggatgttc cacttggatt
    20341 cgagtattca tttccatgaa ataaaaatcg ccttcttcat ccatcagaaa ctcgatcgtt
    20401 ccggcatttt cataattgac tgctttagct gctcgaacag cagcgtctcc tagtgcttct
    20461 ctttttgttt gtgagatcac tacagaagga gattcttcca gtactttttg attgttgcgt
    20521 tgtaaagaac aatctcgctc gcctaaatga atgacatgac caaatcgatc acctaagatc
    20581 tgaacttcta tatggcgagc aggataaatg attttttcca aatacatatc atcatttcca
    20641 aatgccgctg ctgcttcttg ctgagctgaa gaaaaatgct gtgggagctc ttcttttgat
    20701 tgaactttcc gaatgccttt cccgccacca cctgcagctg ctttcagcat cactggataa
    20761 ccaattctgt ctgctatttc caatgcttct tctactgtag ataatacacc agtacttcct
    20821 ggaatgactg ggacatttgc ttcttgcatc aattgacgcg cattgatctt atttcccatt
    20881 gcatcgatcg ttgcactttt aggaccaata aatgtgatat tgcactcttc acacatggag
    20941 gcaaatcggc tattttcaga taaaaaacca aatcctggat gaattgcttc ggcttttgtc
    21001 acgattgcag cgctcagtac ttcctgaaca ttcagatatg aatcaacggc ttttgccggg
    21061 ccaatgcaga ttgcttcatc tgctaactgt gtatgaagtg cttcggcatc tgcttctgaa
    21121 tatatcgcta cggtttggat ccctagttcg cggcatgcac gaatgatacg tacagcgatt
    21181 tcaccgcggt tagctatcaa tactttagaa aacatatttc acctatccaa tcataaaggt
    21241 taattccgct tcggctactt ttttaccgtc tactttagcg attcctttgc ctattccggc
    21301 tgaagctttc actttcaata tctcaacttc aaggtataag gtatctcccg gtgtaacttt
    21361 cttacggaat tttgctttgt ccaatccgcc gaaataagcc gtctttccct tgaattgttc
    21421 aagagaaagt aatgcaactg ctcctgcttg agccattgct tcaacaatca atacccctgg
    21481 cataacaggt tcttctggaa aatgtccttg aaaaaagggc tcattgatcg ttacattttt
    21541 cttagcaacg atccgttctc cttcaaccat ttcttccact cgatcaatca aaagcattgg
    21601 ataacgatgt ggaatgattt cttttatttc ttgtatgttc attgttttcc ctctttcctg
    21661 ctttgcttat ttttgctgaa tcctaaagag tggctgtcca tattcgacaa ctgactcgtt
    21721 ttctacaagg atttctgtga tcactccgtc ggcctcacta gttatctcat tcatcagttt
    21781 catcgcttca ataatgcaga taacttctcc tttggcaact tgatcaccta cttttttgaa
    21841 agctggtttg tcgggcgctg gttgcagata aacgattccc acgattggcg aaacaacctc
    21901 gattccatca aatactgccg attctttttc tttcattatt tctggagcag ttgttgcttg
    21961 ttcagatatg acattgactg gctccgaaac tggcaacgtt tcgtttgttt gaaccatatt
    22021 catgcctcga cttgttttat ttttattcat atacaattca aattgacctt cacgcaaatc
    22081 gaattccgtt agcgaggatt gatcaaattg actgactaat tctctgattt cattgatatc
    22141 caaaattatt cctcccagcg tttcatgcac agaaccgcat tatgtccgcc aaaccctaat
    22201 gaattactca ttgcataagt aaaacttgca ggtttgcttt cgtttttgac aactgtcaag
    22261 tcaatctcag gatcttgttt ttctacattg atcgtaggtg ggataaattg atgttctaat
    22321 gccagcaaag ttgccaaggc ttcgatcccg ccggctgctc ccaataaatg tccagtcatc
    22381 gatttcgtgc tgctgacgta agtatctctg aaattttcac ctaatgcgat ttggattgct
    22441 tttgcttctg cctggtcatt ggctggtgta cttgttccgt gagcattgat atatccgact
    22501 tgttccggct gtacaccagc ttcttccatc gcaagcttca ttgctttaga tgcaccagaa
    22561 ccatctggag taggagctgt catatggtaa gcgtcacagt ttgcaccgta tccaacgatt
    22621 tctccataga ttttggcacc gcgagcttgt gcatgttcta aagattcaag aactagaacg
    22681 cctgctcctt cacccatcac aaaaccattc cgattttcgt caaacggaat cgaaccacga
    22741 ttaggatcag ttgattctga gagtgctgtt aaagctgcaa atccagcaat ccccatctca
    22801 caaatcgttg cttctgttcc tccagcaaga atgacatcag aatagccatg tttgatgtta
    22861 cggaatgctt ccccaattga attcgttccg ctagcacaag ctgttacgat agctgtacaa
    22921 ataccttttg cacctacacg caaagcaata tttccagctg ccatgttggc aatcgccatt
    22981 ggtacgaaca ttggagaaac tcgttttgca cctttttcat gtagacgaat aacttggttt
    23041 tcgattgttg gaagaccacc gatcccagat ccgatgatga cgccaaaccg gtcttgattc
    23101 attttttctt tatctaaacc gctcatctcc attgcttcta aagcagcgta gattccatag
    23161 atcgaaaaca tatccattcg tttgctatct tttttaataa aatatttatc gtaagggaaa
    23221 tctttgactt cacccgctaa ggtaatccct gtttctgaag catcaaattt agtgatcggt
    23281 gcgatgccat tcgtcccctc ttttaagcta ttcaaaaagc tgtctgcgtc atttccaata
    23341 ggtgaagtga ctccatagcc agtaattacc actcgattca tttgtttgct ctccttttct
    23401 tatccgtgca tgaccaatcc gccatcgaca ttgatgactt gtccggtgat atatggattt
    23461 tgtgctaaga aaactgctgt ttgtgcaata tcttctactt gtccgaaacg ctgcatcgga
    23521 atttgctttt ccgcttgttc cttgactttg tctgctaaaa cttccgtcat atcagttgtg
    23581 atgaatcctg gcgcaatggc attacatgta atgcctctag ttgcagcttc tcgtgccaca
    23641 gattttgtta atcctactac gccagcttta ctagctgcat aatttgcttg accaatattt
    23701 ccaatcaacc cagatacact agagagatta atgatggccc cttctcgctg cttcatcatt
    23761 tttttcaata cgtgctgcgt catattgaag gttccaatca aattgatatc cagacatttt
    23821 ttgaaatctt ctgcatccat tcgcatcaca agtttgtcat ttgtgattcc ggcattgttt
    23881 actaacacat ggatcgatcc aagcttttct tctgcttctt tgatcatttg tcccgctttc
    23941 tcataatcag agatgtcacc ggaaactcct acgcatttta caccgaatgc ttcgatttct
    24001 tctatctttt cttttggaat ctctccgcga ccattcaaaa tgatatttgc tccagctttt
    24061 gcaaaagcta aggccatcgc tttgccgatt cctcttgtac tacctgtgat aaatacattt
    24121 tttcctttta catccataac gttcctcctt atagtattgc tattgtttca tcaaatgtct
    24181 gcttattctc aacacgcaga actggtattg ttttatcgat ttttttcata aatccgctta
    24241 atacttttcc ggggccgact tcgatgactt gttcgatgcc gattgttttt aacttatgga
    24301 tactctcata gaaacgaaca ggttgcatga cttgctgttc aagtagtccc gcgattgttt
    24361 ctttcttcat tatttccgtt gttgtgttgc ttatgatagg aaaagaaggt tcagagaatt
    24421 gaatctgact taggtccttc gccagttttt ttgctgccgg ttctaaaata gctgtatgaa
    24481 aaggcccgct gacatttaga gggatcattc ttttgaaccc tttttctttt aatagtaaaa
    24541 cagcttcgtc tactgctttt tcttcgccac caatcacgat ctgctgaggt gtattataat
    24601 ttgcaggaga tacgatgccg tacttgctag cttcatgaca gctttcctcg atcgtttcaa
    24661 tcggcgcatt catcactgca accattttcc cgcttccagc tggggtagct tctgtcatat
    24721 aagcgcctct ttttgctact aacgcaactg cttcagtgaa ggatagcgca ttactagcta
    24781 ctaatgccga atattctcct aaactcagtc ctaaagctgc atctgggatg attccatgct
    24841 ccttcaataa gcgataatac gcgatgctga ctgttagaat agctggctgt gtatattgcg
    24901 tctgatccaa acgttcattt tctgtaaagc aaagttcagc catatcgtag ccgagaatct
    24961 cacttgcttc atcaaatgtc tgctttacaa cggcttcttc atataaatct tttcccatac
    25021 cttgatattg tgcaccttgg ccactaaata agaatgctgt tttcatgttt ttctccaata
    25081 tatccagaat atatgtttga ttcttcctat tcaaaaaggc cgagacattc ctatttcagg
    25141 aaatgtccca gcccccaacc taattttaat gattatgcag tttgtttttc tacgtaagtc
    25201 actaaatctt gaaccgtttt gattccgtct tctgtttcga ttttcacgtc aaattcatct
    25261 tcaatttcat tgatgatttg gaataaatcc aagctgtcag cttctaagtc ttcttggatg
    25321 tcagtagtca atttgatttc ttcagtttct tttcctaatt cttctgcgat gatttcttga
    25381 attttttcga ataccataat aatatattcc tccgatattg tttgcttttt atttttataa
    25441 tttaacagtc tacaactcca tgagaatact tccccatgtt aaacctccgc catagcctgt
    25501 aaatattact ttctgctgtt ttcctaaggt gatctttcca ctagaaactg cttcatccaa
    25561 taagataggg atgcttgcag cagatgtatt cccatattta tccatattgg tcaggaactt
    25621 ttcttgcggc atctttactt ttcgggcaat tttctcaata attctgaggt ttgcctgatg
    25681 caaaagcaga taatcaactg gtttgtcttt catgacttct tggatattgt ttgcaacgtc
    25741 tcgtacggca aaatcaaaaa tatctctgcc agtcatttgc aaataatacg aaccttctga
    25801 atcttctgaa tagaacggat ttttattttg ctggtagcca gcggtcaatg agctacttct
    25861 ggtaccgtca gctgctaatt tttctttcag caattttttc tgtggattcg cttctaagag
    25921 aatcccacct gctccatcgc caaacaacac agcagtcgta cgatcagacc aatcgattag
    25981 cttagatagt gtttcaccac caatcaccag tcccttagtt gagccgcagc ggatcatttt
    26041 ttcagccatg cttaacgcat aaacaaatcc tgagcacgca gcactgatat caaaagcgat
    26101 agcatgagtt gctcccaatt tcccttgtac ttgtgctgct acagatggtg tcgtgtaatc
    26161 gggcgacatt gttgctacta aaataaaatc caaatcttta gggtttagat ttttctgttt
    26221 tatcaatttt tccgccacta aaatgcataa atcagaagtc tcctgatttg ttacacatct
    26281 tcgttcacga atacccgtcc ttgacgaaat ccattcatca gaggtatcca tcatcttgga
    26341 aagatcatta ttagtaataa tcttttcagg aaggacgctg gctgttgcag taatcattcc
    26401 aaattgttcc attaaatatc ctcacttata gtcttgcaag aagtcgtgca agtttttcag
    26461 tgcatccagc aaggcatgct gttcctccgt agccattccg tctaagatgt tcttgaccat
    26521 ttcacgatga aaatgctgat ggacccgaaa tagtaacttt ccttttttcg ttagtcccaa
    26581 ctttacaacg cggcggtcat cttcactgcg gatacgttcg acatagcctt tttttactaa
    26641 cttgttaata gcagtggtca aggtcccaac agtaattgat agttctttgg ctacttctga
    26701 agttgttttt ttcttgtaca tcccgattgc ctcgatcgtg tgcatctctg tgattgacag
    26761 atctttgaat tgagattttt tcagctcaga ctcttcgatt gtcaaaatat cattgaagac
    26821 actaacaaga taatcattta ctgtttttaa atctggttcc atgttccacc gtccactaat
    26881 tagtttgatt atcaaatctt ttgatgttca aactatatct taactttttt caaaatgcaa
    26941 ccttaaaaga tcattttttt actatatgag ctttttttaa gattttactc atttaaaaca
    27001 actgataaat cagcatttat aatttctttt attagaaaaa tcatctatct cacatatttt
    27061 gattatcaaa gtattttctg tgaacagaac tatagaattc cctaaaactt gtccattatt
    27121 tcaaaatttg ttatagtctt tttattcaaa gcgtaaaagg agatcctatg ttaaaagaaa
    27181 aaatgaaaac tctccccatg ctgccgggtg tctatcttat gaaagaccga gatggtaagg
    27241 tcatctatgt tggtaaagca aaacggctca aaaatagagt aagcagctat tttcatcaga
    27301 ataaacagca ttcaaaaaaa gtattacgta tgattcatca tataacagat tttgatttcg
    27361 tcgtagtaga cacggaatta gatgctttat tgctcgagtg tcaattgatc caacattacc
    27421 gtccttttta taaccgacaa atgaattatt tcagtaatta taattatgta catattacca
    27481 ataagggatt tgtattaaca gatactccta cggctcgaac atacgggcct tttcgtctct
    27541 ataaaaaaat gccgtctatt ttgcgtataa tggaagaaac ttatcaaatg ccttggttgt
    27601 ctgaaattag tttgttagct ttgcgagtcc agcttcctga cctacaggaa atgacttttg
    27661 aacagaaaaa aaaggaactc caagggctgt ttcaaggccg aaataaaaag ctactgactt
    27721 acttgaaaaa aagacaacaa catttcatct atcagttgaa tttcgaaaaa gccggtatgc
    27781 ttcaaaaaga tatcgaatta gttacttatt ttattagacg gatccaggaa caaaagcagt
    27841 ttttaagaac accatcgctc accttcagca tgccattagc agcagatgag agtcagaaaa
    27901 agcactacct cgtttgttat ggtcaacttg cagaaacaat gattacctct ggtgacgttt
    27961 ctcctgactt ctattatgaa aagaaagaag ctcatttatc tttgaaacgt caattgtcaa
    28021 aagaagaaat cgatcctgta caaatcttga tcagttaccg caaaaaatta gaaaaagaac
    28081 agtttgaaat tgaactgtta aacaaaaaag aagctgaaaa gcaactaaat tagttgcttc
    28141 tcggcttctt ttaggcatct tcaacttcaa atgtcaatta tcctaaaatt tattggatta
    28201 agtcatagat ttccatcgct acgatatcga tattatcaaa ttcgtattgc tgatgagtat
    28261 ctgtttcagt taattcaaat gtatcggtcg ctttatcata agttactgta cacttctcta
    28321 cgccttcttt ttcaaaacgg cgaacttgca gttcattatc tgctgactcg accatagcct
    28381 ccagtcgttt tatgattgcg actaattgtg aatgtgtcat cggaggtcct ccttttctta
    28441 tttctgttat attgtatcaa aagcatagcc tacttgcatt gattttctct aaaaaaactc
    28501 gttttttatg attttttttc tggtattttt ccttcaggcc accaaagttt gatcaaagct
    28561 tgggttccgt cattctcaaa acctttatct gccaactctt catatagttt caatgcttgg
    28621 gtagttgctg gcaatacaag atccattttt tctgcttctt ctaatgcaat ctttaaatct
    28681 ttgataaaat gtttgacaaa aaaaccaggt gtgtaatctt cttttaaaat acgaggagaa
    28741 taattgctta aagaccagtt agctgcactt ccacctgaaa gcgtctcaat cactttcttt
    28801 aaatcaagtc cgtttttttg tgcatagaca agcatttccg tcattcctgt catcgtacct
    28861 gcaatcatga tctgattagc catttttgta tgctgacctt tcccagcaga accatgcaat
    28921 gtaaatgtcg taccaaattc tttgaataaa ggcagtactt gatcataaac ggcttcttct
    28981 ccaccgacca tgatcgtcag tgtaccattc tttgctccta agtcaccacc ggataccggt
    29041 gcatctaacg catctgcgcc tttttctttt gctgtttttg caattttttc tgccaggctt
    29101 ggtgtacttg tagtcaaatc aactaaaata tgtcctgaga tatctgcaga aaaaatcccc
    29161 gactgtccat agtagatttc ttctacatct ttggggtaac ctaccattgt aaagagaata
    29221 ttactttgtt ctgctaccgc ttttggtgta tctgcccaga tagctccttc tgttactaaa
    29281 ggatcagctt ttgcttttgt ccgattatag acagctactt cgtgtcctgc ttctagtaga
    29341 tggcgtgcga cagcacttcc catgacgcct gttccgataa aaccaagttt cattttttcg
    29401 acctcctaaa aatcaaaaat ccttgcttct atgctagtat acgctagaaa caaggatttt
    29461 ttcataaaaa atgtttgaat tttatctttt aaatgaattt aattttattt tgattcgttc
    29521 aatcgagtct tcttcttctt ttcttcgcat ctcttcttca tgataaacaa ccatgtcttg
    29581 ataaaattcg atcatagctt cgccgaaatg tgtagatgaa atatcataaa gtttagcttc
    29641 taaaactttt ggatctttag gaacttcact ggaaatataa tcaataagtg ctggtgcaaa
    29701 gtcttcgtcg gtttcaaaag ttttccctaa tgaaggatga tcgaacaatc ggttcaaata
    29761 agcatttcct tccacgacac aaggtgttcc ggctgccatc gcttctgtat aagtaagccc
    29821 ttgtgtttct gaagtagaag cactgacaaa ataatctgcc gctttataat aaagtgctac
    29881 atcattgttg tccacttctc ctgtgaactg cacaaattct tctaactgca gttctgagat
    29941 caattcctct aactcttcta cataaggtcc ttttcctacg ataactaatc tggcgttagg
    30001 ataagcggag atgacctgag gaaacccatt aatcaaggct tggatatttt tctcataaga
    30061 aatcctgctt aaagaaagaa tcatcaaatg gttttcctga agacctagtg actcccttaa
    30121 tttgctgata tcctgctgag tgatatcaga acgtttgaat ttttcaatgt cgataccagt
    30181 aggaatcacg cgaagcggtg ctattacacc atagctgcgt aatgtatcaa taacacgttc
    30241 acttggacaa actacacctg tcgtatgatt tgcaaataat ctagacaagt attttacatg
    30301 ggattgcctg atcacttttc ctttaccgat ataatgaagg tagtcttcat acatcgtatg
    30361 gtaagtatgg atcagaggta ttttcaactt cttagctacc atcttcccaa gtatccctgc
    30421 tccgaattct gtatgggtat gaataagatc tagttctagt tctttggcaa ccaaataagc
    30481 ataccacatg cctctaacaa caactcttcg atccttgaaa gaaataaatg gtacacttgg
    30541 cattcgaatc acatcttttt ccaacttatc cgcattagga tcggtcgttg taaaaatata
    30601 gacttcatgc ccatgtcttt ctaattcttg ttttaatgtt ttgatcgatg tagcaactcc
    30661 gctgacttgc ggaaagtagg tatcggtaaa aaagccgatt ttcatgtttt tcgcctccaa
    30721 actagtcctt gacctgattt acttttttta atttgctaga gactttttca ttcagtacac
    30781 tttcgtatac atttttaagt tcttcaccga ttttattgat acttcgctct tttgctgttt
    30841 gataaccggc ttgtgtcgta ctcggtagtt cttgattggt aatctgttgg atcagtcgga
    30901 taaattcttc gtttgtcttc cccatatagc agttcttgct atcttctaac cagccattat
    30961 acactggaat atctcgaaca agtacgtttt gtttgcttgc taaagcttct aaaacaacga
    31021 tcccttctgt ctcttcataa gaagggaaaa agaatagatc tgcacctgaa tacgcacctt
    31081 ctatgatctc tccacgaatg taaccaggaa acaggacatt ttcaggatga tcttctttta
    31141 caattttccg gatgttccga ggaatcgagt acatgggaac atgaccaaac caaataaatg
    31201 tgtattcggg catcttctta gcaatttcta cgaaatcaat gattccttta cgttcgaaaa
    31261 acaaaccgac acaaatgatt actttttgat ccggagacag tttgaaatat tctctgaact
    31321 tttgttcttt cattggatca gggtgatatt tctctaagtc gatcccattg gaaatagctt
    31381 gaatcggcac acggatacca tagctttcca acaactgttt tgaataaggt gttggcgtga
    31441 tcagatagtc cgctttttgg tacaatccta ccagatattt ttttacgata ggagaaagct
    31501 ggttcgatcc gataaaggaa tttcggaaat cttcctctgt agaatgcgca tgatagacaa
    31561 ctttttttcc ttctcttctg gctttattga ccatattatg actattgatt ccgtatgtat
    31621 tgatgtgcag gatgtcataa tcttcttttt catctagtgt ataagagata ccgacttccg
    31681 tcagagcacg tttttgatga tcaagcgcac gcccgattcc agattttgca agcatctttt
    31741 cactttcaaa atataataat accttcacct tacgtccccc ttcacttata tagtataaca
    31801 tagcctacaa actgtcactc aaacttaagc cctatttttg tctattctta ctgacaaaaa
    31861 cgttttctta aaaaagaaca aaaaaactgg cgtagctctt ttacaagcca caccagcttt
    31921 tgatacgttg tttattttac atattcatga acaagttcaa cgacttcttc catcatatcg
    31981 cattctttca atgcacgatc agcaagttca gccattttcg ctgtgtccaa gcgtttcatt
    32041 aaactacgcg ttttaagaat agatgtcgca ctcattgaga actcatctag acccatacct
    32101 actagcaatg gaacagctgt ttgatctcca gccatttctc cacacatacc tgcccatttt
    32161 ccttcagcgt gtgctgcatc aattacattt ttgatcaaac gtaaaattga tgggttatat
    32221 ggttggtaca agtaagaaac gcgttcgttc atacggtctg ctgccattgt gtattgaatc
    32281 aaatcgttcg tacctacaga gaagaaatct acttcttttg caaacttgtc tgctaagact
    32341 gctgctgctg gaatctcgat catgatgccg acttggatcg tatcagatac ttccttgcct
    32401 tcacttatca atttttgttt ttcttcttca aagatcgctt ttgctgcgcg gaattctttg
    32461 agtgtagcta ccatagggaa catgatacgt aaattaccat gaacagatgc acgaagcaat
    32521 gcacgcattt gtgtacggaa catttcgtcg ccttgttcag ataaactgat acgcaatgca
    32581 cgatatccta agaatggatt catttcatga ggtaattgca gatatggtag ttctttatct
    32641 ccaccgatgt ccattgtacg aacaactaca ggttttcctt ccataccttc taatacagct
    32701 ttgtaagctt catattggtc atcttctgtt gggaagtctg gagaatccat atacaggaat
    32761 tcagtacggt acagaccaac tgcttctcca ccattattat ggacaccaac tagatctttt
    32821 ggtgttccga tgtttgcagc taattcaaaa tgcttgccgt cagctgtgac agtttctgca
    32881 ttttttaatt tttcccattc tgctttttgt gctgcgtaat ctgcacctgc tttttcaaat
    32941 tcagcttttt gttcatctgt aggatcgatg attacatcgc cttcgatccc gttgacagcc
    33001 aaaatatcgc cttctttgac tttagcagta atttcttttg tcccaacgat tgctggaatt
    33061 tctaaagaac gtgccatgat agcagaatgt gaagtacgtc caccaatgtc tgttacaaac
    33121 gcttttacaa aattgcgatc taattgtgct gtatcacttg gagtcaagtc atgtgcaaca
    33181 acgaccactt cttcgttgat cattgatgga tttggtaaag taacgcctaa taagtgcgct
    33241 aaaatacgtt tagcaacgtc acgaatatct gcagcacgct cttgcatata agcattatct
    33301 tccattgctt cgaacatacc gatatacata tccgttactt cttttagcgc tgattcagca
    33361 tttacgctgt tgtctttaat attttgcttg atttgaccta ccatttctgg atctgaaaga
    33421 accatcaaat gggcatcaaa tacttgcgct tctgcttcac ctaagctttg cgctgctttt
    33481 tcacgaattt gctgcaattc ttcagtagat ttagctaatg cagcatccaa acgagcttct
    33541 tcagccgcag tatcttctac tgttgcctta ctgaatgtta aatccggttg aactagcagg
    33601 taagcttttg caacggctac accgtcacta gcggcaatcc cttttagcat ttcaaccatt
    33661 attcagataa gccttctttc ttcattgttt caacgattgc tgccattgca tcagcttcgt
    33721 cagcaccttc tgcagtaata gtaacatcag aaccttgacc tacacctaaa gacataacgc
    33781 ccatgattga tttcaagttt actgatttac ctttatactc taagtttaca tcagagttaa
    33841 atttgcttgc tgtttgtact aatagtgtag ctggacgagc gtggatccca gtttctgcta
    33901 ctacgtgaaa ttctttcttt tccatattaa tcggtctcct ttaagtgagt gttgttttta
    33961 tgtgagggtt ttctttttta gtaattcaga gaatcctaac aaatgccacc ctttcatctg
    34021 taaagattaa catttttttt cgttagatac aacagttttc attcaatttc agataaaagt
    34081 attcaaatta ttccggattc tttttcatcc ttttcttcac attttataac aaacgtgttt
    34141 atttcctttt tcttgtcgct ttctcttctc ctgacgttta tcattctttc ctttgaccct
    34201 ctttgttttc atatgaaacg aattacttgc ttcccttttt atttttagta taataagagt
    34261 caaaggtcag gaatggtcaa tagagatttc accttttcta cctcataaat ctcttaaaaa
    34321 actaggggtt attcttttac ctcatgaaaa acgagatgta aaatatggat gtacccattt
    34381 ttgtgaaagg acgtgaatga ttcatgcttt gtcaaaactg cggcaagaat gaggcaacga
    34441 ttcacttgta tgccactgtc aatggtcagc ggacacaact cgattactgt cagagctgtt
    34501 atcaaaaaat caaaaatcaa caaaatggag gtctgactaa tatggcccaa aatgatccat
    34561 ttggatttgg cagtttggac gatcttttcc gttccatgtc tcgccaaatg caacagcaag
    34621 gaaattatga gcaaacacca ccaacacaat ttggcggaaa caacaacttc aatggtggtc
    34681 agccacctca aggcaataca gaaggtttat tgggagaata cggaatcaat atcacagaac
    34741 aagcacgaca aggtgatatc gatcctgtga tcggacgcga tgacgaaatc aaacgagtca
    34801 ttgaaatttt aaatcgccga accaagaaca atcctgtctt gattggagaa ccaggtgttg
    34861 gtaaaacagc agttgttgaa ggtttagcac aaaaaatcgt agatggtgat gtaccgcaaa
    34921 aattaatgga caaagaagtc attcgtttag atgtcgtatc tttagtccaa ggtactggta
    34981 tacgaggaca atttgaagaa cgtatgcaaa aattgattga agagatccgt caagcagaaa
    35041 atgtgatcct attcatagat gaagttcacg aaatcgtcgg tgctggttct gctggtgatg
    35101 gaaatatgga tgctggtaat attttaaaac cagctttagc tcgaggagaa ttgcaaatgg
    35161 ttggtgctac taccttaaat gagtaccgca tcatcgaaaa agacgctgct ttagaacgtc
    35221 gtatgcaacc tgtacgggta gatgaaccaa ctgtcgaaga aacgatcagt attttgaaag
    35281 gtttgcaaaa acgttatgaa gactatcatc atgtaaaata tactgatgag gccatcgaag
    35341 cagccgcaac tttatccaat cgttacgtcc aagatcgctt cttgccggat aaagccatcg
    35401 atttgctaga tgaaacaggt tctaagaaaa acttgacgat ccaaatcgtt gatcctaaaa
    35461 caatcgagaa aaaactgcaa gaagcagaag aacaaaaagt tttagcttct cgagaagaag
    35521 atttcgaaaa agcagcttat tatcgcgatc agatcaataa acttcaaaaa atgaaagaac
    35581 gtcagctcac tgaagaggaa acacctgtga ttaccgaaaa agatatggaa aaaatcgttg
    35641 aacaacggac aggtattcca gtgggtgaat taaaagaaaa agaacagaca caactgaaaa
    35701 acttagcaga tgacttgaaa gctcatgtca tcggacaaga taacgctgta gatcgagtag
    35761 cgaaagcgat tcgccgaaac cgtgttggat taaacaaaca gaatcgtcca atcggctcct
    35821 tcttatttgt tggaccaact ggtgtaggta aaacagagct tgcgaaacaa cttgcctacg
    35881 aactattcgg ttcacaagat tcaatgattc gtttcgatat gtctgaatat atggaaaaac
    35941 atagtgtctc taaattgatt ggttctcctc caggttatgt cggatacgaa gaagctggtc
    36001 aattaacaga aaaagtacgg agaaatcctt acagtcttgt cttgttagat gaagtagaaa
    36061 aagctcatcc agatgttttg catatgttct tgcaaatctt agatgatggt cgattgacag
    36121 atgcacaagg aagaacggtc agctttaaag atacgatcat cattatgaca agtaatgccg
    36181 gaactggtaa agtagaagca aacgttggtt ttggtgcggc acgcgaaggt gttactcgtt
    36241 ctgtattgaa ccaattaaac aactacttta caccagaatt tctgaaccgt tttgacggga
    36301 tcatcgaatt cagtgcatta tcaaaagaaa acttgatgac aatcgtcaca ctaatgctag
    36361 acgatgtcaa ccaaatgctg gcagcacagc aacttcacat cgaagttcct acgaatgtca
    36421 aagaaaaatt agttgactta ggctatgatc catctatggg tgctcgtcct cttcgacgca
    36481 cgatccaaga acaaatcgaa gatggtatcg ctgaatttta tcttgatcac ccttctatcc
    36541 atgagttgaa agcaaaactg gataaagatg gaaaaatcat cgttacttct aaaccagaac
    36601 gtttggctaa agaatcagcc gaagaaacgg ctgaataaat ccagttttat agcgtgagac
    36661 ggtatttctt tgagaaatgc cgtctcattc ttttttcaga aaagtcaaaa tactgttcat
    36721 tttataaaaa agaaattata ataagagaaa caagcaatta aaaatacata aaagaggtga
    36781 gcgccatgaa attggtaaat gtaacaaaca gccattcccg tcttgtatta aaccagcttg
    36841 aaaacactga tgcccatcta gtcaaggtct acacagccgg aaataccact attgtctaca
    36901 ctgaagcccc tgaacataat gaaattctgc tgatcaatga caaacgtaaa atccagccga
    36961 aagaaatcga agatgctaaa aattatttct tagataaaat caaagacgcc gtttaccacg
    37021 aggatgaaat caaagtaatc gaattggcag gccttgttga aatctcgatt ccaaaacatg
    37081 aattaggcgc tacattagcc gtgtaaacaa attttgaaca taataattaa agagcgtggg
    37141 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc gaaagcagaa
    37201 gcaactcctt cggaaataag ctgaaattca cgaaaattgc ttttcaaatt tttgtgaatt
    37261 ccagcttatt tcttggagtt aaacgcttct gtctcagcct ctttttcttt ctagcttttt
    37321 gatttttctc ttttcttttg tacttttctc ttttcatttt cagttctacg cgtgtcttta
    37381 tttttaactt tttcctcttt atggatgatt gatcgtactt tttgtgcaat ttgctcgttt
    37441 ttgtcccagt cttttacatc taataatcga cataatcgta acatctcttc ttttttctgt
    37501 ttttttcttt cacgtctttg ccattctgtt tccatgtgtc acactctcct ttctttaatc
    37561 atatgtacgc aaaataattt ttatattttt ttgattagac atttctatga ttttaatata
    37621 gtaaaaagag ccgtcacgga aaatc
//
LOCUS       DACDRN010000019        35134 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDRN010000019 DACDRN010000000
VERSION     DACDRN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.019"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(56..1432)
                     /locus_tag="IWJ80_001214"
     CDS             complement(56..1432)
                     /locus_tag="IWJ80_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9460958.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(1469..2335)
                     /locus_tag="IWJ80_001215"
     CDS             complement(1469..2335)
                     /locus_tag="IWJ80_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP9460959.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(2424..3449)
                     /locus_tag="IWJ80_001216"
     CDS             complement(2424..3449)
                     /locus_tag="IWJ80_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9460960.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(3467..4141)
                     /locus_tag="IWJ80_001217"
     CDS             complement(3467..4141)
                     /locus_tag="IWJ80_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9460961.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(4325..5218)
                     /locus_tag="IWJ80_001218"
     CDS             complement(4325..5218)
                     /locus_tag="IWJ80_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9460962.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(5292..6989)
                     /locus_tag="IWJ80_001219"
     CDS             complement(5292..6989)
                     /locus_tag="IWJ80_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9460963.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(6982..8295)
                     /locus_tag="IWJ80_001220"
     CDS             complement(6982..8295)
                     /locus_tag="IWJ80_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9460964.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(8295..11813)
                     /locus_tag="IWJ80_001221"
     CDS             complement(8295..11813)
                     /locus_tag="IWJ80_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9460965.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(12106..12564)
                     /locus_tag="IWJ80_001222"
     CDS             complement(12106..12564)
                     /locus_tag="IWJ80_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9460966.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(12861..13379)
                     /locus_tag="IWJ80_001223"
     CDS             complement(12861..13379)
                     /locus_tag="IWJ80_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9460967.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(13381..13997)
                     /locus_tag="IWJ80_001224"
                     /pseudo
     CDS             complement(13381..13997)
                     /locus_tag="IWJ80_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(13994..14848)
                     /locus_tag="IWJ80_001225"
     CDS             complement(13994..14848)
                     /locus_tag="IWJ80_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9460968.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(14845..15756)
                     /locus_tag="IWJ80_001226"
     CDS             complement(14845..15756)
                     /locus_tag="IWJ80_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9460969.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(15797..17131)
                     /locus_tag="IWJ80_001227"
     CDS             complement(15797..17131)
                     /locus_tag="IWJ80_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9460970.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            17339..18169
                     /locus_tag="IWJ80_001228"
     CDS             17339..18169
                     /locus_tag="IWJ80_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP9460971.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(18413..18662)
                     /locus_tag="IWJ80_001229"
                     /pseudo
     CDS             complement(18413..18662)
                     /locus_tag="IWJ80_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18853..19047)
                     /locus_tag="IWJ80_001230"
     CDS             complement(18853..19047)
                     /locus_tag="IWJ80_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460972.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(19552..19737)
                     /locus_tag="IWJ80_001231"
     CDS             complement(19552..19737)
                     /locus_tag="IWJ80_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460973.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(20587..20928)
                     /locus_tag="IWJ80_001232"
     CDS             complement(20587..20928)
                     /locus_tag="IWJ80_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9460974.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(20928..21158)
                     /locus_tag="IWJ80_001233"
     CDS             complement(20928..21158)
                     /locus_tag="IWJ80_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9460975.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            21445..21783
                     /locus_tag="IWJ80_001234"
     CDS             21445..21783
                     /locus_tag="IWJ80_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460976.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(21988..22227)
                     /locus_tag="IWJ80_001235"
     CDS             complement(21988..22227)
                     /locus_tag="IWJ80_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP9460977.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(22203..22550)
                     /locus_tag="IWJ80_001236"
     CDS             complement(22203..22550)
                     /locus_tag="IWJ80_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP9460978.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(23282..23863)
                     /locus_tag="IWJ80_001237"
     CDS             complement(23282..23863)
                     /locus_tag="IWJ80_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9460979.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(24046..24672)
                     /locus_tag="IWJ80_001238"
     CDS             complement(24046..24672)
                     /locus_tag="IWJ80_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9460980.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(24694..26025)
                     /locus_tag="IWJ80_001239"
     CDS             complement(24694..26025)
                     /locus_tag="IWJ80_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP9460981.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(26180..26581)
                     /locus_tag="IWJ80_001240"
     CDS             complement(26180..26581)
                     /locus_tag="IWJ80_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9460982.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(26667..26873)
                     /locus_tag="IWJ80_001241"
     CDS             complement(26667..26873)
                     /locus_tag="IWJ80_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460983.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(26968..28470)
                     /locus_tag="IWJ80_001242"
     CDS             complement(26968..28470)
                     /locus_tag="IWJ80_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP9460984.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(28488..28943)
                     /gene="argR"
                     /locus_tag="IWJ80_001243"
     CDS             complement(28488..28943)
                     /gene="argR"
                     /locus_tag="IWJ80_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9460985.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(29041..29658)
                     /locus_tag="IWJ80_001244"
     CDS             complement(29041..29658)
                     /locus_tag="IWJ80_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9460986.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            29879..31252
                     /locus_tag="IWJ80_001245"
     CDS             29879..31252
                     /locus_tag="IWJ80_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9460987.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            31327..32439
                     /locus_tag="IWJ80_001246"
     CDS             31327..32439
                     /locus_tag="IWJ80_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP9460988.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(32511..34148)
                     /locus_tag="IWJ80_001247"
     CDS             complement(32511..34148)
                     /locus_tag="IWJ80_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP9460989.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(34316..34924)
                     /locus_tag="IWJ80_001248"
     CDS             complement(34316..34924)
                     /locus_tag="IWJ80_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9460990.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT        10033 a         7470 c         6039 g        11592 t
ORIGIN      
        1 ttacaactgt tgatgggcgt ttttcttatc tttcttctaa cttctccatt ggagtctatt
       61 tctcattcat cttgctgtgt ccatagccat agccaaaata aatgatcagt ccaataacca
      121 ttacgatacc gaacacgatc catgtcacag tttgcagacg aagcatcaat acaatacaag
      181 tcaagatgga gacgattggc aatagcggaa cgaatggcac tttgaattct ccagcttttg
      241 gtttgccaaa catttttctc aacttcagta agcctagact aacaagcatc aaataggcga
      301 gcgccatgat attcgttagt tccgctaata cattcaatgg gaaaaatccg gcaaagaata
      361 cagctgccac accagcggca taagtcgctt ttttcggtgt acgatgcttt tgatcgattt
      421 ctgtcataaa cttaggaagt aagccatcct tgctgacagc aaacagtaaa cgagcaagtg
      481 aatacatcat cgagatcgtt accgtcagta gcgttaaaat tgcccctaca gcgatcaccg
      541 cgcccactgt tccatgtcct acatacctca ttgcaaaagc cactgggtca ttgactccta
      601 gttcagtata cggaacgatt cctgtcaaga tcagcgtcac gatgatgtaa aggacagtag
      661 caatcagtat cgaaccaatg attcctcttg gcacatcttt ttgcgggttt ttgacttcct
      721 cggctgccat tgcaacggca tcaaatccca agaaagcaaa aaacacaaga gctgcaccat
      781 ctagcacacc ggaaaaacca aaaggcatga atggctgcca gttatcaggt ttaacgaaaa
      841 atattcctac aatgataaac aacgcgatga taccaaattt gacatatacc atggcattat
      901 tcaaacgtaa tgctttcttg gcttcaagcg acacgatgta agtgacgaat atcactacga
      961 gcaccgcaat caagtcaatg tatgtaccat tttccggatt gtacccgcca gttaatgctt
     1021 gtggaagttc ggcccctaaa cttttcaaaa aaccttgcat atagccagac catccggaag
     1081 caacagagga aaccgctagt aaaaattccc cgatcaacaa ccaccctgtc agccaggcag
     1141 caaattcacc aaatacaaca tacagatagg catatggccc accaatcact ggcactcgcg
     1201 aagcaaattc tgcgaaactt agtccagaca aaatgacaac gattgcagct aagacaaatg
     1261 aaagtgacag tgctggacca gcattttgat tggccgcaac ccctgtcacc acaaatattc
     1321 ccgtaccgat gatcgcacca attccaagca tgatcaaatc agacgttttt agttcttttt
     1381 tcatctcact cgtatgattg agattgatct ctttcttacg aaacaaatcc attgaagttc
     1441 ctccccattt taggttggaa aacagcgatt aaactgtttt catataccct aaaaggatac
     1501 cacctaaaat gactaaaagg caaccaacaa taacataaat gagctctttt ttcgtttttc
     1561 tttcgccaag gatgaaaatc ccacctaaag tagagataat gatccccatt tgtgaaagtg
     1621 agaagccaac agctaaacca agtgatttga ctgttagaag catgcagatg tttcctaagc
     1681 cccataataa tccacagatc atgtttttcc acacaaactg atcgacttta actttacgga
     1741 aagcaaagaa gcttgcgcct aagatcattc cgatactttg aggtaagata attgacatcg
     1801 catccaaatt cgcccagttg acgatgatgg tatatacacc ataaccaatc gtagagaaga
     1861 tcaatgctct aaaaccctta ccaaaatcaa gcattttatt atctgtatct atttttcctt
     1921 cgtcatcttg tcgggcagtc aaatacgcac cgcttacaag aagagctaaa gcaataatac
     1981 cgtatacata atctttcgtt tgtgtccatt catggaagaa aacggctccc gcaatcgtat
     2041 tcaaaattaa ttgaaaacca gtagacaatg gtagccctac agaaacaccc atgtacttca
     2101 ttccatggaa ctgtccattt tgtccaacac tccaagctaa gccggataaa aatccgatca
     2161 agaaaatcca aggggtgatt acaggactaa caacgaaaaa gacaactaaa gaaaacagaa
     2221 atgcaccgat cgtcattcct agtgtttgtt gattggcatc gccaccaatt ttaccactta
     2281 ctaaaccaat actaccccat gccaacatag ggactaatgc tattaaaatt tccataacca
     2341 atctcctttt atttcacatg ataagaaact tatcagcgaa aacgttaaca acacaagcac
     2401 tttttttcgt ttctaacgat tttctaacgt ttttcattat aaaaatttaa gattgggaaa
     2461 aataaagtca ttgttgtccc ttttccttca gttgattcag ccgtcaaatg gatacctaat
     2521 ttttcggcca gattttttgc taaataaagt cctaatcctg ttgaatgtgt tttgctaagt
     2581 cgaccatttc tcccagtaaa tcccttatca aagatccgtt tcaagtcttc cgctgggata
     2641 ccgatccctg aatctttaac tgaaaggctg actccttgcg cttccttttc tatgctcaca
     2701 atgatgtttc cgtgatccgg cgtatatttg atggcattac taagcaattg gttgaagata
     2761 aaagcgatcc atttagcatc ggtcaaaaca gtatgatcct caccaacgat ctcgtagcga
     2821 atattttttt ggataaaata atttgcctga ctgcgtatca caggctgaat gattcctttt
     2881 agtccatatt cttggatgag gtagtcccgc gagaaactgt cgagtcttgc ataatacaaa
     2941 acttgttcta cgtattcatc gatttttgat aattcatttt ctagtaatgt atacttttga
     3001 tcgtcaatat cgaactcgat cgaacgaact agcaatttag aagcggaaag tggcactttg
     3061 atttcatgga cccatgagtc aatataatct ttttggtctt gttgattact gattgctgat
     3121 tgcattactt cttggtgttc acggatcagc tgattgatat attcttctac cagtttttct
     3181 tcttctgtcc tcccaccact caaataattt tgcaaagggg aagttttttg tatgtcggct
     3241 aattttcccc accatcttct cttgctcaag taaccaccca gcagataaaa aatcagaaag
     3301 acgccctcaa tcaaagctaa ataagcaatt gtgctcaaat caactttgat gtttggcgcc
     3361 agccacatca caaagatcgt caatgcaata aaaaacagcc atcccaagat caatgtccac
     3421 tgatctttca aatatttaaa aaaattcatc ttaaccgctc atcctttcag ggaacgatat
     3481 atccctgtcc tacttttgtt tcgatatatc cttggatacc tgcctgttcg atttttcggc
     3541 gtaaccgatt gatgttgact gtcaatgtat tgtcatctac aaatcgttcg tcatcccaca
     3601 gtgctcgcag caatttttcc cgacttaaga tctttccatt gtgcttcatc aaaataaata
     3661 atagacgata ttcatttttg ctcaaatcaa tgatctctcc gttgatttcc atacttccgt
     3721 tgtcaacatt caacgtaatg ccattgtggc tcatcatttc actagataat tctgtgtagt
     3781 tgtaagaacg gcgaagtaat gcattgattt ttgcaatcag tacatcgatt tggaaatgtt
     3841 ttgtcacaaa atcatctgcc cccatgttca tagccatgat catatccata ttcgtattcc
     3901 tgctggagat aaaaatgatc ggtactttcg atacttcacg tattttttga caccaataat
     3961 agccatccaa aacgggaaga ttaatatcta gtaatacaag ctgtggttct tctctttcaa
     4021 agtctgagaa cacttgattg aaatcggtcg ttccaaaggt ttcaaattgc catttctcaa
     4081 gttcctccat gatcaattgt cgaatgatct cttcgtcttc gactaccatg atttttgcca
     4141 taaaaaatcc ctccatcttt cttcttgcta ctacaatatc aaagaagccc ttgtgaaaca
     4201 agttggctta cctggaaaat gtcattttag caaacgttaa aaaaaactgg aaatatgcga
     4261 tttaatcgcg tttgtctctt ccaagcagct taaatttatt atgttattct taataattgg
     4321 tctattatct gttatttttc tttcgttgtt tttgtctttt aaattttcga taacaccaat
     4381 aaccgaaaag cgttagcact agcaatatac ctagaaggag tataagtaat cgataaagat
     4441 ggtatttttt tgtctgttct atctttgaag agacttcttc tgtaaaggga actcgtcttc
     4501 ctgtcactag taatcggtga gtgttcacgg tgtaaggtgt acaagtgata agtgtgacta
     4561 gatcctgctt ttctctaatt gctaatgtat ttgtttttgt tggaaggaca acaattttct
     4621 caacgacttc ataagcgagc atccgaccat acacttcaag atagaatcgg tcatcttgct
     4681 ccatttcttc aagatccgta aagagttttt tctcagcaat accgctatgg gcggtaatca
     4741 cggaatgtgt actctctcct ccagttggaa aagaggtgcc ttgcaggact gtcgctcctc
     4801 tatctaacaa aagatcattc gtttcatcaa ataaaggcaa gctaacattg attttcggaa
     4861 tatagatggc tccgatcata tgttgttcgt aatattcttt tcctggattt tggacatttc
     4921 taaccgcttg ctcaaaagga tctttgactt gtcccattcc tgggattact gatgtatgag
     4981 caagcgtctg atttttcttt tcatgcactg ctaattgttg ctcatgtttt tcttctttat
     5041 cgcgttcaag ctgcttttga taattctcga tccttctttg atccaaataa ttgctgagag
     5101 catctgctaa aaatggatat gtaaaaactg cagctccggc aagaaacatc actgccataa
     5161 ataattgtaa taaaagactg atttgggttt gaggtctatt acctattttc tttttcattt
     5221 tcttcacctt cttatttaaa aaaactgcag caacctttat aaaaccgatt gctgcagctt
     5281 atttttgttg cttatacttc agacttcatt ttagatttac ggtaccagct gtacgctcca
     5341 atcatcaaac ttattccgat tagtaagaat gccaagagcc cgttcccacc agtagcagga
     5401 agtgacccgc gtttcatatt cggtactctt tctggccctg gattttctac tcctacgatc
     5461 tgaatttctt cattatagct tccataagag atgataaatt cagtaaaggc tgcctcacct
     5521 gttggaagta cataaccttc tggcgccatg gtctctttca tttgataggt tccataatct
     5581 aacccttgaa cctcaaagta tccatttcca tctgcgacaa attctgttgc ttgtccgctt
     5641 gttgcccact caattctatc tgctcctgat tcataaacag ctaaaggcga ctccccttta
     5701 taaaatattg cgtattcaga acggtcttca ttccacaatt cgaatcttgc atctttaagc
     5761 aattcactgc ttgatgcatc atatttttca aactttcttc cacctgttgt gactttctct
     5821 ggcggctcta ctccttcttc ataatcaaat tcatcatttc gtccgatttc aataaccgct
     5881 gtgttgttat ggaaatcatc cgcttctagt tctgtggaaa ccttcaaatt atacgtgatc
     5941 gttaaatctc ctccacgtgc atctgctaat ctacctaaag tatctacttt ggctactcca
     6001 ttttcttctg tcaaaatcgt cacgacaaac ccgttgctta ctacttctac tatataatct
     6061 tgtccttttg ttaaaatcgt ctcgccggct cgcacttcta tttcctgatc ataataagtt
     6121 agaccaactg taggcgtatc tgtgattttg taatttttga taacagcgct tccgttttca
     6181 aggacagaat cgatgaaata aggaataggt gctgtgatcg tgtaagaagc cgtttttcct
     6241 ctttctagat caatataaga aatcgtttct tgttctccag cttcattaat aatcgttact
     6301 tgtctaaggt cgctttcctc tacattcatc tgtttatctg cttctctgat ttcgtttttc
     6361 ggatacaaat gcacatctgt attgtatgtg tctttccatg tagaaccatc ccacacgcca
     6421 tccaccatat caggcatcat cactggcatc ataacaacca ttggagacgc gattctggaa
     6481 atatgagctg gcgaataagt ttctaagaaa agatagactt tatctctaga agaaggtgct
     6541 tcttccgtca cttggacggt agagaatacc gcttctccag cttcattcgt tgtttgtttg
     6601 tcaatacttg tcagtcgata ttttaaaaac caactttctg tgtgccgttt ttgaatccat
     6661 tcaatcgctt cggcagaatt aagaccgtct tctcgttccg gtgttaaagg attatctcga
     6721 atcaaatcgt aatatacttc cgttacgtca aacaaatcaa aatcgactcc tggaatattt
     6781 tcttcaccaa aatccgtaga aaccaatccg ctattcggtc gttcttccgg aacagatgag
     6841 aaaccttttt tgtgtaatgt aatcgtcact tcacttggtt tttgtgcgac ttcttcggct
     6901 ttgactagtt ttccgttccc gaaaatccct attcccgcag taacgagtaa cagtacactc
     6961 attcccgtga caatcatttt tttattcatt tttttctccc cgttcttttt ttattaataa
     7021 aacagcacct aacaataaaa gaattcctag taaagaaatc aacgttcttg cttcattcgt
     7081 ttgcgggaat acggaccatt ttcctgattg acctggattg cttggaccac tcggctcttt
     7141 cggtttgtcc gttgtttctt ttacggtctc gttataaaca cgtggtgttg cggattcata
     7201 ggcagagact ggaactttcc cggattctaa ttcagccatc tcctgtccgt taactactac
     7261 atattgtggt tggccttttg catccaccca aaattcaggg atgatgacct cgattctctt
     7321 acttgcttca tccatctcgt aaccttccac accttttaat tcttcaaaat aataggtgcc
     7381 agaaggcaag aaacgttgtc ctgtcgtaac aaggccctct ttatccgaaa tgaatttgct
     7441 tacatttttg tcattcaaag gatcattgtc tgctggtttg atccattgat ttttcaagtc
     7501 attagcagga gccatatcta aataatattt ccccccttct atcatctgat atagagcaaa
     7561 aacagcacct tctaatggtt ttcctttttc agttgttcct ctttgacgtc catacttgaa
     7621 gaagtatgga tctcttagat aaccgacatt tttaggatag atatgaatct cttgcagttc
     7681 ctcggttgat tctgtcgggt gataaattgg caaaatagca gcaattggga tagctgtctg
     7741 atcgatatca acatccaatc caacttctgg atcaatcgat tcttcgaaaa tcaaataaac
     7801 tgcatcccga tcttggtttt tcttaggtgc tttgatacgt gcaatcccgt ctcctaatcc
     7861 gccaaccgta tctttcccag ttgttaccga agtccctatt tggacaagcc tttcttctgt
     7921 gattagctga cggatattgt ttctgttcgt ttcagcgatt tgctggacga attctttagg
     7981 agtcatgttt tcagcagctt ttttttcatg ataataatcg gtcatgtcgt aaatggcaaa
     8041 tgtcgctcca tttagtggca ccgtctgtga aatgatatct gctgtttccc ctgcttcctc
     8101 agagatagcc aagccatcat tttcatggta ttcaaattga tcgttataat cgatatctcg
     8161 aatcatccgt ttatgcaaca cgaattccac gacatcttcc cctgattctt ctgccgacat
     8221 cgcatgtgga agaaggactg ctgcaagcgg tagtaaaaca agtaattgaa gcatcaaaca
     8281 caacagcttt ttcattacct tgctccctcc tttttagatt gtctgacaaa caaaccatag
     8341 gctgctcctg caagcgccat gaagaatgca gcaagcagga tatagaataa tcgtccggat
     8401 cctcctgtag caggcaacgg attttcagga tcgttcccga cactaagctg taatctgatt
     8461 cgatttgtct ccaaaagttc atattctaac tcttgctgta gatcaggcgt ctctgcttct
     8521 tcgtttggat aatacgaaat ctcaactttt agtgtacccg cttcttttcc tggaactacc
     8581 cgatcatttt ctatgtcagg ttcttctcga taactaattt ctaaaataaa gtaacctgga
     8641 agttttctat agctgtcagg tgacttcact tccttgatcc gatagacccc ttccaacaag
     8701 tctgaagcat tgaataggga agaatcgttt ggatctggtg ccaagcctcc ctcgatgatt
     8761 tcatactctc ctgcactatt ttgtttctca attgaaaatt ctgcgccttc aagacgtcgg
     8821 ctattcatac tgtcaatttt caaaagttcc aaatcgattg gcgctagttc atcttctaag
     8881 gttacttgta gtacaccatc gatgatttca tagccttccg gcaagggagc agtttcgtct
     8941 tcgatgaccg tatcttcaat gataaactta ccttcttcat tcagtgcaaa ctggaacact
     9001 tgatcagata gccgataccc atttggtgcg acagattccg agacctcata ttcaccaata
     9061 gacagatgca gaggttggtc gtaatttaaa tcgacatact tttctactcc tacggcacga
     9121 gaatagaagt atccgatccc aggcaaacgt ttcgattctt ctggttctaa cggattacca
     9181 gtgtatgctc gatacagcgg gaaggaagaa tcccccactt tcctgatatc gaaacctgca
     9241 ccatctaatg tatggccttc atggtctaat tttctaactc ggatctcccg ataatcaaac
     9301 gtattgtaag cagtgaatgc tacccataaa cgatcctctt catctgttgt atacgtgaga
     9361 tattgccaat tttcttcacc tgtttgcttg agttggatag tccctatagc attatcgtaa
     9421 agtcgcaaga tccatgtgtc attttcatca ggttctttga aaccttctgg gttctggctc
     9481 tcaatgattt tgtattctgc atagttggta gtttcttcac tatccttata aacaaatgaa
     9541 tctggctctc cgattggatg aataactttc tcaccatcca cgattacata tttcccgtct
     9601 tcatctactg aatagaacgt gagtctccct ttctcttcct ctacttcgtt cacaccagaa
     9661 agccctttcg ctactggata tccgatttcc agtccgttac catctgtttc ataaagttca
     9721 aactctgcgc cattgattgg catattactg ccttcaatta gtttattgat attcatgtgg
     9781 attggcttga attcatttcg catttccagt ttgacataca actcatcata ctctgtgcct
     9841 tctatgttag gctcttgtac aacagaaagc ttgtgatatt ctccttcata ataggaattc
     9901 ggatctttac cttccggtcc gccgactctc gaaccagatg tatattcatc tacaagctcg
     9961 tagtgaccta acatctcatc ttcgttggct gcatattttt tgatttggaa ttgatacgtc
    10021 tcatctacta actgataacg tggtggtcca gcgatctctc tgatccaaaa tatgtcgctg
    10081 actgctccag tttcatctcc taaaatcggc atcttcgttt caaagatcgc tattccgtcc
    10141 gtggtagttt tacttgtttc gattggttcg gcttccccac tctctggatt ttggtcatat
    10201 aatgcaaatt cgactccagc caacggctcg ccattttgac ccttcttgct gacttggaaa
    10261 ttacggcctt tatgttcgta acgaatggaa gaagatggga aatacatccg tccagtagtg
    10321 atatcattct cataatcctc caggttctga atataggcca tattgttcac tgtgcaaaaa
    10381 tttccgtctt gagctcccgc atcaagtttt acttggtact tgatcctgac catttcccct
    10441 tcacctaaag aaatcggaga aaatttataa gcttggtttt cccgtccgac atacgttgtc
    10501 ggagtcgcca ctacttctgg tggggcttgt ccatgacgtt cttcatccca tatttctggc
    10561 tcttggtact tcatgccgtt ttctcctgga tctagatggt aaaactgaat atctgccgat
    10621 ctgccagctc caaatggtga gacattaaaa ccagtttcat cataaagtgt ggcttgctgg
    10681 atcgttctct tgaaggtaga agagatatcc tccaaggctg taacaatgtc attcttgttg
    10741 ttcgcattgt aatatcgcgg tgttacgcca tcttcttcta aatcagcaat atttttcaat
    10801 acatttcgtc caatagcatc tagtcgttgt ctcccgctaa cgtttccatc gattcctaaa
    10861 ccaatggaga agatacttgg cgggttctct tgatacttgt ttttcagcca atgatgataa
    10921 gcaactgtgt tgatcgcaga tgacttttct tgcgaagtag gactgccatt cgtattgtta
    10981 ttagaagaac catcaccaac ccagtgagtc gctttattat ctccttcttt tacctctcca
    11041 taacgatagt gtacatttac ttcatcttct ggacggttaa agtcagatgc ataggtaact
    11101 ggatgagtaa accctgcatt agaagtattc ccagaaaact cctcaaaatt tctgaacaaa
    11161 acgtcattat cttcagcaat cttatcttcc atcacagtcc agttgctcca agctccatta
    11221 gtgctcgtac gataacttga ctgaatcggt gcataactga aggtcggtgt cccatctccc
    11281 acaacgatta ggattttttc tggatttcta ttttctccgc cattgtcttt atacaatgtt
    11341 tcatagccat ttttaagccc tagtgtaagc ggagttcctc cgattggtgt acgatttaat
    11401 gctgtattct cacttcccgt caaacgattg atatcatttg tcaatggaaa gatgtccgaa
    11461 ctgatttgct catggttatt gatagctgta ctattatggt aaaaattcac cataccgatt
    11521 cgaatatcca gactcgggtc agagagcaga tctttactca tttcgtttac tgcctcaatc
    11581 aacgcagcat ttttagtttg actattggtc ccagcggtta attcactcat actcgctgac
    11641 ttatctagaa caaaaacgat atcgattgga ctgatttcct ttttctcgcc cccgatgaca
    11701 tctagataga cattgtaaag ggttgttgca tcttcaggat gtagatcaga tgctgtgctg
    11761 cttggcagaa caggttctac ccattttttg atgtacgccc cgttataatc caagtaactg
    11821 ttttcaaaat tccgttcatt tccccaaagc tgaacggttt gagctggaac gtttctatct
    11881 tcttcgcctg cagttgatcc attattagta gcatagtcgt aatttcgcca gttcgctccc
    11941 ccgtttgtat aatcactatc atcgatcaag ttctctccgt catatggcga gcctttgtct
    12001 ccttgatagc gttcattgct ttgtgtccaa tttcctaaca cttttgtata gtcattcgtt
    12061 gtaaacttca tctattcttt catcaaccac cacttgaaga ggtagttacc cttcgttcac
    12121 atcggggatc ttttctgttt caaaaatcaa tgtgccggtt tctgcttctt ttgaataaac
    12181 ttttccgaca taccattctt gaccatcgat cgtctcgact tttaggtctt ctccatcttc
    12241 atcataggag gatagacctg ttccacgaac gttttttacg gtaccaatcc cggcggctgc
    12301 cgcatcgatt gctaaacgta ttctaccttc attggcactt tccttttttt caaaagccag
    12361 atgccattca agggtttctt ctttttcaat atacttccct tcaatttttg catagtcctc
    12421 atctaagaat acctcatcct cctttgtttc tgttgactca gcagctacat ccagggtcgt
    12481 catatgatag actgactgga aatatggtcc aaatagcaaa ctgacagtta ctaactgcaa
    12541 aagcttcaat tttaaccctt tcacatcctt atactccttt caaaaatatt tgtcttcttt
    12601 cacaacaatc aacaaaacaa tgcccatcta attataattt aatttaattt taattttaaa
    12661 tcaaatgata tcctttttag ttaaaaacac caattataca acatttatta ttaaaattaa
    12721 attataaaat tttataacgt tctcatttaa caaaacttaa gttattaaga caaatgaata
    12781 aacaactacg ctttcctcga aacaaaaaaa taaagcacaa atgcgcattt acgcactcat
    12841 gctttattcg tacaattaat tcagataacg gcagcgcata ggatgccaat aacacttcct
    12901 aaaatagctc ctcctaaaat gtcagtagga taatgtaaaa gtaaaaaaat cctcgaataa
    12961 gcgatcaaac aagcaaataa aaaggctaat attcctgcaa ctggacttaa ttcgaacaac
    13021 gcagctactg ataaaaaggc tagcgctgta tgtcctgaag gaaaagaaga agtcttcggt
    13081 ttacttgtgt aaaggacaag ccgacgattt ttttcataag gtcttttccg tcttgtcgtt
    13141 tttttgataa aaagcaaacc aacaaaaaaa tagatcaaac tgccgataag aatggctaga
    13201 ccatattctc tagtatcagg tatcaagact aaaacagcag caaggagcaa ccaaataaaa
    13261 ccaccattag agatattact gataaatttc aaaaaattga tttttcgact atccgttttc
    13321 cgttgcttgg cgaacttgtt gataatacta tcatcgattt tagcaatcaa acgcaacatg
    13381 ttattcctcc tttttaagaa ccttttcttg cgtacgatgg ataaaaagca ggacttgaaa
    13441 ataaaacaag aaactgaaac ctaataaagc aaaatagatt ggtcctgcaa accagaatgc
    13501 gacaagaagg atcaacgtgc ctgccaaata gaaaagcgcg cttttgaaat cattaaaaaa
    13561 caagaaaaat gcagtaatcc aagaagcttc tttttgttcc agttgaagca agattttgta
    13621 actaaagaga agcgcgctta atagaagcaa aaacaacgta gtatacatca atgtatagcc
    13681 aattccccca ccccaatagc ggaaaatctg ataatcgatc aatagaagaa gaaaataaac
    13741 acttgctaaa ctatcttgct ttccataaag agagaagttc tcacgaaact ttttccagaa
    13801 tagtgaaccg agctttttct cttcattttt ttgtaatgat aaatcatata cagaactgtt
    13861 cgacttgcag ttcctaatgt gaaaacaggc aagacgcaca ggaaccatag tagctgtaaa
    13921 tggataagac gtggaatcca ttcaatgatt tctaagaaat aatcattggc ttttttacca
    13981 atcgatcgat tcatcatcct tttgaacctc ctgtcaaatc catcccttca atgaaataac
    14041 gttgtgcaaa aaagaacagc aaaatcgtcg ggatcaagac aagtgttgct cctgccatca
    14101 gataattcca ttgtgtcgtt gcttggttct gaaaaacttg caagccaatc tgtaacgtgt
    14161 atttttcttg atcttggata tataacagag gacctaagaa atcattccaa gctgcgttga
    14221 aggaattgat cgcaatggta atcaaggcag gtttcgtcaa tggaagcatc aaatgactcc
    14281 aaatatacaa atgattcgct ccgtcgattt cagcagcttc gatcaattca ttattgatcg
    14341 ataagtaaaa ttgacgcatc agaaatatat tgaacgcatt gccgaagaac gatggaacga
    14401 tcaggggcaa ataagtacct acccagccga tcttggagaa taatacatat tgagggatca
    14461 tcgttacaaa ccccgggatc atcatcgtag aaagaaccaa agcaaacatc agctttttcc
    14521 caggaaaatc aagcttcgca aatccataag caataaaaga atttgaaagg acattaccga
    14581 tcactactag cacagtgatg aataatgtat ttcttgcata gcgccaaaaa ggaaaggcgg
    14641 caatcgtatc cagatagttt tcccatacga ttttttctgg gaaaaaggtc ggaggataac
    14701 gaacgatttc ttccattggt ttcaatgaag taaacaccat ccaaagcaat ggaatgatca
    14761 aaatgattcc tattactgtc atgaggacat aacttagtat tttttttgtt tgccgattca
    14821 cactggtctt tttgaaataa tccatcattt gccgtcacct ccagaataat agacccactt
    14881 aggtgcaagc ttcaaattga caaacgtcag aaccaacacg atcagaaaca ggatcattga
    14941 catagccatc gcataaccca tatcaaaagc tacgaacgcc ttgttccaca tatgcagatt
    15001 ataaaaaagc aaagaatttg caggtccgcc tgttccattt ttcgtcatga cataagcttc
    15061 ttgaaaaatt tggaaagaac cgatcgtaga ggtgatcaca tcgtaaaaaa taataggagt
    15121 gattaatggc aaagtaatac ttttgaactt tccccaaacg cctgctccat cgatttctgc
    15181 tgattcatat aaagatctag gaacattttg taatgtagct aagtaaagta gcattgctcc
    15241 tccagaactc catattttca tcaaaatcaa cgcaggtttc gtccattgtg gatcaaacag
    15301 ccaagctggt ccagggatat ggaaccagcc cagaaatgtg ttgatcaatc ctgcagatgg
    15361 agaaagcagc tgcatccata agatgtagac tgctactcct gataagactg ccggtaaata
    15421 gaaagctgta cggaatacac tgattccttt gattttttga ttgagtactg tggctaaaaa
    15481 cacactgaaa atcgtcgtca gcggtacatt gaagatgacg aaataaacgg tgttgtataa
    15541 cgatttccaa aataaatcat cgtgaacgaa cattcttttg aaatttgcta gaccaatgaa
    15601 attcatcttg gatgtcaaat tataatcggt gaaactacca ataactgaaa aaagcatagg
    15661 ccctagcata aataccaaga acccgataat aaaaggcgag ataaataaat aggcccatct
    15721 tgcttctttt ttctttcgga tagataaacg cttcaaattt tcttgcctcc tctgtaccta
    15781 gacagtcaaa aatggattat tgattttgct tgactaagtt ttctactgct ttttgtgcat
    15841 ctgccaatcc ttcttttggt gattttgttc ctaaaagaat ctggtcaatc tgttcattca
    15901 ctaatgtcgt gtaatccgga gcatctaaag gaactggtgt gatcaccgta tttttaaagt
    15961 tttcgtccgc catttgatag atcatttttc ctttatccgt taggttgtca ttattcacta
    16021 aattctcatt ggctgtcttg ctcgccatga tatcaaaact tttttcgccg aatttttctt
    16081 gtacttctgg agaagaaaga tatttgatga attcataaga ggcctcagga tcttttgctc
    16141 cgtatggtac ttccaagacg aatccgccac cccacgacca atggcctgaa ccttcttctt
    16201 tttctgggag ctgcatcacg ccaaaatcaa aatcatccgg tgcattttcc atcaaactag
    16261 agtaataatt gatattttgg gcacgcatgg ctactaatcc ggaaataaat ggatcagata
    16321 caccagaccc aaattcggct tctaatttat tgatcgtatt tttcccataa taatcttgcc
    16381 agtcttggat ccactccaag gcttccactt tatttttagt atcaatcttg actttttcat
    16441 ctttgtcaaa ccaactagtc ccgttatctg cgtttagcgc ccatacatca gcccctaaat
    16501 tccataatgg ataaaaaccg atgcgtgtaa attcttttcc ttctttttta tctagtttac
    16561 gtgctgtttc ttctaattca tcccaagttg tcggtagatc ttgttcactg attccagctt
    16621 ctttaaataa ctttttatta taaaaaatga cttgtgtatc tgtattgaat gggatcgcat
    16681 aaggatcacc ttcatattct actgtatccc aaagctgcgg ataaaaatcc ttagaagtgt
    16741 ctttttctac atatttggag agattagttg cttgctgtgc ttctgctctt tgggcaacgg
    16801 aatttatgtc ctgtacaata acatcgggtg catttccagc tgttattgca gataaagatt
    16861 tagtccagat gtcaccccat ggctgatact tataatttac atggatctcg ttttgtgatt
    16921 gattgaaatc ctcaatgatt tcttcgatag cttcttttcg tgcaccggat ccccaaaagg
    16981 accagaaatc aatatttttt cttccatcac ttgttttttc agaagaattt gctgcacttg
    17041 aactggcatc gcttgcatta cttgatccgc atcctcctaa taaaaacaat gctgaacttg
    17101 ccagcaaccc tgtaaacaat aattttttca tattttacct ccaaatttta atgtaaccga
    17161 tgccggtttt aatataacga tatcattttg ttctgaaaat aacaatttat aaggtatgat
    17221 tctaaaaaaa ttattaatga taccaatact ttatataatt cgcgagcgct ttctttgaat
    17281 tttttaatct ttcgtagcag aatgagaatg tattaaaaat agattaggag gaattttgat
    17341 gtttagacac cagaaagaat tgcagtttga agcaaaacca gatcgtccga atccattaat
    17401 cgcgaaatat ctgcaggagc tgatcggcgg ccaatatggc gagatgagtg tagctatgca
    17461 gtacctgttc caaggctgga gctgccgagg agaagaaaaa tataaagata tgctgatgga
    17521 cattgcaaca gaagaaatcg gccacgtgga aatgttggcg acaatggttg ctcgtttatt
    17581 agagggtgcg cctgttgaag atcaagaagc agcgatgcaa aaagatccaa caatcggtgc
    17641 cattatctct ggaatgaatc cacagcacgc gatcgtagct ggcttaggac ctcgtcctgc
    17701 tgacagcgtg ggcaatccat ggagcggtgc ctatatcatt gcgagtggca acttgttagc
    17761 agactttaga gctaatttaa atgccgaatc acaaggaaga cttcaagtag cacgtattta
    17821 cgaaatgatc gatgatcgtg gcgtaaaaga tatgctgtct gttctccttg caagagatag
    17881 ctatcatcaa aatctatggg aatgtgcagt gaaagaatta gaagaaaaag aaggttcaat
    17941 cttagtccca agtactttcc cgcgtgacaa agaacgaaaa gacattgctt atgacttcta
    18001 taacttctca gagggagacc aaagtagtga aggttcttgg gctagtggca aagcactgga
    18061 tggcgaaggc gaatatcaat ggttgaaaga acctaagatc gaaggcgctg caccaaaatt
    18121 agaaccagta acaccaaaaa tgtatgggac acctccattc aagaaataaa tatctacccg
    18181 tagaaacacc cgttttttgt tccacacgct ttgtgagcaa aactgactaa agtcattaat
    18241 cataaccacc cttcaaaagg gtggttttct ctagagctat aagctcaaat caccggccag
    18301 cgcctaaaga cgctggcttt catattttat gttcaagcca tattgccttt gccagttttg
    18361 cccacctctt aaaaggttta tgtactactc cttagccctt aaaaggatcg tcttactctc
    18421 gtacactcag cttatctaac gcactatcat gtttttcttg ttcttgaatg tactttttga
    18481 tggtcgcttc attttgtcct acttttgtca catagtatcc ttccacccaa aagtgtcgat
    18541 acccaaattt atattttaga tttgcacaat ctcttgatga tctcctgaat attggctcga
    18601 tattgattat aaattatttt tcttctatac tttggtgtga atacaatgtg gtatttacac
    18661 atctactttg tacgggctaa actatttccc tttattgctc cacccgcata gcaagtggtt
    18721 ttgtatttca atcgctcggc gcgctcaatt aggtctaaag acaataaatc taaaaacccg
    18781 ctgcttcact gacagaaaca acgggttttc agaaaaaaat gaaatgaaaa agatctctta
    18841 cggcaatcta ttttattttt ttgatttttt ataaatggaa aatgcttcaa gaagtccaag
    18901 aaataaagcc gtataaaaac aagaacctat gaaatcttta tttattagat attcaacaga
    18961 aatcccgata acactagcca acaatatatt tatcagaagt gtaaaataag gatgcttttt
    19021 atagaataga actagtgttg ttttcataga ctctcctttc ttcatatttc caattaatca
    19081 caaattcacg gcattactag aaaaattaaa ggggcgcaga agtggaaaaa tactcttcga
    19141 ctttccctac aaaccaggcc atcttgtgtt taataactat accaaatcta aggtacctct
    19201 aaatctaaat tccatttgtg gcggtgacct ccaattttta actcttctat ctcccatcca
    19261 gtatacataa ccttgatact tcgaccactt atatatcgaa cacacaaata cctatccatt
    19321 gcaggagtac tacctaactt agttgctact tgcatctaaa taacagctga aaagatagga
    19381 cttactctta gtaaagtatc tgaactatta atttttaaac tatcattttt atcctcaact
    19441 acatgcacat ctgttgcaac ttcactttct gtctcatcca gataaatgat taaggaatcg
    19501 ggaattgcaa tatcttcagc tgacgctgga atagttgagc taaattagtc atcatatctt
    19561 tattataaaa taaccaacag caaaaaatac aatgaaaaac aaacttggaa ctaatgtatc
    19621 attaataaat gttgaaaaat tcagcaactc attactgtat gattttagca gcccaaccaa
    19681 catataccct ataaaagcta ataagatggt taggaatcct ttagaatttt tactcattca
    19741 tagacacttc cttaaataac ttttattata gaaggtattg taaataataa tattgacaaa
    19801 aagtgactca aaataaaaaa aaacttacat tatactggaa tgaactgtat tgatagtgta
    19861 cctaaaagct ctactgacat aagatcgtgt aatcggtaaa gtaattttat taattatacg
    19921 ggatgtaatc aattagagaa taaactaatc ataaaaggta cttttacatt cataattgtc
    19981 gctttgagat tctactttgg ttcactatct gacccaattg cacattttcg atcattacaa
    20041 gtattggtgt cgttagaaca actaaataaa aactagcggt agatttttca gaatctatgc
    20101 taatttctgt atggatgttc cagtgatggc aaatttctct tttcattgtt tttcttgtcg
    20161 catgatatgc ctttttgtgt tattcagtta aactatagaa attctttcct agacagatct
    20221 tgatcccaaa ttttacgttt ttgagcatcg atatagttta aaatgacatt actcatatct
    20281 aaagagttta gattttttag gactaaaact gtcagttgtt ctttttcttc tttctgttaa
    20341 agtattgtcg cgacttttcg tatacgttaa gatatgtcgt gaaaaaacaa gttttgtgac
    20401 acaaaaaaac gaatgacttg aggaaatcac ctcaatcatt cgtttaaaat gctatatatt
    20461 ctttagttta agatacggaa attaatgcca tcgaccacac tagtagaatg aacgacattt
    20521 acttggcctt tttttaattt cgttatgttt agcatataaa aatattatag agtttactta
    20581 caatgcttaa aacatatact gtataatctg atcaatttta gccatgtctt gcaatggtaa
    20641 gttttctatt tttttgagtt ttcgttcctt aaaatcaaga gattttaatt gtgtaataat
    20701 tacttgtcct tttatatcta gctcttcagg taatgagtaa cgtgtcggta aattctttat
    20761 agtggaagta attggacata tgaccgaaaa cattgtttta cggttaaatt cataacgaga
    20821 gacgactagt cctggtcgtc ttttttgaat ttcttttcct acggagggat caaaatcaat
    20881 ccatacaata tcaccttttt taggaatata aattctttct tctgacacta taaaatttct
    20941 ctcccttcag gagttaaatc ttcccattcg tctttctcat aatactcggc ctcttcccca
    21001 ccactaaatg gatcttcaat ttttggaact aacgtaattg ttccatcatc agaatacaca
    21061 acgatatatt cttggttttc tgaaggtttc tttccgttat cagaaggtaa agttaccact
    21121 acagaacttc cttgtaatct agattttgta acaagcatta tcatcactcc cttaataaaa
    21181 gtatactata taacactaag taacgcaaaa ttcccctttg taaactatct agaaaattgt
    21241 tttgtttaaa gtatatctta ttggatataa atagaaataa attgtagcca gttcattttt
    21301 tctttaacta aaaaaataat ccaagaaaca ttgaacctat ggatccaaaa gctacccaaa
    21361 tactatagct aaccccccgc taccttcaat tttgcagata aaacgctttg ggagaaaatt
    21421 tcaaagacaa caggccatcc tatcatgccc gattataaac aaaaagctgg ctattccttt
    21481 tgctctgaca aatttcaatt gggaaagttc acgaaactag caggtgaaga agtttcaact
    21541 gttcggatca aagaatgccc gcttcaaata gaaacattgg ttcaacgaat ttcaactaga
    21601 caagactttg ctattgtaga acgtgagatc gtagctattt ttgtagaaga agggatttta
    21661 catgatccaa ctcatatcaa tgtggataaa tggcaacctc ttatctataa attcagagaa
    21721 tatacaactg ccaaccagtc gttaactttg aattttagat tccaagaatt caaagagtca
    21781 taatgataaa catcgttagg tataaataaa aaagactcgc agcacgagta aaaaacaaat
    21841 agaagctata cattagaaag gtcgactgtg accctataga tgcagtatat gcagcttatg
    21901 agcatttctt ctgttctctt tgtttttggg atctgtgctg aagtcttttt cgttttttgc
    21961 cgctctttct ttgaaaaatc gattatgtta taccttctcc attactaatt caggcatctg
    22021 ctgagtaatg atttttcgcg caaaagaaat atctgttacc caaggttcga tttgttcttc
    22081 agtcaagaca agcggcattc ggtcatggat tggtgcgact gtgcggttag gttcagtagt
    22141 cattatgatt gattctgcca ttttttcatc ctgatggcta cgataaaaac cgccaatata
    22201 cactattttt tccttatttg aaaacataaa tttgtctttt tcttcattcc actcattggg
    22261 aaaacacaac gattttgcaa aaatggttga tgaaaggtct ttttttcttc tactgtctct
    22321 gcacgggcat taatcatcaa ttgtcctttt ttaaatccgg taaatcccca cttcatgatt
    22381 tccggagtga ggatgttttc ttcctctttt cttaacgtca caacaagatt tgacggaaaa
    22441 acttctccag ctgctaattc tgctgcctgc ttcggttgtt tcgtctgggc ttctcgataa
    22501 taattctgaa ggagctctga ctctagatca aaaaaataac gaccacacat cctaatccct
    22561 cccaatcttt ttttctatca tacactgcaa caagaagttc gcaccaacaa aacgctttca
    22621 atttttgtag aactaaaaaa gttgtgacaa gtgacaaact ctccagtgaa tggtattctc
    22681 ctgccaactc ctcggtcaca agtcacaata ttcaacaaac atgagaagct gctttttgaa
    22741 attgttcctt gtgactcgga gtgaaacggc aggcttacga cctctgggta aacggtgttc
    22801 aaaagccagt ttctcggtat taagccaaat ttatgcaaaa atgtagaaag ctattttcac
    22861 aaaatttttc ttaatactcg aaactaagcg actttttcac aacctcttcc ttaaacggtg
    22921 ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg aaaaatatga gcagctattt
    22981 ttatttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc tccactaaac
    23041 gatgttcaat cagctaacct tcggtattag ctcaaaaaac ggcaaaatat gaggaactat
    23101 tttgccgttt gtttctctaa tactcaggtc aaagcgctga tttcacaacc tctaatacac
    23161 ggtgttccag aagtagcttc ttcgaaaaat aagccgaaat ttcacaaaaa tttgagaagc
    23221 aattttcgta aattccttct tatttcttga agctgaccac ttctgtcaca acctctaatt
    23281 attaaagaac cttttgcgaa gttaacaatc tttctttgat caattgaaaa ttctgcctaa
    23341 gtagtgatag atcctgtgtg actttcaaca ataagatact tccctggatt tcagcaatca
    23401 acgctttggc ttgacggtct gcttcttcat cggacagttt ttcttcagaa agtgccagga
    23461 tattttgttt cgtatacttg acccattgat tgactaattc tagtagcaac agacggtatt
    23521 cttcatccac tgtactcaac tcaataatca aattacctac aggacagcca tgataagtga
    23581 tctgtgtctc atgatcagcg aaaatccagt ccaacatttc ttctaatgct tcttctggct
    23641 gttttccagc atttaagatg tcttcgaaca attctgtctg ccatacgtcc atatgcgcat
    23701 ggatcaccgc tagacaaact tctttttttg aagaaaagta ataataaaat tgaccttttc
    23761 ctgtatcaga agctgctaga atatctcgga ttgacgtttc atgatagcct tttttataaa
    23821 taagatcttt ggtcgcttcc aagatctttt cctttgtttt caatgatatt ctcctttttc
    23881 atttctcata gtcctatatt atacagtaaa ttcatcattt tttcttctta atcgtttaaa
    23941 aaagactgtg ccaaattatc tgacacagcc ctcactataa aactgttctc atatagctag
    24001 atgttcattg attcaagaac tgcgaggaca aaagcaattc ctatcttatg atagttgctc
    24061 taatacttcc tctgttgtca tcacttgatc tgcaataaag ccaaagttcg tcaacgctgc
    24121 atccattgct tcttggccag gagcaccaat tgcatctgtc gctaccacaa cattgaatcc
    24181 actctctacc aattcacgca tatgggaatc gctacacaag tttccgttca ttccaccaag
    24241 aattactgtg tcgattccat tttttctcaa ttggagcgcc aaatcattgc tttctggtcc
    24301 aaagatttta tgaggagagc tgatgatcgt attttcatcc aaataaggtt tcaattcttc
    24361 gataaaatcg gctcctgaac cttcttccac tgattcatat tgactttttc tagcaaacat
    24421 ctgctgctca agcatcatat gttctcctgc accgccaaat tgccatttat ggtcatgtgg
    24481 gtaaaaataa tgaggagaaa taaagagttt gtatcctttt tcttttgctt gttttaccat
    24541 ttcaatcata ttttcgatcg tattgtattt ttctaagata tcttttgtca aaccaaagcc
    24601 accgcctgct ggtttcaaga actcgacttg cgggtcagtg agaactactg ctgtattttt
    24661 attattgaac atggtggatt ctccattcgt tatttatttt aatacatcat ttagagcttc
    24721 actcattgtc ggatgagaat agatttgatc tctcaataac gtataaggaa gttttccatt
    24781 catagctagc gaaatcaagt tgatgacttc atgagaatct tccgcataga tacttgcccc
    24841 tagaatcaag tcagtttcag ggtcgactaa aactttgaat acacctttag gatcttctaa
    24901 tacttgtgct ttagggacac cgcttgtcat atacttgaat aatttgtaat tgacgccttg
    24961 tgctttggct tgtttttctg ttaaacctac attggataaa gtcggagtga taaatacact
    25021 gtaaggcaca gctttccggt tattcgttgt tcgtttattc tcgcccttca actgatccat
    25081 aatgattcga taatcatcta gagagatata agtgaactgc aatccgcctt tgacgtctcc
    25141 aatcgcccaa atgttgtctg catctgtacg aaggtattca tccacagcga ttgctccgcg
    25201 atctgttagt ttaacatctg tattttctaa tccaagtgat gctgtacttg gttttcttcc
    25261 agttgcagcc aaaacttttg acgcttggat tgttacttct tggccatctt ctgtaaaggt
    25321 gacggcagct gttgattctt ggtctgctac ttgatctaca gatacaccta gatggaattc
    25381 gattcccgca tttgtcatgt cttctaaaat catttgcgcg atatcttcat cttcacgtgg
    25441 aagaaattca gaatgggcat caagaacgac aactttagat ccgtattcat tgaacattga
    25501 tgcaaattcc aagccaatat atccagcccc gatgatcact aatttttccg gtaattgttc
    25561 ctgatccatc gcttgcgtag aatcaatcaa gtagcgactt gtattcaatc ctttgattgg
    25621 taaaatgatt ggttcagaac cagtattgat gaaaatccgt tcgccttcaa caatcacttt
    25681 ttcaccatta ttcgtcactt cgatttgatg gttagaaaga aaacgtgctg taccgtccat
    25741 cactgtccca gttgcctcat cgctgatcat atgataattt ttggcgcgaa gcatgcccgt
    25801 caatttttct ttccgagcaa cagcctctgt aaagtcgatt cctctttctc cattgaaaat
    25861 caatgattta gaaggaatac agccgatgtt gatacatgtt ccgccataca tacgtgttga
    25921 cttttcaacg actaaaaccg attcaccttt tgaagctaac gccttcgcta aagtcttacc
    25981 gccctttcca aaaccgatca caatattttg atatttttcc atcatttttc cctcgcttct
    26041 tttttaccta ccgattggta caaaaatatg ataaccgata attgtctatt ctccaaatca
    26101 tctgctttat ttgtcaaaaa aaaccagaca aaaagcggtc ctttcacgtt tttagtctgg
    26161 ttcgatttcc tttattcttc taaacaatcg ttacttctac atcgatattt cctctagttg
    26221 ctttggagta agggcagaat tcatgcgcat cttctgcaat tttttgggct tcttcttcgc
    26281 tgatgccatc gatatgcact tctaatacta caccgatctg atagccgcca tcatctaaat
    26341 tataaagaga gacacgtgct tttactgtgg actctgctgt cattcctgct tctttgatca
    26401 tatgttgtac cgccccattg aaacaagaag cataacctgc tgcaaataat tgttctggat
    26461 ttgttccttc tgcatggatt cctggaggag tgattttcat atgcatcgag ccatttggtg
    26521 cctcgacttc tccattgcgc ccgccatggt tgatcattgc tgtttcgtat gcttttttca
    26581 ttgtactgct cctttcgatt gaccatttgc tacttggcta taattatagt gtatgacaag
    26641 taaggcgctt tctacaaaaa ttctgcttat tcgaatacca gaatgccgtc ttctactatt
    26701 tttccaaaaa acaaaaaaat actactcgtg aacaacagcg tcatatttgt tccaaaataa
    26761 atagcaatct tttctacata atcttttata tgattgattt taagatacat atataccaga
    26821 aagttgaccc cataaaccaa tactgttaaa aaaataacca tattcgttac cattttttcc
    26881 tccttttgct ggtctcccta ccagcactcg atttaaccat accacttttt ttgccgtcca
    26941 agcaactagc ctttcaaaaa gactggttta ttctgctgtt aatgattcaa gatcagaaaa
    27001 atgtccgaat aattttgcta attttactaa cgccaacttg ttctttccga attcatcatt
    27061 ttctatctgt aatactagta acggctcatg taagcttaaa cggatcaaaa accagccgtt
    27121 tccgtatggc tcttctagat catatcgaat accttcttca ttttccgggt cgaagttcat
    27181 ccctttttct tcttccaaca gtcgcttcat ttcttctaat atcttgtttc cttgttcgcg
    27241 tggttcatcg ctgaacaatt ttaatcggat ttcttgtact tctacgggct gtttcaactc
    27301 tttgatttgt tcatctaatg tgataccttt tctttttaat tctggcaaaa gcatcaggat
    27361 ctttgcgatg acatatgcac catcatccag aaaatagttt tccttgaatg cagcgtgtcc
    27421 gcttgtttcg atggctagct gtgtatcttt cccaagacgg ttaagttcta atgctttatt
    27481 gattacattt cgatagccgg aaatataacg aacttggatg cctccttttt cttcaatgaa
    27541 tctttgtaga tgagaagaag taggggaatt cgtcacgatt cttgttcctg gatgctcttt
    27601 taatacgatt gtacttaata ctgcgatcaa attatttcga ttgatcactg ttccagaagc
    27661 actgacaact gcagaacgat ccacatctgt atcgaaaatg atcccaagat ctgcttcatt
    27721 tgctaaaaca gcctgcttga tactttccat cgcttcttta ttatcaggat ttgggatatg
    27781 attaggaaat cggccatctg gttccaaaaa ttgggaacct gttgaatccg cacctaagct
    27841 attcaatact ttttcagcaa agaatccacc agcaccattt ccggcatcaa caatgatttt
    27901 ccaaccattt agtggtttgc tttctagtgt tccaatcccc tttttaattt ttgtgactaa
    27961 atcttgcgca taaggtgtca aaaggtctgc tttttctacg cttccagttt gttcgctttt
    28021 tattttttgc tgctgtgata ggataaaagc aatatcttct ttttctgctc ctccttctga
    28081 ggtaaagaat tttattccat tataataata gggaagatgg ctagcagtca gcataaccgc
    28141 agcatcgcac gagaactgtt caaattgagt ggacataaac atagcaggcg tagtagctaa
    28201 ttcgaaatcg atgacatgta tcccttgttc tttcagactt tggatcaacg cctctttcag
    28261 tgctggtccg cttatcctac tgtcatgacc aatcccaatc gtccattttt tatttggatc
    28321 ggttgatttc ttctttttct ttatccagtt tgctagtccc cagccgatct tttttaccat
    28381 gtctgtcgtc aagtttactt catatccttc aactggtaat gcgattcctc gaatatccga
    28441 tccattttgc agcgctttca tatctttcat ataaaagtcc tcctcattta ttccctcatt
    28501 ttttccagct tttctgcaaa tcgctgtgct tcttcttctg agcggcaaat aataatgaat
    28561 gtatctacac cggccatcgt tccgacaact tcagggtaag ccacttcatc tagccaattc
    28621 gttaccacat cggcaccatt tcgttcagtc aatacgataa ccatgaactg aacagactgg
    28681 atacgcaaaa cctcgcgtct aattgctgta cgcaaacgtt cttcatttaa agaaacagat
    28741 ggctggttaa ataatgtata gcgtactatt ttgttttcat tttgactttt tactaacttc
    28801 atttctcgaa tgtctcgaga aatcgttgct tgagtcacat cggcgcctgc ttcatgcagc
    28861 cgcgcaagca gctcttcctg tgtcccaata ggttgttctg taatgatctg gcggatcagt
    28921 gcttgtcgct gtttcttttt catattagac acctctcaat gttgattata gcaaaagata
    28981 agtaatattt cttcatattt tggtattttt tacttttatt ttttgttttc attcaggttg
    29041 ctaacctttt tctattttaa cacgcaaaat cgtcttcatt ttttcaggtg ctgattttct
    29101 tggatcgctg atgtaaatct ctttgtgttc tttagaagct ctttcatagc cttcttttgc
    29161 gatgaatgct tctaatttct caaacgttgc tggttcttcg gcatagggcc cgacgtgtaa
    29221 gatttgggca caaagacctt cttccatctt tttgaatact acttgttctt tcaagtcttc
    29281 cggaagtttg ctagaacgct gaatggcttc ttgtgctatc gcttctgtaa caaagtcagg
    29341 ctgtcggatc atcaattgat aagaaaaatg ttcttttttc atcactggtt catgcaaata
    29401 cttttcttgc agtccccatt gtccttccag tggatagaca gtatacatct gataatcagg
    29461 aatcagccaa ttttttttag gcgacatgcg tattgcatag ctgactgcat atagacaggc
    29521 tactcttcgt ttaaagtctt cctgattcgg gtcgccgatc cctctgatgc taaagaatga
    29581 ttttttagga acctctagta tttgcgggct ttttttcata ctatagctct tgtctttacg
    29641 ccattcaatt ttttccattt ttcttcctcc gcgaaattcc atttagttat ttattatcat
    29701 acctcttttt gagtgacttg gctaatcgtg ttgacactat ttttcagttt gtgctagaat
    29761 aagtgaagta aataaatcgt acagataaca cgtttcggcg tgtcgagaga gaggcggttt
    29821 ctatttttat aatagaaatc actctctttt tttacgaaat tttttagagg tgatgtaaat
    29881 gacaaaacaa aaacagctgc ttttagtacc tgcagtaatt gcaacaggga tgatgtcttt
    29941 tgcgggcgta ttgatcgaga cggccatgaa tgtaactttc ccaactctta tcagacagtt
    30001 tgatttaacg actgcacaag tccaatgggt aacaacgatt tatcttttga tgatttcgat
    30061 cattgttccg ctgtctactt atttgaatcg caattttagt ctcaaaaaac tctttttagc
    30121 ttcaaatctg cttttcttgg caggtataat gatcgatttc ttctctccta ccttcatggt
    30181 cctattgttt ggtcgattgc tccaaggtgc agcgacaggt atcgctcttc cgttgatgtt
    30241 ccatattatc ttgacatttg cccccctcaa tcgacgaggc gcgatgatgg gtgtcggtac
    30301 tttgacaacg gccattgctc cagcaattgg ccccacttat ggaggcatta tgacttcaca
    30361 ttttacatgg aatcatatct tcctgtttct tgtgcccgtt ttgattcttt ctttgattat
    30421 cggctggttt gctattcccg agatggcagt acaaaaaagt ggttatttgg attggataag
    30481 cgttttagga atttgtcttt tgtttagtgg aagtctaaca tttttgagta cgttgggaac
    30541 agtttccagc tggattgctt taatcatcgg cggtattggc tggttccttt tttactatcg
    30601 aagtaaaaaa gtggagcatc cactgatccg actgtctatt ctttcaaata aaacattccg
    30661 cttgtttttg attggcttct tagtttatca gtttttatta cttggtgtat cttttgtctt
    30721 gccgaatttc cttcagatcg ttcaaggaag tagtcctttt gtcgctggat tagcgatgct
    30781 tcccggcgca gcggttggtg ctttgctttc tcctttttcc gggaaaatgc tggatcaata
    30841 tggtccgaag aagccaattt ttgctgggct gattctttct ttgattggtt ggctagcatt
    30901 attcctactc ataggacacg cttcagtggg tgtattgatt gcttgtcatg tgttttatat
    30961 gatcggtatc ggattatctt acagcaatat gatgaccacc ggaatgaatg cactgaatga
    31021 agaactacaa ggagacggga acgcaatgtt caataccttg cagcagtttt ctggcgctgt
    31081 ggctacttca ttagtagcgg tcattatcaa ttacgtccag catcatactt cccataacta
    31141 tgaagtgtct actacattag gatcaaaagc agcattaggc gtcttgttgc tgttgcttct
    31201 cgtaagcttt gctcgattcg cctattatct gttctttgct aaaaaagcgt agattccata
    31261 tacggcaggt gttgcatctg ccgttttttt gtgtgaaaat ggttgtttgt aaaaggaggc
    31321 ttttttatgg aaaaaattgc cattatcggt ggcgggatca ttggtatgac cctagccagt
    31381 tacttagata catcaaaatt caatgttgct ttatttgatg aagggacagg acaggcaact
    31441 aaagctagcg ctgggattat ctccccttgg ttgtcgaaac gcagaaacaa acaatggtat
    31501 cgtctggcta aagacggcgc tgcttttttc cctaaactta taaaagattt ccaacttgat
    31561 caaacgattt acgcacaatc agggacactt ttgcttcgac ctgaaaaaga tttagctgaa
    31621 ttggctgttc ttgccgagaa acgaaaagaa gaagcgccag aaattggaaa catcgaatgg
    31681 ctttcacctg aacagacatc tcaagcactt ccgttattga aaccagcagt ttcactgcgt
    31741 atcagtggtg gtggtcgtct ggatggaaaa gcatatttag aacatcttgg tcagcgttta
    31801 gcacaaaaac aaatggagca gatccaggca aaagtcactt tgcataagaa gagcgattct
    31861 tggctcatca agggaccgtt tggagaatac acagctgatc gtgtaatttt gacacctggc
    31921 ccggcactta agcctttatt acaagaatta ggctatgctg cagatatacg tccgcaaaaa
    31981 gggcagctct tagcatttca gacatcttat aagatgagtc aagactggcc tgttgctatg
    32041 cttgacggag aagctgattt cattcctttc atggatggaa agatcctttt aggtgctacc
    32101 catgaaaacg atcaaggctg ggatttggct cctactagcg aagcttataa gggattgact
    32161 aagagtacag aacagttctt gactgatcca gatcagataa aaaactggcc atacgagttc
    32221 cgtgtaggta cccgtgctta tacgtcagat ttttccccat ttttcggcgt actgccagat
    32281 gatccaagct tactggctgc tagcggtcta ggctcttctg gcttgaccac aggtccttat
    32341 atcggctatc tattagccaa cttcttaaat actggtcatt ggcctacgga agattatcaa
    32401 aaggacatct ccacctatat tacgaaaaat ccatcataga aaaacagctt tttcaagtaa
    32461 ggatcccgag cgatattgcg catggattac ttcttgaaaa agctgttatt ttagttcaat
    32521 ttctgaatca aattctccaa actttctttt gcttcatctg gcaagagatc ttttcctatc
    32581 tgagcttcaa tgaatcgtaa acgaatatca agtcgttgtt tcaccagatt tttataggac
    32641 gtatcctcaa aagaaacagg aaaatctggt ataggtaaat agtggatgtc ttcaaatgga
    32701 ccaaacgact tgaattctgc cgtttgttgc acgattcttt caacgattga caatgtcgtt
    32761 tcaggtttga tttttttatc atcgaaatat ccagtattca ggacatagca ggctgtttgc
    32821 tgtttcaaaa acaactctcg aaaagcttga tagtcttccc taagaggata gacacggaaa
    32881 ggattagcaa atggttcaat gactagctca tctagctgtc tttttgtagt gatattttcc
    32941 gcgtctgatc gttttgttgc taatgttgca ccaaaagacg ctgctaaaac tgggtcattg
    33001 atcttgataa ttggcggaag cgaatcatct ttcatgatcc aaaaaacagc atcgatcttt
    33061 tcgctaagat gatcgactcg gtcgggtgtc gcataacgtg atttgactgt ccgcccattt
    33121 ccgtttcgta tatctcctgt tactaggact ttttctcctt tacttgtaca tgtcacgccc
    33181 acattctgac aagtaaggaa agaagaaatg gccggatcat cagacggata gtcttgtgtt
    33241 ttatcaaagt aagaaggttc caaggcagta gcaaaaccat tttttcgaga aataacgaaa
    33301 gcatcgtcat gcaagatcga aacagagtga ccttccgtat gttttgctaa ggtaatcgtt
    33361 gattttccag aaccggacaa cccaaataca gccatcgtat aggtctggtc attactagca
    33421 tattgtttca tcccaccgtg gcaggcaata aagccattgc gatttcccaa tgcccaagca
    33481 agcgtcaaag tcgctttttt taattctcca aagtaacgta atcctagcac agccgctgta
    33541 ttatggagag catcaaacaa aatgagtcca tttggaaatg tttcatgtgt ccattcggga
    33601 tcacaaaaga tataaagatc tccctcatca aaggaaacag acttttgata agtctctaag
    33661 atcttcgaat tcgctggctg aaaattcagc aaataagaat acaaattgtt ctcaaatcct
    33721 tctggaatca gcaaatgact ttgcacagaa aattcttcag ataggccaac gattactttt
    33781 gtatggtaaa actggcgctt gcgactttga tacacagctt ctcttaaaat tttctcgtat
    33841 ttgctttgtt ccttgtctaa gtcctcaaag atccgccgag cacttgctgt tcttcccaca
    33901 atgcttccat cattgaatac tagtgtccta acatcagctg gtaaatctag ttcttcagca
    33961 tgtgagattg ttaaatctgt gatgatcgtt cctggactcc cttttgctag ttgataagct
    34021 gtcttcctat caaaaacttc atgaacatta ttaccgtaaa atgtcgtttc tacaatcgat
    34081 cgaatactgg ataacagtgg atttccactt gtgatttcgt ttaattcgag actctctttt
    34141 gtactcatgg ctcctcctca ttccctaaac aaaaaaacgt tttctttatt atacattatt
    34201 cgagttttta tttgtactgc tgcgctgtgg gaagcaaacg agttattttt cacacaaaaa
    34261 aaccgagaac ttgaataaac aagtttctcg gtttcatcat tttcctttta tcggcttagt
    34321 accagccgtt tgctaaccag aatgttttag cagcatccca tgaaccataa cgagatgtta
    34381 catattgttc tgctacacgt tcttgattgg cagctgaata gtcgccgttt aagtatgaag
    34441 catctaattg ataacggccg atgtaacgtc cgttagtagc tgtgtatgaa ccgcctgatt
    34501 ctttttgtgc gatccattct tttgcagagc ttgaagttgg cgctgttgca gcagctgggg
    34561 cagcaggagc tacttcaact gtttcaacag gtgcaacgta tgtatgttct tgcgttgctg
    34621 tttcagttgt agatgctgta ttttctgttg ttttgttttc tgttgttgct gaagaatcat
    34681 ttgaagttgg gattgttaat tgttcaccaa cataaatgcg gttgatgttt gcgatcgaat
    34741 ttttttcagc aattgcatcg atcattgagt tgtctcctgc aaatttttgt gagatcttag
    34801 ataatgaatc acctgatttt actgtataaa cttcatcagc gtgagcattt gtagttccta
    34861 caaacattgc agtagcacca gcagcaaaag ttgtacctaa aacaattttc ttaaatgaat
    34921 tcatattaaa ctcctttttt tctccgtttt ttattctttc gacgctatta actttatcat
    34981 ttagaaatat ttcataggta aaagaaacat gacatttaat gacaaaataa cgaattattt
    35041 aatatttgta acatttaaac aaatattttt ttgatacaaa accataaaag ccttgttgta
    35101 taagcaaagt aatcgttttt ctatgttttt ttat
//
LOCUS       DACDRN010000020        34821 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDRN010000020 DACDRN010000000
VERSION     DACDRN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.020"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            423..581
                     /locus_tag="IWJ80_001249"
     CDS             423..581
                     /locus_tag="IWJ80_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9460991.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            653..1879
                     /locus_tag="IWJ80_001250"
     CDS             653..1879
                     /locus_tag="IWJ80_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9460992.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(1968..2360)
                     /gene="rpsI"
                     /locus_tag="IWJ80_001251"
     CDS             complement(1968..2360)
                     /gene="rpsI"
                     /locus_tag="IWJ80_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9460993.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2376..2819)
                     /gene="rplM"
                     /locus_tag="IWJ80_001252"
     CDS             complement(2376..2819)
                     /gene="rplM"
                     /locus_tag="IWJ80_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP9460994.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(3338..3805)
                     /locus_tag="IWJ80_001253"
     CDS             complement(3338..3805)
                     /locus_tag="IWJ80_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP9460995.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(3925..5463)
                     /locus_tag="IWJ80_001254"
     CDS             complement(3925..5463)
                     /locus_tag="IWJ80_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP9460996.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLSGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5543..6229
                     /locus_tag="IWJ80_001255"
     CDS             5543..6229
                     /locus_tag="IWJ80_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP9460997.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(6430..6957)
                     /gene="msrA"
                     /locus_tag="IWJ80_001256"
     CDS             complement(6430..6957)
                     /gene="msrA"
                     /locus_tag="IWJ80_001256"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9460998.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(7041..7718)
                     /gene="pgmB"
                     /locus_tag="IWJ80_001257"
     CDS             complement(7041..7718)
                     /gene="pgmB"
                     /locus_tag="IWJ80_001257"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9460999.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(7715..8845)
                     /locus_tag="IWJ80_001258"
     CDS             complement(7715..8845)
                     /locus_tag="IWJ80_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9461000.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(8845..11028)
                     /locus_tag="IWJ80_001259"
     CDS             complement(8845..11028)
                     /locus_tag="IWJ80_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9461001.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(11025..12050)
                     /locus_tag="IWJ80_001260"
     CDS             complement(11025..12050)
                     /locus_tag="IWJ80_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9461002.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(12095..12922)
                     /locus_tag="IWJ80_001261"
     CDS             complement(12095..12922)
                     /locus_tag="IWJ80_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9461003.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(12919..13986)
                     /locus_tag="IWJ80_001262"
     CDS             complement(12919..13986)
                     /locus_tag="IWJ80_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP9461004.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(14012..14848)
                     /locus_tag="IWJ80_001263"
     CDS             complement(14012..14848)
                     /locus_tag="IWJ80_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9461005.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(14845..15714)
                     /locus_tag="IWJ80_001264"
     CDS             complement(14845..15714)
                     /locus_tag="IWJ80_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9461006.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15784..17067)
                     /locus_tag="IWJ80_001265"
     CDS             complement(15784..17067)
                     /locus_tag="IWJ80_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9461007.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(17081..18766)
                     /locus_tag="IWJ80_001266"
     CDS             complement(17081..18766)
                     /locus_tag="IWJ80_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP9461008.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            19041..20066
                     /locus_tag="IWJ80_001267"
     CDS             19041..20066
                     /locus_tag="IWJ80_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9461009.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(20661..24314)
                     /gene="rpoC"
                     /locus_tag="IWJ80_001268"
     CDS             complement(20661..24314)
                     /gene="rpoC"
                     /locus_tag="IWJ80_001268"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP9461010.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(24393..28010)
                     /gene="rpoB"
                     /locus_tag="IWJ80_001269"
     CDS             complement(24393..28010)
                     /gene="rpoB"
                     /locus_tag="IWJ80_001269"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9461011.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            28738..29706
                     /locus_tag="IWJ80_001270"
     CDS             28738..29706
                     /locus_tag="IWJ80_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9461012.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            29724..30629
                     /locus_tag="IWJ80_001271"
     CDS             29724..30629
                     /locus_tag="IWJ80_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9461013.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            30626..31432
                     /locus_tag="IWJ80_001272"
     CDS             30626..31432
                     /locus_tag="IWJ80_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9461014.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            31589..32557
                     /locus_tag="IWJ80_001273"
     CDS             31589..32557
                     /locus_tag="IWJ80_001273"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP9461015.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(32874..33398)
                     /locus_tag="IWJ80_001274"
     CDS             complement(32874..33398)
                     /locus_tag="IWJ80_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP9461016.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            33685..>34821
                     /gene="scm"
                     /locus_tag="IWJ80_001275"
     CDS             33685..>34821
                     /gene="scm"
                     /locus_tag="IWJ80_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9461017.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDS"
BASE COUNT         9950 a         7383 c         6522 g        10966 t
ORIGIN      
        1 tgataattta tcttaacaac cagataattt tattcagcac cgtattatct atattgaatt
       61 ctcggaatca agataaattt ttttccgttt ttgtttcttt tttaatcttc ttcttgttat
      121 taatagtgat taggatgata ttcctatatt gatctttgat aattagatat aatttttgaa
      181 taacgttccc tgctattcga gctagaaata caagtacgcc atgacctagt tagcgacaac
      241 tacttagaga tttcttttgt tctgggacaa caaaatgcca tgacaatagc agtaaataat
      301 gatacttccg taaggctcgc cgtaaactgg ggagaggtga aattcctatg tgcttaccca
      361 aagcggttca aaaattttaa actattattt taggagatga ttttataaaa tcgaaagcct
      421 acttgaataa aaaacaagcc tgtgaatttc taggaattga taataatact ttagataagt
      481 ggattcgtgt atataatttt ccagtaataa aaattgatgg cgtagttcgc tttgacgtag
      541 aggatatcaa agatttcatg aatacattca aaaaaaattg aaattttctt ctcgtcacat
      601 atactataaa tagcgaaatg tactgcctta ctttttaaag gagtgaggaa gtttgcctac
      661 aaagctttca aatgggaaat ataaaactaa tctgaggtat ccaaaaaaat tcagagaaat
      721 cactggaatt gcatcagaga agtatcaaaa agtatttcca actaggcaat tagctattaa
      781 ggccgaaagg gaaatgaaaa agaagattga aacagtttta agagaggaga atgcaaactc
      841 actagaattg aaaggaaaaa taaaatttaa agatttttat gaatcaaaat ggcttcctag
      901 atatgaacta ggtcaaacta ctaggtcaaa cagggctcca agtaatgtaa caattagtaa
      961 tactaaggat atattccgat tgcacatcct tccaatgttt ggtgaatatg caatgaatta
     1021 tctgaattca aatactgaga taatatcaga tgaattaaca aagaaatcga aagaatacgc
     1081 aaatataaaa attatcaaag gctatgttcg ctctatgttt gatattgccg aaatactatg
     1141 ttatatcgaa ttcaaccgaa ctaccaaaat tattcaatgt attaccgcgc ctaagaaaat
     1201 ggctttagaa gaaaaaagga taagggaagg gaatcaagca ttatcatcag aagagctgac
     1261 tgattggata gaagcagtaa acaatgactt aaataatcac ttgcttacac ttcacgatta
     1321 cacacttttc atgcttacct tatatctagg tgatagaaaa agtgagactt atgctctgca
     1381 atggaaatat attgatttta ataaaaatac ggttcgtctc aaatatgctt tagataaata
     1441 tcaaagaaag acacatacaa aaggttggaa agacaccgtt attcaggcac cagaagttgt
     1501 gatgacatta ttaagagaat ggaaatcagc tcaggctgaa caattgttga aactaaaaat
     1561 tatacaaaca cctgaccagt acctctttac ctattccaaa ccttctggag aagtaaattg
     1621 tcctgttcac gctgactact taaactatcg aatcaatgca ataaaaaaaa ggcatcctga
     1681 attggctcaa ttatctccac acaaacttcg tcatacctat gcaactatag ctagggaagg
     1741 tggagcaaat atgaatcaaa tctctaatgc tttaacgcac tcggatattt ctactacaaa
     1801 aatatacgtg aacacacctg acgtagttga taaatctgtt tttgaagcat ttcaaaaagg
     1861 attgaataag tgtgattaac atgtgttctc aaatgtgttc ttattataaa aaaagacact
     1921 ttccaaataa ttgaaaagtg tcctgaaata ttgatataaa aagaatatta acgttttgag
     1981 aattgtgaag ctttacgagc ttttttaaga cctggttttt tacgttcgac catacgtgcg
     2041 tcacgagtaa gtaagccagc acgtttaaga gctaaacgga agtcaggatc aacttctagc
     2101 aatgcacgag cgataccatg acggatagct cctgattgtc cagcgtatcc tccgccgttt
     2161 acgttaacaa atacgtcata tgcgcctttt gtttcagtaa cagcgaaagg ttgattgatt
     2221 acttcacgca agtcagcatg tggaatgtat tcttcaacat cttttttatt tacagtaatt
     2281 ttgccagttc ctggtactag acgtacgcga gcaactgcgt ttttacgacg gcctgtgccg
     2341 atatattgaa cttgtgccaa tgaaattccc tcctattaga ttaagtttgt gatgtctaaa
     2401 acttctggtt gttgtgctgc atgtggatgt tctgctccac cgtatacgtt caattttgta
     2461 aattgtttgc ggcctaaagt gtttttagga agcatacctt ttacagaagt ttcgatcaaa
     2521 cgacgagaat ttttagcacg caattcgcca gcagtgattg atttcaatcc tcctgggtag
     2581 ttgctgtgac ggtaataaat tttgtcagtc gcttttttac ctgttaattt cactttgtct
     2641 gcattgatga cgatgacgaa atcgccagta tccaaatgag gtgtgaaagt tggtttattt
     2701 ttaccacgta gtacagatgc aacaactgtt gaaagacgtc ccaatggaac atcagttgcg
     2761 tctactacat accatttacg ttctacttcg cctggtttgg ccatatatgt tgtacgcacg
     2821 ttattttcct ccaatttaag ttctgatgtt tgacatacac ttcgagtttc cggggctctt
     2881 tgtggggcaa acaataccat ttaacatatt acccgctaat ctagtcaatg tcaacgtttt
     2941 tctgcttgat tgaaaaattt atatgaaact ttacttcttt tctttctttt atataaaagg
     3001 gtcgtttcct gtcaaaatga aaaacaaaaa gaggctgtga caaaagtctt gtcacaaccc
     3061 cttattttga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca caatatccat
     3121 aaaacataag aagctgtttt ttaaaattgt ttcttgtgac tcggagctga acggcaggct
     3181 tacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg
     3241 caaaatatga ggaactattt tgacgtttgt ttctctaata ctcaggtcaa aacgctgatt
     3301 tcacaacctc tttttcggtc aagttcaaca acagttttta cgcgtcgatc tctggcgctt
     3361 tgcccaaatg agcttggatc atccagatac gtttttctgt ggctgttttg aattcgataa
     3421 agatatcttg tgttggatcg tcgccttctt ctccagaaac ttcgattcct ttttggtaaa
     3481 gatctgctag ataacgatag cctgcaacaa gtgtttccaa acgttctgga atagtacgat
     3541 cccagcggcc aatttcatca gggatttttg tgttatctgc aaattcacgc aatgtagaat
     3601 aaggtgaacc gtctaatgtg atcaagcgtt cggatacttc atctaactga gcgttcaaat
     3661 catccatgaa ctcatccatc attgggtgca atgtaaggaa acctggtcct ctcatgtacc
     3721 aatgtgtttg gtgaacaaca acagacattt ggcttagatc tgcaactaat tgatttaata
     3781 cttctttggt ttgttggaat ttcatctttc ttgcctccta acattttctt acacttaaaa
     3841 taatagtaga ctttcaatga aaagtacaac aaaatcattt acctgagaac gattttcagt
     3901 tcgctttttt atccgtaaag ccaatcattt ttcagaatca gctaattctt ctcgcagttg
     3961 agcaaattgc tgataagtct gtgctaaatg cttttgtgtc gaaacaaata atggatagat
     4021 tttctgatat tttttatgat cagtttcttg tactccaact tgctctttca attctatcaa
     4081 ttcctgtgct tcactgatac tttggatctt tccgatgctt ctccagccta aaatcacagc
     4141 tcctaatgcg gatgcttctg tgacagtagg gaaaacaagc gggtaaccca gtacatcagc
     4201 catcatctga cgaaaaatcg gagattgagc aaacccgcct gtagcacgga tctctgtgag
     4261 ctgattttcc tggcaaactc cttttaaaat ccgataaagg ttgagacaga tcccttctag
     4321 aatcgccttc atcatttctt tttctgtatg ttgccgctgt aaaccgataa aactgccaaa
     4381 cgcatctgcg tcccaaagcg gtgctcgctc accaagtaaa tagggatgaa aaatcagacc
     4441 attggcaccc gctggcacaa aagcgatagc cgaaagcagt gcatcatatg gattttctcc
     4501 tttttcttta gcagattgaa cgacttcctg taaaaatgta tgccctgccc aatcaaagat
     4561 gccagcacca ttgctagtcg ctcctcccac tacccaatgt ttctgatcca gaacataaca
     4621 aaacgtctcc atttctggat ggaactgagg ctgagtagta ataaaacgaa gtgctccgct
     4681 cgttcctacg gtcaaagcgg cgatcgtatc accaaaagca cctaacccta gattagaaag
     4741 cggcccgtct gcccctccca aaacaaatga tatatcattt ggcaaaccta gttctgctgc
     4801 tgtttttgat ggtagacctg ttacttgata ggtggaagga aagacttgcg gcaattgctc
     4861 tattcgaatg ttcaagtagt caagcacttc ttctgcccat tggaattcat ggatatcaaa
     4921 atatccagtt cctgaagctg ttccgtagtc gctgatcagt tcgccagaca atctataaaa
     4981 gatatagtct ttgataccga caaaataagc agtttttttg aataattcag gttggctgtt
     5041 ttgcagccac ttgattttgt tcaatggtgt cattggatga atcggcgtcc cagtctgccg
     5101 ataaaattct ttgccaagca cggtatattt cagtttttct gcttcgtccg ctgctcggtt
     5161 atctgcccaa gtgatcattc gagataacgg tgttttctct tcatccaaca atatcaggcc
     5221 atgcatcgca gaggagaagg agacaaattg aatgattgtg tctgaaggca actgtttaat
     5281 tacttctttt ataacggtgc agacaccttg aaagatcaat tcctgatctt gttctgccat
     5341 cccttgtgtt tcttggatca aagggtagcg aaaataagcg gatgctgctt cttgggcttc
     5401 atctgtgaaa gcaactgctt tggtttgtgt ggttccgata tctaatccga ttgaaacgtt
     5461 cattttttca cctcaaattt tatgtatgta caaaaattta gtttaagctt gcgtatcttg
     5521 gttttgaaaa gggggagatg gcgtgttact ttattttatc attggttgct gtatcggatc
     5581 atttttatgt ttaaccgccc agcggattcc gttaggtcac tctattattt atcctagatc
     5641 tcattgtgtc aaatgctgtc gttcgctttc ttggtatgaa ttgattccga ttctctcgat
     5701 catcgtccaa cgatttcgct gccgctattg caggtgtcgg ttgcccttct actatttgtt
     5761 ggcagaagcc ttatgtggtg gtctttttgc ctggttcttt actttttctt ccgcaagaaa
     5821 tttttcgacg ttcatatgga tgctttcggc tttgctattt tcgttgatgg atttatttta
     5881 cttcatggtc cattctcaca cgctattctg ttcttgggca attttatgga ttttttggtt
     5941 gcaggccggc gtcttccaat ggcaaagtgt gctgttaacg tttatcgtag gcggtgtgtg
     6001 ccttcgctat ggtcaatcct atctcggagc tggcgatact ctgttgatgc tttcttggag
     6061 tggtggcttg tctctagaag agttgcttca gatcttgttt cttgctagtc tgtttggaat
     6121 atccgttttt tctgtttact ttatctgtca taaaaaaaag ttggaaaagc tgcctttcat
     6181 tccttttttg agcagtgcgt tgctgattgt ccttcatctt aggtattgaa aaaaggttgt
     6241 gacaaacgac ttgtcacaac ctctgatata tagtgttctc ttaccaaatc cttgtgactt
     6301 ggagctaggc gacaggttca caacctctcc actaaatggt gttcaatcag ctgaccctca
     6361 gtattagctc aaaatgctgg ttttacaacc tctgatatac ggtgttccat aagtagcttt
     6421 ttcggcttct tatttcttga cgccccaatg tcgacgaagg aaacctgcac gtccagaacc
     6481 ttctcgataa agattgtaat ggagcttgtt tttcttgtaa taatcctgat ggtaatcttc
     6541 tgcaggataa aattcgcttc ttggttcgat ttttgtcacg attggcttat cgaatcgccc
     6601 gctgtcttgc aatgcttgtt ttgaacgctc tgcaatattt cgttcttctt catcattata
     6661 gtagatcact ggacgataag aatcaccgcg atcagcgaac tgtcctaatg cgtcagtcgg
     6721 atcggtttgc tgccaataga tttccaccaa ctgatcataa gaaataactt ctggatcata
     6781 ttcgatctct actgcttctg tatgccctgt tgtgcctgtt gttacttgtt cataagttgg
     6841 atttggtaca tgcccgccag tataaccgga tgtcacagaa atgatccctg gctgctcttc
     6901 aaatggtttg accatacacc aaaagcatcc gcctgcgaaa actgcttttt ttgtcatata
     6961 aaatccccct tttttctcat cataccttga tttccaaaag gaggataacg ttttgcttgt
     7021 gacagaaaaa acggaaacta ttatatacgt gatacggctt ctgataaatg ccaaaacgaa
     7081 cgtacttgat caaaaacggt taaatcagga atcacatatt cagcagaagg aagatttatg
     7141 ttttcaccta ttcccaatac atgcatgtta ccagccaaag ctgcttgaca accagcttct
     7201 gaatcttcaa tgaccaagca tgcatttggc ggtacattca attgctgtgc tcctttgaga
     7261 aaaacttctg gatcaggttt tgcttttgat acttgcgtgc cgtcaataag gacatcaaaa
     7321 taacttgtca aattcgtttt ttctaaaatg aggcgtgcat ttttgcttgc tgatcccaat
     7381 ccgatcttga tgtttgtttt tttcaagtac tcaagaacag ctattgcacc aggcaaaacg
     7441 gatgtttcat ccattttttg aatcgcttga agatatagat tgtttttttg ctcagcaaag
     7501 gctgatttct ctttttctga gaatttctgt tccatttttc caaatgacag caatcgctct
     7561 agtgactcgg ttctggatat tcctttcagt tgctcgtttt ctttctcagt gaatggaatg
     7621 gacagctggt tagctaaaac cagccacgct tcgtaatgat aacgagctgt gtctacaaga
     7681 acgccatcta aatcgaatag tgctgctttg attttcatcg attacctcct tgaaaatgaa
     7741 cgacttgaag taaagatgcg atgtttttag ctgtgcgatg aagattatca gtggcttgct
     7801 gtaaagcttc ttgtaaaggc acagctgcag gaaggatcgg aaaaacggcc gaaatgccaa
     7861 catctgctgc tttttcgctt cctttaccaa cacttccgca aatagctatc acaggaacgc
     7921 cctctggtac tcttctagca atcccaactg gcgcttttcc ttttagactt tgtccatcta
     7981 gacgtccttc gcctacgatt acgaggtctg cttgctgtac ccgtttatcg aactccagta
     8041 aatccaatac gtagtcgatg ccagagacta ttttccctga agcaaatgct aacagtcctg
     8101 cacccattcc accaccagca ctactgcccg atgcctcgat tacttgaggg gcaaatctca
     8161 tataaaaatc atgcatggct tggtcagcca ctgctaattc attttctttc aagccttttt
     8221 gtggtccgaa aacaaaagaa gcgccttctt ttccgcataa aagattagta acatcagtaa
     8281 tgacagtgat tttggcttta atcagctcaa taggcacgtt cgttttatca actgtagcaa
     8341 ttttacctag attttctccc agtgcttcta ctttttgatt atcttgatcg taaaaagcat
     8401 aacctagacc ttctgccatg ccgatgccgc catcgttggt actacttccg ccaacgccga
     8461 tgatgatgtg tcgaacacct tgttgcaaca attgtcgtat cagttctcct actcctcgcg
     8521 tggataacct aagtggctgt tgttggttta gtgggacatc tttcaatccg acgatttctg
     8581 ccatttcaaa aatcgcagtt tgaccgtctg aagcgtactg gatttctttg tttttgccaa
     8641 atgcattagt aacgactgct gtttgccgct tcaaccttaa actgtctgct aatgatgaaa
     8701 ccgttccttc tcctccatcg cctataggta gcagatcata ttcagcctga acaaaaactt
     8761 cttgaaaacc tgattgaatg gcttgtgctg cctctaaggc tgtcagactt tctttgaaag
     8821 aatctggcgc aatcagaata cgcattattt tcccctccgt tccaagatca gttctttttc
     8881 cagctggtat tggtctccat tgacaaaaat aggtaatgtt tctttcgtct ctttttttat
     8941 tactatttgt ttttttgtca aaacaaatag caacctgtga tttttccaaa taaaaggaaa
     9001 agaaattttt tcccatgctg aaggaagtct tggagaaata gaaagagttc cattcgttac
     9061 tgtaatacct gaaaaaccaa atataactgc tagccatagt gctcctaatg aagcagcatg
     9121 aattccgtca tctgatgaat gcggatctgg tcctaaatca atcaggcacg cttcttggaa
     9181 taaatgatac gcccattcca tttcttggat acggcaagcc tctatggcgt gaatcgcttt
     9241 actcaacgat gagtcgtgga ttgttttgtt ttcatagtaa tgcaaattct tagaaacgat
     9301 tggttgagaa aaatactcag gtaataagta taacagcatg acaagatctg cttgcttgct
     9361 gacttggagt tcatttactt cgctacgggc ataatcaagt aaaatagact ggctgccagg
     9421 acgttctttg taagtggtca aatcaataac tggcttactg agatacgtgt catcttgcgg
     9481 aatcagctgt tctttgttag gcactggcaa atataactta tctaaaaaat tttgacattc
     9541 tttcaaaaaa agcggatcgc cagcttgata tttaacagct aaggccacat tataatgcgc
     9601 cagatagttc gtatatgcat tattgtcgat atattctgtg tattcatctg gaccaatgac
     9661 tctgtgtatc tctaaatgat catcgacttt tacagctctg ctcagccaaa aagctgctgt
     9721 ttcatgaatg agcgtttttc cctttgtttc taaaaacgat tgatcctctg ttgcttggac
     9781 atactcttga acggcataag caaggtctgc actgatatga tgttctgcgg cagctgaagc
     9841 aacttcttgt cttgttccgg ttttgatatt caatgcagca aatttgggtg tctcttcctg
     9901 accagtgtgt gcgctttccc atgggaaaag tgcaccttga tagtgataat ctcttgcttt
     9961 ttgccgtgcg ccttctaatc gatcataacg gtacagcagc aaattctttg ctacttcagg
    10021 aaactgatat aaaaagaaag gcaggataaa aatttccgta tcccagaaaa catgcccttt
    10081 ataaccttcg ccagttagtc ccttcgcccc tacactaaca cttggatcat tgcggggagt
    10141 cataatctgt aaatggtaag cagcgaagtc catcgctaac tgatcgaaag aagaatcgcc
    10201 ttggatcgtt atccgatgct gatgccaaaa atctgtccaa taagacatcg actgctctaa
    10261 aatttctgta taactaacgc ttttctgggc ttgggcaata cttttcttaa acgggtctgt
    10321 ttgctgaaga gaggtagaga gcacgatgat cttctccagc gtaaaggacg tattttttct
    10381 gatcttttct gaaaaggaag cggtgatccg gcgatttttt gcttggaata cacctgcttt
    10441 attgaattta gctgcaatcc caatatcaat tttactttct gttgtctggt aggatgcact
    10501 gatcaaatcg tcaccaaata ctcgcagttc tttttcaacc aactgctgtg taccaaaatt
    10561 ggtttgctgt ccatcgatac ccgttttcac atctacttgg cagtcttcgg tcaacggctt
    10621 gatgaccact ttcagggctg cctgatggag attattttga tcagcaacac gacaagtaat
    10681 gacttccact ttttttcctt gagcagtttc aatgagcgca tagcgtttaa gctctcccat
    10741 ttctaaatct agtgcccgct cgtatgcaag aactttcatc gtctcaagag aaaaatgttc
    10801 accgtcgatt tttatttccc atctgatgac atctgggaga ttcactaact cggctgattc
    10861 tgctcctaaa ggtcggttat atacaccagc tgcaaacatt ccccttactt gattccgata
    10921 gtcttcctcc tggcttcctc tgataccaaa aaatccattt ccaatggtca tctgactcgc
    10981 atacttattg atatactcta aatcaaactg tttttcttct actttcataa ggtaataact
    11041 tctcctgttt ctgccgaacg atataaattt tccaccagat tctggatgac atatccttgc
    11101 cagccatctg caatcatctg agtctggtca ccgagcgccc gattcacaaa atgagccata
    11161 cttagttcat gtgggtcatt aactggcgtg gtcttttgta aggaaaccag ctttccatct
    11221 acatcttcga atagttcgcc tggatacaga ttagcacctg cctgatcgcc acaaaaagcc
    11281 acattcagtt tgtcttctct gatattcaaa gcaaacgaag tattcagttg gatgatcttg
    11341 ccgttttcta attccagtgt gccaaaaatt gcatcttcta ctgtgaattt gcttggatcc
    11401 catttcccga atttccctga attttttttc gtaccaattt tttggaagct gtacgcccga
    11461 acgcttttta ctttaggaaa tccagtaata tacatcgctg tatcaagcat gtgaatgcca
    11521 taatcgatca aaggcccgcc tccttgaagt tctttgtcga taaacgttcc ccagcctgga
    11581 acaccagacc gacgaagcgc attcgcttct acgtaataaa tatccccgag aacatcaaga
    11641 tgattcttga tcattgttgc ttcgtcagaa aaacgatgct ggaatccata agccaaaatc
    11701 tgcccgtttt tatcggctgt ctctgccatc tttcttgctt gatcagctgt catagctggt
    11761 ggtttctcgc acatcactga agcattagct tcgagggcag ccaatacatg ttcgaagtga
    11821 aagcgattgg gtgtacagac actcacgata tcgggagaaa tctcattcag catttgtgta
    11881 actgatgtat acgcatgaga aaatcctgta cgtcggcaaa agtcttctgc tcgctggctg
    11941 actggatcaa ccacagcggc caattctaag acatctgatc ttttttgata ataagcagca
    12001 tgtacttttt ctgctacttg tccagcgcct atgattgcta ctttcttcat ttatttctcc
    12061 ctcctttgct tatttactta ttgttattct ttctctattc tttctctaaa tcatttaaat
    12121 gacgcgctgt ttccagacag tttttcaagt attgcaatga ttcctgataa attttcactt
    12181 cattttctcc tcgcaaacga cattcatata ccatataacc ttcatactca ttttgaagta
    12241 gcgtttgaaa atggcgagta aaatcaattg cgccagatcc tggttggtag cgatgattat
    12301 ctgctaaatg gatatgaccc acatactctt tttgcgtagt caatgctttt gaaatatcat
    12361 cttcttcaat attcatatga taaaaatcag cgatgatctg cacatgtttc aattgattag
    12421 agtcaatgta atgacgcgca tccgctaaca aattgatcat atggtcttgg tatcggttca
    12481 aaggctccaa ataaataacc gtacctgttt cttcggctac tttatctaaa taacgaagtg
    12541 attcgctgac tgctttatag tctccttctt tgcttcgggg tgaaaccatc ggcggtaatc
    12601 gataagtgaa cattccccaa gctgctggga taacgattcc tttgcctcca acctcgctta
    12661 gtgcacgcaa aatctcagca atttgacgta caccattaag acgccgttct tcgataaagt
    12721 caccgatcca cccatcgtag ccgttacaag ctgtcactac tgggatccct gtttctttga
    12781 ttgcctgttt gactgtgtcg atattttcta ttaacaactt accgtcgatt tcgtattctt
    12841 caaatccaca gtctttgata aatctaaatt tttctaaaat gtctcttggg aaaaatgctt
    12901 gatcttgcgt accgaatttc attgttctcc tcctcggact tgaacgccca ttttgatact
    12961 ttcttctggg tgctggtcta cataacgcat gtaagcttct gcgctttctg caaatggcac
    13021 aactggatca atgatttctt cacaatccaa gtaaccgttc atcaataatt cccaacatgt
    13081 gtcctcgatt cgtttacggt tccatcttgg gtaatctggg tttggttcac tagatgctcg
    13141 agcaaagacg atttttgcat tattgaaatg tgcttcgcga ccaagattaa acccagctgg
    13201 aaaaggttta gcaaaggcaa cataagaaat gatcccgcca tagccgatcc ctcttaaaga
    13261 agcttgtaat gcggcagcac tgccactcgt ttcaataact gaatctactc cacgtttatc
    13321 tgtcaattcc tttagacgaa aaccgacatc tgctttcata ggatctagcg tgtaatcggc
    13381 accgtatttt tctgctaatt ctctacgctg cgctatggga tcgatcccaa cgatggtagt
    13441 agcacctgct tttttagcta attgaatcgc aatttgcccg atagcgccta aaccgaagat
    13501 tgctacagca tcacctgctc gaacatgtgc ttcccggata ccacctaaag cgaattgagc
    13561 aggatcgtaa catactgcat ttttccattg atccaaccga tcaagtttac gtagtttata
    13621 attatcaaca ccgtttgtaa tcagcgtttc acgaatgggt ccataagtgc agactaactc
    13681 tcctttttga tactctgtca ccaaactgcc cgtttcttcg atcgtaccga caatcatatt
    13741 tcctaattga aattcgccaa ataccacacc gcgttggctt ccttcttctc ttggcaagaa
    13801 taggcgccat tcttctgaaa attcttcatc gataaatgga cttaatcctc taaagtcgac
    13861 aatttctgtt cctggttttg gagtagcgaa aattactcgg atacgtactt cgttcttttc
    13921 gatctcacgg tcttcgtaat ctttgaatcc ggctattctt ggttctaatg caactaattt
    13981 tttcatcttt tctttcctct ttcttttctt tttatccttt tacgccgcct tcgactaaat
    14041 cacctttaat aaatcgttca gataacgcat acataatgat gactggtaaa gcggtcacca
    14101 atgaagcggc catcattcgt ccccaaatgt agtctggtgt actgaataat gtgttcaaac
    14161 cgattggtaa agtaaatttt tcggaggtcg acaggaaaat cgaagcaaat aaataatcat
    14221 tccacgaaac cataaagcaa taaacgaaga cagaaatgat tccagaaata gccaatggta
    14281 taatgatccg aataatgatc tggatccggc taagaccatc gatcattgct gcttcttcaa
    14341 tatcagtcgg aatcgtgtca aaataacttt ttagcatgaa tatcgcagta ggaagtgttt
    14401 gaacgaccat cgtgataatc agtgctgtat tggtatcata caaacctaga ccggaaatga
    14461 ttttaaataa tggaacaacg atcaaaatac cggaaaacat ataaacggtg taaaagctag
    14521 cattgatttt cgagcgagct ttgaagcgta gttttgacaa agcgtatgcc ccgctgatcc
    14581 caatcactac tgcagttacg gaggctatca aggagatcac taaactattt ttgaaataca
    14641 tgatgaatgg aaaaatctca gcattgaaaa tatcctgata atgttcaaaa gtccattctt
    14701 tcggaaaaat agtttgtgtc gttgcaattg cttcttgtga atccttgaat gatgtcatca
    14761 gcatgatcaa aaatggaaac aaattgacag ctaatagcaa caaaatcgtc gcgtaaaata
    14821 agattttcct gctcttttta tttttcattt caagtccacc ttctttcgtg agagcaagac
    14881 gaccgctgca ataatgatga acaatagaat cgatatagct gctgcttttc caagatcatt
    14941 gaacgcaaag gctgtctgat acaaataaac gcctaagata ttgactttat tcgtcagtag
    15001 atagacatct gtaaacatat agaacatcca gatagatcgt aaaacaatca cagttgctag
    15061 tactggtttg attgccggca gtgtcacgat cttgaacttc tgccaagcat ttgcaccgtc
    15121 catttcagct gcttcataaa gcgttttgtc gactgtttgc aaaatagcca aaaatgaaat
    15181 aaacgcatac ggatagtatc tccagatagc aaacaaaacg accataaaga agctagcaac
    15241 tggctggtcg aaccacagcg gtgcttcgtt gaaaagctgc aacctatcta ctaacagatc
    15301 attgacgatt ccataactat tattgaacat atatttccaa gcaaaaatca aagaaataga
    15361 tggcgtaaca taacttaata aaatcagtga acgcaccgtt ttcctaaatc tgaacttgcg
    15421 gttaagcaaa atagctacgg ctaatcctaa agctgtactg cctgctacaa ctaagaatgt
    15481 gtaagcaact gttaaaccta aagaacgata aaattcacta tcacttaaaa tttcaagata
    15541 attgcttaat ccgataaaaa cggcgtctga gttaggattc agcggcatat cgaaaaaact
    15601 gatctgaata ttggaaagca ttggaatcag cactaaaaca gctaataaaa gcatgcttgg
    15661 tgctaaaagt cccatggcta attttaagtc agaatgtttc cttggacgat tcattgaaac
    15721 ctccccctga taatctgcca atagctatct acttttttcg aacaccattg gcagaacttc
    15781 ttgttatttc gtattttttt gtgcgtcagc taaagcttga tcgacatctt cgtttccgac
    15841 agttacttgg ttcactgctt ttccgatcac acctgagctt gtgatatctc ccattttcgt
    15901 gaaattcttt tccccgacta gtccaaagac ttgtacttca tcaaaagcac tagcaatctc
    15961 agatggcagc tcgccaaaag acttgatgac ttcattttct ttgtaaccat ccagttcgac
    16021 tacttgttta ttcactggtt gagcaccacc aggagacatc aagacccact tttccatgtt
    16081 tttcggttga gataagaaag ctgtgaatgc ttcagcagcc tctttttgtt tctcgtctag
    16141 accagaagaa atcgtcaatc cagatactgt gccatagaca gcttgatctt tttgtgttgg
    16201 gatagcaaaa ccgatatttt ctgagttccc cgcttcataa acagaaggca aaatgtaagt
    16261 tgaatagatt gccattggtg ctgttccatt catgaaagca tctttgattt ctgtcacatc
    16321 attagagcct ggcatcgtgt attgggataa gttttgataa aacgacaatg cttctctcat
    16381 cttttccgta tcgatcgtta cctcgccttc atcatccaaa acatttgctc catttgacaa
    16441 agcaaattga gagaacgctt gctcactcat tgttccttca gcagtaggca tagcaattcc
    16501 atattgttta ttctctttat ctgtgaaatg ttgagcaatt tttaaaatat catcccaatt
    16561 ttctggttct gaaaaaccag cttctgataa tttttctttg tcataccaaa taccttgaac
    16621 ccagccgcta attggtgcag caatatagct ttttccatct tctgaacgaa caaggttttt
    16681 ggctccgtca taataattat cttctccagc ttcctcgata acgttttgga ctgccgtctg
    16741 atcaatcagt tcatctttat ccatcacttt agcaaaatcc tgactgactt ccattacttc
    16801 tggtaattta ccggaacgag ctaacgtaac gatttttgta ttgaacgctg attcttctac
    16861 cggcacttgc ttgattttga tgtctggatt ttctttttca aaatcagcaa ccagttcatt
    16921 gatcacactt aatcgttcct gttcgacaga agagtgcatg aattgaatcg ttgtctgttg
    16981 tgattcgtta gagctttgac tttcaccaca agcagtaaga agaaatccag tagctagcag
    17041 acaggtagta acaataattt ttttcatttt tttccacctc ttattttatt attttcagcc
    17101 acatatattc atacggctgg aatatcaact ttcgttcttg actgcaatgg ataatttgtc
    17161 cagaaaacag gtctaggtaa tctccttttg gtaaaagata acctgctgat tgattggaaa
    17221 aattatggat aactaagact ttctcttcct tcgcttcgcg aataaaactg ataaaagaat
    17281 ctgctgagtc cagtaagtaa atcaaactat tcggatggaa ggctttttct ttttttcgaa
    17341 tagatagcat ctgggtcaat tcaccatata ctgctgttct taatggatct gtctgcaact
    17401 ctttctcaat atctgctaac aaaaattttt gccgattgat ttttcgattc tctcctgttt
    17461 tttctacgcc tttgtaatca tttcgacttc ccagcacact ttgaatataa attgccggca
    17521 cgcctggtaa cgttaccaaa aatgaatgag cagctaaaaa gcgtttgctt ctccattccg
    17581 tcgtatctgt tggagaactt aacgcatcta gatacgtagc gtttatctca tatggactgg
    17641 tcgttccatc tgggtttttc ttgtagttta ctcgggtacc ctctttcttc agctgttcca
    17701 ctaattcaag gatcgttttt tccggcagaa tacctcgtac cggattcaat ccgataccgt
    17761 catgggaggc taaaaaatta aaaaaagttg tttttttcga tgcgaaatcg atggttttta
    17821 accattcttt caacttactt gtattacccg aacgaatcgc ttgaagggtc aaaggtggta
    17881 gcgaaaactg atagaccatc tgtgcttcat tctcgccatt cccaaaataa ctgatattat
    17941 ctttgtgtgg cacatttgtc tcagtgatca ataccgttcc tggagctgct tcatcagcga
    18001 ttgcacggaa taattggatg attttatgcg tttctggcag gtgtatacaa gaagttccta
    18061 gttctttcca taaaaatccc acagcgtcca agcgaataaa tgaagcgcct ttttctaagt
    18121 aaaacaaaag aatatcgatc attttcaata aaacttctgg atttttaaaa ttcaagtcta
    18181 cttgatcttc gctaaaagtc gtccatacgt gtttttcttt cccgtttttg tcaatgaacg
    18241 ttgtaagtaa cgggctggtc cttggtctag tcacgctgct tgtatctgta tccagtggca
    18301 aatcaataaa aaaatctttg tacgtatcat tcaagtttaa atattcctga aaccaagtac
    18361 tttcggcaga catatgattg cagacaaagt cgaacattaa ccgtgtttcg cttctcattt
    18421 cttctatatc ttcccatgtg cccaaacggt cgttgattgc ctgataattt acaacggaga
    18481 aaccatcatc agatgtatat gggaaaaatg gcaagaaatg tacaactcta aatgtatctg
    18541 ccaaatactt ttgataaaat ttcttaaatg tacgcaaagt tggttgctgc tcttcaaaaa
    18601 attgatcccc ataagtaatc aagaaaatat cttcttcgtc ccaatgcagt ttgcctgcct
    18661 ggcctttctt ctgctgataa acactgactt tttgttcaat catagaggcg gttttctcag
    18721 acaaatctga gtcgcgataa atcgcttgta aaagttttgt atacatcgga ttcccctttc
    18781 ttttttggga gcgttcccaa cgatttgatc atattatgga aaacgcttac tgtcaatagc
    18841 atttttttaa ataattttga tatacacaaa aatacaaata agaaagctta tgtatcaata
    18901 attaatagct tcttaaaaaa atatggtctc tcatgatcat gcaagctatt cctaaatttt
    18961 caaaatcata gacaaaaaag aatgtctatt gtactatgga attattctaa aacgctccca
    19021 aaaaggaggt gcatataaaa ttgaaaccta ctatttatga cattgcgaaa aaagctaatg
    19081 tctcaaaatc aactgtctcc agagtattaa ataaccagcc aaatatctct gataaagcaa
    19141 aagcaagtgt acttcaagca atcaaagaat tgaattatga accgagcaaa attgcaagag
    19201 gactgtcagg aaatggtttt gacgcgattc tagcattatc tcatcgtact tctcatacga
    19261 cctatggcaa tccttttttt tcagatgtta ttcaatccat ttctgccgtt tctgaacaaa
    19321 ataacttcga cttgattcta caaacggcaa aaacaccaga agaagagcta gaaaaatgcg
    19381 cgaaaaagat ccaagaaaaa atgatccgag gaattattat tttaagctct acgatggatg
    19441 aaacacttct ttttgaattg gatcaataca atattcctat tgtggtaatc ggaaaagtcg
    19501 gaaacgacta cacgaacatc tactcagtgg acacggataa tttccaggat agttacgatt
    19561 tggtggaaac actgatcaag aatggacacc gtcatatcgg ctgtctccat gctccgacag
    19621 aagtttatgt atccaatgat cgtctcaatg gttaccgtca atgcttattt gatcaccacc
    19681 tagatatccg taatgaatgg attatagacg gcggctttcg cttagaagat agcatggcag
    19741 caatgaatga tttaatgaag ctacgagaaa gaccaactgc gattttttct accgatgcat
    19801 taaaatcatt gagtatttac aaagtggcag aacaatctgg aaagcagata cctgatgatc
    19861 tttcgatcgc aggattcagt gatcccttat tgtcctccct attttcgcca cagttgactc
    19921 ggatagacat tccgacaaat cagttaggaa tcaaagccac agaactttta ttccaactaa
    19981 tccaccacca ataccctgaa gaaaaaagaa tcattctttc aaccgagatt actctcacag
    20041 attcgatcca acaacaagaa atgtagaaca gaattataaa agaacagaaa cactgaggat
    20101 aagtcagtta ctctgttctt ttttcaatca ctcatccctc gttaccccct cccggtattt
    20161 tagcgaattt ttcttatttt ccatctgttt tagatagctg tggataacta taatgtttgt
    20221 gttaaactat tttttccaca gggaagaaaa aactattttt aaccacgttg tgtataagtt
    20281 tttatagttc gtttttttaa acacatttac ccttatttca atccatgtta tttggggata
    20341 cttatctcta aaacataaat agaataacag acatgtctac tggttttgtt ttattcgagg
    20401 aattattcaa taatagctag caaaatatta aaaataaccg ctctatcttc ccatcaattt
    20461 attttgcatt caaaaaatga tctatcttta cttttgttga ttgacttctc ttggaagatg
    20521 atcgttccga taccttaatt ggatacgaac cgatgttgtt aagatccttt cccacttttc
    20581 ttctagttag acaaattatt ttagacaaac caaaaaagag gttgtgacaa taatttgtca
    20641 caacctcttt tttaaaatat ctattttgtt tgattgttca ttgcgtcttc tgcagccatt
    20701 tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg ctacaccgac ttcttttggt
    20761 tccatgtcac gataacgttg cataccagta ccagctggga tgatcttacc tataataaca
    20821 ttttctttca aaccaagaag aggatctttc ttaccgcgga tcgcagcatc cgtcaagaca
    20881 cgagttgttt cttggaatga tgcagcagat aagaaactat ttgtttctaa tgatgctttt
    20941 gtgataccta gtaagacagg tcgagaagtt gcaggaacgc ctcctgagac tagcgtattg
    21001 tagttttgat ctttaaagtc tgcgatgtct aataatgtac ctggtaagat atctgtatca
    21061 cctggatcca ttacacgaac tttacgcagc atttgacgaa ccattacttc gatatgcttg
    21121 tcgccgattt ctaccccttg catacggtaa acacgttgta cttcacgaag caagtagttt
    21181 tcaacagata atacatcgcg aacttgcaac aattgttttg gatcgattga tccttctgtc
    21241 aatggtgcac cacggtggat catatcgcct tctgctactt tcatacgtgc tgtataagga
    21301 acagtatacg tacgtgtatc tgtttttcct ttgattgtta cttctttggt acgtgttgca
    21361 ggatcttctg agatatcgat gacatcccct gttacttctg tgatcactgc ttgccctttc
    21421 ggattacgtg cttcaaagat ttcttggaca cgaggaagac cttgagtgat atcgtctccg
    21481 gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag taccaggttc accgattgat
    21541 tgagcggcga tcgtaccaac tgcttctccg acttcaacgt cagagccagt tgcaaggttg
    21601 cgtccgtaac aatgtttgca gacaccatgt tttgtattac atgtgaagac agaacggatt
    21661 gtcactgttt cgacacctgc atcaacgatt tgttttgcga tgtcttcgga aatcaattgg
    21721 tttcctgcaa tgatcaattc atttgtttca ggatgacgta cttctttacg ggtgtaacgt
    21781 cccactaaac gatcttctaa tgattcgatg atttcatttc cctcgcggat ggcttggatt
    21841 tcaagacctc ggtcagtacc gcaatcttct tcacggatga ttacatcttg ggccacgtca
    21901 accagacgac gagtcaagta acctgaatcg gcagtcttca aggctgtatc ggtcatccct
    21961 ttacgagccc cgtgagttga gataaacatt tccaagacgg atagtccttc acggaagttc
    22021 gaaatgattg gcaattccat gattcgacca ttaggagcgg ccatcaaacc acgcattccg
    22081 gcaagctgtg taaagttgga aatgttacca cgagctccag aatcagacat catgaagatt
    22141 ggatttttcg cttccaaacc ttccatcagt ttttgttgga tgctgtcttt tgcagcattc
    22201 caaacagcaa tgacacgttc ataacgttcg tcatctgtga tcagaccacg acggaattgt
    22261 tttgtaatag tttcaacttg tttatgggca ttttcaatga tttcttgttt ttcattcagt
    22321 acactgatgt cagcgatccc tactgtgata ccggcatgtg tcgaatgttt gtaccctaag
    22381 tctttcatac ggtcaagcat cttagaagtt tctgttactt tgaatcgttt gaagacttct
    22441 gcgatgatgt ttcctaagtt tttcttcttg aacggtaata ctaattcttg ctctttgata
    22501 tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt ccggtgtttg gacggtcaag
    22561 ttgaagtctg ttggttcatt cagataaggg aattcaggag gcatgatctc gttgaagatg
    22621 atcttaccaa ctgttgtgat catcgtacgt tctttttgcc attcagtaaa tggtttttca
    22681 cctaatgagt tagggttcac accgatacgt gaatgtaaat gaacatatcc gttgcgccat
    22741 gcgataacgg cttcgttttg atctctgaag accattcctt cgccttcgcg tccttcttct
    22801 tccattgtca agtagtagtt acctagaacc atatcttggg aaggagtaac aactggttta
    22861 ccatctttag ggttcaagat gttttgagca gctaacatca acatacgtgc ttctgcttgt
    22921 gcttcttcat tcaatggaac gtggacagcc atttggtctc catcgaaatc ggcattataa
    22981 gcttcacata caagtggatg caaacggatt gcgcgtcctt gtactaaaac tggttcgaat
    23041 gcttggatac caagtcggtg aagcgtaggt gcccggttca gcaatactgg gtgttccttg
    23101 attacgtctt ctaaaacatc ccatacttcg tcttcttgac gttcgatttt acgtttcgca
    23161 tttttgatgt ttgaagctag ttcgcgttga acaagctcac gcatcacgaa tggtttgaat
    23221 aattcaatgg ccatttcttt tggaagtcca cattggtaca tcttcaagaa aggtccaaca
    23281 acgataacgg aacgacctga gtagtcgaca cgtttaccta gcaagttttg acggaaacga
    23341 ccttgtttcc ctttcaacat atgagaaaga gatttcaatg ggcggttacc tggtccagta
    23401 actggacgtc cgcgacgacc gttatcgatc aatgcatcta ctgcttcttg cagcatacgt
    23461 ttttcatttt gaacgatgat tccaggtgca ttcaagtcta acaaacgttt caaacggtta
    23521 ttacggttga ttacacgacg gtaaagatca ttcaagtcac ttgttgcaaa acgtccacct
    23581 tctaattgaa ccattgggcg taaatctggt ggaataacag ggataacgtc cataaccatc
    23641 cagcttggtt gatttcctga agcgcggaat gcttctaaaa tatccaaacg gcggattgca
    23701 cgtgtacgtt tttgtccttg tgcagatttc aattcttctt tcaattcggc tgcttctgca
    23761 tctagatcaa cattatcaag taattgttta atcgcttctg cacccatagc agcttggaat
    23821 tcttgtccat attgttcacg tttttcgcga tattcgcgtt ctgtcaacaa ttgttttttc
    23881 tctaaattcg tatttcctgg ttcgatgact acatatgaag caaaataaat gatttcttcc
    23941 aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg aaggaatccc tttaaagtac
    24001 cagatatgtg aaacaggagc tgctaattca atatgtccca tgcgttcacg gcgaactttc
    24061 gaacgagtaa cttccactcc gcatcgatca caaacgattc ctttataacg gatacgtttg
    24121 tattttccgc aggcacattc ccagtctttt gtaggaccaa aaatccgctc acagaacaac
    24181 ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct caggtttttt tacttcaccg
    24241 taagaccagc ttctgatttt ttcgggagaa gccaaaccta tttgcatact ttcgaattta
    24301 tttacatcga tcaaaagggg ttccctccat ttcatttaat ggcaaaccgc aagacggctg
    24361 atcttcgacc gccttgcagc tcatagaccg gattagtcta aattatcttc tgcggtttca
    24421 aagttttgga tgacatcgtc tttttcatct tccacttgtt cagcagcttt cttttctaat
    24481 tctttggcag tttgctgttc ggcaaatttt gtcaaagcat caacagtgat caaatcgtcg
    24541 tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt cgatatccaa gacacgcatg
    24601 tccagaccta gtgattgcaa ttctttcacc aatacgcgga atgattccgg tacacctggt
    24661 tttggaattg gttcaccctt gacgattgct tcgtatgttt tcacacgtcc gactacgtca
    24721 tctgatttgt atgtcaagat ttcttgtaaa gtataagccg caccgtaagc ttccagtgcc
    24781 catacttcca tttcaccaaa acgttgtcca ccaaattgag ctttacctcc caatggttgt
    24841 tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca atttgtcatc gaccatgtga
    24901 gcaagtttga tcatgtacat cacaccgaca gacacacgtc catcaaatgg ttcacctgta
    24961 cgtccgtcgt acagaacagt tttcgcatcg ctggccatac cggcttcacg aactgtttcc
    25021 catacgtctt catcacttgc accatcaaat actggcgttg ctacgtggat acctaactga
    25081 cgtgcagcca tacccaagtg taattccaat acttgcccga tgttcatacg tgaaggcacc
    25141 cctaaaggat tcagcatgat atcgattggt gttccatcag gtaggaatgg catatcttct
    25201 tccggcataa tacgggatac aaccccttta tttccgtgac gaccggccat tttatcccct
    25261 tcatggattt tacgtttttg aacgatataa acacgaacta acatgtttac gcctggtgac
    25321 aattcatctc cagcttcacg agtaaagatt ttcacatcgt gaacgatacc gccgccgcca
    25381 tgtggtacac gtaatgatgt atcgcggact tcacgagctt tttcgccaaa gatcgcatgc
    25441 aatagacgtt cttctgcaga taattctgtt accccttttg gcgtgacttt ccctacaagc
    25501 aaatcgccat cttggacttc tgcaccaata cggatgatac ccatttcgtc taaatctttt
    25561 aatgcgtctt ccccgacatt agggatttcg cgtgtgattt cttcaggtcc taattttgta
    25621 tcgcgggctt ctgattcata ttcttcaata tggatagaag tgtatacgtc atcttttacc
    25681 aaacgacggc tcatgatgat cgcatcttca tagttgtacc cttcccaagt catgaaacca
    25741 actaaaacgt tttggcctag agccatttcg ccttgttcca tagatggtcc gtctgccaga
    25801 gtatcccctt tttcgacttt ctcacccaaa tgaacgattg gacgttggtt gtagcttgtt
    25861 ccggagtttg aacgacggaa ttttgttaca tcgtatttat ctaatgcacc gttgtcgcgg
    25921 cgtacgcgga tttcagaagc atcaacgtat tcaacgacac cgtcatgttt acataacaaa
    25981 gcagctcctg agtcatgcgc tgatttgtat tccataccag taccaaccca tggtgcttga
    26041 ggattgatca acggcactgc ttgacgctgc atgttggcac ccatcaaggc acggttggag
    26101 tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg caactacttg tttaggtgat
    26161 acgtccatgt agtctacttt atcaatagat acttctaagt tttcactttg agcacgagcc
    26221 ataacaacgt cttgagcaaa tgttccatct tcattcaatg ggctgtttgc ttgggctacg
    26281 atgtaatggt cttcgatatc agctgtcaag tagtcgattt ggtcagtaac acgacctgtt
    26341 tcacggtcta cacgacgata aggtgtttcg ataaatccga atttatttac tttcgcataa
    26401 ctggacaaac tattgatcaa cccgatgttt gggccttcag gagtttcgat cggacacata
    26461 cggccatagt gggagtagtg aacgtcacga acttcataac cggcgcggtc acgagtcaat
    26521 ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca attcgccaag agggtttgtt
    26581 tgatccatga actgtgacaa ctgagaagaa ccaaagaatt ctttgatact tgccacaact
    26641 ggacggatat tgatcaattg ttgaggtgtc aatgtttcag tatcttggat cgacattctt
    26701 tcacgaacca cacgttccat acgtgctagc ccgatacgga attggttttg taaaagttca
    26761 ccgactgaac ggatacgacg atttcctaag tggtcgatat catcgacatt gccgatacct
    26821 tccatcaagt tcaagaaata gctcattgaa gcaatgatgt cggctggacg aaccgttttt
    26881 acagctgctt ctggatagcc gtttccgatc acgtttactt cacgttcagg gtctcttggt
    26941 gaaaatactt tgatgacttg gacagtcatt ggatctgtga ccacaccgtc ttctgatgga
    27001 tagtaagtca ctgaatttag gccattatca atgaaaggag ctagtgtttc catgacttgg
    27061 tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg tttctggatc aactaatgtt
    27121 tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta attttttgtt tactttgtaa
    27181 cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga aacgagcatt cagcaagtta
    27241 cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac gttcgtaaac gtctttcaaa
    27301 ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt ctttttcgac tgtattgcgt
    27361 aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat cggaaccaaa tcctaacgca
    27421 cgaactaata cggttaaagg aatcttacgt gtacggtcga tacgtacata agagatgtct
    27481 tttgcatcag tttccatttc tagccaagct ccacggttag ggataacagt agaaccgaat
    27541 ccttcttttc cgtttttgtc gacttttcca tggaaataga cacctggtga acggactaac
    27601 tgagaaacga ttactcgctc tgccccgttg atgatgaatg ttccttgttc tgtcattaat
    27661 gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc cagtctcgcg gtttgtcaaa
    27721 cgcaatgtta catgtaatgg tgcagagtag tttgcatcat gcgcacgagc ttctgctact
    27781 gtatactttg gttctttcaa ttcataatct acaaattcca aagatagatt gccattgaag
    27841 tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc cctcatctaa gaaccattgg
    27901 taagagtctg tttgaatttc gatcaaattt ggtaattcca acacttcact gattcttgcg
    27961 aaacttctac gttcgcgatg ttttccgtat tttactacgt gtccagccaa gctcttcacc
    28021 cctctaaaat gtcattcaaa aagactaccc gatcaattgt tacaataata tttcaaatat
    28081 tatttttagg taacaacaga cattttttgc ggcttttagg caaaaaaaaa ccaaaaatag
    28141 aacattctct tgaaacctta cttcgtgagc ttctactttt gtttccttat attaaagccg
    28201 aaacggacaa tatttcgttt ctgccttttc ttcatcagat agtttttgca acttcatata
    28261 ctacacttat tttctataaa agtcaacact tcctggcgtt tcttctgctt tttcgcaaca
    28321 ttttcttaat gttttcaatt ttatgttttc aattatctga caattcttaa ttgacagacc
    28381 tttttatctc ggttatactt tagaaaattc attttgtgga ggaacttcta tgcgaaccaa
    28441 catcaaccaa atcgcaccaa cacaactgaa taacttttac tttagctact ttagataggt
    28501 ttgtattcat catgatggat acaaacgtta aacgacagtt tgtatctatg atggctaaag
    28561 aactttgtat tgcagcaaaa agccacgtag acaaaatgct aagtggctga gcagtgaaaa
    28621 tacaaagtaa aagtatcaag cattctactt tttcaattta ttttccgact tttcaatcca
    28681 tttggacatt ttgtctgaat ggattttttg tttcccaaaa ttaaggagga aaaagaaatg
    28741 aaaaaaaata taatcatcgg attaggcgct gccctgctac tagctttagg aggttgcgca
    28801 caaaaagaca attccgcaga ggcatcagat ggacaaaaaa caattggcgt attgcaactt
    28861 gtacaacata actcgttaga tgctgcctat aaaggattca aagaaggatt agcagaagaa
    28921 gggtataaag aaggcgataa tctaacaatc gattatcaaa atgcccaaaa caatcaagac
    28981 aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc ctgatttgct acttggaatc
    29041 gccactccag cagcacaatc tttattaaat gaaacaacca ctatcccgat gactgtcaca
    29101 gcggtcacag acttggaaca agcccattta gtcacatcca atgaaaaacc tggcggcaat
    29161 gtgactggca cgacagatat ggtgccaatc gaaaaacaaa tcggcttact gttaaatatc
    29221 gtgccagacg ccaaaacaat cggcatcatg tataacaacg gtgaagcaaa ctctaagatc
    29281 caagctgatt tggcagaaaa agaattgaaa aaagcgggcg tcaaagtcaa agtactgaca
    29341 gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc tagctaaaga tgtagatgga
    29401 atctatattc ctactgataa cacgttcgca agtgcggcaa gtgttgtcgg tgaagtcgca
    29461 aaagaaacaa aaacacctgt cgttgctggc gcagttgatc aagtcaaaga cggcggtcta
    29521 gcaacagtag gaatcgacta cgaaagcttg ggtaaacaaa cagggatcat ggcagcaaaa
    29581 atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa cagcgaatga tttggaactg
    29641 tatgtcaata aagacatggc caaagcatta ggcatcaatc cagacagcat caaagaagct
    29701 gaataaaaca caggaggatt ctcatgacaa atttgctctc tcttttctta tcatctgcat
    29761 cgcaaggggt cttatgggcc ctacttgcga ttggtgtata cgtcactttc cgtatcttgg
    29821 acattgccga cttgactgct gaaggaagct ttcctttagg tgcagggatc gcggcagtaa
    29881 gtattacaaa tggctatcat ccattgacag cttgtcttct aggcttttta gggggtgcgg
    29941 cagcagggct tgtttccgga ctgcttcaca caaaattgaa aattcccgcc cttcttgcag
    30001 ggatcatcac catgactggt ttatattctg tcacatcccg tgtgatgggt gcgccgaaca
    30061 tctctttatt gggtcagcca acgatctttc attgggtcga gtcattcggc ataacaaaag
    30121 acaacgctgt actgatcgtc ggtctgcttt ttgctttgat cgttgtatgt ttacttgttt
    30181 tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac tggcgataat ctggcaatga
    30241 gcgaagcaaa cgggatcaac acagataata tgaagatcat cggttatatg atttctaatg
    30301 gatgcattgc tctttctggc tccttgcttg cgcaaaacaa cggctttgct gatttaaatt
    30361 ctggtattgg aacgatcgta attggacttg cttctattat catcgcagaa gttttgttac
    30421 gtaaccagcc actatttctc cgtctcttga ctgtcgtttt aggtgcggtc atttatcgtt
    30481 ttattttagc gttagtcttc gaactgaatg ttgaaccaag tgattcgaaa cttgcttctg
    30541 ctttcgtgct agtgatctgt ctttctttcc cgcagatcca gcaaaaattc catttacctc
    30601 aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta acattggaaa atctccatca
    30661 tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg aaaggcatta atctttcctt
    30721 agatcccggc gacttcgtga cgatcatcgg tggaaatggg gctggtaaat ctactttgct
    30781 gaacagcatc gccggtacat tttctgtcga tcagggtcaa attttattga atggaaaaaa
    30841 tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc agtcgcgtat tccaagatcc
    30901 taaactaggg acagccgttc gtttaacggt agaagaaaat cttgctcttg ctatgaaacg
    30961 aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa gaccgaacat ttttcaaaga
    31021 ccagctggtt cggctaaatt tggggttgga aaatcgttta tcctcagaaa tcggtttgct
    31081 gtctggagga caacgtcaag ctattacgtt attgatggcc acattacagc gtccagaact
    31141 gatcttatta gatgaacaca ctgcagcgct tgaccctgct acttccaaaa cggtgatgga
    31201 attgacagaa aagctgatca ctgagcagaa tctcactgct tttatggtca ctcatgacat
    31261 ggaagacgcg atccgctatg ggagtcgact gatcatgctg catcaaggaa ggatcgtcgt
    31321 agatatccag caagaggaaa aacagcagct cacagtgccc caattattag cgttgttcaa
    31381 acaaaacagc ggcacggcat taaaagacga tcaagtactt ttatcttcat aatcagaaag
    31441 aggatgggac aaaaaatgaa cgcccaccct cttctttttt ctctttcctt ctttcgtttt
    31501 tgctttatgt atcaaccgtt gtttcttcga aaactatttt gttctctcct tcttcctgat
    31561 agaatggatg ggaaaggagc ttgatgccat gacaacgaaa gacaaggtac ttcaattatt
    31621 gaaaaagaca ccagattttc tttccggaga aaagttggca cagcaactgg aagtttcccg
    31681 gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca ggctatcgtt ttgagcacgg
    31741 gcctacaggg tatcgttacc tgccctctga cgtactagat gcaaaagaaa tccagcaaga
    31801 gctacggtcg tttattcctg atttgaaagt acaaacgatg ttgacttctg aatctacgat
    31861 gaaagatgcc aaactggcag cagctcaagg agattctgtt tcgaagttat ttgttgctga
    31921 tacacaagaa caagctcgtg gacgttttgg acggcctttt ttcgctgaac ctggacgagg
    31981 aatctacatg agtctcttgc tccatccaga caaacggttt gatgaattgc ctcaatatac
    32041 gatcatcgca gccagtgcct gtgtgaaagc cattgaatct gtaaccggtc gttctgcttc
    32101 gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa gtctgtggga ttttatctga
    32161 agcagtcagt gatatggaaa gcggcaggat cagtcatatc atcattggga tcgggcttaa
    32221 tttttctgtt cctcaaaatc attttccagc tgatctccaa gaaaaagcca tctctgtctt
    32281 tcccgacgga caagctagtg tcacaaggaa tcagctaatc agcaaaatct ggcaagaatt
    32341 gttcacttat ctttctcacc cagaaaaagc gttggacttc tataaagaaa aatcattcgt
    32401 attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact tatgtcggtc ttgcaaaaga
    32461 aatcggtgat catggcgaac tgatcgtcca aacaaatgaa cagaagatga gtttgtcttc
    32521 tggcgaaatc agtctcacat ctatccgtac gcaataatta tcagatttca caaaaggaca
    32581 cctgatctca tacagtgtcc ttttttctga attctcttca aaagactctc cttattctat
    32641 cagctgacag tttctctatc ctctagaaaa aggtattcta aaaatatcta taaagcatgg
    32701 tacactttct ttaaatgaat caatgcaaaa aacaagagag tcatcagttt acacgatcaa
    32761 aagagatagc aggccgcttc ctttttcaat cttatagata gaccatgggt aacgataaaa
    32821 acaaaaaaga acttgcgact tattttgccg taagttcttt tttagctagt gccttaatct
    32881 cttaatccca acaaacgtct cgcatagatc acctgaccag agtgcatagc agcatcatca
    32941 ataatagaaa cgagacgcat tcctcgtgtg actggtggtg tccagttctc gtcaattact
    33001 tcatctagct cggaaggttc cagccttctt aagtaagaaa tagcaaaatc agcagctcct
    33061 cgcaagtatc ctagcagatc ttctatttga ttagcttgga tctgctttgc ttgttccaga
    33121 ctatgcttcc aatcttgttc gttatctgct atcgtcatcg gaaaccggct ttcccagtct
    33181 tgactatgcc aaattggaga agttcctgct aaatcagcaa tctggaaatc taattctcga
    33241 gcggtatgcc aagccagcca agtcattgat ttgatctgcg gggctctatc ttccactgga
    33301 aaacgattgg cctcctcaat agagacgcct tcaaacaaac gttcaaaccg atcgtatgcc
    33361 cgatccacac tctcgcatag catatctaaa taatccattt attttcctcc tttttattta
    33421 tcctacttac tttttcctaa ttctaggata cctacttata tgttactaca cacccttatt
    33481 ttttacactt taacagtcac catcatcaat agtttggaga ttgaaagaaa aagatagtgt
    33541 cgaactatca attgaacaag aagcaacaat atgaacgttt cattaatatt ctttaaataa
    33601 acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa aagagtataa taggaaaatg
    33661 tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa gtttactagt cctagttgct
    33721 ttgttgttca atatcttttc gtatggtgtg gccgtttcag cggaaacata cattcctaaa
    33781 gaccctggga atatttataa tgatctaact ggtaactatg gcttgttagg aattgctagt
    33841 caattccacg tatttgctaa aaataagaca actgtgaatg cacatacaga cggaaatata
    33901 gcaacacatg aacttgatgc aaaaaataat tttggtacag agcttcatga aggtttattg
    33961 aaccaagaaa taaattatat acaaaaaact gattctttcg ctaattcttc aggcatacct
    34021 acaggtagca caagaatgaa taaatttgtc gttggcagta acgtggatgt agcacttcaa
    34081 gatggtcatc ctacagtcaa tggatccagg cttgatcact taactccaga tgatttttat
    34141 aaagatcgtg ctgggcatga atatattgat ttcgagcagg aatttgctaa attgaatgta
    34201 gcagcaaatg atcttgcaac gatcactcca gctaaaacat atactgctgc agattttcca
    34261 gacatgaaca atcggacaat cgatttaact gatttatcag ataccgggtt tttattggta
    34321 aatatcgatg cagaagtgtt aacgatgaat acgccacttc aaatcattaa tccaaatgat
    34381 caagtagttg tatttaatgt catcaacagt tcttctgcat tgaacgtaca gtcaccaatc
    34441 aaatacaatg atcgttcaaa tcatgaaaca gaagattttt cggatgcgaa tatctcttgg
    34501 aattttggaa atgagatgac tgatttgaca atcagtgctc cttttcaagg aacaatcctt
    34561 gcgcctaatg ctactatccg cattactcaa aaccaagacg gaacgattat aggaacaaat
    34621 gtgattttag atgcagctac caatcgttgg gatcctaacg aaatatttat cagcgacaat
    34681 ggtacagata cgacagacac aagcgacagt acagatacat ctaccacaag tgatagctca
    34741 gattcatcaa cttcaagcga cagtacagat actacctcta ccacaagtga tagcacggat
    34801 acatcagctt caagcgacag t
//
LOCUS       DACDRN010000021        34183 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDRN010000021 DACDRN010000000
VERSION     DACDRN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.021"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            337..2502
                     /gene="acm"
                     /locus_tag="IWJ80_001276"
     CDS             337..2502
                     /gene="acm"
                     /locus_tag="IWJ80_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP9461018.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2774..3259)
                     /locus_tag="IWJ80_001277"
     CDS             complement(2774..3259)
                     /locus_tag="IWJ80_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP9461019.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3739..4380
                     /locus_tag="IWJ80_001278"
     CDS             3739..4380
                     /locus_tag="IWJ80_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9461020.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4399..5034
                     /locus_tag="IWJ80_001279"
     CDS             4399..5034
                     /locus_tag="IWJ80_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9461021.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            5052..5867
                     /locus_tag="IWJ80_001280"
     CDS             5052..5867
                     /locus_tag="IWJ80_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9461022.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6186..7622
                     /locus_tag="IWJ80_001281"
     CDS             6186..7622
                     /locus_tag="IWJ80_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9461023.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7811..10258
                     /locus_tag="IWJ80_001282"
     CDS             7811..10258
                     /locus_tag="IWJ80_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9461024.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10488..11555
                     /gene="rlmN"
                     /locus_tag="IWJ80_001283"
     CDS             10488..11555
                     /gene="rlmN"
                     /locus_tag="IWJ80_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9461025.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11748..12851)
                     /locus_tag="IWJ80_001284"
     CDS             complement(11748..12851)
                     /locus_tag="IWJ80_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9461026.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13404..13952
                     /gene="aac(6')"
                     /locus_tag="IWJ80_001285"
     CDS             13404..13952
                     /gene="aac(6')"
                     /locus_tag="IWJ80_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9461027.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            14498..16159
                     /locus_tag="IWJ80_001286"
     CDS             14498..16159
                     /locus_tag="IWJ80_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9461028.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(16229..16576)
                     /locus_tag="IWJ80_001287"
     CDS             complement(16229..16576)
                     /locus_tag="IWJ80_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP9461029.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            16660..17601
                     /locus_tag="IWJ80_001288"
     CDS             16660..17601
                     /locus_tag="IWJ80_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9461030.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17602..18645
                     /locus_tag="IWJ80_001289"
     CDS             17602..18645
                     /locus_tag="IWJ80_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9461031.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18651..19721
                     /locus_tag="IWJ80_001290"
     CDS             18651..19721
                     /locus_tag="IWJ80_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9461032.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19711..20664
                     /locus_tag="IWJ80_001291"
     CDS             19711..20664
                     /locus_tag="IWJ80_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9461033.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20676..21686
                     /locus_tag="IWJ80_001292"
     CDS             20676..21686
                     /locus_tag="IWJ80_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9461034.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(21971..22792)
                     /locus_tag="IWJ80_001293"
     CDS             complement(21971..22792)
                     /locus_tag="IWJ80_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9461035.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(22889..23167)
                     /locus_tag="IWJ80_001294"
     CDS             complement(22889..23167)
                     /locus_tag="IWJ80_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461036.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            23435..25444
                     /gene="metG"
                     /locus_tag="IWJ80_001295"
     CDS             23435..25444
                     /gene="metG"
                     /locus_tag="IWJ80_001295"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP9461037.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(25923..26510)
                     /locus_tag="IWJ80_001296"
     CDS             complement(25923..26510)
                     /locus_tag="IWJ80_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461038.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            26685..28007
                     /locus_tag="IWJ80_001297"
     CDS             26685..28007
                     /locus_tag="IWJ80_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9461039.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            28162..28938
                     /locus_tag="IWJ80_001298"
     CDS             28162..28938
                     /locus_tag="IWJ80_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9461040.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            28931..29500
                     /gene="rnmV"
                     /locus_tag="IWJ80_001299"
     CDS             28931..29500
                     /gene="rnmV"
                     /locus_tag="IWJ80_001299"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP9461041.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            29493..30377
                     /gene="rsmA"
                     /locus_tag="IWJ80_001300"
     CDS             29493..30377
                     /gene="rsmA"
                     /locus_tag="IWJ80_001300"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP9461042.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            30828..31457
                     /locus_tag="IWJ80_001301"
     CDS             30828..31457
                     /locus_tag="IWJ80_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461043.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(31581..32690)
                     /gene="ugpC"
                     /locus_tag="IWJ80_001302"
     CDS             complement(31581..32690)
                     /gene="ugpC"
                     /locus_tag="IWJ80_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP9461044.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            32899..33321
                     /gene="mgsA"
                     /locus_tag="IWJ80_001303"
     CDS             32899..33321
                     /gene="mgsA"
                     /locus_tag="IWJ80_001303"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP9461045.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            33644..34180
                     /locus_tag="IWJ80_001304"
     CDS             33644..34180
                     /locus_tag="IWJ80_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9461046.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT        11390 a         5916 c         7068 g         9809 t
ORIGIN      
        1 tagtataaac taataatgtt aattaaaagt gtattttatg aaactatcgt tatttattaa
       61 tgttgcattt ttgtaagtta aaaattggaa atatagattg aaaaaatgaa agcaataagt
      121 aaaataaaat gaatatatgc gaaaaaataa tttacattag gaaaaacctt gtaaaaacag
      181 ttttttggca tatttatttt ttataaatat ttgtaaaata actatcaatt ttttagttaa
      241 aagattggaa atgagtgggg gaggaattga agaaaggatt tttgagagat gatatagtag
      301 gttttatttt tagttaaaat aggggagata gaaaaaatga aaaagtgtag aaatattatt
      361 ttatcaatgt tatttattat tactaatata acatcactga ttcctgttca tgtctatgcg
      421 gatgcaggca gagatatcag cagtaatgtc acttcgttga ccgtggaccc aacgaatatt
      481 acagatggag gaaacataaa agtaaaattc tcgtttgacg aaaaaaaaca aaatatccaa
      541 ccaggtgatt atctttggat caattggcca agtgaaggaa atattcgcgg tgaaggattc
      601 caaaaagaaa tcccattaat gattgaaaat aaaaatgttg gaactttaac ggtcagaaaa
      661 gattctgcgc aggttgtgtt taatgaaaat attaaaaacc tagactcagt agaaggttgg
      721 gggcaatttg agatacaggc cagaaacgta accgataccg gtgaagaaaa tacgggacca
      781 ttcaccgtga cgagcggtga taaaacagct actgtaaatg tgacaaagcc agcttctggt
      841 tcttcttcta gcgtttttta ttataaaaca ggagacatgc tacctgaaga tactaagcac
      901 attcgatggt ttttgaatat taacaataac ggcacttatg ttgaacaacc agttaaaatc
      961 agtgatgaaa tccaaagtgg acaaagatta gatccaagta cgtttgaaat taatcaaata
     1021 cacttaggtg aacagaaggt ttatagagga gaagagggaa tccaacaatt tttacaagat
     1081 ttccccagcg ctacatttaa tttttcagta actgataatt acattgaaat aactatacca
     1141 aaaaactttg tcaatcttag gaaaataatg gtgagttata agacgatcat agaaaaccca
     1201 gaacaaataa attttgagaa tcattcggaa gcttggttta aagaatttaa taaacctgct
     1261 gttgatggag aaagtttcaa tcatacagtt aaaaatattt cagcttctgg tggagtcaac
     1321 ggtacagtta gaggcgaatt gaagattttc aaatatatca atgacacaga gattggtatc
     1381 ccaaatgtga ctttcgaatt aagacgagca gatgaacagc ctattcaagg acagtctagt
     1441 atcctgttga cttcgaatga acagggagaa gctagtatca aaggcctgca agtaggagac
     1501 tatgttgtaa aagaaaaaga agcacccaat tggattgatt ttgatccact aagttcaaat
     1561 gaactcaaat tctccataaa cgaaaatgat acagagggag tcagtttacc tatttataac
     1621 aagaaaaagg ttacgaacat cacagcaact aaaatttgga acggtggaac cactccaaga
     1681 ccgagtattt attttaaatt attcagagca agtacaaaca attgggaacc agttcctgat
     1741 gcagaaacaa aacgattaga caatggaata acttcagtga cttgggaaga tatccaacag
     1801 tatgatgata gtggaaacga atatacgttt aaagtgcaag aagtagatca aaacggaaat
     1861 gattatgtcc cttcaggtta tcaaaaaatt gaaaacgggc tagtcgttac aaatgagaat
     1921 aaagaagtca tttcagtctc aggacaaaaa acatgggaag acaatgagaa ccaagacggc
     1981 aaacgaccga cgacgatcac agtaaatcta ttagccgacg gtcaaccgat ccaacacaaa
     2041 gaagtaagtg aaaaagatga ttggcgctat cgtttcacaa atctgccaaa atacagagac
     2101 gggcaagaaa tcatctatac tgtaacggaa gacaacgtgc ccgaatacag tacgacgatt
     2161 gacgggtata acatcaaaaa cagttataca ccggaaaaaa caaatatatc tataccaggt
     2221 agccatataa caccgtggaa aaattttcca gagaagacag agagagagaa aattagaaat
     2281 ctattctctc aaaaattgca aatatttgct catacaggag aatccagcga acaaagaagc
     2341 aattatatta gtaagagagc tgtacctaaa cagataacaa ataaatcaaa aagcattctt
     2401 cctaaaacaa gtagtaaaaa atctagtttt gctgtaatca ttggtttatt gatagtaact
     2461 atctgtgctg ggatattctt acaaaaatat aaaaaagttt agttagaata taatattttt
     2521 tgttaaatat gactgaaatc aaaagaataa aattccgatt tataatcgaa aaaagaagca
     2581 tttttatata tgcttctttt ttcgtagaat caataaatat ggatttggct aactatggaa
     2641 gaatactcgt attagttttc agcgttcata ctagttgtta cattgtttga ttggccaaaa
     2701 gaatttctga tatattttgt gctaaataaa gaaaaagacg cttgaattgg gaaataagaa
     2761 aactactcta tcattacaaa ttttcataaa ctggcagatg ttctaagtaa acaagtatct
     2821 tttcaaagtc ggaaaaatcc ataatcaacg tagtggtatt gatatttgga tgaaagccta
     2881 cggtatcttg acgatcaata gcagcatcga tcacgacttg gatttttttc tctttgtcgt
     2941 gcatcaatgc taatggagtc actgtacctg ctggaacgcc taggaggtca ggaagctgtt
     3001 cttcagaaac aaaagataag cgcttttctt ttaattgttc agctagttgt tttaaatcgg
     3061 cgtgcttttc atcaggcaag atgacaagat agatttgttt tccttttctt gatttcaata
     3121 atagattttt tacttgcgga ccaggtaggg aaaaagggac atttttgact gacgtaatcg
     3181 caggatgatc gactttttga aaagagatac ccagtttttt tagtagttca taagcttctt
     3241 gttcggttgc acgactcatc gcgcatcacc ctcctttttt tattagtgta tcataaaaaa
     3301 gagttgacaa atgaaaaata atcatgtaaa ttacacgtaa agttaaaaca aatgacatcg
     3361 aaaagaagag tagccgttag gtgtttttag agagtctctg gttggtggaa agaggcagca
     3421 gttaaccgtg aaacacatct tggagtcgat tttctgaagc gtatagtagg aaaatccggg
     3481 gagcccgtta cagctgcggt cgttgctaga tgaccagata aggctggctt ttccgtgagg
     3541 agtcggtaaa aaaaggtgga accacgtgat taacgtcctt tgatcttcgg atcaaaggac
     3601 tttttgtttt catctgaacg gcaacgactg tgtgaggttt tttctgcaag gaaaaaataa
     3661 acagaggtgg aaccacgtat aatacgtcct cttggcattt gccaagagga tttttttatt
     3721 ttaaaggagg aaaagaagat ggagtatttg attgaagtta tgccgcaatt attagtagga
     3781 gcactcacaa cattgaaatt atttttattt accttgattg gctcattacc attagggatt
     3841 gttttagcat taggattgtc tttgcatttt aagccgctac gctacttgct tcaaacatat
     3901 gtatgggtta tgcggggaac accgctgttg ttgcaactga tttttatttt ttatgggttg
     3961 ccgacgattg ggatcgtttt tgatcgtttc gaagcagcac tcattgcctt tatcttaaac
     4021 tatgcagctt attttgcaga gatctttcgt ggagggatac aatcgattcc tcaaggtcaa
     4081 tatgaagcag cgcaagtttt gcaattaagt ccctttcaaa cggtgacgaa aatcgtcatc
     4141 ccacaagtga caaagattgt tttaccttct gtagggaatg aagttatcaa cttggtcaaa
     4201 gattcttcct tgatctatat attaggttta ggtgacgtaa tgcgtgctgg gaaaatcgcg
     4261 atggaacgcg acgcgacttt actgcctttg ctaggcgtgg caatcatcta tcttacattg
     4321 actggtattc taacaatcgt aatgaaacaa gttgaaggaa aattaaatta ttaccgctaa
     4381 ctgataggag gaagaaatat gattgaattg aagaaagtga acaaagcatt tggtgaaaaa
     4441 aaaataatca aagatttgaa tctaatcatt ccagatggga aaacattagc tatcgtaggt
     4501 ccttccggtg gcggtaaaac cacgctttta cgtatcttgg ctggcttaga aagttctgac
     4561 agcggagaat tttatttaga cggtgtaccg ttcaacccat tcgagcttaa aatgaaagaa
     4621 caagttgtag gagtcgtatt ccaagacttc cagctatttc cacatctgaa tatttttgaa
     4681 aacattacct tagcaccaaa aatggtattg aaacagacaa aagaagaata caccaaaaaa
     4741 gcagaagaat tagcggcttc attagggatt agtgatttat tgaatatcta tccttatcag
     4801 ctttctggcg gacaaaaaca gcgggtagcg attgccagag cattggctat gaatccacgg
     4861 gtattggctt atgatgaacc aacaagtgct ttagatcctg aattacgcca gcaagtcgag
     4921 cgtttgatcc tatcattgaa agaagatggg gtaacccaaa ttattgtcac ccatgattta
     4981 gtatttgcag aaaatgtcgg tgatcaatta ctaaaagttt ccccaattca ttaagaaagg
     5041 agaagataga gatgaaaaaa accagttttc tattcgcttt gatcgcaggt cttcttcttt
     5101 ttgcaggctg ttcgaacaaa aaaacttcag cagataattg ggcgactatc aacgaagaga
     5161 aaaaaatcgt tataggttta gacgatacat ttgttcctat gggatttaga gataaagacg
     5221 ggaagctcac aggatttgat attgatttag ccaaagcagt ctttgaacaa tatgggatta
     5281 cggtagactt tcagcccatt gactggtcaa tgaaggaatt tgaactgaac aatggaacga
     5341 tcgatttgat atggaacggc tattccaaaa cgccggcaag agaaaagaaa gtgcaattta
     5401 caaaacctta tatggaaaac gatcaagtgc tgatcacacc taaagaatca ggaatcacaa
     5461 actttggaca aatgaataat aagagattag gtgcgcaaaa tgggtcatct ggttatgatg
     5521 tatttacgaa acaaccagaa gtattaaaag agatagtcaa aaaccaagat gctgttttgt
     5581 atgatagttt taatgaagca ttgatcgatc tgaaaagcag acgggtcgat ggtctgctga
     5641 tggataaagt atacgcagac tattatttga aacaaagaaa cgaactagaa gacttcaaca
     5701 taacaaaagg ttcttatgag agtgaggatt ttgcagttgg tgcacgcaag tcagatagta
     5761 cattagtgaa aaaaatcaat gaagccctag atcaactaga gaaaacaggt gaattccaaa
     5821 aaatatccga taaatggttt ggcgaagatg ttacgccgag aaattaacaa ataggggact
     5881 gtgacaaaag acgtgtcaca gtcccttttt ccgaataaac agtgttctcc tgccaactcc
     5941 ttgtgactcg gagctgaatg gcaggctcac aacctcttgg taaacggtgt tcaaaagcca
     6001 gtttttcggt attaagccaa atttatgcaa aaatgtagaa agccattttc acaaaatttt
     6061 tcttactact cgaaactaag cgactttttc acaacctcct ttacataatt ttattttgtt
     6121 gtggaagggg tttcgtgtta ttctaaaaga ggaacgattg aaggttatag gaaaggaaga
     6181 gatgaatgga acacaaagaa cttacctcat ttccaaaaga ttttctgtgg ggctctgctt
     6241 ctgcagcgta tcaagtagaa ggagcttggc aagaagatgg gaaaggagaa tcagtctggg
     6301 atcgatttgt acggatacct ggaaagacat ttaaaggaac gaacggtgat ttggcagtag
     6361 accactatca tcgttataaa gaagatattg ctttaatgaa agaacaaggt ctgaaagctt
     6421 accgcttctc tgtagcttgg acacggattt ttcctaatgg acgaggagaa gtcaatcaag
     6481 ctgggctcgc tttttatgag agattgatcg acgaattgat tgaaaacgat attgaaccta
     6541 tgttgacttt atatcattgg gatcttccac aagcattgca agatgaatat aatggttggg
     6601 aatcacgtca gatcattgaa gatttcactc attatgcaga aacattattt gaggcattcc
     6661 gcgggaaagt ccattactgg attagtttga atgaacaaaa tatcttcact tcattaggtt
     6721 atttgttagc agcacatccg ccaggtgtga cagacccgaa acgaatgtat gaagttaacc
     6781 atattgctaa tttagcaaat gcatctgtca tcaataaatt tcacgagtta gaaatgcctg
     6841 gaaaaatcgg accaagcttt gcgtatacgc caaattatcc aattgacagt aatcctgaaa
     6901 atgttctggc tgctgaaaat gcagaagacc tgatggccaa ttattggatg gatgtctata
     6961 tgtggggaaa atatccgatt gctgccatga ggttcttaga agaaaaagga tgggcaccaa
     7021 cgattgaagc tggcgatgca gaattgctag aatctgctaa accagatttt cttgggatca
     7081 attactatca aacggcgacg aatgcattca atccattaaa cggagttggt gctggaaaaa
     7141 tgaatacgac aggaaaaaaa ggttcatcag aagaaactgg agttcctgga atgtataaaa
     7201 aagttgagaa tccatttgtc gaacgaacaa attgggactg ggagattgac ccagaaggat
     7261 taaggatcgg actgcgtcgt atcacaagcc gttaccgcat gccggtactg attacggaaa
     7321 atggcttagg agagtatgac aagttgacag aagataaaca aatccatgac gactatcgaa
     7381 tcaattactt acaaagccac gtcaaagcca tcaaagaagc aatttcggat ggagcagaag
     7441 tcctaggcta ttgcacctgg tcttatacag atttgcttag ctggctgaac ggttatcaaa
     7501 agcgatatgg atttgtatat gttgatcaag acgaaacaca agaaggatct ttggaacgat
     7561 ataagaaaga cagttattac tggtaccaaa aactgattga agaaaatgga gagaaccttt
     7621 agttcacata cttaaaacaa gaaggtacgg ctggatatgg ccgtgccttt tcttgataaa
     7681 atcatttaga aattaagaaa aactgtaaaa taagctgaaa aatgaaagaa agcgtcagtt
     7741 ctttcaaaca aaactttagt tcccttactt ttttttgtat aatgaaactt gatgaaatga
     7801 gggataataa atggcacaaa agaaacggcc tactaaaaaa aggaaaaaga cgaaaaaaac
     7861 gaaaaagcaa aaacagcagc aggaacaaat gctgacgatt ggtgttgggt ttgccttgat
     7921 tttatttgct gtcttcggct ttttaaaatt aggtttttta ggaatattga ttgcaaacgg
     7981 ctttcggatc attgcaggaa atacttacca aatcctctgt cttttattag ccgtattagg
     8041 attttggatt gtcattaaga atacagagtt ttcaattggt aaaaaccgac gctggtttgg
     8101 cggcatactc ttttatttcg gtatcttatt attgttacat gcccatttat ttggcaaact
     8161 ccatactggg gaaccaaata tcatggggac gacatgggat ttgttagcca gtgatataaa
     8221 acaaagtcaa gtagacaata acgttggtgg tgggatgatt ggagcaatcc tttatcattt
     8281 tacttacttc ttgattgcac agcctggaag ttatttagca gcagtgctat tactggcagg
     8341 aggagcattc ctgttcagta atctggaagg ctatcagctg ttaaatggta tccaatcaat
     8401 tggagaacga gtacaggaat tattggaagg tgatcctgcc aagcaagctc gaaagcaagc
     8461 ggcaaaggaa gagagaatga aacagcgcgc agaagcgaaa gaagcaaggc gcttagctgc
     8521 aaaagaagct gcagaaaaag aagctgcaga atatgagaag aaaaaagcgg tgagccagca
     8581 aagggaaaat actgctgatg aatggcagga accagaaaat acagagccgg agcagctaag
     8641 ttttgttcca atcgacagtt ttcaagaaaa tatccatcct gcgaatctag agaaaccagt
     8701 accagataca ccaaaacaga ccaatacagc agaaggtttt tcagatgagt tgccagagga
     8761 tgacggtacc tcattagaat ttgagattga agcagaacag gaaaatcaag actatgaatt
     8821 accgacagtt gatttgcttg attctatacc aactgtggat caaagtgatg agtataaaaa
     8881 aattgaaaaa aatatcggcg tattagaaca aactttccaa agtttcggtg tcgatgccaa
     8941 ggtcgtcaaa gccagcttag gaccatccgt cacaaaattc gaagtccagc cggctgtagg
     9001 ggtaaaagta agtaaaattg tgaacttaac agatgatatc gcgcttgctt tagcggcgaa
     9061 agatgtccgg atggaagcac cgattccagg taaatcactg attggtattg aagtaccaaa
     9121 tggaaagatc agtatggttt cctttagaga aatcatcgaa gcacaaccaa accacccaga
     9181 taaattacta gaagtacctt taggacgtga tgtttctgga cgggtacaaa cggctgatct
     9241 ttcgaagatg cctcacttac tcgtggctgg ttcgactggt agtgggaagt cagtagcaat
     9301 taatgggatt atcacaagta tattaatgcg tgctaagccc catgaagtaa aactgatgat
     9361 gatcgatccg aaaatggttg aattgaatat gtacaacggc atacctcatc tgttgacgcc
     9421 ggtcgtgacg aaccctagaa aagcagcgca agccttacaa aaagtagtac aagaaatgga
     9481 agaacgatat gaaaaattcg cggcaaccgg agtaagaaat atttctggtt acaatgaatt
     9541 tgttcaacag aaaaacttgg aaaacggaac gaaacatcca accttaccat tcatcgtagt
     9601 gatcgtagat gaattagctg atttgatgat ggtagccagc aatgaagttg aagatgcgat
     9661 tattcgttta gcgcaaatgg ctcgggcagc ggggattcat atgatcctag ctactcaacg
     9721 tccaagtgtg gatgttatca caggaatcat caaagcaaat gtaccttcca gaatggcatt
     9781 tgcggtatca agcggtacag attctcggac gatcattgat tctaatgggg cagaaaagct
     9841 cttagggcga ggagatatgc tattcttgcc aatgggtgag aataaaccga tccgtgtgca
     9901 aggagcattc atttctgacc acgaagtaga acgggtcgtt caatttgtta cagatcagca
     9961 agaagctcat tatgaagaga aaatgatgcc gacagatgaa gttgaaacga caggtgctcc
    10021 agaacagcca caagatgagc tgtttgaaga agcaaaagca cttgtcgttg aaatgcaaac
    10081 ggcaagtatt tcccttctcc aaagaagatt tcggatcggt tacaaccgag cagcacgttt
    10141 agtagatgaa ctggaagctc atggagttgt tggcccgtca gaaggaagca agcctcggaa
    10201 agtattcatt gagcaggaag aaacatcaga aacgttgccg gacgataatg acatgtaaaa
    10261 cttgtgtgat gaagggttga aaaacgaata accaataaaa agacaaggca tgatgatacc
    10321 aaaagaaaaa atggtttttt tccatctttt ctaatacggt atgatgtctt gtctttttag
    10381 tttttgtgag attaaagttc tattcgattt atttgtgaag tattgcatta aggaaatgaa
    10441 tcatgtatag tatgtagcga aactttgaga aaaagaggga aaatactttg gaaaaacagt
    10501 ccatttatgg cttgacgaat gaagaattaa tcaattggtt tatagaaaac ggcgagaaaa
    10561 aattccgagc tgcccaagtt tgggaatggt tgtatcaaaa acgtgtaagt aatttcacag
    10621 aaatgacgaa tctatcgaaa caattgatcg aaaaattatc ggagcatttt atcatcaatc
    10681 ctctaaaaca gatggtcgtc caagaagctt cggatggtac agtaaaatat ttgtttgaac
    10741 tccctgataa aaacatgatt gagactgtac tgatgagaca agaatatggc ttgtcagttt
    10801 gtgtgacgac acaagtagga tgtaatatcg gctgtacgtt ttgtgcaagc gggctgctca
    10861 aaaagaaccg tgatctgact gctggagaaa ttgttgcaca gatcatgatg gtccaacatt
    10921 attttgatga gcgcaatctt ggcgaacgcg tttcacatgt cgttgtaatg gggatcggtg
    10981 agccatttga taattacgac aatgtaatgg actttttgca tatcatcaat gatgccaaag
    11041 gattggcaat tggtgctcgg cacattacag tttccactag tggattagca cacaaaatca
    11101 aggaattcgc taataacggc ttgcaagtca atctggctat ttccttgcac gcgccgaata
    11161 atgaggtgcg aacatcgatc atgcggatca accgaagttt cccaattgag aaattgatgg
    11221 aagctgtgga tgagtattta gagaaaacaa accgtcgaat cacgtttgaa tatatcatgt
    11281 tgaatcaagt aaatgataga ccagagcatg cgcaacaatt agcagatcta ttaaaagata
    11341 agaaaaaatt agcatatgtc aacttgattc catataatcc agtcagtgaa catgatcaat
    11401 attctcgcag tccaaaagct gatgtattga gattttatga tgtattgaag aaaaacggcg
    11461 taaactgtgt gatacgtaaa gaacatggta cagatattga tgcagcttgc ggacaattgc
    11521 gaagcaagca aatgaaaaaa acagttgcca aataatcgta tcctactcgt tttttcaatt
    11581 accataaaag gagcagtttt cttaattaag gaactgttca acttaaataa gagaaaagat
    11641 tctatagcga aagctataaa gctttttttg acaagaaaaa ccctcatcaa gcaaagaaat
    11701 gtcctcaatt tgatgacagc ctgcttgatg ggggttttag caattattta actgattttc
    11761 ttatatcctt gctggctttc ttgtgtagca tgataagttt ctacgtcaag aatggcctgt
    11821 ttaaattttt ctcgggtgat ttcataaggc acatgtttaa tatcatttgt tgccatggct
    11881 ttatctaaga ttgcttctaa atcggtcgtg acattgagat tcatgtcggt taacgatact
    11941 ggaagttggt tctttttcat aaacacatag attttatcaa acaaatcgaa ctgctgatcc
    12001 atcatcaata aggtcagtac accataactg acagatgctc cgtgaaggta tccctccatt
    12061 ttttctagaa cagtcgtacc gtaatagaat gagtgtgcca tagcagaatt atagttattg
    12121 ttaactaaaa cagaaaccaa tccggtagta acaatgatat ccgaaacgac ttgttcaatt
    12181 gcatatgagg gacgatcgtc ggcactatct ttcaatgctt gcgtaccata agtcaagaga
    12241 cgatcaatgc acatttttcc gatttggatt cctaatctat ccgtgtagcc tagatcatcg
    12301 ccacgagcag aaaaagtaga ttcgacttct ttggataaag catcgccaat acctgcccat
    12361 aagtatttat caggtgcttc ggcaataatt gctgtatcaa tgaaggtgtg tacaggtggt
    12421 tttgaaggaa agaagagtcc gtccattacg ccttcttcgt tatagatgac acataccgca
    12481 gtgacgggcg aacagttcga accaatagtg ggaaaggtga agactggttt agctagtttt
    12541 tcaccaatga cttttacagt atcgcaagca cggccaccac cgactgcaaa caggacgtct
    12601 gctttttgga cactttcatg ctggatcaac tgttcaatat ttccataagt tgaattaccc
    12661 ccgtaccaga gactggcgac aacctctatt tcctgtgtca aagctacttt tattttgttt
    12721 tctgcctttg aaagtgcagt cttccctcca atcagcacta cttttgtccc atattctttg
    12781 acgattccat ttgctttttc atacgcatct gttccaatgg tataatttgg taagaaaaca
    12841 ctttgactca tattcatttc cttctttctt cgttgaaacg ggttaaaatt ttaaagagtg
    12901 atcgatctga ctgcggctgt agatggagta gaggtgaacc taagacagga taagtgatga
    12961 ataaggtggc atgaacacct tgaaatcatc aataagtatc ctaaaaacga acaagtacaa
    13021 aaacattgaa aacgacaata atgaattttt gttcctctta gtatttctac cgattccctt
    13081 tgcttttttg taattatcta aaaattctaa aaattaagat tattatatga aaacaggttt
    13141 tatttgtcaa ttcataattg gtttttttta aagcaaaaga taatctatat catgatgaac
    13201 aaacagagtt ttttcctcca attgatggct ttcctgatct ttcctatttt gaataaagtg
    13261 tttctgtata atttttttat tttataagta agataaataa tgaaagaata gagatactga
    13321 ttggtagcta aagatttttg agcgagaaca tttcgttttt tcaaaaggac tgttagaata
    13381 agcaagaaca agggggaaga cgtatgataa tcagtgaatt tgaccgtaat aatccagtat
    13441 tgaaagatca gctttctgat ttactgagac tgacttggcc ggaagaatat ggagacagct
    13501 cggcagaaga agtagaagaa atgatgaatc cagaacgaat cgcggtagca gcggtagacc
    13561 aagatgagtt agtaggattt attggtgcaa tccctcaata cggtatcaca ggttgggaat
    13621 tgcatccatt agttgtagaa agctcccgac gaaagaacca aataggtact cgattagtca
    13681 attacttaga aaaagaagta gcttccagag gaggaatcac gatttattta ggtacggatg
    13741 atttagacca tggaacaacg ttaagtcaaa cggacctgta tgagcataca tttgataaag
    13801 tggcttccat ccagaacctt cgtgaacatc cgtatgaatt ctatgaaaaa ttaggttata
    13861 aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa accggatatt tggatggcaa
    13921 aaacgattat tcctcgacca aattctcaat aataaaaaga ggttgtgaca gaagtaatca
    13981 gcttcaagaa ataagaagga atttacgaaa attacttctt gaatttttgt gaaattccaa
    14041 cttatttccg aagaagctac ttttggaaca ccgtgtacca gaggttgtga aaaagtcgct
    14101 tagtttcgag tagtaagaaa aattttgtga aaatggcttt ctacattttt gcataaattt
    14161 ggcttaatac cgagaaactg gcttttgaac accgtttatt cagataaggg actgtgacaa
    14221 gacttttgtc acagtttctt tttgtttatt cttttttatc tttgtatggc aataatagac
    14281 aatataacga aaattattta atggcttttg gaagaccatg gaaaaccttg tacaaagcta
    14341 ttttttctga aaaattcctt gaaacctcac gaaaaaatca ttattttatg aaaaagacaa
    14401 aaaaaagtgt tgatttttta ataattattc attaaactct taaacaactt cagaaaatat
    14461 taattattct gaaaattcca ataaaggggt acgtaaaatg aaaaagaaat ttacatttgg
    14521 tttggttgcg gtatgcggcc ttgtacttgc tggatgttat ggcggcagtt cagccacaag
    14581 cagtagttca gcaaaaagcg gttcagcaga tggaggaggc gtatttaact tagtcgtgcc
    14641 tcaagaaatg cctacagctg acttatcagt tgcaacagat acaatcagtt ttacagcatt
    14701 gaataacgtt tatgaaggaa tttaccgtct tgatgaagac agcaaaccac agccggcagg
    14761 agctagcgag ttggcagaag tcagtgaaga cggattaact tacaaaatca aattacgaga
    14821 agacgcaaaa tggtcaaatg gtgatcctgt tacagcagcg gattacgttt atggctggca
    14881 acgaacagta gacccagcta cagcttctga atatgcttac ctgtatgcgc cagttgaaaa
    14941 tgcagaagac attacagctg gaaagaaaga taaatcagaa ctagggatca aagcagttgg
    15001 tgactatgaa ttagaaatca aattgacgaa acaaacacca tatttccaat atctgcttgc
    15061 tttcccatca tttttcccac aaaaacaatc cgtagtggaa gaaaatgggg atgcctatgc
    15121 atctgcaagt gataaagctg tttataatgg accattcaca ttagaagatt ttgatggacc
    15181 aggaacagat acagaatgga cttataagaa aaatgatcat tactgggaca aagatacagt
    15241 aaaactttct gaaatcaaag tcagtgtagt aaaagaatct tctactgcat tgaacttatt
    15301 caaagatggc caagcagatg acgtgatctt gtctggtgag ttagcacagc aaaacgcaaa
    15361 cgatccagct tatacttctg ttaaagaagc gcggacaagc tatatcgaat tcaatcaacg
    15421 tgaaaagaat tcaccattca acaatgtgaa cctaagaaaa gctatttctt attctatcga
    15481 tcgtgaagct ttagtaaaac aagtactagg tgacggatca gttgtctcta caggattgat
    15541 cccatcagat atggtaaaaa gtccaacaac gaacgaagac tttgcaaaag aagctggaaa
    15601 attagtttct tatgacaaag ataaagcaaa agaatattgg gaaaaagcga aaaaagaatt
    15661 gggcgttgat tctttagaat ttgatttgat ggcttctgat gatgattcat cgaaaaaagt
    15721 aatcgaatat gttcaaaact caatccaaga aaacttggat ggcgtaacag tcaaaccaac
    15781 acctgttcca ttctctgtac gtcttgaccg ctcaacttcg ggtgattttg acgcagtact
    15841 aggcggatgg gctgctgact atgcagaccc aagcagcttt actgatttgt ttgtcacagg
    15901 taattcatac aaccgtggtc gctggagcaa tgcagaatat gacgaagcag ttaaagcagc
    15961 ttcatcaaca gacgctggcg atgaacaagc ccgttgggat gatttccaaa aagcaatcaa
    16021 gatcatctct gatgatatgg gtgtcgttcc ggtgtaccaa aaagcagaag gtcacctagt
    16081 gaatgaaaaa atcaaaggta tcgttcacca tgcggcaggc gcttcatggg attacaaatg
    16141 gacatacgtt gaagaataat cgttaatacc gtgagcatga gaaaaaacga ggctgcggca
    16201 aatgtcgcaa cctcgttctc atattcactt atcctaacaa agaaaaaagc atgaaaatag
    16261 cagaaagagc aattagtatt ccggcaatga tcagccaaaa gctgtacata ccgtagctgg
    16321 cagacgagag cgaggaaaat tctggttttt tctttcggtt ccttgttcgt gcaagatgga
    16381 ccgatcgctg aaaatgatcg aagaaacccg aagaaaagac ccataaaaaa ccgccgataa
    16441 tcagaaacgg caatgaccat aagaatagat cattagataa ttgaatgagt gtaaaggaat
    16501 ggttgatgag attttttatt aaaataacca gaatcagtag aaaagctact gcgtagggga
    16561 tagatttttt tttcatttga ttgctccttt atttttggtt cattatctaa taatgtagcg
    16621 aaacatctgt cagaagtcaa aagctagggg gaaacattag tgaatagtta tatcaaatat
    16681 gtattaaaac gtgtcttttt catgatcatc acgctttggt taattgcaac aatcaccttt
    16741 ttcttgatgc aattgctgcc gggcacgcca tatacgaatc aggaacgttt gagtcctgaa
    16801 acaatcgaga tgctaaataa acaagtcggt ttggataaac cagttatcgt acaatatggg
    16861 atttatcttt ctaatttgct tcaaggagat tttggaattt ccttccaatt taaaaaccag
    16921 ccagtcgcac atttgttagc tggaagaatc ggaccatcct tgcagttagg gttacaggca
    16981 attatctttg gaacagtcct agggacgatt ttaggaacga tctctgccat gaagcaaaat
    17041 acatgggcag atacatcttc tacactcgta gcgatattag gtcgttccat tccaaacttt
    17101 gtatttgctg ttttattaca atatatcttt gcaatcaaat tacaagtttt gccaatcgca
    17161 aaatgggatg gctttgtgta cacaatctta ccaacaattg cgttggccat gtcgccgctt
    17221 gctgactctg ctcggtttat ccgtacggaa atggtggaag tactgcatag tgattatgtg
    17281 gaattagcaa aagcaaaagg attgagccgt tgggaaatcg cctttaaaca tggtcttcga
    17341 aatagtttga ttccattgat gactttatta ggaccgttag ctgtagcatt gatgaccgga
    17401 tcactggtgg tagaaaatat ctttgctatt cctggtatcg gggaacaatt cgttaagtca
    17461 atcacaacaa atgattatcc tacgatcatg gctgtaacga tcctttactc atttatgctg
    17521 atttttgtta ttttgatcgt ggatctattg tacggtctag ttgatccaag aattcgtgtt
    17581 tcagaaggga gccgaggata aatggaaatg ataccaaagc gttatgaaac agttgcagat
    17641 atccctgcgg atgaatttct tccattacaa aaaaataccg aagaagaacg tgaacagatt
    17701 actgcacctt ctttgaattt tatccaagat tcatggcgcc gtttaaagaa aaacaaagct
    17761 gccgttattt caatggtttt actattggtc atcattttga tctctgtgat cacaatcttt
    17821 gtttctcctc atgatccaac tgcccaaaac gtggcttata tcaatttgcc tcctcgtatt
    17881 ccgggaatca atatcgatgg attgaatgga aaagcaatgg ttggtggtga acttgttgat
    17941 aaatatgctc aagcaaacgt cccttcaaac gtaaatttct ttttaggaac agacggtttg
    18001 ggacgtgatg tattaagccg cttatttatg ggaatgagaa tctcgttgct gatcgcattt
    18061 attgctgcat tactagatgt aacgattggt gtagcttacg ggttggtttc tggtttgtta
    18121 ggcggacgtg tcgataacgt gatgcaacgt tttttggaaa tcttatcagg aattccaaac
    18181 ctcgttgtca tgattttgat gttggtcgtt tttgaaccag gaattttttc aatcgtggct
    18241 gctatggcca tcacaaactg gataccaatg gcacgtatcg ttcgtgcaca aacaatgaaa
    18301 ttaaaagatc aagaatatgt tttagctgga ttgacattag gtgaatcaag aatgaaaatt
    18361 gcattcaaac atattttgcc gaatatctct agtgtcatta ttattcaaat gatgtttagt
    18421 attccatctg ctattttctt cgaagccttt ttaagcttta tcggtttagg tcttacgcct
    18481 ccgtcagctt cattaggaac gatgttgagc gaagggtaca agacattcct gtatctgcct
    18541 tatctattat ggattccggc agtaacttta tcggttatca tgattggctt taacttactt
    18601 gctgatggat tgcgtgatgc ctttgatcct aaaatgaaag agtgaagacg atgacgaaaa
    18661 ttttagaagt gaaagactta gaaatctctt ttgatacgta tgctggaaag gtacgagcga
    18721 ttcgcggtgt caattttcat ttgaataaag gagaaacatt ggcgatcgtt ggggaatctg
    18781 gaagtggaaa atcagtgacg accagaagca tcatgcgctt gctgtcaagc aacgccaata
    18841 tagatgctgg tcaaatcctc tttaaaggta acgatatcgt tggaaaatca gaaagagaga
    18901 tgcaaaaaat ccgtggtaaa gaaatagcga tgattttcca agatccaatg acctctttag
    18961 atccgacgat gcccattggg aagcaagtcg ctgagtccct tagaaagcat aataaagttt
    19021 caaaaaaaga gggacaaaaa gctgcattag aattgcttaa attagttgga atacccgaag
    19081 ctgagaaacg tatcaacagt tatccgcatc agttctcagg aggtcagcgt caacggatcg
    19141 tgatcgcgat tgcactggta tgttatccgg aaatcctgat tgctgatgaa cctacaacag
    19201 cgcttgatgt gactattcaa gcacagattt tggagctgct gaaagaaatt cagacaaaaa
    19261 tcgatacatc aattatcttt atcactcatg accttggtgt agtagcgaat gtcgctgatc
    19321 gtgtcgctgt catgtatgga ggaagaatcg ttgaagttgg aacatcggaa gaaattttct
    19381 acaacccgca acatccctat acttggggac tgcttggttc aatgccgaca ttagatagtg
    19441 cgaacgatcg attgtatgct attcctggtt ctccaccgga tttattgaac ccgccaaaag
    19501 gagacgcatt ctatccaaga aatgaattcg cgatgaaaat cgatgcagaa caagaaccac
    19561 cattttttga actatcaaaa acacacaagg cagcaacttg gctacttgct ccgcaggcac
    19621 ccaaagtgac accgccagaa gaaatccaac gtcgctgggc tatctttaaa gagaaacaaa
    19681 aacatactta cggtcaagga ggaattgctg atgccaaata aactacttga agttaaagga
    19741 ttgaaacagt atttcaatgt aggaaaaaag aatgaagtac atgctgtaaa tgacatcagc
    19801 tttcacattt atgaaggaga aacattcggt ttagttggag aatctggaag cggaaaatcc
    19861 actactggcc gtacgatcat tcgcttgaat gaacctactg atggtgagat attattcgac
    19921 ggtcaagata taacgaagat caaagataaa aaaggtttga cgaaattccg gcatgatgtt
    19981 cagatgatct ttcaagatcc atacgcttcg ttgaatcctc gtatgaaagt cagagatatc
    20041 attgccgaag gaattgatgt taatggacta gctaaatcat ctgaagagcg tgcaaaaaaa
    20101 gtcgatgatc tattgagaac tgttggattg aatccaagcc acggtactcg ttatcctcat
    20161 gaattttcag gtgggcaacg ccagcgtatt ggtattgcac gagcattggc agtaaaaccg
    20221 cgttttatca tttgtgatga accaatttct gcactagatg tgtcgatcca agcccaagtg
    20281 gtcaatttgt tgcaagatct ccaaaaagaa catcaattga cctatctatt tattgcacat
    20341 gatttgtcga tggttaaaca tattagtgac cgaatcggtg taatgcataa cggcttgtta
    20401 ttagagatgg gaacaagtga tgaaatctat aatcacggtg ttcatcctta tacagaaagt
    20461 ttgttatctg ctattcctct accagatcca gaccatgaga gaaaacgcag aagaatcaaa
    20521 tatcaacctg aaccagatga cggacaagta cgtcaacttc gtgaaatcgc accagaacat
    20581 tttgtctatg caactgagca ggaagtccca tattacgcaa aaaaactaaa acgacaaaag
    20641 gaatctcttc tggttgctaa ctaggaggga catagatgct aaaagctttt aaattccgta
    20701 tgtatccgac agaagaacaa aaacagcaat tgattcgtac ttttggatgt gctcgattta
    20761 cctataacca tttgctgaaa aagcggcaga aaagttggca acaaacagga gtagccaatt
    20821 tttcgctcac acccgctaca ttaaaaaaag aatacccatt cttaaaagaa gtagacagtc
    20881 ttgctttagc aaatgcacag cttaatttgg atcgggcttt tcgcaattat ttcaagggac
    20941 gtgccagctt cccaaagctt aaaacgaaaa aaagtatgtg gcaatcttat acgaccaaca
    21001 accaaacccg tacgatctat ctggaaaatg gctacttaaa gttaccaaaa caaaaagaac
    21061 taatcaagat aaatcagcat cggccagttg aaggatcgat tcgctccgct acgatttctg
    21121 ctcgatataa tgaagaattt tatgtcgcac tactttgtga tgtttcgcca gtaaaaaaag
    21181 aatctttagc taaatggatc gggatcgcct atcatccgaa gacattgatc caaacatctc
    21241 gacctctaga agtaacttta ccaaaatttc accagacaga agaaaagctg caacatgcac
    21301 agcggaaatt gaatgtgaaa gtccgctccg ctcaccatag aaagatcagg ttagatcagg
    21361 caagcaatta tcagaaacaa aagagaaaag taatggatct ttatctgaaa caaaagaacc
    21421 agcgagaaga ttatcttgaa caattatctg ggaaattggt caaacaatat gactacttat
    21481 ttgtagaatc ttttccaaaa gaagaggcac atgctgattt ttctattcac gactggcata
    21541 aattaataac aaaattgcgt tataagtccc aatggtataa taaaaaattt cttttgatca
    21601 ataccgacgg agcagaagaa agcaattccg taagaaagag ccaagtagta gaaaaaatgg
    21661 gcagacattc ggttatcaag ggataaaaaa tgaatgaaac tgataggagc gttagggtta
    21721 gccttgttaa taaaaagtct taatgacttt tgtttctagg aatttttctt ttgaagaaaa
    21781 gctcactaat tatgacaaac caataaaaca atagcagaaa aagttcgtac gagctttttc
    21841 tgctattgtt aattttttat tcagaaagtt tacgaataca tcaaaattct gaaacaaaaa
    21901 acgtaactca caaagagttg tttttgcgtt ttttcttgaa tgattaggtc tgaatctttt
    21961 gctcacaacc ttattcatgg tgccccggct tgagctgata gatttttgaa ggacggccga
    22021 ttcctacagg acgttcaccg atctcatcga aatattggag catagctttc ttaaaattgg
    22081 aatgatcgat cgttttataa tcgatcccta agaatttagc aaatactttt cgagcttccg
    22141 taatcgtgaa atctttacct aaaacttgta gaacttgcgg ctcgtgttcc attttattga
    22201 ctacacggtt gaatgctttc aagatgattt gactatggtc aaaagccaaa gtatctttgc
    22261 cagaagactc tccagtgact aaatcaagcg agatagccac ttctccgcta gataaatgga
    22321 tcttgttact atgacgttct agtgtgaacc agcgtacttc cttggcatca tctccggcga
    22381 ttagcggttc ttctccaata aaggctaagt agctgactgt gacgacccag cctcgcggat
    22441 ctcgattagg cgtactgaat gtatgcagtt gctcaatatt ttcttttgaa ataaccacac
    22501 cagtttcttc ctttgtttct cttaagacgc tttctccagt tgactcatcc ttttggacaa
    22561 agccaccagg caatgcccat gaatttcgat aaggatgccc ttttctttgg atcaacaaaa
    22621 ctttcagctg atcttcttct ttgttatagc acatcagtac gatatcgaca gtcaaagagg
    22681 gcttgtcata ttctggacgt tcctgttttt gataccagtt aagaaattct tcttcacttg
    22741 ctttatgttc ataatataat ttttcttctt ctttagaagc aaaacggttc atctgaccac
    22801 ctccattgac agtaaaagta ctttttacct tattgtagca gactctgaaa aaaaaccaag
    22861 gatttacttg aaaaatctag gttatcattt aaatatgaaa aataaatgga agtatgagat
    22921 tgaataaaaa gaaaagcaga taaaacggca atactttata ccgctcttca cgaatggcaa
    22981 agaagccaca aataacgata tatacgtcaa tgataaaacg caaaatccaa gctgctgtca
    23041 taaaactgtt caaatgccca tggaggttaa aataaccata catacgaata taaaaatagc
    23101 tgaatattaa atgtgctaat gaaagaaaag caatactaag tgtaaaaaaa agatgtttct
    23161 tctgcatgat tgttccagtc ccttactaag atagatagtc tttattatac cacgtgtgag
    23221 gggagaaata aaaagccgtt ttttagtaga tatgaaacaa aacaaaaacc cgcatttatt
    23281 caacctattc atccttttaa aggatagatt gaacgtagat agaagtcgtt tatccagtct
    23341 ttttcatttt gaaaaaaagc taacagcatg ctttttttat ggtatactag caaggaaacg
    23401 aattcgcatc atgcgataaa ggaggaagag ccccgtggca gaaaaggaaa ctttttatat
    23461 cactacgcca atctattatc caagcggcaa acttcatatc ggcaactcgt atactacgat
    23521 tgcatgtgac gcaattgctc gttataaacg gttgatgggc tttgatgtgt tttatctcac
    23581 tggagtggat gaacatggtc aaaaaatcga aacaaaagca gaagaactag gtgtaaaacc
    23641 gcaagaatat gtagataaaa tggcggctga tgtcaagaaa ttatggaaaa cgcttgatat
    23701 cagttatgat aaattcattc gcacgacaga tgattaccat aaagcagctg ttcaaaaaat
    23761 atttgatcgt ttattagaac agggagatat ttacttaggt gaatatgaag gctggtactc
    23821 tgtttctgac gaagaattct ttacagaaac acaacttgca gaagtgtaca aagatgacga
    23881 aggtaacgtg atcggtggta aagcgccaag tggacacgaa gtagaattgg taaaagaaga
    23941 atcatacttc ttccgcatga gcaaatacgc agaccgtcta ttggaatact atgaagaaca
    24001 tcccgaattc atccagccag aatctcgtaa aaacgaaatg atcaacaact ttatcaaacc
    24061 aggattagag gatcttgcag tttcacgaac aacttttact tggggagtac cagtcaagag
    24121 tgatccgaaa catgtggtgt atgtatggat cgatgcatta tccaattata ttactgcact
    24181 aggatatggc tccgaagatg aaagtctatt cgaaaaatac tggcctgcag atgttcagat
    24241 ggtcggtaaa gaaatcgttc gctttcatac gatctattgg ccaatcatgc tgatggccct
    24301 tgatttgcct ctacctaaaa aagtatttgg gcatggctgg ctattgatga aagacggaaa
    24361 aatgtctaaa tcaaaaggaa atgtcgttta tcctgaaatg cttgtggaac gttatggttt
    24421 agatgcactc cgttattatt tgcttcgtgc tgttccgttt gggtcagatg gtgtctttac
    24481 gccagaagat tttgtttccc gtgtcaatta tgatctggca aatgatctag ggaacttatt
    24541 gaatcgaacg attgctatga tcaacaaata ttgtgatgga ctcgttcctg actatgcctc
    24601 acttgtcact ccatttgata gtgaactgtc tacgacggct gctaacgttg tcggacgtta
    24661 ccatgatgca atggaaaaaa tggaattcaa tacagcattg gctgaaatct gggtgctgat
    24721 ttctcgtgca aacaaataca ttgatgaaac agaaccatgg gtattagcaa aagacgaaga
    24781 aaagaaagct gaattgaata gtgtcatgat ccatttagct gaatctttgc gtatcgcagc
    24841 gatattactt cagccaatca tgacagaaac gccagagaag atcttcaatc aactaggatt
    24901 ggactctgaa acaatgaatc ttgaaggatt acactttggc gaatttcctt caggaacaaa
    24961 agtagttgca aaaggtacac caatcttccc acgtttagat atggaagaag aagttgcttt
    25021 tattcaagaa aaaatgtctg aaggtactca aacaaacgaa gatacagtca aatgggatcc
    25081 agaagaaaca gaacttgttt caacgaaaga aaaacaaatc aaatttgatg tatttgaaaa
    25141 agtagaacta aaagtagcag aagtcatcaa ctgtcaaaaa gtagaagggg cagataagtt
    25201 gctgcaattc cgactagatg ctggagacag ccaagaccgt caaatcttgt caggaatcgc
    25261 tgaattttat cctgatccaa gcgaattgat cgggaaaaaa gtcgtcattg tagcaaatct
    25321 gaaaccacga aagatgcgtg gacaaatcag tcaaggaatg atcctttctg cagaagcacc
    25381 agatggctct ttacaagtaa tcgaagcacc aaaatccatg ccaaatggct cagaaatcgc
    25441 atagaaattc aaagggggta tgacactagt tgtgtcatac cccctttttc cgaaatttga
    25501 gaagcaattt tcgtaaattc cttcttatta cttgaagcca atcatataat acagaaacag
    25561 agaactatcc aaaataaatc ctagaaaaag aagtacgagc ttcgctgatt tttgttaatt
    25621 tagaataata ctaataggaa tcgtaaaaac agaaaaaaag cataatgata aagggtcggg
    25681 cagattttaa ttgattcatc tggtactctt ttttattgtc cttacattca taaagtttga
    25741 atgcaatgac tttattacat ctatatttat atctaacagt agcgttgatg tgttaattat
    25801 aagttctatc actttatgtt caattagttt gtgctcttat atgtgtgatt gaaaatatag
    25861 ataaaaaaac aatcatcggc tttgacttcg atgattgttt tctttatttt gtaaaaatcc
    25921 tatcacttga ttttatggta accttttatt tttgtcttca aacggcttgg taagagagca
    25981 gtaagcagtg caaatgggat cgaaaagaca taccagaaaa tcgtacgtcc gataccataa
    26041 tgttgataga gctttgtcaa catcacgtgt aacaagatca accctaaaag caaggagcca
    26101 gctataaacc aatgattatc ccaatgcatg acttcactaa gtgccatcac tccaaaagga
    26161 tagaaataaa aatagatccc gactaaggtc atccgccagt tctttgtaaa tgttccgata
    26221 ttcaataaaa agaaaatcgc tgaaagcaaa ataccgatag gcggaaaaag cccaaccaaa
    26281 aaggaataca acatccccca caagatcatc acaggacctt tgatcaatgc aacgattgcg
    26341 atgaacaaag tcagcaatat tagtttttgt gataacgttg caacagtcat taatccaaat
    26401 aaaattaatg tgaaccaatg ccaatgggac tgctgttgtt ctttacggga aaaatcgacc
    26461 cggttggata aataatctgc taatttcgga taagaacgct taaaatccat aaactgttcc
    26521 tccttcgtgg aaaaggtagg tttagtatac gaaaaataaa aaagaaatac atccgtctta
    26581 agagggagtt tttcactgaa agtggagaaa aaagaatggt aaattaaaat attctcagtt
    26641 atactgaaaa ttataccaaa tttcaaagaa gaaagaggat gggaatgaaa ggcaaactaa
    26701 aacgagaaat caatctgttt ggcgcattag ctaccgttat gggaaccgtg atcggtgcag
    26761 gcgtcttctt caaaactgct gctgttactg ctagtactca gtctacaagt ttgacattgc
    26821 tagcttggct tttgggtggt ttcttaacga tctgtgctgg attgacagtg gctgaattag
    26881 ctacagcaat cccggaaact ggtggagctg taaaatatat tgaagctgcg tatggaaaat
    26941 tgcccagctt tttattagga tgggcgcaaa gcttgattta ctttcctgct aatattgctg
    27001 cattatcgat tatctttgcg acacaattga cgaacttatt acaattatct accgattatc
    27061 ttttgttgat tgcagtgatc accgctgttt ctgtgacagg gttgaatttg ttagggacaa
    27121 aagtcggtgc ttctgtacaa tcagtgacgt tgattgttaa attaatacca attgctgtga
    27181 ttgtcatatg gggattattg accccaggac aaggaactgt ccagttattt ccaatcgaag
    27241 ctggaaaaga tgttactttt gtcgaaggat tgagcagtgc gttgttagca acgttgtttg
    27301 cttatgacgg ctggttaggt gtcggtgcaa tggcagggga aatgaaacgg ccggaaaaag
    27361 atttgcctaa agcaattatt cttggtttga gttttgtaac agtcgtttat ctattgatca
    27421 attttgtttt tttgaaaact ttgccgattg atcatcttgc cggtaatttg aatgcagctt
    27481 ctgaagcatc agacgtcatt tttggaggaa tcggtggaaa gctggtcact attgggatct
    27541 tgatctcggt atacggtgca ttgaacggtt atacactaac tgggatccgc gtgccttatg
    27601 cgatggcttt agaagacgag ctacctttca gtaaacagct gacaaagctt tcaaagaagt
    27661 ttacggttcc ttatgttcct gcggttttcc agttagcagt agcttgtatt atgatgagcc
    27721 ttggttcttt cgacttttta acagacatgt tgatttttgt gatgtggcta ttcagtttac
    27781 tgatttttat cggtgtcttt attttaagaa aaaaagcgcc ggaattgcca cgtccatata
    27841 aagtaccact ttatccaatc gttccgatta ttgcgattct cggtgctatt ttcattttag
    27901 gtatgacgat gatgacgcaa acgaaattag cattgatcgg tataggtgtt accttgatag
    27961 gtatcccagt ctattatcaa aaaaagaaaa aacgctcaga agagtaaaat atttcaacaa
    28021 cagacagaaa aagtacgaac taggtcctct agatcttcgt gctttttcta tttcaataaa
    28081 acaagttctt ctattttgat ttttgctact tctgcaagta gtgcttatgt gctaaagtaa
    28141 gaatgaaaag gagagataaa catgatattt gattctcata cccatttaaa tgctgaacag
    28201 ttcaatgaag atattccaga aacgattcag cgagcacaag aaatgggggt aacgaaaatg
    28261 gcggtcgtgg gcttcgatac gccaacgatt gaaaagtctt tgcaattaag ccatgactat
    28321 ccaaatatat acagtattat cggctggcat ccgacagagg ctggcagtta tacgaaagaa
    28381 atcgaaaata aattacaaga acaattgact ttaccaaagg ttgtagcatt aggggaaatt
    28441 ggcttggatt atcattggat ggaagacccg aaagaagtcc aagataaagt atttcgcaga
    28501 cagattgcta tcgcaaaaga aatgaaccta ccaatcagta ttcatactag agaagcactt
    28561 gaagacacgt atcaaatcct aaaagaagaa gatgttcgag atatcggtgg aatcatgcat
    28621 agtttcagtg gtgattatga atgggcaaaa cgatttcttg acttagggat gcatatttca
    28681 tttagcggtg tcgtcacttt caaaaaagca ttagacgtcc aagaggctgc agcaaatgta
    28741 ccaatcgaac gattacttgt ggaaacagat gccccttatc tggcaccagt tccttttaga
    28801 ggaaagcgaa atgaaccagg ctatactcgt tatacagtag aaaagattgc tgaacttcgg
    28861 aatcttccga cagaagaagt ggctgcccaa acatggacaa atgcgcaccg attatttggt
    28921 ctaagcgaac atggataaga tgaagatcca agagattata gtcgttgaag gaaaagacga
    28981 tacaagaagg ctgcgggaag tagttgatgc agatacgata gaaacaatag ggtctgcaat
    29041 caatgaagag atcctgatgc aaattgaaca tgcccaagag actcgtggag tgatcgtttt
    29101 taccgatcct gatttttcag gagaaaaaat aagaaaaatc attatggaag ctgttcctca
    29161 ggcaaaacat gcttttttgc cgcgcacaca agctaaacca aagaaaaagg gaagccttgg
    29221 tgtcgagcat gcaagtgatg ctgctatttt agaagcattg aaaaaggtcg tgacgccaga
    29281 catttcccaa acggctgttt ctgaaatcac tagacaagac cttttagact atggtttaat
    29341 agctggggca caagcaaaag aaaaacgaga aaaactaggt gacgagttgc gcatcggtta
    29401 cacgaacggc aaacaattgg aaaaacgttt gaagatgttt cgtattacgc cgaaagaact
    29461 cgcccaagca atgaaaatag tggaggaaca acatgagtga atatcgtgaa attgcgacac
    29521 cctctagaac aaaagagatt ttattgaaac atggtttttc atttaaaaaa agtttaggac
    29581 aaaattttct gactgaacca aatattttaa ggaaaatcgt agaaactgcg caaatagatg
    29641 aacatacgaa cgtgatcgaa gttggaccag gaatcggtgc attgacggaa caattagcca
    29701 agcatgcaaa acaagtcgta gcatttgaga tcgatgatcg tttgatccct gtattggctg
    29761 atacgatgca accctatgac aatgtgacta ttgtacatca agatatttta aagaccgatt
    29821 tgtcaacagc tgttagagaa acatttcatg aggagcttcc gttaaaagtc gtagcgaatt
    29881 taccttacta tattacgaca ccgatcatga tgcattttct tgaatcagat ttggttgtgg
    29941 atgaactagt cgtcatgatg caaaaagaag tagctgatcg tatctctgct gaaccaggaa
    30001 cgaaagctta tggttcatta tcgattgctg ttcaatatta tatggaagcc agtttagcat
    30061 tcatcgtacc aaaaacagta tttgtccctc agccaaatgt agattcagcg attttgaaac
    30121 tgacaagaag agatataccg gcagtcgaag tgacagacga aaaagcattt ttccgattga
    30181 caaaagcggc atttcagcaa agaagaaaaa cgctttggaa taacttgcag cattcgtatg
    30241 gcaaagatga ccagacaaaa gcatggttag caaaaagctt ggaaacagct ggaatcgatc
    30301 caaaacgtcg tggggagact ttgtcattac aagaattcgc cgcattaagc aatgcgatgt
    30361 cagaaaatat gcagtaaaaa aagaggttgt gacagaagtt gtgaaaaagc tgtcctgcat
    30421 caagtaataa gaacagccaa acaaaaatag ttcctcatgt ttttcgtttg tttgggctta
    30481 ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga ggttgtgggc ctgccgttta
    30541 actctaagtc acaaggaaca atttcaaaaa acagcttctc atgtttttga gttagttggt
    30601 cttattactt gatgcaagct tttgaccact attatgccga acaaggagat gtgacacaaa
    30661 ttttgtcaca tctcctattt ttgtttcttg atcaaaaaat tcttatactt gatactcaca
    30721 ctcattttgt atggatttag aaaagaatat gtgtcttgac agttattagt aagtgagtta
    30781 tactaagtcg acatttatgt gtcaagacat attaggagag agaaaaaatg agaaaaaatt
    30841 cagttcgaaa actaacgatt tctgctttat tgattgggat gggggtcatt atcccaatgg
    30901 tcatgcctaa aatcatgatt ccaccagctt cctttacttt ggcgtctcat gtgccattat
    30961 ttattgcgat gttttttaca ccaggagttg cagtggctgt tgcgctaggt acgacatttg
    31021 gtttcttttt aacaacgccg gttatcatcg cattgcgagc attgtcacat cttgtgtttg
    31081 cgttgattgg ggcttggtac ttgcaaaagc atccgagtat cgttttaaaa aatggacagt
    31141 ttacttttgc taattggcgt ttccaagggt tcaattttgt gatcggtgtg attcattctt
    31201 tagcagaaat gctagtagtc agcatctttt actttattgg taatatgcct gaaacatatt
    31261 attctcaagg atatttttat acagtattca tcttgatggg gattggtggg cttatccaca
    31321 gtttagttga ttataatatt gcttatttta tagcaggaac actcagcaaa tattttgata
    31381 tccccgtatt tacggcagca aaaaaagaaa atcggaaaga gtcgattcaa gggtacgcga
    31441 agaaaacgac atattagaca tgatctctca aaaaaaaaac tctcatctag taatggataa
    31501 taaatcctct aagatgagag ttttttgtcc taaaaagagc tgcaccaaca tcttctggca
    31561 cagctctaac agggggcaat ttatggaagt ttgatgactt gttctgtttc tttatcaaag
    31621 aagtggcctt tgctgatgtt gaaggcaagg tcgactattt cgcctggttt atggaaatct
    31681 cttgcatcta cttttgagat gaactcagta gaaccagttt ttgtgtaaag catcgtttca
    31741 gctccaagta attcggaaac gacaacttct gatttcacta cggcttcagg tgaagcttca
    31801 agggctactt gttcactgtg gatgtcttct ggacgaatac cgaagataac ttcttttcct
    31861 tcataacctt tttcaactaa tactttgttt tttccttcag gaatacgaag cttaagccca
    31921 tgtccatctg tgataacacc atttttcaat gtcacattaa agaagttcat ggcaggagat
    31981 ccaataaagc cggcaacgaa catattgacg ggtgtatcat aaacttcttt cggtgtaccg
    32041 atttgttgga taaatccgtc tttcatgata acgattcggt cagccatagt cattgcttct
    32101 gtctgatcgt gagtaacata gatggtcgtt gtttcaagac gctggtgaag tttggcaatc
    32161 tcagcacgca tggctacacg taatttagca tctaaatttg ataatggctc atccattaag
    32221 aagacttttg catcccgaac gattgcccgt cccaatgcca cacgttggcg ttgtccacca
    32281 gataaagcag ctggttttcg tttcaagtat tctgtcagac ctaagatctc tgctgcattg
    32341 tccacacgtt ttttgatgtc tgctttatca tatttacgta atttcaagcc aaaagccata
    32401 ttatcaaaaa cagtcatatg aggatacaat gcgtagtttt ggaaaaccat ggcaatatca
    32461 cggtctttag gtgccacgtc attcatgact tttccgccga tatttagttc tccttcagag
    32521 atatcttcca gtcccgcgat cattcgcaaa gtagttgact ttccacaacc ggaaggacca
    32581 acaaaaacga taaattcacg gtctttgatt gatagattaa aatcagttac agaataattt
    32641 tctgcattgt catatttttt atatacgttt ttcagagcca tttctaccat aatacgttcc
    32701 acactttctt ttttagtttt attaactatt taaagtataa atgaaagcgt taactaactt
    32761 caatgtcatg ttgaacaaga attttttctt ttttttgtca atgtgctagt tttaagcttt
    32821 aataaatatc cataagaaat aaaaactgat acactggagt aaataagtat gttaacaata
    32881 tcaaaaggag tgtctcttat gaaaatcgct ttgatcgctc atgaccgaaa aaaacctatg
    32941 atggtgaaat tagcaacagc gtataaaaca atattggaaa aacatgaact ttttgctacc
    33001 gggacgactg ggcagaggat cacggaagca accggcttgc cgatccatcg gtttaaatcc
    33061 ggaccgttag gcggggatca gcaaattgga gccatgattt cggaggatca gttagatttg
    33121 gtgatttttc ttcgagaccc gctggctgca cagccccatg aaccagatgt aacagcactt
    33181 atccgtttat ctgatgtcta tgagattccg ttagctacga atatcggaac agctgaggtg
    33241 ctactaagag gactggaagc cggcttccta gattttcgaa gaatcgtcca tgaaatagac
    33301 aagcatccac tttcattttg atggataagc gtatggaaaa ataacgttta atgttcgcct
    33361 atatgtagct ataaaagcta aacaaactga taatatgcga tataatacga acgtattgga
    33421 taatataaac aataatatta gtttttcgta tttgcagtaa atgtgaaaat atgctatagt
    33481 ttctgtggga aaagatccca aaaataatac agagtagaaa ataaagcgtt aagtgctaag
    33541 aggatgggat gttgcctctt ggacgaagga cgtaatgttc gcggttttat tttcgcattc
    33601 gctgcatgat acgatgtagc agctcttaaa ggagatgcta aaaatgaaga aacaccggtt
    33661 agacgcacgt atgattgcta ttatgggctt attgattgca ttgatggtca ctttgtcccg
    33721 actggtagca atcgagaccc catttattaa aatcagtgtc acatttattc cacaagtgat
    33781 catgggcatt ctttttggtc ctttttggtc agggataggc gcagttttag ctgatttagt
    33841 aggaatggcc ctcttctcga aatctgcatt ctttattgga tttacattga atgcatttat
    33901 tgaaggagca atctacggat tctttttcta tcgaaaagag atcacttgga aaaatgccat
    33961 tctggctacg cttagtgtca cgctgatcat caacttattt ttgacaccat tatggcttgc
    34021 gttgatgtac catgtgccat tgttcagctg ggtcgtttgg gcgccccgcc tgttaaaaac
    34081 agtaatatgg ttaccgattc aatcaattgc catttattac gtaggacgta gtattcctta
    34141 caaaaaaatt ttaagaagtt tagcgatcca cgctaaataa aaa
//
LOCUS       DACDRN010000022        33769 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDRN010000022 DACDRN010000000
VERSION     DACDRN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.022"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(86..1360)
                     /locus_tag="IWJ80_001305"
     CDS             complement(86..1360)
                     /locus_tag="IWJ80_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9461047.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            1633..4104
                     /locus_tag="IWJ80_001306"
     CDS             1633..4104
                     /locus_tag="IWJ80_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9461048.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(4207..5943)
                     /locus_tag="IWJ80_001307"
     CDS             complement(4207..5943)
                     /locus_tag="IWJ80_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP9461049.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5940..6644)
                     /locus_tag="IWJ80_001308"
     CDS             complement(5940..6644)
                     /locus_tag="IWJ80_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9461050.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6775..7278
                     /locus_tag="IWJ80_001309"
     CDS             6775..7278
                     /locus_tag="IWJ80_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP9461051.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7238..7576
                     /locus_tag="IWJ80_001310"
     CDS             7238..7576
                     /locus_tag="IWJ80_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP9461052.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7597..9015
                     /gene="ffh"
                     /locus_tag="IWJ80_001311"
     CDS             7597..9015
                     /gene="ffh"
                     /locus_tag="IWJ80_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP9461053.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9127..9705
                     /locus_tag="IWJ80_001312"
     CDS             9127..9705
                     /locus_tag="IWJ80_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP9461054.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            9709..10230
                     /locus_tag="IWJ80_001313"
     CDS             9709..10230
                     /locus_tag="IWJ80_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9461055.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10309..10863)
                     /locus_tag="IWJ80_001314"
     CDS             complement(10309..10863)
                     /locus_tag="IWJ80_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9461056.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11116..11391
                     /gene="rpsP"
                     /locus_tag="IWJ80_001315"
     CDS             11116..11391
                     /gene="rpsP"
                     /locus_tag="IWJ80_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP9461057.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11403..11654
                     /locus_tag="IWJ80_001316"
     CDS             11403..11654
                     /locus_tag="IWJ80_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9461058.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11779..12570
                     /locus_tag="IWJ80_001317"
     CDS             11779..12570
                     /locus_tag="IWJ80_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461059.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12740..13261
                     /gene="rimM"
                     /locus_tag="IWJ80_001318"
     CDS             12740..13261
                     /gene="rimM"
                     /locus_tag="IWJ80_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP9461060.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13251..14012
                     /gene="trmD"
                     /locus_tag="IWJ80_001319"
     CDS             13251..14012
                     /gene="trmD"
                     /locus_tag="IWJ80_001319"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP9461061.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14148..14495
                     /gene="rplS"
                     /locus_tag="IWJ80_001320"
     CDS             14148..14495
                     /gene="rplS"
                     /locus_tag="IWJ80_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP9461062.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            14753..15046
                     /locus_tag="IWJ80_001321"
     CDS             14753..15046
                     /locus_tag="IWJ80_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP9461063.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            15889..18216
                     /locus_tag="IWJ80_001322"
     CDS             15889..18216
                     /locus_tag="IWJ80_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9461064.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            18342..18671
                     /locus_tag="IWJ80_001323"
     CDS             18342..18671
                     /locus_tag="IWJ80_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9461065.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            18711..19715
                     /locus_tag="IWJ80_001324"
     CDS             18711..19715
                     /locus_tag="IWJ80_001324"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9461066.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            19712..21141
                     /locus_tag="IWJ80_001325"
                     /pseudo
     CDS             19712..21141
                     /locus_tag="IWJ80_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            21347..22519
                     /locus_tag="IWJ80_001326"
     CDS             21347..22519
                     /locus_tag="IWJ80_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9461067.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(22681..23136)
                     /locus_tag="IWJ80_001327"
     CDS             complement(22681..23136)
                     /locus_tag="IWJ80_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461068.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            23726..24148
                     /locus_tag="IWJ80_001328"
     CDS             23726..24148
                     /locus_tag="IWJ80_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9461069.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(24314..25507)
                     /locus_tag="IWJ80_001329"
     CDS             complement(24314..25507)
                     /locus_tag="IWJ80_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9461070.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            25731..26108
                     /locus_tag="IWJ80_001330"
     CDS             25731..26108
                     /locus_tag="IWJ80_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9461071.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            26096..26434
                     /locus_tag="IWJ80_001331"
     CDS             26096..26434
                     /locus_tag="IWJ80_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9461072.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            26568..27011
                     /locus_tag="IWJ80_001332"
     CDS             26568..27011
                     /locus_tag="IWJ80_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP9461073.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            27273..28088
                     /locus_tag="IWJ80_001333"
     CDS             27273..28088
                     /locus_tag="IWJ80_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9461074.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            28098..28760
                     /locus_tag="IWJ80_001334"
     CDS             28098..28760
                     /locus_tag="IWJ80_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9461075.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            28965..30629
                     /gene="alsS"
                     /locus_tag="IWJ80_001335"
     CDS             28965..30629
                     /gene="alsS"
                     /locus_tag="IWJ80_001335"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP9461076.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            30642..31352
                     /gene="budA"
                     /locus_tag="IWJ80_001336"
     CDS             30642..31352
                     /gene="budA"
                     /locus_tag="IWJ80_001336"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP9461077.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            31482..31979
                     /locus_tag="IWJ80_001337"
     CDS             31482..31979
                     /locus_tag="IWJ80_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP9461078.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            32164..32424
                     /locus_tag="IWJ80_001338"
     CDS             32164..32424
                     /locus_tag="IWJ80_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461079.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(32786..33025)
                     /locus_tag="IWJ80_001339"
     CDS             complement(32786..33025)
                     /locus_tag="IWJ80_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461080.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            33311..33625
                     /locus_tag="IWJ80_001340"
     CDS             33311..33625
                     /locus_tag="IWJ80_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461081.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT        11319 a         5956 c         6752 g         9742 t
ORIGIN      
        1 gctcttcgtc aaatcgtgtt ggtgaagaca aatttgaata aaacttagtc cgatatgcgg
       61 cgcgttctgt gcgccgcata tttttttagt tttcaaagat cagaccttgt tgtaaataga
      121 tccgtgcccg aaagttataa aagttgcgat aaccaaacgc aattcgttta atcagcttga
      181 tcttgttatt cagcccctca gtgacgccgt ttgagtggga caccttaaag gcatttgaaa
      241 tacccgattg gtagcaatgg aagatcgtga atttcttttt aaacgactga ggtaacgtag
      301 atgaaattgt tttaagggtt tcttgaaagc cttccgtacc tcgtttaacg aaataatagc
      361 gtaataattg acaaaaatga tacgcagttc gcaattcttc gttgtagctt aacagctcat
      421 caacgatttc tgtttgacta attggtcgtt taaacagtcg atgataatgc cgtttgagtg
      481 gctcccatgt cgtggaatct ttgagtaaga gtttccaata tcgcttgagc cgacggtact
      541 tcttctgatt ttcagagtgt gaggttcgaa aacgattcat gatctggaca cgtaattgat
      601 tgaagctgcg attgatgtgt tggacaatat gaaagcggtc agttacgatc tccgcatttg
      661 gaaagactgt tttaagcaac tgacaatagc tggcattcat gtccatcact aggtacttga
      721 cctttaaacg ggcctttcta ggaaagcgca ggaagtattg agttagtttg taaagtctgc
      781 gatcttcaag gatcgtaaat agctgattgg ttgtcccatc aacacaaata aaactcatgg
      841 aaccagaaac agaattcatt gatttgaact catccataca caacaccgtc ggaaggaagt
      901 tcatacgtgg ctggtaatgc ttcgttgctt cacgcaagat ccgtgtcacc gtcccatccg
      961 agacgaagta cttcttagcg attagtttac gtgattgatt ttcgtacagt tccatgagaa
     1021 tcgcctgctt cagttccttt gttagttgat gatgttcatc aactaagtcc gttttcgcag
     1081 aaaaggtcct tccacaatca tggcagagaa aacgggtacg ttttaattct aaaatagtta
     1141 acttttcttt gactttcaac atctgagtgc gtgtcgtata ggaaccgttg cggataatac
     1201 gaaccgatcg acagtgttca caataggaag aagagtcatc caattttcct ttaataaact
     1261 gcgcttgaac gcctttatat tctttttcaa tcagccattc ttcatcaaaa aataaatgtt
     1321 tatctgttaa tccgagtaat tttctagtat gattatccat tgagagactc ctttggtagg
     1381 atgacgtttt ggtcgactaa attttaccaa aagtgagtct ctttttgagt tgaaacgaag
     1441 aaaaagtgtt agtagaaaat cgccttcgat tttccaccaa cacaaaatat tatagaaccc
     1501 tttttttttg ctatatgaca ggaatgaaag gtgtttgcaa gcttaatgtg aggaacactt
     1561 tccatatctg ttctatactg gataggagaa gtttggaaat atccagaaaa cgaaatcaat
     1621 cgaggtagcc acatgaagaa taaatggata aatcgaagta aaaaaaagaa agtaacacaa
     1681 agatcatcag ataaagtcat ccgtcttttt ccatcgtata aagaaggatt aacggcagat
     1741 caagtcaagg aacgaataga aaaaggtgca gcaaataatt cagtcgatcc cacattcaaa
     1801 acaaatcagc aaatcgtatt ggaaaacata tttacgtatt tcaatttgat ctttcttatc
     1861 ttagccattt tgctttgttt agtagaatcc tataagaact tgactttttt accagttatc
     1921 atagccaata caggaatcgc catttttcag gaaattcgat ccaaaaagat cttggacgaa
     1981 ctaaatgttt taaatacagc aaaagtcaat gtcatacgag acgggataga acaaacaata
     2041 gatatagaag accttgtaat agatgacatt gttattttag agactggata tcaaattcca
     2101 gcagatgcag aggtcgtgga agggaaactg caagttaatg aggctttatt gacaggcgaa
     2161 gcagatgaaa taaccaaaga aataggtgat ccattaatgt ccggcagttt cattgtttcc
     2221 ggaaaagctt acgcccgatt agacaaagtt ggagcagatt cgtatatttc ccagctgaca
     2281 gtcaaagcca agacaatggg agaaggagaa cagtcggaga tgattgcttc tcttaatcaa
     2341 ttgatcaaat ggattgggat catcattatt cctatcggta tcctgctttt ttctcaaagt
     2401 tactttttta atggaaacac gatcaaagaa agtgtcgttg caatggaagc agcattgatc
     2461 ggtatgatac ctgaaggact gtacttgctg acgacgattg cattagcatt aagtgcgact
     2521 aaactagcaa agcaaagagt tcttttacac aatatgaaaa gcattgagac attggcaagg
     2581 gtaaatgtcc tttgtgtaga taaaacagga acgatcacag aaaataaaat gtctgttcaa
     2641 aaaatgatcg tctctaaaaa acaagaaata atcagtcagt cagatcaatt ggaagcatgc
     2701 attgctgact atgcaaaagc gatggcagga gacaatgcaa caatggaagc tgttaaagca
     2761 tattttacaa aaaccactga tcaatcctat caccatgtta ttcctttttc ttctgtccaa
     2821 aaattcagca gtgtagcgtt ggcagacaag gtgtatgttt taggagcgcc cgaaatggtt
     2881 ttgcgcgatc aattgccgga atatgcggaa gagtttgttt ctttcgctga aaaaggctat
     2941 cgcgtgttgg tctttggtgt gtacgatggg attcttgaag aaccagtatt gacagaagca
     3001 gtcacaccat taggctatat tctgattgct aatcctatca gaaaagaagc aagagccaca
     3061 tttgattact tcaaaaaaca gcaagtagcc atcaaagtga tttctggaga taatccgctc
     3121 accgtatcca atgtagctgt ccaagcaggg atttcacatg ctgatcagta cgttgatgca
     3181 tctaaattat cagaagaaga atatgaagca gctgtcgaaa aatatacagt ttttggacgc
     3241 gtcaagccag aacaaaagaa aatcttcgtt caattgataa agcgtaacaa tacagtagct
     3301 atgacaggtg atggcgtgaa tgatattcta gccatgaaag aagcagactg cagtatagcg
     3361 atggcttccg gaaatgaagc cgccatgcag gcctcacaag tggtcttact tgattctgac
     3421 ttttcacgca tgccagaagt tgttgcagaa ggacggcgcg ttgtgaacaa tattgagcga
     3481 tcagccagct tgtttttagt gaagaatatt ttctcctttt tgctctcact gttttcagtg
     3541 atctttgctc tgacctatcc tttagaacca tcacaaatca cattgattag tttgtttact
     3601 atcggacttc catctttctt gcttgctcta gaagaaaaca aaaagcgaat cagagggaaa
     3661 ttcatcatga atgtgatgga aaaagctgtg ccaggtggac taacagatat gatcgtagtc
     3721 ggtgcgttgg tgatctgcgg ggtgactttg aatctaaata aaacagatgt ttctacagca
     3781 tctaccatgc ttttgattgc agtagggttc ctcgttctct ataaaatctg ttcaccgctg
     3841 aataaattca gaagtcagat cattcttttc tgtgctagcg gtaacttttt ttcggtcatc
     3901 tttttgcata agctgttttc aatcaccggt atctcagctg tatcactttt gctgttgagc
     3961 attctatttt tctcggcaga ttcgattttt aggcatttga ccacattggt agaatatttg
     4021 tttgaacgta aaaatgaaaa agagcaaaag ccgtttagtc gcgtagctaa aaaatttcta
     4081 tcgatattta aacgagaaaa ttgacgaaaa gtatctcaaa aaagagtaaa aaagaacaac
     4141 cactcgtgta tctttcgaag caggatgagt catccaactt taaaacgcac gagcagttgc
     4201 tcttttttac attttcggaa tggtaacggt gaaagtcgaa cctgttccaa gatgactgtc
     4261 tacagtaatc gtaccattaa ggagttgcac gtagtctttt acaatcgcta accctaatcc
     4321 ggtcccgcct gaatgtcggc ttcgcgcctt gtctacgcgg taaaaacgct caaagatccg
     4381 ttcctgatcc tcaatgtcga tacctatacc aaagtcttgg acagaaaacg atagatcatc
     4441 tgttgcctga tagcggataa taatctcgct gtcgcttttt gaatattgga cagcattttc
     4501 aatcagattt ttgataattg gataaaggat atctgtttta gtagtaaatg gttcgttctt
     4561 tccaatcaat cgaatcgtca accgtttctt ttcgatgatt ggctgataat tttgaacgat
     4621 ttgctggaaa taaggttctg tataaattgt ctggatctcg taagaattct cgccatcttt
     4681 tgacagctgg atgatttccc gaatcagttt atctaaccgg atcgcatctt tttgcatgat
     4741 ctcaagaaaa gaagtcagag tttgcgggtc atcttttgca ccatccagta aagtctcggt
     4801 aaaaccaatc agtgaagtca caggagtttt taattcgtga gagacgttgc caacgaaatc
     4861 cttttgtagc ttttctaaac gtcgcacttt cgttaagtca tacgccaccc ctagaatctg
     4921 tccggtacct tctgaattag taaagtagcg caaactcaag tctaatgtct gccgaccttc
     4981 tgtaagggtg atctcctgat ggatcagcgg atgctcagga gtcacttgat gaatcagctg
     5041 aatgagtttt ggctcttgaa tgacttctgt gtaccgctgt tcaggttgat agcttaccac
     5101 gtttaaatgt agctgcatct ttggattaag cagtagtaaa cgagaatcag catcatcaat
     5161 caggaatacg ccaatcatca gttcgttcag taacgtatat aattgctctt ctgtggtagt
     5221 acacgcccga tacatcttac tcatttgttc gctcaacgca ttaatcgtct gatacaattc
     5281 ttcccattga tcgggtgttt gcatgattac ttccgcctga ttaggatttt gcaccatttt
     5341 ttttagtacg ggcagcacgg ttttcaatgg ctggtttttt tgatagatca gataatagat
     5401 cattcctgca agaacaagga agaaagacag aaagaagaaa aagacccaac ggcgaaaact
     5461 ctcagttctc ggtaaaaaaa cgcttgtcgg ttctgcgata cgtaaaatgg cttctaactg
     5521 gccacttttt ttgactggta atgcgacata taaaagatcc tcattcaaag tagtactttt
     5581 gcgcaaagcg ctacctaggt tcccaccatt caatacggct ttgatttcgg gacgggagtt
     5641 tcgctgttca ttcaatgttt cattgtttgt gtcaaataag atctttccag tagcatccat
     5701 taaagtaatc cgttcgttcg actgatggac gaattcttcc aaagcagttt tgttttgcgc
     5761 tgcttggagg ttttcgacat ctaattgccg gatcagcagt tgtgcttttt ttgtcaggta
     5821 gctttcttgc tgttccacga tctgctgttg aaaataatgg gaaatcaatt gccagccacc
     5881 aaaaaacaaa ctaactaaaa gaataagaaa gaagagacgt tcataaattt ttttccattt
     5941 catcgtttgg gttcctgaaa cttatagcca aatcctctaa tagtcaccaa gtactttggg
     6001 tgttttggat ctcgctcgat tttttctcgc aaatgactaa cgtgtacatc tacgattcgg
     6061 ctttgtcctg cgaagtcaaa attccaaatg cggtctagca atgtgtctcg gtcgatcaca
     6121 cggtcttttc gtttcatgaa ataaacaagt aattcaaatt ctttcggtgt tagttcaatc
     6181 agatggtcgt ctacggtcac ttgataattg gtcaaatcag cagtgatttg tccaatcgat
     6241 agatattcag gctcagcttc atcttttgat tgcttacgag gttcaattcg tctgaatatt
     6301 gctttcatac gggctaatac ttcgcgagga ctaaatggtt tggtcaaata gtcgtcagca
     6361 cctatttcta ggccaatgat ccgatccact tggtcatctt tagccgtcag catcaaaatc
     6421 ggtgtttcat ttttttcttg acgcagccgc tgagtgatct ccatgccatc catcgttgga
     6481 agcatcacat ctaaaatgat gaaatcaaat gattgttcta atgctaattc taatccttcg
     6541 cctccatctg tggcactggt taccttgtag ccttcttttt ccaaattgaa cgtcaagagc
     6601 gttacaatag aaggttcatc atcaacaact agtatttttt tcattcaaat ctcctttaaa
     6661 taggtttcat tctgtttata ttttagcata gaatcagtac aaagggctat tttacaaaag
     6721 aaataaatgg tataatagga aggcactttg ctgaatagaa aggaagagca gtcaatgatt
     6781 ggaatcagtg catgtttagg cggtctggcc tgtcgctatg atggacagaa aaaagaagta
     6841 ccagaattga tggaactagt tgaaactgga gaagctgtca tggtctgtcc agaagtgatc
     6901 ggcggattac caattcctag agatcccgct gaaatcattg gtggaaatgg attcgatgtg
     6961 tggaaagatc aagcaaaagt atggacgaca agcggcgaag atgtaacgga agcgtacaaa
     7021 aatggcgcga tcaaagccta tcaaaaattg caggaaaaac acattgatct agtcatcatg
     7081 aaggaaaaaa gcccttcatg cggaacccat tcgatttatg acggaagttt ttccggaata
     7141 aaaaaaaatg gtgccggagt ggctactgct tattttattc agcaaaaaat gactgtgctc
     7201 tctgatgaag aatggctgaa aactagaggt gagcagaatg gaaatcgaaa agaccaatcg
     7261 aatgaacgcg ctctttgaat tttattcgac cttattgaca gaaaaacaaa tgaactacat
     7321 ggaattatat tatgcagatg atttttcatt aggtgagatc gcggaagaat acgaagtaag
     7381 ccgacaagca gtatatgaca atatcaagcg gacaagcaag atcttggagg attatgaaaa
     7441 aaaactccat cttttttcta attatattgt tcgagaacag gtattggaaa atatgactgc
     7501 atatattaca gataagtacc cggaagataa aaaactactt gaatatgtac agcaaattca
     7561 agcaatcgaa gaataaataa aaggaatgaa gaaaatatgg catttgaaag cttaactgaa
     7621 cgtctccagc aggcgatggg aaaaataaga aaaaaaggaa aagtatcaga agctgacgta
     7681 aaagaaatga tgcgggaaat ccgccttgcc ttactagaag ccgatgttaa cttgcaagta
     7741 gtcaaagatt ttactaagcg cgtacgcgaa cgtgcggtag gggcagaagt gctggaaagt
     7801 ttatcacctg cacaacaaat cgtaaaaatc gttgatgaag aattgacgat cacacttggt
     7861 tctgaaacag cagagttaaa taaatcacca aaaataccaa ctgttatcat gatggctggt
     7921 cttcaagggg caggtaaaac gacctttact ggtaaattag ccaattattt aaagaaaaat
     7981 gaaaatgctc gtccgctcct aatcgcaggg gacgtatatc gaccagctgc gatcgaccaa
     8041 ttgaaagtat taggtcaaca gctagacgtt cctgtatttg atatgggaac tgaagtcagc
     8101 ccggtagaga tcgtccgtca agggatggag ttagcaaaag aaaagaaaaa cgactatgta
     8161 ttgatcgata cggcaggacg tctgcatatc gatgaaacat tgatggatga gttgaaacaa
     8221 atcaaagaat tgacgcaacc aaatgagatt ttgcttgttg tcgatgccat gactggtcaa
     8281 gatgccgtca acgtagctga tagcttcaac cagcaattag gtattacagg ggtcgtcatc
     8341 actaaacttg atggggatac tcgaggcggg gcagcactat cgatccgctc agtgacaggc
     8401 gcaccaatca aattcatcgg ttctggtgaa aaattgactg acttagaagt attccaccca
     8461 gatcgtatgg ctagccgtat ccttggtatg ggggatatgc tgacattgat tgaaaaagcg
     8521 cagcaagact acgacgagaa aaaagcggaa gaacttgcac agaaaatgcg ggaaaattca
     8581 ttcgatttca atgactttat tgaacagctt gatcaagtga tgggtatggg cccactggaa
     8641 gatctaatca aaatgatccc aggaatgaat caagtccctg gaatcgaaaa cgtcaaggta
     8701 gatccaaaag atgttgaacg caaaaaagcg atggtttatt ctatgacgcc agcagaaaga
     8761 gcaaatcctg atctgttgaa cccaagccgt cgtcgccgaa ttgctgccgg ttctggaaac
     8821 agtgttgttg aagtgaatcg aatgatcaaa caattcaagg aatcaagaaa aatgatgcag
     8881 caaatgtcca aaggggatat gaacatccca ggtatggacc aaatgtttgg ttcaggcgtc
     8941 aaaggaaaac taggcaaaat ggcgatgaat cgaatgatca agaaaaataa gaagaaaaag
     9001 aaaaaacgga aataagatga aaaaaacagc ttcggtacag actgaaaatg tctactgccg
     9061 gagcttttct atcagcaatt tttattcttt actaatacaa ttactctaaa cgaagaggtg
     9121 gcacagatga gatgtccgtg gagtgaacaa acagaaagta tgcggcaata tcatgatact
     9181 gtctggggtg ttcctgaata tgatgaccaa aagctgttcc gaaaattgat gcttgatatt
     9241 aatcaagccg gattgagctg gcagacgatt ttaaataaaa gtgaagcatt cgatgaagca
     9301 tatgatcgat tcgatatcaa aaaagtcgca aattatggag aaacaaaaat cagtgaacta
     9361 atggaaaaca aaggaattat tcgtaaccga agaaaaatcg aagcagccgt ccaaaacgcc
     9421 aaagcagtct tgaaaattca agaagaaatc ggcagttttt cagagtatct ttggtcgttt
     9481 tctaatggaa aaatcgttga ttcttcttat cattcacaag aagaagtccc aacaacaagt
     9541 cctctttcag atgaattagc aaaagatcta aagaaaagag gatttaaatt tatcggaagc
     9601 acaacaatct atgctttttt agaagctgtc gggatcatca atgatcacct tgacagttgt
     9661 ttccgcaaag aagaggtcgt acgtatcggc agaaagggcg aataaagcat gcgaaaaatc
     9721 atattttatg gtgctataag tttagatggc tatttggcaa cgaaaacgga tgatcttcaa
     9781 tggctttttg acactccaac aggtaagcaa actacttatg aagcttttta tgaaacaatc
     9841 gatacgacca tcatgggcag aaaaacttat caggaagcaa aaaaatattt ggagaccgaa
     9901 gcaatttatc cagagaaaaa gaatttcgtt ttttccaccc acgcacaatt acaattgcca
     9961 gatgctgagg tcgtaaacga agatcctgtt gcttttctaa aaaatctaca aatgacagaa
    10021 ggaaaagata tctgggttgt gggtggcggt cgattgatca aaccgctatt ggaacatcaa
    10081 atgattgatg aatggtatat ccagattacc cctgtattat taggggacgg gattccttta
    10141 ttccaaaaag gaacatatga agcgcgtttt gaaatgctag acacgacacg attcggtgaa
    10201 tttatcgaat tgcattatgt acgaaagtag aagtaaataa aataaaaaag gcaactatat
    10261 gcagaaatgt cctgtacata gttgcctttt ttattaccgt tatgcttttt actctgttaa
    10321 tttgttagaa aaatctagat acgcatctaa aatcgtttgg aagaaactta atgttccttc
    10381 aacgatatta ccattttcat caattagatt cgcaacgttt ccgatatatg cttccggttg
    10441 ctgcaaggtt ggcacattca aaaagacaag tgattggcgt agatggtggt ttgcgccaaa
    10501 gccgccgatc ccacctggtg atgcagtaac gactaaacct ggtttatgat cccaaacact
    10561 gtgtccataa ggtcttgagc ctacatctaa agcatttttc aatactgctg gcacagaacg
    10621 gttgtattca ggagtaacga aaacgacacc gtctagtccc ctcacttcct cacgaaaacg
    10681 agtccattca gcaggagcct gttctggagt ttcgagatcc tcattgtaaa atggcagatc
    10741 atcgattttt acaaatacac tttcgtaccc ttcaggaagc aatttcgcaa aagcttctgc
    10801 tacttttttg ttgtaagaat cttttcttaa actaccaata aaaaatccaa ttttttttgt
    10861 catacgattt ttcctccttc aaactatcta taataatctt acaaaagata agtaactgga
    10921 gcaaataaaa acacttactg acaaaagcta catagaagat agattactta tttttaacaa
    10981 aaaagaagtc taaaatcttc tattttctaa aaaaaatcga atttaatcaa gctgtaaaga
    11041 aaaaaagctt tacagggtaa aaaaaaactg ttacactaca tcttgtgaat aaaacaatgg
    11101 aggtggaaat aaataatggc agttaaaatc cgtttaaaac gtatgggttc taaaaagagt
    11161 ccgttttacc gtatcgtcgt agctgattca cgttctcctc gtgatggacg tttcatcgaa
    11221 actgtaggta cttacaatcc tttgaaagac cctgcagaag tagttttaaa agaagactta
    11281 gttcttgact ggttatcaaa aggtgcacaa ccttctgaca ctgtacgtaa catcctttca
    11341 aaagaaggcg ttatgcaaaa acaccacgaa gctaaattct caaagaaata aggtgaccgg
    11401 atatgaaaga tttaagcgac ttagtcttaa caatcgttcg tccgctagtc acttatcctg
    11461 atcaagtcaa gttagacgtc gtagagtcga aagactttta cgagtacaat ctaacagtgg
    11521 ctcctgaaga tattggccgt atcattggaa aacaagggcg tgttgctaaa gcaatccgca
    11581 caatcgttta cggtgttcgg atcaatgcac cgaaaaaagt gcgtttgaat atcatcgatg
    11641 ataaggaaaa gtaaataaac gtgctgtact cgaattttcg agtgcagctt ttttgttttt
    11701 tttattaatt gaagaaatga ttatttggca aaacaagcaa gaaagcgtat ccttatagga
    11761 ggagggagtg aacagccaat gagaaagaaa atgttattta ttccatttgc tctattgtta
    11821 agcgcttgta gttcggttac tcaagaatca gacaatacac atctatcaag cagtatatct
    11881 gaaactgtta ccagtccaga aaaaagtagt accaaaacgg cttccaccac tgaacaaaca
    11941 atggctacct caaataatga gaaaaagact gctttagacc agttgaaaga acaacagcca
    12001 aatgttccca tgcctctaga tgtaccagtc tcttcaggct atctcaatat cgcagcgaca
    12061 catacaaagc aaggttattc tattttatat tacagaacag atcgtcccct cgggttgaat
    12121 gctgacgaat tgaatcaaga aacgcctatt gcaacctatc tatatcaata tgggtttgct
    12181 tctagtcaag aaacaatcca agttttgcag ccatttgaga tcgacacaaa tggtcagcaa
    12241 gtggacttag gttctcgaat cacagggtat cagcaaggtg cagcaggttc aagttttctt
    12301 gaatggcaag aaggaaactg gcgtattcga atccgtggga ataatataga aggccaagat
    12361 cccttactat tagctaaaga aatcgtagct tatttggaag aaaacagtct accagcacct
    12421 gaacaatttg gtaaaattac tgttgatatg ggtgatacga cgaatcgagc cgtagaagta
    12481 agctggcaag aaccgaaaaa tgcctatacg atcactcatc aagatcctat gtctgcgttg
    12541 aagatggcag tttctatgaa gaggttgtga aatcagcgcg ctgatctgag cccatcattt
    12601 aactccaagt cacaggaagt aatcaaaaat agcacgccgc atttttgcat aattttggct
    12661 taatacctta aaagaaaact agaattgcct cttcacacat gctaaaataa aacagacaaa
    12721 aaagaaatgg ggatataagt tgactgaata cttgaatgta ggaaaaatcg taaacacaca
    12781 aggtatcaaa ggcgaagtcc gtgttatctc aacaacggat ttcccagaag aacgttacaa
    12841 aaaaggaacg gtgctaacac tattccaaga cggaaaagca cctgtggaat taacagtcaa
    12901 aagtcatcgc aaacataaaa attttgactt attgagtttt gaaggacatc cttctatcaa
    12961 tgatgtggaa aaatatcggg atggcatatt aaaagtgtca aaagacaact ctgtagatct
    13021 agctgaaaat gaattctatt accatgagat catcggtctg catgtattag aagatggaac
    13081 tgagttaggt aaagtaaaag aaatcttgtc accaggtgcc aatgatgttt gggtcgttca
    13141 acgtccgaaa aaagctgata tattgcttcc atatatcgct tctgtcgtca aagaagtcga
    13201 tttagaagct ggagtggtcc gtgtagaaat tccagaagga ctgattgacg atgaagattg
    13261 atgtattgac tttatttcca agaatgtttg aaggtcctat gggggaatcc atcatcggta
    13321 aggctgtaga taaaggatta ttagatatca atatctctaa cttccgcgat tattcagata
    13381 acaaacatca gacagtagat gattatccat acggcggtgg agcaggtatg ctgctcaaag
    13441 tccagcccat ctatgataac atccaagcca ttgaaaaagc tgcaccggat gtcaaaaaaa
    13501 gagtaattct tcttgaccca gctggtaaac gattcgatca gaaaatggca gaagaatttt
    13561 cacaagaaga acacttagta ttcatttgtg gtcattatga gggatatgat gaacgtatcc
    13621 gcacattagt gacagatgaa gtttccttag gagattatgt ccttactggg ggagaattag
    13681 gtgcgatggt catgatcgat gcaacagttc ggcttttgcc agatgtgtta gggaatcaga
    13741 cttcagctca aacggattct tattcaaccg gtttattgga acatccccaa tatactcgcc
    13801 cggcagaata taaagggatg aaagtaccag aagtattgac aaatggaaat cataaattga
    13861 ttgaagaatg gcaattaaaa gaatcactaa gaaggaccta tcaacgtcgg cctgatcttt
    13921 tggaaacact tgaatggaca cctcaaatga cgaaattttt agaagaaatc aagaaagaag
    13981 aacaagaaaa aacagcagat gaagccaagt gactttttct aaaatcttct ttacagtagg
    14041 ttgataatat gttagtattt tatggtgagt gcaaaagcac ttaactatta cgatattccg
    14101 ctgtgagaaa agctcataag aatatttgga ataaggagaa gaaaaccatg aatccattaa
    14161 tcgaagaatt aacaaaagaa caattacgtt ctgacatccc agcgttccgc cctggtgaca
    14221 ctgtgcgtgt tcacgcaaaa gttgttgaag gtactcgtga acgtatccag ttatttgaag
    14281 gtgttgttat caaacgccgc ggtgctggaa tcagcgaaac ttatacagta cgtaaagttt
    14341 ctaacggagt gggcgttgaa cgtacattcc cattgcatac accacgtgtt gcaaaaatcg
    14401 aagtagttcg ctacggtaaa gtacgtcgtg caaaattgta ctacctacgt gcattacacg
    14461 gaaaagctgc tcgtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
    14521 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
    14581 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
    14641 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
    14701 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
    14761 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
    14821 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
    14881 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
    14941 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
    15001 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
    15061 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
    15121 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
    15181 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
    15241 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
    15301 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
    15361 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
    15421 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
    15481 cattttttaa gtgaatacaa gatcagctaa ttgaacaccg tttattcgtg atgagggaca
    15541 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
    15601 gaaaaatagt agataatccc gtcctaactc ttttattctt ccctcatagc ttccaaattt
    15661 aagcaatact tcaattaatc aagttttatc tttttttttc aataatcgta aaaaaagagt
    15721 attttttatt taaaaataaa atctttgtat taaaggacaa gactaaaata accttagaat
    15781 aaaaagagaa aaaaagcccc aaactttcta tacatcaata aaataataaa aattaaattt
    15841 agaataacgt gtaaaggatt atttgatgat tttttattga gaggagggat gaaaatgatt
    15901 aagaaaactc aaaataaaat ccaacgtata tgtcaaaaac attttgtttc tagactcaaa
    15961 gctgtatcta aaagaaaata cttacatccc attatgattt cgatcatctt gatttttact
    16021 ttcttaggga gcttatcaag tatttatgta aacgtttata cagaaacgtc aaattttgct
    16081 aaccaaacga cagaggagac aaatagtcca ccctattctg aaatacagtc catagaaagc
    16141 accaatcagt ctagcaacaa cgagacaacc tcaaccacat cctcgactca actgattgat
    16201 ccgacagaaa aagaaatcac agcagcaaaa gcagcagggg agaaagctta ttcagaaaca
    16261 ggtagaagcc agacatttag tactgttgcg gccactgcga atttcaacgg acataccata
    16321 ccagatggat atacagtgat atatgccaaa gatggagtca ttgttgcggt taacggatcg
    16381 atgagtgcgg ataaaactgc gttacaaggc gtaaccaaag caatcgcttt tattgataac
    16441 cgtccagata tccataatct gtttattggc tccacagtaa atggtactac tatcacattc
    16501 ccaaaaagta tctatatggg ggtggctacc gctggaaata cgatcgattc agttacaccc
    16561 aatgcggata aaagttattc ttttacaccc tcaacatggg gagggcttcc tgccggagga
    16621 ggtatgagta ctttttcaat cggtatacag ttagcaaatg atattccatt cacggggtac
    16681 tcgatcaaaa atgggcagat cgttgatggt aagggaaatg tttcaaattt tccaacctat
    16741 caagcagcaa cggttcaggt caatgcgaat cggacaggaa ctttttctta tacagtcaat
    16801 ggtggagcag caaaaatcgg aacgtacggg actacattat ctggaatcca tgtgggtgac
    16861 aaagtggtta tcactccaac agcagtaacg ggctattctt ttgcacaaac aaacggttct
    16921 ttcacagccc tagaaagtaa cgtcgatacg gtgacctata caggcaaaca aacaccaatc
    16981 actattaagt atcaagacta ttttggacag atgattgctc cttctaaaat aatgaatctt
    17041 acttacggaa gtgctgcaca agacctgacg actaatacgc ctactatcaa cggatatacc
    17101 tttacagctg tttcagcgac agaaacaaag aaccaatcag caatgagtgt tttagctagt
    17161 ttagatacaa atggaaatgt aatcgtaaag gacgcaaatg gaaaacagat ttctgaagtc
    17221 atcctatatt ataagacaaa tgcaacagta tcaatcaatg caaatggcag taagtactat
    17281 gatggtcttt ctgttgtacc gatcgttaaa tatgccttta atgataaaac agaatcaaca
    17341 tcccttttaa acaatgatct cagtattgat caaatcacat ggaatccaac tgatttcaaa
    17401 gcgatgaatg aaaagggagt tgaagtaact gcgcctactg aaattggaac ctatacgtct
    17461 tggcaattga caaaaagcgg actagctaaa ctagctgcca gaacaaacta tttatttact
    17521 atcgtgcaga catctgatgt ctatacgatt aagcaaatca gcggtgcggt agagctcagt
    17581 ggcagtaaga catatgatgg aaaagcaggt gtcccgtcaa tccatgtcaa gctagcagag
    17641 ggtgtcacta gttcgcttac tccagtacaa gtggcactta gcagtatgga ttacacagca
    17701 gatgcacaat cagcaaaaaa tatggttaat gctggcagct atacaattaa actaacgaat
    17761 agcggaatag acaaagtcaa agcagcaaat cccaagttgt catttactga tatagccaac
    17821 acatcaggaa cttataccat ctataaagct aacgcagtga ttactgtcga tgacgctgcc
    17881 tttaattatg atgcccaaag tcactcgatt tcggttggaa atctccacgt taccggtgtt
    17941 gtcttaggcg aatcattgga ttatacccta acagttaaca gccggacaga tattggatca
    18001 caaatagtaa cgattagttt aggcgaagga agtgtaaaca ataattatac gattatcgca
    18061 acagataccg ctaagttgac gatcaatccg actctcttgc ttccttcgac aggtgggata
    18121 ggtctactgc cctttgtctt tttaggactg atctttattt tttctggctt cttttatttt
    18181 atccatcgta agaaaaaagc aggtgaacag cgatgaaaaa aacaaaaaat gtacgtgtgg
    18241 atatatttgc tgttctcttt tttatcctac tggtcttttt tataggttct ccgactgtgt
    18301 cagctagaga atcacctatt acatttactt tgcataatcg aatgttcgaa agcgataaga
    18361 tgccaagtat cagtcaaaac gacggaataa atatcccgga taactttcaa ggagtaaaag
    18421 ggttgaacgg cgttactttc gctatatatg atgtctcgga tgaattttac aagctgcggt
    18481 cagaaggaag tagtgtggaa gacgcacaaa gaaaactagc acaaaaaagt gatagtgaaa
    18541 aaatactggc agaaaatgtg acaaaaacag tagacgagga agagggcatt gcttctttta
    18601 gtgcatcgga caaagatgag caaggtcgtg acgctgttta ccgatttgcg gaaataaaga
    18661 cttcagatta agtaaaagaa aaatcagcac catttgtcgt tgtgcttcct gtgacagatt
    18721 catctaatca taaattgacg accatccact tgtatccaaa gagcgaacaa aaaatacctg
    18781 ctgcactcac attaacaaaa acagtagaga ataagcagac agattttgca gatggggata
    18841 aagttcctta tctcatcacc gtaacaattc ctgagaacat caacgagata ggaacctata
    18901 cgatcaaaga tacagctgat cctcagttat ggctagaacc agaaacgatc gattttttga
    18961 ttggtggaga caagattcat acatttcaca cacaaaaaac aaaacatggt tttgtcttat
    19021 cagattctgg aaaagatctt agttcgtttg ctgggaaaaa attaacgatc cgttaccaaa
    19081 tgacattgaa agaaaacagt gaaaaaacga catttgataa cgatgtacgt ttgacgactg
    19141 acaatcaaac agtagaaaca catcttgcca tccaaacagg aggcaaacaa tttgtcaaag
    19201 tggatcggca aacgaaacaa aaactagcag atgctcaatt tctagtcaaa aaaaatgacc
    19261 aatacttagc gcaagaaaac gggataaatc attgggtagc caaagatgat tcaagagcaa
    19321 cgatttttac ttccagtaaa gatggaacat tttctgtcca tggattgtct tttggaagtt
    19381 acgaactagt ggagattaaa ccgcctaaag gatatatatt gagtcaatca accattctct
    19441 ttgaagttga aaaaggcagt tatgatccgc ttcatcctat accattggaa atcatcaacg
    19501 aaccaaaagc aaatccgacg aataatggaa aaacgccagt tcagatgcag acaaatggtc
    19561 catcaaatga aaaaaatgga ggaagctttc caaaaacaag cgaagagatg cttggaggtt
    19621 tctcaatttt agggttgttg ctagtccttt caacagggac agcttggttt tataaaaaac
    19681 aacaaaaagg aaacaatagg agggaaataa aatgaaaaac atcaaaacaa aaatatggac
    19741 gtttttagga actgctatca tgctgcttcc tttcgttctc ggcctaggaa ctgcagaggt
    19801 ttctaggccg tatcacctac accagaaaat gttacggtta atttgcataa attgaaattc
    19861 acttcagcac cagaaaatca aataaacaac ggaacggaat tgacatttcc gaatagcgaa
    19921 ccgttgaacg gcgttgaatt caacgtatat gacatcactg ctacgtacta tccaagtaaa
    19981 gacacagcag ttccagcaga cgctacaccc ttcgcaagtg taacgacttc tggtgaagga
    20041 ttagctaacc tgactcttcc aggtaaatct gatgggaaag atgctgtata cgtctttgta
    20101 gaaacaccaa agcccggtgt cgaaacgtcg ccaaatatcg tgttaagctt acccttttat
    20161 aacccgaatt catcagaccc tgacaaacct ttagacacga ttcacctata tgctaaaaat
    20221 gtcatcaaag aaagcaatat caaaatccag aaagctggga acttttcagg ggtagataag
    20281 ttagcagggg cgcaattcca gatcaaaaat tcagagaatc aattcgtgac cggttttgat
    20341 gaaaacactg gtgtcgctct ctatgatcct gctgagtcag cgttgacatt cacaactgat
    20401 actgaaggat ccatcagtgt caatggatta atagacggca attatacttt agttgaaaca
    20461 aaagcaccag atggttatca attaaccaat ccacaaaaag aaactacttt tatagtaaaa
    20521 aacggaaaat tgcaacaaac aaccggtatc atccatggcg cagatgaagc caatcttggg
    20581 aatccaaacg gtgtagtcaa tcgggaaaat acaactacgg ataacacgat tactgtagaa
    20641 aatgtccaaa actacggtga tttccacttc atcaaacaag atgtgaacac gcaagaaaaa
    20701 ttagccgatg cagaattttc tattctaaga gcaaaagatc caactgttaa attaatgaaa
    20761 aaaatagcaa atcttgcctc aggagaatac ccatatgctt ggtcagatga agtcacaggt
    20821 tctgactggg aagaagtgaa attgatttct gattcagaag ggaaattttc aatcgaaggg
    20881 ctgaaacaag gggcatactg ccttcaagaa acaaaagcac cagaaggcta cgcattacct
    20941 aagaatgaag ctgcgtacac tgaatttaca gtagatgatg atccaacaac ttctgatgaa
    21001 acaacgatcg ataaccaacc taaaggaatc ttgccttcaa ctggtggtat ggggatcatc
    21061 gtattcatct tggtaggtac tgctttagtt ggaggagcag tgatctactt caaaaaacgc
    21121 catcaagaaa cagaagctta aactattgga aacaaaaatc atacgataaa taaacgatag
    21181 tgattcaaat ggggagaaag gttgtgacaa gtggtcggtc tcttggtatt cgctcaacaa
    21241 acggcaaaat aggaggaact attttgccgt ttgttgagcg aatactcagg tcaaggcgct
    21301 gatttcacaa cctcttcata tacatattag aaaataggtg gacatgatga ataaaagcag
    21361 aataagaata ctggacatct tcatggcaat cattttagtc gttgggatcg ggatcttttc
    21421 ttatcctttt gtagaagatt cactgaatga ttttcttgcc cagcaaatga tcatccatta
    21481 tcaaaagcaa gcatcgaata agaattcaga ggaaataaaa aaacagcagg aaaaaatgac
    21541 aaagaaaaac caacagttgg cagagcaaaa cgtatcacca gggataggca gtttcaacca
    21601 agcagttgat gcaaaagcat taaaagattt acctagtaat gcctttttta tggaacatat
    21661 gcttggagtc atcgaaatcc caaaaatcaa tgtcagctta cctatattcg atcagacaac
    21721 ggaaatattt ttgcaaaaag gaacgtcatt attggaagaa agttcttatc cgactggcgg
    21781 gaaaaacacc catgccgttc tttctgggca tcgtggactc ccagaagcaa aattatttac
    21841 agatttgcct aagttgaaaa aaggagacca gtttttcatc cagatcaatg gaaagacatt
    21901 ggcgtatcaa gtagaaaaaa tccaagtggt cttacctgat gaagtagatt ctttgggtat
    21961 ccaaaaaggc agagatctcg tgactttgct tacctgtact ccttatatgg tcaatacaca
    22021 tcgactgctt gtgacggggc atcgtattcc ttatcaagca aaagaggcaa aaaaagcgat
    22081 ccaaggaatt gatcaatgga aaaaatggaa attttttatt tggtttatcg gtatactact
    22141 tggatcaatc ggcttagttt ggctactcat cgcttattta gattctttgg ccatcgctaa
    22201 gcgaaactat cctttatctt tttatgtaaa gaacacaaac ggacgaccaa tcgaaggaat
    22261 ggtcttttct gtaaaaacgc tcaacggcaa acactatatt actagagaaa aagttccatt
    22321 tgtgaaagct tctgatgaat acggactagt gagattttct gatttaaaag gcaggaacta
    22381 tcgcttgcaa catgaggagc tcctgcttaa aatccatgta aaacataaac attcgaaaca
    22441 gttttcaatg aaattgaaaa aaggaagata taaactgcga aaagaaaaag aggtgtacta
    22501 tttgattgag aaagaatgaa tgaagatgtc caaaaaagca gagggaacag actaaatgaa
    22561 aaaagagaga agctgacgaa atggacgtgt aaactgctat ttttggttta ctatctggaa
    22621 cgccagcttc tattttttta tgtttcagaa gaattttatt attatagtat aacttttgaa
    22681 ttagtttcca taacatttgg agcaaggggt taaaccacga gcttgagctt cgcttaaagt
    22741 ggcaggggta tacgtaccat ttccacattt atgtgtatgg tattttgaac cggtcggtgt
    22801 gacatagaca gtttgttcat tattttgctc aggagctgcg gcttgtgcct gctgagaggc
    22861 ctgttcagct gctgcttgct gtcgcgcctg ttctgcttgt tgctgtgcga ttgcttcttg
    22921 ttgcgcttgt tctgcttgtt gtcgtgcaat ttcttcttgc tttttccgtt cttcttctgc
    22981 ttcttttgct ttgattgtgg aatcaattgc agcaagacgg cttgtcaact cttgtttgtt
    23041 ttcaggtagc gcttggatag ctgctgaagc agttgcatag ttttcttttg taggatttgc
    23101 ttcagcttgt gacaaagcag attccgcttc tgtcactttt ttttctagtg cagctttttc
    23161 cgctttttgt ttctcttctt cttttttctt ttcaatttgt tcttgttctt tcgatgaatc
    23221 tgccgtactt tcggagatcg tggtactgct gctggaagcc tgagctgaac ttgtgctttg
    23281 agtagtagac gtagtagcac cacacccact caacaaactt cctgcgacca aaaacaatgc
    23341 aactaatttt ttcactaaat ttcctcctta agtaaaaaac aaccgttttc aataaatgtc
    23401 cttttcaatt gtttacttac ttgttataat aattgtgtta tgaatagtga ttgaggaaac
    23461 ttaatctaat cccgttttga tacacgggat tttttgctat agttctgtta ttaagtatgc
    23521 aataataaaa tatgtacgtt attttattat tagattattg acatttatat aataaacgat
    23581 taaatgattc atatcaatag aatacttagg aaaaaaagag aaataataac aggtttatgc
    23641 actattgacg gtgtgacaat atgatatttg tatccgtttt ttttcttgta tgtagtatag
    23701 taaagtagat acaggaggag aaaatatgca gactttttta acaaaagttt ctcaaaagaa
    23761 aattaaaatg attcatttac tgcttgaaac agaacgttgg tgttcaatag aagaactaca
    23821 aaacgaattg aaggtatcaa gtaagtctat cttgaattat ctaacagatt tagaagaatt
    23881 gttccaaaaa tatcctgata aggtcatttt gaagaatgag aataatcggc gcttttctat
    23941 ggaaaagcag gagaacttcc caatctacat tatttatctt catttctaca gaaaatcata
    24001 taattatcat ttgattgaat tcatgtatca acatcctgag aaaaatttgg aagattatgc
    24061 agacgctcaa tatacgagtg tatccacagt ttttcgttat gctaagttat tagtaaagta
    24121 ctttgaaatg acggacgtca aagaatagta gccgccagat agaaggaaat tagcagttga
    24181 aaaagctgcc ctgcatcaag taatacgacc agccaaacgg aaacagtgtc tcatgttttt
    24241 gatttggctg gtcttattac cgcagatgca agcttttgcc acacctcttt cttgtcattt
    24301 acagactcaa ctatcaatgc tgcaagcttt cccttaatgc tgtaacacca gtaagagttg
    24361 tcaagaccag cagactgact aggtaaatac cgctcaaaaa caagattacg gcagttaaac
    24421 tataatgatc catcaagaaa ccgatgacga tagaagaaaa accaccaaca gctcgtccaa
    24481 ggttcataat gaaattattc gccgtcgcgc ggatttctgt tggatacaaa ctactaatca
    24541 ctgcgccata tccaccatac atgccattga taaagtagcc gactatcaca gcggctgcta
    24601 ccaaactcca ctggctatgg gctaacacaa gtagatagac agataaggat gaacaaatta
    24661 aaaacaaaga aaaggctagt ttaggcccca atttatccat gattatgcca aaagtcatca
    24721 tccccaaaga catgcctaaa atagtactga ccatccacaa tgaagaccca gaaaccgaaa
    24781 gccctaatcg attttgcata attgaaggca gccaattcat taacccaaaa tagccggcaa
    24841 tttggacagt taccatgata ctaaggccac atgtttgcca agcaagatgt tttgattcaa
    24901 acagtctgct aagtttggtt ttcttttcgg cagattgtac agatttgaat tcctttggtt
    24961 cagtcaagcc gcgacgaatg aacaagacca tcaacaccgg cagtacacct acgacataca
    25021 acatcttcca accaaacgtt ggaatgatca cagcagctaa aatcgcagcc aaagccgcac
    25081 cgacctgcgc accaatacca gcaaccgaag aagctcgtcc aatttgtttt tttgaaaaac
    25141 tttcggaaat caaggacata cacgccccgt attctcctcc cgctcctaca cctgcgataa
    25201 agcgaaagag ataaaccaag tagatgtttg aagcaaaata catcaataaa gaagcaatcg
    25261 aaaacagaag gaccgtttga gaaaatactt tcacacgtcc atatttatca gctatgatgc
    25321 caaaaaaaat acctccaatg agcatcccca aattagtgat cgttgaaatg aaacccgctt
    25381 gcgtaccact aatatgaaaa gaagtaatca ttgaagataa tgaaaaagct aaaaacataa
    25441 tatccatatt ctctaaagca attcccgccg atgtcgataa taataacttt cgctgatacg
    25501 ttgtcatttt tatacccctt ttcctaaaat ttccctaaat tgttaaaagt atatccaaaa
    25561 aagaataaaa aaacaagact tttttatttg aaataataca caaacaaccg atatagataa
    25621 tccagcttag ttttaataga aatgagtatt gacttggagt acacttaaag ttgtagaata
    25681 aataaaatac ataagtaaag tggcaagaaa ttaagagaaa gagtgatcag atgaatatca
    25741 aacaagtcag tgaagaaaag ggcatttcgg ccgatacatt gcgctattat gaacgaatcg
    25801 ggttgattcc accagttaat cggacaaaag gcggaatccg tgattataca gaagaagacc
    25861 taaaatgggt cgactttacg atctgtatgc gaaatgccgg attatcgatc gagtctttga
    25921 ttgaatatat tcgcctatct agcgaaggag aagcaactgt atttgagcgg cgtaagcttt
    25981 tgattgaaga aagtgaactt ttgacgaaaa aaattgctga aatgcaagaa tgtttggaac
    26041 gtttacaagg aaaaatcaag aaatatgatc gtgtgttatc tagaaataaa atggaatgta
    26101 tcaagtaaaa aaatcaagga gcatcgctat ggagttacgc ttactaaaat atttttggac
    26161 aattgcagaa gaaggaacaa tctctcaagc cgcagaagtt ctacatctta cacaaccaac
    26221 cttaagccga caactgaagg agttagaaga ggaactagga acagaactgt ttgtacgcga
    26281 aaaccgcaaa atgatcttga cagaagcagg ttattttcta aaaaagccga gcagaagaaa
    26341 ttttagattt aacgaatcaa accactcaag aattcgagaa cagaaaaaaa caattattca
    26401 gcggccgatt tacgatcggc tgtgtggaag ctgataattc gaatacattg gcactattac
    26461 tagaaaagtt gatccgtgat tatcccagcg ttcaatttca tatccacagc ggaacaagtg
    26521 atgatatcac ggagtgacta gaaaaaagcc tgttagattt agcgattttg ctagatccag
    26581 tagatacaga gaaatacgaa acgattttgt taccaagaaa ggaaaaatgg ggattgctca
    26641 ttttagaaaa ttctgtttta gcaaaaaaag acgtgataac aaaagaagac tttgcgaatg
    26701 tacctctttt gatctcaaag cgtccagaaa tacagcgaat gtttgctgac tggttaggca
    26761 taaaaaagga gcagttgctg atcacaggaa cgtacaattt gattttcaat gtatttgcgc
    26821 taattgagaa tcaggtaggt gctgctttag tcatagaagg tgctgtaagt catctttcaa
    26881 agcgagtgac attcgttccg cttgacccaa tgattgaaac gaactgcatg ctagtctgga
    26941 aaaagaatcg gattttatca cctgtagtca atgaattgat tcaccgtttc aaagaagctt
    27001 ttgaacagta ataagtatct ctaatcgaag aaatttcaat acaaaagcga gacgtatgac
    27061 agtcttgctt ttgtattttt tcatttttaa atcaaaaaaa ataaagagat ttacataact
    27121 tgttaaataa gcgcttacaa acataagaag aacggaaatg aatcagctat aataaccgtt
    27181 gttctggatt gtaatcatta caaagaaatg aagattataa ctttggaggt gttcgaaatg
    27241 aaagaaaaaa agcaaaaatt cgttcaaaaa acatgccctt tattcaatgg ttgggtatga
    27301 ttctgctgat tgtattagct ttattgacga tcattccttt tttatggatg gtttctgctt
    27361 cattcaaagc aaataatgaa gtgttcacga ttccgattca atggatccct aaaacttggc
    27421 atttagagaa ttatctggtt atttggtctc gtattccttt agtgacattt tttaagaata
    27481 cagctttttt aagcgttgtg atcacgttga ttcagttgtt tacttctagt tttgctgctt
    27541 acgggttttc taaactaaat tttaaagggc gcgatatttt atttttagca tatatcggca
    27601 cgattgctgt cccttggcag tcttatatga tcccgcagtt cattatgatg agaaaagtgg
    27661 gtttaacata tacgttgtgg tcgttggtgc tattacaagc gttcaatgct tttggtgtgt
    27721 tcttgttgaa gcaatactac agtagtatcc cagattcttt atgtgagagt gcgcggatcg
    27781 atggcttatc agagtgggga atctatcgca agatcatttt acctcttaca aaaccggcga
    27841 ttgccagttt gacgatcatc acattcgtta atacatggaa cgattatatg gggccattca
    27901 tttatttgtc ttctacggaa aataaaacaa tccagttagg gttgaaaatg tttattgggc
    27961 tatttgatgc agagtacgca ttaatcatgg ctgcctcagt tgtttctgtt cttcctgttg
    28021 cttttatatt tttagcgatg caaaaatatt ttgtagaagg gatcgcttca tcaggaatga
    28081 aggaatagga ggtgattatg aaagagcagc gttttgacaa aaatgtttat atggaattct
    28141 tttattggtt gtatcaatca ttggcaagta gcctttgttt ttggtgtgtc aatatcttgt
    28201 atatagcagc tgcgttcctt ttgtcgatag atggaaggaa tgtcttttcc tttatttttt
    28261 ctttactttt tattggtcca ggttgcatcg tgatgatagg tatgacagaa gaattagcaa
    28321 aagaaaaaag tttttcacca gtacgtttgt tcataaagac gtttcgccaa ttttggctaa
    28381 agggattttt ttattggcta tttgcttgga tagttagtgt gataatgatt tttgattgtt
    28441 tcttttttat acgtttttct tatggaaaat ggttgattcc gttgtttgtt ttacttgctt
    28501 gtttatcagt ttcctttagc atcaactact ggtattttca ggtgagaaat ccagcgtcga
    28561 aaccaaatca agttctacgg atagcgttct actatacgtt gaaaaaatgg tatgtgagtt
    28621 tactagattt tttactattg acaagtttgt ttctcttctt tttcgtaaag ccccaatggt
    28681 gtattctact aggtccttct atcgtatttg gtttgattta cttcaacaat cgtaaattga
    28741 tgagaactat ggatctgtag actttataaa ttgaaacttt caattaagaa tgacagtttt
    28801 tatgtaaaaa ctgtcattct ttttcatttt ttgaaacata tggctaaaca aatgaaataa
    28861 aacgtgctat acttttgaat gaaacaggtt tagaaactgt atcaattatt tgtgattatt
    28921 tctaactaat aattaaaaat aaagcataaa aaggacgatg ttcaatggac gaaaaaatga
    28981 aacaaggttc gaaatctgta gtagaaagtt tgattaatca tcatgttgat tatgtattcg
    29041 gtataccagg tgcaaaaatc gatggtgtat ttaatgaatt agaggatcaa ggtcctgaat
    29101 taatcgtgac tcgtcacgaa caaaatgctg catttatggc acaagcaatc ggacgtatta
    29161 caggagagcc aggagtagtt atcgcaacaa gtggtcctgg tgctagtaac ttagctacag
    29221 gtttggtaac agcgacagca gaaggtgacc cagttttagc aattgccgga caagtaaaac
    29281 gaagcgatct attgaagttg acccatcaaa gcatggataa tgcagcactg ttccagccaa
    29341 tcacaaaata cagtgcagaa atccaagatc cagaaacaat ttcagaagtg attgccaatg
    29401 cttatcgtat ggctaagagt tcaaaaaaag gcgcgagctt tatcagtatc ccgcaagatg
    29461 tagtggatgc acctgtccaa gggaatgtca tcaagccatt gagcgatcct aaattaggtt
    29521 ctgcttctgc agatgatatc cgttacctag ctgaacgcat tagagaagca aaactaccgg
    29581 tactattagt aggtatgcga ggatcaagtg aaaaagaaac gttagccatc aggcaattag
    29641 ttggaaagac agcattgcct gttgtagaga cattccaagc agctggagtg atctcacgtg
    29701 aattagaagc gcatttcttt ggtcgtgtgg gtttatttag aaaccaacca ggagatatgc
    29761 ttttgaaacg aagtgatcta gttattgcta ttggctatga tccaatcgaa tatgaagcaa
    29821 gaaactggaa tgcggaaaaa gatgcgcgga tcattgtgat cgatgaagcg ccagctgaaa
    29881 tcgatccttt tatgcagcct gaaagagaat tgatcggtga tatctcagcc actcttgatt
    29941 tattgacagg ttcattagag cctcaacaag tgtcagaaga tgcaaaagaa tatttagcat
    30001 ctcttcaagc agaattaacg gagcgggaca ttgttcaatc aaaaggagaa gcaggtatct
    30061 tgcatccttt agaagttatt aacacgcttc agtcaaaagt aacagatgat atgactgtta
    30121 ccgtagatgt aggtagtcat tatatttgga tggcacgcca ctttagaagt tatgaacctc
    30181 gtcatttgtt attcagtaat ggtatgcaaa cgcttggtgt tgcattgcca tgggcgattt
    30241 ctgctgcatt ggtgcgtcca aatacacaaa tcgtatcagt ttccggcgat gggggatttt
    30301 tattctctgc ccaggattta gaaacggcgg tacgtaaaaa attgaatatc gttcatttga
    30361 tttggaatga tggacactac aatatggtag agttccaaga aaaaatgaaa tatcagcggg
    30421 cgtcaggtgt agactttggt ccagttgatt ttgtgaaata tgcagaagct tttggcgcta
    30481 aaggaatccg agctacaagt gtagaggaat tagaaaaagc tttagaagaa ggatttgcga
    30541 cagaaggacc cgtaattatt gatattccga ttgattaccg tgataatgag aaattgggag
    30601 aaacaatctt accagatcaa ttctattaag gaagtgtcga catgactgaa aaaatcttat
    30661 accagcacgg gacattagga gctttgatgg ctggcttgat ggatggaaca gaaacgattg
    30721 ctcatatatt agaaaaagga actctgggat tgggaacatt gcatgggctt gatggtgaag
    30781 tcatcttttt agatggcgta gcttatcaag gaagatcaga tggctcagtt gcccaattag
    30841 atggaagtga acttaccccc tatgcagcaa ttacagattt tacgccagat actagttttt
    30901 ctgtctcaaa aactgcagat ggagaacaat tgaaaaaaga catattagta agagaagccg
    30961 gggaaaacct atttttggca gtgaagataa ctggattatt caaaaatatg cacatccgta
    31021 tcatgccgaa gcaagaaaaa ccttacagaa gactagcaaa aatttcagag tctcaaccag
    31081 aattccaaca aagcaatgtc caaggaacat tagtcgggtt ctttacgcct gaattattcc
    31141 aaggagttgc tgcagcggga tttcacctgc attttatcga tgatacacat acatttggcg
    31201 gacatgtaat ggattttgaa gtggctgaag gaaaagttga gattagccgc atctcgtccc
    31261 ttgtacagca ttttccagtt gatgatccga cttttgttca cgaaacgatt gattatgctg
    31321 atttagcagc agaaatccaa caagcagaat agaaaaagac agcgagatgc gttcaaggtg
    31381 attaacctga cgtatctcgt ttttttgagg agaaaaccta gcgtttacaa aaaaactgag
    31441 ctataatctt ctccatagat caaaaaggag cggtttttat catgtggaca gaaaaacaga
    31501 aaaaagatgc atacgaaatt ttattattac agcaacaagg attattagaa gcagaagata
    31561 actggttagc aaatttagca aattcatctg cattgttgaa tgagactttg cctgagactg
    31621 tctttgcagg atattattta ttcgatggta atgaattgat tttaggaccg ttccaagggc
    31681 gagtttcttg cacacgtatc aagatgggaa aaggagtttg tggagaatca gctgaaaaac
    31741 aagaaacatt gatcgttgat gatgtcaaaa cccatgcaaa ttacatttcc tgcgattcag
    31801 ccgcacaatc agaaatcgta gtgccaatgg taaaagaagg taatttaatc ggtgttttag
    31861 atatcgattg cggagtaacg aaagggtatg atgaaatcga tcaagcattt ctagaaaaat
    31921 atgtagatct tttattagca aattttaata taaatgcttt ttcatttatt aataactaaa
    31981 atcgtctatt gaaacaaaaa agaagagaaa tcaagaataa aaagagccgg aaagacagtg
    32041 ggcatttctg tttttcggtt tttttatttt tttataaaat cacaagtgtt attgattttc
    32101 ataatcatat tttaatgtta ttatcatttt aaataatgaa aaaacattaa ggatggatga
    32161 aatatgaata acgaaactat cattttagga aaaaaatata tttgccaacc tattggcttg
    32221 aaacaaaaag tgattggaga agtaatcagt aaattagaaa attgtgttgt gatctgcgta
    32281 gacgaatatc atgacgtcga tcgcaatgaa atattagaaa aatgcggaaa agtagtggca
    32341 aaatatactc acgtttatga gttggaaaca acagaatatc tatataacta tagcaacaaa
    32401 tatgaacata ctttagtttc ataaagattt aaataaagtg ccttcaaaag cgagaagacc
    32461 aagcttttga aggcacttta tttagttggc agtgattgtt catgattttt gatttattca
    32521 aaaggttcgc ctagagaatc tgtctctgta acgaggcctg gtggagaaac tcgataaaaa
    32581 cctatactgc cagaactgtc ctattttcgg atagacaaac tagtagtttt aaaaagatca
    32641 tccgatccaa tcatttcaaa aaatgcaaaa ctgatatcag gatcatttag ctcaggcttg
    32701 ttatcgtaaa gcatttgtaa aggttgcatt tagttaggag taaacgcgaa actatcatca
    32761 ttatttgctg aagaagatga agaagctaat tcagagtttg tttgaaataa aggagacgtg
    32821 ctgacagttt ctttaccctg ttttttgcag aggtagaaac ttttatatcg gaagtttcta
    32881 ccggagaatt tttctcattt gtagaaattg ttgaatcgat actattcgaa atttgcgtat
    32941 ctgaaggtat gttctttgat gcagaagtcc cacaagcagt aagtaacaat aaatttgata
    33001 aactgataaa cagaattctt ttcattatgt acacactcct tatagtatcg tttagcatac
    33061 acctatgctc agtattaggc aaaatcaatg caaaactatc aagtctttta tagtatctat
    33121 tagtcttagt aatacatata tggagagggt gatatcccag ttcaaaccag atacattggt
    33181 taccatctgt attaacgatt atgcctctaa acgctttttt tatgaaaaaa tttataagtg
    33241 aaaacgtagg tatgatatta tctagaggta atttttttgg gtatttaaat agttgctagg
    33301 aggatagctt ttggattttt acagtaattt tatcttaata atagcaatat tattacttct
    33361 aaatatttgg ttttttgata agagtagaaa tgcagggata ggttttagaa ccaaacgaag
    33421 tacgtcttct gaaaaaaagt gggtatattc acagactatt ttttacggag gggttatttc
    33481 aatcagttta ctttcttcaa cactttattc ttttaatgtt atagacgttt ccatgtcaaa
    33541 ttttatatct attattggaa tccttatttc tgcaattatt acacaactac ttcttgtgtt
    33601 tgaagagaag tcaaaaaata attaaagcaa ttatgccttt tagtacagga attattactt
    33661 cttacaaatg tatagcgaat gtgaagcctg ctgttttaat tgttctagaa aaacttttga
    33721 gtgaacaatt attttatgtt aggatgaatt aatttctcta gagtttata
//
LOCUS       DACDRN010000023        33169 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDRN010000023 DACDRN010000000
VERSION     DACDRN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.023"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(194..574)
                     /gene="yajC"
                     /locus_tag="IWJ80_001341"
     CDS             complement(194..574)
                     /gene="yajC"
                     /locus_tag="IWJ80_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP9461082.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(671..1816)
                     /gene="tgt"
                     /locus_tag="IWJ80_001342"
     CDS             complement(671..1816)
                     /gene="tgt"
                     /locus_tag="IWJ80_001342"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP9461083.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            2034..2732
                     /locus_tag="IWJ80_001343"
     CDS             2034..2732
                     /locus_tag="IWJ80_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9461084.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2900..5539)
                     /locus_tag="IWJ80_001344"
     CDS             complement(2900..5539)
                     /locus_tag="IWJ80_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9461085.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6158..7189)
                     /gene="queA"
                     /locus_tag="IWJ80_001345"
     CDS             complement(6158..7189)
                     /gene="queA"
                     /locus_tag="IWJ80_001345"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP9461086.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7382..8557
                     /locus_tag="IWJ80_001346"
     CDS             7382..8557
                     /locus_tag="IWJ80_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9461087.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8561..9202
                     /locus_tag="IWJ80_001347"
     CDS             8561..9202
                     /locus_tag="IWJ80_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9461088.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9199..10116
                     /locus_tag="IWJ80_001348"
     CDS             9199..10116
                     /locus_tag="IWJ80_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9461089.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10113..10784
                     /locus_tag="IWJ80_001349"
     CDS             10113..10784
                     /locus_tag="IWJ80_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9461090.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11325..11813)
                     /locus_tag="IWJ80_001350"
     CDS             complement(11325..11813)
                     /locus_tag="IWJ80_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP9461091.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(12014..12280)
                     /locus_tag="IWJ80_001351"
     CDS             complement(12014..12280)
                     /locus_tag="IWJ80_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9461092.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12280..12543)
                     /locus_tag="IWJ80_001352"
     CDS             complement(12280..12543)
                     /locus_tag="IWJ80_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9461093.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(12536..12808)
                     /locus_tag="IWJ80_001353"
     CDS             complement(12536..12808)
                     /locus_tag="IWJ80_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461094.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            13019..13204
                     /locus_tag="IWJ80_001354"
     CDS             13019..13204
                     /locus_tag="IWJ80_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9461095.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13346..15169)
                     /locus_tag="IWJ80_001355"
     CDS             complement(13346..15169)
                     /locus_tag="IWJ80_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP9461096.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15767..18472)
                     /locus_tag="IWJ80_001356"
     CDS             complement(15767..18472)
                     /locus_tag="IWJ80_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP9461097.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18778..19725)
                     /locus_tag="IWJ80_001357"
     CDS             complement(18778..19725)
                     /locus_tag="IWJ80_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP9461098.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19915..20184
                     /gene="rpsN"
                     /locus_tag="IWJ80_001358"
     CDS             19915..20184
                     /gene="rpsN"
                     /locus_tag="IWJ80_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9461099.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20360..20722)
                     /locus_tag="IWJ80_001359"
     CDS             complement(20360..20722)
                     /locus_tag="IWJ80_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9461100.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20733..21854)
                     /locus_tag="IWJ80_001360"
     CDS             complement(20733..21854)
                     /locus_tag="IWJ80_001360"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP9461101.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21870..22220)
                     /locus_tag="IWJ80_001361"
     CDS             complement(21870..22220)
                     /locus_tag="IWJ80_001361"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP9461102.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22350..23861)
                     /locus_tag="IWJ80_001362"
     CDS             complement(22350..23861)
                     /locus_tag="IWJ80_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9461103.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24183..25535)
                     /locus_tag="IWJ80_001363"
     CDS             complement(24183..25535)
                     /locus_tag="IWJ80_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP9461104.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25688..26764)
                     /locus_tag="IWJ80_001364"
     CDS             complement(25688..26764)
                     /locus_tag="IWJ80_001364"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP9461105.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26960..28705
                     /locus_tag="IWJ80_001365"
     CDS             26960..28705
                     /locus_tag="IWJ80_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP9461106.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28985..29422)
                     /locus_tag="IWJ80_001366"
     CDS             complement(28985..29422)
                     /locus_tag="IWJ80_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP9461107.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29406..30779)
                     /locus_tag="IWJ80_001367"
     CDS             complement(29406..30779)
                     /locus_tag="IWJ80_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461108.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31101..31589)
                     /locus_tag="IWJ80_001368"
     CDS             complement(31101..31589)
                     /locus_tag="IWJ80_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9461109.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31611..32213)
                     /locus_tag="IWJ80_001369"
     CDS             complement(31611..32213)
                     /locus_tag="IWJ80_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9461110.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            complement(32282..>33169)
                     /locus_tag="IWJ80_001370"
     CDS             complement(32282..>33169)
                     /locus_tag="IWJ80_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP9461111.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT         9482 a         7035 c         6069 g        10583 t
ORIGIN      
        1 taaatataaa attgctttat ttacaggtgt ttgtatagaa aaatatataa agttatttat
       61 tattatttat atgacacacc cgattattca atattgagca atatatccca tgctcttatc
      121 tctttctttt ttgaataaaa aatgaggtag gacatctttc tgccctacct cgtctttaaa
      181 taagctttga tacttatttt tcttccttct cttctgtctc ttccagcgtt tctgttgttt
      241 cagtttttac ttcaactgtc tcagctggtt cttctgttgt ttcaacagta gtcgttgcat
      301 catttgttac gacagtccct ggtttgaccg ttttgattgc tgcacgatcg tactcaagga
      361 agatcccttc gcagtctaaa acaactgtcc gtttgtcatt gtcgatttct gaaacaactc
      421 catgtaatcc gccgatcgtt acgactgaat cacctggttt catgctatct aacaagtttt
      481 gacgttcttg ctgttgtttc ttttgcgaac gtgacataaa aaaccacatt gctaatagcg
      541 cgataaacat cacaagcatt ggtaatgttc ccattttttt cacctcacta attctgtatc
      601 tacaaaaaac actttagcag aaaaatgctg ataacactag ttttttactt aatttttaaa
      661 agattttcct ttagaagctt tttgcgtttt ctttattgaa cccgtattct tcaaaaaagg
      721 cttctctaaa ttcaagcaaa ttatcatcca tgattgcttg acgtacttgt ttcattagat
      781 tcaacaagaa gtataaattg tggtaagaag tcaaacgaat cccaaatgtt tcatcacatt
      841 tgatcaaatg gcggatatag gcacgtgtat agtttttaca tgtataacaa tcacatttct
      901 catcaattgg acggaaatca tgggcatatt tcgcattttt cactactaga cggccttgag
      961 aagtcataca tgtgccattt cgagcgattc gagtaggcaa gacacagtca aacatgtcta
     1021 ctccacggat aacaccatcg atcaatgaat cagcagcacc gacacccatc aagtaacgtg
     1081 gtttgttttc ggggatcaac ggtgtagtga attctaatac acgattcatt tctgatttag
     1141 gttccccgac agataacccg ccgattgagt aaccagggaa atccatcccg actagatctt
     1201 tcgcgctttg acgacgaaga tcttcaaacc ctgctccttg gatgattcca aataatcctt
     1261 gacggtctgg attagcatga gctttcaatc ctcgttctgc ccatcgagac gtacgttcta
     1321 ctgatttttt cacgtagtcg taactttcat cgaatggtgg acattcgtca aaactcatca
     1381 tgatatcaga gcccaattta ttttggatgt tgattgcttt ttctggtgat aaaaacatac
     1441 gcgaaccgtt caagtgattt ttgaaatgca cgccttcttc ttcgatattc cgcatatcac
     1501 ttaatgaaaa aacttgaaag ccgcctgaat ctgttaaaat cggttgatcc cagttcataa
     1561 atttatgcag tccgccagct tcttctacta agtcttcacc agggcgtagc cataaatgat
     1621 atgtattact taaaataacg ccagcaccca tttctttcaa ctcttctggg gacattgttt
     1681 ttactgtagc taatgttcct actggcatga acattggtgt ggggaaagtt ccgtgtggcg
     1741 tgatcaattc ccctaaacgc gcgccagtat gtttttcttt cttgattaaa cgatagcgaa
     1801 tggcaggttg cgtcattcat ttccctactt tctatataaa atatcttgac aactcccacc
     1861 atcataatcg tttcaagtgc tttttgcaag ttccctcggc ttccttctac tttatttaat
     1921 attctttttt ttatcctttt tttatgaata acaaaaaaaa gaaaaacatt atgacagaaa
     1981 tattttttga tacatggtat attggtgtta ttaaagaaaa ggagggaaaa atcatgcaat
     2041 cttctgcaga attaaaacgc caagcaaaag agagtctaaa aggacgttgg ggacaagctg
     2101 ttttactaaa tctaatccct acattgattg gtattgctac tattcttctt attatgattc
     2161 ctattatctt gtttgccacc accatatatg atggatcagc gagcgtctca gaagcgacaa
     2221 cttcaggagg cggtagttca ggaggcggtg gcggaattat ttccagcatc attagcgctt
     2281 tgttcatgag cggtatttct tggacttatc ttgatatttt acgtggacaa aaagatcaga
     2341 tcgaaccatt caaagatgca tttcgtggat tcaaaggttt gtttatcggc ggagtgatca
     2401 gcttagctgt gctgatctcg atctttacaa cattatgggg tttgctatta gttattcctg
     2461 gaatcattaa aagctattct tattctcaaa gctattttat ttactacgat atcgtccaag
     2521 aaacaggaca aacacctaaa gtactggata cgatcactgc aagtcgaaaa ttgatggatg
     2581 gttataaagg acaacttttc tggttagatc tttcatttat cggctggcat atcttagcat
     2641 tagcaactgc tggtattggt tatctatggc tcagcccata catttctgca acaaaagcag
     2701 cattttatga acaattgcca aaagatattt aaaagagagt gtgacttgtg accgaggggg
     2761 tggcaggaga acaccgttta tcgagaggca gtaaaagaaa cattcagccc attagcaaaa
     2821 aggaggtgtg acaacattca tgtcacacct cctttttgta tagttccagt aaaaagaaaa
     2881 tcaacttaga aacagttaat cattcctctt cttctgcttc cggtaatcga tggaacttga
     2941 tcagttcatt caccgcgatt ggtacgatag ataaaatccc taccaagaac cagtgattca
     3001 aactgatgtg tactaggccg aatagttctt gcgtaaatgg cagcaaagca acagccgttg
     3061 taatagctaa tgccagtaac gccatttcta ccaaagcttt attggtcgct aaattgactt
     3121 tgaagatcga ttggctgctg cgtacattga acacatgcag aatcgatgaa taagctaaga
     3181 tcaagaatgc aacagtttga ccgacatgct gacttgcgtc tacccatggg ctgatagtat
     3241 cgacataact acctaaataa taaccaaaca aggttacgat ggtgaagacc cctgcattta
     3301 tggcaatttt ttgcaataaa cctcgagcaa agatcccttc atttttcgga atgggtggct
     3361 gatccatgat gccgtgttcc gctggctctc ttcctaaagc aaatcctgga agtccatcag
     3421 caactacatt gacaaacagt agttgcacag ctgtaaatgg tgctccccat cctaacagca
     3481 tcgcaatcaa aacaataaag atctccgaaa tattacagct tagcaaaaag ttgatagctt
     3541 tacgaatatt ctgataaacc gaacgtcctt gactgacagc gtctacgatc gtcgcaaagt
     3601 tgtcatccgt caaaatcata tctgcggcac tttgcgccac atctgttcct gtgatcccca
     3661 tggcacaccc aacgttactt gctttcaacg ctggtgcatc attcacacca tccccagtca
     3721 tcgctacgac tgcacccgta cgttgccaag atttgacgat ccggatcttg tcttctggcg
     3781 ttacccgagc atagacagag agagctttga cacgcgcatc taattcttcg tctgataatt
     3841 gatgcagttc tgatcctgaa agtgcttctg acggatcatt caagatacct aattctttcg
     3901 ctatggcact ggcagtcacc acatgatctc cagtgatcat gactgttttg atgccagcct
     3961 ttttcgctcg cttgattgct cctttacttt ctggacgtgg tggatcgatc attccgatca
     4021 gaccaatcaa acgtaagtct ttttccagtg cttcagaaga aatatcttgc ggttcttcgt
     4081 cataagtagc ataaccaaca gcaatcactc tcagtgcccg tttaccaaag cgatcattaa
     4141 cgatcgccgc ttgttctaca tcgcctgaac taaagcgcgg agcaagaaca tcgaatgctc
     4201 ctttggtgat tgagatgtat ttttcgccca tttggtgaac tgttgtcatc atttttcgtt
     4261 cagaatcaaa aggaagctcg tttactcgtg gatatttttc ttctaattct gcctttgtat
     4321 gataattctc ttcaacagcc cttacgatgg ctacttctgt tggattacct ttgataacaa
     4381 ggtcgtcatc ttcttgctcg accgtcacat ctgtacatag tgcagccatt ttcaatattt
     4441 ccatcgcgct gttcgtcatt gaatcttcga tattcgttac ttcatcttct ttgctccata
     4501 cccggcggac gcgcatttca ttttgtgtca gcgttcctgt tttgtctgag cagataacat
     4561 tagcggttcc tagcgtttca actgctggca actgtcggat gatcgcatgt ttcctcgcca
     4621 tcttctgcac accgtatgca agtgtcaacg taacaataac agtcaaggtt tccggtacag
     4681 ctgcaacagc caaggaaatc gaagtcatga acatctcaag gagtggttct ccttgcagtt
     4741 caccaatcac aaatactaaa gccgcagccg ctaaagcgat catactgatt cgtttgccta
     4801 gctgattcaa tcggacctgt aaaggtgttt tttgcatagt attttcgttc aaaagatccg
     4861 cgatcttacc catttctgta gccatgccaa tggcagtgac gatcgctttc ccacgacctg
     4921 cagcaaccgt acatccttta aataccatat taagttggtc acctaatgac tcatcttctt
     4981 cagcaataaa tgctgcatct ttttcaactg cttcactttc tccagtcaac gccgattctt
     5041 ctacacgtaa ttgtgatgct tcgatcaaac gggcatcggc agaaatagca tcaccggact
     5101 ctaaaacaag aatatcaccc actaccagtt gctctgactc aacagtggtc actttgccgt
     5161 ctcgtatgac agttgccgtt tgtttattca tatcttgcaa ggattccagt gctttttctg
     5221 cgtttccttc ttggacaatc gccaataccg agttgatcac aacgatagcg atgatcagca
     5281 gtccttcaaa taaatcccca tgttctgtag caaatgcggt ataaaaggaa atcaccgctg
     5341 ctacaagaag gatgatcgtt gtaaaatcgg acaagcttcg aaagaatttt ttgatcatcg
     5401 attctttcgg cgcctcatcg aatttattca atccctgctt ttttcgccgt tcatccactt
     5461 cttgtgaaga caacccagta tctggatctg cttctgttac cttctttact tcttctattg
     5521 tctgcttgta ttcttccatc ctattcctcc tttaaaatct tcgtacaaaa aaaggtactt
     5581 attttactat aacatccttt ttttatttac ttcaaataaa gggcttatcg attatttgtt
     5641 aagaaagcgt aaaatttaaa gtgcttatat ttgcaaacgc taccaaagga tgctattata
     5701 tagatgtaag gaaggaggat accatcaaat agacagaaga atttctcgat agaacagtgt
     5761 aagcaaacaa agttgttaga agtacaggtt tcacccagac atgcgtgtag agtacaaacc
     5821 tataaatagt gaatgcgtat ggatgtttgg atcacgaagt atccgaaaca agaagtaatg
     5881 cgatacgtaa caattgaata acgtaaacgt ctgaaaattg aataggaaca agagatgttt
     5941 caaatgttga agatgtaacg aaaacaaaat ttttgtttaa catgaagaaa ttcaacaccg
     6001 tgctttgcat gtatgatgga aagttacaat catttctgtt tatttgaatc cttccgaggt
     6061 acaagaaagc cgagcttatc gagagctcgg ctttttcttt gtcataaaat ctttgtaaag
     6121 cttttttgtt attcaaaact agcggctttt cataacctta tttcacgaac atcgcatctc
     6181 caaaactaaa gaaacgatag cgctcttcaa tcgcatgctg gtaagcagat aaagtcaatt
     6241 ctcttcctgc aaaggcacta actaacatga ctaaagtcga ttttggcaaa tggaaattag
     6301 ttgagaaagc ttctaccact ttgaattcat agccaggcgt aatgaaaata tcagtccatc
     6361 cgctgtctgc tttgatttct ccattaaatt tcgttccaat cgtttctaat gttcgaattg
     6421 aagttgttcc gacagccacg attttcccgc catttttacg tacttggttc aatcgctcag
     6481 ccgcttcttc cgtcaaacgg tagaattcac tatgcatatg gtgttcttca attttatcta
     6541 cgctgacagg tcggaatgta cctaacccta catgtagtgt cagatagact aattcaacac
     6601 cctttgcttc gatctcatca agtaattctt tcgtaaaatg caatccagct gttggcgctg
     6661 cagcagaacc attttctttc gcatagacag tttgatagcg atctgggtct tccagacgtt
     6721 ctttgatata tggaggcaat ggcatttctc ccaatgattc aagaatctct agaaaaattc
     6781 cttcatactc aaagtcaatg atccgtccgc cgtgttccaa ttcttctttc actactgctt
     6841 gcaatctgcc atcaccaaaa gagattcttg tcccaacttt ggcacgtttc gcaggtttga
     6901 tcaatgtctc ccacgtatct ccttgtgtat tcgttagaag aaggacttct aaatgcgcac
     6961 cagtatctgg tttttctcca tataaacgag caggcagaac tcgtgtatta ttcataacta
     7021 gcgcatcacc aggctgtaac tcttcaatga tttcatggaa atgtttatct tccatctctc
     7081 cagttttccg atccaaaaca agtaaacgag aatgatcgcg gtcttttaat ggtgtttgtg
     7141 cgattaattc ttccggcaag tcaaaatcaa aatcttctgt tgttaacata tcgtcactca
     7201 ctttctatca tgtgcttcat cattctatca tgtttttcgt tctgttggca aaatcagcac
     7261 tttttcactt ctattttttg ttttcctaaa taaaaaaact accctatttg tattttttca
     7321 ttgttttagg catactagta atagatagtt cttcattgaa aatagaaaaa gaggtggcac
     7381 catgattgaa tttcaaaatg tgtcaaaaat ttataaaggt ggaaaagtcg ctgtagagga
     7441 cgtcaatctt tcatttgaaa agggtgagtt catttgtttt atcggtacga gcggaagcgg
     7501 aaaaacgacg acgatgcgga tgatcaaccg aatgactgat ccaacacaag ggaaaatttt
     7561 gattgatgga aaatccatcc aagaaattga tccagtggaa ttgcgacgtc agatcggcta
     7621 tgttatccaa agtattggat tgatgcctca catgacgatc cgtgaaaata tcactcttgt
     7681 ccaaaaattg ttgaaagtca gtcaagaaga acggaataaa acagctgaaa aaatgattga
     7741 tcttgtggaa ttgccccgtg agatgttaga tcgctaccca catgaattat ctggtggtca
     7801 gcagcagcgt attggagtcg ttcgtgccct tgcagcgaat caagatatca tcttgatgga
     7861 cgagccattc ggtgcgcttg atccaatcac tcgtgattcc ttgcaagacc ttgtcaaaga
     7921 tttacaagag cgtttgggaa aaacgatcgt atttgtaacc catgacatgg atgaagcgat
     7981 taaactagca agccgtatcg ccatcatgag tgaaggccgt gtgatccaat ttgatacacc
     8041 agaaaatatt ttacgtcatc ctgcaaacga tttcgtagaa gagttgattg gagaagatcg
     8101 cctactacaa gccaagccaa atgcgacaag agtcggcgaa gttatgctaa atacagcaat
     8161 cacgattaca ccggaaaaat cattacaaga agcaatcaaa ctgatgaggg aaaaacgtgt
     8221 agatacgttg ttagttaccg ataattcgaa tgtattaaaa ggatttatcg atgtagaaac
     8281 gttgaataaa aaacgaggaa aagtatccag tgtcggcgac attttgaata aacatgtttt
     8341 ttacgtaaaa gaatccgcct atcttcgtga taccttgcaa cgcattttaa aacgcgggct
     8401 gaaatacgtg ccggtcgttg atgaacagaa caaagtcgta ggtatcctga ctcgtgcatc
     8461 attagtggat atcgtctacg atgtgatttg gggagaagat cccgcaacta ctgatgttgc
     8521 agagtcagct actcctgaag aatctaagga ggtctgacct atgcagcagt tttttcatca
     8581 gcacggttca gaaattctct caaaaggttt tgaacatctt tatatttcag gacttgcttt
     8641 gatcattggt atcatcattg ctgtaccgct tggtgtctta ctgacacgtt ttccaaaagc
     8701 tgccacgatt gtgattggct taaccagtgc cttgcagaca atcccttctc ttgctttatt
     8761 ggcattgatg atcccgctat ttggcgtagg taaattacct gcgattatcg cactctttat
     8821 ttattcatta ttgccgattt tacgtaatac gtacattggg atgaaaaatg tcgattggaa
     8881 ttatcgggat gtggcaaaag gcatggggat gactgagttc caatccattt tttccgttga
     8941 attgcccatt gctatgccaa cgatcatggc tggtattcgt ttagctgccg tttacgtgat
     9001 tgcatgggct acacttgcct cttatatcgg cgctggtggt ttaggcgatt tgatttttag
     9061 tggactgaat aattaccaac cagatctcat tttggcaggt acgatccctg taacaatttt
     9121 agcactattg gctgattggc ttctaggtct cttggaacac cggctgacac cgatcgcact
     9181 tagagaggag aatgaagaat gaaacgtata ctaaaaatga ccatggttct tttctctctt
     9241 cttatacttg cgagttgttc cttccctggt cttgccagca atacagatga agacaccatt
     9301 tcgatcacag gtggaatcac ttcagaagca cagatcttag caagtctagt tgccggaatg
     9361 gttgaacatt atacagataa aaataccgca atcatcaaca atctggctac tacaactatc
     9421 aaccatcaag cgatgatgaa cggagatgct tcaatttcag cggctcgcta cacaggaaca
     9481 gatttaacga ctacattgaa ccttcctcct gaaaaagatc cgaaaaaagc gtttgctacc
     9541 gtgaaagatg aatttgagaa aagatacggt caaacgtggt tcccgtccta tagttttgaa
     9601 aatacgtatg tctttcttgt tcgaaaagac accgcacaaa aataccatct atctaaagtc
     9661 agcgatctga aaaatgtagc ggatgaatta gtagctggtg tagatacttc ttggatcaat
     9721 cgtaaaggag acggttatga cggcttccaa gaaacttatg gcttctcctt caattcgata
     9781 ttacctatgc agattggact tgtttatgac gcagtcgaag caggaaaaat ggatattgtt
     9841 cttggctact ctacggatgg acggatcgcc agttatgatc tagtcatgtt ggaagatgat
     9901 cgtcaattct tcccaccata tgatgctgct ccagttgtag acaacaagat attagctgat
     9961 acgcctcatt taaaagaagc cttaaataag ttaggtcata ccatcagtac ggaaaaaatg
    10021 caggaattga actacgaagc agataataat ctagtggaac catcagtagt agcacaacgt
    10081 ttcttacaag aaaaccacta ttttgaaggg aagtgaagca attggataaa atgaatgtct
    10141 ttcaacaatt tttctattat ttccaggaaa atggctccta tatctttgca caatttatcc
    10201 gtcacttcct gatttctatc tatggtgtcc tctttgcagc agttgtcggg attccggttg
    10261 ggatcatgat ttctaggaga cgtaaattag caaactgggt gattcgtttg gcgaatatca
    10321 tccagacgat cccttctcta gccatgatct ccattttaat cattggatta ggcctgggag
    10381 tcaatgtcgt gattgttact gtatttttgt attccctctt accgatcatc aagaatacgt
    10441 ataccgggat gatccaagta gacaaaaaca tccttgatgt cggcaaaggc atggggatga
    10501 ctgctagaca acgactattc atggtagagt taccactctc tgtttctgtt atcatggcag
    10561 gcataagaaa cgcgttagtt gttgccatcg ggataaccgc gatcggtgca ttcgtcggtg
    10621 ctggtggtct tggagatatc attatccgag gaaccaatgc aacagagggc acatcgatta
    10681 ttttagcagg tgcattgcca actgcattaa tggcgatcat cacggactgg cttttaggta
    10741 ttttggaaag acggctagat ccagccagcc gtacatcacg ctgatgttat tgtaaaaaac
    10801 aaaagcttct gcgaccattt gttggtcgca aaggcttttt tagttctgtc atttttaagg
    10861 caataatcca ctctgattct tccctcctta tgggcaaaga gttgcttttc ttagctattt
    10921 atccattccg tatactccaa cttcatactg ccaacgggtt catatctgca tccaccgact
    10981 aagttttttt cttatccgct aaggcatttt gccaatccat tttcccatca ctcgtcatgg
    11041 aattcatacc acatgatctc taaatactcg tctgcttgtt ctttgaattg ggcatcaatt
    11101 cttacatcct ttggcgtggc tttcatttaa gccttttcca tcagtcctcg attgttcgat
    11161 gccttcagct gcatcatcaa aactggtgac tgccgtatcg gtaacttcga tcatctttta
    11221 tacaatcggg aacttgccat cctcatcctt ggctaaaaac atatctagcc attccagctt
    11281 ctcgccatgg gaggtttgcg gttcctaaaa ccggtacacg attcttatgg actgcatccg
    11341 tcgcaactag acttggcgat acgatgatcc ttcaccagaa gagcaccccc gtaaaccaat
    11401 tgatcgatat attgccaata agaaaagaca ttggcgtaaa agatatcgat ccctcgtgat
    11461 gcattgccgt tgatactgct attcatggcc gaaagcgcca cgactttaac ttcttccgtt
    11521 tgagtgacat tcatgttcgg caatgtcgct ttggataccc gctctgctaa aggaacgaca
    11581 ctggtattgt atttcgtatc tgtatctttt ccaaaagacc atgccaacga atcctctggg
    11641 aaccaataag acgactccgg ttgattgtcc attgcttttt tcaacatact tttggctgtt
    11701 tctcgataaa cgacatttcc aattgtcttc gcctcacttt ggcgataatc gatggtcgtt
    11761 gccactgcca acactaacaa cgatgctcca attttactga ttttccttgt cacacttctt
    11821 cctccttgtt ttacataaac ccgtcaacat tccattccta aactcaaaca atgacccttc
    11881 aaaggtggag atataggaac aaattaaaga aatcatgcaa taaatccaag aaggttggtt
    11941 ttagaacaaa aaaagagtct cctttttggg gagactcttt gttaactatc taaaaaggtt
    12001 ggctcttctc acttcactca taatgtgtcc aggcagaata aatttctaca actttatttt
    12061 cctcatcaac tttgtaaacc actcgatgct gtctattcag cctgcgtgag tatctgcctt
    12121 catgtgtagg tctcagcttt tcaaaagact gcgtgggcat atatggatct tccttcaatg
    12181 tctgaatcac ttcttcaaat tgtttcttta aattcgtttg cttgattttt cttaaatcca
    12241 ctttcgcaga gtttttgatt gcgactgtat agttactcat caaagagctt cccaatcaat
    12301 atcatcgata ttcgtaaagc tactgtcgtc tttttcgcgg tctcgtactt tatctaaagt
    12361 acctgtttgc tcaagaaaaa gggtttcttg aatcgctctc cagtcttcca gcccaatcaa
    12421 tacagcattg tttccactac gatcactaat aatctcgatt tcagtatgat tttcatttac
    12481 ctctttcaat aactgataaa aatttttacg agcagtgcta ggattcatcg ttgtattagc
    12541 cattgtctct cctccttttt ctgatataat tatagcgtac ttgattaagt acgtcaaaaa
    12601 aatacaataa agcacttaaa ttcagcaaac ttttcctctt ttttacatag agaaaaagac
    12661 ccctttttag ggaggatctt tgtccacgct cacttcttac tcctttacga ctagcggtag
    12721 ccaaacgtaa agtaattggt aacaacgtat catctttagc aaaggtcgaa tccaaagcct
    12781 gtgagtctat gcaacaacac caatacacga aggttcctcg ttgcagtgtg acttgattga
    12841 tatcttgaat aacctgagga tacgcacgaa agatttgtaa cggttattca aaactgattt
    12901 tggccgtcaa gtcttctact gcctcaaaaa tgattgcctc tttcgtctcc tgtcattcat
    12961 gttcactatc gatttgatac ttgcttagac cgttgggttt gtgaatcacc aattaaaaat
    13021 ggcactgagt tatggtaaca aaaaatggtc acttgacgag gctgagattc ctacttatga
    13081 agacacatgg cacttaagag tcgaggtaaa ggaagaagct gaaaatatag gaggctgtac
    13141 attatttggc aaaggattcg gaaattatga tcaagacatc aaaatcaaaa tgttttacag
    13201 ctaaatagga agaataataa catttcatac gctgttccca tctttaatgg cttatagagg
    13261 gcacctttag ctgagaataa gacaaaacag accctctagc ttcaattaag agctagaggg
    13321 tctgtttttg attttaaaac ttggtttatt tcttcaaatg gtagctatag gtgctgacaa
    13381 cgatattttc acgccaagac aatcgcaatc ttaaccgcaa acttgtttgg ttatggagaa
    13441 tattctgcca gcgtctgttt ggtacaaatt gaggaatcaa cacagtagtt gtatagtttt
    13501 gtttcgctgc atttttactt acgatatcta cataacggat aattggattt tcaatcgagc
    13561 gataagaaga gtggacgatt gaaaaacgga catctgggaa atgcttttta aattctgctt
    13621 cgatttcttt ttctttttcg acatcttcgt ctagcgaaac atgcatcgca acgacataat
    13681 caccgatcga acgtgcgtaa ttcaacgcac cgatatttac ccgtgtcaca ttcccgacta
    13741 atacaagaac ggtattcccg tcataatcat gtagttttgc atcattttct aaacgaagct
    13801 gttctgctac ttttttataa tgatcatgga tcttataaaa aatgaacata acgattggca
    13861 taatgatgaa gaaaggccaa atgtccccta accgatagac aaacaaaatc gcaatgattg
    13921 catatgagat gaacgcaccc gtaatattgg caatcgattt gcttaaccat ctttttcctt
    13981 cttttttcca tttgacgacc atccctgttt gagataaagc aaatggaatg aacacgccga
    14041 tagagtataa cggaatcaag cgttctgttg agccatggaa aataaacagc aatacgatcg
    14101 aacctagcgc tagtgtgata atcccattgg aatagcctag tcgatcccca cgatcttgat
    14161 acatgtgagg catgaattta tcttttgcca gattgtatgc aagaactgga aaagcagaaa
    14221 aaccggtgtt agcagcaact gctaaaatca gcgctgtagc aaattgaagg acgtaataca
    14281 aaataccatg tccaaagaca gctttaccaa tttgagacaa aacagtgact tctttttcag
    14341 gaacgatccc ataccaatag ttgataaatg tgatccccac gaagaagaaa cctaagatca
    14401 atgccatcat cgtcaatgta gcagcggcat tttttgctct tggcttctta aagaatggta
    14461 cagcattact aatcgcctcc accccagtta aagaggaaga accagaagaa aatgctcgta
    14521 aaatcaaagc gatagaaaca cccggcacag ccgctcctgg aagtgcagta gcttgcaatg
    14581 gcacagcacc tgtcacgata ttatataaac caacaacgat cagtaacgag atcacaagga
    14641 taaatgtgta caccggaaat aataggaacg atgcagattc cctcagccca cgaagattca
    14701 gcatcatcag tagcaaaacg attgtcacag aaatagcgac ttgatgaccg tagagtgctg
    14761 gcaccgctga tgtgatcgct tcagcacctg ctgagacaga tactgctact gtcaacatgt
    14821 agtcgatcaa taaagagcca ccggaaatca agccagcatt ctttccaaga ttctcactgc
    14881 tgacgacata tgctcccccg ccatgaggat aagcatggat gatttgacga tatgatagcg
    14941 ttaatgaaat caataaaata ataacaaaag cggcaatggg tagcgaatac cagatggctg
    15001 cagcagacaa agcgactaaa acgacaacga tttgttccgt accatatgca atagatgata
    15061 acgcatcaga cgacaataat gccaaagcag caaatcgggt taatttgtgc tcatcatttt
    15121 cagcagattt taaaggctta cctactaaca acctttttaa gtaatccaat ttctaaactc
    15181 ctttacacac aattttactt caagttttat agtcgaaaat ttatagtaaa caagatgtta
    15241 tagtacgctc actgacctta aaagtcaata aactttaaga aaataattgg aaaaaaacag
    15301 aaaaatatca agcgcttttt caaacgcttt aatacagcaa aaatctaaca tttttttatg
    15361 ggctttttct ttgaattcta taataaaagg atagaacttg acttagaaaa tcaagttcta
    15421 tcctttttga tatcttaact tttggaagag agatgtaagg ctgctttcta cccatcattc
    15481 ttactactcg aagcatagcg cagttgtcac aacctcttga taaacggtgt ttaaaagctt
    15541 gcatctgcgg taataagccc aaccaaatta aaaacataag aagctgtttt tatttggtcg
    15601 ttcttattac ttgatgcaga gcagcttttt tacaacctct tggtacacgg tgttcaatca
    15661 gctgaccctc ggtattagct caaaaaaacg gcaaaatatg aggaactatt tttccatttt
    15721 tttctctaat actcaggtca aaacactgat ttcacaacct ctttttttaa tgaaaaatat
    15781 ggctctcatg cgccgcttct ttgaatttcg tcattttttc ttcgatgaac ggatagattg
    15841 ccgttcctac gattccaaag acaacaaaca aaccgatcat gatccagatt gctttcgtag
    15901 catttggcca ataaattcct cctgcagatt cccgtaacaa gtttaccgca tgagtaaatg
    15961 gcaagaacgg attgatcatt tggaagaatt tccctgagac ttggatcgga tagttcccgc
    16021 ctccgccaga gatcgacagt actaagatga taatagcgat ccctttaccg acattatcga
    16081 ataatgcagc caagacatag acgatcatca tgaacgctaa ggcgatcaag acagcaaatg
    16141 acacgctgta gaccggattt tggacatcta ctcctagtaa gaaaatattc cctaatgtaa
    16201 cgatcaagct ttgcgcaatc gccattgtca aaaatgtcag catacgagca acgaattgtt
    16261 ctcgtttcgt aaaattgttt cggtctttct ttcctaaata gtattctgtc gtagcaacac
    16321 ttgaaagaag aacagcgccg acccacagac atagtgcagt ataaaacggg gtgctagctg
    16381 aaccgttatt tgcgattgga taaagcgcat tggtttgtag ctcaacaggt tgtgtaaaga
    16441 agtcactttc ttttgttgcg tctagtttca ataatttgat gacttcgcct agatcgactt
    16501 cttgttcgcc ttttcggatc gcgtctgctg ctttttggat accagctttg atatttggcc
    16561 agtcattgtt gatcaagtcc gcagcagcgt tcacagctgt ttcgacttct ggcatttttt
    16621 catttactgt tgtcaatgta ccggtgattt cattttttag tgctggccaa tcattttgga
    16681 taaaatcagc ggccaatcct aatttttgct gaatgatcgg taattcattg ttgtataggt
    16741 ctgccccttc attgatgcca ttgacgatgg tatccatatt tccattcagc attgtattgg
    16801 catcatggat ttcttgtcca attgcaggca tttctttttg atatttttct aaaattgcca
    16861 ctgcattcgt tacagttgct ttcgttgagc tgagaagcga atcaaagtcg atctggtccg
    16921 ctttgctcag tgcgccttgt gcagtcccaa tcgtatcgat gacttggtct agtagagaag
    16981 tgacagtgga actgatttga cctgtattga taccagcaat gatgccattg atattattaa
    17041 caacggcatc aatttgatct agcaattcat caatagcctt cgtgtcttca ttttgaacag
    17101 ctgtgttcaa agcagatagg cggttttgca gattgcttag caagggtttt aattgattta
    17161 gctgatcaat aattggctgt aatttcgtat ttccagcact tgtttgcaat ttagtcagga
    17221 actcgaccag tttatcgatt gcattttgtt gttgagatat tccgtcaata aagccgttca
    17281 atacattgct gatgtttttc ttttcatcat cagtcagctt accatcttca atagctgttc
    17341 ttacagtatc tattaacgaa cctgtcgttg ttgccacttg tccaattgac ttcaatgtaa
    17401 catctacact gtttgtgatg cttggtatcg catctttcag ctgagaggca cctgattttg
    17461 tttggtttgc tagatcactt gcctgatttt ctaaattttt cacatctggc aagatatctt
    17521 gtacttgttg gatgatcgtc aagccttgtt ttgcttcatc gattccttgg ttcattgtct
    17581 cttggatcga agcaaaatcg ccgtcgatct ctgctagttg cttaccggca ttttggatct
    17641 ctgggatttt ttcttgcaaa tcaaggatga tttttgcttg atctttcaac tcaggcattt
    17701 tgtcattcaa tgcaacgact ttttcaccca tctcatctac tttagggatg taatccacga
    17761 attcatttgc tttattcagt ttttccttga tttcaggtag ttgactttgt aattcgagga
    17821 cttgttttgt gtagccgtcg atcgtgtcca aattttcatc tgttgataaa atcatatctt
    17881 taactttatt gatacttact aggttcgtat cgatgtcgta acctatctca ttgaatactt
    17941 tcaataaagt cgaactggct gttttgataa attcatttgt gatttgcgat tggatcgaac
    18001 ttgctccttt atcggtgatt ttcggtgcaa tcgcattgat tttttcattt accgtatatt
    18061 cgatttttgg tttcttgata tcaccactag taaaactcag cagatcttct gagaaatcct
    18121 ttggcaagta aatacctgcg taatactttc cggaacggac tccgtcttct aattgttctt
    18181 ttgaatcaac gaattgccat cctaattgtt tatttttatg aagcgattcg atgacttgct
    18241 ccccaatggc cacttcttta ccttgaaact ctgctggctt gtctgcactg taaacagcaa
    18301 ttggcagctc tccagtattt ccatatggat cccataatgc tttgatgtta aaccacgcat
    18361 atagcgaagg gatgatcatg atcgcgatga tcagaagtgt tgcaatcggg tttttgaaaa
    18421 tccgtttcca gtcaagcaaa tataatcgaa gcgtattttt gattgactgc atagttctcc
    18481 cccttcttct tttttcagct gaattacttt accatgtatt tcatactttt ttcaatcggc
    18541 ttttttcaaa aaagtcgaat ttttggacat atcacggcaa ttgtctcatt gtaatctctt
    18601 acatattgag aaataattaa ggattacagc ctttacttta tcatgaaata aaagggaaag
    18661 ataattttat gccaaagttt ttatctatta tacgtatacg caaaaaagct aaaacatttt
    18721 ttgtctttgc ccctacaaaa atgtttcagc ttttttcgtt ttttattcct gtatttctta
    18781 tttttcgaat gacaggatga ttttcccagc aggaggatgt ccttcaacta gctgatgtgc
    18841 ttggatgaca tgcttaagtg aagcagacag attcattgaa ataaaaacat gaaaggctcc
    18901 tttttggtag gctttgatta tgccagcaaa ggcttctgca tctagatatt cctttcttgg
    18961 aacaaacgat tcataaaagc cttccttttt ttgccgcaaa tctagatctg ggaggtcatt
    19021 taatgcaaca tacctgccgc ctggtttcat gatctggatg ccggtttctc ctttgatact
    19081 tcctttcgtc gcatcgatta caaggtctgc ctgatcagca aattgcagac ctggatttgt
    19141 tttatcataa gcagcaaatg ctgaagctcc taattttttt accttttcct catttttact
    19201 ggatgcactg gttaagattc ggattccctt ttcatggaga agctggatca gacttgaccc
    19261 gaccgcacct gatgctccta gtatcattac agtatctgtc ggctggattt ccgtcaaatg
    19321 gttgattaga ttataagccg taattcctgg cgtgaccatt ccggctgctt cttcccaact
    19381 cattttatct ggaatttttg ctacttttgc tgatggcaac acgacttctt ctccgtaagt
    19441 cccgccgact gcatggacag ctactcgatc acctacatga acagttgtga catctgaacc
    19501 aatctctgtc acaataccag cgccatcatt acctggtaca taagggaatt tcaatgttcg
    19561 tatctctttc atttctccta aacgcaaggc aacatcataa ggattcacac tgaatgcttt
    19621 tatttctaca cgaacatgtc cagaaccgac ttctctggga tgagcatcga tttcttcaaa
    19681 aacatcttgt gcttctccgt aaccgttaat tgcaaatctt cgcatggtca ccatctccct
    19741 ttttctttaa gtttacgttt atgcctttat tttgacaaaa aaaatgtctt ttcgttttgg
    19801 cgcatgattc aaaacaaaat agttgtcttt tttcgcgtaa cgtgttaaat tatttatcta
    19861 aaagcgtaaa gattacgatt tacgctaata aaaaataatt ggaggaattt gttgatggct
    19921 aaaaaatcaa agattgccaa agcaaaaaaa cagcaagaaa cgattgaacg ctatgctgct
    19981 ttacgccagg aattaaaagc gcaaaaagat tacgaaggct tgcgtaaact tcctttagat
    20041 gctcgtccag aacgattgaa aaatcgtgat ttaatcgatg gacgccctcg cggatacatg
    20101 cgtaaattcg ggatgtcccg tataaaattc cgtgaattag cacataaagg tttgattcca
    20161 ggagtaaaaa aagccagctg gtaatccaag tctggctgta atgtaaagga ggagggcgca
    20221 tggaccactc cttggatcat caaaaaagat acgttcgctt cttctagcag aaatgctggt
    20281 actagaagca gacgtatctt tttttacgca cagctggtcc ttttttatat gtaaaggctg
    20341 catggttact tcgctatatt tatttagtaa caggagtcag tgcttcgata cccacactaa
    20401 ttttcaatgc tcggtcaact tctgccataa tgggtcgatc caaatggcag accttttctt
    20461 tcaatcttga tttatcgatc gttctgatct gctccaacag gataacggag tctctttcta
    20521 ttcctgttcc agtagctttg atcccaatgt gagtcggaag cttaggttta gccatttttg
    20581 ccgttatggc cgcaacaatg atcgtcgggc taaaatggtt acctagatta ttttgaataa
    20641 ccaaaactgg gcgtgttccg ccttgctccg atcccacgac tggagaaaga tcagcaaaat
    20701 aaatatctcc tctttttacc ataggatcct ccttattctt gatattctct tggtatccgc
    20761 tgtcctaatc cgcaagccac ttcatagtgg atcgtaccta attgatctgc tatatcctgc
    20821 atagtgattt ctttgtctgc atttttgccg ataagcgtca cttttgttcc gacaggaaat
    20881 tcttgaggca atcggatcat cagttgatcc atacatacac gcccaatcgt ttcacagtag
    20941 ttcccttcta ccaatagaga aaaaccctgc atctttcgtg gccagccatc tgcataaccg
    21001 atcggtatcg ttccgatcca ctctgcttca ggagtgatat atgtttctcc atagccgatt
    21061 ccttcacctg ctgggagttt tttgacttga atcaattcag aaaccagctc aagtgctggc
    21121 tgcaatggat aaacttccgg tagtgcgtgc ccagagggat taagtccgta cattgctact
    21181 ccataacgaa tcatattgcc gattgtttca tcatgccata aagccgttgc actattgctg
    21241 acatgtacat atcgaggaag ttcgggaagt tttttcagca cttctataaa gcgttctttt
    21301 tgcagattcc aatatgtatc atctgcctga tctgctgtag aaaaatgggt aaataaacct
    21361 tcccattcta atacacgact ttctttgatg aatgcagctg cctcttttac ttcttcaggt
    21421 gtacaaaatc cgatacgacc catcccagtg tctgctttta gatgaatcga aagaggcgtc
    21481 tctagcttac ttttctctaa aacagcagct gctgcttgca accattcccg tgtacctgca
    21541 gttactgata aatcatgagc aacagctaca gtcagtgcgt cgtaaggaac catattcaag
    21601 ataaggatgg gctgagtaaa tccagcttct ctcaactcga ctccttcatc taaatttgaa
    21661 acacaaaagc ccgaagctcc acctgcaact gctgcctcag ctgtttcaat agctccatga
    21721 ccgtatccgt tcgcttttac tactgcaaac aattcttttc cttgcggaag tcttgcaact
    21781 tcatttgcta cattttcagt tatagctttt ttatgtatca ctgctttcgt tgggcggtgc
    21841 caagcgacaa ccatcgtttt tccttctttc tagtcttcca aaataatttg tgcaaaagct
    21901 gtttcatttg tatgagtaat actgacccat acattgcctt catgaggtga acgcgtcaca
    21961 acaggctgac cattttcatt ttttaaaatc tcgacatctt gaaaggtaac ttttccgatc
    22021 ccagttcccc aagctttaga gaaagcctct ttgcaagcgt aacgcccgcc taaaaattct
    22081 acttggcgat gaaaaggcaa ggactggaat agtttcattt catcagaggt caagattcgt
    22141 gcaatgaatt ttggtttttc ttcgatcagc cgagtgatcc taggaagttc gactgcatcg
    22201 atcccaattc cttttatcat atgcttttct cttttcaata aatttagaac actctcattc
    22261 taccaaatta taaccatttt gccacaaata agtccacaaa taacaaaaat gaaagtttct
    22321 cttaaaaaag agaaactttc attttatgtt taatcgttat ttgaacggat tacaaatccg
    22381 cgttttttat cattgtgacg tttaccgcca tctttttggc tgttattatt tttgctataa
    22441 ccgccttttg atttgtttcc acgataatta ccgccgtcac gacgattacg gttgtttccg
    22501 ccaccacgtt tgccattttt attgtatcct tttttctgca ttggcaatgg gcgttctggt
    22561 gtgatcttca ctggcactgc atcagctgga tcttttgctg tcgtttttag caaaagtgca
    22621 actaaatctt gtgcagaata ttcttctaat aatttatctg ctgtttgcag gtatttatct
    22681 agaccatttt ctgcaagttt tgtttcgatt tgttcaactg cagcacctaa ttgacctttg
    22741 aatgcttctt tttcggttgg tggacgtaaa gtcgtcatcc gttttttcgt caagttttca
    22801 atcacatgca agtagtccat ttcatttggt gtaacgaaag tcacagacat accgcctttt
    22861 ccggcacggc ctgtacgtcc gatacggtga acgtagcttt ctggatcttg agggatatca
    22921 tagttgtaca catgagtgac acctgaaata tctaatccac gtgcagcaac gtctgtcgct
    22981 acaaggatat ctaaatgacc gctcttaaat gaacgcaata cgctcattcg tttttgttga
    23041 gacaaatcgc catggattcc ttctgcacgg taaccgcgtg cttctaatcc gcgtgccaat
    23101 tcgtctacac ggcgttttgt acgtccgaag acgattgtta attcaggtgt ttgaacatca
    23161 aacaagcgag tcatgatatc gaatttttcg tattcttttg cgcggacata gtattgatcg
    23221 atcaagtcag ctgtcatttc tttcgctttg atcttcacat gatgtggatt tttcatgaat
    23281 ttaacgccaa tattcttgat agctggcggc atcgttgctg agaaaagtaa tgtttgtctt
    23341 tgatcaggaa cttgtgaaat gattttttcg atatcttcca agaagcccat attcaacatt
    23401 tcatctgctt catccaaaac taatgtttga acggttccaa gtttcaatgt gtggcggtta
    23461 atgtggtcaa gcatacgtcc tggtgtaccc acaacgatat gtggacgatc tttcaagcca
    23521 cggatctgac ggccaatatc tgctccgccg taaacggctt gtacacgtac ttttttgtcg
    23581 cgccctaaac gatataattc ttcttgtgtt tggatagcta attcacgtgt tggtgcgata
    23641 actaaacctt gtaattcgtg acgatctgga tcgatttttt ctaacattgg taatccaaat
    23701 gcagctgttt tccctgtacc tgtctgtgct tgtccaataa cgtcttttcc agctaatgct
    23761 agtggaatcg tttctgcttg gataggcgtt gcttcttcaa aaccagcgcg ttcgactgat
    23821 ttcaacaatt ctggtgataa ttctagttct ttaaatttca aatgaatcct cctatatggt
    23881 tgttaactga ggatgtagct tttttgctac cccgtcgatt tgttcggtca aagtgtattg
    23941 tttctttttg acagtggcca cgcacgtttt tatccattct tagcccctgt ggcaccaccg
    24001 aaaaatcact ttgcgaagtt taggcattag aaaagccaaa cgggtcgcgt tcagctttat
    24061 cattattatg acatctggct tcaacaagtt gtttctatgg caaaaccagt ccaatttctc
    24121 ttcatctttc ggcaataggt tattctaaca tagtttttcg ttttctgcaa gtttacagat
    24181 ttttatttca tttgctgaag ttgttctacc acttcaaata agcccatgct gttgctgcct
    24241 tttagcataa tactatctgt tgcttcaaga ttttgtttga tagcatcaat caatttattc
    24301 ttttcttttt caaaatagaa cacaggtagt tgggggtatt tttggttcaa ttcttctttt
    24361 agtgcatgca tttgttcccc ataaaggaaa atcatggcga atttctcagg atcgatatgt
    24421 tcactcattt gcgcatgcat atttaaagac tctttcccta attccagcat atctgctaac
    24481 acagcgattc tgcgtccttt tgttggcaga tttttaaaag tatctaatac cagtcccatg
    24541 gctgttggat ttgcattgta gacatcgctc aaaatagctg caccatttcc tgccgttagc
    24601 cattcggttc tgttttgagt gatagaaaca tgggcgagcc cttggcgtat ctcaggtatg
    24661 gacagtccaa agaagcgccc gatcgtatac gcaatcaaag cattcgttac attataactt
    24721 ccaggcagcg ggatcgtaaa tgtttctcct tctactacaa aatcggtttg tgcttttcct
    24781 tttgctgtca cttccgcatg aacatcgcca tttccaatac cgaatgttgt gactgtttgt
    24841 actaatgttg ccgttaaagg tgtcagcaac ggttcatctg cgggcacaac gagtaatccg
    24901 tctgatacaa gtccgtcagt gatttccatt ttggctttag cgattccttc tcgcgaccca
    24961 agattttcta tgtgtgcttc accgatcatt gtgattgctg cagcttttgg ttgagccata
    25021 agtgaaagtt ccgtgatttc attggcgtga tccatgccca tttccaaaac gatcttttct
    25081 gtatcttggg gcatgtgcaa aatcgtgtaa ggaagaccaa tattattgtt gtaatttcct
    25141 tgcgtcttat atgttttgta tttttcagac aagacagacg cggttagatc tttcgtggtg
    25201 gttttaccat tgcttcctgt aattgcaacg acttctgcgc ctactttttc caagtaatag
    25261 gttgccaatt gctgcatggc tttcaaagga tctgctacag gaataactgg aaaatttttt
    25321 ggagcttgtt caacaggcaa gctccaaaat gaagcagatg ctccgtttgt gatcgcttgc
    25381 tggataaatt catgtccatc tcgcgtacca gcaagaggaa cgaataaact tccttcggtt
    25441 atcttccggc tgtcaaattc gacattggta atctttgtca gtgcagagca ttcgctgccc
    25501 acggcttgtg caatttcttg tattgttaga ttcattgaga tctctcctaa aaaaggttgt
    25561 gaaaaaagcg tttggcctaa acggcgggtt cgcatgactc tcacttccaa gtaaatttct
    25621 ctattcgtat ttagaagcta gaatgaacgc ggttacgcaa tcactccagc ctcttcttta
    25681 tttctcttta ttcatttttt tcaaaaaaag attgacgctg atggtatcga ttcattccta
    25741 actggatcag ttcttcaatc aaatcaccgt atttcaagcc catattttcc cataagagtg
    25801 ggtacatact gaactccgta aatcctggca tagagtttaa ttcattcagg aataattcat
    25861 ttttatttgt caaaaagaaa tcgcaccggc tcaatccgct tccacctaac atcgtgtaag
    25921 ctaacttcgc gtactcttgc gctttttgat aaacttcttc tggcacttcg gctggaatct
    25981 gcatttcgat tttattattg atatattttg cttcataatc atagaatgct acgtctttta
    26041 cgacttcacc aggcaaagtc gtccgaacgt cttcatttcc taatacagca acttcgattt
    26101 cgcgcgcttc aattccttgt tcaacgattg ctcgagaatc atactgatag gctgttgcta
    26161 aagcattttg cagctcttct cggttttctg cttttgtaat gccgacacta gaacccatat
    26221 tcgccggttt gacaaacatc ggataaagca aagaaccttc acattgatca aatacttttt
    26281 taggattttc tttccattga ttcttaagta ctggtacata aggaacttgc ggcacaccag
    26341 cagcttgtaa aatatacttg gtcatgattt tatccatggc acatgcactg gtcaatacgc
    26401 ctgcgccgac ataaggcata ttcaatgtct ctaagaagcc ttggatcgtt ccatcttccc
    26461 cgtttggccc atgtaaaact ggaaaaacga tggctccttc ttctttgatt tcgcccggat
    26521 tgatcacttt tcctgtaaag ccttcctctg tctgtccact tgggtcccat gaaagatgca
    26581 agacatcttt gctggcaggt ttttctgtta gtaatggacc tttgacccat tgtccttctt
    26641 ttgtaataaa tacgagttga acttggtaat aattataata aatggcattt aaaactgaaa
    26701 atgcggaaag aatggacact tcatgctctg cgctgcgtcc gccatatagt aaagtaatct
    26761 tcaaaagatt gtcctcctgt attctaaatc gttcagtgat ttactctcat attttagcac
    26821 aaaacaaaaa taaacgaata acttatttat aaaaaaacga gccagcttct aaaagaacga
    26881 gcgtcattta ttttttgtca gctagattat ttgtcccgtt ttttataaaa agttatgata
    26941 aagaaaagga gggatgatga tggcgcaaac aaatccacgc agccgcttgg cagaaatcat
    27001 tcgagtgttc atcaattaca atgtagtgcc aaactttgta cagcaaaaaa atcccgaaca
    27061 ggtaaaaaaa gcctttgaag aactaggccc caccttcatc aagataggac agatgctttc
    27121 tgttcgagaa gatttgcttt cttctgcctt cactcaaaca ttcaaaacat tacaagatag
    27181 tgtacctagc gacacatttt caacggtcaa aaagacgatc gaaacagaac tttctctttc
    27241 attatcagat atattcgatg atttttcaaa atcgcctttt gcctctgctt ctatggggca
    27301 ggctcatcgt gccaagctga agaacggtga cgcggtagtc gttaagatcc aacaccctaa
    27361 catcgccgaa gagatccgac ttgatcttca actatttgaa cgagcgatcc ctttgatcaa
    27421 atacattcct gaaactagcg tagttgatct aaaaggggta ctccaagaag taaaacgttc
    27481 cttgatcaat gaaatggact tcttaaaaga aagtcaaaat ggcgaacaat tctatcaaaa
    27541 gaacaatggc tggaaagaga tccgcagccc caagatttat gacgcttttt gttcgaaaaa
    27601 agtgatcgtg atggaagaaa tgtctggtaa aaacttgaat catttaatga acgccgaaaa
    27661 caaaactgaa acttttatca ctggaattca aaacaagcag ctgaaacaag aagttgcaaa
    27721 gcttttagtt gagaacttta tgaaacaggt gtttgatgat ggttttttcc atgctgatcc
    27781 tcatccaggg aatctgctat tccatgtact aacaaaagaa gaacagacac aagctagccg
    27841 aaaaactgaa acagtccatg aaaaagagtt cggttccttt gcttttcgag cctccacttc
    27901 tgcagaagac cctgttgcgc catataccat caactacatc gattttggta tgatggggca
    27961 tctttctgca ggacttaggc aaaaactcac acaagccgtt ttagcgctgt atacgaaaga
    28021 tgcttatcga atcgaaaaag cggtccttcg actttgtcag caagaaggct cgtttgatga
    28081 aagtcggttc caccaagaac tgacaagttt tcttgaacag tattatgatt ctcctattga
    28141 tgaaatcaac ttgcaagaag tattcacaca tgtcgtgacg atttgtcatc aaaataatct
    28201 gcagttcgat cgtgatatca cgcttttgct gaaagcattt gggacattag aaggggtgat
    28261 ccgcgtcctc gatcctgaag tttccctcat ggaagtcgct tcgccttttg ctcaacatta
    28321 ttttctcact catctggatg tagaagatac attgaaacaa agcggattag acttattgga
    28381 agggatgaag gctgcgccta agatccctca acagctccac catctattag aaatgtggac
    28441 ttcgggacaa ggaaaagtca atttggaatt gaaaaagcaa gacaaattat tgagcaggat
    28501 cgaaagtatg atcaatcggc ttgttttcgg tatgatttta gctgcattga tcgtagggtc
    28561 ctccctgctt gtccaagcag cacctgtaga aaacgcagag gtcgtttcct tattagggat
    28621 atttacgtat gctattgccg cattcgtcat tatttttttg gcaatcgatg cattgatcca
    28681 aatgtataag aagaggaaaa agtagaaaaa ggttgtgaca gaaagaatca gcttcaagaa
    28741 ataagaaggg gttttacata aattgtttct caaatttttg tgaaaattcg gcttattttt
    28801 cgaagaggct acttctgaaa caccgagaat aaaaaatgtg aaaaagctgt tctgcaccca
    28861 gtaataagaa caatcaaaaa gggaacatgg aacacattta tcctgtttcc ctgttccctt
    28921 tttgatgtat tcgagcataa ataaaatacc ttcgttacct actatcttct gtacttggta
    28981 aaccttagtc aattgccttt tttaacgctt gacgaaaaga catatgatct ttctccgtaa
    29041 agggttttgg accctttgtc cttatgccag cttttcttaa ctttccacta tgataacgtt
    29101 gttctagaag cgtgccgata ttcgcttcgt caaattcttt tccagagtga tggaacactc
    29161 gcacaccctt cggcaataca agtgaagcta aaccataatc ttgcgtgatc aaaaagtcat
    29221 tcttcttaac cataccgacg attttataat ccgcactatc agctccgcgg tctacgtaga
    29281 cgatttggac atgattagcg tattctttgg tcgtataatg gtccacactg gtcacgataa
    29341 ccacgtcaag accataggat tctcctaatt ttatgacatc ttcttttact ggagaaccat
    29401 ctccatcaat gaatagcctc attctttttc gccttcttcc ataaatgata aacagttact
    29461 aatccgctca gtaaaagacc aacattgatc cattttgcca agataagcag ttgtacactt
    29521 tctatcggca ctgattcatg gttcagcgtg atcaataata gatggattgg ctctcccatt
    29581 tcttttgcta aaacaaacaa aagcgtcatc aataaaaact ggtggatcac actcccttta
    29641 ttttgagtag tatacttgac aacgatccgg cgatccattg gaatagctgt atcagtttga
    29701 taatattcct tatttagcca gctgcctgtt actacgtgcc agaaactaag cagaaagaca
    29761 cctatcgcaa aaccatttgg aatcaagagg accaataaag aagaactcag tccaagcaat
    29821 tgcatagcca atggagacca tgttaaacgt ttcaaaatcc atggtccgac tatttttgct
    29881 gcaatcaagc ctgctgcata aggcaaatac ataaaaatcg aaagtttttc taatccgtac
    29941 agtatgccaa cataaaaact gctaaataaa aaaaggaaat tccctagcat tccatttaga
    30001 aaagtaaaca gatttagcca gcttggcaac ttccattcct tcttctggtg tcctaaaaca
    30061 aacacgaaca agatgaacag cagcaagaaa ccataaatag caagatcaaa aagcctttca
    30121 tcgaataaca atcgggcatt tctcaaaaga aacaataagc caaagaatat caaaaataaa
    30181 acaagctctc gcgtagctac cagatttctt ttgatgtctt tgaaatccag ttcgtaacgc
    30241 ggataatgac taaccgtatg gtaggccatg acgtaaaaaa gtgtataaat agtaaagctt
    30301 gctgcaatct gagtcggtgc aggcaactca atcgcttgat acaggagaaa tagccagata
    30361 gctgcaaata agtacttttt cccatccata ttgataaaac cttgcgtctt ttcgtgaaaa
    30421 ttgaccgtgg tattagcagg tacaagccat gctgcactca gccctagcaa aacacttgaa
    30481 aaataaaata aaggaaaata aaatacgcct aatattccaa gcaatgaacc agcaccacca
    30541 atcagcagag cgatcatcag caacgtgtaa ctatctagac caaatcgtag actgttgact
    30601 aaaaatagtc cagtcatccg aaaggcataa aacagcgtaa atggcaaaat cgtcagccaa
    30661 ttatgcgagt ctgttagtcc caaaaataaa agataaggaa taaaaataac cgtgttactt
    30721 aagataaagg gcgttgtttg tgtaaaatta cgcccaaatc gtttgagttt ttcgaacatg
    30781 acaatggctc cttaaacagt cttataacta atctgctctt cgattttagc attaaagggg
    30841 aaataaacca attttacaga atatattctt gtaaaaaaaa atacagaaag aagcataagc
    30901 ttctttctgc atttttacgg tacttgatta ctcaagcaga caagcccttc tccacaaaag
    30961 tcttgcccga cacccctgca tccggcaggt gataaagata gaaatcaatt atcctgtgcc
    31021 tccaaaatca attaggagct ctgtaaacca gagtccttct cgacccgtcg cataaattta
    31081 ctttaacaca gttcctatgt ttatgcgaga gcttttttca ttttataatc gaccatttga
    31141 aatccttgac cgatatctga aataagttct tctgtaatct caaatccttc atgcttataa
    31201 acctcgattg cttgatgatt cccctgattc actcgcaaaa agatctctct ttcctttgtt
    31261 tgcgcagcaa gttcttcata ccagcgaaaa actgaagaag tcaatccttg accacgtact
    31321 ttatttagca aatataattt acttaaataa atttttccat ttatctcttc ataagcagtg
    31381 taaccgacag tcttttcttc gctttttaat aaaaagtagg acacaccttc tgtcagttct
    31441 ttttgaatct gtttttttga ctgataagtt ttcagcatgt aatccacttg tttttgacca
    31501 atgatcggca caaaaacttc cggccatact tcttgtatga ctgccatcag ctcatccagt
    31561 tcattttctg ttacttttat tttttccata cacccacgtc cttttttaca ttataaatga
    31621 ccttgcgctt gatggaataa aaagagcatg atcatgctat acacacccac cgaccacggt
    31681 ttcaagaaaa tcacaatctg cataaattcc ctaaaagata atttcgtcaa accagctact
    31741 agacagatca agtcgtctgg cgcaaaggga agcagcaagg taacaactag tactttttta
    31801 atatctttcg tttttcttgt cagcaattca tcaaatttct gatacttttt tggcgataaa
    31861 ataagctgga caaaggctac gccatagtaa cggacaagca aaaatccgat tgcttcccca
    31921 atcactaggc caatacaact atataaaagc ccaaatccat tgccaaacat cagcatgccc
    31981 gctactgagg agatccctcc cggcaaaata ggaatgatca cttggatcac ttgcaacaga
    32041 acaaatatca tcactgcata cttgccaaat tgctggataa acgcttgcaa agacgtgctc
    32101 gatcgaaaaa tcccatgatg ataagcataa agaatcaaca caaagaaaaa aaacagacca
    32161 ataatgggca tgatctgaag acatttttgt aaaactgatc cttttttaga catctatatt
    32221 ccccacatct tttttcattt attaaaactg cctccttatg aactgaaccc caaaagttga
    32281 actatttaat ggactgtttc cgatattcta ctggagatag tccatttaac tttagtttta
    32341 tccttttgtt attataccac ctaatatact catttaattt actttgaaat atctcaattg
    32401 atcggaattt ttctcgataa aaaaactctg atttaagcac accaaaaaaa ttttctatta
    32461 cagaattatc taagcaattt ccttttctag acatgctttg aataatgtta ttctctttta
    32521 attttttttg atattgtggc atctgatact gccatccttg atctgaatgt agaatcagtg
    32581 aaccctcagt tccctttttt ttaattgctt gttgaagcat ttcttcaatc agtttatatg
    32641 ttggactagt tgatatacta taactaatta tttctccgtt aaataaatca agtataggag
    32701 atagatagat ttttcttcct tttatcttga attcagtgac gtctgtcacc cattttttat
    32761 tgggtgtatc tactgaaaaa tttcgtttta acacattctt ggcaattttt cctactgttc
    32821 ctttataaga tttatatcgc tttattcgga tttgacaggt aagtcccatt tgggacatta
    32881 attttctaac tgttttatga ttgattgtat atcctttcat ctttaacgct aaagtgactc
    32941 tacgataacc ataagagttt cttgattctt ttacaatcgc tgtaatttct tgctttatct
    33001 tgctatattt atctggctta tctaattttt ttacccagta ataataagtt gacttcgcta
    33061 attgtgcaat tgaaagtaat agattcaatt taaattcttt tttgagctga aggattgttt
    33121 taaccttgat ttcttcttgc tcaaatcttg ttcttgaatc aaggtttct
//
LOCUS       DACDRN010000024        32592 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDRN010000024 DACDRN010000000
VERSION     DACDRN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.024"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            190..564
                     /locus_tag="IWJ80_001371"
     CDS             190..564
                     /locus_tag="IWJ80_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9461112.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(678..1307)
                     /locus_tag="IWJ80_001372"
     CDS             complement(678..1307)
                     /locus_tag="IWJ80_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9461113.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1441..1513)
                     /locus_tag="IWJ80_001373"
     tRNA            complement(1441..1513)
                     /locus_tag="IWJ80_001373"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1478..1480),aa:Thr,seq:ggt)
     gene            1727..1800
                     /locus_tag="IWJ80_001374"
     tRNA            1727..1800
                     /locus_tag="IWJ80_001374"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1761..1763,aa:Asn,seq:gtt)
     gene            1755..1904
                     /locus_tag="IWJ80_001375"
     CDS             1755..1904
                     /locus_tag="IWJ80_001375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461114.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2397..3725
                     /locus_tag="IWJ80_001376"
     CDS             2397..3725
                     /locus_tag="IWJ80_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9461115.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3927..4760
                     /locus_tag="IWJ80_001377"
     CDS             3927..4760
                     /locus_tag="IWJ80_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP9461116.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4776..5513
                     /locus_tag="IWJ80_001378"
     CDS             4776..5513
                     /locus_tag="IWJ80_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP9461117.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5668..6636)
                     /locus_tag="IWJ80_001379"
     CDS             complement(5668..6636)
                     /locus_tag="IWJ80_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP9461118.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6868..7701
                     /locus_tag="IWJ80_001380"
     CDS             6868..7701
                     /locus_tag="IWJ80_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP9461119.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7735..8574
                     /locus_tag="IWJ80_001381"
     CDS             7735..8574
                     /locus_tag="IWJ80_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9461120.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8600..9160
                     /locus_tag="IWJ80_001382"
     CDS             8600..9160
                     /locus_tag="IWJ80_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9461121.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9359..11080
                     /locus_tag="IWJ80_001383"
     CDS             9359..11080
                     /locus_tag="IWJ80_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP9461122.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11245..12387
                     /locus_tag="IWJ80_001384"
     CDS             11245..12387
                     /locus_tag="IWJ80_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9461123.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12403..13614
                     /gene="thiI"
                     /locus_tag="IWJ80_001385"
     CDS             12403..13614
                     /gene="thiI"
                     /locus_tag="IWJ80_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP9461124.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14187..14834
                     /locus_tag="IWJ80_001386"
     CDS             14187..14834
                     /locus_tag="IWJ80_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9461125.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            15227..17872
                     /locus_tag="IWJ80_001387"
     CDS             15227..17872
                     /locus_tag="IWJ80_001387"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP9461126.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18182..19495
                     /locus_tag="IWJ80_001388"
     CDS             18182..19495
                     /locus_tag="IWJ80_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP9461127.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19485..20138
                     /locus_tag="IWJ80_001389"
     CDS             19485..20138
                     /locus_tag="IWJ80_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9461128.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20193..20885
                     /locus_tag="IWJ80_001390"
     CDS             20193..20885
                     /locus_tag="IWJ80_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP9461129.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            21035..21235
                     /locus_tag="IWJ80_001391"
     CDS             21035..21235
                     /locus_tag="IWJ80_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9461130.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(21489..23168)
                     /locus_tag="IWJ80_001392"
     CDS             complement(21489..23168)
                     /locus_tag="IWJ80_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9461131.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(23170..23382)
                     /locus_tag="IWJ80_001393"
     CDS             complement(23170..23382)
                     /locus_tag="IWJ80_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP9461132.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            23775..25505
                     /gene="efrA"
                     /locus_tag="IWJ80_001394"
     CDS             23775..25505
                     /gene="efrA"
                     /locus_tag="IWJ80_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP9461133.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            25502..27262
                     /gene="efrB"
                     /locus_tag="IWJ80_001395"
     CDS             25502..27262
                     /gene="efrB"
                     /locus_tag="IWJ80_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP9461134.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            27357..27554
                     /locus_tag="IWJ80_001396"
     CDS             27357..27554
                     /locus_tag="IWJ80_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461135.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            27707..28192
                     /locus_tag="IWJ80_001397"
     CDS             27707..28192
                     /locus_tag="IWJ80_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP9461136.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(28296..28889)
                     /gene="clpP"
                     /locus_tag="IWJ80_001398"
     CDS             complement(28296..28889)
                     /gene="clpP"
                     /locus_tag="IWJ80_001398"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP9461137.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            29069..29596
                     /locus_tag="IWJ80_001399"
     CDS             29069..29596
                     /locus_tag="IWJ80_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP9461138.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            29687..30424
                     /locus_tag="IWJ80_001400"
     CDS             29687..30424
                     /locus_tag="IWJ80_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9461139.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            30428..31345
                     /gene="miaA"
                     /locus_tag="IWJ80_001401"
     CDS             30428..31345
                     /gene="miaA"
                     /locus_tag="IWJ80_001401"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP9461140.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            31347..32576
                     /gene="hflX"
                     /locus_tag="IWJ80_001402"
     CDS             31347..32576
                     /gene="hflX"
                     /locus_tag="IWJ80_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP9461141.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10785 a         5535 c         6880 g         9392 t
ORIGIN      
        1 ttttcttcta gtctacacac cttaattttt ctgtctagtt aattgtagcc tatccaagtc
       61 tacactctga gatgtaagaa atatcataag atatttctta catctttttg ttttgagcag
      121 gactttagat aattttatta atatatgcta gcatgctttt gtaagaaaaa gaaagagggc
      181 gtattagtta tgtatcttaa aattacggaa caagcacaag aaagattaca aaaatatatt
      241 gatgaaggcg caacagttat attggattta gatgatggcg ttggggaata ttctaaaatg
      301 ggtgtttgct ctttagatac tagttttcgt ttacttcttc ttgataaaga acagagaaaa
      361 aatgattata aactccaatt agatagtgat attggggatg tttatataaa agattattca
      421 aaaatgtaca tggatgaaca tatgaccctt tctttagatc cacgtctagg tgtatttact
      481 cttgaaagcc caagtggttc tttagacagt catgttcaat tagtagatct acgaccagaa
      541 aaattctctg gaataaaaca ataactttct ctctattgtc cataaaacta gtctcaatca
      601 agctgtattg aaaaaacaag acaacattct taccatggta aataaaaaga cgacacattt
      661 tgtcgtcttt ttttaaatta actattttcg ataaaagatc gcatagcttg tgccattatt
      721 tctggctttt ctctattcaa ttcatgtttt ccgtttttaa taaagagtag ccggccattc
      781 gttgaaagtt tatataaacg ttttgctgct ggtttattcg catgatcttt ggtaccgcat
      841 aaaatcaatt taggaagtcg tgatgtacga agctgttttt tgcagtctaa tgtttcaagt
      901 gaacgaagta gggtaagggt ttgtttttta gatagaccta atttttcaaa tatcttagaa
      961 ggtacgcagt aaaaataaat attctggaaa gtaagcaata agcgattagg agactcaaat
     1021 tgtggagcga ctaaaacaac cttttttaaa tatggagaag gagttgcaag atagtttaaa
     1081 gcaataacag cgcctaaaga taatccacat aaaataaaag gttcctttat ctgattcaat
     1141 tgtacttcga tgtatttggt taagtcgtct aaagtagcag tgtctgtgct gtttaaatga
     1201 ttgaacaatg aaagactttt gcaagaataa ctattgggta gataagagat tacatctttc
     1261 catgattgtt cgtcttggcc tagaccatgc aaaaaaacaa ttttcatatt gctctccttt
     1321 ttatagttat catatagact ttctttgtac tattaaaata taaacattca tttcttacgt
     1381 cataaaataa aaaaacagcg cagacacatt gttaaaccaa catgttgcac tgtttaagga
     1441 agccacttgc cgggcttgaa ccggcgacct cttccttacc atggaagcgc tctaccagct
     1501 gagctaaagc ggcattatca ttaatacaaa taataggata acgaatatta atgtatttgt
     1561 caacagaatt ttttcgaaaa aatgtgtttc tctaatagga aaacagattt tagaaaaaat
     1621 aaaaataaca aaaaatgcga acggtttctt tgataatata ataaaaaaat tcaaaaaaag
     1681 tattgcgtta tttagaagaa attgatatta tattcatgta cttgattccg gcatagctca
     1741 gttggtagta gcgcatgact gttaatcatg atgtcgtagg ttcgagtcct actgccggag
     1801 taaataaagc ttatgcaaag aaaaagaaat ccaatcactt gggtttcttt ttcttttatt
     1861 attatgatga atcaagcttt ctgtttttgt cacgtatgtt gtaatcttta cgaaagagta
     1921 gagttggcta ctcataatca accatacttc ccatcatcgc taaatacgaa ataaatcagc
     1981 attcctacac caatcaaagc acaagctgca ccaagaaaag ggatgacatt cccaaagtta
     2041 tctgtattca taagtatgcc accacccaca aacaaaaagc cgccagcaat caggtttttc
     2101 attttattca tctcctttct tacgttatag tatttccttc tttcaatgag tattttgaaa
     2161 gctatcttat tgtatgatgt tcttctaggc ctcttcttta ttatacaggc cattttcgtt
     2221 atctcataga aatattcctt tttatctgaa aactattgta taccgtatga catttttctt
     2281 catgaagttg taagtatatt ctcattcaaa tgaaaatttc atcatagaag agattgtatt
     2341 tctaaattct taagagtaaa atacgaagta taacttggat ttggaaggat gattggatga
     2401 aagctattac gcctgatatg acaacaaaat ttaacaaaca gtttcaagca aatatcaaac
     2461 agcaggcatt gcagcgggca gttgtgaaga acggaattac tgcttctgct gaaaatgttt
     2521 cagcacatgt ggagaataca ccggtatttt ctatagattt ggctacgggg aaagtggcta
     2581 atcaaatgca atcggggcgt tgctggatgt ttgctgcact gaacacattc cgtcataaga
     2641 tgctgaatca attccattta aaagagtttg aattatcaca aaattacact tttttctggg
     2701 ataaatatga aaaggcaaat tatttctatg aaaacatcat tgcaacagga aaagaggcaa
     2761 tcgatagccg gaaagtagct ttcttactcg ctactcctca acaagatggc ggccaatggg
     2821 atatgatcgt ttcattattc caaaaatatg gcgttgtgcc taaatcggtg atgccggaaa
     2881 gtgcgaatag ttcaaattct cgagatttga ataactactt gaacaaatta ctgcgtaaag
     2941 atgcagtagt gttacggaaa atggttgctc aaggagaaac attagacgct attgaagaaa
     3001 agaaagaaga aatgctagaa agcatttata acttcttagc tatctcatta ggaacacctc
     3061 caaaagaatt cgattttgag taccgcgatg aagaaaagaa ctatcatctt gaccgaggac
     3121 tgacaccaca aattttttat gataaataca tcggtgtaaa attagatgat tatgttagcg
     3181 tgatcaatgc accaacaaaa gacaaaccat tcaatcgttc ttatacagta gagatgctag
     3241 gaaatgtcgt tggcgggaaa gaagtcaaat atctgaatgt tgatatggaa actttcaaaa
     3301 aattagcgat tgctcagctt gaagaaggag aatctgtttg gtttggttgt gatgtaggtc
     3361 aatcttctac tagaactagt ggcatcatgg ccttagatgc ttattcaatg gatgatctat
     3421 ttgatacgga ttttacaatg actaaagctg aacgtttaga ttttggagaa agtctgatga
     3481 cccatgcgat ggtattgacc ggtgttgact tagtagacgg gcaatcaaca aaatggaaag
     3541 tcgaaaatag ctggggcgaa aaagttggaa agaatggctt ctttgttatg agtgatgcct
     3601 ggatggacga atatacgtac caaatcgttg taagaaaaga tttattgacg aaagagcaat
     3661 taaatgcgtt caatgaagaa ccaattgttc tatctccgtg ggatccaatg ggtgctttag
     3721 catagacgag tatttcaatg aagagtatgt agtaaagata aaaagagctg gcttaagcca
     3781 gctcttttta tctttaaagt ccattccctg ttttttatgg tccctgtgct ataattctaa
     3841 ataaaggata aggattggaa ataatccttt tatcattaaa aatatgatga cgaagtagaa
     3901 taaaattggg agtagggggg aaagtcatgg atatccaaac tgttttatct gatctgaaat
     3961 taaatggtaa agtgattcca gttgttggtg gcgatgttaa tcagacctat cgtataaaaa
     4021 ccgaacatcg tgcttatttt ttaaaaatcc atccgaatgt aaaaaaagga ttttttgaag
     4081 cagaagtaga cggattaaaa gaactgtcag cattcgtacg tgtaccggat acgtatatgc
     4141 taggtgaaac aagtgaaggc gcttatttgt taatggaatg gatcgagccg ggaaaagggg
     4201 atcaacgaga tcttgctgca gctttagcta atctccatca gcaaacagcg ccacaatttg
     4261 gttttcgtaa agataactat ttgggtacac ttgttcagaa aaattctttt gaagaagatt
     4321 ggtggacttt tttctttaaa gatcggcttg aatcccagat ttctttggca gaagaaacga
     4381 atcgatggaa tgtacagcgt caagaaaagt atctgcgatt taaagaacgt gtattgaaat
     4441 cggttgaacc aaaaaagatc actccacgcc tcttgcacgg agacctgtgg agtggtaatg
     4501 tgttctttga ccagcaaggg catccagttt ttgtcgaccc agctgtttct tatgggaatc
     4561 gggaacaaga tattgcaatg agccagttat tcggtggttt ccgaccggaa tttttagatg
     4621 cttatcaaac tatatttcca ttagaaaagg gatggaaaga ccgactgccg atttaccagt
     4681 tatattatct tttggcacat ttgaatatgt ttggagaaag ttacggttcc caagtagatc
     4741 aattactaga aaatttttaa gaggagggcg tgtttttggc aaaaaaatat accaaaaagc
     4801 atgaagtttc atattatgag tgtgacatca accaaacgat gacatttcct tcgatgttag
     4861 gagtagttat caaaacgtct gaagatcaaa gcgaccagtt gggaagaggc agtgaattcg
     4921 tcaatagctt tggacttact tgggtcatca cgaattattc catggagatt aatcgtctcc
     4981 ctagagtcgg tgaagtaatc tcagtaacga ctcaagccat ggagtacaat aagtttttct
     5041 gttatcggaa cttctggatc catgatgaag atgggaaaga attagtcaaa atagaatctg
     5101 ttttcgtatt gatggatttt gtgaatcgaa aaatgagcag tgttaatgaa gagatcattg
     5161 ctccttttga gagtgagaag atcaagaaaa tcaagcgtca ggaaaaaatt gaaaagattg
     5221 attcgggctt catgttgcca tatcgtgttc gtttttatga tatcgatagt aatcagcatg
     5281 ttaataatgc gatgtatttt aattggatca ttgacgtatt aggatatgac tttttgacta
     5341 cgcatgttcc agaaaaagta attattcgat ttgataaaga agtagagtac gggaatgaga
     5401 tcgaaagcca ttttgaacaa atccctatcg aaaatggtgt caaaacaaaa catgaaatcc
     5461 gaatgcaaga acagctgtat tgtgaagcaa atattatatg gaaaaataaa taaaaaagag
     5521 gttgtgacag aagggggcaa cttcaagaaa taagaagaaa tttacgaaaa ttgtttctca
     5581 aatttttgta aattttcgac ttagttttcg aagaagctat ttttgtcaca gcctctcttt
     5641 ttaagtttag ctattagata atgatgttta gttttggata aacgaaatca attcttcacc
     5701 cgacaaacca tagtttacag caatcaatag ttttaaacgt gctttttgac tattaatact
     5761 gttacagaaa ataatcccca ttttttctag ttcttttccc ccgccttcat aatcataaac
     5821 aggctctgcg attccattga aacaacgaga aacaagtact acagggattt tttggacaag
     5881 aagcttttcc aaagcagcta aggcttgtgg agggagattc cccgcaccta aagcttcgat
     5941 taccaaaccg tctactttcg tatgggcgat ggcttccaat aagtcccctt gcatcccagc
     6001 atatgcttta acgatcggga tagtcccatc tacagcggaa atgtccagtc gctttgtttc
     6061 taaaagttcc tgcaagaata aaattcgatt cttagttact aagccgatgg gacccaatgt
     6121 aggtgttcgg aaagtagcaa catttgtggt atgggtcttt gtcacgtagc gtgcagaatg
     6181 gatctcatcg ttcatgacca ccaaaacccc cttatctagc gcttcttcac aacttgcgac
     6241 acgaatcgca ctttcaaaat tataaaggcc gtcacttcca agttcattac ttgaacgcat
     6301 tgccccagtc aaaacaattg gtaatttgtt gccaatcgtt gtatcaagaa aaaaagcagt
     6361 ttcttctaat gtatctgtac cgtgtgtgat caccactccg gatatacctg atgcataagc
     6421 ggacaagata cgttctttta ataccaacat gtgagaaggc atgatgtgtg gtgagggaag
     6481 attgaagata tcttcaacta ataattctac atgcttgggg attgtgattt ctgcatctaa
     6541 taatgggttt gccacatcag gactaacacc gcccgtgagc atgttttctt tcatggcgat
     6601 cgttccgccg gtgtgtagta caagtatttt tttcatttta cagacttcct tacgaataat
     6661 tttgcatagc cgttaaatct tattgtatac tatataaaag agttttgcta tcagtgaaga
     6721 ataacgaata gcctaactta tcataaaaaa gctgacctta ttaaaatgga aaagactcaa
     6781 ccttgacctc ctaatacaat ggagtatctg gttgagtttc ttttacaaga aagcagccaa
     6841 ggaaatcaat cacgaaggtg gccggaaatg atcagaatcg gattaacatc gtttaaagaa
     6901 catgacaaat taactggtaa aaagaataac actctttatg aatatgcagg gtttctaccc
     6961 ttagtggaat tagatactgc ctattatgga attccccgaa agtcctcagt tgagaattgg
     7021 ataagtgaag tacctgaatc atttcgcttt gtagtcaaag tctatcaagg aatcagcggt
     7081 caaggagact ggaagcctta ttatcagacc gaagaagaga tgattgcgca ttttttagat
     7141 gttatgtcgc caatggtaga tgctggaaag atgttttgct ttttagtcca attcccctca
     7201 tcattcaaat gtacgaaaga aaatgtccag tacttgcgca aacttagaaa gtggtttgag
     7261 ggttatcctg tttctataga attgcgggat tattcttggt acagcgatga atttcttgaa
     7321 cgtacaagaa actttatgtc tagagccgct ttttctttag ccatcatcga tgagccacag
     7381 ttgctgaata caacgattcc ttttgatcca tttgtcacca atccgaactt tgctcttttt
     7441 cgattccatg gtaggaatgc gcaaggatgg caagcaaatg ataaagagtg gcgtaaaaaa
     7501 agaacgttat atcgttattc agaaaaagaa ctgcaagaac tagcacatga agtcaaagta
     7561 gttgattcta agacaaaaga ggtaggtgtg atttttaata ataattctgg tggtgatgcg
     7621 gctgaaaatg cattagcact gcaaaaaata ttaaatatca agccagatgg actcaatcca
     7681 acccaaatgg atctgtttta agggcttatt ttattcaaga aaggggcgaa gtctatgatc
     7741 aaagctattt tttttgatat tgacggaaca ttggtcacca atcgttcaaa agcgttggaa
     7801 tcaacaaaac aagcaattga atatgctcgg aaaaacggca ttttatgcgg tgtagcaacg
     7861 gggcgcagcc cagtaaagat caaagaaatc attgatgaat tggaattaga tatgtatgtc
     7921 gtgtataatg gtcaactggt ttttactgca gatcgcacga ttatcgacca cccttttgaa
     7981 caaaaagtat tggaacatat tgttgaattt gcagatgaga accatcgaca aatcgttttt
     8041 ggtgcgagaa atcggttaga tggatcaacg acgatgctat taggacaatc gatttttatc
     8101 aagcgactag tcagcttttt accgcgtaaa ttcccggtac gtctgatgaa aaaaatcctc
     8161 caggttttta gccctcaccg acaaaaggat cgttatgaaa cgttagagat attaaaagaa
     8221 ccaatttatc aatgtatatt gctcagtccg gagtcagaac agaaaaagtt agaacatcgc
     8281 tttcccgagt gtacgtttca gcgttcaaat acgtatactg tagatatcat cccaaaaggt
     8341 ggttcaaaat tactaggcat ccaagctttc gcaaatgctg tcgaaattga aatggaagaa
     8401 attatggcat tcggcgatca ttacaatgat atcgagatgc tgaaaggtgt aggaattgga
     8461 gtggccatgg ggaatgctca gatcgaagta aaacatgctg cagactttgt gactcaatca
     8521 aatgaagaag atgggatata cgaaggactg aaacacttcg gtctgattta ttagtcaatg
     8581 aaagaaggaa aaaatcagta tggaagcaga gacacctttt caaaaagtag aaacattcca
     8641 caaaactttt gatccaagaa gaccagagat acccacggct tttacagaaa atgaagctca
     8701 tacacgtgct gcgttcaaaa tagaagaact tgttgaattt gttgcagcta cttcacctaa
     8761 taaagaacaa ctgcatgaag ctgttatgct gctgcacgaa gcattagata aagcagaaga
     8821 aaaaatactt ggtaaaacac agtggggaag tgatccgctg gttgaacaag tagatgcact
     8881 gacagatatc ctttacttta cttatggttc tttttcatta ctaggaatag tgcctgatcg
     8941 aatattcgaa atcgtccatc aggcaaatat ggggaaatta tttccagatg gaaaacctca
     9001 ttatgaccct gtaacaaata aagtgttgaa acctgaaaac tggcagatgg aatatgctcc
     9061 agaacctaaa atcgcggcag aaatccaacg tcagattcaa gaagcgattg ctaagaagaa
     9121 atccgctgat ataggcatgt gcacaaatga aaaagagtga tcagagtttc actatttttg
     9181 ttatctttat acgatggaaa aacaaaagat tttcgtttaa gttagtgaat gcatgaaaat
     9241 agtatgctat aatctagaat agatgtcttt tgcacgagag acgtattatt tggatttatg
     9301 ccaagcagaa cgccatctgt tgcataaaag atgcatactg aaaacggagg aaccataaat
     9361 gggaaataat gttatcatcg gaatcgtcat tgcaatcatt atagtcgcca ttgtcgtctt
     9421 tttggttagt tacttgatga agaaaaaaaa ccaagatcga ttggatgcct tagaaaaacg
     9481 caaggaagaa ttatttgact tgccagtgat tgaagaagtc gatgatgtta aaaaaatgca
     9541 tctggttggg caaagccaaa atacctttcg tgaatggagt caaaaatgga atgacatatc
     9601 tacttcctcg ttcgctgaac tagaaagtca aattttcgaa gtggaaaacc tcaatgaaag
     9661 ttttcggttc ttaaaaggaa aaaaagcagt tgttgaagca gaagctacaa tggaccaaat
     9721 ggagaaacaa gtgacggaaa tccgtgaagg tctaaaggaa ttgcgggaaa cggaagaacg
     9781 caactcacta gctgttcagc aggcacttga cgtctatgaa gaactgaaag aaaaagtcag
     9841 agaagacgag catgaatacg gaccagctct ttttgaattg caaaaacaga tcaaagtgat
     9901 cgaaaaagaa tttacgcagt ttgtgacttt aaacacttct ggagaccctg ttgaagcccg
     9961 cgaggttttg gataaagcag aaaaacatac gtatgaagtg gaagctctga tgaaaaaaat
    10021 tccaccgctg tatgaggagc tccataccac tttccctgaa caacttgaag aaatcagtga
    10081 tacttacgag aaattgatgg aagaacaata tgtattccca gaagaagatc ttgctgaaga
    10141 tatcgcaaaa gtaagtcgaa gaattgaaaa ttcattagcc aatcttgaaa aaacagaagt
    10201 tgaaacggtc gaatttgaaa accgtgaaac agctgatttg attgattcgt tatatgatat
    10261 cttagaacgg gaaatggaag ctcagagata tgtaaaaact aaccaatcaa cgattgcaga
    10321 gtatatcaaa catacaacaa agaataaccg tcaattattg attgagctgg atcacacatc
    10381 tcaaagctat acattgaacc acaatgaaat tggccgtgta cgaggtttcc aaaccgaagt
    10441 ggaagaaatg gaacgtcaaa atgaacagat gattccacag atccgtcaac acgaaattcc
    10501 ttattcagaa gtgcgcacgt tctataaaac agtcttcaaa gtcttagaag atatcgaaaa
    10561 gcaacaagta gagatcgacg actcattaca tgaattgaga aaaggcgaaa aagaagcgca
    10621 agaaaagatc gatacattcg aattcaaatt gcgcagtttg aaacgttttg tagaaaaaca
    10681 acgtttgcct ggcttgccga atgattacct agaattcttt tttgttgcta ctgaccgtat
    10741 tgaagaacta agtgttgtct tgaataaaat tcgagtaaac atggaagagg tcaatcgttt
    10801 agttgctcta tgtgaagaag atctagaatt attagataag aagacacatg atttagtaga
    10861 tgcagcagca ttaacggagc aaatgctcca atatgcaaat cgttatcgtc atacacacga
    10921 agaagtgaga gaagcaattg ataaaagcta ctatttattc aataaagaat atcactatca
    10981 agaagcatta gacgaaatcg gtacagcatt agagcgagta gaacctggtg ctttcaaacg
    11041 catcgaagat ttttatttta atcatccaga cttagtataa agcaaaaaag ttagtaaacc
    11101 agcaagagca agacggatta ttgtcttgtt cttgtctttt tttgtatatt tgaaaattga
    11161 aacaagtgaa aatttcacct gctcttgaaa gcaccttgga caaccgttat aatagaaaag
    11221 aatttcaaaa aagaggggtt tcttatgata tattttgata acagtgcaac aaccccaatc
    11281 tttccgcaaa gcttagatgc atatgtaaaa accagtcagc ggattattgg gaatccatca
    11341 agcttgcatg atttaggcaa tcaagctaac cggttgttgc agcaagcaag aaagcagatc
    11401 gctgatttga tccatgtaga accagaagaa atctatttta cttcaggcgg aacagaagga
    11461 gataactggg tattaaaagg aacgatgatc gaaaaaagag aatatgggaa tcatatgatc
    11521 atttcaggag tcgaacatcc ggccgtcagc gagacagcag aacaattaaa agaattagga
    11581 atcgaagtaa gtatcgcccc agtggataaa cgcggatttg tacgtgtcga cgaattgaaa
    11641 gaattaataa gaaaagagac agtattagtc tctgttatgg ctgtgaataa tgaagtagga
    11701 tcgattcagc ccatccagga aatcagtgat cttttggatg attttcctaa aattcatttc
    11761 catgtagatg ctgttcaggc gattggaaaa gtaccattct cgcaatggtt gacagaaaga
    11821 gttgattttg caacattttc agctcataaa ttccatggcc cacggggaac tggtttcatt
    11881 tattggaaaa agggtcgtcg tttagcacca ttactgacag gtggagggca agaaagaaat
    11941 cagcgaagtg ggacagaaaa tgttccaggt attgtggcta tggttaaagc tttacgttta
    12001 tcattagaga taaaggaaca aaaacctgag cagacacaac gcttaaaagt agctctaatc
    12061 aatgcgttga agacgtatga gaaagtgacg attttttcag aaggaactga ctatgcaccg
    12121 catattttat gttttgctct aaaaggaatt cgcggcgaag ttctcgtcca tgccttagaa
    12181 gaaaaacaaa tatttgtttc tactactagt gcctgctcct cacgtaaaca tatggcaagc
    12241 agtacattgc acgcaatgca tgtaccaaat gatctggcta catctgctgt tcgaatcagc
    12301 ttagatgaaa acaattcgct tgcagaagtc gagcaattta tgattatttt caagcagctt
    12361 tataagaaat tttctgcaat caattaaaag aggagtgttc ttgtgaaata cagtgaaatt
    12421 atggtccgtt atggggaact ttcgacaaaa ggaaagaatc gaaaaagctt tatcatgcaa
    12481 ctagcacaaa atgtcagaca agctttgaaa gactttccag aaatcaaaat ccatgctgat
    12541 cgggaccgga tgcatctttt attgaatggc gcagacgggc agctagttat tccaaaacta
    12601 gcaaaaatat ttgggatcca aaatttctct ccaagtattc gtgtagaaaa agatgtcgaa
    12661 gttttgaaac aagctgttca agacatcatg aaagaaattt atactgggaa agaaacattt
    12721 aaaattatcg ctaaaagaag cgatcaccaa tttgaattga caagtaatga attgaatcaa
    12781 acattaggta atgctgtttt tgatattttc ccgcatatcc aagttcaaat gcgtcaacca
    12841 gatattccat tacgtgtaga gattcggcgt gatggcgctt atctatcgta tgaaacgatc
    12901 aagggagctg gaggattgcc cgtaggaaca agcggaaaag gaatgcttat gctttctggg
    12961 gggattgatt ctcctgtagc agggtacttt gccatgaagc ggggagtgga aattgaagct
    13021 gttcattttg ctagtccgcc gtatacaagt gaacaagcac tgcaaaaagc aaaagatcta
    13081 acagccaaac tagcacctta tgtgggaacg atccatttta tcgaagtgcc atttaccgag
    13141 attcaagaag aaatcaaaaa gtcagtgcca caaggatatt ggatgacaat tactcgtcga
    13201 atgatgttgc gtttgaccga tgaaattcgc cgtattcgtc atggtttagt cattttgaat
    13261 ggtgaatcat taggtcaagt ggcttcacaa actttgcaaa gtatggtggc tatcaatgaa
    13321 gtaacgaata cgccaatcat tcggcctgta gcgacaatgg ataaacttga aatcattgaa
    13381 gtagcagaac agatcgatac atttaatctt tctatccagc cttttgaaga ttgctgtacg
    13441 atttttgctc ctccacagcc aaaaacacgc cctcaactgg aaaaggtcca tcaatatgaa
    13501 gagcgtttag caatagatga aatgatccaa cgagcactag ctggcttgaa gatggaagag
    13561 atacaagcag aatcgtctaa tgaaaaaact gaagaatttt cagaattgct ataagaggtt
    13621 atgaaaatag tgttttgacc agagtattag agaaacaaaa cgaaaaaata gctccatgta
    13681 ttttaccgtt tttgaactaa tgtcgaagat tagctgattg aataccattt atcaagaagt
    13741 tatggaaaag ccggttcttt tttactaact gaaaaataaa attttattta gagatcaagc
    13801 tacagttgat aaatgatata aaaaagagaa tttcccatga aatagcgagg ttgtgacaga
    13861 agtgggcagc ttcaagaaat aagaaggaat ttacgaaaat tgcttttcaa atttttgtga
    13921 aatttcggct tatttttcga agaagttact tctggaacac cgtgtatcag aggttgcgaa
    13981 aaagctgccc tgcatcaagt aataagaaca gccaaacaaa aatagttcct catattttac
    14041 gtttggttga gcttattacc gcagatgcaa gcttttgaac accgttttat ttgtctcaca
    14101 cccttttcct tcctacttgt aaccttgctc ccgtgttaga aacatgttac actaaatatg
    14161 tgaaattatt aacaaggagc gtcgttatgc gagaaaaaac agatacactg ccaaaagcaa
    14221 ctgcgaaaag attaccttta tatttacgct acttgaagat gctggatgac tcaggtataa
    14281 gtagaatcaa atcaaatgag ttcagcgaaa tcacccaaat tccttctgca acgatccgca
    14341 gagacttctc gcaattaggc gagctaggca gaagcggata tggctatgat gtcccttttc
    14401 ttatcgatgt attcaataat atccttaata ctaaagaaga aaaacgaatc gcattagtag
    14461 gatatggaaa tcttggcaaa gcattgaaac acaacaattt tagaagaaat gaaaatctaa
    14521 atatcgtgtg cgtctttgat aatgatccag cactgatcaa tcgagtaatt gatggagaaa
    14581 tgatctatcc gattgatcgt ttcgcggaga tagccaaagc aaaaaatgtg acagtagcaa
    14641 tctctacggt accaagtaaa tattcacaat ctgctattga tgaaattgtc aaaggaaatg
    14701 taaccgctat acttaatttt gcgcctgaca gagtaactgt cccagcttat gtcaatgttc
    14761 aatacattga tttgacaacc gagttgcaga cgcttattta ctttgatgag aattactcag
    14821 aagttttttc ttaggaaaat ttgagtaggc ttattgatct tgcgagaact cgtcgtaatt
    14881 ctttcaaaaa ttttttagta ctaaatgaag tggttttttc acgaccactt gttgactaaa
    14941 aaaaaaaacc attgtagaat gaagcataac gctgaacaag aggagtaact ggcacattca
    15001 ttcttagaga gagaactatc tgctgaaagg ttctcaatga caaccagtga aggtagcttg
    15061 ggagtttctg tcttgaatgg agtagagata gacgagtcgt gatcgttaac tcacttgaga
    15121 ggcaataatg attgattatt gcaatttagg tggtaccgcg taaatcatac gtcctagaat
    15181 acgaaagtat tttaggacgt tttttagttt aaaggaggaa aacagcatga cggaagaaaa
    15241 taacctatca acaaaatata atccgcaaga agttgaagct ggacgttatc agcattggtt
    15301 agaacaagat ttgtttaaac cgagcggaga taaaaaagca aaaccttatt caatcgtaat
    15361 cccgccccca aatgttactg gaaaacttca tttagggcat gcttgggata cgaccttgca
    15421 agatatgctg attcgtcaaa agagaatgca aggatatgat actttatggc tgcctggtat
    15481 ggaccatgca ggaatcgcga cacaagcaaa agtagaagcg aagcttagag agcaagggat
    15541 ttctcgttat gatctaggtc gtgaaaagtt catcgatcaa gtgtgggaat ggaaagaaga
    15601 gtatgcgagc cacatccgcg aacagtgggc taaaatgggc ttatctttag attacagtag
    15661 ggaacgtttt acattagatg atggattatc tgaagcagta cgtaaagttt ttgttacctt
    15721 atacgaaaaa ggtttgattt atcgtggtga atatatcatc aactgggatc ctcaagcacg
    15781 aacagctcta tctgatatcg aagttattca taaagatatc gaaggcgcct tttatcatat
    15841 gagctatgaa ctagcagatg gatcaggcgt agtagaaatt gcgacaaccc gtccagaaac
    15901 gatgttggga gatacggcga ttgctgttca tccagaagac gagcgttatc aagcgctcat
    15961 cggcaaaaaa gtgattctac ctttagtcaa taaacaaatc ccaatcattg cagatgaata
    16021 tgtagatatg gaattcggga caggtgtagt aaaaatcact ccagcacatg atccaaacga
    16081 ctttgaggta ggtaatcgtc atgatcttcc acgcgtaaat gttatggatg atgatggtac
    16141 gatgaatgaa ctagcaggaa aatatgctgg aatggaacgt tttgctgctc gtaaagcaat
    16201 cgtgcaagat ctgaaagaaa tcggtcgatt gattaaaatc gatacgatga tccacagtgt
    16261 gggtcattcc gagcggacag gtgtcgttgt tgaacctcgg ctgtctactc aatggtttgt
    16321 aaaaatggcg ccgttagcga aaaaagccat tgataaccaa gaaacagatc aagcagtgga
    16381 attcttccct ccaagattca atcaaacgtt tttacgttgg atggaaaatg tccatgattg
    16441 ggtaatctca cgacaattat ggtggggaca tcaaattcct gcatggtatc acaaagaaac
    16501 aggtgaaatg tatgtaggga tggaagcacc agcggatagt gaaaactggg tacaagatcc
    16561 tgatgtttta gacacttggt ttagttcagc tttgtggcct ttctcaacaa tgggctggcc
    16621 agatacggaa agcgaagatt ataagcgata cttccctact agcactttag taaccggtta
    16681 tgacatcatt ttcttctggg taagcagaat gattttccaa agcttggaat ttactggcga
    16741 acgtccattt gagaatgtct tgattcatgg attgattcgt gacgaacaag ggcggaagat
    16801 gagtaaatca ttaggtaatg gaatcgatcc gatggaagta atcgaaaagt atggagcaga
    16861 tgctctccgc tggttcttat cgaatggttc tgcaccagga caagatgtcc gcttcagcta
    16921 tgaaaaaatg gatgcttctt ggaactttat caacaaaatc tggaatgctt cacgtttcgt
    16981 tatcatgaat atcgaaggaa tgaccgtaga agagatcgat ttatctggag aaaaatcagt
    17041 ggctgaccgg tggatcctta ctcgtttgaa tgaaacgatc gaacgagtaa ctgagctatt
    17101 tgatcgtttt gaatttggtg aagcaggtcg acaactgtat aactttatct gggatgattt
    17161 ttgtgactgg tacatcgaaa tgagtaaaga agtactttat ggagatgatg ctgtttcaaa
    17221 acaaaccacc cgtagcatct tagtccatac acttgatcag atcctacgtt tattgcatcc
    17281 aatcatgcca ttcgttacag aagaaatatg ggaacatatt cctcatcagg gaaattctct
    17341 agtagtggca gaatatcctg ttgtacgtcc agagtttgat gatgaaacag cttctaaagg
    17401 gatggaagta ttgaaagaat tgattcgagc cgttcgaaat attcgttcag aagtgaatac
    17461 accattgtct aaaccaatta cgttgctaat taaaacaaac gatgctaaaa tcgatcaatt
    17521 tttagttgaa aatacaagct atatcgaacg cttctgtaat cccgaagagt tgacgatatc
    17581 aagtgagatc actgcgccag atctagcgat gtcagctgtt ctgacaggtg cagaaatctt
    17641 cttgccgtta gcaggattga tcaatattga agaagaaatc aaacgattag aaaaagaact
    17701 ggaaaagtgg acaagcgaag taaaacgtgt ccaaggaaaa ctttctaatg aacgttttgt
    17761 ttctaacgcg ccggaagaag tagtagaggc agagcgtgca aaagaaaaag attatttaga
    17821 gaagcaaacg gctgtcaaag aacgaatcga aagtttgcgt actattcaat aagaatagaa
    17881 aaagagagtg tgaaatcagc tagctattac taaaagctag ctgattgaac atcgttacag
    17941 gaaagagttt gagcctcact cttagcttcg attcataagg agatgtgaca taaattttgt
    18001 cgcatctctt ttttagtcta ctcttattat gtaggagaag gagggaggtg gatcatgtat
    18061 caagagtcaa taaagtagtt gataaaatcg ttcctctctc tgtttctttt ctctttgtca
    18121 tacttttatt tttcttatgt gttctatttg ttataataaa agagaaatgg gggaattgtt
    18181 gatgacgatt gaagaagcaa tagaatggat ccatagtcga ttaccatttg gttcacgtcc
    18241 tggccttgac cgagtcgaag ctttattaga agtggtaggg catccagaga gaaaagtaaa
    18301 aacgatccat atcgctggaa caaacggaaa aggttctact gtagcttatt tgagaagcct
    18361 attagaagag gctgggctga cagtaggtac atttacttca ccttatatcg aaacgttcaa
    18421 tgaacggatc gcaatcaatg gcaaaagcat tccagacaat gatttgatcc agcttgtaaa
    18481 aaaatatcag ccactgatca aacaacttga tcaatttgaa gcagtagctg gtattactga
    18541 attcgagaca ttaacagcaa tggcatttga ttattttttg atgaagaaag tggacgtagc
    18601 gattatcgaa gttggcttag gcggcttatt ggattctacc aatgtagctg taccaatgct
    18661 gacgggaata acaacgattg gacttgatca taccgaaata cttggagata caattgaaga
    18721 aatcgctgca caaaaagctg gaatcatcaa acaaggcgtt ccagtagtaa caggaaatat
    18781 tgccccagaa gcttttactg tcatccaaca aacagctaaa gcaaaaaatg caccgcttta
    18841 tgtatacggg gaaaattata aaataaacta tctgcatcct gatgagatgt ggggagaagt
    18901 atttgaattt tttaatgaaa taggaaagat ccctcaatta accactccgc ttttagggca
    18961 acatcaagct gaaaatgcag gagtagccat cgaattgtat tttgtttact gtcaattgga
    19021 aaagctgcct ttccgagaaa aagatgtttt gaagggattg aagaaatctc gatggccagg
    19081 aagaatggag cgtttgagta acgagccatt agttgtttta gatggtgccc ataatacgca
    19141 tgcaatgaac cggcttgttg agaatttgaa aaaagaattc aaaggatatc aaatcaacat
    19201 tttgttttcc gccttacgaa ctaaaaatgt tagagaaatg ctagaacgat tgcagcaagt
    19261 accaaatgtc catatttatt tgacgacctt tgaacatcca aaagccatcg aattaagtga
    19321 agtatcagat ttagcaagtg agagagtatc aatcgtttca ttgtggcaat tcggattagc
    19381 agatatattg gaaaagatga caaatgaaga cgtattagtt gtgaccggtt cactttattt
    19441 catttcagaa gttcgccatt tattaataca attaggagga gatcatgaag tataatgcag
    19501 tgatttttga catggatggt ttgctgttcg acacagagtt agtgtattac gaagccacac
    19561 aagtagtagc agacaagatg ggttttcctt atgataaaga cctgtattta aaattcttag
    19621 gtgtttcaga tgaagaagta tgggcgaatt atcatatgat tttctcggaa tttggcaaaa
    19681 caagtgtaca aagattcata gatgattcat accaggaaac gcttgaacgt ttttcgcaag
    19741 gagcggtaca aatgaagccc ggtgtgttgg aactcctctc ttttttagaa gaacatcaga
    19801 ttcccaaagt tgtcgcttcc agtaaccaac gagcagtcat tgaactttta ttgaagaaga
    19861 acaatctgtt tgaacgattt gagacgattg tttctgcaga aaatgttcaa cgagcaaaac
    19921 cagacccaga aattttcttg ttagctaatc agaagctagg tacagcgaag aaagaaacat
    19981 tgattttaga agattcacaa aatggtattt tagcagcttc tgcggctgag atcccggtag
    20041 tcatggtacc agatctgctt ccaccatccc aagaattgat caaaaaaaca gctggggttg
    20101 tttcttcttt acatgaaatc cctgcattgt taaaataaaa atccatctct tttcgtatct
    20161 tattttagat aagatagaaa ggagatgttt ttttgggaaa acgattgatc aaagaagtac
    20221 ctactagctc tttaccaagg gaacgtatgg aaatttatgg cgcggaagct ttatcggatc
    20281 aggagctttt ggcaatctta ctgcgtaccg gtcaacaccc ttatagtgtg atgtcgattg
    20341 ctggaaatct gttaaaaacg tttggaggac tggcctcttt acgacaggct actttgcatg
    20401 agttagaaga gattcgaggg ataggacgcg tgaaagcaat cgaaatcaaa gccttgatcg
    20461 aattaggtcg acgaattcag acggatcatg aaagagcggc tccaccggtt cgtaccagct
    20521 acgaattggc ccagcaattg attctggaaa tgaaagatca caaacaagaa catttggttt
    20581 gtatttattt ggacacgaag aatcaagtga tcttaaaaaa aacagtgttc atcggttcgc
    20641 tgaatcagtc agtcgctcat cctagagaaa ttttccatta tgctgtacgt tattgtgcag
    20701 caaggatcgt tttagcgcat aatcatcctt ctggtaatgt tattccatcg cagcaggata
    20761 tgaattttac caaaaggata caaaagtgtg gagaaatgat ggggatcact gtactggacc
    20821 acttgatcat cggacgcaaa cgatatttta gtttgagaga agaaggaatg atggaagaga
    20881 agtaagttgc tttcttgcaa aaaaaatgaa gcaagtgtac aattatattg taattttgtt
    20941 tgggaataga agttgaaaca tctgttgcac ctctatcgta cacgagcaat gttacaattt
    21001 aaattagtgc ttttggcacg aggaggatat tattatggaa aacggaacag taaaatggtt
    21061 caacgctgaa aaaggatttg gatttatctc acgtgaagat ggaagcgacg tattcgttca
    21121 cttctcagct atccaaggtg acggattcaa aacattagaa gaaggtcaag cagtgacttt
    21181 cgacgttgaa gaatcagacc gcggcccaca agctgcaaac gttgtaaaag cttaatttta
    21241 tctgtaaatc aaattgatgg tagacatcaa tttttacgag agaacaaagc gagtaacaaa
    21301 aatacctgcc acaagagaca atttctcttg tggcaggtat ttttttgtat gaaaaagtaa
    21361 ataaatttaa gaggctatga aagagccacc ttgtatcaag gaataagaac aaacaaaaat
    21421 ggcttctcat gtttttcatt tgttttctct tatattcagg acaaggtgct gatttcataa
    21481 cctcttattt attcttcatc tggtgtcatt accattggca aaatcattgg atgtctttct
    21541 gtacgttcga ataagaaagg ctgaagcgcg ccagtcatcg cttctttcaa tttatattct
    21601 gaacagtcag gtccattcaa acattctctt aaagcgtgga ataagattcg ctgagcttca
    21661 ttgatcattt ctcctgattc tcgcatgtag acaaatccac gagagagaat atctggacca
    21721 gccaagattt cttttttctt gatatccact gtcgcgacag ctaatacaag accttcttct
    21781 gaaagcaggt gacggtcacg taaaacgaca ttaccaatat caccgatccc gctaccatcg
    21841 atatacacat ctccagccac aaaatgccca gcaggacgcg cagaatcgct agttaacgct
    21901 aaaacatcgc cattttccat gatgaagcag ttttctgcag gaacacctac atcttgtgct
    21961 agagaagcat gtactttcaa catgcggaat tctccgtgga caggaataaa atattttggc
    22021 ttcatcaaac gaagcattaa tttttgttct tcttgtccac cgtgaccgga agtatggatg
    22081 ttattgattt ttccgtgaat cacttctgca ccagcttcag acagcaaatt gatcaaatga
    22141 ttgacacttg ttgtattacc aggaataggt gaacttgaaa agatcactgt atcgtctggc
    22201 tggatttgga tttgacggtg agtaccattc gcgatgcggc tcaacgcggc catcgcttct
    22261 ccttgagaac ctgtacatag gatcatcgtt tcattggcag gcaagcgatt gatttcgtgt
    22321 gcatctacaa atgtgccgtc tggaactttg atatatccta aacgttgacc gttgacgatg
    22381 gcattttcca tgcttcgtcc gaaaacagcg attttacgac cagttgccac agcggcatct
    22441 gctgcttgct gcaaacggaa gatgttagaa gcaaagctgg caaaaatgat acgtccttcg
    22501 atttttgaaa agatcttcaa aatagaatgc ccaatcgttt tttctgattt agtaaatgtt
    22561 ggaatctcag catttgtact atcagataaa aggcacaaga ctccttcttc cccgatacga
    22621 gccattttat gcaagtttgc tggttcgcca acaggagtga aatcgaattt gaagtctcca
    22681 gtcgctacga tatttccaga aggtgttttc accacgatcc ctaatgcatc tgggatacta
    22741 tgggtcgttc tgaaaaagct gacagaagtc tttctaaaac ggatgatcgt atcttcattg
    22801 atctcatgga gctctgcatc tcgaagcaag ccatgctcat ctaatttgtt tgtgatcaac
    22861 gctaatgcga gaggaccagc atagatagga atatttgctt gtttcagcaa gtagggtatt
    22921 ccgccgatgt ggtcttcgtg cccgtgagta atcaccaagg cttttacttt gtgtaagttt
    22981 tgtatgatgt agctgtagtc gggaatgaca tagtcaattc caagtaaatc gtcttccggg
    23041 aatttgatcc cggcatcaat caaaatgatt tcatcttgga attgtacccc atacgtattt
    23101 ttcccgattt cacctaatcc accaatagca aagacgcctg tctcgttatt tttgatggaa
    23161 agtttcatat taaaactcca ctaatgagaa atcagcattt tctttttcgt actctagatg
    23221 acttccttct agaagttgaa cgaattcaat attgtatggg gtattttctt ccacacgttg
    23281 gcgagcgtct acatcgcttt ttgcctcaat gtaaagagat tttgtttctt cccgtttagg
    23341 gttgcgtact tttgtttctt gataataaac tttatagatc attgttttgt ctcctttatc
    23401 tgcgtaaagc agtgatttag ctagttgatt tttttagaca acacaaaacg aggggacaag
    23461 ccacctggtt tttcatatgc tttgactaga atactggatt cgagtcaaag agacaagcct
    23521 cgtttttgct tatctgttcg gatatttttt caaccgtacc ttctgtataa aaaatataca
    23581 acaattaatt gttattctag cataaaacag caaataagta tagaaatcaa ctagattgaa
    23641 agaaaaaatc aatgtttatc atcccgctat aatcataatg acttttttat gagagaagtg
    23701 ttctattaat aggctcgttg tgctatcatt tttagtaatg aaaatgtaac aaaagattaa
    23761 aaggagatga gaaagtgaaa ctaatgtggc attataccat gcgttataag aaattcttgt
    23821 ttttcaattt catatgcgta tttggtttta ttttgattga attaggactt cctacgattt
    23881 tagcacgtat gatcgatgta ggaatccgca atgatgattt tgattatgtg aagcaacaag
    23941 ggatatttat gatcgttatt actgtaatcg gtattatttt gaatatcttg ttaggttatt
    24001 ttggttcgag gattacgact aatatcgtag cagatattcg agatgatttg ttcgaaaaga
    24061 tccaaagtta ttcgcatcag gaatacgaaa atctaggcgt atcttcattg atcactcgta
    24121 caacaaacga tgcttatcag atcatgttgt tcctgcaaaa tattttacgt atcggtttta
    24181 tggcaccaat gatgttcatt gtcagtttgt acatggtcat gcgtacaagt gtgacactca
    24241 gtttgtatgt agtaggtgca ttacctttac tattattggc agtcgtagcg attgctaagg
    24301 tgtctgaacc gttatctaaa aagcaacaaa aaaacttaga taaaatcaac agtatcttgc
    24361 gggaaaatct ttcaggattg cgtgtgattc gtgcattcgt caatgaaaag tttgaagaaa
    24421 aacgttttgc tgtagtcaat gagaattatg cttctagttc aaaaagctta ttccgtttga
    24481 tggctgttgc acaaccgggt ttttatttct tatttaatat cgtaatggta ttgatcatct
    24541 ggagcggggc agtgcaaatc gatgcaggcg agcttcgtgt aggagatttg attgcgttta
    24601 tcgagtatat attccatgcc ttgttctcat tcatgttatt tgctagtgtg ttcatgatgt
    24661 atccaagagc agctgtttca gctggacgta tccaagaagc cctagatgcc aaaccttcga
    24721 ttgtagaaga tccaaatggt atatctgata caaaaactaa agggtatatc gaatttcaaa
    24781 atgtaacatt tgcttatcca ggccattcgc aagagccagt gatacgaaat gtcagtttta
    24841 cagcctctcc aggagagacc gttgctttta ttggaagtac cggtagcgga aaatctacgt
    24901 tgatccaatt gatccctcgc ttttatgatg tgagtgaagg aaagatcttg atcgatggag
    24961 tagacgtgag agattatcgg ctaagcaagt tgcgagataa gattgggttt attccgcaaa
    25021 aagcgctgct atttactgga acgattgcag ataatttgcg atacgggaaa gaagatgcta
    25081 cgcaagaaga aatggaacgt gcagctgaga ttgctcaagc ttcagaattc atttctcgaa
    25141 aaccagatgg atttaatgaa catttatcag aaggtggttc caatttttca ggtggacaaa
    25201 aacaacgatt ggcaatagcc agagccatca tacgccgtcc ggaaatctat atttttgatg
    25261 acagcttttc tgcattagat tatcaaacgg atgcgaagtt acgtgctcgt ctgaaaaaag
    25321 aaacgacaga atctactgta ttgatcgtag cacaacgtgt gggaacgatc atgcatgcag
    25381 ataagatcat cgtattaaat gaaggagaag tcgtcggtat ggggacgcat cgggagttgt
    25441 tggaaaaatg cccgatttat tatgatattg ccgcttctca attatcaaag gaggaattag
    25501 catgaaaaaa accttttctt ccttaaaacg tttagctcga tatatcagac cgtatcgtga
    25561 gacttttctt ttagtgatcc tatttacagt tctgacagtg gctttcaacg tggctttgcc
    25621 atatgttaca ggacttccta ctacagaaat cagccgtaat attgccaatg gcgaatcaat
    25681 caatttttct tatatattca aatgtctcat ttggatcaca gttgttggaa tagggtattg
    25741 tatttcgcaa ttactttctg gtgttttgat gacaaatgtc gttcaaagtg ccatgcatga
    25801 tttgagaaga gacatagaag aaaaaatcaa tcgtcttccg gtttcatatt tcgataaaaa
    25861 ccagcaagga aatattttgt ctcgtgtgac gaatgatgta gatgcagtca gcggtgcttt
    25921 acaacaagct tttattggta tcgtaaatgc gatattggga attacgttgg ctgcagcaat
    25981 gatgttttat atccagccga tgatggcgct gatctctatg atcatgattc caacatcgat
    26041 ctggatctca aaacgagtga tcaatgcttc gcagaaatat tttcaaagca tgcagaattc
    26101 tcttggtgag ctaaacggct acgtccaaga aaatatgaca ggttttagtg tattgaaagt
    26161 atatggacgc gaaaaagaaa cattggaagg attcaaaaaa gttaatcaca gcttgaaata
    26221 ctttgggttt cgtgcagcat tcatttctgg cttgatgatg cctctagttc aattaactgc
    26281 ctatgctact tatattggta tggctgttct aggaagcttt tatgttatta ctggggcgat
    26341 cgttgtcggt caactacaag cattcatcca atatatttgg caaatcagtc agccaatggg
    26401 gaacgtgaca caattgtctt cagttttgca aagtgcatca gcggcaacga aacgtgtatt
    26461 cgagatttta gatgagccag aagaaaaaga aaacaaagta gatgcttcac tgccagaaac
    26521 gattttagga gatgtgacat tcgatcatgt cgattttgca tatgatccga agaaaccatt
    26581 aatccaagat ttgaattttg aagtgaaagc tggtcaaacc gtagctgtgg tcggtccgac
    26641 cggtgccggc aagacgacat tgatcaatct attgatgcgt ttttatgacg tagacaaagg
    26701 agcaataagg attgatggaa tcgacaccaa atcaatgagt cgcagtgatg tgcgttcgct
    26761 atttggtatg gtcttgcaag atgcatggct ttatcaagga acgattgcag ataatatccg
    26821 cttcggaaag cttgatgcga cagattatga agtagttgat gctgcgaaaa cagcaaacgt
    26881 agatcatttt atccgaacga tgccagatgg atatgatatg gccatcaatt ctgagggaga
    26941 caatgtgtct ttaggacaaa aacagttgct gacgattgcc cgagcagtgg tctccgatcc
    27001 taaaatcttg attttagatg aagcaacaag ttcagtagac acccgtttag aagcgttgat
    27061 tcaaaaagca atggatcgcg tgatggaggg acggacaagt ttcgtcatcg ctcaccgttt
    27121 atcgacaatc cgtgatgcag atctgattct agtgatggat caaggtcaga tcattgaaaa
    27181 aggaacgcat gagagtttat tggcacaagg tggattttat gaaaaactgt ataacagcca
    27241 atttgctgag gaagcagaat aagtcatata agaataaaac agtttaaaac ttattttttg
    27301 ttctctattc ttgataatgg taaagtaaag ttatcaagaa aagtgaggac acggagatgg
    27361 aacagttttt aaatgaaaaa attactgaag ccaaaagaga agttgatcgt ttaacgcacg
    27421 tctgtcagac gaagcctttt gattcagctg atttttttga aaatgagttt caaatcgaac
    27481 gcttggcagc acaaatagaa gtgtacgaat taattttaaa aaaagtaata gaagaaaata
    27541 tagatgtaca ataaaaacag gtaaaacgtc agttaccaga tactgtaaat agacatctgg
    27601 cctgagcagg ttttacctgt tttttttctt atgaaataaa tatatgctat gataccttta
    27661 ttcaatagga ttagtttcta caaaaaatga aaggagaaaa ataaatatgg aacggtttat
    27721 agcatcaaat gccacagtga ttggtgatgt gacattgtca gaagatgtga cgatctggta
    27781 tcaagctgtt ttacgcgggg acagtaattg gataaaaata ggacaaagaa cgaatatcca
    27841 agatggtaca atcatccatg tcgatcatga tgcacctgta gatattgcgg agaatgtgac
    27901 ggtaggacat cagtgcatgc tacatggctg tacaatcgaa aaaggggcgc tgatcgggat
    27961 ggggaccacg atattgaatc atgcggttat tggtgagaat agtttgattg gtgctggatc
    28021 attagtaaca gaaggaaaag ttattcctcc taatgtcttg gcttttggtc gcccagctaa
    28081 agtgatccgt cctttgacaa aagaagaaat acaaaaaaac aaagaaaaca tccagcatta
    28141 tgtcgagata ggtcggatgc atgcgcaagg aaaattcaag gagcaaagat aggcagtagc
    28201 atccataaaa taaaaaagga tggtcagttt gagttgacca tcctttttta tttggtttag
    28261 ataagagagc ttaaaaaagc ttcctttgaa acagattaat ttaatgaaga actgttttcc
    28321 atgacttcat cgatcaatcc atatgctttt gcttgttcag ctgtcatata gttgtcacga
    28381 tctgtatctt tttcaatcac ttcaagcggt tgtccagtgc gttcagctaa aattttgttt
    28441 aagcgttcgc gtgtttgcaa aatatggcgt gccgcaattt cgatttcagt tgcttgccct
    28501 tgagcgccgc caagaggttg atggatcatg atctcagcat ttggtaaagc aaatcgttta
    28561 ccttttgtac cagctgtcag caagaagctt cccattgaag cagccatacc cataacaatt
    28621 gtttggacat ctgctttgac aaagttcatt gtgtcgtaaa tagccatacc tgctgtaact
    28681 gaaccaccag gagaattgat ataaagatag atatcttttt cagaatcttg agcgtctaag
    28741 aaaagcaatt gggcgatgat cgaattagca agatcatctg ttacttggcc gcttaacatg
    28801 atgatacggt ctttcaataa acgtgagtag atatcgtaag ctctttcacc tcgtgaagat
    28861 tgttcaatga ctgtaggaat taaattcata atagagttcc tccttgattt cgtttagaaa
    28921 tagtgtacca taatttagtt tctaatacct aaaatgtagt atacattttt ggtcaaaaaa
    28981 ggtcaaataa aaaacttagg caaattagtc gcataaaccc gctttatggt atgataagca
    29041 gagaaacaaa acaaggagga acaacagatt gatagttggt attactggcc ttatcggagt
    29101 gctgacaggt gctgtagttg tctatatcgg catgatactg caaatgcgtc acttggaaaa
    29161 taaagaacag cgtagacgtg aattggaatt gaaagtcaaa gaaatcgaga cgctgaatca
    29221 attaaataaa aagatcaacg agatcttgca aaaacggaca acgctgatgg aagaatatgt
    29281 cagctttgat gcatttgatg attgttatat cacgattgac gattttgcgt atctccaatc
    29341 attttcagcg caaaatagtt tttatctgcc aaattatatt ttagacgagc tatttaagaa
    29401 tatctcccat cgaaaagtga ttttatctcc agatgaaaca atgaagattg gtggatatgc
    29461 ttataaaggc ggacgcgtga ttttagagaa ttttacggat agtctgactg aaatgatcaa
    29521 tgaaaaaaaa gctcagttga agactatgac gcgccagccg attggtttct tctcgaaaga
    29581 tgatgtttgg agctgatatt ttgaagcatt gccgttgcac atgagagcgt ggtaatgctt
    29641 tctttttttc ttaatgatac aatagaactg aacggagtga agaaagatgt tacaaatcta
    29701 tgcccatcgc ggaagcagcg gcacccaccc agaaaatacg cttcctgcat ttgcagaagc
    29761 tgtacgagta ggtgcagatg gtatcgaact agatgtccat ctaagcaaag atggttattt
    29821 gatcgttatg cacgacgaag aagtggaccg gacgactaac ggtaaagggc tgattcgaga
    29881 aaaaacattg gaagaactca aaaaattgaa tgcagggggc aaattcagca aagaatttcc
    29941 agcagctaaa gttcctgaat tgagagaagt gttaggactg ctcctccaaa aaaattaccg
    30001 agggatgcta atgattgaaa ttaaaactga tcagtatgaa tatcccggta tcgaagaaaa
    30061 attagccgaa acattaaaaa gtagagattg gccttttcaa catgcatatt gcagctttca
    30121 tttggattct ttggaaaaaa tcgcattact ggaaccggag atacagctgg acttattgat
    30181 gagtacttct gataaaaaga tccaactagc agcagaaacc ccttatatag aaggagtaca
    30241 tccaaaaaat acttggttgt tcgaacatcc agtccaggca gctgattttc caaaagcaat
    30301 tcgtgcatgg acgattaacg atgaagaaga aatggccaaa tgtcaagaac tcaaaataga
    30361 tggaataata actgattttc cggagaaagc tcttcggtgg aaacaatcaa cgaaaagtag
    30421 gtaaaatatg gaaaaagtat tagtgatcgt cggtccgaca gcagtcggta aaacagcgct
    30481 aagtgtggaa ctcgcacaac tatttggagg agagatcatc agtggtgatt cattgcaaat
    30541 ctataaaaat ctggatatcg gtacagcaaa aatcaaagaa gaagagatga ataatgttcc
    30601 gcatcatttg attgatgtga tcgaacctac tcaacgttac tcagccgctg attttcagca
    30661 ttcaggtcga caattgatta ccgagattgc gaaccgaggg cacctaccta tcatagctgg
    30721 tggtacgggt ctctatatcc aatcccttct atatgattat cagttaggag cgacagaaga
    30781 agcgaatcct gagattagaa aaaagtatga aacgttagct gaaacaattg gttcccaagc
    30841 attatggaat cagttgaaag aaaaagaccc ggcagctgct gagaaaatcc actggaacaa
    30901 tcaacgcaaa gtgataagag ctctggaagt tttagagacg acaggacata gtatcacggc
    30961 acctaaagaa gagccaaagc aattgtatga ttacctcatg attggattag atacgaagcg
    31021 tagtattctt tatgaacgaa tcaatcaaag agtcgatttg atgctagcag aaggtttagt
    31081 agcagaagcg cgtaaggtgt atgagcttgg agaagtccag gccagtcaag gtattggata
    31141 taaagaattt ttcccttatt ttgaagggaa agaaacattg gaagaagcaa cagaacaagt
    31201 caaactacat tcgagaagat acgcgaaacg tcagttgacg tggtttcgta accgtttgga
    31261 tgcacagtgg tttgatctta taagtgagcc aaatcaaaaa agtcagattg agcaaacaat
    31321 caaaaaatgg ctggaggcgg aataaaatgg aagaaaaagt gatcatcgta ggggtagaaa
    31381 ccgaagcaaa ccagcgttac ttttcagagt caatggaaga gctagttcag ctgacgaata
    31441 ccgcatcagg agaagtcgta ttcacaatta cacaaaagag accacaagtg gatcggcaaa
    31501 cgattatcgg taaagggaaa ttacaagaat tagttcaaca agcggatgcg catgaagcgg
    31561 atttgatcat ctttaatcat gagctaactc cgagacagag ccaactgatt accgatgctg
    31621 ttggtcttcc ggtaattgat cgcgtgcagc ttatcttaga tatttttgcg atgcgtgctc
    31681 gttcgaaaga agggaagctg caggttgaac ttgctcagtt agaatacctg ctgcctcgtt
    31741 tagtagggca agggaaaaca ttatctcgtc ttggcggtgg gatcggaaca agagggccag
    31801 gtgaaacaaa gttagaaaca gaccgccgac atatacggaa taaaattttt gctgttaaga
    31861 aagaattgaa agaagtagaa gctcatcgag agcgcaatcg ccaaaaaaga aaaaatagcg
    31921 agatttttca gattggactt attggctaca cgaatgccgg gaagtcaact atcttgaatc
    31981 tattgacaca agcggatact tattcgaaag atcaattgtt tgcaacgtta gatccattga
    32041 ctaagagatg gcgttttgct gaaggatttg agattactgt aacggatacc gtcgggttca
    32101 tccaagacct gcctacgcaa ctgatcgatg ctttccattc tacattagaa gaaagtcaaa
    32161 atatggatct tttgctacat gtagtcgatg ctagttctcc agatcgtatt ttacaggagc
    32221 agactgtctt aaaattgatg gatgaactga atatgaaaga aatgccgata ctgactgttt
    32281 acaataaggc agaccagatc gacccagcga tgttcacgcc tacgctattc ccaaatgtat
    32341 tgatttctac tcagacgaaa gaaggtaaga tggcgttgat cgaagcaatc aaacgtcagc
    32401 tgatggaact aatgacccct tatacacttt ctgtgccaag caacgaagga cagaaattaa
    32461 gcgagttgag aagacaaaca atggttttgg acgaagcgta catggaagaa acaaacgaat
    32521 atgaagttaa agggtttgca aaaaccaatt caaaatggtt aagaaaaaac caataaaatt
    32581 ttattttttt aa
//
LOCUS       DACDRN010000025        31663 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDRN010000025 DACDRN010000000
VERSION     DACDRN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.025"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            100..963
                     /locus_tag="IWJ80_001403"
     CDS             100..963
                     /locus_tag="IWJ80_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9461142.1"
                     /translation="MLMSIRFVDFGYKISHSIIGLAIVMFSLLVAPYVQLIKWSVMGM
                     LIHFILLSSILMATASDPKMGNASLYGFSYLFIVYSLPKDSLNKNFFTQTSSLLFLFF
                     CWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQEKIWMLIYAFGISLLIVAGEYVPFQR
                     LMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGSLIGCVLFIGISQFIPFNWVGILGGL
                     ALGVCSTYRYKTVFNSFGALAITASLFGVPGAVTIRIFENILGVCLGIMYIGVTEILI
                     RKIREKHGLNH"
     gene            complement(1041..1334)
                     /locus_tag="IWJ80_001404"
     CDS             complement(1041..1334)
                     /locus_tag="IWJ80_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP9461143.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            1502..1816
                     /locus_tag="IWJ80_001405"
     CDS             1502..1816
                     /locus_tag="IWJ80_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP9461144.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            1837..3816
                     /locus_tag="IWJ80_001406"
     CDS             1837..3816
                     /locus_tag="IWJ80_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9461145.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            3829..4155
                     /locus_tag="IWJ80_001407"
     CDS             3829..4155
                     /locus_tag="IWJ80_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP9461146.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            4171..4689
                     /locus_tag="IWJ80_001408"
     CDS             4171..4689
                     /locus_tag="IWJ80_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461147.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            4689..6041
                     /gene="celB"
                     /locus_tag="IWJ80_001409"
     CDS             4689..6041
                     /gene="celB"
                     /locus_tag="IWJ80_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9461148.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6125..7630)
                     /locus_tag="IWJ80_001410"
     CDS             complement(6125..7630)
                     /locus_tag="IWJ80_001410"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP9461149.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(7722..8312)
                     /locus_tag="IWJ80_001411"
     CDS             complement(7722..8312)
                     /locus_tag="IWJ80_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9461150.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            8491..10139
                     /locus_tag="IWJ80_001412"
                     /pseudo
     CDS             8491..10139
                     /locus_tag="IWJ80_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
     gene            10188..10907
                     /locus_tag="IWJ80_001413"
     CDS             10188..10907
                     /locus_tag="IWJ80_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9461151.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            11506..12453
                     /locus_tag="IWJ80_001414"
     CDS             11506..12453
                     /locus_tag="IWJ80_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9461152.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            12465..13385
                     /locus_tag="IWJ80_001415"
     CDS             12465..13385
                     /locus_tag="IWJ80_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9461153.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            13426..14883
                     /locus_tag="IWJ80_001416"
     CDS             13426..14883
                     /locus_tag="IWJ80_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9461154.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            15228..15842
                     /locus_tag="IWJ80_001417"
     CDS             15228..15842
                     /locus_tag="IWJ80_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9461155.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            15835..17559
                     /locus_tag="IWJ80_001418"
     CDS             15835..17559
                     /locus_tag="IWJ80_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9461156.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            17556..19007
                     /locus_tag="IWJ80_001419"
     CDS             17556..19007
                     /locus_tag="IWJ80_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9461157.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(19480..21633)
                     /locus_tag="IWJ80_001420"
     CDS             complement(19480..21633)
                     /locus_tag="IWJ80_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9461158.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(21635..22681)
                     /locus_tag="IWJ80_001421"
     CDS             complement(21635..22681)
                     /locus_tag="IWJ80_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9461159.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            22831..24117
                     /locus_tag="IWJ80_001422"
     CDS             22831..24117
                     /locus_tag="IWJ80_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP9461160.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVE"
     gene            24133..26835
                     /locus_tag="IWJ80_001423"
     CDS             24133..26835
                     /locus_tag="IWJ80_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9461161.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            26820..27677
                     /locus_tag="IWJ80_001424"
     CDS             26820..27677
                     /locus_tag="IWJ80_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9461162.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(27748..28605)
                     /locus_tag="IWJ80_001425"
     CDS             complement(27748..28605)
                     /locus_tag="IWJ80_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9461163.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            28816..30987
                     /locus_tag="IWJ80_001426"
     CDS             28816..30987
                     /locus_tag="IWJ80_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9461164.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            31055..31396
                     /locus_tag="IWJ80_001427"
     CDS             31055..31396
                     /locus_tag="IWJ80_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9461165.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
BASE COUNT        10263 a         5552 c         6491 g         9357 t
ORIGIN      
        1 cgatcattat tcatcgtatt gtttgccatt tgttttatca ttataattac aacattattt
       61 gaatcgaagc ataaaccata cgctgtggtt ttattctgta tgttgatgag tattcgtttt
      121 gttgactttg gttataagat ttcccattcg atcataggtt tagcaatcgt catgttctca
      181 ctattagtag caccgtatgt ccagctgatc aaatggtcag ttatgggcat gttgattcat
      241 tttattttac taagcagcat attaatggca accgctagtg atcccaaaat ggggaatgct
      301 tctttgtacg gcttttcata tctctttatt gtttattcgt tacctaaaga ttcgttgaac
      361 aagaatttct ttacacaaac aagtagctta ttatttttgt ttttttgttg gttttcagtt
      421 ctcctttaca gaaaacatcg agaaaagaac aaagacaaaa gcttgtttaa agagattttt
      481 ttgaaaggca tgtattcgca ggaaaaaatt tggatgctca tctatgcatt tggcattagt
      541 cttttgatcg ttgcaggaga gtatgtccct tttcagcgat tgatgtgggc agggtttgca
      601 ttttcttcta ttgtatctag ctatggattg atgtcgattg gtttcaaaga acgagcagta
      661 gaccgaatca ttggctcatt gattggatgt gttttattta ttggtatcag ccaatttatt
      721 ccttttaact gggtcggtat attaggtggt ctagctttag gtgtttgttc tacctataga
      781 tataaaacag tttttaattc ttttggtgct ttggcaatca ctgccagttt attcggggtt
      841 ccaggagcag taacgatccg catttttgag aatatccttg gggtatgttt aggaatcatg
      901 tatattgggg tgactgaaat acttatccga aaaataagag aaaaacacgg tttgaatcat
      961 tagatgagat cgaataaaaa ataaggaaaa agtgaagata cacatggatg ataaattgct
     1021 atcattccat gtgtatcttt ttacggtaaa tcagtgaaac gttcgacttt catttttaat
     1081 ttatactttt tacggagttc gtttatttga gccatcattt cggatttgtg atgcttttcg
     1141 attgcttctt catctttcca acggtcgatc agtaaaagag attctggatc ttctagtgga
     1201 aagtaatagt catatttctc gtttccttct tctgcacgaa tcgctttgac aatgtttctt
     1261 tcgaccattt cttctgcaaa ttttctggct tgtccatttg tacttgtata caaaatattc
     1321 actgtgatac tcactaaatc ccctcctgaa aaaattctat cactagtata tcacggtgcc
     1381 agaaattatt attcaagata tgatacacat tttttccgca atgctacgga aatatatatt
     1441 ttgaatagaa aacgcttaca agatatgctt ggtttataaa aataaaagga gtgattgatt
     1501 tatgaaaaaa gcattgatta tttgtgcggc aggaatgtca tcctcgatga tggcctcaaa
     1561 gacaacggat ttctttaagc aaaaaagaga agaggtactc gtagatgcag tttcagctac
     1621 agaaggcgat aatatgatta agaatagtga ttttgattta tttctgatca gcccgcaaac
     1681 gacgatgttt ttggataaat tcaaaaaatt aggagaatct gttggaaagc ctgttgttag
     1741 tatccctttc caagcgtatg tcccaatccc tacaggcatc cagaagttag cagaggtgat
     1801 cgaagagaac atatgatgga ataggagtgg atgataatga aacagaagga aaaattgctg
     1861 ttgagatatt tgattgatca caagaaggag tatgtcacca gtcaacgttt agcttctgaa
     1921 ttgttattat ctgatcggac aatccgtaat tacttgcagc ggatcaaaga gttagtagaa
     1981 aaaaacggag gtaagatcat tgctaaacca ggttatggct accaactgca tattttacaa
     2041 agactgacat ttgacttgtt tttgagtcgg caagaaatag tacctggata tgctagagaa
     2101 ccgcaaggat tttatgagtc cgaagatcga caaaagtata tattgaacca attgcttttg
     2161 gaagatgcca ttctttttat ggatgatttg gcagaagagc tttatatcag tcgttccagt
     2221 ttgacaaagg acatgcaaga aatcaaggag cgattgattc catattcact aaaaatcgtt
     2281 tctaagcatg gtcaagggac atggatcgaa ggggaagaaa gaaatcgacg gcattttatc
     2341 atggatactt tttttggaaa ccattatgga aattcattga aagaatattt agggcaaagc
     2401 cgttttttcc cagacatcag ttttgaagag ctagtcatta tcattctcga tgaaacaaga
     2461 gaagctaagt taaaagtttc ggattttatc atacagaatc tgactttaca cttggctttg
     2521 gggatcaaac ggctccgaga agggtttgaa atcaaggaat taggcatcga acaagaagtt
     2581 tctaagcgag tagagtatca agtggcgcag cggatcgttc aacgtattga ggtaatcgca
     2641 ggtgtatcgt ttcccgaaga agaaattgct tatttgacat tacatctcat ggctaagtcg
     2701 aatcacaaag aaaatcaaaa tgatcaggaa ctatcaaagg aattagcgaa agttctgaaa
     2761 agattgtctc aggaatttca tttttcatta gtagaggatc tccagctttt caatggacta
     2821 ctcgatcatt tgtcaccgat gatgattcga ttgagccgag gtattcgcat tgagaaccca
     2881 ttgactgagg agataaagaa agaaaatccc aaagtctttg acgcagtaaa aaggcatttc
     2941 agcaacatgc cggctttgaa aaactatacc atcaatgaag atgaatgggc ctatttggct
     3001 ttgcatctca tggcggcatt ggaaaaagaa agagctgcgc ataaactaca tgcattgatt
     3061 atctgtgcaa cgggttacgg cagtgcccaa ctattaaaga atcgcgtagt cagtgaattc
     3121 ggtaaaaata ttacagtggt aagtgtaaaa ggctattatg agatcaatga aacaacttta
     3181 aaaggaatcg acctgattat ttcttctatt gatctttcga caatgttttt caaaattcca
     3241 gttttacatg tcagtatttt tttgaatgaa gatgatgttc gtaaaatacg aaaggtagtt
     3301 ggagaatcta taccctatca ttcctcagat agaccgatac cagcattttc tctacagcaa
     3361 aaaaagcaga tttacaccga acagttgtct gaaaaatttt tcaagactta tacgtacgaa
     3421 ccgaaaaagg aagaaattct tcatgatttg ctgcaaaagc tgagcataaa tgaagaagaa
     3481 ggatatataa gagaaatgcg aaagcagatc cagcaacgtg aaaatatggg acaaatcata
     3541 ttcagtgata cagtggcagt cccacatccc gctgttccag ttggaatcga aacaaaggta
     3601 gccgttgcat tgattccttc tgggatgcag tgggatcaat atcaagaaat tcattttgtt
     3661 tttttagttt cgccttctta tgtcgaaaat gaaggaatta cggtcgtgac taaagcaatc
     3721 gttaggttag ttgaccagct aaaagtacag caggaaatct tagctgaacc tatttttgag
     3781 aatttcagca gtaaatttat caaactgatc gaataaggag gaaaacagat ggctgaaaaa
     3841 ataaacagtg aaacactaca agtgacttct tttgatatca ttttccatag cggaaatgca
     3901 cggacactga tacatgaagc atttcgctcg atgcgtaaag agatgtttga agaagtagaa
     3961 gagaagttgc gacaagcaaa tgaagagatt cttcatgcgc atcagtcaca aactcaactt
     4021 ctgaaagaat atgccagcgg gacaaaaata gaaatggaaa ttatcatggt ccatgcacaa
     4081 gatcatctga tgacgacgat gacattattg gaagttgcac aggaaatgaa ttatttgtat
     4141 caaaaaaata aatagaaagg aaggatcatt gtgcaggata cagtaaaaag gaaaatcgaa
     4201 ttagaaaaaa agcaacttag tttgaaaaac atgtatttca atcgttatct aatcgtacgt
     4261 tacctgactg cctttttctt tttcatgaac atgcaatgga tgattctgct tctatcagct
     4321 aaaagtttag gaagtagttt accgatgatt ctgattttag ctattttgcc tgcagtagga
     4381 gaacaagtaa agctttatcg aaaacaccaa acgaacgttc catggacaaa gtggtatttt
     4441 ttgttccaga gtgtctttaa tttattagtg attccagtgt tgtttacctc aggatttaca
     4501 ttcttgtatc cttttatggc aaataacaat ggaggacagt tatttgtttt cattcttgtt
     4561 gtaagcggta tttttgccag tgcatttata cagtatcgtt taaagaaaat ttcactcaac
     4621 caagaccaac agtatatccg tattaaacaa tatgaaaaag cgttatattt aggaaaggag
     4681 aataactaat gtttgatttt ttgcagaaat acctgatggg acctatgggg aaaattgcgc
     4741 aacttaaaat cgttcgggct gtcatggcag cggggatggc ttctattcct ttcacgattg
     4801 taggctcgat gtttttggtt ttaaatattt tgccattacc gttcccattt ttagaaggat
     4861 tttttaatgc aacttttttc aaagttagtg acttgtacat gatcgtcaat acaatgacga
     4921 tggggatttt atctatttac tttgccattg tgtttgctta tgaattaacg tcaattgaac
     4981 gagatgaaca aaaattaaat gttaatccgc tcacgggggc attactatct gtttttgcat
     5041 tctttatgtg tatccctgaa ttgatcatga ctgatggtaa aatcagcttg attgcttcta
     5101 tcaatgagac agaaactgta gtaaatggta ttaggatggg ctcttttgtc gaacgtttag
     5161 gaacttcagg aatctttaca gctattatta tgtcgattat tgcggtcgaa ttgtattgta
     5221 tgtgtgtcaa gagaaattgg gtcataaaaa tgccagatgt tgttccgcca ggagtttctc
     5281 gttctttcac tgctctgatc ccaacatttg tcattgcgtt tgtggtgatg atcatcaatg
     5341 gattattagt tgcactagga acggatatct tcaaaatgat tgctatccca tttgggtttg
     5401 ttacgcagtt gacgaataca tggcttggtg tcatggtgat ttatttcttg atccatgcat
     5461 tgtggatcgt agggatccat ggagcgaata tcatcacttc tttcttgacc ccgatcgtat
     5521 tagctaatat ggcagctaac gcacaaggcg caaattatcc gttggcagga gaattcaaca
     5581 attcttatgt aaccgttggt ggatcaggtg cgactttagg attgatcatc tttattgcat
     5641 tcctcgcgaa atctgatcag ctgaaagtgt taggaaaagc ctcacttgta ccaggattat
     5701 tcaatatcaa tgaaccaatc attttcggga tgccaatcgt ctacaatccc tatttagcgg
     5761 ttccgttttt cttagctccg atggcttctg ctacactcgc ttattttgcc attcggctgg
     5821 aaatcgtcaa accgatgctt gcacagatgc cgtggccttc accagtcggg atcggagcat
     5881 ttgttggtag tggtggtgat tggaaagctg ctgtgttagc agtcatttgt gcaatcatcg
     5941 cattcattat ctggttccca tttatcaaat tctatgataa taaattatta aatgaagaaa
     6001 gagaaaaggc agcagaatta gctagtgaaa agttaaaatg atttctaaga aaaaaggaac
     6061 tgtgacaaaa aatcacagtc cctttttttc ttagaaatgg gaaaccacta gtatataaat
     6121 accattattc ttcaatcaac cgcttcacga ttgcataatc cccttcataa ggttcatcta
     6181 caccattgat tttttgatag ggatccacgc cttttcccgc aataacgact gaatcttgcg
     6241 gagcgctttc tgctaaagcg cgtcgaatcg cttcaggacg atccatttca taatgaacga
     6301 tcaaatctgg attagtaatt gcttcattga tttcttccgc gatttttttc ggatcttcaa
     6361 atgctgggtc atctgccgtc aaaaaggcga tgtcagctgt ttctgatagc acttttccaa
     6421 aatcatgtcg acgggagatt gctttgtttc ctgggctgcc taatacaaca attacacggc
     6481 catctggatg ttcatttttg gcaaatttca gcaatgtttt caagcttaga taattatgtg
     6541 catagtccac atagacatgc gctccattgg attggatcaa ttgatccatt cggcctggta
     6601 cacgagcttc ttttaatccg tcttgtgcat cttgactgga ggcacctgtt agtgctgcgg
     6661 caatgatcgc actggctgca ttttcttggt tgaatcctcc agccagcaaa atatcataag
     6721 cagtatcgca gatagctaag cgatcttcat gagaagataa agtaaaaccg tgagtacctt
     6781 tatctgaacg gatcacttga taattgcttt cttccgaacg accataagtg atcgttggaa
     6841 tctggaatag atcggctgtt tccctaagta aatcaaaata gtcactttca tgatttaaaa
     6901 caactgtttt tgaattcaag atcaattgtc gcttgcaata gaaatagtca tcaaatgtcg
     6961 gatgttcgat cggactgata tggtcaggcg agatattcaa gaagattccg acatctaaag
     7021 tcaagccgta tacgcgttgt gttttgtaag cttgtgaaga aacttccatc actagatgag
     7081 tcataccatt ttcaactgct tctgacatca tacgatataa atctaatgac tcaggcgttg
     7141 tcaaatggga tttgaaatat gttttgccat ctaacgttgt attcatagta gaaagcaatg
     7201 ctgttttttt atttgttgtg tgatccaaga ttgcttttgt aaagtacgca gcagttgttt
     7261 taccttttgt tccagtgaat ccgatcactt tcaatttatt ttgcggatag tcgtaaaaag
     7321 ccatagcaag aagcgccatt gctttacgga tatccgttac gatgattcct actgctgaat
     7381 tttcgtatgg ttgttctgat acataatatt gaataccctg ttcgattgct gaatccaaat
     7441 attcttcttt gaaattcatt cctttacaga aaaacaaagt agaagcatca gcttttcttg
     7501 aatcataact taactgaaga aattcagctt tttctggtac atcaagatgc cagcctcttg
     7561 ttgaaacaaa ttcttttaat agattttctt ttaatagtaa atgacggatg tcttctaaag
     7621 agaagctcat tgattgatcc ctcgctttat tcaatttctt ggtttattat agcacactct
     7681 ttttaatata tcgtttgttt ccgctatgac aaaatccagc tttattcaaa ttttcttgac
     7741 acaggggaca agcggatgag tattttcata aacaatgaac tgactgtaat agataggaga
     7801 acgaatagtg tctccaacaa tccaaaaaca taataattca aaaagacgat aagactattg
     7861 gtaagtaaaa gtccttgcgg cattggaagc cgaaaaaatt tgtggaaaat aatcggcggt
     7921 atcgttatac cgccatttga ggccccgact gtgtacaatg tgtaaacccc aagactgaac
     7981 aaaagacttc ctgaaatcag tgaaaagaaa tgtgaggaga tcatttccac ttttggaaca
     8041 atctctaaaa caacaggaaa aagcagactt cccgctacag tcttaaaaaa tactgatttt
     8101 cctaatagga cagcggctag aagcagcatc ataagattga tgctccaaac agtaactgag
     8161 cgttggatag aaaaggccgt ttcaactaaa tgtccgatgg cgcccactcc ggcggaagct
     8221 atatcgtgag ggagcaagaa ccaattcaat ccaactgtga taaagaaaag acctaaaaat
     8281 ataacgggta gtttttttat ttcttgtgtc attgatatct cctataaatg ttagccataa
     8341 aaggctgtat atttctttac tttaccaaaa aaacgaaacc gaattcaaaa aaacaattgt
     8401 tcttataaaa gttatataaa atcgattgcc agtttctgtt ttaccaatta aaatgatagt
     8461 aatgttttgt agaaagttgg aatctgaccg atggataatc aaagaaaacc agcattagat
     8521 caattaacac cgcaagaaaa aatggcacga ggctctgctt ggatgaccgc cagtaatatc
     8581 atttcaagat tgctaggcgc tgtctatatc attccctggt atgcttggat gggcgaaaat
     8641 gctaaggctg ccaatggatt attcaatatg ggatacaata tatatgcatt gtttctactg
     8701 atctctacag caggaatacc tgctgcgatt gcaaaacaaa ctgcacgcta taattcattg
     8761 aatgaatatg gcacttcacg acgattattt atccgtgcat tacagatgat gggcgggtta
     8821 ggcctgttgt ttgctttgtt catgtacatc gcctctcctt ggctagctca tgcttctggt
     8881 ggcggagaag aactgattcc aacgatgcgt tctttgagta tcgctgtatt ggtttttcct
     8941 tgtatgagtg tgatacgagg gtattttcaa ggcaatcaag aaatgatgcc ttatgcgctg
     9001 tcgcagattg tagaacaggt ggcacgtatt ttttatatgc tattggctac attcatcatc
     9061 atgaaagtca tggatggtga ctatgtgacg gcggtcacgc aatcaacttt cgcggcattt
     9121 atcggtatgc tagcaagtat tcttgtcctt ttgtattttt tgaagaaaca tcaagcgtat
     9181 acatcggcgt ttattcacta cagcgaaaac aaaataaata ttgcaacgaa ggaattgttg
     9241 ttagataccg ttaaagaagc gattccgttt attattgtgg gttcaggggt aacgatcttc
     9301 aaattagtcg atcagttcac ttttatgaag atcatgtctg catccaccga gtattccaat
     9361 gcacagttac tggatctttt ttcgattttt agtgccaatc cagataagtt gacgatggta
     9421 gttattgcat tggcaacatc gatcgcgtct actgggttac cgttgattac agaagctgtt
     9481 acgttgaaag atcgagtagg tctggcaaaa ttgacaagcg gaaatttaca gttgttttca
     9541 ttttttatgt ttccggcgac atttggtgtt atgttacttg cctatccgct aaatacatta
     9601 ttttatacac cggattctct aggaagtaat gtactgatcc aagcaagttt tgtaggactc
     9661 ttcctaggac tatatatgct agtttccaac atgctgcaag gaatgtttga aaacaaggcg
     9721 gcaattaatt atttggttat tgggtttcta gtgaaactcc ttcttcaata cccagccatt
     9781 cgtttatttg aagtatacgg ccctttattg gcgacaatga tcggatttgc tgtgtcttgt
     9841 accttgatct taaagagaat ccatgaagta gcccgcttta aaccaagttt tgtctggcgc
     9901 aggacacatt gctgatcttt attcttacct tgattatgct tttggcttcg tggatcacaa
     9961 agatgatttt tggtgctttt ttaaacgaag atagcaaatt ccagtctcta ttgttgattc
    10021 ttgtagtagc aggtataggg gctttagtgt atggttattt agctttgaaa atccgcttgg
    10081 cagaaagact actaggacca ggaatagcgc gtttacgaca taaattaaaa ataaaataag
    10141 tgaatcagaa gagctagatt gatgctcttt tgaaatgggg gaaacgcatg cgtttagata
    10201 agtttttagc agatatggga cttggcagcc gcaaagaagt caaaggatta atcaaaaaag
    10261 gaacgatcga agtcaatgga caagtggtga aaagcgataa agcgcaagta aatgaattcg
    10321 aagatgcggt tacttattta ggagaaatca ttacgtatca aaaagatttt tactacattt
    10381 tgcataaacc tgcaggagtc atttctgcta ctcaggataa tcatgatcaa accgtaatcg
    10441 atttattgga agaccaggat tatcgggaag atctttttcc agtcggccga ttagataaag
    10501 acacagaggg cctcttgatt ttaacgaatg acggggtgtt tgcccatcag ttactatcac
    10561 ctaaaaaaca tgtggagaaa gaatatctag ctgaggttag cggtataatg acagaagaag
    10621 atatccgttt atttgcagaa ggactgatta ttgatggcga tgagcagaca ttgccggcaa
    10681 aactccaaat tgatcaagta gatcaagaga cagaaacttc aattatccgt ctgattttgc
    10741 atgaaggaaa gtttcaccaa gttaagcgaa tggtcaaagc agtaggaaaa gaagtcttat
    10801 atttaaaaag aatccgtatg ggaaaatttt ggctgcctaa agatttgaat aaagggcaat
    10861 atcgacccat gaccaaagaa gagctagaac aagtgagaga aaaataattg aatggatagc
    10921 taaaagaata agcagagtag ttgtagcgcc atctgaaaag cagacttgtc cgtctgactt
    10981 tcggatggct tttttgtgaa atttcggctt atttccgtag aagctacttc tggaacaccg
    11041 tttgttcgga ataagggact gtgacaagac ttttgttaca gtccccattg ttccttgtga
    11101 ctcagagtta gatagtaagg acacgatctc cctaatactc aggccaaaac gctgaattca
    11161 caacctctga taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa
    11221 aatatgagga actattttcc cgtttttttc tctaatactc aggccaaaac gctgatttca
    11281 caacctctga taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa
    11341 aatatgagga actattttcc cgtttttttc tctaatactc aggccaaaac gctgatttca
    11401 caacctctcc tatactttgt ttcattttcc ctatactttg tttatttatt ctcattattg
    11461 aaagcgctat aattacgttg cgaatgaatc gaaaggggtt tttagatgaa aaaacgaaag
    11521 gggttcaatc atttttttat gaatctatgg cgttataaag cacttgtcct gatggctgta
    11581 ccgggtatgc tttggatgat ttttttcttt tacatacctg taatggcgaa tgtcgtagca
    11641 ttcaaaaatt ttcatatttc agcagatggg ttcttggcta gcttgaggga aagtccttgg
    11701 gtgggttggg aaaatttcaa atttttattt tcttcggatc aagcatttct catcactaaa
    11761 aatacaattt tatataatgt agcatttatc cttttgaatc tgctgatctc agtcgtattt
    11821 gcaatcatta tgagcgagtt gagaaataaa cgactcgtta aagtttatca aacgatgtcc
    11881 ttactacctt attttctatc ttgggtcatt atcagttact ttgtttacgc ttttttaagt
    11941 cctgacaaag gaatattcaa tcagtggatc actggacatg gaggagaagc tgtcaattgg
    12001 tataatgaac cgaaatattg gccgtttatc ttggtgttca ttggtacatg gaaagggatt
    12061 ggttataaca gcattattta ctttgcatct gttatgggaa tcgatccgac ttattatgaa
    12121 gctgctatgg ttgatggagc tagcaaatgg cagcagatca aacatgtgac gataccacaa
    12181 cttattccac taatgacgat tttaacgatt ttagccgtag gaaacatttt ccgagctgat
    12241 tttggcctgt tttataacat tcctaggaat tccggtgcat tatatgatgt gactcaagtt
    12301 ttggatacgt atatctacaa tggcctgaca tccacaggag attttggtat gacggctgct
    12361 gcaggacttt accaatcagt tgtcggcttt gtcttgttga tgatcacaaa tacgatcgcc
    12421 cgacgttttg atagcgattc tgcattattc taaggaggaa aaaaatgaaa aaaaagaaag
    12481 tggagagagt ccaagtccgc tctttcaata agacggctaa ttttttctct aatataatga
    12541 ttgcactctt tgctatttcc tgtattttgc catttatatt tgttatcgcc atctctttta
    12601 ccgatgagtc catattgtcg actcacgggt acagtttttg gccagaaaag ttcagtatat
    12661 ttggttatac gtacttgttc gaccagatgc aggataaaat tttccaagcg cttttcgtca
    12721 ctgtacttgt tactgtttta ggtacactga tcaatagcac ggctacttct ttatatgctt
    12781 atgcgatttc acggtcaaac tttccattcc gtcgtttttt taccgttttt tgtttgatta
    12841 cgatgttgtt ctcaccaggg atggtggcca attatcttgt tatgacaaat cttcttcagt
    12901 taaaagatac gatttgggca ttgatcttgc caatggctgt cagtcccttc aatattattg
    12961 tgatgcgcac attctttaaa cgttcggtct ctgattcgat tattgaatcg gcacggatcg
    13021 atggagctag cgaactgcga atatttgttc agatcgtttt accgttagct gttccaggta
    13081 ttgcgacaat cagtttattt gccgcattgg gttactggaa tgactggttc aatgctttac
    13141 tatacatcca agatgataag ctagttccac tacagtatct tttgatgaaa atccaaagta
    13201 atattcaata tctgacacaa aattcaggag caggaagcca attgacaggc ggattagcct
    13261 cagttccagg agaatctgcc agaatggcga tcgtagtcat ctcgacgttg cctattgcga
    13321 tcagctatcc atttttccag aagcattttg tcaaaggtct aacaatcggc ggagtaaaag
    13381 aataaagaaa caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa gttatggaag
    13441 aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc atgcggaggt
    13501 tctggttcta aagaccaggc agaagctagc aattcggaca ctttgcagat gtatcaaatc
    13561 ggagataaac cagataattt cgatcaattg atggaagtag caaacaaacg aatcgaaaaa
    13621 gagatcggtg taaaagtcaa tatcaattac attggctggg gcgattacga aaagaaaatg
    13681 aacgtcatca tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa ctatgtttcc
    13741 aatgcccaaa aaggagcctt tgctgatttg actgaattag cgcctaagta cgctgaaaaa
    13801 gcttataatg atttagatga agcatatatt aaaggaaatc tggtcaatgg aaaattatat
    13861 gctttcccag tcaatggaaa tgtctttgct caacaagtac ttacttttaa caaagcttta
    13921 ctagataaat acgatctttc aattgatgga atccaatctt acgctgatgc agaaaaagtc
    13981 ttgaaggaat tccatgaaaa agaaccaaac acggcagctt ttgccattgg acaaggcttc
    14041 aaagtccaag ggaactttga ttatccttta gggaatacgt taccatttgc tattgatctg
    14101 gatggagatg attcgaaaat catcaaccaa tacgataatg aaacgtatat cggactgtta
    14161 cgtacaatgc atagctggta caaacaagga ctcattccaa gtgacgctgc aacgagcaat
    14221 aaggattatc cactagaagg aaatacttgg ctaatgcgtg gagaaacaca aggaccgtat
    14281 gattatggcg atacaatttt gacaaatgca gcaaaacaag aacttgtctc aaaagcaatt
    14341 actgttccac taaaatctac agctcaagca caaatggcaa attttgtcgt atcaaatact
    14401 tcaaaaaata aagaaaaatc agtagaactt ctagggctgc ttaacagtga tccagaatta
    14461 ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga tggttcagat
    14521 gataaaatca aattgctaga tggttatcag ccaaatacac atatgtctgc ttggaataca
    14581 ggaaacaaca agattctata tacacaagag tctattacgg atgacatgat tgccaaacgt
    14641 gatcaatcga ttgctgatgc agaaacttca ccgattttag gattcagctt caacactgac
    14701 agtgtcaaaa cagaattaag taatatttct aatgtcatga atcaatattt agatggattg
    14761 aatacaggga ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc tttggacaag
    14821 gctggctatg ataaggtatt gaaagaaatg caaaaacaat atgatgagtt ccgacaagaa
    14881 taataatgaa taaagacaaa ggggttgtga caaaagacgt gtcacaacct cttgttccga
    14941 ataaacggtg ttcaaaagcc agtttctcgg tattaagcta aatatatgta aaaatgtgga
    15001 aagccatatt cacaaaattt ttcttactac tcgaaactaa gcggctattt cacaaactct
    15061 tggtaaacgg tgttcaatca gctaaccctc ggtattcgct cacaaaacga caaaatatga
    15121 ggaactattt ttccgtttgt ttctctaata atcaggtcaa ggtgctgatt gcccaacctt
    15181 tttttgtata atgagaaagt ataaacttaa tgtttggagg agaagatatg ttagggaagg
    15241 ttttggaaga gttatttatc cggatctggg tcattataaa actaaccttg tatttctgga
    15301 tatatacatt tgccggcggg atcatctttg gattaggtgc ggcatggaaa acagtcaatg
    15361 aactatttta tttgtacgga ttcgagtata aagaaatcac aataaaaaga ggctggaata
    15421 tatataaaag aaatttttta agaggcaatc tgctatttag tttattttta tctggtactg
    15481 ctttattgag ttacaattta tatttatctg ttcaaatcaa agggctcatt tttttagcta
    15541 ttgattttat tttacttttt gccttgctct gcctatttgc tacttatcaa tacagtatga
    15601 ttttagatag tgaatatacg atctctgttc ctaatctgct aaagctaagt tttatttcgg
    15661 ttttttccag cttttcgtct tttctaaaaa taattattgg tagcggtata attttaggcg
    15721 tgacttggca attcaaagga ttgatcctgt ttggtgtcat tggtcttttg acggtttgga
    15781 atggtacgat gacaacacat tggcgtgaag aattagataa acagttggaa agttatgaat
    15841 aagtttcgga ttctattttc aaaaggaacc ttactaaatc gtctgttgcg gaaatacgcg
    15901 gttatcatga tcggtgtaac gatgactgct accgttattt ttagtatcca tacttgggaa
    15961 caaaatcaaa aacaggcaga aaacatgaca agtgatgccg ttcaaagcac aagcagaatg
    16021 ctgaatgata aaacaacctt gagtaggatc atcaaaaacc agcttgtagg gaattccgaa
    16081 aagatcgaaa atgtgacgac ctacttaaca aaacccatag atcagtatct gatgtatgta
    16141 tacgaacagc aaaacagtac cgatgaatta gtttctttcc caaatcaaat caaagatctc
    16201 tacgcaaatt atgaagaact aagtgcgata tatatcgtgt tgaatcaact accggagtat
    16261 tatgaatctt ctagagaaaa caaaggtggg gaaataaaaa aggggacacc aaaattacta
    16321 gacgcctttt atatcaagtt gcctatcaca caaggaggag ctgagtcagg aagtattttt
    16381 attggttttc aaaagaaaga actagatatg attttagaaa atctaacgtc attcaatgga
    16441 ctctctttat acatgatttc tggaacgaca aatcgactat acacattcca tgataataaa
    16501 atagctaaag aaacgtttga aagacaagaa atgatcatca cagagagtct gaagaaaaat
    16561 tcacaattgc cgattgacga tctggaaaaa aacaattttg ttcaacacca agaactgtct
    16621 gatgattatc gtatattagc tgtactagat aaaaaaagtg taaaaatcga aacgggaaaa
    16681 aatcttgccc cgctgatcat tggtatcatt attttagaca atatactgct tatctttctt
    16741 tataaaacat tcaaacgata ttcacagcaa gtatctatcg tcatgcaggc catggatcaa
    16801 acagcagcag ggaatttaga gacgcggatc gacataacaa acacggaata tgaactgaag
    16861 gaactttcaa tcggtatcaa cgaaatgttg gatagcatca atcagtttgt agaagacatc
    16921 tacaaattag aaatcaaaca gcaagacgca catatgcggg cgcttcaagc acagatcaat
    16981 ccacactttt tatataatac attagaatat atccgaatgt atgcaatcag cgaaggaagt
    17041 gaagaattag cagatgttgt gtatgcgttt tccgctttgc ttcgaaacaa tacgaatcaa
    17101 gagaaaacga tcactttaaa agaagaactg gacttttgcg aaaaatatgt ctatttgtat
    17161 caaatgcgtt atcctaatcg agtagcctat cattttatga tcgatcctga tttggaaaag
    17221 atcgaagtac ctaaatttgt gatccagcca ttagttgaaa attattttaa acacggtatt
    17281 gattttactc gattcgataa tgcgctaagt gtcaaagtct tgcaggaagg caaacgggta
    17341 aggatcatca tcaaagataa tggaaaaggg atgactgaaa aacggctaaa acagattgaa
    17401 gaaaaactgt ctcatcctaa agtggaactt cacggttcta tcggtctgca aaacgtgaat
    17461 gaacgtttac gtgccagctt cggcagcagt tattatatgt cgcttgagaa taatgagaca
    17521 ggtggactga ccgtttctat tacattcaag gagggatgag cgtgtatcaa gtgttattgg
    17581 tagatgatga atacatgatt ttgaatgggt taaaaaagat aattgattgg caaagtttag
    17641 gctttcaaat agtagcaaca gcagagaatg ccaaagaagg actagctgta cttgaacagc
    17701 gacagattga tttagtagta acggatgtga caatgccaga aataaatgga ctggaattta
    17761 ttgaagcggc tcaaaaagaa cgtcataatt ttgaatttat gattttatca ggataccaag
    17821 aattcgatta tttgaaagga ggtatgcaat taggcgccgt gaattatttg atgaaacccg
    17881 tgaataagtt cgaattggtt gaaagcctca agaaaatcaa aacacggtta gatcagcaaa
    17941 atgaacaaaa aaatcaacaa gaaatctatc aagaactgtt attcagtcaa tggttgaatg
    18001 aagaattgga tgaaataagt gaagaagaaa taataggaca aataagtgag aagcagcata
    18061 ggatagtatt gattcaactg gcaagaactc atgatactct tatcaataga tggttgcaag
    18121 aacggcaaca aatcttttac tatcaacgaa attacggaga ccgtgtattg atcacgttgt
    18181 tgttggaggc agatgaagtt gatggcttct gttgttttgt tcaagaaaac tttcctgatc
    18241 aggaatggct gatcagtatc ggggaagaga cagattcttt ggacaaaatc cctgaaacat
    18301 tccaacaagc gaaagacaat ctacagttgc atcaatttta tggagataaa gacgagcatg
    18361 ttttttacgc agtacaagca gaaacaaaag atcaaaccat cgatttttca agtttcagac
    18421 gcgtgctgca aaataaacaa ttggaagtcg ctcaaaaaat gatcactgat ttttttgaac
    18481 agtttcagtt agcagccatg atgccggaag atatacgata ttccagtttt ttactgttta
    18541 tggagattca acgggaattg attgatctgg aagatgagga atatttacaa ggaatcgaaa
    18601 aaatccatca ggcaaaaacg gtgcaagaac ttcagcagct acttctgtca ttcatcaaaa
    18661 agcatcagag acagaaaaag tacagtgaca atgtggaaaa agtaatcgta atcctgcacc
    18721 agcattatca agaaccactt acactcaaag aagtgtcaga aaacctgcat ttaaatgtca
    18781 tgtatctagg acagctgttc aaaaaagaaa cgaaaaaaag tttttcagct tatttgaacc
    18841 atttacgtat ggaaaaagcg aaacaactgc ttttgcacag taatcaaaac atcaatgaga
    18901 tagcaagtga gattggctac aacaacacca cgtatttctc taaattattc aagaaaatcg
    18961 tcggcagatc ccctaaagag taccgagaaa acagtggaga atcataaaag aaaaagccaa
    19021 tgtaatgaaa taacttataa aaaattgtct agaaatctaa ttttgggctg ttcttattac
    19081 ttgaggcaag gcagcttttt catgacctct cgataaacgg agttcaaaag ccagtttctc
    19141 ggtattaagc caaatttatg caaaaatgtg gaaagccatt ttcacaaaat ttttcttaat
    19201 actcgaaact aaacagcttt ttcacgacct ctccactaaa cggtgttcaa tcagctgacc
    19261 ctcggtatta gctcaaaaaa tggcaaaata tgaggaacta ttttgccatt tgtctctcta
    19321 atactcaggt cagaacgctg atttcacaac ctctcaataa acggtgttca aaagcttgca
    19381 tctgcggtaa taagaccaac caaacgaaaa atatgaggat ctatttttgc ttggctgttc
    19441 ttattacttg atgcaggaca gctttttcac aacctcttgt taattatagt ttgataagga
    19501 aaaaggcaaa tcttttgctt gatatttctt agggtgaggg atgatcccta attggtaaga
    19561 gatcgtgccg cctttcagca aatcttgatg ggtaaaatat aatttcgtat attctttgtc
    19621 catccgtttg attttatgga taaattgttg ctgcggccga ttaggcgttg cttcaatcgt
    19681 caattccttt ccatttgaca aaagcaaaac agccttgtca attaatggca taccgataac
    19741 atattcaccg gttccaggcg tgacaggata aaatcccaga ctattgaaga tataccaagc
    19801 ggccatactt ccattgtcct catctccagg gtaacccatt ggagaatcgt cgaaggtctg
    19861 agtcatcaac tgtttaagca gtggctgtgc catttcaggt tgtcctatat aactgaacaa
    19921 gtaaggatag tgaaaacttg gctggtttga aatagctaat tgtccaaatt ctatcgcggc
    19981 catctcactc atttcgtgga tctcaaaacc atatcctcct gggtcaaacg ttgggggctg
    20041 attgcacagc tcaatgagtt tttctttata tctctcactg ccaccatatt ttccgatcag
    20101 tcctgaaaaa tcgtgataaa cagcaaagct gctttgccaa gcacttcctt ctgcataatc
    20161 ttgtccccaa cgattagctg aaaatggttc tctgaacttg ccatctttat cttttgaacg
    20221 cataaatcct gactcatagt caaagatatt ttgatagttc aatgattgct gctgatattt
    20281 cttcgtcatt tcctcttctt ctagtacctt ggcaacttgg ctaatacaaa aatcgctata
    20341 agcatagtct aatgtgtgat tgactgattc gtggtagtga ttaggaacat agccatatgt
    20401 caagtaatct tctgttcctt ggcgtccata atttggatgt ttgctttgaa ccgtagcacc
    20461 tttttccatt gccgttagaa attcaggcat caagtctcgt cggatatctt ttacagcagc
    20521 atctgcaatg accgcatcga tcaatgtccc aggcataagt ccacgttcat caggagacag
    20581 ccattttggc aaatagccac tttctctata gctgttcaaa aaaccttcca gcatttcttc
    20641 gtatttttct acagcaatca aagaataaag aggataaacc gttttatacg tatcccaaaa
    20701 cccattattt gtatagagaa caccaggttt tatagatcga ctagtcgtat catagtggat
    20761 tttttgccca ctaggatcaa tttcataaaa tgtttgtgga aataaaaaca cccgatataa
    20821 attatgataa aacgtccgca attgttgttg atcatggtgt gtgagctgta tacgatcaaa
    20881 ataggactgc cattgttctt gactgtgagc aaggtaagtc tctttgcttt ggttttgttc
    20941 tagttccagg ttataagctg cttgttccga gctgataaaa gatgtcccaa atcggatagt
    21001 ttgttttttt gtctgttcaa aatacaacgg gatcgagtcg ctatttttgt tggaaacagc
    21061 taactcatca attgttaaag gatattcaaa tctcaatata aaataaaaag aaaaatcagg
    21121 atcttctgaa ccagcaaaat gtgtgatttt tccttccact gtgtaaggat ctataacaaa
    21181 gagctgatgg cgtcccggaa aggataaaag taaaccgctg tcatttttgc tgtaatcaat
    21241 agataagatt ccgccataca tgctaggtat caatgtagaa cagatgccat aacgtaattg
    21301 acagatcgac agatgagtcg ggcaaaatat actttcttct gggcggtaag aactttgagc
    21361 atggaaaagg gtgttctcct gtagttttcc attgatcggc gtaaacagca tatgactgaa
    21421 atcacccatc caaggacttg gttgatgggt aagacggtag ccttgaaaaa cgcggtcatc
    21481 aggatgaaac caccagctgc cgttttgatc cgtagtttgc ggtgcaaaat aattcatccc
    21541 aaaaggaacg ccagtataag gctggcagtt cccattagag aaatttggct gattcgctgt
    21601 tgcatgtcta gtatctattt gaccgatatt cattttattt ctcctttgtt gattgtcttt
    21661 tgatgaatga aactggaaat ttactatcgg atggtcgatc ttttgtctct aaccaagtga
    21721 ccaaagcatc tcccgcaagc ttacctaact gttcaaaatc ttgtgcgatg gttgtcagtg
    21781 gcgggtcaac aagagctgct gcttggatat catcaaatcc gataattgaa aaatcttcag
    21841 ggatatgata accatgctgt ctcaagagat tcatggtctg gatagcgaca acatcgtttt
    21901 cacaaacaaa agcagttact tcatacttag tgatataaga aagcaattca tctaatgtcg
    21961 cgcttttgtc atccagtgaa gtgtggaaag tcaaaccagc tttgtccaat gcttcaaggt
    22021 aaccaaggta acgctgtcga acggattgtg gatgagtctg cgtaccaaac aaataagcga
    22081 tttttgaatg tccctgttcg atcaaataag aggcagcaag cgcaccgcct tggaaattat
    22141 cggaaagaac ggtaggaaaa ggtaactcat agattttttt atctaatatg acgacaggga
    22201 attctttcaa tgaaagctca aaaagtaagt caagatgttg ttctgtatca agagcataat
    22261 aaatcaatcc gtcaaattca ttgattagct cttttgctgt tttattttgt agaaagtcaa
    22321 gcgtcgtcat catgacatca atctgtttat cttgcatcac agggttcaat cccttgctaa
    22381 aatcaccaac agacaaatca gcgacatatg gaagaagaaa cagaatccgt cgtgtttttg
    22441 taggagaagc aagaatctgt tccggagaag cggatttaac gaaactgcct ttcccgcgaa
    22501 cgcgataaat aagtcctaat tgctctaatt cagtcaatgc acgtttggaa gtgattctgc
    22561 ttactttata ggtacgtgaa agttcttttt cagttggtag ttggctgtct acaggtaatt
    22621 caccagttaa aatagccgtt tgcaaatcat taaatattgt ctggtacaat ggttgattca
    22681 tataaacacc tcttaatgat atatcatatt caaataatac cataatggat gaaaaagtaa
    22741 ataaaaagat attttttgaa agcgtagaca taattggctg tatgaagtat aatctttaaa
    22801 gatatatcaa atagaaagaa ggttttatta gtggcttata aagatatacc aaaatcggtg
    22861 cagaaattta tggaagatat cacacaaaaa tgtggggata tccatgcgga ctgggcaaag
    22921 aattttacag cagcatttgc caatactctg ttgacaacag tcaaaaagca aaatgacgga
    22981 accacctttt tattgacggg agacattcca gcgatgtggt taagagactc tactgcacaa
    23041 gttcgtcctt atttagtgat tgcaaaagaa gatgaggaat tagctgacat gatcagcggg
    23101 ttagtcaaaa agcaattttt ttatatcaat atcgatccat atgcgaatgc cttcaacgaa
    23161 gaagctaacg gtgctggcca ccaaacagat catacaaaaa tgaacgattg gatctgggaa
    23221 agaaaatatg aaattgattc tttatgctac ccaatacagt tggcctatct tctttacaaa
    23281 aatacaggaa aaactgatca attcaatcaa tcttttattg aaggtatcaa aaaaatcttg
    23341 gacgtgttca aaacagagca agatcatgaa cactctcctt atacatttgt acgagacacg
    23401 acaagaaaag aagatacgct gatcaatgac gggcgaggtg caaaaacagc ttttacagga
    23461 atgacatggt ctggtttccg tcctagcgat gatacttgcc gttatggata tctggtacca
    23521 tccaatatgt ttgcggtggt cgtgttaggt tatgtggaag aaatttttca aacgatactc
    23581 gatgataaag aaattgtaca gcaaacacag aaattgaaga aagagatcca agaggggatt
    23641 cagaatcacg gacgaacggt taatcaaaaa gggcaaacga tttatgcata tgaagtagat
    23701 ggaatgggaa acgccacagt catggatgac agtaatgtgc ccaatctgat cgccgcacct
    23761 tacttgggat attgttctga acaagaggaa gattatctgc aaacaagaag aacattgttg
    23821 agtaaagaaa atccatattt ctatgaagga aagtacgcaa aaggaattgg atcctcccat
    23881 acgccagaaa aatacgtttg gccgattgca ttagctatgg aaggaatgac aacgtcagac
    23941 aaatctgaaa aagagcggat attgaattta cttgtagcaa ctgacgcagg aacacacctt
    24001 atgcacgaag gattcgatgt gaacaatcct gaaaattata cgagagaatg gttttcttgg
    24061 gcaaatatga tgttttgcga attagtcatg gattactttg atattagagt agaatagtag
    24121 gaggtatcgt aaatgaaaaa gaaagtatat atcatttctc atagccactg ggatcgtgaa
    24181 tggtacatgg cttacgaaca gcatcacatg cgactgattg aattgataga cgatctacta
    24241 gagctatttg aagttgatcc gtccttcaac agttttcatt tggatgggca aacgattatt
    24301 ttagatgact atcttcaagt aaggccagaa aaaagacagg caatccaaca agcaatcaat
    24361 gaaggaaaat tgagaattgg accattttat atcttgcaag atgattttct tatcagctcg
    24421 gaatcgaacg tgagaaatat gctgattggc atggaagaaa gccgtaaatg gggaacccct
    24481 gtcatgttag gctattttcc cgatacattt ggaaatatgg gacagacgcc tcaattaatg
    24541 aaacaagctg gtatttcagc agcagcgttt ggccgtggtg taaaaccaat tggctttgat
    24601 aatcaagttt tggaagcaga aaactattct tcccaatact cagagatgtg gtggaaagga
    24661 cctgatcaaa cagcaatctt tggtctgcta tttgccaatt ggtatagcaa cgggaatgag
    24721 attccagtag aaaaagaagc agctcttgcc ttttggaagc aaaaattagc ggatgcagaa
    24781 caatatgcct ctacgaacca cttattgatg atgaatggtg ttgatcatca accggtacaa
    24841 aaagatatat caaaagcaat ccatctcgca aatgaactgt ttccagatta cgagtttatt
    24901 cattctaatt ttactgacta tctggaagca gttcaaaaag atgtgccaga agacttaggc
    24961 tcagtagaag gagaattgac aagtcaagaa acggatggct ggtatacttt agccaataca
    25021 gcatctgctc gggtttactt aaaacaatgg aataccaagg tccagcgaca gctagaaaat
    25081 attacagaac cacttgcgac gatggcttat gaagttagtg gaaactatcc ccatgatcaa
    25141 ttagattatg cttggaagac attaatgcaa aatcatcctc acgacagtat ttgcggttgt
    25201 tcagtcgatt ctgtccatcg ggaaatgata cctcgttttg aaaaagcaga tgaagttgga
    25261 aagtatcttg cacaagattc cttagaacag ctgaccgcag caatcgatac gaccggattc
    25321 ccaaaagaca gctttccatt tgtcattgta aatactgcag gaatggataa aactggtgaa
    25381 gcagaaataa ccatcgaatt agaaagaaaa agattcgctg aaggaatacc tgaacaacta
    25441 tatcaagagt tagaaaattt gcctaaacgt aagtatcatg tagaaacaaa aagtggggcc
    25501 actataccag ctattttatc tgaagaaact gtccaatttg gttatgattt gccgaaagac
    25561 cgtttccgtg tgccatatat ggcaagaatg atcaaagtga ctttaccatt agaaaacatg
    25621 ccagcatttt cttgggaaac attcgcgctt gtagaagggg aagcgaaagc agaagaaaag
    25681 gaaacaatga ttcatcagag tggacgaatc atcgaaaacg gccctctaca tttgacgatt
    25741 gaaaaaaatg gtaccattac aatggaagat cgcaaaaaca aacgaaagtt gaataatctg
    25801 catatttttg aagatatcgg cgatatagga aatgagtata tctttaaaca acctttctgt
    25861 gatcagccaa tcctatccag taacaaagaa aactctgaag taaaagtatt agtagataca
    25921 ccggaaatag caaaaataag tattctacag gaaatggaaa tccctgtttc tgcagacgag
    25981 cgtttagaaa aagaacagca gatggttgtg gaatttcgtt atcgaaaagc agaacgatcc
    26041 aaagaaaaac gaatccttca aataaaaaca atcatgacaa tcagaaaaga tagtaaaaaa
    26101 atcgactttg aaacgactct cgacaatcag atgaaagatc atcgcttacg tgtcttgttt
    26161 cctacaaagt tacatgtgga acatcacgaa gcagatagta tttttgaagt ggtgaagcgc
    26221 ccaaaccatg tgtctaaaag ttgggaaaac cccacgaatc cgcaacatca gcaagcattt
    26281 gtcaatattc atgatgaaga gtacggtgta acagtcggaa actttggttt gaatgaatat
    26341 gaagtaacag aagacggaca aatcgcagtg acgctacttc gcagtgtggg agaactaggc
    26401 gattggggat attttccaac accagaagca cagtgtctag gtgagcatcg gttcaattac
    26461 agcattgaac tacatggacc agaagaaaaa ttctctactt atttgcatgc atacgcagca
    26521 cagattcctt tttccactca acaaatcaaa catcatgaag gaacattgat ttcaaaacaa
    26581 caatatttaa cgataaaaag tgaaacgttt gcaattacag ctttgaaacg cagcaagttt
    26641 agtgataaag tagtagtaag aggtttcaat atgtctagtc atttagaaaa attggaaata
    26701 actaaagaca atggaaaaac tgtcatattg aatctgcttg aagaaccaac gaagcaagcg
    26761 gttgtcccaa taatccaacc ttatgagatt cggacaatcg gattcgagga ggaaaacgaa
    26821 tgtatggcgg aatagaagca ggcggaacaa aatttgtgtg tgcggtttca gatggtggag
    26881 aaattttaga aaaattaagt atccctacca caacaccaga agaaacattg gatcaggtat
    26941 ttgaattttt tgatcgttat gaattgaagg gaatcgggat tggctcattc ggcccgattg
    27001 gtatcgacaa aacaaaagat aattacggat acgttttggc aactcccaaa aaagggtggg
    27061 cacaattcga ttttcttggc acaatcaagg aacgctacaa tattcctgct gcttggacaa
    27121 cggatgtcaa tgctgctgca tatggggaat tgaagcaagg agcagcacaa ggaaaaaata
    27181 gttgtattta ccttaccgtg ggaacaggaa tcggtgcagg tgtcgtgatc aatgaagaaa
    27241 ttttttcagg gatcgcacat cctgaaatgg gacatatttg ggtgaaacgc catccagatg
    27301 accattatga aggtacttgt ccttatcata aagattgttt agaaggatta gcagcaggac
    27361 cgtctattga ggcacgcaca gggatcaaag ggcaagatct gccgcaagat catcctgttt
    27421 gggctattca ggcttattat attgctcaag cactgatcaa ctatacgttg acgttagcac
    27481 ctgaaaagat tattttaggc ggtggggtaa tgaatcagga tcatctcctt caaaaaatcc
    27541 gtcagcaatt tgttgaattg atgggtggtt atatggaaac acctcaagta agtgaatata
    27601 tcgttcgttg gggactgccg aatgaaagtg ggattatcgg tagtttgctg ttagcagaac
    27661 acgaacatca aaagtaagca gacaaattac acaaaaagaa cagagtctta tcattatctg
    27721 ctgataagac tctgttcttt tttatttcta ttcccataat ccaggtaaag ctttaccgac
    27781 tattttttct tgagtaaatg gatggatcat gtgcagttct aatgcatgga gcatcagtcg
    27841 ttcagctgtt ctattttggt agagagggtc tccaacgatc ggatgtccct tatgagctaa
    27901 atgaactctg atctggtgtg tacgtccggt ttccaaaaca caatagactt gtgtttgttc
    27961 agaaagtact ttgttgacag tgacatgtgt caaggcgctc tgaccatttt tatcatctac
    28021 gacccttttt ctccgatcat ggcgatctcg tccaatttta ttggtgatgg tcatatcttt
    28081 ggcaatttct ccccatacga tggcttgata tctccgatag atcatttttt gttccaacat
    28141 tcttccaagt atcggcaaga caaatggatt ttttgcaaat aaaacggcac cgcttgtttc
    28201 tttgtccaag cggtggacaa cgtaaggtcg ttgctgttta gtgtcgagat aagcagctaa
    28261 atgattgagt aaggtactgt tttctgtcgg ttcatttgga tgagtcttca ttcctgcagg
    28321 cttatccact ataatcaaat gctcatcttc atataaaaca cggatgtgct tcgcatctcc
    28381 taattgaact tcttgataat tataatctgt ttcttcgaat aacaatgtga tctggtcatt
    28441 gccctttact tcatgatgga acaaagcagg ttgttgattg atccaaacgt ttttacgtat
    28501 acgcaaaaag tgtcgaacct ttctaggaac taaccattcc ttttcaagca agtcgcgaat
    28561 tgttgtttca ggatggttca taggtaaagt gatcgtgatt ttcacaagtg tactcctttc
    28621 agtgcaatcg cagactaatt gtaccatttt taaaacatat ttaagaaaaa acttacattt
    28681 ttctgttcat tcttatgtta ctctacttgc actaacgtaa aaaaacatat tgaactacaa
    28741 cttaagcagg aagaaaacac tctttcacta tggtaaaata agacgattga atcaaataga
    28801 caggagaaga agcaaatgga ttttcaaagc attttagaaa agataaaagc tgctttgatc
    28861 agtttttggc gttggatcag accatatctc ggacaattcc atcgatggcg aaagcgcatc
    28921 tggaaaaaat atcatgtgaa caaattgatt ttgctgattg gtttgatctg tgtcttagtg
    28981 acaagtatct acctctttat tttagcaaaa caagcaaatg tcgaaacgtt aaaatcagga
    29041 ttaagccaat cgacagtcat ttatgataag aaaaacgaag aagctggcac actgtacgcc
    29101 caaaaaggaa catatgtaga attaaacgcg atttctccct atatccaaga tgcagtaatc
    29161 tcgacagaag ataggaattt ttacgagcat cacgggtttg atatcaaagg gattgcccgt
    29221 gcagcagtcc gaatggtctt gtctggcgga acatcagggg gcggaggaag tacgatcact
    29281 cagcagcttg ccaaaaatgc gtatctgaca ttagaccaaa cgtttgatcg aaaagcgaag
    29341 gaattatttt tggcaatcga gatcgaaaag aagtacagca aagaagaaat cttgaccatg
    29401 tatcttaaca atgcctattt tggcaacggg gtatggggtg tccaagacgc ttccagaaag
    29461 tattttggag tagatgcaac gaatgttacg ctgtctgagg ctgctacttt ggcaggaatg
    29521 ctgaaaggtc ctggtattta taatccgatc gatcacccaa aaaatgcgaa tgatcggaaa
    29581 aacacagtac taagtgtaat ggaagaaaac ggaaaaatca caaaagaaca agaacaagca
    29641 gaaagccaga cagatatcag cgcgtatctt aatgacacat acgataattc agattctggt
    29701 tatcgttatc cttattattt tgatgcagtg atcgatgaag cagttaacga gtatggtctt
    29761 gatgaagaag atgtcatgaa taaaggctat aaaatctata cagctttaga tcaggattat
    29821 caaaagcaat tagaagctac ttatcaaaac agtgcattat tcccagcaaa tgcttcagat
    29881 ggtgccatgg tccaatctgc ttcagtagct ttagatccga atacaggtgg tgtacaagca
    29941 ctagtaggac gaagaggaga tcatgtcttt agaggattca gctttgctac acagatgcgt
    30001 cgttcaccag gatcaacgat caaaccatta agcgtatata caccagcact ggaagctgga
    30061 tacaagcctt ccagtatctt gaaagacgaa ccgcaaagtt attacgatgc gcataatttt
    30121 gacgggacat accaaggaga agtaccgatg tatcaagctg ttgcacaaag tttgaatttg
    30181 cctactgtat ggcttttgca tgagatcggt ttacaaaaag gatatgataa agcagaagaa
    30241 ttcggcctgc ctttagcaaa atctgataag tattatggct tagcccttgg tggactggaa
    30301 aaaggagtat ctccattgat catggcgggt gcttacagtg catttgccaa tgatggtcag
    30361 atgtatacgc cgcatcttat cacgaaaatc gttgattcga ctggagcggt gatcgtcgat
    30421 aacacaaaca aaaaaccaaa acaggtcata tcaaaggaaa cagccgatga aatgaccagc
    30481 atgcttcttg gtacattttc aaatggaaca gcggctgctg cgaatcctgc aggttatacg
    30541 atagcaggga aaacaggaac cacagaaact aatttcgatg ccacaaaagc aaatgaccaa
    30601 tggatgatcg gttacacgcc agacgtagtg atttcgacat ggatgggtta cgaagaatcc
    30661 agcaagctgc actatttgga agggacaagt ggaaatgttg tcgggaaagt cttcaaatca
    30721 gcggcagaag gcatccttcc gtatacttca aaaacaggat ttagtgtagc agatgcctat
    30781 gccacaggag gacaagtggt agctgcagat caagttggaa acacaggaaa ttctaatgga
    30841 gagtctggaa attggcaaga taacttgaat caatatggtg aacaagcaaa agaagggctg
    30901 aaaaacttcg gcgatatggt gaaagaaggc gttcagggaa ttggtgaagc ggctaaagac
    30961 ctctggcgga aatatcaagg agagtagccc ctttgaaaac gaataacccc atgttacaat
    31021 agagagtaac tactaaaaga aaagagggca taacatgtca aacatctacg atagcgcgaa
    31081 tcaaatcgaa cgtgagatcc gtgaattacc agaattcaaa gcgttgaaag aagcgtacga
    31141 gaaagtacaa gcaaacgaag aagcacacaa actgttcaaa gacttccaag cgatgcaaat
    31201 cgaattacaa gaaaaacaaa tgagtggaca agagttcagc gacgaagatg caatgaaagc
    31261 acaagaaatg gctgtgaaaa tccaatcaga agaagtaatc aatgacttga tgcaaaaaga
    31321 gcaaggcttc agtacaatca tcaatgactt gaaccgtatc atcatgacac cagttcgcga
    31381 cctttacagc gaataaaact attttatgaa acaacagagg gtgtgaaaga gacgttcggc
    31441 cttgagcgat gacaaaattg cttcttattg ccgataggct gtctcttgaa caccatttac
    31501 caagaggtcg tgagcctacc gtttaactct gagttacaag gaacaatttc aaaaaacagc
    31561 ttctcatgtt tgatgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
    31621 attcagaaat aggagctacg acaaaagtct tgtcatagct cct
//
LOCUS       DACDRN010000026        30009 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDRN010000026 DACDRN010000000
VERSION     DACDRN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.026"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(307..1251)
                     /locus_tag="IWJ80_001428"
     CDS             complement(307..1251)
                     /locus_tag="IWJ80_001428"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9461166.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1547..2491)
                     /locus_tag="IWJ80_001429"
     CDS             complement(1547..2491)
                     /locus_tag="IWJ80_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9461167.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2712..3926
                     /locus_tag="IWJ80_001430"
     CDS             2712..3926
                     /locus_tag="IWJ80_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9461168.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3948..5963
                     /locus_tag="IWJ80_001431"
     CDS             3948..5963
                     /locus_tag="IWJ80_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP9461169.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6046..7419)
                     /locus_tag="IWJ80_001432"
     CDS             complement(6046..7419)
                     /locus_tag="IWJ80_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9461170.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7445..7729)
                     /locus_tag="IWJ80_001433"
     CDS             complement(7445..7729)
                     /locus_tag="IWJ80_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9461171.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7731..9746)
                     /locus_tag="IWJ80_001434"
     CDS             complement(7731..9746)
                     /locus_tag="IWJ80_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9461172.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9925..10230)
                     /locus_tag="IWJ80_001435"
     CDS             complement(9925..10230)
                     /locus_tag="IWJ80_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP9461173.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10319..11350)
                     /locus_tag="IWJ80_001436"
     CDS             complement(10319..11350)
                     /locus_tag="IWJ80_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP9461174.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11557..12432
                     /locus_tag="IWJ80_001437"
     CDS             11557..12432
                     /locus_tag="IWJ80_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9461175.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12584..13426)
                     /locus_tag="IWJ80_001438"
     CDS             complement(12584..13426)
                     /locus_tag="IWJ80_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9461176.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13518..15395)
                     /locus_tag="IWJ80_001439"
     CDS             complement(13518..15395)
                     /locus_tag="IWJ80_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9461177.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15392..16813)
                     /locus_tag="IWJ80_001440"
     CDS             complement(15392..16813)
                     /locus_tag="IWJ80_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9461178.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16816..20205)
                     /locus_tag="IWJ80_001441"
     CDS             complement(16816..20205)
                     /locus_tag="IWJ80_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9461179.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20462..21853
                     /locus_tag="IWJ80_001442"
     CDS             20462..21853
                     /locus_tag="IWJ80_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9461180.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21926..22573)
                     /locus_tag="IWJ80_001443"
     CDS             complement(21926..22573)
                     /locus_tag="IWJ80_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9461181.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22581..23267)
                     /locus_tag="IWJ80_001444"
     CDS             complement(22581..23267)
                     /locus_tag="IWJ80_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9461182.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23273..24346)
                     /locus_tag="IWJ80_001445"
     CDS             complement(23273..24346)
                     /locus_tag="IWJ80_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9461183.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24568..25242
                     /locus_tag="IWJ80_001446"
     CDS             24568..25242
                     /locus_tag="IWJ80_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9461184.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25247..26587
                     /locus_tag="IWJ80_001447"
     CDS             25247..26587
                     /locus_tag="IWJ80_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9461185.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26736..27722)
                     /locus_tag="IWJ80_001448"
     CDS             complement(26736..27722)
                     /locus_tag="IWJ80_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP9461186.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28446..29477
                     /locus_tag="IWJ80_001449"
     CDS             28446..29477
                     /locus_tag="IWJ80_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9461187.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29586..>30009
                     /locus_tag="IWJ80_001450"
     CDS             29586..>30009
                     /locus_tag="IWJ80_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9461188.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
BASE COUNT         8899 a         5619 c         5394 g        10097 t
ORIGIN      
        1 taattgaatg ataaaaacca gtttctttat tcagttaatc aaatatgaac gaattagata
       61 tgaatcattg tatttgccgt tgcggaagga ttttttaatt aaccacagat aaacattgat
      121 aacgattcgc ttattgtaat caacagatta aatttcatat gttcatatgt acggatacat
      181 tttcggaccg attaatttta accattctgt ttatttgctt acctattaga tatcaataaa
      241 aaactgctta acagctattt cagctgttaa gcagttttag gatttttagt tcatattcat
      301 tgtttcttat aatacagtgt tcaatacttc tcttaaagca cttgttgatt tgctgaacat
      361 tgctttttct tcttcgttga ttgataattc aacaacttca cgaacaccat tttcgtcaac
      421 gattgatggc acacctgtaa agatatcttt ttcgtcgtat tcaccagtta aataagcaga
      481 tacaggcaat acagcttgtt cgttattcaa gatagctttt acgatacgtg cagtactcat
      541 accaatacca tagtaagttg cttttttacg atcaatgatc tcataagcag cattacggac
      601 tttatcagca atcacatcta attcatcttg ggcaatacga tggtcttttt ctacaatttc
      661 aaacactggt ttaccaccga cagttgtatg tgaccatgca gcaacttctg aatcaccatg
      721 ttcacctaag atatagccgt ggacgctgcg tgggtcaact tttaatttca atgcgatttc
      781 tttacggaaa cgagttgtat ccaatgttgt tccagtaccg ataacacgtg atgttggcag
      841 accagattcg ttccaagcga tatatgtcaa aatatctact gggtttgaag caacaacgat
      901 aataccgtcg aatcctgatc ccatgatttc ttttacgatt tggcgcatga ttgacgcatt
      961 tgttttaact aaatctaaac gagattgacc aggtttttgg ttgataccag ctgtgataac
     1021 aacgatatca gcatctttac attcttcata gccacctgac catacagcca cattttcatc
     1081 gccccaagcc ataccatcta aaaggtccaa tgcttcccct tctgcttttt cttgattcac
     1141 atcgatcaat actaattcat tagagattcc ttggttgatc attgcataag cgatacttgt
     1201 tccaacaaaa cctgtaccga cgattactac tttacgactt gtttttttca tgaataaaca
     1261 gctccttttc ataactattt gttactttca tgaaaagtat agtcttgttt attcatttta
     1321 tcaacatgtt aattataata gttgttatta taatttttga tggaaacgaa ttcctgaata
     1381 cgcataacag ataaatgaca aatgtttaca cctatatccc ctgataatac aaccaaaagt
     1441 ttagaactga aaataaatcc aaaaaaattc gctgcaacga tataataaga cagcaatcgc
     1501 acctaaaaca attttccttc atatgcttta gtgaaagatt ttcttcttaa tgtttatttc
     1561 tccattcatt gggagtacat gttgaatact tcttgaataa tttgaataaa gaagtcgttt
     1621 gtttatatcc gatacgatta gcgatttctt ccaacgaaaa actcgtttct tgaatcagtt
     1681 ccaacgcaaa gtcgaatttt ttctttagaa ccagttcttg aaaggaagca tcaaaataca
     1741 ttttcaattt attactaaga tagaccggat tgtagccaaa atgagctgct gtttctttta
     1801 acgtacaaga agtaaaatgc ttatcgatat aatctaatac tctaagcatc tcattttctg
     1861 cgcatccatc tgctgatgaa aagctttgtt gttctaataa atgagtcaat tcgatcagta
     1921 atagtgatgt atagagattt aacgaacgga tcttatgact gtatgtatca aaataaatca
     1981 acagcatatt ttcaatcaaa tgcgtcaaac gacttgtttt tccgccgcga aaatgaatga
     2041 attgtgtatg ttcccctttt tcttttgatg catttatcaa aaaagaagaa agtagattat
     2101 ttgtgcttgc caaatgattc aatatctctg tagataaggt ttcttctttt aataagatgt
     2161 tgattaaaat atcctcttgc cctgtgtatt cgattctttg agtagcttct ttatcaatga
     2221 tcaccacgtc actttcttcc atctctatcc tttctccatt aataatttgt aaacatgtac
     2281 cgctatacat ataattgatt tcaacaaaat catgtgtatg ctcgggaact tccgcatatc
     2341 gacggtgttt actgatatga atatttccct gttgaaatag atcccctcgg tggatttttg
     2401 gcggtgtctg aagatggtaa gctaagttta aatcattgat attcattcct tgttcacgct
     2461 gcagagtttc gattgattct tcgttacgca aaaaaaatgt ctaattcagc tagttccaat
     2521 tgactccctc ctttttgtaa gcggtttctt tcgcttatta gttcacgctg tactgctatt
     2581 atatgcaaag ctatttttgg tttcataaag actttctatg gttgggacat gaacttcctg
     2641 tgattccgct attgagagaa taaccacttc atactatatt tttgataagc gcttacaaaa
     2701 atgaaaggaa catgcttatg aataaaaaga tgatgaaatt ttctctgctg tcagtctctc
     2761 ttattttgac ttctgctggt gcagtcacgt ctgcaattcc cagtatgtca gctacttatg
     2821 ccacgcgttc tttatctgct gttgaattat taacgactgc tccctctttt atggtcacta
     2881 tttttgtttt gattagtagt tttattgcta ggaaaatcgg acaaaaacaa actgtgatca
     2941 tcggattagt gattgcatcg atttgtggta tcctccccct cttctcacaa agttacagta
     3001 ttgtcttagc ttcaagagct ggactaggta ttggctttgg attgatcaat tctttggcag
     3061 tcagcatgat tgctgccttt tatcacggag atgaacgagc ctctatgatt ggttttcaaa
     3121 gtgcatttca aggatttggt gcagctttaa tgacatttgt tgcaggacag ctagtacagt
     3181 tcggttggca atataccttt ttagtctacg ccatcagctt acctattctt gtcttgtttg
     3241 ttttgtttgt tcctacacct gaaacaactg aacttcaatc gcatggcaca gccccaacaa
     3301 gacaagtaat caaaacgaat cctaaaatga ttagttatgg tatttttctc ttgctggtca
     3361 tcgtggtata taatgctgta tcgattaaat tagccacagt cctgacgact tattctctag
     3421 gaaacgaaac taatgcagcg aatatcttaa caatcatgca attggcaagt atggctgccg
     3481 gagtttgttt tggctggctt cagacaaaag cacatcgcta tatgcttaca ctttctttac
     3541 tactgatggg aatcggcttt gtattgattg ccactgtgat aaacctgtat gtgattgggt
     3601 ttggtgcgat cctaacagga atttcttttt cgttattcat tccttactta ttcaaccaag
     3661 taagtatcca agcgcccaag tacgcggaga gtttcaatac ttcaatctta ttggttggcg
     3721 ctaatttagg tactttcatt gcaccatatg gtttgctctt tctctctttt gcttctcttg
     3781 ggagtaaaac acttgctgtt ttcgtcaatg gcagtatctt gctgttgatt agcgcgctta
     3841 ttagccttct agtagtacac aggacgaata gaaagacagt gataagagta gatgaagaaa
     3901 taaaagaaca attgacaaca aaataaattt gatggaggtc gtgtaaaatg acaaatgaaa
     3961 aacataaaga tagatggctt tggtatcctg gagactttga gatccgccat ggactgctac
     4021 aaaatttcca acgggaagaa cgaggttttg actggccagc gtactggtac atggatgatt
     4081 gccatcggaa cgtcaagttt aagagatatt actttcttga tcaaccttcc atgtttaaag
     4141 tgacgatcca aggagttggg tatgtagaaa ttaatggaca aaaacatcca tgtggaaaat
     4201 ggctgacttg tccagctggt aaagcaaaaa ttcgtatctt tgtaggacat acatcagggc
     4261 taccagcgat gtttatcgag ggagacgaag taaaatcaga tattggctgg acagcaagta
     4321 attttattga agaatatcca gctggctgga gtccgcttta tgtcgatatc gcaaaagatc
     4381 caaatcaaat ctattatcaa aaagaatgcg tccatccagt cactgaaacg gagattggcg
     4441 gaggaatctt attcgatttt gggcgagcag tgaatggctg tgtagaacta atatctctgg
     4501 atgaccatat cgaatctatc actctatgtt atggagagtc cgatgtagaa gctatggata
     4561 tcgattattg ctactataag caaataggaa tgacaagaaa aatgaaagct cgtaaacgcg
     4621 cttttcgcta tttgtttata ccagaggttt ctactggaat ggttcaactt caagtttttc
     4681 atgaattttt atctctttcg aaacaagctg ctttttcaag cgattcttct ttactgaatc
     4741 agatctggtc agtatcagaa gaagccttca agttatgtag tgatttgttt tttatcgacg
     4801 gtatcaaacg agatcgctgg atttggtctg gagatgcgta tcaatcattc atgatcaatc
     4861 aatatctttt ttttgacgag gaaatcaata aacgaacgat attagcatta agaggacaaa
     4921 atgaaatcaa gcagcatatg aatactatcg ttgactattc gatcttgtgg ttgattggta
     4981 tcgaaaacca ctatatgatg acgaaagact atgaattttt acgcatcgtc taccctaaga
     5041 tgcaatcatt aatgagatac ttgatggagc aaacgaatga attagggttt atttatggga
     5101 gagcaaaaga ttggatattt attgattggt ctgaaatgga taaagaagga acgcttgctg
     5161 cagaacaagt cttattactg aaagcctatc attccatgat cacttgcgga gatatcttag
     5221 ctgaaacata tggagagaac gaaaattatc atactcatct ctatcaagaa aaatatcaac
     5281 tattgaaaca aaacctattt gactactttt gggatgaaga acttggcgct tttatcgatt
     5341 gctatgaatc tgggagaaga aatgtgacgc gtcatgcgaa tattttcgct gttttattcg
     5401 attttgctaa caaagaccaa cagcaatcga tcttaaagaa tgtcttattg aatgacacta
     5461 tcacacagat tactactcca tatttcaaat tctatgaaca agatgcttta tgcaaactcg
     5521 gacagacgaa tatcgtctac caaacgattc ttgattattg gggagggatg ttatctcatg
     5581 atgcggtaac tttctgggaa gaatacgacc ctactcaaac aggaaatgaa aagtacagta
     5641 tgtatggaga tccttatgga aaaagcttat gccatgcatg gggagcaagc ccaatctatc
     5701 ttttaggacg ctattttatc ggtttgtatc caacaaagcc aggttatgaa gaatttatga
     5761 tacaacctaa tctagattca tttgaaacac tgaattgtac gctacctatc aaagatggaa
     5821 agatttcgct tgaaaaaaca aaagataaga tcgtgattac ttctgataga acaggcggaa
     5881 cagtgaaaat gaatggtaaa agttatcctc ttctagcaaa acagccactt gtcttaacaa
     5941 gctattcaaa aataaatgca taaaacaaaa aactgcttgc gacatatcag aagactttaa
     6001 atcttcttct gatattgtcc aagcaatttt tattttgaag tttgcttaat tctgttcttt
     6061 agaaacaatg tttgttcttt ttccggcaaa aagaaagcta gataatacga caagtaaaaa
     6121 gaaagcgatg attccaactg tcaaggctat ttgaccgtat tctcctagtt tcccaagtac
     6181 aagtcctggt actagataat cagtatcggc aaatgtagaa gaagcgattt taagatctcc
     6241 taatactgga aggataaata atggcagcca actaatcaat aagctattga aagcagaacc
     6301 taataccgcc cctcttctac cgccagtcga gttgccgaag acaccagaag cagctccgca
     6361 gaagaaatgt cctactaccc caggaatgat aactgtagtt tgcaagccta tcatagcaaa
     6421 catggatagt gttcctacaa agaagctaac aaaaaagcca attaatacag cattaggtgc
     6481 atatggaaaa acgataggaa catctaatgc tggtttagaa tcgggtacga gttttttagc
     6541 aatcccttgg aatgctggaa cgatttctgc caacaccatt cgaacccctt gaaggacgat
     6601 aacaaaccca gctgtgaatg ttccagcttg gatcagtgca aagattacag cgttgtctcc
     6661 attgcttagt tctctttcaa caaatccagg accagctaac aaagcaagtg cgacatatac
     6721 gatcgacatc aagagcatga tcgatacagt agaatctcgc aaaaagctga aacttttagg
     6781 aatctttatg tcctctgtag aacggtcttt gtttccaaaa aactttccga tccatccact
     6841 agaagcataa ccgatatttc cggtatgccc taacgcaact tgattgtttc cagtgatgtt
     6901 cttcataaac ggttgacaaa gagcaggcgt aatagttaat agtgttcctt cgaagacccc
     6961 gcctaataag atcatccaca aatttgcagt aaaccccgcg cttactaaaa tgactgctgt
     7021 tagacaagaa acataaagca ttgcttgacc tgtaagaaaa atgaatttat atcttgtgaa
     7081 tctagcaagt aaaatattca cgatcatccc tactagcatg atcaaagcag taggtgtacc
     7141 gtattgttgc aaagcaatcg ccactactgc ttcattgcta ggaactactc cttgagtatg
     7201 cagagctgtt tggaacatcg aagcaaaagg ggttagtgaa ttactgatta aaccagcacc
     7261 gccgatcagt actaggaatc cgacaaatgt tttgatagtt ccttggatca cttctgaaaa
     7321 tttcttcttc tgaagaacga gaccaagcag tgcaatcaaa gaaacgagga tggagggctg
     7381 actgacgatt gacacaaaca aattcaacaa tttatccatt gatagttcct cccttgaatc
     7441 atttttattc ttttgacaag atcttcgtta ctttttcttt tacttcttcg ctgtcaatga
     7501 tcgattgcaa aggtacgagc ttttcatcag gtatagaagt tagcgtatct ttcaaagaat
     7561 gctcaatgaa gaaataatca gccatatcgc tagatacact tccaacatcc gtatgactta
     7621 cttcaaactc gtccacatct gctccaagat ctgcaagtgc ttgtttgatg ttcatctcta
     7681 ccataaaact tgttcctaat ccactttgac atactgcaaa aattttcata tcattcgtct
     7741 ccttttctta tgatcgctaa tatttcttct tttgttgatg cgtgtaataa atcttgaaga
     7801 ttttgtttat tagaaagtag ctttgttaaa tttgccaaag cccgaagatg tgcctcatta
     7861 tcgatagcag ctaaacagat gaacaaacta atgggatggg ctggattatc aagcagatcc
     7921 acaggttgtt gaagtttcag caaagacatc cctacttcgt ttacgccatc ttctggacga
     7981 gcatgaggta atgcgatgtg atcgccaata tggataaacg caccatactg ctccacgcgc
     8041 tcgatcatag ctttgatata actttcttct attttatttt ctgataatag cggctgtgcg
     8101 gcaaatgtga ttgcttcttg ccagtttttt ggttgatccg ataattggat catttcttct
     8161 gttaataatt cagatagcat aggacgttga tcctccttct tttctaaaag tttgttcatt
     8221 ttacgattta atacttgata gagcttttct tcgtctacgc cttcttttaa ttcgatataa
     8281 ggcagtagcg ccgacaacaa ttcttgggca gaaggcattg aaaatcctgg tatcagccaa
     8341 tctctttgta cttgattgat cagctgattt ttttccaagt cagtcatgat tggatgcaga
     8401 acatagagag actttttctt atcagtaagt gtgagaggta cagtagaaaa aataatatcg
     8461 taagaagttt cgtcaacctc gtgtaagcgg tcagctgaat tagtctctgt aaaaaagatc
     8521 atcgggaata attgctgaag ttcacttttt aagatcaatg aagagctaat gccacttgga
     8581 cagacgatca cggctcttat ttttctattt ctttcttctg cgtccgcttt tctgatctct
     8641 ccgccaaata gtatagtgaa aaaaccaatc tcttcttcag ggatgctctt ccctattcgt
     8701 ctttctaacg gaagtaatgc ttttcttgtc atttcgtaga tggaagcata ttgatgctta
     8761 atttgatcga tcatcacatt aggtaaataa aaagaatagg aaatacgaaa ataggcaggg
     8821 accaagtgat aaaagacatt catcaataat tcacgaaacg aagcaaattc tacggccatc
     8881 aggaattcca tgcgatggat gacttctgaa gaacattcca gcaaaaaatc aagtgcaggc
     8941 tcctggacat ctccttcaac gatcgatgct aagcaaagag agaaaaatat aatctccgaa
     9001 ttttgatgtt cggaatagcc aaggccagtc aatacttgtc gactaatgtc agtgactgca
     9061 agagaagcca acagttcagc atcttttacc agttccatgg atgtttttcc aattcgaatg
     9121 gttgtcaaac tcaaataata agctaattca tccattcgat ttggtgcgaa tcgaagatct
     9181 gatagtgaaa tcgttttatc tagcaaatct ctaactttgg cgtaataatc aatggaaagg
     9241 gttgcggtta aatacgtgag cccaaatctt ccagcaagtg actgcatcaa acgagcagtg
     9301 cgattttttg cttctcttct taatattgct tctgacccta gcagcctaaa accagttttc
     9361 ctagtatacg acagctgaca atcttccact tctaattcat ttcttatctt tttgatatct
     9421 gacaggactg tccctttaga aacctgaaga aaatcttgaa aatgaaaaac agaaacagac
     9481 gttgcagtcg aataaaccat caaataaaga agatctcttc gctcttgttc ggtatacaca
     9541 gcttcaaatc gagtaatatc caaaaatttt tctgcccagt cgattgctcg gaatttcttt
     9601 atacggatac gggtatcatc cacatattcg gcaaaaggag ttaattgttt gatcgcctcg
     9661 cttgtattta cttcgatgat ctctgctgct ttttctagac tgatttctct tggtgttttc
     9721 aggagataac taattttgta gttcacagcg ctgttcacct cctacttata ttctagtatg
     9781 gtagcgcttt taaatcgagg ggtgtttggt acaacttaca gttggatttt ttttacgact
     9841 gtaccgaacg atttctatcg aatggataca gaaaaaaagc aggtgaccaa atgtctcctg
     9901 cttttatgaa ttgatttcgt ccgatcaatg atgatcatgt ttattttttg aggtcagttc
     9961 ttcaaatttt tctttgacat cttcaaccgc tttttcgata tctttttcaa agtgattggg
    10021 ctcattaggc tttgtttcct cttctacaga atttgaacca gataaagctg tttctttatt
    10081 ttctgtttca ccagagtttg tgccagctaa tgattcttct ttgtcttctg tttcaccaga
    10141 attagttcct gctaaagcca cttcttcttc tgttttaggg gccattgaac ctcttaatcg
    10201 tttgtgtttt tcaccgtcat aatttgtcat gttggttcct cctagtagtt ctatactttt
    10261 tcatcgtaca ataagacagt ttatgcgtca aaaaacaaac ccgattctta caaatatgtc
    10321 atatttttcc ttctgattct tgaatgcttt tgattccttc ttccaaaatt ttcatttgta
    10381 agattgtttc ttcatctttg atgacttttt ctgcgccatt aactaatgtc tcgtaaatcc
    10441 cttcgtagac acggctgtaa tcaccaacct cggaaattac tttttcttca tgataactcc
    10501 cttgttcatc tatataggtc aatacaccat aatgttcggg caaatcgata ccaaagtctt
    10561 catgatcagg catgtaaaac attttcagat gttcttcttg tcgatctttt gtttgtttca
    10621 cgaacatccc tttttttccg taaacgacga aacttggacg ttctttgatg cggaaataac
    10681 tagattttat cgaaattttc atatttccat agaaaaaatc cagatcgaag taatcattca
    10741 ttcgtccttt ccctaaaagt tgacgtacat catagctgat atcatctggt tcgccgaaat
    10801 aagaaagaac ttgatctact gtatgacaag catgtccata taaatagctg ttcgcagtag
    10861 aaaattcatg cgtattttct ggtacttccg gtctgaaata atcataatgc atttctactt
    10921 ctaaaatatc ccctaatttt ccgctttcga ttactttttg gactgtcaag aagtcagaat
    10981 cgaaccgtct gttttggtaa cattggataa atagaccttt ttcttttgct aatgcaaata
    11041 actcttctgc ttcctttgac gtttctgcaa aaggtttctc tactagtaca ttcttaccat
    11101 gttcgagtgc catttttcca tattcataat gaaattgact aggtgtagtg atgacaacta
    11161 attggatctc aggatcatcc caaagatcag acagctgatc agtatattcg atgtcttcga
    11221 atgttttcca cactggttca gatggggaaa aaattttttt gatattgatt ttgttcgtaa
    11281 gttttcttga aaaaggcaaa tgatagcgat ttgtactctt tccattgcca agataagcga
    11341 ttgtaagcat gagtggtgcc tccttttatt gatgttttca gtatatccta gaaaagcgca
    11401 gaaagtattt ataatagcta ttctggtaaa aaagttcaga aaaacacttg ttatcattcg
    11461 gtttggtgaa attaatgttc aaagtaagga taaaaatcaa aaagatagta gcggaaccaa
    11521 gtgtttatca gtaagataga atttggaggt cagatcatgc ttattgaaga aaaactaaca
    11581 aaacaagagt tgtttaccac gacagagaaa cggattgtcg attacatccg taggaatatc
    11641 gaagctgcag tctacatgac gattgaagaa ttagcaaaag ctacgtatac ttcccattca
    11701 gctatcattc gtttatgcaa aaaaatggga ttcagcggat ttaaagaatt ccgtttcgag
    11761 ctggggcggg aagttcatca attgattgct cagttcaatc aaacgattga tcctaatttt
    11821 cctttttcaa gtgaagatag cactcagcaa attgcaaaga aaatggcgga tttatcgatc
    11881 cagtcaataa aaaaagcaca attgcagatt gaacatgaag atttagatca aatcggtaaa
    11941 gtattgaaca aggcacagcg aatctttctt tttgcaaaag gtgattctca aatcacggca
    12001 aggaaattcc agaataaaat ggtcaagttg aataagtttt tgatcatggc tgaagaatac
    12061 agtgattctt cttggaatgc agccaatttg actagcgagg attgcgccat ttttattagc
    12121 tacagcggtc gcatccatca ttatgaacga ataatgacct atttgaagca tgtagaggcc
    12181 cctaccttgc tgattaccgg aaatcagcat tcagaaatgg ccaaacaggc tgatatgtgt
    12241 cttgttatat cgcaggagga atatgatttt gctaaagtag cgactttctc ctctcaaatt
    12301 gccttcgatt acgtattaaa tacgttattc tcagtgatat attctcaaaa ttttgaagag
    12361 aatatccgca acttgaaaaa caaacaagaa ttgatgcaaa aaggccttct taaagaatat
    12421 cggaaaactt aagaaaagat cctcatttag taataagaaa gatagtgttt gataggctct
    12481 ctttgatatc atcataaaaa caggctgaag ttttcttttt caaacctcag cctgtttgtt
    12541 tacggaacga ttcgaataga tatattatag aaatgaggaa tagctaactt ctgtttctta
    12601 ttttgaacga ttgacgaaaa agatatataa gaaggaggaa aatcagtata catgcagcta
    12661 acaatccata caggaaaaat cgtttgttct cggatacttt ctgtacttct ttttgaactt
    12721 cttttgcatg ataaggaacc cggtgccctc tgacaagtaa gcgatgactg ttgaccatat
    12781 aaggtgtaca agttaggagt gtcacgaaat cctgcccttt ttcgattctc agatcttttg
    12841 tatcagtcgg ttctaccgtc ttgatctgat cgacttgata agcgagtgtc cgtccattga
    12901 tttcgatata gaaagaatca cctaaagtca gtttcggtaa gtctgtgaac aactttgctt
    12961 gaggcagtcc tcgatggctg gaaataacgg catgtgtatt ttctcccccc gtaggataag
    13021 acgttccttc caataatgag gcaccttttt ctagcagcac agtattcgtt tcatcaaaaa
    13081 ttggcaaatg gacattgatc tcagggatcg tcaaaatacc gatcgtatgt ttctcaaaat
    13141 aggttctatc ctttttcgca ggttcttttt tcttagtaaa gggatctgca cctggatttc
    13201 caccttcttt tgctaattgc tggtttttct ttgtcatgtt ttcttggatt ttagccatga
    13261 cttcttcatt ttctttgacc gcttgttgct gataatgaga gatgatctgc tgatccagat
    13321 aattattcag cgcgtcgcta acaaatggat aagctagtat ccctattcct gagagaagca
    13381 agaaaagcat cagcagatct agaagtttcc ttttctttgg tttcatttgt gttctccttg
    13441 tgatgactgt ttgataataa aagaaaaagg agcatttgcg taatccgctg ctcctttttc
    13501 caattcttgc aaaaagacta aatctgactg tgcttgcgtc tagcaaagta cagtccagca
    13561 atcagtagaa ggactactcc tgcaccgata tacacataga ttcccttacc gcctgttgaa
    13621 ggaagtgtac ctttgtgttt atttggtatt ttttcaggag aaatcaactg tcctgctgtt
    13681 acataagatt gttcatcgat agtaaatgct acacggtttg ttaatggaac atatttttct
    13741 ggcgctttcg tttcttccag atagtacgtg ccatatttta gacctgtcac atcgattaaa
    13801 ccgttacttg tggttgtaaa aaccgttgct gattctttcg ccgataccca gctgacggct
    13861 tttgtggatg ggtcgatcga taaatatttc gctgtgtcac tatcttgatc acgaacgacg
    13921 aattctgctc cagcaagtgt ttggtctgat gtaacgtcac catctacttt aacaaatcgt
    13981 ttgcccccag taacgacatc gactgacggt ggtgtttgat catttgtatg cccgttatcg
    14041 acatttgctt ggttagaaaa tcctttggtt ggatctgctt tttcgttcaa atgcatatag
    14101 taaacgaatt tcaatgtacc gccaggagtt aatgaaggaa tataattcgg atcaactgaa
    14161 accgtgaatc cgtctgtttg ctcagtgaca gaataattta ctgggtcgat ttctttattt
    14221 ccatcatata aggcataagc atacgttcct gaagaatcat tatcaaatgt taaagcagcg
    14281 tcatgagtat cgataagttt gaatgttgtg tacttgtttt gcgttccttc tttatcagcg
    14341 atacctaatg ggatattgac agaaatctca tattgaattt tttcaccgat tgcgacatct
    14401 tttccattca attgaggtgt tgttttatca actttagacg tatcattttt gatggtcttt
    14461 tctactggtg tttggctatt tgccaggatc tcaaaaggcg tgattgtttg cttttcaatc
    14521 atttccgcat tatcaggagc ttttacttct tctaaattat attttccaac ttctaaatga
    14581 ttaacgatca actggccttt ttcaatagat gcctcggcaa agtcattgtt gccgatgtca
    14641 taagaatgac cagtaatgaa atgtttggct ttggtttgat cagttgtcca agtgtacaat
    14701 ccatctgtga cactttggat gtattttttg acgcttggtg ttccttcttc tttagaaata
    14761 ataaattctg ctccatttag tgcttcgttt tcggcagtac cgatttttgt aactttcaac
    14821 gttccatcat taccgactgt atttttaggg tagagatgga tagtatctag ttcttctgtc
    14881 ccgtatttat aagagccatc tgcttgtttg atcatctcat atacagggaa agctaaaacc
    14941 atgtttgcgg cagctgacac tccgtctttt ggttcttctt tgatcgtgta gactgcatct
    15001 ttcccatttt gttttttagg taaagataaa gtgacatttc catcagcatc tgtcgttcct
    15061 gaagcaactg gtgtaccagg agtcaaagac tggactgctt gttttgctgc atccacggac
    15121 gctcctttat ctcgttgcgc ataaaattct tgagtgacgt tataaactga aaatgaaata
    15181 tcggctaatc cttggtattg atcgaattcg ctcatttctt tcccgctgtt ttggattaaa
    15241 ggatcgggta aatcagtcat tttcttttta tgaaggatga cttgagctgc tgtctcctct
    15301 gcaaacactt tttttgcgcc gaagctgttt gtgagtaatg gtaaaataag gaaaaggact
    15361 cctaacataa cgtttatttt tttatgattt ttcatttttt cactcctgtt ctttttttga
    15421 taaacaacca gattgcaaag cttgcgaata ccataccaac aacgcctaaa agtgtatttt
    15481 tcgtttcgtt tgtttttgga agaaatggac ggctattttc ttttttattg actactttca
    15541 atatactgtc cgcctcttta tcctcagaaa gaaaagttga aatggtaaac ggaatttctg
    15601 tttcacttaa aatataacca gaaggtgctt cgatctcttt taatcgataa cttcctgttt
    15661 tcaacccgga aattgaaaaa gcgccatttt tatcggaaat caggtgaagc gcatctgatt
    15721 cgttctttgt ccacttgtat ccgttggctg tttgctggag gtaatttcct gctttgtttt
    15781 taataaggaa tactgcgcca gctaatcgtt tatctggttt atttgtctcg attttgacaa
    15841 attgcttact ccctgtatat acagagactt tctttgtact gacaccaaaa ccaaaatcca
    15901 gtgttccgtt gttgttgatt tccttgttcg cctgtgctgt actgcttagc tgcatctgat
    15961 aagaaatggt cagttttttt ccagcatatt tttccaacga agggatcgaa aataaaacat
    16021 caaatccgtg aggtttcttg tgtatgacaa agccttctgt cagcttttct ccattcgatg
    16081 aaatcgttaa actattaggt aaaaacgtta atgcttcatc cgcactatct gacaattcga
    16141 acttttgata gtcaaggata tttacaggaa ttgtcgtatg caaggaataa gtgatttttt
    16201 caccaatcgt aaaatcattt ctaggctctt ctaatacctt ctcaaaaggt ggatcagggt
    16261 agtcgttttc ttcattttta ggatacaaat ggatagttga aagtttttga ttgttttgtc
    16321 catgtacagg aagaacaact accatattct ctgccttttc tttgacgact tctggtactt
    16381 tggattcaat gaaaagatac gctttatctc ttttttcttg atctttagcg gccagagaaa
    16441 aagaagcgat accatcttcg ttgttaagag ttgtagttgt tgcttctgcg gtaaacatac
    16501 cggaagacgc accgtttttt gcgatctctg cttgtgcttc ttctaccgtt ttgccctttt
    16561 cccgtagatg gtaaaaagaa tctgtgacat cataaacttg gaatgtgaca ccatttaatc
    16621 ctcgatacgt ttgtaataaa gctttttctt gtccgtcatt tggatgattt ttcggcagtt
    16681 gcccgtttgg gaaaagcaat ttgtgtaaag taatctgaac catttctgtt tctgtttctg
    16741 ttgctacctg agtaaaagct ggtttaaata gtaacaagaa gagacacata ctaagccaac
    16801 caagtttttt catatttagc tgccccctct ttcttttttc aaaaaaagat accaaataga
    16861 aaacgcacaa ccaatcatcc ctactagata gattcctaaa cggccaattc ctcccgtttc
    16921 aggtaatggt acttttgcct gattcgtgat gtctaaagaa atctggttgt ttttggctcc
    16981 tggtgacaga acagattcat gatcttgtcc atccacttga attgacccat cttcgtgtat
    17041 aactatagta actggctctt ttagaccttg gtatccttct ggtgtaaaag tttcggtcag
    17101 cgtataagtt ccaggtttta aattttcgaa tagaaaggta tctgtttctt gtccatcttt
    17161 tggcgattct aagtccattt ctggtccctg cagtctgaat tttgctcctt ttaatgtctg
    17221 tccgttgtct tcttttttgt gtaccgttag atcaaacgct tttagtcgat tttgatgagt
    17281 gagcgtccat acattatttg tcatctcggt attttttccc gtgtaaatga tttttccata
    17341 tttatcgatt tggaattcat aatttcccgg cgaagtatca tatccggcag gaccagctgt
    17401 ttcaacgact cgatatttac caggtgtgag tgaatcaaaa ctggcaagac ctttttcatt
    17461 cgttgtttgg ctgtcaattg gctgataagt accatttgct tcttttttct gtagggaaaa
    17521 tgctgctcct ttaagattta tctctttgtc cgtcccttgc atcgcatact tacgtactgc
    17581 tacaggaact tcaacaaaag gattttcaat agtcaactgg atcgtatcat cggaagtagt
    17641 gactgttttt ccatcaatgg ttaccgtacc atcagaagcg atcttgattt cccaagtagt
    17701 ctttttgctt aatccatgcc cttctggagc ttttgtttct gtcagcgtat aggtcatttc
    17761 tttttgcaat ttgacatttt ctggaagaga ataggttccg tcgccatgat ctgttagtaa
    17821 agtatcaata gaatctcctg ttaatttgaa aacagcgcca ataagatcct tttcacctgt
    17881 agaggaattt ttcgttattt tcaagtttaa cggtgtgaat tgtttagtat ttttccatgt
    17941 cattgcatct atttgttgag aatcgtatcc tggtacaggt aattctttga ttgtttggta
    18001 actgaatgct tgaccttgat tattgtattg aggtagtgat aatatctctt gataactttc
    18061 tccgctattt gcagataatt tgtcaatgtc tgcacgttcc cacgtatttg ttgtatcttt
    18121 agctggttta atgattcgaa tatatccgtt tttccaagct gcagcatttg tcgtatgttc
    18181 ccgttgaatc tcaaaagtaa cttgatctgg acgatcagct agattgttgt caaattcttc
    18241 ccataacttt ttgatgtgaa gactgactcc gggagcttta gcagatggta taccgaattc
    18301 agctaattca ttggtatcaa tgtttggctg gaaagttgtc ctgccgttca tttgatacca
    18361 gaaatttgga tggaagtcct cattttctgt ttggattctc acttggtaat ggatttggat
    18421 ttcttggtct tttcctaaat agatctgatt gctcttgatg gtatttcctt ctatggaaat
    18481 agatggagtg accgttgtag gacttgtccc cacactcttg actgaaagtg taccaggctg
    18541 ataaatgaat ggttctgcga ttggatcatt tatttgtcca ttgcttacag ttgctgagat
    18601 ctgtacagct tttttggcta ggtattcaga gatatctgtt gcatgatcag ctgattcata
    18661 gtaaagatcg cctttttcat ctgatgaaac catttgtcgc atacgagact ctacttctgc
    18721 ttttgagaga ccagctgccg gatcgctttg aagttggatg ccaagaccat gtatttcgat
    18781 tcctcgttct ttgagtgcca tcgcttctcc gatggttgcg ataaacgtac tgtcgattct
    18841 tctggataga ttgttttggt caggtgcatc atagattctt gagattagag aagtatttcc
    18901 cggccgatct tgcgtattag aaaactgagt tccgtaataa ttgctgcttg attgtgcgtg
    18961 tacccgctgt actttatagg aaaatgttgg tacaccatcc gtcagcaaaa cgatcacttt
    19021 tttatgtcca tttggaacgg atagcatgct tcctgcatct cttagtgctt tttgtgtaaa
    19081 agtaccacca tttgtccgtg aaggtgtaat ggattttact tgatttttca ctgaatcaaa
    19141 tgaacccatc tgtactgcac cgttactgta gctatatcct tcgcttgagt agccgacata
    19201 tcccatattg attttgtctg tgataccgct atctgctaaa gtatcgacaa aacgatcgac
    19261 accaatcttt acttcaccga tccgattatt gtcgttcata cttcctgacc agtctacgac
    19321 caatacgaga tcaagaggcg tgatatcttt ttgtacattt ccacgagcat tcaaataaac
    19381 atcaaacaat ccgggtgtac ttgtttcctt tgcatacttt cgtatcgcat agtctgcttg
    19441 attaccggtt cctccatatt cgatatacga atgggtccga tcattaggat ctccattcca
    19501 agaatttata ccatcccatt gatttgttcc gtttttattg ccttgatgat caagaacatt
    19561 tgtatttcct gtaggttgcc aactggcttt aggataagtg ccctgatcat ctgttgtata
    19621 ttctgtagga atcgtttgat tagatggcac actcacagct ggcaaggata atgtaggcaa
    19681 tgtaaatggg ctgacagaat tttcgttttc ttttgatgat tcttcttcta tttcgtctgg
    19741 ctttggtact gacacatctg tatctttctt attttctgtc tcatcaataa tgtcatattc
    19801 tttattatcg acgatcagct tgtttgcctg gctgtggtaa agtgctggat cgattggaaa
    19861 actcagatcg atagtttgtt tagtgtttga tgagatttcc aacgtaattt tcccgctatt
    19921 tacctgataa gcatagccat ctccttcttt tctttccaca tcacttaagt aattttcgac
    19981 tagaccaatt tctttttcta tcttttcagc actggtgtta ttcgcattta gcttgatgtt
    20041 cagtgctttt tttccagact gatccgaaat gacttcagct gaaattgttt ctaaaactgt
    20101 tggatcatct gtagtgtctg ctattgcaga taatggcaca aagtttgata aactcaatat
    20161 tatcagcatg aaaataactt tcagtttctt ccctgtggtc gtcatttttc ccctcctttt
    20221 tctttttttc tgttttgacg tgaatagtga aaattattaa aataaacagg cgttacataa
    20281 ttttatttat tttaatatga taattattat acgaagggcg ctaaagggaa tcacaccaat
    20341 tttcatacca attggtttca tttatgaagt tgattccttc gattaatgag cgtttcattg
    20401 aaagaaaagg gttgagggag aaaaacatat gatcaggttg ttggaatgaa actatagaaa
    20461 gatggaaaaa atcattcgaa ttctgtccat ttctgacaag tggatgacta aaaaagaact
    20521 tgccaaacga attggtagtt cagaatctac atttgtaagg tatatcgaag agatcaaaat
    20581 ccgctgggga acggatgtaa cgattcaatc ttcacaaaaa ttggggtatc gtcttgaaca
    20641 tttgaatgta ccagcttatt taaatgttct gatggaaatg tcgcaaagtt ctacaaacag
    20701 tcgtttatta aatgagatta ttgtcaatcc gggtaaaacg attgagtatt attgtgaaaa
    20761 aatttccatc agccggtctt cttttacacg taagttgaaa caatgcaatc aaattttaga
    20821 aagctattta ttaaaagtga tcgttgacca aggttttcag ttaactagcc ttaaaagtga
    20881 gctccagttg cgtatctttg ttaccttttt ctttttaagt tactacggaa gacgcacgtt
    20941 gccatttcca atcgaaaaaa agaagatcaa agaaattatg gtcaagaata attgtcagat
    21001 gaatttgttt caggaagaaa gcccatatga gcaatctttc tttattatgt attttgttgt
    21061 gcatctcatg agagaatccc aaggaactcc atttgaatgg aaagctggag actcttatgt
    21121 acagttgata aatatcgata aaaaagactt cttaatcttg aaaaatctct taggaaaatt
    21181 gaagtacagc agctatataa aagtagtgaa ctattttgtc aatacgattt tttcagaatt
    21241 ttcggagaaa gaaaaaaatg aaatcagtga gcagatacga aaaaatatgt cctactgttc
    21301 gttttcatcc gctgattgca aagaaaaaaa cgaagaatat gttgttcaat tattaacgca
    21361 aatttatttt ttctcccgta ttttcccata cgatacgact catttgacga tccgtgcaac
    21421 ttactttgca cagcagatgc aacaccgcca gatacgtcta tatgctggat ttcgtgcaga
    21481 acttcaattt tattccactc tactgaatca gaatctgatt tattatttat ctagctatat
    21541 cttttggatg cttatcggta atcctgaaat tcaccattat acttctgata aaaaaatact
    21601 gattatcagc gacttaagtc ttcgacatag tcaatatatt gaagaatata tcagtgatat
    21661 tcttgctgtg cataaaattc actctgagac aactgctatt actgaagatc agctgtcgaa
    21721 atacaatctt ctcgagtatg atctgatcgt tacgaatcaa cccattttga atgcccatgt
    21781 tcctcatata ttgattgatg atagtgtgtc attcgcaaat gaagaagaac tcatccgctt
    21841 attcgagtta tgaataatca aaaaacagct atgtataaac caccctgata aaaatttcag
    21901 gacaaataca catagctgtt tctttttatt gtattccttc agcaatccaa cgagatttat
    21961 agcgaaagtc ttttaacaac tcttcttttg tccaattatt tgcaatgaga tccgtcaaag
    22021 tgttcattac gagggtcatg gaaaagtata ggaaactaac tgcttcttcc gacgattgaa
    22081 tggagaaatg attgattctt aataattcca gccatttttc gttcattgga gaatcagccg
    22141 atagttctct acgtttggtt gtctgctgat catttcgctg ggttaacaag tataaccttt
    22201 gccaatagat actttcgtga tctaagtcgc agcaccactc taaatatttt tcgatcccca
    22261 aaaagaaatg ttgtttgttt tcgctaatga attttaagat atcttgatgg actatgtgag
    22321 aactgctttt gatcgtatac aaataagcat cttccaaatc ttgaaaatat ttataaaaag
    22381 caccgcgcga catccccatc ttgtcaacga tttctgagac tttgacttgg ctgatgtgct
    22441 gtgagcaaaa tatctccagt aataggtcat cgattctctg tttctttgcg ttcgataaat
    22501 tgaaaaaagt ttcttttggc atccacatct ctcctttatc tctgattata cgtggatagc
    22561 ccttcattgt catgaaggat tcattccttc tttttctctt cttctgtcaa gtacccatct
    22621 tccattcgat aaacgcgatc actccattga atcatacgga catcatgagt aaccataata
    22681 gtcgcttttt gtttctcatg agcttcttta actagtaatt tgacgacttc atatgcatga
    22741 tctgtatcca aactagctgt tggttcgtct gctaagatca agcttggctc attatataaa
    22801 gcgcgagcaa ttgccactct ctgtctttct ccaccagaaa ggtctttagg ataactattt
    22861 ttaagtgact ggatatctaa agattgcagc aaggaatcga tttttgttga atcttctttt
    22921 tttgaagtaa cttggtcaac tagttcaaat tgttccttta ccgttaaaaa aggaatcaaa
    22981 ttagagcttt ggagaataaa tcctatttct ttaaagcgta aatctgctcg ttttttttct
    23041 ggaagctcag ttagtgagtg accgttgatc actatttctc cggaagtcgg tgtttgcaat
    23101 cctcctgaaa tggtaaggaa agtactttta cctgaaccag aaggaccaat gatgctgacg
    23161 aattctcctt cctttacata aaaatcaatt gctttcaaag ctgagacctt tgtatgacca
    23221 gaaccaaatg ttttggtaac ttttttcatc gttaatattt ttttcatttt tattaacctc
    23281 ctattgctgt aattgggtct actttggtta tcgtacgtat cgaaaacaat ccgcctagca
    23341 ttgcgacaat gatcaaaatc ccactatata gcaaccattg tgaccaaaaa acagcaaaag
    23401 gcatcgcgct tggtaaaatc agactagtca aataagcaaa caaaagagca agcagaacgc
    23461 ctaccacccc aacaataaag gactgtgcta ctaaagattt agcgatgaag aaatttttga
    23521 tcccttgtgc tttcattact ccaaaaatag cagtcttctg aagtgtcatc acgtacatga
    23581 aaataccgac cacggcagca accactaaga acaaaaagta tatcatcgca tttaaggtca
    23641 tattttgggc agaatagcca ggaatacttt caatgaatgt cccaatagtt aataactgta
    23701 gacccttcga tgctttttct ttcgaaattt tagctgaatc ctttattacg atcccattga
    23761 tcggctgttc ttcttcagaa gcaaacggct gttgtccata cttcaaagca gtccacgtat
    23821 ctagggacgc atagatgacg ggagaaactg tgtaataagt ttcaggaaag ataccgacaa
    23881 tcgtcaaagg atcgtcgtaa ctgccaattt tgatcgtatc tccaatttta tagcctgaat
    23941 cagccagatt ttgagagatg atgatttcat tctttgtttt aaattcatgc cctttcgtga
    24001 gttttggtaa taaaaatgct tctttcgttg tgccgaaaac tgtaacattt tctttgtttt
    24061 ttcctttgat tgctccgctg tacaatccga ttggtgcttt ctctcctcct tcaatgtatt
    24121 tcagatctcc tcttgttaat tgtgaagctg caaatacttg atttgcatcc tccgacaaaa
    24181 caatctcttg tgcgtcccaa tcatcgattg cttttttaaa ctcttctgaa agtccattcg
    24241 ctagtcctga caacatgaat acgacatacg caatcaagaa cataataccg acgatcaagc
    24301 catatcgtaa tttagaatag cgaatttctt tgatagctag aaacatttgt ttttcctccc
    24361 tatgatagat tgatagtgac aaaatgtcac ttgttgaata gtgtaactct aaagtgacaa
    24421 tttgtcactt gtgttgtttt tttcttatca ataaattgtc taagacatct ttcgataaaa
    24481 ccttaacatg agtcataaaa aatttattta agccttattt atgctttcca tgataaacta
    24541 aagtgaccaa atcaatgagg tgggaaaatg attaaactat tagtagtaga agacgataaa
    24601 gtattatcca ataatataaa agaaatactc gctgcaatcg gcgagataac acaagtttat
    24661 gatggtagtg aaggactgtt cgagttggaa agtggagttt atgatcttgc aatcttggat
    24721 ttgatgatgc cggaaatgaa tggatatgaa gtgttagcaa aaatacggaa agaaaaaata
    24781 cagactccag tattgatttt gactgcaaaa gatagtttgg aagataagat tgaaggattt
    24841 gaacgaggtg cagatgatta tctaacaaaa cctttctatc gagaagaact gatcatgcgc
    24901 gtgaaagcat tattgaaacg ctcgctaggt ctctttgatg aaaatgttct gaagtataag
    24961 gatatcagtt gtgatttaaa aagcaatgaa gtcttgtata aagaacaagt tttgcccata
    25021 caaggaaaag agtttgaatt gctcgtttat tttttacaaa ataagggaat cattttgaca
    25081 aaagagcaga tatttgaccg gatctgggga tttgattctg atacgacgat tacagttatt
    25141 gaagtttata tgagcaactt acgaaaacat ctgcgaagta ctggattgga taaagaaatc
    25201 aaaaccttac gtaatgtcgg ttatatactg caaagggtgt gatgcaatga atggagatat
    25261 tacacgaaag caacgtagtc gcttttttgc gacaaatgta gcagcatttg cttttatctt
    25321 ttttcttttg ggattcatta ctttacagat attaaaccaa tctgcataca gagaaacaga
    25381 tctgtcatta gagaaaataa attttgattc tcgaacagta caattagaga tacaaagata
    25441 tcagcaaggc gatccctttt taaacccgac tcaaacggaa caaaacagaa caaacatgga
    25501 acagccatta atggaaccat caaatgaccg ttttaacact caagtgatct tgtggtctaa
    25561 aaatggtgag atattgaata aagcagctat tggtgggcgt ttcaaccaaa tcaaagaatt
    25621 aaaaatggat acgaaaaatc tgaataccat ccaaaatatt gaattaaatg aagacagtgg
    25681 ctcatctgcc ctctctttcc gttcgattac gaaaaaagcg gagacgaatc aagatggcgt
    25741 tgcctacatc caaattttag aaaatacaaa tcaaatcaag aattcattag cgacttttcg
    25801 cacgattatg attttgtgta tgattgtttt ttggattctc tctattggta tcagttatta
    25861 cttgtctagt ctaagcatgc gccctattat tctttcttgg agaagacaaa aagaatttgt
    25921 tgagaatgct tctcatgaat tgcgcacacc gttaacgatt attcaaaata gtttggaaca
    25981 tctttttacc aagcctaatc atactattat tgaagaatcc gaaagcatcg ctcaagcatt
    26041 gtcagagacg aggcgcctca ctggattgac ttcggatttg ctgacaatcg ctcggaatga
    26101 ttctgatcag cagctgcttt caaagcaaat gatcaataca caagaatata tcgaacaact
    26161 tgttaaacca tttcaagaga tggccatcat agatggaaaa gagtttattc tccataacga
    26221 tgcaacatta gaagtaccaa ttgatgaaaa aaaaatccat caagttttgg ttattttgct
    26281 ggataatgca ttaaaatata cgaatccaga agataagatc attttggatt catctgttaa
    26341 ccataaagaa tggctgattc acgtaaagaa tactggccct tctatcagtg aagaagatca
    26401 agcaaggatt tttgatcgat tttatcggga agatcaatct cgggcgaaag agactggcgg
    26461 atacgggctt ggcttggcaa ttgcaaaaca ggtaatcgaa cagcatcatg gacagatttc
    26521 tgtatccaat tatcttccac aaggagtcga ttttaaagtc caattgccgt tgaagcctaa
    26581 aaaataatgc ttatccctaa aaaagggctg tgacaaaaat cttgtcacag cccttttttg
    26641 ttgcgaataa acggtgttcc agaagtagct tcttcggaaa taagccgaaa tttctttaag
    26701 ctgaccactt ctgtcacaac ctctttagct tacgcttacg attttttatt gcggtcttca
    26761 atgtgtacat caccgcttgt gtcaatatcc aattcttctc ggctaacatc ttctgtcact
    26821 ttttcaacat cttcatgttc ttctttatga atcccaactt cttcacgaat aactgtatgt
    26881 ttgcttacat ctacttgctc ttctttgatt gggatagtaa tcgtgtcatc ttccaatttt
    26941 tcgtctgttc cctgtgaaga ttgatctgtt actggtttgc gttcgatgac gatttcttca
    27001 cgtttgaccg gtacttctac cgtttcagta tcgttgacca catgtttgtg gatgtcgact
    27061 tctccagttg tcacatcatg tgtatttaca tctaattttt cttcttttag ttgaattgta
    27121 tcatcatcta gatttggttt gcgatcgatg tcttcagtct tgcttgtacc cattgtatca
    27181 gtctcgccag gtacgtccat cggatcagtt cctggtgtca tattcaatgg atccgttgta
    27241 ttttgaaagc ctgtcgtgcc acctacgtcc attggatctg ttccagtgcc agtttctaat
    27301 ccagtcccca ttgtgcttgc tgggctaaca gtatcttgtg tatccattgt atcttctgtt
    27361 gtttctggct gacgatagcc ttttactgtg atgacaaatt ttccatcaga aatatctcct
    27421 ctatattgat ataaaggatc attttctggt gtagccgtct cagaatcata ggtatcaaaa
    27481 gaaaaagtat cttttatttt ttcccataaa gaacggtctt cttcaccatc catccgattt
    27541 tcatctgttt caacatctat accagacaat ccgtcaaatc ctagtctaga tgctgcttca
    27601 tcagtagtat aaataacgat atcatctcgt tcataccctt cgttacgcaa ccgttcaatt
    27661 actgataatg cttcttccct cgtactatag cttccgatga ccgttaaatt tttctcttcc
    27721 atgtaaattc ctcctagcaa atagatttat gattggtata ttgttactta taaatttatt
    27781 ctataggaga cgattgtttt ctacaaatat ttagattgga atttgattaa aacaatttaa
    27841 ttgaaatttg aaaacattta tgttaatgaa agtcatattc taataaaatg tcatttgttt
    27901 ctatatgaat ttttatgaaa aatttcatat aatttccact cgttgtgatg gtttaattta
    27961 tccaatccat caataagcaa acaacttata taaaaaagga ttaattctca atataattgc
    28021 tcctaccata gaataaatct tcatgtaaag taatgaagta ccaactattt ctaatacttc
    28081 tttaagaatt atcccaacaa tatcgttaga caaaccatta ttgaataaat ggagagccaa
    28141 tcgattctga aaaaatgttt tctaattttt cggaaataaa agaaagagcc tcctgccatt
    28201 agaacgaacc aaatccattt cattgctcat gaagtaacca catagctaaa aagaggataa
    28261 atcaattaaa agatcaaaag aatgactgca ctaataaaat aactaaaaat gtatcttctt
    28321 acttcatatc ttttagctag tacttagtta aaaatttgct tcctaagatc aagacaaact
    28381 gtctatcttc atttgatgaa atgcggtaaa atatagaaaa tgaacactat tggagggaat
    28441 ttgtaatgga cagcaaaact agaaaacaac tgcaagaaaa gttaaagcaa cgtacacact
    28501 tgctcccttc cgtctttcaa cagtttttag aagaaaatcg ttcttccctt tcattaggca
    28561 gtcgctatga atacgcaaaa gattttcatc tgtttactga ttatttgaga aaagtatttt
    28621 ctgaagaagt ggatgatcag ctgattatcc atttagaaaa acaagattat cagaatttct
    28681 tagctaaaat ctatcgacat actcgttcgt tccaaactac agcgaaccaa gaaacggagc
    28741 aattgtttga aaatagctat tacgggattt caaggaagca atattcatta aatcattttt
    28801 tacgattttt gtatcaaaaa ggactgattg aagaagagat ttctcttttg ggtacaagtt
    28861 taccggaaac aaccaaagca gctccgcgat atatcgatgc agaacttttc aaagattttg
    28921 agcatttgtt tatcccgatt gaaggctttc aaacaagcag agaagctagc tttgaagaaa
    28981 agaatagacc gaggaacctt gcaattactg ccattcttct gtattgcgga ttaaagatcc
    29041 atgaagtagt agaacttaaa cgtaaagatg tatctttgac caagcagatt cttaatctga
    29101 atagaaaaga aaacagactg aataaagtac caataccaaa agaagcgatg ccatttctgg
    29161 agaactattt taagttaaca gcacaaaata cggacgaaaa ttcttttgtt tttcgttcct
    29221 cacatgatca gcaaatttct cctcggacta ttcgccaaat tttgagcaaa attacagtat
    29281 ataaaaacgt atcgccagaa ctatgcagaa aaacatatcg ttattatgca gaactatatt
    29341 tagatgatgc tgacgcggta aattatttat gtggtaatcg atacctctca tcccattctt
    29401 ttcaagatat ctgggaaaag atggttgatt tttcttatca cgaattagcg gcattcgttc
    29461 gggtttcttc catataaaac aaatgtatga tctttttttc acaataaact atgttattat
    29521 ataaaggatt atgttacgat tatcttgaat ttgtatatta cttaacaaaa gataatggag
    29581 tgtgaatgat ggagaccata acgtattccc ctttgaactt atttacgaat tttcaagaag
    29641 ctgcagctaa cttttccgag accccgatta tctttgatga gccactttca gctttcccag
    29701 aattaggact tgaaacaact tacaaaaaat gctgcgaaag tgtgttgaaa cgggcttatc
    29761 aattggctca tcttggagtg aaatccggag acaaagttat tatttataag agttccgctt
    29821 ttgacacata ccttctagct gtttctgtgt cttaccttgg tgcagtgcct gtcatgactt
    29881 cttatcatct accaacaaca acaatggaag tatttatcga tcgtttagaa gaccctttca
    29941 ttttatttga tgatgagaca aaaaagcgag tgagagaaat ttcaaatgga acaaaatcaa
    30001 aacagattc
//
LOCUS       DACDRN010000027        29946 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDRN010000027 DACDRN010000000
VERSION     DACDRN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.027"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(26..328)
                     /locus_tag="IWJ80_001451"
     CDS             complement(26..328)
                     /locus_tag="IWJ80_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9461189.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="IWJ80_001452"
     CDS             complement(353..2446)
                     /locus_tag="IWJ80_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9461190.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="IWJ80_001453"
     CDS             complement(2935..3699)
                     /locus_tag="IWJ80_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP9461191.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IWJ80_001454"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IWJ80_001454"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP9461192.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="IWJ80_001455"
     CDS             complement(4704..5780)
                     /locus_tag="IWJ80_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP9461193.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="IWJ80_001456"
     CDS             complement(5804..6397)
                     /locus_tag="IWJ80_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP9461194.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IWJ80_001457"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IWJ80_001457"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP9461195.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="IWJ80_001458"
     CDS             complement(8067..8366)
                     /locus_tag="IWJ80_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP9461196.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IWJ80_001459"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IWJ80_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP9461197.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="IWJ80_001460"
     CDS             complement(10121..11032)
                     /locus_tag="IWJ80_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9461198.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="IWJ80_001461"
     CDS             11152..12009
                     /locus_tag="IWJ80_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9461199.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="IWJ80_001462"
     CDS             complement(12196..12558)
                     /locus_tag="IWJ80_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461200.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12904..14343
                     /locus_tag="IWJ80_001463"
     CDS             12904..14343
                     /locus_tag="IWJ80_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9461201.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="IWJ80_001464"
     CDS             complement(14350..16902)
                     /locus_tag="IWJ80_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP9461202.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="IWJ80_001465"
     CDS             complement(17016..18347)
                     /locus_tag="IWJ80_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9461203.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="IWJ80_001466"
     CDS             complement(18313..19791)
                     /locus_tag="IWJ80_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9461204.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="IWJ80_001467"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="IWJ80_001467"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9461205.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22401..23828
                     /locus_tag="IWJ80_001468"
     CDS             22401..23828
                     /locus_tag="IWJ80_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9461206.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23920..24285)
                     /locus_tag="IWJ80_001469"
     CDS             complement(23920..24285)
                     /locus_tag="IWJ80_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9461207.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24282..24647)
                     /locus_tag="IWJ80_001470"
     CDS             complement(24282..24647)
                     /locus_tag="IWJ80_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9461208.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24839..25087)
                     /locus_tag="IWJ80_001471"
     CDS             complement(24839..25087)
                     /locus_tag="IWJ80_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461209.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25086..25214
                     /locus_tag="IWJ80_001472"
     CDS             25086..25214
                     /locus_tag="IWJ80_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP9461210.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25271..26965
                     /locus_tag="IWJ80_001473"
     CDS             25271..26965
                     /locus_tag="IWJ80_001473"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP9461211.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            27032..27238
                     /locus_tag="IWJ80_001474"
     CDS             27032..27238
                     /locus_tag="IWJ80_001474"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461212.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            27616..28749
                     /locus_tag="IWJ80_001475"
     CDS             27616..28749
                     /locus_tag="IWJ80_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP9461213.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28780..29100
                     /locus_tag="IWJ80_001476"
     CDS             28780..29100
                     /locus_tag="IWJ80_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461214.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29129..29593)
                     /locus_tag="IWJ80_001477"
     CDS             complement(29129..29593)
                     /locus_tag="IWJ80_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9461215.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29725..>29946)
                     /locus_tag="IWJ80_001478"
     CDS             complement(29725..>29946)
                     /locus_tag="IWJ80_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9461216.1"
                     /translation="VSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAA
                     VNLVLSILIYLPFVKVATIQERRKEALDA"
BASE COUNT         9032 a         6212 c         5169 g         9533 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattactta ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    22021 attttttttt tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg
    22081 gatttttctg ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa
    22141 aatagataac ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca ttcactaaac
    22201 atatttttat ttttttatat tgttaaaaat tcgcaattta ttttttaata gaattatctg
    22261 ttttccaaat tttataacat aaaaaacctt ttttaacggc atttatatgc tttttcagaa
    22321 attaactgat gatttcaaaa agaaaacatg tcataatatt tcccatattg tgcaaaaaaa
    22381 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
    22441 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
    22501 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
    22561 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
    22621 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
    22681 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
    22741 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
    22801 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
    22861 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
    22921 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
    22981 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
    23041 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
    23101 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
    23161 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
    23221 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
    23281 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
    23341 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
    23401 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
    23461 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
    23521 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
    23581 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
    23641 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
    23701 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
    23761 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
    23821 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
    23881 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
    23941 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
    24001 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
    24061 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
    24121 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
    24181 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
    24241 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
    24301 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
    24361 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
    24421 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
    24481 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
    24541 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
    24601 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
    24661 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
    24721 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
    24781 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
    24841 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
    24901 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
    24961 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
    25021 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
    25081 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
    25141 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
    25201 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
    25261 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
    25321 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
    25381 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
    25441 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
    25501 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
    25561 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
    25621 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
    25681 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
    25741 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
    25801 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
    25861 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
    25921 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
    25981 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
    26041 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
    26101 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
    26161 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
    26221 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
    26281 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
    26341 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
    26401 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt
    26461 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
    26521 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
    26581 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
    26641 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
    26701 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
    26761 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
    26821 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
    26881 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
    26941 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
    27001 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
    27061 gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca
    27121 agtaataaga gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt
    27181 accgcagatg caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt
    27241 tcgagtatga agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt
    27301 aataccgaga aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt
    27361 gaaaacaact attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac
    27421 aactatttca atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa
    27481 tacataagac tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa
    27541 gatctccttt aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg
    27601 aaaggagcat ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga
    27661 aacgtagatg atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca
    27721 gccatttgcg gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg
    27781 tatgttgtag gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca
    27841 ttgaaaaaag ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc
    27901 tgcctgaatc acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg
    27961 cttttcggat ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga
    28021 gttccttatg cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa
    28081 gttttatttc tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta
    28141 aaaaaaggcg atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa
    28201 ttcgctgtta tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta
    28261 gataaagcgg ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct
    28321 ggaacagaat tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga
    28381 atggacggat tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc
    28441 acaatctctc cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta
    28501 actggtgttt atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat
    28561 atcgctgtca agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg
    28621 attgcagaag gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa
    28681 gcagcacaag catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg
    28741 aaaccttgaa ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat
    28801 acccatgaga tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc
    28861 ttctatttcc aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt
    28921 ttctatattt ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat
    28981 ttttatttgg ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac
    29041 atttttattt acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga
    29101 gaatttttca aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag
    29161 cggtcgtccc actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa
    29221 acgtggtatg atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc
    29281 tgatttccgc taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca
    29341 gggcacaaag atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg
    29401 ctcctccgac gatggtttct ttttcatcta agaccacata ccattgagga atactttcct
    29461 tattagcgat tccagtacga atactttcct catacacaac agcaggaact ccccattttg
    29521 atgaaaacca attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt
    29581 tatatgtaac caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag
    29641 tctttatgaa aattagcaat gacaacaata gtgatgtaaa aaaaaaaacg gaaaacaatc
    29701 acttgttctc cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat
    29761 cgtagctacc ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc
    29821 tagtacacca cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac
    29881 ccatggaggc ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac
    29941 actaac
//
LOCUS       DACDRN010000028        28558 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDRN010000028 DACDRN010000000
VERSION     DACDRN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.028"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(5..307)
                     /locus_tag="IWJ80_001479"
     CDS             complement(5..307)
                     /locus_tag="IWJ80_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461217.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(556..756)
                     /locus_tag="IWJ80_001480"
     CDS             complement(556..756)
                     /locus_tag="IWJ80_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9461218.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(1061..2293)
                     /locus_tag="IWJ80_001481"
     CDS             complement(1061..2293)
                     /locus_tag="IWJ80_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9461219.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2547..3116
                     /locus_tag="IWJ80_001482"
     CDS             2547..3116
                     /locus_tag="IWJ80_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9461220.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3294..3734)
                     /locus_tag="IWJ80_001483"
     CDS             complement(3294..3734)
                     /locus_tag="IWJ80_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP9461221.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3892..4656
                     /gene="map"
                     /locus_tag="IWJ80_001484"
     CDS             3892..4656
                     /gene="map"
                     /locus_tag="IWJ80_001484"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP9461222.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4688..5611
                     /locus_tag="IWJ80_001485"
     CDS             4688..5611
                     /locus_tag="IWJ80_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP9461223.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5687..6826
                     /locus_tag="IWJ80_001486"
     CDS             5687..6826
                     /locus_tag="IWJ80_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP9461224.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6819..7619
                     /locus_tag="IWJ80_001487"
     CDS             6819..7619
                     /locus_tag="IWJ80_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9461225.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7619..8446
                     /locus_tag="IWJ80_001488"
     CDS             7619..8446
                     /locus_tag="IWJ80_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9461226.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8424..9158
                     /locus_tag="IWJ80_001489"
     CDS             8424..9158
                     /locus_tag="IWJ80_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9461227.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDKLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            9258..10124
                     /gene="rfbA"
                     /locus_tag="IWJ80_001490"
     CDS             9258..10124
                     /gene="rfbA"
                     /locus_tag="IWJ80_001490"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP9461228.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            10138..10710
                     /gene="rfbC"
                     /locus_tag="IWJ80_001491"
     CDS             10138..10710
                     /gene="rfbC"
                     /locus_tag="IWJ80_001491"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP9461229.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10732..11760
                     /gene="rfbB"
                     /locus_tag="IWJ80_001492"
     CDS             10732..11760
                     /gene="rfbB"
                     /locus_tag="IWJ80_001492"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP9461230.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11858..12709
                     /gene="rfbD"
                     /locus_tag="IWJ80_001493"
     CDS             11858..12709
                     /gene="rfbD"
                     /locus_tag="IWJ80_001493"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP9461231.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12744..14777
                     /locus_tag="IWJ80_001494"
     CDS             12744..14777
                     /locus_tag="IWJ80_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461232.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14821..16101
                     /locus_tag="IWJ80_001495"
     CDS             14821..16101
                     /locus_tag="IWJ80_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP9461233.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYRWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16311..17117
                     /locus_tag="IWJ80_001496"
     CDS             16311..17117
                     /locus_tag="IWJ80_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9461234.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            17129..18349
                     /locus_tag="IWJ80_001497"
     CDS             17129..18349
                     /locus_tag="IWJ80_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9461235.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18339..19925
                     /locus_tag="IWJ80_001498"
     CDS             18339..19925
                     /locus_tag="IWJ80_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9461236.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19964..22102
                     /locus_tag="IWJ80_001499"
     CDS             19964..22102
                     /locus_tag="IWJ80_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9461237.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(22170..23129)
                     /locus_tag="IWJ80_001500"
     CDS             complement(22170..23129)
                     /locus_tag="IWJ80_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP9461238.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            23464..24855
                     /locus_tag="IWJ80_001501"
     CDS             23464..24855
                     /locus_tag="IWJ80_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9461239.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAEIDFSQEKIYR"
     gene            24914..25717
                     /locus_tag="IWJ80_001502"
     CDS             24914..25717
                     /locus_tag="IWJ80_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9461240.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGKRIVPEYNNAIRVFSKRRNPFNHMTVMFD
                     KKAILEVGNYQPLSGFEDYYLWARLLKAGYKGYNIQETLVYARTGKDMYARRGGMKYL
                     GSGLMGRYRIWKDGLGSVKDFIIVSCGHIFISLLPNQLRGKFYQTQLRK"
     gene            25750..26604
                     /locus_tag="IWJ80_001503"
     CDS             25750..26604
                     /locus_tag="IWJ80_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HAP9461241.1"
                     /translation="MKKIQQITLKMSQEIVDFCLKNQLLCYFCGGGAIGTVREQGFIP
                     WDDDLDFFMPREDYEKFYQLWVNSEKSKKYPIQKASKNYNDHNSFTTIRDADTTFIKT
                     YQRDLDIVHGITIDIFPLDVAPVSKFSRKIQKLWALVYALFCSQVVPEKHGGIMATGS
                     KILLSIFRSPRTRYKIWSFAEKQMTKYNNEPTGYVTELCVGPKYMGNVYPAKDFESAV
                     WVPFEDTEMPVPVGYDNYLSQVFGDYMQLPPEKDQVSHHEAVYIDPEHPYTMYKGKYY
                     LTKGAEKK"
     gene            26601..27302
                     /locus_tag="IWJ80_001504"
     CDS             26601..27302
                     /locus_tag="IWJ80_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAP9461242.1"
                     /translation="MITAIIIAGGVGKRMGQDIPKQFITIEGKPIIIYTLESFQHHPQ
                     VDRILVVCKSGWEETMWAYIKEFNISKVKWVITGGSKGQESINNGVQFLKDYSEDDDT
                     IIIHDGIRPLVDELVLSDVIVKCKEFGNGVTSLPYNEQIFIKKTEETTEQYVDRNTLR
                     RVSTPQAYQYGKLRAAYDRAVKEDIGMTDSSYTNTMMVDLGETLYFAAGSDKNIKLTT
                     TDDLELFKAYLKMKD"
     gene            27310..28377
                     /locus_tag="IWJ80_001505"
     CDS             27310..28377
                     /locus_tag="IWJ80_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9461243.1"
                     /translation="MMTLIKQPMYQEDLAKVINHTPKIKQLHGKSVLMIGASGMIGSF
                     LIDTLMAANEQLGIKIKVYAMGRNRQKLEKRFASYLELDTFEIVEGDVTEPLPKEIQA
                     DYLIHGASNTHPKAYATDPIGTIMTNLAGTEQVLKHAVATQPVRVLFLSTVEIYGENR
                     GDIEKFTEDYCGYIDCNTLRAGYPEGKRVSESLCQAYIAKHDLDIVIPRLCRTFGPTM
                     LLSDTKASSQFILNAVEQKDIVLKSEGNQYFSYVYVGDAVSAILHLLLEGEKGEAYNV
                     SSENFDLRLKELAQQLADISGKQVIFELPDEIEQKGFSKASTAVLENKKIKETGWKPI
                     FPLNESLVHTVELVKGEKYNV"
     gene            28370..>28558
                     /locus_tag="IWJ80_001506"
     CDS             28370..>28558
                     /locus_tag="IWJ80_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9461244.1"
                     /translation="MCEISIIVPVYKVEKYLRKCVDSILAQTFTDFEVILVDDGSPDN
                     SGKICDEYAEKDNRVRVIH"
BASE COUNT         9166 a         4749 c         5809 g         8834 t
ORIGIN      
        1 ttatttaatc tttgtatctt tctggatcaa cgaaagtata ctttatatag tcataacgcc
       61 gatgatcgct acgtgcgtcc ggcacgtcag tcacgatatc aaacaaaaag tatacaccct
      121 tcttcattct agttttcgca gcaggaattt tgaaatagtt cttattagaa tagtagagat
      181 tgattaataa gctatcttcg attgctaaaa agaaaacttc tgaattccat actttataaa
      241 aatctttgat aaatctattc gaagggtcaa atttaaacca taattgtgtt tttccttcca
      301 tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt gtgaaggcgg
      361 aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg ccaaaaaaat
      421 tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt cctgcaataa
      481 caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc cctgtatcct
      541 ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac tgctatgtcc
      601 taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag atgcatgcgt
      661 atgtcttagt ccatcaataa acagtcgaag agaaaatagg gaaaaattga ttactaaaga
      721 aaaacttact aggtcgacgg tgtagttgat tttcatactt caatagattc gaattaaacg
      781 atttcgattg gcttatacca gtcctatcca acgaaagata tatcgttctt aactctagcg
      841 gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac tttttattat
      901 ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta ccatataaaa
      961 aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga tcctttattc
     1021 tatgctttat attatttttt gcatgtgtgc gaaagtatac tcaagcccaa tcgccatttc
     1081 ggaaaatcgg tactgtagag ccatcttcac ggattccgtc gatatccatc ttgtcagatc
     1141 ctaccatgaa atctacgtgg gtttgacttc tattcaatcc agcttctgct aattcttcat
     1201 cggacatttc tgtaccgcct ttcacactga aggcataagc agaacctagt gctaagtgat
     1261 tcgatgcgtt ttcatcaaat aatgtattga aaaagatgat gccagattga gaaataggtg
     1321 aagggtcagg tacaagtgcg acttctccta aacgacgcgc accttcatct gtatcaagca
     1381 gttttgctaa gacatcttct ccttgttcgg cagaaaaatc aacaactttt ccatctttaa
     1441 aagtaaattt catacccgag atgatggtgc cagcataact cagtggcttt gtacttgaaa
     1501 tgtaaccatc tactcgatga ctgtcaggtg ctgtgaacac ttcttccgtc ggcatatttg
     1561 ccatgaattt ttctccacga gcattatagc ttccagcgcc ttcccaaaga tggtttttag
     1621 gcaatccgat aatgatatct gtaccgggag cagtatagtg aagggcagaa aattgttctc
     1681 gattcaattc ttctgctttt tttgctaatt tttcatcatg cttcttccat gctaagacag
     1741 ggtcttcttc atagacacga gtcgttttga aaatctgatc ccataatgcc tcaacttgtt
     1801 tttcttctgg cagttctggg aagacttttg ctgcccattg tttaccagcc gcagccacga
     1861 cagtccaact gactttattt gcttgagttg ccttgcgtaa attcattagt gctttgccat
     1921 tcgctgattg ataagacgct actcgatggc tatctacacc agcaaatgca tcgggatcgg
     1981 cagaaacaac gctgatcctg cttgctcctt tttcaagcca ttcatccgct tgatcgattt
     2041 tagattgtgg tacattctca atacgatccg ttgcagcatg taaaagaaat tctctttgga
     2101 tctggtcgtc agtccattga acgataacct cagcggctcc tagtttataa gcttcttgtg
     2161 tgatcaatcg agcaaggggt gcctgttcca cgctgatctg taaaacaact gtgtgtcctt
     2221 tttctgtggc tacgccagtt tctgcaatta agcgtgcata tttttttaga agttcattaa
     2281 aatcagataa cataataaaa attcctcctc accatttttt ctggtaacta ttagcatgat
     2341 accatttaat tatgattcaa acaatgaggc tttaaataaa aaaaggaggt tattttttta
     2401 aggttaagtt tcatgatata tgttactact tgctatttca gcttgtgaag aaaatcaaaa
     2461 actaattttg catatcaaaa aaacatgtta caatgaaatt aatcaatggt ggaagcgtta
     2521 taaaaaaatt aaggagatta aaaaatatgg caagaaaaaa gacgattact agagaacaga
     2581 ttttggcagc tgcttatgaa gtagctgcca ccgatggatt tacacgattt actgcgagaa
     2641 acatcgcgaa caaaatgaat tgttccacgc aaccgattta tcttgagttt aaaaatatgg
     2701 atgacttgag acaagctctt tttgagcaga tctatgatca tttacaatat gaagtgttca
     2761 ctgttgagca tacagggaat acgattatcg atcttgcttt taattatatc cattttgcaa
     2821 gaagagagaa gaagttgtat cgttcactgt acttagaaga atatggtgga ggaaaagaga
     2881 tgcagaattt ctcttatact tactttgtga atacagtgaa acgcgatcca gcttatgtga
     2941 atctttcaga agagcagatc gaatctttgt ataatgggac atggatcata gctactggga
     3001 tcgcagcttt gatgtcttca agcatcatcc atccaactga tgaacagatt gaaaagatga
     3061 tccaagacag tatagatgct atcttgaaat tcaaagaacc aatcgaaatc gattgaatat
     3121 actcaaaagt tccacaaatc agtaggtaac aagagatgtg tcaaaataag gaaaagttcg
     3181 tttttcttac agcgtatgca tttttaactc ccgaagaact cttccatgaa tgcaatgaat
     3241 aaaagagcca tacagattga ataaatctgg atggctcttt tattgcaagt atattaaatt
     3301 tttgcgacta gttctttagc gaaagcttct aaattttcga tatcttcttc ttcagcattc
     3361 agatccactt taacattttc ggctcctttt gatgcgcctg tttttgcaaa agcagcatcg
     3421 aaatcatcca ccgatttgca gaattcatcg taaaaagtat caccagatcc gcagacaccg
     3481 taaatttttc ccaatagatc caattcttgc aagtcttcat aaaagtctac gatctcatca
     3541 ggcagatcgc catcatcgta tgtataagta gctactacgc aaatatctgc ctcctcgaat
     3601 tcatctgcat ctacttgggt acattcgttg atttcgactt cgatatccag attttcaaat
     3661 gcttctgcaa caatatcggc gatttcttca gtgtttcctg tcatgctagc atatacgatt
     3721 tttgctaaag ccatgatgtg cctcctcgtt tataattatt taacaattat gaattatacc
     3781 aaaacatcat tgaaaagaaa ataaaaccta aagacacctg gggaatgtca tttggctaaa
     3841 tgtatgccgc tatgttaaaa tgagagggat ttgtaaaaag gagacgaaaa tatgattact
     3901 ttaaaatcag cacgtgaaat tgaaatgatg gcagaatctg gagctttatt agcagatgta
     3961 cataaaaatc tgcgtgattt tataaaacca ggaatcacaa gttgggatat tgaggtattt
     4021 gtcagaaact atatcgagag tcatggcgga attgctgcgc aaattggctt tgaagggtat
     4081 gaatatgcga cttgtatcag tataaatgat gaaatttgtc atggattccc tcgtaaaaag
     4141 cctttgaaaa acggtgattt ggtaaaagtc gatatgtgta tcgatttgaa aggcggcatt
     4201 tctgattctt gttgggccta tgtagttgga gaatctacac ctgagatcga ccgtttgatg
     4261 gaagtaacga agaaagcttt gtacatcggt attgagcaag cacaagtagg gaatcggatt
     4321 ggtgacatcg gtcatgctat ccaaacttat gtagaaggcg aaaatctagc gatcgttcgt
     4381 gattttattg gtcatggcgt tggtcctacg attcatgaag aaccagcagt tcctcattac
     4441 ggagaagctg gaaaagggtt gcggttgaaa gaaggcatgg ttatcacgat cgaacctatg
     4501 gttaataccg gaacttggaa aatgaaaatg gatcctaacg gctggactgc gtatacaaga
     4561 gacggcggac tttcttgcca atatgaacat acattggcta ttactaaaga tggtccaaga
     4621 attttgacat cacaaggaga agaaggcact tattgatcta tccagtcaat aggtacggga
     4681 gaggtacatg aaagtttgga acaaaataaa acaaaatgaa aatctgatgc gctttattga
     4741 gacgactcaa aagcgcatca ctgattcaga aatgagtacg acctctgtcg tcgttgctta
     4801 ttatttactc ttgtctttat ttccattgat tattgctttt ggtaatattt taccgtttct
     4861 ccacatcgat caagagactg tactgaccta tttacgagaa gtgattcctg agaccgttta
     4921 ccagtttatc ggtcctgcaa tcaaagacct tttgacacaa agttcaggag gcttgttatc
     4981 cgtttctgca ttagcggctt tatggtctgc aagtcaaagt atcaatgctt tgcaaattgc
     5041 gatgaataaa gcgtatggcg tagaaaatag aaaaaatttt ttgatcgtcc gtttcttctc
     5101 actaattgtc attgtcttgt tcatgattgc tattagtggg gtaactatcg ttcttgggtt
     5161 gggacagctg attttggatg ctattcagcc aatcgtacaa atacccgtga actttatcga
     5221 tcagttccaa actttgaagt ggccaatcac attggtagct ttatttgtca tcatgttctt
     5281 gatttattta attgtcccta atgctcagtt gaagttgaag acgatcattc cgggcacgat
     5341 cttcgctact gtcggctgga tgctgttatc acaagtgttt agtatctatg ctaggtattt
     5401 tgcaacgagg atcagcggtt atcagatcat aggaagtttt attgtactca tgctttggct
     5461 taattttgct gctacgatca tcattttggg cggaatcatc aatgcagttg ttcaagaata
     5521 cgtgacgggt catcaaatca aaaaacgtcg agatccaagc cgaaaatggt tcaataaatt
     5581 gaaaactaaa ttttctcata aaaaaaagta gaaacaagag ttcttattgt aaatttatat
     5641 cagtaattta taataattat gactcttaat taaagtaggt gcccatatgc ttgtttcttt
     5701 tagtatggtt ataagatttt ttctgacaat tttattatca ctgatattaa cacctgtatt
     5761 caaaatcatt tcagtacaaa caggtatggt cgacaaacca aatgaaagaa gaataaataa
     5821 agttccaatg ccttcagcgg gcggactgcc tatctttatt tcatttgtta tatcgacttt
     5881 attcttattc cgtaaaatta tccctcaatc gtatattgta ccgatcttga ttgcttcttt
     5941 agtaatcata gtcacaggag tgctcgatga taaatatgag ctgacaccta aacaaaaaag
     6001 tatagggatc ttgattgctt ctcttattat ttattttgta gcagatatac gtattgattc
     6061 cttcacgctt ccttttattg gatatatcca actaggctgg cttagttttc ccgtaacgat
     6121 tttttggatc ttcggtatca cgaatgcggt caatctgatt gacggtttgg atgggctggc
     6181 ggcagggatt tcattgatcg gtttaattac gataggaatc attggatatt ttttcctcca
     6241 tgcttctaca gtgtatattc cagttgttat tttctgtctt gtagcaagta ttatcggttt
     6301 ttttccttac aatttttatc cggcaaagat ttatttaggc gatacaggag ctctattcct
     6361 agggtttatg atggccgtcc tttcactaca agggctgaag aacgtgactt ttgtttcatc
     6421 gatttctttg ctgatcgtca tgggagtgcc ggtaacagac acgttttttg ctatcattag
     6481 gagaaaagca aaccgtgttt ctttttctac agcagataaa aaacatcttc atcaccgact
     6541 aatcagttta ggctttactc ataaaggtgc tgtcctgacg atttatgcga tggctttgat
     6601 gttttctttc acagctatgg tcatgaatta tactggaaga atcggaacga tcatattgat
     6661 tattgccatg ttgtttgctg caattttatt atttgaattg attggtctga tcaatgagaa
     6721 acatcagttt attttaaaaa cattgcgatt tttagggaat aaagagtacc gtacgcaagt
     6781 aaaacagaaa tatttgaaga aatggaaccg tcatatagat gaataacaaa ttaaaccagc
     6841 cgatccttat tagtattgtg acggcaaaca gtgataaaat ttttcggaca ctagataatt
     6901 ttatttcaac tgtaaaagat gaaacagcag taaaaatccg aatttttgac aacaattcac
     6961 ttcccgagta caaaaaaaga ctaacagagt ataggaaata tccctacatc gatatctatt
     7021 ttcatgatga aaattccgga tttggttatg gacataatca caatctgata gctagcgatt
     7081 ctcgctatgg cattatttgt aacccagata ttttagtgga tgaagagaca gttacagcat
     7141 tggtcttgtt attgaaagaa catccggaat gtgcgatgtt agcacctaag attcttaatg
     7201 aagatggaac aactcagcac ctcatccgtg agcagctggc tgtttttgat tacgttttgc
     7261 gctttgtgcc tttcaaatgg gtcaaaaaaa agtttgacaa acgactggct gtgtttgaat
     7321 gccgaggatt gcctactgat gctaatagtt atgtacggat gatctcaggt agttttatgg
     7381 tagctgatat tgataaattt aaagagataa aggggtttga tgagcgtttc ttcatgtact
     7441 ttgaagacaa cgacttgtgt ttgagttttg aaaaagcagg ttacaagtta ttgtacacac
     7501 ccttttatgc agttatccat ttttacggga agggtgcgca tcgaaatgca aaactgtttc
     7561 gcgtgttttt gacttctatg atgaaatttt tcaataagtg ggggtggcgt tttttctgat
     7621 gaaaccagaa atattagtag tcgttgtgct ttatcagcaa ctattcagtc aatctccagc
     7681 ttatggacca ttaagcaaag cacttagcga aaagcaaatt cagcttatta tttacgataa
     7741 tagtcctcaa aggcagcaaa atgagctgtt tgaaaaagaa aatatcgttt atcatcatga
     7801 tccgcaaaat cctggtttag cggtggctta taattttgcg ttgtctcaag cagatgaagg
     7861 aacacgttgt ctcgtcactc tggatcagga tacgaggcta gtcgacgatt attttgaact
     7921 attgcgtaaa gtaaccttta cagatgaatg tgttgctgca gttccgatga ttttttctgg
     7981 gagccgacaa gtttctcccc tatattcaga tcgttatatc aatcgtacag ctcaagcagt
     8041 agaagttgaa ggcccaacag ctgaacgaat catggcaatc aattcaggtg ttgcctggtc
     8101 tgtcaaattt ctaaaaaaga ttggcgggtt taatcctgct ttttctttag attttttgga
     8161 tcattggctg ttttggaaag tggatcagtt ggaaaaaact gtggagatcc ttcctgtacg
     8221 aatggaacat gatctttcgg ttttagacta tgaaaaagtt tcaagtaatc gataccgttc
     8281 tattttacaa gcagaaaatc gattttatca tgaatatgat caaaggcatt tatccagtca
     8341 ccgacgacaa ttattgttaa gaacagctaa acagttcttg accgtcaaaa accggcatat
     8401 ctggcggatg actttgcgct catatgttaa gaattggaag gtgtgaattc gtgcgttcgg
     8461 tttgtatagc tacctttaat ggaggcagat atttaagaga gcagttagat agtattttat
     8521 ctcagctttc agcagaagat gaagtagtca tttctgatga tgggtcagta gatgatacat
     8581 gggatatatt aactgaatac gcagaaaaag ataagcgaat ccagcttttt tcggggcctc
     8641 gtcaaggatt gatcgctaat tttggttatg cgatccagca gacgaaagga gatctaatct
     8701 ttttggcaga ccaagatgat gtttggttgg agacaaaagt gaaaaagatc actgactatt
     8761 ttgagcagaa tccggataag ctagtagtag tttctgattt aatcattgtg gacagcgagc
     8821 tgcaagagat ccatgattcg tatttttcgt ttcgaaattc caaagaagga tggcttaata
     8881 atgtgataag aagtcactat attggtgctg gtatggcttt ccggtcttct ttaaaacccg
     8941 atattttgcc gatcccgtca gaagtgccga tgcatgatat gtggattggt ttgctgggag
     9001 gaaagaagac tgggttccta agagaaccgt taactctgta ccgaagacat gatttaaacg
     9061 cgagtgagat tgaaacacat tctaaattac ggcaaaaaat ttcttggcgt gttcatctga
     9121 ttacggcgtt gatcaaacga aaatggttta aacgttgata aaagatgttt aaatccttaa
     9181 tatttccatg acaaatataa caattgatga gataataaca aagtgaaatt tgaaatcaaa
     9241 aaggagagca gatatccatg aagggaatca ttcttgctgg gggcagcgga acacgtctgt
     9301 accctttaac aaaggcaaca tcgaaacaat tgatgccgat ttatgacaaa ccaatgattt
     9361 attatccaat gtctactctg atgttggcgg gaatcaaaga aattctgatt atttcaacgc
     9421 cacaagatac accacgtttc aaggaattat ttggaaacgg aaatgattta ggactgcaga
     9481 tcgaatatgc agtacaagaa agtccagatg gattggcaca agcgtttatc attggagaag
     9541 aatttattgg agatgatagt gtctgcttgg tattagggga caatatttac tatggtggcg
     9601 gtctgtctaa aatgttgcaa cgagcagctt caaaagatac aggcgcgaca gtatttggct
     9661 atcatgtcaa tgatccagaa cgctttggtg tagtagaatt tgatgagaat atgcaagctt
     9721 tgtcgattga agaaaagcct gctcagccaa aatcaaatta cgctgtaact ggcctttatt
     9781 tttatgacaa tgaggtaatt tctatcgcta aaggtatcaa accttcagaa cgtggagaac
     9841 tagagattac ggatgtcaat aaagcatatt tggataaagg aaaactatcc gtagaactta
     9901 tgggacgcgg atttgcttgg cttgatacag gaacacatga atcattgctt gaagcatcta
     9961 cctttatcga aacaattgaa aaacgtcaga acttaaaagt ggcttgtttg gaagaaattg
    10021 cttaccgtat gggctacatc gataaggaac aattgctggc ccttgcacag ccattgaaaa
    10081 aaaatcaata cggtcaatat ttattgaatt tggccgctga ataaggagtc cttacagatg
    10141 aaagtaacag atacaaaatt aacagatgtc aaaatcgtag aaatggacgt ctttggcgat
    10201 catcgcggtt tcttcacaga aagttattca aaagcaaaat ttgcagaaca tggattgaaa
    10261 tatgactttg ttcaagacaa ccattcacta tcagctgaag ctggtgtcct tcgggggctt
    10321 catttccaaa aaggagaagc tgcacagaca aaattgattc gggtagtcac tggagcagtt
    10381 ttagatgtag tagtggatat ccgcaaaggt agcccgactt atggtcaatg ggacggattt
    10441 atcttatctg aacataatca tcgacagttg cttgtaccta aaggatatgc ccatggattc
    10501 gttactttga caccaaatgt gaacttcctt tataagtgtg ataattacta taatgcagaa
    10561 gcagatgggg gcatcgcatt tgatgatcca gatttagcga ttgattggcc aattgatcca
    10621 tcaagagcga ttatgtcgga aaaagatcac cgtcatccga cattaaaaga atttgaagca
    10681 gaaaatccat ttgtttacgg agaaatctaa gaattaagga gaaattttct tatgaaaaac
    10741 atcattgtta ccggaggtgc cggattcatc ggctctaatt tcgttcatta cgtggtaaac
    10801 aatcatccag aagtgcatgt cactgttttg gataaactga cttatgcagg aaacaaagag
    10861 aacttggccg gacttcctag cgatcgcgta gaattggtcg taggagatgt tgcagatgca
    10921 gaattagtgg accgtctagt aaaagaagcg gacgcagttg ttcattacgc ggcagaatcc
    10981 cataacgata attcattaaa agatcctttc ccatttgtac aaacgaatat cattggtacg
    11041 tatacacttt tagaagcttg ccgcaagtat gatgtacgct atcatcacgt atctactgac
    11101 gaggtttacg gcgatcttcc attacgtgaa gacctgccag gacatgggga aggcgaagga
    11161 gaaaaattca ctgcggaaac accatataat ccttcaagtc catattcttc tacaaaagct
    11221 ggttcagatc tgttagtaaa agcatgggtg cgttcttttg gcttacgtgc aaccatttca
    11281 aactgttcaa acaactatgg tccttatcag catattgaaa aatttattcc tcgccaagta
    11341 acgagtatct taagcggtat tcgtccaaaa ttatatggcg aaggaaagaa tgttcgtgac
    11401 tggatccata cgaatgatca ttcttcagca gtgtggctga ttttgacgaa aggcagaatt
    11461 ggcgaaacct atctgatcgg tgcagacggt gaagaagata ataagaccgt gatggaattg
    11521 atcttagaaa tgatgggcca accaaaagac gcatacgacc atgtaaacga ccgcgcaggg
    11581 cacgatttgc gttatgcaat cgattcgaca aaattgcgag aagagctagg ttggcagcct
    11641 gagtttacga acttccgtga tgggttggct gacacgatca aatggtacga agaacatgaa
    11701 gactggtgga aaaaagaaaa agaggccgtt gaagcagcat atgccaaaaa tggacaataa
    11761 tacagaggaa atttcactgt attaatggaa aaacacatgt gaggcaggcg ctcgcctgtc
    11821 tcttttttaa aataaaaatc aaggagcgga taaccatatg tttttattaa ctggaggaaa
    11881 tggacaatta ggtacagaat tgcgccattt attagatgaa aaaaatctgc catatgtttc
    11941 aacagatgca aaagacttgg atatcacaga tgctgagaaa acaatggctt atattacaga
    12001 attaaaaccc gatgttattt tccattgcgc agcttacaca gcagtagata aagcagaaga
    12061 agaagcaaaa gaattagatg agaaaatcaa tgtcgatggc acaagaaatg tagcacttgc
    12121 agcaaaagct gtgaatgcac aattcgtcta tatcagtaca gactatgttt tcgatggaaa
    12181 aaacaaaaat gtagaatacg aagtagacga ccaaacgaat ccactaaatg aatatggtcg
    12241 tacgaaatta ttaggcgaaa aagcagtaga agagatttta gaagactatt atatcatacg
    12301 aacttcttgg gttttcggga tctatggaca caatttcgtc ttcacgatgc aacgcctagc
    12361 ggaaacccgc gacaaactga ctgtagtaaa tgaccagttt gggcgaccaa cttggacacg
    12421 tactttggca gaatttatgg tatatgtcat cgaagaaaaa gcaccatttg gtatttatca
    12481 tttgtcgaac gaaaatagct gttcttggta tgagttcgca aaagaaatat tgaaagatac
    12541 agatgtagaa gttgcacctg ttacgtcaga agaatttccg caaaaagcaa cacgtccgca
    12601 gtattcagtc atgagcctga aaaagacaga agcgttaggt tttgtcatac caacttggca
    12661 agaagcactt gcgcaaatgc tggaagctgc caaaaaaatg aataagtaat ttccttttta
    12721 tcaaatagaa tttggagcat gatatggaaa aacaaacaag aagtgcacga cgaaaagaaa
    12781 agccaaaagc gaatcaggca gattcgaatt tggtgcttga cctgtttcta aaactccgct
    12841 atcttattgg cgttttagtc atcgtggtca tcgttacttt taatttaaac ggaagttcac
    12901 ttggtgtttg ggataaatat gtgtcgcaaa gagacgatgg gaaaaaaact gatgtgattt
    12961 ttggacagaa tcgggaagtg cggtcagacg aatggctggt gcagacacct ttttacctct
    13021 cacaagctga agaggacttt tcgttggtga acgaggatta ttcattaaac ggacaaaaca
    13081 tgatcattgc gtataattct cctgtaaagg atattacggt tatcggaaaa ccattcaact
    13141 ggggattctt tttcctcgga agagatcgag gactttcctt ttattgggcg atgaaattag
    13201 tcgtcatggt cttgcttgga ttcgaagtac tgatgatatt aacgaagagg aaaaaagctc
    13261 tatcgctaat cggtgcattt gcgctgacat tttctccagc agtccagtgg tggttcatgc
    13321 aacatgtggg cgatttgatt ttctttactt tgggactgat gattgctttt tatcattatt
    13381 tctacaatca tgataaaaaa tggctacgaa cgttgatgat gcttttggtg gttattttcg
    13441 gtattgggtt tattttagtg atttacccag cccatcaagt gatgctggcg tacttgctga
    13501 ttttttattt tgttggcctg cttatttact atagcaagaa agtttcatgg gatctgttgg
    13561 atgtagtgtt gattggaggg gcagttttat ttattggcgg agtcatggtt catttctggc
    13621 taacgtcaaa agacgcattg ttagcttctt tgaatacctt atatccagga aaacgggtaa
    13681 gtactggagg aaagtggacg attggcgaat ttttttcttt ccttacaaat tggaaaattc
    13741 cttttgaaga tatcaactat tcgaataact ctgaagtggc cctgttctat cacttttttc
    13801 caactgtatt cctagcaagt ccatttgtgt tatttggcaa aaaagatagc gaacaaaagt
    13861 tactgggacg tattttgatg attttttgtc tgttttctat cttttggatt accgttgggt
    13921 tgccaaaatg gctagcagaa atcactctgc tgtcttatgt tcctccatta agagccatcc
    13981 ttacttttag ttttgcggct tgtttattat cgatctggtt tatcgcttat atttggagag
    14041 aagatgttat tcctatttgg tataaattca ttttgttggc agtaaatgca tggtcttatt
    14101 tctatgcgat tacgcattcg aaaatggata attatttttc ggattttgaa accatcgcag
    14161 ttgctgtgtt gggaacagtg attttagctt ttgcattatt tggctggaga cgcttgtttt
    14221 acctcgcttt tgtcgcctta gttatcgtat ctggcttttt cgtcaatcca gtcgtagaag
    14281 gtacaggagc gatttttgaa aaaacactgg caaaagaaat ccaaaaaatc gatcaacaag
    14341 atcctggtaa agtttggttg tctgaagatg aattatataa cttcacccca acattgggag
    14401 tgaaatcctt caatacagta cgcttttatc cagatatagc tgcatggaag aaaatcgatc
    14461 cgaaagaaga atacgagaag tattataatc ggtatgctca tacaagagct tttatcgcta
    14521 aggaagatac aaagttcatt ttagatcgtc cagatatgtt ttcggtcacg ttgaattttg
    14581 aagacgcaga aaaattaggg atcgactatg tgatcagtaa acgaccatta actgattaca
    14641 accaattata tgaagcgcag ttcacgcagc tatatggacc ggataaagac ggttatatga
    14701 tttttaaagt agaatatccg gattatccag atgtaccaca aacacaaact caggaaaatc
    14761 agctagtcgt acagtaatag gctagctgag aataatcctg aaatattagt agaaaggaga
    14821 gtgaagataa ttcaagcaat cgtacaaaaa tttgtcaaca aaatgagtgt cggtttcttg
    14881 cttttgttgc tgacgctagt cttttttgtt tggacagcag gggtaggttt tccagcaact
    14941 ttgtttatat ataacaatag ttcattactt atcgctttta gtgtggctat tttgctcatt
    15001 ttaaatgttt ataaagcaaa aggcgtcgat tttctttatc taggtgttgc gcttgccctt
    15061 ttcattttct tttcttactt tcgggaatta agaaatgagt ctttatttgg agacttactg
    15121 tttcctttga ttattttatt ttgtttagta attaaatgga tcgagtggac aaaatgggat
    15181 cgcggtattt atctgattgt ctttacggtc ttcctcggaa tcacgttata tcgtgtattt
    15241 acagagatcc aagtaccaga aggacaaagc atctgggctc caggcaattc gctttctgat
    15301 atttggatca atacgaatac aatcggaagt tctttgatga ctcttgcttt attgatcagt
    15361 ggatttgcca gttcttttga acgatggtat gttcgaatca tcggtgggat agcagtcgtt
    15421 gcagcctttt taggtatttg ggtctgccaa tctagaggtg cattagtcgc gttgattgtt
    15481 tttgcgatat tggatctttt acctaaatcg ttgatgcgtg tcattcgtgc gccatttatt
    15541 gcctacacgg ttacgattct tttagctttg ccgatttctt atctggcggc tgtctctgaa
    15601 aaagtcaatt tgtttaccgg tcgagaagat atttggcata aattctatca gacgctagga
    15661 gaaaaatctg aacaagtttt actaggaatg aaaacattta tcttccagcg aggaaaccaa
    15721 tttttaggaa accacaatag ttataattct attttgaata tctatggatt gataggattt
    15781 gggattgctg ctttgctgct tatccttttt attggtcgct taacgttgaa agcagattta
    15841 tccaacgggc agatgacttt tatctgggcc ttttttgcgg tgatgatgca gtcatttatg
    15901 gaggatacat tgacttcata ccgatgggtt ccgattattt atatcctgtt ggctatggcc
    15961 gttcatcgat atgatgaacc agaagttacg gaggaattct atgttatgga agaggcatat
    16021 gacgatatga cttatgatga tacatcttat gaggaacaac cagatagcgc tcattcttcc
    16081 aggatccaac gctatcattg ataggcacaa aatacgagag aggctgtgac aaccgtcgca
    16141 gccttttttg gtttattgca attgtctagc atcttctgta aaatagaaac gttagagaaa
    16201 atcttgtatc atagaaaatt aagaaagaaa taagaacgca gctcggtacc aaaaatatgg
    16261 taaaatggca cttgactgta attcactgaa aatcgcttgg aggaacacaa atgtttaaag
    16321 atttgtattt atttatgaag gacatttatc agaacagaaa attactgcta cagttttcga
    16381 tcaatgactt caaatcaaga tatgcaggat cgtttttagg aatcttatgg gcgtttatca
    16441 atccgctatt tactgtattg atttattggc tggtatttgg tgttggactg aaagcacaaa
    16501 tgatcgatgc aaaataccca tttatcgtat atttagttac cgggatgatt ccctggttct
    16561 tcttctcaga tgcatttgca tcaactacgg ttgtttttcg ggaatatact tatttagtaa
    16621 agaaagtcgt attcaatatt cgtatcttgc ccacgacgaa aatcctatcc aatctgtaca
    16681 cccatttatt cttcattctg attggcatag ctgtcgcgct tataaatggt atatatcctt
    16741 cagcaatgtc tttacagctg atttattatt tattttgcat gtgtgctttc ttgacaggat
    16801 tgacttggct gacggcatcg atccaaccat ttttacctga tatcatgcaa ttaattacga
    16861 tcgtgctgca gttgatcatg tggacattac cgatattatg gagccctaat cagtttgatt
    16921 caggtcttgt aagcatattg aaattaaatc cgctgtacta tgttgttcaa ggttatcgtg
    16981 agtcattttt aagtgaagct tggttctggg agcatggaac atatactttg tatttctggg
    17041 ttttcacgtt agtaatgttc gtaatcggct ctgcagtgtt ccgtagattg aaaccacatt
    17101 tttcagatgt gctgtaagga gaatagctat gacagaatat gcaattagac taaaagatat
    17161 cacaaaatct tataatatgt acgcaaaacc ttcggatcga tttcgtgaag ccctcaatcc
    17221 ttttaagaaa tcctatcatg atgtatttta tgcgttgaaa aacgtgaatg tagaaataaa
    17281 aaaaggtgag atgatcggtt tcgtgggaga aaacggatcg ggaaaatcta caatgttgaa
    17341 aatcattacg ggagtactga caccaacatc cggcacgatg gaaatcgaag gaaaaatctc
    17401 agctttgcta gaactaggat caggattcaa tcctgagtat tctggctatg aaaatatcta
    17461 tttaaatgga atggttctag gattttcaag agaagaagtc gatgaaatgg tcgatgatat
    17521 cattgaattt gctgacatcg gcgaccatat ttatcaacct gtaaaaacct attcaagtgg
    17581 gatgttcgtt cgtctagctt ttgctgtagc gatcaatgtg aatcctgata ttttgattgt
    17641 ggatgaagca ttggcagtag gagatcttga atttcaacta aaatgtatgg aaaaatttac
    17701 agaaatcaag aactcgggaa agacgatttt attcgtttct catgatatta gttcgattcg
    17761 ccgtttttgt gatcgttcct attggttgaa aaatggaaaa gttgtcgaat atggtgatac
    17821 gatggaagta acgacgaatt acgaaaactt tttgaagaaa aaatcagtta aaacagtaga
    17881 tcgaaataaa aaccaagtag aacgcttcgc agctccagat atcgtagacg tgacgaaagc
    17941 cgaattgttg gataaagata aaaatccagt ggatatgctg gaacaaggag aaaaactata
    18001 cgttaaagta acctatgaag taaaagacga tacaatcaaa aatccagttt taggaattgc
    18061 tctgcgtaca gtcgacaatt tttatgtgtg cggactcaat actctactag atggtatcaa
    18121 gatcccttgg aaaaaaggtg agaatatctt ttatctggag tatgaaaagt taggtttgtt
    18181 agctggtgaa tattattttg acgttgcggt ctttgaagaa aatgcaacgg ttcctttggt
    18241 atacaagaca aaatacatga acttgtttgt cagcggttct tatatcggag aaggcatcgt
    18301 ggtcttagac cataaatggg aagaagggac tcatagcaat gaaatatgat tttgaaatgg
    18361 acctagatga acaaagcagt gtgggaaaaa ttgccgcaca gatcaaacca ggaagtaaag
    18421 ttttagagtt tggtcctgga aacggccgat taacgaaaca tttgatcgga gcaaaacaat
    18481 gtgaagtgag cattgtcgag ctagataagg aattgttcga ttttgtcagc gagtttgccc
    18541 aagatggatt ttatggagat atcgaaagtt ttgaatgggc aaactattat gctggacaaa
    18601 catttgatta cgttttgttt gccgatgttt tagaacattt ggtagatcct ggaaaaacgt
    18661 tgaagaaagt tagagaattt ttgaatgaag aaggagaaat tctaattaca ttccctaacc
    18721 ttgcgcataa ttctgtcatg attgatttgt tcaataatca attgccatgg gcttcttatg
    18781 gtttgctaga tgaaacccat aattcttttt atactcatga tggcttccaa aaagtttttg
    18841 aaaaagcagg attatacatc aatattgaag attatttgta cttagctgta ggtgatacag
    18901 aattaaaatc aacttatgaa gaattgccgg aagctgtacg ttatgatttc aaaatgcgac
    18961 catttggcga agtctaccaa tacttcttct cattgatgaa acatccagta gcacaaagtt
    19021 ccattgcgca gccgcaaaat tctaattatg tgaaagtatt agaagtaatc cagcaaacga
    19081 aacaagatga aataattcaa caaataccat tcaataattt cacaggggaa aatgagacac
    19141 taagtttcca tgtatcagaa ttagcagaac gaatggtgtt tcgcttcgcg cagcaaccta
    19201 gctttatcga attcagcgca gaagctgctg gagaaaaact cacatttatc gattcaaatg
    19261 ctgtagtcaa aacagtaaat gattgttatt tgtttgatgg taaagattta ccagagttct
    19321 ttttaacgga tattgcaggg aaagaagtga ctatccattg tcactatcgt tttatcggag
    19381 aattgacacc aacaatgaaa gaattgctgg aaacaatccg tccaatggct gaagttacaa
    19441 agcagctttc tgagaaaaat gatgaattag aaagagaaaa tcattatctt attgagaata
    19501 atacaagatt agatcagacg ttgaaaacaa ccataaatcg ctattgcacg ttgttggaaa
    19561 gtgatgaatg gacaatcaaa catagattag ggcgacgtaa gaaagaaaca tcgaaaaaaa
    19621 tccaagaaaa agagctttct ctatgtattg acgaaaaaat ctgggatgca gaaacaaaga
    19681 tattgaaaat catcgggtgg ggaatcgcta attcggatcg ccaaccattg tcctataagt
    19741 tatcagccga ccaatcacca ttttttgaag caattcctgt tttacgagaa gaagtcaatc
    19801 aagcaaaaca gctagctgaa ggagcaaaag caggatttga actgcggatc ctttgcgaga
    19861 aggaacgttc attcttagtt gaagcagtgg ctcaaaatgg tcaatcatgg atcattgaaa
    19921 tgtaaataat gattaactta taaagataag gagtaaacga atcgtgcaaa aaaaagaaat
    19981 tgccgtattt atcgatcaaa tcactcgtga acgggccact ggcgatttgc ttattgtggg
    20041 ctgggcaatt gatgaagtga ccaaagaaat ccctacgata aaggtagaaa aagaaaatgt
    20101 gattgctgaa gcaacacatg tcgtaagatt ggatatcaat catttatata atcttgatgt
    20161 taagacccaa agcggtttta aggtacgttt atcagggaaa atgagaggaa aagcaatctt
    20221 agacttccaa acagcaagac atcaaaatgg catagctgtg aaattaaacg gtaaatatcc
    20281 ttatgatgac ggcattgaat cttcatggga aagaaaaaaa agattactga aaaaagggat
    20341 aaattatgca aggactcatg gcgtaaaaaa agcaatccgc cgggtcaaac tggaattgaa
    20401 accaggatcg atcgactatg cagagtggat cagtcgtcat gagaaacctg atctgaaaga
    20461 gcaaaggaaa gaagtacaag gatttgccta ccgcccattg atttccatac ttatgcctgt
    20521 ctataacgtt gaaatcaaat ggctcgaaaa atgcattgat tcggtacttg atcaaacgta
    20581 tgatcactgg gaactatgta tcagtgatga tgcttctact gatccagcta tccgaaagtg
    20641 tctagaatcc tatcaagcaa aagatgatag aattaaagtc gttttccgac aagaaaatgg
    20701 acacatcagt ctggcaacta attctgcatt ggaaatggca gaaggagagt ttatagcgct
    20761 cttagataat gatgacgaat tacctccatt tgccttatat gaagtggcaa aagtgctaaa
    20821 cgtacatcca gaactggatt tgatctacag tgacgaagat aaaatcgatg cagatgggaa
    20881 tcgctttgac ccgcacttca aagcagactg gtctcccgat acattgatgg gaaataatta
    20941 tatctctcac ttaggcgtat atcggacaag cattgttaag gaacttggcg gtttccgtaa
    21001 aggatatgaa ggctctcaag attatgattt ggttcttcgt gtgacagaac aaattcctgc
    21061 agaacacatt taccacattg accgtgtctt gtatcattgg cggacgatcc cgggatcaac
    21121 tgcaagcaat ggagaagcta aaagctacat ctatgattct ggagtgaaag cattgacaga
    21181 tgctctatct agaagaaata tcaagggaag cgtacatcct ggcagaattt ctggttttta
    21241 tgaaatcact tatgatgttt tgcaagaaga cttggtcagt gtgatcattc caactaagaa
    21301 tggctatgaa gatctaaaaa cttgtgtgga ttcgatcatc gaaaaaacgt cttatccaaa
    21361 ttatgaaatc attattgcag ataatggaag tacagatcct aaaatgcaag aattatttgc
    21421 agaatacaag catcagctga aagaccgatt tatcgtcgaa ttaattgaca ttccattcaa
    21481 ttattcacgt atcaacaatc tggcagcaga aaaagcaaac ggcaaatact ttttattctt
    21541 gaataacgat acagaagtga ttgaacctga ctggatgaca gcgatggttt cttatgcaca
    21601 gtttgatcga atcggctgtg taggagcaaa attattttat ccggatgata caacgcagca
    21661 tgcaggtgta ttattaggga tcggtggcgt tgcaggacat gcattgaata actatgatcg
    21721 aacacattgt ggttactttg gccgattggt cattgacgtc aattacttag cagtcactgc
    21781 agcttgtatg atggtcaagg cagctgactt caatgctgtc aatggatttg atgaaatatt
    21841 agaagtagca ttcaacgatg ttgacttgtg cttgaaagtt tatgaactgg gtcgttacaa
    21901 tgtctatgca catcaagcag aattgtacca tttcgaatct aaatcaagag gttatgaaga
    21961 tacaccagaa aaacaaagac gttttgctgg ggaaatcaaa aaaatgcaag ataagtggcc
    22021 ttcttatatt gctcatgacc cattttataa cgataatcta actaaagaag gaatcggcga
    22081 tttttcgttg agacctgatt gattaaaaga actgtatttt catttttatt aatgaaaata
    22141 cagttctttt aatttaatta tatgatttgt caaattaagc tagaataaaa tgcttcttgt
    22201 acatagctca aaaatatttc gattggtgtt ctgtaattca atgattttct tggaatatga
    22261 ttacgttgat tgctgacact ggaaataaat gtctgattca cttttctaaa atccattgat
    22321 ttcggcagtc cattacgacg cagaatcccg ttagaattct cgtttaatgg gcgttgagaa
    22381 ggtgttccag ggtccgcaaa atatatatca atatcatgtt ggttactaat ggctttccag
    22441 ttagaaaatt cttttccaca gtcaaacgta atagatttaa agaagttttt agggaagcga
    22501 gaaaaccatt gattaagggc agtttcaata tctaatgcct tacggccgtt gggtttaatc
    22561 gtgataatga ctttagataa tctttcaact aaagtaatga cggcactttt atgatgaatg
    22621 ccaacgatcg tatcaccttc aaggtgacca aactcagaat tgaaatcagg ataattatca
    22681 ggtcgatcat ggattgagcg ctgatactgt tgttttcccc gtttttcctg atggccattg
    22741 ggttttcttt tacctttcat cggtagtgtg tcaatatcaa atattccttt agaaaataaa
    22801 cgataaagtg ttctcatacc gcatgaaaca ggcctttctt ttcgcccgat aatgacgtca
    22861 ggcgtccaac caagagtgac tttctcttta atgtaatcta cttcatgagc aggtaattga
    22921 atgacttttc taccacattt tttcttattt tctttatact ggtgccaata atcaagagca
    22981 gtcttgcctt gcttgaactt attgactaca ttataaatag tttgtatagc acgtcccatt
    23041 cggttagcga tttcaaccgg cttattatgt tgaagataat atgattctat cattgtcagt
    23101 tcgtctatgg taagatgttt gtaggtcatt tatggttaca ctcctttgtt ttctttcgtc
    23161 ggaaatacaa tttgagtgta ccataaatgc ctttttattt ttctaactta attttacaat
    23221 tcgcgttata tgaatattgt aacttgtgaa taaggaatta gcaggagaat actagttcag
    23281 cataaaggaa gtgatgggac ttctgagaca actttctgtt atgcagaaat acacatataa
    23341 acagacaata aacgcttttc cccgtttctt ttaatttcct ttacaatttg gttattcact
    23401 aggtttttaa actttgctat ggtaagatga tagaggcatt attttatggt taggagtgta
    23461 tatatgaata aaaatgggga gtggaatgcg gcacgcagaa tattcattat tcttatggat
    23521 gtacttgtat taaatgtatc gatctatctt agttttttgc tgaaattcca gggagaagta
    23581 ccagtgagaa acttagaaac ctttcagcat tcagcaattt ttatttccat catctttatt
    23641 gcgttgaata ttttgttagg agcttatgta ttctataacc gaatgatcag tgatattgtc
    23701 ttcattactg ttattggaca agtactaata gcattgggga tcatggtagt tacttttgct
    23761 ggccgatggt ttgcatttcc gcgtggcgtc attttgatct cgtttatcct aggtacgata
    23821 ttacttagtg tttaccggat agtcgtttac aaactgtact taagagtcag tcgtgataag
    23881 aaagtcctta tcgtgggttt ggagaaagat gtcgcaccag ctatccaaaa ttttcaatct
    23941 aagaaaaaca ataaacataa ggttgaagca gtggcaatcg ataattatta cgaaaataca
    24001 aaaaaattga tcgatgatat ggatatcgtc tatttggctt ctaaaatccc agaagatgaa
    24061 aaaatcaaca tttatcaatt agtgactaaa aaagagaaga aattattttt aaatacgact
    24121 tttgagaatc tgacaatgat aaatccgaat attatgaatt ttgaagatga aagcatcatt
    24181 gaagcttctg ggttcagaat ccaaccagaa tacgaactat tcaagcgtct ctttgatatc
    24241 atcgtttcgc ttattttact gatcctcgcc tcacctttta tgctaatcac agcgattttg
    24301 gtaaaaacaa cttcacctgg accaatcatc tataaacaag tacgtattac gaaaaatcaa
    24361 aaggaatttt caatctataa attccgttcc atgagtgcga cagctgaagc aaaatcagga
    24421 ccagtcttgg ctaaaagtaa cgatgcacgt gtgacaccag taggtaaatt tatccgtgct
    24481 gtacgttttg acgaattacc tcaaatcttt aatgtattga aaggcgatat gtcgattgtc
    24541 ggtccaaggc cagaaagacc atttttcgtt gatcagttca atgaagaaaa tccttattat
    24601 tacttaagac acaatgtacg tgcgggaatt accggatatg cacaagtata cggaaaatat
    24661 gtatcagatt ataacagtaa actacgtttt gatttgttgt atatcaaaaa atattcatta
    24721 ctattagatt tgaagatttt acttcaaaca atcaaaatct tatttgataa gatgtcatct
    24781 caaggactag atgaagatga aaatattagt tcattagcag aaatcgactt ttcacaagag
    24841 aaaatttatc gctagattgg aaaaattttg agcaaagagg taggagagaa cattggaaaa
    24901 taaaaatgta agtatgctgc tttctttata tgataaagaa aagccagagt atttaaagga
    24961 agcattaact agtatttttg agcaaacagt aatgccaaaa gaaattgtat tagtttatga
    25021 cggccctata aatgaaaaac ttcaagtagt tgtcacttat ttccaacaaa aagatcctca
    25081 cttgtttaca gttgtaaaat tagaaaaaaa tcaaggattg gggattgctt tagcaattgg
    25141 attagaacat acgaaaaatc gcttagtagc cagaatggat acagatgata taatggaacc
    25201 ccaacgatta gaaaaacaat tagagatctt ccaaaaagat ccagaaatag cgattgttgg
    25261 gagtaatatt gaggaattta ctggcacgct tagcaatgtt atcggaaaaa gaattgtacc
    25321 tgaatataac aatgcaattc gtgtattttc taaaagaaga aatcccttta atcatatgac
    25381 cgttatgttt gataaaaaag ctattttgga agtgggaaat taccaaccat taagtgggtt
    25441 tgaagattat tatctttggg caagattatt aaaggcaggt tataaaggat ataatattca
    25501 agaaacatta gtctatgcgc gaactggcaa agatatgtat gcaagaagag gcggaatgaa
    25561 atatcttggt tctggtctta tgggtcgtta tcgtatctgg aaagacggtt tagggtcagt
    25621 aaaagatttt ataattgttt cttgcggaca tatttttatt agcttactac caaatcaatt
    25681 aagaggaaaa ttttatcaaa cgcaattaag aaaataggag gtttttcttt gtatagcact
    25741 gatccaaaaa tgaaaaaaat acaacagatt acgttgaaaa tgtctcaaga gattgttgat
    25801 ttttgtctaa aaaatcaact tctttgttat ttctgcggtg gtggtgccat cggtactgtg
    25861 agagaacaag ggtttattcc atgggatgat gatttagatt tctttatgcc tagagaggat
    25921 tatgaaaaat tttatcaact atgggttaat tcagaaaaaa gtaaaaaata tcccatacag
    25981 aaggcttcta aaaattataa tgatcataat tcttttacaa ctatccgcga tgcagatact
    26041 acatttatta aaacttatca aagagattta gatatagtcc atggaattac tattgatatt
    26101 ttcccgttag atgttgcccc tgtttcgaaa tttagccgta aaattcaaaa actatgggct
    26161 ttagtatatg ctttattttg ttctcaagtc gtccctgaaa aacatggtgg aataatggct
    26221 acaggtagta agatactctt atctattttc cgctcacctc gtacgagata taagatatgg
    26281 tcttttgctg aaaaacaaat gacaaaatat aataatgaac caacgggcta tgtgactgaa
    26341 ctttgtgtcg gacctaaata catgggaaat gtttatcctg caaaagactt tgaatctgct
    26401 gtgtgggtgc cattcgaaga tacagagatg ccagtaccag ttggttatga taattattta
    26461 agtcaagttt ttggtgacta tatgcagctt ccgcctgaaa aagatcaggt ttctcatcat
    26521 gaagcagttt atattgatcc agaacatcca tacacaatgt acaaaggaaa atattattta
    26581 actaaaggag cagaaaaaaa atgattacag ctattattat tgctggtggt gtgggtaaac
    26641 gaatgggaca agatatccca aaacagttca ttacaattga aggaaaacct attattatct
    26701 atactttaga atcatttcaa caccatcctc aagttgaccg gattttagtc gtttgcaaat
    26761 ctggttggga agaaacgatg tgggcgtata tcaaagaatt caatattagc aaagtcaaat
    26821 gggtcattac aggtggatca aagggacaag agtcgatcaa taatggtgtt cagtttttga
    26881 aagactattc tgaagatgat gatacgatca tcatccatga tggaatccgt ccactagtag
    26941 acgaactggt attgtcagat gtaatcgtta aatgtaaaga atttggtaac ggtgtaacct
    27001 ctctacctta caacgaacaa atttttatca agaaaacaga agaaacgacg gaacagtatg
    27061 tcgatcgtaa cacattgagg cgtgtctcaa ctccacaagc ttatcaatac ggaaaactac
    27121 gggcagcata tgaccgggca gtgaaagaag acatcggtat gactgattct tcttatacga
    27181 ataccatgat ggtagatttg ggagaaactt tatattttgc tgctggttct gataaaaata
    27241 tcaaattgac aacaacagat gatctagaat tgttcaaagc atatttaaaa atgaaagact
    27301 aggtgaaaga tgatgacgtt gattaaacaa ccaatgtacc aagaagattt ggcaaaagta
    27361 atcaatcata caccaaaaat aaaacagcta catggaaaat ccgttctgat gattggtgct
    27421 tctggaatga tcggctcttt tttgattgat acgttgatgg ctgctaatga acaactaggc
    27481 atcaaaatca aagtttatgc aatgggcaga aaccgtcaga agcttgaaaa aaggtttgcc
    27541 agttatcttg aattagatac tttcgaaatt gttgaaggtg acgtcacaga acccttacca
    27601 aaagaaattc aagctgacta tttgatacac ggtgcaagta acacacatcc taaggcttac
    27661 gcaactgatc caatcggtac gatcatgact aatcttgcag gtacagagca agtattgaaa
    27721 catgcagtag caacccaacc ggttagagta ttatttttat caacagtaga aatctatggc
    27781 gaaaatcgag gcgatataga aaaattcaca gaagattact gtggatacat cgattgtaat
    27841 acactgagag caggctaccc tgaaggaaaa agagtcagtg aatcattatg ccaagcctac
    27901 atcgcaaaac acgacctcga catcgtgatt ccaaggcttt gccgtacgtt tggtccgacg
    27961 atgcttttat ctgatacgaa agcatcctca caatttattt taaatgcagt cgaacaaaaa
    28021 gatattgttt taaaaagtga aggaaaccaa tatttttcgt atgtatatgt tggagatgct
    28081 gtttctgcta ttcttcattt actgcttgaa ggagaaaaag gagaggcgta caacgtttct
    28141 tcagaaaact ttgatttacg actaaaagaa ttagcccaac agctagctga tatcagcggc
    28201 aaacaagtaa tttttgaatt gccggatgag attgaacaaa aaggtttctc aaaagctagc
    28261 acagcagtac ttgaaaataa aaaaattaaa gagacaggtt ggaaaccaat ttttcctttg
    28321 aacgaatcat tggttcatac agtagaatta gttaaaggtg agaaatataa tgtgtgagat
    28381 tagtattatt gttcctgtgt ataaagttga aaaatattta agaaaatgtg tagattcgat
    28441 tttagcccaa acatttactg attttgaagt tatattagta gatgatggat ctcctgacaa
    28501 tagcgggaaa atctgtgatg agtatgctga aaaagataat cgagtacgtg tgatccat
//
LOCUS       DACDRN010000029        28333 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDRN010000029 DACDRN010000000
VERSION     DACDRN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.029"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            8..81
                     /locus_tag="IWJ80_001507"
     tRNA            8..81
                     /locus_tag="IWJ80_001507"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="IWJ80_001508"
     tRNA            101..173
                     /locus_tag="IWJ80_001508"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="IWJ80_001509"
     tRNA            201..274
                     /locus_tag="IWJ80_001509"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="IWJ80_001510"
     tRNA            288..361
                     /locus_tag="IWJ80_001510"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="IWJ80_001511"
     tRNA            385..474
                     /locus_tag="IWJ80_001511"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="IWJ80_001512"
     tRNA            487..560
                     /locus_tag="IWJ80_001512"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="IWJ80_001513"
     tRNA            579..654
                     /locus_tag="IWJ80_001513"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="IWJ80_001514"
     tRNA            656..726
                     /locus_tag="IWJ80_001514"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="IWJ80_001515"
     tRNA            740..813
                     /locus_tag="IWJ80_001515"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="IWJ80_001516"
     tRNA            838..925
                     /locus_tag="IWJ80_001516"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="IWJ80_001517"
     tRNA            947..1018
                     /locus_tag="IWJ80_001517"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="IWJ80_001518"
     tRNA            1041..1130
                     /locus_tag="IWJ80_001518"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="IWJ80_001519"
     tRNA            1143..1216
                     /locus_tag="IWJ80_001519"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="IWJ80_001520"
     tRNA            1235..1307
                     /locus_tag="IWJ80_001520"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="IWJ80_001521"
     tRNA            1311..1383
                     /locus_tag="IWJ80_001521"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="IWJ80_001522"
     tRNA            1388..1458
                     /locus_tag="IWJ80_001522"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="IWJ80_001523"
     tRNA            1471..1544
                     /locus_tag="IWJ80_001523"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="IWJ80_001524"
     tRNA            1556..1627
                     /locus_tag="IWJ80_001524"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     gene            1893..3086
                     /locus_tag="IWJ80_001525"
     CDS             1893..3086
                     /locus_tag="IWJ80_001525"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP9461245.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="IWJ80_001526"
     CDS             3216..4688
                     /locus_tag="IWJ80_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9461246.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="IWJ80_001527"
     CDS             4771..5559
                     /locus_tag="IWJ80_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP9461247.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="IWJ80_001528"
     CDS             complement(5626..6294)
                     /locus_tag="IWJ80_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9461248.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGIKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="IWJ80_001529"
     CDS             6409..8121
                     /locus_tag="IWJ80_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9461249.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9893
                     /locus_tag="IWJ80_001530"
     CDS             8124..9893
                     /locus_tag="IWJ80_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9461250.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10098..10664)
                     /locus_tag="IWJ80_001531"
     CDS             complement(10098..10664)
                     /locus_tag="IWJ80_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9461251.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(10664..11620)
                     /locus_tag="IWJ80_001532"
     CDS             complement(10664..11620)
                     /locus_tag="IWJ80_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP9461252.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(11620..12276)
                     /locus_tag="IWJ80_001533"
     CDS             complement(11620..12276)
                     /locus_tag="IWJ80_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9461253.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            12646..14664
                     /locus_tag="IWJ80_001534"
     CDS             12646..14664
                     /locus_tag="IWJ80_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9461254.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFSARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            15091..17505
                     /locus_tag="IWJ80_001535"
     CDS             15091..17505
                     /locus_tag="IWJ80_001535"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP9461255.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            17853..19406
                     /locus_tag="IWJ80_001536"
     CDS             17853..19406
                     /locus_tag="IWJ80_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9461256.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(19787..21571)
                     /locus_tag="IWJ80_001537"
     CDS             complement(19787..21571)
                     /locus_tag="IWJ80_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9461257.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(21586..23307)
                     /locus_tag="IWJ80_001538"
     CDS             complement(21586..23307)
                     /locus_tag="IWJ80_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9461258.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            23407..24168
                     /locus_tag="IWJ80_001539"
     CDS             23407..24168
                     /locus_tag="IWJ80_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9461259.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            25135..25403
                     /locus_tag="IWJ80_001540"
                     /pseudo
     CDS             25135..25403
                     /locus_tag="IWJ80_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            26075..>28333
                     /locus_tag="IWJ80_001541"
     CDS             26075..>28333
                     /locus_tag="IWJ80_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461260.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKL"
BASE COUNT         8937 a         5061 c         5996 g         8339 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaattccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa taaagaggtt
     9901 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag cttctcatgt
     9961 ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt gaataaagag
    10021 gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac atatcattta
    10081 tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg cggctggttc
    10141 ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag tttggaacaa
    10201 aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta gacgacaagg
    10261 atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt gcgtgtagtc
    10321 tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt gaaaatccct
    10381 gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc ttgtagaaac
    10441 aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc atcgattgct
    10501 tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc tgctagaaaa
    10561 accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg ttgcaagatt
    10621 tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg ctcacctttt
    10681 tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca tcaattgcat
    10741 tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc cacggcgcat
    10801 cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg tcgcaaatga
    10861 cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag tcccgcaaca
    10921 ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata tctgaatcta
    10981 aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt aatccgttga
    11041 tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca tctaaataag
    11101 tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt gtttgcaatc
    11161 caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca actacttcat
    11221 ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt aaattgacta
    11281 cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta aaattttgga
    11341 aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg atttctttct
    11401 ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa ccagatacac
    11461 tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag tcaaatcctc
    11521 catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg taattccaag
    11581 aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct tctttctgta
    11641 agagttatct atttgttttc ttttttctct atacactgga taagtcaggc acagccaaga
    11701 aagactgagt aagtagccgc cgatgatatc agtaggaaaa tgaacgccca gataaatgcg
    11761 gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg tgcgtaaggt
    11821 tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa tcatactgcc
    11881 tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg ccaatgaagg
    11941 acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa ttacaataaa
    12001 tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc ataaagccgc
    12061 acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa agagttggta
    12121 gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat cgaaaccaga
    12181 aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga caagaaagaa
    12241 gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt gaagcaatga
    12301 cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac caatgtatga
    12361 ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga aagacatttt
    12421 ttggtaatgc aaatgaaatg ttagtgaaat aaaatcagtg tttttcgaaa tccttttaaa
    12481 caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt acggtaaatt
    12541 acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat attaagagaa
    12601 gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga aaacattgca
    12661 agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa acgaggggca
    12721 gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt acagccggtt
    12781 cctgttcaag cggatgaaac acctgttcag ttcagcgcaa gaatcaatac agcggcattt
    12841 atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct atatgcatca
    12901 gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt atctcaagca
    12961 ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt ctacatggat
    13021 acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg acaatatcct
    13081 tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc atttggcaac
    13141 ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga tgcgacagct
    13201 tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa taatatcatc
    13261 gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg cggtggagcg
    13321 actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag caattctggt
    13381 aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg tgattctgtt
    13441 tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg gaacaacatc
    13501 caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca ggaaaatggc
    13561 tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg tacatcaaac
    13621 ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt ttggaagatc
    13681 tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat caagaacaat
    13741 ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa tgctggagca
    13801 aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc aagttcttct
    13861 aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg gagcgtctca
    13921 aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca aaataacttt
    13981 atctacccag gacaacaact gactgtttca aacagtggat caacaaacag cacgtcaggt
    14041 aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg cagcagttcg
    14101 tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc aaataaaaac
    14161 ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt catttatcca
    14221 ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg ttcaactact
    14281 tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc atccactggt
    14341 aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa caaacatcat
    14401 atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat ctatccagga
    14461 cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc agttactggt
    14521 tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga cagtcatgga
    14581 attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat ttatccaggt
    14641 caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt gaattccaca
    14701 aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg aagtagtaaa
    14761 aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc ttttgaactc
    14821 gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg gccgttacaa
    14881 cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta cgtcctataa
    14941 aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag aaaacaaagc
    15001 aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat aatagaaaca
    15061 agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga aattgagaaa
    15121 aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga tcctgaaaag
    15181 ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct acatgtaggg
    15241 catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag tcaaggatac
    15301 aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca atatgcgcta
    15361 gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt ccgacgccaa
    15421 atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac tgatccggag
    15481 tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct agcttatgaa
    15541 gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa tgaagaagtg
    15601 atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat gcgccaatgg
    15661 atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt agttgattgg
    15721 ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg agcaaatatt
    15781 gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg accagatact
    15841 ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag agaaattacg
    15901 acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa aaaatcagat
    15961 ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc ttatgcaatc
    16021 aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt atcttcatac
    16081 ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga attcgctcaa
    16141 actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa agctgcttac
    16201 acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa acaagaagca
    16261 atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga agtcagctat
    16321 cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat cccaatcatc
    16381 cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt acggttgcct
    16441 aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa tatcgaggag
    16501 tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac gaatacaatg
    16561 ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca taacaaaaaa
    16621 gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta cattggtgga
    16681 gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt cttatacgat
    16741 atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg aatgatctta
    16801 ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc tgatgatgtg
    16861 gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg gccattagat
    16921 gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt ggatcgtgta
    16981 tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac attgaatgat
    17041 ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga ctatgaaaat
    17101 cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc ctataaagtg
    17161 gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc gattgctcct
    17221 cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc ttacgcacca
    17281 tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt tttccaagta
    17341 aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga tgagctagaa
    17401 aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac agtcagaaaa
    17461 gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc tctttgtcaa
    17521 taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca ttctgtctca
    17581 gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga ttttcatgtt
    17641 ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct ctgtaatcat
    17701 caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga aattgagagt
    17761 acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga ttttgacgca
    17821 taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt attatcgaag
    17881 tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa agaataatgt
    17941 tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct gtatcgttcc
    18001 tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa ttaaataatt
    18061 tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt gtttttcttc
    18121 tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg cttagtcaaa
    18181 gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat ctttattttg
    18241 agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag ttattgctga
    18301 gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc ggaatgaaca
    18361 cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag tcttcgcctg
    18421 gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc attcttttag
    18481 ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat ataaatgctg
    18541 cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca aatgaagggt
    18601 tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat gatctcaaac
    18661 ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat tgggtatttg
    18721 tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg gggctgattc
    18781 ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca tggtttatct
    18841 attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa acatggcaaa
    18901 cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta ttttatggca
    18961 acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact ttgtacattt
    19021 acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg atcaatcagc
    19081 cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca cgtgaaagtt
    19141 tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg attccggaaa
    19201 actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact caaataagcg
    19261 cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat gcagggaaaa
    19321 tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc gttcagcaac
    19381 tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg ttcagcaccg
    19441 agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt gtaactcgtg
    19501 attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc gtattttaca
    19561 agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt aaaaagggat
    19621 tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc caaaaagtat
    19681 cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa aagctatcga
    19741 tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac gctatctgaa
    19801 attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca gaatgtgttc
    19861 ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt tcaatcgttg
    19921 acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc attgcttgct
    19981 gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa ataacgacag
    20041 gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct tgagataacg
    20101 tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga ataaagtgat
    20161 cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc attttcccgt
    20221 aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa accatcccaa
    20281 aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg aattcgatct
    20341 ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt tttcctgcac
    20401 cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg acttgattca
    20461 tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt tttcctttcg
    20521 gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca tctaatatct
    20581 caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca taagaagcgg
    20641 tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag aagccgatcg
    20701 tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg aaggcgagtt
    20761 gattaatgaa acgaatggcc ggatagatag caaagttcat aaattgagca cgtttaaaag
    20821 cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct tgctgattga
    20881 atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt aaaacaccca
    20941 gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta gttgtaacaa
    21001 ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg atataaaaca
    21061 gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg atacctgtca
    21121 ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg tcacctactt
    21181 gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt tcttttcgca
    21241 ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata aatgaagtga
    21301 tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat gcttgtttga
    21361 tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag agatcatcga
    21421 tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat aaactgcata
    21481 gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa cgtatcaaag
    21541 cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata ttccttgtga
    21601 ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag taccttgtcc
    21661 aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg tactgaggcg
    21721 ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg ctcttaatgc
    21781 tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga tctctgctgg
    21841 tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat aattagaacc
    21901 gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt cttctaattg
    21961 agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc ccatctgcaa
    22021 gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac tgatgtggga
    22081 gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta cttttcctat
    22141 cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag atccggtccc
    22201 gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt ctagaactgg
    22261 atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat ttgtctgatc
    22321 aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct ctgcttgcag
    22381 tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca ataccatagc
    22441 tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag tcacgacacc
    22501 gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag aagcataaac
    22561 gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt tcactttgat
    22621 catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat atgattgatt
    22681 atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga tggtgatccg
    22741 atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca gtcgataaac
    22801 aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata gagaataacg
    22861 aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca tttggcaaaa
    22921 taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca aaagagatcc
    22981 agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga aatatttctc
    23041 ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag aggcaactgc
    23101 agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt ttataactga
    23161 ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg gtacacctaa
    23221 tgtaccggcg atttgtaaac agatcatcac aaaggtaagg agagcgagtc ctttgttttt
    23281 ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat acttttcgat
    23341 tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa aaagaggtga
    23401 gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga tttgccaaaa
    23461 tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca agaagagaat
    23521 gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt tttttataga
    23581 aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt gacggagtta
    23641 tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt gatgaaacaa
    23701 aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga tacaaacgag
    23761 gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga gtttgaagat
    23821 attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat gagtatgcca
    23881 gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt acatttattt
    23941 actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt cttattgaaa
    24001 tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag aatgtatgaa
    24061 aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac ggatgaaaac
    24121 aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga aatatgtgtt
    24181 taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct ggttcgatta
    24241 cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt tttagaggaa
    24301 atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg taattgtcct
    24361 cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat ggtgaactga
    24421 gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat gagaatgata
    24481 aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac attattacaa
    24541 tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt aatcaaatag
    24601 tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt tttgcttata
    24661 aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag aaaaatcaca
    24721 aaggttattg ttatttttat atattctttt taagaatgat tgttttttta tagaaatttt
    24781 aaaaaataaa acacgttatt gatggatagt tagtttgtta tttggtaata ttatagaagt
    24841 tgactggtag tgatttattt ttcgtcgttt tataaaaatg ttattttttg gaaaaattca
    24901 caaatatgta acgaatgttt ttctggaatt tgctgtgtta aaaagcagta tgactaatat
    24961 aaatactatt gtcatttatg caatttttta tggatagtaa ttttagaggg gaatcaatga
    25021 aggaagaata gaagggaaaa atagttgcaa atacgccagt tagcaaaaat agcaatacat
    25081 cttcaagctt taaaattcga gagaatcatc ttggtagtgg aatcaactac aacaaaagga
    25141 ttaaaccaat taatagatag aattaccaat ggaaaaatag aaaaggtagt tattctatac
    25201 aaaagttgtt taattcgctt cggttatgaa cttagtgaaa ccctttgcaa tatatggtac
    25261 agaatataga aattattgac attacagaaa atgatgaaga tttaatacag attgtcacta
    25321 tttgtagata ccttttacaa ggaaaccgag ccaataaagc taagaagatg attaaggagt
    25381 tgatggaaga cgatacaagt taagaaagtg aaactacgcc caaataaaga gcaaaaagag
    25441 taattggacg tggaaaatgc aagaaatgaa ccatcgattc ggtggacagt ttattagtaa
    25501 tgacggtttg agaacatcca tgacgagaat gaaggaacat cctaaatgcg tttagttgaa
    25561 ccaagtgcca aataatatgg ccaaataagc gatcagttat cgatgaatcc caagtattct
    25621 aaactatata ttcattttga tgctaagcat tgatatattt ttttgggatt gatgaaggtt
    25681 aaccaataca gggactcact gatgaaattc tagagtctga ccttggtatc aacgatttag
    25741 tgattgtttc taatggaaaa gtataaaagt atataagtac attaacaaaa taactattag
    25801 ggttacgctc cagcttgtgc aaggcactgg caggaggaat cgtgaaatca taattatggt
    25861 taagccacac ggacaactaa aagtcataca aagctatcga atcaatcaac agaagacaat
    25921 cggcaatcat tagctttgtt tacagcttac attgcaacgc ttgagttgaa ccgaaaatac
    25981 gagggaggta acgcagccat tttgagttgg ggaatctttt tagaaaatta tcgggtacct
    26041 tgttgtttag tcaaaattat aagggggagt aatagtgagg aacaaatttc ataaactgtt
    26101 aagtatgttt ctagttatgc ttattgtgat caatcaattt gggaacataa cggttctagc
    26161 tgagtcttta gcagaacagc cagagcgttc gctgacgatc gaagttgatc aagaggaggc
    26221 tgctttgggt gagcagttcc agttgcgagt cagaggaaca ccagacctgc taaatgcatt
    26281 ggtcattgat aagagtgagg atttgattga aggggaagtg aagcaactca atgattacga
    26341 acgtctgatt acttattctt cccagcattc aggtaaatac gaattgaccg caagaagcgg
    26401 tgatattcaa tcaaatacgg tctcaataga aatcagtgta ccagaagaaa gcacaagtga
    26461 gtcttttaat gtaccagaag aaagcacaag tgaatctttt aatataccag aagaaagctt
    26521 aagtgaatct tttaatgaag ccagatcatc tgagccatct ataacgcagg aatctcagat
    26581 tgaaagacaa gaggagctct tgaattatga catcggcatc tctgctgagc gccccacttt
    26641 gccgagcagg catacacaaa cgttaaaggg atacattcaa ggaggcgctg gcgatattct
    26701 tgaagaatat ggggagatgg tcgtacgagt tcctagagca tcgattggct taattgagtt
    26761 cattagagtg gaggtcgctg atgattcacc tttaaagcca ctggcatttt accaagaagg
    26821 cacagacgtt gtttttccat ttacctttga ttatgaagga ttaaatgaag ccgctcagtc
    26881 gtatacattc agtatcacgt ttgaagttgc tttctttcaa tcaggcggta ctgagccaaa
    26941 taatgacttt caagtgagtc ttacttatgc tgagcgagca gcaaacacgg aattttcaat
    27001 tacgcctatt ccttcatcaa gacccatttt ttcgaaatta tactatggct ctagagaaat
    27061 cgacagccaa ggaaatcttg ggaatctgct gattgacggg caagacgtga ccaacagtaa
    27121 taaatttatt ttgcaggtaa attatgaagg atcacgaaaa gacggtgtag tggttaccga
    27181 tgtactaccg caaggaacaa ctttggcacc gaatccgact acatggagtt ccactggaaa
    27241 tagagaacct atttcaggtg gggttcggat cgcagaagta acgaatccag gtgaagcaaa
    27301 cgttagaact cattatgttt ccgaggaatt tgtggacaaa gtatctttta ataaggaaac
    27361 gaatacactt gttgtggact ttggtaatct agcagagggt caatcgtatt ttattgaata
    27421 tgctcttgaa gtgaccgatg tcgacttgtt caatgaagga agacaagagg atgggttttc
    27481 tcggaatcga gcgagtatga cctacaataa tcaggtaatg agccgagact accgcttgag
    27541 aatccctgaa agtaatcttc cggcaatctc tttaagaaaa agtgtggaca aaaatgttat
    27601 caatctcaat gaaaatgaac taacgtatac tctgcatgtc tcgatgtttt ctggaacaag
    27661 aaatacgctt gttattgaag atgttttgga agaaggcatg gaatttgtgt catttctatc
    27721 agatattccc tctttcattg atgccgaagt caataatgga atagttcgtt tcagtgcgaa
    27781 taatacgatt ggtggtccga caagttttac cctctctttt aaggtcaatg tagaggcgta
    27841 tgcggtcggc gatcaaattt taaactttgc aacgcttgtg aatagtgaca atcgattgaa
    27901 tacgagtact gtaagaacaa ggaaaatcga tgggcgtatt aaagtaatta aaactgatga
    27961 gaatggccaa aggctggcag gagcaaaatt tgaaatttta tcaagcgatt cacaagtgat
    28021 acaagaaggt ataacggcag aaaacggaga atttttaagt gaaccgcttc ctgtaggtag
    28081 ctatcaagtc cgagaaacag aagcaccgga agggtatctt ttagatgcga cggtacatga
    28141 tgtttttata aatgaatact ccatagaaga agtgacgtta accattgaaa acatccttga
    28201 tacgggtggt gttgaactaa ttaaacatgc tgaaggcagt ggtgaagtcc ttcaaggagc
    28261 tgtttttgag ttacagaacc gagaaggaga aacgctacaa accggtttaa ctacaggaga
    28321 agatggaaaa ctg
//
LOCUS       DACDRN010000030        27560 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDRN010000030 DACDRN010000000
VERSION     DACDRN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.030"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            188..418
                     /locus_tag="IWJ80_001542"
     CDS             188..418
                     /locus_tag="IWJ80_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP9461261.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            515..1816
                     /gene="murA"
                     /locus_tag="IWJ80_001543"
     CDS             515..1816
                     /gene="murA"
                     /locus_tag="IWJ80_001543"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9461262.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1829..2005
                     /locus_tag="IWJ80_001544"
     CDS             1829..2005
                     /locus_tag="IWJ80_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9461263.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2140..2436
                     /gene="yidD"
                     /locus_tag="IWJ80_001545"
     CDS             2140..2436
                     /gene="yidD"
                     /locus_tag="IWJ80_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP9461264.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2621..3088
                     /locus_tag="IWJ80_001546"
     CDS             2621..3088
                     /locus_tag="IWJ80_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9461265.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3173..4057
                     /locus_tag="IWJ80_001547"
     CDS             3173..4057
                     /locus_tag="IWJ80_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9461266.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4174..4407
                     /locus_tag="IWJ80_001548"
     CDS             4174..4407
                     /locus_tag="IWJ80_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP9461267.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4495..4575
                     /locus_tag="IWJ80_001549"
     tRNA            4495..4575
                     /locus_tag="IWJ80_001549"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4529..4531,aa:Tyr,seq:gta)
     gene            4582..4653
                     /locus_tag="IWJ80_001550"
     tRNA            4582..4653
                     /locus_tag="IWJ80_001550"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4614..4616,aa:Gln,seq:ttg)
     gene            5168..5875
                     /locus_tag="IWJ80_001551"
     CDS             5168..5875
                     /locus_tag="IWJ80_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9461268.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5934..6578)
                     /locus_tag="IWJ80_001552"
     CDS             complement(5934..6578)
                     /locus_tag="IWJ80_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP9461269.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6704..6991
                     /locus_tag="IWJ80_001553"
     CDS             6704..6991
                     /locus_tag="IWJ80_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461270.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7099..7419
                     /locus_tag="IWJ80_001554"
     CDS             7099..7419
                     /locus_tag="IWJ80_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9461271.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7500..8813
                     /locus_tag="IWJ80_001555"
     CDS             7500..8813
                     /locus_tag="IWJ80_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9461272.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8810..9529
                     /locus_tag="IWJ80_001556"
     CDS             8810..9529
                     /locus_tag="IWJ80_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP9461273.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9657..10208
                     /gene="raiA"
                     /locus_tag="IWJ80_001557"
     CDS             9657..10208
                     /gene="raiA"
                     /locus_tag="IWJ80_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP9461274.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10430..12964
                     /gene="secA"
                     /locus_tag="IWJ80_001558"
     CDS             10430..12964
                     /gene="secA"
                     /locus_tag="IWJ80_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP9461275.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13090..14191
                     /gene="prfB"
                     /locus_tag="IWJ80_001559"
     CDS             join(13090..13152,13154..14191)
                     /gene="prfB"
                     /locus_tag="IWJ80_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP9461276.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14296..14982
                     /gene="ftsE"
                     /locus_tag="IWJ80_001560"
     CDS             14296..14982
                     /gene="ftsE"
                     /locus_tag="IWJ80_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP9461277.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14966..15859
                     /locus_tag="IWJ80_001561"
     CDS             14966..15859
                     /locus_tag="IWJ80_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9461278.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            16011..17459
                     /gene="cls"
                     /locus_tag="IWJ80_001562"
     CDS             16011..17459
                     /gene="cls"
                     /locus_tag="IWJ80_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9461279.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17796..18653
                     /locus_tag="IWJ80_001563"
     CDS             17796..18653
                     /locus_tag="IWJ80_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP9461280.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18768..19688
                     /gene="pstC"
                     /locus_tag="IWJ80_001564"
     CDS             18768..19688
                     /gene="pstC"
                     /locus_tag="IWJ80_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP9461281.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19688..20572
                     /gene="pstA"
                     /locus_tag="IWJ80_001565"
     CDS             19688..20572
                     /gene="pstA"
                     /locus_tag="IWJ80_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP9461282.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVSEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20587..21393
                     /gene="pstB"
                     /locus_tag="IWJ80_001566"
     CDS             20587..21393
                     /gene="pstB"
                     /locus_tag="IWJ80_001566"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9461283.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21409..22167
                     /locus_tag="IWJ80_001567"
     CDS             21409..22167
                     /locus_tag="IWJ80_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9461284.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKNKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22180..22857
                     /gene="phoU"
                     /locus_tag="IWJ80_001568"
     CDS             22180..22857
                     /gene="phoU"
                     /locus_tag="IWJ80_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP9461285.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23094..24125
                     /locus_tag="IWJ80_001569"
     CDS             23094..24125
                     /locus_tag="IWJ80_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9461286.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24122..24889
                     /locus_tag="IWJ80_001570"
     CDS             24122..24889
                     /locus_tag="IWJ80_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9461287.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24989..26557
                     /gene="liaX"
                     /locus_tag="IWJ80_001571"
     CDS             24989..26557
                     /gene="liaX"
                     /locus_tag="IWJ80_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP9461288.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26857..27174
                     /locus_tag="IWJ80_001572"
     CDS             26857..27174
                     /locus_tag="IWJ80_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP9461289.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27174..27524
                     /locus_tag="IWJ80_001573"
     CDS             27174..27524
                     /locus_tag="IWJ80_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP9461290.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
BASE COUNT         9096 a         4667 c         5958 g         7839 t
ORIGIN      
        1 tttggaataa gggactgcga caagactttt gtctcagtcc ctttttttgc atatttttag
       61 catttatttc ctaaaaaatg taaggggatt cctatatttt taagtatact tgtttttcct
      121 tttatacatt ttatggtatt atcattttat tagttattat aaaacaatcg ttagaaagga
      181 cgtcattatg caagtatacg gaattgatgc ggtgcttcga atcgtgagtc atttggcgtt
      241 tatctactta gccttctggt ctttacgttc gttacgcatt gaaacttttt tcagaccaat
      301 gcataatacg caaattagaa tggcaatcgt acttttttca atttttttag ggtacactgc
      361 aagtaatttt ttcttagaag ttatcgcttt atgccgaaat cttttcgtta gcctgtaaat
      421 tttaaaaaaa ggtactattt taaaagcaag gatgttataa tagaaaagat tttgagtaag
      481 tgtatgctca ttggaatttt ttggagggaa caacatggaa gagatcatcg taagaggtgg
      541 caatcaatta aatggaacag taagaattga aggagctaaa aatgctgttt tgcctatttt
      601 agctgctagt ttgttagcag aagaaggtat tacgacatta gacaacgttc cgattctttc
      661 tgatgtcttc actatgaatc aagtgattcg tcacttaaac gtagatgtag attttgatga
      721 acaaaagaat caagtaacaa tcgacgcttc aagacaatta gaaatcgaag cgccttatga
      781 gtatgttagt caaatgcgtg cttcaatcgt tgtgatgggt ccgctacttg ccagaaatgg
      841 acatgcaaaa gtagctatgc ctggtggatg tgcgattggt aaacgaccaa tcgatttgca
      901 tttaaaagga tttcaagctt taggagcaaa aattattcaa aaaaacggct atatcgaagc
      961 aattgcggat gaattgatcg gtaacacgat ttatttagat ttccctagtg taggtgctac
     1021 tcaaaatatc atgatggctg ccgtaaaagc aaaaggaaca accatcattg aaaacgtagc
     1081 tcgcgaacca gaaatcgttg atttagcaaa cattttgaat aaaatgggcg ctcaagtcta
     1141 tggagctggt actgaaacaa tgcgtattga aggcgtggat catttacatg cagtcaacca
     1201 ctcaatcgta caagatcgta tcgaagcagg tacctttatg gttgctgctg caatgacaca
     1261 aggcaatgtg ctgattgcag atgctatctc tgaacataac cgcccattga tttctaaatt
     1321 gatcgaaatg ggtgcagaaa tcattgaaga agaaggcgga gtccgtgtca tcggaccaaa
     1381 acatatctta cctacagatg taaaaacgat gcctcatcct ggtttcccta cagatatgca
     1441 agctcaaatg acagctatcc aattagttgc agaaggtaca agtgtagtga ctgaaacagt
     1501 gtttgaaaat cgtttccaac atttagaaga gatgcgccgt atgaacgctc atgtcaaaat
     1561 cgacggaaat gtagcaatca tggatggaaa ccatgaattg caaggtgcag aagtatatgc
     1621 aacagacttg cgggcagcag cagcattagt tttagctggc ttgaaagcaa atggaatcac
     1681 acgtgtgcgt aacttgaatt atctagaccg tggatattat aatttccata tcaaactaca
     1741 gcaattaggt gcagacgttg aacgtgtaga catggatcaa acttctgctg aaaaaacagc
     1801 acaaacgatt gcttaaggaa gtggctgaat gagtaaccaa cgatatatct tattaacttt
     1861 gataaagatt ttaatggtga tcttattgct tatccttctt tttgtggccg gaacgatgat
     1921 cggatatggt gtcatcggtg gcggcaatcc atttaaagta tttcagccaa gtttgtggat
     1981 tcatattcga gacttttttc attgataaat aagaatcaca gtggttctag tcgatatgaa
     2041 gtgagccatg aagcagaaca tgataaaaga aaacagaagg gaacgttgac gttagtcgcg
     2101 atcccttttt ttagtaaaag aaaagaagga atctaattga tgaagaagat attgatttat
     2161 ttagttagag gatatcagcg ctttatttcg cctgcatttc cacctagctg ccgttattac
     2221 ccaacctgtt ccaattatat gataaaagca attcaaaccc atggcgcgct aaaagggacg
     2281 gtaatgggga ctgcacggat cttacgctgt catccttttg tcaaaggcgg tatcgattat
     2341 gtaccattga cattccgatt aacgcgaaat aagaaagata aaaaagaacc agattataaa
     2401 aaggttgtga aaaatacggg tagcactgag cgataaagaa acaatttgga aaatagcttc
     2461 ttatattttg caaaattgtg agttattgcc gaagtgttgg attttgaaca ccgtcaaaaa
     2521 aaaggttgtg aaaaatacgg ctagcactga gcgataaaga aaaagaggaa atttattgtc
     2581 aaagtaacaa ctccgcaact acttatatgg gggacaaaaa atgatcgaag aactaaacaa
     2641 gaaagattta ccatggtcct tgttattaga tgcagatcct gataaagaaa aagttcaagt
     2701 atatgtttcc agagggagtg gctggatatg gaaagaaaaa gaaaaaacaa taggtgtact
     2761 gatctatgtg gcaagggaaa aagaatacga aatcgtcaat gtagcagtaa ccccaacgta
     2821 tcaaggaaag gggattggcg gaaaattact tgagacagct tttcagaagc tttcacaact
     2881 cgcttccagt caaactcaga tcatcatacg aaccggcagt actagcagtg cggcgttaca
     2941 tctctatcaa aaaatgggct atgtggagat tggtcgagtc aaagactatt ttatccatca
     3001 ttactctgag cccatttttg aaaatggaga tcaattaagg gatcaagtga ttttagcaat
     3061 tcacttacct aaaaatagct ttaactaata aaatttcaat tcaagaaaat gctttcaaat
     3121 gctaaataga tacattacaa tagctttagc aaacaaaaag gagaatattt ttatgggaat
     3181 attagttttt gattttggtg gatcggctgt caaatatggt tgttgggatg gcaaagagat
     3241 taaaggtaag ggacaatttg caacgcctga atcttgggaa gaaatgaaag cgcaattatt
     3301 tcaagtttat aaaaaaatac tcgtttcttt tgaagtcgaa ggaattggaa tcagctctcc
     3361 tggagtagtg aataatgaga aacaggtgat tgagggaatc agtgcgattc cttatattca
     3421 tggatttaat attttccgag atttggaaac tctgtttcaa cttccagtaa cgattgaaaa
     3481 tgacgccaac tgtgccggaa tggctgaatt ttatgaaggt gctgcccgcg attatcaaga
     3541 tgttgctttt gttgtggcag gaactgggat tggcggggcg ttgttcacga aaggacaaat
     3601 caataaaggg gcacatcttt acggtggaga atttgggttg atgttcttaa atggcgataa
     3661 gacattcagt aaacttggta cagcggtaca gatggcttgg cgttattgtg aacgcaaagg
     3721 tctcgataaa aatgcttata ccggaaaaga tgtctttgaa ttagcagaaa ctggagatgc
     3781 aatagcaaaa gaagaagttg agagttttta tacgtatctt acaaagggat tattcagtat
     3841 tcaattttcg tttgatccag aagtgatcgt attaggcggc ggggtttctg caaaagaagg
     3901 gctcattgat gagatcaaaa gccgaatgaa gaagctgaca gaacaattcg atctgcatga
     3961 tttcgaaccg cagattttat tatgtgaata cagaaacgat gctaatcttg taggtgcagc
     4021 agcaaactat ctcgctgtac gtcaagggga gaattaagag ctttagagaa ggtgcagctt
     4081 gcataaaaga agtgaatgaa atgaatagga gatttttacc taaggaatgt tttatttgct
     4141 gcagtatgat aaaatatctg agaggtgaaa atgatgtcaa aaaaaaataa agatattgaa
     4201 gtgcgtatag aagaagtgaa aaaagatatc aaaggtaaga attatgaagt cactcagtta
     4261 tttataggga aaaaaatgat tggtgagatc ttagcctatg gaccaaaaga atatgaaatt
     4321 ttctttggtg aagaagattt tggaaaagaa aaaagtcttg aaaatgcgat agaaacagtg
     4381 attcgacact ggaatctgca cgaataaaaa aatatcaaaa aaactttcaa taaagggttg
     4441 caaaaatctg gaaaattagg tattataact aagtcaggtt gatacaaacc tttcggagga
     4501 gtagcgaagt ggctaaacgc gacggactgt aaatccgttc cttagggttc agtggttcga
     4561 atccactctc ctccatttca ttggggtata gccaagcggt aaggcaaggg actttgactc
     4621 cctcatgcgt tggttcgaat ccagctaccc cagtatacca caatcgtaag ctctcgttag
     4681 ttcgagagct cttttttttg ctttgctacc ttgttccttg aagattctgc tgatttaaga
     4741 tagagttaag ttaactacaa gaattggagg gatccacatg aaatggaaag aaaaagtaga
     4801 cgaagcagct gaaaagttat atgatttggt caaatcagaa aaatacaata ttgaagtgaa
     4861 tattccgaaa aaaggcggaa aagcggtccg ggtaaaatca aaacgcccaa cgaatcatac
     4921 aaaaaaatgg atggctaaaa accgctaata ataacaaaag atccataagt cggcattttc
     4981 atcgatttta gggtcttttg ttattttcta ggttggacat atctaaaaaa ttgtttctga
     5041 caataggagt aggaagaacg atgtgtgtct aaatcctctg ttgatggata gaaaccttta
     5101 tctttttcag ttgtgaatca ggtatatacc agttataata gaagaaacga aaggacgggt
     5161 gtacgttatg tcgaatcaat tacctgtata tatacagatt catgaccaat taaaaaatga
     5221 aatcgaacaa ggtatatgga aagtcggaga ccgtcttcct tcagaaagag aattagcagt
     5281 gaaattcaat gtcagccgta tgacgttgag acaagcaatt caaaacttgg cagatgaagg
     5341 aatcttagaa agaaagatcg gttcgggtac atatgttgca cgagaaaaag tacaagaaaa
     5401 aatgtctggt gcaacaagtt ttacagaaat tatggaatca caaaatcgag taccgtctag
     5461 tcggacaatc tcttatttct tgacttcgcc aagctcaagt gaaatggaaa aactaaactt
     5521 gagtaaagat gacacgatct tgcgaatgga acgtattcgt ttcgcagatg atgtccctat
     5581 ttgttttgaa gttgccagtg tgccacaaaa gctgatcgat ggctacagca aagcagagat
     5641 tactgaatcg ttgtatcgca cgctagaaga aaaaggcggg aaaaagatcg gtccagcgaa
     5701 tcaaacgatt actgctgtgt tggcttctga gaaaattgct gactatttag aggtaaaacg
     5761 aggagatgca gttcttcgtt tgcgccagat ttcttatttt gaagacggta cgccgtttga
     5821 gtatgtgcgt acgcaatacg taggaaatcg ttttgagttt tatttggaaa aataaaaaac
     5881 aggtgatccc tgattccgta ttagaaataa aagagaatca gaatcacctg tttttatata
     5941 tgatcagtta ccagttgctc gtgataagaa acaggaccct cttcaaattg gacttgtccg
     6001 ttcagcagct caatgatgga ttctttaaag tggtcagcac ttttttcatc tacagcacag
     6061 atgacgctaa cttgatcagt atattggatt tcttttataa aaaaatgttg gagttcaaga
     6121 taattctgta attttcctag ttgcggatac gtgatcatca cagagatctc ctgttgcaag
     6181 gtaccttcta cgattccaat gtcagacaat gcttgagaga ccgcatgagt gtatgctcgt
     6241 attaatccgc ctgtgccgag tttcgttcct ccaaaatatc gagtgactac tgcagctaca
     6301 ttgatcagtt gatttttttt gaggacttcc aacatcggta tgcccgccgt tccacttggt
     6361 tctccatcat cgctgcttcg ctggatttca tttttttctc caatgatgaa ggcgctgcag
     6421 ttatggttcg ctttccaatg ttcttttttt atttcttgaa tgaaactttt tgcttcgtcc
     6481 tctgattcta ttcgtctaag agaacagata aagcgtgatt ttttgatctc gatctcactt
     6541 tgtccatttt cttttattgt acgatagcgc tcgatcatct tgtcacctct tttttcattt
     6601 tctttattca gtataaataa agttctaggt tgtttcaaca ctgtaacggt tgcgcaattg
     6661 ctgtagtatg cgataataaa gacaaaggca ggtggatctg gggatgcagg aacgaatgaa
     6721 aaagtacgat gcaattacgc attatttgaa gaacaatggc ggctctcaag taacgttgac
     6781 ctttacccaa tttgacgaac tgctgtttcc cagtaacgga cttcccaaaa cagcaaggga
     6841 aagtacagat tggtgggcaa atgattataa acaccctgaa aaaggtgcct atggctggat
     6901 aaatgcaggc tatgaagtag ttgtcatcaa tttggacaaa gaatatgtcg tgttcaataa
     6961 attagtgaaa tctagttggc tatttgatta aataaaccgc gtttagaaaa cgttgacaaa
     7021 tttctttctt tttatataat agagtaacag acgcgaagaa aacgtttgcg tagtttgttt
     7081 taaaaaggag agaaaagtat ggctaaaaaa acaatcatgc ttgtatgttc ggctggtatg
     7141 agcacaagtt tactagtaac aaaaatgcaa aaagctgctg aagcaaaagg aatcgactct
     7201 gatattttcg ctgtgtccgc ttctgacgca gataacaatc ttgcaaataa agacgtggat
     7261 gtgttgttat taggcccaca agttcgcttt atgaaagctg attttgagaa acgtctggaa
     7321 ccaaaaggca ttcctttaga tgtcatcaat atggctgatt atggtatgat gaatggcgaa
     7381 aaagtgttag atcaagcaat cacgctcata ggtgaataaa tagaacagta taactatcga
     7441 acaagcgatc caaaaaatag atcgcttgtt ttttcgtata tttttttagg gcgaataata
     7501 tgaatcattt ttggggtaga aggcttctga cgagagatat taaagcaata gactgtgagg
     7561 aggtaaattt accaaattat gagaagatcg cagcagtgga acgtttgagc aactggattc
     7621 tttgtcatcg ctgtggaatg agaacgtccg tcattgatgg acagcttgca gattacggtt
     7681 atttttgtac acattgtcta tatttagggc gatgcgacac tcaacaagat ttatatttgt
     7741 ttgatcagcc tgaagcaaaa cctcgagaag tcgtcttttt ttggacagga cagcttactg
     7801 aaaaacaaac ggaaatctca aagagaatac tttgccattc agaaaaaaga catcatctta
     7861 tttgggctgt aacaggtgca gggaaaacag agatgcttta tcctatttta gtggaaactt
     7921 taaaagctgg aggaagagta gcgatttgta caccgcgagt cgatgtttgt aatgaattgt
     7981 ttttacgcta tcgttatgtt tttccagaag aaaaaatcac gttgctccat gggaatacag
     8041 caacggctta tacctttact tcttttgtta tctgtacgat tcatcagcta taccgttttt
     8101 atcggtcctt tgatttaata gtgatcgatg agatcgatgc gtttccttat aacggggatg
     8161 caggattagc tcatgctgtg caaacagcga ttaaaccaga cggacgattc atctacttaa
     8221 gtgccacacc tgataaaaag ttactaaagg agatcaagca gacgtttgac atccacaaac
     8281 ttgctttacg ctttcatcgg cgtttgcttc ctgaacccgt cttggttttc atgaataatt
     8341 ggcgacaaaa atgcaagaac aagaagaaga taaaatcttt ggtgcggatc attcatcaat
     8401 taattaaaga aaaccatatc cttttatttt gtccaagtat cacgatgatg catcgtttga
     8461 aagccacact tcaagaggct cttccgagat atgagatcac agatgtttct tcaagagaca
     8521 atcagcgggc ggaaaaagtc cagaaaatgc gagaagcaaa atatgacata ctgttaacca
     8581 cgatgatcct tgaacgtgga gtcacatttg aaagaatatc cgttatcgtt ttaggagcgg
     8641 atcatccggt ttttactaaa tcttctttgg tacaaatcgc aggaagagcc gatcgtaaag
     8701 ggccttatac gaacagtcaa gtatattttt tctatgaaga aaaaactgca gctattcgaa
     8761 aagcttgtca ggaaatcaaa gaaatgaatg aagaagggaa gaagatactg tgaggtgcag
     8821 ttgctgcagt aaagatatcg tccaaaattt aacattcagc gagattattg gtgtttcgaa
     8881 atacagatta cctcagactt gttcagtttg tcgcgcacaa tttcatttat tggaaacaag
     8941 aggctgttct ggctgtcaaa aaccaatcca tttcaatcta gagagtacga aaaagaaagt
     9001 aaattctatc cccgtttact gcagtgactg tcaagaatgg aagaagaaat atccgaccta
     9061 tgattttcag caccgtgcct tttttgtgta cgatgaaaat atgaaggcct ggctcaatca
     9121 atacaagttt atgggagata tccgactagc aggcaccttt gcatataaat ggacgcagat
     9181 aaaaaaagaa tacccgacat acatctattg tccaatacct ttatctgaaa aaaggcgaac
     9241 agaaagagga ttcaatcaag taagcgagat gttgaaagca gcaagaattc cttttcagat
     9301 gctactcaaa agagagaagc atctgctggc tcaagcaaaa aacacgcgaa aggaaagatt
     9361 ggagatgcct caaccttttg aacttgcagt caccttttct aagatacaaa ataaaaaaat
     9421 attgattatt gatgatgtct atacaacagg acagactctt ttccacgcag cagattgttt
     9481 gcagatcgca cagccgaaaa tcatccgaac tatttctttg gcgagatgaa ttagatgtgg
     9541 aaatttgata gcgatttaat ttgcaaataa cctagagttc gatataatag aattaagaag
     9601 agggaaagag tggtccttct ccttcttgat tgtggtagac gaaaggggta atttttatgt
     9661 ttagatataa tgtacgcggc gaaaatatcg aagtaactga agcgattcgc gactacgttg
     9721 agaaaaaagt aggcaaacta gaacgctatt tcagtgacgc acccgatgca acggcgcatg
     9781 tgaacttaaa agtttataca gaaaaaacag caaaagtgga agtaacgatc ccattacctt
     9841 atcttgtatt acgtgccgaa gaaacttctc cagatttata cgcaagtatc gatttggttg
     9901 ttgacaaatt ggaacgccaa atccgcaaat tcaaaacaaa aattaatcgt aaatcccgtg
     9961 aaacgggcat gaatactgca aatgctgctg ctttattcaa tgaagaggac gaaaacgaaa
    10021 atgattctga attagatatc gtacgtacga aacgcctttc attgaaacca atggatagtg
    10081 aagaagcagt cttgcaaatg aacatgctgg gacataactt ctttattttt gaagatgcag
    10141 aaacaaatgg tacaagtatc gtttaccgcc gtaaagatgg aaaatacgga ctgattgaaa
    10201 cagactgatc agccttactt aaataacaac gaaaacttgc agcagctact gacagctgcc
    10261 gcaagttttt ttgagtttcc tcctctttta gatgaaatat gaataaatta tgaagttctt
    10321 actttaatac gcttaagata tttcaattat ctataactaa tgataaaatg aaaagtaaag
    10381 tctacatagt ctggataata cacagaagga aaaggagatt acacaacgca tggccaattt
    10441 gattcgttca cttatcgaaa acgataagaa agaactaaaa cgtttagata gcattgcaaa
    10501 aaaagttgaa gcatacgctg accaaatggc agcattaact gatgaacaat tacaggcaaa
    10561 aactgctgaa ttcaagggca gataccaaaa aggagaaaca ttagatcaat tacttccgga
    10621 agcttttgca gtcgtccgag aagcagcaaa acgtgtatta ggtttatatc cataccacgt
    10681 acaattgatg ggtggaatcg tgcttcacga tggaaatatc cctgaaatgc gtactggtga
    10741 aggtaaaacc ttgaccgcaa cgatgccagt atatctaaat gctctgactg gcgaaggggt
    10801 acacgtagtt accgtcaatg aatatttagc aacccgtgac tcgacggaaa tgggcgaact
    10861 ttataatttc ttaggattaa cagtggggtt gaatatcaac tcaaaatctt cggatgaaaa
    10921 acgcgaggct tataattgtg atatcactta tagtacgaat aatgaattag gatttgacta
    10981 cttgagagac aatatggtcg tttaccgcca tcaaatggta caacgtccgc tgaattatgc
    11041 cattgtcgat gaagttgact caatcttgat tgatgaagcg agaacgccgc tgatcatttc
    11101 agggcaagca gaaaaatcca cagctttata tacaagagca gacaacttcg tgaaacgttt
    11161 gaaagaagaa gaagattata aaatcgatgt acaatcaaaa acaatcagtt taaccgaagc
    11221 agggatcgaa aaagcagaag aaacattcgg tttggataac ttatacgaca ttgaaaatac
    11281 tgcattgacc catcacttgg atcaagcatt gagagccaac tatatcatgc ttttagatat
    11341 cgattatgtt gtccaagacg gaaaagtact gattgttgac cagttcactg gtcggatcat
    11401 ggatggacgc cgttattcag atggacttca ccaagcaatc gaagctaaag aaggcgtaga
    11461 gatcgaagac gaaacgaaaa ccatggcgac gatcactttc cagaactatt tccgtatgta
    11521 caagaaactt gcgggaatga ctggtacagc taaaacagaa gaagaagaat tcagagaaat
    11581 ttataacatc caagtatacc agattccgac aaatcgtccg gttatccgtg atgatcgtcc
    11641 agatttgctt tacccaacat tacaaagcaa gttccacgct gtcgtgcaag atatcaaaga
    11701 tcgctaccgc aaaggtcaac ctgtgttagt cggaactgtt gcggttgaaa catctgagtt
    11761 attatcagat atgctgaatc aagaacgtat tcctcatgaa gtgctgaatg cgaaaaatca
    11821 ctttaaagaa gcagagatca tcatgaatgc tggtcaaaaa ggtgctgtga cgattgctac
    11881 caacatggct ggtcggggaa cagatatcaa gttaggactt ggggtaagag aacttggtgg
    11941 tctagcagtt atcgggacag aacgacatga atctcgtcgt atcgataacc agcttcgagg
    12001 acgtgcagga cgtcaaggag accctggtat gtcacagttc tacttgtcat tggaagatga
    12061 tttgatgaaa cgattcggtt cagaacgaat caaagctttc ttagatcgga tgaagatcgg
    12121 tgaggaagat gctgttatcc aaagcaagat gctgacgaaa caagtagaat ctgcacaaaa
    12181 acgtgtagaa ggaaataact atgacacacg taaaaacgtc ttacaatacg atgatgtgat
    12241 gcgtgaacaa cgtgaagtaa tctataaaca gcgacaagaa gtaatcatgg aagaaaaaag
    12301 cttatccaag ccactaatga acatggtcaa acggacaatc agtcgtgtag tagatgcgca
    12361 tacacaatta gagaaagaaa attggaattt ggaaagtatc atcgattttg ccggcagtac
    12421 tcttgtccat gaagatagta ttactcttgc agatatcgaa ggaaaaacag ctcaggagat
    12481 caaagagtat ctaaacgaac gagcacaaga agtatttaat accaaagcgg ctcaactaaa
    12541 tggtccagag caactgttgg aatttgaaaa agtcgtgatc ttgcgtgttg tggactctaa
    12601 atggacagac catattgacg caatggatca attacgtcag tcaatcggct tacgtgctta
    12661 tggacagaac aatccattag tcgaatatca aacagaaggc tataagatgt ttgaagatat
    12721 ggtaggcgcc atcgagtatg aagtcactcg aatctttatg aaagctgaga tccgtcaaaa
    12781 cgtacagcgt gaacaggttt ctcaaggaac agcttctcac tcagaagacg gagatgccaa
    12841 agaaaatagt aatactagcg cgaaaaaaca gccaatccat gtgaacaagg tcggaagaaa
    12901 tgatccttgt ccttgtggaa gcgggaaaaa atataagaac tgtcatggaa aaaatgcaaa
    12961 ctaatttctg acagcaggat tggtgagaca aattcttttt ttaagaattc gtctcacctt
    13021 ctttctgtta tttgctgatt ctgttagact agatataatg aaaaaagtat agtgaaagaa
    13081 ggaaaaaaca tggaaatatc cgaaattcga aatctcctga cagaaatgca ggaaaaggtc
    13141 acaagtttca ggaggtctct ttgacttaga tcagctggaa gaagacatcg cggaagcaga
    13201 agcaaaaatg gctgaacctg gtttttggga tgacagcgaa gccgcacaaa aagtgatcag
    13261 tgaaaataac gctaataaag aaacctatga ccaattccat tctcttgccg aagaactaga
    13321 agaaatcgaa ttgatggtgg agatgctgca agaagaacct gatgcagcaa tgcaagaaga
    13381 agcagaaaca cgtatcaatg cattagatga aaaaatgaag acgtatgaat tatctatgct
    13441 gttagacgga ccttatgatc gaaacaatgc gatcgtcgaa ttgcatccag gggctggcgg
    13501 aacggagtcc caagactggg gcagcatgct tttacgtatg tatacacgtt gggcagagca
    13561 gcatggctat caagtagaaa cactagatta tcaagcaggt gatgaagcag ggatcaagag
    13621 tgtgaccctg ctgattaaag gacataacgc ttttggttat ctaaaatcag aaaaaggtgt
    13681 tcatcgcctt gtacggattt cgccttttga ctcagcgaaa cgacgtcata cttctttttg
    13741 ttcggtagat gtgatgccag agcttgatga aacgatcgat atagaaatca atccagatga
    13801 tttgaaaatc gatacgtacc gagcaagtgg tgcaggtggt cagcacatca ataaaacaga
    13861 atctgctgta cggatcactc acttgcctac aggaacagta gtagctagtc aggcacaacg
    13921 ctcacaattg aaaaaccgtg agcaagcgat ggggatgtta aaagctaagc tttatcaatt
    13981 agaagtggaa aagaaagaac aagaagcagc tgcacttcga ggagaacaaa aagaaatcgg
    14041 ctggggttca cagatccgtt cttatgtttt ccatccctat tctatggtaa aagaccatcg
    14101 gacaaacgta gagacaggaa atgtccaagc agttatggac ggtgaccttg atttatttat
    14161 tgacggctat ttaaaaatgc atctacaata agaacccatt gttaaattga aataaaattg
    14221 taagattttg caagcatgtt gaaacattgc catgctataa tagctaaaga ctgaaaaaga
    14281 tatggagaga acgccatgat tgaaatgaag gatgtaatga agaagtactc aaatggtacg
    14341 accgctatcc gcaatctctc gattgtcatc gaacaaggag agtttgtcta tgtggttggt
    14401 ccgtctggag ccggtaaatc tacattcatc aaattgatgt atcgagaaga aaaagcttca
    14461 aaaggaactt tgaacgtcgc aggtcatgat ctaatagcaa tcaaaaaccg cgaagttcct
    14521 tatctacgca gagaaatcgg cgtggtgttc caagactata aattattacc aagaaaaaca
    14581 gtatatgaaa atgttgcgta cgctatgcaa gttatcggca gaaaacctcg agatatcaaa
    14641 aaacgagtaa tggaagtgct ggaccttgtt ggtttgaaac acaaagttcg agtctttcct
    14701 agcgaattat ccggtgggga acagcaacgt gtctctattg ctcgggcaat cgtgaatacg
    14761 cctaaagtac ttatcgcgga tgaaccgaca ggaaatctgg atccagaaaa ctcatgggaa
    14821 atcatgaaat tattagatcg gatcaatgcc caaggaacta ctgtcgtgat ggctacacat
    14881 aacagtacga tcgtaaatac gattcggcac cgagtaattg ctatcgaaaa cggccgcatt
    14941 attcgagatc aagcggaagg ggaatatgga tacgatgatt agaacctttt tccgccattt
    15001 attagaaagt atcaagagtt tgaaacgtaa cggctggatg acagttgctt cagtcagtgc
    15061 ggtaacgatc acgttgactt tagtcggtgt attcatggca gttatcatga atgcaactaa
    15121 attagctcag gatatagaag gaaacgttga tgtatctgta ttcgtagata tcggtacaaa
    15181 agacgaagag atgaaaaagc tagaaaaaga attgaatgac ttagatcatg ttaaaaaagt
    15241 tgaattttca agtaaagaac aggaattgaa aaaaatccaa aacgaaatgg gtgattcctg
    15301 gaaattgttt gaaggtgaca ataacccgtt atatgatgtg tatgtagtaa gtgctactga
    15361 tccttcttat acaaaacaag tagcagaaga agcagcagat ttgaagaatg tttctcgttc
    15421 tgattacggt ggagcttcct cagatcggat tttccaaatc gctggtgcag ttcggacatg
    15481 gggattagtt gcagcggcat tgctgctgtt cgtagcgatg tttctcattt cgaacacgat
    15541 tcggatcacg attttgtcta gacagcgtga aattcaaatc atgcgtttag tcggtgcaaa
    15601 aaatggatat attcgctggc cgtttttcct tgaaggtggc tggatcggtc tgatcggtgc
    15661 gattcttccg attttgatca tcacatttgg ttatgcatgg tttttccaat tgatcaatcc
    15721 atcattatta cgttcccatt attcattgat tcatccacaa aatatcgttt ggaaaatcaa
    15781 tcttttaatg gtcggcattg gtgtgatcat tggttcactt ggctcgatca tttctatgag
    15841 acgtttcttg aaattctaat accacaatat aaataaaatg agaaagttgg acagtttcgt
    15901 tccagctttc tttttttatt ggctaggaga atagaaggaa gcggcttttt tccctttact
    15961 ttaaaaaaca acttgggtat tcttaagttg gtagaggaag aggtgaagcc gtgaaaatta
    16021 tcgttgacaa tttttttacg atccttttga ttatgaatat tttgctttcc tttatcatcg
    16081 tttttcgaga aagaaaggaa actgctcaaa catgggcatg gctgctcgtt ttgatgttca
    16141 ttccagttgt aggatttatt ttatatattt ttttaggaag agggatttcc aaagagaaaa
    16201 tctttgattt gaagatccaa gggaaaatcg gtaagaacct ggaaattgaa gaagacaaac
    16261 aggcattgat gcgagggctt tatcctcatc cgcctactgg acaagtagat gtgaaacaat
    16321 tgatctatat gctcacggta tttgaaagta cgctctatac gactaaaaat gaaatcactt
    16381 tatttacaga tggtcgtgaa aaattcgatg cgctgattga agatatcaag caagcagaag
    16441 accatatcca cttccagtat tatatttatc gcagtgatgc attaggtgaa gaagttcgag
    16501 atgcattgac agaggcggca agacgagggg taaaagtcag agtccttctt gatgcatggg
    16561 gttccacaca agtttcttcc agcttttttc agaatttgaa aaaagcaggc ggagaaatcg
    16621 catttttctt tcctctattt gttccgtata ttaatcctcg gatcaattat cgcaatcatc
    16681 gtaaaatcgt agttattgat gggaaaatcg gatatacagg tgggttcaat gttggaaatg
    16741 aatatctagg attggtaaag aaatttggtt attggcgaga caatcatttg cgtatttacg
    16801 gagaagcggt gtatatcttg caaaaccgtt ttttgatgga ctggaattct cagcatacaa
    16861 aagaagtagg ctattctccc aaattttttc cgtccattca ctctactggt gatatagctg
    16921 ttcaaatcgt gacaagcgga ccagataccg aacatgaaca aatcaaaatg acttatttga
    16981 aaatgatttc gttagccaaa cgggagatat tgatccaaac gccttattac ataccggatg
    17041 gatcgattca tgaagcactg aagttggctc ttctttcagg cgtaaaagtt catatccaaa
    17101 tacctaacaa accagatcat cttctcgttt actgggcaac gtattctttc gctgcagagc
    17161 tgatcgagta tggagcaagg atcgaaacat atgagaatgg atttattcat gcaaagacga
    17221 tgattatcga tggtggcatt gtttcagttg gctcagcaaa tatcgatgtt cgttcatttc
    17281 gcttggactt tgaggtcaat acactgattt atgatgaacg aatggcttca cgtgtaagaa
    17341 aagcattttt tgaagactcg aaaatttcta cgcatctcac aaaagaaatg tatgaaaacc
    17401 gaggaataat catcaagatg aaagaaggat tagcaagatt gatttcgccg ctcctttaaa
    17461 acacaaaaag gacatgagaa ccatcaacag atggagatct cgtgtccttt tctaacgggg
    17521 ccatttctga gagtacgtca gaaatggaga aagcgtctcg catgagcgaa attaactttg
    17581 gggtatctct tgttttttgg ggaaagcaag aaataagcta atatatgcta ttaaaagaga
    17641 cacatttcaa agccagtata gcatagaaac ttttaatgag atagtttttt ttacaattat
    17701 gaggatttat tttgttattt aatgattttg attcgttttc atcaagtaat gaaaatgata
    17761 aaataaacat actttaggat taaaggagat ttctaatgaa aaaacttaaa ttattcttac
    17821 caataatagg attagctatt cttttatcag gctgttcaaa gtggattgat cgaggagaat
    17881 ccattacggc tgttggctct tctgctttac agcctttagt agaaacagtt gctgaaactt
    17941 accagacaga aaatcctgga aaattcatca atgtacaagg gggcggaagc ggaactggtt
    18001 tgtctcaagt acagtcagga gcagtcgaca tcggaaactc tgatttgttt gcggaagaaa
    18061 aatcggggat cgatgcctca gcattagtcg atcatcgagt agcagttgtt gggatcactc
    18121 ctattgtgaa caaggatatt ggcgtaaaag acatcagtat ggaaaacctg aaaaaactat
    18181 ttacaggcga gatcaaaaac tggagtgaag tcggtggaaa agatcagcca gtagttatat
    18241 tgaaccgtgc ttcaggaagc ggaacccgta gcacgtttga aaaatgggta ttggacggag
    18301 aaacagctgt tcaggcacaa gaacaagatt caagcggaat ggtgcgccag atcgttgccg
    18361 acacacccgg tgcaatcagc tatgttgctt tttcttatgt ttccgatgat gtagatacgt
    18421 tgaatgttaa cggtgtagaa ccgacagatg aaaatgtgac aacgaacaaa tggacgattt
    18481 gggcatatga gcatatgtac acaagagatc atccaaaaaa actgacaaaa gaatttttag
    18541 actatatctt atctgatgaa gtacaagata acatcgttgg agaactaggt tatatccctg
    18601 tttctcatat gaaagtcgaa cgtgattggc aaggaaatat cgttaaagag taatctttac
    18661 acaaaattta caaacttagc taaactttta catgttatta aaaccaaatt gatgttcaat
    18721 ttgtacactg agtgagtatg ttaaacctga ttaaggggga gtcatctgtg gaagatgtga
    18781 aaaaagtatt attaacaaaa tcaaaacgtt caaaaatgga acagcgtgga aaattcatca
    18841 gttttttatg tatcgcgctg atcgtgttgg ttgtattatc tattttttat tttgtagcta
    18901 gtaaaggatt ggccaccttt tttactgata agatcaatgt atttgatttt ctgtttggta
    18961 cagattggaa tccaagccaa atcggagcaa atgggaaacc aatggtaggg gcattgccta
    19021 tgatcacagg ttcgtttatt gtgacctttt tatctgctat tgttgccaca ccatttgcca
    19081 ttggtgcagc agtattcatg acggagatct ctcctaaaaa cggaacgaag atcttacagc
    19141 cggtcatcga gctgcttgta ggtattcctt ctgtcgttta tggttttatc ggtttatcag
    19201 ttatcgtgcc ttttgtcaga agtatttttg gcgggacagg gtttggtatc ttagctggta
    19261 catttgtgtt gttcgttatg atcttgccga ctgtcacaac gatgactgtt gatgcattaa
    19321 aggcagtacc acggcactat cgggaagctt cattagcgct tggtgccaca agatggcaaa
    19381 caatctacaa agtcgtgcta cgagcagccg tgcccgggat tttaacagca gtcgtttttg
    19441 gtatggctcg tgcgtttggt gaagccttag ctatccaaat ggtcatcggt aatgcagcat
    19501 tgatgccaac aggacttact actcctgctt ctacattgac gtccatctta acaatgggaa
    19561 tcggaaatac aatcatggga acagttgaaa ataatgtgtt atggtcatta gcgctgatct
    19621 tgctattgat gtcattattc tttaatatcg tgatccgtat gattgggaag aaaggagagc
    19681 tgaaataatg aacgcaaaac gctcagataa aattgctaca ggtgttttat atgtagtttc
    19741 ggggatcatc gtattgattt tagcagcttt actactttat atcttagttc ggggaatacc
    19801 gcacatttca tggtccttct tgaccgaacc ttccaaagcc taccaagaag gcggtggaat
    19861 cgggatccag ctatttaact cgatttattt gcttttgatc acgatgatca tcagttttcc
    19921 tatttcttta ggtgcgggta tctatctttc agaatatgct ggaaaaaact ggctgacaga
    19981 tgtgattcgc acgtcgattg aaatcttgag ctcattgcct tctgtcgttg tcggtttatt
    20041 cggtttcttg gttttcgtta ttcaattcca gtatggtttc tcgattattt ctggtgcatt
    20101 ggctttgact gtattcaact tgcctctttt gactagaaca gttgaggagt ctttacaagc
    20161 ggtccattac acacaacgag aagcaggatt ggctttaggt ttgtcacgct gggaaacagt
    20221 gataaaagtg gtggtttcag aagcgacacc agggattttg actggtgtga tcctaagttc
    20281 tggaagaatt tttggtgaag cagctgcatt gatctatacg gctggacaaa gtgcacctgc
    20341 gttagatttt acgaactgga atccattaag tgtttcaagc ccgatcagca ttttccgtca
    20401 agctgaaaca ttagctgtcc atatttggaa gatcaataca gaaggcacga tgcctgatgg
    20461 tgtttctgtt tcggctggcg cttctgccgt gttgatcatt gctgttttac tgttcaactt
    20521 cggcgctcgt gcgattggca aacgattata ccgaaaaatg acttcagcct aaggagaaga
    20581 agcaaaatga aaaaatataa ctggaatgaa acaaatgtca tcaccatgcc aaaagagaaa
    20641 gaagtagcac tatatacgga tgatctacat gtctggtatg gcgacaatga agcaatcaaa
    20701 ggagtggatc tggaatttga aaaaaataag atcactgcac tgattggtcc ttccggctgc
    20761 ggaaaatcta cgtatcttcg ttcgttgaat cgaatgaatg atgggatcgc taatacgaaa
    20821 atcactggga aaatcatgta tcaaggcgta gatattaatg cgcctcaagt ggatgtttat
    20881 gagatgcgca aacggattgg catggttttt caacgtccta atccgttcag caagtcgatt
    20941 tatgacaata ttacatttgc tttgaaacag cacggcgaaa aaaacaaaaa gaaattagat
    21001 gaaatcgttg aaacaagctt gaaacaagct gccttgtggg accaagtaaa agacagcttg
    21061 gacaaaagtg ccttggcttt atctggcggg caacagcaac gtttatgtat tgctcgtgcg
    21121 atcgcgatga aacctgatat cttgctgcta gacgagccgg caagtgcttt ggaccctatt
    21181 tcgacaggaa cggttgaaga aacacttgta aacttgaaag aagactatac gatcatcatc
    21241 gtgacgcata acatgcaaca agcggctcgt atcagcgact atactgcctt tttctatcta
    21301 ggaaaagtaa tggaatatga caaaactaac aagatattta ccagaccaaa aattcaggca
    21361 accgaagatt atgtgtccgg tcattttggt taagacggag gcctcaacat gacaaaagaa
    21421 attatttcat ctaaagatct tcatctgtat tatggaaaga aagaagcact aaaagggatc
    21481 gatctaagtt tcaatcaagg ggaaatcact gcgatgatcg gtccttctgg ttgcgggaaa
    21541 tccacttatc tgcgttcttt gaaccggatg aatgacctga ttccgaatgt aacgatcacc
    21601 gggagtgttt tatataaaga caaagacatc tatggtccaa aaacagatgt tgttgaacta
    21661 agaaaagaaa tcggcatggt attccagcag ccaaacccat ttcctttttc tatctatgaa
    21721 aatgtcatct atggattgaa attaaaaggg gaaaaaaata aaaaagtcct tgatcaagta
    21781 gttgaagaaa gcctgaaggc agcgtctgtt tgggatgatg tgaaagacaa attgcataaa
    21841 agtgctttat ctttatccgg tgggcagcag cagcgtgtat gtatcgcacg tgttttggca
    21901 gttaatccgg aaatcatctt attggatgaa ccaacaagtg cgcttgatcc tgtttctact
    21961 gggaaaatcg aaagtatgct gttagaatta agagagcggt atacgatgat catcgtgacc
    22021 cataatatgt ctcaagcttc acgtatttca gataagacgg cattcttttt agatggtcac
    22081 ttgatcgaat acaatgatac gaagaagata ttcatgaatc cagaaaaaca agagacagaa
    22141 gattatattt caggtcgatt tggataagga ggaaacggaa tgctacgtac acaatttgaa
    22201 gaagaattgc tgaatctcca taatcagttc tacgaaatgg ggatgatggt gagcagcgcc
    22261 gtgcacaagt cagttcgtgc atacatcaaa cacgataaaa tcttagccca agaagttatc
    22321 gaaaacgata taaatatcaa taacatggaa acacgcttgg agaagaaaag ttttgaaatg
    22381 atcgcattgc aacagccagt tacaactgat ttgcgtatga tcattacggt catgaaagct
    22441 agctcggatt tagaacgtat ggccgatcat gcggtgtctg ttgctaaatc cacgattcgc
    22501 gtaaaagggc agacgagaat accggaaatc gaaaaagaga tctccgatat gtccgattat
    22561 gtgaaaaaaa tggtggacaa cgtcctagtt gcttatgtca aaacagacga aaaagacgca
    22621 cgtacgatcg ccaatatgga tcaaagagtc aatgactatt tcaatcgaat ctataatgca
    22681 acaatcaaaa atatgcagga aaatcctgag acagtcatca gtggaacaga ctacttgaat
    22741 gttgccagtt atctagaacg gatcggagac tatgtgacaa acatctgtga atggatcgtc
    22801 tacttggcta cagggaaaat cactgagtta aatacgaacc acaatgagat tttctaaact
    22861 aaagcggtga ttctgccgtt tgtttaggaa aagaggttgt gaaatcagcg gttgtgaaaa
    22921 agctgttctg catcaagtaa taagaacagt caaacaaaaa tagctcctca tatttttcat
    22981 ttggctggac ttattaccgc agatgcaagc ttttgaacac tgtttattcg ggaaagggtt
    23041 tgtgacaaaa tatgtcacaa acccttttca tcatttcata gaaaggataa agaatgcaaa
    23101 aaaaaattag catcatcact atatttttta tttgtgtgct tacttcatgg gttattcagc
    23161 atcaagctgc tttatatgaa gtacctgtca tcgagatcga aaaagtacga ataaaagagg
    23221 atcatcaatt gattacagga agactgatga acacgaatca aaaaggagaa tcgatttatt
    23281 tatcggttcc ttatcaaaaa aatcaaatag aaacagcaaa tataaaagcg agacaaatgt
    23341 atctagtcca aatgcatgga gatgaatggc agctagtagc aaaaaaaaat gatggctggc
    23401 ttttcttctt cacaagtatc ttcattggcg ccatgttgct tattggcgga aaacatggtc
    23461 tatttgcttt aggaagtctc atcatcaata tcgttttact tttacttttg ttgctcatct
    23521 ttactaaaat agaagggatt tctctattaa atatatctat cctgttcgtc gttataggga
    23581 tgattattag catattggct ttagatggat ggaatagaag cagtataatc aaaatttcag
    23641 ctgcagttac gagtgttttt ttagcatttt ttatttgtct tttgacaatg aatctttgga
    23701 aagaccaagg actgcgctat gaggaactga attatctgac acgaccgtat cgttctgttt
    23761 ttctttctag cgtactgatt ggaacagctg gaggaacact cgataccgtt atcacagtca
    23821 ttgcgacatt agaagaattg actaagcgac aaccagaaat aacagcgaaa cagttatggc
    23881 tttcgggaag gcaagtgggt cgagatgtga ttggctcgat gaccaatatc cttttgttcg
    23941 tctatatcag tgggtcagtt ccatcgcttc ttttatattt aggaaatcaa tggtcgttca
    24001 aagaaacgat agaacagcat ttatcattgg aatttttacg cgtcattgct ggcagcttag
    24061 gaatagtatt atcgatccct atctcggtcc tcttttttct gactgtaagg aggctgaaac
    24121 gatgaatgta cttattatac ttgttgggat tttagcaatc agcgtgctac ttattggcgg
    24181 tacgcaaggt atgtatattc ttttaggctt attcatcaat ttagggatct ttttcttgtt
    24241 gttattcggt tatcatcaga agtggccgat actggcattg tcggtcatag gatttttatt
    24301 gattgctgtg gttatcttat tttttatcaa cggttataat ctaaaaatgc gtgcagcttt
    24361 tgtgagtata ctgatctttt tattttgttt cctactattg cttcctatca ctgatttttt
    24421 agcaatccaa ggttttacaa cgatagaact agaagaatta tctggcttag ataagacttt
    24481 agcgattgat tttagattac ttacgagatc gttgcttctg attagtctta gtggtgctgt
    24541 tttagatgcg agtgtagcaa tttctagtgg gacattcgaa gtatatcagg caaatccgca
    24601 attatctttt taccaattgc gccattctag tttcgctatt gctaaaaaaa tattggcgtc
    24661 aacagtaatg acacttcttt ttgcattcat gggaagttct ttagcgttga tcctctggtt
    24721 tattgattta gacattcctt ttcagcaagt gatcaatgaa aaatcatttg ttctagaata
    24781 tacgatggct attcttacca cattgtccgc actacttgtt cttcctttaa cctgcgtgac
    24841 tacagcatat ctctttacaa aaaagaaaga acaactcaag atggagtaaa agttacaact
    24901 taagtcctat gacattttta ctgaatcagt gcataatatg gatgtaagga aaagcttgca
    24961 aacaattttg cttaaggagg caatttccat gaaagaaaga gaacgtattt tagatttagt
    25021 gaaaaaagga atcctttcga ctgaagaagg cttagattta ttagaaagca tggcaactga
    25081 aaaagacgaa aagcaaatca aaaaagaagc agacaaggta actgctagtc ataaagaaaa
    25141 agatcaggca agtcaattga tcgataaact tgaaaacggc gaagaagaaa tttctgagcc
    25201 agttgatcca aaggaaagag aaagacaaga tcaagaaaac ttagaaaaga tcttagatga
    25261 actagcaact gaagcgaata aaacttcggc gcgtctagat gaagtaaatg ctcaattcgc
    25321 tgacaataag gcagcacgca atgagaaaca ggaagcattg atgcagctga atacaaaaga
    25381 agaattaggc gagttgacag aagaagaatt agcacagcgt cagacgttag aagcagaaat
    25441 caaagagtta gaagctagtg gcgatacact gttagaagaa caattgaaat tagaagcaga
    25501 attaaaagat atccgtaaga atcaatggtc tgagaaaaaa gaaacgttta ctgataagtt
    25561 tgaattgcca gatgattgga aagatcaagc aacagataca ctgaatcaag taggcgaaaa
    25621 aatgtcagaa gctggatcac agttaggaaa attcttgaag aaaacattcc aaactgtttc
    25681 agaaacagtg aatgacaaca tggaatggaa agatgtcagc ttgcgtgttc ctgggattgc
    25741 gacaacgaaa tttgagcatg aattttacta tgaagctcct gcagcaagta tccttgatat
    25801 caaggcagca aacggaaacg tgacgttaaa aacgtgggac agcaacgatg tgaaagttga
    25861 agctaagatc aaactttacg gaaaaatggg tgctgaacca tttgaagcat tctctgaacg
    25921 gagccagatt gaggttaatg aagatcatat ctccttccaa attccaaata aacgtgtgcg
    25981 tgcagacctt gttttctact tacctaaacg agtatacgac cacgcagcta ttaaacttct
    26041 aaatggcaac atcatgattg aaacattgga agctaaagat atttatacaa aatctacgaa
    26101 tggtaacatc atcgtggacc aattaactgc aacgatgctg gaagtagaag gtgtaaacgg
    26161 caatatcgat attcgaaacg ggaacatctt agattctatt atcgaaacag taaatggtac
    26221 agtgacattt ggtgcgacag cagaaaacct ttctgtgtct ttggtaaatg gtgatgtacg
    26281 tcttacgatt aaagaagata atttgaaaaa agtagaagca agttcagtga acggaaatgt
    26341 caaagtcgct ttaccagatg gcatcggttt ggaaggacat gctaaaacaa gtcttggaag
    26401 tatcaacagc cgtctgtcca attacgaagt agttcgtgag aaaaaagaac ggacaaacca
    26461 aatgttgcaa ttccgtcgtg tttcagatgg tgaaatcgcg caagtgcaac tttcaacgac
    26521 aacaggaagt atctatttaa aagatacaga taaataaaga ggttgtgaaa gagatgttca
    26581 gccttgagca ataagaagcg attttgtaaa atagcttccc ctattttaca aaattgtgcc
    26641 ttattgccga taggctatct cttgaacacc gtttaccaag agattatgaa cttgccaatt
    26701 atcgccgagt cacaaggaat aatttcaaaa aaagctcgtc acgttttatg aatatggtaa
    26761 tttgtgaccg agaattttgc aaaaagacac cgattaagaa gaaggttgtc acaacgattt
    26821 tcttcattta aaatagtaga acagaggtga gacatgatga aaaaaaagtt gacaaaatcc
    26881 aataaaaatg ttgttttgac agggacttta gcaggaatag gagaatacat cgggattgat
    26941 ccgactgtta tccgtgtgct ttatgtattc ctgagcttag ttgcttttgg aagtccaatc
    27001 attttataca ttttattagc tttgattatc ccatctgcgc gttctgcacg aacaaactat
    27061 ggacataaca atgcatatta tgaaaaaaac aattatcgtg aacaaactcg tgaacaaaca
    27121 aaaaaacgca aagaagcgga aaaaatcgat gatgacgaat ggagtgactt ttaatgtcgt
    27181 actttcaacg tattttagtt aatacgttaa cattcatatc attggctgtt ctttttcctg
    27241 cgatggtcca tgtgagcagt atttggacgg ccgttcttgc cagcttcgta ttgtctgtgt
    27301 tgaatatgtt tgtcaaaccg attttgacga tactgtccct gccctttaca ttagtcacat
    27361 tgggattgtt tacgtttgtt atcaatgcag cgattcttaa aatgacctcc ttttttgtgg
    27421 gagcagaaca ttttagtttt tcttcattcg gtgcagcgtt gttagtagct atcttgatgt
    27481 cgcttgtcaa tgtaattgtc agcgaacata acagtagaaa ataaaagaaa gctgtgccga
    27541 actatcggcg cagttttttt
//
LOCUS       DACDRN010000031        27485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDRN010000031 DACDRN010000000
VERSION     DACDRN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.031"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWJ80_001574"
     CDS             6..305
                     /locus_tag="IWJ80_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9461291.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWJ80_001575"
     CDS             378..1739
                     /locus_tag="IWJ80_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP9461292.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWJ80_001576"
     CDS             1761..2021
                     /locus_tag="IWJ80_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461293.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWJ80_001577"
     CDS             2035..2787
                     /locus_tag="IWJ80_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP9461294.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2950..4290)
                     /locus_tag="IWJ80_001578"
     CDS             complement(2950..4290)
                     /locus_tag="IWJ80_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9461295.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(4423..5028)
                     /locus_tag="IWJ80_001579"
     CDS             complement(4423..5028)
                     /locus_tag="IWJ80_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9461296.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(5081..6055)
                     /locus_tag="IWJ80_001580"
     CDS             complement(5081..6055)
                     /locus_tag="IWJ80_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP9461297.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(6433..7212)
                     /locus_tag="IWJ80_001581"
     CDS             complement(6433..7212)
                     /locus_tag="IWJ80_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9461298.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(7279..8550)
                     /gene="celB"
                     /locus_tag="IWJ80_001582"
     CDS             complement(7279..8550)
                     /gene="celB"
                     /locus_tag="IWJ80_001582"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9461299.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            8703..10100
                     /locus_tag="IWJ80_001583"
     CDS             8703..10100
                     /locus_tag="IWJ80_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9461300.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDFFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            10181..10621
                     /locus_tag="IWJ80_001584"
     CDS             10181..10621
                     /locus_tag="IWJ80_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9461301.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            10769..11095
                     /locus_tag="IWJ80_001585"
     CDS             10769..11095
                     /locus_tag="IWJ80_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9461302.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            11096..11845
                     /locus_tag="IWJ80_001586"
     CDS             11096..11845
                     /locus_tag="IWJ80_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461303.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(11925..12380)
                     /locus_tag="IWJ80_001587"
     CDS             complement(11925..12380)
                     /locus_tag="IWJ80_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9461304.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(12807..13082)
                     /locus_tag="IWJ80_001588"
     CDS             complement(12807..13082)
                     /locus_tag="IWJ80_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461305.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            13376..14191
                     /gene="thiD"
                     /locus_tag="IWJ80_001589"
     CDS             13376..14191
                     /gene="thiD"
                     /locus_tag="IWJ80_001589"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP9461306.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(14520..15971)
                     /gene="cls"
                     /locus_tag="IWJ80_001590"
     CDS             complement(14520..15971)
                     /gene="cls"
                     /locus_tag="IWJ80_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9461307.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            16312..18978
                     /locus_tag="IWJ80_001591"
     CDS             16312..18978
                     /locus_tag="IWJ80_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9461308.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            19342..20094
                     /locus_tag="IWJ80_001592"
     CDS             19342..20094
                     /locus_tag="IWJ80_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9461309.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(20116..21270)
                     /locus_tag="IWJ80_001593"
     CDS             complement(20116..21270)
                     /locus_tag="IWJ80_001593"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP9461310.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            21455..23896
                     /locus_tag="IWJ80_001594"
     CDS             21455..23896
                     /locus_tag="IWJ80_001594"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP9461311.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(24226..24930)
                     /locus_tag="IWJ80_001595"
     CDS             complement(24226..24930)
                     /locus_tag="IWJ80_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461312.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(25117..25362)
                     /locus_tag="IWJ80_001596"
     CDS             complement(25117..25362)
                     /locus_tag="IWJ80_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461313.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(25379..26230)
                     /locus_tag="IWJ80_001597"
     CDS             complement(25379..26230)
                     /locus_tag="IWJ80_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9461314.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            26396..27400
                     /locus_tag="IWJ80_001598"
     CDS             26396..27400
                     /locus_tag="IWJ80_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP9461315.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         8709 a         4833 c         5695 g         8248 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttaggg ctcttcgtca atgagtgttg gtgaagaaat tcaatagaat aatcgagata
     2941 ctcacacggc tagacagcta tcttgtctag ccgtgttgtt gttctggctt tagtcagttg
     3001 ggtagtgttc ttagttttct tgaaatattt tcctcgatat aaaaagattc gttctcggta
     3061 gttttgaaag tttctaaagc cataggcaat tcgttttagt actttgatat ggttgttcaa
     3121 acattctata gggccgttgg aatagggaac tcgcaacgct cgtttgattt ctgttcgata
     3181 tttcttgaag gtcgtgaaca ctggttggta gtgctctggc aactccttgt agtcttcttt
     3241 taggagcgcg acgaattcag gaacatcttg tcttctgata gcggaaagaa aaacctggta
     3301 gacttcgtag cctcgcttta aggagtcatc ataatataat agccgatcga ctatctccgc
     3361 ttctgtcaga tgatctcgaa atgacggacg ccagtagcga tgctgaccat tcaattccca
     3421 cgcattcttt tgaagtagtc tccagtattt ctttaagtgc ttcgaccgac gatccccttt
     3481 tttaaaggtc ttcattactt gaattcggtg gttggtaaag gcccgaccga tgtgttggct
     3541 aatatgaaag cgatcggcga cgattcgggc attcggaaaa cactctttca ccaatgtgcg
     3601 atagggtgta tagaaatctg aaaccactag acgtacttgt tcacggactt ttctagggta
     3661 gcgcaagaaa tagtcgcgta aaggattgag ttgccgattt tccacaatat caatcagttg
     3721 atgggtcttg ccgtccatca tgacaaagct catggctccc gagacgtttt tcaccgattt
     3781 gaattcatcg aaacacacca cttctggtaa cggctggttc aaagaaacgg ttttaggccg
     3841 aaggctgcga agtattcgaa tgacagtggt cggagaaatg tgcttcatac gtgcaatcaa
     3901 agacatagaa ataggctcac ttaatagctc aagaatggct tgtttgactc tgcgggcaat
     3961 ggagcagcga cgatcgctaa ctgaatcttc tgctaaaaaa gtctttccac agtgtttaca
     4021 tttgaagcgc tgtttgttaa tccgcacaat ggtcttgtag ttactgacat cattcaataa
     4081 gatggatact tttttccagc cccatttaat gatcgtttgc ttattttgat agtggcaatg
     4141 gaagcattcc tctggttgat aggtcaatgt gccatcaaaa acaaaacatg tttcgtcatt
     4201 aatgcgctct ttgcgaaagg catcttcatg aaaggtaaga tttaggtcta gaatatctag
     4261 taactctttg gtaaactctg aataggacat cttgaaacac tcctttgaat ggttttcgtc
     4321 gactttattc taaaggattt caaggtgtct gtttgcgttt attagaaaaa agaaaaagta
     4381 ttggcaacag aaggcgtcac caacactaga tattatagat ccttatttag ttaagaggtg
     4441 gtctgtccat tccctcgctt gcttgatttc gtccatcgtc aactgatgac ctgttacagt
     4501 tagctgaata gtcacatttg cttggcgtgt ttcgaattga tcggccaatt ctttaaagga
     4561 agcaggggaa acgattggat cgttatcacc ataagaaatc cagacggttt tatcctctaa
     4621 tgtggaacgg ctttgatgaa cacctagaga catcggatgg aaaaacaacc ctttatttag
     4681 ctcggtctca cgctccaata ataaatgagc agcgatgttt gcgccatttg aatagccgac
     4741 aagcagccag tcacttaacg gtatcagttc tttttcgctg atttctttga ttttttcatg
     4801 gagtttatct gtttcttttt ccaaactgtc caggtcgaat tgattcaatc cattacgctt
     4861 gaaaaaacga ttcatcccat cttcttggac cgtgccacga aaagaaagca atgtactgtt
     4921 tggtgcaaga aagtgagcga tatccagtaa agaatgttca tcgccacctg tgccatgaag
     4981 taagatcaaa tgcttgccac ttgttccttt ttcaaaaatg taggtcattt tgttccctct
     5041 tttctgtttc tatagatgca aatgaagtag catgaagaga ttattcacgt tttttatttg
     5101 catcacttgt atcgaaagga cggacataag cttcaatggc ttcacgacgt tcttctaaga
     5161 atggaggaag agatagtttt tctcctgctg attctgctgg ttcatcttca aggaatccag
     5221 gaccatcagt tgctaattca aacagtacgt gatgagcggc taggaagtac tcagattgga
     5281 aatagaaacg atcgacgaaa ccagaatttg gcaattcaat cttattgatt acttgaatcc
     5341 agtattccaa tgcttctttg tctgctacac gcaatgctaa gtgatggata tttccaaatc
     5401 cttcgattgc ttttggcaag tctgtccgat gttcaataat gatgcttgca ccatttcccc
     5461 cttcaccgat ttcaaataag tagaaatctc ctgaatggtc tgtttctttg aatcctaaga
     5521 ttttttcaag aatcaagcgg atatgctcta attgactgac acggacaaag acaggtccaa
     5581 gtcctgtgat cgcaaactct gtcggtacat tgctgttttt ccaaggaatg ccagctgcta
     5641 caccttgatt gttttcgtca gagatcaatt gataatactg attatcgaaa tcttcaaatt
     5701 cgatatattt tttgccgaaa cgttcattga ttgctgaatg agtaacgtca tatttttcaa
     5761 aacgttccaa ccaaaagtct aatgctgcgt cacttggcac acggaaagaa gcacgagaga
     5821 tcgtatctgt tccttttttt ccttttggct gatttggaaa atcaaagaaa gtcatatctg
     5881 ttcctggcgc accagtatcg tctgtaaagt aaagatggta agtttcgatg tcatcttggt
     5941 taactgtttt tttgattaag cgcattccta aaatatctgt aaaaaagcgg tagatttttg
     6001 tagcgctaga tgtcattgct gtgacgtggt gaattcctct gatttgtgta tccatgataa
     6061 atttcctcct gttaacttac aaaaagtaag tagttgattt agacatagta tagagggtgc
     6121 atctctattt tgcaattaat ttgtcttcta aagtatcagg aaggaatcaa gaatggaaaa
     6181 gaggagggac ggatcaataa aataaaaaga cttacgacta gctgtatcga tttcaaaaaa
     6241 actagcttta aaaagctaac tttagaatca ttacgggtgg tcataagtct ttttatgctg
     6301 agttattttt tcatttttga gctaatgctt aggatagaat agaatgttga tttcacgatc
     6361 tcttcttgtt acttgaaact gcttaccact gtcacgatct cttggtaaac ggctttttca
     6421 caacctcttt ggttaagttg aacgcacatc cagcacagaa tagttccgaa catcaaatcg
     6481 gtttcggctg gaagaatatt caatcggctg tccatctttg agatagacga cttgctgaac
     6541 ttccagtaca ggatcgtttt ctgcacagtt caaatattgc tgatcatatt catcactttt
     6601 atctgcagaa atagcacgat aagcccctgc aaaatggaca ttcaattctt gatgaagatg
     6661 atcatagata gaggctaaca aatgttcttt ttttaaaccc ggcacaagat ggactggcat
     6721 aaaggtatgc tctaaaacat atggctcgcc atctaagatc cttaaccggt gaatattgta
     6781 gacaggttgc tcggcatcga tcatcaatcg ttcttggata tattgattag ggaattcaac
     6841 atcaaaatga atgatttgac tcttcaaaga acgatgtctg cgtttcattt ggagactcaa
     6901 accttcatac tcgttagcgg gcgatgtgtc tttttgcata aatggatgat tcaagatttt
     6961 cgttcctgat cctcgctgcg aatagacaag tccttccatt gctaagatat tgatggcttt
     7021 tttgattgtc atccggctcg caccaaattc ttttaccaag tcgacttgat taggcaaaaa
     7081 actatcgaga ggatactctt ttgaacggat acgttctctt aaaatatttg cgatttcttc
     7141 gtattttggc acgttattca cctcttctag agtataacga aagattatgc ttccgacaac
     7201 tcgaagtagc atctggaaca ttgtttattc gaaaaaagag gtcatgacaa gtttttgtca
     7261 caacccctta atgatctatt aagcaatatc taaatctggt tgtttatcta gtactttcag
     7321 gaatgggtac cagataaagg caacgatgac aaagtcgatg acttgcagca taccaccaag
     7381 ccatgaattt gttgctaaga caccgcttgc aatgattggc acagtccatg gaacagagac
     7441 gccagttggt gcagggacaa gtccagcagc cattaccaaa taattgaacg tcgttacgat
     7501 tagaggtgcc acaacccaag gaataaggat tgttgcattc aatacgatcg gtagaccgaa
     7561 gatcgctggt tcatttacat tgaaaatacc aggtgctaaa gctaatcgtc caacatcacg
     7621 atactgtttc tttttaagaa caaaggccat caataaaacg acagctaaag tcattccaga
     7681 gccaccaata ccaacagtga aggtttccat gaatggtttt gtgatgatgt gtggtaattc
     7741 ttttccatgt tgataagctt ctaagttatc aagcatcagt gtattccaaa tcgggtccat
     7801 tacagagttg acgatgattt gtccgtgaag tccgaagaac cataagaatt ggacaaagaa
     7861 caaagcaatc aatgttgcag gtaaactact tcccaaacca gttaatggtt tttggatcac
     7921 ttcataaacg acgtcatgta agttagcatt gaaaatacca gtcatcacag cattgattac
     7981 taagaaaata gttagtgtca ctacagcagg gatcaaagcg gcaaatgatt ttgttactgc
     8041 aggcggaact ccatcaggca ttttgatctg ccagccgcgt ttcgtgattc tgcagtagat
     8101 ttctccagcc aagaaagcag cgatcattcc aatgaacatt ccttttgcgc caagacgatc
     8161 aagtgaaagc acaccagtca catcttcacc gccagcggtt ttcattgcaa aaggcgtaag
     8221 aatcaagaag ctggcaaaag acacggcacc accgaaaata ccttcaacgt cataagattt
     8281 tgataaataa tagccgatac caaaagtaac aaaaaccgac atgatgctca ttgttgcatt
     8341 ttgcccatta ccgaataaat tcgataaagt tccctttgtt gcgtcactga aaaaaggcaa
     8401 gttattgata acgacaacga tcgaaccgaa catcgttaaa gggaaggaaa gcatgaaagc
     8461 atcccgcagc actgtaaggt aacgattttg ccctaataaa ttagctaagg gcatgatttt
     8521 ctcagaaagc ttgtccataa aaccgttcat ttttattcac ctcaaattta tttgcaatcg
     8581 attacataca aagtataacg ccaaaaaaat taaatgtcta gtcattttca taaaaagtat
     8641 tgatttattt tttagggttc gttatgatta cggtatcaag aaaaaagaga ggaagtaaaa
     8701 aaatgaaaac ctacacattc ccagaaaatt tttggtgggg ttctgctgct agcggaccgc
     8761 aaacagaagg aagagtaagt gatgatggta aaggagaaaa tatttgggat cattggtatg
     8821 aattagagcc tgaaaaattc ttcgatcaag taggacctga aaaaacttcg caagtttaca
     8881 caaaatacaa agaagatgtt cagttaatga aagaaactgg ccataacaca tttcgtacat
     8941 ctatccaatg gagtcatttg attccagaag ggacaggtga aatcaatcaa aaagctgttt
     9001 ctttttatcg agattttttt actgaattgc tggataatgg tattgaacct tttgtgaacc
     9061 tttaccattt cgatatgcca ctaccactgc aagaaaaagg cggatggtta tcaagagaaa
     9121 cggtcgacgc ttatgtttct tatgcagcag cgtgctttga actgtttgga gatctggtca
     9181 aaaaatggtt tactcataat gaaccaatcg tacctgtaga aggaggctac ttgtatcaat
     9241 tccactatcc aaatcatatc aatatgaaag aagcggtcca agtaggctat catgagaatc
     9301 tagcaagcgc aaaggtaatc aaagcctacc atgaaggcgg acatgatggt gagatcggaa
     9361 tcatcttgaa tctgacacca agctatccga gagatgaaac gaacccagaa gatgtaaaag
     9421 cggcgaagat cgctgacgca ttcttcaacc gatcattcct tgaccctgca gtgaaaggag
     9481 agtttccaca agagttgatc gaaattgtca aagaattggc tataatgcca gagatccatg
     9541 aaggagacct agaactcatc cgtgaaaaca cgattgacct gctaggaatc aattactatc
     9601 agcctcgtcg aatcaaagca aaagaaactc caatcaatca cgaacatagc cccatgccag
     9661 atgattattt tgataattac gacatgccca ataaaaaaat gaatccatat cgtggatggg
     9721 aaatttacga aaaaggaatc tatgatattt taacgaatac aagagaaaac tatggtaata
     9781 tcaaatgcta tatttctgaa aatgggatgg gggtcgaagg agaagaacga tttgttaaca
     9841 gcgaaggaat tattgaagat gattaccgga tcgagtttgt tacggatcat ttgaaatacg
     9901 tccatcaagc aatccaagaa ggaaccaatt gtgtgggtta ccacatgtgg acatgtatgg
     9961 acaactggtc ttggacaaat gcttataaga atcgctatgg ctttatcgct gttgatttgg
    10021 cacaagacgg taaacggacg atcaaaaaat caggtcgctg gtttaaaacg gtctctgaaa
    10081 aaaacggatt tgatgcctga tattttttga taaaacgttc acaaaatgtt tgttctaaca
    10141 ctgtaatcgt gctacactaa cagtgaagga ggcgtttaca atggtagata cttatcgtaa
    10201 tattttagta gcaattgatg gttctgaaaa ggcagaaaaa gcatttcttg aggcaatcac
    10261 gattgccaaa cgaaatcagg caacgttgca catcctgtat gtcaatgaag taacaggcaa
    10321 ttactttggt gattttgcat ttgttacaac aagccttcag gaagaattgg atgaggttgc
    10381 ggaaaaccag atgaaagaac atcgaaactt ggcaattgaa aaaggcctga cagatatcga
    10441 gacatatgtt ctttacggtt atccaaagac actgattgct aatttccatg aatcgaaaga
    10501 accaatcgat ctgattgtca tgggggcgac aggtctgaat gcggtagaac gtgcactagt
    10561 aggttccact acttcctatg tagtcaacca cgcatcatgt aacgtcatgg ttgtaaaata
    10621 aataaattaa gggaacagaa agcaaaaaag cataacgctt cttttgcttt ctgttccttt
    10681 tatttttttg taaaaaatta ctttacctga tgaagtaatc atgctattct aattacaacg
    10741 acagatggag taatatggag gaaagaaaat gatttcaagt gatggaatac gcggatacaa
    10801 tgatgcgatt attttatcga tccttaacaa aaacgattct tatggatatg aaatttctaa
    10861 aacgattcgt gagaggacaa atgattccta tgtgatcaaa gagacaacac tttactccgt
    10921 ttttgcccgt ttagaaaaga atcaactgat tgtttcgtat cctggagaag tgactcatgg
    10981 aaaaaagcgg acatattata aaatcactga aatagggcgt aatcttttag aagaaaaaaa
    11041 gcaagagtgg ctgacgacga aagacgtagt cgatcaattt ttggagggaa attaaatgga
    11101 tacaatccgt aattatttag acagtctttt tatcggagta cctcaaagta ccgagataga
    11161 taagctgaaa actgatttgt tggcaaatat ggaagaccat tatcatgaat tgatgggaga
    11221 aggaaaaaat gaacaggagg ccatcggtac agtaatttcg acatttggtt caatcgatga
    11281 attattagaa gaactagatg tcgaaaaaaa acatcaagct gacgagacgg aaacaaacac
    11341 agcttctatt tatttaagcg aagcagaaaa ctactggaaa gaatacagag cggcaagtct
    11401 ccaagtggca tcaggtgttc tattcattag cttatctttt gcaagttttc ttttcttttg
    11461 ttcagcagga tatgtgttca tgggtatttc ttgtctgatt ttcggtatag cattagctgt
    11521 cggttttttt attgcttctg gtatgaaaat cacacgttta aatcactttt tgcaccatcg
    11581 gaaaatacct gaaaaagtat tagcagaagc aaaagaaaaa gaagaagagt atcagcgaag
    11641 ttttggcttc tcactgattg caggaattgg attatgtatt ttttcacttt tcccactttt
    11701 ggcttcttta atgtggtata tggatggtag tatcggtgcc tctatatttt ttgttacggt
    11761 aggaacgggt gtcttcttga tcatttatgg cagtctagtc cgtcattctt atcgtcaatt
    11821 cactcaaagc gcttattact ggtgaatgta ttcagatttg taaaaatatt ctatttatcc
    11881 ggcgtagaaa aaaagactgt gacacgcttt tgtcacagtc ttttttatat ataataattt
    11941 aaaaattcat catctaaatc cgtagaattt tctagattgc ttacccatgt aaaatcgtta
    12001 tagacagatt tcagttgact tctgcgccat tgtacaggga taaaaagacg catgctatct
    12061 tcattgaatc cgactaataa tttctttccg tcagtaatga tgggacgttg taacaaagta
    12121 aaatgcgaga cgatcagatc gacaagctct gaggtcgtac attggtcgat ttggtctttc
    12181 aaatttttat aatcaatcga gcgtttagct aatagatcgt caaatccgtt ttctgttaat
    12241 gacaataaat aaaatatctc tttccttgtt aaaggctgtt taggtgtgaa aattcgttct
    12301 ctgtaaggca tttgatggtc tttcagccag tttgccatca gatggtaact atgtgtgcga
    12361 gaagaataga tcgtaagcat aaaaaaactc cttaaatagt ccattaattt gttagatctg
    12421 ttgagataaa taataggtaa ggagctgtgc ataagcattg atcaaaacaa cccaaacatc
    12481 attagaaata actttctaaa gtcttaaact tgcgacgaga aacaactaga ctaatagaaa
    12541 aaatgtctta gccatttggc ttcctgtttg tttttttgct tatgctgcag ctcctcggga
    12601 aagggttcta acaatttgaa gaaggttgat gattgatcta gtttaaattg tgtgaaattt
    12661 gaaatctggt gtttttacta gatgaatcac ctaacaacta tgattataac ttatagaact
    12721 tctgttttgc attcggtttt gttatagttg ttataaagtt ttattttatt acagaacatt
    12781 tttaattaat aaagaacagt tagaagttaa aattgctgat tcacatagag tttatgactt
    12841 cttggaaatt ctttttcaat cagttttttc atctgtttat gaaattcttt tttttcagta
    12901 aacataaagg tttgggtatc gtaaccttct tcatgtagga aatagattat tcctttttct
    12961 gtgttttgcg tataagcttt tatactcctg tcttttttga aagaacagat cgtatagcca
    13021 tctatttggt ctggtagtcg tttgattttc ttcaaaatga cttttttatc ctcaagtttc
    13081 atttttttca cctgacttta tttttttcat atggaaatca tagcgccttt ggttatatga
    13141 aaacaagaag gatactgaaa tatatataaa tcaaatagtg gttatttatt attagaatag
    13201 cgaatacaaa taaacgacca ctattttctg atttattgca aatgtttttt caaataaatt
    13261 tgattaccaa gaaaaggtag tggtatattt actgagggat actttgaaaa acatatgata
    13321 gaaacagtaa cagtattatg aaaaatcttt ttataaaaat ggaggaatcg atttgatgga
    13381 agatcagtat gtacaagcaa tgacgatcgc aggatcagat agcgatggaa gcgcagggat
    13441 gcaagcagat atgcatacat tctttacaag aaatgtttac ggtgtttctg tcatgaccgc
    13501 atgtgttgca ggtaattctt acggaatcgg agcaagtgtg acattgccaa cagattttat
    13561 cgacaaagaa tttgaactga ttgcgaagga ctttcaaata cgtgcagcta aaacaggtat
    13621 gctagcagat tcaaagttga ttgagacagt tgtaaaaaat taccaaaaat atgattttgg
    13681 accattagtc gtggatcccg taatcgtaac gaaacatgga aatcttttac tagaagaatc
    13741 tgctttgcaa agcttgaaag aaaagcttgt ccctttggcc gaagtactga cacctaattt
    13801 ttatgaagca gaaaagctga ctggacggac attagcagct gaagaagatt ttgtgcaagc
    13861 agcaaaagaa ctgcaagata tgggtgcaaa aaacgttatg atcaaaggac atcatgctaa
    13921 aaatgcgcag caagaaacag tcaatgacta cgtattgttc gaatcaggaa aatcattttg
    13981 gttaaatgca ccattttatc ctaccgaccg gatcaacgga actggcgatt cgctatctgc
    14041 atgtattgca gctgagttag cgaaaggaaa aacagttgaa caagcgatcc gattggcaaa
    14101 agactatgta aaccgtgcaa ttcgtgaaga aataaatgta gggcacaaat atggccccat
    14161 caatcattgg gcagctggtg aattagagta aaagaaagaa gttgtgaaaa agttgccctg
    14221 catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttaac ttggttggac
    14281 ttattaccgc agatgcaagc ttttgaacac cgtttaagaa agaggtcgtg agcctgccgt
    14341 ccagctccga gtcacaagga acaagttcaa aaaacagctt ctcatgtttg ttgaatattg
    14401 tgacttgtga ccgaggagtt ggcaggagaa caccgtttgt tcggaagaag ggactgtgac
    14461 aagatttttg tcacagtccc tttaatctta gtatattttt ctctacttga ataaaaaaat
    14521 tataggattg gagaaaataa tcgagaaatt tgttgcttga ataatagcca gttagacatt
    14581 tccttgactg tttcagttgt catggatgaa caatgtgtct catcttcttt gaactgagta
    14641 gcaagttctt ctaaaaactc aggatcgtaa atgacagcac ttgtttcaaa attcaatcgg
    14701 tagctacgaa tatcttgatt tgctgaacct accatgcaga tttcatcatc cattatcaat
    14761 gtttttgcat ggaggaagcc gttctcatag acaaggattt gtacattttc cttgatcagc
    14821 tgccgagcat aatattgtgt tgctcgataa ataaaaggat gatccggttt gttgggaatc
    14881 atgattttca catctacacc agaggcagtt gcgattttta aagcagcaat gacactttca
    14941 tcaggaacaa ggtaaggtgt ctgtatccaa acacgttttt tagcagaagt aatcaatttg
    15001 ataaatgaaa gcttgatttg ttccctttcg ttattcggtc cgcttgaaac aagctggatg
    15061 gatgtatcag caagcttaga ttcatctcga tctgcttttt taaagaaata attcaaatga
    15121 tagccgacct ttttttcttc aggtaccgag acgttccagt ctgttaagaa gcgaagctgg
    15181 agcaaagaag aagcggcacc gaaaatccgt atatgcgtat cgcgccaata gccgaatttt
    15241 tttgttgtat ttacatattg attggcaata ttgaaaccac cggtataact aatctttcca
    15301 tcgatcacaa cgatttttcg gtgatcatgg taattcaatc ggaaacgaag taatgccctt
    15361 tgtgaggtaa caaatgtatg gacatgtcca ccatttttga ttagacgatt gaaatctttt
    15421 gcttttgtgc catgagagcc aaatgcatca tataatattc gaacttccac gccttctgcg
    15481 gctttttctt ctaaaacatg taagacttgc tggccgatat tatctgtcac aaatgcgtaa
    15541 tattcgatat ggatcgaatg tttggctttt tttagatctt gaagcagtga atccaatttc
    15601 tcttgtccgt ctgtgagaag agtgacagaa ttcattcttg tcagcggcat acgatttaat
    15661 gaagagaaga agtcaacaaa ttgctgtttg tctttatcgc ccatgtcagg atcgtaatgt
    15721 tcaatggtat ctcctctaaa agactgaaag ttttctaatt ctttcaagtc actttgtcgg
    15781 agatagaatt ttttcttatc cgttaatcca cgtccgaaaa ataaatataa tacaaagccc
    15841 accccaggaa gggcaaacaa tactaagagc catgcccaaa tggctgctac atctcggggt
    15901 ttgatcaaaa tagccaccaa agcaataagt gcatttaata gataaagggc ggttataata
    15961 ctagatacca caaaaatgtt cctccttaca tacaataaca ttagtatagc atgatagaag
    16021 aaagaagcct accaaggtga atatactttt cactcctttc atgaaaatgt aggtaaatcg
    16081 ggctaaagta gttgcttttt gtaggttaat aaggtaggat attagtacag ttgaatatct
    16141 ccttagggga gtaaccggca acgtttgttg cgataatatc aacagtaagt taaccaaatg
    16201 gttttctggt attgtatgaa atggtgagac ctaagacaaa tgcgtatttg tcttaggtct
    16261 cttttttttc agacaggact agggccaaga tcaaaaaatg ggggatgaaa tttggaaaac
    16321 aaggaacaag cacaacaagc ggtctataaa gaaacgatcg actcgttgat gcagcaaacg
    16381 cagagctcaa ccgatggatt gagcaccacg caagcgaagg aaaaattaga aagagatggc
    16441 ttgaaccagc taaaagaaaa gccgaagaag ccaacttgga aattgttctt agaaactttc
    16501 aaagatgcaa tggttatcgt attgctgatt gtcgcactga ttcagatggt catgggtgac
    16561 tacatcgaat tcttagttat ttttgcagta ttgatgttga attctataat cagtgttgtc
    16621 caaacaaaaa aagcagaagg ttctttagaa gcgttgaaaa acctttcagc gcccgatgct
    16681 cgagtgattc gtgatggtaa ggaaaaaacg attccggcaa acgaacttgt ggtaggagat
    16741 atcgtgttgt tagacgcagg cgattatgta cctgctgatg gacgtttact agatgcggga
    16801 tcattaaaag tagatgaagg tatgctgaca ggagagtcga cttctgcaga aaaagaagta
    16861 acggatatca caagtacggt accaatcgga gatcgtacaa atatggtatt cagtggaacg
    16921 atcgtaactt acggacgcgg taaattctta gtgacttcaa ctgggaataa tacggagatc
    16981 ggacaagttg ctggcctttt ggaatcaaca actgagcaag tcactccgtt acaacgaggt
    17041 ctggataaat tcagtaaaaa actaagtttg gctattttgg cattgtcttt attgatttta
    17101 ggtatccaac tgtttcgtat ttatcttgga gaaggtacag gagatatgac tgcagatatc
    17161 gtcagcgcag tgatgtttgc cgtagccgta gccgtagctg ctattccaga agcattacag
    17221 tctatcgtaa caatcgtgtt atctttggga acgaataaaa tggcaaaacg tcatgccatt
    17281 attcggaagc ttccagcagt tgaaacactt ggttcgacaa gtatcatctg tacagataag
    17341 actggtacat tgacacaaaa caaaatgaca gttgttgact actttttagg taatggcgat
    17401 actggggatt tcacaaatga cccttcaaaa tggacagctg acgaacgtcg cttgatcgaa
    17461 atcagtgttt tagcgaatga tgcatcgatc agtgaagacg gtactaaatt gggcgaccct
    17521 acggaagtag cgtttatcga ctttagtgaa aaattaaacc agccttatca ggaaatccga
    17581 aacaactacc caagacaagc agaacttcct tttgactcag atcgaaaatt aatgtctact
    17641 ggacacacaa ttgataacaa aaattacttg tttgttaaag gcggaccaga tatcgtcttc
    17701 caacgcagta aaaaagtatt gctaaatggt gaagtcgttc cgatgacgga tgaattgatg
    17761 aaaaaattcc aagagcaaaa tgaagctttt tcaacacgcg cattacgtgt actagctttt
    17821 gcttaccgtc cacttgataa ttttgattta tcatttgaag atgaagacga tttgatcctt
    17881 gttggtttgc ttgctatgat cgatcctcca cgtgctgaag tgactcaggc ggtaacagaa
    17941 gcaagaagtg caggaatcaa aacaatcatg attactggtg accacaaaac aacagctgtt
    18001 gctatcgcaa aacaaatcgg gatttttgaa gaaggaaacc tggctttgac cgggacggaa
    18061 ctagatgaat tgacagaaga tcaactaaac gaagttttag aaaaagtcac tgtttatgca
    18121 cgtgtttcac ctgagaataa aattcgtatc gttcgtgctt ggcaaaataa aggtcatatc
    18181 tcagctatga caggtgatgg tgtaaatgac gcaccagcat tgaaacaagc agacatcgga
    18241 attgctatgg gaacgggtac agatgtggca aaagatgcag cagcgatggt attgacggat
    18301 gataactttg cttctattgt cagcgcagta gaagtaggac gtaatgtgtt tgataatatc
    18361 aagaaagcta tttcttatct atttgcagga aatttaggtg cgatcattgc gatcatcttt
    18421 gccttagttg ctgactggtc caatccattt acagcattac aactgttgtt catcaattta
    18481 gtcaatgatt cactgccagc aattgcatta ggtacagaaa aagctgaacc tgctacgatg
    18541 aaccgcccgc caagagatcc aaattcagga atctttacgg acaaaacatt gatttctgta
    18601 acgtatcgtg gtgtgctgat tgggattgct gttatctttg ctcaatggct tggcatgcag
    18661 cattcacctg aattcggtgt agccatgtca ttttcaacat tgatttggtg tcggacattg
    18721 caaacattcc cagcacgttc aaacacacaa acagctatcc aagcagggtt cttctctaac
    18781 agaagtgtct tactagctgt agttgtatgt agcgcgcttt actgcttaac aatgatccca
    18841 ggactgaaag aagtcttctc tatcccatca gcatttggat tgacagaagt ctttacatgt
    18901 atgggaatcg cacttgtgac aattgttttg atggaaatca ctaaattgat cattgtacga
    18961 gtacaaggaa ataaataaaa cgaatcaacg aggttgtgag cctgccgttt aattctgagt
    19021 cacaaggtgc aatttcaaca aacagcttct catgtttgtt gaatattgtg acttgtgacc
    19081 gaagagttgg caggagaaca ccgtttaagg aagaagttgt aaaatcagca ttttgagcta
    19141 atactgaggg tcatctgatt ggacactgtt tattcggaaa gagggacttt gacacgactt
    19201 tggtcacagt cccttttttt atttatgata gacggaaagc caatcatttt attattcatc
    19261 aaaagagata gaatagataa aagaggagaa tgatttattc cgaaaagtga aaacatataa
    19321 caaaaaatta gaggaaatca aatgaatgaa aaagaatggg atgaatttgc tgaagaatat
    19381 tatgaaatcc agcaagaatc gcaagtaact attgctgaag atgtaaaaaa ttatctgaaa
    19441 acggagaaag tcctgcctgc tgaaacagca gcagacgttg ctggaggatc tggcagattt
    19501 ctttccttag caaaagaaat taatcgctat gatttgatcg atttttcttc aggaatgcta
    19561 gaaaatgcaa aaaaagaagc agaaaaaaat gagttgccaa atgtttcctt tatcaaacag
    19621 tcgttagaag aatttatcca atcaaatgag acatatgaaa tggtttttac agcagcaaat
    19681 ccagcattgg tacatcttat tcaattagaa aaattgatga aaaagtcaag caaatggtgc
    19741 gtgataatca gagtagtgga atcaatcgat gatgtttttt atcctatcga aaagcaacta
    19801 ggaatgatag aagaagatcc taatacatcg ccagttgtaa tggatcaatt tgaaacatac
    19861 ttaattgatg aaggattcaa agtgaagaaa aaagatttta cttattttac acaggaatca
    19921 attactgaag aattgatgaa gctatactat gacgcgtacg caaaagatcc tgttgtgacc
    19981 aggtacattc gcaaactatt cagtgaaaaa cgagaaatcc ttagtacgac taagctgacc
    20041 tatagactgc tcctttggtc cgcagataaa aaaagaacct ttcacgactc gtgaagggtt
    20101 ctttttatga ttttattata ttttgtagta acgaatatca ttttcgatct ttgtaatcga
    20161 aaaaggtaca tctgaagtat attctgcaca ttctccgtgt tcaggaagcg tctcactgaa
    20221 aatcgtttca tattcgtcaa cagtgatccg tgttcgagaa tctagcatct cttgatgaga
    20281 ttgtgcgaag agatattctt gatagttttc ttccagataa cctgtaaaaa attcacttac
    20341 tgcaccagaa ccgtagctga atagaccgat gcgatctcct ggctgtagtg atttcgaatt
    20401 ttccagtaga gaagttagcc ccaggtataa tgaaccagtg taaagattac cgattcgtcg
    20461 gctgtaacgg atgctttctt catagcgagc cataagacgt tcctgatttt cttcgtctgt
    20521 ttggtctaat acgctttgca atgccttttt tcccatctta gtatacggaa tatggaaagc
    20581 aatcgcctga taatcttcga gtcctcgacc cgacaattct ttatgtcgat tccaaacttt
    20641 ttggaatgat tcgatatacg tagaattaga taaaggacca tcaacaacag gaaattcgct
    20701 ataatctgga cgccagaaat catagatatc ttctgtcaga aatacgctgt cgtcttcaat
    20761 cgataaaata cgcgggtttt gagtgatcat catcgcaaca gcaccgacac cttgcgtcac
    20821 ttcaccaccg cttgccaagc cgtaacgagc aatatcactt gctatgacta gtacttttcg
    20881 ttctggatga tttttgacat attctttcgc catgtgcaaa gctgcggtgc caccgtagca
    20941 agcctctttt acttcaaaag aacgagcaaa agattggatt ttcagtaaat ggtggataat
    21001 taccgcactc gcttttgaat gatcaatccc ggattctgtt ccgacgatga ccatgtcgat
    21061 tagctcacgg tctttttctg ttacgatttt gctggcagca tttgctccta gtgtaatgat
    21121 gtcttcattt gcacgattga ctgccatttg gtcttgacca atccctatat ggtattttgc
    21181 aggatcatcc ccacggcttt ctgccaactc tgtcatatct aaatataaat taggaataaa
    21241 aaaggaaaga cgatcaatcc ctattttcat ttcaacacct ctttacttat gataaaaaac
    21301 ttactttact aaggtatcat aaaaagatta aaagaaatat aaaaaagaga atatttaagg
    21361 attgttaaaa tttaatctta gaaaaaaata actggaacaa gagctatttg tttttacagc
    21421 aaagaatggt atatttttgt aggaggtggc ttacttgaaa gaagtcgtta tgatagatgc
    21481 tgcaagaacg ccgataggaa agtatcgcgg aagtttaagt ccatttactg cagttgaatt
    21541 aggcacactt gtgactaaag gattattaga taaaacaaaa ctgaaaaaag ataaaatcga
    21601 tcaagtaata tttggaaatg tcctgcaagc tggtaatggt caaaatgttg ccagacagat
    21661 tgctttgaac agcggtctcc ctgtagatgt cccagcaatg acgatcaatg aagtttgcgg
    21721 atctggtatg aaagcagtga tcttagcacg ccagctgatc caattaggtg aagcagaact
    21781 tgtgatagcc ggcggaacag agagtatgtc tcaagcacct atgctgaaac agtatcagtc
    21841 agaaacaaat gaatatggtg aaccaatttc cagtatggtc aacgacggat tgactgacgc
    21901 attttcaaat gcacatatgg gattaaccgc agagaatgtt gcaacacaat tttctgtgag
    21961 cagagaagag caggatcgct atgccttgtc gtcccagttg aaagcagcac atgctgtcga
    22021 agccggtgta ttttctgagg agatcatccc agtcaagatt tctgatgaag acgtgttatc
    22081 tgaggatgaa gcagttcgtg gaaatagtac attggaaaaa ctgggcacgt tacgtacagt
    22141 attctcagaa gaaggaactg taacagcagg aaatgcttcc ccgttgaatg acggtgcctc
    22201 tgtggtgatc cttgcatcca aagaatacgc agaaaataat aatctgcctt atttagcaac
    22261 catcaaagaa gtagcggagg tcggtattga cccaagtatc atgggaatcg ctccaatcaa
    22321 agccattcaa aaattgacag atagatcagg aatgaattta tcaacaatcg atctttttga
    22381 gatcaatgaa gcatttgcag cttcttctat cgtagttagc caagaactgc agttagatga
    22441 agaaaaggtc aatatatatg gaggagcaat cgcattaggc catccaattg gtgcaagcgg
    22501 tgcacgtatc ttgactacat tagcttacgg attgcttcgt gaacagaaga gatacggtat
    22561 tgcttcatta tgtattggtg gaggtttagg cttggcagtc ttgttagaag cgaatatgga
    22621 gcaaacacat aaggatgtgc aaaaaaaaaa gttttatcag cttacgccat ctgaacggcg
    22681 cagtcaatta atagaaaaaa atgtattgac acaagaaact gcgttgattt ttcaggaaca
    22741 aacactttct gaagagctct ccgatcacat gatcgaaaac caagtgagtg aagtggaaat
    22801 cccaatgggg atcgcgcaga attttcaaat caatggcaag aaaaaatgga ttccgatggc
    22861 aacagaggaa ccatctgtca ttgcagctgc aagtaatggt gcaaaaatct gtggaaatat
    22921 ctgcgcagaa acacctcagc ggctgatgcg agggcaaatc gttctatcag gaaaatcgga
    22981 gtatcaagca gtgatcaatg cagttaatca tcgaaaagaa gagctgattc tttgtgcaaa
    23041 tgaaagttat ccttcaatcg tgaaacgcgg gggcggtgtt caagatatct ctactcggga
    23101 attcatgggg agtttccatg catacttatc tattgatttt ttagtggatg taaaagatgc
    23161 aatgggcgct aacatgatca actcgattct tgagagtgtg gcaaacaagt tacgggagtg
    23221 gttccctgaa gaagagattc tattcagtat tttaagtaat tttgctacag aatcattggc
    23281 ttctgcctgt tgtgaaatcc cattcgaacg cttagggaga aacaaagaaa tcggtgaaca
    23341 aattgccaaa aaaatccaac aggcagggga atatgcgaag cttgaccctt accgtgcagc
    23401 aacgcacaac aaagggatca tgaatggtat agaagctgta gtggcagcta caggaaatga
    23461 tacacgtgcg gttagcgctt cgatccacgc ctacgcggca agaaatggat tataccaagg
    23521 attaacagac tggcagatca aaggggataa actagttgga aaactaacgg tgcctttggc
    23581 tgttgccaca gtaggcggag cttccaatat cctgccaaaa gcaaaagcat ctttagctat
    23641 gttagatatt gattcagcca aggaactagc acaagtaatt gctgcagtag ggttagctca
    23701 aaacctggct gctttgcgtg cattagttac agaaggaatc caaaaaggcc acatgggact
    23761 tcaagctcgt tcattagcga tctcaattgg ggctattggt gaggaaatcg agcaagttgc
    23821 gaaaaaatta cgtgaagcag aaaagatgaa ccagcagaca gctatacaaa tattggaaaa
    23881 aatccgggaa aaataaaaaa gcaatatctt tagaagaata aagcaagatt tacagttatt
    23941 ttatttactg cagatcttgc ttttttctct atcagaaaat atgtttatta ttagaatttt
    24001 ttatagttaa ggttaggcat cttaagaaat caggcaactc caagatatct tcaggtagaa
    24061 gagaagtcgc ctaacattgt aggatcatag gtgatttgtt ggacaggtca agctgatatt
    24121 tgagcctgat gttagaagga atttcttttt acttgctgta gaaattatta tatgactaaa
    24181 aaggctggga ttttaaatcc cagcctttta tgacaaatag aatttttatc gatcagtaat
    24241 cgctgtcgct gcaaattgtt cgatcaaagg aatcaaagtc aatttgactt tgtatttccg
    24301 ttcgagtgct ttttcgataa cgaactcatt ttcagtagtc atagaaatag ttgctaattt
    24361 acgaggttca tcagcatttt tttcattaat aactacttca tgttcatttt gttctgtgta
    24421 aaatttagga tactcagcaa cggcttttga aaaatcagca aaacttaaag cggcattcgt
    24481 atcagttgag cgtgtatttt cagttgtgtc cgcttttggt ttttccgttt tttcttctgg
    24541 cacactgact gtttcttctg taactggtgt cgcaacagct tgcgcagttg gttcatcgtt
    24601 tttcaatcgt tgatcattcc agtaagacac gatttcaata agaagattgt cacggaatcg
    24661 ttcgaaaatc ccgttttctc ctgaacgaat tctctttaat acagtattga agttgtcatc
    24721 gattggcacg ttgatccgtg tatcttcatt tgttttccca gctaatccga tcgttaagat
    24781 ttgacggttt ttgggatcaa gcatgacttg taacggcaaa ccataacgtc gcaagctctc
    24841 ttcttctaga gaacggaata attgtcctct tacaccatcc aatgaaggac ggaattgatt
    24901 taataatttt tgtttctgtt ttttcttcat atgttctctc cttcgctcat accctttcga
    24961 ttataagaaa aaaacagcca aatgaaaagg agtaatgcaa aaaaacttgc gtatacgcaa
    25021 attacaacag aaaaatttgc gtattttccc gttttttgaa agaaaaataa aaaatggaac
    25081 ggattatgta tatatacata atccgttcca ttctttctat tttttcggta tcaaggcaaa
    25141 aacaggtagt tcaggtgaat aaatgacttc atgttcgtgc cagggtttga ccattagcga
    25201 atgatcgaac cgttttttca gttcttcgat gatttgatca tgtgtccagt ttcttgcagg
    25261 aaaatgttgg attttctctg catcgatcaa aaagacaaaa tcggaatgaa aagtctgggt
    25321 aacttcttcc gagagagaat tcaagcgttc gattggatcc ataaaatcac ctttcgactt
    25381 aaaattgggt agtatctggg tcttttgctt tgccgactac accatctaac tgatcgattt
    25441 gtttcatttc ttcttctgtt aattcaaaat caaataattc agtattttct ttgattctgc
    25501 ttggtgtcac tgacttagga agaggaagga attcgtgttg cagcgaccaa cgcaaagcaa
    25561 cttgtgcaat cgtcttgtca tgtgcttcag cgatttgttt catttcaggc acatcgaaga
    25621 ttttaccagt gcctaatgga ctatatgctt cgaggatcat atcatgtttt tttgcatact
    25681 caactacagt tgtctgtaat tcgcctggtg caaggaaaac ttgattgacc atcggcataa
    25741 tttttgctgt ttttgctagt gtatctagat ggtggggtaa gaaattgctc acaccgattg
    25801 ctttgatttt acctgcttca tacagttctt caaatgctcg ccatgtatct gcatttgctt
    25861 gttcccaatt atcacgaaaa gcaacagggt ttggccagtg gatcaaaaac agatctaaat
    25921 aatctgtttg gagcttcttc aatgattctt caaaagaaga catcacaagt tcatagctgt
    25981 ggtttgcatt ccataatttt gttgtcaaga atatctcttc tctaggaatt ccactttcct
    26041 taatcgcttg gccgacgctt tcttcattct tgtatccttg ggcagtatcg atatggcgat
    26101 aacctgcagc cagcgcttct ttaacagagg aaacagcgac atccccatca ggtgtttgcc
    26161 atgtgccgaa tcctactacc gggatttcgt aaccatttga tagacgataa gtgcttgtta
    26221 atgaattcat actttcagct ccttttttat tcgttcaact ataagtgtag cgcatttttc
    26281 gaaaaaaaga atccattttg tttctactct tgaaattttt gtttaaaaga aaatgatttt
    26341 gattctggta tttttgattt tttttccgta aactaagaca gaagggaatg agaaaatgga
    26401 tatctattta aaaaaagcaa ttctgcatat tattgacaga gaaactggtt ctccagtctt
    26461 ttcacaaaaa gagctggatc tgacaaaaga atatattcgt gattttcttc aaaagaagat
    26521 ccaaaaaatc tcttctgctc aaacaaaaac tggacaacta gcagaagaaa gcatgatcgt
    26581 tgaatcactt aaaatcgctc aagaaaattt tgtggaagca agtgaagcaa tcgttcagcg
    26641 gtggttcgat atctaccaag aaagtgaaga agcacctagt gcagatgtat ttgttgtctt
    26701 gtatgaatta gatacagtga tgtacttatc tttattgaaa gtcaattatc gtgaggctta
    26761 cactcacttt gttgaagcag acgaagcagg gatcgacaac cgcttgattt tgaatcgagc
    26821 aattctaggg gcgaaaacac aaaaagcaga tgaagcattc gctgtgaatt tgactgattg
    26881 gacctacgaa ttaattgaaa aaaaatatga gttttctggc aaaaaagagc tttatttttc
    26941 tggaagagtg atcgaaagtg ttcctgcacc ttctttagaa gaaaatgtaa aagtgatcca
    27001 aaaagtagcc aagcatcttg gaaaaaaatt tgagacagaa gaatttgata tcatggctga
    27061 tttgaaagaa gctgtctatg atacgataga agaaaaaggt cggttggatc atgagatgat
    27121 tgcggaaaaa gtcttcaagg aaaacatcac ggcaaaaatg gcttttaaag aagaagtaca
    27181 agagcaagga tttgtcccag aagcacctcc tgtaaaagaa gtacaagaaa tttctgagaa
    27241 aaaatttgga aaacaaaaat tcaagctatc aaacgggatc gaactgatcg taccagtaga
    27301 tgtatatcgc aacccagatt tgattgaatt tgtgaataat ccggatggta cgatatccgt
    27361 gatgatcaaa aatgtggatg aagtattgaa tcgtttataa aaatgactag aatattaggt
    27421 gttcaagtac cagtgatcag ttaaatgaat tgtgtcaaga gaaggatcgg aaaagacgaa
    27481 tataa
//
LOCUS       DACDRN010000032        27303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDRN010000032 DACDRN010000000
VERSION     DACDRN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.032"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(38..634)
                     /locus_tag="IWJ80_001599"
     CDS             complement(38..634)
                     /locus_tag="IWJ80_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9461316.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            984..1328
                     /locus_tag="IWJ80_001600"
     CDS             984..1328
                     /locus_tag="IWJ80_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP9461317.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1329..1748
                     /locus_tag="IWJ80_001601"
     CDS             1329..1748
                     /locus_tag="IWJ80_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP9461318.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1840..2190)
                     /locus_tag="IWJ80_001602"
     CDS             complement(1840..2190)
                     /locus_tag="IWJ80_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461319.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2156..3487)
                     /locus_tag="IWJ80_001603"
     CDS             complement(2156..3487)
                     /locus_tag="IWJ80_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP9461320.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3742..4308
                     /locus_tag="IWJ80_001604"
     CDS             3742..4308
                     /locus_tag="IWJ80_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9461321.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            4453..4540
                     /locus_tag="IWJ80_001605"
     tRNA            4453..4540
                     /locus_tag="IWJ80_001605"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4485..4487,aa:Ser,seq:cga)
     gene            4876..5850
                     /locus_tag="IWJ80_001606"
     CDS             4876..5850
                     /locus_tag="IWJ80_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP9461322.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            6175..8070
                     /gene="asnB"
                     /locus_tag="IWJ80_001607"
     CDS             6175..8070
                     /gene="asnB"
                     /locus_tag="IWJ80_001607"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP9461323.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(8265..8711)
                     /locus_tag="IWJ80_001608"
     CDS             complement(8265..8711)
                     /locus_tag="IWJ80_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9461324.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8939..9088
                     /gene="rpmG"
                     /locus_tag="IWJ80_001609"
     CDS             8939..9088
                     /gene="rpmG"
                     /locus_tag="IWJ80_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9461325.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            9176..9694
                     /locus_tag="IWJ80_001610"
     CDS             9176..9694
                     /locus_tag="IWJ80_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9461326.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9771..10727)
                     /locus_tag="IWJ80_001611"
     CDS             complement(9771..10727)
                     /locus_tag="IWJ80_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9461327.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10893..11699
                     /locus_tag="IWJ80_001612"
     CDS             10893..11699
                     /locus_tag="IWJ80_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9461328.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            11696..12685
                     /locus_tag="IWJ80_001613"
     CDS             11696..12685
                     /locus_tag="IWJ80_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9461329.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            12682..13686
                     /locus_tag="IWJ80_001614"
     CDS             12682..13686
                     /locus_tag="IWJ80_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9461330.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13818..14360
                     /locus_tag="IWJ80_001615"
     CDS             13818..14360
                     /locus_tag="IWJ80_001615"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP9461331.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            14380..15078
                     /locus_tag="IWJ80_001616"
     CDS             14380..15078
                     /locus_tag="IWJ80_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP9461332.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            15099..15311
                     /locus_tag="IWJ80_001617"
     CDS             15099..15311
                     /locus_tag="IWJ80_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP9461333.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            15311..16273
                     /locus_tag="IWJ80_001618"
     CDS             15311..16273
                     /locus_tag="IWJ80_001618"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP9461334.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            16291..16686
                     /locus_tag="IWJ80_001619"
     CDS             16291..16686
                     /locus_tag="IWJ80_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9461335.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16849..17214
                     /locus_tag="IWJ80_001620"
     CDS             16849..17214
                     /locus_tag="IWJ80_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP9461336.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            17211..19022
                     /locus_tag="IWJ80_001621"
     CDS             17211..19022
                     /locus_tag="IWJ80_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP9461337.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(19084..19251)
                     /locus_tag="IWJ80_001622"
     CDS             complement(19084..19251)
                     /locus_tag="IWJ80_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP9461338.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            19500..20489
                     /gene="galE"
                     /locus_tag="IWJ80_001623"
     CDS             19500..20489
                     /gene="galE"
                     /locus_tag="IWJ80_001623"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP9461339.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            20501..21985
                     /gene="galT"
                     /locus_tag="IWJ80_001624"
     CDS             20501..21985
                     /gene="galT"
                     /locus_tag="IWJ80_001624"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP9461340.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            22009..22989
                     /locus_tag="IWJ80_001625"
     CDS             22009..22989
                     /locus_tag="IWJ80_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9461341.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            23078..23902
                     /gene="tsaB"
                     /locus_tag="IWJ80_001626"
     CDS             23078..23902
                     /gene="tsaB"
                     /locus_tag="IWJ80_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP9461342.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            23886..24428
                     /gene="rimI"
                     /locus_tag="IWJ80_001627"
     CDS             23886..24428
                     /gene="rimI"
                     /locus_tag="IWJ80_001627"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9461343.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            24435..24899
                     /gene="rimI"
                     /locus_tag="IWJ80_001628"
     CDS             24435..24899
                     /gene="rimI"
                     /locus_tag="IWJ80_001628"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9461344.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24896..25912
                     /gene="tsaD"
                     /locus_tag="IWJ80_001629"
     CDS             24896..25912
                     /gene="tsaD"
                     /locus_tag="IWJ80_001629"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP9461345.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(26484..27185)
                     /locus_tag="IWJ80_001630"
     CDS             complement(26484..27185)
                     /locus_tag="IWJ80_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9461346.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         8871 a         4693 c         5641 g         8098 t
ORIGIN      
        1 acattgtcaa tcaatgacta attttacaaa ataaatatta tccaagaaga ccacttagat
       61 attgacctcc ataaatcaag gccatactat ataagaaaat cgacagtggt ttagcaatca
      121 aaatgattag tgtatatcgt ttcactgaca tgtttgtcaa tccagccatc aaacataacg
      181 catcgtcagg agcaactgga agaaaaatag ctagagcaaa cagcttctca aaacggtttt
      241 gattatccag ccagccaata tatttgttat atgtcttatc gctaatcatg gaaagtatga
      301 atggctttcc ataacgacgc cctagcagaa aaatgataat cgaaccaatg cagattccaa
      361 tatagttata gataaatcca gcataagggc caaatatcag gacaccagct gcagtactga
      421 tccctccagg aatgattgga ataactactt gcaggatttg gatgaagaca aaaataatcg
      481 gtcctaaaat gatggaatca cctactaatc ctcttaacgc attcaaatct ttgaaaacac
      541 ctaaattaat aaaataaatc gtcagcgcga ttgataggcc taatccgata atggatatga
      601 aatttatcag ttttcgagag gcagcaatat tcattattca tcaactcctt agaggttctt
      661 tgttaactat actttactag aaaagcaact aactgcgcca ttttttgata tgacagaaaa
      721 caaacaattt tgtaagttca tcataagtat aaaagattga tctcaaaaaa acatccaact
      781 aaagacttat ttaataactt gtttttttta aaataaaaaa agcgttagca actatgctta
      841 aaaagcatat cgactgaata aactaagcat tggacaggct gaaagactac atttaaaatg
      901 aaatgcgaag aaacaataga aaataaagtt gccttagaac catagttgaa tcacctaaca
      961 gtcgtgaagg gagaataaga gaaatggcag aaaaacaaac cgcaggacga gatcaattag
     1021 gagattttgc accaaaattt gcagaattaa atgatgatgt tttatttgga caagtatggt
     1081 ccagagaaga attatctgct catcagcgta gtatcatcac catatctgca ttaatttcag
     1141 gaggaaattt tgaacaattg ccagctcatc tgaagattgg caagcaaaat ggcattacta
     1201 gtgaagaaat agcagaaatc attacccatt tagcttttta tgtagaatgg ccaaaagctt
     1261 ggtcagcttt tagtttagca aaagaaatct atttagaaga aaatagcgaa gataagaaag
     1321 aggcgtaaat gatgacaaaa cacgaagaaa tacacaatgg cgtgattttt ccagcaggtg
     1381 aaaaaaacga agcttacgct caagtattta ccgggcaaag ctatctgaat atgttagtga
     1441 atgatcccga agtttctgtt ggtgtaggta atgtaacttt tgaaccaggt tgtcgcaatc
     1501 actggcatgt ccatacagac ggctatcaaa tcttacttgt gaccggagga gaaggctggt
     1561 atcaggaaga aggaaagcca gcaagacgat taatggctgg agatgtcgtc gtaacgcaca
     1621 aagggattaa acattggcat ggtgcgacaa aagacagttg gtttgaacat ttggcgatca
     1681 ctgctggtaa ttcagagttt ttagaacctg taacagatga agtgtatgaa ggattggaaa
     1741 atcaataatg tcaagcttga acagctcctg aaagtagatt ttaatcgaac tttcaggagc
     1801 tgcttttttg attcagtctt ttataaatag aaaatagggt taataatagg aatgagaagc
     1861 acgtcgaaag atcttaatat tgataatcgg ctgggcatac atgatttcca cgtcatcaat
     1921 caaactacct aacagatgat aatcttcccg atcatgtcgg ggattaccta atagttcttg
     1981 atagtagatt tccatactag ggtcccgagg attgttcagc aaatcagcaa gtacatctaa
     2041 atcttctggc tgagtttctg ccgtaccctg tatctctatg aaaagccctt cctcagcaaa
     2101 ttctactcca atacgtattt cagttatttc atgggaatat aaataagtaa gaagctcatc
     2161 gatgattctt tttgataatt ctttttccaa gtgtcttttt ttcctttctg acagcggtaa
     2221 agttatctag tatcggtaca aggatacctg caacgaaacc agttgaaaaa ccactgttat
     2281 atagatttag tcctccgtgc aaagtgctga cgttatggac aagagcaata tgtagcacac
     2341 cagctattac accgtaaaaa ctgccgtaaa aaccgcttat gggcgcaaga cctgtcccaa
     2401 acacagctgc aacaagcatg ccagtactgg tgatctcatg aaaagttcca aacaaagaag
     2461 caataaatat tccgactagt atcggaaaac tgttttttaa atgacaacca aaagcactga
     2521 aacccaccgc agataaaata gcaccgatga caggtccatt taactgaccg ccaaccagca
     2581 aaacataaga agacagcatc agaccaacta agcccatgtt gatcaaagta gcaccaatac
     2641 cagaattagc aatgaaatca gttgtcaatt ttccagatga atcgaaaatt tctcgaatcc
     2701 ccgaaagccg aaaggaatta taatagaaac caattccgac tgtcagcgaa aaaaagccaa
     2761 aaataatcca aatgagagga aagtgatatt cattgaaaac caatgtattt tcgacgattg
     2821 aatagctgaa taacctcaaa acagcagtaa aagtcatcgc aatcaaccca gaggtgaatc
     2881 caatattata aagtgtgaat ccacgatgga aaactaaagt ttgagcagct aagggcggaa
     2941 gaagtatacc aattgctatg ccagctaaat tccccaataa aatacctatc agtaaaggaa
     3001 aacgcatacc aaaagtgata taactaacga ccggtgataa tacgctacca aacagtccga
     3061 tcatgatgta ctgagaaaaa ggtttattga caaatacagc gtatagatac acacctaaaa
     3121 tgattgagat agaattataa aggtttttgc caaaaaaaga gaaaccggta actgttagaa
     3181 tcgaagcaaa taagggaccg tttaaaatca ccttgtgccg ataagctaaa agcacagaaa
     3241 gtaaggtcaa aatccctaca tttatgaatg cgcttccgaa aacacctaaa gcaaaataat
     3301 ctgtcaaaag attgcttggc gaggtaagaa tagttagcaa tccactccaa ttatcagtag
     3361 gagtattaga aagcaagcca ataaaaagaa aaaaaacacc tgtaaaacca agataaaaat
     3421 actttctttt ctgtgaaacg attcgttttt gaggatgaat ataaataacg tcagtttcgt
     3481 tttccattac acaccttctt tttcattttc tttaactata acttgaaatg aaagatttgg
     3541 acaactatca tttaggtcaa aactaaaaat gtaagggaac ccattgaatt taaccaaatt
     3601 tttttaattt tgcaaaaaaa agttaaagaa gactgaaaaa aatttaaaat ataacgtttt
     3661 gagtaaaatg tttgcaaaaa atgctaaaaa tagggtacaa taaaacaaca tatactgcag
     3721 taaagaagtg ggggagtttt aatgagaaga aaagtctata ccaaggatca aattctaaaa
     3781 gctgcttatg atgttgttgc aaaggaaggg tttaaaggat ttactgcgag aaatattgct
     3841 aaaaagatgg ggatttcgac acagccgatt tatctggaat tcaaaaacat ggaagattta
     3901 aaaaatacgt tgctggattc tatctttgaa caactaaaag aagatgtttt ccctattgaa
     3961 cgtactggtg ataaaatcat tgatctcggt ttgaactata tctactttgc aaaaaagcat
     4021 catagtcttt acatggcatt gtacattgat gaacatgggg acggaaagaa aatgcatgat
     4081 ttttcataca attatttccg tgaaatagtt aaagaagatg aaagatacaa tcaattatcg
     4141 gatgaatatg tcgatgctct gcatactggt acatgggtag cagtgactgg cttagcgtca
     4201 ttgatgtcat caaacatcat gcatccaaca gaagaacaaa tcattgcact gatcaaacgg
     4261 acaattgact cgattttagc attagataat cctgaaataa tcagatagtg ttctatcagt
     4321 atgaatttga tcggtaagtc cgttggctgc aaactgtcaa tggacttttt tgctaaaaaa
     4381 agcgatttct gttatttatc tattagaaat aggcttgaca tttcgtgaaa aaagtttaaa
     4441 aatagtactt acggaaggtt ggcagagtgg taatgcaccg gactcgaaat ccggcgaacc
     4501 ggctaatacc ggcgcgcagg ttcaaatcct gtaccttcct tatatcaacg attagaggtt
     4561 gtgaagaagc tgtcctgcat caagtaataa gaacggccaa acaaaaatag cttttcctat
     4621 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttgttcgga
     4681 ataaggggct gtgacaagac ttttgtcaca gccccttatt gttgtacttt taacctacaa
     4741 tattttttga ttccccctga gtactatttt ttataaatgc tagtaaatca atcttctttg
     4801 gccttttttt tgataaaaaa aaatactaga tgtaagataa aagtgtaaaa acgatttgtt
     4861 ttaggaggga aaacgatgtg taccgcaatc acttatgcaa cgaaagacca ttattttgga
     4921 aggaattttg attatgagat gtcttataat gaagtagtca ctattactcc aagaaattat
     4981 cgtttcgatt ttagaaaggt aaaaaattta gataaacatt atgcaatgat aggaatagca
     5041 gctggggtat ccaactatcc actctattat gaggctacaa atgaaaaagg gttgagtatg
     5101 gcaggactga attttcctgg caatgctgat tataaagaat tacaagaagg aaaagataac
     5161 gttgcaccat ttgagtttat tccatggata ctagggcaat gctcaaccat agacgaagct
     5221 aaagaattat tggctactat caatctagtc aatatcgatt ttagtgaaaa actacccttg
     5281 tcacctttac actggttatt agctgataaa gaaaaatcga tagtaattga aagtatgaaa
     5341 gatggacttc atttatatga taatcctgtc ggtgtgttga caaataatcc gccatttgat
     5401 tatcaattat ttaatctgaa caattatcgc tcgctatcaa atggaacacc agaaaatcat
     5461 ttttcaaacc agatcagctt gaatgtttat agtcgtggga tgggaggatt gggcttacct
     5521 ggagatctct cttctgtttc tcgatttgta aaagccacat ttactaaaat gaatgctgca
     5581 tcaggtgatt cagaatctga aagtatcagt caattttttc atatactagg ctcagtcgag
     5641 caacaaaaag gagtatgcga tactggagaa ggaaaatacg aatatacgat ttattcttct
     5701 tgctgtaacg ttgataaagg gatttattat tatcgtacgt atgaagacag tcaaattact
     5761 gctgtaaata taaataaaga agacttggat cgtcatgaat tggtcagtta tccagtcata
     5821 aaagagcaac agataaacta tgtcaattaa ttaatgaata gatccgtttg gtatcatatt
     5881 ttctgataca gtcataatta gacacgatct ttaggtcgtg tctttttttg ctgttttttt
     5941 tggaaatagc atccactaat atttttttaa cacgaagaga ataaggtagc ctcaaaaaag
     6001 aaaacatggt aaattagata ttgggacttt attttctgta aaaacagaaa ctttcatctt
     6061 ttgttttcac gtgacgaaat tgtaagagag aaaatattcg tccgctattt attctatgat
     6121 aagatgcata tgaatgaaat aaacagtaat tttatattag gatgtgaaag ttaaatgtgc
     6181 ggtattgtag gttttgttaa cgacaaggac aacaaaaaaa caattattaa tacaatgatg
     6241 gatcgaatcg ttcaccgtgg acctaatagt tcaggagaat atatcgataa acatgttgct
     6301 ttaggattca gaagattaag tattattgac ttagagggcg gtactcagcc tatttacaat
     6361 gaagatcgga caaagatcat tatttttaat ggcgaaatct ataattacca gcctttgaga
     6421 gaagaattga ttgcagctgg ccacgttttt caaacacatg cagatacaga agtccttttg
     6481 catggttacg aagaatgggg aacagaatta ttgcaaaaga tccgcggaat gtttgctttt
     6541 gctatttggg ataatgaaaa gaatgagtta ttcggtgcaa gagaccactt tgggatcaaa
     6601 ccatattatt atgcagaaat gaacggaaca tttatgtttg gttcagaaat caaaagtttt
     6661 ctgcctcatc ctgattttaa caaagaattg aacaaagaag cgttgaaacc ttatatgact
     6721 ttccaatatt ctccattaaa cggcgaaaca ttcttcaaag atgtctatcg tttgcctgaa
     6781 ggccattatt atacgtataa agatggaaaa ctagacattc agcaatattg ggatgcagat
     6841 tttgaaacaa aagaaacgca ttcccgacaa gaatggatcg aaaaaatcga cgaaaccgtt
     6901 caagcttcta ttgaagcaca tacggtcagt gatgtagaag ttggttcttt cttatccagc
     6961 ggggttgatt ccagttatgt aacctctgta ttgaaaccag atcattcttt ctcaatcggg
     7021 tttgatgata aaacctataa tgaagcgatt gaagccagaa aactaaccga actattggac
     7081 ttagataata cagcagcagt gatcgacgga gacatgtcgt tcaaagcatt ccctttgatc
     7141 cagtatcact tagatgaacc tgattcaaat ccttcttgcg tacctttgta ctttttagca
     7201 aatcttgctt cacaaagtgt gcgggtagtc caatctggag aaggggcgga tgaactgttt
     7261 gcgggatacc aaacgtatgg attccatacg aattcaaaat tcatccgtgt gattgctcaa
     7321 ggattgaaaa aattaccaaa aggaacacgc tataatttag gacgtaaaat cggaaaaatg
     7381 aaaaatttcc atggacggat ccatttatat gaatcacttg ctcctgcaaa agaatatttt
     7441 atcggtcatg ctcgagtgtt cgaagaatca gaagcatcag aagtgttaac accaaaatat
     7501 caaacagcac catctgtgga tgaaatcatg actgttcatt acgagaaaac agaaggaatc
     7561 aaagatgaag tcaataagat gcaatatgtc gatcttcatc aatggatgcc aaaagatatc
     7621 ttgttaaagg cagataaact ttctatggca agttctttgg aagtccgagt acctttattg
     7681 gatatcgaag tcatgaaatt ggctcaacaa ataccaagta agtttttatt gaatcaaaac
     7741 aacacaaaag atattttcag acaagctgcg aacaaacatc ttccggaaga atggtcgaat
     7801 cgtgtgaaat taggattccc tgtgccaatc aaagcttggt tgaaagaaga acacggctac
     7861 gaacaagtca aagcattatt cgaagcagat tttgcaaaag aattttttga tcaagaaaaa
     7921 atcatgaagt tgctagatga ccatcacgaa ggacgtaaag aagagcaaag aaaaatctgg
     7981 acaatcttca gtttcttgac atggtataaa gtctactttg tagatgaaac gattccgcaa
     8041 gcagaagcaa tcgactatgt gactgtttga tgaaaatcaa atatcaataa aaaagaggtt
     8101 ttctcctacc gtttaacact gagtgataag aaacaatttc aaaaaacagc tcttcccgtt
     8161 ttatagatat tatgacttat ggttcggagt tggcagagga acaccattgt ttcgaacata
     8221 agggggttgt gacacgattt tcgtcacaac ccctttacat atgttcaata atcgatttct
     8281 ttttcttttg tcccatcttt tttataaatg aacaaagttg attctttgtt ttgtgctact
     8341 tgctttcctt tttcgattgc ttcttttttt gtatcaaaat ggttgcttgc cttttttgcc
     8401 cctttagatt tcacgatcca ttgttcttcc tcatattcta cgatcacatc tgaatcaagc
     8461 agttttccag cacgaggatt cgtatcgtgt tcatccgtct tgcttggatt tttttctttt
     8521 tcgaaagttc gtttttctga ttcactagca tcttcatacc atttttttgc ttgatcaatg
     8581 gcaattggga tcgcacggtt atcagggtac ccttcatcta ataatgcatt tgcgatgtcg
     8641 attgcttttt ttcttgtcaa tttatctaag tttttcattg atgcaggaaa gtccttcatg
     8701 ttccatggca tattagtatc cccctttatt aaaaaacata atctatataa acaagtgtaa
     8761 gggtatttat acaaaaagaa aaagattttg tttgagaagt aaaaccgttg tatttatcgt
     8821 caagtagttt tacttaacaa aatcgtgaaa aacactagca atctagaaaa aatcatggta
     8881 agataatcaa gtctgagagt catcttagag gattcttgta aaaatgggag ggaaaaacat
     8941 gcgcgtaaac atcactttag agtgtacttc ttgtaaagaa cgtaactatc taacaagcaa
     9001 aaacaaacgt aacaatcctg atcgtttgga aaaacaaaaa tattgcccac gcgaacgcaa
     9061 agttactttg caccgtgaaa caaaataatg tttttaaagt tctcaattcc ttgctggtca
     9121 aagaattgag acttttttta tctcaaaaca tctaaaatca taaggagcgg ataatatgtt
     9181 tatttccatg tgggcacaag ataaaaatgg tctgatcggc aaaaatggtt tgttaccatg
     9241 gcgattgcca aatgatatgc gtttctttcg cgaacataca atggatcgaa ttttagtgat
     9301 gggaagaaag acctatgaag gaatggggga tctttcactt ccttatcgcc atatcatcgt
     9361 gttgacaacg caaccaaatt ttcgcacgaa agaaaatgcg gaagtaatgc attcgatcga
     9421 tgaacttttg tcgtatagtc aaacgatctc agaagatatc tatgtttcgg gtggtagtcg
     9481 gatttttcaa gaacttcttc cttacactgg tcggatctgg cgaacattga tcgatagcac
     9541 gtttgaaggc gatacgtata ttgggaatat tgatttttca gacttcgccc ttatagagga
     9601 gtatgagggg ataacggatc aagaaaatct atatgcccat cgttttcaaa aatgggaaag
     9661 agtaaaaaat agggttgctt caaaagaaga ataaaatcta tgcgtctagt ttgttaataa
     9721 gaaaaaaagc atctcacgac taatcgtgaa gattcttttt ttagttaaaa tcaatttagt
     9781 ttttctttga tatcattgac catttcctga ctggcaatag gtccgttata tagccaacta
     9841 gattcaggtc cgaattgcag tacatgattg tctgtcactg ctttaagatt cttccagctt
     9901 ggttcattga acatggcagc atctttgtca ctgttcacaa ggatgatgta gtcagcatct
     9961 aattcagcta gcttttcaga ggaaacagca gaccagtcag aagtggcttt tttggagacc
    10021 tcttccacta gatcaggaac accaaattcc agatcgtcgt atacgattct tccacttgac
    10081 cgatttttgc tgaccataaa ggctgagttg ttggtcaccc acaacactgc agctgtcttg
    10141 ttctcgattt tatctgctag ttcttctcga gttttcttga tggatttttg ataatcggaa
    10201 atgatttttt ctgcctcttt ttcttttgcc aaagctttgc cgacatcttt tagttgttct
    10261 tcccatgtga catctgttcc gttttttaca acataagttg gcgcgatttt gctatattgt
    10321 tcgtatttcc cgccttcgac agtagcggaa gaagaaatca acagcaaatc aggctcaaag
    10381 cttaacactt tctcataagg aaggtcataa gaaatcgttg ggacatcctt taaatcatct
    10441 tgcagataat cttgaatgga accactgcca acagtccact gggcgatagg tttttcccct
    10501 agtgcaatca gatagtcttc taagtaactg ccgatgatcc gtttgggcgc gtttggaatc
    10561 tctactttat gtccaagtgt atctgtgact gtatgagtac tattggatga agtcgtcgaa
    10621 ttggcagttg tagctgtttc tttatctgaa gagggggagc aagcgctcag tactccagcc
    10681 agcagcaata tgctagataa tgtgaatact tttgttttta atttcatttc tttcctccta
    10741 acataattga gaatctttct cattggttat tattcctgat atttcgaacg ctgtcaaccg
    10801 cttcatttgg ctatagatat gaaagtagcg cttgacgaaa tgttgaaaca gcgtcataat
    10861 tgagaatgat tttcagcgaa aggatgggtg aaatgacgag attaagagca gctgacattg
    10921 cagccggcta cgaaaacaaa aaaatcattg aaaacatgtc tgttacgatt cctgaaggga
    10981 aaatcactag tttgattggg ccaaatggaa gtggaaaatc tactttattg aaaagtttta
    11041 cccgtatact acctgtgaaa tcaggaaaga tcattgtcaa taactctgat atctctacat
    11101 acagttcaaa agaacttgca aaaaaaatta cattattagc acaaactagt gaacacccta
    11161 caggtatgac agtagaagaa gtagtatctt acggacgata cccttatcaa aaactgttca
    11221 gtggcatgga cacgcaagat catgaagcaa tcgagtgggc gttggatgct acaggtctag
    11281 ctggatttcg tatgcgggat atcgcaacgc tgtctggtgg acaaacccag cgtgtctgga
    11341 tcgctatggc gcttgctcag gaaacagata tcttgatctt ggatgaaccg acaacatttt
    11401 tagatccggc acaccaatta gagatcctac agttattgac tgaaatcaat caaatgaaac
    11461 aaacaacgat cttgatgtcg attcatgaca ttaatcatgc ttcacgtttt tcagattatt
    11521 tattagcaat gaaaaacggt aagttgatca cagaaggtac acctgaagaa gtgatcacaa
    11581 aagagtgtat gaaaaaaata tttgagattg atgcaacgat tgtcacgctt ccaagaacaa
    11641 ataaaccagt ttttctaacc tatgatttgt tgccaaaaga gcaggaggat caattatgag
    11701 gactatgaag aaaaatagac tcctgctttt ggtctgtttt gttcttttga ttattttttt
    11761 tgttgctctt tcaaatggcg cagcgactgt gggaaaagca gatttttggc aggcgttgtt
    11821 tgattttgat agatcaaaac aaagtcacca aatcatacga aatattcgtt tgcctcgtgt
    11881 ggcgggagca tttcttgtag ggagttgttt tgcattgagc ggtgcattga tgcaaggagt
    11941 gacaagaaat cctttagcag attcaggctt actaggaatc aacgcgggtg cttcgttggc
    12001 aatggccctt agttttgctt tttggcctaa agcttctgat ttttctgtgt ttctaatgtc
    12061 attggcaggt tcttttgctg taactttgct cgtatttggt tttttgcagt tttcgaaaat
    12121 gggcatgaat cctttacagc tgattttagc tggcgtagct attagttctt tttttactgc
    12181 tatcagccaa gcacttaccc aattctttaa tctgcagcag gatcttgctt tttggtttgt
    12241 aggcggagct gccaatatca catggaaaca attagcagta ttgtttcctt tatatatact
    12301 tgctgttttg gtttcttttt cattaggaaa agcaatatct ttgattcatt tgagtgaaac
    12361 acatgcatta gctcttggaa gtcatcctaa acgaacgcgg ttgattgttt tctgtctagt
    12421 tgcagtgtta gcagccattg ccgtttcgtt agtcggacct gtttcattta ttgggctgtt
    12481 ggttccgcca gttgttcgcg gaatggttgg aacggactat cgttatgttc tacctgtctc
    12541 ttttttagtt ggcggtggat tggttatgtt ggcggatttt acagcgagga tgatcaatcc
    12601 cccttttgaa acaccttttg gattagtcat tgcattgatt ggcgttccgt ttttactgat
    12661 acaagtaagg agggaacaac ggtgaaaaaa atatattgga cgttctattt cttgcttttt
    12721 gtacttgtca tcctgtctat aggtagttta atgattggta cgccttttat ttacctggat
    12781 cagctttttg ccacgttcat agggaaagaa tcatcttctt tggcgctgat tgttagagaa
    12841 tttcgagttc ctcgattact gatcagttta ttagcaggag cttgtcttgg cgtttctggt
    12901 tatatcttgc aaggagtaac aagaaatgaa ttggcagatt ctagtatatt gggcattaat
    12961 gccggagcag gatttttagt gatgctttat ttaggtttct tttcgcacta ttcgctgccg
    13021 tttctattgc tagttgtagc ctttctaggt ggtgttttag ctgcttgctg tgtctatctt
    13081 gcggcctatg atcgaaatgg ctttttgggt atgaatcgaa tcttattgtc cggtattgcc
    13141 gttaatgccg gcctttctgc attaacattg ctatgcacga tccaattatc taaggaaaat
    13201 tatggatttg tgaatgcatg gttagcggga tcaatctggg gtgcgagctg gccttatgtt
    13261 tgggcattgc ttccatgggc ggttatctta attccgctag gaggacttta tgcacagagg
    13321 attggattgc tatcatttgg tcaagaacgc gcgcaaggat taggccttaa cgtggctaaa
    13381 agtcaaaaaa tccttttgtt gttagctgtt gctttggcaa gcggtagtgt agcggtgaca
    13441 ggtagtttat cttttgtagg actactagct cctcatgctg cgaagctagt cgtacgtaaa
    13501 gaaaataact ggacctttgt tctgagcggt ttgttcggtt ccatttttct gcttagtgca
    13561 gatatggttg ctagagtgat tctaccaagt ggggaagtac ctacaggaat tttgattgcc
    13621 ctttttggtg cgccatattt tctgtatgca ctttttatca gacaaaaaca cgcgctgaac
    13681 agttgaaatt tattaaaaaa ggaaggccaa tcatttctag gtcttctttt tattctattt
    13741 atgcatttat ttgaaagttc gattgctatt tttctttttt tcctatacca ttttatgaga
    13801 gaaggaaggg tgagtttttg gaccaattga aagtaagatt acgccggaaa ggaatcgaaa
    13861 agctgaaaaa tctggccaaa agtggcaaga aaaaggaaaa agaacagcga atctataaac
    13921 agctattcgc cagcgagtac tggaagtcag caaaagtgat agggacgact ttatcgaatg
    13981 aatttgaact ggatacacag ccggttatcc aacgagcaga aaaagaacaa aagaaaatag
    14041 ttgtacctaa aactttgccg aaacgtcaga tggcgtttta tgaaatggat gaacagtccg
    14101 ttcttgtacg ttcgagtttt ggtgttcttg agccacaatc agagaagctc tatttgccgg
    14161 agatgatcga tctcctcatt gtaccaggta ttatctacaa tagagccggt tatcgaatcg
    14221 gttttggcgg tggttattac gaccggtatt taaataattt tcataaaaaa acatgtagcc
    14281 ttgtttttcg tgaacaattg aatgaacaat ggcaaccgga gagctttgat cagaagatcc
    14341 aacaattgtt tattgactaa aatgaaatgg gaggttttca tgaattatca acagcaaata
    14401 aaattgcgtg catggttaag acgaccgtta tggacctatg cttttttagg gatacagaca
    14461 gcggtcttcc tcatgatgga attatttccg cgacttgaga tcccttatta tgccggaatg
    14521 tatggtcctt atcttgttca cttcaatgaa tggtggcgtt tagtgacacc aatcttcatc
    14581 cattttggac tgatgcattt cgtaatgaac tcactgatac tgtattttat ggggcagcaa
    14641 atcgaggcaa tctatggcca ttggcgtttt ttcttgattt atatgttcag cgggataatg
    14701 ggaaatacag caagctttgc ttttaacgaa gcgaatgttc tgtctggtgg tgcgagtact
    14761 tcgatattcg gtttgttcgg tgcgttgttt atcttagggt ttcattttaa acataaccca
    14821 gcgatccagc agctagtcag acattttctt ttatttattg tgttgacatt tgtttttggt
    14881 cttctggata cctctgtaga tgtttgggga catgtcggtg ggctaattgg cggattagtg
    14941 ttagggaatg ttttgggtct accgaaacaa cagacaagct attcgataca ccaaaggatt
    15001 ctttctacac tagtttttgt atttcttttt gtaatatgta ttttactggg tttaaaaaaa
    15061 tatggtttac ttgtataata tgaaggaagt gcttaaagat ggagagctta tatgatgtac
    15121 agcagctgtt caaaaaattt ggcatcttta tctatgtggg tgcacgcatc tatgacattg
    15181 aattaatgac tatcgaattg cgtaatctgt atgatagccg tttgattgat catgatacat
    15241 acatgacagc gtggcgcata ttaaaaagag agcaccgaat tgaagaaagc cgaaagaaag
    15301 gaaatttgta atggacaaga agattattgg gattgattta ggtggaacaa cagcaaaatt
    15361 tgcaatttta acaccagaag gagagattca acaaaaatgg agcatcgata ccaatatttt
    15421 ggatgatggt aagcatatcg ttcctgaaat cattgaatca atcaaccatc gtttgaattt
    15481 atataatatg aaagccgaag actttatcgg aatcggcatg ggtaccccag gaagcgtgga
    15541 cagcgaagct ggaacggtta ttggtgcgta taacttaaac tggacagaag ttcaatttgt
    15601 gaaaaaactg atcgaggcag gaacaggaat caaattcgct atcgataatg atgcgaatgt
    15661 tgctgcttta ggtgaacgat ggaaaggtgc tggagaaaac gatccagacg ttgtattcgt
    15721 tactctagga acaggtgttg gcggtggtat cgttgctggc ggaaatttga ttcacggcgt
    15781 gagcggagca ggcggagaga tcggccatat cactgtggat cctgaagggt tcgaatgtac
    15841 ttgcggtaaa cgcggatgtt tagaaactgt gtctagcgct actggtgtgg tacgtctagg
    15901 tcgttacttg gcagaagaat acgcaggaga ttcaaaactg aaagcaatgt tagacaacgg
    15961 tgaagaagta acaagtaaag atatctttga aatggctcaa gaagacgatc cgtttgcatt
    16021 gatggttgtt gatcgcgtat gcttctactt aggtttagct tgcgccaact tggggaatac
    16081 attgaatccg tcaagtattg tgttaggtgg aggagtatcg gcagcaggag aatttttacg
    16141 tagccgtgta gagaaatatt ttaaagaatt tactttcccg caagtaagag aatcaacaaa
    16201 aatcaaatta gcagagcttg gtaatgaagc tggtgtcatt ggtgctgcct cattagcctt
    16261 gaaatttgct taaaaaaacg gaggcattac atggttttat gggtaattaa tatcattttg
    16321 ctattgattg tgcttgcaat catcttttgg gaagtctatc tacgagtaat ggccaagcgt
    16381 tcagcaacga ctttgacaga agaagaattt cgagaaggaa tgagaaaagc acaagtaatc
    16441 gatgttcgcg aaaaagatgt gtttgatgct gggcatattt taggtgcaag aaacatccct
    16501 tacactgttt tgaaagattc attaggatct attcgaaaag accagcctgt ctatatttac
    16561 gatcaaaaga aaagcttgag catccgtaca gcaaataaat tacgtaaagc aggttatcaa
    16621 gagatttatt tacttaaggg tggatatgac ggatggtctg gaaaaatcaa aagtaaaaaa
    16681 gtataaaaca gatgattgta tgacagcttt aaaaaccgaa tcaatcatta gattttgacc
    16741 aagatcacat gaacggcgca atggatagtt gtgccgttca ttttttttat tttgtgctat
    16801 tatctacata tataagctaa gcgaaaaggc agaaagaagg aaacaactat gatcaaatta
    16861 aaacgtgttt accttgatgc tgaaaaagag gatggctata gaatactcgt ggataggttc
    16921 tggccacgag gaatttcaaa agaaaaagcg cagttagatc tgtggttgaa agaaattgca
    16981 cctagtaatg aagcacgcaa aacttttgac catgtaccag aacgatttgc ttcatttgca
    17041 aagaaatacc agcaagaatt gattttcgaa gaagaaaaga agcaagctat ttttaaactc
    17101 agtcaactta tgaaagaaca tccagtcatt acttttgttt atggtgcaaa aaatgaacat
    17161 cagaaccatg ctgttttatt gaaaaaattt atcgaaatgg aggcatgcaa atgaaaatag
    17221 ggatcattgg tgccggacct agaggattaa gtatagcaga aaggattctc cataataatc
    17281 aggacagaca ttctcttgaa ctttttcttt tcgatcccaa tggtcctgga ggtcgagttt
    17341 ggcggttgaa tcagccaccg gagttattaa tgaattctgt ttctcaacaa gtgacgcttt
    17401 ttacagatga aacattgtcc agtggaggat tggtaacaca gggacccaac ttgtttcaat
    17461 ggagtaaagg acctgctaca gattatatcg aacagacggt aaccaaaaat aagcaactaa
    17521 ttatagaaga aatcaatcgt ctggaaaaag atcagcaatc tactcgatgt ttatacggac
    17581 tctatcaacg ctggtttttc tcaaagttgc aagaagaatt tccagcttct attcagtttg
    17641 tttattcttt agtaaaaaaa gtcagcaaaa gaaaagatgg ttatctttta cagacagatc
    17701 agcaaagcta ttctatcgat cagcttgttt tagcaagcgg tcactgggaa aatgatgaga
    17761 cagaagaaga aagagtgtgg gtagactacg caaagaaaaa tgatttactg tatcagcttc
    17821 cttctaatcc tgcagatgtc gatctttcga agataccgcc taaagaaaat gtctttttaa
    17881 gaggtctagg cttaagtttt tttgattatg tcggattgtt tacagtatca agagaagggc
    17941 gcttcgagaa cgaaaaagga aaacttgtct atcatccatc aggcaaggaa ccgattgtat
    18001 acagtggatc aagaaggggg ctcccttatt accctcgagg aagaaaccaa aagcaaggcg
    18061 gatcaatggc atggccacgg ttactaacaa aagaaaacct ggagcaatgg catcagtcgg
    18121 gatttttgac tggcgaaaca ttttttgact atctgaaaaa agacgttgag ttgttttatt
    18181 acaagaaaat catcgaagaa tatcatttgc cgattccaaa gaaaaatttt gagcatgatt
    18241 ttctctccct ctcttcagag gagtgtctcg gaaagcatcc agcactaatc ccttatagat
    18301 gggattggtc gtatttagaa attcctctta cctatcagga tgagtccttt gcagaagcta
    18361 gccgagcatt tattgaaggg cagataaaag aagcggaaaa aggcaattgc acaggagcat
    18421 ttgcttcaac atttgatgca ttaaaagact ggcgtgatcc aatccggcag gctattgaat
    18481 ggcacgtttt ttctgcaaag gaatacaaag aaatactatg gggatggttt actcatctaa
    18541 atagcttttt aacgatcggt ccgccagtca ttcggacgaa agaattagca gcactgatag
    18601 atgcgggtat ttttcatttg ttcgaaccgc catttgatat tcagcaaaag cacaaccgct
    18661 atgttatttc tctacaagat aaagaaatca gcagtcgcta tttgattgaa gctcgattgc
    18721 cacaaacaag cttttctcgt tcaagtaatc ctgtcataaa aaatttgaag gaaaacaaac
    18781 ttgttcgccc tttttcattt tcagataata caggaagtta tcattcagaa gctatagatg
    18841 ttgagcgagg tacgaatcgc gtgattgatg cttcagggga aatccagtct gacttttatt
    18901 gctatggaat acctgttgaa ggtgtggatt ggctaactgc agcagtagct agaccttata
    18961 cagatccatg gaatctgaga caggcagata aaatagcaca acttatattg actgacaaat
    19021 aaaaatacgc atgatgactt atctgttctt tcacaagtct tcatgcgtgt ttttaaataa
    19081 ttattaacga gaaacgcgtt tgcgcattgc ttttttacgg ttttcttcga tcattgcttc
    19141 tttttcgtca attggatcga tcactgtttt tttcacagaa gcgaccaaac cagcaacggc
    19201 agcggctgtt gccagacttc ctactaatat tccagataca aattttttca ttgaatccac
    19261 ctcatttatt atttgttata tctattatgg aacaaaagag aaaaaaaggc tagttttacg
    19321 tttgtttgta aacctttgca aaaaatcctt gaaaatgttt actaaatgtt ttattataaa
    19381 tttaccatat gaatttacga aattttactt tgtttgtttt caccataaaa gtaaacttcg
    19441 tttttttaaa ttatatataa tcaacaaaca caattttact aatcaaagga gtgtttttta
    19501 tgacaatttt agtactaggc ggagccggtt atatcggttc gcacgctgta gaccagctag
    19561 ttcaaaaggg gtatcaagta gctgtggtag ataatttgct cacaggtcac aaacaagcag
    19621 tacatccaga tgctcatttt tatgaaggag acatccggga taaagaattt ctacgttccg
    19681 tatttgaaaa ggaaccgatt gaaggagtaa tccattttgc tgcaagctct ttagtaggag
    19741 aatctgtcga aaaaccattg atgtatttca acaacaacgt atacggtatg caaatcttac
    19801 ttgaagtcat gcacgagttc aatgtgaata aaatcgtgtt ctcttctact gcagcaactt
    19861 acggagaacc aaaagagtct ccaatcacag aagatacacc agctaatcct aaaaatccgt
    19921 atggtgaaag taaattgatg atggaaaaaa tgatgaagtg gtgtgaccaa gcatatggca
    19981 tgcgctatgt cgctttgcgc tacttcaatg tagccggagc aaaagcagat gcatctatcg
    20041 gagaagatca tacaccagaa acgcatcttg tcccaatcat tttacaagta gctcttggtc
    20101 aaagaaaagc attggctgtt tatggagacg attatgatac accagatgga acatgtatca
    20161 gagattatgt tcaagtagaa gatttgattg ctgcacatat tttagctttg gaatacttga
    20221 aagaaggaaa tgaaagcaac ttcttcaatt taggcagcag caaaggatac tcagtcaagg
    20281 aaatgctaga agcagctaga gaagtgacag gaaaagaaat tccagcagaa atagcaccaa
    20341 gaagagctgg agatcctagc cgattagtcg cttcaagtga aaaagcgaga gaggttctgg
    20401 gttggaaacc tgaatacaca gatatcaaag caattatcaa aacagcatgg gattggcatg
    20461 tcagccatcc aaatggttat gaagaatagg ggaatgagat atgtcaatca gtcaaacgat
    20521 ttgtgatttt gccacacttg cgatcgaagc tggcggatgg atggaaatgg accgcttata
    20581 tttacagaat cgtcttttag ggatggtagg agaagatgct ctagaaagca ttgaagtaag
    20641 accagttaag gaagaatctg tagacttatt ggatcagctt gtcgctaagg caagagaaaa
    20701 tggtgttctt tcagaattgc cagcagaaca agagattttt gaagcgcaat taatggattt
    20761 tctcacaccg ccaccttcag tcgtcaatgc attctttgca cagcactatt cgaaaagccc
    20821 agaggaagca actgagtatt tctttaattt aagtaagaaa aatgactaca tcaaaacaag
    20881 agccatcgca aaaaacatca aatttccggc agagtcggag tatggtgaac tggaaatcac
    20941 aatcaattta tctaagccag aaaaagatcc aaaacaaatc gcagcagaac gcgaggcggt
    21001 aaaagtagac tatcctacct gcatgctttg catggaaaac gaaggatata gaggtcgctt
    21061 gaactatcca gcccgcacca atcaccggat tattcgaatg aatctagacg gtgaaagctg
    21121 gggattccaa tattctcctt atgcttatta caatgaacat tcgatcattt tatcagaaca
    21181 acaccgcccg atgaaaatca caaaggaaac atttgctaga ctgcttcgga ttactgaagt
    21241 cttgcctcat tactttgttg gttcgaatgc tgacttgccg attgttggag gatcgattct
    21301 ttctcatgat cattatcagg caggacgtca tgaatttcct atggcaaaag caccgataga
    21361 aaaacttatc aaactagcag attatccgca agtcatcgca ggagtcgtga aatggccgat
    21421 gtctgtgatc cgtttgcaat caacagagaa aaaattgttg attgaagcag ctaactcagt
    21481 attggaaaag tggaaagcat attcggatga aacagtatct atcagagctt tttcagcaga
    21541 tggtacacca caccatacga ttacgccaat tgcacgaaga agggacaatt actttgagtt
    21601 ggaccttgtc cttcgagaca acaatgtttc agaagaacat cctgatggga tttttcatcc
    21661 acataaagat gtccaacata tcaaaaagga gaacattggg ttgatcgaag taatggggct
    21721 tgctgtttta ccacctcgtt tgaaaccaga actagtagaa gtagagcatt tcttattagg
    21781 agaagaaaac cagatggctg aatatcatcg tccgtgggca gaacaattaa aaaaagaaca
    21841 tcctgatttg acgaaagata cggtcaccga tgtcgtacaa aaatctgtag gtcaggtttt
    21901 tgcacgtgta ctggaagacg ctggagtatt caagcgagat gaaaaaggac aggcagcgtt
    21961 tgaacgattt actgcaattt tataaacgta aaaaaggagg agagacaaat ggcaacaatt
    22021 aaagacattg cgcagttggc aggagtttct cctgctactg tttcaagagt tctcaactat
    22081 gatcctgatc tttccgttgg cttagccaca aagcaaaaaa tttttgaagc agcagaacgc
    22141 ttaaattata cgaaacatcg taaaaatgca gcaaatacta agcaaacgct tcgcctgatc
    22201 cagtggtacg acgatacgga agagcttgaa gatctttatt atttatctat tcgtctagga
    22261 atcgaaaaaa aggcagaaga agcaaacgtt actttattga aagaaacgtg ggattccatt
    22321 agtgatcttc cgacagatgg aacgatcgca ctaggaaaat tcgacgaagc tcagttatta
    22381 gaaatgaaag gatcccaaaa tgccttgctt ttggtagatt ctgacggcac ttctcaagga
    22441 atcgactctc tagttgtcga ctttaagtca agtgttcaaa aagtcattga acattttgtc
    22501 tcccataaag cgcaacatat cgctatgctt gctggaacag aatatacgaa gaaaaaccat
    22561 cagcggctaa aagatcccag aattatggca tttcaacaag ttttggcaga aaaaggctac
    22621 gacgaaggtc caattatcga agcagatttt tctgtcgagt caggatatca agcagtgaag
    22681 cagtatttaa aaacgaatcc gcatgttcct gatgcgcttt ttgcagctaa tgatgcatta
    22741 gcgattggcg cactgaaagc gattcaagaa aaaggtttag tggtaccaga agatatttct
    22801 gtgatgggtt tcaatgatgt cagtgtagcg aaatatgtaa cgccctctct ttctactgta
    22861 aaagtttata ccgaatggat gggagagcta gcagtggaga cgattcttca attaagtaat
    22921 tccaaagcac cggtttcaag aaagattatt gtggaaacag agttgattat tcgagaatca
    22981 acgaaataaa agatggaaaa gctgtgggga aatgaagtga taccgcagct tttttgtgtt
    23041 tcaggaaggg gcaaaatttg cccgccagca gataatcatg tatcattgta ctagttttgc
    23101 ctttggtatt ttcatcctca gaataaaaaa gtatgctaca atggaaggcg agaatgtcat
    23161 agcagaaagg atcaaatata tgattacatt aggaatagat actgcaaacc aaactttggc
    23221 gatcggtgtg gtagaagacg aacaaattct tggacaaata caaacaaata tcaaacgcaa
    23281 ccacagtgta acactgatgc ctgcaatcga ccaattgttt gcagatcttc aaataagtcc
    23341 aaaagacatt gaccgaatcg cggtttctga tggtcccggt tcttatacag ggcttcgtat
    23401 aggtgtaacg acagcaaaaa cgattgctta tacattagat aaagagctag taggagtatc
    23461 tagtctgaaa accatcgcgg ctaattgcat cggtgtggat aaaattattg tgcccatgtt
    23521 tgatgctcga agaaaaaatg tatacgctgg ggcataccgc tggaaagaaa atagattaga
    23581 aattgtactt gaagatgtac atatttctgc ttctgatttg ttcacacaac tacaaaagct
    23641 gaatgaagct atttatttcg tcgggagtga ttgtcacaaa ttcttctatg aaataaagga
    23701 aatattgcct agtgcgatga ttaatacagt tccagcatgg gatattccaa gtggtgtaac
    23761 tgtggctcaa ttaggtgctt tagctgaacc agttgcagag aaacaagctt ttcttcctcg
    23821 ctatttaaaa cgggtagaag cagaagaaaa atggttggag acgcacacac ctggagacga
    23881 aagttatgtt gaaaaaattt agagcgtatg ccagaagctt ttttcaagat aaaatgattt
    23941 atacagaaaa aacaatcgaa caaatcgatc gtcgttacgt actaagagaa ttgactacag
    24001 aagatatcaa agagctgctg gatgtcgaac gagaagtcta taatggcgaa ttgccatgga
    24061 caaaaagtgc ctttatatca gaattgaagt caccgcttcc tcatctatat ctaggtatct
    24121 ttgataatgg aagaatgatt ggttttatcg gttcaagagc gttcggttta gattgccata
    24181 tcacgaatat tgccgttcga ccggcatatc agcgtaaacg aatcggttcg ctgcttattg
    24241 acgaaatcga gaactttgct ataatgaatc gctgtgagac gatgtcatta gaagtgcgta
    24301 tgagtaatca agatgcacaa agactttatc ggaagttagg ttttcaagca acaacgatca
    24361 aaaaaagata ttacactgaa acaaaagaag atgcgttgga tatgataaaa cttttagaaa
    24421 atgagtgaga ttagttgatt tgcagaaaaa aagattttga tgaagcgtac ttggcagaat
    24481 gcgtatggat gattagcgaa aatgcttatc cacatggttc accttggacg aaaatgcagt
    24541 ttttggcaga catccaacag cctcactctg attatctgct tttagttgaa gataaccaga
    24601 tctgtggctt tattggatac tcgaaagtaa tagatgaagt cgagatcaca aatattgctg
    24661 ttgcagtttc agaacaagga aaagggcatg cacggcattt gttacaatta ctgattacgg
    24721 aacaaataaa agattcgctg catgtttttt tagaagtacg tctatctaat gaagcagcaa
    24781 gaaaacttta tgaatcagaa aagtttcgta tattaggtaa acggaaatca tattatcgca
    24841 atccgacgga agatgcgatc attatgagca gaaagttaaa aacggagacg atgaaatgaa
    24901 tgaagaattg attctggcga ttgaatcaag ttgtgatgaa acgagtgtag cggtcgttag
    24961 aaatggaaca gagatcttat ctaacatcgt tgcttctcaa ataaacagtc ataaacgttt
    25021 tggtggcgta gttcctgaag tagctagccg ccatcacgta gaacaaatca ccttatgctt
    25081 ggaagatgca ctggtagaag ctggtgtgtc ggcagaagat ttatcggcag tcgctgtcac
    25141 ttatggacca ggacttgtcg gctcgttgct gattggtatt tctgcagcta aggcttttgc
    25201 ttgggcacac caattgccgc ttattcctgt aaaccatatg gcagggcata tctatgcagc
    25261 ccgtctggtt aaaccattcc agtttccatt gatggcactt cttgttagcg gaggacatac
    25321 agaattagtc tatatgcaag aagatggatc atatgaaatc attggggaga caagagatga
    25381 cgcagcagga gaagcttatg acaaagttgg aagagtatta ggtctttctt acccaagcgg
    25441 aaaagaaatc gatcagctag ctcatcaggg aaaagataat taccattttc cacgagcgat
    25501 gatccatgaa gataattatg atttcagttt tagcggatta aaaagtgcat ttatcaattt
    25561 ggtgcataat gcacaacagc gtggagaaga tctagacaaa aatgatttag cagctagctt
    25621 tcaagcaagt gtgatcgatg tattgattac taaaacactt cgagcttgtc aaaattatcc
    25681 agtcaagcaa ctgattgtag ctggaggtgt agcggccaat caagggttgc gtgaaggatt
    25741 gcaggatgct ttggcaacta aactccctga ggtagaacta gtgattcccc ctcttcgact
    25801 ttgtggagat aatgctgcaa tgattggagc agcagcacat gtggaaatgc aaaaaagaaa
    25861 ttttgctagc tatcagttga atgctgatcc aagcttggtt ttatctgaat aaagaggttg
    25921 tgaaaaatct gccctgcatc aagtaataag aacagcccag gaaaaacggc ttctcatgtt
    25981 tttgacctgg ctgttcttat taccgcgtat gcaagctttt gaacgccgtt taatcaagag
    26041 ttcgtaagcc tgtcgtttaa ctctgattca caaggaacaa tttcaaaaaa cagctcctca
    26101 tgtttatgaa cattatgact tgtgaccgaa gagttgacag gtacacaccg tttaatcgaa
    26161 ataagggctg tgacacgctt ttgtcaaagc cctatttttt acagcttatt catcattatc
    26221 cgttcctctg attgccctca ctgattaatc gtttattgtc gaggaatatc gaataatcta
    26281 ggtcggatga ggacaagacg cttcctctag ttaatctatc agtatttttt gcaaacagtg
    26341 aatattacat aactgaaaca cacatagaaa atctttgagt cttgcccagt gatctaaaac
    26401 tgccggatct ttcagaagtt ttagggagga aaataagcag tgaccctgat aaatatcagg
    26461 gtcactgctt atttttgttc gcgttattct ctcatagctg gagtagtagc tgtcttacag
    26521 aaagtaaaac gctttttgtc ataatctaac agtccggaat cacgtagttg tttgattact
    26581 tgtccagcag tttcacgtgt cgtgccgcta ttcactgcta attcttttaa ggtgatcgga
    26641 taaggaatgg tgatatgacc atcttttgtt ggtacgccca attcttcttt caaaatgaat
    26701 aaattttgtt ttacccggtc aaaggcgcta gaggtcaatc cgatttggat acgcaattca
    26761 tgtatttcaa ctactttaga aattttttgt atcatataga tcaactgttc gggatttttt
    26821 cgtgctaatt tttcatagta ggaaacagga aggtagaatg tttcaatatc agtcatcgct
    26881 tgagctgtat aagtgtaggt ctcttctcca aacattcctg acataggaaa aaggttattt
    26941 gtattcacat agtcatagta gtaataagtt gcactttcat cataacgttc taaacgaacg
    27001 agtcctttgg tcaagatata caaccgttgt ctgggatcac catcgtcgaa aaggatctgt
    27061 ccttttttat aagaacgaag aacagtatgt tccttgatct gttggaactc ttcatccgag
    27121 aaatgttcaa aatcaggttg atatctgagt tttgaaaagt gaaaatcgat tttattaaca
    27181 cgcattaaaa agtcctctcc ttattgttca ctatatttat tgtcactttt tttaaagaat
    27241 ttgtaaagtt ttaacatacc aacattagaa acaagtttta atactttaca aatataattt
    27301 gtt
//
LOCUS       DACDRN010000033        26872 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDRN010000033 DACDRN010000000
VERSION     DACDRN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.033"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1086)
                     /locus_tag="IWJ80_001631"
     CDS             complement(<1..1086)
                     /locus_tag="IWJ80_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9461347.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKE"
     gene            1296..2297
                     /gene="ccpA"
                     /locus_tag="IWJ80_001632"
     CDS             1296..2297
                     /gene="ccpA"
                     /locus_tag="IWJ80_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP9461348.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(2634..4979)
                     /locus_tag="IWJ80_001633"
     CDS             complement(2634..4979)
                     /locus_tag="IWJ80_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9461349.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            5258..6160
                     /locus_tag="IWJ80_001634"
     CDS             5258..6160
                     /locus_tag="IWJ80_001634"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP9461350.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            6373..8148
                     /locus_tag="IWJ80_001635"
     CDS             6373..8148
                     /locus_tag="IWJ80_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP9461351.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(8214..9800)
                     /locus_tag="IWJ80_001636"
     CDS             complement(8214..9800)
                     /locus_tag="IWJ80_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP9461352.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            10051..10275
                     /locus_tag="IWJ80_001637"
     CDS             10051..10275
                     /locus_tag="IWJ80_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP9461353.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            10417..12168
                     /locus_tag="IWJ80_001638"
     CDS             10417..12168
                     /locus_tag="IWJ80_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9461354.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            12168..13952
                     /locus_tag="IWJ80_001639"
     CDS             12168..13952
                     /locus_tag="IWJ80_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9461355.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            14278..15129
                     /locus_tag="IWJ80_001640"
     CDS             14278..15129
                     /locus_tag="IWJ80_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9461356.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            15183..16934
                     /locus_tag="IWJ80_001641"
     CDS             15183..16934
                     /locus_tag="IWJ80_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9461357.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            16938..17108
                     /locus_tag="IWJ80_001642"
     CDS             16938..17108
                     /locus_tag="IWJ80_001642"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461358.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(17148..18050)
                     /locus_tag="IWJ80_001643"
     CDS             complement(17148..18050)
                     /locus_tag="IWJ80_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9461359.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            18281..19684
                     /locus_tag="IWJ80_001644"
     CDS             18281..19684
                     /locus_tag="IWJ80_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9461360.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            19684..21117
                     /gene="ascB"
                     /locus_tag="IWJ80_001645"
     CDS             19684..21117
                     /gene="ascB"
                     /locus_tag="IWJ80_001645"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9461361.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            21262..22188
                     /gene="trxB"
                     /locus_tag="IWJ80_001646"
     CDS             21262..22188
                     /gene="trxB"
                     /locus_tag="IWJ80_001646"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP9461362.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            22402..24129
                     /locus_tag="IWJ80_001647"
     CDS             22402..24129
                     /locus_tag="IWJ80_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP9461363.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            24201..24494
                     /locus_tag="IWJ80_001648"
     CDS             24201..24494
                     /locus_tag="IWJ80_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9461364.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(24586..25446)
                     /locus_tag="IWJ80_001649"
     CDS             complement(24586..25446)
                     /locus_tag="IWJ80_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP9461365.1"
                     /translation="MTSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            25538..25966
                     /locus_tag="IWJ80_001650"
     CDS             25538..25966
                     /locus_tag="IWJ80_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9461366.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            26128..26304
                     /locus_tag="IWJ80_001651"
     CDS             26128..26304
                     /locus_tag="IWJ80_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP9461367.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            26331..26777
                     /locus_tag="IWJ80_001652"
     CDS             26331..26777
                     /locus_tag="IWJ80_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP9461368.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         8552 a         4787 c         5681 g         7852 t
ORIGIN      
        1 ttcttttggt gtagtcgtga atggcacaca gccatcgctt gtcacatata cacagtcttc
       61 gatacgtacc cctacttttt caggaatata gattcctggt tcaagagaga agcacatccc
      121 ttcttcgatt actaagtcat ttccttctac aagggaaggg aattcgtgga cggttgttcc
      181 gatcccgtga cctaaacggt gattgaaata ttcgccgtag ccatagctgt tgatcacgtt
      241 tcgtgcgatt tgatcaagtt cacctgctgt cacacctgga cggacggctt cttgtgcggc
      301 aagctgtgct tcaagagtga tgttgtagat tttttcttgg aagtcggttg gtttttgata
      361 agcaattgta cgagtagcat cactgcaata gccattccaa acaacgccaa gatcgaataa
      421 cacaagttca ttcggtgaaa tcttcgtatt tcctggtgta ccatgagggc ttgcagcatt
      481 tgctcctgca aggactaatg tgtcgaaaga catttgtgaa acaccttgtt ttttcaattg
      541 gtattcaatt tctgcaatga tcgcttgttc agtcacgcct tctttcactg ctttaaagcc
      601 aatctcaaat gcaacatcgg cccatttacc agcttcgatc agtttgtctt tttcctctag
      661 agttttgatc agttgtagac gttggatgat ggaagttaga tcagtgtaag aatccgttcc
      721 cgtcaagata tcagagatag catcgaagcg ctcgacagtc aaggcgttct tttcaatccc
      781 tacattgcca ggctgaccat agcgagcacg aagttctttt ccgatgatct cccaagggtt
      841 ttcgctgtct aaatagccac ggacatcata cgcccaacta ctgttttgag catcttcaac
      901 ttccaaagca ggagtgaaaa ggaaaggatc ttgttcaaga gggataaaaa gagctagtac
      961 acgctcatga ggatcacttt cgtatccgga aaagtaagcg atgtggccag ggtcgctgat
     1021 ataagccaat tcgacttggt gttgtgccat ccattgacgt agttcattga tttttacgtt
     1081 attcatttgg caacctactt tctaaattaa ttttgtattc aataattgta ccactgattt
     1141 attaaaagac caacgttcta aaaactgaaa aaagtaaaaa ttttcatgaa aacagagata
     1201 aaacggttgc aattcttgag attatttgat attctatttt agaaatgtaa gacgtatttt
     1261 cagaaaacat ataaaatatg aataggagaa cacaaatgga aaagcaaaca atcactatct
     1321 atgatgtagc gagagaggca aatgtttcga tggctactgt ttcacgtgtc gtaaatggca
     1381 atccaaacgt taaaccagca acaagaaaaa aagtcttgga agttatcgat cgcttggatt
     1441 atcgtccaaa cgcagttgcg cgtggcttgg caagtaagaa aacaacaaca gttggtgtaa
     1501 tcatccctga tgtcagcaat atgttctttg cttctttagc acgtggtatc gatgatgtgg
     1561 caacaatgta caaatacaat attatcttag caaattcaga tggcaacgac caaaaagaag
     1621 tcaatgtatt gaataactta ctggctaagc aagtggacgg cgtgatcttc atgggtcacc
     1681 atatcacaga cgaaatccgt ggagaatttt cacgttcaaa aactcctgtt gtattagctg
     1741 gctctattga tccagatgaa caagtaggta gcgtaaacat tgattatgta tccgcaacaa
     1801 aagatgcagt aaacaaattg gcaaaaagcg gcaataaaaa aatcgctttt gtcagtggtg
     1861 cgttgatcga tcccatcaat ggacaaaatc gtctgaaagg ctataaagaa gcattaagcg
     1921 aaaataacct accatatagc gaaggattga tctttgaagc acaatacaac ttcaaagatg
     1981 gtctatctct agtcgatcgg atccataata gcggtgcaac agctgtctat gtttcagatg
     2041 atgaattagc catcggtatt ttggatggtt tattagaccg tggcgtgaaa gtaccggaag
     2101 aattcgaaat catcacaagt aacaattcac tactgacaga agtagctcgt cctcgtttaa
     2161 caagtatcac acaaccatta tacgatttag gggctgtatc tatgcgccta ttgacgaaaa
     2221 tgatgaataa agaagaagtc gaagaaaaaa cgattatctt gccttatgga atcgaagaaa
     2281 aaggatctac aaaataataa aaggacaacc attcagaggt tgtgtgcctg ccgtttaact
     2341 ctgcgttaca aggagcaatt tcaaaaaaca gcatctcatg tttgatgaat attgtgactc
     2401 gtgattcgga gttggcagga gaacaccgtt tatttgggaa aaggggttat gacaaaattt
     2461 tgttataatc ctttttttgt gtcttaaaaa agagagaaag tggttgaaat tacatttatc
     2521 gtatataaaa caatccgtgt atagatgctg cattttgttc accaccatca aaaaaataag
     2581 ggactgtgac aatactattg tcacaatccc ttattactgt gtattctatt aaatcaatca
     2641 tctttctcat ctgattcttt agttgcttga ttcgctcttg cataagctga agcggtgttc
     2701 cagtaatctg tatagttttc atcggtaccg ccaacgccaa atttaaaggc gcttttcttc
     2761 ggtccatttt tcgcccatag cgattcaact gtttttcctg gtgtttggat ggatttttta
     2821 ttgacagtaa cttttccagg tgtttgacca gtaaatccag atactttttt cttcatcaca
     2881 tcagatgata attcgaattt ctcatcagtg ccaaaaatgt caggttttgc ttggtaagct
     2941 tgattgatca gattggcaag ataattagcc gaacgtatcc ttgctttgct gtccattcca
     3001 gtattgtcat cgtgccctgc ccagctactg atggtaatag acggagtaga gacaattagc
     3061 catgaatctc ggtattcatt ggtagaacca gtttttccga cccaatctgc ttttcctaga
     3121 tttccgttta agcttgagat ggcatctttg aatggtgtag tgatttttgc attgatcaca
     3181 ctacgcatca tgtcattcat gatcgatgcc gttgcttccg aatagatccg gacagagttg
     3241 ctttcgtgtt tataaattac atttcctgcg ttatctgtga tggaatcgat gatatagcct
     3301 tcttcataga cgccgccgtt tgctaaagcc tgaaatccgt tggtctgttg aagcgtcgtg
     3361 acatctactg tacctaaagg agcagattca acgccccaat tgtcatttgc cgggtagttc
     3421 atctttgcca agtagtgctc gtaagcatat tgtttcgatc caccggaatt caacacatct
     3481 tgatataagt gataagccgg gatattgttg gaccattcta gtgactcacg gacggtttgg
     3541 aaggtgttcg atccttcgtt agtagcattg acgattttct cgccagcatt ttctccttct
     3601 tgccaagttg ttgcataatc tgatacacgg gattctgatc cgataagccc catgtcgatt
     3661 gcaggtccat aaacaagaac tggtttgatt gatgagccag cttgacgttc tgtatcgaag
     3721 gcatggttat tttgattctg actgtaattc cgtccgccaa cgaatccgta gatgcggcca
     3781 gttctgttat ccatcaatac atttccggtt tctacttgac tgccagcacc atcatctaac
     3841 atgtatccat aattttcaac gccattttgc aatgcagcat agacatcttt atcgattgtc
     3901 gaatggattg tatatccttt attttggatc gtttgctgtg ccttttgata ataagcgtca
     3961 ctgaccgatg aatcagacat atcagcgttg tctttttcag ccaattgttg tgcaatgatt
     4021 cttgttgctt cattcatcac agtataatac aagaattctc tgtcagtctg ttctgcgatt
     4081 tgttgtggta gaaagtcttt tgtaaggtca taggcttttg cttcttcgta ttgttctttc
     4141 gtaatttggc cttcacgata catactgaat aaaacaaaat cttttctggc aaggccggca
     4201 gaaagatctt cttttagcgc gcctgtgttc gtgtatggac tatacgtgat cgggctttgg
     4261 ggtaatccag cgatataagc agcttgcggc agcgtcacat cttttgcatc tacaccaaag
     4321 attccttttg cagcttcctg gactccagca atgttttgtc ccttattatt acgaccaaat
     4381 ggtgagacat tcaaatacgt agcaatgatc tcatcttttg aaaagtattt ttctacttgt
     4441 gcagaaagaa tgatctcgtt tgctttacgt ttgaatgttg tttcatctgt aaggatctgc
     4501 tgtttgacta attgctgtgt caaagttgat ccaccagaag agccaatacc agtagcttca
     4561 gatactaatg ctcgtaaaac agctttagga acgtaacctt ggtgtttatc gaagtattca
     4621 tcctctgtgc tgataattgc agttttcaat aaaggtgaaa tcttatcaga tgaaatagtc
     4681 gtgcgcatca gatctgtctg gatttttgat atctctgtat tatctgcata aacaatcttt
     4741 gacgtttctg taatgtttcc tagttctgtc tgcagtgctt ttttagtcgg caattgtgtg
     4801 tcttcgacga gataggcaaa atagccagcg cctattccaa tacctagagc tgcgaacatc
     4861 aaaagaagaa cagtaataaa aacagtcaat gattgaaaaa ctctaaaaat aatcttagga
     4921 acaaaccatt tttctttttt tcttctattc tgtttttttc gagtattttt gcgtggcaaa
     4981 agggtcacct caactaatgg ttttatttcg ctaattatag caaataatag aacaaaaaaa
     5041 cagacaaggc tgtttttggc aggaatttct tttttaaaag agttaaaacg tgagaaaatg
     5101 atctttatta taaaagagtt cttaagcttt tctttagaga aaatttcagt tactatggag
     5161 aacagggcaa agaaaaatca atcattttct ttgtatatca tcttaccaat atataggaat
     5221 tgttagtaac aattgccttt caaggaggaa tccatccatg ttattaggct cacacgtcag
     5281 catgagcgga aagaaaatgc tgttaggagc agcagaagaa gcagccagtt atggatcttc
     5341 gacttttatg atctatacag gcgcccctca aaacactaga agaaaagcca tcgaagagat
     5401 gaatattgat gccggaaaag cgtacatggc agaaaaaggt ctaacaaata tcgtcgttca
     5461 tgccccttat atcattaatt tggggaatac gatcaaacca gaaatgtttc cttttgccgt
     5521 ccaatttctg agagaagaga ttgttcgtgc ggaagcttta ggcgctactc aaattacact
     5581 tcacccaggt gctcatgtag gggcaggaac agaagccgga attgctagaa ttattgaagg
     5641 cttgaatgaa gttttgacaa aagaacagac ggcacaaatc gctttggaga caatggcagg
     5701 taaaggtacg gaaatcggtc gaacatttga ggaattagcg caaatcatcg atggagtaaa
     5761 attgaatgaa aaattatccg ttacttttga cacctgtcat acgaatgatg ccggttataa
     5821 tattaaagaa gacttttcag gtgtaatgga agagtttgat aagatcatcg ggttagatcg
     5881 gcttaaagta ctccatatca atgattctaa aaatccgcaa ggttcgcata aagaccggca
     5941 cgccaatatt ggttttggaa caatcggatt cgacacgctg aataagatcg tccatgatcc
     6001 aatgtttgac aatatttcga aaatcttgga aactccctat gtaggcaaag ataaaaagaa
     6061 agcttacgca ccatatggaa aagaaatcgc catgttcaaa gcgcaaacat tcgatccaga
     6121 agtgtttcct gagttattag cagaagaagt gatactctaa gattttctat ctgacagcag
     6181 caaaaaagga ccaggcacat tgccttgtcc ttttttgttt aagaaacaaa ggagtacttt
     6241 ttgcagtctt tttcatcatg catcttctcc cccctctttt taaatttaga caaacaaggt
     6301 atactggtag aaaagtaaaa aaacgaaaag tttcacaagc aagtggcaaa acgtgattgg
     6361 aaggacgaaa agatgctgaa cactttaaaa cccttgatca accaactaaa aaagaaacat
     6421 acatacttta ttcttggcat agccctatta tttacaatta ttcgtttgta tttatatagt
     6481 aaagcaatct atggaacaga catgtcggca acctatgatg accagctgct gattcgctat
     6541 gctcaaaata ttttggacgg taaatggcta ggggagtata cgactacaac actttccaaa
     6601 ggaatctcct acagcttatt tttagtactt gcacataaac tgcatctttc ttatgcagtt
     6661 ttgttcggca tgttcagtat ttttgccagt atattggttg cactcgcgtt taaaccgatt
     6721 gtgaagaaca agtatcttct tttaagtatc tatttttatt ttttgttttc tccgattagt
     6781 tttacacatg aatattccac tcgaacatat cgtaatgccc tcgtgattcc ggctgttgag
     6841 atcattatcg cttgtttatt agctatttat taccgcaaat acggttctag cagaaaattg
     6901 ctgccatggt ttttagggct ttctgtcgct tttcctttct tttggttcat tcgcgaagat
     6961 tctatctggc ttcttccttt tactgtagta gctttgggta tcagtttcgt ccagatcttg
     7021 ctagggcaag acttttcttt caaagaagta aagaagttaa aaaaacgact ctttcttctc
     7081 tcgaaacggg aatggctgaa actttcattg tttttacttc ctatagttgg cttttccatc
     7141 gtctatcaat ggatcgaata taaaaatgag acaacatacg gtattgcaac catcaatgac
     7201 cgttccagtg gagaatttgg acgtttgatg aaacaactga tccgaataga tgatgaaacg
     7261 aacttgaata aagaaaacag tgagatatgg gtctcacatg ctgcactaaa taaagcatta
     7321 gccgcatctc ctacattcca tactgtttct acccgtattg acgaattata tacgactcac
     7381 gcgtggactc aaggtggaaa agtaaaagaa ttacccggag atatcatctt ttgggcattg
     7441 cgagatgtgg taagcgaatc tggttattac caaaacaatg caaaagaaac caatgatttt
     7501 tggaaaaaag tcaaccaaga attagaagaa gcctatcaca agggaacaat caaaaagaaa
     7561 aaagagttct atctgatggg atcaggagat ggaaagacga tgagtgatct gccaatcgta
     7621 ttcgatttcc ttcaatcagg atacgcttat gcatggcgat ataaagattt ccatcaagga
     7681 ggaaattata gtttcggaac gcaagaacaa gtcaaacagg ctcaagaatt attaaacctt
     7741 ccgctaatgg agaattggat cgatagtaat gaaccgagaa cacctaattt ccaattaaca
     7801 aaatcagcaa aagtcgcgaa tgtcatcata aaaatttacc aagttctggc tattcccgtg
     7861 ctgattgctt caatagcagg gatattactt atgatactag gaaccgtatt tgcaaaagct
     7921 gatcgtgcct attatagttc ctctttgatt gtggtcaccg gattaatact gacacaattg
     7981 gtttttcttt tcggtgtctc atggttttgt tcttatgcgc cagaaaaaaa ggactatttt
     8041 ttaagtgtct acactggtgc aggcgtgcca ttaacgcaat tcatcgtagt aacaagcatg
     8101 ctagttttca gtaaattaaa tctgaaaaaa gctttatctt ctaaataaaa agacccttgg
     8161 tagctagatt tttaacctaa ctatcaaggg tctttgtgtg gaaaaatcag tttttattcg
     8221 atttcttcta ccgtttcctg caaaagctgt tcatattttt tgtttccttt atataaatca
     8281 ccaatcgtcc atgccaacag gagaaggaca agaacaatga ttacataagc gatgtgatca
     8341 gccaatgcta gttgagcgga agtggcttgc tcgccgaaga aactttcgat ctgtcctggt
     8401 aatcctcgta agttcaagta agttagacta atcacagaga accagccaag aagttttacg
     8461 atgaatgaat ttttaaatcg gctacccatc tccgcttggc tgttggtcat catcagtaaa
     8521 ggaatcatcg aaaacggcaa cgcaaaagcc aagaacacct gggattcatt cattaagtta
     8581 ttcaaagcgg catgttcttc gatcgttcct tttgtacttg taaacaaaac acaaatcaat
     8641 acaggaatca cagaaagcaa tcgtgtgacc agtcttctta gccaaatcgg gatacgcata
     8701 tggatgaatc cttccatgat gacttgaccg gtcaacgtac cagtaatagt agagttctgg
     8761 ccagaagcaa gaagtgctac agcaaataaa gtggacaaga taccagattt tgctacagca
     8821 atcaaaatac cattgctcat agctgatgga tcagataaag cttcatacag accaaaaaag
     8881 gaaggatctt ggacagaacc ggttttgaag acagcgacac ccatgatcaa tagcagtgca
     8941 ttgactagaa aagccattga caactggata ttagaatccc aagtagaaaa acgtacagct
     9001 tgtgctacat cttcttcgtt ttggtgatcg atcttccgag tttgtgaaac tgctgaatga
     9061 agatataagt tatgcggcat gactgttgca ccaataatcc ctaaagctcc agtcagcgga
     9121 gtttcgccgc cgatgaccgg tgagtctgag aatgtatgac tgttagggat aaagccagca
     9181 aataaactgt gccaatctgg gttagacaag ccaacttgat agacaaaaac gaataaaatg
     9241 acgaaaatta agcagacaac cagtgcttcg atcttacgaa acccgacttt tgtcagtagt
     9301 aagagaacga aaacatctaa tacagtaata aagacggcaa tcaccaatgg aatatcaaaa
     9361 agtaagtaga gggcgattgc tgcaccaatg acttctgcaa tatctgttgc cataatggct
     9421 aattcagtca agatccataa aatgacacct aatgtcttac ttgtgcgtgc tcggattgcc
     9481 tgtgccaaat ccatttggct cacgattcct aattttgctg ccatatactg taaaagcata
     9541 gcaatcagac tagacatcaa aataactgac atcaataaat attggaaatt ttgtcctccg
     9601 gtgatcgaag tagaccagtt acccgggtcc atatacccaa ctgctactaa cgcaccaggt
     9661 ccagagtaag caagaagtgt tttaaaaaaa cttttcccct ttggtacatc aactgttcca
     9721 tttacttcag ctaaagataa accatttgta tgttcaataa gtcgatgctt atgaaattgt
     9781 ttattggatt gattattcaa gaaaaaacca cctttcctat ttatctacga ttacattcta
     9841 cacctcaaaa agaaaaaatt aaagaaaaag attcggtacg cctaattttt tttcatggcg
     9901 cgtcttgcta gatgacgaaa gaaattttgt tctcctctaa aaaaaccact attttttgca
     9961 tgaaactaaa gaatacgcct ttcttttttc aataaacttt agtaaaataa gtcattgaga
    10021 gcaattcgga aatagaaagg agagatttat atgttatcaa caggtattgt cgtacttatt
    10081 gctattattg ctttgctaat cggcgcagta ggtggctttt tcctagcgcg taaatatatg
    10141 aaagactatt tagaaaaaaa tcctcccgtt aatgaggaca tgttgcgatc aatgatgatg
    10201 tcaatggggc aaaaaccatc tgagaagaaa attcgccaga tgatgcagca aatgaagaac
    10261 caagggaaaa aataggttct gacccaacga ctttttacgg gctgattttt tcagtccgtt
    10321 ttttatttcg taatttgtta gactattcag attattcagc aaaaaagaca agcacagaca
    10381 atcctaggct cttgataaag acaaaggagg agtaaaatgt cgatttttag aaaattaggc
    10441 tggttttttc gtcaggaaaa gaaacattat ttaattgggg ttttctcgct attcgcagtg
    10501 gccttggtac agttagttcc tccaaaagtg attgggatca tcattgatga gatcgccgat
    10561 aaaaatttat caataaagat cattttcggg tggatcttcc tattgattat cgctgctttt
    10621 ttacaatatc tttttcgtta tatctggcgg acgaatattt ggggaagtgc agctcgattg
    10681 gagaaagaac ttcggcagca gttattcaaa cattttacag aaatggacaa tctcttttac
    10741 caaaaatatc ggactgggga tttgatggct cacgcaacga atgatttgaa tgcgatccag
    10801 aatgtagcag gagctggtat cttgaccttt gcagattcag tgattacggg cggaacaacg
    10861 atcattgcga tgattttgtt tattgattgg cgcttaacac tgattgcgct gattcctctg
    10921 cctttgctgg ccgtcacttc acgagtgctg gggtcaaaac tccatgatgc ttttcgtgat
    10981 tcgcaagaag ctttttctag tatcaatgat aagacacaag aaagtatcac tgggatcaaa
    11041 gtcattaaaa catttggaca agaaaaagaa gatctagcgg attttgaaga aaagattgaa
    11101 gatgcaatta ttaaaaacaa acgagtcaat tttttagact ccttatttga tccattcatt
    11161 accttgatta tcggtctgtc ttatgttgca acaattattt taggtggaaa gtatgtcttg
    11221 gaaggcacga tctcgatcgg gcagatggtc tcatttatta gttatatcgg tatgcttgtt
    11281 tggccgatgt ttgcgattgg tcgattgttc aacgtattgg aaagaggaaa tgcttcttat
    11341 gatcgcgtca atgaactttt gcatgaaaag acgcatatcg tggaacaact gggtgcagtg
    11401 cagaccccgg caaaaggcga gctgaatgta gctatttcct cttttaatta tccgaaagaa
    11461 gaatcaccag cattagaaca aatcaagttt gcgatccaag aaggagaaac attaggtatt
    11521 gttgggaaaa ctggtgcggg aaaaacgacg atcatcaaat tactgatgcg tgaatacgat
    11581 gattatcaag ggaaaatcca atttggcgga cgttcgatca aagaatattc attgaatgct
    11641 ttattaggat caatcggcta tgtgcctcag gatcacttct tgttttcgat gaccattcga
    11701 gataatatcc gtttttctaa tccaaaactt tcagaagaag aagtagagca tgcggcttct
    11761 ttagcattta tcaacaatga aatcaaggaa ttaccaaaag gttatgatac cttagttgga
    11821 gaacgtggag tctctttatc tggcggacag aaacaaagga tttcgattgc tcgagcattg
    11881 atcaaaaatc ctgaattatt aatattagat gatgcactct ctgcagtaga tgccaaaaca
    11941 gaagaagcta tcttgcataa tttaaaagaa ataagaaaag agaaaacaac tatcattgca
    12001 gctcaccgtt taagtagcgt gatgcatgca aaagaaatca ttgttgtcga tcaagggaaa
    12061 atcattgaac gaggaaccca tcatgagctg cttgcactag gcggctggta tgaacaaatg
    12121 tgggagaaac agcaattaga agcgaagata gaaggaggcg aggcctgatg gatgaaaatt
    12181 atcaatcgga atggacaaaa tcgattccac ttaaagaaca gaaaaagatc atcaaacgac
    12241 tgatgaagtt cgccaaacct tttagaagaa cgttcatcgt tgctatctta tttgcgtttg
    12301 ctttgtctgt gatcaatgtc ctattgccaa ggatcatcca aacttttatg gatgatcatc
    12361 tggctaaaca gtcggctacg acacaagtta ttttgttttt tgctggtatg tacttatttg
    12421 gagtcatagt aaaaagtatc atttggtttt tccaatggta tttgtattcc atggcctcat
    12481 tgaagaccta tcagtatatt agggtaaaat tatttgaaaa gctgcataca ctaggtatgc
    12541 gttattttga tcagacaccg gcaggatcaa ccgtttctcg agtgacgaat gacacagaaa
    12601 cgctatttga attttggtac gtctttttga tggtattgac tgggatcttt gcagtcgttt
    12661 cttcattcct tgccatgttt caaatcaatg gagaaattgc cttgtggact ttggtgtttt
    12721 tgccgatttt aggaatcgtc atctggtatt accagaaatt cagttctcgt atttaccgaa
    12781 atatgcggga aaaattaagt cagctgaaca cgaaattgaa tgaatcgatt tccggcatgc
    12841 gtattatcca gcagtttcgc caagaagctc gtttagctaa agaatttgaa caagtgaaca
    12901 atgagtattt agacacgcgt tatgcgatga tcaaaacgaa ttcgattctg ttaagtccga
    12961 tcatcaattt tctatatgca ttggcgattg cgttggtgtt gactttgttt gggatcgatg
    13021 cgttgcattc tcccgtagaa gttggactga tctatgcatt tacgacctat gtccagggat
    13081 tttttaatcc aatgtctcaa atgatggact ttttgagcat cttcacagat gggatggttg
    13141 ctggtagtcg tatcttgaag attatggata cggaagaata tgcaccacaa caaaaccctg
    13201 gagcagatca aatgattact tcaggtaaga ttgagtttcg caatgtaagc ttttcttatg
    13261 atggagagca caatgtatta aacaatatct cttttgttgc taatccggga gagaccgttg
    13321 cattagtggg acacaccgga agcggaaaaa gctcgatcat caacgtattg atgcggtttt
    13381 acgaatttta tgaaggtcag atattgattg atgatcatga cattcgagaa tatccaatgg
    13441 aagagttacg taagaaaatg gggctggtct tgcaagatgc tttcttgttt tatggggata
    13501 tcgcaggaaa tattcggatg atgaatccag ctatcacaga cgaacagata cgccatgcgg
    13561 ctgagtttgt tcaagcaaac aaattcatcg atgaattacc aaaaggatat cactcaaaag
    13621 tgatcgaaca tggtgccagc tattctagtg gacaacgaca gctgatcact tttgcgcgaa
    13681 caatcgtgac tgatcctaaa attctagtat tggatgaagc aacagcaaat atcgatactg
    13741 aaacagaagg attgatccaa gaaggattgg caaaaatgcg tcaaggacgg acaacgattg
    13801 cgattgccca tcggctgtca acgattcgag atgcagaatt gattttagtt ttagacaaag
    13861 gtcatatagt agaacgcggg aatcatgaaa cacttctagc gcaaaatggt ttgtatgcag
    13921 atatgtacaa actgcaaagc agtggtatat aaaagatgtt gtgacagtag aattttgacc
    13981 taagcactag agtgaaaata aaacatagct tctcatattt ttcatctgtg agagttagtg
    14041 tcgtagtcac cagatttaaa aaaagattcg acagctagtc agaagtgcct aatagccact
    14101 aagaaagcgt tgacaaaagc atttgctttc gataatataa agaaaacgat acaacttgga
    14161 tagtgattgc ctgatgcaag ctaaacctga tgagtctccc atggtttcat ggacggcttt
    14221 ttgggttttt ctttttgttt tggatacagc cctatacatc atggaaagga gcaaaacatg
    14281 ctgattgaga aagtactgaa taacaacgtc gtaataacca gggatgaaag aaatcaggaa
    14341 atgatcgtga tgggaaaagg tttggcattc aaaaagaaag tgggtgaccc gatttttgac
    14401 tcatgcatcg acaaaaaatt tcagttgaca aatgacgggc tttcacaaca gttccaagaa
    14461 ttggcacaag cgattcctct tgagtactta gaaatttctt gtgaaatcat tgaatataca
    14521 aaagaggctc tacaagtcca gttgaatgat tctgtgtata ttgcattgac ggatcatttg
    14581 tatacggcta tcgaacgtca caaaatggga atatttgtac caaatgtatt gctgtatgat
    14641 atcaaacaac tatttcctaa agagtatgcc atcgggaaaa aaacagtgaa gaaaatctcg
    14701 gataaatacc acatcaagtt atcagaagat gaagccggat ttatcgcatt gcatttagtc
    14761 aatgcacaag gcattcaaag ccagcaagac aatacctacc tgtttactat aacaatacag
    14821 gatattctaa atatcgtccg ctattactat aagattacat ttgatgaaga ttcagtccat
    14881 ttttttcgat tcgtcacaca cttacgtttt tttctgacac gcgtcctaga tggagaaaaa
    14941 tcagaagtgg gagaagtttc tgaggaattg tttcaggtta ttcaaaaaaa atataaggat
    15001 acgtttggtt gcgttgagaa aatagcagac tttttagccg agacgttcaa ctaccagatg
    15061 tccacggacg aaaaaatgta tttaacgatc catactgcgc gtttagtgca tgagaatcgt
    15121 tgtaaataaa aaaaggagga taaattaaca agaacgtatg aaataagggt agtgacattc
    15181 agttggctaa accttccact aataaattgg agggaataga aatgggaaaa taccaagaat
    15241 tagcgagaaa aatcgtagaa aatgtcggcg ggaaggaaaa tatcaatggt ttgacacatt
    15301 gtatcacaag gcttcgtttc aaattaaaaa acgaagaaaa agcaaatgat gagatcttga
    15361 aaaatatgga cggtattgta acggtaatgc gagcgggagg gcagtatcaa gttgtcattg
    15421 ggaatcatgt gccagtcgtg tttgaagagg ttataaaagc aggaaacctt acttttgatg
    15481 aggctgtttc aacagaaaaa atgagaccgt ttgatatgct tattgatgga atatcgggct
    15541 gtttccagcc attccttgct atcctagctg ctggcgggat gattcgtgga ctgacagcat
    15601 tccttgtctt cttaggcgcg tttgaccgcg gttctggcac atttgttatg tttgataata
    15661 tcggtgattc tgtattccaa tttatgcctg ttattatcgg tttaacagct gcccgtaaat
    15721 ttaaagtaaa tgaattcgta gggatgttgc ttggcgcagc attaatgaat cctagcttgt
    15781 cgctggaagc tttgagtggt gcagcagaag caccactaac gaccatattt tctggcacta
    15841 tttttgaagc acctatttat caaacagtat tcggcattcc ctggattgcg cgtaactacg
    15901 cttcttctgt cattcccatt atctttattg tgttgttagc tagccaaatt cagaaaccaa
    15961 ttaaaaagtt agtacctgag atgatcgcga atttcttcgt tccattcttc accgttctta
    16021 ttacaatgcc tttaggtttt ttactcgttg gaccagtatt cacattcgca actgatattt
    16081 taatggctgg atttgaaaca cttttagcac tttcgccagt tatttatgga gcaatcgtag
    16141 gcttcttctg gcaaattctg gtgatgtttg gtctccattg ggcaatcgta ccaatgggat
    16201 tgatgcaatt ttctgtgaat ggttggcaaa atattatgac acctgttgca gtagtgtctt
    16261 ttggacaaac tgcagcactt accgcactct actttaagct tagaaatcca aaagacaaag
    16321 caattgcaat accagccatt gtttctggta ttgtggggat tacagagcca gctatttatg
    16381 ggtttacatt accacgtaaa aaaatattta tttttacctg tgtcggtggt gcgttagggg
    16441 gcgcgtatag tgggttaatg aatttaactt cttggaatca aggtggacta ggcatcttta
    16501 caattcccaa ctatattaga ccagatggtg atttaacaga tgtgattaat gtattgattg
    16561 ggatcgccat tgcgatggtg gtatccttta cgctgacttt tttcttttgg aaggatgaag
    16621 caggtgaaac agatgatata cagaaaaaat caggtaaaga aattgtaaaa acaccgatac
    16681 aaggacaaat cgctccacta aatgcagcaa aggatgcagc ttttgcacaa ggaacactag
    16741 gtcgaggaat cttgatctac cctgaaaaag gagaagtacg tgcccctttt gacggaacag
    16801 tcatgaccct gttcccgacg aaacaggcaa ttggaatggt ttctgaaaca gggctggaat
    16861 tgctgattca tgtcggattg gatactgtgc agttggaagg gaaatatttt gaaagcttag
    16921 tgcagcaagg ataaagagtg aaacaaggcg atctgttgct acgcttcgac aaaaagaaaa
    16981 tcgaaaagga aggatacagt ttagaaacac ctgtgatcgt caccaattac acggattatt
    17041 tggatatttt ggaaaaccaa ggggagacgg tcgggccaga cgatgcactt atcaccgcac
    17101 tgacctgaac ggttaggtga agaatggact aggcttaact attcttttta ttccacttct
    17161 ttattttcaa aattgaaagg ctctttcatg atggctgagc tgatagggcg tttatcgaat
    17221 ttttgaccga cagaaattag atggactaaa ttctgttgat aattttctgc aaaaacacaa
    17281 ctatataaca cgtctagaag ataacaaatt gatgtattga tcgtaaaatt accaattttt
    17341 gaatacagtc gctcgcgtgt cgtaagtcgc aaagcggctg tactcattgc agatagcgtt
    17401 gaatcaccga tactggtcaa agctaaaatt ggaacatgat tttgtttcaa tatttctgcg
    17461 gtacgaagga tcacattgtt ttcacccgta taagaaatca gaatcccgca catatcactc
    17521 gtgcaattgt acgcttcata gaatgattca ccaaaagtgg tgacaatcgt gacatccatt
    17581 ttgatgcgct tcattttcag catgaaatct tgagaaatca aagtattcga gttactggcg
    17641 aaaatcacga ttttttttgc ttgaagcatt agttgtttgg ctttttgcag ttggtcatga
    17701 tgaatgagag aaagtgtatc atgaatcgtc atctgttcta aagaagccat tttatttgcg
    17761 atcgtcataa taccgtctgt ggtggaaaaa ggaagattcg catctacgtc ttgaaaattc
    17821 ccttcaagat aggctgtttc agctaaaaaa gcattacgaa aactgaccca gccatcaaaa
    17881 cccatttttt ttgctacacg gatgagtgta gaggggtgga cataacatcg ctcagcaatc
    17941 gcttggatgg tcatttcgcc aagtgctgga gaatcgctta agagaaactg gatcacttca
    18001 ttttctgaag gagaaaagga gatttctttg attttttcac gtaataacat gtagatacct
    18061 tcttcttatt tttttacgta aattataact tttttgaaaa tgatttgtaa acggatttat
    18121 actatataac ttaaaataga cattttttga caagatattt acaaaaatcc actagaaaac
    18181 agtaaatttt gtaaatgtta ctggaaagaa acagatttct attgtttgtt ttgtttacaa
    18241 aaaagataat gaaagtgtaa aaaagtaaag gagtgagaac atggcaaaag gaatcaaaat
    18301 cgctacgatt ggtggaggca gcagttacac ccccgagttg atggaaggat ttatcaaaag
    18361 atatgaggaa ttaccgatac gagaaatctg gctcgtagat gtagaagaag gaaaagaaaa
    18421 gcttgagatc gttagtcaga tgtctcagcg tatgtgggat gcttctccgt atgaagtcaa
    18481 gatctatgca actctggatc gggaagaagc attgaaagat gcggattttg ttacgactca
    18541 gtttcgtgtc ggtcaattag atgcacgaat caaagacgag cggatcccta gttattatgg
    18601 gatgattggg caagaaacaa atggtgctgg cgggatattc aaagcttttc gtaccgttcc
    18661 agtcgttttg gcaatcgttg aggacatgaa gcgtttatgt ccaaatgctt ggctgatcaa
    18721 tttcgcgaac ccaagtggga tggtgacaga agcagttatc agatatggca aatgggataa
    18781 agtgattgga ttatgcaatg tgccagtgat ggcgatgatg acagaaccag caatgcttgg
    18841 caagaaaaaa gaagagctga tctacaagtt tgccggtcta aatcatttcc attggcacaa
    18901 agtagcagat aataaaggaa atgaccgcac ggcagaattg attgatcgtt tatacgatga
    18961 agagaatgga ttaccgaaaa atatctttga tatcccgttt ttcaaagaac aattaaaaca
    19021 aatgaacatg atcccttgtg gctaccaccg ctattactat cgtgaagaag aaatgctgga
    19081 acatgcttta gaagaatatc aaacgattgg tacaagagcg cagcaagtga aagaaacaga
    19141 agcagagcta tttgaattat acaaagatcc agaattagac cataaacctg aacaattgca
    19201 aaaacgaggt ggcgcttatt attcagacgc tgcctgtgaa acgattgctt caatctatgg
    19261 aaataaagaa acacagatcg tggtatctac aaagaatgat ggagctgttc ctgacctgcc
    19321 ggcagattgt gtagtagaag tcactgccta tgttggcgca caaggagcga gaaatgtagc
    19381 gtttggttct cttcctgctg ctgaaagagg ctggttgcaa gtaatgaaga atatggaatt
    19441 gctgacgatc gaagcagctg tgactggtga ttatgggaca gctttacagg cgtttacgat
    19501 caatccactt atacgatcag gaaagacagc aaaacggatc atggatgagt tgtttattgc
    19561 acaccagcag tatttaccgc aattcaagga gacaatcagc cgattaaaag aagaaggaat
    19621 cgaagttcaa gatgaactgg caagagaatt atcaggtaat acacatataa tggaggaagt
    19681 ataatgggat tcagaaaaga ttttttatgg ggcggtgcta cagcagccaa tcaatgtgag
    19741 ggcggttatg atgaaggtgg acgcggacta gcaaacgtag atgttgtgcc aatcggaaaa
    19801 gaccgttttc ctgttatcac cggaaagaaa aaaatgtttg aatttgatga ggatcatttt
    19861 tatccagcaa aaattgcaat cgatatgtat catcgttata aagaagatat tgctttgttt
    19921 gcagaaatgg ggttcaagac ttatcgaatg tccatcgcct ggagtcgtat ttttccaatg
    19981 ggggatgaga aagagccgaa tgaagctggc ttgaaatttt atgaggatgc ctttaaagaa
    20041 tgccaaaagc acggaattga gccacttgtg acgattacgc attttgattg tccaatgcat
    20101 ttgatcaaag aatatggtgc gtggcgttcc agagaactcg ttggttttta tgaaaatctg
    20161 tgtcgagtca tcttcaatcg ctataaagga cttgtcaaat attggctgac attcaatgag
    20221 atcaatatga tccttcatgc accattcatg ggcgcagggc tgtattttga aaaaggagaa
    20281 aatgaagaac aaatcaaata tcaagcggca catcatgagt tggttgcaag tgccatcgca
    20341 acgaagattg ctcatgaagt agatccggag aacaaagtag gctgtatgct ggcagcagga
    20401 gcatattatc catacacttg caatcctgaa gatgtttggg cttctagaaa atcagatcga
    20461 gaaaattatt tcttcattga tgtccagtca cgaggagaat atcccgctta tgcattgaaa
    20521 gaaatggagc gaaatggcat tcagatcgaa atggaaccag gggatgccgc attgctgaaa
    20581 gaacatactg ttgattttat ctctttttct tattatgctt cccgagtttc gactaccgac
    20641 aacgaactct tggaacagac tgcaggaaat atctttgctt ctgtcaaaaa tccatatctg
    20701 caagcaagtg aatgggggtg gcaaattgat ccattaggct tgagaataac gatgaatgat
    20761 atttatgatc gttatcaaaa accattgttt attgtagaaa acggcttagg tgcagtcgat
    20821 acgccagatg aaaatggtta tgtggaagat gattaccgaa tcgattacct ggcagcgcat
    20881 atccaggcga tgaaggatgc agtagaacta gacggagtcg atctattagg ctatacgact
    20941 tggggatgta ttgatttagt atcagctggt accggtgaga tgaagaaaag gtacggattc
    21001 atttatgtcg atcgtgataa ccaaggaaat ggcacattag cccgttcaaa gaaaaaatca
    21061 ttcaactggt ataaaaaagt aattgattca aatggagaaa atctttcgaa cgattgatca
    21121 atgatttata aaagtctatt gtttttcagc ggatttttcg agtataataa agctattcga
    21181 gaaacgcaag cagaacaata gacttttttt cgcattggct gtaaagtatg cttacttgat
    21241 taagtaaagg agaatctaca tatgtatgat gtaatcgtta ttggcgcagg tcctgctggg
    21301 atgactgctg ctttatatgc ttcacgttca aacttatccg tattgatgat cgaacaaggt
    21361 gcccctggcg gccaaatgaa caatacggca gaaatcgaaa attatcctgg attcgattcg
    21421 atcatgggcc ccgatctagc ttataagatg tatgaaagtg tctcaagatt tgggacagaa
    21481 aatgcttatg gaatcgttca agatatcaag gatcacggca gccataaaga agtcatctgt
    21541 gaagataaaa gttacgaagg aaaaacagtc attatcgcaa caggatgtat ccaccgtaaa
    21601 ttaggcgtac ctggcgagga agaatttgct ggacgaggcg tttcttactg tgcagtctgt
    21661 gatggtgcgt ttttccgtaa caaacgtcta gtagtcatcg ggggtgggga ctcagcagta
    21721 gaagaagcca tctatttgac acaatttgct tctgaagtag tgatcgtcca ccgtagagat
    21781 gaattacgtg cacaaaagat catccaagac cgtgcatttg caaatgaaaa aatttccttt
    21841 atgtgggata cagtcgttga cgaaatcgtt ggaaacgaaa tggtagtgac aggtgtgaaa
    21901 gcacgtaatg tcaaaaccgg caatgaattt gatttagcag cagacggagt attcatctat
    21961 gttggactgg atccattaac tgcacctttt agagaagcag gtttgacgaa cgaagctggc
    22021 tggattccaa cagacaacga aatgaaaacg aatatgccag gcgtttttgc catcggagat
    22081 gttcgtgaaa aagatttgcg acaaatcact acagctgttg gtgacggcgg tattgctgga
    22141 caacaagtgt ataaatactt ggagtcttta gaagaaacaa cagcataagt ttaaaaagag
    22201 tagaagaacg ggttcaattg ttcttttact cttttttttc gcgtgaaatt tactgaatct
    22261 gatttatgcg aacacgtcca acaccttttg ataaaagaat cctttcttga tttaagcgtt
    22321 ttttggtcct catactttca tatctaataa gtgaatgcta tacttattaa gaaccatatt
    22381 tcagaaagag gtgttatttt catgtcttgg gaacaagtgt atgaacaatg ggtacatgaa
    22441 gaaaatttag aagaaaactt aaaaaaacaa ttaaatgatc tgggtgaaga tcctgaaaaa
    22501 cttgaagatg ctttttatgc accgttggaa ttcggtactg caggtatgcg gggaatctta
    22561 ggacctggga tcaatcgaat gaacatctat acggtaagac aagcaacaga aggactagca
    22621 cgtttcatgg atacacaagg tgaagaaaca aaacgccgag gtgtcgctat cgcttatgac
    22681 tcacgccatc agtcaccaga atttgcaatg gaagcagcca aaacactagc acatcacaat
    22741 atcccgtctt atgtattcga aagtctgcgt ccaacaccag agctttcttt tgcagttcgt
    22801 catttgaaga cgtttgcggg aatcatggta accgcatctc acaacccagc ggcttacaat
    22861 ggatataaag tctacggaga agatggcgga caaatgcctc ctgcagacgc agatgcattg
    22921 acgaaatatg tccgtgaagt cagcaatccg ctaaaagtcg aagtattatc tgacgaagaa
    22981 gctaaacata gcgggttgat cacgatcatc ggagaagaag tggacgctgc ttatctagaa
    23041 gaaatcaaag cagtgacgat tgatcgtgaa ttggttgaaa caatggggaa agatttgaaa
    23101 cttgtgtaca cgccacttca tggaacaggg aaaatgttgg gcgaacgtgc gttgaaacaa
    23161 gctggattcg aacaattcgt actagttccc gaacaagctg tggcagatcc agatttcact
    23221 acggtcaaat cgccaaatcc agaagaacat tcagcttttg aatatgctat acgacttgga
    23281 gaaaaagaag gagctgactt gctgattgca acagatcctg atgcagaccg tttaggcgca
    23341 gcggttcgtt tgccagatgg ttcttatgaa gtattgactg ggaaccaaat cggagcattg
    23401 tttgctcaat atattttgga agcacatcaa tcaagaggca ctttaccaga aaacgcagcg
    23461 gtattgaaat caatcgtttc cagcgaattg cctacagcaa ttgcggaaca ctacggtgct
    23521 accatgttca atgtgttgac agggtttaaa ttcattgctg aaaaaatcca acaatttgaa
    23581 gaagaccatt cacatacttt tatgtttgga ttcgaagaaa gttatggtta tttagttaaa
    23641 ccttttgttc gcgataaaga cgcgatccaa gcattgcttt tattagcaga agtagcagct
    23701 tactataaaa aacaaggcaa aacgttgtat gacggcttgc aaacgatttt tgaaacctat
    23761 ggctatttcg cagaaaaaac gatttctgtg acaatgagcg gacaagaagg ttctgcgaaa
    23821 atctctgctt tgatggaaaa attccgtaaa gaagcaccga cagaatttgc cggagcaaaa
    23881 gttattcaaa cagaagactt taaagaattg acgagaacag accgtgaagg aaacgtagaa
    23941 aaactaacaa caccgccatc cgacgtattg aagtatacat tggaagaaaa tggctggatt
    24001 gctatccgcc cttcaggaac tgagccgaaa atcaagtttt atattggtgt aaaaggaagt
    24061 tctacagaag acgcagccga aaaagtagcc tcttatgaag cgattatcaa aaagatgaca
    24121 gaagaataaa aaacttgaga ctgatggaaa aagtggtaaa ctttagaagt cagtcttttt
    24181 tcaagttgga ggtgaatgga ttgccagaca cgcaaaaatc aaaagtagag aaagtcagtc
    24241 aattattcaa aatgttgagt gatcccactc gtcttaaaat tctgctatat ctgaaagacg
    24301 gggaacaaaa tgtgacagca atcacccaag cagtggaaat ggagcagtct gctgtatcgc
    24361 atcaactacg tttattaaga gaaaatcatg tcgtcaagtc tcatcgcgag ggaaaagcga
    24421 ttttgtatag tcttgatgat tctcatgtac tggatatttt gaaccagaca ctcaaacatg
    24481 tggaacatca ataacgatag gtgcaaatag ataaacactg gagtgtgaca acgaagaaat
    24541 ttcgttgtca cactccagtg ttttttaaga aaaagacaaa aatgatcaat cgcttttttc
    24601 tgttgtttca gttccataat aactatgatc ttctaggtct aaagcattca aaataagcga
    24661 agccgtttct tcaatagata aagatgctac attgatcact acacatccta gtttttcata
    24721 aagatcattc gcaaaatcga gttcagctct aattttttcg atatcggaat aagcagtatc
    24781 agggtttaat ccgtaagagc gcatccgttc tttacggata ttatttaaaa tatctgggtt
    24841 atttgttagt cccacgattt ttttaggatc gatctcccaa agctgtttgg gaatatgtgc
    24901 ttgtgggatc aaaggtaaat tggcaacttt taaatttttg ttagctaaaa acaagctcaa
    24961 aggcgttttg gaagttcggg atacgcctag caaaacgaca tctgcttcta aaaaaccacg
    25021 aggatctttc ccatcatcgt attttacagc aaattccatt gctttgatcc gtttgaaata
    25081 gttctcattc aataaatgga gggcaccagg ttgccgaatt ggcttgatac cagttaaacg
    25141 ttcaatttct gatatcggtg gcgtcatcac atcaaaacta aataattgat gttcttcaca
    25201 aaaagaacgt gttatctcga tcagttcttg attgattaat gtatgcagga caagtgcttt
    25261 ttcttctaat gcatcttgca atgcctgcct taatgttttt tcatctgtca cgaacgttct
    25321 gcggaatagg ttgaactcaa cggtaggata ctgagccata gtagcttgcg ctagttttgt
    25381 cgcagtttct ccagcagagt cggagatgac aaaaaaagta acgatttctg tagaatcgga
    25441 agtcatggat aaaacctcac ttgcattttt tttatgttta ttataccata ctctagaatg
    25501 cgtacttcgt aatcattttg atttggaggg ataagtcatg aaaaaacaga cattgatcga
    25561 acaatcatta gaaacattga aaaataacgg ttttaaatat actaaaaaaa gagaagcatt
    25621 gttaacttat ttagtgaagc gtaaccgcta cgtatctgct aaagaagttt ttgactatat
    25681 gaatcaggaa ttcaaagggg taagttatga tacgatttac cggaatctcc atgattttgc
    25741 agagatcgat ttactggaag aaaccgattt aaatggagaa atgaagtttc gttttcattg
    25801 ctgtcagggg aaaatcggcc atcaccatca ttttatctgt acagtgtgcg gaacaacgaa
    25861 agaaatccat atgtgtccga tgaattattt cgaagaacag ctaggtggat gcacgatcga
    25921 agggcataga ttcgagattt tcggaagatg tgaaaattgt caataaggat ttaaataaaa
    25981 taacatttga taattttaaa aaaaagcaag atttccttga cggtcaaaac agcattagat
    26041 ataatattta caggacagtc ttgttttttg agatatattt caataacaat tacggttact
    26101 ttttaagctc ggagggaggg aattaacatg tcaaaaacag tcgttcgcaa aaacgaatca
    26161 cttgacgatg ctcttcgtcg cttcaaacgt tccgtgtcaa aagcgggtac tttacaagaa
    26221 tctcgtaaac gtgaattcta cgaaaaacca agtgtaagac gcaagaaaaa atctgaagca
    26281 gctagaaaac gcaaaaaatt ctagttctgt gatgaattgg agttggtagt gtgtcactac
    26341 taagtagatt aaacgatgac atgaaagcag cgatgaaagc gaaagataag gaatctttac
    26401 aagtcatccg aatgatcaag tcatctatac aaaacgagca gattaaagaa ggtcacgact
    26461 tgacagaaga agaagaacta acggtgttgt ctcgtgaaat gaaacagcga cgtgattctt
    26521 tgcatgagtt tgaagaagca ggacgtgacg atttagctga aaaagtaaaa agtgaaattg
    26581 taatagtcga aaaatatatg ccggaacaac tttccgacga agaaattcga caacttgttc
    26641 aagaagcaat cactcaaact ggtgcttcat caatgaaaga atttggcaaa gttatgggtg
    26701 caattatgcc taaagtcaaa ggaaaagcag atggaaacca agtgaatgcg attgtgaaag
    26761 aactattaca atcttaattt tatcatttaa actgcggaca gtgattatcc gcagtttttt
    26821 gtgttcgtaa aaaaagaact aaaaaacagc tagaagaaag aatcttattc tt
//
LOCUS       DACDRN010000034        26854 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDRN010000034 DACDRN010000000
VERSION     DACDRN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.034"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(20..367)
                     /gene="rbfA"
                     /locus_tag="IWJ80_001653"
     CDS             complement(20..367)
                     /gene="rbfA"
                     /locus_tag="IWJ80_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP9461369.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(394..2700)
                     /gene="infB"
                     /locus_tag="IWJ80_001654"
     CDS             complement(394..2700)
                     /gene="infB"
                     /locus_tag="IWJ80_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP9461370.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(2713..3024)
                     /locus_tag="IWJ80_001655"
     CDS             complement(2713..3024)
                     /locus_tag="IWJ80_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP9461371.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(3021..3314)
                     /locus_tag="IWJ80_001656"
     CDS             complement(3021..3314)
                     /locus_tag="IWJ80_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP9461372.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(3336..4511)
                     /gene="nusA"
                     /locus_tag="IWJ80_001657"
     CDS             complement(3336..4511)
                     /gene="nusA"
                     /locus_tag="IWJ80_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP9461373.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(4535..5008)
                     /gene="rimP"
                     /locus_tag="IWJ80_001658"
     CDS             complement(4535..5008)
                     /gene="rimP"
                     /locus_tag="IWJ80_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP9461374.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(5152..9504)
                     /locus_tag="IWJ80_001659"
     CDS             complement(5152..9504)
                     /locus_tag="IWJ80_001659"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP9461375.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(9716..11425)
                     /locus_tag="IWJ80_001660"
     CDS             complement(9716..11425)
                     /locus_tag="IWJ80_001660"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP9461376.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(11493..12761)
                     /gene="rseP"
                     /locus_tag="IWJ80_001661"
     CDS             complement(11493..12761)
                     /gene="rseP"
                     /locus_tag="IWJ80_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP9461377.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(12922..13722)
                     /locus_tag="IWJ80_001662"
     CDS             complement(12922..13722)
                     /locus_tag="IWJ80_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP9461378.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(13719..14531)
                     /locus_tag="IWJ80_001663"
     CDS             complement(13719..14531)
                     /locus_tag="IWJ80_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP9461379.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(14726..15283)
                     /gene="frr"
                     /locus_tag="IWJ80_001664"
     CDS             complement(14726..15283)
                     /gene="frr"
                     /locus_tag="IWJ80_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP9461380.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(15286..16008)
                     /locus_tag="IWJ80_001665"
     CDS             complement(15286..16008)
                     /locus_tag="IWJ80_001665"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP9461381.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(16144..17025)
                     /locus_tag="IWJ80_001666"
     CDS             complement(16144..17025)
                     /locus_tag="IWJ80_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP9461382.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(17124..17906)
                     /gene="rpsB"
                     /locus_tag="IWJ80_001667"
     CDS             complement(17124..17906)
                     /gene="rpsB"
                     /locus_tag="IWJ80_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP9461383.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            18265..18744
                     /locus_tag="IWJ80_001668"
     CDS             18265..18744
                     /locus_tag="IWJ80_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP9461384.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            18961..20052
                     /gene="phnW"
                     /locus_tag="IWJ80_001669"
     CDS             18961..20052
                     /gene="phnW"
                     /locus_tag="IWJ80_001669"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP9461385.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            20045..20830
                     /locus_tag="IWJ80_001670"
     CDS             20045..20830
                     /locus_tag="IWJ80_001670"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9461386.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(21176..22867)
                     /locus_tag="IWJ80_001671"
     CDS             complement(21176..22867)
                     /locus_tag="IWJ80_001671"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP9461387.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(23289..24236)
                     /gene="arcC"
                     /locus_tag="IWJ80_001672"
     CDS             complement(23289..24236)
                     /gene="arcC"
                     /locus_tag="IWJ80_001672"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP9461388.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(24351..25370)
                     /gene="argF"
                     /locus_tag="IWJ80_001673"
     CDS             complement(24351..25370)
                     /gene="argF"
                     /locus_tag="IWJ80_001673"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP9461389.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(25461..26690)
                     /gene="arcA"
                     /locus_tag="IWJ80_001674"
     CDS             complement(25461..26690)
                     /gene="arcA"
                     /locus_tag="IWJ80_001674"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP9461390.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         7151 a         5792 c         4772 g         9139 t
ORIGIN      
        1 gatttgcggg attttttgat tagtctttgt tcaagtcgcg aagcaattca tcgatatggt
       61 tcccatagac aactgattca tcaagttcaa agaacaattc aggtgtctta taaatactta
      121 gattgtgccc taactctcga cgaatcaatc ctgatgcttt attcaatcca tcttgtgctt
      181 ttttcttatc tgatgctaag tcagataaaa tgctgtaata gatcgttgct tgctgtaagt
      241 cccccgtaac tttcacatct gtgatcgtta cgttctccac acgagggtca cggacctttt
      301 tgcgcaaaat atcattgact tcttttaaga tttcttgacc gactcggcga tcacggtagt
      361 tagccataat gattggcctc cttttatcag tttttagtca tttttgactt cttccatgat
      421 aaatccttca attacgtcat ctactttgat gtcattgaat ttttcaatca tcgcaccaca
      481 ttcataaccc attttgactt ctttcacgtc atctttgaat cgtttcagac ttgcaagttg
      541 gccttcatag attacgattc cgtcacggat tacacggaca ccgctatctc tacgaatata
      601 tccttctgtt acataggcac ctgcaatcgt accaacttta gaaactttga aggtttcacg
      661 aacgatcatt tgaccggtga ttttttcttc aaattcaggg tctagcatcc ctttcatcgc
      721 agtttcgatt tcttcgatgg ctttatagat gatacggtga agacgaatat cgacttcttc
      781 agtatcagct tgcacttttg cctgcggtgt aggacgaacg ttgaaaccga tgataatagc
      841 gttgctggct gctgctaagg ttacatcact ttcattgatt gctccgacag ctgaatggac
      901 gatttttaca cggacacctt ccacatcaat cttcttcaat gatgcagcca gtgcttctgc
      961 tgaaccttgt acatctgctt tgatgattac gttaacttct ttcaattcgc cttctttcag
     1021 gctttcaaat aagttgtcca aagtcacacg gcttgtggca gcacggtttt ccaataatgc
     1081 acgttttgcg cgttcttcac ccgctgcacg tgctgtcttt tcgtcttcga agactacgaa
     1141 acggtcacct gcttgaggta catcattcaa gccagtgatt tctactggcg ttgcaggtcc
     1201 tgcttcttta tcgcgacggc caagatcgtt tgtcattaca cggacacgtc cgtaagtatt
     1261 accgacaacg attgggtcac caacgtgaag tgttccttct tggacaagta aggttgacac
     1321 aggaccttta cctttatcaa gacgtgcttc gatcactgtt ccgattgcgc gttgtgttgg
     1381 atctgctttt aaatcttcta cttctgcgac taacaggatc atttccagca attcttcgat
     1441 gttttggccg aatttcgctg agatctcaac aaaaatcgtt tctccacccc atgcttcagg
     1501 aattaattca tactcactta attcttgcat aacatgctga ggatttgcac ctggtttatc
     1561 aattttattt acagcgacga tgattggaac gcctgcagct ttggcatggt tgattgcttc
     1621 aactgtttgt ggcatgacgc catcatctgc agcaacaact aagatcgtga tatctgtgat
     1681 acttgctcca cgtgcacgca tacttgtaaa ggccgcatgt cctggtgtat ccaagaaagt
     1741 gattggttta ccatcaatat cgatctggta ggcaccgata tgctgtgtga ttccgccggc
     1801 ttcgccgctt gttacacgtg aatggcgcaa tgtatccaat aaggttgttt tcccgtggtc
     1861 tacgtgtccc ataatcgtta ctactggcgg acgtgagact aatttatctg gatcgacttc
     1921 ttctgcctcg aagaatttat cgatatcagc aatgtcaact tggacttttt cttgtggttc
     1981 catgccatag tccgtcgcta acagttcaat cgtatcttta tccagtgctt ggttttgatt
     2041 gaccatgaca ccaagcataa ataatttttt aatgatttct gctggctcac gatgaatttt
     2101 tttcgcaata tctgctacgt tcataccttc agtatactct aatacatctg gcaattcacg
     2161 gaatttgcgt ggtggtacag ctggtttagt tgattgttgc tgtttgcctt ttttaccttt
     2221 tttattataa cgattacggt tattattatt aaatttgccg cgattgttat tgtttggttt
     2281 gttgttattg ttagtacggt tttgattgcc gccttggctc gttccttgac gattttggct
     2341 gctggtattt cccgtattat tttggtttgt tgttggtttt tttccttgat tcacttgacc
     2401 gctcccttga ccgcgatctt gataactgcg gttattttta tttttttgtt gattgttcgt
     2461 tgtttctttt ttacgttgat cgttcggctg ttgattggct ggttttctgc ttgcttgttg
     2521 tgcagcttgt tgtttcgtgc ttgggttttt gaacgcttgt tgcaatttct tctcatcact
     2581 gctagtaatc gcacccatat ggtttttcac atcgatgcca agttgttgtg ctttttccac
     2641 gacgtcttta ctagattttt tgagttcttt tgctaattca tagattcttt tgttgcccat
     2701 gcaatcacct tcctaacctt caattagctc cttggtcttc ttcgcaaatc cggcatccat
     2761 tattccgaat accttgcgag gttttccgat catctgcgtg atttctgctt cagaaaatag
     2821 ctcaaagcaa ggaacttcat aatatgaact cttatctttt atcttttttc ttgtattctc
     2881 acttgcatcg gttgccacaa acaccaattt tgctttttgt ttgcggattt cagcaatggt
     2941 cagttcttca cccgtgatta gttttcccgc gcgcatcgct agaccgatga gattcatggc
     3001 tttttgccga ttaactgtat tcattttcca aataactctt tacgtgcttt ttggtgagtg
     3061 acatagtcaa gaagttcttg ataaaactca tctgttaatg ttgtttcgag tactcgatcg
     3121 agaatttttt tatcccaagc ttgttgtact tcttccggct caagtgagac atacgctcct
     3181 cgtccaggca tttttccagt tggatcgatc gatacttccc cttcttttga acgggtgatt
     3241 cggaccatct cttttttagg tttcatttca cctgaaacca cggatttgcg taaaggaatt
     3301 ttcctttgtt tcatctcttt gcctccttac taaagttaag cttctggttt ttcttcgacc
     3361 gtttcattgt tgaatgcaat tgtttcatat tcatcgtctg ttaaatctga ctgaacgatt
     3421 gcttcatcat gcagttcttc tgtttctttt tgttgttctt gattttcata aaattccgtc
     3481 atgtcagatt ctgatttgat atcgatttta tggttagtca atttagcagc taaacgtgca
     3541 ttttgtccgc gtttaccgat tgctagcgat aattggtagt ctggaacgac aactgtgcag
     3601 gcctttggat ttgtctcatc gaagatcaca tccatgactt gtgcaggatt caatgcattt
     3661 gcgatgaata cagctggatc ttcattccat tcgacgatat ccatgttttc gccttttaat
     3721 tcattgacga ttgcttgaac tcgttgtccc tttggcccga cacatgtacc gactgcatcg
     3781 atatttggat catttgagcg aactgccact tttgaacgat cgccagcttc acgggcgatg
     3841 ctgacaattt caacgattcc atcatagact tctggtactt cttgttcaaa taagcgacgc
     3901 aataggtctg gatggctgcg gctaacaaat acttgcggac ctttagacgt attttctact
     3961 cgggaaacat aaactttgat tcgatcatgt ggttgataaa attcatttgg catttgatct
     4021 tgtttagaaa gaactgcctc aattttacca agattgacat aaatataacg tttatcctgg
     4081 cgttcgacga ttccttgcat gatgtctttt tcataagcac tgaattcgtt ataaataatc
     4141 gttctttctg cttcacgtac acgctgtaaa ataacttgtt tagccgtttg cgcagcgatc
     4201 cgtccaaaat cttttggtgt tacttcaaaa cggatcgtat caccgatctc atatgctgga
     4261 ttgattaaaa gtgcatcttt caatgagact tccaactgtg aatccatcac ttcttccgtt
     4321 acttctttta ccgcatagac atggatcttt ccttttttct gatcaaattc cacttcgacg
     4381 ttttgtgctt ggccatagtg acgtttgtat gcagaaacca atgctgcttc taaagcatcg
     4441 atcacgattt cttttgagat ccctttttca gcttctaaag cgtctaacgc atttaacatt
     4501 tctttactca tttttttctg attcctctcc gtgattaaaa ttggatagct aagcgagctt
     4561 ttgcaatatt ttttcggtca aaagtcaatt ctttttctcg tgtttttatg cgtattttca
     4621 atgtaagctg ttcagcatca aatgattgaa gaaatccttc atattgtttt tcgccatcta
     4681 ctggttggta taaggagaca tggacataat caccaagtgc cttttcatag tcgctttctt
     4741 ttttcaatgg ccgctctgct cccggggagg atacctccag gaaataagct tgaggaatcg
     4801 gatctggatc tgctgcatca agtttttcac tcagtttctc actgacaaag gcgcattctt
     4861 cgatatcgat accgccttct ttgtcaataa atactcgtaa gaaccagctt tttccttctt
     4921 ttacaaattc gacttcgaca agttcaaaat tttgttcatc taaaatcggt gtcaccattt
     4981 ctgtgactgt ttcaacgacg ctactcaaaa gtttcgcctc cttaaaaatt aagtgcgtaa
     5041 ttccattaaa aagagtgagc gaaaaatccg ctcactcaca gttagtatca ttacctataa
     5101 gaactatacc atactctgct taaaattaca agcagacagg aaaacagatg cttatagcat
     5161 atcgaataac gataattgat tttcatctgg tagatctttc aatacgccat tgtcgttcat
     5221 atattcgatc aatgtttttg aaactttacc gcgggtggca agatcttctt tggataaaaa
     5281 tggaccgtct tttcttgctt caacgatttg tttcgccacg ttggttccca aacttgggac
     5341 agctctaaat ggtgcgatca acgtatcacc ttcaataaca aagttaacgg catccgattt
     5401 atacaaatca atcattccaa atttgaatcc tcgttccagc atttcattgg ctaactccag
     5461 cactgtcagt tgattctttt cttttacgga agcatctaat cccttgtccg tgatttcttt
     5521 cattgcttgt ttcacagcat cttttccttt gcacatggag actagatcaa agtcgtctgc
     5581 acgtacagaa aagtatgcac agtaatacaa aatagggaaa taaaccttga aataagcgac
     5641 acgaagagcc atcaatacgt aagctgcagc atgggctttc ggaaacatat atttgatttt
     5701 tgaacaagaa tcgatatacc agtcgggcac attattttct ttcatcgccg atagataggt
     5761 ttctcgcaat tcatcaggga tcttgttcca ttgtccttta cgaacagttt ccatgatttt
     5821 aaatgccatc ccgctatcta atccagcatg gatcaaataa accataatat catcacgaca
     5881 gccgattact tccgccagtg tcgcatctcc tcgtcgaatc aattcttccg catttcctaa
     5941 ccatacgtct gtaccatgag atagtccgga aatttgcagt agttctgcaa aagttgtagg
     6001 atgcgtttct tctaacatcc ctcgaacaaa acgagtgccg aattctggaa tacctaatgt
     6061 ccctgttttg gaataaattt gttcctgact tactccaagt acttcaggac cggcaaatat
     6121 ccgcatcact tccgggtcat ccgtagggat cgtttgtggg tcgatccctg ataaatcttg
     6181 aagcatccga ataacagttg ggtcatcatg ccctagaata tctaatttca atacattatc
     6241 gtgaatcgag tggaaatcaa aatgagtcgt tttccattct gaattttgat cgtctgctgg
     6301 atattggatc ggcgtaaaat catagacatc catataatcg ggaataacga tgatcccccc
     6361 tggatgctgg ccggttgtcc gtttgactcc agtagcgcct tttgctaatc tgtcgacttc
     6421 tgcacttcga aactgcagat tattgtccct ttcgtatcct tttacatagc catatgctgt
     6481 tttgtcggcg actgtaccaa tcgttccggc acggtaaaca tagtcttcgc caaacaatac
     6541 tttcgtatag ttatgggctt ctgcttgata atcacctgag aagttcaaat caatatcggg
     6601 cactttgtct ccatggaatc ctaaaaatgt ctcaaatgga atatcatgac cgtctttgtt
     6661 taagcgtgca ccacattttg gacattgttt ttctggcatg tcaaatccag aaccatacgt
     6721 accatcttca aaaaactcgg aatattggca ctctggacaa taataatgag gggctaatgg
     6781 attaacttca gtgattcctg tcatcgtcgc aacaaagcta gagccaacag agccacgtga
     6841 acctactaga tagccatctt cattactttt atgcactagt ttttgggcga tcagataaat
     6901 aaccgagaag ccattaccat taatagaatt caactctttt tctaatcgtt tttgaatgat
     6961 ctcaggtaac ggatctccgt acatttgttt tgcttttgtg taactcaatt ctgatatttc
     7021 atcttctgaa cctggaatct tcggtgtata aagttcatct ttgactggaa tcacttcttc
     7081 gcagatatct gcgattttat tcgtattttc tactacgatc tctttcgctg tttcttcccc
     7141 taaaaagtga aatgcagtta gcatctcatc ggttgttcgg aaatggacat ccggcaaact
     7201 atgacgattc aacggattcg ctccgcccat agaattgatc aaaatttttc ggtaaatggc
     7261 atcttcttcg ttcagataat gtacattacc tgttgcaaca acgattttat ccaaactttt
     7321 tccgatttct accaaatttt ggataatttc ttccaaatca tgttcatttt ttaccaattc
     7381 ttgctcgatc aaaggcgcat agaccgcttt tggcataact tcaatataat catagaattt
     7441 tgctcggttt cttgcctctt ctaccccttt ttgcatcatg gcttcgaata tttctccctt
     7501 atcacaagct gagccgatca atagattttc acgcatcttt ttcaattggg agcgtggtat
     7561 tcgtgggacg cgttcgaagt attcgacatt cgacattgaa atcagtttga acaaatcttt
     7621 cagtcctgcc tgattttttg ctaaaatcgt tacatggaaa ggtcgagccc ttttgtaaga
     7681 atctccttct ccgatatgct catttagctg atcatgataa agcatgttat gattgtccat
     7741 tgcctctttc acaaagatcc atgctaagtg accagttgct tcagcatcat agattgctcg
     7801 gtgatgctgt tccaagctaa caccaaactt tttagataac acacctaatc cgaaacgttt
     7861 gaattgagga tacagatatc tagctaattc caacgtatca atgactggat tggccgcttc
     7921 aggaatacca taccttgcgt agctagtatt caagaacccc atatcaaaag ccgcgttgtg
     7981 ggctaccaaa attgtatctt ttgaaaattc aaggaacatc cgcaatactt cttcttccga
     8041 ctttgaccct cgaaccattt catccgtgat accagtcaga tctaccgttg ttcgtgacaa
     8101 tggatgtcct ggatcgataa attcatcaaa gctttcgatc acatttcctt tatacatttt
     8161 gactgcggct agctcgatga tcgtgtcata tactgcagac aatcctgtcg tttccacgtc
     8221 aaatacgacg tatgtggctt cactcaatgc ttcatgtgca tcattataag caattgggac
     8281 accatcatct acgacattcg cttccacgcc gtataagatt ttcacacctg ctttttttcc
     8341 agcactatgt gcttccggaa aggcttgcgc accaccatgg tctgtaatgg cgatagctcg
     8401 gtgtccccat tttcctgctt gtgccacaag atcagagatg ctgtttgttg catccatcgt
     8461 actcatattg ctatgaacat gaagctctac tcgtttttcc ccttcaggag cataatcttt
     8521 gcgaggtgta tgtttgactt ctatgatatc ttgcgcattc atgaccaaat cacggacaaa
     8581 cgtatcttct tgaatacttc ctcgtacctt taaccagcta ccgacactga tcgcatcaaa
     8641 tacttgttca tctttttcgc cgttcgagaa ttttttgacg ataaatgatg aagtataatc
     8701 tgtgattttc aaagtcaaga tcttgcgttt tgaacgtagt tctctgactt ctttgtcgaa
     8761 tacgtacccc tcaagtgtga ctcttcgttc ttcttcaacg atactgatca ttggtgtcgt
     8821 tggttcatcg gctggaatat ttcgtcctag ctggatgggt ccttcaagag ctggcgattg
     8881 ctcttttctt tcctttttct tttgttcgtg gacaactaga ctttcagctg cttgcttcat
     8941 aaaggcttct gcctgttctt gtttgcgttc ttcaaaaagt ttcaatacac gttccgcttg
     9001 ctgctcatcc acttctggtt cgatacgaaa ttttgggaat ccgtaactga cgaacaactc
     9061 ttctactaat ggcaagtatt gttgcttcaa ataagaaata gctcctgtac tgtcgattgg
     9121 caagatgact ttacgatctt ttatcacagg tacctgcgtt ttcaatactt gctgtgctag
     9181 tggtgtatcg cattgctgat tttccaaagc ttgggcccag taatcttgca agagctgttc
     9241 atcgaatgtc tggtcatctg ctaagatatt cagtttgact tgtgcaatat ctttaaaagc
     9301 gatagtcaga tgctgagtca atgaccgata tagcataata ggcaaaattt tggaaaaatg
     9361 tagcgtaaat tcccataaac gcgatttacg atggaccact acactatcca tttcaccagc
     9421 agtaagcagt ggatgatttt tttctgcttc ctctagttgg atttgttcca acagtttttc
     9481 aaataaatct cgtttttctg acaaagatat acgccctttc tgatgagaaa caatgcaatt
     9541 gatcgcatct tatcctcttc tttttcagta ccttttagta tactcgtttt ttctgagttt
     9601 tacattaaaa aattggaact gcgattcaaa tcgcagttcc aattttaaaa gcgaatcatc
     9661 agattgtcaa aaacagctga ttcagctatt tttattttct gtgttttaac gcttattatt
     9721 cgccttggtt caagaggatt ggtaaagtat tcgccaattc ttctttacgt acttcaacca
     9781 tttcgccagt tttcttgatt ttcacttcta cgattccatc cactgctttt ttaccgacag
     9841 tgattctgat tggacagcca atcaaatcag aatccgcgaa tttcactcct gcgcgttcat
     9901 ttctgtcatc aaccagtact tcatagccag cttttgtcat cgcttcttct agttcattcg
     9961 tcaggcttgt ttggtattcg tcttttagat tcatttggac gatatgcaga tcaaatggcg
    10021 caattccttt tggccaattg attccttgtt catctgaatg ttgttctacg attgctgata
    10081 ataaacgact tacaccgata ccataacagc ccataatcac aaatttttct cgtccatttt
    10141 cgtctagtac agttgctccc atagattcac tgtaacgagt gccgattttg aatatatgtc
    10201 cgatttcgat tcctcgagta aaggcaagaa caccgttgtt gtctggtgaa ggatctcctt
    10261 cttgtacaaa acgaagatct tcataagaaa gcacagtgaa atcgcgtttt ggattcacat
    10321 ttttatagtg gaatcctgtt tcgttcgctc cagtaatggc attcgctaaa tcttggacat
    10381 aacggtctgc atatactttt acgtcatctg aaacaccgac tggtccgaca gaaccaaact
    10441 cagcacctag atatttttgt gcttcttctt ctgttgcttc ttctaaaaag tcagcagata
    10501 aaaagttttt cagttttacg tcattgactt catggtctcc acgtaccaaa acaaggacag
    10561 gttgttcatc cgcaatgaac aatacagact tgatgatttt ttgaggttca acagaaaaga
    10621 aatctgctac ttcgtcaatt gattttacgt taggtgtagc tactttctcc aattcaagct
    10681 gtgtttcatg cgattttttt gatacatagt agcttgtagc catctccaag tttgcagcat
    10741 aatcactttc agtcgaataa cagatcgtat cctccccgat ttctgagatc gccatgaatt
    10801 cctttgagtc tttcccgccc attgcgccgc cgtctccgat gatcgagcga aattctaagc
    10861 cacaacgctt gaatacttcg gtataggctt tttcgtaatc tttatatgtc tcgtccaaac
    10921 tttcttccgt tgcatggaaa gaatatgcat ctttcatgat aaactctctt ccgcgaagca
    10981 aaccgaagcg aggtcgtttt tcgtcacgat atttaggttg gatttgataa agatttaaag
    11041 gtaatttctt atatgaattg atttcattac gcatcaaatc agtaaaagtt tcttcgtgag
    11101 ttggtccaag gatcattttt cgatcattgc ggtctttgaa acgatacaag tttggtccat
    11161 atgtttcgta acgcccggat tcttcccaaa gttctgctgg caagatggca ggcatcagca
    11221 tttctaccgc tccgattttt tcaaattctt cccgcataat gcgtttcagt ttttccaata
    11281 cacgatttgc taatggcaga tatgaataaa tacccgctga tacttggcgg atatatccag
    11341 ctcttagcaa gatttgatgg cttaatactt ctgcatcatt tggaacttcg cgtaatgttg
    11401 gaatcaacat ttttgattgt ctcattctat atactccttt atttcctcaa aatagttatc
    11461 gtttgttatt ataccctatt tgtcaacttt ttctagaaaa agaatcgttg aatatcgttc
    11521 catgtaacta gcaccatcaa caacattaaa aatccaaagc cagctaatgt caatattcct
    11581 tctttttctt gacttaatgg tttcccgcga atcccttcaa aaatattcaa gactaatttc
    11641 ccgccatcta aggcaggaat cggaagcaga ttgacgattc cgagattcat tgaaagaagc
    11701 gccatcaaac taataacagt tgttacacct tgatttgctg cttctgaaga caattggtac
    11761 atcatgacag gtccgcctaa tttatctaaa ctgaaacctg taaatagtga tcctaaggct
    11821 ttgaaaattt ccaatgaacc gctgaaagct tgacgcgtcc ctccaatgat cttatccata
    11881 aaacctgtat tcattggggc ttttatacca agttggccaa ctttttcgcc atttgactct
    11941 acagatttcg gtgtcacgtc gacagattgc acctgacctt ctcgttcgat cttaaagtct
    12001 aatgttttac ctggattttt cgtaatgact gttgtcagat cattccatga atggatttct
    12061 tttccgtcca cactgactac ctcatcattt tcttttaagc ctgcttctgc cgcagctcca
    12121 ttaggcatga tctctcctac gcgattagtg ttcgtcactt gaacaccacc ttgcataaat
    12181 gccagaatga tgaataacac gattgccaaa ataaagttat tcatgggtcc agcaaagttg
    12241 gtcagcatac gctgccacaa tttagccgat tggaactgta cgtcttttgg cgcaattctg
    12301 atttctgttc cgtcgttttc aataatcgtc gcatcgtgat caacagggta ggttactact
    12361 tctgtttcgt ctccattcac ataaccagtg atagtcagct catcttctaa atcataacgg
    12421 ctcaattcca tcgggatagc atttgtcaat tggattttct tacttaaatt tatcttctct
    12481 acgatgccgt cagaatttag taaaagagaa agaggcattc ctggtgccat ttctgtttca
    12541 tcatccccat tgcctgccat tcgaacataa ccgccaatag gtaataggcg aagcgtatat
    12601 gttgtcccgt cttttgcttg atgcccataa attttcggac ccataccgat agcaaattcg
    12661 cggacgagga ttcctgatcg tttcgcaaag aagaaatgac caaactcatg aacaatcact
    12721 aatataccaa aaacgatgat aaatgtcaga atcgttttca tagctttttt ctcctttact
    12781 tgttttttgt gttttttata aaacaacgat aaaacactat aattttacca tagtatacca
    12841 aagacaccaa tagtatcaat ataatgaaaa ataaaaacct gcgtctttta tgaaattcac
    12901 aaagaaacgc aggtatactt actaaataat gccaaataaa tgcatgatag gaaatacaaa
    12961 cagcaaactg tcgaaacggt ctaagattcc accatggcct ggtaagatat ttcccgagtc
    13021 tttcacatca taatgacgtt tgattgcaga ttcgaccaaa tcaccaaatt gtccgactgc
    13081 tgaaaaaata atcgtcatca gaagcatgac aaacatacca tgattgaata gttcttttgc
    13141 tgggtatagc aataaataaa gaaatgcgac tacaacagca ctaccgatac caccgagcgc
    13201 accttcaatc gttttgttgg gcgaaatatc aggacttaac ttatgctgac cataacgtcg
    13261 accaaccata tatgctccga tatcagttgc ccaaacaata aatagaccaa atatcaaaac
    13321 agccaagctt tctgttcgtg cattgacaaa attctggaaa cctattccca catacaagct
    13381 gacaagcaca ggaaaacctg cttcatcaat cgtatacgta tttttagaaa taactgatgt
    13441 tcctaaaaga atcataacta taagataaaa taacataaat gtacttacat tctctggaag
    13501 aaagaaaaac catctttctt ttggcaaaac aagtaacact gcaccaacgg cggataaaac
    13561 tccttcaaaa cttaagagtg tcaatccttt catccggaac agctcgtaaa ctcctactac
    13621 cgctaagatt gctgctgtaa gctcaaccgc tatgcctccg atccatataa tcggaataaa
    13681 caagactagc gccacagctg ctgtgatcac acgttgtttc atgactgatc ccctttctca
    13741 tcttctgttt tttttacacc gccgaatcga cggtctcgat tttgataaga ggcgattgct
    13801 tcttcaagat gcgcaccatc aaaatcaggc caaagcgctt ttgtaaaata cagctcacta
    13861 tatgcgatct gccataacag aaaattacta attcgctctt ctccacttgt acggatcatc
    13921 agctcaggat cttgtaactc tttaggcaag aaacctgtca ttaaatgctt agcaatcaat
    13981 tcatcatcga tttcttctgc gtgtatttct ccttttgcta cttcttcggc aatttcttgg
    14041 acagcagtca cgatctctgc tctacttcca taattcaaag caaaattcaa taccattcca
    14101 gtattgtttt ttgtttgctc aattgcacgt cttacagcat cttgcgtatg ttctggcaat
    14161 acgttttcat aacccatgac atgaactttg acattttctt taatcaattc tggtacgaac
    14221 gtatcaaaga agtcgacagg tagttgcatg agaaaactta cttcatcttt tggtcgcttc
    14281 cagttttcag tcgaaaaggc ataaagcgtc aacactttca ctccaagcct tgatgctttt
    14341 tttgtgactt tctttactgt ttccatgcct tctttatggc cagcaactcg tggcaaacga
    14401 cggttttgcg cccaacgacc attgccgtcc atgatgatgg caatgtgctg aggaattggt
    14461 ccatcggtat taaattgaaa ttcgcttttt tcttgaatat atttgttttt ttgcggaaaa
    14521 aaacgtaaca ttctttttcc tcctatactt aaaagattcc cactatctta agtattatag
    14581 caatatctaa ctgtttttcc tatgaaatca ttgttttttt atttaaaaac tgaataattt
    14641 tttctttttt taatttttaa ataaaagaaa tataaataaa aacagaggaa gcggaatgat
    14701 ccgcccctct gtttttatga ttttattaaa cttccaataa ttctttttct ttatcagcag
    14761 cgatcttatc tacttcttta atgctgttat ctgtcaatgt ttgtacgtct ttttctaatc
    14821 cgcgcaaatc atcttcggta atatcaccat ttttttgttg ttttttatat tcatcgatcg
    14881 catcacgacg gatgttacga atcgcgattt ttgcattttc tgcttctttt ttcacatctt
    14941 tagctaattc ttttctgcgt tcttctgtca gttgcgggat aactaagcga atgacatttc
    15001 catcattggt tggactgatt ccgatatcac ttgccataat agctttttca acatcttgaa
    15061 gaatgctctt gtcaaaaggt gtgatcatta aaacacgtgc ttctggaatt tggattgatg
    15121 ccatttggtt caatggtgtt ggcgcaccgt agtagttcac agtaatacgg tccaaaaggc
    15181 tagcgttcgc acggcctgcg cggatttgcc caagttcacg ttgaagattt tgtgctgctt
    15241 ttttcatttt ttcttttgct tctgtcataa ttgcatctgc catttttatt tcccccttac
    15301 agttgttccg atattttcgc ctaagattgc acggcgaata tttccgtgtt catttaaatt
    15361 gaagacaagc agtggaatat cattatccat gcttagtgaa cttgcagttg aatccatgac
    15421 ttgtaatcct ttggcaataa cttccagatg agtcagttct tcaaatttta ctgcatttgc
    15481 atctactttc ggatcagcag aataaacacc gtctacattg tttttagcca tcaggataac
    15541 atctgcgccg atttctgccg ctcgtaatgc tgctgttgtg tctgtcgaga agtacgggtt
    15601 gccggtaccg cctgcaaaga taacgatccg tcctttttct aaatgacgtt ctgctttacg
    15661 gcgaatatat ggctctgcga tttgacgcat ttcaatcgaa gtttgcacgc gtgtcggtac
    15721 acccacgttt tccaacgtat cctgtaaagc taaggcattc ataactgtag ctaacatacc
    15781 catgtaatca gcttgagcac gttccatgcc catttgtgct ccgatttgtc cgcgccagat
    15841 atttccgcca cccacaacga ttgccatttc aacgcccagc tcgtgaactt ctttgatttc
    15901 ttggatgatt tctttgatta ctggtggttt gattccaaat cctgcatccc cagctaaagc
    15961 ttctccactc aattttaaga ctacacgctg atatttcggt gtgaccatgt tcattcctcc
    16021 aatattttct attgccattt taccatactt agatgatttc gacactctaa gactggtttt
    16081 tcttgttttg cttaaaaaaa gggagcgcac aaaccgtgcg ttcccgtctg ttattcaaca
    16141 gaattatttt ttcacttggc tcataacttc ttctacaaag ttatcttcgc gtttttcgat
    16201 accttcgcca acttcgaaac gtacgaatgt tttaacagtt gcgccttttg atgcaacgta
    16261 tttttcaaca gtcatatcag gatctttaac gaatggttgg tccaccaaag cgatttctgc
    16321 tttgaatttc ttcaagcgtc cttctaccat tttttcaacg atattcgctg gtttgccttc
    16381 gttcaatgct tgttctgtaa gaacagtttt ttcatgttct aattcagctt ctggaatttg
    16441 tgattcgttt acataacgag gattgatagc tgctacgtgc atagctacgt ctcttgcaac
    16501 tgtttcgtca gttgttccat ctagaacagt caatacggca atacgtccgc ccatatgcaa
    16561 gtatccacca aatgcagcat tgtcgtcttt ttctacaact tcaaagcgac ggaagctgat
    16621 tttttctccg ataacttgag ttgcttcgat caagtcagat tcaattgtac ctttatctgt
    16681 tttgattttc attgcagctt ccatgtctgc tggtttgttt tcagcaacaa gttctgcaat
    16741 ttcttttact aaatcttgga acatttcgtt tttagaaaca aagtctgttt ctgagtttac
    16801 ttcaacgatt gctgcagtgt tacctttcac agcaactgaa gccaatcctt cagctgcaat
    16861 gcggtcattt ttcttcgcag cctttgccat tcctttttca cgtaaaagat caactgcttt
    16921 ttccatgtcg ccttctactt caactaatgc ttttttcgca tccatcattc ctacgccagt
    16981 catttcgcgt agttctttta ccattttagc tgtaacgtct gccatgttta actcctcctg
    17041 tgtgtttttc tttttaaaaa aagctgcttc aaaggagagt gaggcttgtc gcccagcctt
    17101 tgaaacagct ccatcattca aaattattct gctgaagaat tgtcgccttc aacaacatct
    17161 acgatttctt cgattgatgt tgctgtgttt tcttctgtga aatcttcttc aactacttga
    17221 tcttcacctt ggtttccttc gatgaaagca tcagccattt ttgaagtaat caatttaacg
    17281 gcacgaattg catcgtcgtt tgaagggatt actacgtcga tttcatctgg atcgcagttt
    17341 gtatcaacca tagctacgat tgggatattt aatttgtgtg cttcttgaac agcaatacgt
    17401 tctttacgtg ggtcaacgat gtacattaca tctgggattc ttggcatatc agcgataccg
    17461 cctaagaatt tttcaagacg ttcacgttct ttgttcaaac cagcaacttc ttttttaggt
    17521 agaacttcaa aagttccatc ttcttccatt gcattgattt gtttcaaacg gctgatacgt
    17581 ttttggatag tatcccagtt tgtcaatgtt ccgcctaacc aacgatggtt aacgaagtat
    17641 tgtcctgcac gagttgcttc ttctttgatt gcttcttgtg cttgtttttt tgtacctacg
    17701 aataatgcta cgccgccttc ttcagcaacg ctcttcatgt aatcataagc tgcatctact
    17761 aatttaactg ttttttgcaa gtcaatgatg tagattccgt ttctttctgt gaagatatat
    17821 ttcttcattt ttggattcca gcgacgagtt tggtgaccaa agtgtacgcc ggcttctagt
    17881 aattgtttca tagaaattac tgccatgttt gtttcctccg atttggtttt atttttcctc
    17941 ttcttatctt ctaccttgcc ggcaaacttt ttcaagcacc taccgacaat ccaataagaa
    18001 tgtggatttg ctgatgcata aagcaccctc agctattata cagacaaaag atccgtatcg
    18061 caagagcaaa tatacttttc tgctcaacat tcaatagttt acctgaaaga taaaaagaat
    18121 tcaagaaaaa cagtagattt ctctttgttt ctttattatc ctttttccat aaaataattt
    18181 taggtataaa agacgaatat tattgggcaa tccttctttt ttttgttatg atacatgtga
    18241 aatctttcgt aaaggagaaa gaaaatgcgc aaagctcaaa gacatttgct gattaaacaa
    18301 gtcattgaag aattcccgat ccgtacgcaa gaagaactat taaacaaact tgggcattat
    18361 ggcgttaccg ccacgcaggc aacgatttct cgagatatcc gtgatctaaa aatcgtgaag
    18421 gcacctgatg aaaatggcgt ttcccgcttc gtattgtttc gcgaaaaagg aactcctgag
    18481 actaggactg aggaagagga tcggctggtt caaatgattg aagacattgt attgaaagtc
    18541 gaacgagtcc actttttgac gatcgtcaat accttgccag acaatgctca tttatttgca
    18601 tctgttttag atgagatcaa acctccctct attgtcagta caattgctgg attcgatacg
    18661 atcatggtca tctcggaaaa tgaggaggac gcaaaacgag tagaagaatt tcttcaccat
    18721 ccaactgaag tatcactttt ttaatcagag cacgtattcc taatatggaa tgcgtgtttt
    18781 tttattttca atattttcct tattaaataa acctttacac tctttacaaa aatacatcat
    18841 tttttattca attttttcaa caccttgatt gaatcacaat aatcatcatt tatattggat
    18901 gttaacaaga gcagcttctt gctctttgtg tatttcaaaa aagaaatgaa aggaaatcat
    18961 atgtataaat tattaactcc tggaccattg acgacttctg aaacagtgaa aaatgctatg
    19021 ctgatcgatc attgtacttg ggatcaagaa tacaaagaaa ttacacaatg gataagaaaa
    19081 aaattattgg atcttgctca agtatcggaa ggaatataca caacggtctt aatgcaaggt
    19141 agcggctcct ttggggtaga atcggtatta agttctgtga tcaggccaga agatactgta
    19201 ctgatttgtt caaacggcgc atatggacaa cgaatgatca acatgtgcga atgtctgaaa
    19261 ctgaactata ccgtttatga ggtatcggaa catcaaatcc tccaagctca tgtgattaca
    19321 cagctgattg aagagaatcc ctctatctcc gcactagcaa tggtccatag cgagactact
    19381 tccggtattt tgaataattt ggaagagatt gctgctgtca cacaagaaaa agatcttttg
    19441 tttatagtgg acgcgatgtc aagctttggt gggataccga ttcgtgtagc cgaactaggg
    19501 attgattttt tgatttccag ctcaaacaaa tgtattcaag gtgttccagg cttttctttt
    19561 gtacttgcta ataaagcgga actagaaaag cgaaaaaatg tgagtcgaag tttgtcactt
    19621 gatctatatg accagtaccg cgtgatggaa aacgagaatg gcaaatggcg tttcacttcc
    19681 cctacccata cggttttggc ttttcaacaa gcgctgaagg aactagaggc agaaggagga
    19741 atcgaaactc gatattcacg ttatctcgcc aataatcatg tactacgaca aaaaatgaca
    19801 gagatggggt tccagccttt cattcaagaa tatcaggggc cattcatcac ctcttttctt
    19861 taccctaagg actggcgatt tgattttaat catttttatc agtatttaaa gcaaaacgga
    19921 tttgtgatct atcccgggaa aatcagcgat catgaatgtt ttcgtatcgg aaatattggc
    19981 gagatctatc cggaagatat tttggaactg acagcaatta tcaaagacta taaggagaac
    20041 gttaatgatt aaaactgtaa tttttgattg ggcaggaaca actgtggact ttggctgtat
    20101 ggctcctgtc cacgctttta gaaatgcttt tctagaaaaa ggaatccaac tgactgacaa
    20161 agaaatccgc gaaccgatgg gaaaattaaa atgggatcat atccaacagc ttctttccct
    20221 cccttctgtc aaaaaacaat ggatagctat ttatggacaa ctacctcagg aaaaagaggt
    20281 ggaggaattg tatcaacaat ttgaacatta tctgtttaaa gatttggtca agcattctac
    20341 cttgaaaccc gacacattgg aaacgatcga acggttgaga aacgctggga tcaatattgg
    20401 ttccacgact ggatttactg ctcctatgat gacaatcgta gcagaaaccg caaaaaaaga
    20461 agggtattca ccagacttca tggcaacttc tgaagacgtc gaagggtatg gtcgaccgtt
    20521 tccttatatg atccataaaa acatgcagta cttcaaaaac aagtcggttg atgaagtaat
    20581 caaagtcgga gatacggtct cagatatcca ggaagggaaa aatgccggag tgttgacagt
    20641 aggtgtaatt gaaggtagtt cgctaatggg gatttcttat gatgagtacc aaaaattaga
    20701 gatggggaaa aaagatcgtt taaaacagtc tgttgctctt agattccaac aagctggcgc
    20761 tgatatttgt gtaaacaatt taaaagagtt aacagaatac attttgtacg ctgacaagaa
    20821 tccagaatga cttttataca aaatcagagg tcgtgaaaaa gctgtactgc atcaagtaat
    20881 aagaacagcc aaacaaaaat agctcctcat atttttcgtt tggttaggct tattaccgca
    20941 gatgcaagct tttgaacacc gtttattcga aaaaaaaagg gagctgtgac acgacttttg
    21001 tcacagtccc cttatttaca aacaaacagt gtttaagaga caacctctcc actaaacggt
    21061 gttcattcag ctgactctcg gtatttgctc aaaaaacgat aaaatttgaa aagctatttt
    21121 ctcttttgtt tctctaatac tcggatcaga acgctgattt cacattctct ttttcttaca
    21181 tcttatctgg tgcatgaagt cctagcaaac gaaggtcttc tttcaataat acagtcactg
    21241 cataaactag agctaagcgt gcttcttttt gttcatcatc agctaaaatc ttcgtatgag
    21301 catagtattt gttgaatgct tgtgctaatt tgattgcatg tttcgcaatc actgacggtt
    21361 cgtatttttc cccagcagac aagaccgttt cagggtattt ttgaactaat ttaaccactt
    21421 cccagctggt atcgtcattc aatgcatagt ctgctgtttc tgaagggaca aatcctgctt
    21481 tttccaacag gctgactgca cgagcatgtg tatattgcac ataaggtcca gtttctcctt
    21541 caaatcgaac aacttcttcc aggttaaagt caaatgtatt cagtcgatcg tttttcaaat
    21601 cgtggaaaat gactgcccca accccaactt gtttagctac agcatctttg ttttccaaat
    21661 cagggttttt ctcgctgatt tgttctttag cagattggat tgcttcattt aatacttctt
    21721 ctaataagac gatctttcct ttacgagttg ataatttttt accaccttgc gtgatcaaac
    21781 caaatggaat atggcgcata tcgtcagacc agtcaaagcc catttccttc aagacagctt
    21841 tcaactgttt aaaatgatag ctttgttcat tccctactac atatagagat tgtttgaagt
    21901 cgtaagtacg tttacggtac aatgcagcag ctaagtcacg tgtaatgtat agtgtcgcac
    21961 cgtctgactt cttaatcaac gctggattca ggtcataagc agataggtca acgatctctg
    22021 cacctttatc ctcgttcagc aaatgttttt cttctaaaag tttgacgatt tcatccatct
    22081 tgtcattgta gaaagcttca ccgttcaacg aatcgaaacg tacttccagc agatcataga
    22141 ttttattgaa ttctttcatc gattcatcac ggaaccattg ccataattga atagcctctt
    22201 catcgccttc ttccagacgt ttgaaccaag cacgagcttc ttcatttaat tctggttctg
    22261 tttcagctac ttcatggaat tggacatata aacgaagcaa ttcattgatt ggttctgctt
    22321 taacagcttc ttctgtaccc cattttttgt aagcgacgat cagttttcca aactgtgttc
    22381 cccagtcacc taaatggttg atgcggattg gctgataacc gattttttcc ataatgaaac
    22441 cgatcgaatt accgataact gtcgaacgca aatgtcccat tgagattggt ttggcaatat
    22501 taggagaaga catgtcgatt ggtactgttc cttgattccc gatattgcta tccccataat
    22561 gttctttttc tttcactact gtttgcaaaa cttttttgct gataagttct ttattcatga
    22621 aaaagttcaa gtaaggtccg acaacttcga ttttttcaaa gttggcagaa tcgatttttt
    22681 ctgctaaatc tgcagcgatt tgctgtggcg cttttcggta aacttttgct aatgaaaagg
    22741 ctggaaatgc aacatctccg tgttcagctg attttggatt ttctagtaat tgttcgactt
    22801 gttccagtgt taaatcatct ttgacagcgt catagacagc ttttgcgaca agatctttat
    22861 tattcatctt tattcctcct aaagtgctcc cttgattgtt aaacaaaaaa aatctctagc
    22921 gacggcatac ttagtatgcc atcactagag acgagatttc ccgcggtacc actccaattg
    22981 tcctaaaaaa ggaccagctc catctgatat cggtagacga ccgtctgttt aaaacagaat
    23041 tctccaagtg cgcttcgttg tatcttcttc attcggcttc caccacttcc gaattcgcta
    23101 gccaagaatg atacaagtac tctcttgttc aacaaatctt agtcttatta aactttcttg
    23161 aattatatca gaaaaaaaaa cgaaaggcta gtcgtttctg ataaaaaaac aatccagatg
    23221 gcctaaagga gaagaaatac cacctggata gctataaatc atttcgtaaa tacgaaagac
    23281 ttaacaaatt aatcagctac gataatcgtt ccttcttcag aagctaaaag attctcgata
    23341 ttttctaaag aagtgatgat tgccttagcg tttggttttg cttcaacaaa tgcaatagct
    23401 gcctctactt tcggaagcat gcttcctgga gcaaattgct tttcatcaat gtattgtttc
    23461 atttctgaaa cagtaacagt ttctaatttc ttttgatctg gtttttggta attgacatac
    23521 acattgtcta ctcctgtcaa tacgatcagc aaatccgcat cgatgatttc ggctaatttt
    23581 tctgaagcaa aatctttatc gattacggct tctcgtcctt ctaatccagt agctgtttct
    23641 acaacaggaa tcccaccgcc accaacagag actgtgataa ctccttgatc gaccagcgtt
    23701 tcgatgacac gtgcttcttt aatcgagatg ggtttagggg aagcgactac ttttctccat
    23761 ccacgtccag catcttcttt gaatgtcaca gtggagtcag cattcatttg ttctttggct
    23821 tcttcttccg tgtagaatgg accgactggt ttacttggat ttttgaatga aggatcattt
    23881 tcatccacaa taacttgagt gactaaagaa accacatctt tatcgatccc tttttccttc
    23941 agtacttcgc ccatggcatt ttgtaaccag taaccgatag atccttctgt cattgcaaca
    24001 catgtatcta aaggcattgc cggtgttttc tcagaatcag cagcttgctg ctgaatcaat
    24061 aggttaccga cttgcggacc atttccgtgg gagatgatca attcatctcc ttgttcgata
    24121 aatttcacta aatactttgc tgtttccatt aacgcttctt gttgggcttt tgcacttgca
    24181 tccgtagaca agattgcatt accccctaaa gcgacaacta cttttcgttt ggccatttca
    24241 ttcgtccttt cttcagttct accgaatttt ctagataaca aaacgagcca gcgttttgtg
    24301 attttccctc aatgctgact cgttttttcg tgagatgaat gtggttatgc ttaaacgcga
    24361 gggataaaca agttacctaa tgttgctgcc atgatcgctt tgattgagtg catacgattt
    24421 tctgcttggt caaattgacg agcatatttg ctgcggaaga cttcatctgt tacttccatt
    24481 tcagtgatgc caaagcgttc tttcatttgt tcaccgtaaa cagtgtttgt atcatggaaa
    24541 gctggcaagc agtgcaagaa gatcaaacga tctgtgttgt gtgttttttc taccatttcc
    24601 atattgattt ggtaaggttt caacaatttg atacgttctt cgaatttatc ttcttcaccc
    24661 attgataccc atacatcaga ataaaggacg tctgctccgt ctacaccttt atcaacatcg
    24721 tcggtaatca tcaattgtgc accggatttt tcagcaaagc cttctgccat tttgacgatt
    24781 tcttcttcag gttgtaattc ttttggtgca acgatacgca tattcgctcc taaaattgca
    24841 cctgtgacta ataaagagtt agccatattg tttcgaccat ctccgcagta agcaacagtg
    24901 ataccttcta ctgttccaaa gttttcttgg atagttaaaa agtcagcgat catttgtgtt
    24961 ggatgccatt catcagtcaa gccattccaa actggaacac cagagaattc tgctagttcc
    25021 tctaccattt tttgactgaa tccacggaat tcgattccat caaacatacg acctagtact
    25081 ttagctgtat cttctgtgga ttctttttta cctaattgga tatcatttgc acctagatat
    25141 tcaggatgtg cacctagatc gatcgctgca gtagtaaatg cagaacgagt acgtgtagat
    25201 gttttttcaa ataaaagcgc aatatttttt ccttctagat agtgatgtgg gataccacgt
    25261 tttttcaagt ctttcaagtg ttctgaaaaa tcgatcaaat attgtaattc ttcttttgta
    25321 aagtcttttt ctgctaataa gcttcttcct tggaatactg attctttcat attaaacatt
    25381 ctcctttaga ttgtttttat ttaagattaa agaggaaatc ccgacggcag ctgacagtaa
    25441 gaccgccgtc aggaaacaaa ttacttcaag tcttcacgga caagtggttg gctcatgcag
    25501 cgtggaccac cacggcctcg tgacaattca cttgaattga tttctaacac tttgatgcca
    25561 tagctacgaa gcaattcgtt cgatacataa tttcggttat aagtgactac aacacctgga
    25621 gcgattgcca atgtgtttga tccatcattc cattgttctc gaggtgcaac gatctcatct
    25681 ccattaccag ttgggatcag tacaagatca tctaatccaa gagcagcttt catcgtttcg
    25741 tgtaagttat ctgaatgagt aatcttgata tcatcaccat ctggttcgat tgtaaagaca
    25801 tccatcttac cgtctttact ttgaattgct ggatggatag tgaatttatc gtgatctacc
    25861 attgtaaata cggtatccaa atgcatcatt gcacggttgt ttggaatttt gattgctaac
    25921 actttttcaa atcctgaatt ttttgcgaat aggtggcgtg ctaatttttc taaagctttc
    25981 gcattagttc gttgagaaat cccaacagct actactttat cgcttaagat caattcgtct
    26041 ccgccttcga tatgatctgg attttcacga tccaaccata cttcgattcc tttatttgca
    26101 aatcttggat gatgtttcaa aatatattct gtaaacatcg attcacgacg acgtgcttca
    26161 aaagtcatct tattgactgt catcccattt cccattgatg cggaagggtc gcgtgtaaag
    26221 tacaagtttg gcattgggtc catgtaaaat ggatagtcat cgtctgctga cagatcgtat
    26281 agactgctag aacgtacttc aatatctttt gtacgaaccc cagccatgat tttatctacc
    26341 atttcttgcg tttccattga tagaaggaac tcatgaagac cctctcttac agcattcgat
    26401 gcaatatgag attcatccaa cattttattc aagaattgtt ctttgacatt gcctgcgtca
    26461 atcgcttctg ccgctaattt ttccaagtaa agagtctcga cgccttcatt ttgcaatgtc
    26521 tttgcaaagt tgtcatgttc ttcttgagca atcggaagat atggaatatc atcgaacaac
    26581 agacgatcca taatatctgg tgtcaaattt tccacttctt gtccaggcct tttcagtaaa
    26641 actgtcttta atttccctat ttcagagaaa acgtgaatag gtttatccat gttgtgttgc
    26701 ctccttcttt ttgattacat gtataggata tcctcctttt gtaatcactt tcataatttt
    26761 gtctcattca aaatgagaca ttcctcacat taatattcga taattaaata attaaacgtt
    26821 tttctatttt taagcgtaat ttattcattt ttta
//
LOCUS       DACDRN010000035        25749 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDRN010000035 DACDRN010000000
VERSION     DACDRN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.035"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(451..1761)
                     /locus_tag="IWJ80_001675"
     CDS             complement(451..1761)
                     /locus_tag="IWJ80_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9461391.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="IWJ80_001676"
     CDS             complement(1773..3191)
                     /locus_tag="IWJ80_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9461392.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3395..4963)
                     /locus_tag="IWJ80_001677"
     CDS             complement(3395..4963)
                     /locus_tag="IWJ80_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP9461393.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4967..5377)
                     /locus_tag="IWJ80_001678"
     CDS             complement(4967..5377)
                     /locus_tag="IWJ80_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP9461394.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5374..6297)
                     /locus_tag="IWJ80_001679"
     CDS             complement(5374..6297)
                     /locus_tag="IWJ80_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP9461395.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6294..7172)
                     /locus_tag="IWJ80_001680"
     CDS             complement(6294..7172)
                     /locus_tag="IWJ80_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9461396.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7159..8496)
                     /locus_tag="IWJ80_001681"
     CDS             complement(7159..8496)
                     /locus_tag="IWJ80_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9461397.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8499..9602)
                     /locus_tag="IWJ80_001682"
     CDS             complement(8499..9602)
                     /locus_tag="IWJ80_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9461398.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9676..11184)
                     /locus_tag="IWJ80_001683"
     CDS             complement(9676..11184)
                     /locus_tag="IWJ80_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9461399.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11150..11842)
                     /locus_tag="IWJ80_001684"
     CDS             complement(11150..11842)
                     /locus_tag="IWJ80_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9461400.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11864..12838)
                     /locus_tag="IWJ80_001685"
     CDS             complement(11864..12838)
                     /locus_tag="IWJ80_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9461401.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13138..14010
                     /locus_tag="IWJ80_001686"
     CDS             13138..14010
                     /locus_tag="IWJ80_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9461402.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14468..16666)
                     /locus_tag="IWJ80_001687"
     CDS             complement(14468..16666)
                     /locus_tag="IWJ80_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9461403.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16898..17890
                     /locus_tag="IWJ80_001688"
     CDS             16898..17890
                     /locus_tag="IWJ80_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9461404.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17950..19371
                     /locus_tag="IWJ80_001689"
     CDS             17950..19371
                     /locus_tag="IWJ80_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP9461405.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19364..20863
                     /locus_tag="IWJ80_001690"
     CDS             19364..20863
                     /locus_tag="IWJ80_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP9461406.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20902..22296
                     /gene="uxaC"
                     /locus_tag="IWJ80_001691"
     CDS             20902..22296
                     /gene="uxaC"
                     /locus_tag="IWJ80_001691"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9461407.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22455..23138
                     /locus_tag="IWJ80_001692"
     CDS             22455..23138
                     /locus_tag="IWJ80_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP9461408.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23140..23976
                     /locus_tag="IWJ80_001693"
     CDS             23140..23976
                     /locus_tag="IWJ80_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9461409.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23987..25741
                     /locus_tag="IWJ80_001694"
     CDS             23987..25741
                     /locus_tag="IWJ80_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9461410.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
BASE COUNT         7834 a         5236 c         4510 g         8169 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactat taaattacag atgatctttt ccatcatact
     3421 tgaaaaactc aaaatcagca taacttcctc tttttttatt tagatcatgc actgctatcc
     3481 cgataaaatt tccagtaaac cctcctgaga gtatacacat gtttttttgc ggacctactt
     3541 tcttccattc ttcagttgat acgtctttca aatgaaactg tgctgtctct ttctttacag
     3601 tgactttcaa aaaagtcttc ttttctgtga gtggaatcgt ccaatcttct atttgtgcta
     3661 ctccgttttc tgattctatc atacgtaggc attttccctt gtcctcattc caagtaacat
     3721 aacaataaat ataattggat tcatttaagt acagtaccag tccagccatc tgattaaaat
     3781 tattaggcat gtattcaaat gctgtctccg catcaaaagt aaaatccgtt tgccgaatag
     3841 ctaacaaatg atgttcgaag gtagattgaa gagattcacc ggaaatcaga cgtaaaaaac
     3901 ctggtctaca ttttgtatca caccaatcag catttggcaa aatgcgtctg gcattccagt
     3961 tcttctttag ctctccttca aatgtatcca taaactgatg atctttttct tgttgtacct
     4021 cttcatttgt ctcaatcatt atttcttcca tcggagttgt ctggctatct ttaagtctta
     4081 accaatcgtc ggacgtccaa tacacttctt gaatagcggt ttctcttccc aaaatagccg
     4141 caccgtttaa tggtctggta caaagatatg tcatgtacca tcttcctgac ggggtttgta
     4201 ctaagctccc atgtcctgta cattgcaatg tagaagcagg tttgtcacta gctgtcagca
     4261 taggaaaacc aggatcaagc tcgtaaggac cagtaatatt ctttgctcgg caaactgtaa
     4321 ctgaatgtcc tcttcctgta ccgccttctg ccgtcatcaa ataataatac ccgttacgaa
     4381 aatacaaatg tggtgcctcc gttttcgcta attctgtacc tgcaaatatt ttatagatgg
     4441 gaccatcaag cttcttcttc tctgcatcat attcttgtaa tacgataccg ctggatttat
     4501 tcggtgtgtc cagacgataa tcccatagtt cattgacaag ccattttttt cccgtttcaa
     4561 gatcatggaa caaagaagga tcaaagccgt tactatttaa ataaattggt tcggaccaag
     4621 gacctgtgat ctcagatgca gtgatcaaat aattgtggac atctttaaac ggacggcctg
     4681 cacttttgac atctgtgtaa acaagataaa acaatccatc agcataactg atctgtggcg
     4741 cccatatact tccatttgta ggatttcctt gcaaagatac ttgagtggtc aaaatatccg
     4801 ttgtatgtga ccagttgact aaatttttcg actcatatac tcggattcct ggaagccatt
     4861 caaaagagga gacaataatg taataccgct ctttcactct taatatatta ggatctggat
     4921 tgaatcctct taaaatagga tttttcactt tgatagtctg catgattcag gctccttttc
     4981 tcattcttac tcgatataaa ctgaatgccc cagctgcgaa ataaaaaaat gcaaaaggaa
     5041 acgcaataaa ctgagtgcca atcaacaata tgaatcctgt tataaaatac aaaccagtgg
     5101 cagttgttaa gtattttcct ttagcagtca aaaacaatgc tactgctaaa aaagcaagtg
     5161 aaataaacaa cacaacggca aacagaccag tcatgtcctg taataatcca aatgtatttt
     5221 ctaaagacaa agttgaatga tctacagcaa gcgactgata acttttttta aactctgacg
     5281 cagacatatt acttaaagta actgctaacc cgccaaagaa aatcaaagat agactgccac
     5341 cgaccattcc tagcaatagt tcccattttc ttttcatttc tgaaccccct gcttttctaa
     5401 atgtatccat tcaggtcttt tgatcgatgt ttcataattg ttattacgtt cagagaattt
     5461 gacagtctgt tcatttttct tgtcatccca attatcccaa ccagttgggt gaacatgccc
     5521 acctatctta cacgcttcaa ataagacatt tgcatacgga cgccacggtc ttcctaaata
     5581 gacgtttcta gtatcaggtt ctgcagtcaa ggtacagctt tcaaaacgat accctgtttt
     5641 ttgatttttc ggcgtagatg ctgctgtgat gtatccagga tttttactgg ctaagctatg
     5701 gatatgacac tgatgaaaaa cggcatctgc cccaccaaat atgaaatcca ccgttccttc
     5761 aatccagcaa tattcatagg tctgacgaca atactcagga ttggcagaag ccggtgtaca
     5821 aaaaggagta ccatcttttt gcaaatcagg caacggaccc gtgcataagg tatcctgata
     5881 accagatagt cgacagtttt ttaaagatac gcgatctcct gaattgaata aagcaacagc
     5941 ctgtcctacc ttttctcctg gacctgctgt attttcgatg tgaagatttt ctaatacgac
     6001 attcgatcct tctaagaaaa aagtagcact tcggaaagtc cctaactggc taccgtcaag
     6061 atactcctgt tttgcaaaac gattaccgga gataactact gttcctgctc ccacaagttt
     6121 aaaattggat aatctgcttt ctatatattc atcatatctt ccttctttta ctatcaagac
     6181 ctttggatgg ctatccggca ggcctgccaa ataatcgatt ccttcttgga tagtttcaaa
     6241 atccgtcggg gtatctatag cattctttcc aattatcagt ttgtctttca tcctcataaa
     6301 tgctccctta tgaattgttt ccaaatattt ctcatttctt gtgtttccat atgaccgcct
     6361 gttaaagaaa aagaagaaaa gttttctgga ctacccattg attgatactt tttgtccagc
     6421 ccttttctca gcttcagtac gccttcgtcc aagcacatcg tatcgttttt tccaactaag
     6481 ctaagacgag ggcgcggagc gattttttct tggattgcta acgttgtata agctgtcaat
     6541 agatttggca catagtaata aaacccatga tgatctaggc ggcgatgtag caaaagtatt
     6601 tcgatgtcca cctgtgccgc tagatcaata caaactttga cacgagaatc caatgctgtc
     6661 acccaccagc ttaataagcc tcccatagaa agaccaatcg ttgcgagccg ttgagtatct
     6721 gtgtcttccc ttgtctccaa ataatcgatc aagctgatca catcataaat acgcattccc
     6781 cataacgttt ttcctgttaa taaaaattgt ttgaacaact cgctttcaga aatacctccg
     6841 cgttcttcaa atccccaagc atcaatcgac caaacagcat atcccattcc tgtcagtact
     6901 tctgcaaaag atggagaagc taagtaagga gcaccatgga tcaattcact tttcccgact
     6961 gaaaaatctc cgccgtgaga atgcaggtaa aggaccggcg gaaacggtcc ttctttttct
     7021 ggataagcat agagggctgg tactgtttcc aattcattta agtacaattg atagtattca
     7081 agcataaaac cgttttgttt ttctcggtat aatcgttttc cactaggggt atgtctgcta
     7141 gatggagcac ctaaaaaatc attcaactgc ataagcaaag tttttacgaa caaccagttc
     7201 atccatattt tctgattgat ttgattggaa tttctcaata ctgatttctt tagtattttc
     7261 taatagaaat gctggtccct cgtgattttc aatcgacatc tggttaaagg aaaccttacc
     7321 cgcaaagcca atataaaatc cttgtctgct catttcctct attcctgtca tcatagcagg
     7381 aacaccggga actgcatctt gggccatcga gacatcgata ttgtcaaatg tgatttcttg
     7441 tatgaattgt tccgctaatc cataaataaa acctgcagaa gcatggacat tccttgccgt
     7501 gatattcgaa aaatggatcc ttctgaatgc tggtgtccgt tcatctatcg gataagcctt
     7561 tttttcccat acatacggtt ctttccctcg cggaccacag aaataataaa gattcagtat
     7621 aaatggacac atcacattat ccatgactat attggatact cgaatatctt caacgatccc
     7681 gcctcgacct cgtctagatt tcaatcgtat tcctcgatca gtttcttgga agatacaatt
     7741 tgaaatggta atattacgaa tactaccact catttcactt cctaatacga caccgccatg
     7801 tccatggacc atcgtgcaat ttgtgatcgt gatattttcg caagcgatcc tttcgtacgt
     7861 atcttcagtc cctgccttga ttgcaataca gtcatctccc acgtcaatat ggcaattact
     7921 aatacgcaca tttttgcatg attctggatc aattccgtct gtatttggtg aatccgctgg
     7981 gttcaagatc gtcaagttat caaacgttgc attactgcaa agaatcgggt tgatcgtcca
     8041 acttggtgac tggatcaatt taatatcttt cacagtaatt cgatgacagt tatggaaact
     8101 catcaattta ggacgaggat aagcgagatt atctggttca tttctaaaag tatgccacca
     8161 tttttttcca tttccatcta atgtaccgaa accagtgaca gagatgttct ctacattttg
     8221 agcatagata caggaagcat aaacttttcg atggactcct tcccaacgtg aatgaacaac
     8281 aggataatct tttggatcgt cagagaattt cagaaccgct cctgcgctta aatgaagttc
     8341 gacattgctt tttaaaaata aagctcctgt tagaaattca cctgctggaa caactacggt
     8401 cccgcctccg tcacttgctg cagcatcaat ggcttgttgt attgcttcag tattcaattc
     8461 atcaattgat gcgccaaatg tcaaaatatc atacatattt ataatgcctc cacttcaatc
     8521 aatgcttgca agaatgcacc aacgcctttt tgatcattac aaataattgg ttcactgatg
     8581 tagtaagcat atgaaccatc tcgctgatct gctccgccta accctgctac ttgacagttt
     8641 ttattcaagt tcagccagcc ttcttttgtt tccagaacaa attcatcaag taatccttga
     8701 tgtgatttct tcacttgtgg aagccattct tctttgttca gcagtcccaa acgtattcct
     8761 ttagccattg cgtaaacaaa catactactg ccggaagctt ccagataatt tcctttccgt
     8821 tttgtttctg ttgttacttg ataccatgtc ccgctgtttt cttcttgata tggaagtaat
     8881 gcagccagtg tcttttctag aatttctttc agcaaagaac aatctgtttc ttcatgaagt
     8941 aattcatagg tatctgctgc agccatcaaa taccagccaa tcgatcggct ccaaaaatga
     9001 tgggatagac ctgtttttgg atcagcccat ggttggacct tcttttcatc ccatgcatga
     9061 atcagcaaat ttgtttttgg atcgaataga tgtttataac tgatttcaaa ctgtctcaaa
     9121 acatcttggt aatctttttc ttcactaaaa ttctggataa attcagcata aaaaggagaa
     9181 cccatatata agccatccaa ccaaatttga taaggataaa tttctttatg ccaaaaagct
     9241 ccttcggatg tgcgtggatg gtttgccaac tgctgggaaa ggagttctgc tgctttttta
     9301 tatttttctt cgcctgtttc tttgtacaat aaaaagaaaa gctttcctgt atttacatgg
     9361 tcgatattgt attcttcaac gctgtagcca cggatcgttc catcttcttg cacgaaatag
     9421 tcgatattgt cttttatgaa atcaaaataa attttttctt gggttcgttc ccaaagtaat
     9481 tggaaccctt taagtacgac gccatagtca taagaccatt ttcctttata ccacttttct
     9541 tcatataagc gaggtgttct ttccatgata ctttttgcta atttctttga ccagttttcc
     9601 attatttgct ctcctttaaa aattaaaata ggatttggga caaataccaa gaagtatttg
     9661 tcccaatcgt ctatattact gtgccgctgc tttgtatgct tcctcgtact cttgtgtgat
     9721 tcgatcgcct ccttgtgctt tccagtcttc tacagctttt ttgaatccat cttcgtcaag
     9781 ctgtcccatg atgtattgca ccgtagcatc aatgacgatt ttttcaattt ctgatcctac
     9841 ttcattgttc gtcggtgaat ccaatggcac agaaggatcc attacagcaa attcttcatt
     9901 ttctttgatc aattgattag agaattcttt ttcagggtct gtatctttga aagagaacgt
     9961 cacttcacta ggacgagagc ttgataacgg ttgcacctct tgttgccaaa gatccatatc
    10021 gataaattcg atagaaccat catcattcaa ttcataatgc gttccttcga taccgccagt
    10081 catcaattta tacacatctt cttctaaaag atcatcaaca aattgaagta atttcttcaa
    10141 ttgtgcttcg tcttttactt gagatttggg aaatgctagc aagccgccaa taccatttcc
    10201 ttctgaccaa acatgataac cgccgccttc accatctgag atcttgttta caggcactaa
    10261 ttccatatca tcttgaatac cttgacccat cgtcttcaga ttcttgatat ccaccattcc
    10321 agtgtagatt ccagttcttc cttgtgcaaa ttgttcttgc tgatcagtct tttgggtaac
    10381 tgcaaagtct tgtgccaaat agccattctc aaatagatct cttgaaaaat tcaaagcttt
    10441 catatactct tctgtatcaa attctggagt gaatttgcca ttatcgtcta cagcccagcc
    10501 atttggtgct ccaaaataag aagtgaaaag tttaaagctg gtataacgaa tatcactagg
    10561 cgcacgatcg ccaaaaccaa ctgtgtcgtc cacaccattt ccatcaggat cgttttctgt
    10621 aaattgttta gctacttcat aaagctcatc catggtagtt ggaacatcta gtcctagatt
    10681 atccaaccag tcttttcgaa tgactaagcc agcacgagcg tagtcttttt gtatcggtac
    10741 gccgtacaag gcaccgtcga tactcgcagc ttcgattcta tcttcagaaa tttgacttaa
    10801 atttttatag tcttttaaat attctgatac atcccagaat acgcctgatt ttaacgcatt
    10861 gcggacagaa gagtttttca acatcgcaat ttgaagtgta acgatatctc ctagttcatc
    10921 agaagccaat gcagttgtta atctttcttc ttttgaagca tcaggaatcc agttgaatgt
    10981 cagttccgta tcggtttttt cttcgatcaa gttcacaatc gtgtctgtag gcggtgaagc
    11041 atgatgcaaa atatttaacc aagtgatttt agcttttcca tcttctgtct ctgaagaact
    11101 gttacttgaa ctgctgccgc atgcggcaag tgctagaatt cctgcaaaac taagacctaa
    11161 ttttacgagc gttgcttttt tcataaattt tctccttcac tgttttaata atatttttgt
    11221 tttcttctgt tttttctgta tatgagttct tccattccaa atggcaaaat atagaatttg
    11281 ctgtccacgt gactgcggaa aagaagagac tgccaccgat aaaggttaaa acacctggca
    11341 ttgtttggat gatcatcaga taaatcccta ccatacttaa tatcaacact aatgagtaat
    11401 gaagatgtga gaatccgaat aaaaatgcca ttttcatttt gaaaaaatat cctttccaat
    11461 cataatgagc catgattggg aaaatataga gtgctgagag cacatatatg aacgcagcaa
    11521 tatacaaagc gatcctaaga taccaattgt gtatgaaaga aagatccaca agtaaaacaa
    11581 tcccgatggc gccatatatc cagctcatca gcaaggtttc acgatagttt tctttaaaat
    11641 atgtgaagta cgttttcgtc aaaggaaacg tttcttttga gcgcagccat tttctaatca
    11701 ctgcaaccat cgcatacgat gcaggaccga aagtaaaaac tcccaaacct gcgatgacta
    11761 aaccgcccca cagaaaattc aaataaacca actgtaaaat acgatttagc cttccgttta
    11821 ttttatctaa tgactgcagc atatctttcc ctctctttct atcttagtaa acgccgtctt
    11881 ctcctagctt tttggcaatc ttattagcta aaattaccat aaacaatcct acgacccctt
    11941 taaacaatcc cattgctgta ctgaaactta actgtccatt cttcaatcca gctgtataaa
    12001 tataggtgtc gaaaatttct gcaacgcttc gattcaatga attcaataac aagtacatat
    12061 gctcgaaccc taagtctaat gtctgaccga tttttagaat cagcaaggta atgattactg
    12121 gacgaattgc cggcaatgta acatgccatg ttttgcgtaa gcgtcctgct ccatccattt
    12181 ctgcagcttc atacaactgt gtatcgactg cagttatcgc tgaaaggtag atgatcgtag
    12241 accagcccaa ctctttccag atcacttgtc cgatatatac tgtgcgtgtc cattcagggg
    12301 atgttagaaa actgattttt tcaaatccca ttgttgcaag gaattcattc aatacccctc
    12361 cgtcaacatt caaaaataca taagtaatcg aaacaatgat tacccaagac atgaagtgag
    12421 ggatgtaaat gatcgtttga atcccgtttt tcaatcgttt gttcgttact tcatttaata
    12481 gtaaagacaa aatgattggc atcggaaaaa acacgaagat atttaatccg aacaagatca
    12541 acgtatttct taataatgta aagaatgtcg gttctgtaaa caaacgaatg aaatgtttga
    12601 aaccgaccca aggacttccc attattccta aaaatggttg ataatcttga aaagcaataa
    12661 cgagtccccc cataggaaga tacctaaaaa tcaagaaata aaggactccc ggcagaatca
    12721 tcacatataa aaatttattc gttttgattc ctgcaattat tttttttcgt cttctgagct
    12781 tttcttcttt tttcgatagc gtcagttgaa atggttcttg tttctttaca ctttccataa
    12841 gcccctcctt agttttgtga atgtttgctg cttatgactt catgatagat aactatctga
    12901 attacgtata taatcatttt taccgtaagt aaaaacgctt acatagcaat gatttttgtg
    12961 ctatacaaaa cgataaagtg tattttttca caaataaact acgcatagca aaccttctat
    13021 ttttttctat ctgacactta gcataggaaa tgattatata cgtaatcttt caccgatgat
    13081 aaattgaaca tatagaaaca gaaacattta atttattttg aggaggagcc tatctatatg
    13141 aaaaagaaag tctctatagg agaaagaagt tttacacttt tcaacaatac gtttctaatt
    13201 ttgctagctt tgatctgtat cgttcctttt ttaaatatca tcgctacatc ttttgcttct
    13261 acacaagaag tcgtcgcgaa aaagtttatc ctttttccta ctaccttttc tttagacgcg
    13321 tatcgctaca ttctttctac gccgaccatt tttagagcac ttgctgtttc gattggtgtt
    13381 acaggtgtcg gaacgatcgt cagtatgtgt gcaacttcac tgatggccta cggtctttcc
    13441 agaaagtatt tgtttggtcg aggtttcgtc aactttcttg ttgtattttc tatgttgttc
    13501 agcgggggga tgattccaac gttcttagtt gttcgttctc tagggctggt caattcttac
    13561 tgggccatga tcctgcctgt ggctgttaat gcaatgaata tgatcattat gagaaacttt
    13621 ttccaagcct tgccggatag tttggaagaa tcagcaaaaa tggatggttg cacagatttt
    13681 ggtgtatttt tcaaaattat gctgccatta gcgttaccgt ctattgctac tatctcgctt
    13741 ttttatgctg ttacttactg gaacacttat atgactgcta ttttgtatat caacgattca
    13801 tctaaatggc cgatacagat tcttttacgc caaatcgtca ttgtatcaag tgggatgcaa
    13861 gcagaaagta gtgccgttga tgtgattcca ccagcacaaa cagttaaaat ggctgttatc
    13921 gttattgcaa cagtgcctat gctgattgtt tatccttttg ttcagaaata ttttgtcaaa
    13981 ggagcactcg taggttctgt caaagggtga ttatgtactg aacgcaaaat ggtagttgtg
    14041 tcagaagtgg tcagcttcaa gaaataagaa ggcatttacg aaaattgctt cttaaatttt
    14101 tgtgaaattt cggcttattt ttcgaagaat ctactcctgg aacaccgtgt atcagaggtt
    14161 gtgaaagaga cgttcagcct tgagcgatga taaaaactct cacaaacatt gcgacgagta
    14221 acccacagga gcaataagaa gcgattttga aaaatagctt cccatatttc acaaaattat
    14281 gccttattgt cgatagcctg tctcttgaac gccatttatt cgtaaaaaag aactgtgaca
    14341 agacttttgt cacagttctt tttgcgtgct tagccttgaa atttaactat ccataaatac
    14401 aaatactagg gttattctaa cttcttttat tgcttgaatc agtccacaag aaaaacagcc
    14461 tcttgattca caaatatttt tttcgatatt ctcccggcgt aatcgattcc ttctttttaa
    14521 agaaacgaat aaaattttgc ggatttcgat attgcaatct ttctgcaatc tcttttacgg
    14581 agaggttagt ctctttcaac catttttttg ctacctctaa acgatgattt tgaacaaaat
    14641 caccaaattt ttctcctgtt tcctttttaa atacattact taaatagtta gggttataat
    14701 gcaacctatc agcgatcaca tctaaagaca gatcacgatc atattctgtg tagacgatat
    14761 gcataatatt ctccgacaaa tttttaaatt catgatcagt gattgcttga gagcattctg
    14821 tgattggcag aactaattgt tctaaaacaa gtttttctag ttctttggga tgataattgt
    14881 tcagtgcttt tacataaatt tgtttcatgt tatcaaagtt ttttgaatct actcccaata
    14941 actgtcctaa ttgaacgagt tcattgatca agcggaccga aactacttcc cgactcaaag
    15001 gatttttatt ttgtgcaaac aatgcatcaa tcaattcatg tactaaattc ttcacttcct
    15061 gatttccaga acgaatggct tcaaacaatc gattttgttc ttctatcgga tatctaataa
    15121 gtgttttttc atgagcagca ggaacgattt cctgataaaa aataattgaa cttggaccag
    15181 tatttacacg ataatacagg gcttcttttg cccgatcaac cgattgcttg ctttcattca
    15241 gcgtttcgaa ccggttactg atgcctatac tgatagttaa attcaagtat tcttttacac
    15301 gtttttgaat gagtcggcaa tactccatga cttttttgct tgcttctgca tcatctttat
    15361 ccattcgata aatagtggct tgcatttctt cattcaaaac gatcggcagc attcgatttt
    15421 cctttggcac aatttccgtg atcatgttgt tgatcgctaa aagtagcaaa tcacgctccc
    15481 caccgtgact atcttctaac agatcgattt gaaccaaagc tgtataatag cagtcatttt
    15541 gagaactata accaaattgc tgcaaccgct gatctagttc tcgcctttct actcgatttc
    15601 taaataaact aagtacaaat agcgtttcca gctgtggctt ttgggtaact aagtgtgcac
    15661 ttaacgcttc tttttctcct atgatccgac tgactgattg agcgaccaaa tctagttctt
    15721 tcccactgaa tccttttgat ttaagattca gacgttcctg tatttgagaa atcggacggg
    15781 caaatctgtc cgaaaataag taggaaagaa gaccaatgag tcctaccagt gttaaagaca
    15841 ctgcaactag cccgattctc agattgcgga tcattgcgcc aattgcacta gagtcaactt
    15901 ctaatctgta aatccaatta ttataatctg atttcagtac tatatacgtt ttatcttttt
    15961 ctcttatcac tttcagctga ttgtaatcta gatccacgtc cgttaataca gttggttgct
    16021 ctttattttt tactgtctga gtagtaaaaa ggttgccaac ttcgttttca atagaaagta
    16081 tgtcttctcc actttcacca acaacttgtt ctattgtatg tttctctatc gtagcaattc
    16141 ccaatgcata tttttctttt tcaaacattg gcaaagaaat caccatttcg attcctttgt
    16201 ttttcttttg ccaaaataaa ttatttttgt tatttatgta cttctgttga tacttttcaa
    16261 cttcctcttc ggtcagttga ctcaacgatc cattgattac tccccattta ccagccaaac
    16321 tgactaaatc ataattatta tcatccataa caatcgtttc tataaaattt agttctttcc
    16381 tgatttcaga ataagtggag taatctgtat atgtcagcgg atgcttgaat atgtccgaga
    16441 aatttttggt cgaaccatac gtagaaaacg aatattcaat cgttctaatt ttccattcaa
    16501 cgttagtttt aacttgcctg agcaaattct ttttgtctgt taaataactc gattcgatcg
    16561 attcataggt attctggtaa atgaataaac taaatcctat gacaagtacg gctcctaata
    16621 aaaacatcgg taaaaatatt ttataaaata ctgtactttt cttcattttc ttcctctcct
    16681 ccttttaaaa taaaaggctt tcatttttct gtaaaattga tactcatcta attaaatgag
    16741 cattccccgc tttcaagaat agcataatta aaaataaaaa tccaagtaat ttttaacgtg
    16801 tgcaatcttt tcatatcaaa gatctattga cttatcgtag ttatcgtaat atgatgaatg
    16861 cgttaacaaa aagtgaaaaa gtaaggaaaa tgatggaatg gtaacgatca aagatatcgc
    16921 taaagcagct ggcgtttctc atacgacagt ttctcgtgct ttgaataaca gcagtttaat
    16981 aaaaaatgaa acaaaacaaa aaatacgaca actagctaag gaaatgaatt attcgccaaa
    17041 ctatagtgca aaaggtttag tgaatcaaaa aaaatatcta atcggactat ttttctcaag
    17101 tttgcataaa ggaacttcat caaatttcct agcagattcc attgcaggca tccattcagt
    17161 attagacaca aattacagtt tatctgttga aagtatcgat gttgttgatt taacagaaat
    17221 caacctgcaa cgctacgatg gactgatcgt tatgagtcaa agtgatgatg atcagccttt
    17281 catcgactat ctcaaagaaa aagaatttcc tttcgttgtt gtcaatcgtc acatcaatga
    17341 tccagcggtt atcaacgtca ctgccgatga tgcggccggt gttatgcagg cagtagatta
    17401 cggtattgga tgcggacata cgaagatcgc ttatattggc ggaaagaaaa attttcgttc
    17461 gacaaaagaa cgggaaaaag gggtattgaa aagtctaaaa aaagcgagca tccctgtcaa
    17521 tccagcctat tttttaacag gagattacag cttagaaagc ggcctccaaa acatggaata
    17581 cctattatca ctttctgacc tgcctactct ggttttttgc ggcaacgacg acatagcgat
    17641 tggtgcattg agagctgtac atttagctgg cctccgtgtt ccccagaaca tttctctgat
    17701 cgggttcgat gattctccag tcgtctccta tctcaatccg ccgcttacca ccgtacgtaa
    17761 gcctttgaaa gaaatgtgtc aagaaggaac aaagctttta ctagatttga tcgaaggcag
    17821 gacgatcgaa caatctaaaa ttgagctctc caccttgttg atccttcgtg agtcgatcaa
    17881 ttacttgtaa actacaagta attttatata aaaatgttaa cgtgtgcata acacagaaag
    17941 gaagtttata tgaaaaaact gacaaaagaa ttagtgaaaa aaaacgaagc acctgtaaaa
    18001 gttcttcagt ttggtgaagg caattttatg cgtggattta ttgattggca gattcagcag
    18061 ctgaataacc aacaattgtt taaaggtaat gttgcaatca ttcaaccttt agaacaaggt
    18121 cttggaaata tgatgaacca gcaagataat ttatatacgg tcattttaca aggactcagc
    18181 aatggcgaaa tcgttgatac ttccgagatt atcaccgtag tagaacaaat gatcaatcct
    18241 tatgatgaat gggatgctta tctatctcta gcagaaaatg acgacttggc ctatatcgtc
    18301 tccaatacaa cagaggcagg aatccaattc gtggagactg ataatttaac agatactccc
    18361 ccacagagct tcccgggcaa actgacagcc tttttatacc gacgtttcaa attaaacaaa
    18421 gctggcttta ccatcattcc ttgcgaactg attgacagaa acggcgaacg tctaaaagaa
    18481 atcattttac gctacgcaga aaaatgggat ttagaacctg cttttgttga gtggttagaa
    18541 aaagaaaatg ttttctgttg tagtttagtt gatcgcatcg tcccaggtta tcctcgcgaa
    18601 gatgcaggtg ttttcaatca acggcataac tatgaagatc aattgatggt caaagcagaa
    18661 ccattcatgc tttgggtgat cgaagggcct aaagaattgg agaatcaact accattcaaa
    18721 caggctggct tgaatgtcat catcacagat gatatgactc cttatcgtga acgtaaggtg
    18781 catttgctta atggaccgca tacagcgatg gtccctcttg ctttgttggc ggaattggaa
    18841 actgtagaag aagtaatgaa agatccttta ttctccgcat atgttgatca gttattcaat
    18901 ctagaattga ttcctatgct ttctttacct aaagatgaat tagccattta tgctgaccaa
    18961 atcaaagaac gtttcttgaa tccttttgcc catcacaaat tagaagcgat ttcattaaat
    19021 agtgtttcaa agttcagcac ccgtctgctt ccagtgttca agaagtatat cgaagaacaa
    19081 aatcaggtac caccgttaat cactgtttca ctagctgcgc tacttcttat gtatcgaggc
    19141 gatcaagtaa agccacatga tgatgaaaag acgataagcg aatttactga tgcttggtct
    19201 gataatggaa cagctattcc tcgattattg caaaacgctg cgctttgggg agaagatttg
    19261 tctcaaatac cgaatgtgac agatacagtt caagaaatag ctgcaaagat cgatcaaaaa
    19321 ggtgtccggt cagtattaca agaaatgaaa ggaacgatgg tccatgcttg aaaccctgca
    19381 accaacaatg aaattaaatc gacgagacag tgtggtcgtc gcattaaccc ctattccaaa
    19441 acaaacacaa ctgacgattg atcagcaaac gatcaccact cttgaagata tcccgcaagg
    19501 tcataagatt gcattgggtg atttgaaagc tggagacaac gtgatcaaat atggctatcc
    19561 aatcggtcat gtgacgacag acgtaactgc cggtcaatgg ctgcacactc ataatgtaaa
    19621 aaccaatctt tctggtgaat tggattatcg atatgaaaaa gatgtccatc caagtcatta
    19681 cctttttgag aaccgaacat ttcaaggtta cttgcgaaaa aatggaaaag tagggattcg
    19741 taatgatttg tttttagtac ctaccgttgg gtgtgtgaat ggcattgctg aactgatcgt
    19801 caaacagttc aaggaaaaac atccagatct gggacagttt gaccatatta cgattttaaa
    19861 gcatccttat ggctgttcgc agcttggaaa agatcatcaa aatactcgag aaattttagc
    19921 ggacgcagta aatcatccca atgccggagg agttttagta tttggattag gttgtgagaa
    19981 caatacagtt cctgaattca aaaaaatttt aggtgaatat gatgaagaac gagtgaaatt
    20041 tctagtggct caagatgttt acgatgagat cgaacaaggt gtggctctat tagaagagct
    20101 tttaacagcg gcggaaaatg accagcgcat atcagtcccg ctctctaagt tgaatgtcgg
    20161 tttgaaatgc ggcggttctg atggtctatc gggaataact gctaatcctt tacttggtgc
    20221 attttctgac tacttgattg ctcaaggagg cagcacgatc ttaacagaag tgccagaaat
    20281 gtttggcgca gaacaagtat tgatggcacg agcggaaaat gaagaagttt ttgactcaat
    20341 cgttgacttg atcaatgact tcaaacagta tttcttgtct tacggggaac cagtttacga
    20401 aaacccatct cctggtaata aagcaggtgg gatcactaca ttagaagata agtctttagg
    20461 ctgcacgcaa aaatcaggaa gctctcctgt cgttgatgtg ctacaatatg gcgaaaaaat
    20521 cagaaaacca gggcttagtt tattgcaagc tcccgggaat gatcttgtag ctgcctctgc
    20581 tcttgcttca tccgattgcc agttggtctt gtttaccaca ggaagaggga ctccgtttgg
    20641 ctcatatgtc ccaacggtca aggtatctac gaatacaact ctttttgaac gcaaaggtca
    20701 ttggatggat tttaatgcag gcgtcttatt agaacaacca atggaagtaa tcttagaatc
    20761 gtttgttcaa aaaatcatcg ctgtagctag tggagaagaa acgaaaaatg aacaaaatga
    20821 cgttcgagaa attgccattt ttaaaaacgg agtaacactt tgattttcat aaaacagtat
    20881 aaaaagaaag aaggaagatt tatgttttta tctgatgatt ttttattaaa agacgattgg
    20941 gcgaaaaagc tttaccattc ctacgcaaaa aacatgccca ttattgatta tcactgtcac
    21001 ttagatccaa aagaaattta cgaaaacaac aatttcagca atctgacaga agcttggtta
    21061 ggaggcgatc attacaaatg gcgcttgatg cgagcttgcg gcgtgcctga agaaaaaatc
    21121 acaggaaatg cttctgattt tgaaaaattt ttagcttggt gtcaaaccgt accaaaaatt
    21181 atcggaaatc cgctttacag ttggtcacat cttgaattga agcgtttctt cggtattgac
    21241 ttgttgataa atgaagaaaa tgcagagaaa atttggacac aagcaaatga aattttggca
    21301 gctcctgatt ttcgccgtcg ggaaatcgca aaaaaatcga atgtagaagt gatttgtaca
    21361 acagatgatc ccgcagacga cttgcactat catcagttgc ttgctcatga agaaccagat
    21421 ttgaaggttc taccttcatt ccgtccagat aaagcactca atattgaaaa agaaggattt
    21481 aatgcatggg tcacactact ggaagaagca gctgaccaaa cgatcaccac ctatcaagaa
    21541 ctgatagaag tcttagggca gcggatagaa tacttccatc aacatggatg tcgtgtttcc
    21601 gaccacgctt tagacatctt acgctataaa gaagcggatg aaacagtatt agaagagatt
    21661 tttcaaaaag cacgagctaa caaactatta tcttcagatg aaatcgatgc ttatcgtaca
    21721 gaaacactga tacatttgac ccacttctat cattctcata attggacgat gcagctgcac
    21781 atccatgctt atcgtaactg taatacacaa atgttggaaa aattagggcc cgataccggc
    21841 tatgatggaa tgaatgatct ttctttgact attcctctgc aaaaattatt gaaccgtgca
    21901 gaagaaacgg atcagttgcc aaagacgatc ctttattcat tgaatccgaa tgactatcct
    21961 gcgattttag ctttgatggg ctgtttccaa aaagaacaga atggaaaact gcagttaggt
    22021 tctggatggt ggtacaacga tactcgagct ggtatgcgcg atcaattgac acaatttgct
    22081 gatggtagtg ctttggggaa ttttgtcggc atgctgacag attcaagaag tttcctgtcc
    22141 tacactagac atgaatattt ccgtcgtgtt ctttgtgaat ttttaggcga aatggtcatg
    22201 cgaggagaag cacctgaaga tgaacaactt ttaggaagtc tagttcaagc gattagttat
    22261 acaaatgcta aaaattactt tggctttttt gcctaattga ttaatagtaa cagtaacgaa
    22321 tcaaaaacag gatagtgaaa taacggaatc cgtttatcca cgttttagag aagaagacgg
    22381 accaaatttg ctccgtcttc ttctcttgct gttctgcttc attgcctata aacctttatg
    22441 aaagaagaga taacatgcct actatccata ttgccggtga ttctactgcg gcagaaaaag
    22501 atgaaaacaa gcgtcccgag acaggttggg gagaaaaaat ctctgcttat ttttcacccg
    22561 agatccagat caataaccaa gcaaagaatg gacgcagcac caaatcattt atccaggaag
    22621 gaaggttagc ccgtctagct tcacagtttc agccgaatga tttcttattt gtacaatttg
    22681 gacataacga ccagaaaatc caagaagaaa gaggtacgct tcccttcggt agctatttaa
    22741 aaaatctgca gatatttttt gaatgcgcaa aaaaagcgaa cgtccaaatg gtcttactga
    22801 catctgtgac gcgacgagat tacttagaaa acggaacatt gaatccagat atacttggag
    22861 actatccaaa agcgatgcgt gcatttgctg agaaacacca tattcctctt ttagatgtgt
    22921 tctcaagatc acaagaattg ttccagacct tctcaaaaga agagacaaaa aaattctacc
    22981 tgcatcttat acctgatact tgtaaaaatt acccagaagg actaaaagac aatacccact
    23041 ttagtccaga aggcgctgat aaagttgccc gaataattgt tgaattgata aaagaatccc
    23101 gtctgcctct tgctaattat ttacaaaaag gagtttgaga tgtttccatt aaatttaggc
    23161 atccgtgccc acgatttaga tgttcgaaat cgtgaagaaa tcgttcaaaa agtatcacag
    23221 cttcacttgt cccatatcca atttgcacca caaaaagcat ttcctgaaca acttaaatta
    23281 actgacatga cacttgggag tgctgcctat tttggtgaat acttccaaaa aaacaatatc
    23341 gaactttcca ttttaggatg ctatatcaac ctatccagca aaaatcaaga agttcgtgca
    23401 caagcattgg caactttcaa acagcaaatt gctctgtgct ctgcctatca agccaagatg
    23461 attgctacgg aaaccggtag tgtctctgtc ggttacacaa aagaaaattt tactgaagaa
    23521 gcttatcaga tcgccagaaa ttctatcatt gaattagtcg ctcatgcaga aaattttggc
    23581 gtaactgttg ctgttgaagc agggatcaat catccaatct ataactatca gttagctaaa
    23641 cgtttaatcg atgaagtagc ttcaccaaat ttaaaaatca ttttagattg tgcgaatttg
    23701 atgcataaag aaaatcatgc tgatcaggaa aaagtgatcc gaggtgcctt ggacaattta
    23761 catggttata ttacagcgct tcatctcaaa gactatatca tggaaaatgg aaagatccgc
    23821 atggtacctg ttggcaaggg ctgtttagat ttccgcccgg tactgcatta tatcaaatat
    23881 gagcgtcctc taatgtacgc cactcttgaa gctactcctg agatttatgt tcctgaagca
    23941 atagaacact tgcagtttat ttaccaagat tgttaaggag aagattatgg aggaacgaat
    24001 acagaaaagt tatcaaaaat atcatgcaaa tgcttttcag acattgctgc atctttatca
    24061 gggaaattat tggcgttttg ccatgtcagg actctttttt attatcaaac atcttccatc
    24121 ttggttaatg ccgatcgcta ttgccaatgt aatcaatgca gcttcatcga aagattcttc
    24181 tcagttgcag gccatttatt tcaacgcttt tttgatggct tgtctgattg gtcaaaatat
    24241 attgacgaat tattttcatg taaaatacca tagcacttcg atccgtcaag tagaagctgg
    24301 actaagagcg acccttatca agaaaatcca agaactatct attccttatc aaaaagaatt
    24361 gcaatctgga cgaatccagt caaagatcat ccgtgatgta gaagctatcc aaacgttgtc
    24421 ctctcaagta tttgtcagcg gattaaatat cgctgtcaat ttattcgtag ctttaggtat
    24481 cacgctttat aaaagtcctg ttgttttcat gtttttctta ttgactgttc ctctcgccgc
    24541 attcgttact ctctttttcc gtaaaaggat cagacagtcc aactcagact tcaggcaaga
    24601 aatggaagag acttctgcaa aagtgatcga agtgatcgaa ttagcaccga ttgctcgagc
    24661 tcatgggtta gaaaaacacg aaacaactcg cttaaataag cattttactc aaatctatga
    24721 aaaaggattc cacttagata tcctgcaatc tgttttcgga tcagtcagct gggctagttt
    24781 tcagatattt cagctgattt gtttgatttt cactgggata ttagctttac aagggcgcat
    24841 ccaacccggc gatgtcgtga tgtatcagac gtatttcacg acgatcgtca attccgtttc
    24901 tggaatgatc acgctgattc ctacgatttc aaaaggcatg gaatctatca cttctattgg
    24961 cgaactgttg attgctggcg atgtagaaac ttatcacggt aagaaaaaaa tccaacattt
    25021 ggatggaaat tttcgttttg aggatgtttc ttttctttat ccggactcac tcaaaccgat
    25081 tatcgatcat ttttctctag aggtgaaagc tggagaaaca attgcttttg ttgggccatc
    25141 tggctctgga aaatctacac tccttaactt attgattgga tttatccagc cgacttcagg
    25201 aaaaattttt ctagatgatc agcctttgga tgaactggat atgcgaagtg tgcgaaatta
    25261 tttagccgtt gttcctcaaa ccactttgtt gttctccgct tcgatcaaag agaacatcac
    25321 ttatggcttg aaaaacgtat caaaagaacg tttagatgaa gtcatcgaag ctgcgcaact
    25381 ttcttcactg attgaccagc ttcctgatgg tctggatacc ttagtaggcg aacatggcaa
    25441 caagctgtct ggtggccaaa agcaacgtat ctccattgca cgagcgttga tacgtcagcc
    25501 aaaaatcatt ttgctagatg aagcaacctc agctttagat aaccagtcag agaaaaaaat
    25561 ccagcaagcc ctgaattacc tgacagaaac acccactact tttattgtgg ctcatcggct
    25621 ttcaacgatc aaagaggcag ataaaatcgt tgtgatcgat gaaggaaaaa tcgttgaaat
    25681 aggtacttac gaaaaactgt tagctcaaaa aggctatttc taccgcttga aaaatgaatg
    25741 acaaaaaat
//
LOCUS       DACDRN010000036        24577 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDRN010000036 DACDRN010000000
VERSION     DACDRN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.036"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(151..549)
                     /locus_tag="IWJ80_001695"
     CDS             complement(151..549)
                     /locus_tag="IWJ80_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP9461411.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(546..1319)
                     /locus_tag="IWJ80_001696"
     CDS             complement(546..1319)
                     /locus_tag="IWJ80_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9461412.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1316..1999)
                     /locus_tag="IWJ80_001697"
     CDS             complement(1316..1999)
                     /locus_tag="IWJ80_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9461413.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2166..3053)
                     /locus_tag="IWJ80_001698"
     CDS             complement(2166..3053)
                     /locus_tag="IWJ80_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9461414.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3050..3919)
                     /locus_tag="IWJ80_001699"
     CDS             complement(3050..3919)
                     /locus_tag="IWJ80_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9461415.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4056..5342)
                     /locus_tag="IWJ80_001700"
     CDS             complement(4056..5342)
                     /locus_tag="IWJ80_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9461416.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5615..6193
                     /locus_tag="IWJ80_001701"
     CDS             5615..6193
                     /locus_tag="IWJ80_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9461417.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6193..7122
                     /locus_tag="IWJ80_001702"
     CDS             6193..7122
                     /locus_tag="IWJ80_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9461418.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7119..8855
                     /locus_tag="IWJ80_001703"
     CDS             7119..8855
                     /locus_tag="IWJ80_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9461419.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8918..9754)
                     /gene="pheA"
                     /locus_tag="IWJ80_001704"
     CDS             complement(8918..9754)
                     /gene="pheA"
                     /locus_tag="IWJ80_001704"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP9461420.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9751..10260)
                     /locus_tag="IWJ80_001705"
     CDS             complement(9751..10260)
                     /locus_tag="IWJ80_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP9461421.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10253..11548)
                     /gene="aroA"
                     /locus_tag="IWJ80_001706"
     CDS             complement(10253..11548)
                     /gene="aroA"
                     /locus_tag="IWJ80_001706"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP9461422.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11561..12652)
                     /locus_tag="IWJ80_001707"
     CDS             complement(11561..12652)
                     /locus_tag="IWJ80_001707"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP9461423.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12669..13835)
                     /gene="aroC"
                     /locus_tag="IWJ80_001708"
     CDS             complement(12669..13835)
                     /gene="aroC"
                     /locus_tag="IWJ80_001708"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP9461424.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13837..14901)
                     /gene="aroB"
                     /locus_tag="IWJ80_001709"
     CDS             complement(13837..14901)
                     /gene="aroB"
                     /locus_tag="IWJ80_001709"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP9461425.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14934..15956)
                     /gene="aroF"
                     /locus_tag="IWJ80_001710"
     CDS             complement(14934..15956)
                     /gene="aroF"
                     /locus_tag="IWJ80_001710"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP9461426.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16615..17589)
                     /locus_tag="IWJ80_001711"
     CDS             complement(16615..17589)
                     /locus_tag="IWJ80_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP9461427.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17570..19453)
                     /locus_tag="IWJ80_001712"
     CDS             complement(17570..19453)
                     /locus_tag="IWJ80_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP9461428.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19471..21696)
                     /locus_tag="IWJ80_001713"
     CDS             complement(19471..21696)
                     /locus_tag="IWJ80_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9461429.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21811..22845
                     /locus_tag="IWJ80_001714"
     CDS             21811..22845
                     /locus_tag="IWJ80_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9461430.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22912..23925)
                     /locus_tag="IWJ80_001715"
     CDS             complement(22912..23925)
                     /locus_tag="IWJ80_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9461431.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24003..24191)
                     /locus_tag="IWJ80_001716"
     CDS             complement(24003..24191)
                     /locus_tag="IWJ80_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP9461432.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7064 a         5340 c         4397 g         7776 t
ORIGIN      
        1 catattttga aaagaaagtc tgatgcttag agtagaattt cataaaaatt tactatttca
       61 taagtattca cgagaaaaat gcaaacaaaa aagcggaaat aacgctcatc cgccttcttg
      121 tttgtgttta tttcctcttc aaacgcttgt ctatgattca gacttttctc tatcgttatt
      181 ttcatctaaa agttcttgca ttccgataat ttctcgatat tgatgaatct tagtgatacc
      241 aatgactttg tctacatcaa tactaaaaca aataccatta cctggaatat cgatgttgat
      301 gccttgacca atcgcatcca ttactttatt cgtcaaacta tcgggtacta atgtcaacac
      361 aatatctttt tctggcgaaa tgtccaaacc taaaaatttc cctgaatcat gtacaccaga
      421 acctcttcca tgtaagatcg ttccgcctgc tgcacctgct tcttttgaaa agtcgataac
      481 atcttcgccc attcctccgt caacgatcgt cacaatcatg tgaagattca atgctacaag
      541 atctttcatt tgctttcccc caatttttgc aatctttctt tttcgattct gtctttttca
      601 tcttttcgtt tataaatata tcctagtact aaagtggata atattggtgt caatgcaatc
      661 attgcaacca caccaaaccc gtcaatgatc ggatctcttc cttcaatttg tgcagcgatt
      721 gccacgctca ttgataaaat aaatgtcgaa cacatcgggc ccgttgctac tccaccgtta
      781 tcaaaagcca ttgctaaaaa gatcttgtcc acttttcgtc ctaaaataaa tgctagcccg
      841 tatcctggga tcaagaaata ataaagagag aatccgacga gcaaacgcca catagacaat
      901 cctactgctg cgccgatccc aattgaaatt accatcagca tgattttcga tcggattctc
      961 ccctcgctca attcttcaac ctgtttgatc attacatgaa tcgccggctc agcaaaccct
     1021 actaagaacc ccattaaaaa gccaagggga accagtatat aattgtaagg caatcccgca
     1081 atagcactgc ctaaatgctg tcctaccggt acataagcca tattgactcc gtgcaggaaa
     1141 aggatcaacc caatagtcgt tatcaagaaa cctttcataa tgtcaaagaa acgctttttg
     1201 cgcatacgaa aactaatgat attcattaaa acaaatagga ttacgatagg tgccactgcc
     1261 aaaatgactt ccatggtaac gatttcgatt ccatctatca cttgctgcca gaaactcatt
     1321 ggaagatcac ccccaaaatc atcacggcaa gaatcggccc taacgaagca ataccgacca
     1381 ttccaaaact gtcattttca tttttctttt gtttgatcat actagtcatc cctccggcaa
     1441 gggccaagat aaatggaaca gttactggac cagtagtcac tccccccgaa tcaaaagcaa
     1501 tcggcataaa ttcggaactg gtgaaaaatg aggcgatcaa aacgcctatg tagcctatag
     1561 ccatcaattg ataataggat agcttgaata cgacccgaag cagcgcaaag gctagaaata
     1621 ctccagtgcc gacgctgacg atcccaatca gcaacactct gccaatctct ccgtcagata
     1681 tatcatatac ttgttgtccc aaaacttgca cggaaggttc agcaatcgtg atcacgatcc
     1741 cgatagcaaa tcccaaactg accaaaacgg tcaaactttt ctttttgatc atatatttcc
     1801 ctactagatc tccgacttcc atcatagaat aatctgctcc aaacaaaaag agcgtcattc
     1861 cgatcatcat gattgccgca ccaaccagaa atgaagcgag gtcttctcct tctaacggtg
     1921 caaaaacaaa cgttaaaatg acaatcagga cagtcatcgg caatatagca gcaatcactt
     1981 ctttaaaatt ttttagcatg tatcctcctc ctttactctt taagtttacc ttaaaatgaa
     2041 agagttttcg agcacaaact taaaacgtta tcatttaatt tttctcaaca taaaataaca
     2101 cacgtcccaa aagtatcttt ttacttggaa aaatgacttt gaaggaacgc gcttattgtg
     2161 tttttttatt ttacagagcc gatcattcct tgaacgaatt gcttttgcaa aacaaaaaat
     2221 acgatggcag ttggtaaagt agcgatgact aatccagcca tgatcattcc atagtctggt
     2281 gtataggaag cacccatagc agataaaaca agcggaaccg tccgtttatc aggagattgc
     2341 aaagctacta agggccataa ataattgttc cagctactca tgaatgtgat gattgcagcc
     2401 gcggcatagg tatttttagc cgtcggcata tacactctaa aaaaaatact tagttctttt
     2461 agaccgtcta atcgagcggc ttctactaga tcttttggaa aagatttaac attttgtcga
     2521 aagaagaaga tcaaaaaagc agtgctaaca gatggcagga tgacaaccaa gaaactgttg
     2581 attcccaaaa ccgttccgtt caattgtgaa aacattcgat ataaagggat catcaatgcc
     2641 gcaaaaggaa ccatcatcga tagcagtaga atattgaaaa ttaggtcttt tcgtttactt
     2701 tgataaatct caaatccata tcctgccatt gacgaaatca gcaatgcaaa cacagtggtc
     2761 accaaagcga tcatcgctga attccaaagg gaacgagtaa aattcagctc attggaaaac
     2821 aaattttgaa aattgatgat caagttactg ccaatcttta tttttcccga tgtgatatcc
     2881 acgggtgtat tcgtcattcc caaaaccatc cagacaaaag ggaaaatgga aataaatgat
     2941 aggatgctta atgctgtata ctttaatact gacccaattg tcgaacgaag ttctaatgct
     3001 tgttttttga cgttggggcg ttcgattgtt tctttttcta ccatattttt catcgacttt
     3061 ctcctcctac tttcgtttgg attatcgaca atatcacaat aaaggaaaca ataacaaagg
     3121 aaactgctgc cgcatatcca aaattaggcg tgaatttata actcaaatta tagatgtatt
     3181 gagatagggt ggtcgttgca tttgctggtc ccccgccagt aatattttgt acttcatcaa
     3241 aaagctgcaa tgtcccgatc gtcgaagtaa tggaagtaaa caaaatgatt ggttttaaat
     3301 ttgggatcgt cactttgata aactgctgcc atttagaagc accgtcaata gatgctgctt
     3361 cataaatctc cgaatcaata ttttgcatgc ctgaaaggaa aaaaatcata ttgtatccag
     3421 tccaacgcca agtgatcgaa agaatgatca gaactctcgc ccaaattgga tgagtcaacc
     3481 agccaatcgg tgtgtcaatc ataccaatgc ccaacaacaa gttgtttaca aggccagatt
     3541 gagaaaatag acttttcatg atcagagaat aactgaccat cgaagtgata catggaagaa
     3601 agatagcggt tcgaaacaac cctttatatt tcaacttttt atcatttaaa aggttggaaa
     3661 taaaaagtgc tagaaacaac atgatcggta cttgtacaag taaatacagg aatgtattaa
     3721 aaaaagcttt tttaaaagtt gcatctgtca acatccgctg ataattacct aacccgttaa
     3781 atgtcatatt gacaggggga ccactttgaa aagacgtgat caaagccaaa accatcggaa
     3841 taaaaaccat gattgatatc aaaatgactg gcaagattaa aaaagcatgc ccgtttttga
     3901 ataaatcttt ctgcttcatt cttttccctc caattctatc attgattatc tacatgaaaa
     3961 gacaagaaag acaatatctg taaggcagac gtgatctttt ttgtcttttc gtttgtctga
     4021 tgaaaaagcg aagaaattca cttttttacc accgatcaat tagccaattg tgcttctact
     4081 tgtttttgtg tatctgctag taccttgtct gtctcttctc cattgatgat tcgatgaaga
     4141 gcttcagcta cgactgcttc aattgcatag gtctcatgtc cgtaattcac ttctggtatt
     4201 tcagatgtcc atgttgaaaa atcgtcaaag atcttttgac cactgtaaaa ttcagaagct
     4261 tcttgataag cttcttgttc attcgcgcta agcatcgtag atactagtcc gatttctttc
     4321 gcaagagtgc ccatcagttc ttcattcgaa gcaaacgttg cttctaggaa atctttcgct
     4381 gaatcttctc cagctactcc tttgatcaca taccagccag cgccgccaag gttagaagct
     4441 tgcgcttttt gcagattttc tggaagtgca ggtattggtg caatcttcca ttttccagat
     4501 tggttttctg ctccttggat cgttgaagaa taccaagcgc cagttgggct agaagctact
     4561 gctcctgatt gtatcgcagt tactccagca ttccagtcag aggtttgttc gactagatct
     4621 tctttcaaaa gagtggcaaa caattcaaga ccatacttta aagatgcatt gtcttggatc
     4681 gtgactgtct ccccatcttc agatgtatac cattctcctg cctgttgcat aatcatcctt
     4741 actcgaccaa ggtcagaagg attgacttca gcgattttct ttcccgtctt ttctttgaca
     4801 tctcgcgcta cttgaatata ctcgtcccac ttcagattct ccatatcctc ttctgtataa
     4861 cctgcttctg ccatcagatc cgtacgatag aagtttgctg ttacgcccga atcgaaagga
     4921 acaccgtata ttttatctcc tactttgttt accgcaaatt tatacggtgc aaaatgatct
     4981 tcctttacaa tagcagacaa attagcgaat gcatctggat aagaagtcaa atagccttgt
     5041 atcctataat cttcaatcaa gacgatattg ggcaatcctt ctgtattccc gctcgctaaa
     5101 gcggtgttca gtttctgaac gatatcatcc tgagacatcg taacaacttc tatctctgtt
     5161 tcttcgttct cataaacttt ttttgcttca ttcactgctt tgatattgaa tgtctcatcc
     5221 cacgcccaga cagtgacttt ttcttctttg tctgctgtac tactagcact tccgcatcct
     5281 gttaagaaac ctgatgctgc cgctaaacaa acgactccag caattaactt tctgatgttc
     5341 atttttatcc gctccttttc catccattag ttgtttacgt ttgctacatt catcatacca
     5401 aaatttgaaa acggtttctt tattattcag atacttcaaa aatttctcct aaaataataa
     5461 aaaaatacaa atcaactatg acaaaaggaa taatcttagc atctagacca aagatcgaaa
     5521 tggtgaggtt tctttaaaat aaaaatatcc ttgcattttc tcctagaaat aagtattttt
     5581 aaattacaag actattctta aggcgggtaa aaaaatgcca atacgcgtaa ttcttatcga
     5641 tgatgaacag acgattttag atggaatgaa acagttgatc aattggtctg agcacgggtt
     5701 tcaattaata gggacttttt ccagtccgat gaaagcgctt gatttttctt tggtacatgc
     5761 aatcgatatc gtggtcacag atgtaatgat gcctgaatta aatggaatcg aattatcgcg
     5821 gctattaaaa cagcaacaac ctgaaacaca gatccttatc ctctctagct atgatgaatt
     5881 tgatatggtt aaagattctt ttaaagaagg cgtcgctgat tatcttttaa agcctcgatt
     5941 gagtcctgat ttttttctaa attccttaac tgccgtttca caaaaaatca aaaaaaagcc
     6001 caacaaaagc ccaatcacca atcgttatga gcagatctgc gagaatcttt cacagcagat
     6061 tgctggacaa actcgtccat tgcttgaccc tcttcttttt gaacacacct tgtttttctg
     6121 ttatataccg aaaaacggcc attcagttca aaacaaggct ctaaacaaaa acagtcgatc
     6181 acccattcat tgatggatag cgacgattcg tttactattt atcctgtttc taccttttcg
     6241 gaagaatccg gctatgtaat caatactgat tccaaagata cacttaggaa gtttgttcaa
     6301 aagtttcgtc aagaagtttt tccaggtgat ttttttgttg tttctgattc ttttgcaatc
     6361 caagatatca atgaaatatt ccaagagatc agaaattgca ccaaaggaca acgtttctat
     6421 cataagggaa agaaccttgc ttatcagcat gaacttcttc cgttattaac caaccacgag
     6481 cagcaagcaa gtagttattt aacaaaaatc gttcataaag actacgcttt agctgtcaaa
     6541 gaacttgaga atttcttcac gacgatattg gaacagccta ttgatccagc ttatttgaaa
     6601 catgaggcaa tcaatcgttt ctatgcatta ctcaacgctt tggctgacga gcatgttggg
     6661 aatgaagaac tttctttgct caagatcgcc cttccttctt tattagccga tgctgagtat
     6721 ttcgaagatt tttctattct tgtcaaagaa acacttgaga aactcaccca cttttctgca
     6781 aatgcaaaaa aagacaattt cgatatcttg actttgatct acgagttcgt ccaggaaaat
     6841 tatgagaaag aaatcaattt acaactttta tccgaaaaat atcatttttc atatagctat
     6901 ctctcagcca tctttactga aaaatttggt atcaactttt caaaatatct aaaaaaagtc
     6961 cgtatcagta aagccaagct ctttttgacg gaaacacggc ttactttgac ggaaatctgt
     7021 gagaaagtcg gctataccga actgggttat ttctccagag tttttaaaga agagacagga
     7081 atgacacctt ctcaataccg taaaggaact tttgtaaaat gagaaaaaaa atcaaagagc
     7141 atgaactctt tgccaaactt ctgttcattt tgttcatcgg tctttttctg caagccatcg
     7201 tcatttcttt gtttatttat catcgatcaa gggatgccta tatccagctg ttcaatcaat
     7261 cgaatgatgt ggtactaaaa aagattcaat ctgaatttga aagtttgaat gatacaatcg
     7321 agaatacatt agccgctttt gattctaatc cagctgtcaa aagctatttt tccaatgatc
     7381 cagcccaaca tatggaacag cttcagcaac taagaacaat ccaaaaaatg aatgactctc
     7441 tttcaaaaat ccatccaatg atcgattacg atgtattgat ctttggagaa aatggaagaa
     7501 cttttgtcgg aaacgacatg ctcactgccg tatctgccga ttcttttttt caatctgcga
     7561 ttgctcagag agtgaatgaa cgtgctgctg atacacaaat gctttttgca caccacggat
     7621 tgactcttcg cgataagaaa gcacccagcg tcttctttgt caaaaaactg aaaaatacat
     7681 tgaatcacgt ttacggatat gctgttttat cgatctcatc caagcaacta gcgaatcttt
     7741 ttcaatctgt agtaaatcct gagatatcga agatctcctt catgaacgaa gaacagacga
     7801 tcatcgcatc aaatgaagaa aatacaattg ggagccgatc aatgattttc aaaaaactaa
     7861 cagtaggtga aacaacgatt tcaaatggac agcagttgac ccgtcttcct ctgtaccgcc
     7921 aggattgtac attactcagt caagtcgatc ttcattcttt atctaaccaa atgggcgtga
     7981 tttttccgat tattttgtat aatatcgtga cacttgcttt tgctggctca ttggtattta
     8041 tgtatttgga tcgacacaca aaatcgatct accgcttgat tgattcgcta aaacggctag
     8101 aggaagatgg cttaaaagct actgttcctg tacaaggaag ccatgaagtc caattgctag
     8161 ggaatacgat caatcaattg ctaagtaatg ctcagcatca ttatgaacag acgatccaag
     8221 acgaaaagaa gaaacgttcg cttgaaatcc aaactatgca agcacagata cagccccatt
     8281 ttatttataa tacgctgact agcttgaaat ttttgatttg gcagaaaaaa acagatgaag
     8341 cagttcaagg aatcgacagt ttcattactt tactaagaag tacgattggg aaaaaagaag
     8401 aagtgattcc agtcaaggaa gaaatcaaaa gcgtacaaag ttacatcgat cttttgagta
     8461 tgcgatatgg cgaaagaatc tcggctaaaa taatgattcc tgatgaactg cagtcattat
     8521 ctatgccgaa tatgatcctt caaccaatta tcgaaaacgc ctatcttcat gcttttcaca
     8581 ataaaacgca aggattcatc acggtgtacg gcaaaacaaa aaatcagctt cttttattcg
     8641 aagtgattga taacggcgat ggcttcgata ctgaaaatac caaacaaaaa aatgatcatt
     8701 tttcaggaat cggcatcagc catgtagatg agcggatacg cctaatgtat ggcgatgcat
     8761 atggcttaac gatccaatca gtgattggga caggaacgac tgtaacgatt tgtctaccgc
     8821 ttcttcctaa aaatcaatca gaatatcaaa aataaagaat agccaagcgc tgattttttt
     8881 attttaagtt aaataaatca atcagtattc ttgactttca tctacgcgtc tcttctactg
     8941 tcagcactgg ataacagccc aatatttgac tttctcctcc gagcatcttg atttcttgga
     9001 gcgcattctc tactaatgtc attggacggt ccaacaacaa atcgatcacg aaaaaatatt
     9061 ctcccaagct agttttcaat ggtcgggatt caatcttact taagttgatt tcccgccagc
     9121 caaaagctgc cagcatttta tgaagcatcc ctggccgatt agcaggtagt gtcaagatga
     9181 cactcatttt tactggagct gactgctggc tggtcatctt tcggtcaccg acgatccaaa
     9241 aacgtgtctg gttcaactca ttatcttgga tattctcagc taagatctcc aatccaacat
     9301 gctgtgctgt ttctaatgaa gcaatcgctg ctgcgtcttc tttcggatgt tctgctacat
     9361 acattgctgc agcagttgtt gattctgttg ctactaaagg aacattagga taatgcgttt
     9421 ctaaaaactg ttgtgattgt gctagtgctt gtggatgtga aaggattttt gtaatctttg
     9481 ttgctggatt gcctaaaagc tgctgcttga taggaagaac gatttcactt ttaacttcga
     9541 cttctggatg tttggaaaga agatcgattg tgtgatgaac agctccttcc aatgaattct
     9601 ctacaggaac gacagccaga tcgacttgtt tgcgaaacaa agcgcgcaaa caaacaggga
     9661 tggaagcata cgcagtcaat tgttctgtag gaaatagctg acaagccgcc tgataagtga
     9721 atgaactttc tggccctaga tagctgacgt tcatatgctt ttgatccttt ctataatggt
     9781 atcgatgatc ttttctggtg tttgattggt cgtgttgatt cgatgtgtcg cactttcttc
     9841 ataccaagaa agtcgttgtg ccaataatgc ttttatttct tctcttgttt ttccaagagc
     9901 taaaggtcga atagacgttt catcttgttg gatacgatca actagacagt ccgcttctgc
     9961 ctctaagtaa agaacaatcg gctgtgtttt taaaaaacgc cgattttctg cactttggac
    10021 aatccctcca ccagtcgcga taattttgtc ttcatttgac agctttctta aaatatctga
    10081 ctccactttt cgaaagcttt tttcaccgta atagtgaaaa tattcggcaa tcgacaattg
    10141 gatatgttcc tcaatctttg tatctaaatc tacatactct ttttgtaaac attgagctaa
    10201 ccctttaccg attgttgttt ttccagcgcc catgaaacca atcagcaaaa tgctagccat
    10261 gagcttctcc tcctgacaca agttctgcta aatcgtcaaa aaatgccgga taagagattg
    10321 aaacagcttc agctccttct agttcgcaag gtccatcagc tatcaaagca gctacttgca
    10381 gcatcatacc gattcgatga tcgcctctgc tggacacttt tgcgccatgc agaggtgtcg
    10441 gcccatggat gatcagaccg tctgctgtcg cttttatctt tgctcccatt ttctgtaatt
    10501 cttctgccac cgcatcaatt ctgttggttt cttttacttt taattcttct gcgtctttga
    10561 tgatcgtgat tccttctgct tgagtagcca ctaaagctag aatgggcaat tcatcaatca
    10621 gacgcggaat gatttctcca ccaatcgttg ctttcttcaa atgactcgtc ttgacactta
    10681 aatcagccga ttggttctgt tcattgtgat tcgtctgggt aatcgctgcg cccatctcct
    10741 ccaacacatc taagatacca gttcttgttg gattgacccc gacatttttt aacaacagtt
    10801 ggctttccgg aacaagaagt cctgctgcta gaaagaaagc agctgatgaa atatcacctg
    10861 gaacagttat ctgttgacca gttaattttt gcggtccggt cactatgatc gttttatctt
    10921 ccactgtgat ccttccgcca aattgacgaa tcatttcttc cgtatggtta cgggaacgtt
    10981 ctttttcaac gattacggat gtgccttctg cttgtaatgc cgcaaacagc aaagcagatt
    11041 tgacttgcgc actggctaca ggcatattgt atcggatagg tgataaagag cgggttcctg
    11101 tcacagtgat tggtagatac tggtcatttt cttctccttg caagtcagcg cccatctcgc
    11161 gtaaaggttc catcactcgc cccattggac gtttagacaa cgaagcatcc ccgaacagag
    11221 tagtcgtaaa aggctgtcca gccaaaatac ccatcaataa acgtatggtc gtaccggagt
    11281 ttcccatgtc aattgattcg gttggttgga caaatgagtg gctaccgcga ccatgaatct
    11341 tgatcacctc ttcctcttct tcgatctcga cacctaattg ttggaaggca tgtaaagtac
    11401 tcagacaatc ttctgcgcgc aaaaaatttt gtatagtcgt cgttccttca gcaattgctc
    11461 caaacatgat gctgcgatgg gaaattgatt tatctgctgg tatcctaaca gtccctctta
    11521 atccatgtat ttgttgtaat aattgcattt atccagcctc ctatttgatc cagcaatgat
    11581 aactggtatt agacataatg attctttttg cttgtaacaa atcctgttgg tttttaaaac
    11641 tgatttgcag tattcctaaa atatcttccc tggtctcttg gatcttcaaa ttaatcaaag
    11701 aaactcccgc ttttccgatc agtccagtta cttctgcaat aacgccaggt tcatctggca
    11761 catcgataaa taaatcaaaa aaggcaggaa tcgcgccttg cttatggaca ggcaactggt
    11821 ctcgactttc ttttgcccga ttgaaaaaag aaaagatttc cgattggttc tcagtcatga
    11881 tccattctgc tatttgtgtc atttcttgtt gccaatctgc caatcgttcc aataatatct
    11941 gcttattgct caacaaaata tctgtccaca tgacaggatc tgaggaagcg atcctagtga
    12001 tatctcgaaa gcctcctgct gccagttgtt tggctcgcgg atactcttga ttgaattgtt
    12061 ctgcctgatt gactaagcca gctgccagaa tatgtggaaa atgacttagc attcctgtta
    12121 tacggtcatg ttctcttgct gtaagagtta catattttgc tttggttccg caaaatagtt
    12181 cttgcagttt ttcaacatct tttttcgtct tttccaaagg tgtaaagatg taataggcat
    12241 tttcaaatag tttagcatct gctgcctgta tacctgattt atgagaacca gccattggat
    12301 gtccaccgac aaaccgaaga tttttctgct cagcggatgt cacgacttgc tgcttcgtac
    12361 tgctgacatc cgtaatgatg acgtgatcag acaagttgat tctttcaagg acatcaagat
    12421 aagctaaggt tgtacggacg ggaacggcta ataagataac gtctgcttgt tctgcacctg
    12481 tttcaaaatc acttgcaacg taatcaacga tctgctgttc tactgcttgc tcacgggttt
    12541 ttgactgact atcccaacca actactatca gttctggatt ggtctgacga atacaaagtg
    12601 ccatagaagc accaatcagt ccaagcccca cgataaaaac atgttgtgcc atcttatttg
    12661 cccccttttt agtaatgttt caagtagtca cggtactcct tcactgcagc tttcaactca
    12721 acaagcgaat cacttgggaa ttttgacaat agctctgtcg ctaccacagt ggctacaaca
    12781 ttttccgcaa cgacgctagc tgcaggaact gctgtactat ccgaacgttc tacacttgct
    12841 ttgtaaggtt cttttgtatc gatatcaaca ctttgaagtg gcttgtacaa tgtaggaatg
    12901 ggtttcatga ccccgcgaat gacaagtggt tcgccattgg tcattccgcc ttcgaagccg
    12961 cctagattat tggtacgtcg gaaatagcct ctttcttctt cccataggat ctcatccatc
    13021 acttctgagc ctggtaatga accagaagtg aatccgaccc caaattctac gcctttaaag
    13081 gcattgatac taaccacagc ttgtgcgatt ttggcatcta gcttcgtgtc ccattgtacg
    13141 tagcttccta atccagctgg aacgcctcca acgagcactt ctacaattcc tccgatcgta
    13201 tctccatttt tctttgtctg atcgatcaaa tcttttattc tttgctcaac tgctggatca
    13261 acaactcgta cttctgacgc ttcagaaatt tctttgattt cttgaatcga gatatttcca
    13321 ggtatttctg cttggattcc accaagacaa atcacgtgtc ctgctacgat gatgtcaagt
    13381 tcttccagaa ttttcttggc aattgcgccg attgctacac gcatggtcgt ctctcttgca
    13441 gaggaccgtt caagcacatt ccgaagatct cgatgatggt atttcatacc gcctaccaaa
    13501 tccgcgtgtc ctggacgcgg acgtgcaact cgccgcagtg attggtcttt ttcaggcact
    13561 tctgctacag acatgacagt tgtccaattt ttccaatctt tattttctac cactaaagtg
    13621 acaggtgaac caattgtttt gccgtgacgg atgccagacg tgatccgaac acgatctttt
    13681 tcgatcttca tccttcctcc tctgccgtaa ccagcttgtc tgcgagcgag ttcgtgattg
    13741 atttcttctt ctgacagagg taatcctgct ggtagtcctt caataatggc tgttaattct
    13801 ggtccatggg attctcccgc agtcatataa cgcatcttac agtcctccta agtaatcttt
    13861 gaatgtctct gtgggaacag gaacgatttt tgcttgccca atcttttcta gcaagatgat
    13921 attcaattgg cttcctcttg ctttcttatc atgggttatc gcgtggaata attcatctgg
    13981 atgacttaga tcgtacgtaa tcggcagatg gaatttttga agtatccttt ctaaagcagc
    14041 tgtcgatcca gttggagaca agccgatttt ttctgcatga gaagtgatgg ctaccattcc
    14101 aatagctact ccttctccat gactgactac cccatatcct gctgtatttt caattgcatg
    14161 gccaattgta tgaccaaaat ttaaaataag tcgatttcct tggtcaaaga catcttcttc
    14221 cactactttt cgtttgattt ccaacgtagc agtaatgatt ggaacagcat gttccagaag
    14281 atctttttcg tctttcagct cgttcagctg tttccaaagc gttgcatctg ctatagctgc
    14341 tgatttgatg atttcagcga ttccttctcg taaacgacgt accggcaaag tttttagaac
    14401 ctcaggatca atcaatacac cagctggctg gctaaatgtt ccgacaaggt ttttcgcttt
    14461 gtttgtattg actgctgtct tcccgccaat actagaatct acttgtgcaa gtagcgtagt
    14521 cggaatttga acgaaatgga tcccacgcat gaacgtcgag gccacgaatg ctgctagatc
    14581 acccaccatt cctccaccaa gtgcaagtat cccatcgctg cgggtaaaat cattatctgc
    14641 taaaaaatcg tataactcag ctgcttgttc taaagattta ctggtctcac ccgccggtac
    14701 gattgaaagg actggttcaa aattagcttg tctcaattgg aacatgattt tttctgcata
    14761 tagtggagca acgtttgtat cagtgatcac agctactttt tgaggtctcc aaatcgtttg
    14821 aagccatgat ccagcctgtt ctagtgcgcc tttttcaata tgaatatcgt atttttggtt
    14881 atcaacatgt acagtaagca ttcgtttcac ttctttcttt taaaaagctg gaattaatcg
    14941 agtgtctgaa tcttttccat tgcttctttc atgatcgcaa cttctttcat catgcgtgag
    15001 taagtctttt cattcaacga ttggggaccg tctgaccaag catttgcagg atcaggatgg
    15061 atctctacga tcatgccatc tgctccgctt gccacacccg cacgagccat tggtggtaca
    15121 agttcccaaa ttcctgtacc atgacttgga tcaacaatca cagggaaatg agttaatttt
    15181 ttcatcaatg ggacagcact taaatcaaac gtattacgtg tcgctgtttc gtaagtccga
    15241 atcccacgtt cgataaaaat gacaggagat ttgtcttcga ctgcaatgta ttctgcagca
    15301 ttaagccatt cattgatcgt accggaaatt ccacgtttca atccaacagg tttacctgtt
    15361 ttaccgacag cagatagcag tttaaaattt tgcatattac gtgcgccaat ctgtaggata
    15421 tcgctgtact cagccaccat atcgatatgc ccttcatcca tcacttcagt aatcactttc
    15481 attccaaatt catcagccgc ttgacggatg tatttcaatc cttcttcttc taaaccttgg
    15541 aaagcatatg gagaagtacg tggtttgaag gcaccacctc gcagaatttt tgcgcccccg
    15601 gctttggcca ttcgcgcaca ttctctgatt tggtctaatc cttcgataga acaaggacca
    15661 gccatcgtca cgaaactgcc gtcaccaatt tttactccgt ctacatccac gactgtattg
    15721 ccaggatgga attctcgaga agtcaatttg tatgtgttta aaatacgcac agcattttct
    15781 actccttcat agcgtgtaaa tgctccttct tgtaaacttc gcgtatcacc ttttagtcca
    15841 atcactactc gttctgttcc ttgatcgatt gagactttga atccttcttt ttcgatatga
    15901 tcgacgactt tttttatttg ttcttccgtt gcttcttttt tcataattac tatcattgtt
    15961 tttcccgctt tcttttgttt acttatctat cttggctgat tttctctact ttttcaaggt
    16021 ggttttacat taattcctac taaatgaccg tgtcctgttg ttctcatttt cgctccttct
    16081 ttcctactta tgacccagat aatcaacaaa aaaagatgtc cctgtaggaa attgcctcta
    16141 cagtgaacat cttcaacgag ccttaaaaag cgctgcaaaa atataaaata gccactatag
    16201 atgaatttct ctacgtggct gtaagtcgtg tttaaaaatc tttagccatc atagatacaa
    16261 acctggtttg tttgtaccca cgaagtcatg aatacaaacc tatctaaaat agctaaagta
    16321 atacgtattc agttttgtaa gttttctaga tttcataaaa aatcgctcca aactgatgta
    16381 agaattgtac ctagtataga cataaaaaaa tcgactgtca atcattttta tgaaactttt
    16441 tttaattttc tgatagttaa ctttgttttt cagaaaaata acaattggtt ttattgaaga
    16501 aaatagtcat gaacaaaagt atctgagaac ttttttactc tcttgttcat gacatataga
    16561 ataaagcagc tttgtcttgt aaactccgtt tttcaaataa taatctaaag attgctactg
    16621 aataataaag ccgatttcat ggtcctcttc gctgctgatt cggtaaggtg actcaacatc
    16681 cgctccccag ctattgatac ccccgactcc tcttactttt gcaaaaatcg taagaaccgt
    16741 tcgtctttct ggcggtagtt cttctatatg tgttgcctgt tccagttctt ctgctttgaa
    16801 tggtaaagcg gaaaaagcaa atggcgtatc ctgctgttca aatcgcaacg aaaaatcttc
    16861 ttgatcttta tccgtattat ccaacgtttg atttcttgtt agttctaccc atttcgtttc
    16921 catatgcatg ccgcattctt ggggaacaag gtatggggaa acggggagtc ctgccacttt
    16981 ataaatacct tcgacaccac cagccatccg atccggatat gtttcccctg ataggccttc
    17041 atactcaaaa aattttgctt tcgttggcat aatgaacctt aaacccaatg cgggtaattc
    17101 cggtaatcct ttttgtccat aataatgcat attgacaagc attttaccct ctccagcgat
    17161 ttggtaggaa attgtcactt gggtttttgg tactgtgttg atttccagtg taaaaatgat
    17221 ttcaaccgtt tcagcgtatt cattacttga atattgatta tttactggag caatcggcaa
    17281 atcgatcttt ttattgtcca ccagtacttc tatttctttg acttttggat acaatccggc
    17341 tcctaaccac ataccggatt taaatccaaa tccgctacct ctgtcattat cggtcgttgc
    17401 tcgccaaaaa actggaagtg tttcccggta tagccattct tttccgtttt tattgagcga
    17461 ttccagtccg cctcttgtat aagaaaaaat ataatgcgta ttccccgatt tgacacctaa
    17521 tgtacagtcg ccgaagacga ttgcaacttt ttccgtatcc atatgacttt tatttactgt
    17581 atttttcata ataatatctt acctcctgca tggcgggttt ttcttgtcca tctgcaaaaa
    17641 cgatcccatt ccctgagaat tcatagtcac ttggtttatc gtcaaagtca ccgccatagc
    17701 gcatcacttc tctgcctgtg attggatcag tcacttggat cgcctgatct ttaaaatccc
    17761 atatatagcc tccttggtac atttcataac gatcaagcaa cgtcatgtaa gaatccattc
    17821 cacctaacga attccccatg tcatgcatat attcacataa gatgaacggt ttttccgctt
    17881 tattttcaag gtagtcaatg atctcttctg gtggcgcata catgcagctt tttacatctg
    17941 aaatcgaatc ttcgaatgct ggatttggaa aattcccttc ataatggaca agtcgatcgg
    18001 ggtctacttt tttgaagtaa tcgttcattt gtcgaatgtt ttcttccgca taagattcat
    18061 ttccaagaga ccaaaaaaga atacttggat ggttttttaa tgtttcgaaa ttcgtttttg
    18121 ctcgatcaag aacagccgcc tgccactgag gtactgcacc tggtacgttg taagaaggct
    18181 caaatgcgcc cattttttgc catgagccat gggattcgag gttcgtttcc gccatcacat
    18241 ggattcctgc ttggtcgcat aaataatacc aagtgatctg gttggggtaa tgagatgtcc
    18301 gtacgccgtt gatctttgca ttttttatga tagccaaatc tttttccata tcttctatag
    18361 tgatggcgcg tccttttttc ggatgccact catgtcgatt taccccgttg ataatcatcc
    18421 gttttccatt gaagtagatg attttatctt tgatttcgat tttacgaaaa ccgaaggaat
    18481 agtcaatata ctccgttact cttttttctt cgtctatcgt ctccaattca agcttatata
    18541 aattgggctg aaaatgcccc cacagtgcca cattttcaaa tccatcgatg cgaatcgtca
    18601 tttgctcttt taacggatat tgctgttgat agatttcctt cccatcagga tctagtatcg
    18661 ctacattcag ctgactgttt ttggctactt gctcaaaaga caaatctact tcaagtttgc
    18721 cattttgaca tttgtcatct aagcgaggtt tgatccaaag atcttctaaa tggttttgtc
    18781 ctcgagcaac taagaagaca tctctgaaga tcccaaaaaa tcggaaaaag tcctgatctt
    18841 ccaagaatgc agctgtactt cttttgtaaa cctccacagc tagaatattt tcttcttcca
    18901 aataaggtgt taaatcaaat tcagacggag tgaacgaatc ttcggcatac ccgataaaat
    18961 gaccattcaa ccaaataaac attgcttgtt ctacaccagc aaaatgaatc gagatttgtt
    19021 ttccctttag accttcttct aatcgaaaag tcgtcaaata cgaaccaacc ggattgtctt
    19081 tagcagctga aaagatccct tccgcctctt tctcagggct tgtcgtgaaa gggggacggc
    19141 gaaatacatg tccctcccac ggatacatcg tattgatgta atggatcttg tcataatttg
    19201 ctaattcaat atgactagga acagtgattt catcaaaaga acaggcatca aaatcttttt
    19261 tataaaatgt ttttggtcgt accgaaggat ttggcgaata acaaaaacgc catgatccat
    19321 ttaaagactg tttaagggag gatgtggttt ctacttcttc ccttgttgca taacaaacat
    19381 gatcgctatg tgcatttgat tggttcactc taaaaacttg tggatcatct aaccatttta
    19441 tatttgcttt catacttttt tccttctctc tcataacttt ttgaaatgaa gcagttctgt
    19501 ttggaaatca ccttgaaaat cggggaagta aagtccaact gtcatcaatt cggcccccga
    19561 aaatatccgc ttcgaggtct gttcctgata aagtgcctca tcatctaacc ccatcagata
    19621 tacttcatgg aacgctggtt gagctgaagc tagtatcctc cctaaaaaca caatagcttc
    19681 atcttgttta ggagacacaa acatccatgc ggcaatattt tcttcaaaag gactcgccaa
    19741 acgataataa tggccatact ggatcaccgg acgaatcgtt tgataggata taatttggtc
    19801 ttttacttgc tttttttctt caggagacag ctttgtcaaa tccagttcat aaccaaaaac
    19861 agcgctcata gctgtttctc ctcttgtttt caacgaagta gatctgtcgg tctgatgatt
    19921 tggtacagct gatacatgag ctgtgatgga cgaaatcgga taagcaaggc ttgttccata
    19981 ttggattttc atacgctcca ctgcatccgt gttatcactt gtccatgact gcggcatata
    20041 ataaataaac ccggcatcaa accgtccacc tccaccagaa cagccttccc ataagatttc
    20101 cggataatca gttgtcaaat gttcaagtag ttcataaagc ccgagcatat aacgatgcgc
    20161 tacttctcct tgtctctgtg ggtcaagggt aatcgaatag acatctgaaa gcgaacggtt
    20221 catatcccat ttgatgtagt caagaggaat cgtatctaaa atctttctca tttgtttctc
    20281 aatcgtctgc cgaacatcta accttgtaaa atccagtaca tgttgtgatc tggaagcaga
    20341 aggcattcta cctggctcct gcataagaaa atcaggatgt gttcgataca gttcagaatc
    20401 aaccgagatc atttcaggtt caaaccataa accaaatttc aatcctttgc tatgagcata
    20461 atctgctatt tcacgtaatc catttgtcaa cttgccttca tactcgaacc agtctcctaa
    20521 tgaagaattg tccgcatctc tttttccaaa ccagccatca tctaatacga acatttcgat
    20581 gcctagctcc tttgcttcat caatgatcgc cttgattttt tcactattga agtcaaagta
    20641 agtggcttcc cagttattga ccagaatcgg ccgttcagca aattgatgac gacctcttgc
    20701 gattctttca cgtagcaact catggtggat ctgactcatt ttgttcaatc cctgacttga
    20761 gtaagaaagg atcatttcag gtgtttgaaa agactggcct gcattcaatt cccacgaaaa
    20821 acgatggctg ttgatcccac cgacgactct caattgattg atctgatctt tttcaagttc
    20881 gaaagaatgg ttgccggaat aaacaaattg aagtccgatc gcttctccag tattttctgt
    20941 cgtatgatga tgaactaaag caataaagtt attcatatgg tgactgctcg ttcctcgacg
    21001 gctttcaaat acttttgttc cagataaaac agattgccgt gaaatctgtc tttcattgac
    21061 atgtgctcct ggcaaagcaa tgacttcatc aaatcgccta gtatgggcaa agtctagctg
    21121 gaaactagcc gctttttcta gtttgattgt ttctccggtt tcatttctta tctttacaga
    21181 tcgtgtgatc actgctctat cagtaaatat ggtatagaac aaatcgaaat aaatcgctgt
    21241 ttctctatcc ttaagtgaaa tcaccaacgt ttgtgcctct gacttgtcca tcacataagt
    21301 ttgaggcaag ccttcgatct ctggcttccc atctgtaata taatggctgt catatctcag
    21361 atccagtaag tctgatccat tcttgcgctg aatgatcgat gcgggcgttc gaaagtccat
    21421 gctgccatga gaagagtatt cttgcggtag tgtatctttc gacagagacc ggtcaggatt
    21481 taagggaaca tttcctgaaa atccacgatc tcttcgggga taatttttat tgtctttgta
    21541 ctgcttgact ggtttgccaa aataaatatg cgccaataca ttgctttctt ctatctttat
    21601 cagataagaa agaaatgtat ttcgtaaatg gaagactcgg ctcttttcat caaataagat
    21661 gaaattttta tctgtttttc tatgcgtaat atccatcatt tcactcccaa caaaacggaa
    21721 accgtttact tttttttata attcctgtat actgtttact aaacaatttt gtcaataaaa
    21781 attttttatt tttttgagga gtgtgtaaaa atggttggaa ttagagatgt cgcacgaaag
    21841 gcaaatgtct ctcctgctac agtttcccgt gtgttgaata acgatcagac gatccatgtg
    21901 gctcagacta ctcggcaacg gatagtagag gcagctgctg cgttggatta tgacatcagt
    21961 actcgtaaat atatcaaaaa gagaatgcct tcgattggtg tgatctctac gatcggcaaa
    22021 gcgagcgaag aacaggatat ctattacaaa catttgcgag tagggatgga agaagaagct
    22081 cgacgtctgc atttagggat gaatcgaatc tataatctat ctgataatcc taaacaatgg
    22141 gaagatctag atcagttagg tgcggtgatc gttgttggga ctgttacgaa agaatcgatt
    22201 accgccttgt taaaacaaaa catgcaaata gtcgtcgtgg ataaccctga catccagcaa
    22261 gaggtcgata tggtgtatgc agatcttgaa cggatgacta aaaccgtact ggaattgttc
    22321 ttagaaaatg gtcataaaag aatagcctat atcgggggat ataaagtcga tatggatgaa
    22381 ttcggtggga agcagatttc tataaatgaa aagaggatgc gtgcctatca gcattttatg
    22441 atcgatcatc acttagaaaa atatatgtct tatcatctag gtggctggac agaaaaacaa
    22501 ggtgaggtac ttgcgactaa tctattagaa aatgaatccc ctcttcctac tgccattctt
    22561 gtcgggagcg atccaatggc aattggtgtc tatcgggcat tgaaacaaaa aaatatccaa
    22621 attggagaag agattgttgt agcaagcttt gataatatcg agacaatcgc aaacctctcc
    22681 cctggcttga ccagtgttca aatcaatgcg aaatcaattg ggcaagcagc tgttcgaatg
    22741 gcattggagc gaattgacgg tatacgggac gaaccaatca ttttgactta tcctgcacga
    22801 ttgattgtta gggaaagttt tatcccaaga acaaaaaggt attgaaagag aggttgtgaa
    22861 aagccgcttc gtttcttcat actcaaaacg aaacggcttt ttcacaacct cttacttata
    22921 cttattcaca aaatccaatg tctgctggta acaattttgc gattctttta gcgtgtaatc
    22981 aaattggtat tcatgggtaa tctctttttt tgtatctgta taaaacaagg attgcacggg
    23041 tacctttaat tctgtcaacc gattttctaa tgcttgacct tgctcctgga atgagtatgc
    23101 atttccgtca gttatatacg ttggcggaaa ttccttagaa acatggtcag ccacactagc
    23161 ttctttcaat tgctcactcg ttttccaatc ttttgttcca atcaacgacc acgcaacggt
    23221 tttgacgaaa aacttcataa atcgatcatc cgaatgctgc tgtgctgtta gcttcaaatc
    23281 aacaggtccg caataagaaa tggttccttt cagactatct tttggaagta gctgtttgat
    23341 tttcatttgt ttagcatacg catcattcgt ttgtatagct gcatattgca atgcgatttg
    23401 tgaacctgca ctatctccac caaaaaataa ttgttccatg tctagcaatt tgtcgtcttt
    23461 ttcttcagct aataaatctt tgatcagttc attcacttga agaacttgat tagggtattc
    23521 tgaatcaggc gctaattcat aattcatcgc aaccactgcg atgttggcgt ctgaaactaa
    23581 ttttgtcgca aattctttga cacccgattt atctccgccg acgaatcctc caccatgtac
    23641 ccaaaataga acaggaacag gccccttgct gttttttgga tagtagatgt cataagtatt
    23701 ctctttatac ttggactcat acgtcaaatc tgtttttttc gccacgtttt tttctgcttg
    23761 atcgaacgtt tttttatcag tgatttgaac agtatttgaa aacatatggt tgatcaagaa
    23821 tgctccaggt ctgggggaaa gctggaaggc aagaaagatt gcaaacgcga tcccaagaat
    23881 gattccgaag aaccatccga taaatttaag aattttcttt ttcataaggc tccctcagta
    23941 gttagtatta agcattctga ttataccata gtgttatttc ctatcaagct ctcctagatg
    24001 atttaatgat tttccccaaa cttcttctgc aatgcttttg acaaaagaaa ttttttccat
    24061 tgttcttcaa acaaaacatt ttcttcttct gttgaagcaa atccacattg aggacttaag
    24121 cacaaacgat ccaaaggcag atatcgtgtc gcttcgtaga tccggtcaat cacttgctgg
    24181 tgctcttcca tttcgccatt tttcgttaca gtcaaaatct aagacaacta atttatcttc
    24241 agagactttc tctaaaagaa caaaactccc agcacgttca ctatcgtatt ctaaataata
    24301 ggcacggaca ttttcttgag taaaataatg ggtctgctac gtcgtcatcc cccctgatgc
    24361 cgccaaaatc gaataataat ttccgcgtca aacatgtata tttagtgttg aatcatttga
    24421 gaatatgaaa ataaaaatct ttttgatcaa tcagttaatc aaattgaaat aacgaaaaat
    24481 ctgagacaga atgacgttca ttctgaccca gatttgattt tgcacaactc aacagtcctt
    24541 attggcaaat cgccaaaata atgtagacta actaaaa
//
LOCUS       DACDRN010000037        24283 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDRN010000037 DACDRN010000000
VERSION     DACDRN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.037"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(56..1351)
                     /gene="purB"
                     /locus_tag="IWJ80_001717"
     CDS             complement(56..1351)
                     /gene="purB"
                     /locus_tag="IWJ80_001717"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP9461433.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1364..2497)
                     /gene="purK"
                     /locus_tag="IWJ80_001718"
     CDS             complement(1364..2497)
                     /gene="purK"
                     /locus_tag="IWJ80_001718"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP9461434.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2481..2969)
                     /gene="purE"
                     /locus_tag="IWJ80_001719"
     CDS             complement(2481..2969)
                     /gene="purE"
                     /locus_tag="IWJ80_001719"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP9461435.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3385..4692)
                     /locus_tag="IWJ80_001720"
     CDS             complement(3385..4692)
                     /locus_tag="IWJ80_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP9461436.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4695..5276)
                     /locus_tag="IWJ80_001721"
     CDS             complement(4695..5276)
                     /locus_tag="IWJ80_001721"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP9461437.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5392..5489)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5848..7140)
                     /locus_tag="IWJ80_001722"
     CDS             complement(5848..7140)
                     /locus_tag="IWJ80_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9461438.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7137..7760)
                     /locus_tag="IWJ80_001723"
     CDS             complement(7137..7760)
                     /locus_tag="IWJ80_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461439.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7763..8599)
                     /locus_tag="IWJ80_001724"
     CDS             complement(7763..8599)
                     /locus_tag="IWJ80_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9461440.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8616..9503)
                     /locus_tag="IWJ80_001725"
     CDS             complement(8616..9503)
                     /locus_tag="IWJ80_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9461441.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9519..10802)
                     /locus_tag="IWJ80_001726"
     CDS             complement(9519..10802)
                     /locus_tag="IWJ80_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9461442.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11485..12168)
                     /locus_tag="IWJ80_001727"
     CDS             complement(11485..12168)
                     /locus_tag="IWJ80_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9461443.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12186..12803)
                     /locus_tag="IWJ80_001728"
     CDS             complement(12186..12803)
                     /locus_tag="IWJ80_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP9461444.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12813..13637)
                     /locus_tag="IWJ80_001729"
     CDS             complement(12813..13637)
                     /locus_tag="IWJ80_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP9461445.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13630..14190)
                     /locus_tag="IWJ80_001730"
     CDS             complement(13630..14190)
                     /locus_tag="IWJ80_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461446.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14364..15320
                     /locus_tag="IWJ80_001731"
     CDS             14364..15320
                     /locus_tag="IWJ80_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9461447.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15625..16581)
                     /locus_tag="IWJ80_001732"
     CDS             complement(15625..16581)
                     /locus_tag="IWJ80_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP9461448.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16685..17287
                     /locus_tag="IWJ80_001733"
     CDS             16685..17287
                     /locus_tag="IWJ80_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9461449.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17375..18874)
                     /locus_tag="IWJ80_001734"
     CDS             complement(17375..18874)
                     /locus_tag="IWJ80_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP9461450.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18890..19414)
                     /locus_tag="IWJ80_001735"
     CDS             complement(18890..19414)
                     /locus_tag="IWJ80_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461451.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19712..20461
                     /locus_tag="IWJ80_001736"
     CDS             19712..20461
                     /locus_tag="IWJ80_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP9461452.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20452..21237
                     /locus_tag="IWJ80_001737"
     CDS             20452..21237
                     /locus_tag="IWJ80_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9461453.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21206..21922
                     /locus_tag="IWJ80_001738"
     CDS             21206..21922
                     /locus_tag="IWJ80_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9461454.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22024..23043)
                     /locus_tag="IWJ80_001739"
     CDS             complement(22024..23043)
                     /locus_tag="IWJ80_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP9461455.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23062..24120)
                     /locus_tag="IWJ80_001740"
     CDS             complement(23062..24120)
                     /locus_tag="IWJ80_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP9461456.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7197 a         5224 c         4226 g         7636 t
ORIGIN      
        1 ttttttggtt atttttgcta ttctatttta attgaatttt gcaactcact cttctttatt
       61 ccaaacctac tcgtttgaat atcacgtcaa cgtttttcaa atgatgatga taatcaaagg
      121 catcgtctat ttcttcttta gataataccg atgtgatggt tggatcttct tctaacagcg
      181 ggcggaactg cacttggttg tcccaagcgt acgcagtttt tggctgaaca agatcataag
      241 cttcttctct tgtcattccc ttatcgatca atttcagcat aacgcgctgg ctataaatca
      301 aaccaaatgt tgcagacata ttccgcttca tgttttcagg aaagaccgtt agatttttaa
      361 cgatattgcc aaaacgattt aacatgtaat tcaataaaat cgtggtatca ggcaagatga
      421 ttcgttctgc tgaagaatga gaaatatctc tttcatgcca taatgtgaca ttttcatagg
      481 cagtgaccat gtgtccgcgc accactctgg ctagaccagc catattttct gaaccgatcg
      541 gattcctttt gtgcggcata gcagaagaac ctttttgacc tttcgcaaaa aattcttcta
      601 cttcacgtgt ttcggatttt tgcaatccgc gtatctctgt tgcaaaacgt tcaatacttg
      661 tagcaataag cgccatcgct gagaaatatt cagcatgtag atcccgaggg agaacttgag
      721 tagaaatttc ttgtggacgg atccccaatt gtttacatac atactcttca ataaatggcg
      781 ggatatttgc gaacgttccc actgctcctg agatttttcc tgcttctact ccttttgccg
      841 catgttcaaa tcgttcgatg ttccgtttca tttctgaata ccatgtggca agtttcaatc
      901 caaaagtagt tggttcagca tggacaccgt gtgtccgacc catcatcact gtatatttgt
      961 gttcttttgc tttttcgcca atgatttccg taaaacgttt caagtcttcc cgtaaaatat
     1021 catttgcttg cttgatgaga tagccataag cagtatctac gacatccgta cttgttagac
     1081 cataatggac ccattttctt tcttctccca atgtttcaga aaccgctcgt gtaaacgcaa
     1141 cgacatcatg gcgtgttaat ttttcgattt ccaagatccg atcgatatca aatgatgcat
     1201 gctcacgaat ttttttcaca tcttcttgcg ggatttcacc aagttctgcc caagcttcat
     1261 ctgctaaaat ctcgacttcg agccacgcac ggtatttgtt ctcttccgtc cagatattcc
     1321 ccatttcagg gcgtgtataa cgttctaaca tgttttttct cctctaatcc caaatattcg
     1381 tttgtttgat ttcttccagc gtttgctgga tgtcttctgt caagatagtg atatgtccca
     1441 tcttgcgacc tttttttgct tgtttttttc catagaaatg gaattgccag tctggcttat
     1501 tctcaatcaa gcgatagctt tccagcagtt gttctcccaa aacattgacc attactgcat
     1561 tgcttatcaa acgtactttt tctcctaatg gccaaccaca aatacctcga atatgggcat
     1621 cgaactgact catagaacaa gcttcaatag aataatgtcc agaattgtga ggacgaggag
     1681 ctaattcatt cacatatagc ccgcctgttt ttgttaaaaa catttctacc cctaaaacac
     1741 ctgaaaggct cactgcttct gcaatcacac gggcaatacg ctcagcctcc tcgatcacat
     1801 cctgatcaat cgcggctgga gcaatcgttt catgaagaat attgttgtgg tggatgtttt
     1861 ccacgaccgg aaaagtcgtg aaatctcctt gaccatttcc tgccaccatg atcgaaatct
     1921 ctttttcaaa cgggatccat gcttctaaaa cacaggttcc ttctcttaaa aggtccatgg
     1981 caggagctag atccgcccga cttttcaaaa catactgccc cttgccatca tagccgcctc
     2041 gagtggtttt caaaacacaa ggatagccga tcccgtcaat cgcatcttgg atatccgtcg
     2101 gactgacgat cgttgcataa ggagcaatca cgatgttatt cgtttccaaa aaagattttt
     2161 ctaacaggcg gtcttgcgta atcgcaagca agtctgtccc ttgaggaata aatgacatgg
     2221 gtaaaatcgc atttaacgcc tctacactga cgttttcgaa ttcataggta atgacatctg
     2281 ttcttctggc aagctctttc agcgcaaagg tgtcatcata gacactttca atatgccaat
     2341 ctgcaacttg tgcggctgga caatcgctca caggatccag cacacctact ttaaagccca
     2401 tttcttttgc actaatcgcc atcatacgac ctaattgacc gccaccgacg atcccgatcg
     2461 ttgaaccagg caacaaaggt ttaaccaagt tgatcactac tttctataac agttttagcc
     2521 aacattgaac gtctttcagc caatttagcc tccaattcta aatcatacat ggaaagcatc
     2581 tgtatcgcaa gtagtccagc attaattgct cccgcttttc cgattgctgt agtagctaca
     2641 ggtactcctc caggcatttg gacaatcgaa agaagcgaat ccaaaccatt taatgttcgt
     2701 gattggaccg gtacaccgat aaccggcaaa gttgtttttg atgcgaccat acccggcaga
     2761 tgcgcggctc cccctgctcc tgcaataatg attttgattc ctttttttcg agcgttttgt
     2821 gcatattcaa acatcaaatc tggggttcga tgagcagaaa cgaccttttt ttcataagac
     2881 acaccgaatt catccaatat ttgacatgct tctttcattg tttcccaatc tgaagtactc
     2941 cccataatca cagagatttt tgctgtcatt gtttttcctc ccagtttttc tacattcata
     3001 ttttaacaat cacttttttt gatgtcaaag aaatttcgaa tgttaacaaa gaaaaacatt
     3061 ttattgttcg tgtttattaa agataaaaaa aaggaatgtg acaaaagtta ggtcacattc
     3121 ctttttatgg ataaacggtg ttctcctgcc aactcctcgg tcacaaggaa caatattcaa
     3181 caaacatgag aagctttttt ttgaaattgt tccttgtgac tcggagatga acggcaggct
     3241 cacgacctct tccttcaacg gtgttcaatc agctgaccct cggtattagc tcaaacaaac
     3301 ggcaaaatac gaggaactat ttttccgttt gtttctctaa caatcaggtc aaaacgctga
     3361 tttcacaacc tttcttttgc tctattactt cttgaaggct tcttccaata tttcttcatc
     3421 ttttttcaat ccattaaaga tcaaattcaa tatgatagcc gtaatgctac tcattacgat
     3481 gccatttcca gtgaacattt gcaatgtttg cggcatttga ctgaataatg tcggcgtaac
     3541 attgaaaccc agcccaaatc caacagaaac agcaacaatc aatagatttt tatcattgct
     3601 gaaatcaact tccaacagca tacggattcc ttggactgcc accatcccga acatgactaa
     3661 cattcctcca ccaagtaccg gctcagggat gatttgtgct aacgcaccga ttttagggaa
     3721 taatcctaaa acaatcaaga agaatgcaga gaaatagatg ggttttcttg ttttgattcc
     3781 cgaaagttga accaatccga cattttgaga aaagccagta taagggaatg tattgaaaat
     3841 accgcctaag ataacggcaa gtccttctgc acgatatccc tttttcaatt cttcttctcc
     3901 aacatttttc ccagtaatgt caccaagtgc aaaatataca cctgttgatt ctaccatgct
     3961 gaccatggaa atgatgatca ttagtaaaat cgaccatacg tcgaaagttg gtttaccaaa
     4021 ataaaagggt tgtggaacat ggaacaacgg cgcatctcca atcgcgctca gatcaatcat
     4081 tcccatgaaa gcagctaaga tgcttcctcc gactaaacca atcaataccg cgattgaacg
     4141 aatgaaccct tttgccagca tttgcacgcc tacaattagg agaatcgtca caaatgcaag
     4201 tagcagattt gttttgtcac caaaatcagc tgctgttgca ttacctccac ccattttctc
     4261 gattgctaca ggaatcaaag tcaaaccaat aacggtgata accgttcctg ttactatagg
     4321 agggaatagt tttttaattt tagaaaagac accagaaact aacacaacaa agatcccaga
     4381 agcgataata gatccgtaga tagctcctac tccttcattg gttccgatca agatcagtgg
     4441 agctactgct tggatcgcac atcctaaaac gacaggcaag ccaattccga aaaatcggtt
     4501 aactgtcaat tgcaataatg tcgctaatcc acacataaag atatcaattg aaatcaaata
     4561 agtcatctgt tcttgattaa agcctagtcc tgtaccgatc aaaagaggca ccgctactgc
     4621 tcctgcatac atcgctagca gatgctgcag ccctaaaacc gcagcttttc cattttgtgt
     4681 ttctttttcc acgattatgc gtcctcctca agaaactcaa cttgtccatt cgtcaaggaa
     4741 gcaatacgcg caagagaaac gactctcaat cccatttctt caagtaactg acgtccatct
     4801 tggaaagact tttcgatgac gatcccgatt ccttctactt gcgctccagc ttgctggcat
     4861 aattcgatca atccttttgc cgcttgtccg tttgccaaga aatcgtcgat aatcaatact
     4921 ttgtcttctg aagataaaaa tttacgagaa atagaaatcg tacttgtcac ttgtttcgta
     4981 aaagaataaa cagaagctgt caataattct tcatccatcg tcaaactttt tgcttttcta
     5041 gcgaagatca ttggaacatc taattctctt gcagcaaata aagctggtgc gatcccagaa
     5101 gcttcaatgg tcacaacttt cgtgattcct tgttctttaa atacttctgc aaaccgtttt
     5161 cccatttgtt ccatcaatac agggtctact tggtgagtga cgaagctgtc gacttttaat
     5221 acgccctcgc ctaatatacg tccgtctttt tgaatacgtt caactaattc tttcacttta
     5281 tttgctcctt tatcatttat taataccgaa ataaccgctt ttgtgcaaaa acctactatg
     5341 tttctgttaa agaaaaacac cctccacttt atcataagaa aagaagggtg aaatagccaa
     5401 tcttttactt atagtccagc atttacggta ctggctagaa actgtcatcc atattatgac
     5461 gatatataag taggtatgta aaactaataa tagtgtaaat tcattttctt gcaactgttt
     5521 tgcacgtgaa ggcagtttgc catatttgaa taaaaaatgt tgtttcaaat attgaaaaca
     5581 gtctaacact actttctgaa actgacaacg tggtttactt atttttttgt aaaatctaat
     5641 gatttttaat gcattattcc tttttgttcg ggtttaaata gttattttta ttttctccta
     5701 tctaaaaaag cagcccacat aaaaagaagg catgacaaaa agcatgtcat agcccctttt
     5761 tccgaataaa tagtgttcaa aagacagcct atcggcaata aggcaagatt ttataaacta
     5821 cgggaagtaa tttccgtaaa tctattctta ttcttttaag ctgacaagct cgtcaatcac
     5881 tactgctttt cctgaagcaa tagaatgatt tgctgcgatt cctgtcaaaa tcgacaaaat
     5941 accatctacg tgagttgctg cccgattcca cggatcttct tctttttctt caccaaatag
     6001 atcatctaat aaacgcggat ctcctccgcc atgtccgccc gcttcttgtt cgacgattac
     6061 ctcatatggc tcaccgaaca tcggatagac atggatggac tgaagaacgg ctgctccttc
     6121 atccttttta tctccacctg cattgatgta agacttttcg atcactttca tttcgatccg
     6181 tcctttgctg ccattgaaag aaacattgaa tccttcccac ggcatatacg cgtttagcga
     6241 atacgtcaaa atcgcaccag tttcatattt gaccgttacg cctagtgtat cttcaataga
     6301 gatatcatcg gaaaatacac tgcggtcacg aatatatcca ctgtcttttt ctgcttctaa
     6361 atacatttct cgcaattcct cattggctga caggtctata gcaaatggat cagctttagc
     6421 tagttcgctt ccatagcatc gactgtaaaa tatttctact ccgcgtttct ccgcattatg
     6481 catcccataa aagtttaatc caccgaatgc aaataccttt tggggacggc tgttcaacca
     6541 aaaattcact agatcaaaat gatgggtaga tttatgaaca agaagcccgc cgctattgtg
     6601 cttatttcga tgccatcttc tgaaataatc tgccccgtgt tgcgtattta acagccattc
     6661 gaagtggata gaaaacacat caccaatcac atcactttct atcagttcct ttatcttcgt
     6721 gtgatacggt gcataacgat aattaaaagt gactcgcagg cttcttcccg tctgtttttg
     6781 gaccgttatt atttcttgag ctttttcctc atcgatagtc agaggttttt cgcaaataac
     6841 atcatatcct tttttcattg cacgaatgat ataatcatga tgcgttcgat ctactgacgt
     6901 aataatgact tcagccggcc gtacgtcatc cagtagttgc tcaaattgtg atggcataca
     6961 tgtctttact tttttctgtc catacttgtt cacaagtatc cgatttgcat aatccatcct
     7021 tgtttgagat acatcacaaa aaccgactag ttcacaagtt tgatgatacg tagaggcaat
     7081 tgcctcataa aaaaaacgcg ctctcccacc aagtccgact tgtacatatt ttttcttcat
     7141 ttaaagtcat tctccttttc ttttaagcaa aatactgtga caaatggcag ccctggatac
     7201 agtaatgcag tgagtaaaaa atcatgctgt atacaccaga acagtccata caaaaggcat
     7261 aaataccaca gaaacccctt gacgtttttt gacaacagcc agatattcct tttgatcaaa
     7321 taaacaagac tatctgcttt atcttctttg cagatatcaa ttagcgtact aaaaaaacta
     7381 tgggatacaa aaagcaacag acacaataga aaaggaatca gataccaagc tccaatcttt
     7441 cccactaata aataccaatc aactgcaaaa ataatgacaa acagctgaaa caacgtaatg
     7501 atgaaacaca tccgtttgat ttttcctttc ctccaacata cctttttttc tgccaagtaa
     7561 tggacagata gataataaga aggatacatc atcaaaaaat aaatcagata gacaacacga
     7621 gcagacagca ctaatcctaa agtgaacaaa catcctagaa ctaaagcgaa aagagcatta
     7681 ctgataagac tggttaaaaa aatccgacag acatagtagt tgagcttgta aagaaacatc
     7741 tcttctattg gctttttcat attcagccct tcagaccgct tgtactgatc ccctggacaa
     7801 gatatttatt gaagaataag aaaatcagaa aaaccggtag cagcgacaac gtagacatcg
     7861 caaacatact cgcgtaagtc gtactagatc cagcgtctgc aaataatttg atggcaatcg
     7921 ataccgtgta agatctcggc ttgtttaaat aaatcaatgg tcccatgaag tcgtcccatt
     7981 tccaataaaa ctggatgatg atcgtcgtga tgatcgaagg tttcagcaaa ggaaaaataa
     8041 ttttggtaaa gacactatac ttgctgcatc cgtcaataat cgctgattca tctaactctt
     8101 tggggatgct gaccatgaat tgcatgatct ggtaaatgaa aaatgcttgt ccaaaaaaat
     8161 gtggtaaaat cagcggcaca tacgtcccaa ccaaattcag ccgattgtaa ataataaact
     8221 gcggaatcaa aatcacttgt gcaggaatca tcatcgttac taacatgacg acgaaccaga
     8281 atctttttcc tttgaaatcg attctcgata atgcgtaagc aatacaagtc gagctcagaa
     8341 ctgtccctat cgtcgaaact gcagagatga caaacgaatt cctaaagaaa gtaaaaaatg
     8401 aaattccagc aaaccccgcc catccttttg cgtaattttc ccattgaaaa gtctctggaa
     8461 tcaaattcag cgaaccactc ataatctctt gatccgtttt aaaagaacct gcgatcatcc
     8521 ataagaccgg ataaatcatg actagaccta atagcaaaat aaaaccgtat tgaaaccatt
     8581 tcgttactgt attattcatt tcttcctcct ctcattcaca caacgaccca tttttttgat
     8641 gatttgaaaa tcagaaaggt gacagctccc ataatcaaca gcatgatcca cgaaatagca
     8701 ctagcatacc ccatagaatt aaaagaaaaa gcttggttat acacataaag agcataaaag
     8761 ttcgtggcat cattcggacc accttgggta atgataaatg cctgcgtaaa agtcataaac
     8821 gccgcgattg tctgcataac caagttatac aaaatgatcg gcgaaagaag aggtaacgtg
     8881 atcttgtaaa acgtctgcca tttgttcgct ccatcgatct ctgccgcttc ataatacgag
     8941 cctgggatct ctttgatacc tgcagcaaag atgatcattg ccgaaccgaa ttgccaagca
     9001 gacatcaaaa caagagggat catgaccagt ttttgatcgc taaaccagga aagcgcaggt
     9061 gcgcctaatc gttccaggaa aatattcact aacccgtttt tagaaaacaa ctcccgccaa
     9121 actagagcga tagaaatact gccaccgatc aaactaggca cataatacaa agaacgaaag
     9181 aaagtcgttc ctttgatatt cctcgttaaa agataagcaa tcaatagacc aaagatcagt
     9241 ttcacaggta cagatgcaaa tacatagaga aaagtgactc ggatggagtt caaaaaacgg
     9301 gcatcactca gcatctttat ataattatcc aagccgatcc atgagactgg atttcccaaa
     9361 cgataatcgg tgaacgaata ataaagtgac aagatcatcg gtatcactgt tatcaataga
     9421 aatcctagga tgaatggaga agcaaatacg taaccgacca cattgtcttg atccaagaac
     9481 cgttttagtt tttctttttt catgccttcc tcctgctgct atcctagaat gtttgacgca
     9541 aactcataaa gactctctgc tgcttcttct gctgttactt cttcataaat gactttttca
     9601 actaaaagat tgtattggtc ttgaatttcc ggattcaaag cactatcaaa gacattcact
     9661 tcgccgttgt ccatatcgga aatcatttct aaataatcaa atgtcgcttg caaagttgca
     9721 tcatctgcac gagcgagctg ttctcgaata ttcgacatga ttggtacacc acgttctcct
     9781 tttaaaattt cattggcttc ttcactgttt tgaaagaaat caataaactt agcagcttct
     9841 tctttatggg ctgagctttt tgccatactc agcatttgcg aagaacgtaa agggatacca
     9901 gattctccat ctgcttttct gcgaggtgga ttaatcaact gaatctccat atcttcagga
     9961 gcccctgaca atatttctgt caactggtta cttgatagaa acaccattgc cgcttctcta
    10021 tccctaaccg cctgaaaaga cagatccgtc gtacttgcag ctccaccacc tggttctgga
    10081 tgggcccctt tgtctaccag atttttacgc agctggaaaa aatctgttag atagtctgga
    10141 ttttcaaatc ccaagctgtt agatagatct tcccgattat aaaaattcaa tcctttctca
    10201 acttgtggta tttgaataat acagccagct ataaaatctt cgaatcttga aatagcatag
    10261 attcctaatt cttcagtgat ttgatagctg atttgttcaa attcatccca tgtccaattt
    10321 gaagctggtt cagggatatt taattgttca aatatatttt tattgtaagc aatcgcaaac
    10381 gaattcaccc cgttagacag tccaactaat tgcccgtcgt aagtcgtcgt tgctaaatag
    10441 gattcatctg cattagaaat atcaattatc ccttgctcca catattcatc taatggttca
    10501 atctgattga tgtattgagg aaattgattg ttcagttgga aaatgtccca gatatcatct
    10561 gatgctgcta gtgtacctaa ttttgtgtaa tatccttcat aatcataaaa ttcataatcg
    10621 atcgtcacat ggggattttt ttcttcataa agctcgatta cttttactgt tgcatcgtgt
    10681 cggacttgcg aaccccacca agccatgttc agcgtgattt ttttgtcatc ttcttgccca
    10741 caagccgtca agaagaataa cataacactt aaagctaaga tgattctcat tcttttcttc
    10801 attctctgca cctctacttc cattgttatt tttcaatata ctgtattttt atacagaaca
    10861 tatttattaa acgatctttt tcaaggggaa taaaaaccaa gacttgcctt atcgttctgc
    10921 ctaggatgaa caactcaata ctttcaagaa aaattagata gtgctgattc ttctttcatc
    10981 gctaaaaaca cttcttgctt caatgatggg aggataatta cgcaaagaaa aaaggatatc
    11041 agacaactgc ccaatatcct tgacatagct aaataagcat gcttcttcgt gtgcgctctt
    11101 ccctaaaaaa cagaaaaata actagtaatt ctgtatgaaa ttaggtacaa tatacacgtc
    11161 gatgcacaag cattgactat ggcaacttga ttggtcagtt cccgctgact tttcaagccc
    11221 cgacaattta gctgctacta aattgtcggg tgcctttttt agtttttatt cgtttttcat
    11281 tctatagaaa gcgcttcaat aaatcaagac atttttttgt tagaatgcta ataataacaa
    11341 aaggttttaa ctcttcttat gtacattaca ggtaaaaaaa caaaaaaatt tctctttttt
    11401 ttgaattctg aacaaatagt actggagtac aaaagacgag gaagagccac tatctgctct
    11461 tcctcgtctt ttgattctca cctgttattc atgattcttt ttctcagttt cccgatattt
    11521 ttccatttta cgactaaaaa gctctccgct acttggtggc gtatatgtga tggcatttgc
    11581 tcctgcttga atggtctcag caatactttc ttccgttgga ccacctgtcg caatgatagg
    11641 aagttcagga tattttttac gaaaatggcg tactgcttct gcagtttctt tacctgcact
    11701 gatattgata atcgaaatac cagctgctaa cttttcatct atttctgtat atttcgacgt
    11761 taccgtactg ataactggaa tatcaactac tcgacaaact tcttttactg tctctaaagg
    11821 cgtaggaccg ttcaaaacga cccctaacga accttgcgct tctgcgaaca tgctcatgta
    11881 ggaagaacga gcaccttgtg tcaaaccacc acctacaccg gagaaaactg gaatgtcagc
    11941 tgcctcaata atgcttttag taatcgctgg atgaggtgta aaaggataca cagcaattac
    12001 tgcattcgca tttgtattac gtatgatcgc aatatccgta gtaaagatga tcgaacggat
    12061 caattttcca aatatcttga tcccgcttgc ttctaaaatg atttccggta ctcttacgat
    12121 atcttttctc aattccgtca tgatttctgg cgtaaattgt tctgccatca gccacttctc
    12181 ctttgctatc tccggtcata ctgattcaat tgatacgtct cgatgacctg gatgattttt
    12241 tgcgcataac cggggtctgt cgcataacct gcatcttgca atgcttgtgc ggcttcctga
    12301 tagttggtgg ccgtaatcac tttttcatac agcgcaggat tccaatctac tccttggacg
    12361 aacagttgtg tatggtcatc catggactgt tcccatgaat catacacttt gaagtctcct
    12421 tggatagtta tccattcttc gttaacaaat tcctttgttt ccaggcttac tttcttctga
    12481 tccccataag ccttgatccc gaaaagatta ttgtatttgc tagccaaggt actttttccc
    12541 caatttgatt ctaatatagc ttgtcctaaa atgatactag gaagcactcc ataaccatct
    12601 tgtagttctt gggcatgcgg aacaagccgt tcaataaatt gctgatggga ttgcgcttca
    12661 ttttcttgct gattatcagt aaaaggagaa gacagattct tcaaggaaaa aacaaaggct
    12721 aagagtatca ctgctacacc ggcaaccact gctggccaac ggatattctt gcgcttttgc
    12781 tttttccact tttttttcgc catgatcaat tcctactttt catttaattc attcagatat
    12841 tcttctagcg tcaattggtg tttggtgata tactcagcgt tttctttacc gacataacgt
    12901 aagtgccaag gttcatatgt gatcttagtt atatcttcgt gtccttttgg ataacgaacg
    12961 ataaatccat actgcgcagc attatctgcg atccatttag ctccaggctg atctccgtaa
    13021 cttgcatcca atacagtaga aggataactg ttgtaccagt tttgatcgac gacatcgact
    13081 gccagacctg tgtggtgttc gctgtatcct ggctccgtaa tcgtttcttt agtaatcttt
    13141 gtcgcctcct cttctgatac accttgacga ctcatcacat cttggacatt ttgttcgaaa
    13201 acttgttgtt gggatgatac agaccggtaa gctgagacca tcactaatgg aaatccagct
    13261 gcttcagcag ccttggcaaa tgcttcatat tctggagcaa tgcgtttgtc gatcaagtat
    13321 ccgttactta atgatgccaa ttgagtagac tcatcaattt ctttttctaa tttgtgatct
    13381 ggaccaacca aaactagctt ccaatcgtct gttttaacat ctggcaattg tttggacgta
    13441 cgatcttttg tttctttgtc ctttaccttg ctgctggttg tttgactgtt cttttcttta
    13501 ctagcagttg ttttggtgct tgttcgttcg actgcttgat tgccggcttg aacatgcgaa
    13561 tcatcatcgc taaaaataac atagcccatt gctgcgatca taatcacaac tgctccaaat
    13621 cctaaaaatt tattcacttg aaaaatcctt tcgttgttct tcacgttccc cattcgcctc
    13681 atgaacgtaa gtttcttcca gattttcatt cacccaatcg agaagttcga ttttttctcc
    13741 ttcgatctgt ttttgaacat ctgctggcag acgaaaattc aaaatatcat catagcccca
    13801 ttcatcatat tgaatactta ctttcaagtc aaggtatcta ccagcctgct gattattttt
    13861 caaagggatc agctcgactt ttgctgcttt gctgctaagc catttttgcg cgatgcgtgt
    13921 tttgatttgc ttgtactctg caacggcaac gtttcgtttt tcgggaaaaa taatcgtttg
    13981 acgcaaaatc ttttgcgtta gcatccattc ataatcagtg aaaagatttt ttactttctg
    14041 catcgcttca gatggcagat ttttctcgcc atctttccat ttttgccatt gttctggact
    14101 gatcagcaag tattcatcta aaaaattttt ttcagtttca aactgttcta ataatacact
    14161 cataataaga tcaatatata cttgatgcac gtttatcccc tccttttaac tattctactt
    14221 aagttttgat caaaaagcac ctcttaaatc gctgctttca ttttttttaa tgtttcaagt
    14281 cctctcctct ttattcttcg tgatttttgt tataataata gacagacaag aaactcaatc
    14341 caactatgaa ggagcgtttt cagatgaaaa aatatcaatg gggaatcgtt ggtcttggta
    14401 ccatcgcaca tgagtttgcc gaaagcttca atcaagagac gagcgaacta acagcagttg
    14461 cttccagaac ttcagaaaaa gcggaaaact tcgctcaccg ttacaatatt ccaaaagctt
    14521 acggaagcta tcaagaaatg ttagacgatg ctgaaatcga catcgtgtac attgctgtac
    14581 caaatagaca gcatatcgac catatcttgg cagcattgga agcaggcaaa cacgttctat
    14641 gtgaaaaagc catcacgatg aataaaaaag aactggctga cgctatgcgc ttagcagaag
    14701 aaaagaattt gatcttagca gaagcgatga caatcttcaa catgccactt tatcagcagt
    14761 tacgctcaat aatggataca ggaaaactgg gggctttaaa aatgatccaa gcacctttcg
    14821 gcagttacaa agaaccagac cctaaaaacc ggttcttcaa tccagaatta gctggaggag
    14881 ctctgttgga tatcggtacg tatgctgtat ctttcgctcg tttctttttg agctctcaac
    14941 ctgaagtagt tgcctctaca atggttcctt ttgaaacagg cgtggacgaa caatccgtca
    15001 ctatcttgcg taacaaagaa aatgaacttg ctgctgtcag cttgactttt caggcaaaaa
    15061 tgccaaaagt cggtatcgtt gcctttgaga acggttatgt gacgattgct gattacccgc
    15121 gtgctgatcg tgcggagatc ttatttacag atggtacaaa ggaattcatc gaaagcggga
    15181 atacatctga agcaatgaat tacgaaataa agaatatggt acacatgatt gaaggtgact
    15241 tgcccaatcg ctctctcttc ttgacaaaag atgtcatcga aatcttggat caaatgcaag
    15301 ttctatggaa aaatatgtaa ttttaaaagg ttgtgaaaaa gctgttctgc atcaagtaat
    15361 aagaacagcc aaacaaaaat agctcctcct atttttcgtt tggctggact tattaccgca
    15421 gaggaaagct tttgaacacc gtttaagaaa gaggttgtga gcctaccgtt cagctccgag
    15481 tcacaaggag caatttcaaa aaacagcttc tcatgtttgt tgaatattgt gacttgtgac
    15541 cgaggagttg acaggagaac accgtttaaa gaagaggttg tgaaatcagc tttttgacct
    15601 gaatattagc tgaattcaca acctctattt ttggattttt tgttgggaga attctgcctt
    15661 gatttcaata aaacttttaa aagactgcaa aaactggaac aattctgctc gatcctcgaa
    15721 ttcagggcga ctagtcggca gaggcatttg ccgatacaat acataaactt cttcaatacg
    15781 tgttaaaatt tgtctgccat catttgactc tgaaaatgtt tctgcagtat aaatcaataa
    15841 accatagata tgttttccat aataagctgg ttgctggatt ctctctagat tttccagcat
    15901 gtctttaatc acattggcct gtgctcttct catagagaag taagtttcgt agtatagcgg
    15961 ctgtctgagc cagtagtttt cttgatgttc tctggcatct tcttggcttt cgcgaatgaa
    16021 tgtcagtaaa tgttcgcatt tgatctgcac atcttcttta ttttctgata aaagaaattc
    16081 tgccatttgt ctcaaaatat ttcgaaattc tttttcgatc tgtatttggt tttccttcag
    16141 ctgcttcgta cgatctggca tgtaaacatt tgcaagcaaa gcaaatacta atccaatcgc
    16201 cattaatccc gcttcattgc cgatcagctg ccaagacatg ttttcctcca ctaaataatg
    16261 agtgaccaaa accgaattta ctactatccc ttcggttaat ttgaagcgag aggcaagcgg
    16321 gatgaacagg aataaaaaaa gactgaagcc aaaaatagta tacccaacag caaaaaaact
    16381 gaacagtgct gttgccgtag ctaatataaa ggctgcgatt cgattcatgc ccgtacgcaa
    16441 tgtagagcgc ttggtatttc ctacacttaa taatgcaatg atccctgcag ctggccaata
    16501 caacagcgat aagctacccg ctgtgaccat cgaaaagaag acactgatca ctgttttgac
    16561 tgttcgcatt cctatacgca tttacttccc ccttttttat tttaattata cttgaataat
    16621 gaaaaactag ctatcagaca aaagtttagg tatgatagta aaaagaccaa aaaggagaat
    16681 ctctatgcaa tttggccgtt ttcgactagg tatgcgtacg atcaaaagtg ccttagctgt
    16741 ttttttatgt atcttgtttt ttcatttgac tcaacgagga atcccgatga ttgctgcatt
    16801 atctgctgtt ttttctttgc gtcaggactt gactacaacc gtttcttttg gaaagtctag
    16861 gatcatcggc aatacattag gtggtacatt agctatcatt tatttttttg tcaaaagcta
    16921 ttttcacaat gattttttgg tagaattgat cttgcttcct attctagtta ttattgtgat
    16981 cgtcgtgtca gatggtatta acaataattc cggtatcatt tccgcaatag ctacgttgtt
    17041 gctgattgcg ttgagcatcc ctcaaggcga atccgctttg tatgctatcc aacgtgtact
    17101 agatactttt atagggacat tcattgcaat cggtatcaat ttctttcttc gtcctcctga
    17161 aacagaaaag aaggaagaac tatcagaaga tttggtcgaa ctccaaaaga aagaacgatt
    17221 gctaagaaca aatttacaag agatagaagc taagatccaa aaacaaaaga aaaatagcga
    17281 ggactaattt tcttttgcaa taaaataaaa aaatggagag aagaacttta aaaagtcttc
    17341 ctccattttt gattgaacgg tgaagtgtac tactttattc ttccttcatc gagttttgtt
    17401 ctgcctcttt tggcgcttca tacatattta cttggctagt agtcccacgt gtcgacaaat
    17461 tctctacgat ttccttcaag tcaagtcctg ttgtcgcttt tagtgtttct tgagtagaag
    17521 aaagcaaatt tgtggcataa tttgtcactc ggttcgctcc actggtttct cctgaactgc
    17581 ttcctgtatc gacaacagag attttttcga tatttcctag cggctgtgct gcttcgcgca
    17641 tcaattgcgg tagcatatcg ataaccatac tcaagacagc tgcttcgcca tattccttga
    17701 aggcattggc gatcttttgt tttgcttcgg cttctgcttt tccttttgct agtgctgctt
    17761 cggcttctgc ttgtccagct aaacgtgtct tattggcttc cgcttctgcc agtgcttcca
    17821 cacggaatcg ttctgcttcg gcttcagtca cttctttgac tttttgagct tgtgcttctt
    17881 gttctttagc gtaccgatcg gcatctgctt ttttcttcac ttccgaatca tattgctttt
    17941 cacgacgtgt gatttctttt tcttccagtt cgatttgttt ttgacgttct acgattttaa
    18001 tctgcatttc ctgttcgatt acttgttgtt gtgctcgggc actttctaaa ttgtaggctt
    18061 ggtctgcttt tgctttagca acatcttgtt cttgtttgta ggttgctaat ttcagttctt
    18121 tttcttttaa tgattctgcg atctctgttt gtcgctgtaa ttcagctgct tgtgattctt
    18181 tttctgcttg agcttttttg atacgcgttt cttttagagc ttccgcttct gctatatctg
    18241 catctctttt gacttgggcg attcttggtt tccctaaaga gtcaagataa ccgtttttgt
    18301 ctctgacctc tttgatagtg aaagaaacga tgatcaaccc catttttgca aggtctacag
    18361 aagcgacttc ttgtacgctt tgactgaact tatcgcgatt ttgatagatt tcttctacag
    18421 tcatagaacc aagaatcgag cgcaagtgtc cttctaatac ttctcttgct tcattttcca
    18481 gttcttctct tgtcttgcct aaaaactgtt ctgctgctgt agcaatctct tctacagatg
    18541 aaccgatttt gatgatcgaa gtaccatcac acatcaccgg tacgccttgt tccgtataca
    18601 cttcaggggt ggagacgtcc aatttactcg ataatagact gatccgattc gaacgttgaa
    18661 aaactggcaa gacgaatgcg ccacctccac gcacaatttt gattttgtta ttgctttcat
    18721 caatgtgcac atttttgctt cctaaataac ttccactgat gatcaacgct tcatccggtt
    18781 ttgctgtctg gtacttcgct acaaagacga tcagcaacat cagtaaaatc aaagctgcga
    18841 ttgcgatcgc gataaaaaca actggtattt ccatttaaca cacgctcctt taagctttta
    18901 tcatgctttc ataaggcatg acataacaga ttctttcctt gatctcaatg atcaatactt
    18961 tcgttccccg ctcagctgtc tgttcttggg ggctgtaaaa acaagctgga cgactaatgg
    19021 aaccggtaac acttttcaac tgaatttctc ccatacctcg tacagggatc ggtgtgatca
    19081 caacagctac ttgtccttcc agagtttttt cagaagacga catcgttgat tcggcatgtt
    19141 tcatcggcat caatatatag aaattaagca aaaataccaa cactatcgat atgcctccac
    19201 tagcaataaa taaaaataaa gatgattgat ctgttaaacg ctcacctaga aaaccaaata
    19261 agcttgtgaa agttaaccaa gggataataa gcatcggatc aagcgggcca tcaaaatcga
    19321 atacatctcc aaaaataatc aataggactg ccaatcctgc acaaatcagc aatgtatacc
    19381 aataaattgt ctcaagtgcg attcccccta tcatatacac gcctccttcc atttctacgt
    19441 ctatcatacc aatgtttgaa aattctgtat agcttctttt accgactcag tggtgttttt
    19501 tataggaagg ttgtgtttat cgttgacaat ttgttaaatt tttgttttca tgttaaaatt
    19561 ttcacaataa attctttcga ggtagcctat gtttcctttt tttaagaaaa aaccattaaa
    19621 gctccagaac acgatcatcc ttgtcgtttg tttctccaca ttcgtttctt tgattgtttc
    19681 tgccatattg atcaggaatt ttattctttc gatgaaccga tctagtgtgt cagataaaat
    19741 ctctagcatt gctaaaatcg ttgccaatga taaactcatc caaaaaaagc tgcagactgg
    19801 tgacccgaca catgcgattc aaggaataac aaatgaaatc atgattgcat cgaatttaga
    19861 ttttatcgtt gtcatggacc ctgaaggcat tcgatattct cacgcagacc caagttacat
    19921 cggtcttcct ttctcaagtc atgaagatgt gaaaaaagtg attggaggac aagaattttt
    19981 ctctgaaaaa gaaggaatgt tagggttcgg cttccgctat ttcgtcccta tttattatga
    20041 tcaagagatt attggtgcag tttgtgttgg cttaacaaac aacacgattg acaatcaagt
    20101 caaagatgga caaaaaatga ttgttttttc attgattatt ggacttagcg taggcatcat
    20161 aggcgccgtt tttcttgccc aaaaaatgaa aaaagtactg cttggacttg aaccacaaga
    20221 aatagcagaa cagttaagtg aaaaaacgat tatcgaaaat gaagtagatg aaggaatctt
    20281 agccatctct tctgataaaa ctatcctctt gatcaataca cctgcaaaaa acattttcta
    20341 ccaaacagag cagctattgg atttcgaagt aggaaaaaaa tcagtgataa tctatatgac
    20401 gttttgttta aagagacgat tgagcatcaa acgaccgttc ggactcaatc catgtattta
    20461 aatgggatag aaactatttc tagcgttaca cctcttttta gcaatgaccg ttttttagga
    20521 gcagtcgcaa ctttacgaaa tcgatcagaa gtaatgaaat taatggagga gcttgggggg
    20581 acgaaacaat atataaactc catgagagct caaacccatg aattcatgaa taaacttcat
    20641 gtcatttctg gtttgattga tttgaagaag tatgaagaag tcacccattt tatccagcag
    20701 ctaaatcaaa attataaaaa agagattggc caagtctcaa gtctgatcaa agtacctgca
    20761 attgccggtt ttttattagg caaaataaac gaagccaaag aacaatctgt tgaaatgctg
    20821 ttagaaagcg attcttttat ccctaaaatg gagatggatg actccatcca ttcacttttg
    20881 caagtgttag ggaacttact tgataatgca aaagaagccg tgctacaaaa acatgaaaga
    20941 aacggacagg tccggattca tttaaactat gacacagaag ctgaagtatt catcgtgacc
    21001 gtctgcgatg acggtgtcgg gatatcagaa gaaatctcag aaaaaatatt tacgctaggt
    21061 ttttcgacga agggaatcaa tcgtgggttt ggattgaatc tcatccattc aatcgtcaaa
    21121 cgccacggtg gatttttaga attttccaaa caaacaaatg gcggtacaat cgtctatcta
    21181 gaattaccaa aaaaggaaga tcaaaatgat gacaaatgtc ctgataatcg aagatgatcc
    21241 aatggttgca gaattgaatc gtacttttgt ggaaagcatc cctaatatga acgtaatcga
    21301 aagtttcaga aatgtagaag aaggaaaaaa atatttcaga caaaaaaacg cagatctgct
    21361 gctgcttgat gtatacatgc ctggcgaaag tggtatccat tttttatctt ggcttagaca
    21421 gcaaggaaat gatatcccag tgattttgat cactgctgca gatgatatcg aaaccataaa
    21481 gaatgtgatc cgctacggtg ccttagatta tttgatcaaa ccgttcactt tgcaacgttt
    21541 tcagctggcg atcgaaaagt atcttgcttt ctctcagaca atccaaacga ctgaaaaagc
    21601 cgatcagaaa ttgatcgatc aagtattcaa ccacaccact cccattcacg aaataccatc
    21661 attcatcgat ttgcctaaag gtctgtcaaa actgacttta aaaaaaataa gtgcatttat
    21721 tctaaaagaa gatactattt tttcaacaaa agaactggct caaaaaagct cactgtcacg
    21781 tatatccgtc aagaaatatt tgaatttttt agtagaacaa gatatattga acgaaagaat
    21841 caaatatttg gaaatcggcc ggccgctaac ctgttatgaa ttgacaaaga atgggcggaa
    21901 aaagctcaca gattactgtt gagtcatcga attatcccat acaaaatagt ccaagtatac
    21961 agaagtaaca actgtttact tggactattt ttaaacattt tattgacgaa tatccatgtt
    22021 ttcttatctt aattgacggg ctttaggaag agatacctca tttttcgttt ttccagtttc
    22081 tattatttca cgaaagttct cgaaacttgt cgaaatcata tttgataatg cctcatctgt
    22141 atttgaacct acatgcgggg ttacaagaac tctagggtac atttctacta attgctggat
    22201 agcagggtct ggaatcgtct cccatggctt gaaggatcgg aaaaaaacct ctgtttcatt
    22261 tgcaaaaaca tcggttgcaa aaccagctaa atgattgctt ttcaaagctc ttaaaatagc
    22321 ttcattatct tgaagctctc cacgggctgt attaatcaag atgctacctt ttttcatttg
    22381 agctaaaaat ccttcattga tcatttgatc gttttcacct gggatatgag gtacatgaat
    22441 actgacaata tcgcttttgg ctaataactc ttcaagcgag tggaacgtca atactttttt
    22501 agcagcttct gtctgatgaa tatcgtagcc gatcactttg gctcctagtc ccttaaacaa
    22561 aacagcttcc gtcaacccta tcctgcctgt tcctatgatc cctactgtgc aatttctgat
    22621 ttcacgacta aacatttgct catccactcg aaaatctttg aaagatgttc ggatcgtcgt
    22681 atacgcagta tttctcaaaa gcatcatggc aaaagtcaaa gataactctg ctatcgcatt
    22741 tggcgaatac gatggtacac gggctactgt cataccaaaa tcagctgccg cttgtagatc
    22801 gatatgattg actcccacag tccgtgtaaa aacataacga atcccatatt cctgcatttt
    22861 tgcaagattg gtctcatctg ctatacaatt tcccctcaac aatacagcat catggtcctt
    22921 tgctgacacg atattgtcat gtgtcaacag ttcttcataa agactgagat cataatggta
    22981 aatattcaat ttttcaaaaa aatctatctc gttcggacga acaccgtaac atgcgattct
    23041 catactttct tcctccattt cttatgaaaa taaacctatg ccatgaatca tttgtaatac
    23101 aacgatccat acaggtgtca gtaccacagc aaaaatagtt gacagtaagg atgcattgga
    23161 ggctaaaagt gcctccctat caaaactgat agcataggct gctgccactg ttgcagctgg
    23221 tgtagccatc atgataacga ttgttgccag tgcttcaaaa ttgactggta gaatattcgt
    23281 tgccgtcaaa actgctagaa tcacgatatt caatgcaggt acaaatacca ctttgactag
    23341 actgtaatac catgaagttt tttcagatgc agcttttttg aagcttactt cacctaatgt
    23401 tgcgccgatt gctagccacg ccaatggtga acttaaacca gctaaataat ccatcggctg
    23461 aaatagccac ggtgcagtct ggtcaattcg caaaaacgct acttggctta tctctccttc
    23521 tgcacctgta actgacactt gtggcagata cccttggaac agccaaatga aaaggccggc
    23581 aaaagtagcg ataatgattg gattgacgaa catcgctttg atatttttag cttccatctt
    23641 caagccactc atttttatgt atccataaga atacaaaaag attcggtagc caatattgaa
    23701 aattgaggaa tacatcacgc cgacaggacc ataaatcgca ctgacgatag gcgttccgaa
    23761 aaaggtcgta gaaccaaaga tcgtcaggac acgcaacgtt gtttgcttat cccctttata
    23821 gcttaagaaa aaaggttttg aaactagaat aagaataata taaataacta atccccaaat
    23881 caagacattg attccttgca tcagtaattc tgtattgata tcttgcatga aagctcgaaa
    23941 tgccagtgct ggcaaagcca ctgttaagac gactttcgat agaatttttc caacagattc
    24001 agaaaaaatc cccttttttc tacaaacaaa ccctaataat ataataaaaa ctgttgaagt
    24061 gattgcactg acaatcgaca tatctgtcaa agtcgatcga aaaatgtcaa ataagttcat
    24121 ctttctcctc ctaattatgt tcatttattc acaataaaaa aagcttttat tttgctaata
    24181 cgtattaaca tttttaattt tatctttaca ttgttactta ttgaacaata ggaaattaaa
    24241 gaaacttttg aaacttatgt aaagtgattt ttactagaac aca
//
LOCUS       DACDRN010000038        23077 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDRN010000038 DACDRN010000000
VERSION     DACDRN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.038"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(186..311)
                     /locus_tag="IWJ80_001741"
     CDS             complement(186..311)
                     /locus_tag="IWJ80_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9461457.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(445..1338)
                     /locus_tag="IWJ80_001742"
     CDS             complement(445..1338)
                     /locus_tag="IWJ80_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP9461458.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(1520..2446)
                     /locus_tag="IWJ80_001743"
     CDS             complement(1520..2446)
                     /locus_tag="IWJ80_001743"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP9461459.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(2531..3292)
                     /gene="pflA"
                     /locus_tag="IWJ80_001744"
     CDS             complement(2531..3292)
                     /gene="pflA"
                     /locus_tag="IWJ80_001744"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP9461460.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(3536..5767)
                     /gene="pflB"
                     /locus_tag="IWJ80_001745"
     CDS             complement(3536..5767)
                     /gene="pflB"
                     /locus_tag="IWJ80_001745"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP9461461.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6044..8494)
                     /gene="parC"
                     /locus_tag="IWJ80_001746"
     CDS             complement(6044..8494)
                     /gene="parC"
                     /locus_tag="IWJ80_001746"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP9461462.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(8506..10551)
                     /gene="parE"
                     /locus_tag="IWJ80_001747"
     CDS             complement(8506..10551)
                     /gene="parE"
                     /locus_tag="IWJ80_001747"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP9461463.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(10717..11151)
                     /locus_tag="IWJ80_001748"
     CDS             complement(10717..11151)
                     /locus_tag="IWJ80_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP9461464.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11278..11916
                     /gene="plsY"
                     /locus_tag="IWJ80_001749"
     CDS             11278..11916
                     /gene="plsY"
                     /locus_tag="IWJ80_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP9461465.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12048..12923)
                     /locus_tag="IWJ80_001750"
     CDS             complement(12048..12923)
                     /locus_tag="IWJ80_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP9461466.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13005..13796)
                     /gene="codY"
                     /locus_tag="IWJ80_001751"
     CDS             complement(13005..13796)
                     /gene="codY"
                     /locus_tag="IWJ80_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP9461467.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(13814..15214)
                     /gene="hslU"
                     /locus_tag="IWJ80_001752"
     CDS             complement(13814..15214)
                     /gene="hslU"
                     /locus_tag="IWJ80_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP9461468.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15228..15776)
                     /gene="hslV"
                     /locus_tag="IWJ80_001753"
     CDS             complement(15228..15776)
                     /gene="hslV"
                     /locus_tag="IWJ80_001753"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP9461469.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(15813..16718)
                     /gene="xerC"
                     /locus_tag="IWJ80_001754"
     CDS             complement(15813..16718)
                     /gene="xerC"
                     /locus_tag="IWJ80_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP9461470.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(16916..18292)
                     /gene="trmFO"
                     /locus_tag="IWJ80_001755"
     CDS             complement(16916..18292)
                     /gene="trmFO"
                     /locus_tag="IWJ80_001755"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP9461471.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18261..20339)
                     /gene="topA"
                     /locus_tag="IWJ80_001756"
     CDS             complement(18261..20339)
                     /gene="topA"
                     /locus_tag="IWJ80_001756"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP9461472.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHGHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(20461..21318)
                     /gene="dprA"
                     /locus_tag="IWJ80_001757"
     CDS             complement(20461..21318)
                     /gene="dprA"
                     /locus_tag="IWJ80_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP9461473.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21374..22141)
                     /locus_tag="IWJ80_001758"
     CDS             complement(21374..22141)
                     /locus_tag="IWJ80_001758"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP9461474.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22134..22994)
                     /gene="ylqF"
                     /locus_tag="IWJ80_001759"
     CDS             complement(22134..22994)
                     /gene="ylqF"
                     /locus_tag="IWJ80_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP9461475.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         6028 a         4997 c         3982 g         8070 t
ORIGIN      
        1 atttatgtgg caaagaaaaa acaaacaaaa taaaaagcat tttgaagcct gactattacg
       61 acttcataaa tgctttttta gttttaaaca cttcaatcta aaagaataac ccaagattgg
      121 ataaattatt aatgactcgc cattcgtttc ttagtgattc tggattatca aatggacttc
      181 ctaatctaat cagctagttc ttcccataaa atgccatact tgactgaatc ataatattga
      241 ttatttaaaa atctaacttt tcttatcact acttcctttg tcattccaca tttctcacca
      301 tttttttgca ttcctttgtt tccagaccat gtagtgaatc taataggtgg tagataggca
      361 aaatactcaa ataattcttt taaccataaa ggaagagaaa ggctcctcct gcaagagtca
      421 gtataatttt tgcttactgc tctcttaggt tttactcact aaaactgttc catattcttt
      481 gatataagaa aatgcatgcg gtataaattt cttgctcagc tcagaaaaga tttgtcgttt
      541 aagaaaggcc atttcctcca ttgtcaatga aggatccaat gttggtaatt caatctcaaa
      601 attgtttttt tcgacaatag gtaattctaa tgtgaatgat tcttcttcct ttatagtatt
      661 cgtcacaagt agagactgat ggaaaatact aggccggcga tttttatatt tattcttacg
      721 tatcctgtca acgataactt ctatttgatc aaaaaagtac gattcattta cgtctccaat
      781 aagttcggct aattctttga atgccttcgt tacaggaagc tgattcttct gtacaaaaaa
      841 gtaattagcc aaagtaagcg ggaagtgttc tatagaggaa agtgtgataa aacggtactt
      901 ctcatttcct gcagttgtcc tcagcaattc tgtctctcgg ctgtttctta ctccataaaa
      961 atacgcttct ttccgttcat cttcgaaaca atacgcccaa ataacttctc ccatcgctaa
     1021 tttttgttca tcaacagaga gtctcttcaa ttgttcagct atggtttcag cagccgaagt
     1081 ggcttgttgc aagtaatctt taggatcaaa tattggaaag ttcaaatgat atcttcgttc
     1141 ttccctcttg attaatcctg catcgatcaa taaatcaaga aaatgggaaa atttattttg
     1201 cgaaaattct tttttcaatt ctcttagaat cgtttcttgt tctcgatgtt catatagata
     1261 atcggtcaat gaagaaaaaa gcggatcatc ggtcagtttt tgataaaagt tttccttatt
     1321 ttttgaataa tagttcatat atgttctctt ttctatccgt tttattttac ttaaaaggct
     1381 tttacgtaaa aaggaggaag aacaagagtc ataattgact gctattctcc tccatttctt
     1441 cggcctttag tatgttctat gtttcttttt atatcaatcg cttatccatt cattttgaat
     1501 gcttcatact accatttgat tagctaaata cagccgtcaa ttgaggtacg acttgttttt
     1561 tacgtgaaac gacaccttta agcaaagcac ggttgttggt caaagttgtt tggaaagctt
     1621 cttctacttt ttcttttcct ttaccagcaa caagtaattc tgaatcactg tctaagatgt
     1681 ttgtgacaac taatacgaac agatcgtagc catttgcttt catttcatct tccattgctg
     1741 cttctagctc tgcttgtcga tccatcactt cattaaggtc cactgtattg acttgagcga
     1801 tacgaacgtt ggaactgccc attgggaaac tttttgcatc caaatcaaga agaaccgcag
     1861 ctgatttatc acttagattt gttcctgctt tcaacatttc aagtccataa gtgtttagat
     1921 cgacttcagc gattgctgca agttcttttg cagctgcttc atcttcaggc gtacatgttg
     1981 gtgatttgaa caacaatgta tctgaaatga tagctgagag catgattcct gcgatttctt
     2041 taggaattgt tacttggttt tctttatata gttttaaaat aatcgtactt gtacatccaa
     2101 ctggttctgc acgatagtac aatggattag ctgtttcaaa gtttgcaata cgatggtggt
     2161 ctactacagc taaaatattt acttcgctga tatctgaaat actttgttgg aattcattat
     2221 gatccacaag catgacatct gcattttctg ctttagctga ctctactact cgtggtgcag
     2281 atagctgaaa gtggtctaat gcgtattgtg tttcttcatt aggtgtacct agagcaactg
     2341 cttctgtttc tttacctaat tcattttgca agtgtgaaaa cgcaatggct gccccgattg
     2401 catctgtatc tggattttgg tgaccaaaaa ctaaaatttt tgacatgtca ttcgctcctt
     2461 tgtgatttct cctatttaat aatagcaaaa aaacggaaag aagcaatact tatcttcttt
     2521 ccgatgcgac ctatcgcgtt aaatattttg tatactcttc tgtatgaagc aatcgtttag
     2581 cattttcgac acgttctttt gtaggtggtt cgatcccttc taatggatat ttcaaaccaa
     2641 gtgtttccca cttgtatttt cccatcgtat gataaggaag aatctctact ctgtctacat
     2701 ttgacaaggt ttgaataaag ttgtctagtc ggatcaaata ttcatcataa tcacttcgct
     2761 caggaactag tacatggcga atccaaaccg gtttgttgat ttttgacaaa tattgagcca
     2821 tttctaaaat attttcattg tcccaatgcg ttagtttttt atgctcttca ttatcgatgt
     2881 gtttgatgtc aaataacagt aaatctgtat acttcatcaa ttcttgaaaa cgtgaaaaga
     2941 aaggttcttc atacgtaaaa ggttttccgc atgtatccaa tgtagtatgc atcttccgct
     3001 cttttgcttt tttaaatagt tcgatcaaaa aatcgatctg caatagaggt tctccaccac
     3061 taacagtaat accaccttta tctccccagt aaggtctgaa gcgttctgct ttatctaaaa
     3121 gttcatctgc tgtgtattct ttccctccgc caatattcca agtatctgga ttgtgacaaa
     3181 attggcaacg catgcggcat ccttgcataa acacgacaaa gcgcagtcca ggtccgtcta
     3241 ctgaaccgaa agtttcgata gaatgaacat agccgattgt tttttcttcc atttaagttc
     3301 acatcttttc tttgatttca ttttaagtac aagttagctg tcgaagcgaa gctttctgaa
     3361 caaaattttt cgcaattgcc gcttcgttat gtgtaaaatt tacagggacg gaacatacaa
     3421 gtcctgcccc tgtaaatcgt ttctttcaaa taagcatact gattattcga ttttgatgcg
     3481 atggctattc aacgtccttt cgacgctaag tcgaaaaact tttttcacga actttttaca
     3541 ttctgtcgtg tgaagttctc atcaagacat ctaattgttg ttctcttgtc aagtcacgga
     3601 atttaactgc ataaccagat acccggatcg tcaagttcgg atatttcatt ggattttcca
     3661 ttgcatcacg caataaatca ttactgaata cattgacatt caaatgataa gctccttttg
     3721 agaagtatcc atctaagaca ttacgcaagt tttcgattct tgtatcttgt tcttttccaa
     3781 gacctgaagg attgattgtt tgggtatttg aaattccatc caatgcacag ccgtaattca
     3841 ttttagctgt tgagttcagg ctagctaata acccactttg ttccgcattg taacttggat
     3901 ttgctcctgg cgcaagtggt tttccagctt cacggccatc aggagtattt cctgtatttt
     3961 taccgtaaac aacatttgaa gtaatcgtca ataatgaagt tgttggtttt gagttgcggt
     4021 aagttttatg acgtttgatt tgtcccatga agtattccaa gatccatgaa actaattcat
     4081 ctgcacggtc atcatcattt ccgtatttag ggaagtcgcc atccacttca aaatcaactg
     4141 ctattccttt ttcattacgg attgctttta cttttgcata tttgatcgct gacaagctgt
     4201 ctgccgcatg agagatccca gcaatacctg tagcaaatgt gcgttgcaag tcagaatcca
     4261 tcaaagccag ctgagctgct tcataagaat atttatcatg catataatga atgatgttca
     4321 acgtattgac atatagttct gctaaccaat ccatcatatt catgaagtta tgtttcactt
     4381 cttcaaagtc tagtatttct gatgtgattg gacgatattc tggtccaact tgggcgccag
     4441 tcacttcatc gactccgcca ttgatggcat ataatagtgt tttagccaag ttagcacgag
     4501 cgccaaagaa ttgcatgtct tttccgacaa ctgttcctga tacacagcaa gcaatcgcac
     4561 aatcatcaga accccattgt tcacgaagct gattatcatt ttcgaattga atcgaagaac
     4621 tttcgatagc gattctgcta gcatatgttc tgaatccttc tggaaggtgt tcagaataca
     4681 aaacagtcaa gtttggttct ggtgaaggtc ccatatttgt taatgtatga agcagacgga
     4741 aatcattctt tgtaactaat gaacgaccat ctaatcccat acctgcaatg gataaagtag
     4801 cccagatagg gaatcctgag aataattggt tatattcagg tgtacgcgcg aatttgacca
     4861 tacgtaattt cattactaag tggtcgatca attcttgtgc ttctttttct gtgattactc
     4921 cattatccaa atcacgttgg atatagatat caaggaatgc tgagatacgt ccgattgaca
     4981 tcgcagcacc attttgtgat ttgattgctg ctaaatatcc gaagtatagc cattggacag
     5041 cttcttttgc atttgcagct ggtttagaaa tatcatagcc atatttcgca gccatttctt
     5101 tcatttgcgc taatgcacga tattgttcag tgatttcttc acgtaaacga atcacttctt
     5161 cactcattgt atgataacca gtcatatcat gatctttttt cttttgttcc atcaaatagt
     5221 cgattccata caatgccaca cgacgatagt caccgatgat acgtccgcga ccgtaagcgt
     5281 ctggaagccc tgtaatgatt ttgtttttac gagcagcacg catttcagca gtataagcgt
     5341 caaatacacc ttgattatgt gttttgcgcc agtcagtgaa aatatgtgtc aattcatcgt
     5401 ttggtgtata gccattgctt tttagcgcat tgttagccat attgattccg ccgaacggca
     5461 tgaatgcttg tttcaaaggt ttgtctgtct gtagaccgac gattttttcc aaatctttat
     5521 ccaaatagcc tgcatcgtgt gaagtaatag ttgatacgat ttctgtgtcc atatcataga
     5581 caccgttacg ctcgaattgg atatcattta acttttgcaa ttcatcccat aatttttctg
     5641 ttgcttctgt tgggccttcc aaaaagctgt catcaccgcg atattcagtg tagttatttt
     5701 gaataaagtc tctagtgtct acgccttctt tccatttggt acctttaaat ccattccatt
     5761 gctccattgt atggacctcc taaatttctg ttttatgtaa cagtttttac gtgattagta
     5821 taacacagca ccctatcttt gcaagggttt tcttgttttt tatttaacaa tttttatgtt
     5881 ttatttacat atatttaaat caacgttgac ataccaacat ttaagcattg tgaacacttt
     5941 catctttttt tgcaaacgta taaggattta ttaaaaatga atgaaaaaga gacgccacct
     6001 gtattaatac agatggcgtc tcttcattaa aatactacaa cagttacgac tcgatgaaaa
     6061 tctcaggagc atcgataact tcgatgactt ctccaccttt tttctcatcc atcgcaaatg
     6121 atccattcga tgttcgatca cctataggga aatcagttgg gtcaatcgtt aactgctgcc
     6181 ctttttgatt catcaaggtt atttcttttg atcttccttc cgacatatag ataatacggt
     6241 gcggattttt tttcagctca cgcatgaccg tcactccccg cttcgcacgt gaagtttggc
     6301 tgatttcttg agcaagcatg cgtttaacag ctcctcgctg agtaacgatt acgatcggcg
     6361 tatcaccttc tgctaacaca agcagcccgt tgataatcct atctccatct ttcaaattca
     6421 tagacttcac tccagctgcc tttgctccaa caacgggaac atctgttaaa ggatagcgca
     6481 aacccattcc ttgatgcgtt actaagaaaa catctaagcc tggctgatct tctttcagat
     6541 acacagcaat cagttcatct tcttccgcct ttagcttcat acagctgagc ggacggctct
     6601 tataggtgcg ccatggttcg aactctgtca ttcgtgtttg cttgatcaat ccattcttgc
     6661 tgatgaatac aaatgttttc tctgcttgaa ttttttgata tgggaaaaca gctaaaatcg
     6721 attcgtctac agacagattg gagattgttt gagaaatgtg ctcaccagca tctttccatt
     6781 tcaaatcggg tagttcatgg acagggcggt agatcacatt tgctttattt gtgatcagaa
     6841 gaacatggtc caatgtactc aattcacctg agtatagcaa gtagtcgcct tctcgcatcc
     6901 cgatttcttc tggtttggaa gcactataag aacgaaggct tgttcgcttc acatagcctt
     6961 ctcgcgtcac actgacaatt acgtcttctt gtgccactaa gacttgcgta tcaattttga
     7021 tttcttcgat ttcgtcttcg attgtcgtta gacgagcgga agaataattt tttttcactt
     7081 ctcgcaattc ttttttcata acagagaaaa gctctttgtc atttgaaagg attttattca
     7141 actctgtgat ttgcgcaatc aagctttcgg attccttttg aagctctgtg atatccgtgt
     7201 tcgtcaaacg gtataattgc aatgtgacaa ttgcttctgc ttgttcttct gtaaattgaa
     7261 agacgtcgac taaattcttt ttggcgtctt ttttgtcttt actttcacga atcgttgcga
     7321 ttacttcatc cagaatcgat aaagctttca tcaatccttc tacgatatgc tgtctttttt
     7381 gagctttggc taaatcgaat tgactacgtt ttgttatgac ttgctttcga tgctcgatat
     7441 aacttcttaa aatgtctttc agtccaactt gatgcggagt catatggtcg atagcgacca
     7501 tattgaaatt gtaattgatc tgtagctcag tgtttttgaa caaataattc aaaattcctt
     7561 gagcattggc gtcttttttc aattcgacga caatttgcag acctgttcta tcactttcat
     7621 ctcgtacttc agctattcca tcgattttct tattcaaacg gatctcatcc atttttttca
     7681 ccaaggtcgc tttatttact tcatatggga tttctgtgat cacgatttgc tgtttattac
     7741 ccttcattgc ctctatcttt gttttcgaac gaagaataac ttttcctcgt cctgtttcat
     7801 aagctttttt gatttcatct tttccttgca agatccctcc tgttggaaag tcaggtccag
     7861 gaatatattc catcaatttt tcaaggctcg cttgtggatg atcgatcata tagattgtgc
     7921 catcgatcac ttcagctaag ttatgagtcg ggatttctgt tgcatatcca gcagatatac
     7981 ctgtcgatcc attgacgaga aggtttgggt atcgagctgg aaggactgtt ggttcttttt
     8041 ctgtatcatc aaaattccac acgagatcga ctgtttcttt ctcgatatct gctagcatct
     8101 ctccacttaa cttggataat ctggcttcag tataacgcat cgcagcaggt ggatcaccgt
     8161 ccatactccc gttattcccg tgcatttcga tcagtacttc ccgcaacttc cagtcttggc
     8221 tcatgcggac catcgcttca taaatactga tgtcaccatg aggatgataa ttccccatga
     8281 tatttccgac agattttgct gatttacgga atcctttgtc atacgtattg ccatctttgt
     8341 tcatggcaaa taatatccgg cgttgcactg gcttcaatcc atcacggata tcaggcagtg
     8401 cacgttcttg gataatatat ttggaataac gtccgaagcg gtcacccatc acttcttcta
     8461 aagttaattc ttgtacttct tgtcgatttt ccatcaggtg acctcctatt ctacttcaaa
     8521 taactgattg ttttcatttt tatctgctcg ttcttcatct aataaatcat tagaaactga
     8581 aggagagatt tcagtatcct cttttttatc taagatactg ccatcttctt cgagcgtaaa
     8641 ttgcacgtga ttttcgatcc attttcttcg aggttctact ttgtcaccca tcagtgtggt
     8701 tactcgacgt tcagcttgcg cagcatcatc gatacggacg cggatcaacg tacgggaagt
     8761 cggatccatc gttgtttccc acaattgttc cgcattcatc tctccaagtc ctttatatcg
     8821 ctgcagcata tatcctttgc cgactttttt aatcattgct gccaattcgt catctgtcca
     8881 cgcatattca atgacttgct tctttccttg tcctttggat actttatata aaggcggtaa
     8941 cgcgatatat actttcccag cttcaatcag cggtttcata taacgataaa agaaagtaag
     9001 cagtaatact tgaatatggg cgccgtctgt atccgcatcg gtcatgatga ttactttgtc
     9061 gtaattacag tcttcgatag aaaactcagg accgactcct gcaccaatcg tatagatcat
     9121 cgtattgatt tcttcatttt tcaagatatc ttgcatttta gctttttcgg tattgatgac
     9181 ttttcctcgt aaaggtagaa ttgcttggaa ctttctgtct cttccttgtt tagcagatcc
     9241 gccagcagaa tccccttcga ctaaatacaa ttcatttttc ttagggtttc gggattgtgc
     9301 gggtgtcaat tttcctgaaa gaagagattc gcctttttta cgcttcttcc catttcggct
     9361 ttcttctctg gctttacgtg ctgcttctct cgcttctctg gctttgattg ctttacggac
     9421 caacatctgg ctcatttcgc tattttcctg caaataaaag cccatttgtt cactgatgac
     9481 gttatctact acggtacgag caactggcgt accaagtttt tcttttgttt gcccttcaaa
     9541 ttgcagaaga ttttcaggta cacggataga aagtacagca gctaaccctt ctctgaaatc
     9601 actaccttcc agattcttgt cccgttcttt cagcaaacca acttttctcg catattcatt
     9661 ataagatttc gtcatcgccg ctttcatacc agcttcatgg gttcccccat cttttgtccg
     9721 aacgttattg acaaaagata aaacattttc tgaataaccg tcattgtatt gataagcgac
     9781 ctctacttcg ataccttctt tttctcctga aaaataaaca actggtgtca atgtatcttt
     9841 ttcttcattc agataatcaa caaattcttt gattccttct tcgtaatgaa aaatttcctt
     9901 gactggttct tctccacgaa gatcagaaag agagatttta acgcctttta gaagaaaggc
     9961 agattctctt aaccgttcgg ctaaaatttc atatgaaaat ttcgttgtac tgaatattgt
    10021 atcatctggt aaaaaatgga cagtcgtccc gtttgctttt tttgtttttc ctatcttttt
    10081 caaagtaccg tcaggtttac cgccgttttt aaatttctgc tgatactcta cgccatctct
    10141 aacgatcgtt acaattagcc atttcgataa ggcatttacc acactggctc ctacaccgtg
    10201 cagtccacca gaagttttgt accctccttg accaaacttt ccgccagcat gcagcacagt
    10261 aaaaatcact tcaactgttg gtattccgga agcatgcatt cctacaggca tcccacgtcc
    10321 gctatctgca acagttatac tattgtcttc atggatggtc acatcaatct cactgccata
    10381 acctgataat gcttcgtcaa ctgcattatc gacgatttca tagactaaat gatgcaagcc
    10441 tcggctgtca gtggatccaa tgtacatccc tggtcttttt ctaactgcct ctaatccttc
    10501 caatacttgg atggacgagt cattgtattc gttgtttact ttttttgcca aggcaaacgc
    10561 tccttcaata atactttgcg gcaaaaccgc accacgatta tcatactaga aaaggtcgtg
    10621 aaaaaaaaga gggtagacga gaaatcaatt ttaattatct cctaaccgct ttttcgacat
    10681 tcccctctat cgatcatctc ttctttgtgg tcatttttat ttttggtttt ctttcaactt
    10741 accaagttca atcttaatac aacgatccat tacgatatct gtacgtcctg cttttcttag
    10801 catctcagct gcttctttgt tttcaatccc taattgtgcc cagaacactt tagcatcagt
    10861 tttcaaaaaa tcacctgcta tttcaggtaa aaattcactc cgtctaaata catctacgat
    10921 atcgattgga tcatcgattt ctgtcaaatt cgcatagact ttctcgccta gaatttcttc
    10981 accagccaat aatggattta ctggtatgat tttatatcca ttgtcttgca atacctgtgc
    11041 aaccatgtaa cttgttcgtt ctggtttatt gctcaagcct acgacggcaa tattttttgc
    11101 ttcttccaaa tagttgaaaa tcgtttcttg tgttggattt tttacagaca tttctgccac
    11161 cccttgcttt tctttttatt ccatctatta tacttgtatc tttcattttt tcaattgttc
    11221 ctgttaccta ttcatgctaa aataaaggat agttttcaac ttgaaagagg gtaaaaaatg
    11281 aaattgatct tattacttat tttagcatat cttttaggtt ctattccttc cggtgtctgg
    11341 attggcaaat tgtttttcaa aaaagatatc cgacaacacg ggagcggtaa tactggaacg
    11401 accaatacat ttcgtgtatt aggcaaaaca gccgggattg ttgtcctgct tatggatatt
    11461 ttaaaaggta cattggctac ttgtttgccg atgatcttcc acctttctgg gattaaccct
    11521 ctttggtttg gggtatgcgc cattcttggc catacatttc cgatttttgc tgggttcaaa
    11581 ggaggaaaag ctgttgcgac aagtgcagga atgcttttag gatttaatcc tgtatttttt
    11641 gtttattctt gtatcatctt tatcgttagt ttattttgta caagtatggt gagtttgagc
    11701 agtatgatca gtgcagtatt gatcacttta tctacgatcg tccttcctta tgtagctcct
    11761 gtgattttgg ctaagcccaa ttggttactg acgatcattg cctttttagt ttcttccttt
    11821 atcttttatc ggcataaaga caatatcaag cgtatcagaa accatactga aagtcgaatc
    11881 ccgtttggtt tgaatagcag caagaaaaaa tcctgatact ttttcattcc aaagagataa
    11941 aacgggagtg tgacaaatcg atgtcacact cccttattac ttacaggata gcttttttac
    12001 acaccttggt aaacggtgtt ccctgctaac ttctcggtca caacctctta cttcactgtg
    12061 atggaatatt ttgtcttgaa gatttcatgg gcacctagtt gattgatccc tactttttcc
    12121 atcaagtttc cactactgtt caccgtgtca gcgattccgc accacggttc gatacaaacg
    12181 aaaggcgctt cttttggata aggagaccag atccctacat aaggcatatc tttatagctt
    12241 aaagttacgc tatgggaaga tttttcactt cgaatggcgt acgcatttag accccttgtt
    12301 tcaaaaacca aggcatcttg ctcaaaaagt tcacgattca aagccagatt cgtatttgtc
    12361 tgacctaacg tcttttgatc catatcgatg aatgggcctt tcaatggtag gcgtaatctt
    12421 gatttcaatg gagaaaatgc cagatagaaa tcttcaaatg acaatccttc ttcaagaggt
    12481 acattgaaag caggatgtcc accgatagaa aagaacattt ctttttcacc ggtatttgcc
    12541 acttgataat gaaccgtgat gccgtctcct cctaattcat agctgatcac taattcaaaa
    12601 tcaaatggat acttttctaa tgtctccggc gtactcctta agatgaaact agccatttct
    12661 tgtccatgtt caatgacttc aaaatccata tcccgagcaa atccgtgttg ccccatttga
    12721 tatgttttcc cttgataaac atactgatca tcttttaaac gtccaacaaa agggaataaa
    12781 accggtgcat gtctgcccca atacgctgga tcaccttgcc agatatactc taaaccatgt
    12841 tcttttgatt tcagactgat caattccgca ccgtgttcag cgatttcggc gatcagctgg
    12901 tcattttgaa taataacagt cattgccatt cctccaactt cgacttactt tttaaaatgg
    12961 gaaggctgga acaaacattt ctatctgccc cagtcccctt acttctaata actgtgtttt
    13021 gctaactcgt ctttaaaacg ggaattcaat acttttaaat aagttccctt catgcctaac
    13081 gaacgagatt caatgattcc agctgattcc aattttcgta aagcattgac gatcacagaa
    13141 cgagtgatgc cgatactatc ggcaatatta gaagcagtca gacgaccttc atctccatcc
    13201 aatgcttcaa agatagcttg tacagccttc aattcgctat aagataatgt gttgattgcc
    13261 atttggacag ccgttgcact ccgtacatct gcttcgatgc ttcgagactt ctgataaagg
    13321 atctgcatac cgacaaccgt agctccatat tctgctaaga ctagatcgtc atctgcaaac
    13381 gattgttcca tacgagataa gataatcgta cccaaacgtt cgccagctcc aaagatcgga
    13441 acgacagtcg tcagaccaaa tggatatttc tctcttgact ctaccggaaa tgcagtaata
    13501 tcactagtaa ttgggatatt agcttcagtt ttcatcaact gtgccactgt atcagtataa
    13561 gattcaggaa attgtttctc aacaaacatt gttttgattc gttcattgtt cacatcgtga
    13621 cgttcattat aacctagtaa tactccgtca cttcctatga tataggtgtt gctgtcaaga
    13681 ataccaccta gtgtgacagc cattttatcg taaggcaaat ctgcttgcat gtcaaaagta
    13741 ttcttttgct gcaaaagtgt gtttatcttg cgggttttcg ttagcaagtc tgtcattgtc
    13801 gtcctccgat tttttataaa atatagcgac ttaaatcttc atttgccgca attgagccaa
    13861 gtttgtcatc cacatatgct tctgtgatcg ttatttctcc catttgcata tctggtgctt
    13921 caaataatag atcttctagt agtttttcca aaatggtatg cagtctgcgt gcgccgatat
    13981 tatctgtgtc acggttgaca tcgaaagcga tttgagcgat ccgttcaatt gcttcttttg
    14041 taaacgtaac tgacacattt tctgtaccga tcaacgcgac gtattgtttt actaaagcat
    14101 tatttggttc ggtcagaatc ttcacaaaat cttcagctgt caaatcatct aattctaccc
    14161 gtatcgggaa gcgtccttga agttctggaa ttagatcact tggtttagac aaatggaaag
    14221 ctcctgaagc aatgaatagg acgtgatctg tttggatgat cccgtatttt gtgtttactt
    14281 gtgaaccttc gacaattggc aatatgtctc tttggacgcc ttctctggat acttctccag
    14341 attgctgtga tttagaagtg atcttatcga tttcatcgat aaaaataatt ccactttctt
    14401 ccgctaattt gattgcctca ctatggatat cagcatcctt gactaatttt gctgattctt
    14461 ccttgatcaa taactctaaa gcttctttga ctgttaccgt ccgttccact tttttcttcg
    14521 gtgtcaaagc acctagagtt tcatttaggt cgatccccat ttgctccaat ccattgttca
    14581 tcattggtgc aggtttcttt tggtcttcaa cttcaatggt tacttcacga ctatccagta
    14641 aacccttttg tagttgttcc aagactgtac ggcgattgat tcgaatatct tctgtcactt
    14701 cttcttgcgg ttcagcggta ttttgctgac tagcattaaa aagattcatc atttgttcaa
    14761 attgattagt ggattgtttc tgttcttttt tgataccagg taccaagatt tttaccaaac
    14821 gtttttctgc atttttttta gcacttgtgt atacacggct gtactgctct ttttctacga
    14881 tttggatcgc attttctact aaatcccgaa ccattgattc tacatctctt ccgacataac
    14941 cgacttcagt aaattttgtt gcttcgactt tgacaaatgg tgcattgacg atttttgcca
    15001 aacgtcgtgc gatttctgtt ttaccgaccc ctgtaggtcc gatcatcagc atattttttg
    15061 gtgtaatatc ttgttgcatt ttttcatcta actgcaaacg gcgatagcgg ttacgcaaag
    15121 caacggcaac agattttttt gctgcgtgct gtcctacgat atattcatct aattcactta
    15181 cgatttgtct tggtgtttgt tttaattcat acatatctac tcacctctta cagttcttct
    15241 acgataatat tatgattagt aaagatacag atatctgcgg caatatttaa cgcattttct
    15301 gcaatttctt tcgcactcat gtcgttttga gcatagtttt tcattgctcg ggctgcagcc
    15361 aacgcaaaat tccctcctga tccgatagct aagataccat catctggtgt gataacttct
    15421 cctgtacctg aaacaagaag catttcttta tcattcatta cgatcagcat cgcttcaagt
    15481 ttttgcatag attgctgtgt ccgccattct tgagctaatt cgacagctgc gcgttgtaga
    15541 ttcccgttat attcgtttag tttgccttca aatttttctt ctaaagtaaa cgcatctgca
    15601 acacttccgg cgaaacctac aacaacttct ccattgtaga tacgtcgtac tttctttgca
    15661 gtgcctttca tgacaacaga ttcacccatt gttacttggc catctccagc catagcaaat
    15721 ttcccgtctt tttcgacggc acagatagtg gttgaatgaa atggtgattc gaccatgttt
    15781 gtttcctcct aaatttgttc ttttccttat ttttacgctc ttggatggaa agaacgatag
    15841 tttttttgta aactttcttt tgttacatgg gcatagatct gggtagtcga taaattggca
    15901 tgtcctagca attcttggac tgtccgcaaa tctgccccat tatttaataa atgagtagca
    15961 aatgtatggc gcagcatgtg aggatggata tcactatcca aactgctttt tttgatcatt
    16021 tgattcaaga catattcaat tccagtaggc gtgattggtt ttccatgatg attcacaaaa
    16081 acgatatcat gctcttcgtg atattttgtc atcaatacct ggcggccttc cctgaaatat
    16141 tcttgcagag catcttgagc aaaagaacct agaggagcat atctctcttt gttcccttta
    16201 ccatggatca acatgacctc tgaatcaaaa tcaatgtctg ataccgacaa actgctacac
    16261 tcacttaaac ggatgcctga cccgtaaagg acttccagta aagcgcggtt cctcaaatcc
    16321 aaaggactat ctcctgctac gctgtcaaaa agtgcttgca tttcattttc ataaaaaaaa
    16381 cgtggcagtc tcaaattttt ctttttgaga tgaacgtaag aaaatggatt ttcttttatc
    16441 acttcttctt tgagaagata ctgataaaac gaacgtaggc tggctatctt gcgactgata
    16501 gaatttctgc tatagctagg acgatgatca gctaatgcac tcaaatagac acgaaccgta
    16561 aaatattccg ccttcagcag atcagcgtct ccagattcct ccaaaaaatg tttgaaatgg
    16621 agaatgtctt cttcatatgc ttctttcgtt ttttctgaat atcctctttc gacaatcaaa
    16681 taacgcaaaa atttttgttc ccacgtggct tctgtcattt tctcaccttc cttttgtccc
    16741 agagaataat ttagcacagc gtttataaaa agacaaataa attgtcagaa tttaaccaat
    16801 atttctaagt ttgttcacaa ttggcgcaat tcctcacatt ttgtttgaga aattcttaag
    16861 aaaaacgcaa gaaaaagagc gagactatcc gtctcaccct cttcatggag tttccttact
    16921 catttgggat tgcagttttt tctaattctg tttttattgc ctcattagct tttaaagctc
    16981 gttgtgcaat tgcttcatat cttgcttttt tgtctcgaat gcgctctggc agttcaggaa
    17041 acagaccata gttggcattc atcggttgga aatgttttcc ttccgcatga gtaatgtagt
    17101 aagccatact tccaatagct gtttcttgtg gaaactcgac tggttccatt tcttttgcta
    17161 agcgtgcggc gttgattcca gcaagtagtc cgctcgctgc actttcaaca tagccttcta
    17221 caccagtcat ctgacctgca aagaacagat cttcgcgttt cgttgattgg taagtcggtt
    17281 tcagcaattc tggcgaattc ataaagctgt ttcggtgcat cacaccatag cgaacgaatt
    17341 cagcattttc taacccagga atcatccgga aaacacgttt ttgctcgccc catttcaagt
    17401 gagtctgaaa gcccacgatg ttgtataaag aagctgcagc attgtcttga cgcagctgga
    17461 taacagcata aggacgtttg ccagttttag gatcttccaa tccaactggt ttcataggtc
    17521 cgaaaagcat cgttttgaat cctcgctgtg ccattacttc aattggcata catccttcaa
    17581 aatatttttc tttctcaaaa gatttaagtt ctgccacttc ggcattgatt agtgcctcat
    17641 ggaaagcttt gaattcctct tctgtcatcg gacagttcaa gtaagctgct tctcctttgt
    17701 catagcggga cttcaaatat actttatcca tatcgatcgt agctttgtct acgattggtg
    17761 cagctgcatc atagaaataa aaaccatctg aaccattgaa tgctttgatg ctttctgcta
    17821 atggatgaga agtcaaaggt cctgttgcta ctacggtgat tccttccggt atttcagtaa
    17881 tttcttcgtt tatcactgtt actaatggat gctctttgac ttttcgggta attgtttcag
    17941 aaaaagaatc gcggtcaaca gctaaggcac caccagctgg cactgctgtt tcatctgccg
    18001 aagtgattac taccgaattc aatcggcgca tttcttcttt caataaccct actgcattag
    18061 ccaaactgtt tcctcttaat gaattggaac atactagttc agcaaaatta cttgtgtgat
    18121 gcgcctcagt cgatttgact ggacgcattt catataaacg gacagggact cctgcttgtg
    18181 cgatctgcca agcagcttca cttccggcaa gccctgctcc aacaacattt accattttca
    18241 atgaaagaac ctcttttcta ttatttttgg atgtcttctt cgtaatctcc atttacacag
    18301 actacttgtt taccgccttt gactttcttc tcgaccagat atccgtcaca ttttggacaa
    18361 tttctgccca ctggtttatc ccaagaagtg aattcacatt ctgggaaacg ggaacaacca
    18421 tagaatagtc tgtttttctt agattttcgt tcaatgactt gtccttcatg acatactggg
    18481 caagtcacac cgatttcttt tacgattggt ttcgtattcc gacaatcagg aaaattactg
    18541 caagcataaa acttcccata tttccctaat tttatcacca tcggatgtcc acagacatca
    18601 caatcgaatc ctgctggttc atctttgatt tgaatttttt cgattttttc ttccgcattc
    18661 gtcagttctt tttcaaacgg tttatagaaa cgatcaacca ctttgaccca cgcttccgtg
    18721 ccttcttcaa ttttatccaa atctccttcc atttctgctg tgaaatggat atcgacgatt
    18781 tgagggaaga attcacagat taatgaattg acaatttctc ctagttcagt cggctcaaat
    18841 cttttttgtg tcaattttac atagtagcgt ctttggatcg tatcaagtgt tggtgcataa
    18901 gtggaaggtc gccctacccc gttttcttct agtgcacgaa tcaacgtagc ctcactaaaa
    18961 cgtgctggtg gttgagtaaa atgttgtttt ggttcaatat caacagattg gactacatcg
    19021 ccttcttcaa gttctggaag aatattttct ttctcttctt tgccgtcatc tctaccttcc
    19081 acatacactt gcatgaatcc tttgaatttg atttttgaac cgttcgcaat gaagatgaca
    19141 ccattttgtt ctaatgtcac tttcattgta tctaaaatag ctggtgtcat ctgactagcc
    19201 acaaaacgtg accagatcaa tgtataaagt ttcaattgat ctttatctaa atattgtttg
    19261 agttcatctg gtgtacgaag cacactcgtt ggacgcacag cttcatgggc atcttgtgcg
    19321 ccttgagcat ttttgatctt tcggtgacca tgtctagaaa attcttttcc atacgtatct
    19381 tcaataaatg ctgcggcctc tgcttttgct gtatcggcaa tccgggtaga atcagtacgc
    19441 atgtatgtaa tcaagccgac tgttccttgt ttacccaaag tgattccttc atataattgc
    19501 tgtgctacca tcatggtttt tcttgttcga aagtttaact tacgggcagc ttcttgttgc
    19561 aggctacttg tagtgaaagg attcgcaggg tttcttttgc gctctttctt ttctactttt
    19621 ttaacttcga agtctgttcc atcaattcta gcagtgactt cttttacact ttgtgcatct
    19681 ggtaatttct ttttcttacc gtcgattccc cagaaatttg ctttgaattt cttgcgagct
    19741 tttttaaaat taccgtcgat gctccaatat tcttctggaa caaattctcg gatctctttt
    19801 tcacggtcaa taataatttt taaagcgaca gattggacac gtcctgcact taaccctttc
    19861 ttgaccttac gccagagaat aggactgatc gaataaccaa cgattctgtc taaggtacga
    19921 cgagcttgct gtgcatctac taaatctaca tcgatcgttc ttggttcctt aaaagctgct
    19981 ttgactgcgt cttttgtgat ttcattgaag accacccgat ttttttcttt cagatccaac
    20041 ccaagtaggt acgctaaatg ccaagcaatc gcttctcctt ctctatccgg gtcacttgcc
    20101 aagtaaactt tttgagcttt ttttgctgcg gctttcaggc ttttgatgac atcgcctttt
    20161 ccgcgtatag aaatataatg gggttcataa ttgttttcaa aatcgatacc cattttactt
    20221 ttcggtaaat ctcttatatg gccaacactg gccattactt tatagtttcg tccaagatat
    20281 ttctcaattg tcttggcttt tgcaggtgat tctacaatca ctaaatattt atacgccatt
    20341 actgaatgga ctcctttcca ttgtcgtgtt cctactatta aaagaggatt catacaaatc
    20401 gtagctaatc atatccattc agaaaacgtt tgtcaagaaa agcaaatgaa aagtatggtg
    20461 ttattcttta aattctgcca agatatcttc acccgaaacc gtgcaaactg caccgtcttg
    20521 gatcaaatga tgacatccgg tagaatgttc tgttaaaata tttcctggaa cagaaaaaac
    20581 tgttcttcca agatccaatg cttgctgtgc tgttatcaga gagccgcttt ttttcttggc
    20641 ttcaaagaca catgttcctt tagacaaagc tgcgataatc cgatttctca ttggaaaaca
    20701 atgctttttc gggccggatt gaggaggata ttcactcaaa agaagatgat catttgcaat
    20761 cacctcttgc aagtcacgat tttcagcagg atagacttgc tctatccctg ttccaatgac
    20821 tgcgattgtc cgtccaccat gagagattgc tgcttggtga ccgtatgtat ctatcccttt
    20881 cgcgcagcca ctgacgatca catatccagc ttctatcact ggttttagta aataataaac
    20941 tgtttttttg gctaaagcag aagtgtgtct tccaccaaca acgcttaaca tgttacaaga
    21001 aagtaaagat agatcaccat tataaaacag tacaattggc ggataggtca agtgaagcag
    21061 ctgaggggga tatttgggat caaagcaagt gataaacgac tgtttcgatt ggattttgcc
    21121 ccagttagcg tgaatttcct gccaactttt gcgaaacagc tcccggtgtt tggttatttt
    21181 acctatctta atgatgtctt caaatccgat ttctttataa ggatactgca tcatccagtt
    21241 agcaagtgcc cattttcctt gactactgat tccttttgta taagcaagtt tcaataaatt
    21301 ttcttctatt tgatacatca taaaaccgcc tttcgtctct ctttctaata agatacgtaa
    21361 aagcggctgt tttttattgt aaatattttt ttacaggtgc aaaactttta cgatggattg
    21421 gcgtaattcc ttgtcgttca agtccttcta agtgttcctt tgttccataa cctgcattat
    21481 gcgagaaacc atatccagga taaagcacgt catattcttt catcaattgg tctcgatata
    21541 ctttagcaat gatacttgca gcagcaatcg acacagatcg tgcatcccct ttgattattt
    21601 tttcctgctg aataggcaaa tccaaggtca tcgcatcgat caacaaatag cttggtacaa
    21661 tggacaattt ttctactgct tggatcatcg ctaatttact tgcttcataa atattcacat
    21721 catcgattgt ctgctgatca acgatcccga tcccgattgc cagggctttt tcttgaactt
    21781 gttgataaag aagttctcgc tttttctctg ataattgttt ggagtcattc agccctaata
    21841 tgatctcatt tttcggcaag ataactgcag ctgccacgac aggtccagct aatggtcctc
    21901 ttcctacttc atcaatgccg gcaatccatt ggtgaccttg tgcgtaccct tgcttttcaa
    21961 agcaaaacat ttcttcatat ttttccttaa ttaatgcttc ctttttcaat cgtcgttcaa
    22021 aagaagccag tgcttgctgc acgcctttgc gtggatcatt tcttaatgat tctagtgtag
    22081 gatcatctat tctctgtatg cttttcaatc gttctttgat actttggatg gattcagtca
    22141 tcactttttt gctccatttc ttcgcaacga tccagtgtgt atcgtccaag ttttccttga
    22201 cgaatatcat gcacgactaa ttcgctgcca cgatcatagt cttctttata cccacgtttt
    22261 gctgtaatat ccatcagtaa ttccggtgcc aatttttctt cttgctcgat tgataaacca
    22321 taacgatcgc tgattcttcc tgaataatat cgggcaaaaa agtctaatcc gtaaatcgct
    22381 aaatcatcta aatggacaag ctggtctttg atcgcgcctg tcagcgctag ttttttgccg
    22441 atttcttcat cttcaaattt gggccataaa atccctggtg tgtctaacaa ttccaactca
    22501 ttacctaaac gaagccactg ctgacctttt gttacgccag gcttatttcc tgtctgtgca
    22561 attttcttgc ctactagtcg attcatcaac gtagatttcc caacatttgg aatacctaag
    22621 accattgccc gaatagcgcg aggcttgatc ccccgagcag catctcttgc ccgtttttct
    22681 ttcaatgctt ctttagccgc aggtactaga cctttaattc ctttactttc ttgtgccgtg
    22741 atagctaatg caagatggcc ttgtttacga aaatattctt gccacagctt tgtctgttgt
    22801 ggatctgcca gatccgcttt attcaataaa acaaggcgag gtttttgctg aatgatctga
    22861 tctagcattg gattacgaga agataaaggt aatcgtgcgt ctaccaattc aaatacgatg
    22921 tccacatatt tcattttttc agaaacttct cgtcgggctt ttgccatatg tcccggaaac
    22981 cattggatgg tcattgttct ctccattcct ttcataccgg aatattcccc ggctttactt
    23041 gtttttttct ttttcaatat aaattcactt ttttatt
//
LOCUS       DACDRN010000039        22938 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDRN010000039 DACDRN010000000
VERSION     DACDRN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.039"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..832)
                     /locus_tag="IWJ80_001760"
     CDS             complement(<1..832)
                     /locus_tag="IWJ80_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9461476.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     Y"
     gene            964..1617
                     /locus_tag="IWJ80_001761"
     CDS             964..1617
                     /locus_tag="IWJ80_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9461477.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(1680..2207)
                     /locus_tag="IWJ80_001762"
     CDS             complement(1680..2207)
                     /locus_tag="IWJ80_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9461478.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(2227..3174)
                     /locus_tag="IWJ80_001763"
     CDS             complement(2227..3174)
                     /locus_tag="IWJ80_001763"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9461479.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(3186..5072)
                     /locus_tag="IWJ80_001764"
     CDS             complement(3186..5072)
                     /locus_tag="IWJ80_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9461480.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(5232..7310)
                     /locus_tag="IWJ80_001765"
     CDS             complement(5232..7310)
                     /locus_tag="IWJ80_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9461481.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(7323..7598)
                     /locus_tag="IWJ80_001766"
     CDS             complement(7323..7598)
                     /locus_tag="IWJ80_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461482.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(7960..8415)
                     /locus_tag="IWJ80_001767"
     CDS             complement(7960..8415)
                     /locus_tag="IWJ80_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461483.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(8569..9777)
                     /locus_tag="IWJ80_001768"
     CDS             complement(8569..9777)
                     /locus_tag="IWJ80_001768"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP9461484.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(9889..10221)
                     /locus_tag="IWJ80_001769"
     CDS             complement(9889..10221)
                     /locus_tag="IWJ80_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9461485.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            10369..11244
                     /locus_tag="IWJ80_001770"
     CDS             10369..11244
                     /locus_tag="IWJ80_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9461486.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(11326..11907)
                     /locus_tag="IWJ80_001771"
     CDS             complement(11326..11907)
                     /locus_tag="IWJ80_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP9461487.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(11913..13172)
                     /locus_tag="IWJ80_001772"
     CDS             complement(11913..13172)
                     /locus_tag="IWJ80_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9461488.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(13174..14220)
                     /locus_tag="IWJ80_001773"
     CDS             complement(13174..14220)
                     /locus_tag="IWJ80_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461489.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFMWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(14455..14730)
                     /locus_tag="IWJ80_001774"
     CDS             complement(14455..14730)
                     /locus_tag="IWJ80_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP9461490.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(14973..16283)
                     /locus_tag="IWJ80_001775"
     CDS             complement(14973..16283)
                     /locus_tag="IWJ80_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP9461491.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(16465..17694)
                     /gene="rpsA"
                     /locus_tag="IWJ80_001776"
     CDS             complement(16465..17694)
                     /gene="rpsA"
                     /locus_tag="IWJ80_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP9461492.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(17829..18509)
                     /locus_tag="IWJ80_001777"
     CDS             complement(17829..18509)
                     /locus_tag="IWJ80_001777"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP9461493.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(18558..19193)
                     /locus_tag="IWJ80_001778"
     CDS             complement(18558..19193)
                     /locus_tag="IWJ80_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9461494.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(19259..20662)
                     /locus_tag="IWJ80_001779"
     CDS             complement(19259..20662)
                     /locus_tag="IWJ80_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP9461495.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(20649..21680)
                     /locus_tag="IWJ80_001780"
     CDS             complement(20649..21680)
                     /locus_tag="IWJ80_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461496.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            21711..21932
                     /locus_tag="IWJ80_001781"
     CDS             21711..21932
                     /locus_tag="IWJ80_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP9461497.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(22097..22834)
                     /locus_tag="IWJ80_001782"
     CDS             complement(22097..22834)
                     /locus_tag="IWJ80_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP9461498.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
BASE COUNT         6375 a         4863 c         3883 g         7817 t
ORIGIN      
        1 aatacatgat tgcaaacgcg tctttaccag tatgcgtagc gataaccgga ttcgtatgca
       61 gtacagggat atgcatgtcc gggaataatt cttgcaaacc ctctttgaat tcttgcgcca
      121 attcttttgc atccgcatgt gaaataccga tttgtcgcac atttggcaat tctttcagtt
      181 catttttcaa attattaaac catttatcga acgttttctt tcctcgcccc ttagttacag
      241 ggattaattc agaatgggcg aaattcatca ccacgcgtat gttcaaaaca ctagacagca
      301 aaccagttgt tcggctgatt cttccacctt ttaccaagtt atctagtgtg gatattccga
      361 tgtataattt tgtattgtct tgtacttttt tgacttcttc aaggattttc tctactgttt
      421 taccttcttt tgctaattca gctgctcgaa ttacctgaaa agataatcct tggtcagtgg
      481 tatcactgtc gataaccgtt actttcgttt ttgttagatt actggcttgt cttgctgctt
      541 ctactgttcc actcaagcct tttgtcatat ggatcgaaat cacttcactg ccgtcttctc
      601 ctagctcatc atacatcgag atgaattctc caattggtgg ttggcttgtt ttgggcagat
      661 tttttgcttt cgccatcatt tccataaatc tttcgccgtc tagttggtca tcatcgggat
      721 atactgttcc gtcgatcatg atggatagcg gcatgacacg aatgcctaaa gcatcgcgta
      781 catttttttc catcgtacat gaagaatctg ttacgatttt tacatttgtc attattttct
      841 ctctctcttt ctttaacaag atgccttatt catgttaaac tacatgtaag acaatttcta
      901 tttttagtat aacagacaaa taatgatttt tcattaatta cacctagtaa aggagccgtt
      961 ttcatggaaa aaacgaaatt taatcgaacg tatgaaatat taaatgaagt gtttaatgca
     1021 gtcacccatg gaattgggac tggattgagt atcgcgggac tagtggttct tttagtaaaa
     1081 ggtgcccact tgcattcctc cctccatatt gtctcttatg ctatctatgg atcgatgatg
     1141 attcttttat ttttaacgtc tactttgttt cattcattga tttttacaaa agcaaaaaag
     1201 gtgttccaag tattcgatca tagttcgatt tttcttttga ttgcaggaag ttatactcct
     1261 ttttgtttgc tgagtatccg aggatggcta ggttggactt tatttgtatt aatctggttg
     1321 tttgcgattc ttggcattat atataaagcg ttgacgttgc ataaacggga gagagtatca
     1381 aaagtatcta cgatcatcta tatttttatg gggtggctct gtgtcgtggc tgctgttcct
     1441 ctttatcact ctcttggaat gactggtgtt attttgatgg tagcaggcgg gctttcttac
     1501 acgataggcg cattctttta tagcttgaat tctatccgct atatgcatgt tgtttggcat
     1561 ttgtttgtta tgttaggtgc aggttttatg tacttctctg ttttattctt tacttaataa
     1621 aaaaaataaa gctatggatg ggacagctct aaataacagc tagtctatcc atagcttttt
     1681 tatatcgtca attcgcctgt cccgcttttt acaggattct cattacgctg atacgtttca
     1741 aattgatgcg gataacgatt tttttcatcc atttctcctt gactagtatt gatcagtgac
     1801 caatcagacc agtcaacagg tggaaaataa gcatctccat cgaatacatg atggatgact
     1861 gtacggtata atatcgtaca aaatggcaga aattcttgat aaacagctga ccctccagcg
     1921 atgaaaaaga taccgtctgt ttctttttcc agagcaagca cgtcttccac actatgcatg
     1981 acatgcacgc cttctgcttt ataggaagaa tctctcgtca aaacaatcgt ctgacgattc
     2041 ggcaacggac ggctacccat cccttcgaat gtttttcgtc ccatcactag tgtatttgct
     2101 tctgtcattt gtttgaagaa tttcagatca tttggcaaac gccaaggaag acggtcttct
     2161 tttccaatca atccgttttc atcctgtgcc caaattgcag ctaacatttt ctcatccttt
     2221 ctgattctaa acagcgatag gagctttgat cgccggatgt gggtcatatc cttctaattg
     2281 gatatcttcc atctcaaaat caaatactga ctgcttttcc tgattcaaaa ctaaagtcgg
     2341 aaatgcgcgt atttctcggc tcaattgttc tttcatttgt tcgatatgat tgatataaag
     2401 atgagcatct cctaatgtgt gaacaaattc gccaaccgct aaacctgttt catgtgcaat
     2461 taggtgagtc agcaacgcat aacttgcaat gttgaacgga acgcctagaa acacatcccc
     2521 actgcgttga tacaattggc agcttagccg tccctcttga acataaaatt gaaacatcgt
     2581 atgacaagga ggcaaagcca tgctaggaac atcttccgga ttccatgcag aaacgatcaa
     2641 tcttctagaa tcaggagtct ttttgattgc ttcgatcact tgtttgattt gatcgataaa
     2701 gccaccgtct tttgtttccc agtgacgcca ttgatagccg tagatgtgtc caagatcgcc
     2761 gtatgtttca gcaaaggaat catcggttag gatcttctcg caaaaaagct tgctctgctt
     2821 tttatattct tcattgaatt caggatctgt caaacatcta tggccaaaat cagtcatatc
     2881 tggtccatta tagtctggac ttttgatgta gcgttcaaat gcccattcat cccaaatatg
     2941 gtttttattt tgtaatagat agcggatatt cgtatctcct tttaaaaacc aaagcaattc
     3001 gcttttgatc aatccaaaag gtactcgttt cgttgttaac aaaggaaacc ctttttgcag
     3061 atcaaaccgc atttggtatc cgaataagct taatgtgcct gtgcctgttc tgtcttcttt
     3121 acgatgccct tcttccaaca attttcttcc taagtcaaga tacgcttgtt ccatcctttt
     3181 tcctcctaat cttctaccca ttcactcaag tattcccacc gtgccatttt ttcttcaagt
     3241 tgtgcttctg tttcagatac gtcgttttgc aattcttgta atcgagtgaa attatctcct
     3301 tgatgattca tctcttcttg gagcaatgag attttttctt caagttctgc aatctcatct
     3361 tcaatcgttt cccattcttt ttgttccatg taagataatt tcttcttttc agcaggttcc
     3421 ttgctctgta tttttggttt cgctttttca gcaggctgct gggtctcttt ttgcttcagt
     3481 aaataactgg acatgtttcc gtaataaagt tcttgttttc cgtttccttg gaatactaat
     3541 aacttatctg ctaccttgtc caagaaataa cggtcatggg aaacagtgat cacagcacct
     3601 ggaaaacttc taaaataatc ttccaaaatc gtcaatgttg ctatgtccaa atcattggtc
     3661 ggttcatcca ataataaaac attaggttgc tgaatcagca gtttcaacaa aaataaccgg
     3721 cgtttttctc cgcctgaaag tttgcctatc agtgtcccat gcataaatct tggaaataaa
     3781 aatcgttcaa gtaattctgc tactccgatt tgactgccat ctgctgtttt tgcttcttca
     3841 gcagcttctt gtaagtaagc aatcatccgt ttatctggtg ccatttcttc attttcttgt
     3901 gtataatacg caagatgaac cgtttcaccg atagacattg taccgctatc caggggaatt
     3961 ctgccagcta gaatattaag caatgtcgat tttcccgcac catttttacc agtaatacct
     4021 agtctttccc gagattgaat caataaatct aaatgttcta gcagtgtttg gtcatttata
     4081 cggtaactgc catctttgat ttccagtact tttttcccta aacgttgtgt cgctaggttc
     4141 agctctaaat catcttcttg gtttacttgg tacaagttct cctttagatc ttcgaaacgg
     4201 ttgatccgtg cttgctgctt ggtgccacgt gcctgtacgc cagccctcat ccaagctaat
     4261 tcctgtttat aaagccgctt gcgcttttct tcttgttcta cttctacacg atcacgttct
     4321 gctttttcta agacgtaagc ttcataattt cctttatact cgtataattt tccaaatgaa
     4381 agttcgaaga ttcggtttgc tactctatct agaaaataac gatcgtgggt aaccatcaat
     4441 agtgcgccac ggtattgttt caaatagttt tccaaccact cgatcgcttc ataatctaaa
     4501 tggtttgttg gttcgtctag taatagtaaa tcgggttcat caatcaacac ttgcgccaga
     4561 ctaactcgtt ttttctgacc acctgataaa gtactgattt ttttatctaa ttcactgatt
     4621 cctaattttt gcaaaataat ctttgcgttg gtatctgtag tccaagcatc ttctttattc
     4681 atttgttctt ctgctttagc ataacgtttt tgcacatcag ggtcttctcc gttttcccct
     4741 agttcgatca atgcttcctc atatgctcga acagtttgga tcaaggggct gtttccttga
     4801 aagactgctt ggagaacagt taattcatcg gaaaattcag ctgcttgaga aagataacca
     4861 atacggtaat cagttggata aaacactgtt tgccgatcac cgtcgctgct atcgataccg
     4921 gcaataatat ttaagagact tgttttaccc gtcccgttcg tcccaatcaa cccgatacgg
     4981 tctttttcgt ggaccaaaaa agaaatctgg tcaaacaaat ctttttcacc ataagttttt
     5041 ttgaggtcag ttacttttaa ttctttcaac atgttccctc cttatcttgg atattgtatc
     5101 ataaaaatag tgaacttcag gaaaattttt ttggtatttg ttctttttgt gttctcaaaa
     5161 aaagaacagg accgattcga tcctgttctg atatgttttg ttttttataa aacttgaaaa
     5221 tttttacctt tttattttat tggactcgtt ttttttgact ttgctcgaaa cgcaaatcgg
     5281 aaagaaaagt tatcagatgg tttgttcatc gtataagatt ctaacaaatt gatattcctg
     5341 cgatagctgt tcgcagaaaa cgtcgtgatt ttttcagcaa cttcgtattc atcaatgatt
     5401 tttctttcaa gataataacc tcttagcatt tttttcgtaa acagactatc ttgctggatc
     5461 atccacgaag aaatcaaatt ccccgagttc atttttattc cttcaagttt gcgttgctgg
     5521 ataaaaaatt gaaccatcgt ttcatcataa atatcagtca agttatctaa actttcaata
     5581 atcaactgag tatttttttg atatgtctct ctgatttctt ggatatcttc atcactaata
     5641 aatgtctgac gatcagcata tcttctttgc caaaacattt tcggatggag aacgattcgc
     5701 attaacctac ggacaacaat gaaccaaaag ccgataaaag acaaaatctg gcttgtgata
     5761 gaatgttcga atcgagaaag aaaacgggag taaaaacgat aaccattaga agaaagcttg
     5821 cccgaacgat aactttcatc caatccatcc cgttcgatcg acaaaatcag tgcttgtatt
     5881 tcttgtactt cttgcttttc atcatcggtt aaatcttcca agtacagatc acgtatccgt
     5941 tcttgataat tttctatcac tgcttcagtg gctaaaaatt cttttgtatt acgatcaatt
     6001 tcctttattt cttttcgcaa aacttcaacg acctcttcta aaatcatcaa agcattcaaa
     6061 tccgtgcttt tcgcttcctc tgtttccgtc agcataggca agacaatgat ccctatgacc
     6121 aaagtcacta agatgacacc cgccgtcaga aacaaaagca aagaccgctg gtaaaacatc
     6181 atattattga ttgaaaatgg caaaataaaa atcgtagcca aactgactgt tccctttaca
     6241 ccgccaaatg ttaagatcaa gatctcgttc aactgttttt tgaacttttt actgccatct
     6301 ttgaatacat aaaaaagact gatagaaatg aatctgatca caaaaagcat gacagagatt
     6361 aatacgataa tcgctaataa aagaccgttt ggatataaac cgtctcccca tactggcgag
     6421 aacacttgcg tcaattcgat tcctaaaaat ataaatacta atgcattcag tgtaaaagca
     6481 atagttgtcc atgtactgtg ggacaagctc gacaattctg catcaaacac agagattttc
     6541 ctaaaaccag aagcttggag aatacctgct gccactgcag caatgattcc agatacatca
     6601 aatgcctctg ccaatacata ggctagaaaa ggaagcaaaa gttcgatcag cagataccct
     6661 gtgacatctt gtgctgatgc tttttcgatc aaatgaatta ttttttgttt gaaccagaca
     6721 agcaaaaagc cgatcaaagc tcccccaata ctagaaacta ctagactcat ccccgcgttt
     6781 actgctgaaa aagatccggt gatcagcgca ccaagagcaa actggaaggc agtaacacca
     6841 gatgcatcat tcaaaagacc ttctccttcc aaaatatgca tcgccttttt agggatattc
     6901 actcgtccag aaagagaact gactgctacc gcatctgtcg gacctaacgc tgctccaaaa
     6961 gcaaagcagg cagcaagtgg gacacttggc aacaggaatg ataacgtcgc acctaccccg
     7021 acaagggtca aaatgactcc tccaaatgcc aagaacaaaa ttgtcccgaa atttttgaga
     7081 atcgaagaaa tatctgcttt ttctccttct ctaaataaca atggtgcaat aatcataact
     7141 aagaacaatt caggttcaaa atcgattgat tgtccccact ctgtcagacc taataccact
     7201 ccaaggaaaa tttggatcag tggagctgga atcattggca aaatgcggct agctatgttc
     7261 gataaggtga tcgttactgc aaatacaata actaactcag caaattccat cgtccatccc
     7321 ctttattttt tattttgtgt aagcattttt aaaaattgtt cttttttctt taaaattttg
     7381 gtttcgacta cttcaacctg tgtttggttc ccctctaatt cacactgtac acgttttgac
     7441 tccaaacgaa gcagatcatg tttcagacgt tccaactgac gatacatcaa tatatttctt
     7501 ttacgcaact tacaaagtat ttgacggcgg tcatccatcg aaagggcgcc atagcgatca
     7561 tgcaacagtt ttttttgttg ttcaaaactc tctctcattt tccagatctc ctatacacaa
     7621 acttccatca ttatagcaaa gttctttttg gatgaaaaat atttcattgt ttcatcgttt
     7681 attgctagtt agaaatttcc ataaattttt caacgcctca ttctattcca aatagtatca
     7741 gatatcaagt aaaacttaag aaagataaaa gtttctgaca aaaggatgtt tactttccca
     7801 ttcctgcctt tcttgttctt ttatcacaac aaaaaaatgg ggctgcgaca aaaatcttgt
     7861 cacagtccct tattctgaat aaacggtgtg gaagagacag cctatcagta gtaataagac
     7921 acatctcttt cacagcctca tttttgcgta aaaatcgatt tattcgaaaa attccccgta
     7981 tacatcttct acacgttgat ctggattgat cacgatatac aagttacctg ttttttcgct
     8041 gatttttgtt gattgatatt gattgtctaa tccttctgtt cctttatcta gatatgggaa
     8101 gtacacaaga tcaggtgtgc catttcggta aaggatttcc attcgctcga tatcttcgta
     8161 tttcaatgcg cgattaaaca tgcccatttc cagtccgcct aggttgatat catgtgtcgc
     8221 aactgcgtcc ccttcatgga agatttcgat tttaaatcca gcacacggat ggatttcaac
     8281 aaattcactg ccattgattc gtccaaaact tgttgtgatt tgtttgatcc aaagatcgcc
     8341 gatatgacgg cggttgatcg tccaggtttc tccatttcta aaataaaatt ttagcgctgt
     8401 catttcttta ctcattaaaa agactccttt atgatgtaat ttattcttgt actacctcag
     8461 tttagcacaa atttatgtaa gtggtcacat cttatgtttt ttcttctaat gacgtatctg
     8521 cccactttaa gtagccaaca aagctgagca gatacagtct agtgtttttt aaatcaattt
     8581 ttctttcgat aaaaaatcca aaacctgttt tttatcattt tttattcttt gttctacgac
     8641 ggctttttca gctaattgca aagcttcact aacccactta ccaggttttt ttccagtttc
     8701 ttctagcaag attttaccat ctactgctaa ttcgcttaat gacttgatag gtaaacgatc
     8761 ataggattct cctacggcat ccatctgagg tgtttcgtca aaatagatca tcaattcttc
     8821 tacttgcagt gcatgttttt tgcctaagaa gtacaattcg aatggttcca aagctccagt
     8881 ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt ggttagaaca
     8941 cttccacgat ttcaaaaagg aacgaatctc attttctttt agatccagtt ggctgatcaa
     9001 taatgcccat gcttggctct cttctttcaa tgggacggat ggtaattcag caaaacgtaa
     9061 aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg tagcactctg tttcaataaa
     9121 tgcatggatt cccgcggccc gttcatttcc caagagcatc ttgatgaatt ccacatttat
     9181 acgttctaca gatattttac ctaatagctc gtggttttct ttgatggcgt agaaggtttc
     9241 ttcctctaat ttgaacccta gttgactgac aaaacgcaac ccgcgcatca ttcgtaaggc
     9301 atcttcatgg aaccgctcat gaggattgcc tacagctcgc aagatctgtt cttctagatc
     9361 ttgcagacca tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa acgcattgat
     9421 cgtaaaatct cttcttttca aatcttctgc caacgaacga acgaattcga catgatctgg
     9481 tctacggtag tcctgataag ttgattccgt ccgaaatgtg gtgatctcat attgttcatc
     9541 attttctaat accaacacag ttccatgttc gatcccaata tctatcgttt ttggaaaaag
     9601 ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc acatcatgga ttggctggtt
     9661 cagcaagata tcacgaacac ttccacctac aaaataagct tcatatcctg cattctctaa
     9721 ttttttcaga actggaattg ccttttggta ttccagaggt aattgtttta atttcatttt
     9781 tccaaccttt cctgcgtgtt tcttgtcctg agataaagac tcagttgcat ggtgactgtt
     9841 tgacaagtcc gtttcagagt cttcaaggcc aggaccactt catcttgttt actcgtcctt
     9901 acgctcaaag cgattatggt ctctttgatt gaatttttcc atgttttttt caaaaacttc
     9961 agttaagtcg atattcaatg aattagccat gatcatcgta acgaataaaa catcaccaag
    10021 ttcttctgct accgagttaa ctggttcaga agcctttttg cttttttctc cataagtgtg
    10081 gttgacttct cttgccaatt cacctacttc ttcagttaat cgagccatct gagctaatgg
    10141 agagaaatac cccaccttga attgctggat atattcatcc acttcctgct gcatactgta
    10201 taaagaacgc tttttatcca tctttttccc tccaaaacta tcttaccaaa actttaacgc
    10261 aaatcctaga taagagcaat tgagaaaatc gaatttccat gctacattta ggaaggaatt
    10321 tctaaatgga aattctgtga aaatacataa ttgaaagctg gaattcatat ggtcaaacga
    10381 aaaacagtaa aagaagttct ttttatcatt ataggaacaa gtatttatgc atttggtctt
    10441 gtttacttga acatcgctaa tcatttagca gaagggggag tctcagggat caccttgatc
    10501 ttaagagctt tatttggtat tgatccagct tatacgactt tattaatcaa catcccattg
    10561 attttactag gaggaaagat cctcggtaaa cgttccttgg cctatactgt tctaggtaca
    10621 ctttctttgt cattcttttt atggatctgg caaagggttc cactagaaat caatttacag
    10681 catgatctac tgatcgtttc tctacttgcg ggtttagttg caggtgtagg aagcgggatc
    10741 gtctaccgca tgggtggaac aacaggcggc agtgatatta tcgctcggat tttagaacaa
    10801 aattacggca tcactatggg gcgttcccta ttggcatttg atatcatcgt cttacttgct
    10861 tcattaacct atatcgactt gaaacgtatg atgtatacgt tgattgtttc gtatgttttc
    10921 agtcgtgtga ttgattcgat cttagatggc ggatattctg caaaaggtct acttgtcgtc
    10981 tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag aggtgtgact
    11041 ttcctttctg gagaaggcgc attctctggt aattcaaaaa aaatcatcta tatggtcgtt
    11101 agctctagtg aattaactga agctaagagg atcgtccacg agattgacga aaaagctttc
    11161 ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct ttacttattt gaagccacag
    11221 acaagattac tgaaaaaact ataagaaaaa agacaaagat aggctatgag gaaagattcc
    11281 acatccagct gacctttgtc ttttttagta ctatactatt tttaactaat tgttatgttc
    11341 acttacccgg ttacttttct tttgtgtctg caaaaggatc tttcggttaa cttcatcaga
    11401 taatttcaaa aaagcatccc tatctccatc ttctagtgct tgatcgattt gagcataaag
    11461 catttttagt tttgcctctt cttctttttc ttgaaaatat cgttcaatta attcagtatc
    11521 ttcttcactg actgaagaat cccaagaaga aaatggattg tcttctaaga tagatagata
    11581 ttctcttgtc tgccaagcat tttcgaatat acattccaca tataaaggct ttttccaatt
    11641 caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct gcaaaaacaa
    11701 tttcatgggt tccccttcaa cagatagatc actgatctgc agtcctcgat ccgcaaaatt
    11761 cgccccttca acaaagtgga cattgcttaa aatcgcttca tgattggcaa gataattcaa
    11821 gatccaaaaa acttctcttc ggctaaaaga tacatgattg accatccacg ttaaaaaatt
    11881 cttcttttcc cgtacatcaa ttagcatttt tctcacctct ctgttaggtc atctaataat
    11941 gacagtgcct ccatatctcc tggttcaagt gttaaataat aagtcaataa ttcttgcgct
    12001 cttttcagct gcccttcttc tcttaagaaa aaggcatact ctttcaagaa atctggttca
    12061 tgtgatagtt catgataagc ctgttcatag tgtacagctg caacggtaaa gtcttctaat
    12121 tcattataag catgggctag gttccattct gcataaggat tagcagtttc ttccatctga
    12181 ttgatcactt gtatcacatc ttcatatctt tcatcatcca agtaaagatt acttaaagta
    12241 agtaatgttt catcttgttt gtctcctact tctaaagctt tcagtaaaag ttcttctgct
    12301 cgctgtctat catgcagacg gaaagcattt tccgaggcca ggtgataaag ttcgacttgg
    12361 taaggatttt ctttgattcc ttcttctaac actgtttgtg cttcttcaat catttcttct
    12421 tcttgaagag cttgtcctag atatagatat aacgattgat aatgcggatt aacttctcga
    12481 agttcttcca gatagttgat tgcctgctga ttttctttca attgaagatt cgtaaaagct
    12541 agctggaaca gtcgatcatc tgtcctttct tctttcagtg cttcttctaa aggtacgata
    12601 gctgcttcga attctccttg catacttaag gattgtccta aacgttcctg catcgagatt
    12661 cctgaaattt catttatccc gcttgccaac aatgtttcat aaaccactgc tgcctctttg
    12721 aaacgatcaa ctgaaaaata caattcagct agcgcaaact ggatcaatgg ttcatcagga
    12781 agcaacccag ctgcttcttt caattttgct tcactcactt cagggattcc gataacttgg
    12841 tacaaatcag ctatcgccaa taagctttgc ggatagtaat cactttgctt atccactttt
    12901 tccaggtatt caaaagctaa gtcgatctca ttatcttcga ttgcaatttc tgctaacggg
    12961 atgttcagtc cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat ctgttttgct
    13021 tcgatcaaaa agccaatagc cagcaattct tcgcctagtt cagctaatat atctgctggg
    13081 tcttctttta atgctttttc taaaaagatt tgtgcttgtg acaagtcttc atctgataaa
    13141 gcttgcaaca ttttttcgct atttgtattc atttcaattc cctcttatct cactgacgat
    13201 ttgttgaaat accgtcattg ttctcaataa ttctttttcg tttggatgtt ggtatccgtc
    13261 tattatacct attttttcta aaacaagtat ttttttgctt ttttctgcca aaaaaagaac
    13321 tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat agccaagcaa
    13381 gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt ggagattcca
    13441 catgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta gatggccatt
    13501 ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt tttctgcctc
    13561 taattgataa aaatagatca tttcttcgat catcccggca aacggccgag tcgttaattg
    13621 ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac aaatcattcc
    13681 gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct cgctgattgt
    13741 ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac gatttggtag
    13801 ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg atacagggat
    13861 catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca gatccatgat
    13921 cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc gtttgacgaa
    13981 atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac aaatatacca
    14041 gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca aatcataata
    14101 gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta gctgactgac
    14161 tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat caagttccat
    14221 acgcatccct cctcttctta tttttatttt accattagta agcaaattct agaaaagata
    14281 agagcaaagc tcacctcata acttgcaaat aaataaaaat taaataaaat acatgtgttt
    14341 tctatgtatg acatacggat tcggaatctc tgtctcttat ttattccttt tctgctagga
    14401 aataaaaaga aaagcacttg tagccctaga gccacaagcg ctttttcaac tgtcttattt
    14461 aacagcgtct tttaatgctt tacctggttt gaatgcaggt actttgctag ctggaatttc
    14521 gatttcttca ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc gaacttcaaa
    14581 gttaccaaaa ccgattaatt gaactttttc gccgttagct aaagcatctt gaatagttga
    14641 aaatacagca tctactgctg cagttgcatc ttttttagtt aaatcagtag ctgaagcaac
    14701 tttttcgatt aattctgctt tgtttgccat ggatagattc acctcctcca caagagatat
    14761 gatagaaata tcatttagtt aaaactttct gagtggtcca gaagtttaaa aagaaatatt
    14821 gattaacttc aatattctgt tatcacgata tcatatgaac cgcttaatag caaggttttt
    14881 accaattttc acatgctttt tttagtcata gaaacgtaac aaacgaaact gtctttttct
    14941 tttgaaaaaa aaacgtcgat atggtaaaat gcctattttc tcctacgagg gataattcgg
    15001 atggacgtcc cttcaaaagt aaatgctttt ctgatttgat tttctaagaa acgttcataa
    15061 gaaaaatgca tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg tttgacagcg
    15121 acttgtgtag cataaaaaat cttcaggcgt tttcctttat ctgttggtgt tggattgatt
    15181 gctacagcat ccataatgat atcattcaac acagccgaag agatacgaag attctggttc
    15241 atacttactt gttcgatcat ttcaggcaga cgttccaacc gttgtttagt cactgcagaa
    15301 acaaaaacga ttggagcgta atcaagatac cggaattcat cacgaatctc ttgttcgaaa
    15361 tcacgcattg tattggtttc tttctttaca agatcccatt tattcacgac gataataatt
    15421 ccacgaccag cttcatgggc gtaacccgct actcgcttgt cttgttcccg aatcccttct
    15481 tcagcattca gtaccatcaa aacgatatca gaacggtcaa tggcacgcat tgcacgcatc
    15541 acgctgtatt tttcagtatt ttcgtatact tttccgcgtt tacgcatacc agctgtatct
    15601 atcattaaga atttctgacc actttcagaa acgaagtggg tgtcgattgc gtctcgggtc
    15661 gttccttcaa tatccgaaac aattacacgt tcttctccta aaatcgcatt gatcaaagag
    15721 gatttaccaa catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc gtcctcttct
    15781 tcagtctctt cagagaaata cttcactgct tcatctaaaa tatcaccaat ccctagtcca
    15841 tggcttcctg aaacaggata aggatcgcct agccctaagg aataaaattc atagatatcg
    15901 tttctcattt ctggattatc tactttattg actgctaaaa tcactggttt attacttctg
    15961 tataaaattt tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc gctgactaca
    16021 caaatgatta catctgcttc ttcaatcgcg atttctgctt gatgcttgat ctgatccata
    16081 aaaggttcat cgccaagatc aattccgcct gtgtcgatca cgctgaattc tcgacctaac
    16141 cattcacctt ttgcataaat acgatcacga gttactccag gagtatcttc gacaatcgag
    16201 atacgctcac cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg gcggccaacg
    16261 attgcaattg ttggatttgc catattattt cctcctcaca agtttccaat ctcacttagt
    16321 ttaacgaatg cattgccaac tttcaagcat agattgtcaa ttaaacgaag tatttttatt
    16381 ccttattgaa atactaatcg cttttcaaaa gaaaaactga gacaaaagca gctgctttca
    16441 tctcagtcac agatacaaaa tcaattattt ttcagaatca tcggttgctt tttcttctac
    16501 ttcttctttc aacgcatcac ctaaaatgtc tcccatagta aatccagtat tttcttctgg
    16561 taattcataa gactcaacat tttttggttc ttcttctgaa gctggttttt cttctaaagc
    16621 tttgatactc aatgcaatac gatgttcttc tggatggatc tctaatactt tcactttcac
    16681 ttcgtcacct tcatgaagca cttcatgtgg tgtcgcaata tgtttatgtg aaatttgaga
    16741 gatatggact agcccttcta ctccagggaa cacttcaaca aaggcaccga agcttgtcaa
    16801 acgtttaact gttccgtcta atactgtgcc aactggagct tttgtatcga tttctgacca
    16861 aggtccaggc tgtgtgtctt tgattgacaa ggaaacacgt tcttgttcag gattgataga
    16921 caacactttg acatttacat catcattgac agccaataca tcacttggtt ttgctacatg
    16981 actatgtgaa atctctgaga catggactag accgtcaatt ccgcctaggt cgacaaatgc
    17041 gccaaaatca gtaagacgtg caacacgtcc actgagaaca tctccctcat ggatagttga
    17101 aagaatttct tttttctttt cttctctttc gttttccact accgctttat gcgataagat
    17161 caaacgattt tctgatggtt cgatttcgat gattttaaag ctcaatgttt tgcctttata
    17221 gtctgaaaag tctgcaacaa aatgatcttc caccattgat gcagggacaa aaccacgtac
    17281 accgacgtca acgactaaac cgcctttgac aacatttgta acaggtgctt cgatgacctt
    17341 gccctcttgg aaatctcttt cgatatcttc ccaaactttt ttagcatcta aacggcgttt
    17401 tgaaagtaga tagctgccgt tttctttatc tttgccgatc gtactgataa cgactaaatc
    17461 taatacatca ccgactttta ctacttcgct gatatcttct accggcaaag ttgataattc
    17521 ttttgccgga acgactcctt caactccagc cccttcgatt ccgacaatca cttgtttgtc
    17581 ttcaatgact aaaacttcgc ccttaacaac atcgccaact tttacttctt gtacgctttt
    17641 cattgcgtct tccattgact ggttgttctc ttgattttga tcaaattctg tcataacact
    17701 tgtcctccta accaacattc tgccgtaagt acggtactgt ttttagttta attgattatt
    17761 caaaaaaaat aaagcgattt cgttcgtttt ccttgaaaaa acaattgttt ttcatttttt
    17821 ttcttaattt aaaaaagaag ataacctttt ttcaaaacaa cagcttttat tgctgcaacg
    17881 acttctggaa tcgatttgcc agtcgtatcg atacgaacag catcctctgc ttgcttcagc
    17941 ggagagacgt cccttgttga atcataatgg tctctttcct gtatctcttt cgtcagtgtt
    18001 tcaagatcag tcggaattcc tttttcttgg ttctctttga aacgtctttc tgcacgctca
    18061 cttacgcttg ctactaaaaa gattttcact tctgcattcg gtaatacagc tgtcccgatg
    18121 tctcttccgt ccatgacaac gccaccgcgt tcccctattt ttcgttggac agcaactaat
    18181 tcttctctga cttttccatg tgcagagact tcagaaacag cttttgtgac atctggttga
    18241 cggatttcta aagtcacatc ttttgatgct atgaatacat gctgtccatc ttcttgttgc
    18301 gaaaaagaga taggatactt tttgatcagc gaaactagcc cttcttcatc tgaaaaagat
    18361 acattgttcg taatagctaa ataagttact gcgcgataca tggcaccggt atctgtataa
    18421 atataattca attcttttgc taatatcttg gcaacggtac ttttcccaga tgaagcaggc
    18481 ccgtcgattg caatactgat ttgtgtcatg atataactcc tttttctcag aaaaaaagcc
    18541 gcaaagcgcg gctattatta tttaattctt aattcttgac cagggtaaag cataaagttg
    18601 tttggatcta agccattcaa ttcaaacaat tgatcagtgg tgatccctgc acgctcagcc
    18661 acttgttttg gaccttcgcc tgcttgaaca gtcgttgttg aaccggctgc agctgatgaa
    18721 gaaggtgttg tttctgctgc attgctgctt gaatcccctg cttgataagt tgcctcactt
    18781 gaggaagtcg gtgttgtctc tgctgcaata ctgctttctg acgttacacc tgtatcttga
    18841 gacacagtag attcttccgc gattgtacta gattctactg ttgaagtctc agttgactcc
    18901 tcttttgttg tacttgtaga actttctact actgatgtcg aagtttcaga agaagttgct
    18961 gctgttgcat tgttgcttga tccatttctg atccagaaat aagcacctgc tggaatagcg
    19021 atacataaga ccagtaaaat caaaaatagc gaaagaaata atgtatttcc tttcttttgt
    19081 tgtctctgag aacttcttga tgaaacttca tcatctgtat cgtagattgg ttgttcccat
    19141 ggttcttgtg tttcgttatt ttctgtagat tgatgtctat cttttctgct cactgtttta
    19201 cctcctaaac tctatcgtgt gtattatacc ataggaaaaa gtgtcttgtc ctaaaaaatc
    19261 aatgttccat gtttttttta aataaattta gtaaaatcgt ttcccaatga agttgttcag
    19321 tcttagaaaa aagcttcctc tttggcagaa gttctgcttt tgctccatca atatcacaac
    19381 aattctgcgg cttttcggag agtttttcgc caaaataagc taagatgaat tttctgcggc
    19441 agttttcttc attaatatag cgaagcatct gctgcaggcg gaattctttt tctttttcct
    19501 gacgtttgag ccgttccacc caattttctg gtttttcaga ctgctggatc agctccatcc
    19561 attttttttg cagctcatca aagggagctt gctttgtggc atattccaaa taaagctcaa
    19621 aactttgtcg ctgttctcta gaaagttgat taaagaaata atggatacgt tcatccccac
    19681 tttgataaaa aagaattgct gcactttctt cctgatctct gcctgctcga ccgatttctt
    19741 gtacatagtt ttctagactg tctggcaaat cataatggac cacataacgg atatcagatt
    19801 tatcgatccc cataccaaat gcattcgtag caatgagaaa ctgcagctga ttttttacaa
    19861 actgctgttg aagttgtctt ctttgagctg catcaagacc accgtgatag tatccaactg
    19921 taaaacgttc cctgaataaa tgatacaatc gttcgacttc tttttttgtt gcacaataaa
    19981 tgattgccgc tccttgtgct ctttccatga agtgttcaag ttcttgttct ttttgctgcg
    20041 ttttccgaac aaaaagcgag atattttgcc gattgactga ttgtctgatc tcttttggtg
    20101 gtcgatcaaa tagtacatga cgtatatctc ttgctactaa atctgttgct gtcgccgtta
    20161 gtgccagtgt gaccgggttt cctaaaagct ttcggatttt tcctaactgt tgatattctg
    20221 gacggaaatc aactccccat tgagacacgc aatgagcctc atctactaca tataacgcaa
    20281 tattttgttt tttcagatgc tctaaaagag aaggattagt caacatttcg ggactcaaaa
    20341 acaaaaattt atattgcgat agatgacgga ggacgtatct tctttcagat tgcgaaagta
    20401 aactattgaa agcagctact cgtttctccc ctcgtttttg taattgcgtc acttgatctt
    20461 ccattagaga aagcagcggt gacacgatga tgaccagacc ttccattaaa taccccgtca
    20521 attgatagca aaggcttttt cccgttcctg taggcaaaat agcgagagta tcttctccat
    20581 caagcaacgc ctgaataatt tcttcttggc cgggacgaaa tgaatcaaat ccaaaatatt
    20641 tttttaattc atttattaac attctgtatc gcttcctttc tcaatagata gatttgatac
    20701 aaacgaaatt ctccataatc taattgtcca tctacattcc attcgctaaa gcgccactct
    20761 cgcacggaat ccttatttgg ttccaaacgt tcgattgttt ctgcagagag aatccactcg
    20821 tatggaaaat catcaaaaaa caaggcccac tcaataagat gatctgttac tgttcctctt
    20881 tttaaatgac ggattgccat gatctggtca attgtctgac cattcataaa catctgcttg
    20941 gtgatcatca tgctttgatt gaaattttgc tgtaataatg aatcgatcaa agcgtgccaa
    21001 agcgaatctg gccgtttttc gatttgtgct aacaaaaggt ggatacatct gctttgttgc
    21061 agataaacct ctgactcatc attgtttttt gccagctgat aaggaagcag ccctgttcgc
    21121 tcatttccag ataattggtt agctaaaaaa tctgctgctt cttctggtat ctccgaaaac
    21181 agctgcacca aactctcata cgcagtatca attaaaattt cacgtggcaa ttggctttca
    21241 ctaagccatt tttttagttg gaaagtataa aaaggacttg tctctgctgg aagatagtct
    21301 tgaatacccg ttgccagatt agaaattact tgaacagcaa atttaatgag ccgccaacta
    21361 gtttcccctg ttctgccata gcggtcaaaa cgcaaaccag tatgttctat ttgatgttcg
    21421 gacaacatat cagcccctgc aggcgttagc tttgcttcat tttcatttat ttcgatccaa
    21481 ccttgctgcg caattttttg caaaatttga taaaacttat cctgcttcaa ttctggaaaa
    21541 ctgttatgga taaacaaaag ttcgtgggta aatccataaa taagtactga agtcgttcgt
    21601 tttcccacaa ggagttggta aagagaacta atccgcagct tgtgtcctga tgaaaatagc
    21661 gataaaataa aaacaatcat attattctcc ttaagttagg atggttagaa atgtcactac
    21721 tatgtaaact tgtacctgaa cgctgtattg catgcggtct ttgtcaaatc gaagcgccag
    21781 atattttcga ttatgatgaa gaaggaatcg ttttatttgc ccaagaacca gaggctcatc
    21841 aacagttcgt gatgccggaa gaagaaacat atgtaaaaaa agcgtatcaa aaatgtccag
    21901 ttcgagcgat tttattagaa aataaaatat aaatataaac gaaactttaa aaacgtacac
    21961 taagttctct aaacttgaaa ataagccggg atctgtttct cttgttccgt gaaatcccga
    22021 cttattttga aagattcatt tgctatctat agttaattga ctagtttttt cacggaccct
    22081 ataggaccca tttatattat ttcaactttt tcagccagtt ttccaatgta ttgacgatca
    22141 cagtttgctg gtctgcgtta gaaatttctg cctctccatc tcctttttgc tcaccatagc
    22201 tgccaaaacc tccatgattt ccgccattga ttgatacata ctctgttctt tccggtaaat
    22261 actgcttagc ttcttgataa gcttcctgat tcaatacttt atcttttgtc gccgtgatcg
    22321 ataaaacagg aacagctgat tctgtcaatg ttcctttttt gtcgggataa cttgccatga
    22381 agaatacccc ttttaaatgt ttgtctgaat gattcttatt gacataacga cttgccatta
    22441 cgccacctaa tgaatggcca ccgatcacat aatcggaatt tggaaaatct tctttgacct
    22501 tatccgcttt gtttccgccc atcactgcta gatcaagtgg cattttgaca atagcgactg
    22561 aaaagccgtt ttttgctaaa tcttgcgccc aaatactata actttctggc tctacgagag
    22621 caccaggata aaaaataagt aatggagact ttccatgatt ctttttctct ccttcaaaaa
    22681 ataagtaatc ttctttgttt tttccttctt ttaccaacac ttgcgcttct gacgatggtt
    22741 cgtacgtatt ttgctgtata tagtaataac cggctccacc gagtaatcct aaaatgatga
    22801 ctaagccaat tagtattcgt ctaaatattt tcactttctc ccaccttttc tatctgagtg
    22861 accccttttt cttaatcaag tatgattata ccttaaaaaa ataagagaga aatgcaaact
    22921 ttccagcatc aaataaaa
//
LOCUS       DACDRN010000040        22875 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDRN010000040 DACDRN010000000
VERSION     DACDRN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.040"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(3..2060)
                     /locus_tag="IWJ80_001783"
     CDS             complement(3..2060)
                     /locus_tag="IWJ80_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP9461499.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(2174..2386)
                     /locus_tag="IWJ80_001784"
     CDS             complement(2174..2386)
                     /locus_tag="IWJ80_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461500.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            2573..3040
                     /locus_tag="IWJ80_001785"
     CDS             2573..3040
                     /locus_tag="IWJ80_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461501.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            3037..3279
                     /locus_tag="IWJ80_001786"
     CDS             3037..3279
                     /locus_tag="IWJ80_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461502.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            3297..3599
                     /locus_tag="IWJ80_001787"
     CDS             3297..3599
                     /locus_tag="IWJ80_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461503.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            3671..5725
                     /locus_tag="IWJ80_001788"
     CDS             3671..5725
                     /locus_tag="IWJ80_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461504.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            5725..8337
                     /locus_tag="IWJ80_001789"
     CDS             5725..8337
                     /locus_tag="IWJ80_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP9461505.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            8334..10865
                     /locus_tag="IWJ80_001790"
     CDS             8334..10865
                     /locus_tag="IWJ80_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461506.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            10917..11249
                     /locus_tag="IWJ80_001791"
     CDS             10917..11249
                     /locus_tag="IWJ80_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461507.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            11250..11843
                     /locus_tag="IWJ80_001792"
     CDS             11250..11843
                     /locus_tag="IWJ80_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461508.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            12006..13700
                     /locus_tag="IWJ80_001793"
     CDS             12006..13700
                     /locus_tag="IWJ80_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9461509.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            14388..16265
                     /gene="ltrA"
                     /locus_tag="IWJ80_001794"
     CDS             14388..16265
                     /gene="ltrA"
                     /locus_tag="IWJ80_001794"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9461510.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            16396..16773
                     /locus_tag="IWJ80_001795"
                     /pseudo
     CDS             16396..16773
                     /locus_tag="IWJ80_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            16773..17978
                     /locus_tag="IWJ80_001796"
     CDS             16773..17978
                     /locus_tag="IWJ80_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9461511.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            17994..18638
                     /locus_tag="IWJ80_001797"
     CDS             17994..18638
                     /locus_tag="IWJ80_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461512.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            18649..19455
                     /locus_tag="IWJ80_001798"
     CDS             18649..19455
                     /locus_tag="IWJ80_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461513.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            19477..19707
                     /locus_tag="IWJ80_001799"
     CDS             19477..19707
                     /locus_tag="IWJ80_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461514.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            20220..20516
                     /locus_tag="IWJ80_001800"
     CDS             20220..20516
                     /locus_tag="IWJ80_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461515.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            20518..20940
                     /locus_tag="IWJ80_001801"
     CDS             20518..20940
                     /locus_tag="IWJ80_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461516.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            20959..22551
                     /locus_tag="IWJ80_001802"
     CDS             20959..22551
                     /locus_tag="IWJ80_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461517.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            22572..>22875
                     /locus_tag="IWJ80_001803"
     CDS             22572..>22875
                     /locus_tag="IWJ80_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461518.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKS"
BASE COUNT         8356 a         3596 c         4649 g         6274 t
ORIGIN      
        1 ttttatttga agattggatt cgcactaata aatacattat ctacgccaat tctaccagca
       61 gagtcaacta caaaattatg acctaatccg agaatacttt ggttattata aagtaaattt
      121 gcattatggg cagggctatt tttccatcct accatggcat cgatagcacc atcgtctgat
      181 aaaaggtcat caatatacag cccagtttga cttatgtttt ctcccttagc accgtagcag
      241 cctaagcttt ccaataaatc ttggaaagta ccaccaccag ggcgatagtg actgaagctg
      301 acttgtaatt ctttagctcg aatatctgca gcctgttgaa caatatcatt gaattgcatt
      361 ggggcaacac cattttgttg gcgataatca tttaaccaag caagttcttt ttgacgcata
      421 aagttacgta attcgtctaa tgaaatcttt ttatatttga atgtgattgt tttattttga
      481 tcgacgttat taatggatcg ttggccgttt ttatccacgg taacggtagg atcgtcattt
      541 ctgcacaaga aataattttg atcagtcatg tttttccaag gagtagcaaa gctatcccct
      601 tcgtatactg tttttgattc actagagaga acagcgttgg tatcagcatt tacatgatta
      661 acagtcacat taaatttacg agtgtagttg aaaatataat tttgactttc agtcacattg
      721 tttaaggtta ctgaatttgt tggttctacc cgatcgtcta aaacatatcc ttgaatagaa
      781 ggagcagtta aagtgacact gtcaccgtga gtgacattga ctatctgttc tttcaaaata
      841 ttaactcgtc cttcgttttt gtagttgtta ttgcacaaca aattgatttg atgcacttga
      901 ttataattga atgtgattgt tgtattacct gtgattgttt tgctttgagt atcattacca
      961 atcactttgt agctaaaatt atctttagcg ccttcgctat atttaaatgc ttttgcctta
     1021 gcagtgtaag tagaaccaga agttactttt gcttgatctg tagcaatcac ttgacctgtt
     1081 tgattgtcaa tatggttaat agtaaccgta tattcttttg gtgtttcagg tttgtgttgc
     1141 aaccctttaa atgcagcatc aagactattt ttagcattgt ttacttgtgt ttgagaaacc
     1201 ttagaatcgt ctagtacttt tttagcattg gataaagcag tctgaaaatt attccaagat
     1261 tcttctgtaa aatctccttt agcagttgat tttactcggt tgtataaagc ttgtaattca
     1321 gctttattca cagtaggaac tggttttttc tttaatcctt tgtaagcaga atctaattta
     1381 tttttggcac tatccacttg cttttgagtc gcttttgagt catttaacac tttcttagca
     1441 ttgtttaaag cggtttggaa tgcgttccag gtattgtcgg tatagtcacc ttttttcgtt
     1501 cccttaacgc tcgtataaag agcttctaga gccgatttat tcactttggg tttagaggtg
     1561 tcagcgccat accaaccgat accttcgtat ttccagccat agtctttggt taaccattgt
     1621 acttctttcc aacttgcagt gtagtgatgt gagccagatt ttacaccagg aaggaaaagg
     1681 cggtagatcg gtttattatt tttttcggca gaacgccagg caggcccttc ataagtccag
     1741 ccgtaatttt tagtcaacca tttcacttca tcccaactag ctgtgtaatg gtgatcacga
     1801 agacctttat tgtacaaacg atagacaaga tggccaccgc tagtcggtgc ttcccaggcg
     1861 gggccttcat aatttcccca ttttatcttt actagatggt cacgttcttt tttgctagag
     1921 gtgtaaaaat gttcattatt atttggattg taaaggcgat acatgacgtt agttgaagca
     1981 gcgtcaacag atccagcaag actaagtgag gcagcaaatg ttccaatagc taaaacagtt
     2041 tttggattaa ataatttcat tttttcaact cctaaattta attaaaaatg tttacatgat
     2101 tattatatat cggctaaccg atatagtcaa taaaaaataa gaaaagcctt atataatttt
     2161 ggcttttctt attttatttg ttagttgaag tattaatatc aacgatttct tcagccaatt
     2221 tatttaattt catagcattt atgaatctca tgttgtctat actagagcgt ttgttggcaa
     2281 ggtcacttac tgtagttaag ggtacgccag attctatgga tatagaacga actgatttac
     2341 cactatcaaa cagcaaccat tcgattttct ttttatcagc gtacatttca atgctccttc
     2401 ttgttttttc tttattatag ctcttttttg taatgaatca caatatcaag agaaaaattt
     2461 tctatagaaa ggtggatttt tatgagtatt cgttctccgt gtatccaaat gaggtttgtt
     2521 gctactttcg ttatttgata tactaaaaat aaacaacgaa agaaggaata aaatgaagaa
     2581 aaagcaacta tggtttgtct ctatgtttgc aatatttttt attattttaa gtggatgttc
     2641 aagtaataag aaatcggaag ctaaagaaga taatagttta ttgcaaccag gaactgtttg
     2701 gaaagaagaa gtaggtggtc ttattcataa tttaaaaatt atagatgata ctacctggga
     2761 atattctgaa agtgtatggc atccagatcc tgtacaaata acagttaaga ggcaaaaaga
     2821 ctatgaaggt ttagaaagat acaaaatagt agattctgca ggcattaggg aatttataaa
     2881 caaacctgat tcattattta ttgttgttcc ctatgagaag gatggcgtaa aaaaaataat
     2941 ttttcttgga tctagcaagg atgagaagcg aacgaaagag aaattaattc atgatggatc
     3001 agagttaaat aaatacaaat tacaaaagac tagtgagtga ttgctagtct ttttatttat
     3061 cctgaaggag gaatcacagt ggcaaaagtt aaaacaccat tcgaaaaagc cgcgtctaaa
     3121 atcttagaac gccgtggtgt ttctgaaata cagtggaaaa aagaagttct tgaaaaagca
     3181 caatttgacc tattacgagg aaaagataag gacttagaag aatatatcta tcaacgtgaa
     3241 cgtgaaaaac ttgtattcga tgaaattatt aaacattaaa aggagcgaat aatcttatgt
     3301 ttgaatcaat tacaatgaat tttttaactc aaccgaaagt agtagctgca gttgatattt
     3361 ttagagcatt acaaactgcc gcaactagtt tgaaaaataa tctgatgatt tttgctgctg
     3421 ttgcggcagt ggcttccatt gctcttggag gtattttctt cttctttggg cgaagaggag
     3481 ctgaaaacgg gaagacacac gtaggttatg tattgttggg ggtaggagtt attttagctg
     3541 ccgcttctat tgtagtttca atgttctcaa tgtttggaca aaccccacaa ggactataaa
     3601 aaaaacaagt aggaggagta aaaagtacgt ggctactttt tactcctttt tttatgaaag
     3661 gagaatattc atgtttttta cgatttcagg aaatgcaaaa ttggataaag agttaataag
     3721 aaaagcaacg aagaaagtgg gaaagcgtac ttccttttta ccgaaaacaa ttaaatattc
     3781 tgtcattgaa acggaagaac agctacatgt gttaggacaa gtgttagaac aagagaatca
     3841 atcttctgtt aaattgactc tcacgttaca tgataaagaa ggaaaaatag aaaaggctgg
     3901 cacagaatac gtttttggcc cactggatta tcaaatttca gaaggttttt atcctacatt
     3961 gatagattta atccgccttg atgtgtttaa agatacggat gaaactatga gtgtggaaga
     4021 gtcttatcga aacgaaaaac ttttacaagt tttagaagaa gagttagaga gcgaccaaag
     4081 aaaagtgaat caagtgagtg aagtcgaagt aaatgaagaa gtaccatggg aacgaccaat
     4141 ctttccaaag aaggagtcaa aagaaaaaga gacagtctat gttgacgaac aagctgatat
     4201 tccgactcag aaatcgatat ttgaggaaga gaacgatgtg atgggtcaat tggaggtaga
     4261 tccctttgaa gaaacaatgg aggatgaaga gacggacgaa gtagaagttg atccagttga
     4321 agctaatgaa gagtcaacat ggactgaagg ggattttcag gaatatattg aaccacttat
     4381 cagtgaagaa gtagaaaaaa acgaaacaga gatacaaaag gcaactgtat ttgattatat
     4441 tttggatcaa tatccgccag agcatgaatg gatgaaagaa aaaaacaaat tatttttaaa
     4501 tgagctttat tcgaaatatc aattacccca aacctatcaa gcatttttag agagtaaaga
     4561 agaattaatc tcacagggta aaggcacact tttagaacag ttagagtctg tccaatcaac
     4621 aaattggcat gagcttgcac aagaagattt attagccacc tttgaagaac gtcaacaaga
     4681 aacagatcaa gaaattaatg agtatgcgac ggaacagtcg tctaattggg agaaagtaaa
     4741 agaggaacta aataaagaag aacagcgagt gattgaagaa gaagtacaaa aagtcaaaga
     4801 gcggtacgaa aagaaacgcg acaatgctta ttccttgtgt gtagaaaaga tcaacaattt
     4861 tactaagacg aatcaagaag cgttatcgaa agaaaaagaa aacttgttaa aagaacgtat
     4921 taaagaacga aaagaagagt tctaccgcgg tttacgtaca gataagctaa agattagcaa
     4981 agaattaaat gaacaactga ataagctata tcaaacaacg actgaagttc ttatggaaaa
     5041 acacgaacag atccagcaag aattacaagt acaaattcca aaatggaaaa aagagtatga
     5101 aattcaacaa caagaattga aagaacaaga agaaaaacaa cggcaaaaag aaaaagagcg
     5161 tgaacaatta gaaataagaa aacgggaaca ggatcttgcg gaacaaaaaa tggcgataga
     5221 aaaacaacgg ttagagcaag aaaaggaaaa agaagcagct gctcgtaagg aacgtcaaga
     5281 acaaatggat acattgcgtc aagcacagtt gaattatcta gcacaacctc aaccagtagc
     5341 tgctgttcaa acgccagtga acaaccaagc aaatttaact ccttcacccc agcgttcctt
     5401 taacggatgg atggtgggat gtattgcaag tttaagcttg cttctaggtg gaggaacagt
     5461 atttgctttt aatcatatca atcaatcttc acaggctgat tctgtagcca caacggcgtc
     5521 aatcaaggaa gaagcccagg aacaagcaag aaaagagtat gaacaacaat tagaagaatt
     5581 attgaatcgt accaatcagt caacaacaga gtcaagcatg gaaaatagca aagagagtga
     5641 ttctagtaag gctaaagaag gatcaagcga ttcaactgta caatcaaaca cttccgaaag
     5701 ttcagaatca aatgaggtga aataatgcgt gttagagaaa caacgaagcg aaaagacaaa
     5761 cgattcaatt tagaaaagaa taggtatctt tctcatttct atcgctatac tccctcagga
     5821 gaagagctac cagaaaaaca tgacaaacgc atttcgaaaa agatttttta tagtggactt
     5881 gtatacctcc ctttagagta tttagtaaca agagtaccag aagttttcaa ccgattggaa
     5941 attgttaagg gaagcttaca aggaataaat ctgtttcaat tacaggaaat gccacaggtc
     6001 tttcattttg attcagctgg tttttggaat ccatttactc ctacaactag tttgggtaca
     6061 cgtttagcaa tttcgacagt ttgtgtggtt ccagctgtac ttatgggatt gaaaatggac
     6121 atgtggtttc gtcctctagc agatggacaa aaaggggata atcgattaat gaccttaaaa
     6181 gaaatccaac agatttatcc gcaaatacct gaaacaaaaa ctacttttcc aggttatgga
     6241 ggtattccaa tcacacatta caaaaaatat tggtacattc aaacagatac tgttaatacg
     6301 tgtattgttg gtacctctcg atcagggaaa ggtcagattg aagtgttagc caccattgac
     6361 aatttatctc gtgcagaaaa acaaagttca atggtcgtta atgatccaaa aacagaatta
     6421 tatgttggta gtaaagatga actagaagct agagggtatg atgtatatgc ctttaatatc
     6481 accgatccac tgcaatcaat gagtgataat ccattggcac tcatcgtgaa atactggaaa
     6541 cgaggagaca tagatactgc aacacagtta accaatactt tcacaaatac gatttattat
     6601 gatgccaacg cttcagataa caaatacttt aacgaaaacg cgcagaaagc tgtcaatgcg
     6661 cttatttttg cgttgttaga acaagctgat caaacaggag attactccaa attgacaccg
     6721 aataatctcg ttgagcttct ttctgagatt ggcggtttta attattatcc agatccagac
     6781 aatgaattca aacagttgaa tgccttagat gaatttatga atcagttgcc acctggaaac
     6841 attgcaaaaa aacaatatgg ggcaacgaaa attggatctg ataaagcgaa gggaaatatt
     6901 ttatcaacgg caatcacggg attgaatcca tttactctaa cgaaaattgc aaagatgact
     6961 tctcaatcaa cgattgatta taaatcggtt gggtttccaa aatacttaga tttgaaatta
     7021 catgaaagtc ttttaaatca acgagtcgta gtagaatttt atcataaggg aaagaaattt
     7081 catgaagaac gtgtcaaagt aggctataaa ggatttagtg aaatcaattt tgattgtcat
     7141 ttagttgcag gtgatcaagt ttgtttaaaa tacgatcatc aaggaaaaga atatgtggct
     7201 tggtatgaat tttctcaaag agtattgaaa gacgaagagg gtaatatcgt ttatcggaaa
     7261 aaacgaggag aaacccattt gccagaatta gaaaaagaag ccgttctaac attagatgag
     7321 gaaaaaagta acttattagt agagtctatt cggatgcact ataccgataa accaatggct
     7381 atttttatgc ttacacctga ttatgatcca tctaaccata tcattgttag tatttttctt
     7441 agtcagttat ataaagaatt atctacgcaa tgtgttaaga ctaaaggaga taaatgtcac
     7501 cgcaggattc attttatttt agatgaattt gggaatatgc caccaatgga taatatggaa
     7561 ggaatcatga cggtcacagc tggccgaaac atgttgtggg atctgtttat tcaatcttat
     7621 cagcaacttt atgcgaagta tggagaacaa aacgcagcga ttatcaaaga aaattgtcag
     7681 acacatattt atattatgtc gacagatgat aataccatcg aagaattttc taaaaaagtg
     7741 gggaataaga cggttgaaca agagaacagt accatcaatt cattaggtat gaatactcat
     7801 atcaatcgca atgttgattc agatcgtatc ttaactcctg aaagaatttc tactttttta
     7861 gaaggagaaa cgattgtact cgctccttta aaacgacaag atttgaaagg tcataaaata
     7921 cggccgtatc ctatttttaa tcatggggaa acgaatcttc cttatgctta tcaatatatt
     7981 gaagatttca atccatcgtg ggatatcaat gatatagaca tacattgtac acatgcggac
     8041 ttggatctta aggctttaca aattgattat gattatttcc taccagatga atatgctaga
     8101 aatctctatc atggactgca tgatcaatta gaagaaaaaa acgaagaaaa tatgttttat
     8161 aaaatcgttc aatctcttga gaatgagacg gctaaaaaat tgttaattca attttacgaa
     8221 gaacaagcag cagatcgact tcaagaatat gttgaaaagt tgatcaaaaa taaagaaatt
     8281 tctgaaatga ttgggaaacg gttgttattg gaatctgaaa aggtggtgaa agaatgatca
     8341 ttggaggaat tttttctggc atagccgact ggtggtcagg cttattcgga gaaggggatg
     8401 caaaccccaa aaccgtaacg aaagtcttac aagattatgg gaattatctg cagtatcaag
     8461 atgtatttag ttggatttgg aacacttttc tgtggggatt gattaaagga ttatataatc
     8521 tcaatacttt actagaaaaa accatttatc aaagctttga tttaaaagac ttattgaacg
     8581 ccgctggggt aaatgcacta tttcaaagtt tgattagtaa ggtattggct cttttttgtg
     8641 tcgtcacatt aatttattta ggcttgaaat tttttatgag taaacaccca ccaaaagtca
     8701 aaaatatctt ggttaattta tttatggcca tgatcttgat ttttggtggt agttctttaa
     8761 tcgatgaagg gttaaatatc agtaaaagct tttacggaga cgtcactgca gcaaataaag
     8821 cagataaaac gggatcacct gcttttcaaa ttattcaaaa taatgtgatc gatgtcagta
     8881 cgttactagc aactgacccg aataaagtag aatccctacc aacagataaa agaaatgctt
     8941 taacagcgaa aaattttaaa acagcgaata tcaatgggat tattactcct gaacaagcta
     9001 aaggtatggc aggtaatgat aaaagtgggt tatcagatat acaaaaggat cggttaaagg
     9061 cattacaata ccgattggaa ctagatgata atgggaaaga aattcctgtt caaattagtg
     9121 acgaaggctt agcaaaatat gtctatactt cagggtatcg tcgatacagt agtaaaccag
     9181 gtattatttt agctggtgaa tcttctttgg cagtagccta tttatttatc ctatttacga
     9241 tcgttacttg cattattgag ctggtattta aaaaattcta tttagtgatt gccgcgtcaa
     9301 cggactttga gacaggtcaa cggatgaaaa gtgcgataga agatatttct caatccttct
     9361 tgttattggc gtttacgatt ttagaattac gaatctatac cttaatgctt tcaggtatcg
     9421 gtgatttgca tgcggcagga aagatcaatg gattcttgta tgtcgttgct ttgattgtac
     9481 taaccattgc gctattcaaa ggctctcaat cagttacgaa aattttcggc gttgatacaa
     9541 gcctgaaaaa cggcagtaat tctcttctta gcatgtttgc cttaggaaat attgcgaaaa
     9601 atgtgggtag cggagctaaa aaccttgcaa gtgctggtaa gagtggttta agccatttaa
     9661 atgatattcg taaaaatgga tttggccatc gaaaagatgt tacccctgat aacggggaag
     9721 aacaatcaga cgataaagct acaactgtac ctaatagtaa gaaaacagtt tccgaaatgt
     9781 tcgggaaagc gaaaaatggt gtttctaaag ccgcagaagg tcttggttac gtcaatgaac
     9841 gtgggttaaa aggaacagct gaagatctta cagaaaaagc caaagacacc ttagaagata
     9901 aattagacgg cacgagagct aaagaattgg ctgaagctgt gaagaatcca tccgaagcac
     9961 tagaaaaaca aaaagaaaaa gcttcagaag tcaaagaagg actaaaagat gcgtatggtg
    10021 aaggacaagt caatgcagca gtgaaagcta atacaacaac cgaaaaagaa aatcaagaag
    10081 gtatagtgat tccttcagtc aaagatcacc aagaagcgtt gcactctaat gaaaattcga
    10141 ttgaagagaa aaattccata gaagaaagaa attcagaaga aggaaaaaat ggacagttac
    10201 caaaaactga tccagaacaa tatcgctata aacctaacag tgaccttgaa aatgagaagc
    10261 gaatttcaga tttatcaaaa gtaaatcaag aagacaaagc agaaaatgag ggacagttac
    10321 caaaaactga tccagaacaa tatcggtata gatctgttaa ctccgaaaat gaaaaacgca
    10381 tttcagattt accaaaacga aatcaagaag tacaaacgga tgaggattcc atcgttctgc
    10441 caaatgataa tggaatcgaa gttggaactg aaaaatcaac gaaaattggt ttacctaagg
    10501 cacaaaattt accaatgaaa gatagtcgtt tgaatcaatt caaggataat gatcaaagtt
    10561 ttgttcatca atcacctgca ggtactgttc aaatgaaacc aagtcgtttg cctgaaaatg
    10621 gaaatctgac aactgtttct tacaatttat ctgaaagtgg tgagacaggg aatgcgtccg
    10681 ttcaaaatgt tgtgacaaga gaagctgcac caaaacctat tcaaaacgtt catacaggtt
    10741 acaagccagc aaatgtggca tttactgcac caacaaaaat aaatgtgaca agagaaacaa
    10801 aaaacaggaa caatgcattg aaagaacaac tattgaggga tatcaaacag gatatcaaac
    10861 cataaaaggc ctacaagtta ggcctttttt ctatgtatga aaaggagaaa tcatttatgg
    10921 ataaatatag taacatgaaa aaccctccaa gatttagtgg agaatggaag tttttcgctt
    10981 ttttcttttt agatctatta acgcttggtg gctgcttttt ttgggcgaaa atggtcaata
    11041 atcttgtcca cttaccactc ttttttgcac tcttgaatta tggcttatgg attgcgatag
    11101 cgttagtgat tgtttggcga ccctataata acccaggtca acgacaaatt tttgcgatta
    11161 tcgagtctat tttttttgcg gacaagaacc attatcatgc gttagatcct aaccgaaatt
    11221 atcatggtac gtataaggag gatgaataaa tgcgagtcaa aggacaaaat tcagttgaaa
    11281 ggaaatccat tttgcagttt ggaaaaccag aagagcgctc tatcttggac ttatttccat
    11341 ataaatcctg tacagaagag ggagtcttaa ttactagaga agatcatttt caacgttatt
    11401 atcgtgtagt ttctacagac gtggaaggat taaatgaaca agagaaacta gaacgaatga
    11461 atcagttgac caccgtcatg cgtacgtatg tgccaacaat taagttagtt agtttgacca
    11521 cagagacgga tctaacggaa caaatcgtgc aaaagcggca attattagat aaaaatcgta
    11581 tggaacagtc cttaggaaga aatctacaac gattaaaaaa atatgaaaag aaattagtcg
    11641 aagaaatcca ggaattgaaa cgagcagaac aagaacgtcc tgacttaagc ttctttttcg
    11701 tcattgaagg aaagacaatc aaggatttac aaaccagaaa ccgtcagtta atgcgatcta
    11761 gtggcatttt agggttaaaa ccactctaca aaaaagaatt gatcaagatt ctgtatcgaa
    11821 tgaacaatat gaatgatgag taggaatgga gaagagagaa tgaaatatcc gttgatatgg
    11881 ttagaaaaag atgttccgaa ggaagagacc actagaaaat ttcctcacac tttaagcctg
    11941 ggtaagttgg gaaaagggca atcttttaac caagtaaaga atcaaacaag aagaggtgac
    12001 tgtacatgtt agaagcaatt aaaacagctt tttcaccaca aaatgatcaa aaagtacttg
    12061 atccattaaa gcaactagga aatcgggaaa tagaaattgt aaaatccaaa gggtatgatt
    12121 tagatttttt aaaacaagtg atgccacaag gtgggttaaa ttttgatgaa gaatatgtga
    12181 cgtatggtaa tggttattca cgatgtgtga cagtttatca ctacacaaaa aatcctacgc
    12241 ttttttggtt ggccaatctc atgaacaatc ctcatacagt ggccacactc gatgttagca
    12301 cagatgaaaa gcaaaaagtg attgatcaaa tcaatcgtgc cttagatgaa ttaaatgatc
    12361 gatcagataa tgaacgaaaa gcgactgacc gaaataaaag cgcggaagaa tacctggaac
    12421 tagcggaata tgctagaaaa ttgacttcta atggggaaat ttctaagcaa gtcaaagtga
    12481 gagtacatgt ttacggaaac acgattcaag aagtagatga acgagcgcag gaactgatcg
    12541 aaaatctcaa agggatggat tatcgtgcgg tgacttatct gcataaaacc aaacgagatt
    12601 ggcaatcgat gtttaccatg ttcgatgaac aagctgaatg gttcggaaat atcaaagggc
    12661 aatcactacc atcgttaaac attggcggag gtttcccgtt ccaccaccaa acgctaaaag
    12721 atccaggagg gacacaatat ggggaaacca tgacaggcgg cccatttgtc tggaatgcga
    12781 cgtataaaaa tagtgtacgg ttgtctacta ataccttgct tctaggaatg atgggagctg
    12841 gtaaatcgac cgttcttaaa atgatcgcgg aagcccattt agctgcagga gactttattt
    12901 ggggctttga aaagggaaaa gactttattc ctttcttaaa ggaatataac ggtattatgg
    12961 tgcgtttaga cggatctgac ggaatgatca atcctttaga gatatttgcc acacggacgt
    13021 atgatgaagc atccagttta tatgacgatg gatcagtcaa agatttgaag atcaatgaag
    13081 cagcgtccta tcaaacacac ttagacaaag tggtctatca ggtacagtta gtgagtcctc
    13141 agctaaaagg aacgatgaaa gcagaattta agacctactt aaatcgtttt tatgaagagt
    13201 atggaacagt accacgcggc tttacttcct caaattcgcg ttcaaatact gatacgcaag
    13261 tcacagggaa agatccagaa gcttatccga cgttcaaaga atttttagac tttttaggac
    13321 aactggaatt accaggtgct tcccaagaga aaaagaatcg aaaagaagaa atggaatcca
    13381 tcgtagaatc cttatgtgag acgtatggca tgatttttga tggtcatagt acgattcgac
    13441 accttgacca acaacagttg gtcttttttg acattgactc tattacaggc ttagatcgag
    13501 gggtacaaca atgtcaaatg tacatggcta tgacgttgat ttggaatcag gcattgattc
    13561 aaggaagaag acaacgacgt ttaattaaag aaaaacaaat ggaagaacaa gacttcaaac
    13621 gcttcttggc gatgattgat gagtgccata atggtgcgac ctgttctgca gtgaaaagct
    13681 gtgggttgca agtagtctaa aaactattgt aataactctt ataacgagaa gtcaaatgag
    13741 tgggtaacgc cacaaagggc ttccactgat actccgacat gcaacaagta gtttatgcag
    13801 accaacgttg tgtgaagctc ggtgaagtcg gctgaaggat accgtaaggt aaaagaaata
    13861 gtgaatacca cgaaagttct ctagaggtaa agggtgtatc cgataagtcg ggtgtccaaa
    13921 aaaacttaat atggtgagta tgtacaaacg tactgacgaa ctagcgaatg tactgtccga
    13981 aaggcatagc tcctagaaat gggagagaca gtgagcaaaa aatcactcat tgtgctaact
    14041 tgtggttgag taaaagtcgt tgttatgaaa gaccatgctg cgttacaggc gtaggagaag
    14101 ttagggggca aaagctagca tctaagttaa ggcatgtgga tagatataag ggaacagggg
    14161 aagccatcaa catggagagt gcatactcga tgaagtggta gtaagaaata tgtgaatgac
    14221 aaataactta ctttagtgat ggtgaaagta gagtcacgac tgatgaaggt tctgtaatgg
    14281 aactggagga acaggctcga ttcgtctgta aaagtcatga aacccgttca accatgggat
    14341 tctcgtatga ctaaaaaggt cacgagccaa ttggagcgtg ataaaccatg acaaaagaaa
    14401 caagtccaaa agataggaag ttgcggcatg cagaatatta tggcatgacg gaaatcttcg
    14461 ataagatata ctccaaaagt ttgaagggag ataatttcaa gaatctaatg cagattatca
    14521 tttctgataa caacattgtc ctagcatatc gtaatatcaa gagaaatcac ggtagtgtga
    14581 caagaggaga agatggagta acgattaaag atattgaaaa tctcaaccgt aatgaattcg
    14641 tagaaattgt taggaagcga ttcgcacatt ataaccctcg gaaagtaagg cgtgtagaaa
    14701 ttcctaaata taatggtaaa ttaagaccgt tgggtattcc atcaatatgg gatagaatcg
    14761 cgcagcaatg catcttacaa gtattagaac caatatgtga agcaaaattc aataatcaca
    14821 gctatggttt ccgcccaaat agatcagcag aacacgcaat agcagattgt atggcccgaa
    14881 tgaatcaaag tcacatggaa tatgtggtag atgtagatat caaaggtttc ttcgacgaag
    14941 tcaatcatac taaactcatg agacaaatct ggtctatggg tatacgagac aaacagcttc
    15001 tcttaatcat taggaaaatg ttaagagcac ccgtagtact tccaaatggg aagttgatat
    15061 acccaacaaa aggaacacca caaggcggaa tacttagccc attacttgcg aacatcaatc
    15121 tcaacgaatt tgactggtgg atagcaaatc agtgggagga aaaagatgcc aaagagctaa
    15181 aacatcattt taggaaaaat ggagcttggg ataaaggtaa tatctatcac aagctcagaa
    15241 agagtacaac tctaaaagaa atatacctag tgagatacgc agatgatttt aaaattttct
    15301 gtagaaatta taatgaagcg aaaaaagttt tctatgcaac taaaatgtgg ttacaggagc
    15361 gtctaaagtt accaatatct gaagaaaagt caaaaatcac aaatctttca aaagaagaaa
    15421 gtgaattttt aggctttacg atgaaattag tgaccaaagg taacaaaaag gttgttaata
    15481 cgcatatagg tacaaaagca caaagcatga ttaaagtgaa actaaaaaaa caaatcaaac
    15541 gaattcaaaa aagtcctaat agcaaagata cctttatcta tattggagtc tataacagta
    15601 tggttattgg gattcataat tacttcaaaa ttgcgactca ttgtagtgta gcaatgcgaa
    15661 aaattgcaag agaactcaga ccaagttttg aaatacgact acgagaacaa ggaatctcaa
    15721 aagaaggaca atacggaaag tacgaaggat acaagcctta cctacggtca ggacaaatac
    15781 gatatataag agggtttcca atattaccag tagggtatat tcaacacaga catccgatgt
    15841 gtaaaaaaag aaatattaat aaatacaccg aagaaggtag ggaagccatt cataagaagc
    15901 aacaaaccgt agaagaatgg aagatatcgt ggttgtgtag caatccagtg cttagtaaga
    15961 gagcaacgat tgaatttgcg gacaatcgaa tttcaaaatt catcgctcaa aagggaagat
    16021 gtgcagtaac aggtgaggag cttatcttat ctgaaatgca ttgtcatcat attatccctt
    16081 atcatgaaag caaaagtgat agctatgaaa acctagtgat tgttactgag gaggttcatc
    16141 gggtaataca cgctacacaa agtgaaacga ttgaggaact tctaaaatat ctaaaattaa
    16201 atccaaaaca aaaagaaaaa ctcaatgagt tgagattaaa agttggtaac gaagaaatat
    16261 cttaataaat atttgtagga cgggaaaaca gaatgtcttt tatagacgag ggaacgccgt
    16321 atgcggtgaa agtcgcacgt acggtgtgga ctgggggaaa agacggagat aatatcaaac
    16381 tcttacctat cagtatcgat taatccagat tttattgaaa cggtaaatta tgtcacaaac
    16441 tttcaacgag aaatgcgtaa agtagaagca atgactgttt tagctacaca atccccacaa
    16501 gaaatggtgc cagaaaatat gtcttctgat aacttctcga aactgaagaa agtctttgaa
    16561 ttttcttcta cgaagatctt tatgcgtatg gatgaatcta ttttacgtca tatagaagga
    16621 ttagtgggta aatcaatgac gaactcagaa ttagaatcca ttcctcaaca aaatcaagga
    16681 gacgctgtga tcaattttgg ctcaaaagaa accattcgtg ttcattttag tccgaacaaa
    16741 cgtcaattaa aattgtttga tggaggaaaa taatggaaaa ccaaaatgaa agtttaatca
    16801 agcagtatgt taagcgtcgt gcaaagcggc gcttattttt gtggttgttt ggtacttctg
    16861 caggtttgat tactattttg atcactgtgt tcgtgaccct atttctaata cttgcggcgg
    16921 gctcgattga taattcggat tcagattcaa gcagtggtgg cgaagctttt acaggagaat
    16981 attcggaagg cttaccgatt tataaagaaa tcaaaggaag aggccctttt tctgatgaga
    17041 ttgcgcaata tgccgtaggt gcagcagtga aatataaatt attaccaagt gtgatccttt
    17101 cgcaatacgg ttacgaatca gcgtttggta catcggcgtc cgcaagaaat gatctcaact
    17161 attttgggat tacttggttt gatggttgtc tgtttcctaa aggaactgca cgaggaattg
    17221 gtggcattga aggtggctgg tacatgaagt ttcccaactc aaaagctgca ttttcctatt
    17281 acggctttat ggtagccaca caatccaatt ttaatgcttg tgtaggaaat aaaagccctg
    17341 gcgcaagctt attgatctta ggacgaggcg gttatgccgc tgcaggaatt acggaagata
    17401 gtccgtatta tacaggttgt atgtcaatta tcacttctaa taaattaacc gaatatgatg
    17461 aattcgccat caaacattgg ggtgaaggtg gcaataacaa cggcacgatt actggtgaat
    17521 ggacgaatcc attccctggc agcagtttag ataaaagttc cttcagcggt ggtcaattat
    17581 ttggaacgaa tccaggcggt gaattccgtc cgaacggctt ccatgatgga ttagattttg
    17641 gttcagttga tcatccagga agtgagattc atgcggttca tggcggaaaa gtcgtgtacg
    17701 ttggcaatcc tggtatttca ggtttgggcg cgtgtgtgat cgtgattaat tacgatggct
    17761 tgaatatggt gtatcaagaa tttgcaaata gtacaggaaa ttctcgagtg aaagtaggcg
    17821 atcaagtgaa agttggtcaa gtaattggga tacgagatac agcacacttg catttaggat
    17881 ttactcgaat ggattggcgt caagcacaag gacatgcatt cattgatgat ggtacatgga
    17941 tcgatccgtt accatttttg aatagcagta aaaaatagat caggagaaaa aatatggaaa
    18001 ataaaacgtt aaaaatcgtc ttaacagctg caattagcgt tagtttaatc ggcaatattg
    18061 cgttgttttg gcagctgaaa gaggaacaag cagcaaatac ctataaaatt gaagaagtgg
    18121 ctaaaaacgc gtctgagaag ctcaaaaaaa attcctctga ggcacaggcg aagatcgaca
    18181 agttaaatca aaccatcaat gagaaaaacc aagaaatcga tcagctgaag caacaagatg
    18241 gcacaaaagc aaaagaggaa gtaactgaag cacaaagaaa gacggcagaa gagttcggtc
    18301 aattggccat tgataaatca ttaagcagaa atgaattaac ggaaaaacta aaagatgtcg
    18361 caacccaaga agtgatcgac aaattaagtc ctacagacga tgatcaccaa catgatgatt
    18421 acggaagtta ctcctttaca ttaggtgacg tgcaaactta cgctcaaaca agcagtgtaa
    18481 aagaggaaca aaattttgtc gtatttgttg attatacaat cggcaatccg caattcaaag
    18541 atgtcaaacc acaaaaaata aaaggtggat taactgtcac tgaaaaacaa gtagatggtc
    18601 aatggaaagt catagacttt agttatttca ctcgttagga ggaagaaaat gagtaagaaa
    18661 aaaggagaaa gacaacgttt caaaaaacga aaaagttatc ttagatggct gggaatcttt
    18721 atgattctcg gctttttgat tcttgctagt ccgatttata cgaatcagaa ggtagatata
    18781 ccagatgagg taagcacgaa cgccttttta aacacgaaag agctgacaat ggtttcaaaa
    18841 gaaaaaggag cgaaccagca attagttttt gaatttgcga tcgatgatga agatcaaagc
    18901 ttgttaaaag aactggctaa tcttaattat cgcgtagaag taaagacagc taaaggggat
    18961 tatcaagcaa tacgagccaa agtgattaag gtgtctgatg attattttgc agtgaagcta
    19021 acgcatgtac cagaagaata tatcgccatg cgtttaacca ttattcctga aaaaattgat
    19081 ccaaaagtgg atatgcaaga accacaagat ctgatttatt acgtgcacga agataaggtt
    19141 aaagatcaag tgaaagatga agattatgaa caacacgcga tcaattacaa agtgaaagga
    19201 tacaagaaag aacaaaaagc agctcaaaaa caaatggaat ctttccaagc aaccattgat
    19261 ttgaatgaag aattagtgaa gaagctaaaa gatcaattgc cttatcaagt cgcagatgat
    19321 caggaaaata ctgaaaacaa gattaatagc taccagcaag aaattgaaac atcgaaaacg
    19381 caactgaaag atcaagaaga gatcattcaa aaacttcaag aaaaaattga tcgtatgaca
    19441 aaagaaaaga tttagaagga caggtggagt gaaaaaatga agcgaacaaa cttggctgac
    19501 aaagatactt tcgctgagca gaaagtattg attaaagcct atctcaaagt tgtaaaagct
    19561 aaagaaatat gtcgagaagt gaatttatct aacacaacaa tatctgagtt gaagaatggt
    19621 cgaaagaatt tagatacttt aaaattcaga tcatttgaag ctctatatgt agctgctaaa
    19681 aaatatttta cagctgataa caaataaaaa acaggagtat tttatgtgaa aaaagtatat
    19741 aaaaaatgct tatgaatcag ctatatcacc ttaaccattt gttaatgaga tgggagaaaa
    19801 cattaaccac gattctaacg caaacatcaa gcacataaaa catttaacac tcctcctttc
    19861 tggctgaaga tttcaaccaa acaggaagac agcgtatcaa ttatagcata cactttttct
    19921 taaattcatt tttcatatga caaaatattt ccttctaatc gacaaagtta gaaggatttt
    19981 ttattgtcta aatacacaaa taaccttaat ttttaaagag cataaggaaa cgtgacccca
    20041 cttttaaaaa aagtggttgg ataggctgtg cctatcacgt ttctgaaaaa aataaatgac
    20101 cccaaaatac caatatatag gatagtacaa ggatagcaaa ggatagtaaa aggatagagg
    20161 taggatagcg aaaaggatag gatccttttc actatcctat attatattgg aggatattta
    20221 tgaatgttaa aattagagat ttagatccaa aattcatttc tgaaattgat cgaagatgcg
    20281 ttgaactaag taatcgtaca ggaaataaat ggagtcgtaa tgattattta aagctactcg
    20341 ttgaaaatga ttttgatcgt cctttgatgg aatacaaaca agaaaagttt gaccagttat
    20401 tagaaaagtt ttcagaaatt caaagttata acacaaagat attagaagaa tatgttagcc
    20461 aaaataatcg tattatagaa atcttgattg agcaaaatag aggaagtgaa ctttgatatg
    20521 gcaaaggtta tagacaaaca catttacttt caccaaacac atatcgatgt attggatgaa
    20581 actatttcta ctcatgcaga ggttaaaaac tatagtgaag ctgttcgctt tttatgtatg
    20641 aactatgatc cagaagtgga gaaaaaagta aattccaatc aagcaaagtt gaatggaatg
    20701 agtaaagaaa tttctatttt aaccgaaatg gtttcagaat ttgcggatct tcatttgaaa
    20761 gatacaggaa tattaattgg taaaaattca actgtttatc aagaagcaaa agaacgagtg
    20821 gaaggaaaaa ttaaatccaa tcaaacaaaa aaatattccc ctcagttgaa gaaacctgtg
    20881 aatgaatctg ttcatgccag agtagatcca tttgatccaa cgaagattct aaaaagataa
    20941 aaatgaagga ggataataat gactcaatcg ccttcagtta ttctcacttg tcaatttact
    21001 ttgccgaata aaaagtcatt ttctacttat attgactata ttacaaggga acaggctctt
    21061 gaagaaaagg aacgacgtac gcccgaagaa gaacaggaac ttcaacgaat caagaatgct
    21121 ttagaggagt tttatattcc tgaaggagaa actttttcgg agagtaagac gggtaaagaa
    21181 ttcagtgaaa cgtcgaatga agctcaatcg ttgatggaac atggaatcaa ttttgacaag
    21241 ttagaggaac aagattttac caattatctt tcttatatga cacgtcacta tgcgttagaa
    21301 caaaagaaag aactgacgca aagtgaacaa aaagaatacc aacgcttgaa ccaagcaatg
    21361 aaaaaatatc aaacagaacc ccataaaaaa gcttcaggaa atcttcctgg agttttttct
    21421 atagcttcag acgaagtgaa aggaagtgat ctgaaggaga ttaagcaaat cttccaaaga
    21481 ggacaacaaa aaggctcaat catttatcaa gatgttgttt ctcatgataa ttcgtatctt
    21541 gaaaaaatag gattgtacga tcccaaaaca gatactttag atgaagaagg gttaaaagca
    21601 gctggaagaa aaatgatgga gacattattc gagaaagaac aattgaatga aacaggttac
    21661 tggatggcaa cgattcaccg taacactaaa catatccata ttcattttgc agttgttgaa
    21721 aaagaaaata cgcgtaaacc ttacacggag gttgaaaatg gtgtgagtta tatcgtaccg
    21781 aaaggtaaga gaagacaagc aacattagac gcaatgaaga ccaattatgt acatgagtta
    21841 gaacgttttt cttatgaaaa ggatcgaatg attggtatgg agaaacgaaa tttattgacg
    21901 cggaaaagtg atttgcgtaa caccttaaca aaagtggtaa aagagccagc gcgttacgat
    21961 cgacacgcga tgaacttatt aaatgaagta tataaaaccc tacctgacaa acgttcggaa
    22021 tggaattacg gggatgaaaa acgaaccaaa ctttcaccat acacaagaga gaaactcgat
    22081 gagttaacca aatatgtttt aacaaaagaa cctgaatatc aagaatatat acagatttca
    22141 aaagcattga aagaagaagc aaaaagtctt tatggggaaa gcaagagaga atcaaaggat
    22201 gcagagaaaa atgctctctt tgatttaaaa aagcgcacag gaaatgccat cctaacagag
    22261 ctgaagagac aggatagtca aataaaacca ttaatagaaa gtgttgatca gatgcaatct
    22321 atttacaatg aacgatcctt tcgaacgctt aaacaattgc caacgaatga atacaaatat
    22381 agttacgaag aattcatgaa gcgcaggaaa caattccaac aaagagttgt cacgcagcgg
    22441 agtttgcgtc aattaaatcg aacgattaat gcccatgaag aaaaatacca cgcaagtaaa
    22501 gcatacgaag agcaacaacg aagaattcaa atggaacaag aaagacagta aagacagtag
    22561 gaggaatagc aatgaaacaa tatttagatc aatggaaagt tatagaagga tcattaagag
    22621 aagaacgaat cgaacaactt cctgattgtt tagaaaaaga gcatttgttc cagataagag
    22681 aaatgttgag aaacgaacag tttgatccaa atcaattttt agtcgttgag tacccagcta
    22741 ctggtgtgta ttgttgtaat catgtgaatg gtgaaaaata ttttatcatt caagaatatg
    22801 agggcaagtt agcaccttat tatactactt gggaaatgaa tgaagaggga atcaataact
    22861 ttccatgtaa aagca
//
LOCUS       DACDRN010000041        21784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDRN010000041 DACDRN010000000
VERSION     DACDRN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.041"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            133..3660
                     /locus_tag="IWJ80_001804"
     CDS             133..3660
                     /locus_tag="IWJ80_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9461519.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IWJ80_001805"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IWJ80_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP9461520.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IWJ80_001806"
     CDS             7448..7789
                     /locus_tag="IWJ80_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9461521.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IWJ80_001807"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IWJ80_001807"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP9461522.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IWJ80_001808"
     CDS             9146..11566
                     /locus_tag="IWJ80_001808"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP9461523.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWJ80_001809"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWJ80_001809"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP9461524.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IWJ80_001810"
     CDS             13000..13737
                     /locus_tag="IWJ80_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9461525.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IWJ80_001811"
     CDS             13751..14566
                     /locus_tag="IWJ80_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9461526.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IWJ80_001812"
     CDS             14579..15226
                     /locus_tag="IWJ80_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9461527.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IWJ80_001813"
     CDS             15242..15898
                     /locus_tag="IWJ80_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9461528.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IWJ80_001814"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IWJ80_001814"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP9461529.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IWJ80_001815"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IWJ80_001815"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP9461530.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IWJ80_001816"
     CDS             18240..18758
                     /locus_tag="IWJ80_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9461531.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IWJ80_001817"
     CDS             18865..19365
                     /locus_tag="IWJ80_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9461532.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="IWJ80_001818"
     CDS             complement(19645..20565)
                     /locus_tag="IWJ80_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP9461533.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="IWJ80_001819"
     CDS             20730..21743
                     /locus_tag="IWJ80_001819"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP9461534.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
BASE COUNT         7300 a         3769 c         4658 g         6057 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg tgcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaat
//
LOCUS       DACDRN010000042        21774 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDRN010000042 DACDRN010000000
VERSION     DACDRN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.042"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="IWJ80_001820"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="IWJ80_001820"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP9461535.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="IWJ80_001821"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="IWJ80_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP9461536.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="IWJ80_001822"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="IWJ80_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP9461537.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="hflB"
                     /locus_tag="IWJ80_001823"
     CDS             complement(4246..6357)
                     /gene="hflB"
                     /locus_tag="IWJ80_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP9461538.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="IWJ80_001824"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="IWJ80_001824"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP9461539.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="IWJ80_001825"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="IWJ80_001825"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP9461540.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="IWJ80_001826"
     CDS             complement(8557..9027)
                     /locus_tag="IWJ80_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP9461541.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="IWJ80_001827"
     CDS             complement(9073..9498)
                     /locus_tag="IWJ80_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP9461542.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="IWJ80_001828"
     CDS             complement(9565..9834)
                     /locus_tag="IWJ80_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP9461543.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="IWJ80_001829"
     CDS             complement(9845..11440)
                     /locus_tag="IWJ80_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9461544.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="IWJ80_001830"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="IWJ80_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP9461545.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /locus_tag="IWJ80_001831"
     CDS             complement(14993..15553)
                     /locus_tag="IWJ80_001831"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP9461546.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15906..16880
                     /locus_tag="IWJ80_001832"
     CDS             15906..16880
                     /locus_tag="IWJ80_001832"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9461547.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17050..18450
                     /locus_tag="IWJ80_001833"
     CDS             17050..18450
                     /locus_tag="IWJ80_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP9461548.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18528..19250)
                     /locus_tag="IWJ80_001834"
     CDS             complement(18528..19250)
                     /locus_tag="IWJ80_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9461549.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19329..19835)
                     /locus_tag="IWJ80_001835"
     CDS             complement(19329..19835)
                     /locus_tag="IWJ80_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9461550.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19958..21757
                     /locus_tag="IWJ80_001836"
     CDS             19958..21757
                     /locus_tag="IWJ80_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP9461551.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         6038 a         4747 c         3903 g         7086 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttctcctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg ggatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta tacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa ataaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15781 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15841 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15901 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15961 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    16021 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    16081 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    16141 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16201 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16261 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16321 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16381 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16441 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16501 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16561 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16621 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16681 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16741 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16801 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16861 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16921 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16981 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    17041 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    17101 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    17161 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17221 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17281 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17341 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17401 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17461 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17521 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17581 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17641 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17701 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17761 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17821 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17881 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17941 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    18001 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    18061 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    18121 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18181 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18241 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18301 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18361 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18421 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18481 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18541 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18601 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18661 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18721 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18781 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18841 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18901 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18961 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    19021 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    19081 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    19141 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19201 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19261 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19321 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19381 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19441 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19501 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19561 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19621 agatggtcga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19681 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19741 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19801 tttccgtttt tcatttgttt ttttgaagaa ttcataagtc acctctgtat tttatcttag
    19861 actttttttg aagaaacaac aatcattttc atagagactt cttccataaa aaagtgctat
    19921 acttaatttt gtattacaat atgaagggag ttttttcatg agttattcat taaattggaa
    19981 tctagattct attttttccg gaggcagtca ttctgatgct ttgaaccaac ggatgaagca
    20041 attggaagac caaatgaacg agtattatca ccgcgtaaca aaatggtcac ctagttctga
    20101 caatgcagag cagttaaacg ctattcttca attgcaagaa acgattacga atggttttac
    20161 ccaatgcagc agctatatta ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa
    20221 ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg caatcagccg aaactgtatt
    20281 gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat cagatgcttt ctcaatcttc
    20341 gtttgaaaca attgcttttc gtttaaacga gattcgtcgt gatggctcac aattattatc
    20401 agaagctgaa gaaaacatta taaatacgct ttctttagat ggtctaaatg cttggagcag
    20461 tcattatgat acgattgtag ccagcatttc gattcccttc gaacaagatg gacaagtagt
    20521 cgaattatct gctggacaag ccttcaataa gatgatgggc gatccagatc caaaagtcag
    20581 agaaacactt ttcgcagcat gggaagaagc ctggaaagaa aaggcacccc tcttcacgga
    20641 tactttgaat catttagatg gttttagact ttctgactat gaacttcatg gtgtcacaga
    20701 ttttctacaa aaaccattag agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg
    20761 ggatacaatc cagaaaaaca aacaacccct tgttaattac ttgactagaa aagctaatct
    20821 tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca cctattattt taggcgattt
    20881 gaaagaaaaa acctttagtt ttgacgaagc agcagcattt attatcgaaa actttaacaa
    20941 attcagtcca aaaatggcta aatttgccca atctgctttt gaaaagagct ggatcgaagc
    21001 agaagatcgt cctgggaaac gtcctggtgg ttattgtact gagttaccag aaacaaaaga
    21061 atcaaggatc tttatgacct acagtaattc tgttaatgag gttgcgacac ttgctcatga
    21121 actaggacat gctttccata gcagcacgat gtgggattta ccttctttaa atcgcgaata
    21181 tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa ctgatcgtag ccgatgctac
    21241 attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa
    21301 tgctttagct atgttcatga atatccattc ccgattcatt tttgaaaatc gattctatga
    21361 agcacggcaa gaaggattag tctcagaaga taagatcaca gagatgatgg ttgaggcgca
    21421 aaaagaaggc tatcaaggag ctttagctac ttaccatcca tatttctggg ctgcaaaatt
    21481 gcatttcttt attgatgacg taccgtttta taacttccca tacacatttg gctacctctt
    21541 tagtttgggc atctatgctt acgccaacca aaagggagca aattttgaag aagaatatat
    21601 ccatttacta cgcgatactg cctcaatgac gacggaagat cttgcgaaga aacatttgaa
    21661 tgtcgatctt acccaaccag atttttggca agcaggtatc gacatggtga ctgaagatat
    21721 ccaaacgttc atggaattga cagaagaatt tatctgattt ctattttatc acaa
//
LOCUS       DACDRN010000043        19946 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDRN010000043 DACDRN010000000
VERSION     DACDRN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.043"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(89..1669)
                     /locus_tag="IWJ80_001837"
     CDS             complement(89..1669)
                     /locus_tag="IWJ80_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP9461552.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2070..3446)
                     /locus_tag="IWJ80_001838"
     CDS             complement(2070..3446)
                     /locus_tag="IWJ80_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9461553.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3574..4692)
                     /locus_tag="IWJ80_001839"
     CDS             complement(3574..4692)
                     /locus_tag="IWJ80_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9461554.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4834..5268
                     /locus_tag="IWJ80_001840"
     CDS             4834..5268
                     /locus_tag="IWJ80_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9461555.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5346..5879)
                     /locus_tag="IWJ80_001841"
     CDS             complement(5346..5879)
                     /locus_tag="IWJ80_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP9461556.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(5973..7151)
                     /gene="mutY"
                     /locus_tag="IWJ80_001842"
     CDS             complement(5973..7151)
                     /gene="mutY"
                     /locus_tag="IWJ80_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP9461557.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7144..7941)
                     /gene="recX"
                     /locus_tag="IWJ80_001843"
     CDS             complement(7144..7941)
                     /gene="recX"
                     /locus_tag="IWJ80_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP9461558.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8048..9430
                     /gene="rlmD"
                     /locus_tag="IWJ80_001844"
     CDS             8048..9430
                     /gene="rlmD"
                     /locus_tag="IWJ80_001844"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9461559.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9604..10182)
                     /locus_tag="IWJ80_001845"
     CDS             complement(9604..10182)
                     /locus_tag="IWJ80_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP9461560.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10504..10974
                     /locus_tag="IWJ80_001846"
     CDS             10504..10974
                     /locus_tag="IWJ80_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP9461561.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11082..12308)
                     /locus_tag="IWJ80_001847"
     CDS             complement(11082..12308)
                     /locus_tag="IWJ80_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP9461562.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13062..13262)
                     /locus_tag="IWJ80_001848"
     CDS             complement(13062..13262)
                     /locus_tag="IWJ80_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9461563.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13396..14004)
                     /locus_tag="IWJ80_001849"
     CDS             complement(13396..14004)
                     /locus_tag="IWJ80_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP9461564.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14089..14559)
                     /locus_tag="IWJ80_001850"
     CDS             complement(14089..14559)
                     /locus_tag="IWJ80_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP9461565.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(14607..15521)
                     /locus_tag="IWJ80_001851"
     CDS             complement(14607..15521)
                     /locus_tag="IWJ80_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9461566.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            15667..16470
                     /locus_tag="IWJ80_001852"
     CDS             15667..16470
                     /locus_tag="IWJ80_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP9461567.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            16648..17595
                     /locus_tag="IWJ80_001853"
     CDS             16648..17595
                     /locus_tag="IWJ80_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9461568.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(17704..19251)
                     /locus_tag="IWJ80_001854"
     CDS             complement(17704..19251)
                     /locus_tag="IWJ80_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP9461569.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(19353..19706)
                     /gene="tnpB"
                     /locus_tag="IWJ80_001855"
     CDS             complement(19353..19706)
                     /gene="tnpB"
                     /locus_tag="IWJ80_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP9461570.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(19696..19890)
                     /locus_tag="IWJ80_001856"
     CDS             complement(19696..19890)
                     /locus_tag="IWJ80_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461571.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT         5983 a         4020 c         3318 g         6625 t
ORIGIN      
        1 ccgtcacggc tctaaacaaa atatgagagg ctgtttttgt ttggctattc ttattacttg
       61 atgcagggca gcttgtgtca agacttcttt acatcaaact ttttaatttt acattgggat
      121 atttatccgc aaaccagcgt tctgcaaatt ggttctcgaa taagaacaac ggttggtcga
      181 aacggtcacg agccaagata ttgcgacttg aactcatctt ctcatccaaa tcttctggat
      241 cgatccagcg agcgattttg tgtcccattg gagacatgat tacttcggcg ttatactcat
      301 ttaacatacg atattggaat acttcgaatt gtagctgacc aacagctcca atgatatatt
      361 cttccgttag atatgtttta taaagctgaa tcgctccttc ttgaactaat tgatagattc
      421 ctttgtggaa ggatttttgt ttcatgacat tttttgcagt cactttcata aacagctctg
      481 gagtaaatga agggagttct tcataagcaa cttttagttt tccttcgaac aatgtatccc
      541 caatctgata attgcctgta tcatatacac cgatgatatc gcctgctact gctttttcga
      601 cgttttctct cgaatctgcc ataaattgtg tgacattgct taatttcagt tttttatttg
      661 tacgttccaa ccagacatcc attcctcgtt cgaatgttcc agaacagatt cggacaaaag
      721 cgattcggtc acgatgggct ggattcatat ttgcttggat cttgaatacg aaacctgaaa
      781 attcttcctc ataaggactt accatctgat catcttgtgt tttgtgtcca taaggagctg
      841 gtgccaattc aacgaaagtc tctaaaaaag tctgtacacc gaagttagtc aatgcagaac
      901 caaaaaatac tggtgtctga tcgccacgag caattttttc ttgattaaat gcatctccag
      961 cctctttgat tagttctact tcttctaaga cttgttcata taaggaattt tcagttagag
     1021 gatgattttc gggaagcagt ccatcttcat ccaattctac taatcgttca tcctgataat
     1081 tttccggacg ataaaactct acacgattat ggtagatatc ataaagacct tctagccctt
     1141 tacccatacc gattggccaa ttcatcggat aagattcgat atctaatagc tcttctaatt
     1201 cttctaacaa ttccaatggt tcacgaccat cacgatctag tttgttgata aaagtaaata
     1261 tcgggatgcc acgttttttt acaacttgga ataatttctt tgtctgtgct tcgatccctt
     1321 ttgcactatc gattaccatg accgcactat ccacggccat caaagtacga taagtatctt
     1381 cagaaaaatc ttcatgtcct ggtgtatcca agatatttac tcgtttccct tgataatcaa
     1441 actgcataac agaactagta acagaaatcc cacgttgttt ctcgatttcc atccagtctg
     1501 atttggcaaa attacctgtt tttttgcctt ttacggtccc ggcttgacgg atagccccac
     1561 caaacaatag taattgttct gtaatcgttg ttttcccagc atccggatgg gaaatgatcg
     1621 caaaagttct gcgattgtcc acttgttgct ttaattttgg attattcata acctaactcc
     1681 atttcttcca ttcaattatt tatattttct ctaggcacag attgtccgat ctcttaaaga
     1741 aagacaattt catatagggc tgtgacaaaa gccgtgtagc agtctctttt tccgagtaaa
     1801 cggcgttcta gagacagcct atcagcaata agcccaccca agccaaaaat acgaaaagct
     1861 gtttttgctt ggctgctctt attacttaat gcaggatagc tttttcacaa cctttgtcaa
     1921 ctactacaac tgaatcagta tagcatggat tcagcgaatg aaaaagccac caaaccataa
     1981 gagtcaaata tttttttgtc agctgactaa aagaaaagac gtgttgcttg tttattcaag
     2041 caacacgtct ttcataacca agtttccaat tatcttcttg gctcgtcatc ttcgaattct
     2101 tttctgaagt atcttcgttc ttcttcaggt tccgcttctg tttcttccgg atcatgaaaa
     2161 atgacacgga ttttaaggat tcgagaacct tccatttctt cagaaaccag cgtaatatta
     2221 tcgacatcaa acgataactt ttcgccttca tccggaatca tgcctaatgc tgtaatcaaa
     2281 taaccagcca tcgtatctac atcactcata tgcagatcac tgtcaaaagc ttcattgaac
     2341 tcatcaatca gcatacgtcc ttggatgata tattcgtgtt catcgatttt ttcataaaga
     2401 ttctcaactt catctgattc atcatcgatt tctccgacga tttcttctaa aaggtcttct
     2461 agtgttgcta acccaacgac gccgccatat tcatctaata aaatagccat ctgattctgc
     2521 gtctttttca tttcatataa cagatcatca ataaagaccg tttcaggaac gaataaaggc
     2581 tcctgcatga ttttcttgat atctaaatta tcaaagccga atttatgggc agcttttagt
     2641 aaattctttg tatgaaggat tccgactact ttatctttgt cttcgttata aacagggatt
     2701 cttgaatagt tttcggataa aacttcattc acattttctt ccacagtgtc gttgatatca
     2761 atcataaatg catccgttcg tgggaccatg acttctcggg caactttcgt atcaagagaa
     2821 aaaacccctt ggagcatttc taattcttca ttattcaata cgccttcgtt ttcaagcata
     2881 taacgcattt catctctggt catttttgaa tcttcatcat caaaagtcat cggcgtgata
     2941 cgcgccaata aacttgtgga agcagaaagc agccagacga agggcttagc tactacacca
     3001 atcaagcgta tcagccctga agtgaacttt gcgacttctt ctgatttatt taaagcaata
     3061 cgtttagggt acaattctcc aaaaacaatc gatacatagg tcaaaatagc cagcacaatg
     3121 atatttgcaa tattttttgc cgctgtgccg ccacctaata ccggagctaa ccgttccgat
     3181 aaagtattag ctagtgaagc accagacaaa atgttgacca aagtgattcc tacttggatt
     3241 gtcgatagga aattatttgg attctgcaat atttttaaaa gcttttgaga tttcacatct
     3301 ccttcatcag ctttttgctc gattcgattc ttattgatcg aaacaacagc aatttcggaa
     3361 gctgctaaaa aagcattgat caatgtcaaa acgatcaata ataaaatctg cgctaacagc
     3421 gactgactct cagggtcagc attcataatc gcattctctc cttataaaat taatttttag
     3481 tttacgatag aaaaaaacgt ggcactatag ccacgttaaa atggtatcac aaagcaattt
     3541 tctttgcaat aaaaacaagt gtactaaaac tacctacgag gtcgtagcaa ttttcttctt
     3601 gttttcattt tgcttgtctt ctttgacgat tttgattata taggaaatga tcgtcttgca
     3661 gattgcgtaa aaaggaacac ctaagaagat accaagcaat ccggcaatat ttccagctac
     3721 tagaagaacg ataatgatcg ttaatggatg gattttcagt gatttcccaa ttacattagg
     3781 ataaatgata ttcccatcca actgttggac gattagcaca accacacaac aaagtaatgc
     3841 ttttaccgga tcattgaaga ctgttactag aaatgcaggt gctagtccta gataaggtcc
     3901 taaatatgga attaggtttg tcaatcccgc aatgacccca aataagaaag cataatccac
     3961 accaattacc atatatccta aaaatgtgaa ggtaccaacg aataaacatt cgattgcttg
     4021 tccgctaatg taattagcca atgttttatt cagttgcccc aataactcca caatttggtt
     4081 tcttcttttt tctgggaaaa actgttggat gtttggcact agtttttctc cgtctttcaa
     4141 catatagaac aagatgaatg gtgctgtaat gattactacg gttgccgaag cgatcgttcc
     4201 aacgatcgaa cctaaactat tggatactcc actaaggaat tgctgaatga ttgtcccgta
     4261 agaaatgtct aattgctcaa aatatttatt gatatctact tccttgaaca aaggcaattc
     4321 ggctacctct tttaaccagc tttctacctg tttgatgaaa tcaggcatat tagatgccaa
     4381 tgaggaaatt tgctgtacta gacttgggat gacactaagt aaaatccaaa taattgctgc
     4441 aactaaaagg agcagcacaa ttgcgacagc ccatattctt ttgatttttg ctttcatcaa
     4501 cagcttgaca ataggattca ataaatagta aagaaagcct gcaatcagta caggtgcaaa
     4561 taacgtggaa aaaaatgtcc cgatcggttc gaaaataaag tttatttctg atgaaatcat
     4621 gatcaatgca gctatgatca ataattcaag cgaccaaaac attagtttag actgtctaag
     4681 tttttcaaac aatagatttc ctcctttctt tcttactagt atatattata ttcgttaaaa
     4741 attcttttat tcttttttta tttttgaaaa cttgccatac tttttatttg ttttattttc
     4801 cagtatacta tacgaaagga aggtggctag accatgaaag ttgtacaaac aaaagataca
     4861 atgagcaata tttacttaga tgctgtacgg atcaggcatc aagtttttgt agtcgaacag
     4921 ggtgtgcctt tgtctcgaga aatagacaaa gatgaagccc attgtatcca cttcgtcctg
     4981 tactcagata aaaaagaacc gcaaggaact gtacgtcttt tgcctttaga aaacggaaaa
     5041 atgaaattac agcggatggc gattctttct gaatacagac accaagggtt aggaaaaata
     5101 ttgattgaag aagcagaaaa ctttgctaag aatcaaggct ataatacgat tttattaggg
     5161 gcacagtcca cagctgaaac tttttacgaa aaactaggat atacagctta cggcgatcct
     5221 tttgaagatg ctggcatgcc tcatatttat atgaaaaaga ctttataaaa atttatttcg
     5281 aaaaaagcaa aaaacagcaa gcttttgaga aaaatctctc actggcttac tgttcttttt
     5341 tgtgtttact tacgcgacaa ttgcttgtaa cggttatacc aaatatcaat atagccttcc
     5401 gagaacgggc catctccatt attgatccag tcaaccagaa tcttcacatt ttcttttagg
     5461 atgaagtcaa tatcgttcgg gtaattcatc attttgctgt gcatctcata ttcatcgaca
     5521 tccaatagtc gtttctcgcc gtcaggaaat accttgatat ccaagtcata gtcgatatat
     5581 ttcaaggcct catcgtccag caaaaaaggt gaagctagat tacaataata ggaaaccccc
     5641 ttttctctga tcattgctat tacattgaac cagtattttt tatgaaaata aacaatcgct
     5701 ggttcgcgcg ttacccatcg gcgtccatca gattcagtga ctaatgtgtg atcattcacg
     5761 ccgatcatag agtgttcgct tgtttttaat accatggtat ctcgccacgt tcgatgcaat
     5821 ttgccgtcgt gcttgtaact ttggatcgtc acaaactctc cttcttttgg aactcccata
     5881 gcaacccttc cttaagcagc attttgtgct ttgtgtttgg gtgttcatta caccgacatc
     5941 ataattatta tatcaataaa aatgagcttt gcctagaaag gattcctatt gtttcgattt
     6001 ttctctaact gatctaccag tttcatttgg accttaggaa aaacgaccga atcaaatgca
     6061 tcaggcttca accacttcga agttttgttc tcggtaaatt cttgttctgc tccctcggtt
     6121 gcccgtccgt aaaaaaggag aacatgccat ttcagatgac taaaaatatg agtaacttct
     6181 cctaaatgtc gtgtctgcca aacaaagagt tcctgctttt caaatgggag attttgctct
     6241 tcttctgcta ctaggtcatc aaacagatcg atttctggct ttgtttccca ttcttttttc
     6301 aatcgctcat actcctcttg tgtcacttcc atcattggaa atgtccacat attcgccagc
     6361 aatttttggc tatctctttc ttcaaaataa tacgctccag aatgattttg aagagctgca
     6421 ctgatataat aaacgtcttt tggctttgct ttttttgttt ttacaggaaa actcgtttgg
     6481 attcctcgtt tgttagctaa acaaaaggct tggatcggac aagtctcaca ctttggagaa
     6541 gttggtgtac agatagctga acctagatcc atcattgctt ggttgaattc acctggatgc
     6601 ttttcatcaa tgattttccg catcgcttca tcaaagattt tcctgctaga agcttttgcg
     6661 atgtctgctt caatgcaaaa caatctgctg actactcgca tcacgttgcc gtcaactgca
     6721 ggttctggaa gtccaaatgc gatacttgca atcgcgcctg tcgtatatgg tcctattccc
     6781 ttcaatgagc taatttcttc aggcgtttgc ggcatttccc catcaaattc tgacatgatc
     6841 tgtttagcag cagcttggat gttccgggct cttgagtaat aaccaaggcc ctcccatgct
     6901 tttaaaagct tttcttctgg agcatttgct agctcttcaa ttgtaggaaa ccattccatg
     6961 aaacgataaa aataatcgat cactgtgtct actctggttt gctgaagcat gatctcagag
     7021 atccagatac gataaggatc acgattatag cgccaaggca ggtttctttt ttcttgttca
     7081 taccattgaa tgaactgatc ttgaaattct tttgtttctt tatctgtcca tttttcccat
     7141 tctttattca ctttcctctt ccttttccgt aatgaatcgc tggatcatat ccaaatcaaa
     7201 tcctttttga tataagcttt gctttacttt tagatttctt tttttaggtt caaaccttaa
     7261 atttcttcgc catagtttat ctccttgttt gactaaataa tcatattcta tttcttgatc
     7321 tgcttcttgc ggaagatcag ctatcgcttg ttggatcact tcttgagaaa agcctttcgt
     7381 catcaatgtt tgttggattt ttctcaataa ttctttttgg ctttttccgt gattttttcc
     7441 aaacgctttt tcggctgtct gatgtgcatt ttgaacttgg tcttcaaatg tatattggct
     7501 gagcgcttgt tcaatgattt caggtttcaa acctttttgc tgcatctgct gcgctaattt
     7561 ctttggtcct ttgtcactta agcgcatatt cgtgcgcaca taactttccc catagaccaa
     7621 atcatcgaca aggtccaatt cttttaagcg ggctacgatt ttatttcgat cttcttgaag
     7681 gatttccttt tcttttaagt atgttcggat ttctttttcc gatcgcaact gataacttaa
     7741 gtaattcatt gctaggtgaa aaccgaaatc agctccgcta ctcttcttga tctcttggat
     7801 agtttcttcg tctaattccc gtccttttaa taatcgatag cgtactaata catcttcaga
     7861 tacttgtaaa gcttctcctt cagataaatc tacgcgatag aaagggcctt tgcctttact
     7921 gattctcgta acggttaaca taaacatcct cctcgaacat atattctcta tcataccaca
     7981 gaaaatcccc gtaatcaggt attatatgtt agaataaacc acagaaaaaa gaaaaaggga
     8041 gatacttatg gcagaaacaa ttgtcaaaac tggacaaacg gttcctttga aaatcaagcg
     8101 tttaggcata aatggtgaag gaatcggtta tttcaaacgg ttgatcatat ttgttccata
     8161 tgcactacca aaagaagaag tactagtgaa aatcacgaaa gcaactcctc gctatgcaga
     8221 aggagaatta ataaaagtca aaaaaacaag caaagatcgc gtagtggcac catgtcctgt
     8281 ttactatgaa tgcggtggct gtcaattaca acacttagct tatcaagctc aattggattt
     8341 taaaaaggat ctgcttttgc aagcattgga aaaattcaaa ccagcaggct ttcgatctta
     8401 tcaacttttg ccgacgatcg ggatggatga gccttggcat tatagaaaca aagcacaatt
     8461 tcagttacga aaaaataagc agactcaaaa aattgaagct ggtttatacg aagcaaactc
     8521 tcatcagtta gtccctataa cagactgctt ggttcaagaa cctcgaacac aaaaagtaat
     8581 gaatactgtt gttcaactat tgaataaatt tgatgcaccc atctatgatg aacggacaaa
     8641 cagtggaatc ttccgcacaa tcatggttag aatcggtgtt aaaactgggg agttacaagt
     8701 tgtttttatt actcgcagta aaaaattccc gcaaaaaaac gcaatgatcc gtgagatcaa
     8761 tgaacgactg cccgaagttg tttcaatcat gcaaaatgtc caggcacaaa aaacgtctct
     8821 cgtgatgggg gatgaaacga tccacttatg gggaaaagaa gccattgaag aacaaatcaa
     8881 tgaagtgact ttcgatcttt ctcctcgtgc cttctttcaa ctcaatcctc agcagaccga
     8941 attgctttat ggcgaaggaa tcaaagcatt agatgtccag cctaatgaaa cgatcgtgga
     9001 tgcttattgc ggagttggga cgatcggctt aagtgtagca aaacaagcca aagaagtacg
     9061 gggaatggat accgttcctc aagcaatcgc tgatgcaaaa aaaaatgcgg aaagattagg
     9121 atatcacaac actcgatatg aagtcgggac agcagagcaa gttctgccta aatggttgaa
     9181 tgaaggtttt cagcctgatg gtatcatcgt agatccacca cgtacgggtt tggataacgc
     9241 attgatccaa tcattattga aataccctcc aaaaaaactt gtttatatct cctgtaatgt
     9301 ttctacactt gccagagatc tggttaattt aagtaaaaag ttcaagattg aatatttaca
     9361 gtccgtcgac atgtttcctc agactgcacg ttgtgaagtt gtcgtgaaga tgacgagact
     9421 cgctgactaa atgaaagagg ttgtgaaaaa gtctgccctg tatcaagtaa taagaacagc
     9481 caaacaaaaa tagctcttca tgttttatga atactgtact tgtgaccgaa gagttaacag
     9541 gaggacacta tttatttaaa aaaagggttg tgacatagtt tttgtcacag ccctttttga
     9601 ttattattgg atattcacgt ctatcacttg ataaaatgca tttgtcgtat cgctgatatt
     9661 ccatactgct aaaatcacat tatatccttt tcgatcctgc ggaagtgtga tcgtttggtt
     9721 gactgttttt ggtggaacag ttgctttgtc gtctattttt gtgatcaatt caaaatcact
     9781 aaatttcaaa ggttgattag gattccaagt tggtttggta atgtagtaat cccaagtact
     9841 tgtccgatgt tgtgctgtta aattccaagt aatatttaat gttcctggat taaccatgtt
     9901 tttatgccat ctgttagatg tctgttcatc taaaggctca aagccgctga ctcctgcact
     9961 agcaattttt ccatcaatga atgtattttt ggtagcttcg atactttgcg gttcccattg
    10021 tgctcttccg acattttgat ttaagtttcc tgctgctgat gttcctaggt atgcacggct
    10081 tcctggagaa gtaatataac catgcgcaaa acctggaatg ctaaaaccga acaaccctaa
    10141 actaactaac ccaataaaca ataacgtact aatctttttc atgtagaaac ctccttcatt
    10201 tgataaccac attgtaaacg ctttattgta ttaacacaaa cattatgctt ttataatttt
    10261 atgttgaagt aaaaaaggtg aaaaaagtaa gatgcagatt ataacagaag tagacgagaa
    10321 aagtacaaat agaaaacatg agaatcaaat catatgatct ttttcaaaaa catcattcat
    10381 ataaacaaat gttcaacttt ttaataacaa caattatcaa tttttttctt acttcacaaa
    10441 ctaaatacag gtcttgctgt gagccttcat ggtaaactaa tattatagaa aggagttagt
    10501 aaaatgaaga tgataaaaga tattcgtttt tgggtttgtg ttataggaat tgtcatatta
    10561 ggctttttat cagggttgct tagcggaaat cccggaaaat attactattc tttgcaactt
    10621 ccaccatttg caccacctag ctggatattt gggccgatgt ggaccttact gtatatattg
    10681 atgggtattt ctctttattt gttacttaat cacaacaaca aaaaacagcg aaacaatctt
    10741 gtcggtcttt ttgtgattca gttcattttt aattttatat ggtctgctct attttttaat
    10801 ttaagaaata ttttcattgc agcaatcgat attacacttt tagttatttt cttgtcagtg
    10861 cttatgtatc agttgtggct gcatcatcgc ctggcaatgt ggctcatgat tccttattat
    10921 ttatgggtac tattcgcaac tctattgaat tattcaattt attttttaaa ctaagcgcta
    10981 aaaagagacg tcctgtaatt taacacatag aaaaagtgcc acctaagtta gattttttgt
    11041 ctaatttagg tggcactctt tattaaccaa actatttttt tctagtaccc acttgttgtt
    11101 ccatactcat tattctggtc ataaccataa ccattttgtt cctgtccagc gtaaggatca
    11161 taactttgag tatcttgact tggtgtatag tactgacttg tatcttctgt ttgctgataa
    11221 taggtattcg gatcttggta ctgatcatat gtgttttctg aataggaaga ataatcggtg
    11281 tacccttcac tattcgatcc atcaccgttt gtcgaatcgc catcttccga atcatcaaag
    11341 aacatcattg aagcattctc atttttcaaa ttaggtaatg tatcgcttgt ttttatattt
    11401 aattgtttct tcagttcatt ttggatcgaa agaagatcat tttttcctaa aatttgatag
    11461 tagatatcat tgatcatttg tccttcgcct tgcaactggt cttgtttgat tgtttcaaac
    11521 gccggggtgt agttcgtcgc tacatcaagc atgtcatccc aatctaagtc cgtcgtcaca
    11581 ttgttctcaa ctgcttttaa aatttttcta taattgctta cgccatctaa acttaaaact
    11641 ttattgacga ttttcgtcac tacttcccgc tgacgacgtt gtcgaccgat atcgccttct
    11701 ggatcttcgt gtcgcattcg agaataggct aaaccttttt ctccattcaa tgtttgttca
    11761 cctactggta catggacacc atccaaagta aagtcaattt tattattcac tttgatgcca
    11821 ccgaccgcat tgatcaaatc ttccatgcca tccaaattga tcgttacata atgatcaata
    11881 ggaatatcga gcagttgctc aattgaatcc atggccattt cgacaccacc aaatgcgtag
    11941 gcatgattta atttatctac cgttccataa cctacgatat tcgtataaat atctcggtcc
    12001 aaactgatga tcgttgattt tttttgtttc ggattaactg ttactaccat catcgtatca
    12061 gaacgtcctt gttccgttct tcctaatcca ccggtatcta aacctaatag caaaaccgaa
    12121 aaaggttctt tgtcatcaat gcttactctc tcagaacgtt tggaggatat tcgattagag
    12181 tttttagaaa ttttgtttac tgtttggctc gcttctccca taaatctgat tccgtatgct
    12241 gataatccgg caatcagcaa aacgaatacc cctaacaagg ttaatagaac tttttgccat
    12301 cgcttcataa tacacctctg tttcacattt tctaaaatat tgtctcatat aactgttcaa
    12361 tcagcaaatt cactttttaa tcataagaaa actttaaaaa acatttttgt gtaacgatta
    12421 ttatttttta aaatttctca aaattcttaa ctatcatacc ataccgtgaa aaagatcgaa
    12481 agaccgaata cacgcagttc gcaatcgttt tatctttttt tatgtatttt ttccagaaaa
    12541 agcgaacaac aaggtatttt ccaaataatc gtgcagtaaa atgatcgcac atacctttcc
    12601 catcttttta acagtctttg cctaccgttc agattatcat tcgatttcac gacaatttct
    12661 tcctatgact ggtccttttg ttaaacccat cttctattcg ttttatttcc agctcactgc
    12721 atggctctta aaatgactcg aactagtgat tgcattacca tcatctttta atggatagat
    12781 atgctcctct tcaaagtaaa acgattttct tttaaagtaa tagaattgac atggtaatga
    12841 gtatgtatac tagtggtttc acaaatggtc ttttattcaa catccaaaaa ctgaaatttc
    12901 tttaaaatcc attactaacg aagaaaatgt tccttgcttg ataattagtt aggacatgtc
    12961 aagtgaacaa atgcatttac ttagccaaaa aatctaaaaa cttcataaat agacaaaaag
    13021 acacgactct ctaagaagtc gtgtctttat tcaacaaatt attataattt agtcacttca
    13081 gcagcttgag gtccgcgtgc accttcaaca atcgtgaatt ctacttcttg accttcttct
    13141 aggctcttga agccgtctcc ttgaattgct gagaaatgaa caaatacgtc atctccgccg
    13201 tctactgtaa taaacccaaa tcctttttca ttgttgaacc atttaaccgt tccgtgttcc
    13261 ataaatgtat atcctccaaa aaagttcttg caagtaacct tgcaagaaca ttatacccaa
    13321 tttagaaaac gctagcaagc tttcgctctt ttattttcca aattattttt aagactgata
    13381 tgacggagga aatggttatt ttgctgcttc gaaacgtttg tttacttcat cccaattaat
    13441 aacattccag aatgctgcaa tataatctgg tcggacattt ttatatttca aataataagc
    13501 atgttcccaa acgtccaaac ctagaattgg tgtctttcct tccatcaatg gagaatcttg
    13561 atttgcagta gaggtaatag ctaattttcc attttccatt acaagccaag cccatccaga
    13621 accaaatcgt ccagcggctg cttttttgaa ttcttcttta aaagaagaaa aatcaccaaa
    13681 agcttcatta atcgcttctt ttatttctcc tgtaggttcg ccaccagcat ttggtgccat
    13741 gatttcccag aaaaatgaat ggttagcatg tccgccacca ttgttacgta cagctgtctt
    13801 gatatctgtt ggaatagcgt ccatatcaga taataattct tctattgttt tttcgcctaa
    13861 ttctgggtat ttctcaattg ctgaatttaa attcgtcaca taagtattgt gatgcttatc
    13921 atgatgcaga tgcatcgttt cttcgtcaat ataaggttcc aatgcgtcat atgcataagg
    13981 tagatctggt aatgtgtaag tcataaaaaa attcctcctc aatgttttct taataatgtc
    14041 tacattacaa agagtaacaa agagaaggaa attcttcaat tgttatgcct agtcgtcttt
    14101 ttcactttga cctacttctt cttcaaaaac ctcagtttct ttaaaaataa acaacttgct
    14161 gaaaatatag ttcgcaagga taacaataat ttgagtgatg attttcgcca catactcatt
    14221 gctgttcata acattgatca acaataccat tgcccccatg tccaatccgt aagaaaggat
    14281 acggtagaag aagaacttac cgcattcaat aagtgcatgt gtaaaattcg gtgattttga
    14341 atggaatacc caaagtttgt tcgttacaaa agcaaacaat acagaacaaa accacgaaat
    14401 agaattggat aaggccatac ttaaatcaaa atattggcgt gcgagagcaa aactaacaaa
    14461 atttacaatc gtagccaaac caccaaaaaa gagatagact agtatctccc aatatccttt
    14521 tgcttctaaa tatcctttta actgtcgata ctgcttcatg agccaaccta ccttattaaa
    14581 aattcttatt tatcatactc tatatttcaa taactttcat ctttatttaa aaaaattaat
    14641 tttttttgat cctttgcctt tgtctctgct tggatcgtca catgtgtaat accatatttt
    14701 aattttaata aagaaattat acgaggatat atcttttcaa tctcgcttag tagctgatct
    14761 tcaaaatcaa tatgtccgtc gaaaataatt atcttttcgc tatactgcca aatatggaca
    14821 tgatgaacat cctttacccc atcgattgct tcaatttctt gttccatttt ttcatagtcg
    14881 atagcaggtg cactttgtat caaaatagcg gcagcttttt tgatcaccat tatcgcttct
    14941 atcaatatat atatcgaaat gatcagtgta ataactggat cgatccagta gatatcgaaa
    15001 aaacggatca aaattgctcc gataatcaca cttaccgaag acagtgtatc acttaacaaa
    15061 tgcaaataac tagattttat attcaaactt tcatctgctt cttgtcgtaa taataatact
    15121 gagaacaaat tcgcgagcaa tccaataact gctactgtta gcatcaaatg actatttatc
    15181 ttttctggtg aagtgaaccg cttgaatgct tcaacggtta aaaacaagga taatatgatc
    15241 aatatgaatg agttgatgaa agcactcaat atttctaaac gctggtagcc atacgtacgt
    15301 tttttatttg cttcttttcg ggccattttt tgtgccccgt aagaaaaaat aattgccaat
    15361 gtatcactaa ggttatggaa agcatctgaa acaagagaca aactccctga cataatccct
    15421 ccaataaatt ctgataaagt gatcaccgca ttcaaaacca ctactccaaa tagttttttt
    15481 gatgatctct gtggctgtgt aggcactatt ccttcctcca ttttatttat attggatagt
    15541 ctttagacaa gaataacatt tttttgatat ttcaccaatc attcagctgt ttccttaaag
    15601 cacttgtatt tcacttgata cttcgctaga attgcaagtg atgacactac tagatgagga
    15661 gtacttatga caacaaaaca attaattatc agttccttta tacgattcaa agacctaaag
    15721 gaggcgagaa atgtcccatt ttggaaagcc attgtctatc ttttagtttt gagtataatt
    15781 atggctttgc caatctcttt tcaagttttt caagtcttaa acaacattca attggatggg
    15841 caaaagattg ctactcgcat tcctgatttt acaatccaaa acgggcagct tcaaaccgaa
    15901 gaaaaagaag gattcattta ccaaacaaac tccattatct tcacatttga tccagaggga
    15961 aaacgaacag aacaggacat ctctactgat ttgatgggga attttgttag tgtcgggatg
    16021 ttaaaaaaca aacttatcat agctcttcct aatacaggca cgacttctgc tttgttgaac
    16081 aataaccaat tagaacttcc ctacaccaat gaatctttaa aaaacttaac aggtaagcag
    16141 ctccgttctt ttttaagtga agcatctata ccactttgga taaaagtcct gacatttata
    16201 ttttctattt atcctagttt tctgaatctg ctgatcactt tgctatttac caacattgct
    16261 gcttttctct atgcacgctt tcgactaaca aaagctactt ttcttgattg tttaaaaaca
    16321 ttgatctatt caatttcatt gcctactata attgcaacaa tcttgatggt ctttcttcct
    16381 tcttttgaca ctagcgcctt cattgctttt gcaggaatct ttatttttgc tcaagctgtc
    16441 aaaggatggg caaaaatatc gatcaattaa gaaataaaga caactcgtta tttctacttc
    16501 aatagaccct tttagaggtt gtgacatttt tttgtcacag cccctttttt atttgaaaaa
    16561 tatttctttc tcaaaaaaca agcaaggcgc ttcaaagttc ttcttgtatg cgctacactt
    16621 agagtaaaga aaggcgggaa attaactatg tcagaaagaa tcatttatgc agatcgtcca
    16681 tttcgtgatg aatttatcga agaaatccat actatagcgc ctggttatat ctttaaaaca
    16741 gaattacaac cagaggatct acaaaatgta gagatcagtt tgggctggaa ctcagactac
    16801 caagaaaaac tattagcctc ttctaccttg aaatgggtcc aatctatctc agctggtgtt
    16861 gatcagctac cccttgagac tttttcaaag aaacatattt tactttcaaa tggtagtggc
    16921 atccacagtg aatcgattac ggagcatgtc ttaggaatca tccttgggta ttctcgtggt
    16981 ctttttcaag cacaaaaggc acagagtgac atgaaatggt taggttcatc ggtccattac
    17041 caatctatcc aagggaaaaa aatactaatc attggaactg gccatattgg aaaaatgatt
    17101 gcaaaaaaat gcaaagcatt agacatgaac tgctatggaa tcaatacatc cggtcatcca
    17161 gcagaaggaa tggaaaagac tttttcttta aatcaactaa aggaagttct tcctgaagta
    17221 gatttcgtag tcaatatctt acctctaacg gaggaaacaa ctgggctctt tgatcagcat
    17281 atgtttgaac actttgatcc aaaatctgta ttcatcaatg ttggccgtgg tgcttctgtt
    17341 aagacacaag atctggtgca ggctttaaat aaccagcaac tttcctttgc agcattagac
    17401 gtttttgagg aagagcctct tccacaagat catcctcttt ggaaaatgga caatgtactg
    17461 attacttccc acattgcagg tttgacacct gattttcaaa agaaattaat ggctattttt
    17521 ttaaccaatc tgaaaagcta ctttgccacc catgagcttc aaaccaatca agtaaaattg
    17581 tctgcaggat attaatagct gaaaacaata ttttcaaaag aaaaaaggta agcgccccat
    17641 agaacagtac ctgtacgtca aataacccga cctatagaat agaatcggga atgtttggcg
    17701 tttttagtta cgagaaaaat gttgttgtac atccttgttc caaggcaaca gttgatctaa
    17761 agtttctgct gaaagtacgg gaagatttgg tatcttatca aatagaaact ctagatactt
    17821 tacaggattc aatccgttaa gttctgctgt tttatataca ctcagaatga ttcctgaagt
    17881 tcgtgctcct tcaagacttg tggaatgcaa ccaatttttc cgtcctatca ccagctcttt
    17941 gatatttcgc tccgcacgat tatttgtgag ttctaaacga ccatcttcaa aaataggttc
    18001 aaaaccactt cgtagtttac acgcatattg aacggctgca tccaacttgg attttgcaac
    18061 aggatctatc gttgccatcc actcataaaa agcaataaat aatggacgca gttcttgttg
    18121 ccgacagctt aatcgcttct caggagaaaa gtctttccat ttcttttcta ggacaaacca
    18181 tttatccaat tgtttgactg cttgagctgc gggaatatcc gtgtttttct ttccttttgg
    18241 aattgcttcg aaaaatttcc gccgaacatg cgcgaaacaa tagacaagtc gtacactttc
    18301 cacggcatta tacccgctat agccgtcaca gtgtaaatac cctgtaaact ccttgagaaa
    18361 agttttcgga acatctccag cacgactttc gtcgtggtga taataaacga tgggatgctg
    18421 actgtctttt cctgtacaaa actgccagta ataggtatct gttttggcac tttcaatcac
    18481 acgataactt gtttcatccc cgtgtagaat ctcttcttga ttaagataga ctcttaatcg
    18541 ttctcctaaa gggtctaacg cgttttgcac tgctttaata tgccaattgg tgatattatc
    18601 tctcgaaata tccaacccat gtgtttgttt ccaatagttc tcttgccgat aggcaggaac
    18661 tttctgacca aatttcatcc ggatcgtttc cgccactaaa ctattggaac ctaagctatt
    18721 agaaatcaaa ggtttaggaa cgatcgcttt tttaaaagaa gaaagtcctt ctttttcgca
    18781 gcttttacaa caataggtat gttgataatg ggtgagacaa tccaacatag cctgatggaa
    18841 acgaatttct tctcgtactt ttgtccgtcc gatatctttc atctgtgaac cgcaatgttc
    18901 acaaaattgt tcttccaaag gaaggagatg ttctttctct gttataggta agtgtttgat
    18961 tttgtctgct ttcgttccta caactcgttg atgtcctttt actggaataa tggtggcagc
    19021 tgcttctgga acacttacag aaatttcttc atcactaaaa agattcaatt gcccattcgt
    19081 ttcctcaaag agagtctctt tagagcgtcc aaataatttg ttggttaaat aattgacctt
    19141 ttcattcaat agttggattt cagttgtaag attctgaatg gtttgttgtt gtgcttgatt
    19201 ggttttatgg gcttcttgaa gcaactgaag caacgtatct gtagtttcca aaatttttca
    19261 ccaccttgaa tcgaactgat tttgttacag aataccacaa gaaacgcaca agaaataggg
    19321 gttcaacaca ttttttgtgt taacccccca gcttaaaaaa ctcctttttt tcctggcaaa
    19381 atagctcgag gttgatcgat acttaatcct tctaacaacc atcgaacctg ttggttcgtt
    19441 aaggttcgaa cttcgtcttt cgtccgaggc cattttaact tgccgttttc tatccgctta
    19501 taacagagaa gaaaaccatc accgtcccaa tagagaagtt tgaaacggtc ttgacggttc
    19561 ccgcaaaata aaaaaacggc atcttcgtaa agctcgagtt gatactcttg ttgaataaga
    19621 ctggcaagac catcaattcc ttttcgcaaa tctgtttttc cgcacacaat ataaatatgt
    19681 ttggtttgaa gtaaatcata aagcattgga caacagttct ttcaaaatag cttgaagaat
    19741 ataattgtta ataccgttat agatcaaaac ttcagaacca ttctctcttt tgatgcgcat
    19801 cgctaacttg gcagccgcgg gtttaccttt ttctggtgca ctcttttcgt ttcctaattg
    19861 gacaggaaca atctcattta atttatacat agaaaacctc cagacttctt gatagctcaa
    19921 gtatatcttg aggtgcattt gttagt
//
LOCUS       DACDRN010000044        19577 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDRN010000044 DACDRN010000000
VERSION     DACDRN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.044"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..332
                     /locus_tag="IWJ80_001857"
     CDS             <1..332
                     /locus_tag="IWJ80_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9461572.1"
                     /translation="IDNLREEILLYTEGKEENDWLFPSRQQGRHITRDRVYQIYADIA
                     EKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQAITKRYIGITEDEIGASL
                     RGFKLGV"
     gene            complement(505..978)
                     /locus_tag="IWJ80_001858"
     CDS             complement(505..978)
                     /locus_tag="IWJ80_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9461573.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            990..1526
                     /locus_tag="IWJ80_001859"
     CDS             990..1526
                     /locus_tag="IWJ80_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9461574.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1566..1937
                     /locus_tag="IWJ80_001860"
     CDS             1566..1937
                     /locus_tag="IWJ80_001860"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9461575.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2221..3492
                     /locus_tag="IWJ80_001861"
     CDS             2221..3492
                     /locus_tag="IWJ80_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP9461576.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            3737..4762
                     /locus_tag="IWJ80_001862"
     CDS             3737..4762
                     /locus_tag="IWJ80_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9461577.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5056..6165
                     /locus_tag="IWJ80_001863"
     CDS             5056..6165
                     /locus_tag="IWJ80_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9461578.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6248..7117
                     /locus_tag="IWJ80_001864"
                     /pseudo
     CDS             6248..7117
                     /locus_tag="IWJ80_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7160..7984
                     /locus_tag="IWJ80_001865"
     CDS             7160..7984
                     /locus_tag="IWJ80_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9461579.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8016..9326
                     /locus_tag="IWJ80_001866"
     CDS             8016..9326
                     /locus_tag="IWJ80_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP9461580.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9474..10733
                     /locus_tag="IWJ80_001867"
     CDS             9474..10733
                     /locus_tag="IWJ80_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9461581.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            10743..11615
                     /locus_tag="IWJ80_001868"
     CDS             10743..11615
                     /locus_tag="IWJ80_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9461582.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11627..12457
                     /locus_tag="IWJ80_001869"
     CDS             11627..12457
                     /locus_tag="IWJ80_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9461583.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12497..14680
                     /locus_tag="IWJ80_001870"
     CDS             12497..14680
                     /locus_tag="IWJ80_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9461584.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            14741..15961
                     /locus_tag="IWJ80_001871"
     CDS             14741..15961
                     /locus_tag="IWJ80_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP9461585.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            15958..17418
                     /gene="gtfA"
                     /locus_tag="IWJ80_001872"
     CDS             15958..17418
                     /gene="gtfA"
                     /locus_tag="IWJ80_001872"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP9461586.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17495..18601
                     /locus_tag="IWJ80_001873"
                     /pseudo
     CDS             17495..18601
                     /locus_tag="IWJ80_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            18923..19132
                     /locus_tag="IWJ80_001874"
     CDS             18923..19132
                     /locus_tag="IWJ80_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9461587.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19125..19274
                     /locus_tag="IWJ80_001875"
     CDS             19125..19274
                     /locus_tag="IWJ80_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP9461588.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19309..19577
                     /locus_tag="IWJ80_001876"
                     /pseudo
     CDS             19309..19577
                     /locus_tag="IWJ80_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT         6680 a         2957 c         3813 g         6127 t
ORIGIN      
        1 ttatagataa tttgagagaa gaaattttat tatatactga aggtaaagaa gaaaatgatt
       61 ggttatttcc gagtcggcaa caaggtcgac atataacaag ggatcgtgtc tatcagatat
      121 atgcagatat agcagaaaaa ttaggacgag atgatatagg aacacataca ttaagaaaaa
      181 cttttgggta tcactactac aagaaaacta gagacattgc taccttaatg tttattttta
      241 atcatagctc gcaagcaatt acaaaaaggt acattggtat aactgaagat gaaataggag
      301 catcacttcg tggatttaaa ctaggtgtat aatttaaaaa tttttataaa cagtaaagac
      361 aaagattaaa agctattttt atctagaacg attttatttc ataaagttcg cggaataaaa
      421 gctggggtta ctggtacttg gtataggttc gtgttatata aactaagtaa ttaaataaca
      481 tttttaggtt ggaaaaggat gaaattaaaa aaagagaatc aatacggtct caaaaacgca
      541 aacgctgcgg aataagaaaa atactgataa ctcaacgttt agcagactat ctaataggga
      601 tagtctgctt ttttcgtttt tgaccatact tttgaccttc tgcaaacttc cttgcttttc
      661 ctctgtagaa ctcatgaaat ctgcaaaacg aattgccgtt tcctctgtct gctttttcgc
      721 gatatgggtg tatatatcat ccatagtggt tttaatatct gtatgtccaa gtcgaacata
      781 cacttctttg acagacgcac cagattcaaa gagcagacta caatgggtat gacgaaaacc
      841 atgaagtgtg atatgtttta agttattctc tttaatgatg attttcaaat tgtgatttac
      901 atagtctaaa taaaggggct tattttcttc tgatgaaaac aaaagttgtt caccatcttt
      961 tataggtttg attctcattt ttagatatta tgcccgcacg tattcatcac atttctattg
     1021 tgaatcgctt tattcgaccg acatttgact tttatcataa tatactcggt ttaaaattac
     1081 taatgaaaac aattaatcaa gacgatcata ccatgtatca cctgtttttt tcagataatc
     1141 accaccgtgt gggaactgaa ctgacttttt ttgaagtaca agaagggaac aaccagtttt
     1201 ttggaaccaa tacgattgaa cgaacgatac taaaagttcc ttccgaagca tcattgcatt
     1261 tttgggaaat atattttgaa acacaaggcg tttgccatta tggaatcgaa tcattttctg
     1321 gacgacctat cttacgtttt gaagggccag atgcaacacg attagcttta gttcccttac
     1381 gcgaatttga agatattgat gattatttcc cgtatgtcac agaccagatc cctacagagc
     1441 atgccatttt aggaattgat gccattcaac tacgcgtaca atattccgat gcgactgaac
     1501 gagagttatt gtcggtaggc tggtagcaca aagaatacgt cagtttcttt tcgggtcatc
     1561 aacgagtgac aatcttagaa aatcaagaca ctcagttcta tcaagaagtt cagattattc
     1621 aagatcgtga gaatccagtt gccattgaag ggattggtag cgtccatcat gttgcgtttg
     1681 gcgtagaaaa taaatctgat ttacaacgca tcgacaaaca attacaagag cgcaatttta
     1741 taaattcagg aataaaagat cgtgaatttt ttgtatcact ttattatcgt gacccaaatc
     1801 aattattgat tgaaattgct acaggggaag gaaacctaga tgcaaaagcg tatgaaaatc
     1861 aaagtccacg ctttgaagaa attcctttat ttttaccgca gtacttaaat ttcattcgtg
     1921 aacaagtgga gcaataatta gctaatcaaa tccatgacga agagtaagag gttgactcat
     1981 aagtcaaaaa ataaagaaaa acgcatcaaa tccattctaa agaatgttga tttgatgcgg
     2041 ttcctatttt tataaactat cattttcgac taataaaact acttatgagt cagcctctct
     2101 ttttttcttg atttttatac ctctttctag gtaaactaaa catctgccaa aaataccgtg
     2161 tctcattgaa acattctcaa ctatttcaaa ataaagggga gtaccaaatg aacgagtata
     2221 ttgaaattat ccacgcaaga gaaacaactt aaaaatgtca gtctcaaaat tcccaaaaat
     2281 aaaattagta tttttaccag cgtatctggt tcaggaaaat catcgattgt ttttgatact
     2341 attacccaag aagctggtcg tcaactgaat gacacctatg atagttttac ccgtttattt
     2401 ttacctaaat acagtcgtcc agaggttgat gagattgata atctttctac ggcgattgtg
     2461 gtcaatcaaa agaagattgg tggcagtgcg cgctcaacgt tagggacgat tactaacatc
     2521 aatgcgttat ttcgtgtgtt gttttcatgt tttgcaacac ctagtttagg atatgcaaat
     2581 gcttattcat ttaatgatcc tagtggaatg tgttctgtct gtcagggaat cggtaagacg
     2641 attgcatttt taataggatt gctttatttt tcggctattg ataatgctga ttttgtattt
     2701 tctgatccat ttaccgcaga agttttgtct ggaattgagg aacaggcaaa gaatcatggt
     2761 tattttgtat tggtgcataa tgttaattca gcagaagatg ttaaaactat tcagagaaat
     2821 tggcgttttg aggggttcat tgttgttggt gtacgtgtaa aagaattttt ggaattaaat
     2881 aacgtttttg atggtcctgt tgtttatatt gatacgcatc tttcaaaaga gacattaaga
     2941 gaacttgaac aacaaatgaa tcgtttattt gtaaatacaa atgattacca agctagtaca
     3001 ttggcagtgg aacatttgat tcattatggt catgagaaaa tagcgtttct ttcttttgac
     3061 tacattgtag gtgaaccaag tgttattcaa gaacgacatc aagcttatat cgatacatta
     3121 aagaaatatc atttaacctt taaatcgcat tggatatata acaactctga atttgagcga
     3181 atttatcaag aattagctga atttactgca attgttgtta ctggagatta tttagcggct
     3241 aaattagtaa aatttttaaa aaataagaat aaacgagtga tggaacaatt atcgattatt
     3301 ggatttgatg atattacttt tgcagaatta atggatcctg cgttgacaac aatacgacta
     3361 aatccaggta acaagggggc gattgctttt caccaactga ttggactggc caaacaagaa
     3421 gaaataaaaa atcaagtact actattagag ggagaattga tttctcgaga ctctgttcac
     3481 aaaatcaaat aaaaaagctc tgtgaaataa gtatagttaa caataaaata tccgaacgat
     3541 agagagaatg aactgtgatc tagcagttcc tcgtcgcaaa ttcactagaa ttagcattat
     3601 ttactctaaa ttgttcggat attttttctt gtttttaatt atgattacag tgcctatttt
     3661 taacgaaatt tgcttatttt caaaatgtat gattatatgt tagtaaacac tttcaaatat
     3721 gaaaagagga gctattatgg taggaattag ggatgttgca aaacgggcgg gtgtgtccat
     3781 tgcaacagta tcgcgcgtgc taaatgagga tccgacactt tcagtaaccg atgaaaccaa
     3841 acgaaagatt gcagaatcgg ttaattttta tcattatgtc aaaaaaagtc ccgttgcaaa
     3901 aaatttaaca aatattgcat taataacgac agtttctgaa gtagatgagt tggaagatcc
     3961 ctattttcga acgattcgca gagggattca agtcgaagca gaacaaagca aagtcaattt
     4021 gaagaaaatt attcgtttgt cagaaacacc gttggagcaa gacgatttgc taaaatgtca
     4081 aggtgtgttg attattgggc aggtgctatc ttctgtgatt gaagaagtat cagcaattaa
     4141 ttcgaatgtc gtagtgattg atgaccctag tattgaagct gaagtagatg cagtttatac
     4201 tgatttaaca aaagcaacga atcagcattt agatcgattg tataaaaaag gacaccgaaa
     4261 cattgcgttt atcggaggag gacgtgttcg tttagatagc caaggaaata aacacgtgtc
     4321 agatgatgat tatcgaaaaa tagcctatga gacgtggatg aaacaaaaag gattagaaaa
     4381 atatagtcaa atttatttag aaggctggac cacacttgac ggaatgcagg cagcagattt
     4441 acttttgaaa aaacatgcta aagataagtt gccgactgct atcatggtag gaaatgatcc
     4501 tattgctgtg ggggtttatc gttcacttca aaaaaatggt ctacatattc cagaagatat
     4561 ttcgattgtg agttttgata atattgaagt tgctgaattt ttaacacctg cattaaatac
     4621 ggtgaatgtg aatacagaag aaattggaaa actagcagtt cgcatggtta atgaacgaat
     4681 aaaagaagtt agaaatgttt cgatcaaagt gattgtttct aataatatta ttgttagaga
     4741 aagtgaaaaa aatatcgatt aaaaaagtaa acatccaaaa aagggtgttt acttttttgt
     4801 ttactaatat ttttagtaaa aaatctcgac aaaacattaa gtaaacagtt atggtgttgt
     4861 taacaaaaag aaaacgcatt caaaacagga ggaattaaga tgaaacattt caaaaaaata
     4921 gctttaacag ggatagctgt tttaactggg acattactcg cagcatgtgg tgggggagat
     4981 agtaattcta aagacgggca agtaacttta agctatgcga gttgagatac tgaccaagca
     5041 ccaggactaa gaaaaatgtt agatgaattt gaaaaacaaa atcctgatat caaagtagaa
     5101 atggagacaa cgccttggga tcaatattgg gttaaattag aagctgcaac aacgggaggt
     5161 aatatgccag atgtagtaac catgcactct agcgaatcct ataaatatat gtcacagaat
     5221 gctttgatgg gacttgatga tgttatcaaa gaaaatgata ttgacatggg aaactttact
     5281 gaaggaattg ctgatttcta tacgcttgaa gacgaactat acgcaatccc taaagatgct
     5341 tctgtagtgg ggctatggta caacaaagaa ttatttgata atgcaggtgt cgattatcca
     5401 gatgaaacat ggacatggga aacactcaga caagctgcaa ttgatttaac agatgaatca
     5461 aaaggcatct atggttttgc atcacatatt ggaacagaga ctggctactg gccatttatt
     5521 tatcaaaatg ggggagaagt atttgccgat gacaatacaa aatcaggttt gaatacccca
     5581 gaagcacaag aagctttagc tttttatcga gatattgcac ttgttgatca tgcttcacca
     5641 acaattacag aattacaaga tcctgataag ttaagtcgtt ttcaatcagg tagattagca
     5701 atgatgattg aaggaaactg gtttgcaccg ggatttttga aaaatgacta tctagttgaa
     5761 aatgttgctt ctgccgtgtt acctaaggga aaagttaatg caacaattac aaatggactt
     5821 ggttgggctg ccagtgcgag tacaaaatac ccagaagaag tgaaaaaact aatggcattt
     5881 ttagctagtg aggacgccaa taaaatccaa gcggaaacgg gggcatcaat cccagcacta
     5941 aaaggctttg aagacgagtg ggctgcttct tcagatacaa ttgatttgag tgcttttgga
     6001 aaaatgttgg agtatggagt ccctcgtcca tacaataaag atggattacg agctgaacaa
     6061 gcagaagttc aaattattaa ccgtattctg tctggtgaag taactgttga agatggaacc
     6121 gctcaaattg aaaaagaagt caatgcgata ttgagcggaa attaaattag ttaattatac
     6181 aatatacagg cgaaaggatt ctatttcgtc tgtatattaa tggatgaata ccaaaaaggg
     6241 ggatattatg aaaaatcgaa tttttggaaa atatcgtaaa gatcaagtga tatggggcta
     6301 tatttttatt tttccaacgg cattaggatt aattttatta aatatatggc cagcaattca
     6361 aacagcaatt ttaagttttc aaaaaacggt agggttcggt gatactgaat gggtcggatt
     6421 aagaaattac caaaaaatgt ttgcagacgg tgaagttttt cagtctttat taaacacatt
     6481 aatttatgca gtggtttcga tacctagtat tgttgtatta tccttgttgg tggcagtttt
     6541 aatgaataga aaagttaaag gattgtcgat ttatcggaca atttattttc taccagtcgt
     6601 tgctgcacca tccgcagtcg caatgatttg gcgctggatg ttcaataatg attatggact
     6661 gattaataac atgttggcta aaataggtct cgatggacca gcatggttaa cggatccatc
     6721 gattgcgatt tattctatta tcattgtagg agtttggagt gctattggtt ataacatggt
     6781 gttattacta gccggcttat aagaaattcc taaagattat tatgaagcag cggaaattga
     6841 tggtgctagt ccaatccagc agtttttcaa cattacgtta ccattagtta caccaacatt
     6901 attttttgta gttgtaacaa cagttatcaa tgctttccaa gtatttgatg ttatttttat
     6961 gatgattact cctaatagta ccgccatgtt taaatcacaa tcattagcct atttattcta
     7021 taaacattca tttattttaa atgacaaggg atatggttcg gcaatcgtta tgttcctttt
     7081 agtgattata ttaattatta ctgcaattca aatgtagttg cagaaaaagt gggtaaacta
     7141 ttaaggaggc tatcaaatta tgaaaaataa atttttttca aatgcgccaa tccatatcat
     7201 gcttatttta ggtggtattg caatggcatt accttttatt tggatgattt taacttcagg
     7261 gaaaacgttg gttgaatcta cccaaattcc acctacaatt ttccctgaga aatttcagct
     7321 aagtaactat caacaagtac ttgatttatt accattcatg gctttttata tcaatacatt
     7381 tttaatggtt gttggacgag tgattggctc ggttttattt tcagcaatgg ctgcatatgc
     7441 ttgtgcgcgt ctaagttttc cagggaaaaa tatccttttc agcttagtac ttattcaaat
     7501 gatgattccc ggttccattt ttattactcc acagtattta ttagcacaaa aattgggtat
     7561 tttaaataca attccagcat taatattacc aggggtagtt agtgcgtttg gtacgttttt
     7621 attaagacaa tttttcttag gtttacctga tgaattggag gaagcggcct atttagaagg
     7681 tgccaatatt tggacgatct ttttcaaaat tatgttgccg cttgctaaat caggattggt
     7741 tgcacttgga atattcacag cgttgtttgc atttaaagat ttaatgtggc cattgattgt
     7801 caatacttca attgataaga tgcctttagc tccgggatta gcaaacttac aaggacaata
     7861 tgccacgaac ttcccacaat taatggcagg ttcgttatta gcaatttggc caatagttgt
     7921 gatattcatc gttttccaaa gaaaattcat tgaaggaatt gctacaacag gtagtaaatt
     7981 ataaaactat taattagtga ttggagagta agtacatgac aaaaattaca tttattggtg
     8041 caggaagtac tatttttgca aaaaacgtat taggcgatgc gatgttaact ccaagtctac
     8101 aagatgcaca tattgcattg tacgatattg atgaaaaacg actaaaagaa tcaaatttaa
     8161 tgcttaaaaa tattaataaa aattcgaatg aaaatcgtgc tgatattgtt tcttattatg
     8221 atcgcaaaga agcgttaaga gaggctgatt ttgttatcaa tgctattcaa gtgggtggtt
     8281 ataaaccttc aacagtgatt gattttgata ttcctaaaaa atatgggttg cgacaaacta
     8341 ttggtgacac catggggatt ggtgggattt ttagaaccct acggacacta ccagtaatgt
     8401 tagactttgc taaagaaatg gaagaagttt gtccaaatgc ttggttattg aactatacaa
     8461 atccgatggc gatgttaacc atgggtgttt taaaagcgac gagtattaag actgttggtt
     8521 tatgtcatag cgttcaagta tgcgtaccag aattgtttga gcatttagga atagccgatc
     8581 aatatgactt aaatgacttt caatggaaaa ttgcaggcat taatcatatg gcattcttgt
     8641 tggaaattac aaaaaatggg gaagatttct atccaacaat tcgtcagtta gctgcagaaa
     8701 aaccaaatcc acatccagat tctgtacgtt ttgaattgtt gaaacgtttt ggttactata
     8761 ttacagaatc aagtgaacac aatgcagaat accatccata ctttattaaa tcaaaatacc
     8821 cagaattgat tgacgaatta gggattccgt tggatgaata cttacgtcgt tgtgaacaac
     8881 aaattaatga ttgggaaagc atgcgagagg acattgtaaa taatggggag ttaacacata
     8941 cgagaagccg tgaatatgct tcatatatca tggattctat tacgacagga actccgtcag
     9001 tgattgcagg aaatgtctta aataaaggct taatcacaaa cttaccgaca gatgtttgtg
     9061 tggaagtacc ttgtttagtc gataaaaatg gcgtgcaacc aacctatgtc ggtgaattgc
     9121 caacacaatt agctgcttta aatcggacga atatcaatgt tcaagaactg acagtagaag
     9181 ccgctttaac actagataaa tcaaaaattt atcaagcagc gtatttagat ccacatctat
     9241 catctgaatt gtctttaaca gatatcaaaa atttagtgga tgatttaatc gaagcacatg
     9301 gggactattt acctgaatat aattaaaaag aagctgaaag agtaaggaat tttcaattcc
     9361 ttactctttt cttagttaaa tttagtaaaa atattgacaa aacacttagt aaacagttac
     9421 attagtgtta acaaaaagaa aacgcattca aattgtataa ggaggagcat aaaatgaagt
     9481 ggtctaaaaa aattatttta gcttgtatag cactaggagc aacgatctct ttgactgctt
     9541 gtggaaaaaa tcaggaagat gcaagcggta aggtagaaat agagtttttt aatcaaaaaa
     9601 aagaaatgac tcaaacgatt caggagattg ctaaagattt tgaagcaaaa aaccctgata
     9661 ttcatgtaaa agttgtggat gtaccgaatg ctggtgaagt aattaaaaca cgtatgcttg
     9721 caggcgatgt accagatgtc attaatcttt atcctcaaag tatagaattg aaagaatggg
     9781 cgaaagctgg ttatttagag gacttaaccg aagaaccgta tttagagaat atcaaaaatg
     9841 gatatgcaca acgttttgct attgaggatc gtgtatatag cataccatta accgctaatg
     9901 tctatggttt ttactataac aaaactgctt ttgaggaaat ggggattcaa gcaccagaaa
     9961 cttgggcaga atttgaaaaa atagtagctg agattaaaga tcaaaacaaa gtaccgtttg
    10021 cgattgcagg agcagaaggc tggactttaa atggttatca ccaactagct atagcgactg
    10081 ctgctggtgg tgaagaggaa gcgaacaatg tttggcgttt ctcagaagtt aatggaatta
    10141 atgctgaaag caaagaaatg caaaaagatt ttaatcgatt agatttgtta agagaaccaa
    10201 aagctttgca aaacaactgg caaggtgccg gttataacga tacagtagtt acttttacaa
    10261 aaggtgatgc gttgattatg ccgaatggtt catgggcaat gccgatgatt cattcacaaa
    10321 atccagattt tgaagtagga acttttcctt ttccagcaga tgaagcaggt caaagtctaa
    10381 cgattggcgc aggagaccta gccttatcag tttctgcaac ttctcaacat aaagaagaag
    10441 ccaaacgttt tgtagaatat atgacaacac ctgaagcgat gcagaaatat tatgatgtag
    10501 atggtgcacc gtgtgctgtt gagggcgtta tcgagaatac tcaggattca ccattaagtg
    10561 gtttaactga acttgctttt actgatcgtc atttagtttg gttagccaaa gattggaata
    10621 gtgaaaatga tttttatacc ttaacaacaa attatttaca caatggcaat caaaaagcca
    10681 tgattaacgc attaaatgct ttctttaatc caatgaaagc tgacggaagg taggaaaata
    10741 aaatgaaaat aaaagaaatt ttaaatcgtt actgggcaat catttttgta gcaattccaa
    10801 ttattttgca gatgattttt ttctactttc caatggttca aggtgctttt tacagtttaa
    10861 ctaattggac tggtctgact tataattttg attttgtggg tttgaacaat tataagattc
    10921 ttttttctga tccgaagttt atcaaatcga ttggcttcac tttaattgtg acgctttgca
    10981 tgattgctgg acaaattggt tttggtattt tgattgcgcg ggcgttgaac gcaaatatta
    11041 aagggaaaac attctttaga gcatggttct tttttccagc ggtcttatcg ggcgtggttg
    11101 tttctttagt atttaaacaa atttttaact atggtttacc aaaaattggt agtatgttag
    11161 gcattgaatt tttacaaacc agtcttttag gaacaacatg gggagcgatt tttgcagtaa
    11221 tttttgtttt actttggcaa ggaattgcca tgccagtcat cattttctta gcgggttttc
    11281 aaagtattcc acaagaaatt attgaagctg ctagaatgga tggggcgaat gataagcaaa
    11341 ttttttggaa tattgaaatc acctacttat taccaagtat ttcaatggta tttattatgg
    11401 cattaaaagg tggcttaaca gcatttgatc aaatttttgc tttaactggt ggtggaccaa
    11461 gcgatgccac aacatctttg gggttacttg tttataatta tgcttttaaa aacaatcagt
    11521 ttggttatgc gaatgctatt gctttagtct tatttatctt aattgggatt gtttcactgg
    11581 cacaattaaa attatcgaaa aaatttgaaa tctaggaggt ttcccaatga aaacatcaaa
    11641 agaaaaaaat atttctaaat acttgctttt attattaggg ggcttcatga ttttagttcc
    11701 cttagtttca acgctattcg gttcatttaa aacaacaccc gatattatga ataatttctt
    11761 tcgttttcct aatccagcga ctttggaaaa tttccgaaga ttgttagcgg atggaattgg
    11821 tcattacttt tggaattcgg caatcatcac aatcattgca gtagttgctg tgacagcaat
    11881 tattcctatg gctgcttatt cactagcaag aaaaatgact cgtgggaaag catacggatt
    11941 gatgtattta tttttaattt taggaatttt tgttcctttc caagtaatta tgattccgat
    12001 taccgttatg atgagtaaaa ttggtttagc aaatatgtgg ggattaatct tactctatct
    12061 cacttatgcc gtaccacaga cactattttt atatgttggt tttattaaac taaatgtgcc
    12121 tatcagtctg gatgaggctg ctgaaattga tggggctagt cactttacaa cttatttcaa
    12181 aattgttttc ccaatgttaa aaccaatgca tgcgacgact attattctta atgctctatg
    12241 gttttggaat gatttcatgc taccattatt aattttaaat cgtgattcta atatgtggac
    12301 actaccattg tttcaatata attatacggg acaatatttt aacgactatg gtccaagttt
    12361 tgcttcctat acagtaggaa ttgtcacgat tacactagtt tatcttgctt tccaaagaag
    12421 tattatagct ggaatgagca atggagcagt gaaataacca ataaattatt ctcattataa
    12481 aggagttcat aaacttatga ttatatttga caatcacaca caaacatttc atttacaaaa
    12541 taaaaaaatt agctatctat taggaatcga agaaaataat tatttaacgc atctttattt
    12601 tggaaaaaag attacgcact attctggtgg gtatcgctac ccccgaacag atcgttcctt
    12661 ctcacccaac aatgcaaatg cgcaagatcg tttattatct ttagacacat taccattaga
    12721 atttccagga tatggacatg gtgattttag agaaccggcg cataatatta aattaggaaa
    12781 cggtagtcgt gtaaatgatt ttcgttatga tagctacgaa attcaaaaag gaaagcccag
    12841 cttagcggga ttgccagcct tatatacgga agatgatgat gaagctgaaa cgctgattat
    12901 tcaattgaaa gacgcgatta gtggattgac tttacagttg ttctacacaa tttatcaaaa
    12961 tcacagtgta attattcgtt ctacgcaatt agtaaataat agtgatgaaa cagtggagat
    13021 taaccgctta gctagtcaat caattgattt tccaaatcgt ccactcgagt taattcattt
    13081 aaatggtacc tgggcaagag aacgtcaaat gactagagaa aaaattcata cagggataaa
    13141 agtattagat agtaaacgcg gatcaagcag ccatcaacaa aatccgtttg ttgctttagt
    13201 agatgccaaa acagatgaat ttcaaggaga ggcatatgga tttaatttag tttatagcgg
    13261 taatcacgaa acggtgattc aaaaagatcc atttgaccaa acacgggtca ttatgggaat
    13321 taattcattt aattttggtt ggcaactaaa aactggcgac tcatttcaaa cgccagaagt
    13381 cgtgatggtt tatagtaatg aaggtctgaa tacgttatca cagacgtatc atgaattgta
    13441 taatcaccgt ttaattcgtg gaaactatcg tttaaaagaa cgtcccacgt taatcaacaa
    13501 ctgggaagca acgtattttg attttaacga agaaaaaatc atgaatattg tcgatgatgc
    13561 gaagaacttg ggcattgaaa tgtttgtttt agatgatggt tggtttggcg gtcgtgaaaa
    13621 tgacttcact tcattaggtg attgggttga aacagaaggc aaacttgctt gtggtttgga
    13681 aaatttagcc aagcaagtgc atgataacgg gatgaaattt gggatttggt ttgaaccgga
    13741 aatgatttct gaagatagtg aactatttcg agcacatcca gattgggcat tgggtattcc
    13801 aggtcgtgac cgctcaatta gtcgaaatca atatgttctt gatttttcaa gacaagatgt
    13861 tcgtgataat atctatcaac aaatgacggc gatttttgat cgtgtaccga ttgattatgt
    13921 gaagtgggat atgaatcgaa atatgacaga agtctattct gtcttgttgg actcaacgat
    13981 gcaaggtgaa gtggctcatc gttatatgtt agggttatat gaatttttag aaaagttaac
    14041 aacgaattat ccaactattt tatttgaaag ttgttcgggt ggaggcggtc gttttgatgc
    14101 aggtttcttg tattacatgc cacaaacttg gacgagcgac aataccgatg ccgtggctcg
    14161 tctgaaaatt caatatggaa ccagcttagt gtatccaatt tcaagtatgg gtgcgcatgt
    14221 ttcagcagtc ccaaatcatc aaacaggaag agaaacgagt ttaactattc gtggcaatgc
    14281 tgcgatggct ggtgtttttg gttatgagct agatctaacg caattaactt ctgaagaaaa
    14341 agcagaaatt aaagaacaag ttgcctttta taaaaagcat cgccaacttt tgcaatatgg
    14401 aactttttat cgcttggaaa gtccgtttga aggcaatgat actgcatgga tgtttgtctc
    14461 aaaagaccaa acagaagcca tggtctttta tttccgagta ttagcagaag ctgcgcatcc
    14521 attagtcaca ttgaaattag caggattagc agaagaagca acttatcaaa tggaagatgt
    14581 aacatttgct ggcgatgaat tgatgaatct cggtttttat attgactcag aactacatgg
    14641 tgattatgcg acacaacgct tttatttgaa acagatatag tagcttgaat ttacaatata
    14701 agtgcaacac caaaagagac tcattccaaa cgaagaaaat atgttttatt tacacgcatt
    14761 tactaaaaag cagcctgatt tgaattggga aaatgaagaa gtacgtgaag aaatctatgc
    14821 aatgattaac tattggttag ataaaggact aggcggattt agaatcgatg cgatattaaa
    14881 catcaaaaaa aaactagagt atggcatctt tgaatctgat ggagaagatg ggttagcctt
    14941 cattgggccc tggattctta atcagtcggg cattgaagtg tggttaaaag aaatgagaga
    15001 gcggacattt aaaccacata atagtatgac ggtagcagaa gccgatgttc ctaatgaacg
    15061 tttagatgaa tatattggtg aagatggcta ttattcgatg gtttttgatt ttagttatac
    15121 ggatattgat gtacctgaaa ctggtgaatg gtttaaagat agccaatgga catggacgga
    15181 tatgcgtacc aatattttta cgaatcaatt agttacgcaa gataaaggtt ggggcgcctt
    15241 atatttagaa aaccacgatc aaccacgttc aattaataaa tatattccag aagaatggat
    15301 caatgattat agtaaaaaaa tgttggcgac gctttttatg ttactaagag gtaccccttt
    15361 tatttatcaa ggacaagaac taggaatgac caacatagaa atggcatcat tagaagattt
    15421 tgatgatgta gctacgcatt ctcagtataa acgggcatta gaatatggat tggcaccaga
    15481 gcaagcattg aaagctgtca ataaacgaag tcgggataat tctcgcacac ctatgcaatg
    15541 gaatacgcaa aaaaatgcca gattctcaac ttctgataat gtatggttaa aagtaaatcc
    15601 taattatcca gaactcaatg cagaagctca gttaaccaat caacagtcgg tattcaatta
    15661 ttatcatcaa ttaattaaat taagaaaaaa tagtgtatac aaagacgtgc taatctacgg
    15721 acaatttcta ccagtgaaag ataaaaatga acatattttg ctttatgaac gccaattaga
    15781 agataaaatc gtattagtta tcttaaacgt tacagcaaca gaacaagaat attatgtaga
    15841 tgaacagtac caacaagtat taatcaataa ttatgaagat gttttattag atcaaagtcg
    15901 aaaattgcgt ttacgaccgt ttgaaagtat tgtattagct aattatggag gacaattatg
    15961 aaaattaaaa acgaagcaat gctcatcacc tatgcagata gtttaggaaa aaacatgggc
    16021 gaattaaaac aagtcttaga caaacattta caaggtgtta ttggtggtgt acatctgttg
    16081 cctttttttc catcgacagg tgatcgaggc tttgcgccga gtgattatac gacagttgat
    16141 ccagctcttg gaacatggga agatgtggaa gcattaggtg aagattatta tttgatgttt
    16201 gattttatga ttaatcacat ttctcgtgaa tcaaaatttt tccaagattt caaaaaaaac
    16261 catgagcagt caccatacaa agaaatgttt atccgtattc atgaattttt cccagaaaat
    16321 cgaccaacac aagaagatat tgatttaatc tacaagcgta aagacaaagc gccgtttcaa
    16381 acggtagaat ttgcggatgg tacgacagaa gaggtttgga atacctttgg ggaagaacaa
    16441 attgatttag atgtgaccaa agaagttgtg aaagaattta ttcgagaaac catcaaagat
    16501 atggcgaacc atggctgttc tttaattcga ttagatgcgt ttgcttatgc gattaaaaaa
    16561 ttagatacca atgatttctt cgtggaacca gatatttggg aacttttaga cgaagtgcgt
    16621 caagaagcag cgaaatatga cgtcgaatta ttaccagaaa tccatgagca ttattccatt
    16681 caaatgaaaa ttgccaatca tgattattat atctatgact tcgccttgcc aatggtcact
    16741 ctgtattcac tttatagtgg aaaatcagaa cgtttagcca attggttgaa aatgagtccg
    16801 atgaagcagt ttacaacatt ggatacgcat gatggaattg gtgttgtgga cgctcgtgat
    16861 ttattaacag atgaagaatt ggattacacc tctgaggaat tgtataaagt cggggcgaat
    16921 gtgaagaagg tctattcaag tgccaattac aataatttag atatttatca aattaacagc
    16981 acctattata gtgcattagg cgataatgac cgtagctatc tgttagcacg tgcgattcaa
    17041 gtatttgcgc cagggattcc acaaatttat tacgttgggt tattagcagg aaaaaatgat
    17101 attgaactgt tggaagcaac gaaagaaggg cgtaatatta atcgtcatta ctatgattta
    17161 gccgaaattg aagaagaagt gcaacggcca gtgattcaac aattatttgc acttttaaaa
    17221 tttagaaatg aatctgcggc gtttgatttg gatggaacga ttgaagtagc aacgccatca
    17281 gaatcagaaa tagtgattac acgtaaaaat aaaaacggta gtgatagtgc aacattaacg
    17341 gctaacttgg atacgaaaga atttatcatt gagtctaaag atataaaaat tttcactagt
    17401 gttgcattaa aattttaacc tattccatag aacaaaaaat cctttatact agcacttgga
    17461 tgacttatcc aagaccaata aaaaggactc atacatggat aagtataaaa caaatacagc
    17521 atttaataaa tggttttctg ccataaaatt aggaaaactt tctgtcaact cccaaaaatg
    17581 tatttttgat tacaattcgt atcgtaaaaa actgtctttt gaagctgtgc tcaagctctt
    17641 tctctacgca gttaatgaag aaaaagaaag tttgcgtgat ttgagtacag ctcttatcga
    17701 ccgatcgctc caactagaaa ccgatgtgtc atcgattagt catacccaat tgtctcgagc
    17761 atttaatgca cttgaaccta aagtgttaga agaaattttt caacaactgt tagaaaaagt
    17821 tcaacatcaa atcaaaccaa ccaagcgaaa tcatctgtat ctgattgatt ccagtacgtt
    17881 ttcgcttagt ttaaaacgtc ataagtgggc gactttccgt caaacaaaat caggtgtcaa
    17941 gttacactta aactattgtt atatggacga tactacgatg tatccaactg attttacctt
    18001 aacaaatgcc catgaacatg atctcaatca actaccagta ttggtgaatc aaccagaggc
    18061 aacttacgtg tttgatcgtg ggtattagga ctttgaaaag atggatcaga tgcattggga
    18121 tggctatttc ttcgtcactc ggattaaaaa aaacaccaaa gttcacgtga tagatactct
    18181 ggaaacatcg ccagaaacag aaattctacg agacgagctg gttcgtttag gatctaaaac
    18241 ctatctaacc gccaatttcc gactagtcac tgttcaagat aagaacggac gtgtgtttca
    18301 atttatcacc aatcgaatgg acgtctcttc aaaagaaatc tctgatatgt atcacgctcg
    18361 ttggcaaatc gaattgtttt tcaagcacat caaacaacac atgaccatta aaaccttctt
    18421 ttctcaaagt gaaaaaggcg tgcagaatca attgatttta acgatgattt ccgctctcct
    18481 cacgtttctc atcaaattgg agacgaaaac ggaaaaatca gtgttccaaa tcaaacgatt
    18541 ctttcgttat ttactgtttc agccctttga atgttgtcta gaaaaattaa ttcccacatg
    18601 agaaaaaaga aatttggctc gttacttttg gttttcagtt gaataacaga taaaaaattt
    18661 tctggaaaaa gtcatcctct gcgtcagcac agtttttttt gacttatttt cagaggaaat
    18721 agttttcaga atattctaaa atgttttatg caacactagt gatatttttt agaattagat
    18781 ttctaatgtg cattttttga gtattaagga gcaagtatac aaagatttat aaagctgatt
    18841 aaatttgaat aaaaatatgc aggaacgcta ctatttttag tagtgttctt ttttatattt
    18901 gaataaaaaa tgtggtggtt aaatggtggt tataacaaaa aatgaattaa tgaaattagg
    18961 ttatgggaag tatcagtctg agagaatcat acgacaggca aaagcaatta tggtttcaaa
    19021 aagctatacc tattacgaaa atagaagatt aggactagta cctgttgaat ctgtagaaga
    19081 agttttaggt atgaagatag aggtagagga ggattttcag aatcatggtt aattttaaaa
    19141 aagataaaaa tgggaaatat cattttactg ctagtttggg attcgacaaa cgaacaggga
    19201 aaagagtaca aaaaaacgta gaggggttaa aacagttaaa gaagaaaaaa agcgtatacc
    19261 gaacttgttt ctaattatgg aaaagaagca gaaacttctc aaagtactat gttgtttgag
    19321 gagttttttt ataaattatt cctaccttat tatcaaggga aagtagaaga aagaacatat
    19381 aaaaatcggt tatctacaat caaaaaaact tttcttattt ctttaaaatg gagttaagag
    19441 aaatacgtcc aattgacatt caaaagtggc aaaataattt attgagcaaa tataaaaatt
    19501 cttatgtaag aaacatgtat gggatttttc agatgtcatt agatcgagca gttgttttgg
    19561 gactacttga aaaaaat
//
LOCUS       DACDRN010000045        19477 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDRN010000045 DACDRN010000000
VERSION     DACDRN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.045"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(63..1442)
                     /gene="brnQ"
                     /locus_tag="IWJ80_001877"
     CDS             complement(63..1442)
                     /gene="brnQ"
                     /locus_tag="IWJ80_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9461589.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            2148..3119
                     /locus_tag="IWJ80_001878"
     CDS             2148..3119
                     /locus_tag="IWJ80_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP9461590.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            3143..5335
                     /locus_tag="IWJ80_001879"
     CDS             3143..5335
                     /locus_tag="IWJ80_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP9461591.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEVQEE"
     gene            5352..5828
                     /gene="ybeY"
                     /locus_tag="IWJ80_001880"
     CDS             5352..5828
                     /gene="ybeY"
                     /locus_tag="IWJ80_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP9461592.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            5806..6207
                     /locus_tag="IWJ80_001881"
     CDS             5806..6207
                     /locus_tag="IWJ80_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP9461593.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            6222..7121
                     /locus_tag="IWJ80_001882"
     CDS             6222..7121
                     /locus_tag="IWJ80_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP9461594.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            7264..8076
                     /gene="recO"
                     /locus_tag="IWJ80_001883"
     CDS             7264..8076
                     /gene="recO"
                     /locus_tag="IWJ80_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP9461595.1"
                     /translation="MSQLVESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            8460..9377
                     /gene="glyQ"
                     /locus_tag="IWJ80_001884"
     CDS             8460..9377
                     /gene="glyQ"
                     /locus_tag="IWJ80_001884"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP9461596.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            9379..11454
                     /locus_tag="IWJ80_001885"
     CDS             9379..11454
                     /locus_tag="IWJ80_001885"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP9461597.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRVSRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQITAMALSL
                     ASLDQIITK"
     gene            11838..12617
                     /locus_tag="IWJ80_001886"
     CDS             11838..12617
                     /locus_tag="IWJ80_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461598.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            12658..13602
                     /locus_tag="IWJ80_001887"
     CDS             12658..13602
                     /locus_tag="IWJ80_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9461599.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            13618..14397
                     /locus_tag="IWJ80_001888"
     CDS             13618..14397
                     /locus_tag="IWJ80_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP9461600.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            14410..15108
                     /locus_tag="IWJ80_001889"
     CDS             14410..15108
                     /locus_tag="IWJ80_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP9461601.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            15136..15900
                     /locus_tag="IWJ80_001890"
     CDS             15136..15900
                     /locus_tag="IWJ80_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP9461602.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYKQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEIKKKKFGLF"
     gene            15913..17730
                     /locus_tag="IWJ80_001891"
     CDS             15913..17730
                     /locus_tag="IWJ80_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9461603.1"
                     /translation="MFVLTRRRKIATLVVVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISTGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASDVSSILILLFINRQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIQVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAKGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNK"
     gene            17744..18967
                     /locus_tag="IWJ80_001892"
     CDS             17744..18967
                     /locus_tag="IWJ80_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HAP9461604.1"
                     /translation="MNKLEKRQIPFSPPDITDLEIKNVVEVLKSGWITTGPKTKKLEK
                     KITKWLGTSKVVCLNSQTAAAETGLRLLGIGENDEVIVPAYTYSASASVVCHIGANLV
                     LIDCQQDSLEMDYDALEAAITEKTKVIIPVDLGGIPCDYDRIFEIVEKKRYLFQPSNE
                     IQKAYGRIIVMADSAHAFGANWHDQMVGNVADLTTFSFHAVKNFTTAEGGALVWKEHD
                     KLDNEAMYHQCQLLSLHGQSKDALTKTQLGAWEYDIVGPWYKCNMTDVTASIGLAQFE
                     RYPNILKRRKNIIKRYDEAFQQLGIEVLMHYTDDYSSSGHLYITRVPNISSEQRNEII
                     IKMAEQGIACNVHYKPLPMFTAYKNMGFDINNYPNAYAHFVNEITLPLHTKLTDEEVE
                     YVINNYSKIVKDLVG"
     gene            18981..>19477
                     /locus_tag="IWJ80_001893"
     CDS             18981..>19477
                     /locus_tag="IWJ80_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9461605.1"
                     /translation="MRKWEDLPKEMQNDEIRYYYDILSKKKFSLVVKRVFDILVSATL
                     LLTLSWLFFILAIVIKIDSPGPVFYRQERITRYGKKFKIYKFRTMVINADKDGTLVTV
                     KNDDRITRVGKVIRKYRLDEISQLINVLVGDMSFVGTRPEVGKYVKSYSPLMLATLLL
                     PAGITS"
BASE COUNT         6646 a         3506 c         4066 g         5259 t
ORIGIN      
        1 tctttcttct agctgttttt ttcctgttta atatctctta tgttaaattg tgtttgattt
       61 ggttattctt ctggtaactc acttgcttct tgtttatatc ctgatacgcg atatacataa
      121 taaaagagca agctgccagc gtaaccaagc aatgttggga cgacccagcc aagacctaat
      181 gcagcaaaag gaacataatg atgataaaat cctagtaagc ttgctacgac agtttgctga
      241 ctcactaatg caggcgaatt tgccaacata tccaatacgg ctggaacggc agaaaagaac
      301 atcgttactt gatagacaaa tggactcttt tggaagaatt ttgctgttaa ggaaagtaag
      361 attaacgcaa gtgacaatgg atacaaaagc atcaatactg gtaaagacca ttggatgata
      421 ttatccaatc cggcattcgc aacgacaaaa gagacaaaag tcgttaaccg cagccatgtc
      481 atataactga ctttagggaa cagtttatgg aaatcttgtg caaaagaaac aacgagtccc
      541 atagcagtag tgaagacacc taatgtgacc attaccccta agaagctagc accgaagtta
      601 cctaaataaa aattagtgat ctgagaaagt gcagttccgc cattttctga taaagacatc
      661 ttattcaaac tcatcgcacc gagaaggacc agaccaaaat aaatcatgac ttctaacgca
      721 acgctcaggc taccagcttt tgctgttaat tctgtgactt ttttatcttt aaaaccaaga
      781 tcacgaactg catggacaaa agtgacactt aaagcaagta aagctactgc atcaaccgtg
      841 ttgtatcctt ctaaaacgcc atttgtcaaa gcgttcgtct gataagaatc tgcagcagat
      901 tggtttagat tccccatggg ttggaataaa gaaacgacaa aaatgatagc taataatgca
      961 agaaacaagg agtttaaata ctttccgaca tatttgacca aacgtgttgg atgtacagta
     1021 aggaaataag caagtaaaaa gaaaagagca gagaataaaa acatacctaa tgaatcatat
     1081 ttttcaggca aaaagggttt agcagccatt tcaaaagcag ttgctgctgt ccgcggtgta
     1141 ccaaagaatg gtccgatcgt taaatgaaca aggatcaaga acaaggcagc ataccatttt
     1201 ccgacaggtt tagctaagtc atacagacca tcactttttg tcactactac agctaagata
     1261 gctaataatg ggaaaagagc gcctgagatg gcaaatccta caccagcgct gatccagttt
     1321 gatccagcca tttgacctaa gtgtacagga aagatcagat tacctgaccc gaagaacatc
     1381 ccgaagataa gtgaactgac catcaataat tgcttccatg aataagtttt tgtttcttcc
     1441 ataaattttt cctccaataa ttatcatttt gagtaacaaa ataaacgttg cagaaactag
     1501 aaataccact aatatcagat tccatgaaaa ccaccacctt aaaatataaa aaaagccctt
     1561 ttccacttgg gaaaagggct ctagattcta gaacggtacc accttttttc gcttaaaaaa
     1621 gcttcttcat tcacggacgc ttttcgataa agttatccgc tagcctgata atggaggcag
     1681 ccacaatgat ctacgttttc gacattgact cggaaatgat gttcgtctat tcgatttatt
     1741 gtcatttctc acttacatga ctcactggga ataaatcact gaatagccta ctgttttcct
     1801 cgacgctttg ctgtatattt atttgttgac gttataatac acctgttttt tttgaaatgc
     1861 aagaatattt caaatatttt gttttctttt tttgaaacaa gaaaaatacc cagagaatac
     1921 ttgctaagtt agacggaata cttgtcctct atcttatgca tagcttacat gatcagcttt
     1981 ttttattcaa acgagacctt tagagaatgg aagcggctag ttttttaacg tttcttggta
     2041 cttatggtta agcaactctt ttagaacctt ttcttatttg ctatatagtt tcttctgagt
     2101 atggtaaaat aggaaagaat taatcgaaaa aaaggggatg tctatttttg acggaagagt
     2161 ctagttcttt agagattaga ttgacggctg ctgatgatgt cagcatgctt ttaggtgcac
     2221 acgacaagca tataaaactg attgaggaaa caacggaaac aaccatccat acacgtggag
     2281 aaatcattca gatcattggt gagcaatccc agatttcctt ggctgcctca gtcattcgga
     2341 cattacagga attgatcaaa cgaggaatcc acgtaagtac gcccgatgtt gtgacggcat
     2401 tgaaaatggg gcaaaaaggt acattagatt attttgttga gatgtatgag gaagagatcg
     2461 tcaaagaccg taatggaaaa cctatacggg taaaaaacag cggccaaaag aaatatgtcg
     2521 aatcggtagc caaacatgat atcgtatttg gcgtaggacc agccgggaca ggaaaaactt
     2581 ttcttgcagt agtattagca atcgctgcct tgaaaaaagg tgaagtgcaa aaaatcattt
     2641 tgacgcggcc tgctgtggaa gcaggtgaaa atctaggctt tcttcccggg gatctaaaag
     2701 aaaaagtgga tccttatctg cggcctgttt atgatgcttt ataccaaatt tttggattgg
     2761 atcatacgaa ccgattgatg gaacgaggcg taatcgaaat cgcaccttta gcttacatgc
     2821 gggggcgaac attggatgat gcattcgtta tcttggacga agcacaaaac acgacagtcg
     2881 ctcagatgaa aatgttcttg acccgtctcg gttatcaatc aaaaatgatt gtcaatgggg
     2941 atacaagtca gatagacttg cctcgaggga cgacaagtgg tcttgttcat gcagaacgaa
     3001 cattaaagca aatcaagaaa atcgattttg ttaattttga agcaagtgac gttgttcggc
     3061 accctgtagt tgccgaaatc atcaaagctt atgaaaaggc agatctccat caagaataag
     3121 ctttgcttag gaggataagc acatgttgaa taaaacactg cagaaactac aagaaaaact
     3181 tggaaaggca gcattgcctt tcctgctgtt cttgtttttt atcattatct gcggcgttac
     3241 atttagtagt gtcaaacaaa aggccaatga tttcagagaa ggccaagtag cagaagaaag
     3301 tatccgtgcg aacaaaacga tcgagaacac ggaagaaacc gaacaaaaaa gaaaactggc
     3361 agcagaagca gtcactccag aatacactta ccaaaaagat cttgcagatg accagaacaa
     3421 tcggatcaag caactatttg aattgatcga taagacgaat gatgccatca acaaatctta
     3481 tgacgaaaag attgataagg caaaggaaga tgaaaatatc ccaaagccta ctacagaaga
     3541 gcggatcgcc gcgctgaaga aaaattttga aaatgtgaat gcagaaaatg ttgtgtttta
     3601 tcaaaaacta ccgaacaatt tttattcgac gatatttgaa atgactgaaa ctgagattca
     3661 tactgtgagg gatgagagtc tgaagctcat tgacgaacag atgagcaaac aagttcgtga
     3721 aagtgaattg gaagctttta aacaagaagc agaagagcaa atccaatatt tgaatgtcac
     3781 accagcacaa caacaaatga ttcgctactt agtggatcaa ggtatcgtag tgaatgacgt
     3841 attgaatgag aaaaagacag aagaattgaa acaatccgca cgagaagctg tacaaccagt
     3901 gatgatcttc caaggagaaa tcattgttcg tgaaggtaat caaatcgatg cttcagctat
     3961 gaaaaaattg gaattactag gactgactaa tcaaacaact tcgatttttc cgctaattgc
     4021 catggtttta gctgttttgc ttcaaatcgg cgtactgatt tataattcac ttcaattcca
     4081 aaatgcagga gaaagaacaa aatacgtgct tttctatgtg acagctatgt cagtcagcat
     4141 agtgctgatg aaatttttcc aattattcca aacggaacaa gctacttata tcccgctgtt
     4201 ttatcccgct gcttttgcac cacttgtttt aagctttttc ttgaatcgaa gagcagggat
     4261 catggctggt attttccaag cagtatccgc acttttcatt ttttatgatt cgattggcac
     4321 gaactttcta acgattatct tgatgtctta tgttttctct gggctcatgg caacgattgt
     4381 cagacgcaaa cggatctcag aacaaggatt atctgctgcc atgtgggtca ttatttttcc
     4441 tgtgatgatg gatgtcattt tgatcgtcta tcaaggaatg agctttggtg atgcaacgac
     4501 ttggttaatg ctcgtctgcg ggttcactgg tgcactcttc tcattcttgc tgacgatcgg
     4561 tctacaccca tatattgaat tgatggtcaa tgatgacagt gtaattgtat taaatgaact
     4621 cagcaatcca aatcatcctt tattgaaaca gttgctggaa gaagcgccag gtacttatca
     4681 tcatagtatg atggtagcta atctaagtgc taacgctgta gcagaaatcg gcggacgttc
     4741 tttgctaact cgagtagctt gctattatca tgatatcggc aagatcaaac atgctagctt
     4801 tttcgttgaa aacttgcctt caggagcgga aaacccgcac aatttcttat tgccagaaga
     4861 cagtaaacat attatctttg gacatgtgac agatggagcc aaaatattgg aagattataa
     4921 tatgccgcaa atggtgatcg atatttgcaa acagcatcat ggaaccacgc taatgcgtta
     4981 tttctacatc aaagcgaaag aacgcaatcc ggaagtaaca gaagagcaat tccgttatcc
     5041 tggaccaaaa ccgcaaagtc gtgaagcagg gatcgtcaat attgctgaca gttgtgaggc
     5101 tgcagtacgg gcaatggatc atccgacgag cgaaaaaatc gaaagttttg tccataatct
     5161 tatcgaagag cgtatttccg atggtcaatt agacgatagt ggactgacct tgaaagaaat
     5221 ccgaaaagtg gaaaaatcat taatcagcgg tttaagtagc accttccatt ctcggatcaa
     5281 atatcctaaa atgaaaacag aagttgagaa gataaaggaa gtacaagaag aatagaaaga
     5341 agcgatgtat aatggatatt acctttatgg atgaaacaaa ccaagtctca gaagagaaaa
     5401 tgaaagaaat cgatgacttg ctgcaatttg cagcatctta tttaaagctg ccagaggaaa
     5461 cagaaatgtc tgttactttc atggacaatg cggcaattca agtaatcaat cgtgattacc
     5521 gtggaaaaga tgcaccgaca gacgtcatca gttttgctct ggaagaagaa ggcgaagacg
     5581 aaatcccagt gatttttgaa gatggagaga atccattacc tcgtgtacta ggagacatca
     5641 tgatctctat tgaacgtgct gctgaacaag cagaagctta cggccattca tatgatcggg
     5701 aacttggctt tttagcggta catggatttt tgcatatcaa cgggtatgac catatgacac
     5761 cagaagatga gaaagaaatg tttggtttac agaaagagat cttggatgcc tatggactca
     5821 aaagataaaa acgtagagaa aaacaaacat ttcctgatgt ctgtggaatt tgctgtccaa
     5881 ggagtcaaaa ccgtttttga ggaagaacgc aatatgagga aacatgttgc gttcggtatc
     5941 cttgctttat ttatgggcgt gattttccag cttgatcgga tagagtggtt atggctgctt
     6001 ttagctgttt ttctcgtttg gatagtagag atcatcaata ctgtttttga aaatgtagtg
     6061 gacatgttta cggattttca ttttcatccg attgggaaaa aaatcaaaga tatggcagct
     6121 ggagcagttt tagtgaccgc ttgttttgct gtcatcattg ggttgatttt atttgtccct
     6181 aaaatatatc atttgttgtt taattaaaaa ggagaacgta tatgccagaa cataaatcag
     6241 ggtttgtcgc aatcgtcggc cgcccgaatg taggaaaatc cacattattg aaccgaatcg
     6301 tgggtcaaaa aattgccatt atgagtgaca aggcacagac aactagaaat aaaatccaag
     6361 gagtatatac tacaccagaa gctcagctga tttttatcga tacaccagga atccataaac
     6421 caaagcatcg tctaggagat tttatggtag aagcagctta cagtgcgttg aaagaagtgg
     6481 atgcagtctt atttatgatc agtgctgatc aaaaacgtgg ccggggagac gattttatta
     6541 ttgaacgtct aaaaaacgtg cattctcctg tttatctgat tatcaataaa atcgataaga
     6601 tccatccaga cgagttgctg ggaatcatcg aagattattc gacgcagatg ccttttgcac
     6661 aagtgattcc gatctctgct acagaaggaa acaatgtcga acgcttaatg gatgtattag
     6721 tcagtgaaat gccagagggc ccacaatatt ttccagatga tcaagtgact gatcatcctg
     6781 aatacttcat cgtttctgaa ttgatccgtg agaaagtctt gtttttaaca cgagacgaag
     6841 tgcctcattc tgtcgcagtc gttgttgatt cgatgaagcg aaacgagaat gataaaatcc
     6901 atatccaggc cacgatcatc gtcgaaagag atagccaaaa aggaatcatt atcggcaaag
     6961 gcggaaaaat gctgaagcaa gtaggaacga aagcaagaaa ggatatcgaa aatcttttag
     7021 gagacaaagt atttctagaa ctttgggtca aagtccaaaa agactggcga gacaaaaaag
     7081 tctatctgca agacttcggc taccgaaaag acgagtactg atattctgga cttcttatag
     7141 gttaggtata gaaataaact ggataaaaat ccgtacgttg agaaaaacag cggaagatcg
     7201 aaacataaat aatctggatc ggcagaatct gcttttccag atttattttt taggagggag
     7261 aatatgagcc agcttgtaga atcgaaaggc ttgattttat ctagccgtaa ttacaaagaa
     7321 aaagataaat tagtcaaaat ctttacggaa tcttctggca aaatgatgtt ttatatcaaa
     7381 ggaatccatc ggcaaaatca gccattagct ccggctttgc tcccctttac cgaagcagtc
     7441 tatatcggta attttcgaag tgaaggtttg tcgtttttaa gcagtgtcaa agacgtccat
     7501 gcgtttcgga acatccaaga agatatcttt ttgagcggtt atggtacgta tttgctaaat
     7561 ctagtagatg cagcaatcga agatcatcaa tatgacccgc atttattcca atttacgcaa
     7621 caagctctgc agcggatgga ccaaggagat gatgcagaaa tcatcacgaa tatctttgaa
     7681 gtgcaaatac tgcaacgctt cggaatcgcg ccaatttgga cgcactgtgt cgtatgcggg
     7741 gaaacaaaag gaaaatttga ctattcttcc aaatatggcg gtgtgatatg cgagaaacac
     7801 tggccaatgg acgaacaccg ttatcatgca gatcctcgcg ctgtttattt cgttcgaatg
     7861 ttttcggcta tttcttatga taagatcagt ggaatccact tgaaagaaga aacgaagcag
     7921 gcaatccgtg acctcattga ccagttgtat gatgaatatg tcggtattca tctaaaaagc
     7981 aaaaagttca ttgatcaaat gaagtcttgg gcacacgtat taaagacgcc agagaaaaat
     8041 atgcctgaaa aagagatacc tgaaaaagaa gaataaatga aagatacaac aaaaaaatca
     8101 aaactatttg acaaatacag ggacttccgt ctattattaa ccgtagatta aatagatcgt
     8161 tcactgagag gatctattat acaaaccaga aaactgcaaa atgaatacgt acattgacca
     8221 agatcagtaa aaaacacgtg gtttagcgag ttcgggagag tggaagccgg acccaaacga
     8281 tttttgaaga ggcacttgcg agtacgataa atgaagctgc cgaatcttta ttcggaagta
     8341 gggtggaacc gcgaaattat tcgtccctat gtctgtaatc agacataggg actttttgtc
     8401 gttttctttc ctgtattctt attgacagca acgcttaaaa attgaaaggg gcaaaacaga
     8461 tgagtaaaaa attgaccgtc caagatatga ttttaacatt acaaaaattt tggtcatcaa
     8521 acggctgcat gctgatgcaa gcttatgata cagaaaaagg agcaggtacg atgagcccat
     8581 atacattctt acgggcaatc ggaccggaac catggaatgc tgcttatgta gagccatccc
     8641 gccgaccagc tgacgggcgt tatggagaaa acccaaaccg tttgtatcag catcatcaat
     8701 ttcaagtcgt aatgaaacct tcacctgaaa atatccaaga gctttattta gaaagcttgc
     8761 gtcttttagg aattgatcca ttagaacacg atattcgctt tgttgaagac aactgggaga
     8821 acccatcgat gggttgtgcc ggtgtgggct gggaagtatg gcttgatggg atggagatca
     8881 cacaattcac gtacttccag caagtcggag gactaccttg taaaccagtt acttctgaga
     8941 tcacttatgg actagaacgt cttgcttctt atatccaaga agttgaaagt gtttatgatc
     9001 tggaatgggc tgatggcgtg aaatatggtg agatcttcaa acaaccagaa tatgaacatt
     9061 ctaaatattc ttttgaagta agcgaccaag acctgctact aagcaatttt gatcgttttg
     9121 aaaaagaagc taaacgctgt atcgatgaaa atcttgttca tccagcgtat gattatattt
     9181 tgaaatgtag ccatacattc aatttattag atgctcgcgg tgcagtatcc gtaacagagc
     9241 gtgcaggtta tctttcccgt atccgcaaca tggctcgtgc tgtagccaaa atctttgtgg
     9301 cagaacgcga aaaattagga ttccctttat tgaataaaaa cacagcaaat ccagaaaacg
     9361 tgaaggaggc tgaataagat ggcaaaagac ttattacttg aaatcgggct ggaagaaatg
     9421 cctgctcatg tagtcacacc aagcatgaaa caattagaag aaaaaacagc aaaattttta
     9481 gatgaaaacc aattgactta tgatacgatc gaaacgtttt ctacaccgcg ccgcttagca
     9541 atcaaagtaa caaacattcc agaacgtcaa gcagacagcg aagaagaagt caaaggacca
     9601 gccaaaaaaa ttgctttgga cgcagatgga aactggacga aagcagctga aggatttgtc
     9661 cgcggacaag gtctgacgac agatgctatc acttttagag aattaaaagg cgtggaatat
     9721 gtctatgtaa cgaaatacac tcacggcaaa gaagcaaaag aagtattgac aggtttagca
     9781 gatgtcatta aaagcctcac tttcccagta acgatgcact gggcagaata cgattttgaa
     9841 tatattcgcc ctattcactg gatcgtggca ttgttagatg acgaaataat cccgtttgag
     9901 atcttggatg tcaaaacaga cagagtttca agaggtcatc gtttcttagg cgaagatgtg
     9961 acatttacgc atgcaaaaga atacgaagaa aaattgaatg aacaatttgt tattgcttca
    10021 ccgcaaaaaa gaaaagaaat gatcatcaca caagcaaacg aattagcaga aaaaaatcac
    10081 tggatcattg atctagatga agatttacta gaagaagtca acaatctagt cgaatatcca
    10141 actgtctttt ctggcgactt cgaagaaaaa tatctatctg ttcctgaaga agtactagtc
    10201 acttctatga aagaacatca acgttatttc gaagtacgga acgatcaagg tatgttgctt
    10261 cctcatttta tttctgctcg taatggcgat cgggtaaaat tggaaaatgt ggtacgaggc
    10321 aatcaaaaag tattgactgc tcgtttagaa gatgcggaat tcttttataa tgaagacaag
    10381 aaagcttcaa tcgaccaata cgttgaaaaa ttaaaaactg ttactttcca tgaaaaaatc
    10441 ggtagcatct atgagaaaat gcaacgggta caagtcatcg ctcgcatcat aggcgaacaa
    10501 gtaggactat ctgaggaaga attgatggat ctgcaacgcg ctgctgaaat ctataaattt
    10561 gatctagtaa ccaacatggt aggagaattc cctgaactac aagggatcat gggagaaaaa
    10621 tatgctctgc ttaaaggcga gaaaccagcc gtagcaacag cgatccgtga acattacatg
    10681 cctatatcca gtgaaggaga tttacctgag actacagtag gttctgtttt agcgattgct
    10741 gataaattag atagtatctt ttctttcttt gcagttggga tgatcccaag tggctcaaac
    10801 gatccatatg ctttgcgccg tcaagcatat ggtgtcgttc gaatcatcga atcgaaaaaa
    10861 tgggcctttc ctttgtctgt tctgcaagaa acgattagtg aagttatcag taaagatacg
    10921 gatcgttttg gcattggctt gtcagcggga caacaacagg tgatcgactt tatcaaaggc
    10981 cgtctgcgcc aattattgac aacgaagaat atccgtcatg atgtcatcga agctgttttg
    11041 aatgcagaac aaaaagatct gacaaaagta tttgcagcag cacaattgtt caaacaacat
    11101 cttgcagatg aagacttcaa accttcaatg gaagcattga cacgtgtcgt gaacttagcg
    11161 aaaaaagcgc agcttgaaaa acaatcagaa gtagaccctg agctgttcga aaacgacgca
    11221 gagaaagaat tacacaaagc agtagaagca atgaaagcaa gttttgctga ccaaacgatc
    11281 cacgaaaact acgaccagct tgtcgctctt cgtccactga tcgaaaatta ttttgatcaa
    11341 acgatggtca tggctgatga accagcagta cgtaagaacc gactaacaca acttagtcaa
    11401 ataactgcca tggctttgtc attagcaagc ttggatcaaa tcatcacaaa atagtctttt
    11461 gtttgaaaaa cagagagcat gcacgaaacg tgctgtgtct ctgttttttt gttataaata
    11521 taaaagctgt tattaattct attttaataa tttttgttat tttattcttt gctagaattt
    11581 tcaaaacgct catgaaaagt gaggaaataa aaatgaaaaa gattaataat aatcttgtta
    11641 ctgttttatt tacttttaaa ttaggaattt ccctagtcaa gaaaactgtt tgtggtatga
    11701 ttatataggg aaaacctgtt tataaatatt tctatgaata tattagttta attatgcttt
    11761 taaaataaaa agcgctcatt ctgatgtgaa tgaatcacat ctttatttat ctatcatcaa
    11821 tatttaggag tgaatctatg gaaaagccgt tcgtcaagtt attggctact gtggcggtag
    11881 gcacaggcgc tattgtgatt tgcttgtttg gataccatct caataatcag cggcaacatc
    11941 atcaacggat caattacgca gaatctgcca tcaccaatca aaaagacacc gtgacctctt
    12001 tatcaaaaga agtagataag ctgtacagta caaaagaaaa aattttccta aatcctgaaa
    12061 taacagaaga aacagtttca aatttgtcac ataaactatc ttctatcaaa ctttcagcag
    12121 atgattatga catcaaggaa tcagaattac ctaaagaagc tgctgcgatc caagaagaaa
    12181 agaaagcagt cttaacacag ttagaggatg cagaatcaaa actgaagatc caaacggctg
    12241 tcaataaatt atttactaaa aacgtatcaa actggcagca agctgtggat gatgtcatca
    12301 tcaaagaaaa gttggcaagt gcagatgtcg cccatgtacg tgaaaatatg agctttttta
    12361 aggatagtgc ttggaaaacg gtcgttatgc aatatctagg attcgctgat acccaaattg
    12421 cacaagtcac gcaactagat cagctattcg atacgatgct gaaagacggt caagtaacag
    12481 ctactgctac ttacgatcaa tatttgaccg cactcagtct catcgaacag atccggaatg
    12541 aaaaaataag agccgcatac gcgtcaaaag cagaaacagt cgctcagcaa atgggctata
    12601 gcaatacgag ctattaataa gcgagaattc aagcaaggct agcaaacgga ggaaactatg
    12661 gcaaaacgaa gaagaagatc aagagggaaa aagatatttc taattatttt agcaattctt
    12721 gtcgtattag tattagcaat cgtaggagta ggagcaaaat tatatctaga tctctcacat
    12781 tctattgaaa aaacttacga atcagtggaa cgaacccaag gagaacgaac aaaaccagtt
    12841 gatctggaca aacaagaatc tttctctgtt ctattgatgg ggattgatac gggagattta
    12901 ggtcgggtcg atcaaggaag atcagatacg atgatggttg ccacagtcag tcctgaagac
    12961 aaacagacaa cgattgtcag cattcctcgt gatacgtatg tcgacatcgt tggacatgac
    13021 acaaaagaca agatcaacca cgcttacgca ttcggcggag ctgccatggc gatggatacc
    13081 gtacaaaaat atgtagacat cccaatcgat cactatgttt caatcaatat gaaaggtcta
    13141 aaagaactcg ttgatgcagt tggtggtatc gaagtcaata atgacctcac cttctcacaa
    13201 gatggctatg attttacgat cggtaaaatc acattgaacg gggatcaggc attgtcttat
    13261 tctcgtatgc gttatgaaga tccaaatggt gactacggca gacaagaacg tcaaagaaag
    13321 atcgtagaag gtgtagcgaa aaaagtgttg agtctgaacg gtgtaagcaa ctatcaatcc
    13381 gttttgaatg ctctagagtc gaatatgaaa acagacatga gctttgatga aatgaaaaag
    13441 attgctttag attatcggga tgcatttggt acgatcaagc aagatcagct gcaaggagaa
    13501 ggcttcatgc aagacaatat ctcataccaa aaagtcagcg atcaagaact tgatcgtgtg
    13561 aaaaaagaat taaaagatca aatgaatcta gagaataaat aggaaagtgg agaaaaaatg
    13621 gaagagacaa tcagcttgaa agaaattttt gaaacattac gcaaacacgt gataacaatc
    13681 ctcatctcaa tgtttgctgg cttggccatc tcaggcatcg tgacgttttt catcttgacg
    13741 ccaaaataca gctcacaagc ccaattgatc gtgacactgc cgcaatcaga tactgcaaat
    13801 gtcaatgatg tcaatacgaa cctgcaaatg atcaatacgt ataaggatat gatcgtcagt
    13861 gatctagtac taaatgaagt caaagatcgt ttagaaacag aagacaatgt aaaaatgact
    13921 gctggacaaa tcaaagatgc catctccgtc aatcagtcac aaaattcaca aatgttctcg
    13981 atccaagcaa caagcacaaa tgcagtgacg gctcaacaaa tcgctaatac aacgtcacaa
    14041 gtattccaag agaacgccaa agacgtgatg aatgtcgata aaatctcgat catctcaggt
    14101 gcagttgcca atacgacacc tgtttcgcca aataataaat tgaatctagt gattggctta
    14161 gtgttaggaa tgatggtagg cgtaggtcta gctttcttat ttgaattact cgaccggaca
    14221 gtaaaagatg aaaaatatgt gacggataca ttgggcttcc cgatcttagg gactgtacca
    14281 gaaatgtcag cgaaagaatt gaatgctggt atccgtaaaa aaggaccagc tccgacacaa
    14341 ccaaaagcta caaacgataa aacagcgcca tcacgcagaa gccgctcaag agtctaggag
    14401 gaaagaaaaa tggcacgaac acagaaacaa aaagcagccc aaactcatgc agtcagtttg
    14461 gtcacactca caaacccctc ttcaccaatc gctgaacaat atcgtacgat tcggacgaat
    14521 atccagtttg cctccgcagc tggtcagcaa attaaaacga ttgttgtgac ttcatcagga
    14581 cctggagaag ggaaatcgac cactgcagca aacattgccg ttgtttttgc caaatctggt
    14641 caacgggtgc tgctagtcga tgcagactta cgtaagcccg tcatctataa aacgttcaag
    14701 ttgaacaacg caagcggatt aagtaccgtt cttagttcat caggaagtgt agcagatgag
    14761 atccaacgaa caccagtcga gaatttgtcg attctgccaa gtggacctaa accaccaaat
    14821 ccatcagaac tactaagttc gccaagaatg gatcagattt tagcagaagc aagacaacta
    14881 tttgatgtag tgatcttcga catgccgcca gtagttgccg taacagatgc acagatcatg
    14941 tcttccaaaa cggatggcac cttattggtc gtacgtgaaa atatgtcacg gaaagaatca
    15001 ttgactaaag caaaagaatt gctggatatg gtacaagcgc gagtccttgg tgttgtatac
    15061 aatggggcag aacacagcaa agatgctggc tattactacg ggaattaaga aataaaaaga
    15121 gaggagacaa gagaaatgat cgatctacat tgccatatcc tgccaggaat cgatgacgga
    15181 gcagaaaaca tagatgccag tatcgccatg gctgaaaaag cgattcaaca agggatcaca
    15241 catattttat gcacacccca tcataataat ggaaaataca gcaatgaaaa atcacaagtc
    15301 atttctctag tcgcttcgtt acaagcagaa ttagagaaaa gacaattgcc tttgacgtta
    15361 ttggaaggac aagaagtacg tattaccgga acactgatcg aagacatcga ccgagatgaa
    15421 attttattta cagatttaga tgatacatat cttctaatcg aatttccgac actggaagtg
    15481 ccgttatatg cagaacagct cttttttgag ttgttacaac taggaaagac accagtgatc
    15541 gtccatccag aacgcaacgc gcattttcgt aaagacccga atcatatgct gccattccta
    15601 gatatgggat gcttggccca actcaccgca ccaagttatg tcggtgtatt tggaaaagac
    15661 atccagaaaa cagccaaaat gatggtcaaa cataatcttg tgcagatggc tgcttctgat
    15721 gcccatgggg tctctaagcg caccttttac ttaaaagagt gctataagca gattgccaag
    15781 gactttggta aggaaaaagt agtacagatg aaacaagtcg caaaggatct gatcaacggg
    15841 gatgaaatca gttatccagt ctatgaagag ataaaaaaga agaagtttgg tttattttag
    15901 ggggagtgta aggtgtttgt attaacaaga agaagaaaaa ttgccacatt ggttgttgtg
    15961 gacagtttgt tattgatcgc tgcaaatatt gcagcagcta aattcatgaa gccgtttgta
    16021 gctataccaa tggaccttat tttaatctct accggtttgt cgattggttt ttatttattt
    16081 tatggtagtt tattcaaagt tttcactcgg atcaatcgct atacaaattt aagagagatc
    16141 attgcaattt ttggttcact atctgcaagt gacgtctcaa gtatcctcat actgctgttc
    16201 attaatcggc aatacagttt acggttagtt atctttgctt atctgctttc gctcttattg
    16261 atcatcggta gccgattgat ctggcgtatc tacatcgaaa caaaaaatat gagatatgta
    16321 tcagcggatt ctgctaaaaa tacattgatc gttggtgctg gcgaaggcgg gcgtatttta
    16381 tacaacagct ttctgggatc caaaacggct caagatatcc aagttgtcgg atttgtagat
    16441 gatgatccga ataaaagaaa tacgtatctt tcaggaaaaa aagtattagg tgcattaaaa
    16501 gatattccgg aattgattga aaaatatgac attcaaatgg taacgatcgc tatcccttcc
    16561 ttatcaagaa aaaaattacg aagaatcttc gagttagtcg aatccgctca cgtgaaagtc
    16621 aacacgatgc cttctataga agaacttgct tcaggaaaaa ttagcgtatc aaaattaaaa
    16681 acaattgacg tagtggattt actgggaaga gatgaggtaa agctggatat tgaatccatc
    16741 aaagatcaga tcacagacaa agtcattctt gtcaccggcg caggaggatc gatcggttca
    16801 gaaatctgtc ggcaaatcat tcaattcaat cctgcaaagc tattgctttt aggacatggg
    16861 gagaactcga tctacttgat tgaccgtgag ttacgcacgc atcatcaaaa ttgtcctaca
    16921 gaaatcgtac cgatcatcgc agatatccaa gatcgcgaga aaatcaatga gatcatggaa
    16981 caatatcatc cagatatcgt ctaccatgca gctgctcata aacatgtacc actgatggaa
    17041 tataatccaa aagaagcagt caaaaacaat attttcggta caaaaaatgt agcagaagca
    17101 gctaaagcag caaaagtgaa aaacttcgtc atggtatcga cagataaagc taacaatcct
    17161 ccaaatgtca tgggttcaac aaaacgaatc gctgaaatga tcgtgactgg attgaatgaa
    17221 gaaggctgta cgaaattctc agctgtcaga tttgggaatg tccttggttc tcgcggtagt
    17281 gtgattccag tattcagaga acaaattgct aaaggtggtc caatcactgt aacagacttt
    17341 cgtatgaccc gatacttcat gacgattcct gaagctagtc gtttagttat tcaatctggc
    17401 gcattagcaa aaggtggcga aatttttatt ctagatatga gtgaaccagt gaagattgta
    17461 gacctagcca aaaacatgat ccgattgagc gggtatagcg aagacgaaat cgaaatcatt
    17521 gaaacaggta tccgtccagg ggaaaaacta tatgaagaac tactgttgga taaggaaaga
    17581 aatgatgaag cagtttatga gaaaatcttt gtaggaaaca tcaaaggtta ctcaatacaa
    17641 aaagtgatgg attttgtcaa gagcctgccg caagacgatg aacaattggc taaagatatc
    17701 gtcacttttg caaatgcttc gaataaatag gggggaaata taaatgaata aattagaaaa
    17761 acgtcagata ccattttcac cgccagatat tactgattta gaaataaaaa acgttgtaga
    17821 agtattaaaa tctggatgga taactacggg accaaagaca aaaaaattag aaaaaaaaat
    17881 tacaaagtgg ttaggaactt ctaaagtagt ttgtttaaat tcacaaactg ctgctgcaga
    17941 aacagggtta cgtttgctgg gaataggaga aaatgatgag gttatcgtcc cagcttatac
    18001 ttattctgcc agtgcatcgg ttgtatgcca cataggcgcg aacctagtat taatagactg
    18061 tcaacaagat tctttggaga tggattacga tgcattagaa gcagcaatta cggagaagac
    18121 aaaagtgatt attcctgttg atcttggtgg tataccttgt gactacgacc gtatttttga
    18181 gattgtggag aaaaaaagat atttatttca acctagtaat gagattcaga aagcttatgg
    18241 tcgtataatt gttatggcag attctgctca tgcatttgga gctaattggc atgatcagat
    18301 ggttggtaat gttgctgact tgacaacatt ttcatttcat gctgttaaga attttacaac
    18361 tgcagaaggt ggagctttag tatggaaaga gcacgacaaa ctagataatg aggcaatgta
    18421 tcatcagtgc cagctgcttt ctttgcatgg acagtcaaaa gatgcactta ctaaaacaca
    18481 gttgggtgca tgggaatatg atattgtggg tccatggtat aaatgtaata tgactgatgt
    18541 tactgcatca attgggttgg cacaatttga acgctatcct aatattctga aaagaagaaa
    18601 gaatataatt aagaggtatg atgaagcctt tcaacaacta ggaattgaag ttcttatgca
    18661 ttatacggat gattatagtt cttcaggcca tttatatatt actagagtac ctaacattag
    18721 ttctgagcaa agaaatgaaa taattataaa aatggcagaa caagggattg cttgcaacgt
    18781 tcactataaa ccacttccaa tgtttacagc atataaaaat atggggtttg atattaataa
    18841 ctatcctaat gcctatgctc attttgtaaa tgagattaca ttaccattgc acacgaaact
    18901 tacagatgaa gaagtagaat acgtaattaa taactatagt aagattgtaa aggatttggt
    18961 tggatgatag gagaggcaaa atgagaaagt gggaagattt gccaaaagaa atgcagaatg
    19021 atgaaatacg gtattattat gatatcctca gtaaaaaaaa attcagctta gtggtaaaaa
    19081 gagtatttga tattttagtg tctgcaactt tgttactgac actttcatgg ttatttttta
    19141 ttcttgcaat agtgataaaa attgattctc ctggacctgt attttataga caagaacgta
    19201 ttactcgtta tggaaaaaaa tttaaaattt ataagtttcg tactatggta ataaatgcag
    19261 ataaagatgg cactcttgtt actgtgaaaa atgatgatcg tattacaaga gtaggaaagg
    19321 taatccgtaa atatagatta gatgaaatta gtcagctaat taacgttttg gtcggagata
    19381 tgtcttttgt aggaaccaga ccagaagttg gaaaatatgt aaagtcgtat tcaccattaa
    19441 tgttagccac attattatta ccagccggga ttacatc
//
LOCUS       DACDRN010000046        17587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDRN010000046 DACDRN010000000
VERSION     DACDRN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.046"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            30..995
                     /locus_tag="IWJ80_001894"
     CDS             30..995
                     /locus_tag="IWJ80_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9461606.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(1094..2266)
                     /locus_tag="IWJ80_001895"
     CDS             complement(1094..2266)
                     /locus_tag="IWJ80_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9461607.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(2253..3398)
                     /locus_tag="IWJ80_001896"
     CDS             complement(2253..3398)
                     /locus_tag="IWJ80_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9461608.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(3412..4344)
                     /locus_tag="IWJ80_001897"
     CDS             complement(3412..4344)
                     /locus_tag="IWJ80_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9461609.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            4461..5540
                     /locus_tag="IWJ80_001898"
     CDS             4461..5540
                     /locus_tag="IWJ80_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9461610.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            5537..6166
                     /locus_tag="IWJ80_001899"
     CDS             5537..6166
                     /locus_tag="IWJ80_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9461611.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(6276..6503)
                     /locus_tag="IWJ80_001900"
     CDS             complement(6276..6503)
                     /locus_tag="IWJ80_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9461612.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(6606..9194)
                     /locus_tag="IWJ80_001901"
     CDS             complement(6606..9194)
                     /locus_tag="IWJ80_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP9461613.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(9214..9873)
                     /locus_tag="IWJ80_001902"
     CDS             complement(9214..9873)
                     /locus_tag="IWJ80_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9461614.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(9922..10425)
                     /gene="trmL"
                     /locus_tag="IWJ80_001903"
     CDS             complement(9922..10425)
                     /gene="trmL"
                     /locus_tag="IWJ80_001903"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP9461615.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(10646..11512)
                     /locus_tag="IWJ80_001904"
     CDS             complement(10646..11512)
                     /locus_tag="IWJ80_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9461616.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            11621..12262
                     /locus_tag="IWJ80_001905"
     CDS             11621..12262
                     /locus_tag="IWJ80_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP9461617.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(12324..13685)
                     /gene="mgtE"
                     /locus_tag="IWJ80_001906"
     CDS             complement(12324..13685)
                     /gene="mgtE"
                     /locus_tag="IWJ80_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP9461618.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPLKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(13703..14602)
                     /locus_tag="IWJ80_001907"
     CDS             complement(13703..14602)
                     /locus_tag="IWJ80_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9461619.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(14604..15401)
                     /locus_tag="IWJ80_001908"
     CDS             complement(14604..15401)
                     /locus_tag="IWJ80_001908"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP9461620.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(15379..16083)
                     /locus_tag="IWJ80_001909"
     CDS             complement(15379..16083)
                     /locus_tag="IWJ80_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP9461621.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            16214..16789
                     /locus_tag="IWJ80_001910"
     CDS             16214..16789
                     /locus_tag="IWJ80_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP9461622.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            16907..17584
                     /locus_tag="IWJ80_001911"
     CDS             16907..17584
                     /locus_tag="IWJ80_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9461623.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         5197 a         3783 c         3133 g         5474 t
ORIGIN      
        1 cttgttttat tgaatagcag gaggaaatca tgaaattcca ttatcatata ttgatcaatc
       61 catcggcagg cagcggaaat ggctataaag tagcagaaag aattttaccg gtattaaaaa
      121 ataagcatat tgactataca cttcattata gtgaatacaa aggacacgat gctgagattg
      181 ctgaaacttt ggcaaaagaa acattgattc cttggataga agaagaacag aaaacaatcg
      241 atactttccc ccttttgatc atcgtaggag gtgacggcac cttacatcag gtacttgata
      301 ctttctatca gatggaagta gaattcccag ttgcttatat tccagcaggt tctggaaatg
      361 attttgctcg aggggcaggc cttccaaaaa atcccaaaaa aggacttcag ctgattttag
      421 cagctcaatc accagaaaaa gtccacataa tggcttatga agaaaaaata agtgaaaaaa
      481 aaggaatagc tgttaataat ttcggtatag gattagatgc agctatcgtc catgcgacaa
      541 atcattcttc cacaaaaaag cgattgaaca aatacaactt aggatcattt tcgtatttat
      601 tctctattct ccgtgcttta ttcactcaaa aaggatttcc aattctggta gatgcaggag
      661 gaaaacaatt aagtttttca aacgcatttt tatgtacagc aacgaagcat cctttttttg
      721 gcggaggaat cgcgattgtt ccaactgctg atgcgtctaa accagtcatt gattttgtcg
      781 tggtcgaacg aatcaatttc tttaaaatct tgtggctcat tcttttactt atacagaaaa
      841 aacaattgaa atcgaaatat ttccatcact acacaacaaa cagattacgt atcgtttcaa
      901 caacaccgca acatggtcaa gaagatggag aagaaatgga aaaacgacct tttgatatca
      961 caataaccac aactaaacag ttgttttggt gttaataaaa gaggttttgt cagaagctac
     1021 atctggaaca ccgtttattc ggaaaaaagg actgtgacac gacttttgtc acagtccttt
     1081 ttttactatg cagttaattc tttgcccaat tgggttgcca tcgatagttt ctttctttac
     1141 ccaaaataaa ttggatcatt gaaaacaccg cagcaaaagc aactaagaca caaccattga
     1201 accaaaaaga ttctgcaaat gtttgattgt aactgagtgt ttcactgatc aagttattat
     1261 tttgaacaaa ccaatatgtt ggtgtgaatg ccgctatttt gctaaccatt tctggcaagt
     1321 attctgctgg tacaaacacc ccaccgataa agcaactgcc tgtgataaaa atatctttga
     1381 tcccaccgct ggtttctgag ttttttacca atgaagtgat acaagccgaa aagctaatga
     1441 tcggcaataa aaacaagaaa gtatttaaaa tatgatacca taccgttata tcccacgagg
     1501 tgtggatcac taacagaacc attagaagta agaagccact gaacaacagc agcgagtaac
     1561 taatcaaact aaaggttact ttgcgattca agtttcgttt ggtaatgggc gcactctggt
     1621 ttcgtttact gatctctttg cgattaaacg ctagattgat gacgctgaaa ccgctaaaaa
     1681 tggatagaaa caacccataa gacaagaaat taaaggttgt gcccgtcaac agctttttca
     1741 tccgctgact atatcccgca cttagctcaa cttctccagt aaccgagagt gccatttgtg
     1801 tctgttccat agctgcatcg attgttttat cttcttcttg aaacagaatc agtgtttgca
     1861 aaaaggtgtt cacttgctgt gtaacaactg ttttagaaaa ggcatctggg cgactctgta
     1921 tcttgatcgt cgccatcttt cctacttcga cttgttcggt aaagtctgca ggcagatgta
     1981 agataatgtt tacacgatta aaataaagag catcatcgat ttctttttgt gaggttccat
     2041 ccaatgtaac gactgtttcc ttgtcattca aatatttacg aaggccatcg actacaggcg
     2101 actcttcttc actaatcaag gcgattttcg catcttgcag agaggtattt tcaacatcaa
     2161 tattttgcgc aaaaaagaat gtggcaacaa cagtgataat tacacctatc agtaaattcg
     2221 atttatttgc tttaagcaat gtccaaatcg ttttataaag cgtcataacg cacccctctt
     2281 tccatccaag catttcctaa cgcaaaaagg aaaataaatc cgatcaacca tagaagattg
     2341 gtataaaaac tagccaaatc agtgtagaaa taaagtttat acaaactttc gctgatcaag
     2401 ttgacgagat ttaattgacc aacaatcgga atatgaagat caatccaata cttcacttgt
     2461 tccgacccca tcataccagc aaaaaagctc atcgcaatcg tcactgaaat caagatgcta
     2521 tccttttgca ccagattcat ttttggtatc aaattaccaa ccaatgtacc taacaaaatc
     2581 gcacacaacg accccagagc ggtaacaatc atcaaccatt gccaacgttc accaaagtct
     2641 acttgataga taaaacgaaa gacagctaac ataatataaa tctctgtcaa aacaatcgta
     2701 aagcttgctg ccaaattagc gattgaaact aacgattgct ttctaggaat caaggacaag
     2761 cgaataccat ttgacgattg atttgcttgc tgatcctgta cattgcggac cccccacatc
     2821 gtcccgtaga gaatcgccat tccaatcaga gtgaagaaat aaaaactttt gaaactcata
     2881 ttacgacctg catgaacttc ttgaaaagat acttcttgtt caaaaacagt catgatgggc
     2941 gcgagatcaa cgttctccac ttctgccact ttactcgaaa ttataccaat acgctgtaaa
     3001 aattgatcca tgacgttttt catcaacgtc tgactcattc ctgcttgacc cacatggagc
     3061 gaaacagatg aaccatcaat gagataaaaa ccagccaaat catctgcatt tagcttttca
     3121 ttcgcttcta cttccgatag ttcctgtact tcaaataatg gtttatcatt gacttcgact
     3181 tgttctaaga ctgtggtaaa attttccgaa agcgctgttt gatccgcttt gattacaatt
     3241 ccagtattaa tcgtttcaag attgccacct tgatcaatct cgccaaaggc cgccataaac
     3301 atcaatccta aaagaattgg aaaggccaat gtccaaaaca acatggattt atttcgcagc
     3361 agtatttttg tgcgatattt ataaaaatgc caaaacatta tttcccctcc tctagtctct
     3421 caaatccttg ccagtaatct ctaaaaagac atcattcaag gacgcttctt tggttgctat
     3481 ttttccgtaa ctgatctttt cttcttgtaa gattgtaaga atcggcaaca atactgtatt
     3541 tagttcagta gttgttactt ggagctgacc ttttttataa gcgattccat cagaagctat
     3601 tgttcgctga atcgctgaca acacattttc tgggagtaat gggatgtcga tcgtcaaacg
     3661 atccgccgtc cgtaccatat cttttagaga ttctttggtt cctttagcaa tcacctgtcc
     3721 ttgatccatg atcacgatct ggtcgcaaag aatttctact tcttccatgt aatgtgttgt
     3781 atagacaatg gttgctcctt gtcgattcag ctccttgata ctttctaaaa ttttattccg
     3841 actttggggg tctacagcta ctgtcggctc atctaaaaaa attaaccgcg gcttgtgggc
     3901 aatcccacat gcgatgttca accgacgttt caagccacca ctaagttctc ctggacgaaa
     3961 cgaaacatat ttttctaatc ccactaattg aatcacctct tcaatcaagg ctttcctttt
     4021 ctctttttcg gaaacatata aaccgcaaaa ataatcgata ttttcttgca cacttagttc
     4081 gtcaaagacc gcaacgtctt gaggaacgac cccaatcatt gccttgacag cataattttc
     4141 aggagtcatt gttgtcccga acaatttaat ggttcctttg tcataattta acaaagaaag
     4201 catacagttg atggcagtcg atttcccact gccgtttgga cctaacagac ctaagacttc
     4261 tccttcttgt acttgcagat caaaatgatt caatgctaca aaatctccat agcgtttgac
     4321 taatttatca atttcgataa tcatcatgaa aaactccttc caaatttctt gttgatctga
     4381 ctataataaa gtgcaagaaa agaaactagt gacgattggc taaaccacac atgacatttg
     4441 tcacaatgaa ggagtctgct atgcataaat ctcggctact caacgaaact agctttctac
     4501 tggttatctc attgcttttg ctggtaagtc aaggattcgt tgctatgcaa gttatctatt
     4561 tattgatcgc cgttttattc actggtgtca gttatttact gcctacaaag ccaaattgga
     4621 ttttccaagt tattctattt cttttatgtg cctttttacc tgatcttgtc ctgtttctgc
     4681 cagcgacgat gcgagtgatc gcccctgaag aaagccacct tgattacttg tttttgttag
     4741 gttttttgtt gatttcgacg gttggtaatc atccccccat caaggtcgcc tttgtcgtaa
     4801 ttttgctgtt tttttccttt tacttacgaa aaaaagatca acgaacgacc gatcttgaga
     4861 aaaagtacat tgtctccaaa gatactcatt gggaaaaaca acaagagtta tcggaacaaa
     4921 ataaagcatt ggccctgtca caagaaacac aattagcctt gcaaattgtg gaagaacgga
     4981 accgaattgc ccgagacatc catgacaatg tcggtcatct gctgtcaagt gcgattttac
     5041 aaattggtgc gcttgaaatg atcaatcaag aaccaaaact aaaagaacca ttagaaaatt
     5101 taagtgccac cgttcacact gggatggatc ggattcgtca aagtgtccat gacctccatg
     5161 agacatcgat ctcttttcaa cactccttag agtttctgat cgctgatttt ccttcttctg
     5221 ttacgattga aggaaacttg tttgaaggat tgaatgaaac gcagcaaaac tgcttcttaa
     5281 tggtcatcaa agaagcctta accaacagta tcaaacatag tcatgccaaa accgttgttt
     5341 tacattttcg aacgttgccg gccttttatc gcttgcaaat cacgaatgat ggcaccagcc
     5401 cacaaaaaaa ttctgctccc ggtattggtt tgaaaacgat gcgtcaacgt gttcaagaac
     5461 aaaagggaca agtccatatt ttccaagaaa accagcaatt tcagttaacg atcattttac
     5521 caagggaggg acaccaatga taacagtagt cattattgat gatgatccat ttgtgacgaa
     5581 atcgttacaa accattttag aaagcacgaa agagatccgt gtgttaggca ttggtcattg
     5641 tgccaaggat gcgctagtct tatacgaaac ccatcgtccc gatgttttgc tgacggacat
     5701 ccgcatgcct aaacaaacgg gcatcgatgc tgccaaagaa attttggcta aatttcccaa
     5761 agccatcctt ttgcttttga cgacattcaa agatgaagaa tacatccgcg aagcattttc
     5821 catcggtgtc aaagggtatt taatcaaaca gaacttgcaa gcgattattc cttctgtcaa
     5881 atccgcttat aatggacagg ccgtatttgg taatgaaatc atcgagactt tcactcaaat
     5941 gatgaagaat aaaccgacga ttgatagtca tgcttatgct tcttttagtg aacgtgagct
     6001 tgcaatcatc aaagaagtag ctgccgggaa aaacaataaa gaaattgcag atgccttgta
     6061 cttgagtgac ggcactgttc gcaattacat cagccaatta ctagaaaagc tagatttacg
     6121 ggatcgcacg caattggcta tctattttta tcagcatttg caataagtct tatctaacac
     6181 aaaaaaacct gaatgatgct ccttcgcatc attcaggttt ttttcacatc ttctattcaa
     6241 ttgtcccgca aaccatttcc tcacgattga aagaatcaag gattcgcagg gctgccagga
     6301 ttcgttggat tgttcggcgt gttaggcgga aaaataggcg ccttgtctaa cgttttttct
     6361 agttttgatt cttcacgatc gtctcgttct acatcaggac tttcattcaa tttgaattct
     6421 ttttccatct catctaactt tttaaagtca ttgccagatt cttttgctaa tttcgctaat
     6481 ttatcttcgg tttctttagc cataaagtaa cgcccccctt ttgatttcag cttagcatat
     6541 cacactgaaa aaacaaaaga aaacgctaac caacgatcat ttttagctat ttctcatctt
     6601 taaaatcaag cattttttgc cggtataaaa tcgtagggag aactgttttc cataccaatc
     6661 atcggatcta ctaactggtt atcgatcact tctgctgtaa gaacgaaatc tttagaaaca
     6721 gcaggttcta cctcttcttt cttttcttct ccaacaaatg aagaaaacag atctggttcc
     6781 actgtttcca ttacttggtt tttcgtttca ttcgtcccac tgtcatcttc tacaggtagc
     6841 ttttctttta caacccgtgg ctcttttgta tagttcccat cttcaaatgg atcatttccg
     6901 accaaagaat cttcataagc tgctagtttg gtccggagag tgatattcat tttatcatta
     6961 tccatgatcc ttgatttcaa cgtagtcatc cggcttgctg attcattcgc tacacaagtg
     7021 acataggctt gctcttcccc taacaaaatc aacagttcct tactgcgagt cactgccgtg
     7081 tagagcaaat tacgctgaag catccgctga tattgatgga ccatgggcaa aataaccatc
     7141 ttgaattcac tgccttgtga tttatggatt gagcaacaat aagaaagagt gattttattc
     7201 cattcgtttc ttttataagt gacctcattt gcatcaaact ggataactaa ttcatccact
     7261 ttatcttcag aatcttttgc caagatgata cccgtgattt ctcccatatc tccattgaat
     7321 acattcaatt cgggagtatt gactaattgg aggactttgt ctcctatccg ataaacggtg
     7381 tcgttccaat gtacttcctt ttttccaggt ttccctggat tgaatatctc ctgcatcatt
     7441 ttatttaacg catcgatccc tgcaggtccc cgatacattg gtgctaatac ttggatatct
     7501 tgtgcacgga atcccttatt tttcgctttt tccacaattt tagagatcaa gggttcaatt
     7561 tgataagcat cacttcggaa ataggaacga tctttttgat tctttgtgaa atcttttggc
     7621 agacgtcctt ctttgatctc atgggcaaga ggaataatac tagaaccatc accttgccga
     7681 taaatctgat tgagttccat tttgggaatt tgttcgattt ccaaaagatc atggagtact
     7741 tgaccaggac cgacagaggg aagctgatct ttgtctccta ctaagataac ttgcatattc
     7801 gtcggaatcg ctttgaataa tgtgttagcc agccatgtat cgaccataga catctcatca
     7861 acaatcaaaa gtccgccttc taattctttc gtactgactg aaggtgtttt ttcccgtcca
     7921 ttcagcccca gaagacgatg gatcgtactg gcaggcagac ctgtcgtttc gttcattcgt
     7981 ttagctgctc ggccagttgg tgctgcaagt agaataggaa aaatttcttg ggtatatttt
     8041 gaaggatcca attccagtcc gttcaactct gcaaaaagat tgacgattcc gttgatcaca
     8101 gtagttttcc ccgtacctgg tcctcctgtt aaaataaata gaggggaacg gattgcttca
     8161 gcgatagctt gttcttgcga aggtccatac tgtatgccga atattttttc gatacgccga
     8221 atattcttct gcacttcttt ttcaggataa tggatttctt ttttacgact gagaaggcgc
     8281 tggatcgaat taccgatccc ccattcagaa aagtacaaac tgttttcgta aagattagtc
     8341 tcttcttgtt gtatctttcc ttcttcaaca agttggataa ttacagtagc aacctgatca
     8401 ggctggattt caattgggcg acttgtttct aaaatacgga tcgtttgctg aagcaatgtc
     8461 tccgccggta cgtatgtatc tcctgtttga actgcatgct gaaaaatctg atgcaagata
     8521 gctgctcgaa ttcttctcgg tgaatctgct gcaataccga tttgttcggc tagattatct
     8581 gcacgtttga acccgattcc ttctatgtct tcgactagct ggtacggatt ttcttcgatg
     8641 atatccaaag actcgttctt ataggcctgg tagatactga aagctaactg actaccgaac
     8701 ccgtaacgat tcagtcccat gatcacttga tccatcccgt ggtttagccg aatcgtctca
     8761 gcaagcattt ctctttttgg cttcgtcaaa ccggagattt gttttaatac cgaaggatca
     8821 gcaatgattt tttcgatagc atcttctccc agcaattcta caattttttc tgctgttttc
     8881 ttcccgattc ctgggaattt ttcgctggaa agatacgcta cgatcccatt tgcagttgtt
     8941 ggcttttctt gctcatatct ttcgagttta agctgccttc cgtatttagg atgatccact
     9001 aattctccat aaaaacgata agtctcattt tcttgaatat cgccgaaact tccggtgact
     9061 acaatctctt tttcacggta atcagctgtg gtttcactga tgtgtaccaa aagcactttg
     9121 taaaaattac taggattttg aaaaaaaatc gcggcaactg taccaacgaa ataaggcatt
     9181 tcttctggct gcatcggaag ccgcctcctt cttctatagt aaaggttctg gtgctgtttc
     9241 agttcctaaa aaatctgttt ggttccatac tttcagttca tatggcaata atctgcaagg
     9301 ctcttcagtc tctaaaatcg tcaaactact attgaacaat ccacccattt cacgcaactc
     9361 acttagctct tttcctgtca tccattgaat ggctgcagtt aaagaagctc catgtcctac
     9421 gaataaaagt gggccatctt tatgtcgggc aaccgcatct gctacaacag tttcgatacg
     9481 tgtgattgcc gattcaatcg tttctccgtg aaaaggagaa ggatcataca gatctaaatg
     9541 attacgcaaa ttcggcagat tatccggata tttctggtac atttcatcga tcgactgacc
     9601 ttctaaagta cccagcccta gttctcttag cttatctgta taaaaaatct ctaccggacg
     9661 agcaagctct ttgttgatac cttctgctgt atcacgtgct cgctttgctg tactggaata
     9721 aattgcttta aaaggaacat attttacctt tttcccaaat gctttgattt cttcgtaact
     9781 ttgtggcaaa agcggagaat cgccattgct cccttgaaaa cgtctttcct gattccattg
     9841 tgtcttccca tgtcgggtaa aatatagttc catacgcagc aaactcctgt ctttctttta
     9901 ttttactcta taataaactg tctaatccag cttgtcattt tcatagtgat gcgtcaattc
     9961 taaattaggg aatccttgct ggcgaagtgc ttcatacact atcattgcag ctgtattcga
    10021 taaattcaat gagcggacat gctcatcatt catagggatg cgtaaacact tttcttcgtt
    10081 ttcacgcata aatgtttctg gcaaaccggt tgtttctttt ccaaataaaa agaagtgctc
    10141 ttctccatca gcaaaatttt cgtcactata tactttatgg gcaaattttg agatcaaatg
    10201 tagtttacga ttacctaaaa atgttaaaaa agcttctaaa ttactgtggt aagtaatatt
    10261 tacatcattc caataatcca accctgcccg ttttagatgt ttgtcatcag ttgaaaaacc
    10321 tagtggctcg attaaatgaa gcggagtatt cgtggctgca cacgtacggg caatatttcc
    10381 agtgtttgca gggattaatg gttcaaataa aacgatatga ttcatcagga tcattcctct
    10441 catatttatg ttcgtttcta ttttctgaca atttgatttt tgcataaaaa aaaacgtatc
    10501 aactcggtgt cagtcactgc gagtcgttac gttagtgaag ctcttctcac taacttttat
    10561 cagaacagga acctgataaa aaccaatgaa aaaacaactt gttgctaata taacaccctc
    10621 aacttttttt tgcaacctct actcattaaa aactgctaga aaaacgtttt ctcttagttc
    10681 cttttaataa ttctatgtca attgtaatct tcttcaaagg attcttatat aagtctgctt
    10741 tgattcgctc ttctacctgc cattctaaag gtgacatggc caaaagatct ttttgatttt
    10801 cctctggtat ctcaaattca taggttacac gctgacgttc agttaatttc attgcttcag
    10861 caaacttggc tactacacgt tcattggaat aactctgttt ttcaggttga ttcggataaa
    10921 aagcctgacg tagttccttt aaataatttt cttgtggcac caccttcaac acttctccat
    10981 cttctgagag tacacgttgg aattcttggt aatgagaagg cgaaaagata tttaaaaccg
    11041 cgttgaatgt ttgatcagca aacggcaaat tggtcaaatc agctacacac caaaatgctt
    11101 cggcagattg ttctgtagct gcgtagactc cttcttttga aatatcaaat ccaacagcat
    11161 tttgaatctt tttcaatttt gaaagttcat tcaagaagct accttctccg cagccaacat
    11221 ctaatagcga gacttcttca ggtaaatgct gcgcaatagt ctcaataacg ggctgataca
    11281 tgccagattg gatcattcta cgccttggaa cgaacattcc ttgatcgtat tctgttttaa
    11341 ccgcatggga taaaaaataa agtgttcctt ttttggaaag gtcaaaacgg tgattatttt
    11401 tgcatatcaa gccattatca gactttttta cttcttcttg gcaaaccgga cagcggaaca
    11461 gctgatgatt ttctgccaaa aactggcgtc ctcggtcaat tttttttaac atagttcccc
    11521 ctcatttctg ttttatctta ccgttagatg ctcactttgg caactgctaa aaatatgata
    11581 caatgcctag aagaaagtga aagataaagg agaagattag atgatcaaag aattttgcgc
    11641 tgaaaactac acatctattc ctctagcaat tgctagagga gccgatcgga tcgaattgtg
    11701 tgacaactta gcggtcggtg gcacaacccc gagtactggt gtgatcgaag aaacgttaag
    11761 ttatgctggg gaaaaagata tccccgtaat gacgattatt cgtcctagag ggggcgactt
    11821 cgtctataac gatatcgaat tgaaaatcat gcatactgat ttgattgaag caaagaaatt
    11881 agggacggat ggcgtagtat ttggttgtct tactccttca ggctggctag acgaagaggc
    11941 cttagaacta ttgatcgaaa cagcagaagg gttgcagatc accttccata tggcttttga
    12001 cagtattcct gaagatcggc aattcgaagc aatcgattgg ctggtagaac atggtgttga
    12061 ccgtatcttg actcatggcg gtcctgctga caaaactatt gtagaaaatt tcccacggct
    12121 taaagaattg attgcctata cagatggccg cttaatcatt ttgcctggtg gtggtgtcac
    12181 tgcagagaat gcagcacatg tagctaaaga attgaacgta agcgaagtac acggaaccaa
    12241 aatcgtggat cttcagttat aatcttataa acaaaaaaac atttgcaaca ggcataatgc
    12301 ttattgcaaa tgtttttgtt tttttacata aaatagccca taaataaaga agcaatattg
    12361 aaataaatca gtacactcgt caaatcgctc aaagtcgtga taaagggacc gcttgctact
    12421 gctggatcaa atcctagacg atccattagc atcgggatta aactacctgc aagattcgcc
    12481 actgtgattg caaatagcat agccatccct atgacaaacc ccaagataaa attatgtttc
    12541 caaaaaccta cgattagaaa aatcgtgatc cccgtaacag ctcctgtgac aacgccggtc
    12601 aagacttcag ccaaaacaag tcgaacaaat ccatgatctt tctcatcaga aatcgcaagt
    12661 cgacgaacag ccacagctag cgactgggtt ccagcattac cggcagttcc tgtaatcagc
    12721 gaaatgaaga ctgctaaaat actcgcttca cttaccaagt cttcatagtg actgattaaa
    12781 gaagcggtcg acatacctaa aaacaacaga gtgatcagcc aaggcaaacg tcgagacgct
    12841 gcctttagtg gattttcatt aatttcttcc acattgaccc cggcaagtcc tgaataatcg
    12901 ctagcagctt catcatcaat aacgtcgatg atatcatcga ctgtgacgat tcctaacaaa
    12961 tgatcatcat aatcggtaac aggaagagct aagaagtcat aatctctaaa tgtttgtgcc
    13021 acatcttctt ggtcgtcgcc tacatgaact gaaagcactc gttcgctcat cagatcagaa
    13081 atcattgcgt cgtcatcatt cacgattagg tcgcgcagag aaataacccc tactagctga
    13141 ccttcttgat taacgacata gatgtaatag attgtttcag ctacatctgc ttcgttcttc
    13201 agcacgtaca tcgcagagcg aacagtttga ttggctacga tcgaaacaaa ttcagttgtc
    13261 atgatcgcgc cagccgtttc atcctcgtaa tggagaagtt ccttgatttc actggcatcg
    13321 tctgccgaca tcaaacttaa atatttagca atttgctttt tatccagcgt attaagcaag
    13381 tcaacagcat tatctgtata catctcagaa agcatatcag ctgcatagct aggacgcatt
    13441 tcatctaggt attctttcat atgttcgttg tcttcttcaa tcacatcgaa catatccgct
    13501 aattctttag gcgacagata ggaatagatc agctgcctgt tctcttctgt aagagattgg
    13561 tagaattgcc cctgctcata gatatgcatc tctaaaaaac tgtttctgaa tgcctgaata
    13621 ttattttccc ttaaatcttg agtcaactgg gcaaatcttt cctctaattc ttgattttca
    13681 tccaaaccaa tctcacctcg ccttattctt caaaccgtct cacaactgcc tccatgtctg
    13741 cggctaaagg ctgcttgaac tccagcaact cttctgtaaa tggatggtaa aaacgcagat
    13801 cataacaatg aagggcttgt ctttttattc ccagatccat tcgcccgcca tacatatcat
    13861 caccgagcag ggcacagcca attgcggaaa aatgaactct tatttgatgt gtccttcctg
    13921 tatgcagctg gatatcaact aacgcgattt ctgttagtcg cttctctatc cagtactcag
    13981 tctgtgccga acgtccttca tcagagacca ttcgcttcaa tagcgaagaa taatctcggg
    14041 cgatcggcaa atcgatatgt ccatgtgccg ataaatctga aagccttcca ctgactaagg
    14101 cctgatattt tttcacaaac ttcttttcac gaagttgcat atctaatttt gcgtgcgcaa
    14161 atccatgctt tgcaaaaagc atcaagccag atgtatcccg atctagtcgc gtcaccacat
    14221 ggattacttg gttttcgtaa ccttgttttt tataataagc ctttacacga ttagccattg
    14281 tcccatttgg atggtattgg gcaggaatag acgaaacgcc tgctggttta tttactacca
    14341 gaacgtgttc atcttcatac acgatatcga tcggtgtttc atccaaaagt acggtttcat
    14401 gttctccttc agcaggaatc acaatggtta ccttatcatc cttagctaga gaaaacaaga
    14461 cattttctac ttgatcatta actttgattt gaccgccttg aaattttatt ttcgctaaca
    14521 gaccctttga gatccctttt tcttttaaaa aatattttac ttgctgagaa tgatctttct
    14581 cataatccca tgtaaactgc atatcaatcc tcactaataa aggcatcttt cacacgatgc
    14641 caaaaatgca tatgtcgata agaagcaaaa tggatccgtt cttctgctat tcgataataa
    14701 attgagcgga tatttgcttt tgcgacattc aattgatcaa ccgttacaag ataatcttct
    14761 gtattttcca attttatttc tagccattca tcatgggcaa tcacgatcgg agaacccaaa
    14821 gtacgaaaca ctcgattatt cagtgaagca atctctgcca actgaaaggc attgatacta
    14881 ggatggatca cagcaccgcc gacactttta ttatatgctg tcgaaccagt aggcgtagaa
    14941 atcgtcaatc catctcctct aaaactttca aacagctcgt ttttgatgta tacatcggct
    15001 accatcgtac gattggcccg tttaatcgtt gattcattca gtgctaaaaa atgctggtcc
    15061 ggtgtttcat ccaaataact gatccgaaca tccagcaagg gataactaac actttgttcc
    15121 cggttcgttc gtaagctgtc tactagttct tccagctcgt aatcccgcca atccgtgtaa
    15181 aaccctaaat gacctgtatg cacgcccaag aaacgcacat cattcagaca atgactgtag
    15241 tgatggaacg ccgacaataa tgtgccgtct ccgccgactg agatgaccaa ttctggctca
    15301 tgatcatctc gttgaatccc tgcttgttct aaaagtgcgt tcaaacgttc tgtcacttct
    15361 atagtctttg cttctttatt atgaacgatc gctactttca tcccctgcct cctctctttc
    15421 gctttctttc ttcaaaagcg ttcgatagta gacatcatct gattttcctc ttccatggga
    15481 gaataaccgc tgtgcttctt ggatttcttc acggatactg gacatctctt catccaactg
    15541 ataggcagct tctgccgcac gctgaagtcg ttcactcatt tcttctggaa aaacgccttg
    15601 atatttataa ttcagcgaat gctcaatcgt tgcccagaaa ttcatcgcaa gagtccgaat
    15661 ctggatttcc gccaaaattt tcttctcacc gctgatcagc tgtacaggat actcgatcac
    15721 cagatgataa gaacggtagc cacttgcttt tttatttgtg atgtaatctc gttcttggag
    15781 aactttcatg tcttttcttt tacggatgat ctcaacaact tgatggatat cttctacaaa
    15841 ctgacacatg atccttaacc cggcaatatc agacatttct tcttctaaac gtcccattgg
    15901 aatatgccgt aattgtgatt ttgttacaat actatcgact ggcttgactc tcccagtgac
    15961 aaattcaatc gggacatgtc gattttgttc acggaactgt ttgcggatcc cccgcagttt
    16021 cactttcaat tctgatacag tttgttcata tggtgctaaa aatgcttccc aatctcgttc
    16081 catagctgat acctctactt cattaacatt cattgtttat tctaccatat atgcaaagtt
    16141 ttttcgtact cttgatttat gcattgacca aaaaaagaaa attaggcaaa atagacaaaa
    16201 aggagtcgat caaatgacac aaatgcttga aatcgaatat aaatctatgc tgacaaagca
    16261 agagtacgag atattcatcg ctcactatca attaacagat aaagatttcc gtgtacagac
    16321 taatatttat ttcgataccg ttgatgaaca gctgaagaaa caaaattgtg gcctacggat
    16381 ccgtttcgtt ggtcatcaag ctgaatatac attaaaaaca cctgctgaaa aagggcgtct
    16441 cgaaacaacg gataccttta taacagaaga agcagaagca tttatcttcg aaaaacgtct
    16501 gcctcgttca ggcgcagttt ttcaaaaact ttctgatttg aatatcgatc ctgcttcatt
    16561 aatccaaacc ggaaagctta caacaaaacg agctgaattc cctattgaag aaggactttt
    16621 agctattgat gaaagctggg gagacaatct acacgatttt gaactggaac tagaggtagg
    16681 ggatgcatct gtgggaaaaa ttgcttttat aaatttttta aaacgttttt ctttacctta
    16741 tcgtcctgct aagaacaaga tccaacgtat gcttgaagct aaaaattaga aacctttcat
    16801 tttcacaagg cacttgagtt cattttttta cttttttctg ttaagttata ggatgtgaaa
    16861 gaatctagtc actagttgta tagaatctat tttgggggag attcatatga tcgaaatcta
    16921 tttatttgtt aatcctttag gggaatactg tttcgaaatg gaacaacagt tattacaatt
    16981 tatcgaagaa gagtacggga caccatcaaa agaaaagatg caatttcgct ttcttccttt
    17041 ggtcaatttg cagacaattg gggatgtcat gcaacaaaaa ggaatttctc agaacaatct
    17101 tgaggaacgt aatcacttat tctcaatgac ttattctgca gcattggact gcaaagcggc
    17161 gcagtttcaa ggaaagaaaa aaggccgccg ttttttgatc aaattgcaag aagcagtcgg
    17221 ctgtaatgac ataccatatt ctcaagaact tgctgaatcc atatttgaag aaatcggcgg
    17281 cgatttgaat atgtttaaag aagatcgtca gtctgacttc gttaaagaag tattcttttc
    17341 tgatcaaaaa attgctcggg aaatgggcat cacaaaacac ccttctgcag tcgtttacaa
    17401 ttatgcctgc gaccgtgatt ttggtgtttt agtagaagta aaaggtacaa gtagcctttg
    17461 gagaatcaca gagctgtgta aaacaacaga taactcatat caagttttta atggtgaaca
    17521 tttacataca aatgattctc agttccattc aagaactagc catttacact tgctttctaa
    17581 ttaaaaa
//
LOCUS       DACDRN010000047        17232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDRN010000047 DACDRN010000000
VERSION     DACDRN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.047"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..369
                     /locus_tag="IWJ80_001912"
     CDS             1..369
                     /locus_tag="IWJ80_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9461624.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="IWJ80_001913"
     CDS             549..1490
                     /locus_tag="IWJ80_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP9461625.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="IWJ80_001914"
     CDS             1559..2290
                     /locus_tag="IWJ80_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP9461626.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="IWJ80_001915"
     CDS             2434..4614
                     /locus_tag="IWJ80_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP9461627.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="IWJ80_001916"
     CDS             4634..5116
                     /locus_tag="IWJ80_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP9461628.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="IWJ80_001917"
     CDS             5132..5893
                     /locus_tag="IWJ80_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9461629.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /locus_tag="IWJ80_001918"
     CDS             6024..7358
                     /locus_tag="IWJ80_001918"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP9461630.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="IWJ80_001919"
     CDS             7665..8150
                     /locus_tag="IWJ80_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP9461631.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="IWJ80_001920"
     CDS             8143..8826
                     /locus_tag="IWJ80_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP9461632.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="IWJ80_001921"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="IWJ80_001921"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP9461633.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="IWJ80_001922"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="IWJ80_001922"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP9461634.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /locus_tag="IWJ80_001923"
     CDS             12442..13071
                     /locus_tag="IWJ80_001923"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP9461635.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="IWJ80_001924"
     CDS             13123..13455
                     /locus_tag="IWJ80_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9461636.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="IWJ80_001925"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="IWJ80_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP9461637.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="IWJ80_001926"
     CDS             14281..15654
                     /locus_tag="IWJ80_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP9461638.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="IWJ80_001927"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="IWJ80_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP9461639.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..17149
                     /locus_tag="IWJ80_001928"
     CDS             16709..17149
                     /locus_tag="IWJ80_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9461640.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5876 a         3009 c         3795 g         4552 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aatattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 atacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17161 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17221 tgggaatttt tt
//
LOCUS       DACDRN010000048        17133 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDRN010000048 DACDRN010000000
VERSION     DACDRN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.048"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..127)
                     /locus_tag="IWJ80_001929"
     CDS             complement(<1..127)
                     /locus_tag="IWJ80_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP9461641.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(231..542)
                     /locus_tag="IWJ80_001930"
     CDS             complement(231..542)
                     /locus_tag="IWJ80_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9461642.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(544..741)
                     /locus_tag="IWJ80_001931"
     CDS             complement(544..741)
                     /locus_tag="IWJ80_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP9461643.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1135..2862)
                     /locus_tag="IWJ80_001932"
     CDS             complement(1135..2862)
                     /locus_tag="IWJ80_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9461644.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2855..4048)
                     /locus_tag="IWJ80_001933"
     CDS             complement(2855..4048)
                     /locus_tag="IWJ80_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9461645.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4235..4879)
                     /locus_tag="IWJ80_001934"
     CDS             complement(4235..4879)
                     /locus_tag="IWJ80_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9461646.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4973..5107)
                     /locus_tag="IWJ80_001935"
     CDS             complement(4973..5107)
                     /locus_tag="IWJ80_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP9461647.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="IWJ80_001936"
     CDS             complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="IWJ80_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP9461648.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7238..7711)
                     /locus_tag="IWJ80_001937"
     CDS             complement(7238..7711)
                     /locus_tag="IWJ80_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP9461649.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8112..8336
                     /gene="nrdH"
                     /locus_tag="IWJ80_001938"
     CDS             8112..8336
                     /gene="nrdH"
                     /locus_tag="IWJ80_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP9461650.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8495..10654
                     /gene="nrdE"
                     /locus_tag="IWJ80_001939"
     CDS             8495..10654
                     /gene="nrdE"
                     /locus_tag="IWJ80_001939"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP9461651.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10704..11669
                     /gene="nrdF"
                     /locus_tag="IWJ80_001940"
     CDS             10704..11669
                     /gene="nrdF"
                     /locus_tag="IWJ80_001940"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP9461652.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11758..12897)
                     /locus_tag="IWJ80_001941"
     CDS             complement(11758..12897)
                     /locus_tag="IWJ80_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9461653.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13206..13919)
                     /locus_tag="IWJ80_001942"
     CDS             complement(13206..13919)
                     /locus_tag="IWJ80_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP9461654.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="IWJ80_001943"
     CDS             complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="IWJ80_001943"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP9461655.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15108..16640
                     /locus_tag="IWJ80_001944"
     CDS             15108..16640
                     /locus_tag="IWJ80_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9461656.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16831..17010)
                     /locus_tag="IWJ80_001945"
                     /pseudo
     CDS             complement(16831..17010)
                     /locus_tag="IWJ80_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(17030..>17133)
                     /locus_tag="IWJ80_001946"
     CDS             complement(17030..>17133)
                     /locus_tag="IWJ80_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9461657.1"
                     /translation="LESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         5317 a         3523 c         2929 g         5364 t
ORIGIN      
        1 atgaaaaaac acattccact aaatttgatg ccggttttcc ttgaggtttt gttccagcac
       61 ctcctttaat ttttttaagc tcttgatctg aacattgttt agcatttagt ctattttttt
      121 cttccattac gattccccct acaaactata aatactttta tgtcactgat tcaatatacc
      181 tatttttcta atcaaaaaat tttgagacat atttaatatt ataagtatca ttaaaattga
      241 gatttatctc cataatctgc tcgatataaa ccatttattc cagcagttct tccaatttca
      301 ctcaaatttc ttattaattc ctcttgatat tcagtgaatc ttattttatt agtataagca
      361 gtaaaatata gataattcac caatctattt attaaaggac tgggatctaa ttcatatttt
      421 tgtttttcta attttaaata taccttttgt aaaacctcaa gaatatcaga atatttagga
      481 tctttactta tagatatatc attatataat tcatgaacaa tttgcttagc attttttttc
      541 attttagcac ttccctggaa ttgctccacc taaaaaacca cctatagaca ttcctgcaat
      601 acaagtagtt gccttggccc aatcgaccgt acatttattt ttagtgcaat acactccatt
      661 tccataatat tttccactat gagtggtacc cccatagata agttgtgtct ctttaataga
      721 caaaattttt aaatgtttca taatctaatc acccctaaat taatgttttt agactattat
      781 tctaacattc attaaatttc aaaaaacaat tccttcttga aacgtagcaa aataattcct
      841 gaataagaat ttaatcactt ttaagtatat aaactatgta tattctttaa tattttttga
      901 gaatgcactt cgtacataat aaactttaat cacattagtc tttcatacct acgatcgatc
      961 tctttgactc gcacccataa attcataata gtttgtcggc gtctttgaac gtttcgaaga
     1021 tgagctacgc attctctttt tttatagaca aaaaagatag agacgaacat ggagcttcca
     1081 aatttggata tttgatccaa ttttggaagc tccatttatt tcgtgtctgt ctttttattt
     1141 tttccagctt tcgactaatt cttggttctc tttgatccag tcttttgctg cttgctgtgg
     1201 atcttttcca ttgttgattt ccaacataac tttttccata tctttttctg accaatggaa
     1261 attgtctaat actttgtaag cttctggctg gtcttctttc aatccttttc gaaccatcgt
     1321 atggatctgc tcttcgcttc ccattccgtt ttcaggatct tctaagtatt tcagatcata
     1381 tttggcaaac atccaatgag gtgtccatcc tgtaactaca attggttcgt gttttttgat
     1441 cgcttgacct agtgcaacgg tcattgctcc tgaagaagat gtctctactt tccagtcttt
     1501 caagttgtca tatttttgga tcgtgttttc agcagccgta actacaccag ctcctggctc
     1561 gattcctatg attttcttac ctgcctgatc ggttaactca tcgatcgaat ttacatccat
     1621 gtaggctggc actgctaaac caacttttgc tcctgtcaaa ttggcaccta ggtcttcgac
     1681 ttgatcttta tattgtgcat attgtgaacc atgagtttta ggtagccaag ctgaaaccat
     1741 ggcatctgac tcgccatttg aaacagattt ccacatgatt gaattatcta atggcgtcat
     1801 cgttacatca tagcccattt gtttcaatac ttcaccgact acatttgttg aagcaacttc
     1861 tgtatcccat tcaacataag ataagttgat acgttttgtt tctttggcag atgaaaaact
     1921 ccctatacca atcgcaccta agatcactac tactacagca ccaatgatca gtccttgacg
     1981 ttttttactt ttctgtgttg ttgctcctgc agctttttta ttgtttggct ggttcaaatg
     2041 ctgtgtaaaa cggtcaatga tgattgctaa gataactaat gcaaccccgt tgacaaatcc
     2101 attcccgact tgtgctcttt gcaatgctga taatacgccg cgtcctaagc cgggtgcacc
     2161 gatcatagag gcaataacga ccattgaaag tgacagcatt gttgtttgat tgatccctgc
     2221 catgatcgta cttttagcta aaggcaattc tagtttgaat agtttttgac gacttgtgct
     2281 cccgaatgaa tcagaagctt ctactaattc tttaggaact tgtcgaatcc ctaaattcgt
     2341 aaaacgaacg gttggtggca gagcaaagat gactgaggca aatacccctg gaaccatccc
     2401 gataccgaaa aatgcaactg ctggaatcaa ataaacgaat cctggcattg tctgcataaa
     2461 gtcaaggatc ggtttgataa tactttgggc tttactgctt tttgccatca agatccctaa
     2521 tggtacccca atgatgatcg agatcacact agataaaaga acaagtgtga cagtattcat
     2581 caaatcattc catagacctt ggttataaat aaaccataga ccgattaatg tgaacaacgt
     2641 aagtcctgga cgtttcttag aaataaagaa agctgctacc gtcaataaca agataaacaa
     2701 aggagctgga atcactaaca gtaaatttgt aatccccgac atcactgcct gtccaatcgt
     2761 ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac cattctgtga tattttctac
     2821 ccagtttgcc actggtaatt gatagttcaa tagattattc atgttcactc acctcgcttg
     2881 tttctgctaa tgcttcaatc acactacctc ggatgacgac accttttaat tttccatcct
     2941 ctacaactgc aagtggagtc ggtgagtcat aaataatatt gaaaatatca cttaccacca
     3001 tgtctttccc aatcaccgtc acattttcat ctaggaagtc gatcaatgga cgtttttgct
     3061 tcctagcttc taacgcttga tctgcagtga tgatcccttt caggtgtctc tttctgtcaa
     3121 cagccatcag catgctgact tcttcattac gcatccttgt caatgcaacg tttggtccat
     3181 cagattcgat attcgtcgtc aatgcaggta ccatgatgtt ttgagccgta agcactttgg
     3241 aacgatcgac ttcttccaca aattcacgta cataatcatt tgctggattg gtcaatattt
     3301 cttcacctgt tccgatctgc atgatttcgc cgtctttcat caaggcgatt ctgtccccaa
     3361 tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat cgttttttga acattggctt
     3421 gcaggtccag taattcgtct tgcatttctc gccggatcag cggatcaaga gccgagaacg
     3481 cttcgtccat cagcaagatc tcaggatcat ttgctaaagc tctggctagg ccaacccgtt
     3541 gttgcatccc accagataac tgactaggat attggtcttt aaatgaaagc aaactagaat
     3601 tttctaatgc tttttctgct ttttcttgtc gttcttcttt aggtacacca cggacttcta
     3661 acccgtattc tgtattttcc aagatcgttc gatgtggaaa cagaccgaag ttttggaaaa
     3721 ccatattgat tttatgacga cggacttctc gcaattcttc tttgcttaac ttcgatacat
     3781 cttctccatc aatatagata ctgcccgaag ttggctcaat cagacgattc aatagacgga
     3841 tcaatgtgga tttcccactc ccggaaagcc ccatgatcac gaagatttct ccttctttta
     3901 cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt tttctttaaa atctccgttt
     3961 tcggctggtg atcattcatc atatctaaag cggcttgtgt ctttttacca aatatctttg
     4021 ttaaatgttt tacttctact tttaccaaac aaattctcct tttttatgta aattcatttt
     4081 tctttctgaa aaaagtgacc acactatgtt aacatcatgt agtcactttg tcaaaaatga
     4141 aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa tctttgtaaa taaaaggttt
     4201 agccaagtta atattttttt aatatttcac acaatcattc tgcaagctga tgctccgggt
     4261 aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc aataaaatga acgatgtccc
     4321 ctgccgtaaa tttagcatag gggccaggtg aaacaagcag ttcttcatta tgagaaattg
     4381 cgataactgt ggcgccagtc gcctgccaga tattcaactc gctaattgaa cggtccagat
     4441 gcattgcttt ttctgataaa gataattcaa acggaagtaa aggattcatc ttatgtattc
     4501 gcttcgtttg attgaccagc tgatccaata attgagaaaa atgattcagc tcatcttgtt
     4561 gcttctccac acttgccatg atttctgctt ttgtatcttg gatggattgt acatcttgat
     4621 attgcaatag gaaatctttt gctttttcct tcgaagcaac aaatgcccca cttccatgcc
     4681 gtacttccat gattccaaga tctaccagca cattgatagc cttgcgggta gtctcaggag
     4741 aagcattgta cgtactggca agtgttgagc gggcatggat cttctcaccc actttatatc
     4801 ggttttcaac gatacgttcg gctaaatcaa cagcaatctg ctggtagcgc gggcggacga
     4861 tttttttctt tccagacatt ttctttcctc tttctttttt tactttccct catcttacta
     4921 tgctctagca aaataagcaa aaaaggggag aatagcctct tgcaacgact gatcatcgct
     4981 tgatctgttc cttttttact gggcaagctg tttgacagtc atcacaattc ttaccggatt
     5041 ttaaacgagt atagacaatt gcgcctgcac ttccaaagat taacacactt aataagaatg
     5101 tcgccatcct attcctctcc ttctaatgac aatatagtat aaaactggtt cttggatttt
     5161 ggctgacgga acaaaacata aacgagtcct atcatcagta aaatagcaag atacgtccct
     5221 aacgctacct gtccaccctc caataagtgt cccaactgat aaatcacaaa actaactaaa
     5281 taagctaaac cgcattgata gcctatggcg atccaagtcc atttcttggc attcatttcc
     5341 cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa gtaaattgaa ggctaacaat
     5401 gaataagctg ctgcgggagt atatgtgtgt tgtagcactt gccagatttc tctgccattt
     5461 tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc caataatatt ttctttagcg
     5521 accaatcctg taatcgctgc gactgttcct tgccagtttc cccaaccaag cggctgaaaa
     5581 acaactgcca ggattcgccc gaaaaaggct aagatacttt ggtcttcaga aacaggttga
     5641 gcataaaaat tataagaaga gctgaaccag atcacgatac tagaaacaaa gataatcgtt
     5701 cctgcttttt taacaaaaga cttgctgcgg tcataggttt gtcttaggac cgctgaccat
     5761 tgaggaaaat gatatgcggg caattccatg ataaaaggag ctgtatctcc agcaaacaaa
     5821 cgtgtttttt tcaagctgat tccagagaaa ataattgcag tcataccaag aaaataggca
     5881 gaaggagcta cccaagattg atgaggaaaa aaggcccctg ctactaaggc aataatcggc
     5941 aactttgcag aacaaggcat gaaggttgtg accatgatcg tcaaacgacg atcttgctcg
     6001 ttttcaatcg tgcgactggc catgacgcct ggcactccac atcctgtagc aatcagcata
     6061 ggaatgaagg attttcctga aagaccgaat ttcctgaaaa gtcgatccat gacaaaagcg
     6121 atcctcgcca tatatccgca gtcttccaga aagctcaagc ataaaaacaa tataatcaat
     6181 tgcggtagaa atcctaacac tgctcccacg cctgcgatga tgccgttcaa gatcagctcc
     6241 tgcatccatc ccgctacttg ccattcagct aacaatctgc caacatgttc tggtaaccat
     6301 gtaccaaaca atgtgtcatt gatccaatct gtccccattg ttcctactgt ttgaatagcc
     6361 agataataga tcagccacat cacaaatgcg aagatcggca aggctaacca acgattcgtt
     6421 gcaatctgat cgattttgtc actcatcgat aattggaaag aatcatttga cacaacacag
     6481 agtgtacata gttgggtaat aaattcataa cgttcattga ccagaatcgt ctcactatcg
     6541 tcgtccaata gtttctctgt taactggatc agttgctcga tctctttttg ctgagaaact
     6601 gctatctcgt aatcctctct taccttctga tctcgttcaa aatatttcat tgcataccag
     6661 cgtttcttgc cgctttgttc tcctagtaca tcttcaattt catgtaaagc tgcttctaaa
     6721 cgtggatcat atgtacaggg aacaggaata gaaggccgct tttttgcaga ctcctgaatc
     6781 ttttcgatta gtttttcgat tccttgcttt ttcaatacgc tgattggtaa aactggtaca
     6841 ccaagaccat aggaaagctt ttctagattg attttttttc cttctttttt gatgacatcc
     6901 atcatattca gaccaatgat cataggaatg tctaaatcca ataactgcgt agttaaataa
     6961 agatttcgtt ctaaattggt agcatcaata atgttgatga tcagttcagg ctgttcctga
     7021 accaaataat ctcttgcaat cacttcttct ggtgtataag gagacaaaga atagatgccg
     7081 ggtaggtcct gaatatgaat gttcggattt tttttcaacg atccttcttt tttttctact
     7141 gttacgcccg gccaattccc tacatattga ttcgaaccag tcaagagatt gaacaagcta
     7201 gttttcccgc tgtttggatt tccgactaac gcgatattca ttctttttcc tccctttgta
     7261 tcaagattag ttcggcctct gactttcgta acgtcagttc ataccctcgc actgtcagtt
     7321 cgattggatc tcccagaggg gctagttttc ggataactat ttttacattt cgcgtcaatc
     7381 ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg gattgctacg acgatcccag
     7441 tctctcctgc ttttaattga tcaagagaag tccagctttc agaggaagca gtctttttat
     7501 tgacaaagat ttgacctagt atttcccgac ttactgccac tcgatgactt tttaccagta
     7561 aaatggcatt gttgcctgat aaattgacga cgacgatttt acttccggca acgatcccga
     7621 tattttgcaa atggcgtttt gttttttctt ctgtttcgat tcgttcgact tgataaacga
     7681 aattctgctt tgcctgcgct aaggtctgca tattgattcc tctcttgtga aataaatcat
     7741 gtattaaaat cattcattat atgttctaat tttcttacgt gttacgcaaa taaatgagaa
     7801 tcattctcac atagaatact ctcggtaatt gagaatgtca attatacttg tgatattatg
     7861 agcgaatctt tagtattgat agataaaaat gagaaaaaaa tatttcttca tagttttcca
     7921 caattttttc tctattaaat ttgctatttt cccacacgat acttgttttc agctcttttt
     7981 tcaaaaacac aaaacttagt attccacagt ttccacaaca caatatgtag tgtttgttgt
     8041 ttgacttttt tactaagagt tttatactat ctattataga taataagaat gtgttagagg
     8101 aggcactcac catgaacgta aaaatctttt ctaaaaataa ttgtatgcaa tgtaagatgg
     8161 tcaaacggtt tttaacagaa aatcaaattg cttttgaaga agtcaatatc gatgaacagc
     8221 ctgaagcagt agactggttg aaagcacaag gattccaaag tgttcctgtc attacttctg
     8281 atgcagaaac agttatcgga ttccgtcctg atcagttgcg taaacttgcc agctaacatg
     8341 actaaaagaa tcaaaacgtg atacatggat ttgaagggct tgagacagca ccagtttttg
     8401 tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat gcaatgattt gcaatcaagc
     8461 atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt cttaaaaatt tacaagatgt
     8521 cagttatttc aagctgaata atgaaatcaa tcggccagtc aatggtcaaa tccctttgaa
     8581 taaggataaa gaagctttag ttgccttttt tgaggaaaat gtcaaaccaa atacgatgac
     8641 ttttccatct attatggaaa aaatcaatta cttgatcgat caagaatata tagaagcaga
     8701 attcattcaa ttgtattcac catcatttat tgaaaagcta tatcaatttt tggacgagca
     8761 acatttttct ttcaaatctt ttatggctgc ttacaaattt tattctcaat atgcgttgaa
     8821 aaccaacgac ggttccgctt atctggaaac ttatgaagat cgagtagcct tcaatgcgct
     8881 ttactttgcg aacggggatg aagaattagc acttgctctt gcagacgaaa tgatccatca
     8941 acgatatcag ccagctacac cttctttctt gaatgctgga cgtaaacgtc ggggcgaatt
     9001 ggtgtcttgt ttcttgactc aagtcactga cgatatgaac tctatcgggc gctcaatcaa
     9061 ctctgctttg caattgtccc gtattggtgg cggcgtaggg attactttat caaatcttcg
     9121 tgaagccggc gctcctatca aaggatatga gggtgctgca agtggtgtcg ttccagtcat
     9181 gaaattattc gaagatagtt tcagctattc taatcaatta ggtcagcgtc aaggagctgg
     9241 cgtggtttat ttgaacgttt tccatccaga cattattgct ttcctttcag caaaaaaaga
     9301 aaatgcagat gaaaagattc gcgtaaaaac tctttctctt ggcgtgatcg tacctgataa
     9361 attttatgaa ttgacacgta aaaacgaaga tatgtattta tttagtccat atagtgtcga
     9421 aaaagaatat ggtgtcccat tctcttatgt aaatattaca gctgagtatg atcatctagt
     9481 tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt gatcttgaaa atgaaatctc
     9541 aaaattacaa caagaatctg gctatcctta cgtaatcaat attgatacag cgaaccgcga
     9601 gaatccaatc gatggcaaga tcatcatgag taatttatgt tcagagattt tacaagttca
     9661 aacgccttct gtgattaacg gcaaacagga atatgaagta ttaggaacag atatctcatg
     9721 taacttaggc tcgacaaata tcgtgaattt gatgcaaagc cctaactttg gtcaatctgt
     9781 gcgtacgatg actcgtgcat tgacatttat tacagatgca tctgatatcg atgtcgtacc
     9841 accgatccaa aacggaaata agctgaatca tacgattgga ttaggtgcca tgggcttaca
     9901 cacttatctt gcaaaagaac aaatggaata tggctcagag gattcattag actttacaaa
     9961 tatttacttc atgctgttga attattggac acttgttgaa agcaacaata tcgctcgtga
    10021 aagaaaacaa gttttccata attttgaaaa atcagcttat gctgacggca cttatttcga
    10081 aaaatacgta actggagaat tccagccaaa atcagaaaaa gtcaaagcat tgtttgaagg
    10141 catttttatc ccaactgctg aagattggaa tgctttgaaa caagcagtaa tgaaagatgg
    10201 actgtatcat caaaatcgct tggcagtagc accaaatggc tcaatttcat atatcaacga
    10261 tacgagtgca agtattcatc ctatcactcg catgatcgaa gaacgccaag agaagaaaat
    10321 cggtaaaatt tattacccag cgccttatct atccaatgag acgattccgt attacacttc
    10381 tgcttatgat atggacatgc gtaaagtaat cgatgtttat gcaacggcac aacaacacgt
    10441 cgatcaaggc atgagtttga ctttattcat gcgttctgaa atcccagaag gcatttatga
    10501 atggaagaca acaacgaagc aaacgacacg tgatttgaat attttaagac attacgcttt
    10561 ccacaaagga atcaaatcca tttattacgt acgtaccttt actgatgatg cagaagaaat
    10621 cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat agatagattc gcttattcat
    10681 attttaatta aaggagcatt ctcatgtcag aaacatatta tgaagcaata aactggaatg
    10741 aaattgaaga tatcattgat aaatccactt gggagaagct gacagagcaa ttttggctag
    10801 atacacgtat cccactttca aatgacctag atgattggcg tacattgtct caattggaaa
    10861 aagatacagt aggacatgta tttggcggtt tgacactgct agatactgtc caatctgaaa
    10921 gtggcatgga tcagttgcgt aaagatgtcc gcactcccca tgaagaagcc gttttgaaca
    10981 atattcaatt catggaatcc gttcatgcca aaagttattc ttctattttt agtacactga
    11041 atacgaaaaa agaaatcgaa gaaatttttg actggacaaa tacgaatcct tatctgcaaa
    11101 aaaaagctga gcgtatcaat gaaatctaca aacacggtac accgctagaa aagaaaattg
    11161 ccagcgtctt tttagaaaca ttcttgttct attcaggttt ttatactcct ctttattatt
    11221 taggaaacaa caaattggct aacgtagcag agatcatcaa actaatcatc cgagatgaat
    11281 ctgtacatgg aacctacatc ggttacaaat tccaattagg attcaacgag ttacctgaag
    11341 cagaacaaga aacgttaaaa gactggatgt acaatcttct ttatgagctt tacgaaaacg
    11401 aagaacgtta tacagaagaa ttatacgatc caatcggctg gacagaagaa gtcaagacat
    11461 tcctacgcta taatgccaac aaagcattga tgaacctagg gatggatcca ctctttccag
    11521 atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat ttcaacaggc acaagcaacc
    11581 atgacttctt ctcacaagtc ggaaacggct atttgttagg tcatgtagaa gcgatgaaag
    11641 acgacgatta tttgattgga ttggaataag gatttatttt aattcaaaaa tttttcgaaa
    11701 aataaaaaga gtttagcgta cagatatttt aaatgtacac tgaactcttt tttatgctta
    11761 ttcacttgtt tcttccagca cttttggact gtctaacttt tccgaaacat gttccattct
    11821 tctcaataaa tagattttcc ggatatttaa tattatcgtc ttaatcactg catagacagg
    11881 tatgcaaatc agcatgccta atattcctgc gacacttcct gctccaatca ataccaagat
    11941 gatcgtcaaa ggatggatat tcattgattt cccaaataac aacggcttga tcaaatttcc
    12001 atctaattgt tggatcacta aaatcgacaa cgccatatac aaagcttgaa atggagagat
    12061 aaacaaaccg acaatgattg ctggtaccgc tccaataaaa gggccgacat acgggataat
    12121 attcgtgatt ccgcaaatga ccgcaagcaa caagctataa ggctgatcga atattctcat
    12181 tgccaaaaag ctcattacac caataatcaa agcatctaag atcgtactgc tgatataggc
    12241 agataatgtt tcgtttaatt cttttaccgt ttggcgtaac tctttacgaa tcgctttcgg
    12301 aaagaagtga gacaacgcat caataaattt gtgcccatcc ttgaacataa atagcaaaat
    12361 aaatggaacg gtaaataaca aaataaaaaa ttgcatcata aatgaaatga tttttgagac
    12421 acttgcagat aaactgacta atatgatatt gaacaaatta ctaagagata aatttgctga
    12481 tgctaattgt tcttccaaat tgaaattttt aaactcttcc cgctgcgcca attcatttaa
    12541 ccatttgctt gtttcatctg catacattgg caagctctgg gtcaattgaa ttgtctgttc
    12601 cattaactga ggcactacat tcatgacagt taaaacaact aaaatgatta cgaccaaaaa
    12661 cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt tcgagaaaaa caacgattgg
    12721 atcgaacata taatagataa aaccagcaac aagcaatggt aaaaaaattg ccgaaagcat
    12781 tttcaatatt ggactaaaaa tattcggcat ttgtaaaata aagtacacgc cgatgatcgt
    12841 gagtactagc tccactgtcc agaaaaataa ttttgattgt ttaaaacgtg attgcataca
    12901 tctcctccaa ggtcctttga tacttctatt ctatcattta acaaagagtt acgcgctgag
    12961 attttcattt gcttaaaaaa aacaaaaaaa ataaacatat tttaatgagt agacgagtac
    13021 ttaaacaaaa acacggtttg gagtagtgac aaaaattata ctgcagtccc tttttccaaa
    13081 taaacggtgt tcaaaagctt gcatcttcgt aataagccca gccaaacgaa aaatataagg
    13141 aactattttt atttggctgg gcttattact tgatgtagga catctatttt acaacctcag
    13201 atcatttatt gcgcaatact gactaaatgc aactttgtga tatttttgtg tacggacaac
    13261 tcctcgatca catcggcata agtgagccct tttggaagat caatcgtata gatatttttg
    13321 tacactcggt agtcatcaac gaattttacg tcaaaattca catcttcgat tacgatattt
    13381 ttctcttcaa aataatcggt aagaaaatct ttggtttctt ttctatggat aaattggatt
    13441 tctaactttt tagtcgtagg tacatggatc acgcgtttca ctactgtgag tgcaatagca
    13501 atcccgataa aactgatgat cgcaatggaa tagtaaccca tgccgatggc gatccctaat
    13561 ccagcaattg cccataaaga agcggcagtc gtaagtcccg tcaccgattg tttcgtgacg
    13621 atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca cttgagcaat cagccttgct
    13681 tcatctgaac gaatcgttcc agcaagctgt ggatactcta aagcatctct taatgcacca
    13741 ctagcgatct caacttggat caatgcaatg atacatgctc ccatacaaac taaaatatgc
    13801 gtacgcatac ctgctggacg atttttatat tgtcgttcaa aaccgattgc tccgccaaat
    13861 agcatagcga tccctaaacg taaaataatc tctggaattg tcaataccat atcattcatc
    13921 taaacaccct ccagtccaat atatcatgtc tgaaaggagc agacaaaaaa agtgcaattt
    13981 ttacctctta cgaaaaaagg actgtgacaa aatttttgtc atagtccttt ttatgaataa
    14041 acggtgttca atcagctgac cctcggtatt actcaacaaa cggcaaaata tggggagcta
    14101 tttttccttt gtcttctcta atactcaggt caaaacgctg atttcacaac ctctttagcc
    14161 tactattgaa aattacaact tacttgctgc ttcttcatcg atgatcacaa caacattgtc
    14221 gtgattttgc aatacacttg ctggcaaatg attcgttaca ggtccttcaa tcatcccttt
    14281 gattgcatca gctttgcttt cgccaaatgc catcaagacg atttctttac ttttcataat
    14341 tgaaccgatt cccattgaaa cagcttttgt tggtacatct tctgctttat cgaagaatcg
    14401 tttgtttgca ttgattgttg attctgttaa gtcaactact gaagtcgttc cgtcaaatga
    14461 agcaccaggt tcattgaaac cgatatgtcc attttgcccg attcctaaga tttggatatc
    14521 gattggatgt gcatcgatga tttgatcata ctttttgcat gctgcttcca gatcttctgc
    14581 ttttccatct ggtacaaacg tttctttgaa tggtttttta tcaaataatt ggacatccat
    14641 gaaatgacgg tagctttgtt catctgaacc gcctagtcca acgtactcat ctaagtttac
    14701 agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt tctttatata aagtaactgg
    14761 tgtgcttcct gttgccaatc ctaatacttt tgcacctttg ttcatacctt ctttgatgat
    14821 ttcaaatgct ttttttccgc cttcttcagc gttttttaca cgaatgattt ccatagtgat
    14881 tgctccttta tattttggta tagcccaatt atagcattct ttttttcttt ttacaataat
    14941 tagtatagac caaattgcat ttttttcatt ttcttgacaa taaataggaa tccgtgttag
    15001 gtttcataag atggttcaat caaacaatcc atgttgcatg aaaaccaagc aagtgattca
    15061 ctgtggacgc tttatgtctg cagttttttt atgaaaaagg agttattatg agtatactga
    15121 cattaaacaa cattacccag caatttggcg acaaaatcct ctatgaaggt gtcgatctgc
    15181 aattaaatgc tggcgaacat cttgggctga ttggacaaaa cggagccggt aaaagtacat
    15241 tgattaaaat catcactggt gagatcttac cagatgacgg acagatccat tggcaaaaaa
    15301 atattcacaa aggttacctc gatcaatatg tggaagtcga tgaaacatta acaatcgaag
    15361 aatttctaaa aactgcgtac gcaaaagaat acgaaaagga agaaaaaatt gcttttcttt
    15421 accaaacata tagcgagacc ttcgatgatt ccttgctgga aaaagctggg aaactccaaa
    15481 cggaattaga ccaaggagtg ttttatcaaa tcgacacctt agttgctgag atgagcagcg
    15541 ggttggggat cgatgtctta ggtttagatt cactgttgaa aaatctaagt ggtggacaac
    15601 gttcaaaggt gattttagca aaactacttt tagaaaaccc agatgttctg atcttagatg
    15661 aaccaaccaa tcacttagat gatcagcata tccaatggct gacgcagttt cttcaggatt
    15721 ttgacggtgc gtatatcgtg atttcccatg accaagaatt tcttgatcag atcaccaccc
    15781 atatcgcaga catcgagttt ggaaagatta ctaagtatac tggccatcta aaacaagcat
    15841 tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca atatcatgct caacaaaaac
    15901 aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc cggttcccga tcaacgatgg
    15961 caaaaagccg acaaaaacaa ttagataaaa tcgaacggct gacgcctcca gcttcagcaa
    16021 gtaaagctgt ctttgatttt ccattctcgc ctattgtcac gacattagca gtggaaacaa
    16081 ctgatttagt catcggatat gatcgaccgc tacttgcacc catcaatttg accatccgct
    16141 ttggtgaaaa tattgccatt cgaggcttca acgggattgg aaaatctact ttattaaaaa
    16201 cactgatcgg tgagattgaa aaaataagtg gcgatttcca cttcccagat aacacaaaaa
    16261 tcaattactt ttcacaagat ctgtactggg aaaatccatt agaaacacca ctacaatatt
    16321 taagcagtca atttccaaaa gcgactatca aagaattacg tcgccaacta gctaaagcag
    16381 ggttagttaa tcaattagca agtgaacctc tcactacatt aagtggtggg gaacaaacta
    16441 aggtgaaact tgcacagttg acaatgaacc aaggtaattt actgattctc gatgaaccga
    16501 cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga cgggatacag aaatttccag
    16561 gaacagtaat catcgtctct catgaacaag aattttatca agatcttgtg caacgagtca
    16621 ttgagattga accaaaataa acgtttgatt gaatagttga aaaaaaggag ctgtgacaaa
    16681 agttttgtca caactccttc ttctgaataa atggtggcca attagctgac cttcggtatt
    16741 agctcaaaaa acggcaaaat atgaggagct atttttattt ggctattctt attacttgat
    16801 gcagaacagc ttttcacaac ctcttttcgt ctatttttta ttttcgagat ttctctttcc
    16861 tttaaaaaca actgtttgaa agaagaacgc acatccttga aaaacaagcc cgctaaccgt
    16921 tacacctaac cagccatact gctgccacat tgtcgtccct agcaatgaac cagccgcacc
    16981 tccaataaag tacataaaca taaatacaga cccgtggtgc tagttaattt taatatccta
    17041 aagaatatct caaagttcct tcaaaacgtc gttgtgctcg acttaataga acactgtaag
    17101 ccagtaatat gctttcaaat ctactctcta gcc
//
LOCUS       DACDRN010000049        16986 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDRN010000049 DACDRN010000000
VERSION     DACDRN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.049"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(352..966)
                     /locus_tag="IWJ80_001947"
     CDS             complement(352..966)
                     /locus_tag="IWJ80_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9461658.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1572..1949)
                     /locus_tag="IWJ80_001948"
     CDS             complement(1572..1949)
                     /locus_tag="IWJ80_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461659.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2249..3130)
                     /gene="galU"
                     /locus_tag="IWJ80_001949"
     CDS             complement(2249..3130)
                     /gene="galU"
                     /locus_tag="IWJ80_001949"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9461660.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3173..4192)
                     /locus_tag="IWJ80_001950"
     CDS             complement(3173..4192)
                     /locus_tag="IWJ80_001950"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9461661.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4825..5829)
                     /locus_tag="IWJ80_001951"
     CDS             complement(4825..5829)
                     /locus_tag="IWJ80_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9461662.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5842..6891)
                     /locus_tag="IWJ80_001952"
     CDS             complement(5842..6891)
                     /locus_tag="IWJ80_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9461663.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6908..7729)
                     /locus_tag="IWJ80_001953"
     CDS             complement(6908..7729)
                     /locus_tag="IWJ80_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9461664.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7716..8495)
                     /locus_tag="IWJ80_001954"
     CDS             complement(7716..8495)
                     /locus_tag="IWJ80_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9461665.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8512..8982)
                     /locus_tag="IWJ80_001955"
     CDS             complement(8512..8982)
                     /locus_tag="IWJ80_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9461666.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(8994..9413)
                     /locus_tag="IWJ80_001956"
     CDS             complement(8994..9413)
                     /locus_tag="IWJ80_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP9461667.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9494..12262)
                     /locus_tag="IWJ80_001957"
     CDS             complement(9494..12262)
                     /locus_tag="IWJ80_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9461668.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            12462..12686
                     /locus_tag="IWJ80_001958"
     CDS             12462..12686
                     /locus_tag="IWJ80_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461669.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            13465..15180
                     /locus_tag="IWJ80_001959"
     CDS             13465..15180
                     /locus_tag="IWJ80_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9461670.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            15183..16946
                     /locus_tag="IWJ80_001960"
     CDS             15183..16946
                     /locus_tag="IWJ80_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9461671.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         5018 a         3487 c         2890 g         5591 t
ORIGIN      
        1 atgtactata attactttgt aaagagtttt ggggaagact ctttcatcaa cgtgtattaa
       61 gagacaagaa agaatccata tggattcttt cttgtctctt tttccactac tttttatttt
      121 tctgacacta catattctta taactcattt ctcataactg tgaaacaact tataaaaata
      181 acaaacttta aacaaaacaa gcttaaaatc aaagatgatc catcttaaaa taactaaaac
      241 aaaaaaagta gtcgttgtta atcctcatta ataatcgttt tctttttttc aaacgctcca
      301 tttttatgtg gaacattatt tttttaagga actttgtcaa catgtggata actattttcc
      361 acttgtgtcc tggactagct gttttgctgt aaaatacaaa acttgctgca gcaaaaagat
      421 cgttgcaaga gaggctgttc cgcagatcgc tggatctaga tttggcaaag tcaagactgc
      481 atcatgtaaa ttggcgtggt tgtccaaaac caaatcagca aattcaaata atcgcaaacc
      541 actgctatgt tgcgatttga tacttcgtga caaatcaaat ccagtaacaa tgattaatgg
      601 atgaccattt tctttgatcc actgagctag ttcgatgatt gccgggtttc ttccttcatt
      661 cgaaagaaga agaaaaatgt cttctgattt gacagaaagc tgatcaataa ttgcctgtcc
      721 tgcaccttct agcgtttcat agacacctcc ggctggattt tcaatcaata tacttggtac
      781 aagatccggg gtttgatccc aaaattcaaa tgctacacca cttagtgtgc ggctagcata
      841 tatctgcaat ctaccacctt gattaatttg tttcgcgatc caagtgccag cttgaagcat
      901 cagcggctct tctatcctta atttctcttc caattggtga taatagtcaa aataacataa
      961 actcatttat ctctcccgcc tagctatact tgacatgaat agaaacagct gcctaatcta
     1021 aggaagtaca taaaaaaaca gtagttagac agtaacacat gtggtctttg ctgtttcaaa
     1081 cacaaaaaag gcattgaacg aatacgccca ataccttgac atagcaaata aaacaacgtg
     1141 cagaccatgg aaaatatcta cataggggta gaattttcac cctagattag gtacaataaa
     1201 ttaggtcgat gcactgcatt gactatggca aaatgatcgg tcagggtgta tgtgtgtgac
     1261 tggtcattcc ctgattctta gcgccaacta agggtcaggg agcctttttt tattcagctg
     1321 tttctatctg aatttattgt aagtaatctc ttatccaagt gcaagcaaga aaatacaaaa
     1381 aaacattggt atgatttata tatatataaa taacataaaa aaaggaatgg cttatcccga
     1441 tacaacaagc attcctttaa aaaagattct tttatataag tgaaaaaagc ttttttccac
     1501 caattaaata aatctctcat ctcaatagtt tatccattat tttatctttt tttgcgtata
     1561 cctactaaat attaccgtct tcttctcgtt cttctacttt gttcgagaga atctgtacta
     1621 gaaaaagtag ttgtatgtct accttttctg cctatccgag actttctttt catatatggg
     1681 atataaatga ttagatacag tacagcaaaa aataaagcaa atacgcaagc ttcccaacta
     1741 cctaacgcca attcaaccat caaggtagca accccaacgg atgctgcata ccagataaaa
     1801 atcagaaacg tttctttttt tctgaccatg cgaaacatga taaatagact aataagcaat
     1861 agtggcaaca caaatatcca attgcttcct tccatcagcc acttgagtat atcaagcagc
     1921 gactgtgcaa cagcgactaa ttttcccata gtgaacacac accttttttt ataattaaaa
     1981 aattcgcctt tttattctac tatgaatctt ctggcttggc tttcatttct cttatgtaat
     2041 cattttcttt tatacaactt aaaaaaaaat taaaccatta gcataatatt ttctgaatct
     2101 attcacgaaa gtctttttct atctttcgtt ttccacgact caactgatct agagtcaaaa
     2161 aactaaaacg acagttgcgc aaatgacgca caagctgccg ctttaataac aaatagcctt
     2221 tcattctcag caggccactt atttccagtt aactttctgt tcttagcttt ttactcaact
     2281 ggataatata tgcacttaat gaagtcccaa tttccgaatg ttgaagtcca tattcaatat
     2341 ttgtcttcat atatcctaac ttatcgccta cgtcgtatct ttttccttta aactctctcg
     2401 catagaccgc ctctgtctgg ttaagagtct gaagtgcatc cgttagctgt atctcgtttc
     2461 ctgctcctgg ttcttgagtt tctaatatct caaatatttt aggagtcagt agataacgtc
     2521 cgataatggc taaattactt ggtgcttttc ccatttcagg tttttctaca aaccctttta
     2581 ctctgcaacg acccttttcg ctatctgttt taggatcaat aattccgtat ttagatgttt
     2641 cattttctgg tacacgcatg acggcaacag cggaagcttg tgtttcttca taatccatca
     2701 tcaattgttg agtcaatggg atatcatctt tcatcaaatc atcccccagc atgacgacaa
     2761 acggttcatt tcctataaat gcttttgctt gaagcacggc atcccccagt cctcttggat
     2821 gagattgacg gatgaagtga agattgactt gagtcgtttc ttcaactaaa gcgagtaatt
     2881 ctgttttttc cttttcccgc aaattgtttt ccaattcgat atttgcatca aagtgatctt
     2941 caatgggtct tttttctttt cccgttacga tcaagatgtc ttcgattccc gaatccaatg
     3001 cttcttccac aataaactgg atcgttggtt tatccacgat tggaagcatt tcttttgcca
     3061 tggcttttgt tgccggcaaa aaacgtgttc ctagcccagc ggcaggaatg actgcttttt
     3121 tgaccttcac ttgctgttcc tcctaaacga cgattaacta ttgatcatct atttaacgaa
     3181 gagaaaattc attctccatt tttccgtcac gtaacatgat ttcttttgct gcttttttga
     3241 tatcttctcc attatacaaa acattataga ttgtttgagt aattggcatc tcaacaccta
     3301 gctgttgagc tagctctact gctgcttttg tcgttgccac accctcaacg accattccca
     3361 tgttttctag aacttcttcc agcttatgtc cctgtcctaa caagttaccg gcacgccaat
     3421 tgcgagaatg aacacttgta caagtaacga tcaagtcccc cacaccactc aaaccgataa
     3481 atgtcagagg attggcaccc atcgcgacac caagacggct gatttctgcc aatcctcgag
     3541 tcatgattgc tgcttttgca tcatctccga atcctaatcc atggatagcg ccggcaccaa
     3601 ttgcaatgat atttttcagt gctgcaccag tttctacgcc aatcacatca ttatttgtat
     3661 aaatcctgaa ataatcattc atgaacagtt tttgtacata ggctgctgct ttttgatcaa
     3721 tacttgcagc tgtaattgtt gtgatatcat gtactgccac ttcctcagca tggctaggac
     3781 cagataatac aacaattgcc tgtctttttt cttctggaat ttcttcagcc aaaacttctg
     3841 agattcgttt atgcgtatct tgttctagtc ctttacttgc atgtataatg attggcttag
     3901 tattcatttt accaataagt tcctgtgcga ctgaacgaat cgcttttgtt ggaacaacaa
     3961 ataaaatagc atctgcgtta tccactgctt ctgccaattc tttatatccg ataatcgatt
     4021 ctgggatcgt caaatcaggt aaaaaatgct gatttgtatg atacttattg atttcatcaa
     4081 tctgttcagg tacatttccc caaatacgga catcatgccc gttttctgcc aatgtttgtg
     4141 ctaaagcagt gccccaagat ccaggaccaa gaactgcgat tttttgtttc atggatattc
     4201 ctccaaataa ataattgata atttccatta tagtatacat ttcttaaagt tgggactaat
     4261 ttccattgtt tttaagtgtt tgtttacttc ttttatcgtt tttacttgaa ataatcaatg
     4321 atatgttaca atagtctaca ttttattttt acactcgtta tgctttgctt gttcctaaga
     4381 ggaagcagtt ttgacaatgt tactaccagc attactatta ggaggttagg aaacttctcc
     4441 tagtgagtat tgatttaaaa tacataagag acagattgaa catttctaaa tctgtcaggt
     4501 cctgcaacgg acagattgtc tctgttgacg gttgacatag gaacttttgt tttagtcaac
     4561 attctggata caaaacgcta tgtaaactgg cgcaactcgt tgtacttgga cttttactac
     4621 ggatccatgc aacaactaaa tagtactatt gcataacaaa aaatggttgg cacccacatg
     4681 tcccattaaa cagattataa gttgaactgt agataatgca aaaaatgtgt tttataacgt
     4741 aattgatgcc aatattttaa aaaaataaag ggagcagtta tcgctgctct ctttattttt
     4801 tatattcttt ttatccagtc tatcctaata atcaaactga cgatagtagc ggcgtatctc
     4861 taatggatgg cagttcaact gctccacatg agcatcgatg cgttgtgtca ctacatgcat
     4921 caataagaaa gacaaaacac gtccgcgata tttttcactg ataccttcta ctggatagtc
     4981 ttttgtgtct atcaatgtat aattgctgca aatttttggc aaaagtttag ccacacgttc
     5041 agataacact ctctgttcat cttcgcctaa aaataatgtg actggggttg tttcttctac
     5101 gatctctaac gtcccgtgaa gaaactcggc agcatggata gatttcgatg gaagccagct
     5161 ttgttcttcc cagtaacaca tggcatatga ataaacagct ccccattgat tccctgctcc
     5221 aataaaataa tgcatactat cacgtcgatg tttttctgca aatgataagc caaatgcatc
     5281 tgcttttttc tctgcttcca ctaaccctgt tggtaaataa cgttcgagct gttcatagta
     5341 ttctgaataa gcttcgaatt ctccattcaa atacatcaat cgatctgctg ccataaaaaa
     5401 tttgatttgt tctgttccag gagctggata agtgaccaca aaatcgcata gctgagacaa
     5461 cttactgttc gctttatcga taaatccaat cagtgtcgct cctactttct tgatttgttc
     5521 tactgctttc actacttctt gagttgtacc ggtaacagag gacaaaataa cgatagaatc
     5581 ttttgttaat cttttgtttc cagtagtgta atattctgca gcatgctgta caaagacagg
     5641 caagttgctt ttcccattca tataagtgac agcttgcatc gaggaagcat acgttccacc
     5701 gattcctaga taatagacag cgtcaaattt ttccgcataa agtttatcga tgatttcttc
     5761 tacttgcgga cgtaaagcta gtgcgccttt tattgctttg atttgttctt cttcattgaa
     5821 tttcagcatg atttccacct cttataattt acttttcaag actgtatcaa agtcttcaaa
     5881 aatctttttg ttcaagtcaa tacctttagc tactgcggtt ttataagcga atagttgaaa
     5941 cggaacgaca agcgctaata cagacagata ttcatcacat tctattgcta atccaagtgt
     6001 tttctcgttt cttgcttttt ttgtggtaat ggtgagagtc tctccaacgt accgactcat
     6061 atatctttgc aacttttgcg aacgttcctt atttacactt tccccttcaa taaagaatag
     6121 caaatgcgaa gcatctgcct ccaggtatgg gccgtgcata taagcttcta actcaaatcc
     6181 ttgtgacgga acgcggattg tttcagtcaa tttcgtctct gcttctttag ctgttcccca
     6241 gtttggacca tatccaatgg tgacgaaacg ctgtgctaaa cgaaataaac tttgatatcc
     6301 atcaaaaaat tcttccgttt tttgaataat cgctggtaaa tggttgatta ttttgcgcaa
     6361 ctgttgcatg tagtcttgtt ctattttttt gctgatttta tttttactta ttccaattcc
     6421 tatccctaag agtagtaact gtaaaacagt cgcagaatag ccttttgtaa cgaagcctac
     6481 tgattctacc ccggcaccta aatcaataat ttgtgcagct tctaaagcaa gtggactttg
     6541 cacgtcattt gtgatagcaa caacaggtaa cccttgtttt tgtatcattt ttactgcttc
     6601 aacagtagaa gcacttttcc cgctttgtga cacagcaatc acagtatcga ttgaaggaga
     6661 aagttttcca taattgaaaa aataaaaagg ttcttgaata tcgacagtaa gatcaccata
     6721 agattccaat gctggcttcg ctgctaaaca agcgttataa gatgaacccg ttgctaagat
     6781 cagtaaattc actgtttttt tcatatgttc tcttgtcgaa aaatcattct ttttcaagat
     6841 cgtacttagt acttcctttt cttcatagat ataatcttgc atcgtaaaca ttttctctgc
     6901 tcctttttta aaaaatccct atccacgaac caaaaattcc aaagaaagct agtccgatca
     6961 agatcgttaa tggtttcact tctttcttca atagataaaa cactatgcca aatgctccaa
     7021 gacataagat atttggaacg atatcatcaa aaatactttg aacagtcaca gcattttctc
     7081 ctgaaccgat ttttaaagga atattgatgg tgatcatcgt ggcagtcatt gctccaacga
     7141 ccatcagtcc aataatagac gcgcctaatg tcaaactttc catcataccg tttgcctgga
     7201 tctttttcaa aaaacccgtt cctaagcgat atccccaacc tgttgctaga tagcgaaaca
     7261 gaatgtgcgg aatattgaaa atcaaaatga acaagatagg gcctaaaata tttccttgca
     7321 tggctaaaga ggtacccaca cctgtggcaa tcaatcgcaa agttccccaa aagaaagaat
     7381 ctcctaaccc tgcaagaggt cccatcaaag atgttttgat actatcgatc gcagtcacat
     7441 caaattcagg atcatcctta ttttcttcct ccattgcagc cgtgattcct aaaatcagtg
     7501 taacgatatg tggggtcgtg ttaaaaaact ctagatgccg tttcaaagct gctgccattt
     7561 cttctttcga ctgatataac tttttcaaaa tcggaatcat tgcataaaca taagccatat
     7621 tcatctgacg ttcataattc caagaaaact ccatttgaaa agaacgccaa aatacgtgat
     7681 ttagctcttt tttcgttatt ttgctgtttt tgatctcaga attcttcatc ttcttctacc
     7741 tcccctgcag ttgtttgaag tggtagttga cggacattca taatattgac taccacaact
     7801 gctaaaatcg caccaaaaat agctactccg gtcacaggaa tctctaagta agaagctata
     7861 gcaaaaccta agaaaaaata tggtgcaact tgtttgttga tcagcaaacg agcaagcatc
     7921 gcaaaaccaa gagctggaat caaccctgta gctactgaca accccttttg aacgaagtca
     7981 ggaatcatat ttaatagatt tccgatcgta ttgcttccca ccatataaga aaccattacc
     8041 acaacaccaa gcatcaagga aagaccgata ccggcaagca agtgcatatg ctcgactcct
     8101 ttataatttc cttcttctgc ataattgtct gctttatggt tcataattgg aatcaaaagc
     8161 cctaagtaaa tatttttcaa tatcaatgtc aacgtagcaa tcggcaaacc tagtaaaagc
     8221 gcggtttctg taccggctcc agctgtaata gcaaaagccg tccctaaaat cccaccggtc
     8281 acaacatcag gtggaatcga agcacctact gagaacgatc cgataaatgc taactctaaa
     8341 gttgcgccca taatgatccc agtttttaca tcacctaaca cgattcctgt aaacagcccc
     8401 gtcacaatcg gacgagaaag caacgatgtc cctagtgcat attctgattg tgcgacaaat
     8461 gcaacaattc ctaataagac tgcttgtaca atcattgagg ttcttccttt cttacaaaac
     8521 ttctcttaca tccacttttt tatctgctgg tacttgacgg atttctactt ctatttgttt
     8581 gtcaattaat cgtttcaagc gttcctcctc ttctggtaaa atatttaccg cttttccgat
     8641 gctgcgagta ccaggttttg ccttgatccc tcctaagttg atggttttga tttctggata
     8701 cgcttctgcc agcttatatg catcctcgat cgattccact acgatgaata acttatattt
     8761 atctgtcacc ccgcttttca acgcggcaat cgagtcatca attgatttga tcactagttt
     8821 gactccttgt ggcttggcta atttgatcgt tgtctttcgt atctcattcg taggtacatc
     8881 atcattagca atcaaaatac agtctgcgcc tattgtttgt gtccatgaaa aagctacttg
     8941 tccgtgtaat aaccgatggt cgactcttgt caatacaatc atttttcttc ctcctaaaat
     9001 tcgtcttctt ctattttctg attgagccgc tcgttacaaa actggatcat tttttttgaa
     9061 tcagttagca cctgctggat caaaacagtt gtctctactt cagcgtccaa ccgagatact
     9121 agctcaatca atagtggcaa attcaatccg gcaacgagat agacatgatc ccgattgata
     9181 tattgtaaga attcgttgtt gacacttccg ccaaaaagat ccgtcagaac aatgatttct
     9241 ttatctttgt acctcataaa aagctgcctt actgtttccg ctaaatcaaa attctcatca
     9301 gtatagcaat cgaatacagt aatagaatga tttttcccat agatcagctc taatgaatct
     9361 aatatccctg aagcaaattt tccatgggaa gctaagataa tttctctatc cactttttca
     9421 tctcctcttt tcacttacac atactaaaaa agcaagttgc atgccaaaat tagtggcaac
     9481 aacttgctta tgttcaaaat tcttcttcta tcgagagaaa ctctgtatct tcactcaaaa
     9541 tatcatagat ataggcaatc tcagaattgg ggatcatgac actataatgc tcttcaatga
     9601 cactaaacgc agatttaata gcagataacc cttctttttg acactgcttc catttctcta
     9661 atccttgata ggtttcgatt ggcatttgtc ggatcaatcg ttcaatgaga cagctcacat
     9721 ggacatacaa cgctaatttt ttgggattgc tgatgcttcg attcaactgc acctctaatt
     9781 ctctcataaa atgatcgatt tctcccatca ccttctctgt atctagaata gtcacggaat
     9841 ccagtacttt ttccaatgaa aaattacgaa tgatgcgctg attgaatatt tccttctctg
     9901 aagcattcat agtagtgtcc aaccattcag ctagtacaga catcttttcc cccgagatga
     9961 ggtcttctaa tgaaagatag tcgatattgg tgatatcagg atctgtggtg ccaatcattc
    10021 ctacgacttc gtatatggaa aataatgggt cactgttttt cttatctttt aattgctgat
    10081 attcatatgg gatgattttc aactgacaag ccgtaggtaa gcttttttct agcagttcac
    10141 ttaagtggac cgccgtaccg attccagtga agcaagtagt aatgagtgct ttgggtttgt
    10201 tttcctctgg atagagcagt cgccattctg gctggatctg tcgaacagtc tcttctgcta
    10261 tctcttctaa tgatacattt ttttgcaggc attcccctac tgtgattgct aaaggtgtcg
    10321 tcacattatt caaaatcaaa agcggtacgg ataactgttt tttaaaaaac tgatggattt
    10381 ctttcagaga tcccatgtct actaatatga tcaatccatt tgaaacatcg ctgttttcgc
    10441 aataatcaag gatttctgaa gcaatctgct gtggtgtgac atctagtggc atatcaaatg
    10501 attcaaatag atcctcttgt aataaacgat ttactacatt tgccatactg ctagcagttg
    10561 cataaccgtg agcagcaatc atcgcttttg gcagtcgttt ttcttttgac catttcgctt
    10621 tttttagata gatcgtcagt aaaatccgat ccatacttgt gtcttcaata tctaatttga
    10681 tcttacttaa ttccagaatt cgttggacat agtgataact agatggataa gactgagaaa
    10741 cttgttgttc taattctttg attagttgga taatatcatg ttcttccggt aaccatttga
    10801 tagtgcttcg ctgatagaga tagtaactca aggcatagac actgtttcca tcgaatttga
    10861 tttgataggc agtttccatt tgtcgaaagg tctctcggat agattgtgtc caataaacca
    10921 gcagctcgtg tttttcttgt ctactggtct caaacagcaa aaaatcaaat aattgaatca
    10981 cttcttgctt taattttcct tcgcaagaat atagcagaca gttatttttc ttgaaatcaa
    11041 ttaggatttg ctcaaaactt ttaacgattc gctgctgttc gggctgttgc ttctccatta
    11101 attgctctaa ggtctggtct tgcgtaatat agattccttg ctgtgctgta tttttagtaa
    11161 taggctgcgc aagtagttct ttttgcaaat gatggatcgt gacatggatt tcaggctgtt
    11221 cacgctgttc agcatatgct tttgctgctg aaactttgac gatatttttc agctctccta
    11281 tgtttccgga taatttctga cttgttaaaa gtgacaaagc tggtccactg actgacaaag
    11341 ggctccccat ttttctttgt tcttcgagaa aaaaagagta aatcagttct agccgttcat
    11401 tacgactacg ctgattaagt gcaggtagtt cgatacggat aggtattctt cgaatgaaag
    11461 ttgttaagaa atgactagta atctcttcag tcgtagcaaa aaagagacgt gttttgatcc
    11521 ggcgtgacaa attaggttct cccatacgat aaatcacacc ttgatcaaga taggtgaaca
    11581 gtttttcctg accttctggt gttaagcgat gtacctcgtc taaaaacaag atcccaccat
    11641 ctgctgcttc aaaagcccct tttttatttt cttctgcacc agtgtacgct cccttaacat
    11701 gtccaaacaa attgctagtc aaaagttcag gattatctgc atattgtgca caattaaaag
    11761 tgatgaatgg tgcagaatct tctagcagat catgaagcag acaatactga tagaccaaat
    11821 taactaaata acttttacct gtgccacttt cgcctgtaat caaaagtggt aatccgccat
    11881 ctggatagta aagagctgtt ttgatttgtt cgatactttc tttcaaactg gcgtcgtatc
    11941 cgatcataaa agagaataag tcttttggtt gcgcgaaaaa aggctgttcc tctttgagat
    12001 catcgataga aaaataaaaa ttcgtgcgta acttaaaaaa ttgtttttca aaagcttctt
    12061 tgtggaaata aatcactggt cgagaattga ttttgactag tagttgctca tcatttaact
    12121 ggttcaaata atgactgact gtgtttcttt ttaccccaaa atgatcagct aacctttgtg
    12181 ctgtaaagac ctcgttcata tgttctaaat cgaaaaaggc tgtctggttc tctaagtaac
    12241 ataaaatttc cttcttcaac aacttattca cccctcctcc atcatagaaa acggattcat
    12301 tttgtcaata aaatctttaa taaagaattg aaaaatatat cttattatag acagcagcct
    12361 acttatccct tataattttt attctaaaaa acgtacagtt ccttccagtt tttatactgg
    12421 aatttcaaca tatgaaagaa taaggaggct atgactattc aatgaaatcg gataaatata
    12481 aaaacaggaa acatacttcc tggaaaactt ttcgagaaat catcggatct tatctcgctg
    12541 tagttgtatt tttgcctttg ctttatatgg ctatgacaga acaaactcgt tttattggcg
    12601 tagatacgca atatatagcc gaaacaggat ttttattcat ctttatcttg atttttgcgt
    12661 tctataaatc cttccataca aaataagatt cacagatttc aactttcctg tctgttttta
    12721 gctattacaa atccttatat gtggtataac aacaatttat tggcataatg aggaaaaacg
    12781 agttatgatg ctttcagaga gtaaagaggt actgttgtca ccatcctttt tattgattac
    12841 gtacaccact cttagttttt tcccattgat tacgaaaaca aggtagaaac attgatgttc
    12901 ctaccttgtt ttcttttttt attttcagat caaaatctca agtagctttt gacttgatgt
    12961 cgggaggttg tcttcgttcc atcaaacctg atagaatctg cgccaaacca tagttttctc
    13021 cactattatt tattcctcta agatagtacc cacaataaac gaataaatat ttttctcatt
    13081 ttcatactcc ttctttgaaa atcaattaga tttctctcaa cccacttttt tacaaaaatt
    13141 acgtagcaaa aaagattgca agaacaaatt aaaaaagaag taaatagagt ttcatctata
    13201 aacatttact tgatatattt tttatgcaga attcatttat agaaaaatta agtaaaatag
    13261 acgttcctat gaatgaagag actggcatgt tttctcattt tctttttttt aaaaaaaata
    13321 gtctattcaa tttctaaagc ttcttcataa taaaactttc atttatgaaa aggcttgttc
    13381 tcttttcatt ctaaatttcc taagctacaa tgtggtagta cttgtttttt caattgtgga
    13441 caaataaata ataggaggaa tcatatgatt cagttattac gttatgcaaa agactatcgt
    13501 aagcaaatca ttttgggccc gttcttcaaa tttcttgaag ctgtcttcga acttgtcctg
    13561 cctttaatga tggcttcctt gatcgataat ggactaaaaa tgaatgaccg aggaaaaatc
    13621 attgaaatgg gcttatggat ggtcgctatg tctgtgattg gtctgatttg tgctatcatt
    13681 tgccaatact atgcttcagt agcttcacaa ggatttggta cagagttacg taatcagcta
    13741 atcaaaaaaa tcaatacttt ctctcacaac gagttgaatc attttggaac agataccttg
    13801 atcacccgaa tgacaaacga catcaatcag cttcagctag ctttagcaat ggtcattcgt
    13861 ttattgatca gagcaccgtt tttaagtatc gggtcagtgg tcatggcttt tgtgatcgac
    13921 tgggaagtag gtctgttttt cttagcatta ttgcctattt tctcaatcat tttatttttt
    13981 atcatcaaaa aaaccgttcc tctctaccaa aaagtgcaag aaaaactaga tcagctcaat
    14041 gaacacgtta gtcagaatct tagcggtgtt cgtgtcatcc gcgcatttgc gcagacaaaa
    14101 aaagaaacag agaagttcga taaaagcaca aatgatctag cgaacaatta tttacgtgtt
    14161 tccaatatct ctgccctatt atcacctgct acaaccttga tcatgaatgt cgggatcatc
    14221 tgtttgctga cagttggcgg gatcaaagta aatattggta gcttgcaaca aggacaagtt
    14281 cttgctttga tcaattatat gaatcaaatg cttttggcac tgatcgttgt ttcaaacctc
    14341 gtcgtgatct tcacacgtgc cgaagcttca ggaaatcggg tgaaagaggt gctagacaca
    14401 gaaaattcga ttcttgaagc tgaggtcagt actactcccg atttttcttc tgaagcaatt
    14461 atcgcttttg accatgttga cttccgttat actcctgatt ctggtctttc tctccaaaat
    14521 attacatttc agctgaagcg aaatacagtc cttggaatca ttggtccaac cggaagcggg
    14581 aaaacgactt tgacacaatt gattccgcgt ttctatgatg tcagttccgg tacagttata
    14641 atggacggaa tggatgttcg ttcttggcag ctggatcatc tccgtcagca aattgctctt
    14701 gttccccaaa cttctgtact atttaccggg actattcgtg aaaaccttca atggggtaag
    14761 aaagacgcca cagaaaaaga atgttgggaa gctctgaaaa tcgctcaggc agaagaattt
    14821 gtccgtcagc ttccaaatgg tttagataca aaagtcatgg aaaatgggaa aaatttctct
    14881 ggcggacaaa aacagcgact gacgattgca agagctttga ttgccaagcc agcattactg
    14941 attttagatg actctttaag tgcgttggat tatcaaacag acttggattt gcgtaaggcg
    15001 ctacagactt atcttggcag tacagtgatt attatctccc aacgtgtccg ctctattcaa
    15061 gaagcacatc atatcttagt gatggatcaa ggtaagattg ctgctcaggg tacccatgaa
    15121 gaattacttg ctcaatcagc agaatatcag gaaatcgtcg catcacagga ggaagaatag
    15181 ttatgaaagt aaaaaaatta tccttgcaga cgctccgccg attcattcct taccttactg
    15241 cctatgcaaa agacatttgg ctagctgtga tactaggaat catcaatggg attgcaacag
    15301 ttgtgatgac gttacaaatt ggtcaaagta tcgatcaaat gattggagaa ggacaggtcc
    15361 gcttcaatcg actgttccat ttacttattc tattcggtgg tatcgtttta atcaacgtag
    15421 tcagtcaatg gctcatccaa gtgttaagca atcggttagc ttacttatcc gttgcaaaat
    15481 taagaaaaga tgcatttgct catctgaatc gattgccact tcgttattat gatcaaaact
    15541 cccacggaaa tatcgtcagt cgatttacaa atgatatgga caatatttcc attgcaattt
    15601 ctgcggcctt caatcaatta ttttctggag tggcaatcat cattctttcg tttattttta
    15661 tgatgcgtct aagtccactt ctgacattgg tcgttctttg ttctacacca attattttcc
    15721 ttgtgagttg gatcgttgct cgtgcttcac aaacaaattt cgatagacag caaaggatta
    15781 ttggggatat ctcgggcttt gtgaccgaac gaatcggtaa ccagaaaatc gtgaaagctt
    15841 ttcagcaaga agaagctagt caacagcaat ttgaactgct aaatgctgat ctatatgaaa
    15901 aaggacaacg ggcacaattt tcttcttcct tgacaaaccc gtcttctcgt tttgtagatc
    15961 accttgctta tttatcgatt ggtttagttg gcggcctatt ggcattgaag agtggttcgt
    16021 caatcactgt tggtgtgatt tccagcttta cgatctactc aagtcagttt tctaaaccat
    16081 ttatcgaact ttctggaatc acgacacaac tgcaaaccgc tttttctggt ctagaacgag
    16141 catttgattt gatcgatcag ccagaagaaa agcctgattt gccagatgcc tatgccttgg
    16201 aacccaaaaa tatcaaagga aaagtctctt ttgatcatgt ttctttttct tatgacccca
    16261 agcagcgttt gatcgaagat ttcaatttta ctgcttatcc aggtcaaacc attgctatag
    16321 taggaaaaac aggggccgga aaatccacac ttgttaattt gctgatgcgt ttttatgaca
    16381 caacaaaagg aacaattaca gtcgatgacc atgacatccg ctctatccaa cgtgatacat
    16441 tacgtaaaag ttttggaatg gtgttacaag atacttggct atttgacagt acattgcgag
    16501 aaaatcttcg ttttgggaat ccggaagcaa gtgacgcaga aatcaatgat gccctaaaag
    16561 cctcttatat gtatgaattt gtagaacgtt tgccggaaaa attagatact aagattggcg
    16621 gtcaagggtt aaagatatca gatggacaac gccaactgtt gacgattgct cgaacgatga
    16681 tcagtaatcc ccctatgttg atcttagatg aagctaccag ctcagtcgat acattgacag
    16741 agaaaaaaat ccaacaagcc ttcttagcaa tgatggaagg aaaaaccagt tttgtgattg
    16801 cccatcgcct gtccacaatc aaaaatgcgg accagatatt agtgatggat cagggagcta
    16861 ttgtcgaaat gggcacccat gatgagctgc ttcaaaaaga tggttattac cgacggctct
    16921 atcaagcaca attcacagca caataaaaac aagcagtctt ctgggatggg aagactgctt
    16981 gttttt
//
LOCUS       DACDRN010000050        16881 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDRN010000050 DACDRN010000000
VERSION     DACDRN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.050"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(258..1763)
                     /locus_tag="IWJ80_001961"
     CDS             complement(258..1763)
                     /locus_tag="IWJ80_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9461672.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1760..2446)
                     /locus_tag="IWJ80_001962"
     CDS             complement(1760..2446)
                     /locus_tag="IWJ80_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9461673.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2639..4060)
                     /gene="gndA"
                     /locus_tag="IWJ80_001963"
     CDS             complement(2639..4060)
                     /gene="gndA"
                     /locus_tag="IWJ80_001963"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP9461674.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4230..4409)
                     /gene="rpmF"
                     /locus_tag="IWJ80_001964"
     CDS             complement(4230..4409)
                     /gene="rpmF"
                     /locus_tag="IWJ80_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9461675.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4467..5036)
                     /locus_tag="IWJ80_001965"
     CDS             complement(4467..5036)
                     /locus_tag="IWJ80_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP9461676.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5222..6124
                     /locus_tag="IWJ80_001966"
     CDS             5222..6124
                     /locus_tag="IWJ80_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9461677.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6159..7943)
                     /gene="pyk"
                     /locus_tag="IWJ80_001967"
     CDS             complement(6159..7943)
                     /gene="pyk"
                     /locus_tag="IWJ80_001967"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP9461678.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8017..8979)
                     /gene="pfkA"
                     /locus_tag="IWJ80_001968"
     CDS             complement(8017..8979)
                     /gene="pfkA"
                     /locus_tag="IWJ80_001968"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP9461679.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9147..12461)
                     /gene="dnaE"
                     /locus_tag="IWJ80_001969"
     CDS             complement(9147..12461)
                     /gene="dnaE"
                     /locus_tag="IWJ80_001969"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP9461680.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12692..12877
                     /locus_tag="IWJ80_001970"
     CDS             12692..12877
                     /locus_tag="IWJ80_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP9461681.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(12939..14135)
                     /locus_tag="IWJ80_001971"
     CDS             complement(12939..14135)
                     /locus_tag="IWJ80_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9461682.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14305..15741)
                     /locus_tag="IWJ80_001972"
     CDS             complement(14305..15741)
                     /locus_tag="IWJ80_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9461683.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            16034..16843
                     /locus_tag="IWJ80_001973"
     CDS             16034..16843
                     /locus_tag="IWJ80_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9461684.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         4954 a         3539 c         2950 g         5438 t
ORIGIN      
        1 aaataataat atagcaatct ctttataaaa ttgcaataga aaattcccta tttttattta
       61 ttatttttaa acacgaatat tacagcattt tattgttttt tcgttcatct aaaagatact
      121 tacatttaat taacggatgt tattctattt aattgttcgc tagttttttt ataatgacat
      181 ccctgaattt ctcacttgct tgtattttgt accttattct ttgttggata tataaaaaaa
      241 caagtaaagc acatgcttta cttgtcttct gcctttgcga taggaataaa gattctaaaa
      301 attgtccctt gccctactac actttctgct aagatacgtc ctttatagtt ttcaaccagt
      361 tgtttagcga tagacaaccc cagaccattt ccacccttcg ttctagctct tgctttatcc
      421 actcgataaa aacgattgaa gatcttatta aggtcttctt tgggtattcc ttcgccaaag
      481 tcctgaatcg cgatctcaaa ttcattcaga gtggaagaaa tagagatatg gacttctttt
      541 cgtgtagttg aatactttac tgcgttatcc aatatgataa tgattaattg ttcaaagtga
      601 ttacgataaa tttgcagggt cacttctttt gtcaaatcat catctaaagt gatcacatag
      661 tctggatata atatcttgaa attattataa acttgatagg tgacttcttt tcctttagtt
      721 gtttcattcg cataatgaac atctacttgt tctgctcttg ataagtctag catctcttga
      781 actaagcttt tcatacggct gatttcttgc atgctggcgc ttaatgattc ttctaatatc
      841 tcaggatcat ctttccccca acgatttagc agactaagat gcccttcaat aattgcaaca
      901 ggtgtcctta actcatgaga tacgtcttcc acgaattgct cttgttgttc aatatacaaa
      961 cgcatacggt caagcatctc attgaatatt tcagctaaat ctgccaattc atcatttgtg
     1021 tcgatttcag gcatgtggat atctgattgc ggatctttac gtatagtatc cattgtatct
     1081 ctgagtacct tgagtggttt taagaaataa gaagataaaa agaaacctaa aatactactt
     1141 aagatcaaag aaacaacttc taaaacaata agagtcaata atagcttatt tcgaatatca
     1201 tagaaagaag ataattcata gaagctttgc gcatagccga tcttttctct tgtaccttta
     1261 gaatatatag gctggataga taaaaagcca gtttttcctt ctagtgtaac gatcgttgga
     1321 actttacgtg tagtctgaat caggtttcga ctgttttcat gggttttaaa aatcaagttc
     1381 tcgtccaaat cataaacata aagatccatt gatggctgcc ctagctcagc tataaaggaa
     1441 tcgatcttca ttaagtttcc ttcgaccagt gaagtatgat ctacgacctc atctccgtca
     1501 tttgtcgctt tcgtcagatt acgatacgta tttactaatg tcagttcttc atccgagtta
     1561 gccaaacgag aagctacttc tgaaatcgta cgctctacat tgtttctttc ttttgccaca
     1621 atcagattga tagatgattt ataggtaatg acagcgaaaa ttgtaaacac aacgaatatg
     1681 aagaaagaac tcgcaaaggc ccacttgatt gtcaaggagg gccctttcag ttctcttttt
     1741 tccgattttt tcggctttat cacgagcgca tcacataacc tgttccgcgt acagtttgga
     1801 tatagctttc ctccccggga acgtcaattt tgttgcgaag ataacgaata tatacgtcca
     1861 ctacgtttgt ttcaacttct gtttcgtatc cccacacttt atttagtaaa acgtcacgtg
     1921 ctaataccac gttcacgttt tccattaaag taagcagcaa ctcgtattct ctttttgtta
     1981 gttcaattac ttcatttccg cgacgtacga cacggttttc tttttcgata gtcaaatcgc
     2041 gataagtaat agtagtttgt ttagccacat ttttatcgcc ttcaatatcg attcgacgca
     2101 acaatgcacg aagacgtgct aataattctt cgatagcaaa tggtttgacg atgtagtcat
     2161 ctgctccgtg gtcaagtcca gatacacgat cgatcacaga atctcttgca gtcatcataa
     2221 tgattggtgt atttttgact tggcgtacac gtctgcagac ttctaaaccg ttcaattctg
     2281 ggagcatcag atccaacaat atggcatccc actcattatt taatgccgca tctaaaccag
     2341 ttcggccgtt gtagtggact tctgcgttat acccttcatg cttcaactct agctcgacaa
     2401 atcgtgccaa gttcttttca tcttcaatga ttagaatatt gctcatttat tattttatcc
     2461 tttctattct actggctttt taaaaactct atcattatac cgataatatg cttaaaaatc
     2521 tatagtcatt ttattatgga tagcaatttt aaaaaagagc tgaagtctac tcataacaat
     2581 cgacttcagc tctttcgtcc tctttttatt ttctcatttc ctaacagccg aaagactatt
     2641 attcttcatg gtaccatgag taatggaaga ttccttcttt gtcgacacgc tcataggtat
     2701 gtgcgccaaa gtaatcgcgt tgggcttgga ttatatttgc aggcagacgt tctgctctgt
     2761 atgaatcgaa gtaagcgatg gctgatgaga aagtcggtac aggtacgcca gcttggacag
     2821 ccaaggcaac gacgtctctg actgattgct gatatttttt cgtgatttca acgaagtaat
     2881 catctagcaa taaattttcg atctcaggat ttttgtcata ggcatccgta attttttgca
     2941 agaatcgagc tcggatgata catccagcgc gccagatttt tgcgatttca ccaaatggca
     3001 agtcccaatc aaattctttt gaagcgacac gtaattgagc aaatccttgg gcataactca
     3061 tgattttact gaaatacaac gcttctcgga ttttttcgac caattctttt ttgtctcccg
     3121 tgaactcaaa atcgttcgta cgagacaaaa tcttgcttgc ttgtacgcgt tcttctttat
     3181 aagcagaaat atagcgggca aatacagatt cagtgatcaa tggaagtggc acacctaagt
     3241 caagcgcgct ttggcttgtc catttacctg ttcctttatt cccagctgca tcaaggatca
     3301 catctactac tggtttacct gttccttcat cgtctttacg tgtcagaatg tcagcagtga
     3361 tttctatcaa atagctgtct aattcgcctt gattccattc tttaaagata tcagccattt
     3421 cttctactga aaggtttaaa atattcttca ttaagtcata agattcagca atcagttgca
     3481 tatctccata ctcaataccg ttgtgtacca ttttcacata atgtccggct ccatttgggc
     3541 caatatatgt gacacatggt gctccgtctt ctgcttttgc agaaatttgc tctaagattg
     3601 gtgcaactag cgcataggct tctttttgtc caccaggcat gattgaaggt ccttttaaag
     3661 ctccttcttc accaccggaa acgcctgtac cgataaagtt gatgccagag ttggctaact
     3721 cttcattacg acgcatcgta tctttaaaga atgtatttcc tccgtcaatc aaaatatcgc
     3781 ctttatcaag gtgaggaagt aactcttgga ttgttgcatc tgttgccggt cctgctttga
     3841 ccattagcaa gatccgacgt ggtttctcga tagattctac aaattcttcg attgtatatg
     3901 ttgctttgaa atttttatct gggtgttctt ctactacagc ggttgtttta gcccctgtac
     3961 gattgaataa agccactgaa tagccgcggc tttctatgtt caaggctaga tttttaccca
     4021 taacggccat accgacaacg ccgatttgtt gttttgacat atgaactcct cctacatacg
     4081 ggatttctcc ctaatctctt acattatagc ttaacacggc tacattgcaa actaaagaac
     4141 aacatatttt ttatttaccg actacaaaaa aataaacctc aaggctccat ggttagcctt
     4201 gaggttttac attttctaat taaaatgtat taagcttctt ttgacattac gtccttacca
     4261 tcatagtaac cacaatttgc acatacgtga tggctttttt tcatttcacc acagtttgga
     4321 cattcgttta accctttgat tgtcaatttg tagtgtgtac gacgttttgc ttttttagct
     4381 tttgatgttc ttctagctgg tactgccatt ttgctacacc tccttgtaaa gtcttgtatc
     4441 taaattagac acaatatatt ccaatcttac ttgttatcgt cgtcttttga tttagaatca
     4501 aaaagttcag atagttttgc aagacgagga tcaattttat tctcggcttc ttccattttt
     4561 ctgcgctggt attcttcttc ggaaacaact tcccaatcat tgccttttgg aagttctgtt
     4621 gctgcttcct cttcttttgt caatacccgc attggaatcg ccaataagat gttgtcttca
     4681 atcgactctg ctaaattcaa taatggacct tcaattagca aaatctcatc tgcagacatt
     4741 tgttcatctc tactttggaa ttgttcttct gtcatgaata cttcgtctac agaaaaatcc
     4801 atgggcagtt caacaggttc tagtgaacgt gttgaaggaa ctgtcaaagt cgtctgcaca
     4861 gtgtaatgaa cgatatagcc tttcttatca actgagacca aaccttcgac ttccactggc
     4921 gcaacatcaa gtattaaatt gtctcgcttc atgagagcgg ctttcaagtc gagcgtctca
     4981 tgaaaagtta acggtgtctc ctggtatttt ctcaattcca ataaagacca tttcattttt
     5041 catcactcct aagcaacaaa agcaattata cagggcaaaa agcatgttgt caatgaaatt
     5101 ttcttgacac cttgcagttc ataaaaaaac agaaaaatct ttattagaaa gcctctgttt
     5161 gctatcatcc tcagaatcat ttacaataat cattattcca ttaaaggagc ggatgatctt
     5221 catgaacacg aaagatatcg aatactatgt ccaaatcgtc aaacaaaaaa attttacgaa
     5281 agtggctaat tatttccgtg tgagccaacc tactattaca tatgcattac aacgtcttga
     5341 aaaagagttt gacaccgtac ttattacaag agatcgatcg catcacgaat tgatcgtcac
     5401 gccttctgga aagcagctgt ttctccatgc acaaaccatt ctccaagaga tttctactgc
     5461 aagagctgaa atcactcagt tgcaaaagaa aaagcttcgt tttggcatgc ctcctattat
     5521 cggcaatgaa tactttccta aactgtcaag ttactttatc aagcatgaat tgatgaatca
     5581 aattgagatt accgatggag gctctcggga cttatacggg atgatccgac aagggaggat
     5641 cgatttagct gtattaggct caacacagcc aatcgtggat caatatttag aaacagagtt
     5701 acttttggac aaacgtttca tgatcgtcgt gccacctaca catcctcttt ccaagcagga
     5761 tactgtctct ttcaaagata tagcagatga acagtttgta ctattgaacg aacattatgt
     5821 ccaccccgca gcattcacta agctttctaa acaaacacat actcatccaa aagttgttta
     5881 tcaaagcaat gacttgacga ttttaaagag tatggtccga gaaaatgtgg gtatcggttt
     5941 tttggcagag attgctgtcc atcctgaaga tcatttagtt tgcctccctc tttcagatga
     6001 agttcagcct cgcttcttga tttcattggc aaaacgaaca cagcagatcg tcacgcctct
     6061 tcaagaacaa gtagtccaag ttatccatga attcgttaca gcacaaaaaa acagctcttc
     6121 gtaaaagaag agctgttttt attttttatt aagctaaatt agatagctgt tgtttcgcca
     6181 cggtaaacga tgccgcgacg tgaatcaacg gtaatcaatt caccttctgt aattgttgtt
     6241 gttgcgcctt tagcaccaac gattacagga atatcttgtg caattcctac aactgcagcg
     6301 tgagaagtca atccgccttc ttcaacgata acagcagctg ctttttcgat tgcaggcata
     6361 tattctttgt cagttgatgg tacaactaga accattccgt cttttgcttt ttttacagct
     6421 tcttcagcag atgaagcaat aactgcattt gcaacgacag atttttgtcc aacgccttga
     6481 gcttctaata atttagaacc aatcatttga attttcatca cgttagttgt tccgcgttca
     6541 ccaactggta caccagcagt gatcaagatt aaatcgcctt ctgatgcgaa acctaattca
     6601 acagcttttt tagctgctag ttcaaacatt tcatcagttg ttgatggttt ttcagcaact
     6661 gttgggtaaa cgccccagtt taacatcaaa ccacgttttg tgcgttcgtc aaaagtaaca
     6721 gccaagatat ctgcatctgg acgatatttt gaaatcattt ttgcagtgta tcctgattca
     6781 gttgcagcaa cgattgtttt cacgcctaga ttttttgcag cacgagctac agataaaccg
     6841 attgtttcag ttacatctgt tttatcgaat tcgttcaatt ggaatgtacc taattctgat
     6901 aatgcatttt cagcttcttg gtcgatacgt gccattgttg aaacagcttc aactgggtag
     6961 tccccgtttg cagattcacc agatagcatt gttgcatctg tgccatcaaa tacagcgtta
     7021 gcaacgtcag aagcttccgc acgtgttgga cgtgggtttt cttgcattga ttctaacatt
     7081 tgtgtagcag tgataactgg gataccagca gcattacatt ttttgataat gcgtttttga
     7141 accattggta ctagttcagc tgggatttca acacccatat ctccgcgggc aatcataaca
     7201 ccgtcagaaa ctttgatgat ttcgtcaatg ttgtcgatac cttcttgaga ttcgatttta
     7261 gggaagattt gaacatgagt catgtctttt tcttctaaga tttcgcggat atctagaacg
     7321 tcttgtgctt tacgtacaaa acttgcagcg ataaagtcga tatcgttgtc taaaccaaaa
     7381 cgaatatcgt cagcatcttt tggagtaata cctggcaaac taatagaaac gcctggtgca
     7441 tttacccctt tacgtgatcc tagcatacct gcatttttaa cttctacgac taattcacgg
     7501 tttgcttcgt ctttttcaat gatttgcata tcgatcaaac catcatcaaa taaaatgtgt
     7561 ccgccaacgt gaacatcgtc aaataatcct gggtaagtaa ccgcgatttt ttcttttgaa
     7621 cctacatgtt caggatccat agagatgcgt gtttgatctc ctacttcaaa tttgatgtat
     7681 cctgcacgac caaaatcagc ttctgttgtg ccttgaacag ttgtacggat ttcagctcct
     7741 tttgtatcta aaagaatacc gacatctttt cctgtctttt taactgcttc acggaccata
     7801 gtcatacgtg ataattgttc ttcatggtca ccatgagaaa aattaaagcg gaagacattt
     7861 gccccagatt caatcaattg agaaataatt tctactgagt tacttgctgg tcctaatgta
     7921 ctaacgattt tggttttttt catttaaaaa actctcccat tcaattttgt ttatttaaag
     7981 ctgtacacag cttaaatagc ttggtttctt tcactcttaa aatgaaagtg aattatttag
     8041 atcatatagt gataagtctg gtttatgttt gttgttttca agtgtatcaa tgatgtcaga
     8101 cgcaacgatt tcgttgtcat ggataccgac acataatcca ccttttcctt cttgaagcaa
     8161 ttcgacagcg taagcaccga atttacttgc caacacgcgg tcgcgtgcgc ttggtgctcc
     8221 accacggaca acgtgtccca agatagatac acgtgtatga tagtcaccaa attctgaaag
     8281 tttctcagcg aattcatttc cgcccattac accttcagcc aagatgatca agcaatgttt
     8341 cttcccgcga tcgcggcctt cttggatttt tttagcaact gaagccatat caaaatcatg
     8401 ttctgggatg atgatttcat ccgctccgcc agccacacct gaccataaag cgatgtctcc
     8461 tgcattgcgt cccattactt cgataacgaa tgtacgaaca tgagaagtcg ctgtgtcacg
     8521 aatacggtca attgattcta gtactgtatt gattgctgta tcaaaaccga ttgtgaaatc
     8581 agtcccagga atatcattat cgattgttcc aggaatacct actgctggga aaccgcgttt
     8641 tgtcaaagcc attgcgccat gataagagcc gtctcctccg ataaccacta gaccttcgat
     8701 tccgaatttt ttcaattgtt cgattccttt taattgacct tcttcagttg caaattctgg
     8761 ataacgtgca gaatacaaga atgtaccacc acgttggatc ttgtcaccta catctgccac
     8821 atcaagacga cgaatatctc cagcaactaa accggcaaaa ccataattga tcccgtatac
     8881 ttccagtcct tcgtaaatac ctttacgaac aacggcacga acagctgcat tcattccagg
     8941 tgcgtctccg ccactggtca aaattccgat gcgtttcata ataaacattc acctcattaa
     9001 aatactttat cctaagtatg acttaggttt acaaccattt ttgcgttaac cacgactaac
     9061 attctaacat tatttttctc acttgtcttg atttacggat aaaaaaatga aaaaaaatga
     9121 aagcaagtag tattttttac cgtaagttat ttgaaaacca cattatcttc gccaagcaga
     9181 taaataagtt gccgctttat actcttgctg tcatccaccc aataagcatc tgacagaact
     9241 acttttcgtt gcgttttttc gtgatacata actactggta tatatcctgg gtgtttctga
     9301 aaaatcactt gtaattgttt cattatctcg ggatcttccc gttcttctgt aatacgcaaa
     9361 tagcaagttt gtgaaggata ctgggcttct aactggttag gatcagcaaa gctttctcca
     9421 attagttgca gttcctgatt atatttgctg atttcgctcc gcccttgaac aaagatcact
     9481 cgatcaacgg caaacaaatt acgatattta cgataaagag ttggaaaaac tgtgacagaa
     9541 atttctccac tactgtcggt tccttcaaca aaggccatca gttctccttt ttttgtccga
     9601 atttctctaa cttccttgat catcactaat aaattgactg gttgattagg gacgatatct
     9661 gtaatcagct gaacttgttt agctaaacga agacgatcaa acccttctgc aggatgacct
     9721 gacaaataaa tacctaagta ctcctcttcc agcttcaagc gttcttctag tgtatagtca
     9781 gcgacttctt cttcttttaa tgccatgatt cccagcaagt caagacttcc accactgtaa
     9841 acgatatttt ggatttttcc ttcgagatca agcatcaatt gtttcctgtt aggagataat
     9901 tcatcaaaaa ctcctactgc tgtcagtgat tgcagtaact ctaatttcag ccaacgctta
     9961 tcgatccgta tgaggaattg atccactgac tggaacgggc cattttgttt tctttcgttt
    10021 aatatatctt ctacaaaatc acgccgaata ccttttatcg ctgtcagtcc gaatctgatt
    10081 tcttccttat tgaccaactc aaaactggaa ttactttgat taatggacgg cgaggaaagt
    10141 ttgagtttat ttttacgtgc ttctgcaata tattctttca attttgatgt gttatgccga
    10201 acagaattca tcaggctggt aaagaaagca cctggatgat gggcttttag atacgccatt
    10261 tggaatccca cgaatgaata agcaaatgca tgtgaccgat tgaatccata gtttgcaaat
    10321 ttttcgatat attcatatac ggtcgctgct gttttttccg aatatccctg ttgtaatgct
    10381 cctgatacaa aatgcgactt ttcttcatct aatacgtctt ttttcttttt actcactgcc
    10441 cgtcgcagaa tatctgactg ccctaatgta aatccagcca tagtcgaggc gatctgcatg
    10501 atttgttcct gatagacgat cactccatat gtgtttttca aaatcgggat cagtgaatca
    10561 tctgggtagc gaatggcttc ttttcctttc ttcctggcaa tgaaggtatc tatattttgc
    10621 atagggcctg gtcgatacaa tgcattgact gcggcaatat cttcgatgtt ttctggatct
    10681 aatcttcgta aaacgttccg gataccagca gattcaaatt gaaatactcc tgaggtttcg
    10741 cctcgttgaa acaagagcag cgtttttgca tcatttagcg ggatctcttt taatcggatc
    10801 tcataccctt ctactcgctt aacagctttc aaagtaaaat caataatcga taagttgcgt
    10861 agtcctaaaa agtccatttt caaaagaccg attgcttcta cctcattcat cgtatactgg
    10921 gtcagaaaag cttcatcaga accgcgttgg agcggaacta gttcaagtag attcttatcg
    10981 ctgataacta cacctgctgc gtgagtcgaa acatgtcttg gcaatccttc taaaatcact
    11041 gcagttttga ataaaagttg gtttttttcg cttgcctgaa ctaattctac taattgatgc
    11101 gaagttttat aagcttgggt caatgtgatt ttcaattgat tgggtattgc ttttgaccag
    11161 cggtttgctt cactttgcga taaaccaaac actctagctg catctctaag aaccattttt
    11221 gctgccattg tgccaaatgt tgcgatttgt gccatatgat attgtccata cttttctcga
    11281 acataattca atacttcttc acgtcgattg tcaggaatat ccaaatcgat atctggcatc
    11341 gtaaagcgtt cggggtttaa aaagcgttcg aacagcaaat catattctaa tggatccaca
    11401 tctgtgattt ctaacacata ggctactaat gaacccgcag cagaaccacg acctgcaccg
    11461 gtaacgattt tgtttctatg agcaaaagcc attacatccc atacgatcaa gaaataatca
    11521 tcgaatccca tttgatggat gacagataac tcgtagtcca aacgttcttg gtattccttt
    11581 gtatagtcag tgattcggcg ttccacttgc gaatagcata actctcttaa atattctcca
    11641 gcttgttttc cattaggaac tggataatgc ggaagtaatt tctgatggaa ctggaattca
    11701 aatgaaatcg attcagctaa acgctgggcg cctattacag cttgggaaag acccgctttt
    11761 tcgaatcgtt tgatcatttc ttgttctgaa agaagataga catttgctgt tagtggtaaa
    11821 gatgcagata aattttctaa aggtatttgg cttccctctt tgatatgttt catcacttca
    11881 agtgcaaaaa gttcttcttg ttgtaaacta gcgatgggat gtaatgcaca aagcgaataa
    11941 ttttctttta tcacatactg ataccattca ggcaattgat caggcgtgtt ttctgtcaag
    12001 ccgatataaa aatttctagt aaaaacattt tcaagtaaaa actgccaatg accgaaaaag
    12061 ttatcctctt tattgaaagc ctcagaaaca gaatgatttg ccggaacaac aaacgctaga
    12121 tggtcggttt ctgtcatttc tgcaagttcc aatttttctt ggatcatttt tcggctggaa
    12181 agtttcatca gctgctgata acctatttca tcttttgcgt acgcaagcaa gccataattt
    12241 ttttcttcat aagtataact aagatgtaat ccaatgattg gctgtactcc ttcttctaca
    12301 caaatacgat agaattcagg tgcaccgtat aagacatctt tatccgttat ggctaatgaa
    12361 gtgtagcctt gtttttttgc ttcttgtaca tattctcgaa tacgtatcgt actttgtaaa
    12421 agagaataag aagtaattgt ataaagttgt gggaaaaaca tcttgcattc cgcctttttt
    12481 ttcgttaaaa ttcactagcg ttaaaaggtt tgatcctttt actttcatta agatagcagg
    12541 cgctgaagtt gattattcca cttcatttgc ttgcttgtaa tctgtaggaa tgagttcttt
    12601 cgcaagaact caaattttat tgagatttgg tctcgtttct ttacattcaa agaaaatctg
    12661 gtaaaattaa gatagaaaag aggtgttctt tatgcgatat atcgttacat tattctgggc
    12721 tgtcgttcta ggtcaagtag tcggttatat tggtgctgca ttaacaagcg gaacatatga
    12781 ttttactttg acaacaatca tttcattcat tgcgggtgtc attatccttt tgattggtgc
    12841 agttgcgcca agaaaagaaa cctctgccca ttcgtaaaaa aacagccaag agcttgagat
    12901 ttctcaagct cttttttatt ttctactcct caccaccatt ataatctgct gcgaagaact
    12961 ttacgaaagt tgaataaaga aagaagaata tttgacagaa caaacaaact tgtcacaata
    13021 aaaacagcac tgtaattata agcatgcgcg actgtcgatc ctagcatggg acctaaaact
    13081 tgtccaaagt tgctaaacat ctgattgtag ctatagattc ggctgactcc ttcttgaggc
    13141 gtaatcttac tgatcagtgt attgacagaa ggcatcaaag cgccagttga aaagcctaaa
    13201 ataaagcgaa gtatacctaa ttggagcggt gtagtgacga atgccatagg taaataacag
    13261 caaaatgata aaatgagtcc tccaattaga actttttgat tcccaatctt atcccctagt
    13321 tttcctagtt tcggtgaaga aaagacagcg gaaacaccag ctatcgaaac aatcaagccg
    13381 ctgacaaaaa ggatattttc tgtacttccg ctcaattcac gaatatataa ggtcaaaata
    13441 gggctgatcg tagtgattcc tatctgtaaa atcaaggtcg taatgaagag accgatcaaa
    13501 atagagagat gatccatttt tgaaaaaatt tctttggtgc ttatcagatc ttttttctcg
    13561 attggatgga aatcttcttt gactaagaaa atggtcaata ccgttgtgat catcaacaaa
    13621 gcccctgtga tcaagaatac attttccata ccaaaccact gagccagtgc accgcccatt
    13681 gaaggtccga tcagatttcc agcaatcgct ccggtagaca atgttcctaa cgcccatccg
    13741 ctcttttctc taggtgcctg tgaagcaatc atcgctgtgg catttggaat atatccagac
    13801 aaaatcccat tccaaaaccg catgatcaaa agccaatacg cgtttggtac aaaagccaaa
    13861 gatcccattg ttaatgtcat ccctgctgct gcacggatca tcatgagttt tcggcctttt
    13921 cgatcagcta gatttcccca gattggtgca accacggcag atgcaaaagc agtcacagag
    13981 attgctagtc ccgaaaacaa ttcgatttga tcttttggtg ttcctagttg ctcaacataa
    14041 actggtatga atggcataac taaactaata cttgcaccag tgaaaaaaca gccaatccac
    14101 gcaatcacta aattttttct ccaattaatc tccatctatc atccctcttt cgtctctttt
    14161 atcataccta ttttcgaata aacaagcaac gatctcgcca gtaaaaagag aaaaatcccg
    14221 caaaggtcat tttatatgta acgttctttt tatgtaaaac aaaaagagat cagaacaaat
    14281 actgttccaa tcccttctct ttagttaaat tgttgctaga atgatgaagt tgataatgaa
    14341 taaaatatca acgatccaca aagcaacaga aacttgattg tattttcctt tgattacttt
    14401 cacaattgta tagaaaataa atcctgctgc aatcccatag gaaatgctat agcacaatcc
    14461 cataaagatc gatgcaaaga aggccggtaa agcttcttcc atatctagcc atttgatgtc
    14521 tgcaaaagaa gccatcatca ttactccaac taaaatcaat gctggcgcag ttgcttgatt
    14581 tggtacaatg gcgatcagtg gagagaataa actacttaat gcgaatagca ctgccactac
    14641 tactgaagtc aaaccagtac gtccaccggc cccgatccca gctgcagatt cgacataagt
    14701 tgttgtattc gatgtaccaa agatagcccc cacagaagta gcgatcgcat cggcaaatag
    14761 tgctttatcc atttttgttt tgaacccacg accatcttcc aacgcgatct cgtcttcttt
    14821 tgagaaaata cctgtgcggc gtcctgtacc gataaatgtt ccgatcgtat cgaaagtatc
    14881 agataaactg aatgcgatga tcgtcatcaa tacttgagga atcttggaag aatcactgaa
    14941 taaagattgc atgccttcag aaccaaatgc tgcaccgaaa gttgttccta attctttaaa
    15001 tgaattacct aacgaattcg tttgccaatc aatggaactt aaatcaacaa cacccataaa
    15061 aataccaagg actgtggtcg ctacgatacc gataagaata gccccacgga cattttttac
    15121 aactaaaatg gctgtcagta ccaaaccaat cacagccaat aagataggag cgttgtcaaa
    15181 gttggctaac gcagggacga ttccaccatt tatcgtaaca tttgtcgctt ttccgccttc
    15241 aacgacagag ctcgaaatag ctgactggtc tgctgaaaaa gataagaatc ctgcattttt
    15301 gattccgaca tacgcaacga aaataccgat cccgccacca atcgcatgtt gaagactttc
    15361 gggaatcgcc cgaataatca tttttcgaat atttgtgaca gtaataaaaa tattgattaa
    15421 tccgcaaata aacaccattg ccaaggcttg ttgccaagaa taaccaagcc caaaaacgac
    15481 cgtaaaagtg aaaaatgcgt tcaatcccat accaggtgcc tgagcatatg gtacatttgc
    15541 gaacaatccc attaccagcg taccaataat cgatgcaatg attgtcgcta gaaatactgc
    15601 ttggaagggc atacctgatg ctgacaaaat cgtcggattt acgaataaga tataactcat
    15661 ggcaaagaat gtcgttactc ccgccattat ttcagttgat acatttgtat tgtttttctt
    15721 taaaccgaaa aacttttcca taaaaagttc cactccctga atttttttct gatggaaaac
    15781 tataataata aaaaataagt aaaatagggc aaatccatcg aacaatttca aattatatag
    15841 acgtttcatt cgcttttcaa cgttttttta acaaaaaaag cgaaaataat acttaacgtt
    15901 cgtgtttagc ttatgtttag ctatttatat agccataact gtagtttttg ttaggaatag
    15961 ctgattgctc ttgttaatta cctaaaatat gctagactaa aacaaatgct agttgaggaa
    16021 ggaaggaccc ttattgaata aaaaattaat tgccatcgat ctggacggaa ctaccctcaa
    16081 tcaagattcg atgattacat ctaaaacaaa agagactcta aaaaaagcaa tcaatgccgg
    16141 tcatcatgtc agtattgcta caggacgccc attccgaatg agccatcagt tttaccaaca
    16201 attagaatta acaacaccga tggttaattt taacggcgca ttagtacaca tccctaatca
    16261 gcactgggat ggtgaaagag aaacgttgat caatcgtgaa atcgcttttg aaatcttatc
    16321 gcaaaaaaag cagctaaatt tagattttat cgctgctgaa aatcgtgata ctttttttat
    16381 tgacagtttt gatttctttg atgaaaaaat ttttgcttct tctcgtccag gagagaagaa
    16441 tttgctttct cctaaaaatt tgacgacaaa tcctacatct ttgcttgtcc gaacagacaa
    16501 acgttttgca ggagctgtat ctgctgaatt aacacgtcaa tttggcagtt atgtcgatgt
    16561 tcggacatgg ggtggaccta ctgccatctt agagattgtt tcaaaaggaa tccaaaaagc
    16621 aaaaggcgtg caagagattg ctaactacct gtctatcgat caaaaagacg tcattgcctt
    16681 tggtgacgag cataatgatt tggaattgtt ggattatgca ggttggggag tagctatggc
    16741 taacggtact gaccaactca aaggcattgc aaatgatgtc actcctttga gcaatcagga
    16801 agacggttta gctgtttatc tggaaaaact acttaaacta taatagagaa ttagattcca
    16861 caaaaaagag gttgtgacaa t
//
LOCUS       DACDRN010000051        16672 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDRN010000051 DACDRN010000000
VERSION     DACDRN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.051"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1129
                     /locus_tag="IWJ80_001974"
     CDS             <1..1129
                     /locus_tag="IWJ80_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HAP9461685.1"
                     /translation="FGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHI
                     KANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISK
                     ADPESGYYVKGEREKQFAYSAHTSCDDNGFILSTIITPGNIHDSQVAFQLVKQSKRLF
                     PEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYIC
                     PQDQMLAFSTVTREGYREYKSNPKECVNCPLLNQCTKSKNHQRVITRHVWGDLMDEVE
                     HLRLTDLNKSIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKK
                     LATWLWKGKEPLFFCSKIRNEVDKKLFQARVTSLEQLLSTV"
     gene            complement(1158..3239)
                     /gene="cadA"
                     /locus_tag="IWJ80_001975"
     CDS             complement(1158..3239)
                     /gene="cadA"
                     /locus_tag="IWJ80_001975"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9461686.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(3763..4122)
                     /locus_tag="IWJ80_001976"
     CDS             complement(3763..4122)
                     /locus_tag="IWJ80_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP9461687.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(4152..4493)
                     /locus_tag="IWJ80_001977"
     CDS             complement(4152..4493)
                     /locus_tag="IWJ80_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461688.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(4490..5164)
                     /locus_tag="IWJ80_001978"
     CDS             complement(4490..5164)
                     /locus_tag="IWJ80_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9461689.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(6341..7639)
                     /gene="asnS"
                     /locus_tag="IWJ80_001979"
     CDS             complement(6341..7639)
                     /gene="asnS"
                     /locus_tag="IWJ80_001979"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP9461690.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(7679..8869)
                     /locus_tag="IWJ80_001980"
     CDS             complement(7679..8869)
                     /locus_tag="IWJ80_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9461691.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFLKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(8890..9417)
                     /locus_tag="IWJ80_001981"
     CDS             complement(8890..9417)
                     /locus_tag="IWJ80_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9461692.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(9464..12253)
                     /locus_tag="IWJ80_001982"
     CDS             complement(9464..12253)
                     /locus_tag="IWJ80_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9461693.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFIYVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMTTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLISTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(12400..12600)
                     /locus_tag="IWJ80_001983"
     CDS             complement(12400..12600)
                     /locus_tag="IWJ80_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9461694.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(13052..13372)
                     /locus_tag="IWJ80_001984"
     CDS             complement(13052..13372)
                     /locus_tag="IWJ80_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461695.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(13756..14874)
                     /locus_tag="IWJ80_001985"
     CDS             complement(13756..14874)
                     /locus_tag="IWJ80_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461696.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            15345..16652
                     /locus_tag="IWJ80_001986"
     CDS             15345..16652
                     /locus_tag="IWJ80_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP9461697.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
BASE COUNT         4799 a         3533 c         2978 g         5362 t
ORIGIN      
        1 ttttggaaag aattatgttc gccgatttag agaaacgacg gtgtttgagg atatttttgc
       61 ctatatttta gaacaagcag ttaaagcagg ttttgtaaca gaggataatt tgtatctgga
      121 ttctacccac atcaaagcta acgccaataa acataagttt acgaaagaaa tgacccatga
      181 tgaagcgaaa gcctatcagg atgaattaga agatgagatc aatcgtcaac gaatagaggc
      241 tggaaaacgc ccttttactt tggacttaga aaaggaagtg aagctgaaag aaagaaaaat
      301 aagtaaagct gatccggaaa gcggctacta cgtcaaaggc gaacgagaaa aacaatttgc
      361 ttattctgcc catacttcat gcgacgacaa tggatttatt cttagcacaa tcatcactcc
      421 tggaaatatt catgacagtc aagtcgcctt tcagctcgtc aaacaatcga agagactttt
      481 tcctgaaatc aattgtgttg tcgctgatgc tgggtataag acacctaagt ttgttcactt
      541 tttgactcat ttgaaccttc gaccttgttt gccttattca aggcccaaag ggaaaaaggg
      601 gctattatca aaaaatgagt ttctctatga cgagtatttt gattgttata tttgtccgca
      661 agatcaaatg ctcgcttttt caacggttac tcgagaaggg taccgagaat acaaatccaa
      721 tccaaaagaa tgtgtgaact gtcctctgct aaaccaatgt accaaaagta aaaatcatca
      781 acgagtcatc acgagacatg tttggggaga tctgatggat gaagtggaac atcttcgcct
      841 aacagatctg aataaatcaa tttacaaaaa acggaaacaa acgattgaac gaatatttgc
      901 ggatgcaaaa gaaaagcatg ggatgcgttg gaccaagtat cgaggcttgg aaaaagtcgc
      961 gacgcatacg atgctagtgt ttgctgcaat gaatttaaag aaattagcca cctggctatg
     1021 gaaaggcaag gagcctttat ttttttgttc aaaaattcga aacgaagtag ataaaaagct
     1081 gttccaagct cgagttacga gtttggaaca gcttttgtct acagtctgag aggacgttca
     1141 ttcgtcctct tcattgatca acgtttattt tttacatgca agcttcgcat ggcattcaaa
     1201 acagctaata ctgtcacccc aacatctgca aatactgcat ctcccattga tgcaaaacca
     1261 aaagcaccta aggcaagtac caaaattttt atcccaatag caaagacaat gttctgtttg
     1321 acgattttca atgttttctt tgctaaatgg attgcttctg caatcttcga tggttgatca
     1381 ttcatgatga ctacatctgc tgcttcaatt gcagcatcgc ttcccaatcc acccattgcg
     1441 attcctacat ctgctcttgc tagcaccggt gcatcgttga ttccatctcc gacaaatgcg
     1501 gtctttttat tgtttctttg taagatttcc tctaaccgct gcactttatc ttctggaagc
     1561 aattcgctat atatcttgtc cacgcctact tgcttcccga tatgatcagc aatttttttc
     1621 gaatcgcccg tcaacatgac tgtgttcttc acaccagcct tttttaattg aaccaatgcg
     1681 tctacggcat cttctttcaa ggtatctgca ataacaagat aaccactgta actttgatcg
     1741 atcgctagat aaagcaatgt accgatttct tgacttgaag gaaaagagat ttcaaatctt
     1801 tccatcaatt tgtgattacc tgctaatact tgtttgccat caacttcagc taagatccct
     1861 tcacctgcaa attcttggat attagctgtt ggtaaaagcg gacctgcgta gccttctaaa
     1921 acagattgag caatcggatg tgtagaaaac tgttctgcac tcgcaacata ttgcatgaat
     1981 atctttggat cgatttttgt atcgatcgtc tggatcgaaa agtcaccttt cgttaatgtg
     2041 ccggttttat cgaataccac tgtctcagtt tgtgcaagca actccaagta atttgaacct
     2101 tttactaata caccgatttt acttgctcct ccgataccgc caaagaaact aaggggtacg
     2161 gagatcacta atgcacatgg acatgagatc actaaaaatg tcaatgctcg atagatccat
     2221 tcagaaaacg ccgctccaga aacaacaagt ggaggtaaaa cagctaataa aacggccaat
     2281 cctacaacga ctggtgtgta atagcgcgcg aatttagtaa tgaaattttc agctggtgct
     2341 tttttactgc tggcattttc tactaattct aataatttgc tgatagtaga attttcataa
     2401 gatttttcta cttgaactaa aaatggttta tttttattga taaatccact taatactgtt
     2461 tcaccaacat atacagattt agggactgat tctcctgtaa gtgctgacgt atcgactaat
     2521 gattctcctt cgatgatcgt tccatctaac ggtacacgtt caccaggctt cacaagtaca
     2581 aactgaccga tctgcacgtc ttcagggttc acggtcatca atccttccgt cgtctgcacg
     2641 tgagctgttt ctggacggat cgccatcaat tcgcgaatcg attttcttga atgactgact
     2701 gccaatcctt ggaaatactc gccgatttga taaaatagca tcactgctac ggcttctgga
     2761 tattcaccga tcaacatagc gccaatcgta gcaacagaca taagaaaatt ctcatcaaag
     2821 atttttcctt tgacgatatt catcactgcc ttcttcacta catcatatcc tatcagcaga
     2881 taaatcaata aataccctat caagcgaatt ggttcattaa aatcaaataa tagcaataaa
     2941 gcgatacctg caagggaaaa taaaatccgg aacagcgtat ttctctcttt ttcatttcca
     3001 tgttcagata ctttttcctt atctatttct gttactttga catctggttc taattccttc
     3061 actacttttt ttatttcttg ttcaatcgta gccaaatgat cttcctctgc gtcaatcgtt
     3121 agttttccag aagtgaagtt taccgttgct tccttgactc cattgatctt ctgaactcct
     3181 ttttcgattt tcattgcaca gttcgcacaa tctaaacctt ctaatctgta acttttcatt
     3241 ttaatgacct cctcgcctca acatatgaat atctcttcat aactaattat aaatgaacgg
     3301 ttattcatat gtcaaggtat ccgaaagaaa aaatgaaaaa catctgcatc ggaaaatttc
     3361 ttccatacag atgttttggc ttattctttc tttttctttt cgatcattgc cactgctctt
     3421 atccaacccg cacttgcctt cttgatcttg acaggatagc aactaagttt gaagcctgtt
     3481 gctggcaatt tgtctaaatt cattaatttt tcgataggac aatatctttt ttcaatccct
     3541 gcaaaatgtc cttcccagat attcgaagct ccactacgct ttcaaaaata tagaaagagt
     3601 tctttaatga aaattatttc aactttccat aaacagtaac cccatataaa taaaagacgt
     3661 aaaagtcaca tggaagaact tatacaggag aacgcataga gtcaggtgaa atacatgtat
     3721 cccacctgac tctatgcttt atctaacctt tattattctc ttttatacaa tttctgcacc
     3781 gaatggcatc aacgccaatc gagcaagttt gaaagattgc ttggcaaaag gtatccctat
     3841 gatcgtgata catagaagga tgccgctgac gacatgtgcg agagcaattg gtaggcctga
     3901 aacgatcagc cagatgatat taacaatcaa tgacatgcca gactggtgat aaattacttc
     3961 tttgccaaaa ggcgcaaaag aaagggaggc gatcttaaaa cattgcatac cgattgggat
     4021 acctatgatc gtgatacacc aaagtatccc cgctgctaac cagctaagac caccgattag
     4081 tccgccgaat ataaaccaga tgacatttcc taaacaactc attgtatttc tccttctatt
     4141 actgtttttg attaaaactg ggcaaaaaat gattttattc ttcttttttt agcttccagt
     4201 atcaaatatt ctgcaaattt tttttggatt tcttccgtat tttcttttgt tactaatgtg
     4261 tgttggatta gttttcctga tggtcgctga tccaatcgtt cttctgactg ttgcacgtcc
     4321 gcttgcgcaa atccgataat ccgtttttct tttgttacag caacgagcaa tagtttattc
     4381 ttatcattta ggtcagctgc cgtttttgct tttacgaatg attctggatg atcagtaatc
     4441 agctgattga tttgaggtag atcttctttt actgcccacc gtatttccat catttctcac
     4501 ttttctttcg ctcattttta tctccactaa aaaaatcgac tgcggcaatt gcatctatcc
     4561 agctttgtgc aatgatctca tgtccgccaa tcgttgggtg gaccccatct tctcttgtat
     4621 atttcgaaaa accataaaca atccctaaag cattgaatat accatctaaa ggaaccagca
     4681 atgcgccata ttccttagca agtttacgaa caatttggat tttagggtct aagtctttac
     4741 gccagccatg acggtcgtcg gttataggaa gtacaaacgg ctccatcaag atgatgttgc
     4801 agattcccac tgtttgaagt tcatttagta attttcgata aacggattca aattgctgag
     4861 cagatttctc agttccgaat atagcctgat cgctagtatt atgccaggta tcattgattc
     4921 cgatcaaaat cgatacatag tcaggttgta aagctaaaca atcttcttgc cagcgtactt
     4981 gcagatctgc aattttgtct cctcctatcc cacggttcat gcattcaaat tccatcgttg
     5041 gatattggga gattagtttt ccggcaatca aaagtggata acctttacct agatcagttg
     5101 cttctgctcg gtctcttcct gcgtctgtaa tgctatcacc gataaataat atacgttttt
     5161 tcatttgtca aaaactcctt tttgaataaa acgatgattt tcttccttat attatcccat
     5221 aagccctatc aagacgaaag agaaaatata tgtataaaaa atgacaccag cctagtccct
     5281 tcaaacaatt gaatgaacgc tcatcaagtg tctagttttt tatgctattt ttttcttaac
     5341 gtgccggatt ccccttgatt ccttcgttgt ttggtccaga gagatcgatc acacctaagc
     5401 ttccgtcaat cagaccactg atgacgccat cagaacatat atttataggc tcatctaaga
     5461 cttctagtaa atgtcccttt actaatatct cttctagatt gcttgggata gattggtcat
     5521 aactttatct ggtccgatga tttcaagggt gaattgaact tacatatgga ttattgcatg
     5581 ctgaaaatac ggacctactc catcttcgaa tgttactatt agtttgtttt caggtgctag
     5641 atgctgcttt atttcaacag cagaagcatc atcaaatcag atttccattt gaattacctt
     5701 ctttgtaaat gtttgattga ttgcgatttc gttatagaac cttgtgaatc aaatagcaat
     5761 ttcttttgct ttcttctctt gtctgctgga aacttccttt atcccgtatt ttaaaaaaag
     5821 aactgcgaga gttcgcagtt cttttttaga ataaacggtg ctctccggcc aactcctcgg
     5881 tcacaagtca caatagtcat caaacatgaa aaactgtttt ttgaaattac tccttgtgac
     5941 tcggagctga acggcaggcg cacaacctct tgatataaac ggtgttcaat cagctgaccc
     6001 tcgatattag ctaaaaaaac ggtaaaatat gagaagctat ttttccgttt tgtttctcta
     6061 atactcaggt caaggcactg atttcacaac ctcttcctta aacggtgttc aaaagccagt
     6121 ttctcggtat taagccaaat ttatgcaaaa atgtggaaag ccatttccac aaaatttttc
     6181 ttaatactcg aaactaaaca gctttttcac aacctctcca ctaaacggta ttcaaaagct
     6241 tacatctgcg gtaataagtc ccgccaattc aaaaacatga acagctgttt ttgcttggct
     6301 gttctaatta cttgatgcag aacagctttt tcatcacctc ttatggataa attcggttca
     6361 acaaacgtgg gaatggaata gcttcacgca catggtcgat accagcaatc caagtgactg
     6421 ttcgttcaag gcctagacca aaaccagagt gcggaacaga accgtattta cgtagatcaa
     6481 gataccatga atattctttt tcatccaaac cgtgattacg gatttgttct agcaagaaat
     6541 catagtccgt tgcccgttcg cttccgccaa taatttcacc gtaaccttct ggtgcgatca
     6601 tatctgcaca aattaccacg tcttctcttg ttggatgtgg tttcatgtag aatggcttga
     6661 tagcttttgg atagtttagg ataaagacag gctgttcgaa tgaattcgca atgaacgttt
     6721 catgaggtga accaaagtct tcgccccatt cgatgtcatc aaatccattt ttcttcagta
     6781 attcgatcgc atcatcataa gtaatacgag ggaatggcaa ttttgtataa gtttttaaga
     6841 cttctttgtc tcgtcctagt acatctaatg cgtattcgca atgatctaaa acattttgta
     6901 ctaagaatgc tacatattgt tcctgtactt ctaaactttc ttcttgatga gtgaaagcca
     6961 tttctggttc gatcatccag aattcaatca aatgacgtcg tgtttttgat ttttcagcac
     7021 ggaaagtcgg accgaatgaa aagacttttc cgaaagccat agcagcagct tccatgtata
     7081 attggccact ttgagataaa taagcttttt gatcaaagta atttgtttcg aatagatccg
     7141 tagtgccttc tggtgcggaa ccagtcaaga tcggcggatc gattttgaca aaatgatgtt
     7201 gattaaagaa ttcatacgtt gcacgaatga tttcatttct gatctgcata atcgcatgtt
     7261 gtcttgaaga acgtagccat aaatgacgat gatccatcaa gaagtctgtt ccgtgttctt
     7321 ttggtgtgat cggataatcg tggctctcgc ttaccacttc cacatttgta attccgattt
     7381 cataaccaaa ttttgaacgg ctgtcttcac ggatttctcc agttactaac acggatgttt
     7441 cttggtgcag atttttagct gtttggaaaa cttcttccgg cacttcgctt tttacaacta
     7501 caccttggaa aaaggctgtt ccgtctctta attgtaaaaa tgcgattttt ccgcttgatc
     7561 gcttatttgc gacccaagcg ccgattgtta ctgtttctcc cacatggttt ttcgaatcga
     7621 tgatttgaat ttgttccacg tatgactctc cttttatatt ccttgaattt cttcaggatt
     7681 aattttgact tttactctca ataaattgct tgattctgcg taccgcttct tctaacgtag
     7741 cgcgatcagt ggcataactc atgcggacat tttctggtgc cccaaagcct tcgccagtca
     7801 caagcgctac ccctgtttct tctaaaagat cttctaccca ttgtgtgaca ttttcatatt
     7861 tacacatgcg tagcgtttct tttacattag gaaaaagata aaaagctcct tgtggctttt
     7921 caagagaaac acccggcagt tctgccacaa gcggatataa cgtattcagg cgttcttcaa
     7981 aggcgttccg catttcttcc actgtatctt gctcgccttt caatgcttca acagctgcat
     8041 attgactgac tgctgtcgga ttgctagttg attgggaagc aacagcgatc attccattga
     8101 tgatcacagg atttcccacc gcataaccaa tgcgccagcc tgtcatcgca tatgttttag
     8161 acacgccgtt gatgatgatg gtctgatttt tgattgcttc tgaaatcgtt gcaattggag
     8221 taaactcatt gccattataa actaaacggc cataaatatc atcagcaact atcaaaatat
     8281 cattttttac tgcccattca ccgattgcct gcagttcatt cttgctataa atcattccag
     8341 tcggattcga tggagaattg atgattacgg cttttgtttt ttctgaacgt gcttgttcta
     8401 gctgctcaac agtaactttc cagctattgg attcttctcc tttcaaaaag acaggaagtc
     8461 cttctgccag ttttacttgc tcaccgtaac tgacccagta aggcacagga atgatgactt
     8521 catcttgcgg gtcaaggatc gtttggaaaa gggtatacaa agcaaatttc gctccgtctg
     8581 ttacgactgt ttgtgaaggt tcatagacaa gtccatagtt tttcttcaga taatcaacga
     8641 ttgcctgtcg aagctctggg attcctgctg ttggtgtata atagctagct ttcccattgc
     8701 ggattgcttc gattgctgct tcttggatgt tttctggtgt cgcaaaatcc ggttctccca
     8761 cagtcaaact cagcacatct ctgcctttgg cttttaatgc cttggccttt gccgcagctg
     8821 ccaaagtgac agacggttct aatcgttttg ttctttttga taactccatc ccattcattc
     8881 ctttaccttt taaattccct tgattgtctt gacttcatct ccagattcaa agccaatcag
     8941 gtaataattc agtttcccat ttgtttcttt tcctactact tcccacacag gctgatcatc
     9001 atacatccct aaagcagctt tttcgatttg gatttcagga tgggccgcag caattgtctg
     9061 ttttgcttca ttttcagtca gtccctttgt ttgatccaat acttttactt tttcaccaga
     9121 tttaggaatg ataacggcga tttcttttcc tttatcattt gtacctgtca gtgaaaagta
     9181 tgtcttcttt cgagtaaacc aataaaaatg atcggcttcc tcaagatttg catatttctt
     9241 tgcgatttct actgcttctt tcttcgcctg caccgccgga cgactggaac gcaagtaaaa
     9301 aatcgagctg atcacgatga ccgctaataa aaatacgatc aagccaagta acacgcgatt
     9361 aatcgttttt tcctttgtct gttttttctt caagggtcgt cgctccttta tcttcattcc
     9421 ttttcattat agcagatttt aattatgaaa aaagacagct cctctaactt ccttcttcat
     9481 ttttctttaa gaattcatgg atatcctgat agatttcctc catagctagc tctttgatcg
     9541 gtactttttt cggtaatgca tggatgatcc gtgcaccata ttttgcagta actagtcttc
     9601 gatccaaaat aagcaatacg ccacgatctg tctccgtact tatcaagcgt cctaatgctt
     9661 ggcgtaactt caaagctgct ttaggtacgg attcctggta aaaaggattg atcccctctg
     9721 attctaaata agcgttccta gctttgacca tcgggcgaag cggattatcg aagggtaaac
     9781 gtgtgactac aacgatctgc aaagcatctc caggcaaatc tacaccttcc caaaaactgt
     9841 cagcgccaaa caatacgctt ttttctgaca aaatgaagcg tttcaaaagc ttctcccggc
     9901 ttccgccaaa cccttgagct agtatttctc gtcctttttc tagcatagac agatgcattt
     9961 tctgatagac cttctgcaaa acatcatgag aagtgaataa cacaagtatt ggccgatctt
    10021 cttgttcgat cagacctgta agtgctgtct ctaaataatc gatatatgct tgattgctga
    10081 cttgatggac ggcaggtgcc tcttttggca catagatcct tgcctgtttc tcataatcat
    10141 aaggagaggc aatcacttta agaggtgctg cttcaatgcc aagccgctta gagagatagt
    10201 gtcgattagg gccgatcttc agcgttcctc caatataaag aatacgatca tatcgaggat
    10261 accaaacagt cgacggcaac atagccgctt gaaaatcatg gagatgaaaa atcatctgct
    10321 gcgctgtgtt cccaggaacg aaccaatgga tcaaatgatc tcgccagtct tccagccatt
    10381 gatccatcag caagtactgc ttgtctaatt gatcaaataa gctatataaa cttgctaaat
    10441 cgatccgcat ctggtttgtc cagctttctt ggataatctc aaaagtcaga cgcatttgtg
    10501 actgtagctg ctggatctcc tgataaaaaa gctgcaggtg atcaatggct gtcccagatt
    10561 ccagtggcag ctgatccaac atctcttttg tcgtcatgac ttcttctgga tactgtccgt
    10621 taggttgctt gccgctcact aaactcatta atccttctgc catacaaatc tgtgcttcgc
    10681 atagcgcaga aagttcttct cgataaatcg ataaccagcg taaggcttca tgttctttcg
    10741 gtaaatagct ttgtattcgg tcaaacagct gatttggttc ttctaattgc tggatttgtt
    10801 tatggaaata gttaatactt aatcgtttgt catttacttt ttcagcgatt tccggcaaat
    10861 gatgtacttc atcgatcaac aaaaatggac tagctggaag caatggttct tttcgctgtg
    10921 tttcttgtat taaaaacgca tgattgacaa tcaaaaaatt actttgcctg attttctttt
    10981 gcaagaaacg aacaaagtct tgttcgtaaa atgactgatt tcttgctaaa aatccagtgc
    11041 ctctgtgcgc gatatctgca aagaatgaat ggttcaaatt tgttaaatgt aattcatcca
    11101 aatcaccggt cttcgtttgc gtcagccaaa ctaagaccgc catttgatac aaagcatatt
    11161 gtttttgttc tggcggttcc acaagtgtgc ctttaaaacg ctgtaaatca atatagtggc
    11221 gatagctttt gataattaca ggttgtatgg gctgatctag caattgattc agcaatggaa
    11281 tgtctttttc tatgatctgc tgctgcaaaa ctagcgaaac ggtactgatg acagcgggtt
    11341 tttcaggtgt agccaaataa ctcaatggca gcagataacc gattgtttta cccattcctg
    11401 tagctgcttc gatcatcaaa tcttttgatt catcttttgt gaaatggtcg tagaccgcat
    11461 tcatcagtct gttctgttct tttcgataca tcagtgtttt accaaacatt ttttcttttg
    11521 cttgtttttt tcttggatat gtccgctcgc cataatggac gtatatgaat agttccactt
    11581 cttttttctg caaagctagc ccatcgacga tttctaatga gggatcaagt ggctctggtt
    11641 tttctttcat ttcttcaatc acatgataaa tgaaacgact cgtatccata cctgtgtgtc
    11701 tacttaatcg cgcaatctgc tctaacgtga tgattggcaa ttttctcatc cgttcttcga
    11761 tcagcaaaag aagttgaccc gtcacttcgg catcactatc tgcctgatga ggattctcat
    11821 gaataaatcc aaggctttct gacaaatcag ctaagcgaaa gcttggttcc gtcggcaaga
    11881 aaatttgtgc aagttctact gtatcgatgc ctggtatctt caaactagga agtccgcatc
    11941 gcatgagttc atggttcaaa aaattataat caaaataaat attatgcgcg acaaatatgg
    12001 tgtctgccaa aagattgtaa atcgtttggg cgacatcttc gaaataaggc gctttttgga
    12061 cgcgttgatt cgtgatccct gttaaatgct ggatttgttt tgaaatcttc cgccctggat
    12121 gaatatcgat cgaaaaacgt gaagtgatcc gacctgattc aatcatgacg cagccaaatt
    12181 ggatgatccg atcttctttt ggattcgtcc cagtcgtttc gatatcaacg actgcataag
    12241 ttcttggttt caaaatatct tcctcttctc ctgcctctaa tgttcttttg aaattatact
    12301 acaaagtgca gaaaaatact ctcatcgctt tttaaagaaa atagacaaaa acgcccagac
    12361 acgcaaatgt ctgggcgttt attttttagt ataggttaat taagctttag taacgtttgt
    12421 tgcttgaggt ccgcgttggc cttcttcaac atcgaaagtt actgcttggc cttcttctaa
    12481 agttttgaat ccgtcgcctt ggatagctga gaaatgaacg aatacatcgt ttccgttttc
    12541 tgcagtgatg aatccaaatc ctttttctga gttaaaccat tttactgtac ctgtttccat
    12601 aataaaaatc ctccttgtgt atcaacacat attttgcaat tacttgaatg gtgtatatct
    12661 ggaagatgtt tatttgaaac aagctaccac agattaccga acaaaaacta ctcctatagt
    12721 atatcatacc gtttacaaaa aagctagagt ttttcgactt tttttattaa aaaaagtttt
    12781 ctcagcgatt acataaagtg aaaaaccgaa ctttttttaa aataaacgac atattgttca
    12841 gttggtgttt ttttgctctg atgttcttct ttaaactaaa aaaattattc tttctgacta
    12901 cttatcggtt ctgtttcaac ggtcgaggct ggggaagatt ctttagtagg tctgatgttc
    12961 tcatggtatt tctgatagcc tatgacagtt accagaagaa caaggcaaac caatacgatc
    13021 aacgagatta gtttttcttt tttgctcata tctatacctc ataaataaca ggatgatgtc
    13081 catcatattt ctgagtatga gtaaaagcaa aagctgcagc tgtagaatgt aagaaatgcc
    13141 agcaactttc ataatcacag gtcggacagt accaaaagta accaaatctt ggctgcaaag
    13201 accgttcttc tgtttcagga atcgcagaat caagatctgt tgtctgtaca taaacatttt
    13261 tgtacagacg tgtgcctgta ataggagacc gctgtgtttc ttcagcaaaa actggctgac
    13321 ttgttaaact tagggataga aacagcaaac accataacag tctttttttc ataaagacct
    13381 ccttatcgat aagtttttcc caattaaaat catatctcaa gaacgcccgt tattcacatt
    13441 ttgttcataa atgatgtttt ttgtatcata atcgtcaaaa agaggtcgtg accacgtcac
    13501 aacctctttt ttaaacggtg ttccagaagt agcttcttcg gaaataagtc gaaatttcac
    13561 aaaaatttga gaaataattt ttgtcaatct cttcttattt cttgaagctg accacttctg
    13621 tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc tgcggtatta gctcaaaaaa
    13681 cggaaaaata tgtgaaacta tttttccgtt tgtttctcta atactcaggt caaaacactg
    13741 atttcacaac ctcttttaat aacgaagaat cgctgtcaga ctcttttcat ctggcgcatc
    13801 tttttgatcc aaaataaaca ctttcccgcc attaatgatc acatcatctg ctaatgcatt
    13861 caacacttga cgccgatcat attctgtttc aggatcttca ccgaaaccat cagctaagtt
    13921 tgacgtagcg ataaataaat gtgacacttt tccttctttc gcagctggaa cgatatcaac
    13981 taactgatcg atgaatttct tatctaagag tttattgtat tcttttgatt cttgagctgc
    14041 gagttcactt gacagcttct tcgccccttc ttcgatatct ctaaaggata gctgcgcagg
    14101 agaagcagaa atcgcaacgt ctgcacaata atagggattt ttagcaattt ttttgaacat
    14161 cgtctgattt tctggtaatg caaataaata aatcggccat tgttcaggat tttcgaaaga
    14221 atccttcaag tacgtatcaa cagcttggta atagttgacc caatcgatct ctacttcttc
    14281 atcttttgga tttacgccat ggaaagttac tccatctcgg gctccgcctc ctccattaga
    14341 agagaagttc aaaccgccac ctgtcagttc agatcccaat gcagtaacga catcaaccgg
    14401 tgccccttct ggtagagata cttcttttac ttgtttgttg ctgacctcat acaatttcat
    14461 ggagtcgcgg ttcaatccca ataaataata agaatagttg aactgcccat ttttgataat
    14521 agccagtaaa taaggaagat cacttacata gtattgatcg tctaccggga tactcaaacg
    14581 atggatatag gtctggtcag cagttataat gaccgctaca ctcgtagttg cactacgcca
    14641 aaacgaagga tctcctaaca aattttcaat tttttcttgg aacggggacc agtcatgttc
    14701 ggaatatttt ttctcaaaac gtttctttgc ttctttcgca aaatttttga atttgatctg
    14761 atctttttcc acatcttgat gggcgacatg cgtattcagc atgatcgtga cgaatggtcc
    14821 tttagcgcct tctgaagtca attgtgtcaa taattcttta tccttttttg ccataaacgt
    14881 acccctccta aaaaaatcat tcctttgttt ctatctaaag tctaccacaa cactattttt
    14941 ccatacaata aaacgtatct aaaaaaataa aacggtttct acggttttaa atttagctat
    15001 ttttttaagt tatcttcata atttcctcaa ttatttagcc ccagtcttca actattcttc
    15061 atgaaatgct gataaactaa gaaaaagaat aaaagaggtg gatcttatgc atttgattat
    15121 gattttcagt ttattaagta ttatcagttt atttgctttc agcgcttccc gcttattaga
    15181 tgcggaaaca cagcctattc ctgtaaaagt acgtaaaaac gaccgctaaa cttccataac
    15241 gcgcgttctt atcctaaagt tttttcagaa acattgaaac tctctcccct ctctgataaa
    15301 attttaattt gtagcgaaac aaattaatta caaggagatc ggatatgtcc ataaaaaaaa
    15361 cacgtctagt cctattattg gctagtctca ttctttgcct tgcttctttt ctaccagctg
    15421 ttgctgtgca agctgaagat acgttcaaag taaatgcaaa agcagctttt gctgtagatg
    15481 cgcaaagcgg taagattctt tatgatcaag atggagaaaa aaccatgggg atcgcttcaa
    15541 tcacaaaaat catcggtctt tacatcgtcc ttgatcaagt aaaagaagga aaactgtcat
    15601 gggatgataa agtttcgatt tcagattatg cagaaaattt gagtatcacc ccagatttat
    15661 cgaatgttcc tttgcataaa gaaaacacct acactgtcaa agaattattc gactctgcaa
    15721 tcatccagtc agccaatgct tcaatggtgg ccttggcaga aaaaatctct ggcagcgaag
    15781 ctaaattcac tgaacgaatg aaagaacaat tgaaagattg gggaatcaaa gatgcaacaa
    15841 tcgttaatgc atcaggattg aacaactctt atcttggcga aaaccgacca gaaggcactg
    15901 gagaaaacga cgaaaatcaa atgtctgctc aagatgtggc catcgttgcc cgccatttga
    15961 tcttggattt cccagaaata ctggatgtat caagtactac gacccaaatg tttggcgaaa
    16021 atacgcaatc accagtggaa atggtcaact ggaactggat gctcccagga ttcgttaatt
    16081 acaaggaagg cgtggacgga ttaaaaactg gtacgactga gcttgcaggt gcttgtttcg
    16141 ttggtacgat cactaaaaac gatcaacgga tcatcactgt cgtattgaat gcggacggcc
    16201 atgcggaaaa cccgagtgcc cgcttcaatg aaacagaccg tttgatggac tactgctatg
    16261 acaattggtc agaaaaagag ctaggaaaag ctaacgcaag tattcctagt ctaaaaacca
    16321 ttgatgtaaa agacggaaaa gaatcaacag ttcctgttgt gcttaaaagc cctgtaaaag
    16381 tatgggtacg aaacgatatg gatactggca aattaacgat cacgccaaca cttgacaaga
    16441 agcaagtcac tgacaatgag atccaagcac ctatggataa aggtacaaaa atcggtaccg
    16501 ctacgatcac cttggcagac gataaattag gctatttaga agacaacaat gcaccttcaa
    16561 cagagatcat tacggataaa acagtggaaa aagcgaatat attcgtgatt ggctggcgac
    16621 gcgtcgcaga atttttcggc aacttgtttt aattgaacaa taagaaaaac at
//
LOCUS       DACDRN010000052        16615 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDRN010000052 DACDRN010000000
VERSION     DACDRN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.052"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..525
                     /gene="scm"
                     /locus_tag="IWJ80_001987"
     CDS             <1..525
                     /gene="scm"
                     /locus_tag="IWJ80_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9461698.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
     gene            complement(680..1666)
                     /locus_tag="IWJ80_001988"
     CDS             complement(680..1666)
                     /locus_tag="IWJ80_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9461699.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1689..2255)
                     /locus_tag="IWJ80_001989"
     CDS             complement(1689..2255)
                     /locus_tag="IWJ80_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP9461700.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2269..2685)
                     /locus_tag="IWJ80_001990"
     CDS             complement(2269..2685)
                     /locus_tag="IWJ80_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP9461701.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3142..5076)
                     /locus_tag="IWJ80_001991"
     CDS             complement(3142..5076)
                     /locus_tag="IWJ80_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9461702.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5378..6313
                     /locus_tag="IWJ80_001992"
     CDS             5378..6313
                     /locus_tag="IWJ80_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP9461703.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6403..7506
                     /locus_tag="IWJ80_001993"
     CDS             6403..7506
                     /locus_tag="IWJ80_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9461704.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7519..8472
                     /gene="menA"
                     /locus_tag="IWJ80_001994"
     CDS             7519..8472
                     /gene="menA"
                     /locus_tag="IWJ80_001994"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9461705.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9407..10753)
                     /gene="gor"
                     /locus_tag="IWJ80_001995"
     CDS             complement(9407..10753)
                     /gene="gor"
                     /locus_tag="IWJ80_001995"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP9461706.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10887..12128)
                     /locus_tag="IWJ80_001996"
     CDS             complement(10887..12128)
                     /locus_tag="IWJ80_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9461707.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(12146..13069)
                     /locus_tag="IWJ80_001997"
     CDS             complement(12146..13069)
                     /locus_tag="IWJ80_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9461708.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13290..15782)
                     /locus_tag="IWJ80_001998"
     CDS             complement(13290..15782)
                     /locus_tag="IWJ80_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9461709.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15789..16256)
                     /locus_tag="IWJ80_001999"
     CDS             complement(15789..16256)
                     /locus_tag="IWJ80_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP9461710.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(16392..16465)
                     /locus_tag="IWJ80_002000"
     tRNA            complement(16392..16465)
                     /locus_tag="IWJ80_002000"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16429..16431),aa:Arg,seq:acg)
     gene            complement(16523..16596)
                     /locus_tag="IWJ80_002001"
     tRNA            complement(16523..16596)
                     /locus_tag="IWJ80_002001"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16560..16562),aa:Pro,seq:tgg)
BASE COUNT         4907 a         3477 c         2956 g         5275 t
ORIGIN      
        1 acatcagctt caagtgacag tacagatact acctctacca caagtgatag cacggacacg
       61 tcagcttcaa gcgacagtac ggatacatct accacaacaa gtgatagcac ggacacgtca
      121 gcttcaagtg acagtacaga tactacctct accacaagtg atagctcaga ttcatctacc
      181 actacaagtg atagcacgga cacgtcagct tcaagtgaca gtacagatac atcctctacc
      241 acaagtgata gctcagattc atcaacttca agcgatagta cagacacgtc gtctaccaca
      301 agcgatacac gtcatgcaag tgtctccttc tcaaaaacaa gttcggataa caggacgaac
      361 tttaacttaa ctaataaaac aagcaatgac aatggaaaca aatcaaaaag agcacttcct
      421 aaaactggaa gtcaatcgaa taattggata acactcgcag gcgttatatt acttgtaatc
      481 ggtttaagaa tcacttttat gagttataag aaatcgaaaa ggtaaaaaaa tattcaagat
      541 aaaaaagagc aacgtcgcta acggacgttg ctctttattt ttgcttttta tcataaatac
      601 aagaactaca attcaatttg tcaaaaaaca cgtagatttc cttctgagaa atttatttac
      661 gtatcgaacc tttccctatt cagtctttgc gatccaaaat cagttgggta agggaaaaga
      721 gattctcttt tgttcgaaga gaggtggcag gtaatttctt tatttccttc aaagcctttt
      781 ctgtgtaaga ggccgctaac ttcctagctt cttccacgcc tcctgtatgt tcaacgattt
      841 tgcgtacggc gtcgacatct tcttctgtca ttgccgcttt cttctctaaa taaggtagta
      901 gctttggttt gtttttttgc aaagagtaga tcaacggtaa agaatagacc ccttgacgca
      961 tgtcttctag tacaggtttg ccgatttcct ggctctcttt tgtataatct aaaatatcat
     1021 cgataatctg aaaggccatc ccgatagaaa gtccgatatc tcctgctttt tttgcgaagc
     1081 gctcgcttgt tccactttca aaagcgccaa cagaacaact taattggaac agttcagctg
     1141 tcttacctga aatattttcc aaatattctt ttacagaaag ttcataatta tagcgatcat
     1201 ccatctgccc taattcgcca ttcaatactt tttccatact gcgagaattc aattggagac
     1261 ttttcaagga agtggagtaa tcagacaata gtttaaaaca gcagacaaat aaatagtctc
     1321 ctgcatagac agaaacgaca ttaccaaact gactacggat attaggctga ccacgtcgca
     1381 gatccgcttc atcaacgata tcatcatgga tcagtgtggc cgtatggagc atttcgatag
     1441 aagctgccaa agcgatggct ttgtctcgat cttgctctgg tccaaattgt gagaacaata
     1501 gctgataggc aggacgcaac agctttccgc cggcatggat cgtttctttg attgcttgtt
     1561 cgacaggttt gtttttcagg cggacagttt cttccatccg ttttagtgtt tctgtcaatt
     1621 ctttacttaa agcaggatag ttttcccaca ttggatgtag cttcataaaa aaactccttg
     1681 tgtgacgatt attttttggt aaacattggg ctccattttt gtttggttcc ctgctcataa
     1741 tctttttgga atcgttgaag tgtctgcaca tgtcgtaaaa gataattcgc tgcgatcccg
     1801 attgcaatcc ctgataaaat ccctaaaact gaaaggactg gcaagtacaa catgactgtc
     1861 catgattgtg caatgaaaga agccatcaaa agctgtccga cattatgcat gaaaccgccg
     1921 gccgcactaa ttccgatgat actgacacgt tttggtccta attgcttgac caataacatt
     1981 ccaaaataac taagcaaagc accactcata ctataaagga atgtggaaat cgttccgcct
     2041 agcaaagtag tcaggaatag tcgtagccaa agtagtaaga agctgtctcg ttttttcatg
     2101 gtaaaaatgg caacgatcgt gattagattg gctaagccta gttttgcacc aggcgcaaaa
     2161 gaaaaaggat aagggatcat attttccaat aaaccaatga tcactccttg tgcaacaagt
     2221 agcgaaatat aaatcatttt ttgtaattta ctcatggtgt gtgaaggtct aataaatcag
     2281 actgtcttcg tcacctccat ctgttgaacg gacttcgatc actaatttat gtggcaaaca
     2341 gacgatggtt tcgccatttt tcgttgccca gcctcgacgt acgcagatct ggtcatcgca
     2401 atctgcttct tttatccgta ctcgatcgcc atctatctcg atcaagttat aatctccgtg
     2461 ttcatctttg taagtatatg tgttgctctc ttctccagcc atcaaaggga atgttttgat
     2521 ttctgtgcca tccacacgca gtaccgcttc tttttctgct gttgcggtgt tttgctgaag
     2581 accaaaaatc atgactggta aaaaagaaag aacagtcaaa agcaaaataa taaaaacatc
     2641 ccaaggtttg agcagacttt tttcagcaaa ttctttaagg ctcatttttg ttctcctttt
     2701 tccagattaa ctcacccaat tttagcataa aaacaagagg ttgtgacacc tgtgtcttgc
     2761 tttgagtaat aagaacgaag aatagaaaac agctttccat gttttctaat tctttgggct
     2821 tattaccgag aagcagcagg tggaacaccg tttataacaa gaggttgtga cagaagtggg
     2881 caacttcaag aaataagaag gaatttacga aaattacttt tcaagttttt gtgaaatttc
     2941 ggcttatttt tcgaagaagc tacttctgga acaccgttta taacaagagg ttgtaacaga
     3001 ggttgtgaca cctgtgtctt gctcatggag gcttatctcg ggcatcttat tcctttaatt
     3061 ttacataaaa aggaggctgt gacggtttgt ctcagcccct ttctattgaa atcttgcatt
     3121 caaataacgt tctttatcta tttatttctt gccgtactgg gaacttggtc taccgtacca
     3181 ccaattacct agtgtacgtt gtagccatgg aattacccag cgatccaacc caatcgcacg
     3241 tccggaaccg ttcatcaatg cgaaagcaac aaaaatgaac caaatgttta cccagtaaaa
     3301 catgcctgat aagcagaacg tgatcaccaa tacaatcgtt gtcgcactgc atagccatgt
     3361 gaataatcca gcaatcaatg ctaatgccaa ggcaatttca aagaatacca tgaatttttg
     3421 gaagaacatt gctacatcat tgtttggcat gatcacttgc atgatctttg tgaaccagtc
     3481 tggtgacttc tcaatcacag ccattggttc ttcaccgtat gcataactca aaccaaaatg
     3541 tgcagcttga gtcgctgctt ctccggcact tgcccctgct ccgctggcag ctgtggttgc
     3601 atcaccagca gcagaagcac cactggcaac atctgttaca ttgtattccc atgtccaagg
     3661 gaacacactg tttccagtga accatgatcc ttcaccaaac caagtacttg gtttgaacac
     3721 ttcaccattc ccaatgattt ttttggaagc ttcaacgaac caaactaaac catagaaaat
     3781 acgaagtggc acactccata agacattgcc gtaacgagaa gaatgtcctc gagtcacgtt
     3841 gcgatcatct ttgatatgga aaaactcatg cattaaatat tggaacatat aataaccaga
     3901 acgaatatca aagaagtatt tcaaattcac gatatgtttc attacgatag ctaaaaatcc
     3961 gcttaaatgg atcttatcaa ataggttagc taccccccat ttggcaccaa tagaaaccat
     4021 aaatccttga tagtttcctt taaatttatg cttttctccg cctttgatcg aagccacgat
     4081 attcgctgcg gctgtgtgtc ctgtttgttc agcagcttga acgatctgcg gtgtcggtgt
     4141 tcctgggaat tcttcataaa agactaaatc accaatgata tagatgtttt tatcttcgta
     4201 gccttttgcc tgcatatatt cattggcgat caaccgctgt ccgcgtgctg attctaatcc
     4261 aaagtcagca gcgtcagaag ttcctttgac accagctgtc caaatcagtg tgtgagtcgg
     4321 aatgttgcgt ccgtctttca atttgatatg atcagcagcc acttctacga ttggtgcatt
     4381 caataataat tccacgttgt gcttacgcaa atagcgttca gcttttgcag catcatttcg
     4441 atccaacata ttcaagatcg tcggcattgc ctctacgacc atcagtgtga tttcgtttgg
     4501 atctaattta taatctttgg ctaggcgatt tttccagtcg atcagttccc caatcatttc
     4561 aataccggta aatccagaac cacagacaac gaaagtcagc atggctttac gcagttcatc
     4621 atctggttcc aatgcagctt ttgctactgt cttctcaata tgttcccgaa tccttacaga
     4681 atcttcaaat gaccaaaggg tgaatccgtg tgctttgaca ccaggtgtac caaagtcatt
     4741 cggctctccg cccattccaa ggatcagctg atcaaaagga taggaaccta atttggtctt
     4801 gacgattttt cgttctttat cgataccgat tacagtgtct gtgacaagtt gtacattctt
     4861 ttgacgagca aacaaatgct gcagatcgta ttggattgct tctggttcta cacgtccgcc
     4921 agctacttca tgcaactcgg tcatcatagt atggtacgaa tgacgatcga tcagagtaat
     4981 tgctacatct tcttttttca gtttctttgc caagtatttt gtagctgaaa caccggcata
     5041 tcctgctcct acaacgacaa tattttgctt tgccattgat catccgctcc ttatagacta
     5101 tctatataat gaatatatga taattctttt agtttgatac ttattacaca atctccatat
     5161 attatataga tgttctgttc ttttgtctaa ctccttttgg atttttttca aattttaaaa
     5221 taaagcgttt tttcacgaaa tttgctgaaa gaaaacgttt gaaatattac atgggttatt
     5281 tatcaaaaat agttgactta taggctaaaa agattagaat gtataatgaa tcacaaagaa
     5341 aaattgtgga ctagaaatgg aatggtgggg tattaaaatg aagaaaaaac gttttgtaac
     5401 tggatttgcc gtattagcat tttcagctct agtgttaggc gcttgcggcg cagatgacaa
     5461 cggaagctca aactcttctt ctgaatcatc tacagcacaa tcttcgactg caaaaagcac
     5521 aacagaatca tctgcaaaag ttgtagctgg cggagatttg caagacggca catataaatt
     5581 agaagaaaaa aattattcaa atggttaccg tgcagtcttt gaaatggttg taaaagacgg
     5641 caaaatcact gaatcaaaat atgataacgt caatgaaaac ggcgaatcaa aaacaaaaga
     5701 tgcagaatac aacaaaaaca tggaagcaaa atctggcaca aacccagaaa aatacattcc
     5761 tgagttgaat gaccaattct taaaagcaca aagcgcaagc ggtgtggaag tcgtaactgg
     5821 agctactcac tcatctgaaa gcttccaaaa ctacgcacaa caattgatcc aagctgctca
     5881 agctggtaac acagatacaa tcgaaatcga caatggtgct gatttgaaag acggcactta
     5941 caagcttgaa gaaaagaact actctaacgg ctaccgtgtg caattcgaaa tgacagttgc
     6001 tggcggcaaa gtaactgaat caaactttga ttatatcgac aaagacggta aatcaaaaca
     6061 agatgataca gaatataatg aaaatatgaa agcaaaaagc ggtacagaac caaaaactta
     6121 catccctact ctgaacgacg aattcgtcaa agcaatgggc gaagaagatg gctcacctgc
     6181 taatgtagaa gttgttacag gtgctacaca cagctcacat tcattcatca tgtacgcaca
     6241 acaattagtg aatgcagctg aaaaaggcga cactcaaaca atcgaagtag ataatatcgt
     6301 aacagaaaaa taatcacata atcatactaa taaaagttgg agggtgggac gatagccaga
     6361 ttaggcagtc gtctcacctt tcaaaatgaa gagaggaaaa agatgcggaa aaaactacta
     6421 ttttccttaa tcacactggg catctctttt atggtgctaa ctggctgcag caactcagca
     6481 aataaaacaa ccactgaaag ttcagcagct accaagatat tgaaagatcc atattctgat
     6541 gaacagttct tgttagggac ttacgttcgt atccgtgtgt atgatgaagg caaagaatca
     6601 gcattgaaac ctgccttcga tcgagtaaaa gaacttggtg ataaaatcac gatcaatcaa
     6661 aaaggttctg agatcgatga agtgaatgag caagctggga tcaaaccagt aaaagtttct
     6721 gatgacgtct acacgttagt caagcgggcc tacgaatata gccaagattc tcaaggcgga
     6781 ttcgatatgg cgattggtgc aatcacacaa ttatggcgaa tcggttttga tgacgccaga
     6841 aaaccttccc aagaagaaat cgatcaggca ttgaaattag tggattatca taagatcgaa
     6901 ctaaatgaca aagaaaaaac tgtttatcta aaagaaaaag gaatgatcat cgatttaggt
     6961 gcaatcgcca aaggttacat tacggacgaa gtagtgaaag tcttgaaaaa acaaggagtg
     7021 accactgcca tcgttgactt gggcggtaat gtctatgtct taggccacag cccaagaggc
     7081 gaaaaccaag actggacagt cggcatccaa gatccgaaca tggctcgcgg atctgtttta
     7141 ggttctatta aagaacggaa caaaacatta gttacctctg gaatttacga gcgttattta
     7201 gaagtagatg gcaaaaaata ccatcatttg tttgatccaa aaacgggtta tccgttcgac
     7261 aatgatatcg caagtgtcac gatcataaca gataaatcaa ttgatggtga cgggttgtct
     7321 acagcagtct tttcaatggg tgtgaaaaaa ggactggaat acgtcgaaag cgaattgaat
     7381 aacgggacgg aagcgatctt cgtgacaaaa gacgataaag tatatgtgac cgatccaatc
     7441 aaagacacct tcaaattatc ggaggactcc cattacacga tgggtgaccg ttctgaacta
     7501 aaataaggag tacgaaccat gacactaaaa atctttttag aagtagttga aatacgaaca
     7561 aaggtggcta gtgtatttcc ttttgcggta ggggtattat tctctatcgc tttttttcat
     7621 gagatacatt ggctaaacac ttttttattt tttattggaa tgctagtttt tgatttagcg
     7681 actaccgcta tcaataatta catggattat gaaaaggctc attctgaagt atataagtat
     7741 gaagaaaatg tcattggccg cagaaatcta tctcctgtat tgattcgaaa catgatttta
     7801 gctatgattg catttgtttt tgttatcggc gttattctaa cgatcaatac gggttggctt
     7861 ttacttttga tgggatttat ctgttgtttt attggagtat tctatacatt cggaccgatt
     7921 cctttatctc gaatgccttt aggggaaatt ttcagtgggg tgactatggg ccttggaatt
     7981 tttgccatgg tcgtttatgt caatacattc ggttcagatg tctatgatct gacagtcaac
     8041 ttttcatctg gacaatttgc tttggtagga aatctttggg ctatagcagc aatcgtatta
     8101 gcttccttac ccttggtgtt tacgattgcc aatatcatgt tggctaataa tttgcgggat
     8161 cttgaccgtg atattgaaaa tcatcgctat acactagttt tttatatcgg acgtcctgcg
     8221 ggaacagtct tattccagct attgatgtat gcttgctatt tcgtgatttt aatggggttg
     8281 ttgagcggta tctatgaatg gccgatcttg gtcaccttct tgaccttgcc aattgtgaag
     8341 aagcatttgg atcaattcaa gcaaacgtta ccccagccta tcagtttcag ctattccatc
     8401 aaaaatatgg tcttattcaa tgccagttat gtattggggc tattactaac aatcattatg
     8461 caagccattt aacagcatga agaggttgtg aaaaaactgc cctgcatcaa gtaataagaa
     8521 cagccaaata aaaatagctc cttatatttt tcgtttggtt gggcttatta ccgcagatgc
     8581 aagcttttga acaccgttta tcaggaggtt gtgaaaaagt cgcttagttt cgagtattaa
     8641 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgagaa
     8701 actggctttt gaacaccgtt tatcagaggt tgtgaaaaag tcgcttagtt tcgagtatta
     8761 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaga
     8821 aactggcttt tgaacaccgt ttatcagagg ttgtaaaatc agtgttttgt cctgagtatt
     8881 agagaaacaa atggaaaaat agttcctcat attttgccgt tttttgagct aataccgaag
     8941 gacagctgat tgaacactat ttgttcggaa aaagtgacta actatgacac aatttttttc
     9001 acagtccctt ttttcacttg atttgattcg taccagcgaa cttgacgaga actttcttgg
     9061 tttggtaact atatggtatt cttttaacat cctcgtttgc ttcttctaat ttatttatac
     9121 aaagtcacac ttttttaaac atctgttctt cgatacattt ctatttcttt taaacatcga
     9181 taccaatatt ttgaacttaa gagtgcatac caaatggatt catttactct tcgtcagtta
     9241 cgaagataat caaaatctcc aacttcccta cttatttcgt tttgagaatt atcgaagcaa
     9301 tcttacataa ataactaaaa aacaaaaaac cagaaaatca gtatactgat tttctggttt
     9361 tacatggttt gttcaataaa aagctgttgg aactgttaat attaaattat cgcatcgtta
     9421 cgaattcttc cgctcctgtt ggatggatgg ctactgtatt gtcaaagtct tctttagttg
     9481 cacccatctt gatagccacg gcaaaacctt gcagcatctc atcgacacca acaccaatag
     9541 catgtaagcc gacgattctt tcttcttttc cgacacagat caatttcatt tcgcattttt
     9601 ggcgataatc atttaatgca aagtacattg gggtaaagcg tgaacgataa actttcaatt
     9661 cattttcgcc atatttttcc aatgcttctt cttctgtcaa accaattgtt gcaattggtg
     9721 gatgcgtaaa gacgactgtc ggaacaagtt tatagtcaag ataagaatct ttctttccat
     9781 taaataaacg ttctgataaa cgtctaccag ctgcgattgc aacaggagtc agatctaatt
     9841 taccgatcac atcaccaaca gcaaaaatat gttcatccgt tgtattttgg tatttatcta
     9901 ctttgataaa cccttttttg tccaattcca catttgtatt ttccagacct aatttttcgg
     9961 tattcggtac acgtccaccg gcaaaaagta cacaatcacc agttaaggtt tcaccatttt
    10021 caaatagcac cgtatattcg tcattgtttt tagtaatttc tttagcaata tgatgtgcgt
    10081 aagtttttat tccagcttct tggtacattt ggatcaaatt gtccgaaagc attttatcaa
    10141 acgttctaag tggtcgttct tttcgatatg cccacatgac ttcactacct aaaccacaaa
    10201 atacacctga aagttcagcc gcaatgtatc cagcacctaa aacgattgct ctcttaggta
    10261 actctgttaa ttcaaaaaat ccatttgaat caattgcgta ttctccacca ggaatggtca
    10321 ttttacttgc acggcctcca gtagcgatca agattttagg agcacgatag gtttcaccgt
    10381 ttacttcgat cgtttgacta tcgataaaag tagcgtaacc ttttatagcc tcgacatgat
    10441 tgctatctaa tcctctttga taagcaccgt gtagaaaatc aatatatttt tctcgtcttt
    10501 ctactagacc ggcgaaatca acatttttta aatctgcttg gataccgtaa cttgaggcat
    10561 ctcgttcaat tgtttccaaa atcgtacttg cttgccacat aactttttta ggtacacaac
    10621 cgacgttgac acatgttccg cccaattcat tcgcttcaat cagcaaaact tttgcgccat
    10681 gcatccctgc tctgtttgct gaagcaatac cgccagagcc gccaccaatt acgatgtaat
    10741 catatatttg cattttgttc ctccttattg aagagatcaa tcaaaacctt tatttaacga
    10801 tcctttttct acagaaaaca agtgtaacac ttttcttgaa ttttgtatgc gaagatgctt
    10861 aaaacaggga tatatcagct ttttccttat ttacgttttc ggatcatatc tccaggtttc
    10921 accggctgtg cgactggcat agttaaaatc atcattggat ttggtgctcg gtcaatggat
    10981 tcgccttctt cattatgcat aacttcaacg gtttgactga aatgactgaa tcctggacca
    11041 taaaattcga tttcatctcc tacagaaaac aagtttctct gacgtatcgt agcaatctgt
    11101 gttgcctcat cataagataa tacttcaccg acaaatttat atactgggat tttacgacgt
    11161 tcaccgaata attgttcgtt ttcagttggt gtattgtagt aaaaacctgt agctaattct
    11221 cgttgtgcta ctttccataa ctcgtcgatc cattcttgct tgcagacata gttttcagga
    11281 tcttccatat aactgtctac agccgcttta tagacatttg acaccgtaga tacataatgg
    11341 atcgatttca ttcttccttc gatcttgaag ctgtctacgc cgttttcaat caattctgga
    11401 atatgttcga tcattgacat atccactgca ctcatcgaga attcttcttc cacttcgcct
    11461 gtatcagtta aactgtttcg ttcttgacca aaaggcatat cgaacaatcc atacttccag
    11521 cgacaagatt gagaacatcc gccacggttt gcatcccgca tcgacatatg gttggacaat
    11581 gtgcatcgac cagaataaga gatacacatg gcaccatgga taaatgcttc gatttcggta
    11641 tccgtatttt tacgaatctc tgccacttcc tccattgaaa cttcacgagc taaaacgaca
    11701 cgttccaacc cttcttcttt ccagaattcc aatgtctcaa aattagttgc tgaagcttga
    11761 gtggataaat ggatcggcag acctggggct tctgtcgcac aaatttcgat caaagctgga
    11821 tcagaaacaa tcactgcaga aataccgaca tcacgcaaac tgcggaaaaa ttcccctgct
    11881 ccatcttcat ttccttcatg ggtgaccata ttagcagcaa cgtatacctt cgcatcatgt
    11941 tcttttgcaa aagcaactcc ttcagccatt tcttcataag taaaattacc tgcacggctt
    12001 cttaaaccat atgcattccc gccaatatat actgcatctg ctccataatg gatcgcggtt
    12061 tttaactttt ccagtgttcc tgctggcgct aggatttctg gtcgtttcaa ttttcttttt
    12121 actgtcattt ctttcatcat actccttatt tgatagcaga aggatcaata taaaagaatc
    12181 ctgtatctaa ttcacggttt tcaggatgca atttactgat ttcgctagtc attttttccg
    12241 ctttttcttg atcccagcta ccttctaaaa tttgtttttt tgcttcggcg aatagtcgag
    12301 tgatgtttac gaatttttcg cctggagtaa ataatccgtc caatttccat gtacagtaat
    12361 gatgctgata aagttcattc aattcattcg agagattcag atcattgctg gcaaaaatat
    12421 gtgtgccatg actatcttca taaatagaat aatgtgtctc ttctttttta ggttccgaga
    12481 taaaaagacc tctttcacga tctttttgtt cgtcttgttt cgtatagtta tagtaattct
    12541 gtagcagtgg acgtttggat tgatggatgc atgtagcacc atacacaagg gtttctacag
    12601 gaatatcgag tttttcttcc atcgccttca tctcttcaaa aggaacttca cgagcaagta
    12661 ctgctccaac tgcgcctttt ttcgcccaga aattgatttg acgtgcgctc gttactaatg
    12721 tttcagcatc ataaatatac ggtatacgca attcaggatt ttttttcatc gtataaataa
    12781 tacctgtatc accaactgtg atcacatcta cgtttatttc ttttaaaaaa gccaaatatt
    12841 caggtacatt tttcattttt tcaggatgaa tgatcccatt tacagcaata ttcgcttttt
    12901 taccatactt gtgtgctaat tctaccaatt gtcgctgttc ttctcgtgta aatgaagcag
    12961 gcagacgcaa tccaaattgc tcttcaccga aatatagtgt atctgcacct gcagcaagca
    13021 actgttctgc ttgttcaata gtttctactg tggttatgag ttcgatcata attttctcct
    13081 cctacaactt cataatagta gcatagctct aaaaactttc caatacttct tctatttaaa
    13141 gtcggatttt tgaaaattat ttcacaaaaa atcaaaataa agttcgatat attctgtcca
    13201 aatataaaaa gagattgtga caaaaatctt gtcacaacct cttaatcaag tcttactatt
    13261 ataaaaattc agctacacag gccaagtaac tatagggaac ctactgtttc ctttttaggt
    13321 ccgcgaacat tcaatgtgat cttacctttt gaagcaccta ctgttacgtg gtcacctaaa
    13381 cgaatttctc cgccaagcaa agcctcgctc aatcgatctt caatttcttt ttgaagtgcg
    13441 cgacggattg gacgtgcacc atattcagga tcaaatccag ctttcccgat cacatcgatt
    13501 gcagatggtg tgattttcac ttgaatatct tgttctttca atcgtttgat aatcgctttg
    13561 ctcatgattt ggacgattgt atggatttca tctttactca atgaatggaa gacgacagtc
    13621 tcatcgattc gattcaagaa ttcaggtcgg aaagctttct ttaactcttc taagatacgt
    13681 ttttgcatgg ctttgtgatc tttagtaaca tcttggacat tgaatccgac atttttttct
    13741 tctcgaattt gtgtcgctcc gatatttgaa gtcataatca tgattgtatt tctaaagtcc
    13801 acttttctac ctttagaatc tgttaaatga ccatcatcca atacttgcag aagtaaattg
    13861 aaaacgtctg gatgtgcttt ctccacttca tctaagagaa tcactgaata tggtttttga
    13921 cgaatttttt ctgtcaactg tccaccttca tcatacccta cgtatcctgg aggagacccg
    13981 attaatcggc ttgtactgta tttttccata aattctgaca tatcaacacg gatcagggca
    14041 tcttcacttc caaacatgac ttctgaaagt gctttggcca attctgtttt tccgacacca
    14101 gttggtccaa ggaacatgaa tgaaccaatt ggacgatcag gatctttcaa tccgcttcgt
    14161 gcacggcgga tcgcacgtga aactgctttg acagcctcat cttgaccaac gacacgttca
    14221 tgcagaagtc cttccagttc taacaagcgt tcgctttctt ttttctccaa ctgctgtaga
    14281 gggacaccag tccactccga tacaactgta gcaacatctt cttctgttac acgatcagaa
    14341 taaccttgct cctgttttgc ttcaattgca gaaacttttt gcagcttttt acgtagtgct
    14401 ttttcttgtc tgcgtaattg agcagcattt tcaaagtctt gtttttggat cgctgcttcc
    14461 ttttcttgta ctagttcatc gatttctagt ttgatcacag ttgattttgt taagtgatcg
    14521 gtttgatcca aacggacttt cgctgctgat tcatcgatca aatcaattgc tttatcgggt
    14581 agttgacgag aagtgatata acgaacagaa agattgactg ctgctcgtac agcttcatct
    14641 gtgatctcca ctccatggtg ctcttcatat cttgtacgta atccttgaag aatcacttct
    14701 gcttcttcag gagttggctc atctacttga atgcgagcaa aacgtcgctc taatgcagaa
    14761 tctttttcga tatatttttg atattcatct agggtcgttg ctccgatcgt ttgtaattct
    14821 cctcgagcta acgccggttt caagatattc gaagcatcaa ttgctccttc tgctccacct
    14881 gcaccaatta aggtatgcaa ttcatcaatg aataagatta cttgaccatc attatagatt
    14941 tcgtcgatga cctttttcat tcgatcttca aattcaccgc gatatttcgt accagcaacc
    15001 aaagatccca tatcaagcat catcaggcgc ttttcttgca tgtcttctgg cacttcacct
    15061 aaaatgattt tttgagctaa cccttcagca atggctgttt tgcctacacc aggttcaccc
    15121 accagtacag ggttattctt tgtccgtcgg ctaagaatct ggataagccg tttcacttct
    15181 ctcgaacgac caacaactgg gtccagcttg ttttcttttg ctaatttcgt caaatcacgt
    15241 gctaatgaat ctagcgtagg tgttccttgc ggtgtttgcc gtttatcttg tacatttaca
    15301 tttctacgac gattcattcc ttttccagca gaatcaccag ctagccccat tttttgtttc
    15361 agaacttgac gggcttttga aaggctgata cctaagttga tcatgattcg agtagccatg
    15421 atactttcat cttttaataa gctcatcaaa atatgttctg ttccaacttt ttgagaacca
    15481 gttttcttca cttcgctgct agctaaagca aaaacttgtt tcattcttgg tgaatatgga
    15541 agaaagactc ctttagggta agaacggata ccaccataac caatcagatg ttcaatttct
    15601 tcataaattt ccgtttcatt caaatccatt tcacgtaata cttttccagc aattccattt
    15661 tgctcgacca ctaaggctag taaaacatgt tctgaactaa ctgattgatg tttcaaccgt
    15721 tttgcttctt cttgtgcaat ttctaatact gcttgggctc tttcagtaaa aagttcttcc
    15781 atctaatctc acttcccatc ttcataactt aaacgttcca gaaatgctcg gagaatccga
    15841 gcacgtgtat gatcttcata ttcattgaaa ttcaatgtgt tttttgcaat tgcactcaac
    15901 atcaagtttc cttctttttt tgtaattatt tggtcttcat aaagtttttg aataatagca
    15961 aaagcatttt tctcactgat tgtatcatca aataactgat ttatctgctc aagtaattgt
    16021 tttttatctg aaatccttac ttttgcaatt cgtatataac caccaccacc acgtttgctt
    16081 tctactagat aaccacgttg gatggtaaaa cgggtattaa tcacatagtt gatttgtgaa
    16141 ggaacacagt taaagaggtc tgccatttca atccttcgaa tctcgatcat ttcactattt
    16201 tccagaattt tctttatata tgattcaatt aaatccgagg tattctgatg tgccatctta
    16261 ttcattccct ttgactaata ttgaccttaa ttataacgaa tttgataata gaaagaaaga
    16321 agaacgcttt ttgttaaata aatcctttag aatcaacaaa aaaaaatacg gaatatacta
    16381 ttccgtatcg acgcgcccaa gaggagtcga acccctaacc ttttgatccg tagtcaaaca
    16441 ctctatccag ttgagctatg ggcgcatatt caatttaaaa aaattataaa tcatttgaaa
    16501 acaaaaagca agataaaaaa aatcgggaag acaggattcg aacctgcgac cccttggtcc
    16561 caaaccaagt gctctaccaa gctgagctac ttcccgttta aaaaataaaa aagac
//
LOCUS       DACDRN010000053        16215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDRN010000053 DACDRN010000000
VERSION     DACDRN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.053"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..175
                     /locus_tag="IWJ80_002002"
     CDS             <1..175
                     /locus_tag="IWJ80_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9461711.1"
                     /translation="FFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNERIKQKLNWQ
                     SLVQFRKTTVTVV"
     gene            364..1032
                     /locus_tag="IWJ80_002003"
     CDS             364..1032
                     /locus_tag="IWJ80_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP9461712.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1232..1618)
                     /locus_tag="IWJ80_002004"
     CDS             complement(1232..1618)
                     /locus_tag="IWJ80_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461713.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1590..2972)
                     /locus_tag="IWJ80_002005"
     CDS             complement(1590..2972)
                     /locus_tag="IWJ80_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9461714.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3128..4315)
                     /locus_tag="IWJ80_002006"
     CDS             complement(3128..4315)
                     /locus_tag="IWJ80_002006"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP9461715.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4322..5353)
                     /locus_tag="IWJ80_002007"
     CDS             complement(4322..5353)
                     /locus_tag="IWJ80_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9461716.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5369..6823)
                     /locus_tag="IWJ80_002008"
     CDS             complement(5369..6823)
                     /locus_tag="IWJ80_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9461717.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPTLSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVASASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            6998..7903
                     /locus_tag="IWJ80_002009"
     CDS             6998..7903
                     /locus_tag="IWJ80_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9461718.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTGESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7994..8671
                     /locus_tag="IWJ80_002010"
     CDS             7994..8671
                     /locus_tag="IWJ80_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9461719.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8952..9848
                     /locus_tag="IWJ80_002011"
     CDS             8952..9848
                     /locus_tag="IWJ80_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9461720.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9903..11273)
                     /locus_tag="IWJ80_002012"
     CDS             complement(9903..11273)
                     /locus_tag="IWJ80_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9461721.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11511..12257
                     /locus_tag="IWJ80_002013"
     CDS             11511..12257
                     /locus_tag="IWJ80_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9461722.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12254..13093
                     /locus_tag="IWJ80_002014"
     CDS             12254..13093
                     /locus_tag="IWJ80_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9461723.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13094..14044
                     /locus_tag="IWJ80_002015"
     CDS             13094..14044
                     /locus_tag="IWJ80_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9461724.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14185..14937
                     /locus_tag="IWJ80_002016"
     CDS             14185..14937
                     /locus_tag="IWJ80_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461725.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14956..16107
                     /gene="queG"
                     /locus_tag="IWJ80_002017"
     CDS             14956..16107
                     /gene="queG"
                     /locus_tag="IWJ80_002017"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP9461726.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5270 a         3022 c         3254 g         4669 t
ORIGIN      
        1 tttttttggc ttattaaaac aagagatttt tcacgggaaa gtttataact gtttcgagga
       61 attaaaatca gccatcgata gctatatcta ttactacaat aatgaacgga taaaacagaa
      121 attaaactgg caaagtctcg tccaatttcg taaaacaacc gtaacggttg tgtaaaaata
      181 tcattttaga gcataaaaaa aaggagtgga tttctccact ccaaaaaagt ctaacttttg
      241 ggggtcacta cagtgtcaca gtcctttttt tagtaaaagg atacatttct tccttgtata
      301 ttccatgcgt gttcgtgaga aagcatatat aatttaatta acagatagtt agggggagaa
      361 aaaatgattt tttcaattac aatgaaactg gtgttcgggt tgatcggttt attattagtg
      421 gtacgtttgc ttgggaagaa atccatgtca gaaatcacac cgtttgattt agtctatacg
      481 cttgttttag gtgggatctt agaagaatca acctatgatg acaatgtcca tgtagggcat
      541 gtcctatttg ccattgcgct ttgggcagta atgatttatg gtatcgaacg tatcgtccaa
      601 aaaaatgaaa aggtcaatcg ttgggtcaaa ggcgagccat ctgttctaat caaagacggc
      661 gtcatcaaca tgacagagtt gacaaataac catatcgaga tggagcagtt gcgagcaatc
      721 ttaagacagc aagaatgctt ttttcttgaa aatgccaaac atgttatcct agagaatgcc
      781 ggtcaaatga gtgtattgaa aaaatcagat gaagacaaag ctttatcgat cctgcttgta
      841 gatgaagggc aaattcagca taaagtgctt cagtccaatc aattgactga agcatggctt
      901 atggagaact tgaagaaaga aggctatgca gatgtaaaac agctgattta tgttgaatgg
      961 tcagaagaaa aagggctcta catactaaat aaagaagacg tcatgcatga atcttaccga
     1021 atagatggat agatagacag aggttgtgaa atcagcgcct tggcttgtta ctcaaaacta
     1081 agcgactttt tcacaacctc tgatatctaa tatacgttgt tccagaagtt gcttcttcga
     1141 aaaataagcc aaaattccac aaaaatttaa gaagcaattt tcgaaaaatc ctttcttatt
     1201 ttttgaagtt gatcacttct gtcacaacct ctcatgtcac aaaccatttt tttgtttcct
     1261 ttaactcaat ataccgtacg tcttcccaca atatcggctg atggtcaatc ataaagtaat
     1321 cttcataggc ttcaccaatg aagtgccctt cgacctcatc caagagtctt ccaaattcat
     1381 cttttaaatt gagctgaatg attactgctt tacgagataa aaaggcttct gtcagtatac
     1441 tatcgatttc ttgcaatgtc atttgttgtt taggaagagg ctgtttgcgt gcttcttctt
     1501 catttttttt gataccgttg actagttctt ccatcgcaaa agcagtcccc cattttaatc
     1561 caaatggccg atctcgatac tcgttatagt caatgaattt ttttgctgtc cgtctcatct
     1621 tttccacctt ctaatccact cataccacct gcatgcccgc caacgagaga actacgtgcg
     1681 atggcgcgtc ctccttgcaa gagactatgc gcatggatga ttgattgaaa gccgtatttt
     1741 tttctaatcg tatcgacaac aaaatcaact ttcgcttcat ttatctgctt ttcaggatct
     1801 tcaaaaaggt taagctgtaa acctgtcgcg tatacgaggt cggaacagtt cacgcctaca
     1861 cgccgaatat cctggtagtg gtaatgctgg tggaaaatgg tcaataaagc atctgctaga
     1921 tctctgctag catttgtcgc ttcgattttc atttgctggt gaaacccggt cttgccattt
     1981 ggatcaagat agcctaaaga gtatcctacc cataagctga ctacttgtgt ttttgctgca
     2041 gatcgtctca agcgagtgcc gacttggtcg gccatttctt tgataacgat ctcgatttct
     2101 tctcgtcttg tataatctct atctaggatt tgactatttc cgatactttt tgattttggt
     2161 gtatattctt gtcccaaaaa acttcgatcg atgccccaag catgggcata aagctgagta
     2221 ccgatgaccc ctagcttgct tttcaagtga taataatccg cgtgagctaa atcataaaca
     2281 gaatgtatac ccatttgttt cagacgtttt gctgttcgtt tgccgattcc ccaaaagtca
     2341 gtgatatctg agattttcca taacttttct ggtacatctt cataacgcca ttcagcaaac
     2401 atttgctttc cgtcttttga gccattgtct aaggctagtt tagcaagtag cgggttatcg
     2461 ccaattccta tagtcgtgta gattccggtc tgctcaaaca catctttatg gatcagttta
     2521 gccatgtcgt acgcatttgt tttaccaaag agtgccatgc tatttgttac atcaaggaag
     2581 ctttcatcga cggaatagac atgatggttc gcttcgtctg catatttttt atagatagaa
     2641 ttgatttctc tgttcttttc catataatag gccatccttg gggcaacaat atataaatca
     2701 cttggatagg ggaagggaag atctctggca cggctgatat tggtgatgcc gtaagctttt
     2761 tttgctgcag gactggaagc caagattaat ccgctacccc gtgtttctgt cgaatcggac
     2821 ggataggaca tgactactaa tttagttgta aggggatcaa gatccttttc aacgcactct
     2881 acggaggcgt aaaaagattt gcagtcaata cataaaatat ctcttgaagg ttctttttca
     2941 taaacaaata ccggattttg attcgcccac ataacaatca cctcttcaac acaattagaa
     3001 cacacgttct ctttccgtgt caataaaaaa cagcaatttt caaatacgag caaaaggctc
     3061 acatttggaa attgctgaga ggttattttt atctatttgt ttttagctga gatcgttgat
     3121 ttttttatca aacatgaaca gcttctggat cgatctttgt tgtcccattt ccaatgtgtg
     3181 caacataaaa ttccggagca tagccgacaa ctttcatgta ttcatcatag acattgtttt
     3241 tgaaggcagg tatattttct tctttgacaa gagcaatcgc acagccacca aatccagctc
     3301 ctgtcattcg tgcacctaag acaccttctt gtttttgagc agcatctact agtgtatcta
     3361 attcgatccc agttacttca taatcatagc gaagagagtg gtgagaatca tttaacagtt
     3421 gaccaaatgc ttctaagttc ccagcttcta attcagcttt tgccttcaaa gtccgctgat
     3481 tttcagaaac ggcatggcga gctctgcgta tcaatgtatc atcgccgatt tggtcaatag
     3541 ttgattcaaa ttcttcttca tctaagtctc ctaaagaaga gataggcaaa gttttttgca
     3601 aacgtgccaa ggcttcttcg cattcgctac ggcgttcatt gtattttgaa tctgccaatt
     3661 cccgccgctt attcgtattc atgatgacga ttgcataacc atctagtttc actggaacca
     3721 tttcgtattt cagcgtattt gtatctaata agattgcatg atctagctca ccgaagccga
     3781 tggcaaactg atccatgata cctgaattga caccgatgaa ttcattttct acttttttac
     3841 ctgtttggac tagttccaag cgatctgttt ttaaatcaaa cagatcttct aatacaacac
     3901 ctactaataa ttctaatgaa gcgctactgg aaagtcctgc accattaggg attgttcctt
     3961 ccactaataa ctcaaaccca tgatcgattg agtgtccagc ttcttttaat ttaaggacca
     4021 ttccttttac gtaatttgcc cagtttgctt ttttatcata ttctagctca tctaaagtaa
     4081 aactaatgac accgatatcg tcaaagtttg tggaataaac taatatttta ttatcgtctc
     4141 gaggtgctgc tacaccttgc gttccataag tgatggaagc ggggaagaca tggcctccat
     4201 tgtaatctgt gtgttcacca atcaaattga tgcgtcctgg tgagaagtat tgtgtagttt
     4261 ttttaggtcc gaatgtttcg gcaaattttg tgtttaagct tcttttgatt tccatgatga
     4321 gttattcctt tcttgttttg attttgaatt ctgtcactgt ttcaaatact gatccggctt
     4381 ttaaatgaat cgaaccaaat gctgagaagc gctctgcacc tggagctgtt tgagtctcaa
     4441 atgtgatgcc accgtgatgt gccagttttc ggttgcgcat ttctggtgtt tctgtgccga
     4501 aatttgcagt gaaaataacc acactagaag catctgtagc aatatctact tggattcttt
     4561 tatcggggct agtcaagcga gcggcttctt tacccaagcc ggtttctttt aagaaaaatg
     4621 gatgatcgat tccgtcaaca agattttttt gatccaaatc actagcaaaa acggacgcta
     4681 attttttagg tatttgaaaa tcgaaagcac ttcctgcagc attttctttt acccctatag
     4741 gaatactatc tgttcgcaaa ggagcataag cttcgctatt gagccaaagc tcatgttgat
     4801 cgattgactg agaagcatct cccgtcaagt tgaagtaaac atgatttgtc ggattgaata
     4861 aagtatcttg gtcactggtg ccgcgagagg tcactcgcca gatattatct tttgtcaaag
     4921 tatagcggat ttccacatcc attgtacctg gaaaaccatg ttctccgtcg ggacttgtag
     4981 tagtaaaaat gacagaagct tcattttcgc cattcaaaat gacataagac cattttttta
     5041 attcaaatcc aggagcacca ccatgcagat tatgtccagt ttttggatct gtggctaatt
     5101 ggtaagtttt tccattgaga gaaaagtggc cattttcgat tctccctgcg gtgcgaccga
     5161 tagaagcacc gatatacgga tctttttcga tatattcttc agcggtgtca aatcccaaaa
     5221 ctaattctcg atcatttgat tttaaactga cgattctagc acctaaatca gagatagaaa
     5281 gtgctagctt gtttttgttc tccaaggtta tcaagtgata accttggcca aaatcttttt
     5341 ctgtaatctt cattttcaag atcctttctt aatgggaagg aactactgca tcgtgaagtt
     5401 catcttccat tgtatcagct ggagccaatt tgttttccaa tttctcaacg atttgtgcat
     5461 gttttttctc tgtcaatgta atcttccaag caaaaataat agcggagaaa atcatcaaag
     5521 cagcaggagc atagaatgca taagctttga aagtcgttac gccatgagca gtgatatcag
     5581 cagcagtagc attaccagtc atcccagaag cgctagcgac aaaccctacg atcccgttag
     5641 acaaagcacc agcgatttta tctagtaaag ggcgaattga tagagtaaca gcttcgttac
     5701 ggataccatt tttccattga ccatattcaa cagagtctgt gatcgtcatc aaagcggata
     5761 agaaaatcaa ttgttgaggg aagtagaaca atactagccc gatgataacg attggcaaac
     5821 ttgaacctgc gatcgtaaag aaaaagtaag aagaaatcat cattgcgatc cctccaaggt
     5881 aaacgtagcg acgagagatg acgcgtgtaa gcattgggaa tagtggaaca gctactaaac
     5941 caagtaaggc agcaactaga ccaacgatcc aaaattcagt tggttggcca agtacatatt
     6001 tgaagaagta gaataatacg gcagttgttg ctacattggc aatcgcataa gcgatataag
     6061 caagggcaag ccacatcaac tgatcatttt gagcaactgc tttgaacaca tctttgatcc
     6121 cagctttttc attttgctga cggatcgcag tatcgacttc ttttgtgccc aaggcagtga
     6181 taatcgcagt accgcctgaa ataacagcag caatcacacc gaaccagaac cagcctgaag
     6241 cgccttcaat atgtttatga gtaacaagat aggtaaagaa agtaacgata gggacaacga
     6301 ctagagtagt accatttgca cctagagaag agccgaaacg agcaaacgta gcaaattttt
     6361 cacgttcctt tgtttccgta ctcaaagttg gaatcattga ccaaaaagcg atatccttaa
     6421 atgaataaaa actatccaat aagatgaaaa cgattgcaaa taatatgata aataaagtcg
     6481 tattatgagt agctaagcca aagaaattgg taaagattgc taccaagagt acggaactaa
     6541 caacgccgcc gataactaac catggtttga atttaccgta ttttgtacga gtattatcga
     6601 tgatgccacc aatcaaagga tcaaaaataa tttcaactaa gcggatacct acaactaagc
     6661 ttgtaactgt agcaatcatt tttgcattcg ttgccttatc tgttcctgcg aacatctggc
     6721 ttgtcacgaa taccataaaa taagtgctta atgtaacata gaatgtgtcg tgtccaaaag
     6781 cgcctaaagc gtaggacaca cgttggatca ttttgctttt cataatctgt attcctctcc
     6841 tgttcaatat ataatactaa gtaaaccggt tacaacgaat aacaaagatc ttttaatgac
     6901 ttaaacttaa caagaaatcg cttccttttt aatatatttc tgtgactttt atttaacttt
     6961 ttgttgcatt atactaaagg agtaaggagg gatagacgtg gagcaatcga tttttttaaa
     7021 aaaggaacat caacaagtag aaaaagaatt gtattttgat ttttgtggtt tctctaaaac
     7081 gatgccgttt cattcttttg gtcctgctat tcgcaatgat tacatcctgc atgtcgtgat
     7141 ggaaggcaaa ggatcttatc atgtgaaaaa tcaacaattc caattgaaaa aaggtgatct
     7201 gttcttgatt cgtccaggag actcgacgtt ttacttatca gatggagaag atccgtggat
     7261 gtattgctgg ctttcatttg gtgggagtgc tgcagaagaa atcattcggc agtcgctttt
     7321 cagagaagac caatacacga tggtctcttc tgacatacaa aaatatatcg acattatttt
     7381 agaatgcatg aaagatacgg aagaaacctt ggaaaatgaa ttacagttaa cagcattaac
     7441 ctaccgtttt ttatcagtcc ttttaaaaga tggcggaaag gtcagcggtg gtgtacaaaa
     7501 ggttcattcc tctttagctg ttgaaacagt caaatacatc gaggcacatt atgctgaaaa
     7561 attgtctgtt gaggaaatcg cccagcattt agcggtcaat cggagccatt tgtcgcgagt
     7621 attcaaaaat catatgggta ccagtatcaa ggaatatttg atcggggtac ggatcaatcg
     7681 aggggcgttt cttctttctc tgacgggtga atccgtggaa agtattgcct atcaagtagg
     7741 gttcaatagt ctagtcgtat ttagccgaat gttcaaaaag agtaccggag aaacggctac
     7801 gaactatcgt aaacgtatga aaaacgaaaa ttttaagaat attgctttgg ataccttgaa
     7861 aaaacagttg gaaaaacaaa ccgcagtttc ttggacaacc tgattagtct ggttaaaatg
     7921 acaagcatcg gcaaaagaaa cttgtggcgg ttatgctaca ctgtaaagcg aagactaaaa
     7981 aggaggaaaa gggatgcgta cacttatcat cattagccat ccaacgatcg aagaatcttt
     8041 aaatcagcaa tttttcaaag aagcttctgc tggtttacca gctacttggc atcatttgga
     8101 atcaagttat ccagacggaa aaatagatat agagactgaa ctacagctct tggcccagca
     8161 tgatcggatc attttccagt ttccgtttta ctggtatagt tctcctgctc atttaaaaat
     8221 ttggcaagat aatgtcttgg aacgagcagc acatgtatta aaagggaaag aactaggaat
     8281 cgtattgaca acaggtgtgg cggaaaaaga ataccaagct ggaggaaaag aagaatatac
     8341 gatttccgaa tttttgaggc cgtatcagcg aatcgcaaat aaattccata tgacctatct
     8401 tccgccattc gttttagcac aatttatgta tttatcccag gaaaaacgtt gggagaagct
     8461 aattgcttat cagcagtatc tctcattgga aggcaaacca tcgctgactc agcggattga
     8521 ctggtttatt caaagagtac aggaaaatca gaaaatgcag gaagaagaat ctgaaaaaca
     8581 agcatatatc gtagaagcac tgactgaggc aaaagagcag atcgaagact tgacctttac
     8641 actgcaagaa atgaaaggaa cttcattatg acaaatgatt ggctgatcca acaattagat
     8701 gaattaagcg aaaaatcaga atatcaagac cgtgcgctct tatatgaaat aaagaaactg
     8761 atggctgaga tagacgaacg aacaatgcag ctgcaaggag aaatcgacgg acaatcttgg
     8821 aatcatctca actggtgaaa ttttcttata aataacaaag ataaaaatgt gcacagcgac
     8881 aattatttgc tacaataaga atatgaagcg tttgataacg ctattataca taaaaattgg
     8941 tggtgaaaaa aatgtatgat gaaaatgagt ggggagaaaa tgttcggatg acccgaagaa
     9001 gacagcaaaa acaggcagct cgtaagcgga tgatcatgat tggggcagca gttgccgtgt
     9061 ttttgatcat agcaggagga gttgcgggtc tatattttca tggaaaaaat tcgtcagaag
     9121 cgaaaagtac gaccataacg gaaacaacga caaagaaaac agagacgaaa caaacaagtg
     9181 agtcatcaac taaaacaaaa gagacacaaa cttctgaaga aaaaacagca aatggtttgc
     9241 cagagcaact tcaagcaatc gtaaatgatt attcgaaaaa gatcgaagaa aagacgcctg
     9301 cattgattga agaataccag acagaaattc agggaaatca agagggaata gctggtttat
     9361 cagcagttgc taaccaaaaa gcacgtgagc ttcaagcaat ctccgacgaa gggatcagaa
     9421 aattacgtgc agcgtatcag gcagcagaaa ataaagacgg tgtagactta gatactttga
     9481 tcaatcaatt atcggctaat tatacgaatc atgtcgctaa gatttccgat atttatttgc
     9541 agactagcgc ctctcttcaa gcagagtcaa caagcacaca agacacaaca tcttctgatt
     9601 ctgagacagc agagtcggta gaagcttctg aaatgcaagc tcgaacaaca caagacacaa
     9661 cagattcctc tgaaactaat accaacagcg cgacttcaca ggctgcgaca accgtagttt
     9721 tggaaggaga aggtccaaat caaatagccg aacgaactgg cgtaccagta gaaacgatct
     9781 tgtcgctgaa tgggatgagc atggagaatt atttcctgac tcccggtgag acgctaaaat
     9841 tgaattgata aaagcaataa aaaagaacga agcttccact aaggtgaagc ttcgttcttt
     9901 ttttattttc cggttctttt ttcgcgtaca aagtaatgga tcgtcatgta aagcaagaaa
     9961 acgagtccaa taatcaatga gattctcgtt tcaggattga taaacatgaa aatcaatacg
    10021 atagctaaaa aagcaagtgt cacataattc gtataaggag atagtggcat tttaaaagga
    10081 tgatcatgca ttttatctgc gtgcttcttt ttgaagcgaa tctcgctgat caaaatcact
    10141 acccaaggaa ccataccagg caaaatactt gagctgtata cgaaaacgaa aatattttct
    10201 gactggttga tgaacattgg tagaagataa tttaagatca gaccaattaa gattccacct
    10261 gagattgcta cgacaggata aaaaggcgca ccgtgacgag atagtttcag aaatttttta
    10321 gacatctttc catttatggc aagtgtatag accatacgac ttgcgctgaa aatccctgaa
    10381 ttgcaacctg ataatgccgc tgtaatcacg acaaaattaa tcagtcctgc agcaaatgta
    10441 atgccgactt tagaaaaagt ttgtacaaaa ggtgaaccaa tctcactcaa ttgattccac
    10501 ggatagatac tcacaatgat aaaaatagca ccaatataaa aaatcaagat tcgaccgata
    10561 gtggactgga cagcttcaat gatcgtcttt ttaggattct ctgcttcacc agcagtcatt
    10621 ccgatcagtt cgattccttg ataggaagca acaacgatcg ataatgcaaa gaaaaatcct
    10681 ttgacgcctc cagtgaagaa tccaccattt ttccaaagat ttgaaatccc tactgcctga
    10741 ccatgattac caaatccaaa taagattacc ccaagacctg caatgatcat caaaatgatc
    10801 gttatgactt tgatgatcga aaaccaaaat tctaattcgc caaacgcctt gacagaaata
    10861 agatttgcca gcataagaaa caagacgaca atgattccgg gaataattgc cggcaaatca
    10921 ggccaccaat aattcatata agatcctaca gcaatcactt cacttattcc tacgacaata
    10981 tattgaaaaa tattactcca agcggtcaag tagccagcca caggatggat gtactcgctc
    11041 gcgtaatttg caaaagaacc agtcgaagga tcaacgtaaa gcatttcgcc taaagcacgc
    11101 ataatcaaat acaaaaagaa tcctgcaatc gcataggctc ccatcactga gggccctgtc
    11161 caacgtatgg ttgaggaggc acccataaac agaccgacac cgatcgttcc acctagtgcg
    11221 atcatttgga tatgacgggc actaagactt cgagttaact tttcttcttc catacaaaaa
    11281 ctcctttcgt tgttgagtca acaagaaaaa ataatcaaac gtttcaacac atgtttttca
    11341 ttattaggtt agccttattg aactcttgat gtgattctac agaaaatttt cataaaatgc
    11401 gaggaaaatt cgaaaaaaac tttttcatca ttctacttga aaatgctttc cattttagtt
    11461 aaactatatt taaataaaaa gttaggtaaa cctaaaaagg agtgtaagat ttggaacgta
    11521 cggccattgt aattcaagac ctttcagccg cttatcaagg gaaaactgtt ttgaaaacta
    11581 tcaacttgac gataaatcca caaaaaatta ccggtattat cggaccaaac ggtgcaggaa
    11641 aatcaacatt tatgaaatct ttgctagagt tgataccagc tactactggt aaagtcacct
    11701 tttctggaca gtcggtaaac tcagtccgta aaaaaatagc ctacgtggaa cagcgaagtg
    11761 aattggatct ttcctttcca attgatgtat taggcgttgt acttttagga acatatccat
    11821 ctttacgaat tggacaaaga cctgggaaaa aagaaaaaga acgtgcaaga caagctttga
    11881 aaaaagtagg gatggaagaa tatgccaaaa gacagatcag cgaactatcg ggtggacagc
    11941 ttcagagagt ttttattgca agagctctag cccaaggagc agaatggatc tttttagatg
    12001 aaccattcgt agggattgat gcgttaagtg aacgaaagat ctttgacatc ttgcaggaat
    12061 tgaagaattc aggaaaaacg attttgatcg tccatcatga tcttcataaa gtagaagaat
    12121 atttcgatga ggttattctt ttaaataaac agctgatcgc ttccggtcca gtacaagaag
    12181 tctttacatc gaagaacctt caattggcct atggtgagat catcagacat ttagtaaaag
    12241 gaggagagaa aaaatgatcc aatcctttat tgatggatta atgaattatc aattcctgca
    12301 atacgctttg gtcacttcca tgcttgtagg acttgcttct ggagtcatcg gttcttttat
    12361 tattttacga gggatgtctt tgatgggaga cgctatctct cacgcagttc ttccaggtgt
    12421 agccatttct tatatgtttg gttttagtta catctttgga gccactgctt ttgggatgct
    12481 aacagcagca gtgattggtt tcgtgactca gcggagccga ttgaaaaatg atacagcgat
    12541 tggaattgtc tttagctcct tttttgcatt agggattatc ttgatttcat atgcccagag
    12601 tgcgacagat ttgtatcata ttttatttgg gaatgtctta gctgttaggg aaagcgattt
    12661 gttgctgaca gcactcgtta gtggaatcgt cttgatattt gtctttttct tttataaaga
    12721 attgaagatc acatcattcg atccgacaat ggcaaaagct tatgggctga acacatcgct
    12781 gatccattat cttttgatgt tctttttgac attagtggct gtagtcagtt tacagacagt
    12841 aggaacaatc ttggtgattg ccatgttgat cacaccagcc gccacggctt acttgctaac
    12901 gaaccattta ctgaaaatga tcattacagc tgcaggaatc ggtatgctaa gtgcagttgt
    12961 cggtgtgttt ttcagttata gttacaattg gccatcagga gctacgatcg tgttagcatg
    13021 taccgcattt tttatccttg cttttttatt ttctccaaca aaaggaattt tatttcagaa
    13081 aggaagcaga tagatgaaaa caaaaaaatc actcttttta atcttggcag ttagtttctt
    13141 ggtattagcc ggttgcggaa agcaggcttc tgaccaagcg gatgaaggaa gtaaagaaaa
    13201 gttgtcagtc gtggctacca attcgatctt ggcggacatg gcaaaagagg tcggaacaga
    13261 tcaaatagat atccacagta tcgtaccagt cggaacagat ccgcatgaat atgaagtact
    13321 accagaagac atcaaaaagg caagcgatgc agatgttatt ttatacaacg gtttgaatct
    13381 tgaaacaggt aacagctggt tcgataactt gatggaaacg gctaaaaaag aagaaggaaa
    13441 agattatttt gcagttagca aaaatgtaga acctctttat ttaactagcg gtgaagaaca
    13501 tacaaaagca gatccccatg cttggctaga cctatctaac ggaataaaat atgtggagga
    13561 aatcgcacgt atattctctg aaaaagatgc agaaaatgcg acactctata aaaaaaatgc
    13621 agaagcatat gtggaaaaac taaaagaatt agatacacaa gcgaaggaaa gttttgcttc
    13681 tatcgaagag aacaaaaaat tattagtaac aagtgaaggg gcattcaagt atttttcacg
    13741 agcatatgat ttgccagcag cttatatatg ggagatcaat acagaaagtc aaggtacgcc
    13801 tgatcaaatg aaagcgatca ttgatcagat aagagcaaaa gaagtaccgg ttttattcgt
    13861 ggaaaccagt gtagactcaa gaagcatgga acgggtagca aaagaaacgg gtttgaaaat
    13921 ctatgataaa ctgttcactg attccatagc aaaagaagga gaacaaggag attcttacta
    13981 tcagatgatg aaatggaata ttgaaacgat ccatgaaggt ttgtcacaaa caaaagaaag
    14041 ctgaaaagtg caaggtctag cttgaggagc agaccttgca ttttttataa gagatatgaa
    14101 aaaaaattat gctgtttttc gtcgtacaga tacctttttt gaagaaattc cgttatagtt
    14161 agagtaagga aggaaggtgt ggctatgaat tttacacgta aagataaata tacgattaaa
    14221 gaagaagtct ttcgattgat cagccagcag gaaaataccg acaaggatca agaccttcaa
    14281 tttcttgaag cagaattagc taaattattc atagcaaatc aaaatgagac aacagaacca
    14341 gatgaagagc tgtacatagg caaatggttg tttgatgaat ttcagccttt agatgaaaat
    14401 accggtatcg gttattttgt gttcctttct ccagtcggct tcacacctgg cgtattagtt
    14461 aaacaaaacg acgaaataac cgtcaaagca tggcctgcat tattttcagt catcaaagca
    14521 gaaggagtaa tcaaaaagat ccagtcggcc tattataaaa gtcaatttat tgccaacagc
    14581 aaattaatga atatgtttta taatcgtcta gtagaaattg ctaggcctgg gaaaatcccg
    14641 caaacattca cgtacacaca gcaaaaaaat ctctatgaag aagtattgta ttcccgcgat
    14701 ctgtctgagt tttcgccatg ggaagaggta aggctccatg aactgttaag tacaaaacca
    14761 gtttcattga atttttttaa tgcattagat aacaaaagaa aattaatttc aacaaattat
    14821 tgggaagtat ttgctgcaag aaaaaaatgg atggaagaaa agaagattgc cgatcagcag
    14881 atccagtttg tccagctgaa aaaagaaaaa ctatattatg ggttatacag ccgttaacag
    14941 caagcgagga agaccatgac attaaaagaa gaaatcatac aagaaagcaa acgattagga
    15001 attgacaaaa tcgggttcac cacggcggaa ccttttgatt cattaaaaga atcgttggaa
    15061 gaacaaaaag cagctggcca tacttctggc tttgaacatc caaatatcga tgagcgtctt
    15121 tatccagaaa tgacatttga tcagccgaaa tcgatcattt ccattgcact tgcgtatcct
    15181 actaggatcc atgaagaagt gcctagggat gaaaaacgcg ggcagtttgc tcgtgcttct
    15241 tgggggatcg attaccatga tatactcaga gaccgtttga accgattgat cgaattcatc
    15301 caaacaagag cagaaaaatg gcaaaaagaa gaggaatggc gtctagctcc gcaagtagat
    15361 actggagaac tgatcgatgt cgctgtagca cagcgagctg gtttaggatt tattggacga
    15421 aacggattgt tgatcactga agagttcggt tcatttgtct atcttggtga gatcgttact
    15481 aatattgtat ttgagcctga taaaccagga gaatttggtt gcggtgactg tatgcgctgc
    15541 gtgactgctt gtccgacaaa agcattgctt ggtgacggaa gaatgaatgc ccagcgatgt
    15601 ttgtcttatc aaactcagac aaaagggatg atgccggaag aatatcgtaa aaaaatgcga
    15661 aatgtcattt atggatgtga tatctgccag ttagtctgcc cttacaatca aggaaaagat
    15721 tttcattttc atgaagagat ggaaccaaaa gtagacgaaa tatatccaaa attaaagcca
    15781 atgctttcga tgtctaataa agacttcaaa cagcaatttg ggcatttagc tggttcatgg
    15841 cgtgggaaga aacctttgca gagaaacgca ttgattgctt tagctaatct aggtgatcga
    15901 acggcattgc ctgagatttc agaatgttta acagatgtcc gccctgtcat cagaggaaca
    15961 gcagcttggg ctattggacg tctaggaaca aaagaaccgg aaaaatggct tgatgtatta
    16021 gatgaaatat tagctaaaga aacagaagaa gtcgttataa acgaattgga acatgcccag
    16081 aaattattgc ggagaaagtt aaaataaaaa aatagttaac attattcaaa gtttttgtcc
    16141 agtaattgtt gtaacaatta ctggtttttt tgtatttttt acattttttt aagagaattt
    16201 gataaaaatc aaaaa
//
LOCUS       DACDRN010000054        15315 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDRN010000054 DACDRN010000000
VERSION     DACDRN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.054"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..513
                     /locus_tag="IWJ80_002018"
     CDS             <1..513
                     /locus_tag="IWJ80_002018"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9461727.1"
                     /translation="NVVVYILGHGTKKELLENLIKAYQLQNVIFLLGGQSNPYKYMSR
                     ADLYVMPSITEGFPNALVEAMSTETAVLCADCKSGPREILSNRALDTICENIEYAESG
                     ILVCPMTDSRNYSPQYEECDKNLANAIEELIENDNLRNKYAKAAKKRAELYSYDAFEH
                     NLLQIMKTFN"
     gene            551..1642
                     /locus_tag="IWJ80_002019"
     CDS             551..1642
                     /locus_tag="IWJ80_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9461728.1"
                     /translation="MLKKNTSLQNKTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFLFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
     gene            complement(1710..3188)
                     /locus_tag="IWJ80_002020"
     CDS             complement(1710..3188)
                     /locus_tag="IWJ80_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP9461729.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRVESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(3454..4202)
                     /locus_tag="IWJ80_002021"
                     /pseudo
     CDS             complement(3454..4202)
                     /locus_tag="IWJ80_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            4345..5295
                     /gene="pfkB"
                     /locus_tag="IWJ80_002022"
     CDS             4345..5295
                     /gene="pfkB"
                     /locus_tag="IWJ80_002022"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9461730.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQKKQIQTEFIEVPGMTRINVFTQVNQTREE
                     YKLVNQGPEIPQAAVHNFLNQIRALQAEDYLCVSGSLPRGMSPKVLVEISRICQEKEI
                     HLIIDSSDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENV
                     LLSLGEKGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLVGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            5277..6740
                     /locus_tag="IWJ80_002023"
     CDS             5277..6740
                     /locus_tag="IWJ80_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9461731.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTILADNEAVDTLESETISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMLKTVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     SGGTGFLVGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVVFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPINKAAYVTGTALLAQGN
                     TTFMAGVSVACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEEGRAGGLS"
     gene            6737..7195
                     /locus_tag="IWJ80_002024"
     CDS             6737..7195
                     /locus_tag="IWJ80_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9461732.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKEVYRK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            7192..8166
                     /gene="lacD"
                     /locus_tag="IWJ80_002025"
     CDS             7192..8166
                     /gene="lacD"
                     /locus_tag="IWJ80_002025"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9461733.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPECGLPAAALREKECGLLLAYEKTGYDTKMPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECDAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLKETATPVYVDR"
     gene            complement(8345..9295)
                     /gene="yidC"
                     /locus_tag="IWJ80_002026"
     CDS             complement(8345..9295)
                     /gene="yidC"
                     /locus_tag="IWJ80_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9461734.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(9733..10008)
                     /locus_tag="IWJ80_002027"
     CDS             complement(9733..10008)
                     /locus_tag="IWJ80_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP9461735.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            10116..10880
                     /locus_tag="IWJ80_002028"
     CDS             10116..10880
                     /locus_tag="IWJ80_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP9461736.1"
                     /translation="MKEIQSAKNPKIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            11003..11506
                     /locus_tag="IWJ80_002029"
     CDS             11003..11506
                     /locus_tag="IWJ80_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9461737.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            11914..12561
                     /locus_tag="IWJ80_002030"
     CDS             11914..12561
                     /locus_tag="IWJ80_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP9461738.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALATEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMIYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            12724..13992
                     /locus_tag="IWJ80_002031"
     CDS             12724..13992
                     /locus_tag="IWJ80_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP9461739.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(14019..15218)
                     /locus_tag="IWJ80_002032"
     CDS             complement(14019..15218)
                     /locus_tag="IWJ80_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9461740.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
BASE COUNT         4845 a         2601 c         3163 g         4706 t
ORIGIN      
        1 aatgttgttg tgtacatctt aggacatggg acaaaaaaag aattattaga aaatttaata
       61 aaagcatatc aactacaaaa cgtaatattt ttgctaggtg gccaatcaaa tccttataaa
      121 tacatgtcac gtgcagattt atatgtcatg ccatctataa ctgaaggttt tcctaatgca
      181 ttagttgaag caatgtctac agaaactgct gtcttatgtg cagattgcaa atctgggcca
      241 agagaaatat tatcgaatag agcgctagac acgatttgtg aaaatataga atatgctgaa
      301 tcaggcatat tagtatgtcc aatgacagac agtagaaatt attcgcccca atatgaagaa
      361 tgtgataaaa atttagcgaa tgctatagaa gaattgattg aaaacgataa tttgaggaat
      421 aagtatgcaa aagcagcaaa aaagagagca gaactatata gttatgatgc ctttgaacat
      481 aacttgttgc aaattatgaa gacatttaat taattcatat gatgaaataa aaataaggga
      541 gtataactag atgctgaaaa aaaatacatc tttacagaat aaaacaatat taataactgg
      601 cgcagccggt tttattggat caaatttggt tcttgagtta ttacgtactc agtcatcaat
      661 aaaaatcatt ggcattgaca atatgaatga ttactatgat gtctctatta agaactatag
      721 attatcagag atagaaacgg aggcaaagaa ccatccagga tctacttgga attttatcaa
      781 aggttctatt gctgataaag caacaattga tttcctcttt gataattata aaccaagtat
      841 cgtggtgaat ttagcagctc aggcaggagt ccgttattca attactaatc cagatatgta
      901 tatcgaatct aacataatag gattctataa tattcttgaa gcttgtcgtc attcatatga
      961 taatggagaa tctggtgtgg aacatttagt gtatgcctca tcgtcttccg tttatggtac
     1021 taataagaaa attccttatt ctacagagga taaagtagac aatcaagtat cgttgtatgc
     1081 agcaactaag aaaagtaatg aatcaatggc acatgcatat tctaaacttt acaatattcc
     1141 gtctacaggg ctacgtttct ttacagtata tggtcctgct ggtcgtccag atatggctta
     1201 ttttggtttt actgataaac ttcgtgctgg tgataatatc caaatattca attatgggaa
     1261 ttgcaagcgt gactttacct atatagatga tattgttgaa ggagtaaaga gggttatgca
     1321 gtgcgctcca gagaaaattg atggggaaga tgggctacca atcccaccat atcatattta
     1381 taatattggt aacaatacgc cggaaaacct actagatttt gtagatattc tacaacaaga
     1441 gctaattgca gcaggggtgc taccagaaga ttacgacttt gaatctcata ccgaactagt
     1501 tccaatgcaa ccaggagatg tgccagttac ttatgcagat actagtgcgt tagaacgaga
     1561 ttttgaattt aaaccaagta caagtttgcg tgacgggctt cgtaaatttg cgcagtggta
     1621 taagtctttt tatgaagtat aattgtattt ttttacttat aaaagtttat agaaacacct
     1681 cagacactac aaatctgagg tgttattttt taatctcgtt gaaataaatc tctggtatat
     1741 actttttctt ttacatcatc aagattacta tcgtaacgat tagcaataat actgtgactg
     1801 atatttttaa aatcatctaa attatttatt acttttgaac caaaaaatgt agtgttattt
     1861 tcaagtgttg gttcataaat tacaatagtt gccccctttg ctttaattcg cttcataact
     1921 ccttgaatag atgactgacg gaagttgtct gaattactct tcattgtaag acggtataca
     1981 cctatgacta cctctttttc tttttctgca ttccacgaac tgttagcctc atacgtgcca
     2041 gcaagttcta acactcgatc tgctataaag tcttttcttg tacgattact atcgacaatt
     2101 gcacttatca tattctgagg aacatcgtta tagttagtga gaagttgttt tgtatctttc
     2161 ggtaaacaat agcctccata tccaaaagat ggattattat aatgtgtgcc aattcttgga
     2221 tctaagcaaa ccccgtgaat gatttcttgt gtatttagtc ctttcatttc tgcataagta
     2281 tccagctcat tgaagtaaga tactcgaagt gcaaggtaag tattagcgaa taatttaact
     2341 gcttcagctt ctgtgaatcc catgaaaaga gtttcgatat cttttttgat tgctccttct
     2401 tggagtaaat ctgcaaaagt gtgagaggct tctcgtaaag ttttgtcagt ttggtccgtg
     2461 ccgacaataa ttcgcgaagg atatagattg tcatataatg ccttagattc tctgagaaat
     2521 tctgggctga atagaatatt tttagtattg tattttttac gaatactttc ggtatagcct
     2581 acgggtaccg tcgacttgat gaccattatt gcctttggat tgacttctaa caccaatttg
     2641 attactgact ctacagcaga agtatcaaaa taattttgtt tactatcata atttgttgga
     2701 gcagcaatga caacaaaatc ggcttctcta taggcggctg caccatcagt cgttgcaatt
     2761 aaattaagat ttttttcagc tagatatttt tcaatgtaat cgtcttgtat tggagatttc
     2821 ttattgttaa tttgttttac tttttcagga agaatgtcta ctgctgttac ctgattgtgc
     2881 tgggcaagaa gagtagcaat ggaaagacct acgtaaccag taccggctac agcaatattc
     2941 ttcgactgat gttttgttct gtttgattta tttgattcat ttataaatag atctgttggt
     3001 tcaaaattta gaacttctgc taatgattct aattgttgta ttgacgggat aaaatcgaga
     3061 gattctactc gactaattaa ggcacggttg ataccagtag ctactgcaag ttgttgttgt
     3121 gtcattttca gagattttct agaatcaata atgatttgag ctaaacgttt ttgagataac
     3181 tttttcatga tgcactcctt tttttgttgc taataataac aaacaatttt ttcttctata
     3241 acagaatagc ttattattaa taaaactgtc aattttttta ttgattaaaa agatattttt
     3301 tgattttaag tattttcaat tattttttgt tacttaaaat aactttatag attgtgaata
     3361 actttataca taaaaagttt acgtgagtcc atacaatata aagaataaaa aatgtgatgt
     3421 tttaaactgt aaccaaccat aataaactgc ttctcaattc tccctcatac gcaatacata
     3481 ggcgtcaagc aggattctcg cgatgatctg gagaggcaag cgtgaacgta cttcataatc
     3541 agggaaataa gaaccttctg atttgaaacc tacgagattt atctcgcaat aatctttgag
     3601 tgtagaggaa tgattggctg taactagaat cgaaccaatc tctgcatcat agcagttctt
     3661 tgcggcggtg acgagttctt ttgtctcgcc gttcaaagaa acaaaaataa cgctgtcttt
     3721 cttagttagt ttccgactga tgcttttgat aatctctgga tcagtatgca tttgacaatt
     3781 ttttcctgtt aattgaagtt ttacgagcat ctcttgacca atcatttctg aaaatcctcg
     3841 ggcaaagaca aaaacccggt cagtaaattt gattctttgt attgcatctt caatgttccc
     3901 gatttccaac atattgatcg tacgtaccac ttcttgttca ttttttagaa tggctcgacg
     3961 gatacctttg tctactttat ctaaaaagaa aaattgatgt tcttgttttc tccttctttt
     4021 agatggtgct taaacgaagt aaatccttca tagccttttt tcttcattgt ccgcacaatg
     4081 gtggctgttg agacatttgc ttgttcactt agtttcacga tggagtagtt agggatctct
     4141 aggatgtggt gttcgataaa gttccagagg tactgttctg tttcacttaa tttgtgatcc
     4201 atattctttc cttctttcac taaaagttag ttaaaaaata gcacgatgtg tttataaatg
     4261 taaaggtttt ctttgtgttg gaacgattaa gaaaacattt tctctttcgc gtatttcata
     4321 atagattcaa ggaggtcatt ggttatgtct atctacacat gcacgatgaa tttggcaatt
     4381 gatctattta ttgaaacaga ggagatgcat ccttttatgg taaaccgtac aaaggaggat
     4441 gacattcagg caaacggaaa aggagtaaat gtgtcactag tattaaagat gctagatttc
     4501 ccaagtacgg cattaggttt ttctgctggt ttcacaggaa aatatataga agactattta
     4561 cagaagaaac aaatccaaac agagtttatt gaagtgcctg gtatgacgcg tatcaatgta
     4621 tttacacaag tcaatcaaac gagagaagaa tacaaattag tgaatcaagg acctgaaata
     4681 ccgcaggcag ctgtccataa ttttttaaat caaataagag cattacaagc agaggattat
     4741 ctttgtgttt ctgggagttt accaagagga atgtctccga aggttttagt ggaaatcagt
     4801 cggatttgtc aggaaaagga gattcatttg attattgata gcagtgatca agaggtcatg
     4861 gattgtttgc cctatcgacc atttttgctg aaaccgaatg aggaagaact ttcttcttgg
     4921 ttcggacgaa aaatggttac agatgaagat tactttgttt atggacaacg actggtagag
     4981 tcaggtgcag aaaatgtttt gttatccctt ggtgagaaag gagcgattct ttttacgaaa
     5041 gacagggtga ttcgaggaaa ctcgccaaaa ggaaaagttg tcaatacagc atgtgcaggt
     5101 gatgccatgt taggcacttt tttagtagga tacatgaatc gacgaccgct tgatgaaacg
     5161 ttacgtaaaa gcatagcagc aggcagctct actgcttttc gtaaaggact gactgatttt
     5221 ttggatgtgg aggaattaag taaacaaatc aaaatcaagg aagagaggtt cgaaagatgg
     5281 gaaaatatca actaattgca gcaaccggtt gtccgacagg aattgcacac acctacatgg
     5341 cgcaagaggc attagagcaa gcagctagaa aaaaaggcat cacgatcaaa gtggagaccc
     5401 atggacaaat tgggatcgag aatgaattaa cggctgaaga aatcaaacag gcagatgctg
     5461 tcattattgc cgcagacaaa gatgtccatt cagaaagatt tgcgggaaaa cgagtcattg
     5521 atgtttctgt cagtacagga atcaaggatg cacaacggct gattgacgaa gcattggcag
     5581 gaaaagggac catcttagca gataatgaag ctgttgatac tttagaatca gaaacgataa
     5641 gtggtgctaa ttttggtcgc agcatctata aaaatttaat gaatggcgtc tctcatatgt
     5701 tgcctttcgt ggtagctggc ggagtattga ttgctatttc atttgccgtt tggggaatct
     5761 attcttttga tccagaccat gcacaataca atgctacagc ggcgatgtta aaaacggttg
     5821 gcgatgcagc aatggggatg atggttccag ttttagcagc ttatattgca gaagggatcg
     5881 cgaaacgtcc agggttagtc gttggatttg ttggtggaat cgtggccatg agtggcggta
     5941 ctggattttt agtaggtatt ttagctgggt tcttagcagg ttattttatc ttatttttgc
     6001 aaaagctact gacaatttta cctaaacaac tagatggctt gaaggcaatc ttcttatatc
     6061 cagttattgg tgtagtgttt atcggcatcg tgatgtcttt gctggcgggt ccaatgaagg
     6121 ctataaatga aggaatgatg agctttttag ctggatttga acattcgaat ccacttgttc
     6181 ttggcgtgat tgttggttgc atgtgtgcgt ttgatatggg aggaccgatt aataaggcgg
     6241 catatgtaac aggtacagct cttttggcac aaggaaacac gacttttatg gctggcgtgt
     6301 cagtagcttg cattgcacca ccgttgatta ctggttttgc aactttattg tttggtaaat
     6361 attttgatac taatgatcgt aatgcaggtt tggttaattt tattttagga tcaactcata
     6421 ttacggaggg agctattccg tttgctgcga aagacccgat ccgagtatta ccaacgatga
     6481 tgttaggctc atcaattgct gctgtcttga cctatctgtt tggtgtccaa gtaccagccc
     6541 cacatggcgg ttttttagtg ttacctgtag tgacgggaaa aatccaatgg gttacagcga
     6601 ttcttattgg ttcgatcgtt ggtggagtga tactaggttt gatccaaaag aaaagagtaa
     6661 ctgataaaga caagaagatg gataataaag cagcaaatcg tacagttatc gaagaaggaa
     6721 gagcaggagg cttatcatga caatcattca tgaagagaac atttttttac aacagccatt
     6781 agatagtcaa gaagaagcat ttaagttttt agcaaagcaa acattctgtt taggaatcgc
     6841 tcatgacgaa aaagaagtat atcgaaaact tctagaaaga gaaaaagaag gtactacagg
     6901 aataatgtca ggttttgcta tccctcatgc gaagtctcaa acaatcaacg aacctagtat
     6961 gattatcgtc acactgaaca aaggaattga atggaacagt ttagatagac agtcagttga
     7021 ttttattttt gcgttattta ttccagattc agaagcagga acgactcatt tgaaattatt
     7081 gtctctcgtt gcacgatcat tgatgcgaaa agaggttaca gaacagttga gaaaagcaaa
     7141 atcaaaaaca gaaatagcca caatattaac taaagaaata ggtgaagatc tatgaagaag
     7201 atgagtatag aaaagcagga acgtttgaag cgattgacgg atgaagctgg aattattagc
     7261 gcgttagcaa tcgaccagcg tggtgctttg aagaaaatga tcaaccaata taaagaagct
     7321 acatcagaag acatcatcca atttaaacag atggtttctc aatacctgac tccctatgcc
     7381 tcagctattt tacttgatcc agaatgcggc ctgcctgcag cagcgcttag agagaaagaa
     7441 tgtggcttgc tgctagctta tgaaaaaacc ggctatgata cgaaaatgcc gggaagatta
     7501 cctgatttat tgaatatttg gtcggtgaaa aggttaaaag aagctggtgc ggatgcttgt
     7561 aaatttttat tgtactatga cgtggatgaa ccagcagaga ttaatgtgca aaaacaaata
     7621 gttatagaac ggattggttc agaatgcgat gcagaaggca tacctttctt tttagagatc
     7681 ctttcttatg atgcaactat tggcgatccg tcctctcttg cctatgcaaa agtaaaaccc
     7741 cataaagtta ttgaagccat gcgagaattt tcaaaagaac gttatcaagt ggatgtattg
     7801 aaagtagaag tgccagtgaa tatgagattt gtggaaggat atgggcaaga gacttgttat
     7861 agtcaagcag aggcatccgc gtatttctat gaacaatcga aagtgactga acaaccgttt
     7921 atttttttaa gtgcgggagt caatgctacg ttatttcaag atacattacg ttttgcaaaa
     7981 caatcaggtt cgacattcaa tggtgtctta tgtgggcgag caacatgggc gaagggagtg
     8041 gcaccttttg ttaccgaagg cgaagaaaaa gcgagtagtt ggatgttaga agaaggaaaa
     8101 caaaatatta aggaattaag tacactatta aaagaaacag caacacctgt ctacgttgat
     8161 agataagaaa aagttgtttc ttcataaaaa gatgctgctg caattagata gcgatcaatc
     8221 gatgattcaa attatacatt cacttaaata aaaatatcga ctagatttct ttttcaaaaa
     8281 tcttggtcga tatttttaat tgctaaaact gttaatgaat ttttattaca ctcttattta
     8341 ctatctactt ccgcttctgc tgctttcctg cgttgcggcc tttgttattt gtactgtttt
     8401 ttggttgttt gatttgtttt gttgcttgtt tgttgtcagc tggttttgct gattctgtca
     8461 cgtcttttct tggagtgacg acttgttttg gcgggttttg tttcaactct tctgccactt
     8521 gtgctttcaa gcgtggtttc atcataacgt ttgtgatgaa tgtttgcaga cagctgaaca
     8581 caccaccgac tacccagtaa agtgtaacgc cggctggcgc actgatcgac atgaagacga
     8641 tcatagcagg agagacgatc agcatcgtac gcatcgtctt tttctgttct tcaggaatac
     8701 cgattgttga aatatatcct tgaagcagat aagcgacccc tgcaatggcc acaagcacca
     8761 tacttggtga gcctagatcg atgccgtaaa aagatgattc acggatacct tctgtgtagc
     8821 gagccgtaaa atacaaggca gagaagatcg gcatttggat caatagaggc agacagccga
     8881 ttccgccggt catactcaat ccgttttcgc gatatacttg ctgcatttct gcttgtgctg
     8941 ccatttgttc ttcacgtgtt gttgcagctt ttagcttttg ttgtgctgct tcaacttgtg
     9001 gttttaacgc ttgcattttt tcagattgga tcattgtttt tttcgattga ctgataccta
     9061 gtggcaagat gatgatacga acgatcacag tcattgcaat aactgcccag ccgtaagacc
     9121 aattgaaatt ctcaactaga tatgtaatcg cttggcccat aggatggacg agtactcgat
     9181 aaacgattcc tgaagtgtca gggtttccgt ttgaatccat ccgcacacag ccagataaaa
     9241 agagcaggat actgacaagt ccggatccca ataaccagtt tttccattta ttcatagaat
     9301 acttcctctt ctataatttt tacacaaact taactatact aaaaaagcgc atggaattca
     9361 atcaacttca tagaaatttc atataaatat tctttctttt tcgtagcgaa aaattttgaa
     9421 aaaagacaaa aaatcaagaa agaaatactt ggaaagaaaa gagtgattcc aagtgtttct
     9481 ttcttatata aaaaactaaa aacttttttg ttctgtctgg aaaatatggg ttaagcaaat
     9541 ttattgcaag ttatagatca gcttttttac tacctatctt taatacttga ggcaaagcgc
     9601 ctcaaaaaca acctgatcga cggtgttcaa aagtcagttt ctcggtatta agtcaaattt
     9661 atgcaaaaat gtggaaagcc attttcacaa aattttcctt aatactcaaa actaagcgac
     9721 cttttcacaa ccttatccca ccacatcaaa tttctttcgt tcctcaatca tcggatcttc
     9781 ttggacatcc acatactgca ctcgtccact tgggctcgga gacttcttga cttcttcgat
     9841 aaatttttgg atgatgtcgt catttccgat tgcttcgatt gtgactgagc cgtcgtcttc
     9901 gttttttacc gttccggtca cgcctaactg atcagcaacc atcttggtca tatagcgaaa
     9961 tcccacgccc tggacacgtc cttggacatt cattttaact ttgcgcatgt tcgtcacctt
    10021 tctttcctgt tttataaatc aatgataccg atttttttag gatgaaacaa gctcaagcac
    10081 ctattccatg atataatcag gtgaggtgaa ggaatgtgaa agagatccaa tcagcaaaaa
    10141 atccaaaaat caaagaatta aaaaagctac ataaaaagaa atacagagaa gaaaaggcag
    10201 aatatctttt ggaagggttc catctgatcg aagaagctgc gtcgtctgat gcagaaataa
    10261 aagaactttt tgtgacacag agaggtatga aagaatgggg agactggctg gctgtaaaca
    10321 aagagatttc tacttttcta gtttcagatg aggtcatgac cgctatgtca gatctaccta
    10381 caccgcaagg gatcttagcg gtgatgacgt taacatctga agatacgcca gatttttcag
    10441 gaggctggct tttactagat aatgtacagg atcctggaaa tgtgggaacg atgatccgta
    10501 cggcagatgc gtttggtatg gctggtgttt tattaggtaa aggaacggca gatatttata
    10561 gtacaaaggt gctgcgaagc atgcagggaa gcaattatca tttgcctgtg ttacaaaaag
    10621 atttggcaga agtgatccct gtaatgaaag gcaaagggtt tacggtgtac ggtacagaat
    10681 taaatgaaga agcacatgca ttagacaaag tagaaaaaac agaggatttc gctataatca
    10741 tgggaaacga aggtcaggga gtaagtcagg aaatcttgtc tttgactgat cagaacgtat
    10801 atatcccaat gaaaggaaat gcggaatcat tgaatgtcgg agtcgcagca gggatcgttc
    10861 tttatcattt ttctttatga ttaaggaact attaagcttt agctatttta tctagagcga
    10921 ctagaataat ccgaacactg gtggtatact tagatcaatt tcagaagaaa atgaaattca
    10981 aaaaggtggc agatcgataa ttatgacaga aaaatggcgt gaagacgaag aatatatgtc
    11041 ttatgtacaa gatcttttag aaacagaagc agtgaaaaaa ttagcgaatt acacacagca
    11101 tgttcattcc actcgtttgg agcattccat cagtgtttct tactatagtt atctgctcgc
    11161 aaaaaaatgg ggtggcaatg ctaaagcaac agcgcgtgct ggacttttgc atgatctgtt
    11221 ctactatgat tggcgtacga cgaaatttga cgaggggaca catgcgtata tccatccaag
    11281 aattgctgta aagaatgcag aaaaaatcac cgatttgtcg gatcttgagc gagatatcat
    11341 tttgaagcac atgtggggtg caacgatcgc accgcctaag tataaagaag gatatattgt
    11401 gacattcgtg gacaaatact gcgcagtcaa agaagcggct cagcccatgt ctgcaagtat
    11461 gcgtaagaga tggcaacgtt actttgggaa agaatcctcg atttaacagg taaaaccaat
    11521 aatagaccat attgaggttg tgacagaagt ggtcagcttc aagaaataag aaggaattta
    11581 cgaaaattac ttctcaaatt tttgtgaagt ttcggcttat taccgcagat gcaagctttt
    11641 gaacaccatt cattcgaaac aagggacttt gacacgactt ttgtcacagt ccttttttct
    11701 gtttgcagtt gactatctgt tcaagatacg gtttactttt atttatcgaa tgaatcatgt
    11761 acatagaaga ataagcatta aagggggtga tgataagatg caaaacgtat cttatttaaa
    11821 agagtccggg atcttcaggt aacacatact ttgcagaaga tcactttttt ctacaaagta
    11881 tgcactttgg ttagaagaaa ggactttaaa atcatggaac aaacaataaa accataccaa
    11941 tattttttta taaaaagaga agtagaacag ttattaaatg cgtactcatc tgtcaatgat
    12001 ccaaaaacgg tacaaacagt tcaggcacta gcaactgaaa agattcgaga catattagaa
    12061 cacgaactgc cagaaatcga tacattttta acgacagttt tagacgtaaa actgacaaaa
    12121 gcgcaagcag aacgagcatt ggaggagctg aagcaaatcg ttcagccgtt tatgcagcct
    12181 agcaaaccac aaatcgagaa attattccga aaagtgaaaa aactcaaaca accagcttgg
    12241 aatgaaatcg acttgcaaga acatacgtac atcggctgga atgatccagg tacgcagaaa
    12301 aaattcatga tctattacga agatgaaaaa ttaaaaggga ttagtggtac tttatcacca
    12361 acgataaata agggaatctg tgcaatctgt cagaaaacat cgaatgtttc gctttttctt
    12421 tctacaacga aagcaggttc tgatggcacg tatacgaaaa aggggaatta tatttgtcat
    12481 gatagtgacc aatgtaatca acagttaacg aaattggctc ctttctatga atttgctgaa
    12541 cgtgtaaaga aagaacgata attttattgt tttttacaga aaaatatgct atcttttaat
    12601 ggaagtgaat aggtgatggt gttcgccttt aaatgctaac gaaagatagc taatgacgcc
    12661 tactaaagtc tggggacagc ccctttcttt agtaggcgtt tttttattga ggaggaattt
    12721 tttatggaaa aacaattacc gagtccagta cgcattttag tgtacatgct gaagtggctg
    12781 gtgatcacag cgattctcgg tatattcatg gggagcttgt ctgctttttt cttaaatagc
    12841 ctgacagttg tgacagatat ccgactatca catccttggc tcctttatct actaccagtt
    12901 agtggtgcgc tttttgctta tctctatgcg tatcacggcg gtctttctag tcgtggaaac
    12961 aacttagtga tcgatcaagg aaatggtgga gaagaaaaaa tcccgttgcg acttattcca
    13021 cttacactgt ttggaaccat aactacccat ttatttgggg gatctgttgg ccgtgaagga
    13081 acagcagtcc aaatgggggg agcgttagca gataatacag cacgtttgtt ccgattggat
    13141 aaatcagaac gggaaatctt gattatcagt ggaatcagtg cgggttttag ttctgtattt
    13201 ggcacacctt tagctggaac attatttgga ttagaagtgt tggcaatcgg gaaagtccga
    13261 acagaagcga tcttccctag cttctttgct gcactgtttg caaatttcat gacagaaagc
    13321 tatggtgttt ctcatctaca ttaccaaatg ggtgtgattc ctgaatggag tatgttgctg
    13381 ttcttcaaag tttttgttgc tgggattgct tttggtttga ttggttgggt attcagccgt
    13441 tcgatcgtct ttttaaaagc gcgatatgca caatggattc ctcatcctgt ttggcgaaat
    13501 acgataggtg gagctgtagt tgtggcagca gcattgattt tgcaaacaca acgttatctc
    13561 ggattgagtc ttccgttact tcaagaatca tttaatggac aggcacatgt ctatgatttt
    13621 attgggaagt tattcttcac agttttgtct ttaggcgcgg gatatcaagg gggagaagtg
    13681 acaccattgt ttgaaattgg tgcaacgctt ggtgcagcac tagcgccttt gctccatgtt
    13741 tctgttcctt ttcttgcagg attgggcttt atcggagtat tttctggtgc gacaaataca
    13801 ccgattgcct gctttgtgat ggggatcgaa ttgttcggtt cacaagcggc agtatacttc
    13861 tttatgatct gcttgatcag ctttatgtgt tcaggcaaca gcggtattta ttctgctcag
    13921 caagtagcgg tcaaaaaagg gatactcttt ttgccaatag ttaagagaat gaaagaaaaa
    13981 aacaaggtgt gacatatgtt ttgtcacacc ttgttttttt agaaaagtaa agtaatcagc
    14041 gataagaatg cggagcctgc gaaaagcaat ccgcaaatga tcatatgtct ttttttgttc
    14101 atcatcaaga tggcgatgta ttctcttaaa aacgcattag gcaagtagta gaaagctgtt
    14161 tttgcaccaa taccatttgc tttcaatttg gcttgccacg cataaagacc ggctctaaaa
    14221 atgtggtaat tgttggaact aaagatcgcg cggtatggcc ctttcatttg ctgatccatg
    14281 atttcctttg aatagagcat gttctccaaa gtggttgtcg aattggtttc gacaagaatg
    14341 tctctttcag ggattccttg ttccatggca tattgtttca tcgcaatggc ctctggtact
    14401 ttttcgtctg ctccttgccc gccagacatc aataagatgg gcggatttaa tgtagcacgg
    14461 ctttgagccc gataaaaagc aattgcctta ttgattcgct tggctagaag aggagaaact
    14521 gtttctccat tgatcaaccc agcgcctaac acaacgatga agtcttgatt gtatcgaggc
    14581 cggttgaact gatacaagaa tgagacagtc aaaaagttgt agaatacgat aaatagatag
    14641 atcaagatca ggggaacgca gaaaaacaaa gcatttatcc attgcggcaa atattttaac
    14701 aagaaaaagt caaaaacaag aaacagtgta agaaaaatgg ctagtatcaa cgttaaaaga
    14761 ttagctaatg aatgcgattc tctacgtaaa actgtgaccc cgttccaaaa aagaaaaaca
    14821 atcaatccat aaataccaaa taaaaggaca agtagcaatg gaatcgtgat caaggcgatc
    14881 agtccgccaa ggaaaatatt ttgcgtttca tacagcaaga caaacaagta gatgccgaaa
    14941 ctgatgagga aaacattgaa taaaagacca ttcagcagcc ttcgtttttc tgtgaaataa
    15001 gaaaaagcaa agatactaaa gaacaacaat ggaatcaaaa aataaaaggt cgaataaaaa
    15061 atttccaaca cgacaaaata agttaagatt accgtaaatg tccacgtact gccgtaaaag
    15121 aacaaggaag gatccttctt ctgttttcgc caataaacga tagagaatac cccaataaga
    15181 tagagaacta aaaagatcag atctctggtt agattcataa attcaactcc tttttcttat
    15241 acagtataac gaaaatagat gaaaaaagct gttatttaaa ctagaaaaag caaaaaaaca
    15301 tctttccgat ggaaa
//
LOCUS       DACDRN010000055        15239 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDRN010000055 DACDRN010000000
VERSION     DACDRN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.055"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..153)
                     /locus_tag="IWJ80_002033"
     CDS             complement(<1..153)
                     /locus_tag="IWJ80_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9461741.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            236..385
                     /locus_tag="IWJ80_002034"
     CDS             236..385
                     /locus_tag="IWJ80_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HAP9461742.1"
                     /translation="MKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPL
                     ENELR"
     gene            complement(1134..1747)
                     /locus_tag="IWJ80_002035"
                     /pseudo
     CDS             complement(1134..1747)
                     /locus_tag="IWJ80_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406632.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(2968..3672)
                     /locus_tag="IWJ80_002036"
     CDS             complement(2968..3672)
                     /locus_tag="IWJ80_002036"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012610.1,HMM:NF019322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9461743.1"
                     /translation="MIFDKVKEQIRKVYITTDRKSELLPSERDLAQEFSVSRPTIRKA
                     LASLEDEGIISKNEGRGYLVKPASEKYVDHELDSFIGFFQDAVQQGKKISSKIIQQTI
                     IPADSTISECLEIEQNTLVLVLERIRYIDDYPTCIAKSCIPLSVLPEIVEHDFAKESL
                     FSVLESRGIRLAYAKRSIEVTPPTDLENLYLDLEKNEPVFVFTSIGFAKNQKPFEYEI
                     SKYPAYKTKFESTVSK"
     gene            complement(3687..4556)
                     /locus_tag="IWJ80_002037"
     CDS             complement(3687..4556)
                     /locus_tag="IWJ80_002037"
                     /inference="COORDINATES: protein motif:HMM:NF012585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461744.1"
                     /translation="MKIAVICSGGDSPGMNSALLAIKKTIDSEPDSEVVFFKNGYAGI
                     FKECIASVSLKEVEVFKAGTLIGTARFEKMRDLESRKIIIENLNKMEIDNLIVIGGNG
                     SLKGAELLKNESELQVSVIPGTIDNDFPNTDYTLGFDSALNTIIRSIDDLTSTANAHD
                     RIIIMEVMGRGCFALPEYASIANNTYWVERDDKEYLHLICKKFESEFYSPILLLPEGI
                     SNKQKIIEFITKNLPKETKYLNLAYLQRGSSPTFYDRYIGYLWGQLAAKELIEGKSGI
                     LSQKNGNIMLIPW"
     gene            complement(4664..5680)
                     /locus_tag="IWJ80_002038"
     CDS             complement(4664..5680)
                     /locus_tag="IWJ80_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9461745.1"
                     /translation="MFNFDQERFLKTQAGAVATNEQISNILESIASDLEEIYFIGSGG
                     VGILFDSTIEFARTRSTFPFHRVIAAEFMIHPNKTFSNKSLVVIPSLSGTTKETLELV
                     EYCNEMNVKTLALVGSPKTPLEKAATYSIYNDVLDDTSSENYYLQGLLIALFLMKQRN
                     EIDINQYNTYIEDLKKIPEALVHAKEQAEPMAKSFAKKHKDTSYHILTGAGDSFAEAY
                     YYGMCILEEMQWIKTRPVHAADFFHGTLELIEAGVSVIITEGEDDSRPLTERVKNFVP
                     KFTDVHVTVDTKDYDLPGISEDTRKIISPVVLATVFERISAFLEEERNHPLTTRRYYK
                     QMEY"
     gene            5870..8593
                     /locus_tag="IWJ80_002039"
     CDS             5870..8593
                     /locus_tag="IWJ80_002039"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012385.1,HMM:NF013070.1,HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9461746.1"
                     /translation="MKTKILKELNDLLNSYDFPQYFPEECNAQYIAQKFQIKRNTASK
                     HLNELMEQKKVVKINGRPVLFFPVHTIERYISHSLDSFEFSSIDSFREKISKKKADHF
                     FDDLIGAKQSLKEAVQQGISAMKYPGGLPIMYFGSSGVGKSYLVEKLAEYCRQTGIIH
                     ENAPFIELNCAQYFNNPELLTSQLFGHMKGSFTGADSDHIGIIEAADGGIVFLDEIHR
                     LPPEGQEKLFIHMDKGVFRRVGDSGPWRLSHVRYIFATTEQEENFFLETFRRRIPIVC
                     TLPNYQDREISERKEIVFHLLEKESTLINKEILVSEAALSFLIRLDLYGNIGELDGLI
                     KQTIANKLFSGSNKKYIEIHISDLPSRYLLTENIELKNQIASQRFYIFSNGNYVHDCD
                     ETSTVTTNLRGLIHRVSKYYSECHDVNRNSEEFRRFVKDQMAEFNDRFIYQKNSEVLV
                     KYYLPEIQNIVQKISPSLFVHLSGNTVQAIATYLAIRDQWGYGKENASANEIEKLYEN
                     LKCNYLTDLNVFLLIFENALDTTLTIWDKLYISLCVISETGNEAKDINAIILAHGYAT
                     ASSIANVANRLVNQFVIDSIDMPLDISFSEVIDRLLHYIEYRKPKDGLVIFVDMGSLA
                     QIKTEIEQVIEVPTMIINNVTTEMAIETAQLIQSTSDIQKVVKKLPYSQFEKQVLYPI
                     KIRKRTIVVSCNTGLGTSIKIKEMMENNLSKDLGIEFLPYENETLRDTQQLEFLIKTK
                     DIIAIIGTDDPRVSNIPYISLEELFDGEIGKLYKVLLRVYGKEIAEAINQNIVKNSSL
                     NRLIDSLTILDAEKVVNQIEESLKDYQMRVGKTLSSLSKINLYVHISCMIERLIRNQG
                     VSSYPAIDQFLKRYETEVASIQKALSKIEKLYNIKIPMEEIAYVCNIIVSY"
     gene            8721..9143
                     /locus_tag="IWJ80_002040"
     CDS             8721..9143
                     /locus_tag="IWJ80_002040"
                     /inference="COORDINATES: protein motif:HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose IIA subunit"
                     /protein_id="HAP9461747.1"
                     /translation="MRQILCMSHGPLAKGMIETLSIIVGDLDNIDFRCAYVDGNNDVE
                     MIIDDYLKESMGNETIVVTDIFGGSINNEWLRRLPDLSKVYLISGMNLSLLVDLYLKF
                     QLVSSDQIEEIIDHAIQASSGSIKFCNQLDYEQTVDDF"
     gene            9161..9631
                     /locus_tag="IWJ80_002041"
     CDS             9161..9631
                     /locus_tag="IWJ80_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9461748.1"
                     /translation="MIKLVRIDHRLIHGQVALTWTNAISADCILVANDEILNDPIQKT
                     TLKLAAPHNVKVVIKSIQDSIDAINSGKTDKYKLFVVTKTISDAKRLANGVEQIKSVN
                     IGSVKETSGSSALTHTIFVTEEERAELKGMVSEGIEVFAQQVPTDKAIDLKKSL"
     gene            9655..10437
                     /locus_tag="IWJ80_002042"
     CDS             9655..10437
                     /locus_tag="IWJ80_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9461749.1"
                     /translation="MDSLLVQAILLGLLTAFIVIDWMTGTNNLSRPIVSSVFVGLIMG
                     DLKMGIMMGATLELAFIGAITIGAARPPDTVSGGILGTAFAIATGKGAEFALTLAFPI
                     AALFLLVDNLLTLLVLPMIARKADRYAEKGELKNVSKMHVLGFILVKSLPRALFVGFS
                     FYLGSPVMERILNAIPTFVQDGINIAAGIMPALGFAILLQMLLKKDVFVYFVIGFALF
                     SYLHVPILGIAIFGACLAYIIVNMETKAAMGSRGEEMVEDDF"
     gene            10427..11251
                     /locus_tag="IWJ80_002043"
     CDS             10427..11251
                     /locus_tag="IWJ80_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9461750.1"
                     /translation="MTSSGKALTKDDLTKICIRSCALEASWNFERQQHMGFAFAMMPA
                     LEKIYSGNEEEKQKAAQRHLEFFNTAPPLSTFIMGIVASMEEENAKNPDDFDTSTITS
                     IKTALMGPLAGIGDSMFWGTLLVIGIGVGASLAKEGNILGPFLFLLIFNVPNFLLRYF
                     GVMQGYNFGTKLLENIESSGVMKTLTHAASILGLMVIGGMSGSMVSIKVAGSIGQGDE
                     ATKIQSIIDGILPNLLSVLCVFLIYWMLKKGFKTTYVLFGIFIFGIIAAFFGFLTT"
     gene            11286..12284
                     /locus_tag="IWJ80_002044"
     CDS             11286..12284
                     /locus_tag="IWJ80_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9461751.1"
                     /translation="MLNYNAKEMKKKGKRIYGIREDVEKIAKQAYDSGISNIFFTASG
                     GSVAVMQPFEYVLMTESSIPVYSMTAAELVLMGHKQLNEKSLVILYSKSGDTKETIKA
                     AEYLKERNVPTVGIVSKEDTPLEALSTYAINYLDGRPQEMPLYFLIMSIMNLNGEFPE
                     YTRFADELKNLPTALNSVREQADEKGKKYAEEFKEDPYQIWIGSGNLWGITYSYSMCV
                     LEESQWLRTKSVSSPEFFHGTLELVEKGVCVTLLKTEGETRLLDARVEKFAVQYTDKF
                     NVFDTKDYELPGISDEFRKYFCAPVMWAALGRVSVHLEDIRNHSLDKRRYYRVVEY"
     gene            12301..13155
                     /locus_tag="IWJ80_002045"
     CDS             12301..13155
                     /locus_tag="IWJ80_002045"
                     /inference="COORDINATES: protein motif:HMM:NF012515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461752.1"
                     /translation="MTAYVIGFGDNVVDYYENLNQKFPGGNAVNFAVNAKKSGVKAYY
                     VGSLSSDPDGVLLQHAISSEGVDISFSEVIDEPTEQAHVTIKDGDREFIGGVRGSRLT
                     PALSAELIDLMKNSSLVHTSCHSRTETKIKELKKYDVKVSFDFSDLDKYRTHEYLSDV
                     CPSIYIAQFSVATDDQEEINRLISECKDFEVPYLLFTRGSKSPVFVDTRENKTYEGFI
                     RKVESPHDTMGAGDAYFAAFASNFVLSKREKSMIEHVIDCFHKGANLAYETLMIDGSF
                     GHATPINP"
     gene            complement(13729..14979)
                     /locus_tag="IWJ80_002046"
                     /pseudo
     CDS             complement(13729..14979)
                     /locus_tag="IWJ80_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         4894 a         2424 c         2924 g         4997 t
ORIGIN      
        1 cacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccattt ctaatatgaa
      241 agcaattatc aacagtaagg attacgaagg atataaagcc aaacatccag aagcgaaacc
      301 atttaaatac ccacaagaaa tgaagcgagc atggcgtaaa atgttagatg atgagttgat
      361 tccacttgaa aatgaattga ggtaaatgag aagagttaag aaaattgtac gattcaaaga
      421 tagtagtaaa gtggaagtgt agaaaatagg gatagatgtc ttttatttgt tcacatttta
      481 taacgaagta tttaagacag ctattgaata atgtttcctc cacctttttc attcatttac
      541 cccaccccgt tgttatatga ccgtgtttcg agagaaacca ataaaaagca taggctaact
      601 aggggttcct ttttcttttg attttttgga atccgcttaa gtgaaatgta tctgaaatga
      661 aacagagata aagtgatttt ttgctgtaat gacaaggttt tagctgtttc agataccatg
      721 taacccaaaa tagtttattg agaaaatagg agagtatttg atactaacta gtaggaaaaa
      781 atgagataaa aagtaaatgg aatcatttca ggtaatctca aataaaaaat atacaggcct
      841 gtggcacttt acctacggga ctgtatattt tttactctca gcgggcttgt tttgactccg
      901 tgtttgactc cgtcgtattg attttaaatg atattcgatg accaattaat aggtcgtttt
      961 tgaaattttt gtctctatat gactcagttt gataattatt gatagatcga tttgggtcta
     1021 tatcgaacaa gggaagattg caaaagtcga agcttaaagg attgatatta aaggattaag
     1081 aggtaaaaag gtatccccat aatatcctct gactccgttt ttgacctcgc ttatcatccg
     1141 ttcatgtaag aatcaaataa ttcgactgtt tcattttgcc gattttttga tactttaata
     1201 taaatgtcca tgattgtttt agaagaagaa tgtcctagcc tatcttgtac atcttttact
     1261 tgagcacctg cttcaaagag aagggtagca tgagtctttc tgaagccatg gactttgatt
     1321 atcttcatat ctgggaactt ccgtgctact ttatccatga catggttagg gtagcctttg
     1381 taaaggaagt cccttttttt attccctttg atagtgctga ttacaggtat atcatctata
     1441 ttatctatcc gaatccctct agaaaggttc ttttatgtta cagaacgttt ccatttttta
     1501 gaatacgtaa ggttccatta tcaattgaaa ttactccctt tgatttgtct gtttttgtag
     1561 gattaataca tagagagccc tcggatagtt ctactaacgt tttgttgaag cagatagttt
     1621 gattatcgaa atctatgtct ctacatgtca gtccgagtat ttctcctttt cgttcccctg
     1681 tataagaagc tattctaaag aatactaaag cacgctcatc ttttaactta tgcaaacatt
     1741 caaaaaacag atatttcaca tatatctaaa attcggagat tgaaagaatg aatttatcta
     1801 agaattttca ctaatgttgc gaatataata tcagaaaatt tcgaaataaa ttttgttaaa
     1861 aattagaaaa atccgaatac ttagtagtgg ataaattttt ttcaggaata gtacgtttta
     1921 ttaaaatgaa aactaatccg acatcaactt ttttatcgaa gcaaacatca agcggtaaaa
     1981 agacctcttt agtttgaaaa gtgttgcttt catattgatt taaattttgg ttaagtaggt
     2041 aaattaagaa atgatcagtg taagtatttc ctgatttagc gcaccttgtt cactttgaaa
     2101 atacaggttt ttgatggaca gattttgctt caaatgttta aagaacatgc cactgtactt
     2161 ggtacaggtc ggcgccccca tttggagttg cgtcagatcg agtcgtgacg attcattttg
     2221 tacaaattag aatttaaaat tgaatacatg acgcgcacat tttcccagag agccatattc
     2281 tgtaattaat cacagcaatt agtgattatg cacaatctaa aaaactgatt tataataaaa
     2341 atattattca aataaataat aataactgcc cgcatgatgt tgacagttac aatagcacat
     2401 agatagactg gcaaagatgg ttcttaatct ttcactttgg tcagaattct tgggttatcc
     2461 ttccagtgaa agtcagcaga aaatccttaa tgcttacaaa taatttttgc gaaataggtt
     2521 ttattggtaa aggactcaat catgaatatg aattactttt atctggtaaa tttggaaaaa
     2581 ttttgcaaag aaataaaaat tagacaaatt tgctacacat aatcaaagat taaaaaagtt
     2641 tttaaaatat agttgtttga atggggatat gaattagaga aaattggttg attcaacggg
     2701 tgctatcatt tttgatagcg tcatatgtga agtaagcaca cctaatgaaa aaggttcagg
     2761 cttgctttga agacttatta acaactatgt cgactatcct tgacttaggg tagaggagta
     2821 cctccacaca ccttaattag atgataatgt ttggactgta ggctacttag cggattaaga
     2881 taaagaggaa gtagaaaaag agccttgcgg aaagacactt tatctgttat ccgcgaggct
     2941 ctttatacat ttaagcatat ttaaaaatta tttggaaaca gtactttcaa actttgtctt
     3001 ataagcaggg tacttagata tttcatattc aaacggtttc tgatttttag caaatccaat
     3061 acttgtaaag acaaagactg gctcattttt ttctaaatca agataaaggt tttctaaatc
     3121 tgttggcggt gtaacttcaa ttgagcgttt ggcataagct aatctaattc ccctactttc
     3181 taatactgag aacagggatt ctttggcaaa atcatgttca acaatttcag gtaaaacaga
     3241 taggggaatg caactttttg caatacaagt tggatagtca tcgatgtatc gaattctttc
     3301 caagacaagc actaaagtat tctgctcaat ctccaaacat tcactaattg ttgaatcagc
     3361 aggtatgatg gtttgttgga taatcttaga actgattttc tttccttgct gaaccgcatc
     3421 ttggaagaat ccgataaaag agtcgagttc gtggtcaaca tatttttctg aagctggttt
     3481 cactagatac ccgcgacctt catttttaga aataattcct tcgtcttcta aagaagctaa
     3541 agctttccga attgttggac gactcaccga aaattcctgg gccaaatctc tttccgaagg
     3601 caataattca gatttccgat cagtggtgat atacactttt cgaatttgtt cttttacttt
     3661 gtcaaaaatc atagtaatcc atccttttac caaggtatta acattatatt accatttttt
     3721 tgacttaata tcccagattt tccttcaatt aattccttcg ccgctaattg accccacaaa
     3781 tagccaatat atcggtcata aaaagttgga gatgatccac gctgaagata cgcaagattt
     3841 aagtatttgg tttcttttgg taaatttttg gtaataaatt caatgatttt ttgtttgtta
     3901 gatatacctt ctggtaaaag caatataggc gagtagaact ccgactcaaa ttttttacat
     3961 attaagtgta agtattcctt gtcgtctctc tcaacccaat aagtattatt tgcaatggaa
     4021 gcgtactctg gaagagcaaa gcagcctcgg cccatgactt ccattataat aatacgatca
     4081 tgagcattag cagtagaagt taaatcatcg attgatcgga ttatagtgtt taaggcgcta
     4141 tcgaagccaa gtgtataatc ggtattgggg aaatcgttat caatagtccc tgggatgaca
     4201 gatacttgta attctgattc atttttcaat agttcagctc ctttcaagga accgttacct
     4261 ccaataacaa ttaagttgtc gatctccatc ttatttaggt tttcaataat aattttccga
     4321 ctttcgagat ctctcatttt ctcaaatctg gctgttccaa taagtgttcc agctttgaat
     4381 acctcaactt cttttagaga aactgaggca atacattcct taaatattcc ggcataacca
     4441 ttcttaaaaa agactacttc cgaatctggt tcgctatcaa ttgttttttt tattgctaat
     4501 aaagctgaat tcattccggg agagtcgcca ccactacaga taactgcgat tttcatttta
     4561 gatcctccta aaaaaggtga ctgcgattct attctcccat tagagaaaat gaagcaatca
     4621 aatgggcaat ggagtcgcgg tcaccttttt catattttta tgattagtat tccatttgct
     4681 tataataacg gcgagtagtt aaagggtggt tacgttcttc ttctagaaaa gcactaattc
     4741 gttcaaagac agtagctaag acgactgggg aaataatttt tcgcgtgtct tccgaaatgc
     4801 caggtaagtc atagtccttt gtatcaaccg ttacgtgaac atctgtaaac ttaggaacga
     4861 aattcttcac gcgttctgtg agaggtcgag aatcatcttc tccttctgta atgatgacac
     4921 ttacacccgc ttcaattaat tcaagagttc catggaagaa gtctgctgca tgaacaggac
     4981 gagtcttaat ccattgcatc tcttctaaaa tacacatgcc atagtaatat gcttccgcaa
     5041 agctatcacc agcaccggtt aaaatatgat atgaagtatc tttgtgtttt tttgcaaaag
     5101 attttgccat tggttccgct tgctctttag cgtgcactaa ggcttcaggt atttttttta
     5161 aatcctcaat ataggtattg tattgattga tatcgatttc atttctttgt ttcattaaga
     5221 agagagcaat cagcagtcct tgaaggtaat aattttctga agatgtatca tctaaaacgt
     5281 cgttatagat agaatatgtt gcagcttttt ctaggggcgt tttaggactt cctaccaaag
     5341 ctaatgtttt gacgttcatt tcattacagt attcgactaa ctcaagtgtt tcttttgttg
     5401 taccagataa agatgggata acaaccaaag atttgttaga aaatgtttta ttgggatgaa
     5461 tcataaattc ggcagcgata acacgatgaa aagggaaagt gctacgagtt ctcgcgaatt
     5521 cgattgttga atcaaaaaga ataccaactc ctccagaccc aataaagtaa atttcttcta
     5581 agtcagatgc gatggattct aaaatattgg aaatttgttc atttgttgca acggcaccag
     5641 cttgagtctt taaaaatctt tcttgatcaa aattaaacat aaaaatctct cctttgatta
     5701 ccaatggtaa tattttgttt gcataaataa aataccacag gtaataaaaa gtgtcaaata
     5761 ttttttagga aaaataccat aggtattgtt aaagtggaaa gatgtttata aagaatattg
     5821 aattttatag tgaaaatcat ttattattaa gtaaaggagg tgggttcatt tgaaaacgaa
     5881 aattttaaaa gaattaaatg atctacttaa cagctatgat tttccacaat attttccaga
     5941 agaatgtaac gcgcaatata ttgcccaaaa atttcaaata aaaagaaata cggctagtaa
     6001 gcatctaaac gaactaatgg aacagaaaaa agttgtgaaa atcaatggtc gtcctgttct
     6061 tttttttcca gtccatacaa ttgaacggta tatatcacat tcattagata gttttgagtt
     6121 ttcttcaatt gattcgttta gagaaaaaat ttcaaagaaa aaagcagatc atttttttga
     6181 tgatttaatt ggtgcaaaac aaagtttaaa agaagcggtt caacaaggga tatcagcaat
     6241 gaaatatcca ggtggattac caataatgta ttttggatcg tccggagttg ggaagagtta
     6301 cttagtggaa aaactagctg agtattgccg acaaactggt attattcatg agaatgctcc
     6361 gttcatcgag ttaaactgcg ctcagtattt taataatcca gagttattaa caagtcagtt
     6421 gtttggccat atgaagggat cgtttaccgg cgccgattct gaccatattg gtataataga
     6481 agcagcagac ggtggaatcg tctttcttga cgaaattcat cgtttaccac cagaaggaca
     6541 agagaaactt tttattcata tggataaagg cgtttttcga agagttggag atagtggtcc
     6601 ttggcgttta tcacatgtta gatatatttt tgcgacaacg gaacaggaag aaaatttttt
     6661 tctagaaact tttcgacgaa gaattccgat tgtttgtact ttgcctaatt accaagatag
     6721 agaaattagc gaacgaaaag agattgtctt ccatttatta gagaaagagt ctactctgat
     6781 caataaagaa atattagttt ctgaagccgc ccttagtttc ctgattcgct tagatttgta
     6841 tggaaacatt ggtgagctgg atggactaat aaaacaaact attgcgaata aactatttag
     6901 tggaagcaat aaaaaatata tcgaaattca tatttctgat ttacctagca ggtacttgct
     6961 aacagaaaat attgagttaa aaaaccaaat tgcgagccaa cggttctata tctttagtaa
     7021 tggtaactat gttcatgatt gtgacgagac ttcaactgta actacaaatt tacgaggttt
     7081 gattcatcgt gtttctaaat attattcaga gtgtcatgat gtgaatagaa atagtgaaga
     7141 gtttcgacgt tttgtgaagg accaaatggc agaatttaat gatcgtttta tttaccaaaa
     7201 aaattcagaa gtcttagtta agtattattt accggaaatc caaaatattg ttcaaaaaat
     7261 ctctcccagt ctttttgttc acctttctgg caatacggtc caagctatag ctacctatct
     7321 cgctattagg gatcaatggg ggtatgggaa agagaatgct tcagcgaatg aaattgaaaa
     7381 gttatatgaa aacctaaagt gtaactattt gacggattta aatgtattct tactgatctt
     7441 cgagaacgcc ctggatacaa cattgactat ttgggataaa ctgtatatca gtttgtgtgt
     7501 tattagtgag accggaaatg aagcgaaaga cattaacgca attattttag cccatggata
     7561 tgcgacagct agtagtattg ctaatgttgc caatcgtttg gttaatcagt ttgtcataga
     7621 ttctattgat atgcctctag atatcagctt tagtgaagta attgacagat tacttcacta
     7681 tatagagtat cgaaagccta aagatggact agtgattttc gttgatatgg gaagtttagc
     7741 tcaaattaag actgaaattg aacaagtaat cgaagttcca acaatgatta ttaataatgt
     7801 tacaaccgaa atggctattg aaaccgcaca actgattcaa agtacgagtg atatccaaaa
     7861 ggttgtaaaa aaattgccgt attctcagtt tgaaaagcaa gttctatatc ccatcaaaat
     7921 tagaaaacgt acaatcgttg tttcctgcaa tactggtttg ggtacctcca taaaaattaa
     7981 agaaatgatg gaaaataatt tgtcgaaaga cttgggcatt gaatttctgc catacgaaaa
     8041 tgaaactctt agagatactc agcaattaga gtttctcatt aaaacaaagg atatcattgc
     8101 cattatcgga acagatgatc cgcgtgtgtc gaacattccc tatatttctc ttgaagaatt
     8161 atttgatggt gaaattggca aactctataa agttcttttg agagtctatg gaaaagagat
     8221 tgctgaggca attaatcaaa atattgttaa aaatagctca ttaaaccgct tgattgacag
     8281 tcttactatt ttagatgccg aaaaagttgt caatcaaatt gaggaaagtt taaaagatta
     8341 tcaaatgagg gtggggaaaa cgttatcatc attgtccaaa attaatcttt atgttcatat
     8401 cagctgtatg attgagcgac taattcgtaa tcagggagta agtagttatc cagcaataga
     8461 tcagtttctt aagcgatacg aaactgaggt tgcttctatt caaaaggcac ttagtaagat
     8521 tgagaaactt tataatatta agattcctat ggaagagatt gcatatgtgt gcaacatcat
     8581 tgtaagttat tgataaaata aagtgacact attcacagcg atggaaatag tgtcacttta
     8641 ttttttgtac gccctttcat ttaagtggtg acagtaattg gcacagtatt tgctaataac
     8701 aaagcgaaag gagttcgggt atgagacaaa tattatgtat gagccacggt cctctagcaa
     8761 aaggaatgat tgaaactttg agtattatcg taggagattt ggataatatt gattttcgat
     8821 gtgcctatgt cgatggaaat aatgacgtag aaatgatcat cgatgattat ctaaaggaat
     8881 ctatgggaaa tgagacgatt gttgtaaccg acatttttgg tggaagcatt aataatgagt
     8941 ggctaagacg gttgccagat ctttctaaag tctatttgat ttctggaatg aatttaagtc
     9001 ttttagtgga tttatattta aaattccaat tagtttcatc tgatcagatt gaagaaatta
     9061 ttgatcatgc aattcaagca tcttctggtt cgattaagtt ttgtaatcag ctagattatg
     9121 agcaaacggt tgatgacttt taaataaagg agggaagaga atgattaaat tagtgaggat
     9181 cgatcatcgt ttgatacacg gtcaagttgc tttaacttgg acgaacgcaa tcagtgcgga
     9241 ttgcatttta gtagcgaacg atgaaatttt gaatgatcca attcaaaaga caactttgaa
     9301 attagccgca ccacataacg tgaaggttgt tatcaaatca attcaagatt cgattgatgc
     9361 aattaatagt gggaagacag ataaatacaa actatttgta gttaccaaaa ccatttccga
     9421 tgctaaacgg ttggcaaatg gggttgaaca aattaaatct gtaaatatcg gtagtgttaa
     9481 ggaaactagt ggcagtagcg ctttgacgca tacaattttt gtaacagaag aggaacgagc
     9541 agaacttaaa ggaatggtct cagagggcat tgaagtgttc gctcaacaag ttccaacaga
     9601 taaggcaata gatttaaaaa aatctttgta ataaaaaaat aagggggtaa tttcatggat
     9661 agtctcttag tacaagcaat tttgcttggt ctcttgactg cgttcatcgt aattgactgg
     9721 atgacaggta cgaataactt atcaagacca atcgtatctt ccgtctttgt tggtttaatt
     9781 atgggcgatc ttaagatggg gattatgatg ggggcaacac tggagttagc atttatcgga
     9841 gcaataacaa tcggagcggc acgtccgcct gatacggtat ctggtgggat tttagggact
     9901 gcgtttgcta ttgcaactgg caagggagca gaatttgcgt tgaccttggc attcccaatc
     9961 gctgcgctat tcttattagt tgataatcta ctaacattgc ttgttctacc aatgattgct
    10021 agaaaggcag atagatatgc tgaaaagggc gaactaaaaa atgtgtcgaa aatgcatgtt
    10081 ttaggtttta tcttagtaaa aagtttacca cgggctttat ttgtcggatt ctcattttac
    10141 ttaggaagtc ctgttatgga acgaatctta aatgctattc caacttttgt ccaagatggg
    10201 attaacattg ctgcaggcat tatgccagca ctaggttttg ctattttatt gcaaatgctt
    10261 cttaagaaag atgtctttgt atatttcgta attggttttg cactctttag ctacttacat
    10321 gtgccgattc tggggattgc aatcttcggc gcatgtctag cttatattat agtaaacatg
    10381 gaaaccaaag ctgcaatggg ttcaagaggg gaggagatgg ttgaagatga cttctagtgg
    10441 gaaagcttta actaaagatg atttaacaaa aatttgtatt cggtcatgtg cattagaagc
    10501 atcatggaat tttgagcgcc aacaacatat gggctttgct tttgctatga tgccagcgtt
    10561 agaaaaaatt tatagtggca atgaagaaga aaagcaaaaa gcagctcaaa gacatctaga
    10621 gttttttaac acagctccgc cattatcaac attcataatg ggaattgtgg cttctatgga
    10681 agaagaaaat gccaaaaatc ctgatgactt tgatacttct acaatcactt ctattaaaac
    10741 tgcattgatg ggaccattag ctggtattgg tgattcaatg ttttggggta ccttattagt
    10801 tatcggtatc ggtgtaggtg cttcattagc aaaagaaggc aatattttgg gtccattttt
    10861 atttttacta atcttcaatg tccctaattt tcttctccgt tactttggag ttatgcaggg
    10921 atataacttt ggtacaaagc tattagagaa cattgaatcc tctggcgtca tgaaaacgtt
    10981 gactcatgct gcctcaattt tgggtcttat ggtaattggg ggcatgtctg gaagtatggt
    11041 atcaatcaaa gtagctggtt ctattggaca aggtgatgaa gcaacaaaaa ttcaaagtat
    11101 cattgatggt attttaccaa atctgctgtc agtattatgt gtatttctga tttactggat
    11161 gcttaaaaaa ggctttaaaa caacttacgt gttattcgga atctttatct ttggtatcat
    11221 agcagccttc ttcggatttt tgacaactta attgaataat ctaaataatt gtaaggagag
    11281 aactaatgtt aaactataat gcaaaagaaa tgaaaaaaaa aggcaaacgg atttacggta
    11341 ttcgtgaaga tgtagaaaaa attgcaaagc aagcttatga ttctggaatt agtaatattt
    11401 tctttactgc ttctggtgga tcggtagccg taatgcaacc atttgagtat gtattgatga
    11461 ctgaatcaag tattcctgtt tactctatga ctgcagctga gcttgtctta atgggacata
    11521 aacagttgaa tgaaaaatct ttagtaatct tatactcaaa atctggagat acgaaagaaa
    11581 ctattaaggc agctgaatat ttaaaagaac gaaacgtgcc aaccgttggt attgtaagta
    11641 aagaagatac accgttagag gctctatcaa cttatgcaat caattaccta gacggccgcc
    11701 ctcaagaaat gccactttat ttcttaatta tgtctatcat gaatttgaat ggggaatttc
    11761 ctgaatacac tcgttttgct gatgaactga aaaacttacc aacggctctg aattcagtta
    11821 gagaacaggc tgatgaaaaa gggaagaagt atgccgaaga atttaaagaa gatccttatc
    11881 aaatttggat tggtagcggg aatttatggg gaatcaccta ctcttattca atgtgtgtgc
    11941 ttgaggaatc tcaatggttg cgtacaaagt ctgtctcatc accagagttt ttccatggta
    12001 ccctcgagtt agtcgaaaaa ggagtttgtg taacactttt gaaaactgag ggggaaacac
    12061 ggcttttaga tgctcgagtt gaaaaatttg cggtccaata tacagataaa tttaatgttt
    12121 ttgatactaa ggattatgag ctaccaggta ttagtgacga atttcgaaag tacttttgtg
    12181 ctccagttat gtgggcagcc ctaggtcgcg tcagtgttca tcttgaagat attcggaacc
    12241 actctttgga taaacgtcgt tattaccgag tagttgaata ctaaaagtag gtgattaatt
    12301 atgacagcgt atgtgattgg atttggcgat aatgttgttg attactatga gaatttgaat
    12361 caaaaatttc cagggggaaa tgcagttaat tttgctgtga atgcaaaaaa gagtggtgtt
    12421 aaagcatact atgtcggtag tctatcatct gatcctgatg gcgtgttatt acaacacgcc
    12481 atcagttctg aaggtgttga tatctctttc tcggaagtta ttgacgagcc tactgagcaa
    12541 gcacatgtaa ctattaaaga tggagatagg gagtttatag gtggcgtgcg tggctcccga
    12601 ttgacacctg ctctctcagc agaacttatt gatttgatga agaacagttc attagttcat
    12661 actagttgcc atagtcgaac agaaacaaaa attaaagaat taaaaaaata tgatgttaaa
    12721 gtttcttttg atttttctga cttagataaa tatagaacgc atgaatactt gtcagatgtt
    12781 tgtccgtcaa tttatattgc acagttttca gtcgctacag atgatcaaga ggaaattaat
    12841 cgcttaatta gtgaatgcaa ggactttgag gttccgtatc ttctttttac gcgtggtagt
    12901 aagagccctg tttttgttga cactcgagag aataagactt atgaaggatt cattcgcaaa
    12961 gttgagtcac ctcatgatac aatgggcgca ggtgatgcat actttgcagc ttttgcaagt
    13021 aattttgtct tatctaaacg tgaaaaatca atgatagaac atgttattga ttgttttcac
    13081 aaaggtgcaa accttgcata tgaaacattg atgattgatg gctcctttgg tcatgctaca
    13141 cctattaacc cttagctttg tgctttgggc ttttattgct ggaatttgta tagtagactc
    13201 atcataacta tatttttgta tgtgaaaagg caagaggcta tgatgaaaat aacactggct
    13261 aattgcatga gggagcatat cgttgttaaa gcgtcattga tagtatgaat aaatatctcg
    13321 ataatattat attgttattt acttaattta ttgattagtc aagcaaagat aatcttctgt
    13381 tacttaacga gactagtagg tacaagcact tcatgttatg tacgacccaa aaatattgct
    13441 gttgattatt tgctgaaatt attgcgagct tcaatttatt tttaaaggac ttggtaggct
    13501 gcgtgatgaa ataagacttg gaaggctgtc taaaccaata tatagtagac taaatagtaa
    13561 aaaagagcat ggtaattgtc tctttgtcaa taagtctaat ggtaataaga caagttggag
    13621 attgagaata tttttattgt gtaacctatt ttgaagcgta cttaactagc cccggccaaa
    13681 atcaaatttt ttcggggtat atcaagggtt tgcgtcaata acagaatgtt atttatctat
    13741 cacgttcctt ggtggcgtgt agagttgatg gttacgtagt agcgtatcta ccagacgcgt
    13801 aaattttctt gcggttaaga cgtgtgctcg tttgtgttta tttttgggac ctctttacat
    13861 ttgctttgat aaaaagcttt atattgagga agatattttt ttacagagtt ggaagcttca
    13921 actaagtagt atctaaaata gcgactcctt tgttttgtca gaggtgtatt ttgtgaccga
    13981 tagttaacag attgtttgcc tttccaacat aagcctgcgt attttgccaa ttttgtttga
    14041 tcttcaaatc gttcaatttg accgatttca gcgattaatc cagcggcgtg tacctttcca
    14101 atacctggaa cactagtcaa atattgatat tctggaataa cgacaacaaa ttgctcggtt
    14161 gctttatcca agtctttgat gttcttttca agcgcacgaa tttcacatac aaaaacattt
    14221 agaacgacat tgatggattc ttatgcaaga ctatctaacc gataactcac ggcaattgca
    14281 cgttgaatcg atttggcatt tttttctgga tctttaaagc gacatttcct ttttctttta
    14341 gtaagtcaca gaaagcttcc aaaggaagca cagccaattc atctagcgtg aagtcttctg
    14401 tcacaagagt aatgacagtg gcactataga gacttgtcga ttcatcgcgc atttatcgag
    14461 taagcgtatt gcatttgtac gttagatttt ccagaaagtg ttgttttgta cgagtgaagt
    14521 tgcttaatga gtcgatagcg agtgcgtgtt aaacgctgta aagccacgta cttctcttct
    14581 ttgataggag aagttgtgaa ccgttgaata cgcaataaat cagcaattct taacgcgttg
    14641 tttcgatcag tcgtatcttc ctcaaacatg cgactgaatt gtttcatccg aaaaggattt
    14701 tcaatcctca caagtgcatt gagttttttt agctctgcat cttcatggaa aaacatcgaa
    14761 ggatgaaagc tgtacatgga cgtttaattc catctcaata acgatttgac cgaagtgatt
    14821 gttttcattg aacacaagaa tcagttctcg tgtcaatgga gccctttcaa tgtcattagc
    14881 aacagaattt tcagtcaata tagatagctg atcgtcgtct gttaggaagc aaacgtctaa
    14941 tttttcagaa ctaacgtcat taccaacaaa taatttcatg gaataaaaac ctattttcga
    15001 tataaattct agaaaatgct tcgatactgt gggattccac agaaactcac tgtgtggtca
    15061 cgacctcgcc taatagaata cggtaataaa agagctaatg agttgatagt aaacacagcg
    15121 ctttggataa cagacttatg aaggtagaca aagctacagg actgattagc aaattctcgt
    15181 tgaatccttt ccatacatcc attgtactct gagaaatcac acaataacga aataaattc
//
LOCUS       DACDRN010000056        14705 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDRN010000056 DACDRN010000000
VERSION     DACDRN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.056"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            375..2114
                     /gene="yfcC"
                     /locus_tag="IWJ80_002047"
     CDS             375..2114
                     /gene="yfcC"
                     /locus_tag="IWJ80_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9461753.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2271..3455
                     /gene="iadA"
                     /locus_tag="IWJ80_002048"
     CDS             2271..3455
                     /gene="iadA"
                     /locus_tag="IWJ80_002048"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP9461754.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3566..3769
                     /locus_tag="IWJ80_002049"
     CDS             3566..3769
                     /locus_tag="IWJ80_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9461755.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3870..4532)
                     /locus_tag="IWJ80_002050"
     CDS             complement(3870..4532)
                     /locus_tag="IWJ80_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9461756.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4545..6047)
                     /locus_tag="IWJ80_002051"
     CDS             complement(4545..6047)
                     /locus_tag="IWJ80_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9461757.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6595..8274
                     /locus_tag="IWJ80_002052"
     CDS             6595..8274
                     /locus_tag="IWJ80_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9461758.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8401..8880
                     /locus_tag="IWJ80_002053"
     CDS             8401..8880
                     /locus_tag="IWJ80_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP9461759.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            9099..9779
                     /locus_tag="IWJ80_002054"
     CDS             9099..9779
                     /locus_tag="IWJ80_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP9461760.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9787..11118
                     /locus_tag="IWJ80_002055"
     CDS             9787..11118
                     /locus_tag="IWJ80_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9461761.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            11133..12095
                     /locus_tag="IWJ80_002056"
     CDS             11133..12095
                     /locus_tag="IWJ80_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP9461762.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKINVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12206..12544
                     /locus_tag="IWJ80_002057"
     CDS             12206..12544
                     /locus_tag="IWJ80_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP9461763.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12623..13330
                     /locus_tag="IWJ80_002058"
     CDS             12623..13330
                     /locus_tag="IWJ80_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP9461764.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13433..>14705)
                     /locus_tag="IWJ80_002059"
     CDS             complement(13433..>14705)
                     /locus_tag="IWJ80_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9461765.1"
                     /translation="ETDQTKKLAVFSKLKLCYLKESNRFNYLMLLINYPQTAVPKKYL
                     LEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIRLYLYFFLSDTFG
                     GNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQQAYFPKTSSEIT
                     ELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQISFRQKQEIGFL
                     FSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFFELLGESSSSFST
                     LCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQHLLQTETKEQLL
                     IHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHEKISDETETFYLD
                     TTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4727 a         2522 c         3151 g         4305 t
ORIGIN      
        1 ccaacaggat acctttttct aatagaaata aaatacacaa aaattgaagg atagataatg
       61 gatatttata agtgtttatt attttttttg caatgaaatt caatttttca atgattgtaa
      121 aatgaaaaaa acaggacgag attttctcta atctaaaaat aaaaaaagaa ttttaaggaa
      181 aaacagagaa ataaaatact taatataaat ggaaagagaa aaaaataatc atacatagga
      241 gaaaaaaaca aaatagaaaa aagtgatttt ctaataaatt attcatgtgg aaatgtcatt
      301 tttactgtat aattcatttg attatccatt ttttattcat caaaattact gaaaaaagaa
      361 aggaagaaat aaatttgaag aaaacaaatt cttcaggaaa accgaaacgc gacttcacta
      421 aattatcgac accccatacg tatgtcatta tttttggtgt agtcatcttt gcatggatac
      481 tgacttttgt cgttccagca ggaaagttca gcacgcaaga tattgaatac aaagatgcaa
      541 atggcgaaac aagtactcgt actgttttac gtcaagattc cttccgttat gcttacgagc
      601 tggataagtc gtatgtgttt gatcaattag aagaactgca agatcatcca gcagaacgtg
      661 aaaaactgga tgttccggaa aaaggattgg aaaaagtcat tgctgacgga gaaaaaaatc
      721 ttacgcagga aaaactagat gaaatctcct tgacagatga tgtgctttac gatcagtacg
      781 gcgaaaatat ctatgatact tctaaaaaat tgcataaaac agccaaaata tggggaacag
      841 atgattttgg cggctttggt ttcttaaatt ttgtttttga aggactagta tctggagata
      901 agtatggatc tgctgtaggg atcgctgcat tgatccttgt tgttggaggt gcttttggta
      961 ttattatgcg taccggagcg atcgacgcag gtatctatgc gtttatcagt aaaactaagg
     1021 gactggagcg tttagcttta ccgctattat tctttgcttt ttcattcggt ggcgctacat
     1081 tcgggatggc agaagaggta attccattct caatggtgat ggttccattt gtcattgcgt
     1141 taggatatga ctcgatcgta gcagtaaccg tgacttatgt cgcatctcaa gtcggaaatg
     1201 cgacctcttg gatgagcccg tttagcgtag cggtggctca agggattgct ggaatccccg
     1261 tgttgtctgg tgcgacattc cgattgatta tgtgggtagt tgttactgca ttagccgctg
     1321 gttatatgat gatatatgca gaaaaaatcc gtaaaaaacc agaaagttcg ttgacttaca
     1381 aatcagatga ttatttccgt tctcatatca aaaagacatc tgatgaaaat aaaaaattca
     1441 tgcttgggca taaattgatc ttattagaga tgttagttgt ccttgtctgg atcgtttggg
     1501 gcgtgactca aaaaggctat tacattccag aaatcgcttc tcaattcttt gtgatgggat
     1561 tagttgctgg aatcattgct gttcttttca aattagatgg aatgaaagtc aatgacatcg
     1621 cttcttcttt ccaatctggt gcagctgatt tagctggtac agcaatcgta gtaggtatgg
     1681 ccaaagggat tcttcttgtc ttgggaggat ctgatgcatc tgttccttca gctttgaata
     1741 cgattttgca tgggatcggg acggcgttga ccggtgttcc agctgtcatt ggtgcttggg
     1801 cgatgtacat cttccaaagt ttgttcaact tggttgttac atcaaattca ggacaagcag
     1861 ctttgactat gccaatcatg gctccactgg cagatttagt aggtgtatcc agacaagtag
     1921 cagttcttgc ttatcagctt ggttcaggtt ttatggacgc ttttacaccc gtatcagcca
     1981 gcttgattgg tgtgctaggt gttgcacgga tcgaatgggc aaaatgggca agattccaaa
     2041 tcaagatgca aggattcctg tttgttttag gaactatttt catcatgatt gccattgcaa
     2101 taggtctgca gtagtactgg agacctgtaa ataaaaaagt aatccaaaca gtagataagg
     2161 actccaagaa ccgtcatttt acggtaaaat caaaggagta aagatcctat cgtttggatt
     2221 attttttaag atcctatcat cagttatact taaagaaaga ggaaaaatgt atgaaaatca
     2281 ttcgacaaat tgaagtgttt gcaccagatt acttaggaaa aatggatgta ctgattgccg
     2341 gtgggaagat tttggctgta gaagaacagc tagaaggcgg ctacgaagga gtggaagtag
     2401 aagaactttc aggtgaggga aaagtactaa ccccgggttt cattgactgt catttccata
     2461 ttttaggtgg aggaggagaa ggcggttatc aaaatcgaac gccagaagtc acattgagcc
     2521 aattgactac agctggtgta acgacagttg tcggttgttt gggaacagac ggagaaggta
     2581 gagatatgac tgcgttgatc agcaaagcga aaggtctgga agcagaagga atctccactt
     2641 atgtatacga aggttcgtac cgattgccgg taaaaacagt aacagattct atcatcaaag
     2701 actttttgac aattgataaa attatcggaa tcggagaaat cgcagtatcc gatcaccgtt
     2761 cttctcagcc aagttttgaa gagttcgctc gtgcagtagc agatgctcgt gtaggaggca
     2821 tgttatccgg aaaagctggg atcatcaatg tccacttagg cggaggaaaa agaaagctag
     2881 agctattgac acgaatcgta gaagaaacag aaattccgat cacacaattt ttgcctactc
     2941 atgcaaatcg cacaccagaa ttatttgaag cttgcgtggc atttgccaaa cgtggtggca
     3001 cgattgactt cactgctagc gaagatcctg atttttggga gaagacagat ggtgaagttc
     3061 gcttcagcaa agcactgaaa cgattgatcg aggaagatgt ttctttagat aatttcacga
     3121 tgacctcaga tggacaagga agcttgcctt attttgatga aaacaatcat ttcctagggt
     3181 taggagtagg aagtgccaag gcattgctag tagggatcaa agaagctgtg caaaaagaaa
     3241 gtatcccttt ggaaatcgcc ttacgagcta taaccagtaa tccagcacgt atattgaaat
     3301 tggataaaaa aggaaaaatc gaaattggtg ctgatgcaga tctgtgtatc ttggataaag
     3361 aaacgcttga tattgataca gttatcgcta aaggagaaat aatggtccaa gaaaaagaag
     3421 tcaaagtatg ggggactttc gaaaaaagtt tttaatcaac agctctaact atctttttta
     3481 gaaaacgagc gttctgtttg tctgtatctg ttgttgatgt taccctgaag gtagatcaat
     3541 aaacaaacta agaggaggaa gcacaatggc agatttaaaa ggacgttcta aagatatgaa
     3601 agataaagta gtcggtgaag gaaaagaagc atacggcaaa gcaacagacg acaagagcaa
     3661 agaaacagaa gggaaagcac aatcactagg tgctgacatc aaagaaaaag ctcgaaattt
     3721 aggcgacgaa gtcaaagaca agttttctcg aaaagacgaa aaaaaataaa aaccacttat
     3781 tactaattga atcaagcaaa taaaaaatcc gaaccatatc tgcgagacag atatggttcg
     3841 gattttttat ttgatcatta tcctgttttt taaacttcga ttttttgaaa ttttttattt
     3901 tttagttcaa agacttcatc acagcttttt gctacttcaa tctcatgagt cacgatgata
     3961 atacagcgat tttgttcatg ggcgatttct tggaaaagtt tgacgatatc ttgtgaagtt
     4021 gtttcatcta gatttccagt aggctcatcc gctacgatta gttgatgttc acagcagata
     4081 gcacgaacga tagcgactcg ctgctgctga cctcccgaaa gtttggtcac ctgttttttt
     4141 gctaatattt catcaattcc tacacgagcc agtcgatcta aagcaacttg tcgataattc
     4201 tcttgcttgg tcttagtgat ttccattgct gctatgacgt tgtctagagc cgataaataa
     4261 ggaagcaagt tgtaagcttg gaaaataatc gagacatcat ttttacgata acttcttaac
     4321 ccgattttgt tcagtgaacg atcttgatag tatacctttc cttcttttgg tgcatctagt
     4381 ccagaaataa gcgaaagaaa agtcgttttt ccagaaccac ttgctcctaa aatagcgtac
     4441 atttttccag cttcgaaatt cagttctaca tctttataaa gcgcatcttg cggatcttga
     4501 taccaaaatc ccaattgttc tgtccgtaac atcaaattcc tcctttagtt taataggatt
     4561 tttcttgggt ttaatcttaa tatccctata gaagctaaaa agacagaaaa cagactgatt
     4621 ccaaatgcta ctccggctaa tagtcccaat tgtgcaggtt tgacattgat ttccaattcg
     4681 ctgacttgat cagaaacagc aaatggatta ttcgtggttc cttgttgacc atcgcgttga
     4741 ccgccttgtt cttgtctgtt acctggcatt tgtccaccgc caggagcttg ctggttttgt
     4801 gtcgtggttt ctgtctgttg gtcaagtagc tgctgtccca cggcattcgc gacgacgttt
     4861 ccgctaagcg aagcaactcc taatgcaaga atcatacaaa tagccacttc ggtaaacaat
     4921 tggagaatga ttttacttct ggattctcct aaagatagca agacgcctaa ctcatgccga
     4981 cgctcccgaa tggtaatcat aatgatcaga gtaagtatga taataccggc tacagccact
     5041 agtaaaacaa catttttaga aaaacttgcc acgttgttta aaggttccaa cattgactga
     5101 tacatggaat catttgattg taagctaaat gtatctgtgt cgatcagctt ctcagcttct
     5161 ttgagaaact catccatttt atctggatca gatagagtgt aaatagcaga atctatcgtg
     5221 ttatcgtcag atgtcccatt tagttcattt gcaaaagtgt aagaagtgta aagtgtattc
     5281 gacggattca tgaagttgaa tttcataccc atactgccgc ttgtttcgct ggattcataa
     5341 attcctttga tcgtcatttc ataagtggta tcttcatctt cagtactcgt gattgtgaaa
     5401 gtatctccga ctgccaggtc atttgtttct gctagagtag aatcaatgac tacattgttt
     5461 gtgcctttgt cgctgtcatc tattccttca ccatccgtga tttttgctgt tccatcagaa
     5521 aaattactat tttgtgcagt tgctgacaca ccactgattt gaaagtctgc ttgagtcatt
     5581 tgcggattcc cgcccattcc tccagcaggt tcaccgcctg catcagtagt ttctgaactg
     5641 tcttcacttg aatcagaact agtgatggca gttattccat cttttgccat agctgaggcg
     5701 gaactttcaa aggaataact ctttacatta tctaattcag ctattttttc cgcgtccgtt
     5761 aaggaaactg gtgtaagaga aaaactgcct ggatctggac gttcttcagt ggtagaaccg
     5821 cttgaagaag tttgttcctg ttttttaaat gaagcttctc gattggcact taaagtcact
     5881 gttgctccaa cttctttttg tgcttgattc gctgcttctt ctgcagcgct tcgaatcgtg
     5941 aggccagcta gcacaaaaat aagaatggct gtgaacacgg caaacaataa caatgaacgt
     6001 ccccatttta cttttgtact tttccatgca cgtttcataa aattcatatt ttgcatccct
     6061 cctagtagat gcatttgata atagacaacc tatcttaaag ctagcttaaa tcttatgctg
     6121 tttgcacacc atcaacatga ggttctgtat atataaaaag tcactatagt tattgcaaaa
     6181 aatgtggtaa aatgaaaaaa gtatgtttca aaccgacgaa gaagaaatga aagaggaata
     6241 gcaaaagaaa tcctagatgc tacagaggga tttttacgtg catagttgtt ctaacaattg
     6301 ctgatactag cgatttagtt tatttgttgt ataataatgg ggaacagctg aatacttata
     6361 taagtattct ggctatagcg tttcgcaaaa gatccatttt gttaatgaag tatatccaaa
     6421 ataaaatgcc catccaactg tcagaacgcg gatgtgctgg taattttcct gctgattctc
     6481 cataagttga gaaatagtga ttttgcatat gcttcattgg aatggctcat atatttagat
     6541 aaagaagaaa atcgacatct ttaaacttgg atgtcataag aagaggtgaa gtttgtgagt
     6601 accattaaaa ttatcccatt aggcggcgtt cgtgaaaatg gaaaaaacat gtatgtcgca
     6661 gaagtgggcg aggatatttt tattttagat tgtgggctaa aatatcctga aaatgagttg
     6721 ttggggatcg acgtcgtaat ccctgatttt acgtatttag aagaaaacat tgatcgtgta
     6781 gctggtgtct ttttaacaca tggacatgca gatgcgatcg gggcacttcc ttacttgttg
     6841 tcaaaaattt ctgtaccagt attcgggaca gagttgacga tcgagttagc aaaattgaat
     6901 gtcagtcgta atgaagcagc caaaggattt aaggatttcc atgtggtaga tgagcataca
     6961 gaaatagact ttggtgaaac agtagtgagc tttttccgta ccactcatac gataccagat
     7021 tcagtgggga tcaatctgaa aacaaaagaa ggtagtatcg tttatacggg agattttaaa
     7081 tttgaccaaa cggctatccc aatgtatcaa acagattatg cacgattggc ggaaatcggc
     7141 aaagaaggag tcttagcctt actaagtgaa tcggcaaatg cagaaaatcc tcagcctgta
     7201 gcttctgaag cccaaatagc agatgaagta tttgatacga tcaaatattg ggaagggcgt
     7261 atcatcgtcg cttgtgtagc aagtaatttg caacgagtgc aacaagtatt ggatgcagca
     7321 gaaaaagcac aacgaaaagt tgtcttgact ggtcaagatt tcgaacgaat tattcgcaca
     7381 gcgatgcgtt tagaaaaact tcaattacca agtgaagatc tgcttgttac gttgaaagac
     7441 atgaaaaact atgatccaga agaactgatc attttagaaa ctggccgaat gggtgaaccc
     7501 atcaagtctc tgcaaaaaat ggcaaacggt acacaccgta atgtcaagat tgaagaagga
     7561 gatttggttt atatcactac tacgccaagc atcgcgatgg aaaccatcgt ggcgaaaacg
     7621 gaagatatca tttatcgagc tggtggaaca gtgaagttga tctctgagaa tatgcgcgtt
     7681 tctggccatg ccaatcctaa tgacttgcaa ttaatgatca atttgatgaa accaacatat
     7741 tttgtgccag ttcaaggaga gtatcgtgaa ttagctgctc atgcagattt ggctcatgca
     7801 gtagggatgc catacaaaaa tatctatatt actggacgcg gagacgtgct ggaatataag
     7861 aacaagcgta tgagtgttgc aggaactaca agtgcagaaa atgtaatgat tgatggatta
     7921 ggcataggtg atatcggaaa cattgtttta cgtgatcgaa aaatcttgtc agaagacggg
     7981 attttcgtag cggttgtcac tatcagtcgg cgagaaaaga aaatcatttc aaaacctcaa
     8041 attacttcac ggggattcgt ttacgtaaaa gctagccgtg atctgatgaa agaaagcagc
     8101 aatatgattg aaacaatcgt tgagaaacat ttagaaagca atgattttga gtggagcaaa
     8161 ttaaagcaag acatccgaga tcaattaggc cgttacttat ttgaacaaac aaaacgccgc
     8221 ccagtgatct tgccagtcat tatggaagct actcaacgtc gaaataaaaa ataaacaaaa
     8281 agagtaagat gaacctctct atagagtgct cgtcttgctc tttttttgcg aaaaacaaat
     8341 aatttttgaa tacagcatcc ctcgctttat aataaacgat agaataaagg gagtgaatca
     8401 atgatgagtc aaacaaaagt cgaacgttat ttagaagaac aaaagatcag ctatcaacca
     8461 cttgatttta gtcatgtggt agaagaatct ttcacagaag aactacaaaa aagagggatt
     8521 gatccagctt tgatatgtaa aactctcgta gtgaaaggaa acaaaactgg ggtgatcatc
     8581 gcattgattc ctttgaacga tcacttagat tacaaaaaaa cgagacaagt gacgaaagat
     8641 cgtaaagttg gttttcctgg aatggatttt gtattaatgc atacaggata tccccacgga
     8701 gcaaacaccc ctattggaat aaaactcgca catcctgatt attgtttttt agcagacagt
     8761 tctttaaaag aaaaagaaga agtaattgtg tcaagcggtg aaatcgggag aagccttcgg
     8821 ataaaagtag ccgatttaga aaagatcgtc catcctattt acgctgattt aattagttaa
     8881 gggacgagaa gagaagctaa gcagagcaga gacctcaaac ttgagattat atctgcttgg
     8941 tacttggtgc atcctgtatt atctgttaca attgctttgt acatactgta ctatcaatcc
     9001 aatgaaaata tatttagcag ctatattttt tcattgatgt gcaagtgatg aaatcgtttt
     9061 taataaaaaa gatacagaga aaacgcgagg tgtaaaatat gaaaattacg tatcatggac
     9121 attcctgttt aacaattgaa atggaaaatg gccaacgcgt gatgattgac ccgtttatta
     9181 caggaaatcc attgacagac ttaactgcag aagaagtaaa gacagattgg ttgctgatta
     9241 ctcatggtca tagtgaccac ataggagata tgattcctat tgcaaaagaa aatcaagcca
     9301 caattatcag tattgttgag attgctgatt atgcgaacag cagaggagta gattcattcg
     9361 gtatgaatat cggtggaaaa catgcgtttc cattcggcgt ggtaaaattc gtacatgcac
     9421 agcatagttc aagctatgaa gtagacggta tcgttcagta tatgggcgag cctagcggaa
     9481 ttattattca agcagaagga aaaacgattt atcatgcagg ggatactgct tatttcagtg
     9541 atctaggatt gcttgcagaa gaattcgata ttgatgtcgc tttcttgccg attggcgata
     9601 actatacgat ggggccagaa gatgcagcaa aggctgcgca aacaattcgt gccaagaaag
     9661 tagttccaat ccattacaat actttccctg tcattgaaca agatcccgaa tcttttgtag
     9721 agttactgga agatagtgaa ggaaaaataa tgagcgtctc ggaaacgctg gaagtgtagg
     9781 cagaaaatgg cgacaaaaca tgatttgatt ttagaatata ttgaaagttt acctgttggc
     9841 aatcgaattt ctgtaagaag catcgcaaaa gaattgaatg tcagtgaagg aacagcgtac
     9901 cgtgcaatca aagatgcaga aaatgtcgga ctagtttcaa caattcaacg agtagggacc
     9961 atccgtatcg aacgcaaatt aaaaaaacat attgaacgtt tgacatttgg cgaggtcgtc
    10021 aggatcattg aaggggacgt tttaggcggg gcggcaggat tagacaaggt cttgaataaa
    10081 ttcgtcatcg gcgcgatgac taaacatgcc atggaacgct atattacgcc aggatcacta
    10141 atgatcgtcg gcaaccgggc ggaagtccaa aaattagcat tggaagatgg cgccgcagta
    10201 ttgatcacag gaggattcaa tacgactgct gagattgcaa agctagctga tgatcttgaa
    10261 atgccgatct tacgcacctc ttatgataca tttacagtcg caaccatgat caaccgagcg
    10321 ttaagcgacc aattgatcaa aaaagatatc atgcttgtca gtgatatcta tatgccagct
    10381 gaaaagactc attatttgca gcagacagat acgatttctg attatcagcg tttgtcagaa
    10441 gaaacgcagc attcgcgttt tccagtcgtc aaccgccatc atcgtttgat ggggattgtt
    10501 acggcaaaag atgttttagg gaaatcgcca aaccagatca tcgatcgggt gatgacgaaa
    10561 gaaccaatca gtgtaaagaa aacaatgagt attgcctctg ttagccatca aatgatctgg
    10621 gatgggctag aagtcatgcc ggtcgtttcc gatgacttga cattggaagg cttgattacc
    10681 cggcaagatg taatgaaagc gatgcagctt gtccagcgac aaccgcagat tgcagatacg
    10741 atttcggatc agatatctgg aaatatccat acgattgata ctgaccgtga aggaaatcgt
    10801 ttggaaaacc ctaagttcaa attcgtagta gcaccacaaa tggtcaatgg cgttggaacc
    10861 atttcttttg gcgtacttag cgaaatcatt tctgatgtcg cacaaaaaac aatggtgatg
    10921 aaccaaaaac gaaatatctt gattgaacaa gtgaacctac attatttgcg gctgatccaa
    10981 ttggaaagcg aattagacat tcgtccacgt gtattggaaa tcggcagacg ttccgctaaa
    11041 ctggatatcg aagtgtttat tgaaaacacc atcgtttcca aagcaatcgt tgtttgtcaa
    11101 gtaatggaac gttcttagaa agcaggagtg aaatgacagt acagcaagaa atagtagata
    11161 caataaaaaa atacgataaa atcattatcc acaggcattt gcgccctgat cctgatgcat
    11221 taggatcaca agtaggatta gccgaactat tacgggaaag tttcccagaa aaagaaatac
    11281 gtcaagtagg tgaaacgatt gaagggcttc gctttttagc agaaatgcaa gaagttgaag
    11341 atgtcttcta ccaaggagcg ctagtcatcg tgactgatac agctaattca cccagaatca
    11401 gtgatcaaag atatcagttg gcagaaaaac tgatcaaaat cgaccatcat cccaatgatg
    11461 acccttatgg agatcttgtg tgggtaaata caaaagcaag tagctgtagt gagatgattg
    11521 cagaatttgc tttgatgttt cctgaatctt taaagataaa cgttaaggct gcacggttac
    11581 tttatgcagg aatcgtcgga gatacagggc gctttttgta tccctccacg acgtcccgta
    11641 cattagagat tgcttcgata ttgagaaatt atccatttga tgcaagtgct ttgaacagag
    11701 agattgaaca aatgccgatg aaagtcgcta aactttccgg ttatctttat caaaatatcc
    11761 aggtggatga aaatggtgca ggaagaataa tccttccgca agaaattctt gataaatatg
    11821 gaatcgatga ttcagagaca gcttcgatcg tttcacttcc tggagtgatt gacgaagtgc
    11881 tggcttgggg gatttttgtt cagcagcctt cagggtatta ccgcgtacgt ttacgttcta
    11941 aggggccgat catcaatgaa ttggctaaac gccatcatgg aggaggccat ccattagcaa
    12001 gtggtgcgaa tgcaaaagat ttagcagaag tagaggaaat ctataaagag atccaagata
    12061 tatgccaatc gtataagagc agaaaaacag aatgatactt cgttcgaaaa cgtgcctttt
    12121 gtagtaaaaa tcacaaatgt atcttttcaa gatcttcttt ttccgttaca atagtgctca
    12181 ctaaagattt taaaggagaa agaagatgac attaccaaat tgcccagaat gtggctcaga
    12241 gtacacatat gaagatcgag gcttgttgat ttgcccagaa tgcgggaacg aatggaacaa
    12301 agaagaacaa gaaactgtca tagaagacgg attagtggta ttagatgcaa atggaaaccg
    12361 tctgttagag ggcgatagcg tgacagtgat taaagacttg aaagtaaaag gtgcgtcagg
    12421 tgccattaaa caaggaacaa aagtcaagaa catccgttta gtagaaggcg atcataatat
    12481 cgattgcaag gtagaaggat ttggaccgat gaaactgaaa tcggaatttg tgaaaaagaa
    12541 ttaaagaata ttcaaggtct tttcacattc ttttcctact actaatgata aaataaagta
    12601 aacgaaaagg agtgaaacga aaatgtatgg ttatggaatg tatggttttt tagatccgac
    12661 atttcttttg gtcattcttg gattagttat ttccggaatt gcgtcagcgt acgtcaatag
    12721 tactttccga aaatacgatc aagtgagaag caaaaacaat gtgactggta cacaagcagc
    12781 acaatatata ttacaaagtc aacagataaa taatgtcggt gttcaacaaa ttgccggaga
    12841 tctcacagat aactacaatt caggtaataa gatgttgagt ttgtctcaag caactgcaca
    12901 gtctacttca gttgctgcga taggtgtagc agctcatgaa tgcggacatg cggtacaaga
    12961 tgcttcgggt tatgtcccat tgaagattcg tgcagcactt gttccagcag ctaactttgg
    13021 ttcgatgatt tcgttcccct tgattatggt aggtgtattg cttagctgga actctacgtt
    13081 aatcaatatc gggattttcg ctttttcttt agcattactt ttccaattgg tgacattacc
    13141 agtcgaattt aacgcgtctc ggcgagcaat ccaaattttg agtgaaggcg gactactgac
    13201 ggaagaagaa gtaccgatgg caagacacgt attatttgca gcagcactga cttatgttgc
    13261 agcagcgctt tcgacattcc tgcagctgtt gcgcttgatc ttattgttcg gcggcaaccg
    13321 aagagattaa aataaagaag ttgtgaaatc agcgctttga tctgagctaa taccgagagt
    13381 tagctgattg aataccattt atttggaata agaggatgtg acaagaattt tgtcacatcc
    13441 tctttgtttt gttctcataa ccttttttca tatacagttc atgaaccgct tttattaact
    13501 tgccaaataa cactgtattg tgcgttgtat ccagatagaa agtctccgtt tcatctgaaa
    13561 ttttctcatg ctgatctgta atgatcaaat cagcatgttc tgaatatgcg gtaattttaa
    13621 cagcatcttt gcgaaataac tgctgcaaat ggctttgaag atgataattt cctgtaaact
    13681 gtttggttat ctgcaaatgg atgagtaact gctctttcgt ttctgtttgt agtaagtgtt
    13741 ggatcaattg actaaggtat tgactgattt ggtttctttc cccatttttt gaaaaaattg
    13801 tatcgatttt ttctcttaat ttcttttcct gtataaaaga actagaatga ttggacgtgg
    13861 cttctggaaa gcaaagtgta gaaaaactac tgctggattc acccaacagt tcgaaaaaaa
    13921 caactgccaa cacaagaaaa taaaaataaa ttggtatttt attttctaaa tggttgagag
    13981 gaaactgttg agttaagtaa gtcatcaatt gaccgacttg tcgacaaata aggtttttgc
    14041 ttgtggaaaa taaaaaacca atctcctgtt tttgtcgaaa agagatttga tttggaaaaa
    14101 aacaatagaa aaaataatta aagtaggctc tctctgtttc tgtctcttca caagggcgac
    14161 tcactgaaaa agaagtaaag tattctgaat aatcatgtgt atgctgcaat aagtccatca
    14221 attcagtgat ttcagaagaa gtttttggaa aatatgcctg ttgtttgttt cgtaatatga
    14281 ataaagttaa cagcaagtag atattttttt cttgttttga aattttttct atttgctcat
    14341 ctgtactgtt catttttagt gcatttaatt caagtgagga aaatggccaa tcgttacctc
    14401 caaatgtatc tgaaagaaaa aagtaaagat agagacggat agataactca tttccttcaa
    14461 gagtacagca attttttaca gataggatcc gtaaatggaa tttttttaag gaatgattga
    14521 gctgttttgt cagtttttcg agataggagg gcgatatata aagttgttca agtaaatatt
    14581 tttttggtac agcagtttgt ggataattga ttagtaacat caaatagtta aaacgattgg
    14641 attcttttag gtagcataat tttaacttag aaaaaacagc taacttttta gtttgatcgg
    14701 tttct
//
LOCUS       DACDRN010000057        14075 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDRN010000057 DACDRN010000000
VERSION     DACDRN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.057"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..587)
                     /locus_tag="IWJ80_002060"
     CDS             complement(<1..587)
                     /locus_tag="IWJ80_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461766.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVF"
     gene            complement(735..1277)
                     /locus_tag="IWJ80_002061"
     CDS             complement(735..1277)
                     /locus_tag="IWJ80_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP9461767.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            1412..1801
                     /locus_tag="IWJ80_002062"
     CDS             1412..1801
                     /locus_tag="IWJ80_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461768.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1805..2569
                     /locus_tag="IWJ80_002063"
     CDS             1805..2569
                     /locus_tag="IWJ80_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9461769.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2698..3645
                     /locus_tag="IWJ80_002064"
     CDS             2698..3645
                     /locus_tag="IWJ80_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9461770.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3775..4515
                     /locus_tag="IWJ80_002065"
     CDS             3775..4515
                     /locus_tag="IWJ80_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461771.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4806..5474)
                     /locus_tag="IWJ80_002066"
     CDS             complement(4806..5474)
                     /locus_tag="IWJ80_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9461772.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            5608..5895
                     /locus_tag="IWJ80_002067"
     CDS             5608..5895
                     /locus_tag="IWJ80_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461773.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            6311..7849
                     /locus_tag="IWJ80_002068"
     CDS             6311..7849
                     /locus_tag="IWJ80_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP9461774.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7846..8811
                     /locus_tag="IWJ80_002069"
     CDS             7846..8811
                     /locus_tag="IWJ80_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9461775.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8798..10120
                     /locus_tag="IWJ80_002070"
     CDS             8798..10120
                     /locus_tag="IWJ80_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9461776.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      10183..10287
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10361..11104
                     /locus_tag="IWJ80_002071"
     CDS             10361..11104
                     /locus_tag="IWJ80_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9461777.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            11119..12111
                     /locus_tag="IWJ80_002072"
     CDS             11119..12111
                     /locus_tag="IWJ80_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9461778.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            12123..12860
                     /locus_tag="IWJ80_002073"
     CDS             12123..12860
                     /locus_tag="IWJ80_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9461779.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12850..13572
                     /locus_tag="IWJ80_002074"
     CDS             12850..13572
                     /locus_tag="IWJ80_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9461780.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(13656..>14075)
                     /locus_tag="IWJ80_002075"
     CDS             complement(13656..>14075)
                     /locus_tag="IWJ80_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP9461781.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         4714 a         2387 c         2911 g         4063 t
ORIGIN      
        1 ataaatacac ctagactgac gatgaagagc atatccaaca gatttcctgc gacttggatc
       61 gtcgaagggt tggtcagtgg cattaaagca aggacaaaaa aggaaaacaa gctgtaaata
      121 aaacaggtga caggaactaa tcgaaagaag agcggacatt tcaattggct aaaaatgagt
      181 gaaaaaataa aataagtaaa aatggataaa atcacgacta aaccattcat gataaagaag
      241 aaaagcgtat tgtgattaaa cgaataaaaa agaattgcta aaaaagaaaa cgttttaaat
      301 gcaatcaata cgcttgaagc ttcgtttaac gtaccaggat gtcgatgaaa aaaataggca
      361 ccgaaataaa cagaaaacag taagccgcaa agagaagaca aaatcaaaaa aaacggattt
      421 tcttcggctt ctgtgatatc agaatgatcc aaaagcaacc agcctaatgc actagaaggc
      481 gcatgtgagg tcttaaggta tgaataacat agaaaaaata agatcgtcaa agttgcaaaa
      541 aaagagccta aatgtaaaaa attagtggtt ctacgttgaa tttccataat atctccaaat
      601 taaattgata agtatataaa gtataataac aaattgatga gtatatgtcg atgtttttta
      661 atcaaaataa taaataaaaa ctgtctaaca tttaaaattt aatttagata ttagacagtt
      721 tttaagtttg tgagttattt tccttgtcgt tttctcactt ttttagtcca gaaaccacct
      781 gaaatatatt ttggctcata agaaatgata aaagcacgct cttcgatttc tttgatcagg
      841 cgatacagtt cacgttcgct ttttctagga gaaagtattt cgagaactag ccgctcacct
      901 tccagcccca ttgcagaagt ctgtgtcaca ccataaccgt gttctctcag tatacccggc
      961 aaacttttat cttctgcgct atttgctggc aaaatcgttg tgaccatgat gtatcctaat
     1021 gccaatttgt cttcaatcaa aattccgaca ctgataccta ctgcgtaacc taacgcatag
     1081 acaaataaat tcagtggatt atctaaacga tctaaaacaa gggataagcc gacgatataa
     1141 atcgtgatct cgaccatact gacaagtgga gcaatcagtc gatagccttt cattgtcaac
     1201 atgaaacgta acgtgttcag agtgatataa gcaaaattaa tgaggaagat catccccaac
     1261 atttttggat caatcatttg taaaactcct ttcatttata taacaacaat atcaaaaaaa
     1321 atcgtaagtg aagaaaaatt gaaactttat acatacaaaa aaatgataaa atgattataa
     1381 tagaattata aagataggtt aggtgattaa aatgaaaacg gtcaatcgtg tgttttgtgc
     1441 cagccacgtg ctaagtctcc ttctcagtat atacttaggt gtctattcga ttcaacagcc
     1501 aattgaggta gagaacattg tttttcagat gtcgctgaca gatggtttaa ttttaagcgt
     1561 ttttttcttt ttgatactgt ttattggtaa tattttcggt agcgttgcca gttttttgaa
     1621 tttttctatt tatccactcc tttcgctagc attaggagtg gtgggacttt gttctctgtg
     1681 cttatttccc gagccattca gtcctgcatt tatattattt ggtacactga acctgttcca
     1741 gacaactgtt ggagcatggt tgctatggcg atcaggaaat ttaatgaaaa taggtgagta
     1801 acaattgaat ccaattatat tgaaacgttc tgtccatcat attccagtgt tagaagttgt
     1861 tctgaaagaa ttaagagagg aacgtattcc cgttgtcatc tattatcacg gatggcagac
     1921 aagtaaggag ttagttttga cgcaaggaag aaaaattgcg cagcagggca ttcgtgtgat
     1981 cctgccagat gcgatgaatc atggggagcg caaacagccg gtttcttcta ttccatccta
     2041 tacattttgg gatagtatct acggaaatct gtttgaattt gacacactta tcaagcattt
     2101 agaaaaaaga ggtcttctac atgatcagct tgcagttggc ggggtttcga tggggggaat
     2161 gacaacatgc gccttgttgg ctaaacatcc tcatatagta gcgggcgtgt gtttgatggg
     2221 ttcccctgca cctttggaat atggaaaaga aatcgtaagg cgcgcatcgg aatataatta
     2281 tgtcgtacca aaagactatt ttgatttgat ttcatgggtt aagagctatg atttgtcatt
     2341 gcagccggaa aagttagctg gacgtcctct gttcttttgg catggacaag ctgacgaaaa
     2401 aatcccttat atacaaacgg aacgatttgt agaaaaaaat cggcataagt cttacggaaa
     2461 agaaattgtt tttcgttcct caaaaacaca gcgacatatg gtacaaatac cactaatgga
     2521 agaagcttcc acttttttag caaaaaattt gataaaaaag tataaataat tgaaacggaa
     2581 aatcactcat gacgatagag gattttccgt tttttatttt gggctataat aaactctgtt
     2641 aggaaaattt ggtataattg atagtaagaa tgacaacatg ttagaaagga agagagcatg
     2701 cccactttag cagatgttgc aaaacgtgcc aatgtttcaa aaatgacggt ctctcgagtc
     2761 atcaatcatc cggaacaggt gactgacgaa ttgaaagagt tagttttctc agcaatggct
     2821 gaactggatt accgtccaaa tattgctgca aaagcacttg tcacgaatcg gtcgcagatc
     2881 atcaaattat ttatactgga agaaatcgac acaactgagc cctattacat gaatttgctg
     2941 atgggaatcg ctaaacggat cggtaaagcg cattattctc tccaattagt tacaaatgat
     3001 gccttcgacg tcggttcttg tgatggatac atcatcacag gtgtcagaaa aaaagacttc
     3061 gattggatcg accgtttgga aaaaccagtt gtcttgtttg gggaaaattc tgaaggatat
     3121 gattatgtag acagcgataa tgaatatggt acagcaaaag caacgcaata tgccttagaa
     3181 agagattatc aaacgattat ttatatcggt atcgaagtgg acgagccttt tgaaatttca
     3241 agggaaactg gttatttatc tgtgatgaag gaagttgatc ttcctagtaa aatcttgcgg
     3301 tttccaaatc attcgacaga agtagaaaga tatatgacag aaaactgggc agattttaca
     3361 gataaaactt gttttgtatg tagttcagat cggctagcac taggagcagt aagagggatt
     3421 ataaatgctg gaggacactt gcccgaagaa tttggtgtga ttggatttga tggggtattt
     3481 ttagaccaaa tcagttcacc taaattgaca acgatcaagc aagatattat acgtatgggt
     3541 gaagtatgtg gagaaatgtt gttaaaaaaa atagaagaaa aaggggcgag tcaaggttat
     3601 cgtcgttttt ttcctgaact agttatcaga gagacaacga attaagtctt attactatat
     3661 atatatttat gaaattcatc gctattttct taaaataatc agcgcattgc tactgaaaac
     3721 aatgaatctg tgctattatt gagaaaagtt ccatttttga gaaggatggt ttgaatgaaa
     3781 gaaatcagac gaaaacattt ccgttttcct gcgtacgatg atgagttagg cgtaaaatta
     3841 accaacgaca agaagcgagt attatttgac ggacaagaag atcttttgac tgaacagaat
     3901 cagtccccgt ttgaacagat gaaagaattt catttcacga acacgaacac cgagaaaatc
     3961 cgtcgcaagc gtcgtgcaac aaatggattg aagtcagctc cgcgtaagga aaatttggaa
     4021 aagcataaag gaaaattacc ggattatacc ggccattacc aatcagaaat cacatcgaca
     4081 ggacgaaaaa aactttttgg tgattcctct cgttctgtca gacaaagtga aacaaggaca
     4141 gagtccaagc atactacacg aaacgcatct gttgacacga aggtgcggga gcgttcttat
     4201 tttgttccta aatatgtacc cgcatcgatt atcccagacc ctagagaaag caaaatttct
     4261 gaggatcatt taatcgaatc gatgaaaaaa acggatgact cttatctgtt gttcgataca
     4321 gaaccgacgc cgtatcaaga aaaaagaaat ggtgagccgt ctgtacgaac attcaatcga
     4381 acaaaaacgt tagaggaaac aaagcagtca gaagaaaaaa aatcgaaaaa aagccatggt
     4441 atcttagaac gctcattaca aggattgatc gatgatcaag gaaactcttt gggagaaaat
     4501 agctatttta aataagtgat atacaaattt atattcggct acagttggaa aggaaacgct
     4561 gcggcgtttc cttattttat actctatccg aaagtttata tcgtatcctc aaattttttt
     4621 gagtttaagc gaattttttc tcccaataag taagaaaata atggcaaaat aaaaaaacaa
     4681 actattttcg cagatcgttt gcaaaaatag tttatttttt tataaagaag attttactga
     4741 ataatatcat tttagtttac ataatgatat ttctgtaaat taaataaagt agtgaaaaat
     4801 aagaatcagt gctctaactg ttcaagagga tcaagtggtt caaatgtttc tgtagtctgt
     4861 tttgcttcca attggggcaa aacagatcca atttccagat acttcacttt attcaatgta
     4921 taagatagaa tagaacagct aagtaatgta tacagaaaat gtgtgagatc caaataaagc
     4981 aaggagacaa ctaaaatacc aaaagtaatc aagaaaagac ttttgccaat cttaatatga
     5041 aaatattttt caatggcttg ataaatgata tctgttccgc ctgtgcttcc gccgaatcga
     5101 aagacgagac cgcaaccaat gccagaaaga atacctgtca tcaaactatt gatcagtaga
     5161 ctatctacag caaaatgtat atgaaggctc tcccataaat gtaaaaagaa aactagcgca
     5221 aatgttccga cgatcgtccg gagaaaagcg tgttttccaa aaagaaacaa tgagataagc
     5281 aataaaggta aattaaaaag cagtgaagtg acagaaggag cgaatccaaa aagattatgc
     5341 aaaagcaaag caataccagt aaccccacca tctgccagct tagatggaat attgattcct
     5401 gtcacagcta atccgtagat tcctgcgcct aaaatgattc cgcacaattc tgtaaataaa
     5461 tttaattttt tcatttctag cctcttttct tttgaaaatt ttcagcaact tctttattat
     5521 actgacttat actcttactt caagaggaaa atgaaaaaaa attatttttg gctgtttctt
     5581 tttatttatc aaataaggag tggaaaaatg cgagcagcag acttattgac aattcttaca
     5641 aaatcaccca aaaaaacaga gtatcaagga gtttttttgg ctcaagaaca aaaactggtg
     5701 cctgtgacaa tggtccagat aaataaagca aatcaattta tctttttatt tgaacacggc
     5761 aaagcaccac ttgctatgaa agacatcttt ctttttttga tgaagaaccg tgagaaagct
     5821 gtttattatc agtcaaaaga agaactcatt cctctttatg gagtgaaaga gatagaaaat
     5881 aaagtggtga tataagtaaa aataagaaaa caagacgttt atgatgtcaa aacaaccatt
     5941 ttttacattt ttggcaaaaa agtgtcatga tacagtataa tattcgttga acaggccaat
     6001 aaaggtgttc tcccccacga gatttctact ttattggctg tttcttttta ttaaaataaa
     6061 agttgtgtgt gtaaaaaaga tatagctgat tccaagtgac aatttcgtat agatttttat
     6121 ggtattcgta gtgaaaaaac gagaaccaaa aagttgatcc gactttgttg cttggattct
     6181 ttttttgtca gcatatttag aggtattttt ttcttttttt tgttcgtgct gtaaggttaa
     6241 ggcaagacaa gttagttggg aaaaaggaaa gaaatgttta tacttaaaga ataacagaaa
     6301 ggaaggtcgc atgaataatt ttatacaaac attaagtgaa agaaaaggcg agttgctgga
     6361 agctgcattt caacatctgt ccatttctct catttctttg ctgattgcag cactgattgc
     6421 tattccttta gcgatttggg cagccaatca taaaaaaatg gcagaagtct tattgcagat
     6481 cacgggtgtg ctccaaacga ttccttcact tgccttatta ggtttactta tcccatttgt
     6541 agggatcgga actgttccag cattgattgc acttgttatt tacgctttgc ttccgatttt
     6601 ccaaaacacg tatattggtc tagctgaaat tgacccgtct attgaagaag cagcggtagc
     6661 atttggtatg tccagaatga gacgcttgct gaaagtagag cttcccatcg cattacctgt
     6721 cattatctca ggtatccgaa ctgccttagt tttaatcatc ggtacggcta ctttggcagc
     6781 attgatcgga gcaggtggat tagggacatt tattttgtta ggtattgacc gaaatacacc
     6841 tgttttgaca ttgattggtg cgatcagttc tgccttgctt gcaattatat tcagcggact
     6901 gattcgctgg ttgcagcata agaaacctcg atattccatc attactttag cagcagtggt
     6961 gtgtttattt ggcggcgttt ctttatatca gtctgccttg tttaaaaatg aaaccatcac
     7021 gattgctgga aaattaggag cggaacctga aatcctgatc caaatgtata aagaattgat
     7081 tgaagacaac acaaaaacag aagttgaatt aaaaccgaat tttggcaaga cgagtttttt
     7141 gttcaatgca ctggatcaag gtcaaatcga tatctatcct gagttcacag gtactgtcct
     7201 agaaagcttg gtgaaggtac ctgaagcaat gaaaagtgct gatttgacaa aagaaaaaac
     7261 atatgacgaa gcagaagaat tattgaataa gcaattcgat atgacattat taaaaccaat
     7321 ggcttaccaa aatacgtatg ccttggcagt caagagatct tttgcgaacg aacatcaact
     7381 taaaaagatt tccgatttgg cagctatctc tgatcagatc aaagcgggat tcacactgga
     7441 atttattgat cgttcagatg gatataaagg aatccaagaa gcatatggct tgaatttttc
     7501 aagtgtacaa agcatggagc caagtttgcg ttaccaagca atcaataatg gagatgtcaa
     7561 tattgtggat gcctattcca cagatagcca gctcaaggag tacgatctag tgactctaga
     7621 agatgatcga gggttattcc cagcttatca aggcgcaccg ctgatgaaaa aagattttgc
     7681 taaagaacat cctgaaatcg ttaaagcact gaataaatta agcggaaaaa taacagagga
     7741 tcaaatgatt gatatgaatt accaagtcaa tgtcgaaaag aaaaagccga cagaagtagc
     7801 acgaatcttc ctgaaaaatg aaggattagt aaaggaggaa gatcagtgac taaattaatc
     7861 gagttcaaac atgtgcaaaa aaagtatgat ggaaagtatg tcatcgatga cctaaatctg
     7921 gcaatcaaaa aaggagaaat ctttgttttg gttggtcctt ccggtagtgg aaaaacaact
     7981 actttgaaaa tgatcaatgg attatctaaa ccatcagcag gagacatcta cttcaaagga
     8041 aaatcgttaa atgaatataa tctgcagaaa atgcggtgga atatgggata tgtgctgcag
     8101 caaatcgctt tgttcccgac catgacagtc aagcaaaata ttgaagtcat ccctgaaatg
     8161 ttaggttggg aaaagcagaa aagagcagac cgtgtagatg aacttcttca aaaagttggc
     8221 ttatctcctg atatttatcg agatcgtatg cctagagagt tgtctggagg agaacagcaa
     8281 cggatcggga tcatccgagc tattgctgca agcccagatg tgattttgat ggacgagcca
     8341 tttagcgcat tagatccgat ttcaagaaac tcattacaag aattggtttt gtccttacac
     8401 gaagaattgg gcacgaccat cgttttcgtt acgcacaata tggaagaagc aatcaagcta
     8461 ggtgatcgta ttgcatttat gaaggatgga gaaattatcc aatgtgacac accagagcag
     8521 ttattgatga atcctaaaaa cgactacgtt cggcatttct ttgatgaacc gaaacagacg
     8581 aaagagtggc gagtagaaga tttagtcgtc aatggatatt tcttaaatga aataccagaa
     8641 aatgctcgtc aacaagtttg ctttgatacg cctatgaaag aagtatattc gcttctttcg
     8701 gtgtatccaa gcattgtgat agtagaaaag caaagaagta ttgggtcgct gacaagtaaa
     8761 gaaatctttg cttttttaag tcgagaggag gaaggaaatg aaaacatatg atgcaatcat
     8821 catcggttca ggtgtgagcg gcttaagtgc tgcttatgga ttgaaagaag caggtaaaac
     8881 agtactagtg gtcgaagaag atctatgggg cgggacttgt cctaaccgtg gatgtgatcc
     8941 taaaaaagtc ttgttaagtg cagtagaggc acgtaatcgg gtaaaacaat tgagtggaaa
     9001 aggttttaac gagatcccaa ctgccaactg ggaagaactt caaaaattca aacgaacatt
     9061 tacggatcca gtcccagaaa gccgaaaaaa acaattagca gaagctgaaa tcgatcatct
     9121 ttcaggaacc gctcgttttt tggatgattc atctattgaa gtcaacgaag aagtcttcca
     9181 tgcggattac ttagttttgg caaccgggca aagacctacg atcttaccag tggagggaaa
     9241 ggaatacttg aaaactagtg cagacttttt atcattgcct gtactgccta aagaaatcat
     9301 ttttatcggt ggtggttata ttgcttttga gctagctacg attgccaatg ctgcagggag
     9361 caaagtaacc attgtccatc ataatcaacg gccattaaaa gaatttgagg cgtctttggt
     9421 agaggaagcc gtccatcaaa tggaagcatc tggcattcag tttgcttttg gtgttgaaac
     9481 ccaaaagatc atttcagaag gtactcgcta tcgtttagtc ggaaaagaga cggaattagt
     9541 agcagatatg attttttgtg cgactgggag acagccgaat acagaatctt tagcacttga
     9601 gcaagcgaat atcgtctttg ataaacacgg aattgcagtc aatgattatc tgcaaacgag
     9661 caatccaaaa atttttgcgt gtggagatat tgtttcaagg aaaaccccaa aattgacacc
     9721 agtcgccact tttgaaggaa attatgtagc taagcggata acggatgcaa caagcgaacc
     9781 tatcaaatat ccgattatac ctacgatcgt ctacgccagt cctaaacttg ctgaagtagg
     9841 agttacaaaa agtcacgcct cttcgtcaga tcaagtggta gagatggatc ttaccagttg
     9901 gttcacctat catcgtgtca atgaaccagt tgctaaggct gaattgacct ttgaccagca
     9961 aaattatttg attggtgctg cagtgattag cgagcaagca gacgaattga tcgatgattt
    10021 gacactcgtc atcaatcaaa aattgactaa aaaagagttg gatagctata tcatggggta
    10081 tccgacattg gcaagtgatc tctcctattt attgaaataa tcagcatgat aactaaagcg
    10141 cttgttgctt tttccttctt gtgctacact ttaaaaaatg aatacctgtt tctggggatg
    10201 tcgtagacga catgagagta agttgtgaac ttagacccat tgaacctgtc agttaatact
    10261 ggtgaaggga gaaacatagc agacgcttga agcgggttac tatgtcgtgc ccatttcaag
    10321 cgttttttgt tattcatatt agaagaaagg aggtaacaaa atgaagaaaa atctttatcc
    10381 agcagtcagt atggcattca ttttaattat ttggcagatt gttgtcatag tttggaaaat
    10441 acctgccttt attttacccg gacctgctgc tgtattttct gcattagcgg cagatgcaca
    10501 agtattactt tcccattctg tcacgacatt aaatgaagca gtgcttggtt tggtaatagc
    10561 tagcgttctt tcttttatta ctgccttggc aatggattat tggaagtttt tagaaatgtc
    10621 tttttatccg ttactagtga tttcacaaat gttgccaatc atggtgttag ggccattact
    10681 taccctatgg tttggtttcg gtatgcttcc aaaagtgatc cttgttgtct tgataagcta
    10741 ttttccgatc gtagtttctt tttcggatac attgaggaaa gtatcaaaag agcaaatcat
    10801 ctttttgaaa acgatggggg cagatacagg gaagatttat cgaatatata aaataccagc
    10861 gggtttatcc ggcttctttt ccggtttaaa agtggctgct acttattgtg tcagcggagc
    10921 agtcgttgga gaatggctaa gcgctcaagc aggcttaggg tattacatga tacgcgtaaa
    10981 aaacagctac cagatcgata aagtttttgc tgcgatcatt tgtgtcattc tattgagttt
    11041 attgctgaat ggcgcttgtt cattattgaa aaaagggtat tatcgtatgc tttatatttt
    11101 ttaagagaag gggatcaaat gaaaaaatta tggcttttat taccgctact gttgctttta
    11161 tccgcttgtg gcactgcaaa agaaacaagt agcaaacaag aaataaagga tttgaaaaaa
    11221 gtgacacttg ttttggatta tgtaccgaat actaatcata ctggcatcta cctagcaaaa
    11281 gaaaaaggct attataaaga ggctggttta aatgtccaaa tcatcgaacc aggagataat
    11341 tcgacaagta ttggtctagt aggtgcggac aaagcacaat ttggtgtaag ttatcaagaa
    11401 gatgtcactt atgctcacgc agatggacag aatataccag tcaaagcaat tgctacagtg
    11461 atcaaacata atacttctgg attcgccacc ctatcagaca gtaacatcca ttcaccaaaa
    11521 gattttgaag gaaaaacata tgcaggttgg caaagtccaa gtgaagaagc tgtcttaaaa
    11581 gctgtgatgg aaaaagacgg cggggacttt tcaaaactga caatggtagg aagcaatgga
    11641 gaagggccag aaagcttagg aaaatcctca gatatccaat ggtactttga aggttgggat
    11701 atgatcaaag caaaagaagc gggtattgaa ctaaactata tcccattgaa agaattagat
    11761 gaacggttag attactatac tccggtaatt attacgaatg atcaactgat caaaagtgat
    11821 ccagaattgg tacagtcttt tatggatgcc acgaaaaaag gctatcagga agctattaag
    11881 gatccaaatg acagtgctaa actgttgcaa aaatatgcaa aagaaaatga tcggacgttt
    11941 ttagaagaat cacaagcatt cttgtcaaaa aattatacgg atgatcctga gaattgggga
    12001 ttgatggaag aaaaagtttg gcgcaattat acagctttta tgcaggaaaa cggattgatc
    12061 aagcaagatg taccttcgca agaactattc actaatcagt ttataaaata ggagtgatag
    12121 atatgtcaat ccaaatcagc cacttgaatg tagtcattga aaacctgaaa atattagaag
    12181 acatccattt cactatccct aaagatacct ttacatcgat tgtcgcgcca agcggagccg
    12241 gtaagtcaac attgctgaaa actttaacag gagtccagcc tatgacttct ggaacgatca
    12301 aagtagacga tcaaaaagta actggtctcc atacagcatt tagttatatg ccgcaagaag
    12361 atatgttact tccttggctg agcgtctatc aaaacgttac gttatatcaa aaaatcaatc
    12421 atctaacaat caatgaagaa caagtaaggg attatctgga tatctttggg ttaaacggct
    12481 atgaacattt tttgccggaa caattatctg gcgggatgaa gcagcgaacg gccttgttac
    12541 gaacaatcat gaatccttct tcttacttgc ttcttgatga accatttgga gcgttagatg
    12601 ctatgaccag agggcagatg caagactgga tgttgaagct gcctaaaaaa gcaaagcgga
    12661 ctactttact tgtgacgcat gatatcgaag aagcaattta tttatcagac aggattcttg
    12721 tgctttccgc ccgtccggca catgtgattg ctgagattca agtgcctgaa aaacgacggt
    12781 cacgtgaatg gctactgcag caatcagagt tgaagcaaac gatttatcag ttattggcag
    12841 gtgaattaga tgttaagtga tgctcattgt catttagatg ggagtcagag tttagcactc
    12901 cttcagcagg aacacacgat tttgacgatc atcaattgtg attcgccaga agaatggaag
    12961 gaaaacagac agcttgcagc aagtaagact caagccttaa gttacggtat ccatccttgg
    13021 aaagcagata gctatacgtt tgaacaagtt gagccttttt tgaaaaaagc aaggatcatc
    13081 ggtgaaatag ggttagataa tatttggacg aatgtaccga tgactacaca aaaaaaagta
    13141 tttgagcgac agcttgcttt tgcagccata aatgagaaac ctgttgtact gcacacaaaa
    13201 ggatgcgaaa aagagatctt aaattatatc caagcttacc ctaatcacta tttgatccat
    13261 tggtattctt ctttggacta tcaacaagac tatattgatt taggctgtta tttcagcatt
    13321 ggtcttgatt tgaaaaagaa tccagcagtc tggcaactag ctaaagcagt cccaatcgac
    13381 cacttactta ttgaaacgga tggactacat gcagcagagt gggctttagg ggggattttt
    13441 tcagaaaaag attatgtctc actgcttgaa gaacagttaa agacaatcgc tgagatcaaa
    13501 gagctatcag ttgaagaagt taaaaaaaat acttatcaaa acttggaacg tttcattaga
    13561 ttaggtaaat aaaaaagaga tgtgacacaa ctagtgtcgc acctcttttt ggctttcttt
    13621 gtataaaagt aaactgtttg attaataaat gaatcttagt accaaccgtt tgcttcccag
    13681 aatgctttcg ccgcttccca agaaccataa cgtgaagtaa catattgttc tgctactctt
    13741 tcttggtttg cagcagagta gtcaccattt aagtaagaag aatctaattg gtaacgtccg
    13801 atgtaacgtc cgttcgttgc tgtgtaagaa ccgcttgatt ctttttgtgc gatccattct
    13861 tttgcagagc ttgtgcttgc tggagtagca gcagtttctg ttacagcagg agcaggttgt
    13921 gtttcagcaa caggcgctgc ttgtttttcc gtttgaaccg tttcttgtac aacaggttgt
    13981 tcagcttgga ctggtgcttc ttgaacggct gcttgaggtt cagcagctgc ttgtgcagag
    14041 ccttctgttg ggattgttaa ttgttgtcct gagta
//
LOCUS       DACDRN010000058        14058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDRN010000058 DACDRN010000000
VERSION     DACDRN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.058"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..889
                     /locus_tag="IWJ80_002076"
     CDS             <1..889
                     /locus_tag="IWJ80_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP9461782.1"
                     /translation="CQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIED
                     KMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRIL
                     PNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANIN
                     HYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFS
                     LLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGFK
                     SFENMRIRIFLINQLIKVR"
     gene            complement(1052..3172)
                     /locus_tag="IWJ80_002077"
     CDS             complement(1052..3172)
                     /locus_tag="IWJ80_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9461783.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            3402..4580
                     /locus_tag="IWJ80_002078"
     CDS             3402..4580
                     /locus_tag="IWJ80_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP9461784.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            4596..5354
                     /gene="aroD"
                     /locus_tag="IWJ80_002079"
     CDS             4596..5354
                     /gene="aroD"
                     /locus_tag="IWJ80_002079"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP9461785.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            5377..5862
                     /gene="ybaK"
                     /locus_tag="IWJ80_002080"
     CDS             5377..5862
                     /gene="ybaK"
                     /locus_tag="IWJ80_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP9461786.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(5933..7186)
                     /locus_tag="IWJ80_002081"
     CDS             complement(5933..7186)
                     /locus_tag="IWJ80_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9461787.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            7302..8651
                     /gene="gdhA"
                     /locus_tag="IWJ80_002082"
     CDS             7302..8651
                     /gene="gdhA"
                     /locus_tag="IWJ80_002082"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP9461788.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            8763..10109
                     /locus_tag="IWJ80_002083"
     CDS             8763..10109
                     /locus_tag="IWJ80_002083"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP9461789.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(10417..10803)
                     /locus_tag="IWJ80_002084"
     CDS             complement(10417..10803)
                     /locus_tag="IWJ80_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9461790.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            11682..11894
                     /locus_tag="IWJ80_002085"
     CDS             11682..11894
                     /locus_tag="IWJ80_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461791.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(12172..13971)
                     /locus_tag="IWJ80_002086"
     CDS             complement(12172..13971)
                     /locus_tag="IWJ80_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9461792.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4621 a         2546 c         2759 g         4132 t
ORIGIN      
        1 ttgtcaggta tctttgacca ccgttattcg tacgttgaaa gagtttaaaa gctacttacc
       61 aaagcaatct aagaggattc tccccagagt attgatggtt gatgaatttc gttcgcacgc
      121 ttccatagaa gataaaatga gctttatttg cgcagatggc gaaacaggac aattaattga
      181 tgtcttgcct acgcgtaaat tgcctcgatt aacaagctat ttcttaggct gcgctaatcc
      241 tgaagaagta aaatttttag tcacagacat gaacgccgcc tacttccagc ttaccaaacg
      301 tattctgcca aatgcgaaag tagtgattga tcggtttcat attatcaaac acatgaatca
      361 agcctttaat gagttgcgta tccgtgaaat gaatgaactt cgtaaagtag gacagaaaag
      421 tcaggcagaa aaactgaaaa agaactggcg ctttttgcta aaaaatcgtg caaacatcaa
      481 ccattatgaa tacaaaacat ggaaaagttt tcgagcacca aaatacccat ttcttacgga
      541 agcgatgatg attgatcgat tgcttggctt ttctacgtcc ctgaaggagg cgtatcccta
      601 ttttcatgaa ttagttgaag cctttcgaga caaagaccct gacttatttt tctccttatt
      661 ggcagaactt cccgaaacgt tagatgacgg ctttcgggaa aagctccaaa accttctgac
      721 ctatgaagaa ggcatcacca acgcgatgat ctatccttac accaatggaa aaatagaagc
      781 gaagaatacc catataaaga caatgaaacg agtatcctac ggatttaaat catttgagaa
      841 catgagaatt agaatctttt tgatcaatca attaatcaaa gtaagataac aaaaaatctg
      901 agccagaatg aggttcattc tgacccagat ttgattttgt acaacccatc agtccttatt
      961 gacaaagagc caattattct aagatttctc tttaccaaca tcatttgaca aagagcctaa
     1021 ttttgtcgct gctcctttta aatttatttt atcattcgga agaacttgtg gtcgttccgt
     1081 gaagttcttg gtacgtttgc gttttataaa gatcaacagt tgattgtccc ttgtgttcac
     1141 taaagacact agttgacttg tcaccaagag atttttcagc ttcttttaat ttctctaagc
     1201 cattcttgta gtcataatca ctggtatcaa ttgggtctaa tccactattc gtatagaacc
     1261 gaagaagatc accattattg atttggtcag aaacagctaa ctgtttatca actttttctt
     1321 tcagtgaatc aatttcattc tgtacttcct cagtaggttc agtaatagct tcaccagttt
     1381 cgttactata gatcgtacca ccgtagtaag tatattttgg tgtaacaaaa tcaccatcac
     1441 ggaaagaaac aagctgttca tgatctttcg agaatagatc ttgtcctaac tgaatataat
     1501 tttttgtatc tacacctagt aagtgaagta atgtaggcaa ggcatcaact tgtccgccgt
     1561 aagtgtgatt gattccgccc tgtgtttgtc cagggatatg gatcatataa gggacacgct
     1621 gcatttgtgc attgtcgtag tttgtccaag tagaacttgt ttttccaact aattcagcta
     1681 gatttttatt tcttgaattc gagacaccgt aatggtcacc gtaaaggacg atgactgaat
     1741 tatcatacaa gccgctttct ttcaaataat tgaagaattc ttgtacggcg gtatctaaat
     1801 aattggctgt cgcaaagtaa ccgttgatcg tttcatcatt tgtattggct aacgggaatc
     1861 ctgcttcgtc attggtgaat tttgaataag gataatggtt agatacagca atgaattttg
     1921 aataaaatgg ttgttgcaga cgttccaagt attgtacgga ttgttggaaa aatggtttat
     1981 catgcaaccc gtattggaac gaattgtcat cattaacatc ataataagaa gaatcaaaga
     2041 aataatcata tcctaaattc ttgtatgtct cgtttcgatt ccagaatgta ccagcgttcc
     2101 catggaatac cgcactagtg taaccttgtg tttgacttaa aatatcaggt gcagcttgga
     2161 aagtattttt tccgcctaat tgtgtgaaca aggagccttg attcaagcca aacaatgaat
     2221 tttccatcag cgtttccgca tcgcttgttt ttcctgcttt tacttgatgg aaaaagttgt
     2281 caaaactgaa cgtgctgttg ctgtggtaaa gactattgat gaaaggtgta acttcaagct
     2341 ggagaccatt ctcatctgtc aatttataat cgatcaggaa ttgctggaag ctttctaaat
     2401 ggatataaat cacatttttt cctttagcga gcccgaacaa atcatcattt ggtgctgcat
     2461 aatgttcttt tacataattt tctacttctt tcatatcatt gggacttgct tctgcccgca
     2521 cttggtttgt ctggtaagtt tgaacaccgt cataaaccat gaaagcattc aagccaagat
     2581 atttgaccaa ataatcacga gagaatgttc gagtcagcaa ttctggacga tcagcttccg
     2641 ccataaaaag atttcctgag aaaatcataa ctgctaatgt agagatcgca aaagccattc
     2701 ttgctcggat cgggcgaggg tccaacttga ttttttttgc aatcagcaga acagtcaaga
     2761 taataaaatc gatccaatag atcacatcat atgggcgaaa cagtcgaatg gcactttctc
     2821 ccagaccact cgctactttc ccagcaccta aaatcgtgtt gatcgtaatg aagtccgtga
     2881 attctcgata ataagtgaca ttcgaaaaaa caagcaatgt caaaagaaaa taaatgacca
     2941 tcatcgtgat ataagcaact ctagggcgtc tgatataaag agcaattcca agtaataaca
     3001 atgtagttgc aatagggttg ataaacaaga taaagtattg aatgccactt tcgattccga
     3061 gatggaaatc aactaaatag gcaaaaatcg ttttcaacca aagtaaaaca actagtaaag
     3121 aaaaaaatcc gagcctctta ttcaaaaagg caggtgtttt tatacatttc aagataatct
     3181 tcctttctgc acttcttaat cacagaagtg tcatgaaacc atgttacaca gctcacttat
     3241 tctcatttat tttactagta tgacaatata gtgtcaattg aaaccagaag tttatgctgt
     3301 ttcattaagg ttttctttaa gcttcatgaa aaaatgagca gtaagacaag tcctatgttt
     3361 tttgttatac ttgggaagag aaagctaaag caggtgaagg aatgaaagta aaagtaaaaa
     3421 aaagagcaag caatcgattg aaaaaaggat atccactgat ccaaaaagaa gaccttgctc
     3481 atccccaaaa agaaaccacg caatgggtgg aattagttga ttcgcaaaac caatttatcg
     3541 caaaaggata tctaggaaag cagaataagg gaatcggttg ggtattgagc cagcagaaca
     3601 tcccatttga tcaatctttt tttgaagaat tttttgtgaa gtcaaaagaa aaaagaactt
     3661 tctttttttc agatgattct actaccgctt ttcgtatatt caatggagaa ggagacggga
     3721 tcggcggtct gattgttgat tattatgcag gttatgctgt tttttcttgg tataacgaaa
     3781 cgttatatgc acatcgtgaa ttattgattc aagcttttcg tgaaatctac ccggaaatcg
     3841 taggaggcta tgagaaaatt cgatttgaat cagtaaatct gcctgagagc cagttcattt
     3901 acggaaaaga ggcaccggaa cccttgatcg taaaagaaaa cggagtaaaa tttgccgttt
     3961 atttaaatga aggattaatg actgggatct ttttagacca aaaagaagta cggggatcat
     4021 tagcagaagg tttcgcttct gggaaaacag ttttgaacat gtttagttat accggcgcct
     4081 tttctgttgc tgccgcaatg ggaggggcaa tcgaaacaac aagtgtcgat ctagcaaaac
     4141 gcagtttaaa aaaaacacag gaacagtttg aagtgaatgg tttggatgtc gatcagcaaa
     4201 aaatcattgt tatggacgta ttcgagtatt tcaaatacgc caaaagaaaa caatttttat
     4261 atgacatgat tgtattggat ccaccaagtt ttgccagaaa taagaaaaaa gtctttcgcg
     4321 tagcaaaaga ttatggggaa ctggtcaagg atagtctgga tattttagca gaagacggga
     4381 tacttattgc gtctacaaat gctgcgaatg tctcgataga acaattccaa acaatgattg
     4441 aagaggaatt ccatgccaaa aatgtttctt ttgaacaact tgctttatat cgtctgccaa
     4501 aagatttcca gacgacggat acctttttag aaggcaatta tctgaaagtg tttgtttatc
     4561 aaattaaaaa taaccattag aaggagcgat gcagaatggt ttgggtaaag ggattgaata
     4621 taggaagcgg tagaccaaag atttgtgcgc ctcttatcgg ggccgtccga gaggaagtat
     4681 tacgtgaagc agttcttgca cgtgaagcag gggccgattt agtcgaatgg cgagtcgatc
     4741 attatcgtga agttttttct tttgatgaag tagcagaaac attggttctt atccatgaga
     4801 tgcttgatgg actgccgtta ttatttacgt ttcgtacctt agctgaaggc ggagaacaag
     4861 acatatcgat ccgtcagtat cgcgaccttt atgaacatgt gatacacaca ggattagtag
     4921 atatggtcga tatcgagttg ttcaaagtag aaagtctagg aaaaagactg atagaggaaa
     4981 tcaagtcttt aaacattcct ttaattatca gtagtcatga ttttaaagaa acaccagcag
     5041 accctgtctt gctttaccga ttgaatgtta tggaacattt tggagccgat atcggaaaac
     5101 tggcagtaac tcccaataat gaacgagatg ttttgcggtt gatggagttg actagacggg
     5161 caaatgcttt tgtctccatg cctattatta cgatgtccat gggaaatctt ggtaaaattt
     5221 ctcgtctggc tggcggaacg acgggttccg ttatgacatt tggttcctta agcagcgatt
     5281 ctacttctgc tccaggacaa ttaccggtca aagcattgaa gcaaattatt caattattaa
     5341 atgaaccgtt gtagaacaag tttgaggtga tattttttgg caaagaaaaa aatagtaaaa
     5401 acgaatgcaa tccgtatggt cgaacaaaaa aagattccct atacagagca cgaatatgag
     5461 tgggacgaaa gccacctaag tgcgtcatcg gtcgctgaac agcttccaga aagccaatct
     5521 catatattta aaacactggt tgctgtgggc aatgtgacgg ggcctcttgt tgctgtgata
     5581 ccgggagaag ccgaattgaa tctaaaaaaa ttagcaaaag tttcagggaa taaaaaagta
     5641 gaaatgctcc atttaaaaga tttggaagca acgaccggat acatacgtgg tggctgctcg
     5701 cctataggta tgaaaaaact atttcctact tatttagatc aaattgcaga aagctatgaa
     5761 caaatcattg tctcggctgg cagaagaggg ctacagatgg agttagcacc tcaagatatc
     5821 tgtgcattaa cgagcggaca atttgcagat ataaaacaat aaatcatacg tataaaagaa
     5881 agaaggaggc cgggatgttg tttgtccagt ctccttcttt cttttttata cattatgctc
     5941 tttgcccggc attcattcct gctacatgcc ctgtagcgaa agccgctgtg atattaaagc
     6001 cgccagtata accattgaca tccagtaatt ctcccgcaaa atacagacca tcaatgattt
     6061 tactctccaa tgttttagga taaatttcct ttagagaaac ccctccgcca gttacaaatg
     6121 atttctcaat aggaaaagtt ttgttgattg atagcttaaa ctctttgcat agttgaacaa
     6181 aatcttgaat ttgtttttga gaggcttgtt gtcctgtcag atgatccatt tcaagccgct
     6241 caagaaagaa aactaacaat cgctcaggca atataccatg ccaagcattg accaagttct
     6301 ttttggttgc tttacttttt tctgtcaata tatgaataag ctcttgtttg gtttgtgtag
     6361 ggaaacaatc cagagaaagt gtcactggtt ctcccgtttt ctctaattct ttattgacaa
     6421 aacttgaaca acgcagtgca gccggaccag aaataccgaa atgggtgaaa agtaaatcca
     6481 tcgtatgttc agtcaataca cgaccttttt tatttaaaac gcgtaaagtg atgtcttgta
     6541 aagaaagccc ttgaagtgtt ttctcctgaa tatacggttc atccgatatc aagggggact
     6601 ctgtcgcata aagaggcgtc accgtgtgac ccactcgctt cacgatcttg tacccatctc
     6661 cagtagaacc agtagatgga taagtccgtc caccagttgt taagataatg gcaggagcat
     6721 ggaactcttc gaattccgtg cgtataccgt aaattttatg ttctttgtgg atcaatttct
     6781 ctactcgagt agagaataga agcgtcacat ctagttcttt cagacgattg agcaatgctt
     6841 cgataatcgt ttttgattta tttgttacag gaaacatccg tccatgatcc tcttctttta
     6901 aatgtacgcc ttgtgattca aagaagttca tgatatcgaa attattccat tgcgcaaacg
     6961 tactatataa aaactttcca tttccaggaa tatgtgcaat caaatcatca acaggacgat
     7021 tgttcgtaac attgcaccga ccaccacctg tcatcagtag tttttttcct gcttttttgt
     7081 ttttttcgat caagagtacg cgtgcgcctt gttcagctgc actgatcgct gccatcatcc
     7141 cgcttgttcc agcgccgact acgatcacat catacagttt ttccattccc tcacctcaag
     7201 gaaagtgtag catatctgaa gaaagaaaac agaaaaaatg ttttgagcca aaagagaaaa
     7261 tgagctaaaa tgagggaaga taagcaaagg agcgtggaac gatgacaaac gcaacagaat
     7321 atgttcaagc aattcaagaa aagctacatc aaaaggatca agggcaagaa gaatttttac
     7381 aagcaatcga tgaattcatg cccacggtta tgtctttttt ggacactcat cccgaatata
     7441 tcgaaaagaa tatcttagaa cttttaacgg aaccagaaag agttattcaa tttcgagtgc
     7501 cttggcaaga tgatgaagga aactggcgag taaatagagg gtaccgtatc caatacaact
     7561 cagctatcgg cccttataaa ggtggattgc gcttccatcc tagtgtcaat ctaagtatct
     7621 tgaaattttt ggcttttgaa cagattttca agaatagttt aaccggtctg ccaatcggtg
     7681 gaggaaaagg cggaagtgac ttcgatccta aaggaaaatc tgataatgag atcatgcgtt
     7741 tttgtcaaag tttcatgctg gaattgtcaa aacatatcgg cccatcgatt gatgttcctg
     7801 cgggagatat cggtgtagga gctagagaaa tcggctattt gttcggcgaa tataaacgat
     7861 tgaaaaaata tgatactggt gtgttgacag gtaaaccttt ggatttttgg ggaagtaaga
     7921 tacgtacgga agctactggt tatggattag tgtattatgt caaacatcta ttgaacgaag
     7981 aaaaagattc atttaatcaa aaaacagttt ttgtttccgg aagcgggaat gtagccattt
     8041 atgcaatcga aaaagtgcaa gagctaggcg ggaaagttgt tacatgttca gattctagcg
     8101 ggtacatcta tgatccagaa ggaatagatg tgaaactatt gaaagaagtc aaagaaatca
     8161 atagacaacg tttgacggaa tacgcaaacc aacgtccttc tgctatttat catgctggag
     8221 aatctgtttg gacattgaaa gaaaaagcgg atatcgcttt gccatgtgct actcaaaatg
     8281 aaatagacga aagcttggcc gaaatcttag tagaaaatgg gatcaaaatc gtagcagaag
     8341 gcgctaatat gccatgtaca gtaaaagctg tttctgttct tcatgaagca ggcgtgtggt
     8401 attgtccagg aaaagctgct aatgctggag gagtagcagt atctgcttta gaaatgagtc
     8461 agaacgccca gcgattgact tgggaaagag agcaagtgga tgagcagctg gatcaaatca
     8521 tggaaacgat ttaccatacg tgtcgccaaa cagcagccga gtacggcgac gaaaaaaatc
     8581 tgcttcttgg agcaaatgtt gctggatttg aaaaagttgc caaagcaatg gcagcagaag
     8641 gtttagtttg aaaaatgaaa ctttcttgga aatattgtca gcattttgtt tatttgcttg
     8701 aaaaataata aaaaaaaacg taaagtatta cctgtagagt aaacctataa ggaggagaac
     8761 aaatggcaca cattcatttt gactattcaa aagtagcacc gtttgtcaac gaacaagagt
     8821 tagggtacat gcaaagccaa gtaacagcag cccacaacga attacgggaa ggtactggcg
     8881 caggaagcga cttccgcggc tggatcgatt taccaacgaa ctatgacaaa gaagaatttg
     8941 cacgtatcaa agaagcagct aaaaaaatcc aatctgattc tgaagtatta gttgttatcg
     9001 gtatcggtgg ttcttattta ggcgctcgtg cagcgatcga cttcttgaat cacacattct
     9061 tcaatttatt agatagcaac acaagaaaag caccacaaat cttctttgcc ggaaattcaa
     9121 tcagctcaac atatattgct gacttgatcg aagtaatcgg tgatcgcgac ttttcagtaa
     9181 acgttatttc aaaatctgga acaactactg aaccagcgat tgcgttccgt gtattcaaag
     9241 agcttcttgt taagaaatat ggacaagaag aagcaaacaa acgtatctac gctacaacag
     9301 ataaagcaaa aggtgcagtt aaagtagaag cagatgcaga aggttgggaa actttcgtga
     9361 ttcctgatga catcggcgga cgtttctctg tattaactgc tgttggtttg ttaccaatcg
     9421 ctgttagtgg tgcagatatc gatgcattga tgcaaggtgc cgctgatgca agcaaagcat
     9481 actcaagcga caaactagaa gaaaacgaag cttaccaata cgcagcaatg cgcaacattc
     9541 tttaccgtaa aggcaaagta actgagttgt tgatcaacta tgaaccagga atgcaatact
     9601 tctcagaatg gtggaaacaa ttgtatggtg aatctgaagg aaaagaccaa aaaggcatct
     9661 atccttcaag cgcaaacttc tcaactgatt tgcactcttt aggacaattc atccaagaag
     9721 gacgtcgcaa tatttttgaa acagttgtga aggttgaaaa acctcgtaaa tcaatcacaa
     9781 ttcctgaaca agcagaagat ttagacggtc taggctactt gcaaggaaaa gaagtggact
     9841 tcgtaaatac aaaagcattt gaaggaacat tgcttgctca tacagacggt gacgtgccta
     9901 acttgctagt caaaattcca gaaatggatg cttatacatt gggttataca atgtacttct
     9961 ttgaaattgc tgtaggtatt tcaggttact tgaatggaat caacccattc gaccaaccag
    10021 gtgtagaagc ttacaagaaa aacatgttcg cacttctagg caaaccgggc tttgaagatc
    10081 ttgctaaaga attgaacgaa cgcctgtaag ataagggttt ttcgctagat ttaaataatc
    10141 agtgatagaa acatgttcgc tttaaaatga aaactggtac ccatttaggt actcaaacat
    10201 actgcatcgt ttattcagaa taaggggctg tgacaagacg tttgtcacag tcccttgttt
    10261 gtatagaaat cgattgattg ccttgctctt tagtttgttt taggacttta agaacagtga
    10321 ggctgacttc gaaggttaat tctgtttttt tgttttctag aatcagtaag caaaaaagca
    10381 ctcacttcga gtgctttttt gcgatatatt cttagcttaa gataggccta atttttcttt
    10441 tacagatgag ggtaccttat cgctggtaat tacttttttc tctatgatcc gcttttcgct
    10501 tgctttcaac tgcaagatcg ttccttcggt gtaagcagaa ccattggcag agtcaccaga
    10561 agtaatgaca tcgaaatcta actgtctttt ttcaccgttt tcagcataag catctacgtt
    10621 gtatttaaaa cctcttccaa ttacttcccc attatctgct ttgatatcag tttctgcagg
    10681 cattggagct ttgactaccg cataataatc tttacctaca taagtagact gatagtagtc
    10741 ccaacctttc catcctataa aagcaatcaa tatgataacg atcaacccta aaattttttt
    10801 catgaaagat ctcctttttt atttttatta tattaaatat tatacagata ataacggaat
    10861 attatcatcg aaacagctaa caaaattgtc ataaaaaacg agatggttga tattagaact
    10921 tgtgtttttg tttaggtcta gagataaaga gagacaattt ccaaatatat gtaatgaata
    10981 caaaaagacg tttgtcgcgg attaccgtta caaacgtctt ttattgattt tccgagatct
    11041 catcagtcgc catttggaat ggttcactcc ttatctagat tcgtttaatg ctcggactgc
    11101 ttgttcgatt gtaataccaa acgcaacgtt attttcgttt tgtgcatcaa tcgccataaa
    11161 acggttagaa gttgtatcaa aagctacgcg gtattgcata tttgtttttg taaacgtcat
    11221 aaaattcatc tcctttgtaa tgtaatttag ttcttgaaag tttcctttaa actttcgaac
    11281 ttataccaag tttagcatcg tacgtttttt gatgcaatta aaaagcattt atcattttcc
    11341 ttatttattt tttcttaaat ggtttagcta ttttctttta atccgataag tagcccaatc
    11401 atcaaggtta caaatactat tgcttgaata taaatcatat gctcacctct ttataaataa
    11461 cttagcaata taaagaaaaa aacgcacgaa caacatagga atataaacaa acgtttttgt
    11521 cacaacgtct aagatcgtgt taaaaaatag ttatctaaaa agcagaaaga tactcaaaat
    11581 aaaaaataaa aaaatgaata aaaacgtttt caaatacaag gtaatttgtt attataaaat
    11641 aaagaaaaaa caaaataaaa ataaagaaaa gagggaatga aatggtaaaa gcagaagtag
    11701 gaagatctta tatatgcaaa ccgatcggag tatctcatga actaatcggc tgtattgaac
    11761 gtacatatat caacacggct gtcgtgtatg ttgaaagtta tgaccaacga gactatcaac
    11821 ttatcaatga aagcaagtat cgcatgctcg taaaattctg tgatattagc gcacctactg
    11881 aattggtttc ttaaacagta tttttgagaa ttcgcaagaa ctcccttata atataaataa
    11941 agacagaagg gagcgagaag atatgccaaa aagaaaaagt tcagggaaaa tgggaattat
    12001 tcttgcagtg atctctgcaa ttcctgctat tttagctatt gttcgtgcga tgaaggaatc
    12061 aagagaaagt taagcacaca ttatgagcgt caaacaaaaa acgcatatac ctgcggtggc
    12121 aggttatgcg ttttttgttg tataaagact tttttcatta acgatttttg attagccgac
    12181 tcccaaaaca aaatttagca gtttatctga ataagtgatt tcttcatctg tttgtttcac
    12241 actttcattt agtagtccca tctcatcggt tatgatacca tccacaccat aaaacatcat
    12301 gcgactcatg gcatctgaat cgttgattgt ccatacatat acttcttttc cgtcttggtg
    12361 ggcagaatta atgaagttac ggttgatcgt actatattcc attgtcagaa aatcagcagg
    12421 cgtcacaggg ggcccaacaa tattgaaagg taagatgtag cccacataaa agtttggttc
    12481 tgcttctttt aaatctgaaa cggtttggta cgtcaaactt tgcaaaatat gatggttctc
    12541 taagataact ttgcggtatt tatggacaaa tcgttcgaca aggtcatccg aatctttttt
    12601 agaggtcttg atctcaataa gtaatttttg gtttagctta tgtgcttttt ccaaataaga
    12661 atcaaaagaa caaacagggg cttgcactcc gttttcagta actgtaagat ttgtcaattc
    12721 tttcaatgta agatcctgtg gtgttccatt tacccctgtt aagtgacgaa gatttgcatc
    12781 atgcatcact acgaaatgac catcttttgt ttcctgaaca tccatctcta cataatcagg
    12841 atgatagtct ctgcttgttt tttcaagtgc ttctaaagta ttttgcgccc cattttgggt
    12901 actcacacct ctgtgagaga cagtcactgg ttttgtctga acagctgaag tcagataatc
    12961 catattgtaa agacagacgc caataccgaa aaaaactgca aaaagagtca aaccagtgtt
    13021 tttcagcgca gagaaacgca agttgggggc ttgtgggata aaccacttag ggatctcagg
    13081 caagaagcct tcgtcatcca tgaaatcaac aatgatataa aaaatcccta cggtagacat
    13141 cacgatattg aataataaaa tcccttgtaa aagcgtcata gcaaacacag cgctgatcaa
    13201 tgcatagtct gggaaaaatt gttcaaccat actttgtgcc aaaataacaa cgatatatcc
    13261 taaagaagaa aggagtaaaa tcgtaccgcc aatcacaata aattggcccg tgatggccaa
    13321 aagacgcgat ttggtcaatg accagctttc ccgaatggct gctcgaaaag aacgatctcg
    13381 tagaatcatt tccggtaaag caaaaatcag tcgaatccca atatagccaa ggaaaatata
    13441 aacgagtaaa aaggaactga cgatgatcca acgattagca aaaataaaat ccataatgaa
    13501 agcggggatt tttatttttg acagtagatc agaattaaaa cttaatccgc tgatcggtag
    13561 aatcaataaa aaataagcaa ggaaaaacaa aaaagtaatg ggtctcactt tcttcagctg
    13621 aagtatcgtc aagtgtaaga gttgcttcaa agagattggt tctttctttt taataaaaaa
    13681 aacactcatc aaaagaaaag taaattcgaa ataaactaac agtaaaatcg tcatcaaaac
    13741 tacgattaac gagagtagaa ccccaggatg ttgagaaaaa attgaaggaa tattgtcata
    13801 agacaaatag tcaattgccc ctcggtgtaa aataaaacgg gtcgtactgc tcaataaggg
    13861 taaacaaata aataaaataa agccatgcat cagcaagaca tcacggaaat aagcttgtgt
    13921 tccttttaaa aagtcccaag cattacctaa gctacgtttc aaataattca tcctatcacc
    13981 tgctcttcac attgtagcgc aaatctaatg aaaaaggaaa gggtgcagtg tgttttgcag
    14041 accctttccc agatttaa
//
LOCUS       DACDRN010000059        13969 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDRN010000059 DACDRN010000000
VERSION     DACDRN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.059"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(325..2322)
                     /gene="tkt"
                     /locus_tag="IWJ80_002087"
     CDS             complement(325..2322)
                     /gene="tkt"
                     /locus_tag="IWJ80_002087"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP9461793.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPIGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2424..2675)
                     /locus_tag="IWJ80_002088"
     CDS             complement(2424..2675)
                     /locus_tag="IWJ80_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP9461794.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2822..3445
                     /gene="lexA"
                     /locus_tag="IWJ80_002089"
     CDS             2822..3445
                     /gene="lexA"
                     /locus_tag="IWJ80_002089"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP9461795.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3624..3842
                     /locus_tag="IWJ80_002090"
     CDS             3624..3842
                     /locus_tag="IWJ80_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461796.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4130..4642)
                     /locus_tag="IWJ80_002091"
     CDS             complement(4130..4642)
                     /locus_tag="IWJ80_002091"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP9461797.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="IWJ80_002092"
     CDS             complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="IWJ80_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP9461798.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLMHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPVE
                     FLDLVAIGTIADLVSLTDENRILVSLGINAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSN
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     IMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7095..7529)
                     /locus_tag="IWJ80_002093"
     CDS             complement(7095..7529)
                     /locus_tag="IWJ80_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP9461799.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7539..8327)
                     /locus_tag="IWJ80_002094"
     CDS             complement(7539..8327)
                     /locus_tag="IWJ80_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9461800.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETDFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8324..9265)
                     /locus_tag="IWJ80_002095"
     CDS             complement(8324..9265)
                     /locus_tag="IWJ80_002095"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP9461801.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9442..10029)
                     /locus_tag="IWJ80_002096"
     CDS             complement(9442..10029)
                     /locus_tag="IWJ80_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP9461802.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10250..11563)
                     /gene="obgE"
                     /locus_tag="IWJ80_002097"
     CDS             complement(10250..11563)
                     /gene="obgE"
                     /locus_tag="IWJ80_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP9461803.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11851..12861
                     /gene="gap"
                     /locus_tag="IWJ80_002098"
     CDS             11851..12861
                     /gene="gap"
                     /locus_tag="IWJ80_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9461804.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12946..13881)
                     /locus_tag="IWJ80_002099"
     CDS             complement(12946..13881)
                     /locus_tag="IWJ80_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9461805.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3856 a         3130 c         2455 g         4528 t
ORIGIN      
        1 gagcaatcgc ttttttattg atcaccattt ttcttttagg tctatggtgt cgggcactgc
       61 gggtcttcag ctcttgcatt caaattcctc ctcaaaataa aaaaacttaa taaaaagttc
      121 tcttcttaaa acaaacttaa ttaattaaat tatactttta attctcaaga aacacaaact
      181 aatccgaaga attctttgtt ttgtcattta aatgtaacat agccgaaaac gaaaagtaat
      241 attgatttat tcttctaaca cataaagaac agctgtctcc aacttgcaga cagctgttct
      301 tatcagtcaa acaattgctt ggatctatga tagttgattg tacgtattca cgacattttc
      361 tactgtaaat ccaaattctt ctaaaatttt agtgccaggg gcagatgcac cgaaatgatc
      421 gattgtaatc gttgcgccct ctgttcctac gtagcgttcc caaccaaatg atgcagcagc
      481 ttcgatagcc acacgtttct tcactgattt aggaagtaca ctttctttat attcagaaga
      541 ttgtttttca aagaggtcaa agcttggcat agaaacaaca gaaacatctt tgcctttttc
      601 agccaattct ttttgagctt tcacagccaa atcaacttca gaacctgttg caatcaaaat
      661 accttctggt gtttctcctt gtgatggaga aagaacatac gcgccttttt tcaccatttc
      721 atctgctact tcttttgtag aaggcaatac tggaaggttt tgacgactca atacaaggat
      781 agtaggcgca tctgttgtct ccatcgctac tttccaagca gcacgtgttt catttccgtc
      841 tgccggacga atcacttgaa caccaggcat gcagcgaaca gatgctagtt gttcaatcgg
      901 ttcgtgcgtt ggtccgtctt ctccgacagc aaccgagtca tgtgttaaga cgtaaacaac
      961 aggagtatgt tggattgctg ctaaacgaac tgctggtctt agataatcga caaagacaaa
     1021 gaatgttcca ccgtagatac gagttccgcc atgaagttgg ataccattca tagccgaagc
     1081 cattgcaaat tcgcggacac cgaaccagat gttgcgtcct tcataatgtt caggagtgaa
     1141 atctttatct gcagccacca ttgtattatt ggaacctgac aagtccgctg aaccacccca
     1201 gaagcttggt atagctttcg ataattcttg gatgacttct ttacttgaaa cacggcttgc
     1261 ttgactgctt ccaacttcat aagttggcaa ttctgcatcc cagttttctg gtagtttgcc
     1321 atcaaaagca tcttcaaatt gttgtgctaa ttcaggataa gcttttttat aattggcaaa
     1381 catttcattc catgcatctt cagctttttg tccttcttct atcatcgttt gatggaagcg
     1441 ggcagctact tcttctggaa cagtgaaatc tggatactcc caaccataaa cttctttagc
     1501 catcttgatg ccatcttcac caataggagc accatgaaca gcagatgttc cttctttagg
     1561 agcaccatag ccgatgactg tttttacttc gatcaatgtt ggtttgtctg tttcagcttt
     1621 tgcttcttca atcgctttag agattgcttc taggtcattt ccgtctttca ctaagatatg
     1681 ttgccagcca tatgcttcat agcgagcacc aacgttttct gtgaaagctt ttgaagttgg
     1741 accgtctagt gaaatatcgt ttgaatcgta taaaacgatc aatttcccta gtttcatatg
     1801 acctgccatc gaacttgctt cttgagatac cccttccatc aagtctccgt caccgcatat
     1861 tgcatacgta tagtgatcca tcacattaaa gttttctttg ttatacgttg ctgcaaggtg
     1921 cgcttctgcc attgccatac cgacagccat tgcaatccct tgtcctaatg gccctgtagt
     1981 ggcttctaca ccatctgtgt gatgtacttc aggatgtcct ggtgttttag aatcccattg
     2041 acggaattgt ttcaaatcat cgattgtcac ttgatagcca gctaaatgaa gcaagctata
     2101 caacattgct gacccatgtc ctgccgaaag aacgaaacgg tcgcggtcag cccagttttt
     2161 tgatgtcgtt ggatttactt tcaggtgttt tgtccataac gcatatgcca taggtgctgc
     2221 gcccatcggc aatccaggat gtcctgagtt tgctttttgg actgcttcaa tacttaatgt
     2281 acgaattgtg ttaacgccaa gttggtcaat tttgtcaaac aaaataaccc ctcctattaa
     2341 tttaggtttt ttttaccttt atttcttaca ctttcattct ataagatgtc acacgaaaaa
     2401 gcaatctttt tcagtatttt ttactaaaag ttgtcatttc ggttgtgtaa gccttttttc
     2461 ttttggattt cttttaattt ttccggggtc acgtcattgc cctcaggatc gacgactttc
     2521 attccttcga tatgatgacg cataccggaa cgaaaagctt ccaaatactc tttgcgtaag
     2581 ttgatttgtt cttgttcttc ggcttttgag agaccatttt ccttcttttt acgtgcaagt
     2641 tcattgatcc tctctatttt ttcaggtgac agcatgctca gccctccttt tactctcctt
     2701 catattatat aaaaactgaa aaaaaaacaa ctagataaac taaataaaat acgaacattc
     2761 gtttgattta gcgcttttta tatggtacta tttatttatc aagtgaaaga aggtgtacct
     2821 tgtggcccga caaactgaat caagacaaat cgaagtctta cgatatatcc atgaacaagt
     2881 aacccaaaaa ggatacccgc cgactgtccg tgaaatcggg gaagctgttc agctttcttc
     2941 tacttctact gttcacggtc atcttgcacg gcttgaaaaa aaagggttta tccaaagaga
     3001 tccgacgaaa cctcgtgcaa tcgagttgac aaaacaaggt ttagaaaaga ttgggattcg
     3061 tccaacaact attccagtac ttggcgtcgt aacagctgga gaacccattt tagcagtaga
     3121 ggatgcttca gacttctttc caatccctcc taatctttct tccgaagaag gcagtctctt
     3181 tatgctgaca attcgtggag atagtatgat caatgctggg atcttggatg gtgacaacgt
     3241 gatcgttcgg aagcaagaaa ctgctcaaaa tggcgatatt gtcattgcaa tgactgccga
     3301 ggacgaagcg acttgcaaac gattctataa agaaaaaaac tttttccgtc tgcagcctga
     3361 aaacgacttg ctcgaaccaa tcatcttaga tcaagtatct atattaggac gagtagtagg
     3421 actttatcgt agtcatatct attaattgct actaataaaa gaaaactttt taaaaagagt
     3481 ttgagacgat ggtctcaaac tcttttttcg cccgggttct tcttctttca aatttttatc
     3541 acttccctct ttacaaaccg aacaaatatt cgtatactaa atatacgaac aattgttcgc
     3601 ataaattaac gaggtgagaa ttcatgaaat caatgagacg tcttggctta gtattaactg
     3661 ttttgttttt aggtattatt attggtaatt tcggcatacg tccagcttta cttgtgtttg
     3721 ttccgttatt tactttgtgg ttcatgttgt gggatgaaaa gaagtaccgt caatctgagc
     3781 ggcgcagaat tcatgaagat catttctatc atgagcccta ctacagatcc aggcaatcat
     3841 aataaggatt gactgctaat tattcaaaag acaagaagtt aaacttttac gggggtgtga
     3901 caaaaatcgg tcacatcctt ttttcgaata aacggtgttc taaagccagt ttttcggtat
     3961 taagtcaaat tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat
     4021 ttataaaaaa atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt
     4081 taaaatcgct tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca
     4141 ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga
     4201 tcaaaaatgc gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt
     4261 tgattgttcc tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa
     4321 tggcatcctt atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga
     4381 tggtttctcg aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat
     4441 aagcaacagg acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc
     4501 gtttttcttt tgcataattt acgatttgct tcgtagcttc ccgataagcg tcaccatctg
     4561 ccatcagtgg ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg
     4621 caatataatc ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg
     4681 gtaggaatat cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgccgctgg
     4741 tataatctgc tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg
     4801 ttctctattg taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt
     4861 tcagggatac gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct
     4921 tgagaatgga tcagtttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa
     4981 taggcctcat caggcgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa
     5041 acttctttca gatccgctaa atcatctgga caatccataa taactagaga gttcggtttt
     5101 cttttttcaa ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg
     5161 ataatctcag gcacgaattt caacgtagaa gaatcaaatg ctaaataaag gctaccttcc
     5221 gacgttcggc tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc
     5281 acagaaaaat cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa
     5341 agctttccta ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca
     5401 aaagcgaccg catcaagctg actcgatgca ttggaaagga caaatttcag atgctgttgg
     5461 tttgctccga ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa
     5521 ggattatccg taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact
     5581 tcatttggca gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga
     5641 tgatcgacta tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc
     5701 gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca
     5761 agcatttcaa aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct
     5821 tcttttaaag taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca
     5881 atgcccaaaa cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta
     5941 ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg
     6001 atctcattca atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac
     6061 gagacggcag gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa
     6121 aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc
     6181 tgaagtccta tccgttctga atgatgaatg gcattgattc ctaatgaaac aaggattcga
     6241 ttctcatcag tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat
     6301 tctacaggcg gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc
     6361 aaatctggaa atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct
     6421 ggtaaattct caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga
     6481 gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt
     6541 ccttcttcga tcttttcttg gtatacagat tggtttggtc catacccatg ctcaaatcga
     6601 tttggcagga atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt
     6661 gcactggtga tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt
     6721 acagcttctt ggattcggct gattgcttta tccatatcgt gcatcagata cggatcatgt
     6781 aattcttgtt cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt
     6841 tgccacagta acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag
     6901 gattcagaag gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca
     6961 ctcctaactt agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa
     7021 aaacagatca gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt
     7081 atgaaatatc cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt
     7141 tcttccggcg ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa
     7201 gcagcttcca actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct
     7261 tcaattttct tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt
     7321 tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc
     7381 agcgcagaac cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt
     7441 acaggaacat ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat
     7501 ccaagaatca cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg
     7561 ctggctaaat aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa
     7621 ataaacggtt gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc
     7681 aacttggcag gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca
     7741 gccttatcga agaaatctgt ctcgattgga cctggattca ctgtcgtaac agctacgcca
     7801 agtggtttca attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca
     7861 gaataaaccg ttgattttgc agttgccatt tttccagcca tagacgcgat attaatgata
     7921 tgaccttgtc ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct
     7981 aagatgttga cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg
     8041 aaaatcccaa aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc
     8101 ttttcatata attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc
     8161 tctttgccgc tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt
     8221 gcgcaactta taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga
     8281 ccgcctgaac tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct
     8341 cgcgaaatgg aatctcaatg ctatcaaaat ctttcatgat cgtcgtgttt tcaaacactt
     8401 cttgtgcttc tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag
     8461 taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg
     8521 aatggaaata gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca
     8581 gaatatcgct attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa
     8641 tagtcacgat tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc
     8701 catcttctag ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa
     8761 tacctaattc ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac
     8821 gataaccaaa actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat
     8881 cagtaaaaat cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg
     8941 tttgcgaaat acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct
     9001 ctaatggcgt atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa
     9061 tatgatcgcc gtgtaaatgt gtaataaaaa ttttaccgat ttttctcgga cgaatagtcg
     9121 ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct
     9181 cgtctaaaag tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac
     9241 ctgttcctaa aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt
     9301 ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa
     9361 cgttctaaag cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt
     9421 gagatcctct gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt
     9481 ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc
     9541 tttcgcatca tgcacatctt tttcgacatc ttcataatcc aagcgggtaa tttctccgcc
     9601 taattccatc attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat
     9661 cagtcctgta gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc
     9721 ttttatttct ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa
     9781 aatccctagc ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga
     9841 tttgttatta ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg
     9901 atcatggctg acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt
     9961 gatttctgaa aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg
    10021 tttgctcatt aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgta
    10081 aaataagaaa agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta
    10141 tcacagctcc tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct
    10201 cccttaaatc tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa
    10261 attcaaattc aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa
    10321 gagcttcatc cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat
    10381 gatcgaaatt cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg
    10441 catctgaatc acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag
    10501 tttcttcttc cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg
    10561 ctgtagcatt caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg
    10621 ggatgtcatc agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta
    10681 gattttccgg tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta
    10741 gcaagcgtaa gttataagta gataattctt tgttgatggc aagatagtct tcataaggat
    10801 ctcgtccttc catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat
    10861 gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca
    10921 agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttagggacga
    10981 gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag
    11041 tcgatttccc gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta
    11101 gttcgatttt tcgctcttgt cctggttcgc cgttttcagc aatttccggt gcaggattcc
    11161 gaggagaggc aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg
    11221 tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg
    11281 ttccttgtgg tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca
    11341 tcccattttc acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac
    11401 gtaacccttc atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg
    11461 gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc
    11521 cagctttcac atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt
    11581 attcatttat ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta
    11641 tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt
    11701 ttatcatttg gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa
    11761 tattctttgc gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac
    11821 agcaccccta ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt
    11881 gggcgtattg gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta
    11941 gtagcaatca acgacttaac aagtccaact atgttagcac aattattgca atttgactct
    12001 actcatggta cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc
    12061 gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac
    12121 ggtgtagata tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg
    12181 catttagatg ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc
    12241 atcgtctata acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt
    12301 tcatgtacaa caaactgttt agcacctatg gcttatttct taaataatga atttgggatc
    12361 gaagttggta caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga
    12421 cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat
    12481 tctactggtg ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa
    12541 ggacacgcac aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg
    12601 aaaacaaaag taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat
    12661 ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa
    12721 ggttcgatct ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt
    12781 aaaactgttg catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta
    12841 gaaaaattcg ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat
    12901 ccagtcaaag gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg
    12961 gcttaaaaat tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc
    13021 atgctcggct ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt
    13081 atctaacatt tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg
    13141 ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac
    13201 ttttgtcact agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc
    13261 tagctgataa gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt
    13321 cacattatct ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac
    13381 accgtataga acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg
    13441 tacatagtct ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg
    13501 tcctcttgca tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt
    13561 ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt
    13621 cgtttcagca ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca
    13681 atcgcttcgg ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg
    13741 tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt
    13801 ataaaaataa ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag
    13861 gattttcatc caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg
    13921 aattagtata atcaaagaat ctctactatg caatgttcat ttttataac
//
LOCUS       DACDRN010000060        13950 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDRN010000060 DACDRN010000000
VERSION     DACDRN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.060"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            950..1423
                     /locus_tag="IWJ80_002100"
     CDS             950..1423
                     /locus_tag="IWJ80_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP9461806.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1432..1659
                     /locus_tag="IWJ80_002101"
     CDS             1432..1659
                     /locus_tag="IWJ80_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9461807.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2295..2666
                     /locus_tag="IWJ80_002102"
     CDS             2295..2666
                     /locus_tag="IWJ80_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461808.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2922..3164)
                     /locus_tag="IWJ80_002103"
     CDS             complement(2922..3164)
                     /locus_tag="IWJ80_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9461809.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3196..4098)
                     /locus_tag="IWJ80_002104"
     CDS             complement(3196..4098)
                     /locus_tag="IWJ80_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9461810.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4111..4299)
                     /locus_tag="IWJ80_002105"
     CDS             complement(4111..4299)
                     /locus_tag="IWJ80_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9461811.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="IWJ80_002106"
     CDS             complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="IWJ80_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9461812.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4914..5807)
                     /locus_tag="IWJ80_002107"
     CDS             complement(4914..5807)
                     /locus_tag="IWJ80_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9461813.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5885..6823)
                     /locus_tag="IWJ80_002108"
     CDS             complement(5885..6823)
                     /locus_tag="IWJ80_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9461814.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6857..7207)
                     /locus_tag="IWJ80_002109"
     CDS             complement(6857..7207)
                     /locus_tag="IWJ80_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9461815.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7240..8142)
                     /locus_tag="IWJ80_002110"
     CDS             complement(7240..8142)
                     /locus_tag="IWJ80_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9461816.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8135..8992)
                     /locus_tag="IWJ80_002111"
     CDS             complement(8135..8992)
                     /locus_tag="IWJ80_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9461817.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9326..10135
                     /locus_tag="IWJ80_002112"
     CDS             9326..10135
                     /locus_tag="IWJ80_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9461818.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10175..10672
                     /locus_tag="IWJ80_002113"
     CDS             10175..10672
                     /locus_tag="IWJ80_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9461819.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11057..11130)
                     /locus_tag="IWJ80_002114"
     tRNA            complement(11057..11130)
                     /locus_tag="IWJ80_002114"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11094..11096),aa:Ile,seq:cat)
     gene            11317..11640
                     /locus_tag="IWJ80_002115"
     CDS             11317..11640
                     /locus_tag="IWJ80_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461820.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11804..12058
                     /locus_tag="IWJ80_002116"
     CDS             11804..12058
                     /locus_tag="IWJ80_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461821.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12128..12373
                     /locus_tag="IWJ80_002117"
     CDS             12128..12373
                     /locus_tag="IWJ80_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9461822.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12449..12814
                     /locus_tag="IWJ80_002118"
     CDS             12449..12814
                     /locus_tag="IWJ80_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461823.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12875..13438)
                     /locus_tag="IWJ80_002119"
     CDS             complement(12875..13438)
                     /locus_tag="IWJ80_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461824.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         4090 a         2577 c         2304 g         4979 t
ORIGIN      
        1 tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc gttcttactt
       61 tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc ttataaataa
      121 aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa cgattggcta
      181 tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat tcagttggac
      241 tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac aaaattatcg
      301 aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta tggtactttt
      361 tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac ttttgattta
      421 taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga tagtaccacc
      481 ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata aaggacaaat
      541 aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa taaacatgaa
      601 cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct catacagttc
      661 tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta ttgtccaata
      721 taatcccagg catctaaact agcattatag aagagacaca catagaatct aaaataactg
      781 gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac cttcatactc
      841 tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag ttgttttaca
      901 ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa tgaaatttaa
      961 aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt tgttcggcgt
     1021 acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt ggcagaccac
     1081 attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg cttatttctt
     1141 attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa gcttaaacat
     1201 actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg gcttacttcc
     1261 taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg taggaatcgc
     1321 tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa aactgcttga
     1381 acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga catggcaatt
     1441 atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga attgagtgag
     1501 aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc aaaagccatc
     1561 cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg tgatttgttg
     1621 acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga acaaacagta
     1681 aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt acgtctatta
     1741 ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat gtactgactc
     1801 atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca actggtttcc
     1861 tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca ccagactgat
     1921 agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt tcataccttg
     1981 aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat tcttttttag
     2041 taagctccca tatatactgt aaagccttct atatattatg tcatgttgct ttttaacatt
     2101 tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct tttttatttt
     2161 taacattatt ttgataataa tgattattta tttaaaaata cattagataa catttgttac
     2221 gacacgaaaa agcttcacaa acaaatatcg ttattttaaa atgagggcga actcacacaa
     2281 ttaggaggaa tagtatgcca tttattgttt acttttttct ttcattactc gtgatctata
     2341 ttccattacc aacatttttc ctgctcgtca atcgtctagc agagcaacat gatacgattg
     2401 ctgctcttac gaaactttgt ttacttctat ttgtttgtct agattacttt acgagctatt
     2461 attggctaaa acagacaaga gtaaaaaaga gatattattt cttccttgtc tttttcaatc
     2521 tctttgagtt tctgatccat tgctttttac ttcatcagta tcatacgatc catttgtgga
     2581 tcatctgggt tttcaaagtg gtcgtattat tttgtatgtt cacctttcct atctcaagaa
     2641 aatttaggga ggcattgttt tattaaagaa gcttctctgt atcaataaag acacatttaa
     2701 ggaaaaactt ttaaaaatct aattgggaat ttgcccgcat aaataagagg ctgtgacaaa
     2761 agtcttgtca cagcctctta ttccgtataa acagtgttca aaagcttgca tcttcgataa
     2821 tatgcccaac caaatgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg
     2881 atgaagaaca gctttttcac gacctctttt taaaagtctt tttacagttt tcgagcaaag
     2941 aatgatacga ttagtactaa aatgatggca ccaatcaatg atggcacgat tgccattcct
     3001 gcaatacttg gtccccaaga acctaataat gcttgaccaa gtgctgatcc aactaaacct
     3061 gctacgacat ttgcaataat ccccatcgaa gaacctttat tcgtgattgc tcctgcaatt
     3121 gctccgataa ttcctccgac gataagtgac caaataaatc ccatgtttcg ttcctcctaa
     3181 tgttttactt ccttattact gaacccgtgg ttctgtattt tcttttactt tttgtgcgcc
     3241 gcttccagct gctgatttta ctttatctgt ttgattcgaa gcaaattcac cggttttttt
     3301 cgctgcgtca gttactttgt cttgcaccgt ttcttgatct ttttcaaatt cttcttttgt
     3361 tttgatgtcc actacgttag cgttgacttc gatcacttct aaatgtgtca tattttttac
     3421 ttctttgccg attacttctt gcatttcttc aaagattttt tcgatatctt tgccatattc
     3481 cacaatgaca tccatatcaa ctgctacttg ttttttaccg acttctgttt cgatcccagc
     3541 cgttacatta tctgtattca caagttttcc tgccacatta gagaagaaac caccgtttgc
     3601 agataataaa ccgtctactt gttcaattgc gattccgatg attttttgca caactttatc
     3661 ttcataattc agctcaccgc gcattgaatc ttcctgttca gaatcatgct ttccttggtt
     3721 atttgtatga tcagaaccaa gttttccttg tccgtttgtg tggtcagaac taagtttttc
     3781 ttgtgctttc gcatggtcag aaggatgttt ttgctcttcc acaggtttag aaggatcgat
     3841 tgcatcatgt tgttctggaa ctttagattc tggagttcct ggatgatgag gtgcatgctt
     3901 ttctgcatca tgtttcttct cttctactcc ttgttgaacg ttagggacta ctggatctgg
     3961 atgtgcagat actggtgctg ttttgttctt ttcatcgttc acaccatttg attggtttgg
     4021 tgtcgttggg tctggatgtg cagacactgg tttttggttg ttcattttat tgttattatt
     4081 tttgttattg ttattcatgg atgtttcctc ctaagattta aaatagtgat ctaagatttt
     4141 cgtttgtttc agataaaaac cgatagcgac gccgatcgct gccatgatca agatcaataa
     4201 ggtcttgaaa aagcccacgg tgatccataa aacggctaat atcaatccaa gtaccccaaa
     4261 gatgataggg agcttgtatg tcgaaaatag ttctttcatt tttggtcccc ccctattcta
     4321 ctcgtttgcg gtcattgctt cgtttagctg gttcttcaaa gcctgtgtat ttcaccgata
     4381 ctttcactgg ctcttttgtt cccaatatgt gttgtacttc attttcgatt tctttcatca
     4441 atgttcctgt tttaccaatg agtgctgaag ttcgtttcaa ctcccctttt acattgacgg
     4501 cgatcttgtt tttcgttgct ttgacattta cttttggtga atggatgaat tcatcttcat
     4561 gcaagtgaga acgaaccatt ccttcaattg cttttttatc caacgtcaac tccccatcac
     4621 catgtttcag tagaaagttt ccgattgttt ttggataaaa caggaaaaca agtaacgtga
     4681 taattgtcaa aatagcaaat gcaacggcaa gccaaaatac atattgttgc acgtatgaat
     4741 tagtgatgga aaagaatcga aacttctcta gctgaaaagg caatggatag gttgcactta
     4801 atagtatcgc aaacaaaagc acagatagca aaagtaatgt tccgatcact ccgatcgttt
     4861 ttacaccttt attcattttc gcacctcctc ataggggtga aacgatcaac ttcttataag
     4921 ttgatcgctt ctccaccagt tactccgtaa atttgggctg taacataact tgctttattc
     4981 gatgctaaga aaacataaac tggtgctaat tctcctggct gacctgcacg ttctagtaaa
     5041 ctttgctgac caaattcagg gatcgaatca gaaggttgtc cttcgtctag ttgtaatggt
     5101 gtccagattg gaccaggtgc tacaccattt acacggattc ctttaggtgc aagctgtttt
     5161 gctaaaccga tcgtaaagtt agcgattgaa gcttttgttg ctgcgtaatc taataaatgt
     5221 tcgcttggat tgaatgcttg aacagaagtt gttgttacga ttgctcctcc tgctgggata
     5281 tgcggaacag cttctttcac taaagcaaac atagcgatga tgtttatcat aaatgtgtct
     5341 ttgacttgct caagtggtaa ctcagcaata gactgacaag aaatttgctg tcctgcgttt
     5401 agtaccatcg tatctagccc gccaagttca ctcacggctt tatctatgat ttctttaggt
     5461 gcttgttcgt cacgaagatc aaaaggtagt aagacaatct tacgtcctgc aactttcacg
     5521 tattttgcta cttcctctgc atctttttct tctcctggga agaattgaag agcgatatct
     5581 gccccttctc tagcaaatgc aatcgctgct gcacgaccga tccctgaatc tccaccagtg
     5641 attagtacac ggcggttttc tagttgatta ttgccttcat aggattcttc tccacaatca
     5701 ggaacaggaa tcatattctg ctgtaaagct ggtgtatcct gatcttgttt aggaaatttc
     5761 tccgtatggt acaagtttcg cgggtcgatt aattttggtt ctgtcattgc tgtattcctc
     5821 ctagttttta aattgtattg ccaaaacaaa tttctttgtt tgatacctgt tttaaataga
     5881 ttctttaatt tgtataatct ttccggtata aatggtaacc taaaactact gctgctatca
     5941 aagagccaaa gactacgagt ataaagccta gagcagaact tttacccggt aatcctcctg
     6001 tattcattcc ccaaatccct gcgaacaacg ccggaacgga aatgattaat gcagctgaat
     6061 ctagatattt catgatatga tttagattat tgtccatcat cccgttgaat agatcaccaa
     6121 tactctctaa taaatctcga tagatctcga tcattttttc tgtttgtcgc tgtctcaaat
     6181 ggacatcata gagtagctgt tcatcgtcta aacgtttgat gaaagtttca gaattccata
     6241 attgatccat tgtttcccgt tgatctctta aggtatgatc gatatatacg atgtctcttt
     6301 caagatctgc ctgtttatac agttcttcat tttccgttgt tttacgagct gctttatcca
     6361 aagcgtcaat tcgctttttc atctctctta gttctttcac gtaatgggtg taaatatcta
     6421 ataatgtata cgtgatgatc ttttcaaacg agtttaaatg atttccatgc tttgtcagca
     6481 gccgatccac aaattcggtt tttctatcga tacaagtaat caagagatta tcggaaagta
     6541 caaaagatat tggcgttaat tttttttcga tacgattatc tgactgatct agattaatca
     6601 ctaccaaaga aaatggatgc tctagtttcg tctcataaag gcgctccatc cgagaaactt
     6661 cttccggata ttcggttcct acgaaaatat catcaggaag ctcgaattct tcgattgctt
     6721 gctcaatctc ttcgggcttt gcactatcaa gtacgagcca attatatttt ttttcatctg
     6781 tttctattac attcttttct tcatccacaa agtagtatct caatgtatac ctcctttttg
     6841 gactttctcg aaactattag ctgaagaagc agttctttaa tttgatatta taatcgagta
     6901 ctgcgatttc attttctaat gaaaagctgt caagctgttt gttgaagtcc atgttcaatc
     6961 gtttaccgaa ataattttgc tctaatttag atgtatagat gtccaataat ggatgctcga
     7021 ttttagtcgc atattctcca aattccgaaa caaaaggaat gatttgaaaa aagctgcctt
     7081 ttgcaaaagc acctttgtcc cttagaaatg gatcggaatc attgataagc gcgagtgcga
     7141 tttttgtatt ttccccaacc ttttcaatta acgtctctac tgcattttta gacattgtaa
     7201 tttccattcc tactccccct cttcagttgt tgtcttctat tatttaacaa ctacatctgt
     7261 ttttggttga acttttgcat attttggata tttagggaat ttagctacac aacggaaagc
     7321 cgtcatccct gcatattttt cgtaatattt cttcgttggc ccttcaaaat ccttgctcca
     7381 cggtttcaca tgtcctgaaa aatggataat gtaaggattg ttgcgagttt cttcgtattc
     7441 acgttcacct gctgctgttg gatgtttttt tgcttttgcc atgatatagc cttgtgcatt
     7501 ccaacgcgga tgaagtggta accaacggtc atgtaagata gcatttaatg catcttggtc
     7561 atggaatcgt aatttttctg gattatgttc gatgaaatct aatacttttt gcgtaatatt
     7621 ttcatctaac cattttttca cattgatcag catcaaacct gagttgaaat aacgcattga
     7681 tttagctgaa attttcattt tttccagtcg ttgatggaac ccagcgtctt caacagctgc
     7741 tacgattgaa tcaccgaagt caagacgcca taatttagag atatcttgta aagcgatcat
     7801 gtcgcaatcc atatataaga tacgttcaac ttctgtgccg cggaacaatt ccggaatcgc
     7861 aatacgataa taagctgttt ctggaatacg atcgctgatt accacatttg taaagaattc
     7921 tttattgatt ttcaagaatt ccacatctga gttcattcgt gcgtttttca ctgagaagcg
     7981 taaaagctgt ttactttcaa aatcgatatc atcgtcaatc acatagaacc gaacaaattt
     8041 agacgggttg caattatcta atactgagac aaataatgca gctaaatgtg gtacgaattt
     8101 tgtgttacaa cttgcgacta ctgctatttc ttttttattc attgatctcg cctactttcg
     8161 ttagagtact gtgtgctaat ttttttaagt ataaattggt atacggatga tctttcaacg
     8221 tattccaagg tttatcatgt cctgtaaagt gaacgatcga aggtttttca tttccttctt
     8281 tatactgacg ttcatatttt tcatttggtg ctggatgacg ttcaaaaatc agcgaagtct
     8341 gcatgttcca ttttggatga agttgttccc aatcttcata aagtacagca ttcaaagcat
     8401 cttggtcgtg atagatgatt cgatcgccat tttcactgag gtaatggata gttttttctg
     8461 taatttcttt tttattccat tgatcaatgt caatcaccat cactcctgaa ttgaagtaca
     8521 agtcatcgga atcgattcct aatctttcca aagctttcac ttggcctgga tcgataatag
     8581 cgccgattgt ttttccattt aacggctcat catatagttt tacgatatcg tctagtacta
     8641 acacatctga atctaagtaa aggactttct tataatcctc tttagctaat aagtttggta
     8701 aagaaattct taagtaagcg gtagttgtga tatggtcact gactaggaag tcttcgtaga
     8761 tatctttttc cactgtcagg aattggatac ttgcattact tgaatattta tttaccgttt
     8821 cttccaatcc ttctttacta tattctgata gaccatcgtc aatcacataa aattttatgc
     8881 gtcttgtttt attgcaattt tctaatgcag ttgcgatcat cacgctcaag taaggagcat
     8941 aattttcgtc tgaagcagtt accacaggaa ctacgccgta tctcttctcc atcaaaatca
     9001 ctcctttttc taatcttttt ctaacctgtt ctacatctag aatattcctc atttggtttt
     9061 aaagcaaaat atatgctttg ttttcattga atattttaaa atttttttat cacaaaacgc
     9121 atcggtaagc gctaactatt cgtttttcat ggctttttat tcaaaggtat tgaccaaaaa
     9181 ctactatata tagccacctt tttcttaaga atgttttttg aaaaaattca taatgacaaa
     9241 gcaagttagt ttcatttttt taaattctca tttaatctta aggtaacagg aaaatagata
     9301 tgtaaaaaga aagtaggaag ataaaatgaa actagcagcg attgatttag acggtacctt
     9361 gcttgatcga aatggaaaga taccagaaga aaatatccaa gcacttcgga aatttgatga
     9421 aaatggcgga atcgtaaccg tcgctaccgg aaggaacagc atttctgcta aagatatttt
     9481 ttccgaatta ggaatcggtg gctatttgat ttcttccaat ggttcactga ttgctgaaat
     9541 gaaggatgga aaaatggcga aagttctcag acgttcaaaa atagatagcg atacactgag
     9601 aaaagccttc ttctatgctc aagaagcgaa aatatccatc attgctagcc gagaaaccca
     9661 agatgatcag ctgacattcg atgaatcaaa tctagtcaaa gatgaccctt attatgaaca
     9721 tttcaattta cagaaccatt cttttgatca aattttaaat atgttggaag atccttctct
     9781 tagctatctt aaattagcat taacagataa gagtgaagaa aaactacaga agctacaaag
     9841 agatctgcaa gcagatggta tccaatcagt attctctgat ccgcatttct tagaaatcac
     9901 gcctacaggt atcactaaag ctcattcact tgtttttctg gcggattacc ttggaatcga
     9961 atcgagtgac atcatggctt ttggcgatca agaaaacgac attgctatgt tgaattacag
    10021 cgggttgagt attgcgatgg ggaatgcaca gcgacaagta aaagaaattg caaaccaagt
    10081 aacactgacg aatgaagaag ctggtgttgc ttatatttta aaaaacttct attaaaaaga
    10141 ataaaatgac gatatgaatg taggaggaaa gaaaatgtta gttattattg atatgcaaaa
    10201 tcatattctt gatccaaaca gcgaatttta tattccagag tcagatcaat tggcagaacg
    10261 tattgctcaa agattagcta aagcacgcca aaataatgaa tatattctct tcacacggga
    10321 tataccagtt gaactgaagg acaaagaaga agaacgagaa gatttgcaat tgatccctct
    10381 tctttctcca caagaaaatg aacgagtgat taaaaagtat tactttacga ttccacctga
    10441 aactttagct gaaatccagg aaactttatt tgaaagcaaa gaagaacaaa aagagatcga
    10501 agtagtcggg attgaaacaa acttgtgtgt actctctaat ctgataggcc tgcagagtgc
    10561 ttttcctgaa gcagactttt ttgttgatcc aatgttagtc tccagtagaa aacacggaga
    10621 atccgcttta gagctgttga aagatttcaa cgtctctatc gtcgaatcat aaaaatatct
    10681 atcttcattt acgatattta acacacaata aaaaaagcac gcagaactgt tttagtccta
    10741 cgtgcttttt ctatcaaagt agattggctt ttttatgaca cagagtgaaa cggcaggctc
    10801 acgacctctt tcttaaatgg tgttcaatca gctgaccctc ggtattagct taacaaacgg
    10861 caaaatatga ggaactattt ttccgtttgt taagctaata ctcaggtcaa aacgctgatt
    10921 tcacaacctc ttccttaaac ggtattcaaa agcttgcatc tgcagtaata agaacagcca
    10981 agtaaaaaca taagaagctg tttttacttg gctgttctta ttacttgatg cagggcagct
    11041 ttttcacaac ctcttatgta ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
    11101 gtctgctcta accaactgag ctaaaggtac atcataaata aaatttattt ttgttacttt
    11161 tcaactatag cacgaatgac aaactctcac aagtttcaaa atataagtaa aataatattg
    11221 ttattcttat ttcttttact tgtgtcataa ctattttgtt actttaattt ttatttgctt
    11281 attttagaaa aaatgttatt cttttattta ttctcaatgt taggagtgat tattttgcca
    11341 tttattgcga ttttattcga tttagttgcg gcagcagctt attttctcca attaaattat
    11401 caaacctcag gaattctgtt tattggaatg atttttcaag gtgtgattac ccttttgctt
    11461 cttgtcatga tgctcagcta taaaggaaaa agatatgccc gtattcaaac aaaagtattt
    11521 gttaaatatg tttccattcg ctatgggatc atcattcttt cctttttagt gaatgcgatc
    11581 gtcctctttt tatatgtatt gaattactta gatatcaatc cattgatttt ttctcgatag
    11641 ataagacaca ttcagcggaa cagttcctgc ttatcagcag aaaactgttc tgctttttca
    11701 tttttcattg atctattctt cttttatttt cctagcattt catttgtgat gtatgcaata
    11761 acatgttatc ttttattata tgaatcgttg aggagtccgt tacatgatta cgttggtgag
    11821 agttttgttt tgggtgcctg cggtcgcact ggtcgcaagt attgtttatc ttatgaattg
    11881 gaacaaagaa cggttttatt tagctatttt gactcttccg gctatctact ttatgtggaa
    11941 agtttttaat tacaactact ttgaacccga tagtgttttt atagaagaat tatcaggact
    12001 ggttttgtct ttattgatcg tcattcttta tttgatacgt ttaaataaaa aacattgagt
    12061 actattgatt gcatgtgtag ctttctatag tcattataac tattctacta taatgaaagg
    12121 tgattaaatg agtatcgaag aagctatttt actggatact ttacgttgtc gtctgaattt
    12181 cttaagaact ggccgatctt tatctcaagt agctgtttct agcgcgttaa gtattccttt
    12241 attcaaatat cgtaagtacg aagaaggaga agaaattcca agcatcattg aattaatcta
    12301 tatcgctgac ttttacgata tttccgttga ttacctaatt ggtcgctcag aaacagcaag
    12361 caaactgaag tgattttttc cagccttggt gctggttttt tcatttaacg tctaaggtta
    12421 acaatctata tataaaggag acatttctat gcgattatgg catgaaaaat tgatttcaaa
    12481 gcttcctcgt caacagcttt taggacaaca tcgggaatgc tgtgcattaa gaggaaatgg
    12541 ctggggaaag aaacatgcta ctgtggatta tgtttttcac tattctcctt atagattatt
    12601 ccaatatcat cagcttgtca tggatgaaat ggaaaaacgt ggttacagcc cagcaccaga
    12661 gtggaaaaat cctatgtatc gtggcaaatc atgcgaagca ttttttgatt tacctactgt
    12721 agagcgcaac gtgccgattt atccggaaca cgatgaagtt tatctagcag aatgtttagc
    12781 aaatttggag caaaaaggaa tttatcttac ctaatataac tagaaaagcc ttctatctcc
    12841 tcttacttca ttaaaaagct ttaaccatct tagatcaagc taaaatgtcg taagttttag
    12901 ggttatcttc atcctcacgt gcaaaactaa aagcgtactc tagttttggt tcatcgccaa
    12961 cttccctaga cataaatgaa catctcctga cgtggtttaa aagtaccgca tcttcgacgt
    13021 ctgtatacga tgcacagcac agattgcttc ttttgatctt tcttttgatt tttctgttgc
    13081 ttcttctaca ccttcttcga ttactacatc gatgcttaca attgcttctg cctaatattc
    13141 ccatgcatcg tcgatttaag cctctgtagt cactgtgatc gtttccacat tttaactcct
    13201 ctgtcgtggc ttcagttgat tttgtgatca gttcagttgc taagccagag tcagaacaac
    13261 ctgccattgc tagcgttaat attgatagta aaaatagttt ttttatcata aggttccccc
    13321 cctgaaaata gactaaaata ataacgcttt ctttttttgt tttcagacga aaattaataa
    13381 tttctaaata ttctttaaaa aagcttataa ctattatgcc ttttttaata tataacacag
    13441 ttatttttaa aaataagaat gctaaatcac tctacaataa cggcgaaagc acaaaaaata
    13501 aaaaaattat tacgttatta aaaaaacaga tgaaagatca ggttttatta gatctcattc
    13561 tttcatcact tgaagggatt taatgaacta ctttttcctc tttgtcgttt ttatatattc
    13621 gtattccttc aaacagtagg atagcctctt tcaatgcttg atatttgaac tgtccgttac
    13681 ttaaaaattt agatgtgccg ccctcttcct agaacatatt cttttttact ttttcattaa
    13741 ttgacagacc aatcgaacga aactgtaaat ttcgatagga taatgattgg tcaaactcat
    13801 tcattttcaa gacatctcga ttcatattca agataggaat ctttttatca acatcacttt
    13861 attcgtattt tcctctacca taaaagcttc acgctccaca aaattgtttt atacactact
    13921 attcagccca ttcaacgacc aatgaatttt
//
LOCUS       DACDRN010000061        13721 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDRN010000061 DACDRN010000000
VERSION     DACDRN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.061"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IWJ80_002120"
     CDS             complement(<1..914)
                     /locus_tag="IWJ80_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP9461825.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IWJ80_002121"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IWJ80_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP9461826.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="IWJ80_002122"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="IWJ80_002122"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP9461827.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="IWJ80_002123"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="IWJ80_002123"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP9461828.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="IWJ80_002124"
     CDS             4440..5321
                     /locus_tag="IWJ80_002124"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP9461829.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="IWJ80_002125"
     CDS             5318..6034
                     /locus_tag="IWJ80_002125"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP9461830.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="IWJ80_002126"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="IWJ80_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP9461831.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="IWJ80_002127"
     CDS             6751..7752
                     /locus_tag="IWJ80_002127"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP9461832.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="IWJ80_002128"
     CDS             7749..9110
                     /locus_tag="IWJ80_002128"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP9461833.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="IWJ80_002129"
     CDS             9070..10221
                     /locus_tag="IWJ80_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP9461834.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="IWJ80_002130"
     CDS             10196..11365
                     /locus_tag="IWJ80_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9461835.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="IWJ80_002131"
     CDS             11540..12919
                     /locus_tag="IWJ80_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9461836.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13721
                     /locus_tag="IWJ80_002132"
                     /pseudo
     CDS             13200..13721
                     /locus_tag="IWJ80_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         4575 a         2398 c         2984 g         3764 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagatta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagtt ggagcagtag
    13441 ccaaaaatcg gatccatcgt cgtttctaca ccagtatctg ccatcagaaa atcacgacgg
    13501 atacaactga atttaaatac catgaagtgg atgccaaagg cattatccgt caaaagaaat
    13561 tgtatctaga tccttttatg gatatgttca atagtgagat tttatcctat cgtatctctg
    13621 aaaagcccaa tgcattagcg gtaatggaag gtttggagga agctatccag accactaacg
    13681 attgtcccta tcgtcgtacg tttcactcag atcaaggttg g
//
LOCUS       DACDRN010000062        13679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDRN010000062 DACDRN010000000
VERSION     DACDRN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.062"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..902)
                     /locus_tag="IWJ80_002133"
     CDS             complement(<1..902)
                     /locus_tag="IWJ80_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP9461837.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="IWJ80_002134"
     CDS             1185..2153
                     /locus_tag="IWJ80_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9461838.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="IWJ80_002135"
     CDS             2266..2544
                     /locus_tag="IWJ80_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9461839.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="IWJ80_002136"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="IWJ80_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP9461840.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3010..3756
                     /locus_tag="IWJ80_002137"
     CDS             3010..3756
                     /locus_tag="IWJ80_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9461841.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="IWJ80_002138"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="IWJ80_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP9461842.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="IWJ80_002139"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="IWJ80_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP9461843.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="IWJ80_002140"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="IWJ80_002140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP9461844.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="IWJ80_002141"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="IWJ80_002141"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP9461845.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="IWJ80_002142"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="IWJ80_002142"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP9461846.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="IWJ80_002143"
     CDS             8493..9035
                     /locus_tag="IWJ80_002143"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP9461847.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /locus_tag="IWJ80_002144"
     CDS             9058..10614
                     /locus_tag="IWJ80_002144"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP9461848.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="IWJ80_002145"
     CDS             10628..11530
                     /locus_tag="IWJ80_002145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP9461849.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRAVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="IWJ80_002146"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="IWJ80_002146"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP9461850.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="IWJ80_002147"
     CDS             12973..13395
                     /locus_tag="IWJ80_002147"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP9461851.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4521 a         2448 c         2980 g         3730 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca
     9901 ggtcgtgaag cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgcctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaagta attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgctgctc agccgtgctg tgaagaaaac tggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg ttccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattacaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggggaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca
    13561 gaggttgtaa aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttattt
//
LOCUS       DACDRN010000063        13147 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDRN010000063 DACDRN010000000
VERSION     DACDRN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.063"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(77..149)
                     /locus_tag="IWJ80_002148"
     tRNA            complement(77..149)
                     /locus_tag="IWJ80_002148"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IWJ80_002149"
     CDS             530..1099
                     /locus_tag="IWJ80_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP9461852.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IWJ80_002150"
     CDS             1086..1946
                     /locus_tag="IWJ80_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9461853.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IWJ80_002151"
     CDS             2136..2840
                     /locus_tag="IWJ80_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9461854.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IWJ80_002152"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IWJ80_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP9461855.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IWJ80_002153"
     CDS             4677..5993
                     /locus_tag="IWJ80_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461856.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IWJ80_002154"
     CDS             5994..6863
                     /locus_tag="IWJ80_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461857.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IWJ80_002155"
     CDS             6918..7727
                     /locus_tag="IWJ80_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9461858.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IWJ80_002156"
     CDS             complement(7830..8459)
                     /locus_tag="IWJ80_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP9461859.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IWJ80_002157"
     CDS             8629..9921
                     /locus_tag="IWJ80_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9461860.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IWJ80_002158"
     CDS             complement(9997..10659)
                     /locus_tag="IWJ80_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9461861.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="IWJ80_002159"
     CDS             10884..11168
                     /locus_tag="IWJ80_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP9461862.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="IWJ80_002160"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="IWJ80_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP9461863.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4370 a         2265 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccattca
//
LOCUS       DACDRN010000064        13068 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDRN010000064 DACDRN010000000
VERSION     DACDRN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.064"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(65..1174)
                     /gene="rpoD"
                     /locus_tag="IWJ80_002161"
     CDS             complement(65..1174)
                     /gene="rpoD"
                     /locus_tag="IWJ80_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP9461864.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1198..3039)
                     /locus_tag="IWJ80_002162"
     CDS             complement(1198..3039)
                     /locus_tag="IWJ80_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP9461865.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3221..5614)
                     /locus_tag="IWJ80_002163"
     CDS             complement(3221..5614)
                     /locus_tag="IWJ80_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9461866.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5721..6581)
                     /locus_tag="IWJ80_002164"
     CDS             complement(5721..6581)
                     /locus_tag="IWJ80_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP9461867.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6640..7272)
                     /locus_tag="IWJ80_002165"
     CDS             complement(6640..7272)
                     /locus_tag="IWJ80_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP9461868.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            7481..8200
                     /locus_tag="IWJ80_002166"
     CDS             7481..8200
                     /locus_tag="IWJ80_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9461869.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            8226..10817
                     /gene="mprF"
                     /locus_tag="IWJ80_002167"
     CDS             8226..10817
                     /gene="mprF"
                     /locus_tag="IWJ80_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9461870.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11005..12192)
                     /gene="tuf"
                     /locus_tag="IWJ80_002168"
     CDS             complement(11005..12192)
                     /gene="tuf"
                     /locus_tag="IWJ80_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9461871.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12560..12916
                     /locus_tag="IWJ80_002169"
     CDS             12560..12916
                     /locus_tag="IWJ80_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9461872.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3743 a         2733 c         2328 g         4264 t
ORIGIN      
        1 ctttttttct tttaaaataa accaaacagt tttactgatt tctcttctgg tcaatagtcc
       61 acgcttattc taagaaatct tttaattgtt ttgaacgaga aggatggcgc aattttctta
      121 atgctttagc ctcgatctga cgaatacgtt cccgagtaac accaaatact ttccctactt
      181 cttccaatgt tctcgtccgg ccatcatcta aaccaaaacg taagcgaagg acattctctt
      241 cccgatcagt caatgtatct agtacatctt ccaattgttc ttttagtaac tcataagctg
      301 cgtgttctgc tggacttgtt gcttcctggt cttctatgaa atcacctaag tgagagtcat
      361 cttcttcacc gattggcgtt tcaagggaaa caggttcttg cgcgattttc aagatttctc
      421 ggactttttc tgttggaaga tccatttccg caccgatttc ttctggcgta ggttctcttc
      481 caaggtcttg cagcaattga cgttggatac gtatcaattt attaattgtt tcaaccatat
      541 gaactggaat acggatcgta cgtgcttggt cagcgattgc tcgagtgatg gcttgacgaa
      601 tccaccatgt agcataggtt gaaaacttaa atcctttgcg gtagtcaaat ttttcaactg
      661 ccttcatcag tcccatgttt ccttcttgga tcaagtcaag gaactgcatg cctcgtccta
      721 cataacgttt agcgatactt actactaaac gaaggtttgc ttctgccaga cgctgtttcg
      781 cttcttggtc gccttcttcg attttcaatg ctaacgctac ttcttcttcc gcagttaaaa
      841 gggagacacg gccaatttct ttcagataca tacgaaccgg gtcattgatt tttacaccgg
      901 ttggcgcaga aagatcttct gtttttgctt gttcagcttt tttctcagca ctcttcaagc
      961 tgtgaattga tggatcacca ttttcatcca ccacactgat acctgcgtct tcaatctttt
     1021 ggatcaattt gtccatttca tctgcattca aagtgaatgg tgtagcaagc tgattggata
     1081 aatcatcata tacaacttgt cgtttgggtt tgttttcttt gatgaaagct gctactgctg
     1141 cttcatacgt ttttttatct gttccatttg ccataaaaga aggcccccct tgagtagcta
     1201 tttggcttgt tttaactgtt tcgttaaatt gatgatttca atcgtcaatt caagctcaag
     1261 ctgtttgttt ccagttcgtc tggcttcttg ctgcatttgt ttttttaatg ttatttcatc
     1321 agctaagcca gatttgctga tcacatctag caagtcttgg atttctcgtg gcgaactttc
     1381 ttctgccacc ggtaaatatt cgatttccac caccaaccgc cttaaatttt catcttgcaa
     1441 aaagtctaaa aactctgcaa ggttgaattc ataatgttgt gagacatatg catcgaataa
     1501 aaaataaact tctgcatatg catcatgaat aaactgaaat tctcgttctt ttaaaatatt
     1561 acggacagaa acttctttaa atgcacgata taatagaatc tgctctgcct tttctacttg
     1621 cgtcaaaggt ctctttttcc gtgcagtttc ttgtctcgta acctgagtgt gttgactttc
     1681 ctgaggttct tgacgatttt gctgatttgt ccttcgaaga tcgcgcagtt gtttttgtat
     1741 cgtctctctg ccgatctgaa actctgttga cagttgagat agatacatat cttgttcgat
     1801 tggtgaaaca actcggctaa gttcaactag taattcttcg aggtattcga tcttgtcttt
     1861 ttcattatct aaatttttcc cttgttttaa ataacgtgat ttaaaagtaa agactgtttc
     1921 gcgcccatga gccactaatt cttggaaaga tgcttctcca tacttacgga gatattcatc
     1981 aggatcaagc ttttctggaa cgactacgac agaaagtgct aatcgactgt tttgccgaag
     2041 caattcaatc cctcgattgg ttgcttcaaa acctgcgttg tctccgtcat agcaaaagac
     2101 caattctttc gtcagctttt ccatttgttg gatctgttga gctgttagac ttgtccccat
     2161 agaagctaat ccagttttga taccagcctg ccatgcagca atcacatcca taaacccttc
     2221 gaacaacaaa atttctccgt ttttccggat atcgcttctt gctttatcaa gattaaataa
     2281 aacttgtctt ttattgaaca gttctgtttc tggactattc aagtatttgg gctgatcttt
     2341 ttcctgccct ttttcaggtg caagccagcg tcctgaaaaa ccgatcgtct ggccttgaga
     2401 attacgaagt ggaaacatca ctctttgata aaaacgatca gacatacggc catcatcatg
     2461 cgtgacaaac aatccagagc gttctaacaa ttcatttgaa attgattcat tcttgaatac
     2521 ttgctgaaga aaagaacgct cattaggtgc aaaacctatg ttgaattctt caattaattc
     2581 taaagacaac ccacgacttt gcaaatattc taaagctggc tgtccagctt ttgtatgcaa
     2641 tagcatgtga tgatagattt ccgctgcttt ttcatgagca gcaatcagtt tccccgtagc
     2701 agattcagga ttttggacag ctatagtacg ctgacgccat tgatcttcta acggtatctg
     2761 ttcgaagtct gccactttta ccactgcttc tggaaaactt agaccttcta gttcttgaag
     2821 aaaagtaaac acgtttccgc ctttaccaca accaaagcaa tgaaaaatct gcttgtcttc
     2881 tgcaacagaa aaagacggtg tacgttcttc atgaaaagga cacagcccca agtaattttt
     2941 accagacttt ttcaactgga catattgtcc aatcacctca acgatattcg tttgactgcg
     3001 tactgtttcg ataacctctt gaggaatccg ttgagccatt ttttcacccc ctgctctatt
     3061 gatatctttt gggtttaaca gaagatattt caggagaata accggtaaaa aatcgcacta
     3121 actgaaatca gtgcgatttt aaaggacaca attatacatg tttgattgta tcatatttca
     3181 ttgctttttc aagtataaca catttctatc tcattaagca tcaatttgct actcttttca
     3241 gtaactgatt ttgaatatca gccttccagt acaatactcg aagaacaata tttacgagca
     3301 tgaatacaaa gaagtatcct acgctctctc ccaaccctaa gtttacttcc aaaattccat
     3361 ggaacagcat tgcagctaca tatatcccta cgatcgtcgt tactgctagg aagatgcgca
     3421 atgggttgaa gggataacaa gcttttacaa ctgccatgca actgatccca atcagcaagt
     3481 aatacatcaa agtagtagtt tcagcatttc cccatgcgaa tgtttttcca agtagataca
     3541 ccgcaatgat attcactact acaagcaagg catttggtaa agcattaata agtgctgtag
     3601 gcaaataacg gtaagtgatt tttcttttat cttgttcaaa agataaaaag aaggaaggat
     3661 acccttcaat cgctaaatca atcagcgtga tttggatagg gataaacgga aatccaattg
     3721 ctgtaacggc acatataatc gataaaataa atgaataaat cgttttgata aaaaagatgc
     3781 ctgagacttt cgtcacattg ttgaccactc ggcgtccttc aaacaggact tctggcaatg
     3841 tagtaaaatc agagtccaac aataccagat tcgcaatctg acgtgttgct ccatcacctt
     3901 ctgccatagc aatgctgcaa tctgcttctc ttaacgccaa aacatcattc acaccgtcgc
     3961 ctgtcattgc aaccgttctg ccgctttctt tcaattcatt taccagcaat ttcttttgct
     4021 gcggactcac tcgtccaaaa accgtatagc gatgagcagc ttctcgcact tccatttctt
     4081 cagtaatttg agaaagatcg acatacgcat cataattcgg taatcctgca cgtctagcga
     4141 tattggagac agtaaaagga ttgtctccag aaatcacttt cagatccaca ccttcctcat
     4201 gaagataagc tagtgtttct tgagcgtttt ttcggattgg atcatcgatt tcaaacagag
     4261 caaccggctc aacaaatgct ggtttttctt cgttcaattg ctgatttgga gcgatcccca
     4321 acataagtac gcgatagcca ttcgcttggg ctgtttttag actgtccgga agccgatctt
     4381 cttccaccaa acgttcaggc gctcctacgt acacgactcc cagttctgga aattccatcg
     4441 cgccccattt gcgatcagaa gaaaatgcga ctttgtttgt cacatcatac cgattagaaa
     4501 gctcaaaata atctctcata gcccgcatag tgatgttgtt atcactgctt tctgccagat
     4561 acgtccccat aatttctggg atggctttct cgtacagcgg atgtagtgct tctacccgct
     4621 ggatactcat tttcccttca gtaatggtcc ctgttttatc caagcataat gtatcgacat
     4681 gggccagcgt ttcaattgaa tacatgtctt gaacaaggat tcgttttttt gccagtttgg
     4741 ttacccctgt cgttaatgca atacttatta gcaataccaa accctttgga agcatgccta
     4801 acaaagctgc tgcagatgca ataacagaaa ttttgataga agcatcgcgg atgaacagtg
     4861 cttccaaaaa tagtatgatc cccaaaggaa taatcacgaa actagtgaat ttggatactt
     4921 tgcgaatcga atctacaagt tcagaacgaa tagctttgtg agtctttgcc tcgctagtca
     4981 agcggacagc ataattatct gcacctactc ggataacttc agccatgaca gaaccgcttg
     5041 tcaaaaagct tccagaaagc aattcagctc cagtttcttt gacaatcaaa tcagattcgc
     5101 ctgtcaaaag agcttcattt gcttctgccc gtccagatat aacctgtaaa tcggctggta
     5161 tctgttctcc tgctccgatt tttaccaaat ctcctaatac cagctcttta gtagaaatcg
     5221 attgttcttt accgtcccgg atcactagta cttctgattg ggaaacaatc gataatttcc
     5281 gtacaagatt tcttgcatgt atttcttgat aaattccaat aacgatattg acgacaataa
     5341 ttgctaaata cgccatgttg gaatacgcac ctacaaataa cagacaaatc ccaataccaa
     5401 agttcagaaa attgaataac gtcaatatat tatcgctgaa tatttgtttt gtactttttg
     5461 atgcatcttc ctggtagtca ttttgctggc cagaagccat tttctgaact acctcttgct
     5521 gtgtaagtcc attcattttc tttttctcca ttttccgcca actttcaaaa ataatcacgt
     5581 gtttaagtct agcgtgttcc aagattaaaa acaacttgtt tttataaaat ttatagaaaa
     5641 acacacgaat gattttacaa aaaagatgcc aacctttttg tttgaatcac ccgtgtgctg
     5701 tttttattat caatctatct ttacttatat tgttcctcca aacggctcat ccaccaacct
     5761 agtgctgtac cggctataaa aagaacagga caagctagta ccccagcaac tcccaatccc
     5821 gcgtaatcgg taggaatgat cataatcgtt gaagcaaaaa ctacacctaa aatgaaatgg
     5881 aatagcttgc tatatgcctt tgagaaaatg taatccatga tttttgacaa tccaagaaca
     5941 caaagaatcc caccaattcc gatcggcaca atcacttcaa gtcgtacttc tttaaatccg
     6001 tcagccatcg ctttatacaa tcccatatac accaaaaaat tagaagggct caatcctggg
     6061 acgatcatac ccaaaccaat caatccgccg gcaatcagcc atgttccaaa attctgatcg
     6121 acttgcccgt caaataattg agcgccgtat aataaaaaca aataagccag aacaaaagta
     6181 acaaccatga tgatcacatc agaagtatct cttccttttt tccctgcttc tttccataac
     6241 gcaggaacag ttccagcgat acatccgaca aaaaaccata atatcgttgt ttcatacgca
     6301 cctaataaaa aactgactaa aaaagaaagt agaaagatgc ctgtcactcc gcctagacca
     6361 acaggcagaa aaaacaaaac attttctttg aaattttttg taaggtgggc taaaaaagaa
     6421 atcagccgct catacatgcc aaagacagct gctaatgcac caccgctgac tcctggcaag
     6481 ataaatcctg aaccgataaa cattcccttc acaaaccgta acatccagtc tttggttgtg
     6541 taccccactt cttgattttc cattttttgc cttttttcca ctatttgcgc actccttctt
     6601 tcatttaatt ttattcatga ttgaacattt cctgctattt tacttaaaga ctttttcata
     6661 ggtctctatt attttttcat tcattttgac aatatcagga taaaacatgt tacctccatt
     6721 atgatagaga tagccgccat tgtaaagcaa ggactggatc cgccaatatc ggtattgttt
     6781 cccttcctca tttcctaaaa gtgggctcag cacatcttta gaatacgctt ctgctaattc
     6841 tactgtgttt tctccgccat gattttttac ataggcaata taatctttcc caaaattata
     6901 tgcttggaca gctgtccata aatcgcatcc ttgtttcttt gcttctacta tcatttccgc
     6961 caaatatttg acaccttgat caatactgtc agctggatta tcaatgatct cagcttctcc
     7021 atgcagactt tccgagctct gcatgagatc tgtccctttt ccttttgatt cggtcaatat
     7081 tatcgccttg atcaaattct cgtagttttc catatgataa ttttttgcag ctgttgttat
     7141 ttctatttga tagctttcca ctcgttttac cgaacgataa gttcttcctc ctaaaaacaa
     7201 caccgcacca ataaccgtca gaatgatcat gtaaaaaaga atacggataa tagataatcg
     7261 ttttcgcttc atttcatcac ctagttattt tgacatcttt ttttatctta ctatttcttt
     7321 atcttcttgg ctatttcttc cttgatttct gtgtacgcct taagaaaaaa tgaattttcc
     7381 tgtatttttt gacatcttac gaaaatgatc gttgtttttc agtaaaaaca attgcccaat
     7441 gactgatata atcgttatac tttacacagt aggtgattat gtgaaaaaag aaaagaaata
     7501 tgcaaaaatg ccagatggga gcgaaattta ctatgaaaag agcggtcaag gcttccctct
     7561 ctttttactg catggcaatg acggcagcgg ccgctttttt tcagaacagg tccctgtact
     7621 cgaacgctac tataccgtgt atctagtaga tagccgagga catggtcgtt cgacaaacga
     7681 agcatctatg ttgaattttc agttaatggc cgaggatctg aatacgatca tgttgctgga
     7741 aaaaatcgat caggcagatt ttcttggatt tagtgatgga gcaaatctag cactggtatt
     7801 tgccagcagc ttccctaaaa aagttcatcg attgatttta aattctggaa acacgcttgt
     7861 taaaggcgtt cgtttttctg ccagagtcat cagtaatatt cattatgcat gggtatggtt
     7921 actctcttta tttcgcccaa gtcttcgcaa aaatctttta gttatcaaac ttttgctgca
     7981 tgacatcggg cttacagaaa atgatttaaa aaaaatcaac tcccccactt tgatcatcgt
     8041 gggaaaaaaa gatgtaatca agctaaaaca ttctttgtat atagctaaaa ctataccaaa
     8101 agcttccttt gtgcttgtga aagaacaagg acatgaatta gctagaaaag atcctgaacg
     8161 cttcaatcga gaagttcttc aatttctttc tgaaacataa ctattaggaa tagaaggtga
     8221 tttatttgtt aaaaaatacc atacaatggc tgaaaaacca cctaggactg ttcaaaacaa
     8281 tctttttgat ttctgtcatt gtcattattg tcggtgaatt gatgtccatc ggtaaaactc
     8341 tatctatcca gcaattagga gaaacgtttg ccactatacc tgtatggaaa agcggactaa
     8401 tgctattgat tggtcttatc tctgtgttac ctatggttgg ttatgatatt attttgaatc
     8461 ggatgcttga acaaaagcag aatcctcgtt acctgtttga gacaagctgg ctgatcaata
     8521 cgatcaacaa tattgccggt ttcggcgggt ttgttagtgt ggggcttcgc tctgaactat
     8581 atggacaaaa gaaagagagc agaaacgtga tccaagcatt atcgaaagtt ttcttgtttt
     8641 tgatggctgg actttctatt tacagtctgc tttctttttt acttgtagtc ttcacgccag
     8701 ttgctccttt tttgaagcaa tattggatat ggttgatcgg cggcggtcta tattttcctg
     8761 tcgtcttttt atttactact ttcaaaaaga aaggattcct tggtggtacc tcacttaaaa
     8821 ctcaaggaca gctattgcta gtttcattgt tggaatggac cggtgtcctg tttagtttcc
     8881 tttctgttgg ttatttaatg gaaatccgga ttgatctgtg gcaaacaatt ccattattca
     8941 ttgctgcttc agtgatagga atcgtgtcta tgattccagg agaaattggg agtttcgatg
     9001 taatgatgat tatcgggttg tccgctattg gtgtgccaag agaaatagtc gttgtgtgga
     9061 tcttgctcta tcgcctgttc tactacatca ttccgttttt gattggaatc gtctttttct
     9121 tcaaaaatat cggttccact tttgatcagc gttattctgg tatccctaaa caattagcaa
     9181 cagaaattgc gcataaaatc gtggttgtct tattatactt ttctggaatc atgctagtac
     9241 tttctgccac tatcccgcaa gcttttacag agtttcgctg gcttcatagt ttgaacccat
     9301 tgaaatttca ttttattatc caatttccta gtatactatt gggatttctt ttaatcatta
     9361 tgggcagagg gatcgcagca cgtgtgaaac gagcatattt gcctacaatc ttcttgattg
     9421 ttctagcttt actttatgtt ttattaagtg attttagttt cacaccggtg gttttcttgt
     9481 ctattctgct tctgatcatt cttgcttcta aaaacgaatt atttcgggaa caattgatct
     9541 attcatggga gtggcgtacg atcgatggaa tcattattgg tgcactgtcc ttgctttaca
     9601 tcgttattgg ggtatacaat ctgccagatt tccctcatcg gcgtcaccat tttatttcct
     9661 ttttcttatt tccttctgaa aagatttggt tttcgggatt gttggccatc atcgctgtta
     9721 gtttcatgat cgttctattt gttcatttcc ttcaaggaga aaagaaacag attggcgaag
     9781 cctttaatga agaaaaggcc ctgaagatcc ttacaactta tggaggaaac tccgatagtc
     9841 aattgatctt cttaaaagat aagcggatgt ttgcctatga aaaagacggt gaaccaactg
     9901 ttcttctcca attcgcttgc ttcaataata aatgtattgt aatgggtgat ccttctggta
     9961 agaaagaaga ttttccagaa gcaatcgaag cttttatcga agaaacagat cgactatgct
    10021 atttaccagt tttctacgaa acaagcgaag agattgttat gattttgcat gaattcggct
    10081 acgatttcat taagatgggg gaagaagctt atgtagacct gaatagtttt acaacttcag
    10141 ggaaaaaaat gaagggcact cgtgctgtct tgaatcggat cgaacgagaa ggatttacat
    10201 ttgatgtctt acaaccacca ttttctactg agcaaatgag tactttcaaa aatatctctg
    10261 ataattggtt gggttcacga aaggaaaaag ggttttcttt aggctttttc tctgaagatt
    10321 atctacaaag agcgcctatt gcagttgtca aaaataccca agaagaaatt gtcgcttttg
    10381 caacgatcat gcctacttac acgaacaata aagtaggaac gattgatctg atgcgatatg
    10441 attctaaaaa agcaccttct ggcagtatgg atttcttgtt cttgcatttg tttgcctata
    10501 tgaaagaaga acatatccaa tggttcaatt tagggatggc ccctctttca aatgtcggaa
    10561 cttccagaaa gagtttttta caggaaagga ttgcatactt ggtttacgag tttggttctc
    10621 atttttattc tttccatgga ttaagagaat ataaaagcaa gtacgctaca aactgggtct
    10681 cacgttatac tctatattct agagatagtt ggattgcata tgttatgatt gctttgctga
    10741 ttattgataa tgcgccagtt gagcagacac aaggtagaat aagtggtttg aaaaaatgga
    10801 tacgaagaaa gtattaaaaa ctgcataccg atagatgaac actctcatga tgaataaaag
    10861 agactgaagt aaaacgaaag ttcatgcttc tacaatctct tcttatacaa ttgaagaaca
    10921 aagagcggtc caaacagaaa tcctgtttgg accgctcttt tattttaaac atttttcatt
    10981 accaaaatat tcgagtcgcg tttcttatgc ttcgatctct gtaacgatac ctgaacctac
    11041 tgtccgtccg ccttcacgaa ttgagaatgt cgttccgttt tcaacagcga ttggatggat
    11101 caagtcaaca tcaatcgtca cgttgtcacc aggcatgacc atttctgtat cttctggcaa
    11161 tgtgattgtt cctgtaacat cagttgtgcg gaagtagaac tgtggacgat agttgttgaa
    11221 gaatggagta tgacgtccac cttcttcttt tgtcaacacg tacacttctg ctttgaattt
    11281 tgtatgtgga gtaatggaac ctggtttagc aagtacttgt ccacgttcga tatcgtcacg
    11341 ttggatcccc cgtaacaaca cgcctacgtt atccccagct tccccgtaat ctaacgtttt
    11401 acggaacatt tctacacctg ttacaactgc tttttgtgtt tcaggtttga tccctacgat
    11461 ctctacctca tcaccgacac gaacagcacc acgatcaata cgacctgagg caacagttcc
    11521 tcgacctgta attgagaaga cgtcttccac tggcaatagt aatggtttat ctgtatcacg
    11581 ttctggtgtt gggatatatt catctactgt atccatcaat tccataatag cagcttcagc
    11641 atctggatcg ccttgcaatg cttttaatgc ggaacctttg atcacaggag tgtcatcgcc
    11701 tgggaaacca tattcgctca ataactcgcg aacttccatt tctaccaaat cgatcaattc
    11761 ttcatcatcg actaaatcaa ctttgttcaa gaaaacaatc aggtatttta caccaacttg
    11821 gcgtgacaat aaaatgtgtt cacgtgtttg aggcatcgga ccgtcagttg cagaaacaac
    11881 taagattgca ccatccattt gtgctgcccc tgtaatcatg tttttcacat agtccgcgtg
    11941 tcctggtgca tcaatatgcg cgtagtggcg tttttctgtt tcatattcta cgtgtgcggt
    12001 attgatcgta atcccacgtt cacgttcttc tggagctgca tcaatactag catagtcttg
    12061 aggatttgct aggccttttt tgcctaatac tgtagtgatc gcagcagtta aagtagtttt
    12121 cccatggtca acgtgtccaa tcgtaccaat gttaacgtgt ggtttacttc tgtcataatg
    12181 ttcttttgcc ataatttaca aacctcctaa ataatataga tcactgaaaa atttcagctc
    12241 tcaccttttt attatagtct ttttttgact aaatcaaaaa gaatacgtcc gacttacttt
    12301 ttgtaagatt cgaactaagc ttattatacg tcaatattgg tcaaagtcaa ctaagagcac
    12361 tcgtctgtct tatacgcgaa aaaagtacct tttataaata aaaaaatgtg ttttttgaga
    12421 aaaacttcaa gatagtcctt aactttcttt tgaagcaata agaacaaaat gaggaaaagt
    12481 ttaaaattca gctgttgcca acaactttca atagacgaaa tcttctattt cgttaataat
    12541 aaggaaagag gtgaagtaca tgaaaaaatt aattggcgta cttgtcgttt taggcatatt
    12601 tctcggcgga agttttgcag cttatcatta tttttacggc ggcgaagctt attatacaaa
    12661 aatcacgacg aatggcgaaa tgtcagctac ccaagcagat aacggagaga aattcacgac
    12721 ttatacctac aagcaagcgg cttataataa agatggtgaa aaaaaacagg tgacgcttcg
    12781 cgaagaaaga gaacatccat taaagatgaa tgcgtatctt aaactcaaag tcaatcctcg
    12841 taaaggtgta ttaagctggg aagaaataaa ggcttctgaa gtgcctaaaa atgcggcaga
    12901 aaaaatcgat aattagtaaa tggtcttaaa attttattag ataaaaagag gctgggacag
    12961 aagcgtttaa ctccaagaaa taagaaggag ttgcttctgc ttccgccgtt tatacgtgtt
    13021 tagagcgtga gacaaaagtg ttctttactt ttgtctcacg ctcttttt
//
LOCUS       DACDRN010000065        12716 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDRN010000065 DACDRN010000000
VERSION     DACDRN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.065"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..537
                     /locus_tag="IWJ80_002170"
     CDS             <1..537
                     /locus_tag="IWJ80_002170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461873.1"
                     /translation="VMAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNG
                     QPMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLD
                     MTIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQA
                     IIDSGRVTQWKKIRPFIF"
     gene            564..2375
                     /locus_tag="IWJ80_002171"
     CDS             564..2375
                     /locus_tag="IWJ80_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9461874.1"
                     /translation="MNENGEFEESKKYQATSEEEKAAVRELLEEAVPTDFLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVREIEATPEDKKR
                     IESGQIEVIKKTYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQ
                     AVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIH
                     ELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTLEKAIPYIAAWTKNGQTLDDK
                     EAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAEQKNI
                     VPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSE
                     EFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2436..3905
                     /locus_tag="IWJ80_002172"
     CDS             2436..3905
                     /locus_tag="IWJ80_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAP9461875.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3916..5925
                     /gene="topB"
                     /locus_tag="IWJ80_002173"
     CDS             3916..5925
                     /gene="topB"
                     /locus_tag="IWJ80_002173"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9461876.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            6271..6867
                     /locus_tag="IWJ80_002174"
     CDS             6271..6867
                     /locus_tag="IWJ80_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9461877.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            6880..7779
                     /locus_tag="IWJ80_002175"
     CDS             6880..7779
                     /locus_tag="IWJ80_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9461878.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            7782..7913
                     /gene="ssb"
                     /locus_tag="IWJ80_002176"
     CDS             7782..7913
                     /gene="ssb"
                     /locus_tag="IWJ80_002176"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9461879.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            7935..8267
                     /gene="ssb"
                     /locus_tag="IWJ80_002177"
     CDS             7935..8267
                     /gene="ssb"
                     /locus_tag="IWJ80_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9461880.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            8289..8546
                     /locus_tag="IWJ80_002178"
     CDS             8289..8546
                     /locus_tag="IWJ80_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461881.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(8705..8827)
                     /locus_tag="IWJ80_002179"
     CDS             complement(8705..8827)
                     /locus_tag="IWJ80_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP9461882.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            9017..9241
                     /locus_tag="IWJ80_002180"
     CDS             9017..9241
                     /locus_tag="IWJ80_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461883.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRNQLVIESMQIRNPDHFTRLMGI"
     gene            9689..9895
                     /locus_tag="IWJ80_002181"
     CDS             9689..9895
                     /locus_tag="IWJ80_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9461884.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPYTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9895..10146
                     /locus_tag="IWJ80_002182"
     CDS             9895..10146
                     /locus_tag="IWJ80_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461885.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            10162..10599
                     /locus_tag="IWJ80_002183"
     CDS             10162..10599
                     /locus_tag="IWJ80_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461886.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGQWFFQALLCLEAGVKPNTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYLENVNKYGACYIMKKEDNENE"
     gene            10592..11287
                     /locus_tag="IWJ80_002184"
     CDS             10592..11287
                     /locus_tag="IWJ80_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461887.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEELNSKDATVDDYYLVFTKFLTCGTE
                     SIVLMGMDYELAVPEVFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRK
                     RMPYQRFERKKGQ"
     gene            11668..11847
                     /locus_tag="IWJ80_002185"
                     /pseudo
     CDS             11668..11847
                     /locus_tag="IWJ80_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11909..12093
                     /locus_tag="IWJ80_002186"
                     /pseudo
     CDS             11909..12093
                     /locus_tag="IWJ80_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, HicA family"
     gene            12127..12558
                     /locus_tag="IWJ80_002187"
     CDS             12127..12558
                     /locus_tag="IWJ80_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAP9461888.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT         4683 a         2131 c         2683 g         3219 t
ORIGIN      
        1 gtcatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccagaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacaa
      241 gtagaaaaac ttgaaagaca agaagagata aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgagaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc ggaaaaagga aaagaaaacc ttcttcctga agtacaagca
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaatcc gaccatttat tttctgaaag
      541 gctatcgaaa agtcgcttta gaactgaatg aaaacgggga atttgaggaa tctaaaaaat
      601 accaagccac gtcggaagag gaaaaagcag ctgttcgtga attacttgaa gaggctgtac
      661 caactgattt tttgaaggag aaagaaaacg atcattttta tcgagtagaa ttcaacgaaa
      721 atgatccaga aaaaggattg aaaagttatg gtggggagat cgtaacattc gaattgatcg
      781 atcaactcaa aacgttggat aaccaatacg ccgcagaagc aggttattat aaattctatt
      841 ttgaagaagt gaagaacgga gaagtagtcg atcagatccg aatggatcta ggggatggcg
      901 tcaacagtaa tcaacccatc tatttagagt tagcaaaatt gtgcgaacaa gtacctgaga
      961 aggaacaaaa acaatcggtc aaaaatgaac ctgtttcgaa agaacaaatg cagtcgaaag
     1021 aaacaaaaac aaaacaaaag ggctatcaaa aacgaacggc agaagagatt aaacaagaag
     1081 ttaaatccct ttcgcaacag gcactggatg cggtgaaaaa gcatactcat tctcctgaag
     1141 acgtcaaaga acttttgaac tttatgtctc gttttccaga acgatccttt agaaatcaat
     1201 tattgattga ggaacaattt ccaggtgcaa cggcttgttt aggtcgtgcc gcgttgaaaa
     1261 aagaaggaat ctatatcaaa aaaggggaac aagggaacaa gatctttgtc agaaaaacgg
     1321 tcaaaggatt ttatgagaaa ggacgtggct ttgttcgaga aatagaagct acaccagaag
     1381 ataaaaaacg aatcgaaagt ggtcaaatcg aagtgatcaa aaaaacttac tacacgattg
     1441 aaaaagtgtt tgatatcaca caaacacagt taaaacctga agactatccg aaaatctttc
     1501 cgaaccgtgt ctttgatttt caattggata aaaaaggaca gtcagaactt caagctggga
     1561 ttcaagcggt agccaacaat attggaatac aaattcgaga tatgacagaa agtgaagtct
     1621 atcaaagaga attaggacaa gcacgtgggg cgtatgttcg taacgaattt actggcgaag
     1681 aagaaatcgt catgaatact cgaaatactc ctacgcagca tttagctaca agtattcatg
     1741 aattagccca tgcaagaatg cacaaatttt cagaattaga taccgcaact aaagaattgc
     1801 aggcagaaat gacttcttat attgtgtgca aacattttgg aatggacacg ttggagaaag
     1861 ctattcctta tattgcagca tggacgaaga atggtcagac gttggatgat aaggaagctg
     1921 cggaacgtgg gaaaatcatg aatgatgtca gccgtgtcgc aaatgaattc attcaaacca
     1981 tttctaatga aatcaatcaa tacagagaaa gagaaccaga gatccaacca gcagaagtga
     2041 agcaggagca taaaaatact gtagaaacgg cagaacaaaa gaatattgtt cctgttggtt
     2101 cactttggat cgacaagaaa gacgggaaag taatggaaga agatacgggt cgaacgttac
     2161 atttgaagga tgcggcgttt attgaagatc cacagcataa tcaagtaatt ttaaaaggaa
     2221 aaagttatgg aaaagagatc caaacggcgt taccttcaga agaatttgcg ctaggaaatt
     2281 cattccgatt ttcacttgag acagtgtcag aagtggcaaa acgaaaaaat gaaatctatt
     2341 atgaacaaca attgtcacaa aaagaacgtg tataaaacaa gtaaaaaaga ctatctgaaa
     2401 aacggatagt cttttttctt ttgggaggtt ttaaaatgac cgagaaaaaa atgagtctta
     2461 ttgatcgatg taaacagatc gatatagtgg attttgctcg aaacaatgga atggctgtag
     2521 tcaataaagg acgagactat cgcttagaag accatgattc atttgttttt gatcgaagaa
     2581 aacaacgttt ttattggaac agtcaaaata ttagtggcga cattattgaa ttagctaaac
     2641 tttttttcat agacaaagag atccaagacc ctaagcaaca atttaaagca gccactgatt
     2701 ttattctaaa gaatgaagac aaaacagaac gagtggaaaa tcttcatttt gagacagaaa
     2761 agtacaaaga tcaccccgtt gattatcaac cactcacaga aaaaggaagg aattatctga
     2821 aggaagaacg aaaactacca gagtggctga ttgattatgc agaaaaagaa gggttgattg
     2881 ctgaactaaa gccgaaacat gaacggcaaa attttttagt tggagacgac cgtttagatc
     2941 atgcggttgc ttttttatgg aaagatccac agacaggaga gacagtaggg gcaagttatc
     3001 aaggaacgat cgtcgatttt aatcgtttcg gtaaacgggg aacgtataaa cacattgaca
     3061 agaatcctac gccaaatcac ggatttaatt taaaaatagg cgatccgaag catttaaaat
     3121 tctttgaaag tagtattgat ctgttaagtt acgctgcatt gaatcgggag aaactacaag
     3181 atgcctggtt agtttcgatg gacgggttaa aacatcatgt aatcagtcat tacgttgaag
     3241 agtcgatttc tgaattaaga cgaaaacaaa catttcctca atcgattgaa atatgtgtgg
     3301 acaatgatcg tgcaggacat attttttatg aaaaagaaca gatgaaggga atcgttgatc
     3361 cttttaccaa taagaaaatc cgttgtgagc gaggaattcc aaatgattgg caagtaccta
     3421 aagaatacaa agctacttat gaagcagtcg caaaagaaat gagtgttgaa cctgaagcaa
     3481 ttatggccat acacaaaact gaaactaatt tacagctgac aaatcaattg gtgtctgctc
     3541 atgatgttca atctacgttt ggaaagatgt tggcgaaggg cgaaccagta gaaacaattg
     3601 atttgaaaga agcatgtacc acggtcgcga aggaactgaa agtatgtgaa cgcgcagatg
     3661 gaacatacaa ctttgatcgt ttttatagta gaaaagccaa cataaaagac gttaatgcag
     3721 gaatccttct ttcctataaa gcagaacaat actacaaagg ctataaaaag catgaacatg
     3781 aatttgtacc tgaagtgaaa aaggattgga atgatcaatt gaagcatgag atccagcaac
     3841 aagaaatcag aaagcaaaaa cgtgcgatgt tgtttcaaca aggcagacaa caagaaaggg
     3901 agtgaagtcg tcactatgaa aaccgtcatt ttagcagaaa aaccgtcaca agcgaaggct
     3961 tatgccgata gcttttctaa agctacccga aaagatgggt attttgagat ccaagatcga
     4021 ttgtttacag gagaaactgt gatcacgtat ggttttggtc atttagtcga gttagattcg
     4081 cccgatatgt acgatgaaaa atggaaacaa tgggcattag aacatttgcc tattttcccc
     4141 aatcaatacc actatcatgt tcctaaagat aagaaaaagc agtttagtgt cgtcaaacga
     4201 cagctgcagt cagctgatac gattgtgatc gcgactgata gcgatcgtga aggggagctg
     4261 attgcctggt caatcattca acaagcaggc gccaatcaag gaaaaacctt taaaagactt
     4321 tggatcaact ccttagaaaa agaagccatt tatcagggat tccaacagtt acgagacgca
     4381 gaagagacct atccaaaatt tgaagaagta caagcacgtc agatcgccga ttggttaatt
     4441 gggatgaacg gtagcccact gtatagttta ctcttgcagc aaaaaggcat accaggaagt
     4501 ttttctttag gtagagtcca aaccccaaca ctctatatga tttatcaatt gcaggaaaag
     4561 attagaaact tccaaaaaga accatatttt gaaggaaaag cccaagtaat cgctcaaaat
     4621 ggcgcatttg atgcgaagct tgatccaaat gaaacccaag cgacacaaga agcatttgaa
     4681 gcctatttaa aagaaaaagg tgttcaatta ggaaaacagc caggtacgat ccttcaagtc
     4741 gaaacagaga aaaaaagtgc ggctagccca cgtctttttt ctttatcgag ccttcagtct
     4801 aaaatgaatc aactcatgaa agctagtgcc aaagatactt tggaagccat gcaagggttg
     4861 tatgaaggaa aatatctaag ctatccgcga acggataccc cttatattac ggaaggggaa
     4921 tatgcgtatc tgttggatca cttagacgaa tacaagcatt ttttaaaagc cgaagcgatt
     4981 ccgactccta ttcatacacc gaatagtcgg tatgtgaata ataagaaagt ccaggaacat
     5041 tacgcgatca ttcctacaaa aacggttatg acagcagcgg cttttgaaca actaagtcca
     5101 ctgcagcagg ctatttatga acaagtgtta aaaacaacgg tggccatgtt tgcggaaaag
     5161 tatacctatg aagaaacaac gatcttgacg caagttcagc agcttcagtt aaaggcaatc
     5221 gaaaaggtgc cgctagatct aggttggaag aagttatttg gaaaagagag cgacggaaaa
     5281 gagaaggaag aagagccgct acttcctaaa gtgacaaaag gagaaatggt gacggtggat
     5341 ctacaagttc ttgaaaaaga aaccaagcca ccacaacctt ataccgaagg tacgttgatc
     5401 actgcaatga aaacagctgg gaaaaccgtg gatagtgaag aagcgcaatc catattgaaa
     5461 gaagtggaag gaatcggtac agaagcgaca cgggcgaata ttattgaaac cttgaaacaa
     5521 aaggaatata tcaaggtaga gaaaaataag ctcgtggtga cgaataaagg gattttgctt
     5581 tgccaagccg tggaaaaaga gccgctgctt acgagcgccg aaatgaccgc caaatgggaa
     5641 agctacttgt taaagatcgg tgaacgaaaa ggaacacaaa ccacttttct agccaatatc
     5701 caaaaatttg tctctcactt attggaagtc gtaccagggc aaatccaatc cactgatttt
     5761 ggatcgacgt tacaagaagt gaaagcagcc agtgaaaagc aagaagccgc gcgtcattta
     5821 ggaatttgtc caaaatgcca ggaacaagaa gtcttactct atcaaaaggt ggcagcttgt
     5881 acgtctgaag cctgtgattt taaaaggtca gaagggttca tttgaagcaa cgattgtttt
     5941 aaaagaagac ttcactacag gctttgaatt ttctgaaaag aaaaaaacga acttcaagaa
     6001 aagaacacgc cgcactgcaa aataatggag ggttaatctt atgcgtaata ccacacacaa
     6061 acaacaagaa atcgaaaatc aaaaagccaa aattcgttca gaacaggaaa aaatgcgtgc
     6121 cagcaaacga agactgcagc aattgaagaa ccagcagagg attcaaaaag aaatcgaatt
     6181 agatcgataa aatcttccta actattgtat acgtataaac ttatgttata ctgtttttat
     6241 aagtttatac gtatacaaag gagggagaaa atgacaaaag caatggtata caagctgcta
     6301 gaagaattta atggaacttt ggctttgaag gatgccaaaa aagcgggcat ttctcctgta
     6361 acgatcaaac gtatggtaga tcgaggggag ctggatcgag agtatccagg ttttttcacc
     6421 ttacctggtc aatttcctga tgaattgttt atggcgcaaa aaaaatatga acgcggcatt
     6481 atttcacata ttaccgcgtt agatttgtat gatttaacag atatgatccc tagacagatc
     6541 gatctgactg taccttacgg ttatcacgtt tctgaaaaag gattgaagga attcgcagtt
     6601 gagctacact atacaaaagc ggaatggtat gaattaggta agattgaaat aaaaagtcgc
     6661 tatgggaatc ctattatagc ttatgatcca gaaagaactt tatgtgacat ttggaatccc
     6721 tggtataccg ttgaggatga aatcaaagta aaagcaatca aaaactatat ggagtcggac
     6781 agaaaaaatc ttagaaaatt gaatgagtat cgacgaattt taccaacaga caaaacgatg
     6841 cgttcttata ttatggcttt aaattaaagg agaagataca tgacacctac gcagttaaaa
     6901 gcaaaagtga agaatatttc tagagaaaaa gaagttgatc cgcagcttgt tttaagacat
     6961 tttatgatgg aaaaattctt ggaaaaaatc tcagagtctc cttatcgtga aaattttgtg
     7021 ttaaaaggcg gcttcttgat tggctcaaag tatggcattg aaaatcgcac aaccaaagat
     7081 attgatacga ctttacggga aatgaaagta accaaagaaa cattaacaac ggttctaaac
     7141 gaaatttttt caacgccgac gaaagaaggc attcaatttg agattcaagg aatgaaagaa
     7201 acgagagaag ccgattacta tccaggattt agcttacgtg tgctggcaca tttggaaaac
     7261 atgcggccag attttaaggt agatgtgact acaggtgact cgatctatcc tgcaacgatc
     7321 actcacagtc ataaattgat gtttgaggat cgaacgattc ctttggaatc ctatcctaca
     7381 gaacaaatta ttgctgaaaa attaagtgca acctttgatt ttttaacaga taattcgaga
     7441 ggaaaagatt tttatgatct ctatactatt cctaaaatgg agaagatcga tgagaagacc
     7501 ttgaaagata gtgtgagaaa tacatttaca cgtcgaggaa aaactgatcc gttaaagata
     7561 tactatgaaa aggatatgga tgtactgaaa aatagtcacg agctaaaaga tacctggaag
     7621 aaatatcaaa aaagcaatcc ttatgctgcg tcgatcacgt acgatgagac aatggattcg
     7681 attagtaatt tgatggaaaa agtgattcac gtcgaagaaa aagagcgaaa actagcttat
     7741 caaagacacc aacagatgaa tagagaaatg gagagataaa gatgattaat aatgtgacat
     7801 tagtaggacg cttaaccaaa gatcctgatt tacgttatac cgcaagtggt actgcagtag
     7861 cgacttttac cctagccgtc aaccgaaatt tcacgaatca aaacggcaat tgagaagccg
     7921 attttattaa ctgcgtgatt tggcgcaaac cagcagaaac aatggcgaca ttagctaaaa
     7981 aaggtagctt gattggtgta gttgggcgga ttcaaacgcg gacttacgac aaccagcaag
     8041 gacaacgtgt atatgtgact gaagttgtag cagataactt tcaactatta gaaagtaaag
     8101 cagcgacaga gagccgtgcg cacgctgatc aatcgtccac cagtccaagc accgccacct
     8161 ttgaacaacg gaatacagcg acgcctaaca acaatggatt gaacgcttca cagaatccat
     8221 ttggtggaca atcaatcgat attagcgatg atgatctacc gttttagttt gagaaagaag
     8281 gagtaagcat ggcaaaaaaa attagtctga cgatggatct attgataccc gtaattgcat
     8341 ttatagacgg gatttctttg ggcgtcactg aaaatccttt ggtaaataga attgcgaaag
     8401 gatcactatt tcttgtattc gtgtatcttg tctgttctta tcttttaaaa agagcaggaa
     8461 aggataattc cttttggcga ttgactaaag tcacagtagc agcgacagct gttatactta
     8521 cggctattct accgctttat atgtagagtt aacgggaaat cgttgaactt cttgcgaaat
     8581 attgctataa tgaacatgaa aaagaggagt tgcgctaaca acccctcatt aacagcaccg
     8641 attaaagaac ggtggcagaa tattagttat ttggaaataa ccgctgacct gctaaagtag
     8701 acggttattt ctttttgtca tttttaagca tatttacaat caaactaatc aaagcaatgg
     8761 taaacatacc aaaaccaaga atggtttgaa ttgtctcata tgcggacaaa aggcttactc
     8821 ctttcataga ttttagcact tacattcata agcaccacca cctttccaga agtcgccacc
     8881 gtcttcaaac tttactgtct tgactattat agcatatcta aaatcgaata aaaattctca
     8941 attttagctg tttttgctat aattttagcg aggtgatcgt gatggaacaa tatattggtt
     9001 tactcgataa attagggtga tcaagacaca gccattgctt gtgcgattta cattacgcac
     9061 gagtcatgaa ccaatcaatt gtgtagtggc caatatagaa atagcggata aattgctgat
     9121 tttggaagaa ggcaagtata ctatttcagt gacaggtcac ttcaataaac gaaatcaact
     9181 agtcattgaa tcaatgcaaa ttagaaaccc tgatcatttt acgagattaa tgggcatata
     9241 aataaaagaa gagctgcagt cctaaatgag gattgcagct ctttttgttt tacatagaaa
     9301 caagaaaaat gaaaaggcct ttttaggaag gcgcaagcgc cttctcaatt gaaaaacctc
     9361 tctctcactc caaccgccac cgccgttcga gaagaaagga agtgaacgtt gcacgttcac
     9421 taagataagg ggtcttgcaa agcaagacaa aatagaaaaa acttcctctc accaaaaggt
     9481 tcgttcctgt tcgctatctc tttattgatt tgccagaccg gcaacaagtt gccttttatc
     9541 accaaaaagc gacaaggaca agggtaaatg gcacgctgca cgcccttgtc cttgtcgctt
     9601 ttagttgatt cattctggcc aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa
     9661 cctttcctaa gaggaggcag aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc
     9721 cttcagcgaa ttattcccaa actatcatgc accatatact gtcgaatgcg attgtggaga
     9781 aagagcgaaa tgtatcaaaa gctattgttt gtatcaatgt ccaacatgcg ggaaaaaata
     9841 taccttgtct tatggcgatt atattttgat tgatgagaaa ggaaagaaac gctgatgaaa
     9901 acacaacaca aattattgac tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc
     9961 atttctgaaa aagtagcaga agctttactt attccgcggt actggacgat agaaacccct
    10021 ttagtagagg atgcaccgac tatgctattt gaagacctag tgaagattgc aggatatgaa
    10081 ctgcattatc aagaaaacat ggaaacttat gaaaacgaat attggttaac caaattagag
    10141 aactaaaaag gagaaaaaaa tatggctcaa aattactatg acgaatttgt caaattacct
    10201 ttggataaaa tggcacaaaa aatggaggat atgacattcc tatacaatga gacacgtgtt
    10261 ccaaaaaaac attataagga aagattgtcg gtagctgtgg aagaaatgat tgaatcgggt
    10321 gtcgaaatga atctcattgc gacctactat cgaacgcttg aagaactgaa aaagcaaaat
    10381 ggacaatggt ttttccaagc cttactatgc ttggaagcag gcgtcaagcc aaacactatc
    10441 aaaccttctg aataccaagc tttagaacta acgtatggga aatttgtcga aacgaaaaaa
    10501 gccaagactg tttcaagtga atggttggac tacttagaaa atgtcaacaa gtatggcgca
    10561 tgttacatca tgaaaaagga ggacaacgaa aatgaataag gtgatttatc aactaacaac
    10621 ggaagatgtg tcacactttt taacaaacaa agaacatttg aatgaagtaa tcagacggat
    10681 tcaaaaagac tttagttgta tggaagaaat gaagtacttt attcaaagag tcgaagaaca
    10741 attgtatttg gaaagtgaaa cgagtcaaac gaaggttaca gtcagcaata attatttaga
    10801 cgtgtataaa cgtcgtcctg acacattaaa aaaaggagcg gagcttctac gactagaaac
    10861 agaaaaatat atcatgaatg tttacctaga aaggagtgaa ctggtcgaaa actttgtcag
    10921 ctacacgatt tgcaggaaac actattactc agaatcagaa tattattcaa tttttgagag
    10981 catggaatta atggaagagc taaacagcaa agatgcgacg gtagacgatt attaccttgt
    11041 atttactaaa tttttgacgt gtgggacgga atctatcgtg ttaatgggaa tggattatga
    11101 actcgctgta cccgaagtat ttttgaggga atatgtagaa gaagtagata acccaggcga
    11161 cgactggtac acgactttaa ctgatttttt agatacctac acttacgacg atacagaaga
    11221 aatccaaaat gaacttgacc gtaaacgaat gccatatcaa cgatttgaaa ggaaaaaagg
    11281 tcaataacca tttataccga aaagtgagaa tgtgtgaaat aaaaacaaaa aaccttgaaa
    11341 ttaagggaat ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt
    11401 gcgaaataag cgagcaaagc ccgaaaacct aagagaaaga aggagattta tatgaacaaa
    11461 attattccct tattattagt cctatttgtt ggagaaatac ttatgcgact tagtgcagct
    11521 gtacatttaa acctcgcagt atttttggta atactaatgt tttgtttgat cttttggcga
    11581 tttgtattag ccctcgtaag tctactattc aagatgattg cagccatatt tttcatagga
    11641 ttatttcgat gaaaggagag agtaccaatg ccaattgcaa taggaaataa acggttaccc
    11701 gtgacgttgg atgaaaagag acaaaaagaa ttgcagtaac caaagcagaa gtacggcaaa
    11761 agtgaatcca ggattatgtg tattgcgtta gatttattga ttgcgcaaga aaaagcagga
    11821 tttgacatac cagcactcaa aaagtgatcg cactttgcgc acgtgttaat acgtgttata
    11881 attattttgt aaggaggtat agtgcacaat gccacttacg ggaaaagaaa tgcttaagtt
    11941 attgaaaaaa atggctgggt tgagcgaagg caagagggat ttcatcatca tctttataag
    12001 gatggtgtta gaatcactgt tcctgttcat gccaatcaag atcttggccg aggacttgag
    12061 cgaaaaattt taaaggatgc gggattgaaa taatctcgca ttcgattaaa gaaaggattg
    12121 actgaaatgt taagtgagcc tttagcaaaa acctatcctg ctatttttag cccagaagaa
    12181 ggtggtgggt actttattga atttcctgat gttcaaggtg cttacacagg aattaacgaa
    12241 gacgatatat cttatggaat tgctatggca caagaggtgt taggtatggt tttagcagat
    12301 tatattgagc atgaagatct acttccagaa ccaacaccaa tcaataagat ttctgttgaa
    12361 gatgattctt ttactacttt aattcgtgta gatgttgcca aatatttgaa agacacagaa
    12421 cttgtcaaaa aaaccttaac aattcctcaa tgggcggata agctaggcaa aagggctggc
    12481 ataaattttt cagtccttct aacagaatca attgcagata aagcagataa cattcttcgt
    12541 tctggaagaa ataattagaa ctcaaaaggg tcagaaactt aaaaatcttt ggcttctttt
    12601 tttactacaa agatggcttc tttaaattat ttgcctgatt gatttaaaaa aattgcgcaa
    12661 agactacgag ctaattaagt tgctcgtggt ctttttttat gaaaactttt tagatc
//
LOCUS       DACDRN010000066        12690 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDRN010000066 DACDRN010000000
VERSION     DACDRN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.066"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            200..2083
                     /locus_tag="IWJ80_002188"
     CDS             200..2083
                     /locus_tag="IWJ80_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9461889.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2158..2442)
                     /locus_tag="IWJ80_002189"
     CDS             complement(2158..2442)
                     /locus_tag="IWJ80_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9461890.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2432..3166)
                     /locus_tag="IWJ80_002190"
     CDS             complement(2432..3166)
                     /locus_tag="IWJ80_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP9461891.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3295..3918
                     /locus_tag="IWJ80_002191"
     CDS             3295..3918
                     /locus_tag="IWJ80_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP9461892.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            4110..5231
                     /locus_tag="IWJ80_002192"
     CDS             4110..5231
                     /locus_tag="IWJ80_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP9461893.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5238..8366
                     /locus_tag="IWJ80_002193"
     CDS             5238..8366
                     /locus_tag="IWJ80_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP9461894.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8385..11579
                     /locus_tag="IWJ80_002194"
     CDS             8385..11579
                     /locus_tag="IWJ80_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9461895.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11725..12432)
                     /locus_tag="IWJ80_002195"
     CDS             complement(11725..12432)
                     /locus_tag="IWJ80_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9461896.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4329 a         2227 c         2668 g         3466 t
ORIGIN      
        1 ctaccatagt agaatcagca aagaaaaact gcttttttat ttgaaatttt cttaaagttg
       61 aaaaaaagca tgtttatttt tttaaattta tgggatgata gaaaaaaata gtagagaaag
      121 gacacgattc tacttgctat tttcacttaa tacccgtata ttacgaatga aaaaagttta
      181 tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc gctacatcac
      241 tgggtttgat gggctccgtt cattagctgt ggtgggtgtt atcttctatc accttctgcc
      301 aactagtatg aaaggcggct atttgggcgt tccgattttt ttcgtcgtat ctggttattt
      361 gatcacggat ctgctgcggc aggaatggga acaaaatgga aaaatcaata tttggcaatt
      421 ttatgtcaga agaatgaaga gactctatcc ggcacttgtt tttatgcttg tgacagcttc
      481 ggcttatatc actttgtttc agcgagggct gttaaacaat cttcgaggag ttgtggtcag
      541 cagcttactg tatgtcaaca actggtggca aatcaataac ggtttatctt atttcgatcg
      601 atttgctaat gagtcaccgt ttacgcatat ctggtcttta gcagtagaag gtcaaaacta
      661 tttggtttgg ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa gaaaatggat
      721 cttttatact gtgttaggta catccttcct ctccgccatc ttgatgatga tcttgtatac
      781 accaggaggc gatccaaccc gtgtttatta cggtaccgac actcgtctgt tttctatatg
      841 gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa acatcccgaa
      901 acaagcgaaa aatattctga atatcgctgg aggaatctcg cttttggcac tgattctttg
      961 cttcttcttt ttagatgatc attacaattt catctattac ggaggaatgc tcctagtgag
     1021 tctgttatgc gtagtgctag ttgcagtgac agcacacccc ggagcaagtt tgaatcgatg
     1081 gctgacaaat ccagtattta cttggattgg caaacgaagc tatggcattt atctgtatca
     1141 gtttccagtt atgatttttt atgaagcaaa ggtgactgat attgccgatc atgttttgtt
     1201 gcatacttta gtggaaatca cattgattct aatcatcagt gaactttctt atcgatatgt
     1261 ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc caagtgatga aaggatggtt
     1321 cagcaaaccg atcatcagta agagcaaacc atgggttgtt ccaggcacat tacttgtagc
     1381 aattgcactc gttggtttcg ttacggcacc gaaaaacgct gtaacagccg atcaaaaaca
     1441 actgcaggca aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa acaattcaga
     1501 aaacaccact agttcggttg accaagcgat ccttgataag tacgagttgt ccaaggcaca
     1561 agggaataag gcacaaaaat tagaactaac ggcatttggt gattcagtta tgttggatgc
     1621 tgcaacggat ctacaagaac tttatccaaa agtagtggta gatggagatg tcggacgaca
     1681 attatatgcc agtgtaccgt acattgaaaa attaaaggaa caagatttgt tgaaagacac
     1741 tgttctgatc ggactaggaa cgaatggctc attctcggaa gctcagtttg atagtgtgat
     1801 ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga cgaagcgctg
     1861 gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca atatgacgct
     1921 gattgattgg tacagctaca gtaatgacca tagtgactgg ttttatgatg atcaagtcca
     1981 tccaaatcct gaagggatga cacattacat tcacttagtc agcgaagcct tgctaggagg
     2041 aagttcaagt acgaacagca gtgagacaaa cgtgacagaa tgatagctac aaaaaacttc
     2101 aggcaaaaac atttgttttt gcctgaagtt tttttatatt taccgtgttt ttaacattca
     2161 tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt tttgttttcg
     2221 ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag aaaatacttc
     2281 tgcatggatc atcttaacag gtcttctttt ttgaagacga gtatatttag caccagtacc
     2341 attgttatgt tcctctaatc gtctagaaag atcggttgta tatccaccat aaaatgtctg
     2401 atctttacag aataatacgt aaaaataatg ttcatttgcc atagagcatc tcacgaatct
     2461 ctgcagtgta ttctccatcc atattgtaag tgatcaatgg tggagcgact ttgaatccgt
     2521 ctaatttgcc ttctttgatt ccttcaacta acaaaatatt tgcctctttt ccaggttttg
     2581 ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc tgaatgattt
     2641 ctaagaaccg atcaggacga tggaccatag taaagcgtcc atttgttttt agtagcttac
     2701 ttgaaatatc gatcacttct tctaacgtcg tatgaatctc atgacgggcg attgccaggt
     2761 gtttattagg gttcgtttta tttgttggca gccctttgaa ataaggtgga ttacagacga
     2821 ctaagtcaca agaattatgt cgaacgacag ataagctgtc tttcagatcg atggtgtgga
     2881 cagtcatttg ttcatttaaa tggttaagcg cgatactacg tcttgccatg tctgctagac
     2941 gaggttgaag ttcgatagcg tcgatagccg cctgggttcg tttacttaaa aaaagtccaa
     3001 ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca cgggaaaagt
     3061 gagcaagcag cacagcatct aaagaaaaag aaaacacttt ggaactttgg ataatcttga
     3121 tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg aagcatggat tccctccaaa
     3181 atgaaaaaaa tagtcgtctg tattatacta tttttattaa aaagaaccaa ggtggcttgt
     3241 caaacctcta ttttttatgc atactatgac cataagaagg gaaaggaaga gactatgttt
     3301 tatcgattta tgcgcggggt agttcgagta ctattagcct tagttaatgg aaatgcccgc
     3361 tatgagaata aagaagtttt accgcaagat gagaattaca tactagttgc tccccatcgt
     3421 acttggtggg acccattata tctggcggta gctgcagcac ccaaggaatt cagctttatg
     3481 gcaaaagaag aactgttcaa aaatccagtt ctgcgattta tattaactcg atccaatgct
     3541 tttccagtta aaagagaaaa tccaggacca agcgtcatta aaacacctgt aaagattttg
     3601 aaatcgacaa atttgagttt gatcatgttt cccagtggga caagacatgc tacagaactt
     3661 aaaggcggaa tggcattgat ttccagaatg tctaaagcga aaatcgtgcc agctgtatat
     3721 caaggtccgt tgacacttgg ggatctgttc aagcgtcaga gagtgacagt gcgtttcggc
     3781 gaaccaatcg acttatcaga tatcaagaaa acggataaag aaggtatggc ggagatcgaa
     3841 cgacgaacac aagcagcttt tgacaaattg gacaaagaag tgaaccctga tttcaaatat
     3901 gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc ggtcttttga atctactgtc
     3961 ctgttcagtt cagacttttt caatgaaaac gagggagaga cgtcaagtct gccctctttt
     4021 tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag tgttataata
     4081 attagaagga cattttttga ggtgattgaa tgcgtttttt acatacagcc gattggcata
     4141 tcggaaaaaa actacaaggt tatgacttga ttgaagacca gcgtcatgta ctatcttcta
     4201 tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga gacttatatg
     4261 ataggagtgt cccagctgtt gatgccgtag aattatacaa tgaattgatg gtcgattgga
     4321 atttaaagga aaagatcccg atttttgcta tatcaggaaa tcacgatagc agtacgagac
     4381 tttcagcggg tacgccatgg ttcagttatt cgaactattt tttgcgaaca cagctagcag
     4441 aagcatttga accagtcgag tttaaaaacg tacaattttt ctttcttcct tactttgaac
     4501 caattgcagc gagattatat tttgaagatg acaatatccg aacgatcaag caagcggtag
     4561 ccaaggtgat agataaaatg caggagctat ttgattcaga aaaaaaacaa gtattgatta
     4621 gccatttttt tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc gaagtaggcg
     4681 ggttggattc gatccctggt gaactactta caccgtttga ttatgtagca ctaggacatt
     4741 tgcataatca agctgctttg agacaggata atgctcgcta tagcggttcc ccattgaaat
     4801 tctctttatc tgagatcaac caaaaaaaag gcgtatggat cgttgacctg acaacaggcc
     4861 tggagttgaa ttttagggag atcgaacccc tgcatgatat tgaagaaata acagcgagtt
     4921 tcaaagaatt gctggatcct gccttttatc agtccgtcaa tcgggagaat tacatctata
     4981 tctatctcac tgatagaacg atcatcccga atatgatgaa ccagcttcgg caaatatatc
     5041 ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag cgcaaacgaa
     5101 aagtactaga aatgaaagat cctcaaaaat tagcagaagc attttttagt gaaatgacgg
     5161 aagagcagct aactgcaaac caagaaaaat ggctcggaga aacgttgcaa gaaatcatgg
     5221 aaatggaatg atcaagaatg aaaccgaaaa aattacaact taaaaatttt ggtcctttta
     5281 tcaatgagac cgtagatttt tcacgtttga cagaagcacc gctttttttg atcagcggga
     5341 agacaggagc tggcaaaacg acgatttttg atgggatcac cttcgcactt tttggtgaaa
     5401 cttccggaag acttcgctct ggaaaagaga tgcgttcttt gtttgctaca ccagacgaag
     5461 aaacaagcgt gactttttct tttgaacatc agcagatgag ctatgaaatc gaacgaaaac
     5521 cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca gccaaagtaa
     5581 cgttgaccat ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa cgtacagaag
     5641 cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa acagttttct caaatcgttt
     5701 tactacccca aggagaattc cgtaattttt tggtttcatc aagcagtgat aaagagactg
     5761 ttttacgtca tttattcggt actcaatttt tccaacagtt caatgagcga ttaaaagaaa
     5821 aagcaaaaca acagcaacaa aaattggatc atttagagca agagctgcat cttctgcaaa
     5881 aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc gttttagccc
     5941 aatggacaaa ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa gaagctctaa
     6001 cggaacttca ggaaaaacaa aagcaactag aaaattccta ctatcgtttt gaaagcttga
     6061 aaaaaagtta tgaagaaaaa acgatattaa taaacaagca aaaggaatta gctgctcaaa
     6121 aagacgaaat agaggaaaag aaacgatgga ttgggtatta tgaatttacg caacgattag
     6181 tcgaaccaat ccagcgccta caagaaataa aagttgaaaa aatagaactg tcacaaaaaa
     6241 gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag tggcaaaaaa
     6301 atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa aaactgcaaa
     6361 acaaacagat gatggtgcca attgtcgaat ccttacgtag taaaaaacaa gacgcagaac
     6421 aattaacaat ttcattggat aaagaagaat tgaatgccga gaagctgtat caaaatctcg
     6481 ctatctatca ggaaagaact cgaattcttc aagaaaattt actggatcag gaaaaactgc
     6541 aagaagatcg tcttgctttt ggacagttaa aatatctaga tgaaaagcag caagaaatag
     6601 aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat aagagaagaa ctcaaaaaag
     6661 caactgaaga aataacaaaa aatagtcagc tattgaaaga acagacagaa caagcagaac
     6721 agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt ttgcgtccag
     6781 gggagccatg tcttgtttgt ggatcaacgg aacaccccat acaacaatta aaacacgaac
     6841 agccaaataa ggaagatatc ttaaaagtag aacaacaact ggcagaagct gagtctgctg
     6901 tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat cttcaacaac
     6961 aattatcaga tttagaacaa aaaatcatgg caacgaatat acaattggct gaacaagaag
     7021 aacacttgca acaggctttg aaaaaggcat ttcctgagat aacagaacaa aaaacaaaaa
     7081 caactcttgt agtaatcgaa caatctttag atagacgaaa aagcaagatg gccaatgcaa
     7141 gaatagagct ggaagaaata agacaacaag cagaaaagct cgaacaacaa ttagctgtct
     7201 acaaacagca gcagacagaa aaaaataatc agctccagca actgcaagga gaaatcaaaa
     7261 gtttagaaca acaattgcaa gatacggata gtaactgttt agaagaggag atacgacgac
     7321 ttgaaaaaca gttagaagaa gactcgaatc aactagctgt tgagcagaaa gcaggagtgc
     7381 aattgttgca agatcttcat acgcttcgga cacagcttga tcttcaacat gaacaaatag
     7441 aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag ttagaaagcc
     7501 agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa gcacaatata
     7561 aagaaaacca acgtacagtt acttcttatc atgaagagca attagtgaca gctgaccggc
     7621 tgcgtcaatt agaaaatatc cagctaactg agacattccc agatacagaa gagatcagcc
     7681 aacagttaga agaaataaga cgtatgatcg aaataaagca agaaaagata tacggtttac
     7741 aagaacaaaa aattagtaat caaaaaatct acgatgaatg tctttcgatt tatcacacaa
     7801 gccagacgaa attagatgag cttacgcaat tacagcagct ttcgcaaaca ttcaacggag
     7861 aaaatcctaa gaagactagt ttggaacggt atgtgcttca ggtttatctt caagaagtgt
     7921 tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa tttgagctag
     7981 cggataccat tggcagttat cgagggaaaa ccggattaga aatcaatatc tatgatgatc
     8041 aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt attgcagcac
     8101 tttctttagc attagctctt gccgatgtga tccagacaca agctggtggt gtagcaatcg
     8161 atgcattgtt tattgatgaa ggatttgggt cattagatga agaagctttg gaaatggcaa
     8221 tggaagcttt agaaacgatc gaaagtgaag gaagaatgat cggcatcatt agtcatgtaa
     8281 gagaattgaa agaacgagta ttacaacaaa taagaattga tacagaaggt agcggacaaa
     8341 gcaaaatacg ctatcaactt ggataaaaag ggagattggt aactatgaaa tggagtattc
     8401 cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga aggccgagtg ttatcggttg
     8461 tccctgacca agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa ttgtatatgg
     8521 ttgatttgga tggtacagca aaagagatag attactgcca atgtccctat tgggaggaac
     8581 ataaatattg taagcataca gttgctgtgg aactttattt acggtcgaaa aatctatctc
     8641 gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa agcaacaagt gagggagaaa
     8701 tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg gtgcaacctt
     8761 tgattattga ataccaagta gaaacgattg agactaatca gtatcaccca gagctttcta
     8821 ttttggcact ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca tatatcgtaa
     8881 aaaacatcta tgatttttta caagcttaca cagaaaaaga aacttacact gttaataaac
     8941 agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa atactcacgc
     9001 atcttgcagg aagtgctcag acgcatcaat tattaggtac gaacggctta caagtcaaag
     9061 gaaaactaga taaaagatat ctgcttctcc cagtagaaca agcccgcttc ctgttagagc
     9121 agatgaatca gactacacgt ttgcatctgc agataaatga acaaaacgat caaggaatac
     9181 aattttccga tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga gaagattatt
     9241 tgctgcaagc attgaatgat tttgattttt tctttatgca ttatcagtgg ggagttatcc
     9301 aagggacatt ttattctctg acgaaatatc aacaaacggt ttatcaaact tggaaacaat
     9361 tgattcgccg attggagaaa ccagaagtag tgttcaagaa aaaagaatta ccgtctttgt
     9421 ttaaagagat tatccctctg ctgtcagaga tcggcgacgt agctattgcc cctgcagtag
     9481 aagaggaagt agcagatatc cccgtcgaat ttattttctt tttccgaaaa gcaaaaggaa
     9541 aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat ctattcgact gacccgaagc
     9601 atgaggtctc gacagaacag gcttttcagg tgttacgtga cacagtagaa gagaaacgag
     9661 tacttgatct attcagaatg tatcgctacc agaaaaatga aactggttat gaacgtccat
     9721 tgccaagcgg ggaagaacta tatgcctttt tccgcacaga actatctgtc ttccgtcagt
     9781 ttggtgaagt cagacttgga agaaaattgc gagagttgta tttagatgcg cataaacatc
     9841 aaccgaaaat cgaagtaacc gagaccggtt catggctcga tattcgattc gatgtgacag
     9901 ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat gatgcttttt
     9961 atacattaga aaatggtcag gttctttctt ttgattcaga agaatttcaa caaacaagcc
    10021 aagtgttgca acaattacgg gaatcgatac gtacagaaga aggaacgatc catgtaccta
    10081 aaaatcaagg attgatcatt caaaatcaat tggaaaaaag caatgctact tttagtgaat
    10141 cgttccaaac gatggtacaa gatctcattc atccagaaag ataccaggca cagcttccca
    10201 aaggattgaa tgcaacgatg agagattatc aaaagcaagg ttttcgatgg ttgaagatgt
    10261 taggtcatta tcagtttggc ggtattttag ccgatgaaat gggacttggg aaaacactac
    10321 agacaattgc ctttttactt tcggaaaaag aagaacggaa aagcttctct gctttgatcg
    10381 tagcgcctgc gagcttaatt tacaactggc aggcagaggt gagaaaattc gccccttctt
    10441 taagtatcca agtaatcaat gggaataaaa aagaacgtga agaattactg gctaaagaca
    10501 cagatatccg agtgacttct tatgccagct taagacagga tctagctaat tatcagtcgc
    10561 aaaaaattga ttatttgatt cttgatgagg cacaaatggt caaaaatagc agtacgaaaa
    10621 ctgcacaagc attgagagaa ttagctgtac ctcagcggtt tgcactgagt gggacaccaa
    10681 tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac gattatgcca ggatttttcc
    10741 cgactaaaac aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg attcgaccgt
    10801 ttattttacg gcgagataaa caaactgtgt tgaaagactt gccggaaaaa acagagatga
    10861 atctatattc tgcacttaca gaagaacaaa aaactgttta tttagcttac ttgcgtcaaa
    10921 tgcgtgaaga aatcacatca atggattctg aagcattcaa gaaaaaccgg atcgggatac
    10981 ttgctggtct gacaagactg cgacaaattt gctgcgatcc tcgtctgttt attgaagatt
    11041 accaaggcgg ttccggcaag ctcgaacaag taaaagatct actggtagct gccaaagaga
    11101 acaaacgaag agttttgctg ttttctcaat ttacagggat gctgactatc ttgcaagaag
    11161 agttagccga gctagggatc tcgacatttt atttaagagg cagtacgaaa ccacaagatc
    11221 ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg atttccttaa
    11281 aggctggggg aactggattg aatctcacgg gagcagatac ggtcatcttg tatgaccttt
    11341 ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc acatcggatc ggacaaaaaa
    11401 atgtggtaga agtgtggcgt atgatctctg aaggaacgat tgaggaacgc atggattcac
    11461 tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa gaacagttga
    11521 cgaaactgac tgaagaagac atccgcatga ttttaagtgt aggagaaatc gaggagtaag
    11581 agactcgaaa aagcgttcag taatgggtaa gaaagccttt ctaaagaagc gattgtttat
    11641 ttgatttaaa aaatagcaag gaggctggga ttccatttta tcccagcttc cttgctatca
    11701 tttttttatt tctattttta aaggttatac aaagattgtc ttcattgtct ctaacagctg
    11761 atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt tcaattttgc gttctcgatg
    11821 gttgaaccaa agcgcttgcc agttagcact ttttgcacct agaacatcat tgtcgtaatt
    11881 atcgccaacg tataaggtct cttctggaag catatgaaat tctttctcag ctagctgaaa
    11941 aatctctttt tctggttttt ggaaacctgt tgcttgagaa atgagcatat tgcgggaagg
    12001 aatccaattg ttcaattgaa gttgcttcag tttttttgtc tgatggtcag tcggcccatt
    12061 tgtgataatt cctaatggga cattcttttc cttcaagaaa tcgagggttt ttttgacctc
    12121 ctcatgcaag cagatattat caagttcttc ctcgtaaatt ttttgaaata agaggccatc
    12181 ttcttccgta atatgagggt agtctaaatc ttttagtgat tggctgatcc gatgtgctcg
    12241 catatattct agtgtccaat ctcctgccat tacttttggg aagttttcat cactatgatg
    12301 gcggaaccgg atatatagtg gatgcatatc tgcatctgaa acaagcggaa cgactctttt
    12361 gaccgcgttt cgaaatggct gctgctgatc atacatcgta tcatcaacat caaacacaac
    12421 tgatttaatc aaaattttac acgcctttcg gttttcattg attttttcac aaatccttga
    12481 ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat aaaggttttt cttactatat
    12541 ttctatttcc ttgacagaat aaggttttta tatttcagtt tttttcacta aaaatcgtga
    12601 aacacgtcaa taagtttgat gaacaaatta atcattatgg tagaagtcga ttgatttatt
    12661 ttaatgcaat ctctgtggaa ttttcgaaag
//
LOCUS       DACDRN010000067        12685 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDRN010000067 DACDRN010000000
VERSION     DACDRN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.067"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(612..1025)
                     /locus_tag="IWJ80_002196"
     CDS             complement(612..1025)
                     /locus_tag="IWJ80_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP9461897.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(1102..1398)
                     /locus_tag="IWJ80_002197"
     CDS             complement(1102..1398)
                     /locus_tag="IWJ80_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461898.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            complement(1395..1952)
                     /locus_tag="IWJ80_002198"
     CDS             complement(1395..1952)
                     /locus_tag="IWJ80_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP9461899.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            complement(1949..2368)
                     /locus_tag="IWJ80_002199"
     CDS             complement(1949..2368)
                     /locus_tag="IWJ80_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP9461900.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            complement(2365..2610)
                     /locus_tag="IWJ80_002200"
     CDS             complement(2365..2610)
                     /locus_tag="IWJ80_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461901.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            complement(2570..2926)
                     /locus_tag="IWJ80_002201"
     CDS             complement(2570..2926)
                     /locus_tag="IWJ80_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP9461902.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            complement(2926..3231)
                     /locus_tag="IWJ80_002202"
     CDS             complement(2926..3231)
                     /locus_tag="IWJ80_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461903.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            complement(3228..3389)
                     /locus_tag="IWJ80_002203"
     CDS             complement(3228..3389)
                     /locus_tag="IWJ80_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9461904.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            complement(3386..3688)
                     /locus_tag="IWJ80_002204"
     CDS             complement(3386..3688)
                     /locus_tag="IWJ80_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461905.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            complement(3850..4119)
                     /locus_tag="IWJ80_002205"
     CDS             complement(3850..4119)
                     /locus_tag="IWJ80_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461906.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            complement(4131..4880)
                     /locus_tag="IWJ80_002206"
     CDS             complement(4131..4880)
                     /locus_tag="IWJ80_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9461907.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(4883..5569)
                     /locus_tag="IWJ80_002207"
     CDS             complement(4883..5569)
                     /locus_tag="IWJ80_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461908.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            complement(5575..6246)
                     /locus_tag="IWJ80_002208"
     CDS             complement(5575..6246)
                     /locus_tag="IWJ80_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9461909.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(6239..6580)
                     /locus_tag="IWJ80_002209"
     CDS             complement(6239..6580)
                     /locus_tag="IWJ80_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461910.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(6758..7456)
                     /locus_tag="IWJ80_002210"
     CDS             complement(6758..7456)
                     /locus_tag="IWJ80_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9461911.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            7543..8013
                     /locus_tag="IWJ80_002211"
     CDS             7543..8013
                     /locus_tag="IWJ80_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461912.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(8056..8235)
                     /locus_tag="IWJ80_002212"
     CDS             complement(8056..8235)
                     /locus_tag="IWJ80_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461913.1"
                     /translation="MGKFNRALVFSAPLIIYALELWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(8222..8560)
                     /locus_tag="IWJ80_002213"
     CDS             complement(8222..8560)
                     /locus_tag="IWJ80_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP9461914.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(8576..8779)
                     /locus_tag="IWJ80_002214"
     CDS             complement(8576..8779)
                     /locus_tag="IWJ80_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9461915.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(8794..9570)
                     /locus_tag="IWJ80_002215"
     CDS             complement(8794..9570)
                     /locus_tag="IWJ80_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP9461916.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            9869..10189
                     /locus_tag="IWJ80_002216"
     CDS             9869..10189
                     /locus_tag="IWJ80_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9461917.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            10207..10638
                     /locus_tag="IWJ80_002217"
     CDS             10207..10638
                     /locus_tag="IWJ80_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9461918.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            10768..11229
                     /locus_tag="IWJ80_002218"
     CDS             10768..11229
                     /locus_tag="IWJ80_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAP9461919.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            11263..11898
                     /locus_tag="IWJ80_002219"
     CDS             11263..11898
                     /locus_tag="IWJ80_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP9461920.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            12011..12646
                     /locus_tag="IWJ80_002220"
     CDS             12011..12646
                     /locus_tag="IWJ80_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461921.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
BASE COUNT         3713 a         2476 c         1986 g         4510 t
ORIGIN      
        1 taaaactaat ttgaatccaa atcaaatgtg tgaaaaatta aatacagtac ttgataataa
       61 cgattcctta ttcatctgtg aactgcaaaa aaattatcaa ggtagagcta cagaggaaaa
      121 ttggaaattc attaacgaac atattttctc ttagtaagga ttagatattt tttctctgtt
      181 taaacaattg caaataccgt caatatctga attagaaaca cttacctttt gctccttgct
      241 actaccaata gcttggagca attcttgtat ttcttctgga gtgccttcta ctgataattt
      301 catttttctt ccctccaata cataaattaa tagacagcag cagttgaaag acgacaagaa
      361 catgtttagt tttgtgagtg ctgctgtcta atcaaaataa acagcaaccg atgaagaatt
      421 taggaggagt tgaattcaca tccttttctt cataattagt tgctgtctat ctaagaagaa
      481 gtttaaaacg atgagggaga ttgcctccct tcgtttattt tgtcgatcct gtttcctaat
      541 ctttcgacac taccataata acactggtaa atgtctaaaa accgccatca ttccgccaaa
      601 aaaccgccaa attatttata agcaattatt tttccgtgtt tatatgcttc tgcaaactct
      661 attagagctt ccgacttcat ccgttgtata cttctttctg aataacccac ttcacggcta
      721 attctgtagt ttgagaagct atctggcaca cagaagctgt agtagagtat ctgacgacta
      781 atcagactaa gagccatcaa agccgctaga atcgcatctc tctccgtttc tatatccatc
      841 atctgaataa tcgcgtcttc tgccttattg ccatgcttcg gtgccttcgg catatccgtt
      901 ataatcggcg acttaatatc tatcaaagag cgacctgcca tccgctccaa acgccgaaag
      961 ttcttcagca catctctcgc attacatctt gtctgtttga aatctacctc tcgtaacaat
     1021 tgcatcaagt caaaccgctc ctttatgtga tataataaat gtgttggatt tattgaatca
     1081 gtcggagcga tccggctttt tttatttgct cttgattact tccatgtcaa ctaatctcgc
     1141 tacagctaaa taatgcttgt gttttgcagt cgaacgatca cacttcattg tattttcaat
     1201 acgaatgatt gctgagtttg acagtatctt tttaacgtat cctctgaacg gataaacgaa
     1261 ctcttctgct tcacagcgga ccatgtcacc gactttgaat tttgatttct tgcgtgtttt
     1321 agggttcttt gtcggcatat ctagcattaa accgccgata ccgtgactgc tagcgtaaaa
     1381 tccgtctttt agtttcatct ttctaccacc tcttccactg ttccattcac caacaatgct
     1441 gcagcatccg ctgttgcttt atcagtgaaa agcaatcgat gagatggatt gcattcaaaa
     1501 ctataattta ctccatcttt tccccataaa gcacatttag taagatagtt tccatcggca
     1561 ttctttattg cccataacgg ctctttctca acctcgtagc cgttgtatag gctcaatagt
     1621 gtttcatatg attgtcttgt tgcccaagcg agtaattctt ctccttgctc ttgcgtcact
     1681 atgccatctc gtacaaacca atcacagaaa taatatgaat caccatcttt agatttgatt
     1741 aaatacgcta ttttttcagc ttttgtaaaa gggtccgtag atttctcaag ccaaccggcc
     1801 acgagttgtg gaataactgg tttctgcggt tcgtctattt ttttcattaa cgtaatacct
     1861 gaatttatac tctcgttata gcaagcagct attgcatcat ttcctatcgc tttaatactt
     1921 tctaactttt tgattgcttc ctgtttattc atcgctgttc ctccaataac tcgctattct
     1981 cgtatatatt tccggtgact tcgtacgtgt attcttcaaa cagctctgtg ttaaaaattt
     2041 gatactctaa gtcttcatca acagtggcac aaactaatcc ggaataatct attgaatttt
     2101 tgacaataca gactttatta tctaagtaat cgaagccatt tcgcacgctg actaatacta
     2161 tatccccttc aaatatatcc acaccattct tatctttcat tcctgtggat tgcatgaggt
     2221 cgatgtttcg cacgtgatat gatttcaatt cggtaggatt tatccaatat tcaataaagt
     2281 ttgttttgcc gtttttagta aaatgcaaga cggctacatc tctcattacg ttctttcgtt
     2341 tatcccacgc tctaaacttc ggtatcattc gctgtcctcc tcgtattttt caatcaattc
     2401 cattactttt ttcactattt caaactcaac cgcttttgat tcttcgaaat catgaacaat
     2461 ttctggaaac agtacatcat caactaccca caaaataatc tggcgctttc caccaaaatt
     2521 tatgattaga tggtccgatt ccacagatag tgttgctcct gattcgatat catataaatc
     2581 catgctgaat tgaacgagtt tttttatcat ttgctgtcct cctcaaatac tcttctaata
     2641 tttctttata cttctctaca aatttgaaac gatcttgatg aagtttcttg ctccaatttg
     2701 tttgccgatc cagctcacgc atctgatcga accctttttg aatttcgttg taataaaatt
     2761 caatgtttgc tgctgctttc caatgcctgc tacttcgcac tcctgctcct gtttcagcca
     2821 tttctaactt aactaattca gctcgttctt ttgatttttt atctttctga atcttcatca
     2881 tgattttctt gaggatgatg tcactgtatt gtgtaatgag atccattatc tctcctccac
     2941 atacctaaac tgtcgtcctt ttgaatcaat ccataagctc ctagctctat cccagataat
     3001 gtttttgctt aatccagtaa tttcagataa ctgttcagca gtacctgtta ctaaaattcg
     3061 gtcatcatgc cagattgcaa tttttctcgg cgttctccgt ttgggctttt cagtccacat
     3121 tgatttaccg agcttttgga cttctgcaac tatttctttg tcttcttgcc aagattctga
     3181 cttggttaat tcagcaattc ttttcattgc tgctttctta tccacgctca ttcctccaat
     3241 cgatggattt cccttcttaa attctctatg tgcaaatcga ttgcctttct cgccgtttca
     3301 ttgaccatca ctgcctttgt tcgttccaga tcgtcaatct cacgctgaag gcttcgaata
     3361 cgcatttgaa tcacttcttc tgttgtcatg atgattcctc cacgtatcta aacgttctct
     3421 tcttagcgtc tgtgtagcca caccttgctc ttttcctcac gattttctcg tgcaagcctg
     3481 tgagacttgc taactgttca gctgtcccta tcactagaag tttgtcaccg tgccaaattg
     3541 cgatttttcg tggtcttggc ttgttgctct tgtctgccca catcgctctt ccaagcctca
     3601 tcacttctga agcagcttct ttgtcatttt gccaatcttc tgaataagtc aattcgataa
     3661 ttcgctgcat tgccgctttc ttatccatcc cgacattctc ctttcagtaa tttgagtact
     3721 tgatcaagtg cgctctcacg tccgccatgg aacgtgttga gccacttgtc ttcgtacgat
     3781 acactttgtc ttaaagcttc ttgatgcatt agttcaatct gtgctgtaaa tgtctttagg
     3841 tccatctgat tacacctgct caagttcatt aagatgtttt tgcaagcctt taacacaatc
     3901 aacaaatagt aatttggtat atgctaaatt tcttaattgt gttgtatcga tataaagtgc
     3961 gaaatagtat ctgagcttac tccaactcga acgatcattc ttaattcctt caattccagc
     4021 ttcttcgagt tgatcatata cgtctctcag gacgtctaat tcctcgccct ttttataatt
     4081 cgctatttca ttaattagtt ctagataatc gatcttcatt tttccacctc ttagaatggt
     4141 gcttttgatt gtctattagc tcgttctagc gcttttttct tgagataggc ttcttggtcg
     4201 attgcccatt caggaagctt ctctcgtctt cctgtgcgct tgtatccact gcttgcgttc
     4261 ttaggttcac ttttttcttt ccttgcccaa cttcgaatag ttgccaaata gtttttataa
     4321 gtcttaccag atgattcaca atactctgac agtcgttcga ttcgctcttg gtaatcatta
     4381 gggaattctg ttttgagttt ctccatctgc tcatctgaca aaagaacatt tttatactct
     4441 ccgtatttat gacggacggg cttagccttc gattttttcg aaggcggtaa ctctcttata
     4501 tattcttttg tattattaaa tgtattatta atagatgtat tattatcttt gactttttcg
     4561 tcaatagggg tattgcgttt ttcgtcaata gggtattgat taattcgtag gtacctattg
     4621 attatttgat tggtaccctc tttgtaaatg atttcccgat tcaagtatcc aaacttaatc
     4681 aaatcactta cccatcgcga tatggtctct ttattcacac catataaatc tgcaaagtac
     4741 tcattgcctg cccaacaaaa gcctctttca ttacacaagg ccgttatctc tccgtataac
     4801 aacttagtat ttggtttaag tcttttgtcg taccttacgt tggctggtat aatcgcataa
     4861 taacttcgat gttctgtcat ttttaccctc caatatttaa ctttttgatt gttttctggt
     4921 ttaatttgat ccctttgatt tgatatttat ttttgaaatt aatcacacct attttgtgct
     4981 tctctgtgtg atggattctg cagagtgctg caaatgtgta ctctgcatga tcaacttctt
     5041 tgcgctttcg tcttcctagc gctttgtcaa agtgatcgat gtcagctcct gttttgccac
     5101 agatgcagca gactcttttt gtgatgcatt tgtagaagta atattcttga ttcgcaggta
     5161 aaatctcata gccttctttg aaaggaatat gatgttcaaa gatgaaatct aagatgatat
     5221 ttgctaagac attggcatca ctcacagttg tattcgattc gtctttcagg cttattttgc
     5281 gccctgtgac accttcaaaa cgaaagtaga agaattcctt ccagaagtcc gttggcatgc
     5341 ctgtatcgat aaaaatatcg cctatcagcg catagatgaa gtttcgttgc tgtacggtga
     5401 aacgtctagg atcaataaaa cgaatttcaa tgactcgatc accatcgtag ccgtcataca
     5461 tcgtctttag tcgatcaatg ttcacttcct cattgatggt tgcgcttatg tcttttcctt
     5521 tgaacttttt cagaaccgct gaatatgaat cgattaatgg tttaaacact catatcactt
     5581 cttatctaat tcttttctct tagctgctat tgctcgctcc atcaaggcac attgctcata
     5641 gcttaactgt tcaatagttt caacgttatc agctaagagc cctaatttat ctgtctgctc
     5701 attaacatat tcgattaagg ttttggtcat atctttaccc atctgctcat tgaaagcttc
     5761 tagaatcgtc tctagcatgt ttaatttctt tgtatcaatt ctaggtggtg ttggaatatc
     5821 ttccccttga aatacatata atcccagtcc gtgtagagcc aatgctttca caaagcatcg
     5881 cttcaatgag ttattgattt gcattgcatt tggtttaaca actggttggt ttcgataatc
     5941 taaaacagga aataattcgg tttccgtgtg tcctttaacc gttactgaga cagatacata
     6001 agtcccagtt tcatccataa gaaaaggttt atattcctca acaagaaagt cttgatgagt
     6061 tccagaaaca accctgtagt gtttgtactc attaatagtt accgttgcct gtggatcatt
     6121 ctttttcata atctcccacg cgtgagccca agataaataa tcaaaatttc cttttttctt
     6181 gagaatttta tttaacttac gactaaaaag tttttcaaag ttcgttgtcc ctttgatttc
     6241 actcatcaaa ttctgcctcc atttcagcaa tgtatttctt acctggtccg taataagaga
     6301 tatcaatcaa gttatctctg tcgtactctt ctagcgcatc aatcaagcca tcttcgatga
     6361 cgtaaatata ttcaggttta ttcgaatgct tcgatagatg gataagataa acatgatccc
     6421 aaatagttac atggttgcct aaatcatctt gatcccaagc tagttcttca ttcgtcaaaa
     6481 gatttcgtct gatttttcga ccacttgttt cctcaatttt cggcttgccc caatcaggat
     6541 caatcaaata ttgatctaga gtggaaagtt ctttttccat atgctaaaat ctccttatga
     6601 tgtgttttct ttgtgactct ttgcttgccg gcggagtcac ttttttattt gttgccatgc
     6661 tttttgcttg tcaatatgtt gttggcttag gatgattggt ttatagtatt tccaccagca
     6721 attagcaatt gccgtcccta ttcttagcgc ttcagctcta ttcattgtca tcaccaaaaa
     6781 gtctctgttg tctgttcagt tgatcaattt ccatacggat cgcagtttct ggcaaccaca
     6841 tttcaataaa tgaaacagca tcatcgaatc tcttacgagg taactcgcca tatcttggga
     6901 ttgaaaaggt acgtttaaat tcagaccaaa attttgagaa tacttttttg ctgatttctt
     6961 cataagctcg gctttctttt ccccctagaa ctcccataac tttcatattt cctttttgct
     7021 taatttcaaa ctcttgttgt ccgctaattc gcatagtatc tttaagcatg gaaacatctt
     7081 ttttaacatc tttcatttct tctaattgat agatcatcat atcttcaatt gtttgaggaa
     7141 cagtattctt gcgaataaca tcttccattt cgttgaatgc ttcaatgtat ttttgtttga
     7201 agtagatagc tttctttcct gtaaacccca tagccagcaa gaaaaatcca tctctactaa
     7261 tgaagaaaac tcgtcgattt ctgccatatg aatctggttc attaccttct acaaacatct
     7321 gtccaaaatt ggacacatct ttttttagtg catcaatatc tcttaaaaca tgttgatgtt
     7381 ttttctcgaa gctttctgcc acttgtaagc tcgtagtcac agcttcttta tttttcaaaa
     7441 ttactaattc ttgcattatt tcttctctcc tttttggtat aatttagtta aaaactggtg
     7501 gtgtttattt tgaatttttt tcacttaaca atttttcaat ggatgtcaat aataagtttt
     7561 atattgagta cgtttttaac agggataaaa ataaaaggaa tgcgccctca actagaagtt
     7621 gaacttaatg cttcttactt cgctgcagat atgatttata caaaagtaat catttctaac
     7681 tattcaacag aaccagctat gttagttaat ttagaacttt catccgctaa cttaaaccgc
     7741 agatggtcag ctactccatt taagaaatta attgctaaag gaggatcagt cgatgataat
     7801 agaatatact ctgaagctgt accgctaaat attcctccta aaagtgctat atcttgttac
     7861 cttgcatttg aagtagggaa aattaatttt aataaactcc ttaatcacca aacaaaaatg
     7921 atatttactc taaatagaac ccaaatccac aaaattgttg atatcaaaaa tacaaacttc
     7981 ccaatagaga aattagtgaa agaattaaac tgagtaatag taatatacat tctagtttct
     8041 cttttctatt catttttagt cagccccctc ggttggcttt ttcgctctgt actcagcttc
     8101 agcttcatca agccccataa aaatccaaac catgtaaaca atcgttccta tcaacgcttg
     8161 cctgcttccc caaagttcta aagcgtagat gattagcggt gcgctgaaca ctagcgctct
     8221 attaaatttt cccatccgct tgcctcctta agatttccga aaaatttgtt tctaaaaatt
     8281 caagtgtttt acttcttaaa aatagatatg tgtctcttcc ttcaactgga taatagacaa
     8341 atccattttt gtttttctcg atatcgataa tatttctata tcttgggttc tttaaaactc
     8401 tagaagtaaa ccaatcatat tttctgttaa tccgttctag cacttctggc aacgtcatcc
     8461 atctaccagt atcatcagct ttttttaact cctcataatc cacttgggag ataattacat
     8521 agccttctgg aattgggatt tttgcttcta gatattgcat tagctgttcc tccctttaca
     8581 attcgtacat agtgataatc gaatctataa ttctgtttgc ttctgcagaa gtctttttac
     8641 cgtttaaaat taaagataag tagcttttgc taattccaaa tctttcagca agcatggtgt
     8701 aagttaagaa ctttgaactt tcgacatatt ctttgatttt ttctctatct cgttgagtga
     8761 tttctgcaat gtcagtcata ctaaaactcc tttctaacca atttcctcta aatccatttg
     8821 agggtaatat ccttcttttt ttagtaattc gtagataaat agacgccctt tctgtgtcca
     8881 tttggtattc attacaattt tagtgccacc atcggctttc gggatctcag ttgtatgaga
     8941 ttttgtgtat ccttggttca tgtgtttttt gcacaataac cattggttac cgactttttt
     9001 ctgaatacct agtttatgaa gtaatttatt catctgttgt ggagacatcc cataatctgc
     9061 tgcaatctga ctaattgtta ctgaatctgt agaagataat atgctatcta aataggagat
     9121 tttaggttcg tactctgcaa tcttttgttc tgcgattaat cttccagttc tttcttcttt
     9181 tagttgagtt gctaattgaa tgattgtatc tggattaagc aaagcttctt ctactttttc
     9241 tggagttaga taacctccat gttttctaat tgctggcaac acttcacttg ttacccaacg
     9301 tttaaatttt ttggcagagg gaagttttga ttttaagatt aaactgtaca aaccagattc
     9361 gttgatgatt gtcatctctc ttgattgacc tgaggtcgtg attcgcgacc ccatcttatc
     9421 ttccaaatct acatgccgcg acaaagcatc tttagtgttt gaataaccca aaacactcgc
     9481 aacgtctttg cctacaaaat atggttcatc atttactaaa attgtccgaa cttcgttttg
     9541 ttcgaaatta aaaatttgcg gtgtgttcat atttctcatt cctttctttt atattcgtaa
     9601 acaaatttaa caactttttt ctaaattcaa ttgacagtat ctgagtaata ctctatacta
     9661 agagcataat taaataagac ataaaaacat tgattttaaa gctttcttgg cggtctgcat
     9721 ttacatatca atagtgtttt ttgttgtctt tttagttgtt aaatctgttt acaagatata
     9781 gtatagctca ttactcagaa ttagtcaaat tatttctgcg tatttttcta aacttttttt
     9841 gtaaacattt agaaaggttg ttttatcgat gaatacctat gaaataataa aagacttggc
     9901 gaagaggaaa aagatatcta tacggcagtt agaaattaat tttggatatt ctaacggtta
     9961 tctagcaaag tggaaaaata atactcctaa ttcagatgaa ttaagtcgga ttgctgacta
    10021 tttcgatgtc tctgtagact accttctagg tagagaagaa agagaaaccc ctaaacatgt
    10081 ggatttatca gaagacgata ctgttttttc ttttgatgga aaagaaatat ctaaggagac
    10141 aatgcgtaaa gcgattgcaa ttgctaaagc tttagaggaa aatgaatagt tggagtgatg
    10201 gtttgtatgt atttgaaatt gaaagaaatg ttgagcgaat acaatttgaa agtagtttat
    10261 atggagatga aagagcctgg tttctactat ccaaaaccca gaatcatttt tttgaatgaa
    10321 aatctatacg gtgaaactgc agaagccttt catttatctc atgaacttgc acatttcagc
    10381 gcctctcatt ttgaattttc agttttgtac gatacttcta caacttttca ctcaaaattt
    10441 gaaacagaag cagataaaat agctattcta attttattaa atatctatat tgagaacgaa
    10501 ctgactgatg aatctcagtt taatctcgaa aaatttatgg agtattattc tattcagaat
    10561 aagctcagat acacttgtta tgctgtctgc caatgctatt ttaagaaaaa atattcttac
    10621 gcaaggcaat atgtatgaat acatcaagaa ttattaaaaa aaccgatttc agcaaaacat
    10681 taaaaaagcc cgtgctgcaa cacgggctct ttcctcatat atgagcttct acaaaaaaaa
    10741 tcatatcaca gaaatgagga agaaaaaatg gaaaaagaga ttttaataaa atctccagga
    10801 aaaaccttga ttaaagtaac tgatgattct atttcaatta ttagaaaagg atttataaat
    10861 ttagttaatc aaggtattaa aggagaaaaa actatcccct ttaaaaatat atcagccgta
    10921 caattaaaaa aaccaggtat gagtaatggt tatatacaat ttaccttgct aggcggaaac
    10981 gaaagtcgcg gaggtatatt agcggccacg aaagatgaaa acaccgttat gtttacaaaa
    11041 aaatattgga atgaaatgga aaatttaaaa aaatatatag agaagcaaca atctatatta
    11101 gataataatt ccaaagaaac acaaatttca agtgcagatg aaattaaaaa gtataaagag
    11161 ttgttagatg aagggattat taatcaagaa gaattcgatt ttaagaaaaa agaattgctt
    11221 ggtttataat attcaatatc aaaagagaaa gaggatctat taatggacgg tatatttggt
    11281 tttactggta ttgttttatt tattataggt ttaatcatgt taattgtaag atttatcaaa
    11341 aaaacaaata agaaaacacc tatgattctc cttattatcg ggattatttt tactaccgta
    11401 ggtttctcgc tttcctcatc aaacgaggca aatgacaata acaaacagga aagctcatca
    11461 tctgatactc aaactactac atctaacagc agcaataaaa gcctaactca atttgaagaa
    11521 tttttagaga caaataatca tgactggggt gctttcttag atagttatta ttctattact
    11581 ccagctactg agcaaaatac agcttttact aaatatatta gtggtaaaac atatactttt
    11641 gaaggaactg ttatcgaatc aatgactact agaatagcca ttatagcaga caaagagtac
    11701 gatgataaaa gttggaatga tatttctgca acacctaaag tttcatatgt aatttttgcg
    11761 aaggacttaa acaatgctga tacctttaca aagggcgata aagtaacttt tacaggagaa
    11821 attagttcaa gaggatcgaa catagaaaaa tcctatgctc aatgggatat gattaatagt
    11881 aaagtaagta aaaaataaat gaaccacgcc ccactaagag ttagattgtg cacaataata
    11941 aaacctatac aaaagagtgc catcaggagt cacaatgctt gtaaaggaaa tgacaaaata
    12001 ggaggtaact atggataata gcaatgaaaa actaaccaaa aaagaatatc ttgaattaat
    12061 agttgaaggc ggtattggag caattcctta tattggtggc gttgtgcagt cactttattt
    12121 tgggtctaaa aacgagaagc gtttcaaacg catcgagaaa ttctacaaaa atttaaacga
    12181 atcattagaa aacattcgcg atcaaattcc agaagatgtt ttcaacttag agaacaagga
    12241 ccaactcatt ggaatattcg aagggataaa tgacgaggta gaaaaatcaa aggcacaaaa
    12301 taaaatagat atgtataaaa atctttataa gaattgttta cttcgaatta acaacgcttc
    12361 atgggataac gaggagtatt atcttcttgt actgaatcaa ttaacatcga ttgagattca
    12421 attgttagca tgcttaatga gtcgtggaaa cgaaaatttc actggaaata tcagcatgtc
    12481 tggctattca caagagctaa tagatggtag cttaaataga ttatctgatt ttggtctgtt
    12541 agaaaaaacg attagctcca tagtattagg tgatgtcggc aaacaaaata tgggctacaa
    12601 aatatctcat ttaggaattc aatttattaa ctcaactctt acttaagttt tctttctaat
    12661 atgacatcta gacctatcag caatg
//
LOCUS       DACDRN010000068        12141 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDRN010000068 DACDRN010000000
VERSION     DACDRN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.068"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..95)
                     /locus_tag="IWJ80_002221"
     CDS             complement(<1..95)
                     /locus_tag="IWJ80_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9461922.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVA"
     gene            complement(107..595)
                     /locus_tag="IWJ80_002222"
     CDS             complement(107..595)
                     /locus_tag="IWJ80_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9461923.1"
                     /translation="MFLKVGTGIAYMKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDYEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQEIKMTILDEVHVVNEEED
                     FF"
     gene            complement(672..3290)
                     /locus_tag="IWJ80_002223"
     CDS             complement(672..3290)
                     /locus_tag="IWJ80_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9461924.1"
                     /translation="MKKSAKDRVFDELIRLTGNMSSVTAEKISKNLGISRQNASHYLT
                     RLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQINEAFHSVVGHDGSLREVIQKCIAAV
                     KYPPNGLSVLINGATGVGKSFLATKIFEYAIHEQIIEEDAPFAILNCADYADNPELLS
                     ATLFGYKKGAFTGAEKDTEGLLATANNGYLFLDEVHRLSKENQEKLFVFIDTGNYRPV
                     GENVNWHSAKVRFIFATTEKSEDYLLDTFDRRIQISVMLPTFEDRPIRERLELIQLFF
                     QNEADILQKDIIVSKEALSLMLSHPFSGNIGKLKNIIKISCADVYSRTREEPLTIGKN
                     EILIQLNMLESEVESLPIASLLIKKNQKIKTDRIKPFDYDSILEKITQLINEGNFNEK
                     FSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMFRKVWVSVLGKTYGLTTSGPVAEIISR
                     SYVISPSYKTDLTGINRTLAANLPRTTYLCNQFLEHLPPLSEKYENFLRLLIVVTLSD
                     YVDESIELKGLLLAHGDSTASSIQAVVNQLCGNYVFEALDMPVESNVSEIVESTRNFV
                     KRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGELLLLNNLTTSIALDIGLKMTMNAPFK
                     QIAEDAADKYSINVQYFEGFSQSSNIIISCMSGLGISDKLKDVFRHHLSDESIEVFTK
                     EYRELRELIDQNDETYFDKTKLVITTSDLPRSFSIPNINVYDILDGDGAANLKHILSS
                     EMKPVEFNRLIQELIKFFSLEGIADRLNFLNPTIVMSEVETVISKYENYYHFTLNGRV
                     KLNLYMHTALMMERLFLARTEKAEEPNYDMKPDEDEFYYVSRSIFQSMEMKYNFKVNA
                     YELSLLYELLGPYIQK"
     gene            3773..3988
                     /locus_tag="IWJ80_002224"
     CDS             3773..3988
                     /locus_tag="IWJ80_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP9461925.1"
                     /translation="MKKFIIPCIFLFLSILLFSTSILVGSYLDSSGMLIEPAFFCVPL
                     GYLTLGLAFFTALYSFVKQRLLKKYSA"
     gene            complement(4229..4415)
                     /locus_tag="IWJ80_002225"
                     /pseudo
     CDS             complement(4229..4415)
                     /locus_tag="IWJ80_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252699.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            4849..5808
                     /locus_tag="IWJ80_002226"
                     /pseudo
     CDS             4849..5808
                     /locus_tag="IWJ80_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6339..6749
                     /locus_tag="IWJ80_002227"
     CDS             6339..6749
                     /locus_tag="IWJ80_002227"
                     /inference="COORDINATES: protein motif:HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9461926.1"
                     /translation="MYTIILASHKNLAKGMKETVEYILGPAPFIKVMTAYTDDNYDIE
                     KDTNILLDGTDKVLVITDLLGGSVNNHWMNYVYEKKLTKKITVIAGMTLSLIMELSMN
                     IEDYKLREKISMIIAESQKSIINCSELMEVEDND"
     gene            6742..7197
                     /locus_tag="IWJ80_002228"
     CDS             6742..7197
                     /locus_tag="IWJ80_002228"
                     /inference="COORDINATES: protein motif:HMM:NF015769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9461927.1"
                     /translation="MIKLLRIDDRLIHGQVAMAWTKSVSADYIIVVDDASANDKMKQM
                     ILSLAKPVSTGLDIITSADFLSTFEKRKNQNLMIVTSSPKSAFEIVNAIPGEVDSINI
                     GGLRYAEGKEKVNEYVAITSEDKNYLAKLKESGFKLIMQATPTGKAKIY"
     gene            7214..7945
                     /locus_tag="IWJ80_002229"
     CDS             7214..7945
                     /locus_tag="IWJ80_002229"
                     /inference="COORDINATES: protein motif:HMM:NF015566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9461928.1"
                     /translation="MIECILIGIVGYLTTIDERYFGASMMNRPIIVGPVVGLILGDLH
                     QGILIGAALEAMFIGIVTIGAALPPDVGVAGTIATALAIKSGAGADVAVTLAMPFAVL
                     AQGLNMLAFTINSFTVSQGRKAIENRKLSSFQKWHFFPLIVRLPSAVLTFLVVYFGAN
                     ATESFVNIMPEKLMTGFTVASGLLPAVGFAMLIQGMIDKKSVPYFFIGYLLAAYLGVP
                     VVGVAVAAIMVALLVIQTKRNPVNI"
     gene            7962..8774
                     /locus_tag="IWJ80_002230"
     CDS             7962..8774
                     /locus_tag="IWJ80_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9461929.1"
                     /translation="MGDSKLTKKEINKVFWRSFALQGSESYTDMQGMGYSFSILPALN
                     KIYQGNDEKYYEAVYRNTEFFNTTPHISTFIMGMSLALEEENAKTDQFETETISSIKA
                     ALMGPVAGIGDSFYWGTFRVIAAGIGLSLAAQGNILGPILYFSIYTLLHFLGRYGCLR
                     IGYRMGANAIEKAFENNYLESFSLAASIVGLMSIGAMTSSLVRFTIPAVFKSKGGEIN
                     VQQMLDDIFPGILSLGLTFFVYFLLKKKVKVLWIILAIFVFSIVGVYIGLLG"
     gene            complement(8990..10273)
                     /locus_tag="IWJ80_002231"
                     /pseudo
     CDS             complement(8990..10273)
                     /locus_tag="IWJ80_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(10348..11759)
                     /locus_tag="IWJ80_002232"
     CDS             complement(join(10348..11204,11204..11759))
                     /locus_tag="IWJ80_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9461930.1"
                     /translation="MAKYPFELKLKIVKEYLSGVGEYRHLANKYGISSKSQVSDWVNA
                     YREYGEEGLLKKHQNQKYSVQFKLNALELYQTSEMSYREVASTLEMNNPALIANWMRN
                     FREAGIEGLSKEKGRPLTLTRKKENKKENTTTEERERIKALEKQVRSLQIENAFLKEL
                     RKLRKQEAQQRRKNQSHESSPASEDPFKLVELLETLKFPKATYMYWQKRFDRPNPDQE
                     IEEKMLEIRKEHKDYGCLRLKKELENQGIHVNKKKIQRLIKKLGIEVKSYTRKSRRYN
                     SYHGKVGTVAKNRIHRRFYTNICHQKITTDTTEFKYYEVDTTGVVRQKKLYLDPFMDF
                     YNSEILSYRISEKPNALAIMEGLEEAIQMTNDCPFRRTFHSDQGWAYQMNAYKNKLKQ
                     HKIFQSMSRKGNCLDNSPMENFFGLLKQEIFHGEVYRSLDELKTKIDQYIYYYNHKRI
                     KKKLNWRSPVQFREAVKTAV"
     gene            complement(11848..11931)
                     /locus_tag="IWJ80_002233"
                     /pseudo
     CDS             complement(11848..11931)
                     /locus_tag="IWJ80_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3715 a         2335 c         2001 g         4090 t
ORIGIN      
        1 gctactacta acccggttaa atttagactc tttgtccaag tcatcgctac ttgtccatga
       61 attaatcgtt catcaattcg cactgcttta atcatttttc ttcctcctaa aaaaaatctt
      121 cctcttcatt tactacatgt acctcatcaa gaatggtcat ctttatttcc tgttggcatt
      181 tatttaccag ttcctgaata tcctcctttg ttaattcctt ggttgccaat actatttcga
      241 gcagaattgg cagattaaag ccagacaaaa tagttacagc aatctctttc tccaaaaagg
      301 ttttggtaat cttctgattg acacttccgc caaataaatc ggtaaaaaca aaaatctgat
      361 cctgctcgtt cacctggttt acaaatacat tcaactcctg ttcataatca cactcatcca
      421 cataagcatt aatcaccgta atctcttgaa ccttccccgt cagtaattga atactgctaa
      481 gaaagcctga agctaagtga ccgtgagtcg ctattagaat tttcttcata tggttaattc
      541 ctttctttct gttcactttc atataagcaa ttcctgtgcc aacttttaaa aacattggtt
      601 taatcacgtt aattcacata aaaaaaagtg tctaacctga aaaaatcaga ttagacactt
      661 ttttagacac tttatttttg aatatacggt cctaataatt catagagcag cgaaagctca
      721 taagcattga ctttaaaatt atatttcatc tccattgatt gaaaaatact tctcgaaaca
      781 taataaaatt catcttcatc tggtttcata tcataatttg gttcttctgc tttttctgtc
      841 cgcgccaaga ataaacgttc catcatcaat gccgtatgca tataaagatt tagttttacg
      901 cggccattta aagtgaaatg atagtaattt tcatatttag aaatgactgt ttccacctca
      961 ctcatcacga ttgttgggtt cagaaagttc aaacggtctg cgattccttc caatgaaaag
     1021 aattttatca gctcctgaat caaacggtta aattctaccg gcttcatctc agaactcaaa
     1081 atatgcttca aattggctgc tccatcacca tctaaaatat catatacatt gatattagga
     1141 attgaaaagg aacgtggaag atccgaagtc gtgatgacca attttgtctt gtcaaaataa
     1201 gtttcatcat tttggtcgat taattcacgt agttctctgt attccttcgt gaaaacttcg
     1261 atggactcat ccgataaatg atgacgaaaa acatccttca gtttatccga gatacccaat
     1321 cccgacatgc aggaaatgat gatgttgcta ctttgagaaa agccttcaaa gtattgaaca
     1381 ttgattgaat acttgtctgc tgcatcctcc gcaatctgtt taaatggcgc attcatcgtc
     1441 atcttcaggc caatatcaag tgcaatggag gtcgtcaaat tattcagaag aagtaattct
     1501 ccttttaaat cgttcttgat ctgtgtgtag atttgattta aagagcccat atctacgatc
     1561 agcactagac tatctgtatt cacttgccgt ttcacgaaat tccttgtcga ttcgacaatc
     1621 tctgacacat tactctctac tggcatatcc aacgcttcaa aaacataatt gccgcataat
     1681 tgattcacta ctgcttgaat gctgctagcc gtactatctc cgtgagcaag caacaggccc
     1741 ttcagctcga tggattcatc tacataatca gataaagtca cgacaataag cagtcttaga
     1801 aagttctcgt acttttctga gagcggtggt aaatgctcca agaactgatt acataagtaa
     1861 gtcgtccgag gtagatttgc agctaatgta cgatttatcc cggttaagtc tgtcttatag
     1921 cttggggaaa tgacatagga tctgctaata atttctgcaa caggtccact cgtcgtcaat
     1981 ccgtacgttt ttcctaaaac ggatacccag acctttctaa acatctgaat catcaatttt
     2041 ctactcattg ataaaaaaga atccttatca atgtgatgca ccaaatgctg tacttcattc
     2101 tttatcgaag aaaacttttc attgaaatta ccttcattta tcagctgagt aattttttcc
     2161 aaaatcgaat cataatcaaa cggtttgatt cgatccgttt ttatcttttg atttttcttt
     2221 atcaataaac tagcaatcgg caagctttct acttcggatt ccagcatatt taactgaatc
     2281 aagatctcat ttttcccaat cgttaatggc tcttcccgtg ttcgactgta cacatccgca
     2341 caactgattt taatgatatt cttcagctta ccaatattcc cactaaaggg atgagataac
     2401 attagagaca aagcttcttt tgaaacaata atatcttttt gtaaaatatc cgcttcattt
     2461 tgaaaaaata attgaatcag ctctaaccgt tctcgaatgg gacgatcttc aaaagttggc
     2521 aacataacac tgatttgaat acgtcgatca aaggtgtcta ataaataatc ttcacttttt
     2581 tccgtcgtgg cgaagataaa acgaacctta gcagaatgcc agtttacatt ttcaccgact
     2641 ggacgataat ttccagtatc aataaaaaca aagagctttt cttgattttc tttcgacaaa
     2701 cgatggacct catcaagaaa aagatagcca ttgttggctg ttgccaataa cccctctgta
     2761 tccttctcag cccccgtaaa ggcccctttc ttgtatccaa acagtgttgc cgaaagcagt
     2821 tcgggattgt ccgcataatc ggcacaattt aaaatagcaa atggtgcatc ttcttcaata
     2881 atttgctcgt ggatagcgta ttcaaaaatt tttgtggcta aaaaactttt tccaaccccc
     2941 gtggctccat ttattaatac actgagtccg ttaggcggat atttgacagc tgcaatacat
     3001 ttttgaatga cctctctcag acttccgtca tgacccacca cagaatgaaa ggcttcgttt
     3061 atttgctccg tattatctac aaccgcatat tcatctaaag gcttccacaa aacaggcttt
     3121 cccggtaatt tctcgacctt tttatcttcc actaatctcg tcaaataatg gctggcattc
     3181 tgacgagaga tacctagatt cttcgaaatt ttctcggcag tgacagaaga catattccct
     3241 gttagtcgta tcagctcatc aaaaactcga tctttagcgc tctttttcat tttcccctcc
     3301 agttcaacgt gtctagaagc ataataaact atcggctaca atatgtctat tctatatttc
     3361 tgttccaatt ttactttgaa acaaaaataa tcgagcttaa actgatatta gttataaaac
     3421 tatagttaga ttaccaatct tcctctcata caataaactt ctctcacctg catccaacct
     3481 acttatgtga aagaataact gccgattatt tatctttttt acagttactt tcgaacataa
     3541 aaaaatcacc ttcaaatact agaaaaacta ctatttgatg gtggtttaga acaatttccg
     3601 ttgattcagt atcgtctggt cccacatcct ctgtctcatt aatcacaata gcatactcca
     3661 aaggataaag ctggttgata tctcaatgcc tttcacacct atcaattttg tcacacttat
     3721 cttttcctag gttctattct atataatcta gaaaaacaaa gaaggagaca ccatgaaaaa
     3781 attcattatt ccttgtatat ttcttttctt gagtattttg ttattttcaa cttccatcct
     3841 agtcggttct tacctcgatt caagcggaat gctgattgaa ccagcgttct tttgtgttcc
     3901 gttgggctac ttaacattag gacttgcttt ctttactgcc ttatacagtt tcgtcaaaca
     3961 aagattattg aagaaatact cggcttgatt tgcacgtaaa tcctctgttg aagaaaagtt
     4021 agttttttca acaacgaacc ttcgagtatt aagaatggat gttgatgtag acatcaaacg
     4081 tctgcataaa cattcatttt attcctccat taccaacaat tattcccaat cgccaaaaga
     4141 agcttttttc ggaattacct tctccatttt ttcacctgct ggttatattg tcttttgtaa
     4201 taccgatagt cctcttcaat cattttcaat atgcgaaaag aaccaatttt aacatggcac
     4261 tcaaatcaca attcctaggg cggccaaaaa gatacggctc gttgatttca agggtttcaa
     4321 cgagttcatt gacaaaccca gcggtgctat cttcttttgg aataagaagt tgtgatatct
     4381 aaagtgagtt gattcatgtt ataatttgta tgcatgattt tgatcctttc aagggttgaa
     4441 tgatttagag tgcgagtatc ctctcgtact cttttatttt acctaaaaag ctccagaaat
     4501 gacgaatgac tcgtcatttc tagagctttt ttcagagaat tatgtcacaa tctctttttg
     4561 ttatattgat attctatatc tttttttatt ggttttattc ccttgttcgt tatagactaa
     4621 aatccttctt tcagcattat tcaaatcaaa tggcaacata atagagaaat ttatagtatg
     4681 tataatcatc tgaattcaat caaatcaacc ggagtcatat acggagtcaa actatcccac
     4741 tgagagttta ttaaacgcag attgtaaaat taagctagac aactaaaaaa gtcatttgtg
     4801 ctacactcaa aatagttccg accaaagaat tataaggagt gaagacaaat gacctatacc
     4861 catcttacaa caaatgaact cgtgatgata gaagcctatt tcaatatgca tcaatctgtt
     4921 accgtagtcg ctaagacctc aaatcgttcc agacagacca tctacaatgt gtacaacttc
     4981 ctgaaacaag gccaatctat ccatgactat taccaacaat atcaaaaaaa taaaacacgg
     5041 tgcggccgac gacccatttg tttgccagca gaacaagatg cgtatatccg aaacaaggtg
     5101 gcgcaaggat ggacacccga tgtgattatc ggtcgtgctg agttttcaat ttcatgttct
     5161 gttcgaaccc tttatcgcaa gtttaaaaca aactaatttg attgctcgaa actacccatg
     5221 aaaggaaaac ggaaacccaa cgggcataaa gaacgtcgag ggaaacaagc cttcaaacgg
     5281 aatatttcag aacgtctcac ggactatcct gaatttcaac aggaatttgg acatttggaa
     5341 ggcgacacta ttgtgggtgt ccatcataaa agtgctgtca tcacgctggt tgagcgcttg
     5401 tcaaaagtga ttattacttt aaaacccgaa ggacggaaag cactagatat tgaaaaagtg
     5461 acaactgact ggctacaaaa gcttccccga aacttgttca aatccattac ctttgattgc
     5521 ggaaaagaat tttcgaattg gaaaaacatg agtaatgcta acgatatttc tatttatttt
     5581 gcagatcctg gaacgctgtc acaacgggga ttaaatgaac attctaatgg attgttacgt
     5641 aaagacggac ttcctaatac aatggatttc aatcaggttg accaaaaatt tgtggcggcg
     5701 gttgcctcca aaagaaatca tataccaagg aaatctttaa attatcgaac atcgttagaa
     5761 gtatttttga gttacataag tgaggagcaa ttgtctaact taatttgaca tttagaaaat
     5821 aataaaccat atcccatcta ttcaaataat attttaccga ttgaaagact tgctaatcaa
     5881 agcagaatag tttatataga taaattattt tctttaaact tatactcatt tttagatatt
     5941 ttataagaat acttcatgtc ttcgccagta atataaagaa catctgtctc tccatttgga
     6001 cttgggttat ctattttggg tgtaacatac tatacataca accgatacat aaaagatttg
     6061 acatctggac aaaacacaat gctttacttc atttttatag gctttgttta caccatcaca
     6121 aaaatcacct cctaatttac aaattcatta aacaaacaga aataaaaaat caccacgata
     6181 gccgcgttca aaaaaattta caaaagagaa ttttagttaa agtttttaga ttcgccccac
     6241 taattaccaa aggtatttta tttattctta atacgaaata atttattgac atttagatta
     6301 ccaatggtat attatttata aataaatgga ggtggattat gtacacaatt attcttgcaa
     6361 gccacaaaaa tttagcgaaa ggaatgaaag agacagtcga atatatcctt ggccctgcac
     6421 cattcataaa agtaatgact gcatacacag acgataacta tgatattgaa aaagatacga
     6481 atattcttct cgatggaact gataaagtcc ttgtcatcac tgatttactt ggtggtagcg
     6541 taaataatca ttggatgaat tatgtttacg aaaaaaaatt aacaaaaaaa attacagtaa
     6601 ttgcaggtat gactttgtcc ttaattatgg aattaagtat gaacattgaa gattataaat
     6661 tgagagaaaa aatttccatg attattgctg aaagccaaaa gagcattata aactgttcag
     6721 aattaatgga ggtagaagat aatgattaaa ctattaagaa ttgacgatcg cctgatccac
     6781 ggtcaagttg caatggcttg gactaagtcc gtatctgctg actacattat tgtagttgat
     6841 gacgcctctg ctaatgacaa aatgaaacaa atgattttat cattagcaaa acctgtatca
     6901 acgggactag atattattac ttcagcagat tttttaagta cctttgaaaa aaggaaaaat
     6961 caaaatttga tgatagtcac tagttcccct aaaagcgcat ttgaaattgt aaacgctatc
     7021 ccaggggaag tcgattcaat taatatcggt ggtttacggt acgccgaggg aaaagagaaa
     7081 gttaacgagt atgtggctat cacatcagaa gacaaaaact acttagcaaa attaaaggag
     7141 tccggcttca aattaattat gcaggctact ccaactggaa aagcgaagat ttattagaag
     7201 gtggggaata agaatgattg aatgcatttt aattggcata gttgggtatt taacgacaat
     7261 agatgaaaga tattttgggg catctatgat gaatcgaccg ataatagttg gacctgttgt
     7321 tggacttatc ttaggggatc tccatcaagg catcttgata ggagcagcat tagaagcaat
     7381 gtttattggc attgtaacga ttggtgctgc actaccacca gatgtaggtg tagctggtac
     7441 tattgcaacg gctctagcaa taaaaagtgg agcaggtgct gatgtagctg ttacattggc
     7501 gatgcctttc gctgtattgg cacaaggatt aaacatgttg gcttttacga ttaactcctt
     7561 taccgtttca caaggtagaa aagcaattga aaaccgcaaa ttatcatctt ttcagaagtg
     7621 gcattttttt ccattaatcg ttcgcttacc ttccgcagta cttacatttt tagtagtata
     7681 ttttggtgcc aatgctacag aaagttttgt aaatattatg ccagaaaaat taatgaccgg
     7741 tttcaccgta gcttctggtt tactccccgc tgttggattt gccatgctaa ttcaaggaat
     7801 gattgacaaa aaatcagttc cttatttttt cattggatac cttttagcgg catatttagg
     7861 agtccccgta gttggtgtag ctgttgctgc aattatggtt gctttattag ttatccaaac
     7921 taaacgcaat cctgtaaata tttaaaggga gagtgataat tatgggagat tctaaattaa
     7981 ctaaaaaaga aataaacaag gttttctggc gttcatttgc tttacaggga tccgagtcat
     8041 acactgatat gcaaggaatg ggatatagtt tttctatatt acctgcttta aacaaaatat
     8101 atcaagggaa tgatgaaaaa tattatgaag ctgtttaccg aaatacggaa ttttttaata
     8161 caacgcctca tatttcaacc tttattatgg gtatgtcatt agcacttgaa gaagaaaatg
     8221 caaaaacaga tcaatttgaa actgaaacaa tttcatcgat aaaagctgca ttgatggggc
     8281 ctgtagctgg tattggtgat agtttttact ggggaacatt tcgcgttatt gctgctggca
     8341 tcggcttatc tttagctgcc caaggaaaca ttttaggtcc aatattgtat ttttcaattt
     8401 atacgctgtt acattttctt ggcagatatg gctgcttacg tattggatat cggatgggag
     8461 caaacgccat tgaaaaagca tttgagaaca attaccttga aagtttctcg ttagcagcat
     8521 ctatcgtagg tttaatgtca attggagcca tgacctcatc tctggtacgc tttactattc
     8581 ccgccgtatt taaatcaaaa ggtggagaaa taaatgttca gcaaatgctc gatgatattt
     8641 ttccaggaat tctctcattg gggctaacat tctttgttta ctttctatta aagaaaaaag
     8701 taaaggttct ttggattata cttgctatct tcgtctttag tattgttgga gtttatattg
     8761 gattattagg ttaattaatc cagaataaaa ctagatcaga catcaactta aaccataaaa
     8821 tacccaaaga taaatgtaaa tatgagtaaa aagagcatgg tataaattat taggatctat
     8881 aatttatggg tctgatgaat cagaattgat tctgtagtga cctaaaaaag atagatgttt
     8941 tcggagtgga gaaaattcac tccgtttttc tttgcttttc ttgatgattt tagacagctt
     9001 tgaatgctat tactctaaat tgcaccagac tctgccagtt tagtttcctt ttcatacgct
     9061 catggttgta gtaatgaata tagccatcaa tggcttgctt caattcttca aagatacgat
     9121 agtctcttcc ataataaatt tcctgcttta atagactaag gaagttctca atgatcgaat
     9181 tatctaagca ggtctcctta cgggacatac tttggaagat cttatgttct ttcaacttat
     9241 gactataagc tttcatttgg taggtccatc ctttttggag tgaaaagttc gacgatagat
     9301 acattccgtt gcgcagatag cttcctctaa agcttccatg atagccaacg cacttggctt
     9361 ttcagaaatg cgataaaata aaatttcact gttgtacata tctaaaaatg gatcaagata
     9421 cagcttcttt tcacgattgg caccgtattt atccatttca aaatatttta gttctgtcgt
     9481 atctgttgta accttttgat gacagttgct tgtgtaaaag cgtcgatgaa ttcgattttt
     9541 tgtaatcatg ccgattcttc cacgataagt attatagcgg cgatattttc tcgtaaatga
     9601 tcgtacttca atactcagct ttcgaatcaa acgttgtact tttttcttgt taacatggaa
     9661 cccacggtcc cacaattcct ttgtcatacg taaatagcca taatctttat attgacttct
     9721 tattttaagc atttcctctt cgatttcttg atctgaattt cttcgatcaa atcgtctttg
     9781 ccaatacata aaggttaatt caggaaattt caaggtttca agaagctcaa ctaatttgaa
     9841 tgatcctcgg aggttgtcga tgattcgtgc gattgattcg ttcgtctttg ttagacttcc
     9901 tgttttctaa gcttcctcaa ttcttttaaa aaggtattct caatctgaag gaaacgcact
     9961 tatttttcca attctttgat acgattatgc tctttaggcg tcagtttgct tgtttcttcc
    10021 ttttattctt tcttagacaa aatggaggag gaccctttca tttttgagag cccgtgcaat
    10081 ccttttgaac aaaaattttc atccaattga caactaacgc aggttggttt atccctaaat
    10141 gattcgccac ttcacgatag gatagctcac ccgtttttta taactctatc gcatctatct
    10201 tgaattgaac agaataactt cgcttatttc gcttacgcaa gaggccttct tctccaaatt
    10261 ctcgataagc atttgtagcg accccaaaaa gttagacttt tatggagtgg agaaatccac
    10321 tccttttttg tacgataaaa ttaactgtta gacagctgtt tttacagctt ctcgaaactg
    10381 aaccggactt cgccaattta atttcttttt gatgcgttta tggttgtaat aatagatata
    10441 ttggtcaatt tttgttttca attcatctaa actccgatag acttctccat gaaaaatctc
    10501 ttgctttaat aaaccaaaga agttttccat aggtgagtta tctaaacaat tccctttacg
    10561 agacatactc tggaagattt tatgctgctt caatttattc ttataagcat tcatttgata
    10621 ggcccatcct tgatctgaat ggaaggttct acgaaatgga caatcatttg tcatctggat
    10681 tgcttcttct aatccttcca ttatggctag tgcgtttggt ttttcagaaa tccgataaga
    10741 tagaatttca ctattgtaga aatccatgaa aggatctaaa tacagctttt tctgacgaac
    10801 gacacctgtg gtgtctactt cataatactt aaactctgtg gtatcagttg taattttttg
    10861 gtgacagata tttgtataaa accgacgatg aatacgattt ttggcgacag tgccaacctt
    10921 cccatgataa gaattgtacc gtcgagattt tcgagtgtaa gactttactt cgatacctag
    10981 tttttttatc agacgttgaa tcttcttttt attgacatga atcccttgat tctctaattc
    11041 tttttttagt cgcagacagc catagtcttt atgctccttg cgaatttcaa gcattttttc
    11101 ttcaatttct tggtctgggt ttggtctgtc aaaccgtttc tgccaataca tataggttgc
    11161 tttaggaaat ttcaatgttt caagaagctc aactagtttg aatgatcctc ggaggctggc
    11221 gatgattcgt gcgattgatt cttgcgtctt tgctgggctt cctgttttcg cagcttcctc
    11281 aactctttta aaaaggcatt ctcaatttga agggatcgga cttgcttttc taatgccttg
    11341 atacgctcgc gttcttcagt tgttgtattt tcttttttgt tttctttttt tctagtcaaa
    11401 gtaagtggtc gccctttctc ttttgagagc ccctcaatac ctgcttctct gaaattacgc
    11461 atccaattgg caataagcgc agggttgttc atttctaaag tgctggcaac ttctcgatag
    11521 gacatctcac ttgtttgata taactctagc gcatttaact taaattgaac agaatatttc
    11581 tgattttgat gctttttcaa tagaccttcc tctccgtatt cacgataggc attcacccaa
    11641 tcacttactt gagacttgct tgaaataccg tatttgttgg caagatgccg atattcgccc
    11701 acgccactaa gatattcttt aacgattttt agttttaatt caaaaggata ttttgccata
    11761 caaataacga cccccaaaag ttagattttt tggtctaact tttgattgat ccatccgtgt
    11821 accttttttt gatctcactc cttgtatttt tgctaaatat cctaatccac cttctccttc
    11881 aagatactct tgaacgattg ttgacttgaa ttcaaagcta tattttccca taaaaatacc
    11941 atcccccctg aagttagatt ttggtctaac tttttagggt ttggacatca ccagtcccat
    12001 tttcattcac gtaatgcaag aaactgactg aagtcattaa acaaaaatct gagctagaat
    12061 gaagttcatt ctgactcaga tttgattttg cacaactcat cagtcttcgt aaaatatatt
    12121 ttgcctaata agtttctttt a
//
LOCUS       DACDRN010000069        12140 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDRN010000069 DACDRN010000000
VERSION     DACDRN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.069"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(91..1650)
                     /locus_tag="IWJ80_002234"
     CDS             complement(91..1650)
                     /locus_tag="IWJ80_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP9461931.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1805..4039
                     /gene="pcrA"
                     /locus_tag="IWJ80_002235"
     CDS             1805..4039
                     /gene="pcrA"
                     /locus_tag="IWJ80_002235"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP9461932.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMISYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4056..6092
                     /gene="ligA"
                     /locus_tag="IWJ80_002236"
     CDS             4056..6092
                     /gene="ligA"
                     /locus_tag="IWJ80_002236"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP9461933.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6198..6503
                     /gene="gatC"
                     /locus_tag="IWJ80_002237"
     CDS             6198..6503
                     /gene="gatC"
                     /locus_tag="IWJ80_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP9461934.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6503..7975
                     /gene="gatA"
                     /locus_tag="IWJ80_002238"
     CDS             6503..7975
                     /gene="gatA"
                     /locus_tag="IWJ80_002238"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP9461935.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7972..9402
                     /gene="gatB"
                     /locus_tag="IWJ80_002239"
     CDS             7972..9402
                     /gene="gatB"
                     /locus_tag="IWJ80_002239"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP9461936.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9412..10467
                     /locus_tag="IWJ80_002240"
     CDS             9412..10467
                     /locus_tag="IWJ80_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9461937.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            10578..11951
                     /gene="rlmD"
                     /locus_tag="IWJ80_002241"
     CDS             10578..11951
                     /gene="rlmD"
                     /locus_tag="IWJ80_002241"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9461938.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            11954..12082
                     /locus_tag="IWJ80_002242"
     CDS             11954..12082
                     /locus_tag="IWJ80_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9461939.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         4198 a         2237 c         2629 g         3076 t
ORIGIN      
        1 gtttttccca gtctcttctt tttttataga atcatataga atcaagtagt gtagatgaat
       61 agttgttgta aaatagtttt tccatgttcg ttaaaatgat tgcttcaaat ttgtgataga
      121 agatttagcc tcgatttcct gcttgattct tggcaagttt gcttcatcag tcaagataac
      181 caaaatatct cctacattca tgatcgtatc accgtgagtc aagatttcct gatttcctct
      241 gcgtatagag attagcagca tatttttagg ccaattgaag tctcgtacca ttaatccgtc
      301 taaagaactc tctgccgtta ctggaaaatc aaaagttgtc cttgcacctt ttagtgaggc
      361 ggtctgagta tttccaagta gacgttccaa taatgattcg taaattggat taccgccgag
      421 aaggtcattg acgatataag caacaagagc agccaatcct aaaggcatca gatgattaag
      481 agagccaacc atttctgtca ctaaaataat tgctgtcagc ggtgcttttc caatcgctgt
      541 gaaataacca gccatggcaa taacgacaaa atccttgatg tagattggat ccatcccgaa
      601 atcaaccata atgtttccat aaaagctacc gattaaagcc cccaatgtca atatcggaag
      661 gaaaatcccc cctggaagac caccaccgta agaaatcatg gaaaaaacga atcgaagaac
      721 aaacaaaccg ataatcataa tgattggcaa ggaagcatgt ccaaaagcta aaacgatttg
      781 attgcctccg cctagagtac taggccataa ataaccaatt ggcaaaatca gcaagaaagg
      841 aacgaacccg tgaaaataag ctggtaaagg caatcggttg taaaattttg gcaaagccag
      901 caagaccttc tgataaataa aacctaagat gcctagaagt actcctaaga tcactaagga
      961 accataatat ttcaacggca aagaaggaag gtcacctaga tataaaacag gtctcaagcc
     1021 gaaaaagtaa agagagacga agttagcagt gattgctgaa gcaaatgatg tcagccaaac
     1081 aagtggagaa aaggagtgat ggatttcttc taaaacaaag agtaatcctg cgataggagc
     1141 attgaatgct gctgcaagtc cgctacttgc accgctagaa attaaaatct tttcttctga
     1201 agatggactc cgaaacaatt gtccagctcc ttgtccaatg gatgcgccaa gctgtataga
     1261 aggtccttct ctacctagaa aaaggcccgc accaactgaa agtacaccgc caatgaactt
     1321 tttccataaa acagagaacc agttcattga taattccccg cgaagctgtc cctctacttg
     1381 tggaatcccg cttcccttga tatccggttc atttttgacc agataaccca cgaaaaatgc
     1441 tgcaaggatg cagcaaatag ctaggccaat taagtaaatc ggctgttccc gactcatttc
     1501 atagatactt cgaactaaat cgcttagctt ttcgatagat aatcgaaaca ggctaacgac
     1561 gattcctgtc agcagtccaa caaatattcc ttttagaata aaatatatct gagtcttatc
     1621 taatttcttg atttttcctt ttttttccat tcaaacgcct cgctttcttt taatttagcg
     1681 cgaaatcaac gaattgacaa ggaaaaatcc ataaaaagaa acaaatgatt ttgaactagc
     1741 tagtttaatg aaagccaaga aaatgctata attaacaaga ttgataaaag aaaggatgtt
     1801 gttaatgtca tccaaagctg aattattaaa cgggatgaac ccccgtcaga aagaagctgt
     1861 tcttcacact gacggaccat tactgctaat ggcaggtgca ggaagtggaa agacgagagt
     1921 gctgacacac cgcattgcct atttgattga ggaaaaagaa gtcaatcctt ggaacatcct
     1981 agcgattact tttaccaata aggctgcaaa agaaatgaaa gaacgtgtca atgcgattct
     2041 cgcatctgga ggcgaagatg tctgggtatc gacttttcac tcgatgtgtg tgcgtatttt
     2101 aagaagagat gttgatttta tcgggtataa ccggaacttt acaatcatcg attcttctga
     2161 acagctgacc ttgatgaaac ggatactaaa agaactgaac attgatccga aaaaatatga
     2221 tcctcgaagt atattaggaa caatctctca agcaaaaaac agcctgcaga cccctcagga
     2281 ttttgcaaaa atgcaaggca gttattacga agagattgcg gcaaaatgtt atgctgctta
     2341 ccaaaaagag cttcaatata atcaatgtat ggactttgac gacctgatca tgaatacgat
     2401 tcgattattt gaagaacatc ctgattcact cacatattat caaaacaaat tccattatat
     2461 tcatgtagat gagtaccaag atacaaatca cgcacagtat acgctagtca atttattggc
     2521 aggtagattt aggaacctgt gcgtcgtggg agatgcagat caaagtattt acggctggcg
     2581 cggtgcagat atgcagaaca tcttggattt tgaaaaagat tatccagatg ctgcagtaat
     2641 tttgctagaa caaaactatc gctccacgaa aaacatcctt agcgcagcaa accaagtcat
     2701 tgaaaacaat agcaatcgaa aaccaaaaaa cttgtggaca gaaaacaaag aaggaaacaa
     2761 gatcacttac taccgtgcag ataatgaacg agacgaaacc cgttttatcg tagatcggat
     2821 gcaagaagag attcgaagca atcatcggaa ttacggcgac tttgccatct tgtaccggac
     2881 gaacgcacaa tctcgtgtaa tggaagaaac tctgttgaaa gcaaatattc cttataaaat
     2941 ggttggaggg cacaagttct acgatcgtaa agaaatcaaa gatattttag cgtatttgaa
     3001 tgtgctggca aatcctcaag attcaatcag ttttgagcgt atcgttaatt cgcccaaacg
     3061 aggcatcgga ccaggatcta tcgaaaaact acgcagtttt gcttctcttc atgaatggcc
     3121 actgcttgaa gcagcgcaaa atgtggatct ggcaaatatc ggtggaaaag caggacagca
     3181 attaggtgca tttggagaga tgatccaaga agtcactcag atgatctcat acttgacagt
     3241 gacagaattg acgaaagagg ttttggatcg tagcggatat ctagaagact taaaaatcca
     3301 aaatacatta gaagcacaag cacgcatcga aaacttggag gaatttttaa cggtcacaca
     3361 agaattcgac aaacaattcg aacagcaaaa tgaagaagac gctgatgcac cagaagaaaa
     3421 actaacggtc ttcttaaatg atctggcact tgtttccgat attgacaatt tagaagaaga
     3481 tgcttcccaa gtgacattga tgactttgca tgcagccaaa ggattagaat tcccagttgt
     3541 attcctgatt ggtctagaag agggggtttt tcctttatct cgagcattga tggaagaaag
     3601 cgaactggaa gaagaacgcc gtctcgcata tgtggggatc acgcgagcag aagaagcgtt
     3661 gtatttaaca aatgctttct ccagaacatt atatggacgg acgcaatata accgaccttc
     3721 tcgttttgta gaagaaatcg atcaggagtt actggaaatc gaaggcatgc gtcctacgcc
     3781 taaaaaaaca cctgtattcg ctaaaaaaac agcatatagc tataaacagc cagagacagc
     3841 ggttgttccc tctaaaagtg ctactggagg ggaaaataac agctggaaac ctggagataa
     3901 agtcaaacac aaaaaatggg gtctaggaac agtcgtccga gtaagcggga cgtcgaagga
     3961 tctagaacta gatgtggctt tcccaagtca aggagtaaaa cgtcttttgg ctgcctttgc
     4021 gccaattgaa aaagcttagt agtggagggt aaatgatgga acatgaaatg acgattgacg
     4081 aagctgctaa acgagcagaa gaacttcgta caagactgaa ccaatggtct cgcgaatact
     4141 acgtagaaga taaaccaaca gtggaagact atgtttatga taaagaatac gcagaattag
     4201 tagcaattga ggaacaatat cctgatttga tcacgtctga ttctcccact caacgagtag
     4261 gcggaaaagt attagaaggt tttgaaaaag tcactcatga tattccgctt tacagtttga
     4321 acgacgtttt cagcaaagaa gagctgatag cttttgatca acgtgtacaa aaagcagtgg
     4381 gacgtgtagt tgactattgc tgtgaactaa aaatagatgg actttccgtt tctttgcgtt
     4441 atgaagatgg aaatttcgtg agaggtgcga ctcgcgggga tggaactgta ggagaaaaca
     4501 tcactgaaaa cctaaaaact gtccggtcgg tcccaatcaa attaaaagaa ccaatgaaca
     4561 tcgaagtaag aggcgaatgt ttcatgccaa aacggtcatt tgtccaattg aaccaagaca
     4621 gagaagcaga gggaaaagat atctttgcga atcctcggaa cgccgcagct ggttctttgc
     4681 gtcagttaga ttctaaaatc acagcgaaaa gaaatctgga cactttttta tacacagtag
     4741 ctgatttcgg tcctatgcaa gcaaaaaccc agtatgatgc gctagaagag ctagaaaaaa
     4801 ttggattcca cacgaatcgg gaaaaacgtc tatgtcattc gattgatgaa gtgtggtcat
     4861 acattgaaga atatcatgac aaacgtgtgg atttgcccta tgaaatcgat ggaatcgtca
     4921 tcaaagtgaa tgaattctcg ctacaagatc aacttggttt tactgtcaaa gcaccgagat
     4981 gggcagcagc ttacaagttt ccgccagaag aagttgaaac gctaatcgaa aacatcgaat
     5041 ggacagtcgg tcgaacaggg gtagtaacgc caacagctat catgacacca gtccgagtag
     5101 caggaacgac agtaagccgt gccagcctgc ataacggtga ttatattaag ttgaaggata
     5161 tccgcttgaa agatacagtt ctgatttata aagcgggaga catcatccca gaagtttcgc
     5221 aagtcgtgtt agataaacga cctaaagaca gcgaagaata ccaattgcct acacattgtc
     5281 cagtttgtgg aagtgaactt gtccatttgg atgaagaagt agccttacgg tgcatcaatc
     5341 ctaaatgccc tgctcaaatg aaagaaggtt tgaaccactt tgtttcaaga aatgcgatga
     5401 atatcgatgg gttggggccg cgcgtactag aacagatgta tgataaaaaa ctagtagcag
     5461 atgttgctga tctttacaaa ttgacagaag aagagttatt gacgttggac aaaataaaag
     5521 aaaaatcagc aaataatatt ttgacagcaa tcgacaacag caaagataat tcggtcgagc
     5581 ggttgatttt cgggttagga atccgacatg tcggagcgaa agcagcaaaa attttagcag
     5641 aacatttcgg tgatctagaa acattgagta aaagtgacta tgagtcgatt attgcgcttg
     5701 atacgatcgg tgatatcatt gccgacagtg tagtcactta tttcagtaac gaagaagtcc
     5761 atgaattgat gaatgaatta aaacaagcag gcgtcaattt cgaatacaaa gggcttcgaa
     5821 atgcccaatt gcaagaagtc gagtctcctt ttaaagaaaa aacagtagtc cttactggta
     5881 aactaacacg atttactcgt gaagaagcaa aagagacgat cgaaaatctt ggcgggaaag
     5941 tgactggtag cgtatcgaaa aaaacggata tcgtggtagc aggagaagat gctggcagca
     6001 agctgaccaa agcacaagaa ttaggaatcg aagtttggac agaagatcaa atggcagatg
     6061 cattggctaa aagtcgctca gtagaagaat gaaaaatgag ttttttcaga caaatttcaa
     6121 taaacaattt cagacacggc actcttgtgt taaaatagag agcaaagttt ttaactcgtt
     6181 tagaaagaag gggtagtatg gcgattagtg aagaacaagt gaaacacgtt gccaaattag
     6241 caaaacttgc gttcgcagat gaagaacttg caagttttac cgaccaatta ggtaaaatca
     6301 tcgatatggt tgaaatgctg gaagaagtag atacagaagg tgttccattt acgtcgaatg
     6361 tggttgaaac agtaaacgtg atgcgacaag atcgggcgca agctggatgg aaccgcgatg
     6421 aattattgaa aaatgtgccg gaaacagaag acggctttat caaagtaccc gcaatcatcg
     6481 ataatgggga ggctggcgca taatgacaga attacacaat cgttctctag aagaactgca
     6541 tcatatgctt gtatcgaaag aaatcactgt acaagatttg acaaaagcaa catttgaacg
     6601 aatcaaagaa acagagcctg aggtcgaagc attcattaca ttgaacgaag aaaaagcatt
     6661 ggaacaggca aaagctttag atgaaaaagg gattgatgaa agcaacgtcc ttgctggtat
     6721 tccaattggt atcaaggata atatcgtaac aaaagatatc ttgacaaccg ctgcatctaa
     6781 gatcctttac aattttgaac caatttatga tgcaacggtc atggataaaa tctatcaagc
     6841 agatatgatc cctgttggta agctaaacat ggatgaattt gctatgggtg gaagtacaga
     6901 aacttcttat ttcaagaaaa caaaaaatgc ttgggaccca acaaaagtac ctggtggctc
     6961 ttctggtggt tctgcagcag cagttgcagc tggacaaatt ccagtttctt taggtagtga
     7021 tactggggga agtatccgcc aaccagcagc ttttaacggc atcgtaggga tgaaacctac
     7081 ttatggccgt gtctcacgtt ttggtttgat tgcctttgct tcatctcttg accaaatcgg
     7141 accaatgacc cgtacggtga aagataatgc attagcattg acagctatca gcggttatga
     7201 cgaaaaagac ggtacttctt caggcgtttc agttcctgat tttgcagagg gactgacagg
     7261 cgacatcaaa ggaatgaaga ttgctttacc taaagaatac ttaggcgaag gtgtggcccc
     7321 tggcgtaaaa gaagctgtat tgaaagcagc agaaacattc cgttcattag gtgctgttgt
     7381 ggaagaagtc agcttgcctc attcaaaata cggtgtggct gtttattata tcattgcctc
     7441 atcagaagca agttccaact tgcaacgatt cgatggtatc cgctacggtt atcgctcaga
     7501 aaatgtgaaa tcattagatg atgtatatgt caactcacgt tcagaaggat tcggtgaaga
     7561 agtaaaacgc cgtatcatgt taggtacatt ctccttaagt gctggttatt atgacgctta
     7621 cttcaaaaaa gcgggacaag tccgtacatt gatcaaacga gattttgaaa aagtctttgc
     7681 agattatgac ttgatcatcg ggccatcttc accaacagtt gcattcggaa tcggtgagaa
     7741 cattaacgat ccaatcacaa tgtacatgag tgacatctta acgatccctg tcaacttggc
     7801 tggacttcca ggtatgtctg taccggcagg tttatcagaa ggattaccag ttggattgca
     7861 gatcatcggt aattattttg atgaaaaaac gatgtatcaa gcggcatatg cgtttgaaca
     7921 agcaacagaa ttccataaac aacaaccagc tattttagga gggaaagacg aatgaatttt
     7981 gaaacagtca ttggactaga agtccacgtc gagttaaaaa ccaactcaaa aattttctct
     8041 ccagcgccag ctcattttgg tgcagaacca aacagcaata caaacgtgat cgactggggt
     8101 tatccaggcg tgctgccagt aatgaataaa gctgcgctgg aattcgggat gaaagcagct
     8161 ttggcattaa actgtgaaat ctctaaagat actcattttg accggaaaaa ttacttttac
     8221 ccagataatc caaaagcata tcaaatttcc caatttgatc aaccaatcgg tcatgacgga
     8281 tggatcgaaa tcgaagtaga aggtaaaaag aaaaaaatcc ggatcgaacg tgtccattta
     8341 gaagaagatg ctggaaaaaa catgcatggt attggcggtt attcttacgt tgatttgaac
     8401 cgacaaggca cgcctttgat cgaaatcgtt tctgaagctg atatgcgttc accagaagaa
     8461 gcatacgctt atctagaagc attgcgatca attatccaat ttacagaagt cagcgatgtg
     8521 aaaatggaag aaggctctat gcgctgtgat gccaatattt ctcttcgccc ttatggacaa
     8581 gaagagttcg gtacgaaagc agaattgaaa aacttgaact ctttatcttt cgtgaaaaaa
     8641 ggtttagctt ttgaagaaaa acgtcaagca aaagttcttc tttcaggagg agaaatccaa
     8701 caagaaacac gccgttttga cgaaacaacg aataaaacag ttttgatgcg tgtcaaagaa
     8761 gggtctagtg attatcgtta cttcccagag cctgatatcc cgaaattcgt gatcgatgac
     8821 gaatggattc aaaaagtaaa agctagtttg cctgagatgc cagcttctcg tcgcgaacgc
     8881 tatatccgtg agtttggact tccagagtat gacgcaatgg tattgacact tacaaaagaa
     8941 atgtctgatt tttttgaagc tgttttggaa aatggtgcag atgccaagca agcatctaac
     9001 tggttgatgg gtgaagtttc tgcttacttg aacagtgaga aactggaatt agctgagaca
     9061 aaattgacac ctgagaactt agcaggaatg attcgtttga tcgccgatgg aacgatcagc
     9121 tcaaaaatcg ccaaaaaagt cttccgtgaa ttgatcacaa atggcggaga agcccaagaa
     9181 gtagttgaaa aaaatggttg ggtgcaatta tctgatccga acaaactact gccaatcatt
     9241 aatgaaattt tggataacaa ccaacaatct gtcgatgatt tcaaaaatgg aaaagatcgt
     9301 gcggttggct tcctagtcgg tcaaatcatg aaagcaacta aaggacaagc aaatcctggt
     9361 gtcgtgaata agctacttaa agatgaatta gcgaaacgat agaggctgaa aatgaaaaaa
     9421 gcacgcgtaa tttataaccc cacctcggga aaagaattat tgaaaaaaaa tttggcggat
     9481 atcctacagg cattagaaga agcaggattt gaagccagcg cctatgcaac tacaccagaa
     9541 ccagattcag ccaagaatga agcatgtaga gtcgcagaac taggttttga tctagtagtg
     9601 gcagctggtg gcgatggcac aatcaacgaa gtggttaatg gaatcgctcc gcttgaacac
     9661 cgtcctaaaa tggccatcat cccagctggt acaacgaacg actatgctcg agcattaaaa
     9721 attcctagag ataatattaa agcagcagca gaagtgataa aaaaacacca aaccgtaaaa
     9781 atggacatcg gcctttctga taacagttac ttcatcaata tcgctgcagg tggctatttg
     9841 acagaattga cttatgaagt tccatcagaa ctaaagagta tttttggtta tcttgcttat
     9901 cttgcgaaag gagcagagat gctccctcga gtaaagccaa tcaaaatgcg cttgaagtat
     9961 gatgaagggg aatatatcgg aaatgcttcg atgtttttcc tagggctgac gaactctgtt
    10021 ggaggattcg aacagatcgc gccagatgcc aaattggatg atgggaagtt ttctttaatc
    10081 attgtgaaga cagcaaatat ctttgagatc ttgcatattg cagcactcat gctgaatggc
    10141 ggtcgacatg tcgatgatcc aagaataatc tataccaaaa caagcagttt gttcgttgaa
    10201 acagaagatg atatcaatcg accagtaatg atcaacttag atggtgaata cggcggggaa
    10261 gcaccgatgc atttccaaaa tcttcatcaa cacattgagt tctttgccaa taccgatcag
    10321 attcctgatg aagcaatcac aggtacagat gaagaagaat tagaggttgt cagcaaagaa
    10381 ttcgtaaaag aagtcgaacg tttaacggat gaagatatcg acggtgacgg aaaaatagca
    10441 gacgaaacac ctaatcaaaa gaattaatca aaagccggtg ctgaacgtag taaggtcagt
    10501 gccggtttta gttgtgtaat tagctgattt atagtagagt tcagtaggat tgaaaaatag
    10561 gcttaaagga gcaaaatatg acagaacagc aaatagtaaa gaaaaacgaa cgatatacag
    10621 tcgaaatcgt cgacttgagt tatgaaggaa tgggggtagc aaaaatcgat ggttatccat
    10681 tatttatcga aaatgcactc cctggagaag aagtagaaat actagtagta aaagcaggca
    10741 ataagtttgg atatggaaaa gtcgaaacat ttattacaca atcacctgat cgtcagcctg
    10801 tagaaaacaa tgcattactt cgcataggta tcgcaccttt agcccatttg aagtatgaac
    10861 aacagcttgt cttcaaacaa aaacaagtag agcatgtaat ggcgaagatt gctaagatgc
    10921 cagaagttcc tgtacttcca acggtcggaa tggcagaacc attcggctac cgtaataaag
    10981 cacagatccc tgttcgccgt atcgatggac agttagccac cggtttttac aaaaagaata
    11041 gccatgatct agtccctatc gaagattttt atatccaaga tcctgtcatc gatcaagcaa
    11101 tcaaagtagt cagagatatc ttgcaacggt tccaagtacg aggatataat gaagaaaaaa
    11161 atgaaggaca gcttcgtcac atcattatcc gtcgtggtca ttattcccat gaaatgatgg
    11221 tcgttttagt tactcgtaaa gaaaaattct ttaaatccaa ggaaattgct gaagctattc
    11281 atgaagaatt gccagaagtg gtttcggtca tccaaaatat caatgaagaa aaaacaaatg
    11341 tgattttagg agacaaagaa aaagttttat atggcagaga ctatatcgaa gatcagctat
    11401 taggaaaaac ctatcgaatc tcagcaaagt cattttatca agtaaatact caacaagcag
    11461 agtatctgta ccaaacagcc atcgagtttg ccgagttaga taaaaaagac attgtgatcg
    11521 acgcttattc aggaataggt acgattggct tgtctttagc agacagagtg gataaggtgt
    11581 atgggatgga agttattcca gaagcaattg aagatgcaca gttcaatgct ttaaataata
    11641 aaatcgaaaa tgttcattat gaagtcggaa aagcagaaaa agttatgaaa aaatggcaag
    11701 aaaaagggat caaaccatct gtgatcgtag ttgaccctcc acgaaaagga ttagatcatc
    11761 attttattga atcagcaatc gaaatgtcac cagaacgaat cgtctatatc tcatgtaatc
    11821 cggcaacttt tgcaagagat gctaagttat ttgcagaagc aggttatgcc gtgaagaaag
    11881 ttcagcctgt agatcttttc ccgcaaaccc atcatatcga attagtcgct tcattcgtta
    11941 aaggagaata aaaatggacc aaaaagaaaa aatgaaagaa ttgatcaaaa agaaacaagg
    12001 tggaggccga tccaaacaaa tggaaacacc taagaatgat cgtaaaaaca tgagaaaagg
    12061 ccctaaaatc tttaataaat agacgtgcta aagaaagact aagaagtgtt aacttttgtt
    12121 attgtttctt agtcttttta
//
LOCUS       DACDRN010000070        12032 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDRN010000070 DACDRN010000000
VERSION     DACDRN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.070"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(602..675)
                     /locus_tag="IWJ80_002243"
     tRNA            complement(602..675)
                     /locus_tag="IWJ80_002243"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(639..641),aa:Ile,seq:cat)
     gene            complement(865..1890)
                     /locus_tag="IWJ80_002244"
     CDS             complement(865..1890)
                     /locus_tag="IWJ80_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9461940.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            complement(1887..2111)
                     /locus_tag="IWJ80_002245"
     CDS             complement(1887..2111)
                     /locus_tag="IWJ80_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9461941.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(2108..2401)
                     /locus_tag="IWJ80_002246"
     CDS             complement(2108..2401)
                     /locus_tag="IWJ80_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461942.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            complement(2439..2576)
                     /locus_tag="IWJ80_002247"
     CDS             complement(2439..2576)
                     /locus_tag="IWJ80_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9461943.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            complement(2578..3024)
                     /locus_tag="IWJ80_002248"
     CDS             complement(2578..3024)
                     /locus_tag="IWJ80_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461944.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(3187..5313)
                     /locus_tag="IWJ80_002249"
     CDS             complement(3187..5313)
                     /locus_tag="IWJ80_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461945.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            complement(5337..7628)
                     /locus_tag="IWJ80_002250"
     CDS             complement(5337..7628)
                     /locus_tag="IWJ80_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461946.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            complement(7638..8375)
                     /locus_tag="IWJ80_002251"
     CDS             complement(7638..8375)
                     /locus_tag="IWJ80_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9461947.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            complement(8426..11857)
                     /locus_tag="IWJ80_002252"
     CDS             complement(8426..11857)
                     /locus_tag="IWJ80_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP9461948.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIDNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
BASE COUNT         3477 a         2523 c         1894 g         4138 t
ORIGIN      
        1 gactgacgtg ccggacgcac gtagcgatca tcggcgttat gactatataa agtatacttt
       61 cgttgatcca gaaaggtata aagattaaag taggctacct aaaaaggtag cccggaacga
      121 attttatcac catacttgtg aaaggagata ttttttaagt tagtattaag attgtgtaat
      181 atgatgatat ctatatttta tagtatcagt gctataaaat caaaaataag tcactaatta
      241 actaccactc caattgtaag ccttttttct cacttttttt caaaaatatg gtatgctttt
      301 taatggcttc aaatataaaa gagtttaaag cgtaacacac ttatggggaa gtggtttggg
      361 gtgcgcttta aactcttctt tattattatc tcacaattta acccaaatgt ctttctattt
      421 aaaaatcaaa gtaaaacttt tcaaatatac agaagtataa ctatgtgaaa catcctttca
      481 ttaatccata aaaggataca taaaaaagcc actcatttga gtggcaatga agaaaagctt
      541 tagcttgtat aatactcttc aaaaaaattc taacacagaa cgattcaaat ggctacgtta
      601 atgtaccctg taggactcga acctacgacc ggacggttat gagccgtctg ctctgaccaa
      661 ctgagctaag ggtactggtt gttgccacat aaagccataa acaatcaacc agtagaatgt
      721 gtggcaacaa acctgttatc gcatagcttg gagtgtgact atttatgggt gatagtgaag
      781 atatgcgata acatcactat tttatcgaat aatttttata gttgtcaata tagttatgta
      841 ctgctcctca acgaggagct atttttatcg tttaggaata tttaaatacc aacgtttgtc
      901 atggaaatct tgcgcaccgc ctttagtgtt tccctctgga tcattcgttg cccgcatcat
      961 tacatagact ttcttactag gaaaattacg catattgaaa gatacatgat aaccaacatt
     1021 tccagaagta ttataagctt ggtttacatc tggtctataa attccatcag ctcttactcg
     1081 agctaattct ttcccagtat tgtagtccat aatgaaaata tactcgtatt tatagttagc
     1141 aatgtgccat ccagccacat gcaagtttgc gttttcgatt tccccaaact gatcaatgtg
     1201 ggcgtgattt gttccatctg tcagcgtagg attagctgca ccagctcgtg ttggatcaat
     1261 gacaggcttg ttttcagaag ttgttggatt ttcatcggta aatccatgag ctaaatcata
     1321 agcaagcttt tctttgctaa ctcccatttg cgataagtaa ccatatggat ctgtgtggtt
     1381 cccccaaaca taatttgtca cccacaaatg agaaatgatt ccttttgtaa ataaagaagt
     1441 tccttgatca agagtcaatg gaattccata tttttttgca ctatctcttg tatattcaat
     1501 ataagctcga tagttttttt caaacaatgc tttatcatat gtgcgctgta attcaatctg
     1561 tacaggcgca taaggattag cgttaccagc tccccacgaa acatatcctt gctcacccac
     1621 acggtaaaca atcccaccgt caccaataac atctgtagta taagaattgc ttccgttata
     1681 attatttttc atgttggcgg ctacgtttct tgctggtgca tctattccag tttcgtgcaa
     1741 aataattttg ttaggaattg ctaatctgga gtctccttga ttcggcgcta aattatactc
     1801 gtcattaata gtataagcaa acgtattaat gggtaataaa aaaagagccg ttaacaggct
     1861 catcgcagta atagtaattt tctttttcat ttgtttcctc cttcttcgct ttcagccgag
     1921 aacattttgt aggttcgatt tgatacaccc aacacactcc ctaaaaacgc gccaaaacca
     1981 gtaatgatga caacacagat atctgtgtac tgccaattga gcgctttacc aactaacccc
     2041 acgaaagtag ctagtgcggg aataattacc agtgcgaacc attttagtac ttcgaacgtt
     2101 ttattattca ttttcttctc tccctaaata aagttttaat ttgttgcgtg tgttctacca
     2161 atttttctgc atgtgtatct aatctttcat cgtgtttctt tagttcttca tgaatcatca
     2221 atcgatctga tttgctcgat tctaaatctt tagtcagcaa atctaaattg tgacttactt
     2281 ttgaaagagt ctcagtaatc ttcgagaaag atgcagtaat tggttttatt actaataaaa
     2341 tcaaagaaac gatagcggtt attgatcctg ctatcgctcc ccattcccct aaattaatca
     2401 tgtgacaact ccttgaatca aaataaaaag cacatcaatt aagatgcgct ctcttctttg
     2461 ctaatgattt tatctgcttt ttcttcagta atgcacaacg gaacgaaaac cattacttgt
     2521 tcgttagtga aacagcccca atcatacatc attttcacat cgctaaaact aaacatacta
     2581 ctcacctccc tttgaagctg gatttagttg ctctttaatt tctgaaatgt ctttgctatt
     2641 ttgtaacgaa gcaagcatca tttttgaatt gatttgtgct aaactatccg ctttttcttt
     2701 caatgcagta ttttcctgtt taattgctac atcgcttagc atgagtttgg cattgatctg
     2761 ttttaaatcg ccgttctcat tttctaacga ctcatacatt gctttgagat tgtttaaatc
     2821 gttgtgatct agtgcgttcg ctaaaataat ccattgattc aatttaggat caaacatttg
     2881 atcagcgatt gttaacggtt cgccatcagc acgaattcct tcaagcggtg gctgatctgt
     2941 gtaaggaacg gatacaagca tgtcgtccaa tacttttcct gcgtactctc cgccagtacg
     3001 tccatatttc caaatgtttt tcatttattt ccctcccagt aattgaattt cggtttccaa
     3061 tttggaatcg gtggttcgac ttctgtgcat tcttccggta aatgttcttc atcattcaca
     3121 atgatttgct cgaatccgta aggttcaatt ggtctatatg ctgccttcat atcgattcac
     3181 gcttttctaa atcgtgtaag taattgcaaa ggtataatcc gatccataac ttgagtttct
     3241 tcgccattta atggctccat ctgcaccaat agataactga gcactgttca aagtagaacg
     3301 gtctatcgag ccaaccagtt gctcaaaact aattggtggc cgatagcctt ctggaattgt
     3361 tagtatcgtt gaatcatttc caccactgct ttttccgttt aaagccacaa aatatataga
     3421 aactgttttt ccttcacgat aaagctttgc tgatccggta ttcccgtttg taactgttaa
     3481 tgtggtagta gctgtatcat taatgcgttc atcgatctta ttgtctaatt catctatagc
     3541 agtcgcatta gcattcgctt ttgtttgagc atccttagct gtggtgtcta cttcattaat
     3601 tgaagcagtc aactgcgaat taatctccga tactttccca tcggtataat tgtttgcttt
     3661 accagtaatt tcagaaattt taatatctgt ggccaaatta tcttcgacat attctggtgc
     3721 tagatcccaa acataatctt ttggattgtt tgaatcacgc ataccagtac cacgatattt
     3781 atactcacta atattcgggg ttcgagtatt gcctttttct agtttgagcc actcaatttg
     3841 aacagctcct tttgtagctg acgggtattg gtatatttcc agataattag ttaccccact
     3901 atctatatgt tgcttagtta cagtaaaagt taactcccat gtattagcta acccctcagt
     3961 aggcttcatg tctcctactt taacgcggcc agcagcaata tacacggcaa acgattgaac
     4021 tgttagttta gttgctttca ttgatatggt atatgtttgg tcagcgatat agttttctgt
     4081 attgcgtcta ccataaacca tatattcact agttttgtgt ggaaagacaa ctgtagggtc
     4141 agcaatattc tcgcctaaag gaactttacc taaccagtaa gggccatcta gtaagttagg
     4201 gtagtgtgct tttaacttaa cgtcatagct gacactaacc gttcctttat attcactatt
     4261 aagtgagaat atttcaacat aacagtttgt tatgctagtt acattatctt ttgttatacc
     4321 agtaaatgta tgagtgacca ttttatcgcg ttctacattt gctggtatta atgcattaat
     4381 gggcagttct ataccacctt gttcatttaa atatctaagc ctaaccccta tagtattggt
     4441 ctctccagtc caatctgaac cgacactcat agtaacgctt aatgtgtatg tcgtagatgg
     4501 tcgtaaagtg gttataagat acctattatt agcttccgct gtacacggaa tacctaagcc
     4561 aagcgataat atgtcgccgg aattagctgt tctagtttta gtatgagtta taaccctttt
     4621 aacactatct ttaactgtat gtccattacc taacaaaggt ccaacccaag gttctgtaat
     4681 atatggcatt aaattcgggt tctccgaata atcatagtcc ccgaagtcga tgctgttact
     4741 gtacatcact tgtaagttac ctaacttaga aatttcttct ttcagagcat ctaacttgtc
     4801 ttgtagcgtt ttagcttgac cagttaaatc agtaatctgt tgatttaagc tatccactct
     4861 acctttgatt tcagccataa aagcatcaaa agtttcgttg tactttcgaa tcaactcttc
     4921 taattgcgaa acatattcat cggcttggcc ttgcgaaatg tcagacactc ctagtgagaa
     4981 aaaaatgata tcttgcgttg ttaaaatttg attgtctttt ctatattcta cgtagcagtg
     5041 tttataatat cctgcttcac tcataaatgt gccatcaaga gaaaacgtga cttcttcact
     5101 agttacacta gttgcaacat tatctacgta acggttagat ggtgttgttc cttttaaagt
     5161 aaatgttccg ccactcgtat ccatctgcaa gccatttaga aacggtttaa ccgtcaccgt
     5221 aatcccttta tcaccttgac gagccataat agctttggtg tagtttaatt ctttgctgaa
     5281 atctaaagcc aaattatata aactgctagc catttatata cctccttgtc ttcgttttaa
     5341 aaacgttttt ggtcaagcac tgtgctatca tatgctgtat cctcttttaa tctaatatct
     5401 tcatacccta gacggtgtgc cactaaattc catctaacta atacgtttgg cttactagtt
     5461 tcaatgatga aatggtcaat atcttcatga gtaacagcac acaaaactag ttctgtaggt
     5521 gtcacatgtg tcatataccg acttagattt actgtttcag caaacatggg gtcaatatca
     5581 acacgaactt taccattgtc acctgtaacg gcttccccat aatcagcgaa ataatattct
     5641 ggagtttcat aagcgttcaa tagtcgttgt ccataatgtt ctgttggtac agttgagttt
     5701 ttagtacctc taacagtaaa atctttatat acttgtaccg ttgattgttc aaacctagca
     5761 agtttcccat cttcccatga accaaaaaaa caacctggta acgttagcat accatcacta
     5821 gtaaatttca tagtcctacc agctacctta aattcccatg agttacccgc actaccatta
     5881 atgcttaaag aactaccgtc gccagaagtt acataactag cattgctata cctgaaattg
     5941 ggcgcaccaa aagatagaaa cggtctgtta ttaccattat cccacgtact aaaaaccaag
     6001 ttaccctgtg gatttctaat catgaaacca ccaccagttt tcatggtgta tgatacaata
     6061 ccggcatcag cacttacata atcacgtgct tctagctcca taatatcttt gttaactttt
     6121 tttgagtacc aagtcatttt gccattagca atacttgttc tataatcagc accatcacta
     6181 attaatgtag tacctctaat agtaattcct actatttcac cagccgtaat aaacgaggca
     6241 ttgaatccgc catctaacgt ccatgccgtt tcatatgttc cattaatgcc agttttagaa
     6301 aaaccaatac cagcattgtt gatttgtaaa acattccttg cggtattctt atctggtgtg
     6361 tccataatca aaatacgact aggcgcttct ttaggatcta ataaaacata accaccattt
     6421 tgaccagtaa tcatatcagt ttgatgatct acaatatcat tgagtaaatc actgatttcg
     6481 ccaccgtttt tcaattgatc aatggcatca ttaatcaaat tgctgacatt attctctgtg
     6541 ttttctaaga agtttgtttt gacgtttcct acaactaatt tatcgtatga attggttaga
     6601 acattaaacg tatattccac aattctcgct gacatattca cttttaactg tggatgatac
     6661 acatctactc cgtcacccat cgaaactttt tctagatcaa caaatttttc atagcctctt
     6721 tgatgcctca atggtactaa ttcaatcgaa ccactcactt gtggtttttg tttatctatg
     6781 tttgttttca accagtcttt agcagcttcc cttaatgtgg ctacatcagt cgctttgtct
     6841 ttaaaatcaa caaaagaaac atatccagca ggataatcat ccacgtaatc cgtgaaaata
     6901 acttcttctg gtagagtgat ctcgtcttct ccttctgaag agctgctaat gaatggataa
     6961 actccaacta aaacactttg agcatcaatc tctaagtcaa gaccagttaa gtttttagta
     7021 taaatcgctt tgattttatg atccgtacct agactttttt catgacgtaa tgtgttatta
     7081 tcttttagaa actcaccatg aaatcgatct agaatagatc cctcttttcc accaaagaat
     7141 tctaaaaaat tcgccttttc tatcttcaca ttagcaagcg tatctactaa tgacgagaaa
     7201 gaaaactgcg aagggatagc tggtttcgct aaaacttttg cgttttgcca tgcctgagta
     7261 gcagtgatct tttctgttcc gctgtcatat ttattcaaca ccgattttct tatatcattg
     7321 aaaataggtt cagcttttac ttctatcgta ttgcctatta cagaagtttt tgcataataa
     7381 atccgtagac gctgttttgc tcgattttca tctacataac actgaataat acgtccgtct
     7441 acaatcaaat ctgcattagt tccgtttatt gaataagtac cctgaaatat ctcggctccg
     7501 tttagtttat tgctaacagt agctgttaac cagtctgaca aagcgcctaa accttgcgta
     7561 tcatataaat gttcagctaa attattcgcg tcatttttat cgtaaatagt tattaaatta
     7621 tcgatcatct atttcaccta ccttaacccg ttacgataaa tttgtatttt gctcaaacca
     7681 gtgcaattaa aatgattgat atccacttgc aatgtcggat attgcatggt cttcattttg
     7741 ttggaccgat ctaaaatatc tccgtccgat tgctcttcgt agcaaagcat caaatcacta
     7801 tcaatgacta catcagttcc tgctactaag ccttcgaaac taaacacata atcatttaag
     7861 atgaactggc atgaagtagc tgaaggagtg atgataatct ttggaaaact ttcttctaaa
     7921 ctattattca gcaagttaaa tgactgtggt ttatctacgg ttataggtac atcttcttga
     7981 actcttgcga atggtttcgc agtaatattt acatcgaact ctccccattc aacaatatcg
     8041 ttttctgcat ccccaatatc gatagtctgg ataacgtaat agacgttggg atcgtcagag
     8101 aattctaatt tctttgcata gtttaaccaa tgacgcatga tataaaacga ttgcttgaac
     8161 gcttgatggt cttccacatc ctctaaatag ttatagtgca atgtaaacga catatcttca
     8221 aacgagtaat cttgtactaa gccacctaac ctacctaaaa cagaagtttc aactctctgt
     8281 ctttttggag aaggtatggt tggtctttca gctaaagcca atttatgcaa ataatcagga
     8341 aatccatcga ttatagaatg tatacaatca gtcatttttt cacatccttt ttaatactaa
     8401 aaaaacagga gaaatactct cctgtttaac gccatgccga agcattatca ttttgaattt
     8461 ttgtaatgct atcaatgatt tgttgagttg tttgcttcat agtaacctca tctgcgttac
     8521 catcaattgt gaaattgaat tcgtagttat tcacaggttg aatcgtttgt gccctagatg
     8581 aaactgatgt gctactcaag atacgatcac caatttcttg caaaacagat cttttcaaag
     8641 gtaaaactgc ttcaggtcct gcttcaccga caccgttcat tccacctagt aaagttggtt
     8701 tagtaaagat acctccttta gcatgccatt ttacacgcaa atgggggatt tgacctttta
     8761 gcgggttaaa gctgccttcc atgataaatt ccggtaacgg aatatgtggt atagaaatat
     8821 tcaaattatc aaaaatgcca ctgattttgt ctctaatcca atcaattgga gcgctaacag
     8881 tctttttgat gccttcccat atgttagcaa ttgtactttt aacattattg aatatgtcgg
     8941 aaacaatacc tgttagattg gaccaaccgc ttgaaattgc attttttcca tcgtttactt
     9001 tagagctaat agtgcttgta attccattcc aaagattcaa agcagtattt ttgataccgt
     9061 tccaaattcc gctaatccac gaagatatac tattccaaac actttgaatg gcacttttag
     9121 ctgcgtttat agcattgctt atactactag tcacactatt ccagatattt gatgctgtag
     9181 agctgattga attccaaatt ccacctaacc aactagatac agttgaccaa atattttgaa
     9241 ttactgtagc agctgcttgt accaagctag tgattgtatt cttgatactg ttccaaatac
     9301 tagaagctgt tgcactaatt gaattccaaa tatttgaagc cgtagtacta atagctgtcc
     9361 atataccatt ccaccatgcc actactggat caaatatagt atggaatgta gttacaatcc
     9421 cattccaagc gatacttatc cattgtgtca tagtatccca agtattttta aggaaatcag
     9481 aaataggtgt ccaaacagct tgccaagctg cgcccaataa ctgtccagct acatcaaaaa
     9541 tacccacgat aatattaata ccagcttgaa tcaatgacgt tattaatgtc catggtattt
     9601 gaacaattcc tacaatgtct gcccaaataa tcgaccatac ttctttgact ccgttccaaa
     9661 tatttgaaac ccaatcaacg aatgtttgcc aagtttcttg gactccttgc cagatgttgg
     9721 aagctccttc aactaatccg ctccatagct ctccaaacca atcagaaact ccttgccaaa
     9781 tatcttgaac ccaatctaca aatccagacc aggtttcttt aaccccatcc caaactgatg
     9841 aggctccgtc ttttatactt tcccaagtat cacccaacca atcagtaaat tttttccata
     9901 aaccactaaa ccagtcagta attgcacccc agttcttaat tgccacaata acgcctgcta
     9961 taacagctat aattcctcca attataagca tcatcggacc aaataaatat gacacggcta
    10021 gtattgccgg taacagtatg ccaaatgctg ctgtaagacc acctatagca acaatgaaat
    10081 cctgtactgg ttgtggaaga ttattaaacg catcagccat ctttcctaga aaatcaatta
    10141 ctggttcgag tgcatctatg attgtgttgt ctataggagc taatgaatcc tttaattcag
    10201 ctattttccc gttcaactct tgcaacggag tagtagaatc ttcattcatt ttttgtgcag
    10261 atccactaac atcatcaaat gtatggttaa catcagttaa agattggaca actttcatcg
    10321 cattatcttc gccaagcgca gaccaaattg tcgaagcttt atttaattgg tcgtattgac
    10381 catccatatt gctaaaatct tgaatcatgg aattaataac gtctttttgt gttcctccgc
    10441 catttttcca ctcttcaaaa gcttttctag tactttcact aaacatatcc atgttttgct
    10501 caaatcgacc atctgttaac gatattccca tttccttaac taagtcattg actttatcaa
    10561 ggttataagc acccgcatct aaaccatttt gaagcattcc gaacgtttca tcagctgaat
    10621 atcccatttg actccataat tggctatatt ctgccatatt gtcgcctaat tcgtgcgttt
    10681 tatctaaacc gttttgagta cccgaaacca ttaaatccat tgcatcttga gcactcaagc
    10741 cgaaattgac cattaagcca tttacaccac gtaacgtttc atccatatca gcgcccatgg
    10801 tgttttctag gaccatagct tgttccgtga tattttgtaa atcttgatta tttaaatctc
    10861 ctaaattacg ctttaccaaa atcaatgcat ctgtggactg atctaacgat tctccaaaac
    10921 ctttataata aatgtctctg gctacattcg ttaattcttc agcctcttgt ttagacaaac
    10981 caaaattagc ttgtatctta ctctgggaac tacctacact gttagcagag tccactgctt
    11041 gtttccctaa ttctgtaagc ttatcgccaa tgtcgcttaa aacgtcagaa gcttccatta
    11101 aattattcat atctattttg cttccgatat cgtccaagtt agttgtatct acatttttag
    11161 cagcttgtcc taactcttca aattcacgtt cagcatcatt aagcttcgct tccatctgca
    11221 ttgcttctgt ggatgtagcg ccaaattcag actgtgtagc ttctaactgt cgtcttagga
    11281 tatctatcgt tttctccgca ttttcagatt gttgagaaac atattcttgg gctttcgcta
    11341 atttctcggc ttcagaagct gattgaccag cagttgcttg ccattttttg tattcggatt
    11401 caatcagaga agcactagct tgaacatttt tttgttcgct atccaactgt tgcattgtag
    11461 actcgtacgt ctgtatttcg ccttttgctt gagctagtgc attactcgtt ttatcaattt
    11521 cgtttgacaa acgttgttgc gctgtttgtt gattaatcag ttctctttca agtttctgaa
    11581 cttcggtgga attttctcca taatattttt tcgcattggc taaacgttgg ctagttactt
    11641 caactttttg actttgtaat tcatactgct tttctaaaga agataattta cttcctaact
    11701 tgtctgattc agaaccagtc tgttgtaatt gagcttgttc tagttttaat tctgctctat
    11761 ttttagttaa ttcagcactg atttctttta acgtagattt caatccgtca tcgttagcta
    11821 tgaaagttac ttctgcttct gttctctttt tagccatttt ttacctcctt tctttagttt
    11881 ttctgggatt ggtttattgc atagtttttc catccttcat aagcactctt gttgtaagcc
    11941 atttgcaaaa tgtcatctaa acagatatcg cttaaaacca aatctgaagg catagaaaaa
    12001 acgtcggtca acatcgaata gacatcgacc ca
//
LOCUS       DACDRN010000071        12000 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDRN010000071 DACDRN010000000
VERSION     DACDRN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.071"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1143..1541)
                     /locus_tag="IWJ80_002253"
     CDS             complement(1143..1541)
                     /locus_tag="IWJ80_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461949.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKYT
                     SMKPCILDWQWLIDAVLDVKCSKYNITLGC"
     gene            1757..2812
                     /locus_tag="IWJ80_002254"
     CDS             1757..2812
                     /locus_tag="IWJ80_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAP9461950.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2832..3176
                     /locus_tag="IWJ80_002255"
     CDS             2832..3176
                     /locus_tag="IWJ80_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461951.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDSKVAEVKNKPTDHNPIQQEHPSTVTSGHSAKKEKPML
                     KGFNAPSNKDLL"
     gene            complement(3357..3653)
                     /locus_tag="IWJ80_002256"
     CDS             complement(3357..3653)
                     /locus_tag="IWJ80_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461952.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETRSSLVDK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3657..4475)
                     /locus_tag="IWJ80_002257"
     CDS             complement(3657..4475)
                     /locus_tag="IWJ80_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9461953.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4695..4916
                     /locus_tag="IWJ80_002258"
     CDS             4695..4916
                     /locus_tag="IWJ80_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAP9461954.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            4918..5184
                     /locus_tag="IWJ80_002259"
     CDS             4918..5184
                     /locus_tag="IWJ80_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9461955.1"
                     /translation="MSYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5297..5836
                     /locus_tag="IWJ80_002260"
     CDS             5297..5836
                     /locus_tag="IWJ80_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461956.1"
                     /translation="MYNKSVNKLINLQKERFFMGWYGAYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKRQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            6327..6611
                     /locus_tag="IWJ80_002261"
     CDS             6327..6611
                     /locus_tag="IWJ80_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461957.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6618..6863
                     /locus_tag="IWJ80_002262"
     CDS             6618..6863
                     /locus_tag="IWJ80_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461958.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            6885..7139
                     /locus_tag="IWJ80_002263"
     CDS             6885..7139
                     /locus_tag="IWJ80_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461959.1"
                     /translation="MNYTDEEQITLEYVFTNEEGEEIEKVLIISTKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            7221..7406
                     /locus_tag="IWJ80_002264"
     CDS             7221..7406
                     /locus_tag="IWJ80_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP9461960.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            7473..7868
                     /locus_tag="IWJ80_002265"
     CDS             7473..7868
                     /locus_tag="IWJ80_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP9461961.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            7858..8235
                     /locus_tag="IWJ80_002266"
     CDS             7858..8235
                     /locus_tag="IWJ80_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461962.1"
                     /translation="MKSKKETEPMKSTHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            8236..8631
                     /locus_tag="IWJ80_002267"
     CDS             8236..8631
                     /locus_tag="IWJ80_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461963.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
     gene            complement(8937..9335)
                     /locus_tag="IWJ80_002268"
     CDS             complement(8937..9335)
                     /locus_tag="IWJ80_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP9461964.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(9527..10294)
                     /locus_tag="IWJ80_002269"
     CDS             complement(9527..10294)
                     /locus_tag="IWJ80_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9461965.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            complement(10329..11552)
                     /locus_tag="IWJ80_002270"
     CDS             complement(10329..11552)
                     /locus_tag="IWJ80_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9461966.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGRTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGTEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
BASE COUNT         4134 a         2004 c         1819 g         4043 t
ORIGIN      
        1 cgtgttaagt ttctttatat tactacgcgc cttgcgtata aaacttcttt attcatccca
       61 cgatccatgt tattttatat ttcctttttc atgttatttt tagccagaaa ccgatcaagt
      121 ttttacctat tcccaaaatc catttattcg ttattttttt ctgattaaga ataattattt
      181 gatgcttaaa ttagctaaaa aagcgatttg ttactgatat aatcatgata ttaaagctga
      241 attaggcaga aagataaaat gttcgcaaaa tcaaagtatc aattatcttg tttttttata
      301 aatttgtaag taaaaatcta aaaagttttc ttttaattgg tatatgtatt aaaatcacat
      361 tcaatcactc ataaaagtat gcgatttcat ttttttggat gagattaata tcaataaata
      421 acgatgtaaa aaccatttat tatgctcttt tataagggta ttaattgatc taatttgaaa
      481 aaataattca caatttaaat gttttaatat tacgaataaa ataatgttat cgtaaaatgg
      541 tataaaaaag ctaaatatta tatttttgtt attttattag cagaaaaatt taaatagttt
      601 tacaagaatc cgtagaaata tattggtttt gccttcaaat taatactgga tcatattaat
      661 ctgaatataa tatatgaact aataaatagc ttaacctagc tattttgttt tttctgatca
      721 ctatttatac agcattagta aataaaattt taaaaataaa aaaaataagg caaaaaaagt
      781 atcaacataa acatatacat atacacgttt atgttgatac acttaatttg tcctttaaaa
      841 agcacttttt tgatattatg ttattttata atcttattac aagctgattg ttaggcgttg
      901 ccctttcatt acatttatta acggttattt tatcgtttaa tttattaatt atttttgatc
      961 taaaacttag cttaacaaat aaataattaa ccaaccgcca gataaaaatg cgatcattgc
     1021 taatacataa gcaaagataa aaatatgttc gatcagctta aaccctgatg cgattaagca
     1081 gaccaagtaa aatagaaatt ctcttacaga taatggtaat tttctaacta gcatactcat
     1141 aattaacacc ccaaagtaat attgtatttt gaacatttta catccaatac agcatcaatc
     1201 aaccattgcc aatccaaaat acaaggcttc attgaagtat atttttcaga tgaaaagata
     1261 taatataatt tcttcggaat aatattttca gttgttggga tcaagctaaa tctaataccg
     1321 tctaactcat agctaccagc tttctctcca ttaaaaattt ccttagcact ttcttctaaa
     1381 gaatcaaaat atttttcgac agcaaccttt gaagctttta ctattgaagc atttcccttt
     1441 tcgataaaat ctgcttttgt tttaccgaca ataagtaatt cttgatcagt aaaatcttct
     1501 aaaataatgg tagtgtaatt cttatttctt ctcaatcgca tcaggatcac tccttaatat
     1561 aatttatgtt tttataatat cataataaga taaatttgca agcatttatt tacacctttt
     1621 tatttacact ttattataat taattatact tttatgattg catttttttc ttataaacag
     1681 ttgcaactaa tagtaaatag gtgtaaacta ttttcgtacc gaaataccat aaacacaaac
     1741 agaaagaggt cataccatga aagaaccatt attgttttcc gttgacttag ggaataaatc
     1801 agtaaaagaa atgagtacac acgtttctcc actctcactt ccctctcgta ttttgaacaa
     1861 aaatatcatt aattcaggac gtttttctaa tataggttcc tcattaaaac gatcactaaa
     1921 cgtttctgaa tatagcctag taaacactac tgaaagctat tactttggga aggatataca
     1981 cgagctagga aaagatgact acgtgataga atcactaggt tttgggatca gcagatacaa
     2041 gcaggaaacc taccaaaata tcctatcttt ctctttagct aacctagcac gtttagaaga
     2101 tcaatctgat gacgttttaa ccgttaatct agttttaggt ctgccttcat ccgactatac
     2161 cgaaacaatc gtcaaatacg tgatgcaaca agccacacga caacattcag ttgaagttga
     2221 cggtaaggct tataatataa acgtagatac cgtttgggta ctgccacagc cgattggaac
     2281 actatataac ctcctacttg ctgatgatgg tagtgtacaa gatgaaacat tgatcagtca
     2341 gaagatcggt tttgttgata ttggcggtgg cacgaaactt tttgatgttg tccaaaattt
     2401 tgaaattgac gaaaacaatc gtgaacaaaa ggataccggc atttttactt tatataatcg
     2461 tatcgtaaat atgcttgacc ttccgatcag tcctaatgct ttccaacttg aaaagattat
     2521 ccgagaaggc aaagaaaaag gcgtatatct atttaagcca agcggaaatg aaagttttga
     2581 tatttcagaa gtagttgaaa gagaaataaa aacatacacc aatatggttg ttgcagaagt
     2641 tttagctaca ttcaaaaact tcacaacctt agataaagtt gtctttactg gtggtggagc
     2701 ttctattatc gacaaacaac ttgtaaaaga taaaattccg cataccttgt ttgttgaaga
     2761 cagtgaattt tctaatgtaa aaggctttta caagtttggt ttaactcaat aaaacagcga
     2821 aggagatcaa catgaataag ggaaaatcag agcgttatgt agttataatc aatagagaaa
     2881 aggttagcga gttaccttta gcagagtttc ttgaatccga atatgaaaca ctaggcgtta
     2941 aatactctca gacgatcaaa aaagctttga acctatatcg gcgtgtaaag aacaatgaag
     3001 ttacaattat tgaagcagga aacgatagca aagtagcaga agtaaagaat aaaccaactg
     3061 atcataaccc tattcaacaa gagcatcctt caacggttac atccggtcat tcagcaaaga
     3121 aagaaaagcc aatgctaaaa gggttcaatg caccaagtaa caaagactta ctttaaaaat
     3181 aaaaaggcga ggttacaata cctagccttt ttattatgta caaatataaa cataaacata
     3241 tacgtataca tgtatataaa catgtatacg tatataacca taaacacgta tatgaaattt
     3301 agttaagcta tacctaaccg cttattccaa taaaaaaatg atcagcttta aactgatcac
     3361 tcaccaaaaa cttcatcata caagttatct atcatttcgc caagtaaagc tgaatctgat
     3421 cgaattccta acttttcgct gattttagat agtttctttc tttgcgatgg ttgaagcgtg
     3481 aattgataat ttttcttctt ttcagaacta tcacttttta gtttatctac caaagaactt
     3541 cttgtttctt taggtgtttg tttaggttct tcaactgctt caaccacttc aggaacttct
     3601 tctacttcag cttcagcgaa ttttttaaat ctattttctt tcacttcaat cactccttat
     3661 tcttgtgccg tatcaattat tttgttgtat agcgtaaacg tgctttcaaa gaaatcctta
     3721 ttcttacgat agatattctt atccttagcg aactcagcaa tattcttacc ttctaagcta
     3781 gttgcattaa gcaattcctt ctcttgtaca tatccaagat aattagataa cgttggtacc
     3841 aattctagga actcacgact tgaattagta ttatgtttga tcctgctacc gatcgtagcg
     3901 tatttagttt ccatcagtgt ttcaccagtc ttaatatcaa tcaactgttt tgctagtgca
     3961 tcaatatctt taccaagctt cagcaaagct tcaaaaccat ctttagatgg atcagaaatg
     4021 cctacgacaa gatcagatac agcccaagca ttttgagcta gtagacctgc atcattatga
     4081 gtatcaatca gaattagatc atacttctct ttaagctgtt catagttaac aacgatccat
     4141 ctaaacagta gagttctgtt gttaggcata tcacgcattt gcatctcaac ttgttttaaa
     4201 tcttcacctg ctactaaaat atccatattc tcatttatag ctaaaggttc agctgatcca
     4261 tgaagatata aatgattgat tgtattctct cttttcgctt tactgaaagc atcaggaaag
     4321 cgttttgata ggttttgcga cttatcttca tcaatataca gcaccttcat acctaaattt
     4381 tcgacagcat atccacctag attgtagatt gttgttgttt tacctgttcc acctttttca
     4441 atgtttccag taatgattga caacttcttt tccatgatta aacctccctt attaaccttt
     4501 tgtaactcca ttatacacta taaacacgtt tatgtatatg tttatataca taaacgtgtt
     4561 tatatttata tttgctttta tattctacaa cataaacatg tatatgtata tgtttatgtg
     4621 tatgttcata tatataacta ttgcatttgt catacaatcg tagtacaatg taattgaaaa
     4681 ggagttgata aactatgcca gtaataagta ttcgtgtatc taaagaagaa aaagattttc
     4741 ttgaaaaagt tgctgaattc aacggagata acatttctga atttatgcgt agtaattccc
     4801 tgcaaacagc agaaacgtta gtagattttg ccacctacaa ggaactaatg aaagagcatg
     4861 aggaaaacga tcaaagtatt tcccacgaag atatgctaaa agagttaggc ttataagatg
     4921 agttaccaag taagatatga acgtgaagct caaaaagcgc taaaaaaaat ggataagttt
     4981 caggcaaagg ttatcctaaa ctggatcgag aaaaatcttg taggcactga tgatccaaga
     5041 aagcatggta aaggtctaac agctaataaa tcaggctact ggcgatatag ggtaggtgca
     5101 taccgtataa tagctgatat tagcgagcat gaagtgacaa ttcttatatt gtctattggt
     5161 cacagaagtg aagtttataa gtaacagaca agctgataag gcttgtctgt ttttttgtta
     5221 acccaacaaa atcactgttt acgatcccac tttacctaaa aaagaacaat atcctcttgt
     5281 tattatacac attggtgtgt ataataaaag tgtaaacaag ctaattaact tacaaaagga
     5341 gcgatttttt atgggatggt atggtgctta ttcatcagca aatgaagtga tcgaagaaaa
     5401 gaaagggtac gccaaaaatg gtttggaaat cgtagatacc aaagtaacaa agacctatgg
     5461 aattatcctt ttcaaagatg cagaaggaac ctacgatatt gacagcttca tctttcgaga
     5521 tgaaatgtac aaaccaatct acttcttaaa tgacagtcct gcttacttta agaaaattcc
     5581 gaaaaaatgg ttgaaattct ttgtcggtga tcaagaaaaa atggttaagc gacaactaga
     5641 tagaattaaa gaagaaagca ataaaccaaa acttgatgac ttattagttg caggtaaaac
     5701 ttactcaatc tgggatggat cgtatgtcgc tgtttatcag tataaactca aacgtagctt
     5761 tatttttcga ctacaagatg gatcactcac tcggttcact cgactatcag caggagatgt
     5821 taaggaagta gcctaaaact taaatacata aacataaacg tgtatacgtt tatgtttatg
     5881 tatatgtata tgaatataaa cgtattaggt acgaacgctt ttaaatgctt ttctgggctt
     5941 tgattttagg gtagagctaa aacatcacca tatgatccaa aacagctcag aaacgcattt
     6001 aaacgtatct gataccaata ttatagaagg agtgtatcgt atgattaacc tatttctttt
     6061 ggtgacagct agtacattat tctttactgg aatatcactt ataatttcct atttgatcta
     6121 taagaaagcc gaaacttaca caagcaaagc aaccggacta gccgtttcat cctttttaac
     6181 tttgctgact ttttttgtta ctctatcgtt ttcttttgtt tagcacagct gatccatcag
     6241 caaaaaacct ctctttacat tatacacact agtgtgtata atgtaattgt aaacaagcta
     6301 attaactttt aggaaggagt gacaatatgc taacagctac attacagcaa cctactcacg
     6361 ttgctattgc agaaagctat acaattaatg cagggatcgt taaacagctg atcaaggatc
     6421 gagaattcaa aaagtttaac agtcgtcaag cgtataacag acagttaaag aaagctattc
     6481 taaagcacaa aaattccttc atcgggaaat tggaaagtgt ggatgttaaa actgtttcaa
     6541 cggcattttc agttgttgaa gtgactattg acggtcgctt gatttctcta tttgcttttg
     6601 gaattaattg atagggagtg tggactttgg aaggtaagac ttttgcagaa atttggactg
     6661 aatttattga ggtaccaact cgcattgatc cgataacttc tgaaaaggta ttggatgcag
     6721 acttctacaa tctcaaagca ggtactagcc ttgaatgcgt ttggatgtat ttagaagagc
     6781 atttcaaagg cgtaacgctc ggtcacgtct tataccacaa tggtgccgag tgggatgcaa
     6841 ttggaaatat ccatttttct taatacaaga aaggagaaca caatatgaat tataccgatg
     6901 aagaacaaat cactttggaa tacgttttca caaatgaaga aggggaggaa attgaaaaag
     6961 tcttgatcat ttcaacaaaa gacttggaga aggctttggc ttatgaacca tacgaatcaa
     7021 ccgtaactgt cattgaattc ttagatacct atacttctaa tgatacttgg gaaattatga
     7081 atatttgcaa cgctaataac attcaatttg ctgaaaaaac caataaatca ttttattagt
     7141 tttgcaataa aacatcctct ttttctattg attacataca caacaatgtg tataatagat
     7201 tttgtaaagg gagggtagct atgcctaaga cagcaagaga aattgtaaaa attctcaaat
     7261 ctaacggctt caaaaagact ggacaaactg gcagtcatgc aaaattttat aattccaaga
     7321 ctaaaagaac ggtcgttgtt ccgatccaca atggtgatgt accactagga acagaagcta
     7381 atatctggaa gcaagcaggg ctttaacagc cttgccttcc tatttggtat taggcatact
     7441 atcctctctt acactagaaa aggagcgagg aaatgaagac aaaaaatatt ttttatccgg
     7501 caatcttcga gaaagaagga gaagcttata acatcgtttt ccctgactta ccggaaatat
     7561 ctacctttgg aaatagtctt gaagaagcat atttaaacgc taaggatgct ttaggattag
     7621 cgctatatag tgtaccagat gcagaatttc ctaaaccatc agcagtggaa gaattaactt
     7681 tgaaagccaa ccaagtggta gtaatcatcc aactaaacat taaacttttc cgaagaagct
     7741 taaatacaaa aacaattaga aaaaatgtta gtgttccgga atggttagtt cttttaggga
     7801 aggagaaaaa cataaacttc tctcaactac ttcaaaaagc actagaagaa gagttgcttg
     7861 aaaagtaaaa aggagaccga acctatgaaa tctacacact taaactattt ctctcgaacg
     7921 ttaatcaata atctattaaa agatacaact ggcattagcg ttagtaccaa acactttgcc
     7981 aatgatctaa ttatttttga ggataataac ttgcgaattg agatgtttat tgaacaggca
     8041 acccctaact caatacctga attctgctat acgcacactg aaaaaatgaa acatgcaaaa
     8101 tcagttaaag atgttgaagc attaagtgaa tcactaacga acgctttaaa cctcgttaag
     8161 aaggcaaaat tgttatttga atttcaaacc gactaccaca atgagttgct tgctcaatct
     8221 gaaattttag attaattgaa acaggagaag agaaagatga aacaaacaca aacagaaacc
     8281 gttaacgata cgcttgctga tcaattagca ggttattata aattatttga gatgggtaaa
     8341 gcacagcgct tgatccacta tctttggaaa tacaaaattt tgtttctttt ttgggaatca
     8401 acactaacat tctcagttct tttttataag tcagattttt ataatcaaca cttagtttta
     8461 gggagttttt tgcttatcta caccattttg atgcttaatt tatataccta catcacactt
     8521 gaagaacact tagtaattag cgatgcaggg atcgagagaa acatcaaaag ggtatctttc
     8581 aagatcacgt taaacaagcc taccgataaa aagctctcag aagcgttctg agagcttttg
     8641 acattttctt gtataattta tcctaacaca ttataaagca cctaattcga gcttatatca
     8701 agcaaatttg ctatttatta cttaatacat atctaaaaat tacttgatgg aaaactagat
     8761 acatggtaag catgaaaaga aaatcaattt gagcagatgc agtacaatac atagcaattt
     8821 aaaagctctc agaaggcttc tgagagcttt taatatattc tttgataatt tgtctttctt
     8881 aaatccatac gtcctaaaac aagctgtttt tgcttaatat gatcatctga gagacatcaa
     8941 atcaactttt ctctcaacaa atcattcaaa gctttcgaca gattaatatt ctcttcagcc
     9001 gctaaaacat ttaaatagtg ttccacagtc actgattttc gatcataatt ttgcttgttt
     9061 cgagctttag caactttcgt attagctttt acgacaaata aattaacatt ctcagatcgc
     9121 ttgattcttg catttaactc ttgaacaggc gtaggcgatg gaatttcttt ttcttgcaac
     9181 tctaaaagca ccaagtatcc ttccaatact tcttgcgcca tctcaaaagc atcttctaaa
     9241 gtttcaccat gtgtatataa gtctggaaaa tctgggaatt taactgcata ttcatttcct
     9301 ttccattcga atactgcaca aaaaaagtat ttcatcgcat tgcacctctc taattaaaaa
     9361 ataatttttg cttttttgtt tcaaaaaaaa gaatcgccta ccaaaaaaat tgataggcga
     9421 tcacaagcta attaacctct aaaggagatt aataccttgc tcccaaggca ctatatatct
     9481 tagcatacgt ctgaagcatc ttcaaacgag taagatatat tttgcattaa cctaattttt
     9541 tttctctata agttttttca tcaaactcac tagaaatatt aaactggaca gcaaaattgt
     9601 aatttctgcc ttttctaact ttagcaaaat caatattcag cattgatttt tcattaattt
     9661 cctcaactga ttcccttaaa acacgtctat caaaattcga tattgtttta tactcatttt
     9721 ctgttttcgt aatctcacga attttaacta tttcaatcag gggattctta tatatttcag
     9781 ctttctcaaa atctccttta tttttggcga taagatattt tttataatac atcgccaaat
     9841 atttgtataa cacgatgcta tatttttttt gaaaagatgc taagtcttca aaaacaaaga
     9901 acggatactt ctttttgatt gagacaatta agggaattac atcttctgaa aaactgaaag
     9961 atacactatg ctcttctaaa tcccaatgaa tccgatcaat agcagataca gtagtatacg
    10021 tcttatattt tttgtctaga ttcgaaattt cataaactgc ttgtctttgc aaagtgttaa
    10081 ctatggtttt tagttccgtt tcaagatttt gatcaacatc catgtaatta ataatcgctt
    10141 taacaggtaa atctactacg cctgttttct ttagctttgt gggtgagata taactgaaca
    10201 gcaattgata aagttcatac gactttaaat ccattttcat gtcaattgtt gctaagaact
    10261 cctcattgta ctttgtcagt tcatcttttt tcaaaagttt ttccttcctt cacgcatttt
    10321 ttcagttgtt aatcataaag gttcacataa ggcaaataac tcgtaattga tttttcgaga
    10381 tatttaacag tattttttat tgtgtaatcc tccttatgag tagcaacata agataggtga
    10441 ttgacaactg attcaaatcc tctatctttg gataaacgat cataaagcgg ataaacttta
    10501 ttctgaagtc ggctcataag tttaagatcg cataagtcgg cagaactgat caaaaagttt
    10561 ttgattagta atttagtgta tttgctattc atagctttca agtacgattg attcattttc
    10621 tctgttttat cttctgtgcc ttttaaataa gcagggtctc tttgttcttg cttataaggt
    10681 accggaactg acatggtacg tgtaagcaag aactttatat ttgtataagt acgacccttc
    10741 ttgataagct catagtcaat tttaaaatca gtgtatttat tcagttgggc aatcggttct
    10801 ttaattacac gtctttcaaa gtctcgaaaa gctttatatt ttttctcaat actgaatttc
    10861 tttcgtaaat ctgcaatgga gaaaacagga ttttttaatc tatcaagtga ttttgtactc
    10921 catgttaagt tatctctatt tgcttcatac agattaaaat ctttagtgaa taattgaaaa
    10981 aaactgattg catacttgct tgtcaagtta actacttgat caaatgtata ttgcgtaaaa
    11041 tgatcctcta agttcgttaa taaaggcata attgaactac taaatttaat agatactgca
    11101 tcagaaaaat cattgaattc agtaatttct agcggtgaga tgactttgta ttcagtctta
    11161 ccgttaggtt tagttctttg aattttaatg acagattgct gatgcaattt ttcaataact
    11221 tgttttaatc ttgaatgttt tgaagaagat gaactagaag acatcaacgc aaataaagcc
    11281 tttttagata ctttgacggt attattaaaa ggcggatttt tagggtcaag atgtcctata
    11341 gcgtaaagat aaattttttg tggcatagca tccatttcaa caatgctatc tacaagttga
    11401 ttcgataaag taacagttaa atcaaacttt tttagattgc tttcggtagt ttcaaatttc
    11461 gggtttacat tcttttctgt tattttcata cgattgcctc ttctttctaa gcttggtacg
    11521 agcttaaaaa cttggaactg gtacgattcc attgaataag tatagacctg ttttcgatac
    11581 gttacaataa gaaatgtatc gtaaatttat aacataactc aatgtcagta aaacacattt
    11641 tattttttcc aatcaaattt cattgaaaat acagcagaaa tcgctatttt agaacatttt
    11701 caaaataaaa atccttttca aaaaaatacg aaattataaa aattaccaaa attaaaaaac
    11761 aaatattttt ctagttttta gaaataaatt cacatacttt ttcttttttt ggatatattc
    11821 ggaataccac atcgcttttt gtccacattc acatcgcttt atgtccaccc taaaatctat
    11881 ttaagattga tataaagcct ttttggaggt tgctaaagtt ttaaagatta aaatatatat
    11941 tataaatata cccgtggtgc tagttaattt taatatccta aagaatatct caaagttcct
//
LOCUS       DACDRN010000072        11783 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDRN010000072 DACDRN010000000
VERSION     DACDRN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.072"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(52..1392)
                     /locus_tag="IWJ80_002271"
     CDS             complement(52..1392)
                     /locus_tag="IWJ80_002271"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP9461967.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(1629..3152)
                     /locus_tag="IWJ80_002272"
     CDS             complement(1629..3152)
                     /locus_tag="IWJ80_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP9461968.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(3531..4142)
                     /locus_tag="IWJ80_002273"
     CDS             complement(3531..4142)
                     /locus_tag="IWJ80_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9461969.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(4265..4390)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(4472..5188)
                     /locus_tag="IWJ80_002274"
     CDS             complement(4472..5188)
                     /locus_tag="IWJ80_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9461970.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(5194..5763)
                     /gene="scpB"
                     /locus_tag="IWJ80_002275"
     CDS             complement(5194..5763)
                     /gene="scpB"
                     /locus_tag="IWJ80_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP9461971.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(5747..6544)
                     /locus_tag="IWJ80_002276"
     CDS             complement(5747..6544)
                     /locus_tag="IWJ80_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP9461972.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(6514..6885)
                     /locus_tag="IWJ80_002277"
     CDS             complement(6514..6885)
                     /locus_tag="IWJ80_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9461973.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(6904..7791)
                     /gene="xerD"
                     /locus_tag="IWJ80_002278"
     CDS             complement(6904..7791)
                     /gene="xerD"
                     /locus_tag="IWJ80_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP9461974.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(7887..8360)
                     /locus_tag="IWJ80_002279"
     CDS             complement(7887..8360)
                     /locus_tag="IWJ80_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9461975.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(8465..9328)
                     /locus_tag="IWJ80_002280"
     CDS             complement(8465..9328)
                     /locus_tag="IWJ80_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9461976.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(9524..11623)
                     /locus_tag="IWJ80_002281"
     CDS             complement(9524..11623)
                     /locus_tag="IWJ80_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9461977.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT         3162 a         2500 c         1982 g         4139 t
ORIGIN      
        1 aaaaaagacg gaatatccat ttgaatatcc gccctttttg tatgtttatt tttatttagc
       61 acataaacga tacacagctt ctgcataaat ttccattgct ttatacatac tacttaattc
      121 ccatcgttca ttcgcctggt gcataaagtc aggagtatca gggaacatcg caccgaatgc
      181 cacacactgg ttcatcgtac gagcaaaagt agcgccgcct gatacaaacg gttcagtcat
      241 gtctccagta atatcgcgat atgtccccaa tagatttttc actaactcac tgtctagtgg
      301 cacgtataat gaatcaagga agtcaaattc ttcataagtc aacccatatt tttctgctgt
      361 tttagttaat ttagctacta aatcatcttt cttgaatgta acagggattc tcatatctac
      421 accgattttt gtttgttctg gtgtgatttc caaacttgca aagttcatcg ttagctcgcc
      481 ggattgttca tcttctgttt tgcccacgac agcttcacca gttgcatttt cttgaactaa
      541 ttttcctaag aaattgattg ggccaaaatc aaatacttct gaaagagcaa ttgccagacg
      601 agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg atacttttac caagaacgac
      661 gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt ttcaatgctt cttttacttc
      721 agaaagcttt tctccagagt aaggagctgc atcaggaaca acattcaacg ctcctccagc
      781 ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa gcttgcaata agcctttttc
      841 tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct tgcgtaattc cttcttcttt
      901 ttcgttatat ttttctaaac aacgccataa attttcttca tctgtaccaa agataaaacg
      961 aatacgtgtc ttgaattcca cgccagcatc aatcaaagct ttgacggcat acattgccgc
     1021 aatagaagga cctttgtcat cttgggatcc gcgaccaact aaccagccgt cttttatagt
     1081 tggatcgaat ggttttgttt cccaattatt ttcgtcccct gctggaacaa cgtccatatg
     1141 acacaagata ccgaataatt catctccaga gccaatctct gaataaccgt aatagccttc
     1201 tggatcttta aatgttctaa atcctagctt ttcactgatt tccaatactt tatccaaagc
     1261 ttcaatcact tttttaccga agggatgtcc ttgtcctgaa tctgcttcat ctagtacgga
     1321 aggaattcgg atcaattcgc tcaatgattc taatgcttgt tcatgatgtt gttctgtcac
     1381 aaattttttc atgatattat cctcctaata tgttaaaaag ggtcatgtca aattcctccg
     1441 acacaacctc ttcttctcaa ataatgacgt tttaagcctg ctccatcagc aagcagaatg
     1501 ttcttaacaa atgatgaaag aaaaaacact cactgaatgg agctattgac ttatccacgt
     1561 gcttatcgat agccgtttat cggtttcttc attcttatga tagaccttgt acctgtattt
     1621 gtctaggttt agaacattgc tgctacacct aggatcacac agatggctac aaataatatc
     1681 gcaattaatt taccggtaaa tttccaccac actgaaacat cgaaacgagc aattgctaca
     1741 gcacccatta cgatagcaga tgttggtgta attaagttta ctaaaccaga tgcagattga
     1801 taagcagtga ttactaaatc cttaccgacg ccggcaaatt cacccatagg tcccatgatt
     1861 cccattgttg cagcagctaa gccggaagtt gaaggaatca agaatgacat agggatatag
     1921 aaaataaatg taataatgat gaatacactt gaagataatg aacttaatcc ttgttcgccc
     1981 caatgtaaga tggtgtctgt gattaatcca ttgttcataa ccacttggat accacgagca
     2041 acagctacta cgattgctac accgatcaaa tcagaagcac cagctaaaaa ttcagagaca
     2101 aattcagact ctttcatgcc ataagtgatt gctactacga tggacattac taagaataac
     2161 atagtaattt ctgggaagta ccagtcacct aatggaagca tgcttttacc tagaaccgta
     2221 cctagtaccg gaaggtttgt taaccagttt gtcaatgatt caaaaatcgt gaaattcgca
     2281 ttcaaggttg tccaaggaat caaactgatg atcatgatac caaaagtaat aaagaacaat
     2341 acattaacat gtttttgttt tttcgtgatt acttcttgac gaccgattgc ttcaatatcg
     2401 aaatgcttca tatcttcttt acgttggttg tatactaatg attttgttgg atctttttcg
     2461 atctttgatg cataacgata cacatataca atagaaatag ctaacaaaat aacgaacatc
     2521 aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa cagcttgtga agctacgcct
     2581 gttgcaaaag gatttaccgt tgaagctaga cagcctactt gagaccctac taatacgatt
     2641 gctacagcta ctactgtatc aaagcctaca gccatcatga ctgggatcag taaagcatag
     2701 aatggaatcg tttcttctgc cattccgtaa gtagagccgc ctaatgcaaa taacagcatc
     2761 aatactggaa taagcatttt ttcttttcct ttgaatttct tcacaactga agcaatcccg
     2821 gcatctagtg cccctgtttt gttgataata cctaaaaatc caccaacaac caaaatgaag
     2881 aaagagattg ggattgcagc aggtgtttct ttagttagct cccccgctgg tacacctagc
     2941 atcccattga ttggcgccat gaaaatatcc cacagccctt gaggattttg ttgtactcgt
     3001 tcatatgatc cagcgacgat gttgcctgca tcgtctacac tatagtgccc agctggtata
     3061 aaccaagtaa gtacagcgat tactgcaata ataataaata agaccgtata cgctgaaggc
     3121 atttgaaatt ttttcttttt tgtttgttcc atcttcttct tcctttcttt tccgttagag
     3181 acaacaattt tggctctaat gttattttat caaaaagagt atcagaacaa aaataataac
     3241 acgcattttg taaaactttt gttcaaaaag ttcgtttttc cctattccct cctctttttt
     3301 caggttttta aagctttctt ctttttcatt gtagcgtttc ctttttttta taaacaaaca
     3361 ttttcgtctt ttaaaaggag aagaatttca catttgcaaa gaattgattg atttttatgc
     3421 ataaatcaaa aaaatactca aaacaaacaa ataaaacgtt tacattattg catgtcttgt
     3481 atttttttcc aaaaaaaaaa gaccaagaaa tttcttgatc tttttgatat ttatttgact
     3541 gctgtatgac gttgttcgaa ctgatgttgt tttcgactta accatggaag aagttgcgca
     3601 tgcagcacta aaaagacagc actcacgatg aatcctttga tcaagttgaa aggaacgatc
     3661 ccaatcagta catagtttgc taaagacatg cccagcatct gtgttgcgtt cagccctaaa
     3721 aacattaagt aaagaggcgt aataacgaaa taatttgcga tactcataaa aatagttaga
     3781 gcgatggttc ctgtaatgac acctaaaaat ttgtttcttc ctttatgctg tttctttaca
     3841 ctaaagaaat aataaatcgg gaatgtaaag atcgttgttg ataagaaact agcagtgtct
     3901 cctaccaaat tatccggaga aaaacctgta gtcagcagat gtaatactga acgcaagaac
     3961 gctgttgcaa ctccggccgc tggaccgaat aagaacatgc tgatcaatac cgagatatca
     4021 ctaaaatcga tcttcaaaaa actaaaagcc ggcatgatcg gaaaagcaaa atactgtaaa
     4081 actaatgcga tcgcagcaaa catcgctact gcaaccattt tctgtacacg ggtgttcttc
     4141 atttgaaatc ctcctgttag gactcctctt cagcgaactt tttgacgttt ctctccaaat
     4201 gaaaaaactc tattttccag ggaaaataga gttagagtta tttgatcaag cgtcgtcaaa
     4261 taagctctat cttctttatc cagactatac tgtcggtatc ggaatttcac cgattcagct
     4321 atttttttgc aaaataggtc gtggactata accaccggtc gggaatttca ccctgcccct
     4381 gaagacgaac cttatataat ttttgtttac atattcatta tacatatcta ttcaaaaaac
     4441 gcaacatcta acacttataa aaagtaagtg ttcattttga gtggttcagc aaactgctga
     4501 cttctttttt gttcagttca cggtaatccc caggacgtag gccttgtaaa gtcagatctc
     4561 cgtatttttc acgttttagt ttttgcacag gaagaccaac tgcttgaagc atatttttca
     4621 cttgatgatt acgtccttcg tggatcgtca attccacgat actgtggttt gtcttaggat
     4681 ccactgataa aatttggaat ttagctggag atgtttttcg tccttcgatc tttattcctt
     4741 ttgtcaaagg tgcaagcatc gattttgtag cgatcccttt tacctttgca acgtaaactt
     4801 tatccacttc atgttttgga tgtgtcaggc gctgagcaaa atctccatca tttgtcagta
     4861 gcaagatccc cgaagtatcg taatccagtc gacctacagg atagatacgt tcttttacca
     4921 tcggcaaaag atccgtaaca acttttcttc ctttgtcatc tgcaacagca gaaataactc
     4981 ctttaggttt gtacaataaa tagtaaccgt attcttcttg ataaattggg acttcatcca
     5041 cttccacagt gtcatgtttc ccaactttta tccctaattc ccttacaact tgtccgttta
     5101 cttttacacg tcctgcaagg atgaattctt ctgcttttcg acgagaagtg atgccagcgt
     5161 gggcaattac tttctgcaat ctctccatta caattattct cctttattca ttccatcaaa
     5221 gaatagatct ggtgacacgt cttcttcaag tgaaatttcc atttcttgtg tatcaggcag
     5281 ttcttctaat gatttcaaac caaaataatc taagaagtag ccagttgttc catataatat
     5341 cggccgacct ggtccttcta ctctaccttt ttcttcaatc agtttatggg cagctagacg
     5401 ctgtaccgct cctgaaacct gaactcctcg aatctgctcg atttcagctc ttgtgattgg
     5461 ctgtttataa gcgatgattg ctaaggtttc gacggcggct tgagataatg tgtttgatgt
     5521 cccttgcgca tatcgcttaa gtaagggagc cagctctttt ttagtcgtta atacataatg
     5581 gctttccgtt tcaaaaatat gcaaagctga gtgcgagttt tgagtgtaag attcttttaa
     5641 ttctttcact aaacttgtga cttctgcttc gctgagattt aacagtccgc ttaattcttc
     5701 attactgatc ccttcctcgc caacaataaa aagaagggct tcaagttcac ttaatttcgt
     5761 catgttcggt atctcctact gctggataaa gcataattga ttcatagttt tctttttgtt
     5821 gcgcaatgat ctgccctttt ttcatcaatt ctagcaaagc cataaaagta gtgacaactt
     5881 cacttttaga aggtgactca aaaaaagaat cgaaagtaca gccatgcgca tttcttttta
     5941 tacgctcttc gataaatacc attcgttctt cgatcgttgt gtcatcgggc gtgatcgttt
     6001 tttccacttt ttgagaaaat ttcttacgtt ctaagatttt ctgcatggca cggatcaaat
     6061 cgattttttt gacttgtcct cgcttcaacg gcttttcagc atccgctaac ccatcgatgt
     6121 tcataggttc ttttgtgtaa taaagacttc gttcctctgc tttttctgtc agtctttcag
     6181 ctgcatattt atatttacgg tattctaaaa gctgagtgac caattcttct cgaggatctt
     6241 cttcccataa ttcttcatcg tctgtgacga attcttggac aggaagcaat gtcttgcttt
     6301 tgattgccat caatgtagca gccatcacta agtattctcc tgcaacagct agctcgagtg
     6361 ttttcattgt atggatataa ttcatatatt gctccgtgat cgtagcgatc ggtatatcat
     6421 atatatcgat ttccatttgc tgaatcagat gaagcagcag atcgagagga ccctcgaata
     6481 catctaattt aatattgatc tccgtcaact cattcactct ttcttctttt tctttgctag
     6541 ccattttcca atgtaaggct ctgtgatttc agttgcacgg atggagacat ccgggtactt
     6601 ttcctgtaat tcatctaaca tttgaaagcc catattctca ccgcgataag acggattcaa
     6661 agaaatgtca tggatgatca gctgatcgtt cgttgcccag taaataccaa taatcccttg
     6721 aatgttggtt gaaccttctg gcgtaaagaa aaaaagttca taatcgtctt cagattcgta
     6781 cgtatcaatc tccttcaaca aagaagtttt acctgtcagt ttcttatgga aactcaatag
     6841 tcccatagca attttacggt tttctttccg gtaatgtgtt aacatcttct caccttccta
     6901 tgactaagcc cttggaaaat gctgcttata aacatccgtc attcttttct tcgtgatatg
     6961 tgtataaatc tgagtcgtag agatatccgc gtgacctaac aattcctgta ctgtccgcaa
     7021 atcagctcca ttttctagca gatgtgtggc aaagctatgc cgcagcgtat gtggtgttac
     7081 ttctttgtaa atcccagcct ctcgtactaa ctgcttcaga tttttccaga ttccttgccg
     7141 agaaagacct ttcccatgat tattgacaaa tacatgcatt tcagatgcgt cctttacaag
     7201 taatggacgt gcttcgtcta agtagcgttc cagccattgg atcgcataat cacctaaagg
     7261 aatgatccgc tctttatctc cttttccaac tgtctgtacc agtccaagag aaagatgaag
     7321 atctcccaat ttcaatccaa tcaattcact gacgcgcatc ccagtagcat acatgacttc
     7381 caagattgca cgatctctta tccctaacgt ttttgtcgta tcaggtgctt caatcagctt
     7441 ttctacttct gtcagagtca atgtacttgg taatttctgt gctttcttcg gcgtatcgat
     7501 atgttgcatc ggatcatgat cagtgatacg ctcttgccgt aaaaactgat ggaatcttct
     7561 gagacttgag atcatccgag taacagttgc tgtcgctttt ttttcttcat gcaacttctc
     7621 cagaaaacgg atgactaaaa agcgatcgac atcctgccat gaagcaattt tctgctcctg
     7681 tagaaaatgc aagtactgtt ccaaatcacg ttcatagctt tgtcgtgtat tggcggacaa
     7741 tccccgttca atattcaagt agtggagata atcgttgatt tgctctttca taataatccc
     7801 cctcatgcct ctctatcata tcagataccg cttcattctg atagtctttt tccataatct
     7861 gagcaaagaa aaagccgtct agacactcag acggactggt ttcttttttc tttttcatag
     7921 cattcctgac agattccatg gaacgttagc cgatgatctt ttactaaaaa atgataacgt
     7981 tcttctacta cttgctccac ttcaagtaaa agatcctctt ctacttcctc gatcgtgccg
     8041 cagttcaagc atagtaaatg atgatggaaa tgtttggcgc cttcttttct caaatcataa
     8101 cgggctaatc catcattaaa acttacttta tcgacaattt tcaattctgt caagatttca
     8161 agggttcgat aaacagtagc aagtccaatt tccggactct tttttttcac taaaaagaag
     8221 atttcttctg cagataaatg ctctttctca ttttccaaaa gcacaagcag agtagcttct
     8281 ctttgcggtg tcagcttaaa ccccgcttca tgtagctgct gttttgtttt tttcatcgcc
     8341 gaaatagcat tcatgaccat ttactcaacc ccaacaattt ataataatta caaataaaaa
     8401 aagatataac actatgttaa ttaaaattat tataagctag ttaagaaaaa catgcaaggg
     8461 attctcaatt agattttttg actagttttg tcacaccgtt ttcttgtgta atcaatcctt
     8521 gtttcaaaag attacctaat gcgcgcttaa atgctccttt gctgatacca aaagctttca
     8581 tgatttcttc tggatccgat ttatctgtaa agttgatttg ctgatctgca gaacgttcta
     8641 aaatggttaa aatcatttgt gcatcatctg atatagcttc atatgctctt ggcttcaatg
     8701 aaagattcag tacaccgtct ggtcggacac caatcactcg accttttatc tgctcgccta
     8761 gacgaggttc attatatcgt tcagaaggat ggataaaccc aagatagaaa tcttccgtta
     8821 aaacgtacgt acctgttaat ttcaagcggt atgctgttcc tatcacatct ttgtttttca
     8881 tttcttctgt tccatatttg cttaaagatt tgaatatttt ttcatctgct aaactgcccc
     8941 aaatacggtc ttttgcatca actctaaggg aaatcatcaa gcgatcccct tttttaggcc
     9001 atagctctgt cattgtcggc aattcatcaa gagatacgac aaagtcttta tctggaagac
     9061 cgatatcgac aaacgcacct aaatcttttc gtgtagcagt aacgattcca aaagcgtaat
     9121 gaccgatacg gcttttagga atttgtgtag tgatccgatt ttcatgtttt tgattttggt
     9181 aaccgaatcc ttcaactgct tcaccgattt tgtgttcacc ttctgatttg cttaagccaa
     9241 aggtttgtcc gtttttttgg acaaagtagt ttttttcatt ttcatcaata ataatacctg
     9301 taaatatagt tgctaataaa tcgttcattt ttttctccct ctatagtgaa aggttttctt
     9361 aatacatttc tttgctatca taacataatg ttatctactt gaatagctga gccttggttt
     9421 ggaaaggtga aaaaaggagt atgacaacag ttgtgtcata ctcccctttt tcgaataaac
     9481 ggtgttaaac ggcaggctca cagcctcttt catttgccta aaattaattg ctcccatttc
     9541 ctccgaacca tccagtttta cagttgatgg ttacgatgta aaatggcaaa gcactttctc
     9601 caatgccttt catcaatgca gattttgttg aagtggcata tagattataa tagccttccc
     9661 cattttcgat tctggctttt tctaatctgt aacctccttc aacgatatat ccacgttgga
     9721 tacatgtagc aatcactttg ctttggacac ctggtgccca cgtccatgct ggattagatg
     9781 catcggcaat atcacttgct ctggcagatt tgattggctg attcgctgag tttgaagagg
     9841 aaggggtcga ggatgccgct tcttgctgtg ctgcttgtgc gaccgcagct tgttgagcgg
     9901 cttgagctgc agctgctgct tgagcggctt gttttgcttg ttcttcagct gcttttttcg
     9961 cttcttctgc ttttttcttt tcctcttctt cttttttctc tttatcagtg agcatcttat
    10021 ctacttgttt caattgttca ttcaactgag cagatagatc tgtatttttc actttttcta
    10081 ctgcttcttt tgccgtcttg tattggtcgc gtgttgctga atccactact tttccgtctt
    10141 taaagactat agctgttttt tctttagctg tttgcaattc agcatattga tttttagctt
    10201 ctgtttgtgc ttgttgaatc aattttccaa aatctgtgtt atccgtttcg attgcttgga
    10261 tctctttatc tgctttcaac ttgatgtcat ctgctagatg atcatcgacg atggcaggtg
    10321 ctatgaacca ctcattcact ttttggatat ctcggatctt agattggata tcttggtaag
    10381 ttgtttctaa ctctgaatat cctttttcat tcttaagact ggataaatca ttttttagtt
    10441 ttgataaatc ttgatgaatc atatctgcac gaataaattg atggtgatca tctgtataaa
    10501 aggcagcaag tttctcatga agttcttcca tttgttgctg tctttttgct tctgctgcta
    10561 cttcggcttc tactttttga tgatggtcat aataagccca gcttcctcca ccgagtagaa
    10621 gtactgctgc taaaacgaga taaggtactt tttttgattt tttcttttgc gctttatctg
    10681 gatctgtttt ttctaagttt gattcttgat gtgttttttc agtatctggc acgattgtta
    10741 ttgatttcgc ttctgcttca aacagcttag gctggttctc ttctttttct atttttgctt
    10801 caagatcagt accctctttg cttacagatt gattttcagc atcggattca ctatttattt
    10861 catcgggctc tgttgacttt ttgtctgtgt tttcgagtat ttcttttttc tctgcttccg
    10921 gcagtttatt cgttttttcc ttttctaatt gtttttcagt aaatacttca gttttttctg
    10981 tgatatccgt ttttacgtct tttattttta ctgtttcttt acctgtggcg ttttctctat
    11041 cagagtcttt ttcagtttct tgttcctttt ctggctctac tttgttttcc tgtgcaattt
    11101 tttcactgat cattttgagc agctctttcg cgtgctcttc ttttgtatct tcacgatgtg
    11161 ctttgatata agcggatagt atcgaatttt cttcctcatt atcgacgatt tctttcagtt
    11221 cagctactct tcttttttgt tttgattcaa gcgtttcttc ttgattcttt cctgattctt
    11281 ccgaagtctc atgaacagtt gctttcttct gttcattttt ctctatatta gttacttgat
    11341 cgtgagcagg tggttcttcg tcttctggtt ctgctttttc gaataattcc attacagatt
    11401 ctagaggtaa atctttcaat tccgaccatt cgattggatc gtttaattct gtatcaggaa
    11461 agactgcatc ttcagttgat ttatcctgca tagctgcatc tgtatttgtt tcttcaattg
    11521 attcttttat ttgctgatct tctgcagtta tttgatgacc gcagtctgga cagatagtat
    11581 tctgctccat ttttttcgat ccgcaatttg gacatttttt catcacttag cgatcggctc
    11641 ctttactagg tttacgctac catttaatct atcatattct atacgtcacg ccaatagctt
    11701 atttttagga tttacttaaa aataatgaat gaatttcctt cctatagaaa aaaaagcttg
    11761 agactgcgta gtctcaagct ttt
//
LOCUS       DACDRN010000073        11716 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDRN010000073 DACDRN010000000
VERSION     DACDRN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.073"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(179..586)
                     /locus_tag="IWJ80_002282"
     CDS             complement(179..586)
                     /locus_tag="IWJ80_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP9461978.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(775..1602)
                     /locus_tag="IWJ80_002283"
     CDS             complement(775..1602)
                     /locus_tag="IWJ80_002283"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAP9461979.1"
                     /translation="MPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPII
                     QAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDI
                     RGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILN
                     TNLFNIDETSYSMGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKN
                     FPGNITRAKGFLWFAQHSDFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            1911..2129
                     /locus_tag="IWJ80_002284"
     CDS             1911..2129
                     /locus_tag="IWJ80_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9461980.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            2150..2785
                     /locus_tag="IWJ80_002285"
     CDS             2150..2785
                     /locus_tag="IWJ80_002285"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9461981.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(2978..3217)
                     /locus_tag="IWJ80_002286"
     CDS             complement(2978..3217)
                     /locus_tag="IWJ80_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461982.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(3241..4764)
                     /locus_tag="IWJ80_002287"
     CDS             complement(3241..4764)
                     /locus_tag="IWJ80_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9461983.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(4782..4904)
                     /locus_tag="IWJ80_002288"
     CDS             complement(4782..4904)
                     /locus_tag="IWJ80_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9461984.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(4934..5917)
                     /locus_tag="IWJ80_002289"
     CDS             complement(4934..5917)
                     /locus_tag="IWJ80_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9461985.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(5941..6090)
                     /gene="rpmG"
                     /locus_tag="IWJ80_002290"
     CDS             complement(5941..6090)
                     /gene="rpmG"
                     /locus_tag="IWJ80_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9461986.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(6111..6488)
                     /locus_tag="IWJ80_002291"
     CDS             complement(6111..6488)
                     /locus_tag="IWJ80_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461987.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(6520..6699)
                     /gene="rpmF"
                     /locus_tag="IWJ80_002292"
     CDS             complement(6520..6699)
                     /gene="rpmF"
                     /locus_tag="IWJ80_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9461988.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(6714..6983)
                     /gene="rpsN"
                     /locus_tag="IWJ80_002293"
     CDS             complement(6714..6983)
                     /gene="rpsN"
                     /locus_tag="IWJ80_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9461989.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(7506..9278)
                     /locus_tag="IWJ80_002294"
     CDS             complement(7506..9278)
                     /locus_tag="IWJ80_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9461990.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINM
                     VPAFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     MQIADDLSNLPLSYFSKHNLSDLSRTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(9232..10986)
                     /locus_tag="IWJ80_002295"
     CDS             complement(9232..10986)
                     /locus_tag="IWJ80_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9461991.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(11096..11665)
                     /locus_tag="IWJ80_002296"
     CDS             complement(11096..11665)
                     /locus_tag="IWJ80_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP9461992.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         3695 a         2261 c         1692 g         4068 t
ORIGIN      
        1 aaataagtaa tctaaccgac tgttaactaa accattgcag gtcccgttaa agatacttgt
       61 gcctgttgtc accgcttctt tttcttgtcc acaagcgctt aacacactta ataaagcccc
      121 tgctattatt aataataata tctttttctt attaaaaacc tcctcatcta tttccagatt
      181 agtaaatgta gtaatctttg ctttcttttt tctcaatact aataactaac ttatggggta
      241 aacaaatact cgtttgaccg ggttttgaga tccatccagt tctaaccgcg atttgatcag
      301 gactattatc ttctttaact cgaattcttc catctttaat ctcaacaata ttatattgtc
      361 ccttcgcagg atgataattc acgagtttat gattgatctt atccaaatcg aagcgatcca
      421 tttccttgcc atcaatttga acgatggcat atttgatagc atcttcagtt gtttcattct
      481 ccttttctat agagaataca aataagggaa taaaagaggg aataatcaaa atgccaataa
      541 ttatgacatc gcctatcttg acaccagctt ttcttaaaaa tttcattaac gatatctggc
      601 taaacatatt agaatttttc catggaaatg gtgaaatttt tcggaaatta tctgcattaa
      661 gagaatcagt taaacaaccc tcaagttgtt ccttaataca ttgttgatcc atcttcatac
      721 caataaaaac tagtttatta atgcgttcac caaacaacgg atgccaggct tccttcaatt
      781 cgggtttttt tgtataatca tttgacactc aattttaggt aaactagcta cccaataatt
      841 taatgattca atacttttat ggctacccga tgtttcaaag atcgtcgaaa aatcactatg
      901 ttgggcaaac caaagaaacc cttttgctct tgtaatattg ccaggaaaat ttttatccgt
      961 aagatgggca aatttttgtg cgtcaaggag taaccgattt cgatagataa aacttgagat
     1021 accatattct tctgtttcag gaatatgatt ttgatagcca agctctaact ctttgaacca
     1081 gcccattgaa taggaagtct catctatatt aaaaagattt gtatttagaa ttttcgataa
     1141 gtctacttca cctttaattg tcctaattat tttggcattg gactgtaact tagcaattag
     1201 accgttaatt ttgtaaatcg tcttggttga cactaagtca ctattattta aaagtaatat
     1261 gttacaacac tcgaattgat caattaacaa gccacgaata tcctgttctt cttcaactac
     1321 attaccttca gcttttcttt cctgaatctt ttcacccgtt tcgaaattat cccaaaaacg
     1381 ttttacatcc actactataa taatggcacc aatctctaat cgttgggtca aatcaatgtc
     1441 taaagggttt ttagctaata taattgcttg aataattgga attggtttgc tgattcctga
     1501 agcttcaata acaatttgat tcaagtcaga aaattgcact aattgatcta gttcaacaat
     1561 caaatcttcg cgtaatgtac aacaaatgca tccattcggc atcgaaacaa gtttttctcc
     1621 agttctttta aaacctttct tgtcgactaa taccccatca acatttactt cccctatgtc
     1681 atttacaatg acagcaattt cctttccgtg atcaccgtac agaatatgat ttagaagtat
     1741 cgtttttcct gaaccaagat agccagttaa gattgtaata ggtatttttt catagggaac
     1801 ctctttctat accttgaatt tacactttaa gtgcaacaca taaaaatgag tcataatcga
     1861 tatccaacta gtataagttt gtcgagaaca ctcctagaag gaaaataatt atgagccagt
     1921 attgtcatct tactatactc aaacgagaaa gaatctttct gcttcacgaa gaaggatttt
     1981 ctattagtat aattggcaac gacattcagc atagcccgtt tactatttta catgaactga
     2041 gaagaaacaa gaaaaaaact aatcaccttc caaagcggaa aaaaattatg cttatcgaag
     2101 aaaaaaatgc ggacgttatc acattctaaa tgatccgtga gtttggtcaa ttgttcgtca
     2161 tttatttgtc gattttcagt ggtcgccaga gaaaatctcg aatcgtttga agcatgaaca
     2221 tatggacttt tcaattagtt ttatcacgat ctaccgtgca atttgcaatg atcttttcga
     2281 tacagaaccc ctctcacacg gcaataaagg cctgatccgt tcattgcacc atcgaggaaa
     2341 aacaagacat acaaaaaatc atgtcgagtc tcgtgggaag atttgcctta ctcattcgat
     2401 ccatggccat ccaaaatccg cagatgatag aagtgatttt ggtcattgga aagccgatat
     2461 tgtagctggc aaaactagtg gtacttgttt agtgactttg acagacagaa aatcatgctt
     2521 ccttttagca ggaaagaagg tccccagaaa gaaagccgct tatgtgagcg agaaaatgat
     2581 tgaactttta agcagtattc caaatgaggc ggtcaaaacg atcactccag atagaggaaa
     2641 agaattctcc aatcatttcg cagttacaga agtattgaat aatatgccct tttattttcc
     2701 agatcctcat gcccttggca acaatgaaca aattaaaata cgaatagact cattcgagaa
     2761 tacttgccaa aatcgaaaga gatagctacg gtagataact caacgataaa tcagatgatt
     2821 gaaaaactga ataacagacc tagaaagtgt ttagattgga gaacacctta tgaagtcttc
     2881 tttgataaag tgttgcgctt gatttgacaa ttcaagaaat aataaaaagc agatcaaatc
     2941 caaataaggg aaaaatgatc tactctggta ttatcaatta attggccaag ctattatgcc
     3001 gttttgctaa acgtctactt cttccagtat tgaccttgat aaaacaacaa tcaggatccc
     3061 gttttaatac tctatgaact gtgtcaatac taacatgtaa agttgcagca atatcagcta
     3121 atgtactccc atttaatctc atttgctttg caaattgata acgccgataa gcatcaatat
     3181 tcttggaatc catagacaat aatacttttc ttaccatgta tttcctccaa agattggatt
     3241 ttaatgggcc atcatttctt gagcaatctc atgctcagat aatgaagctg gatagaaagt
     3301 aggccaatta tgcatttcat tataaagttt ttcttgactt tctccaccaa agaaaatatg
     3361 gaaatgctct gccttacttg gtgcaattcc atgatcgcta aattgaatat atttaaaacg
     3421 accagcacct gcatcatcag tttcaaaatt aaaacgaaca ccacggtttc ctttttcata
     3481 attcaaaatt ttgtatccaa catatttgta tgtgtattga tgatgttcac cgttgactac
     3541 aaaatcaatg gtgttgtctt taatgttgat agtatcaata tctgtcttat agccagtagt
     3601 ataataatct ttgtactcgg cagctgtcat gtctttattg agttttgctt tgtaatcgaa
     3661 aacttcatcc aaaattccat cttttaacaa cggataaaca gattgccatt cgcctgtata
     3721 atcactcaat tcgcgatcct tcacatcggc atctttaaag tacccattag caactgtctt
     3781 ttcatccttc gcttcttttt ctggaactac ctcttcacca gcaaccatag ttgttttctc
     3841 taacgctttt agattgtctt ccattactga aacataatct tcaccattat ccatttgttc
     3901 ttttgtaaga ctttcaagag gattcaatac ttccaatttt atcccagctt cattggccaa
     3961 agtttttgcg attttatcat tagcattctt ttcaaagtaa atataattga tcttattttt
     4021 tttaacatac tcttttaatt cagctaatcg accagaagat ggctcttctt caggacttag
     4081 tccagcaata ggaacttgta tcaatccata atccaaagct aaatatccaa aggctgcatg
     4141 ttgtgtcaca aaactttttt gtttcgcttc tttaagacta gtggtatagt cagcatccaa
     4201 ccgtttcagt ttagtcaaat acttttcagc atttgtctca aatacttttg cttttttcgg
     4261 atacaattta actaactgat ccttgatatt tgaaacttcc ttaattgcca ttttgggtga
     4321 aacccacgta tgaggatcta gttcatgatg gtgatcttct tctccgtgat catgatcttc
     4381 ttcttcacta cctggcaata aaatcatgtc ttttgttcct tctactacat taggcccagc
     4441 tttcttccaa ctttctttag cttttggtac ccatgattcc atgttttcgt tatgatatac
     4501 aaaaacatca gcatcatgag cttttgccat atccttagct gagggctcat agtcatgagg
     4561 ttctgatccc gctggaacca ttaaatccac gttcgcttca tcgccgacga tgtttttggt
     4621 gaaatcatac attggataga atgttgttac aatttctaat tttccatctt tatcattcga
     4681 tacttttgca tcattagtgc tggagcaagc tcccaataat cctagagctg ctatcccaat
     4741 aagtcctaca ctgattttct tcattctttt tccttcctta attatgctaa tttcttcatt
     4801 ttattttgtt tatgttcttt gataattttt aatgcccttt ttctagtttt gatattcggg
     4861 cttttaagtc gtcgatatgc actagatata tccgttttct ccattagaaa ttacccctaa
     4921 tcaatactta tcattatttg ctttccgcca gtaatttatc aaaactctgt ttgatccttt
     4981 cttcatccaa catttttcca attagaataa tagtggtttg gtctttttgt cgagtggtgt
     5041 attgaaaatt aacctgttcg tttacccctt gtatggcaac tataaaatct tgatcttgca
     5101 aagcaatcaa tcctttaaaa cgatacaggc tcccttggtt ggttaaaatc agccggtcaa
     5161 ttaattggaa taaaaaatct ttttttatgt cttgatctga agttaagaga attgatttaa
     5221 actcatgttc ttgatgatgt tgatgatagt gtttgccatt atagtgattt tctgatgaat
     5281 cactttcaaa aactaaggga tgattgaatt tttccaaatt ataaaaatcc tttttttgaa
     5341 tcgtttcact atgtgtgaac attcgaatat cggataaagg gttaatactt ttgatttctc
     5401 tctgtaattt ttttagtgca gataaattca agttttgttt tctagacaca aaaatgcgat
     5461 ctgctatgac aagttgctta agggcttgtg gttctctttc aataagttca aaactgtcac
     5521 tatcaataac agtcaataaa ctatcgatat atactcttcc tcttagttga ggcgctgtta
     5581 aaagtgtttg aatgattggt tggggatctg ctatacctgt agtttcaatg atgatttgtg
     5641 aaattggttg ttgatgttct tctattgttt ccagtaaaga aatcaatatt gctaataaat
     5701 cactgcgaag actgcaacac atacatccat tatttagttg taacacccgt tcctcgctat
     5761 gaactaataa tttgtgatca atcccaactt caccaaactc attttcaatt ataataattt
     5821 cttccctggg gtagggtgag acttgcaagg cttgattgat tagcgttgtt tttccagccc
     5881 ctaaaaaccc agaaacgatt gtaactggta tgcccatttt gtcacctttt taccacttag
     5941 ctatttcact tctttgaaaa ttgcccgtct gcgcaatttt ggcgaatact tttttaattc
     6001 taaccgttca gggttattcc gtttattttt gctggtgaga tacagacgtt ctccagtttc
     6061 gacacattcc aaaataatat tttgacgcat aaatcttcct ccttcaatac ttatcgatct
     6121 tcaagatatg cttgaatttg ttgtgcatct tcaaaagtta actcgccaga ttccaattgt
     6181 gaaactaaat tttctttcat atcaacaggt atctcccaat aaccatcaat acattgaaat
     6241 aactcctcgt catactcttc aaaaattgga tagattctta aaaaatcatc aaatgataag
     6301 ccttggtcct catagtaatc tttgactaat ccattttcaa ttgcctgttg ggtcggtaaa
     6361 cccgtttcag taataattcc tctctctacc atcgaacgat aaaaagtgca tttatctagc
     6421 gattgctggg tcgtttcaag ataatacttt aatccttctt caactaaagg tgggtaaata
     6481 atttccattt tgattccctc ctaaaagtta tatattaaac tattttgaag ctcctttaat
     6541 tactttcttt ccattatagt acccttttaa agatacatga tggctacgtc tataatcacc
     6601 attcgcttcg tcaaatgaaa tttctggttt tactacagtt tgatgagttc ttcttaaccg
     6661 tttcttcgcc ttagatgttt ttcttgctgg tactgccatg atatttcctc cttttaccaa
     6721 cttgcttttt ttactccagg aatcaaccct tgatgggcaa gttctctaaa cttaatacgg
     6781 gacataccga actttcgcat ataccctctt ggacgaccat ccgtctgatc acgaagcctc
     6841 agacgattcg gatttgaatc tcttggcaat ttagcgagag cggtgcgatc tcctgcggct
     6901 tttaattcct gacgtttatc tgcgtacttt tcaatcatcg ccatttgttt ctttgcttta
     6961 gcaattttcg acttttttgc catatacctt tatactcctt ttattttgat ttagacatct
     7021 caaatcgtaa tcgttacgtt ttgacactga tactttacca acaagagttg ctcttgtcaa
     7081 caataaataa catgaaataa aactcatatg aatagataac tatttgaaaa cgaatttatc
     7141 ggtacataga gataatcaaa tcatttaaaa gaatatgcag tttaagctct gaaaaataag
     7201 aattcacttt tattagtcca atcaaacaca aataccgttt taagtagctt ttcctctact
     7261 taaaacggca atatgaaaga aactataact ttttattcaa tcttttatgt ttaactggtt
     7321 tatcgccaat tttttaaaac tttgattatt ccttatatta taagaataga cattaagtga
     7381 actaattcaa tcatactgat tctttcgtgt attcaaaatt agatgctgtt taataaaatt
     7441 attctttttg aaaaagaatt ataactatat cccaaaaatt gtaggttaat ttaaagccta
     7501 tttcatcatc gtgattgagc ttctttttca tagacgaatt tctcagatag tttagttttc
     7561 tttatgagat ttttgtatat ttctgacctt cgatacaact cactatgagt accttgatca
     7621 actatttttc cttgatccat cactacaatt ttatttacct tttcaataga cttcaagcga
     7681 tgcgatataa ttagtactgt cttatcttgt attaaacgat ttaaactttc ctgaatacgt
     7741 ttttcatttt ccgcatctaa actggcagta atttcatcta aaattaaaat tggcgcattt
     7801 ttcaaaaaag cccgtgctat ggataggcgt tgtcgctctc ctccagaaag tgaacttccg
     7861 ttttctccga ccaatgtatg ataaccgtca ggtagttttt caataaagtc ttcgcagtta
     7921 gctaaacgag ctgctttttt cacttcctca tctgacgctt gactatttcc aatacgtata
     7981 ttttccaaaa tacttgtatt aaacaaagtt acctcttgaa aaacaatagc gattttgcta
     8041 aacaaagaag ctggagatac acgtttaata tcatacccat caattaaaat acagccttca
     8101 tcataatcat acagtctaga cactaatttc aagatactag ttttcccgga accactagca
     8161 cctactaacg cagtcacttc tccttgttta gccgtaaaag agatatgatc taaaattggg
     8221 gtattgttat catatgagaa agagacatct cgcaactcaa tgtcaaaaga tttcagagga
     8281 ctatctgatc cctcttggat actggtttcc ttcatagctc taattcgctg tattttagga
     8341 gctaaataaa atatttcaag cactgcttct ttcatagaat cgaaggaatc ctttattttt
     8401 attgcggcta gtaagtaacc aacgacgtag agtatagtca cttcacccgt taacagtaag
     8461 tgaacaccta ctagaatgac tactgctaaa ctaacatatg agaaaataga ggataaggcc
     8521 atgactgaaa aagtacttaa ttctaccttt aaatgaatcc tttcactctc ttccattttt
     8581 ttgaaaagtc tgtcttgaac ttttttagat agattaaagc tattaatctc ttgctgcaat
     8641 tctatagttt cttgaaattc ttccgagttt tctctcaaag tatcgtaata tttagtattg
     8701 gctttcgttt gggatttctt tgataacaag attaagacaa aactaaataa cgttggcaaa
     8761 ataacagcta atcccatttt gacattacca accaaaagca tcactgaaat aaaagggaaa
     8821 aacagagcca taccgccggc tttaggtatt gcatgactca tcgcatgctc aataccttca
     8881 acgtcagaca tgatagttcg agataaatct gataaattat gttttgaaaa ataggataat
     8941 ggtaaatttg acaaatcgtc tgcaatttgc attcttaaat gcgcactttc tttataagta
     9001 ctattatata agttttcata ctccctatct aacaaccaat acatgaaaag caaagtaacg
     9061 aacgatactg taaaataaag ccaaggcggc tttacatttt ctaaaactaa ttcgtctatc
     9121 agcatcatta taatgaatgc tggtaccata ttaatgcagt atacaaaaaa gctagaaata
     9181 gatgctttaa ccaaatcttc tgcgccctga ttagttaaag cgaatttgtt tttataaaaa
     9241 cttttcattg tcaagcctcc aatcatttgc attctcataa ttagtgacca attgtttata
     9301 cacttgatgt ttagccatta attcattatg atttccacgt tctactattt tcccttctga
     9361 caagacaaga atttcatcta cagatttaat gctggataat cgatgagcta tcataataac
     9421 tgttttttct ttcatcagat gttgaaaagc cttttgtagt tcatactcat tatctggatc
     9481 aatagatgca ctggcttcat ccataataac aatctttgca tcttttaaaa ttgctctggc
     9541 aatagctata cgttgttttt ctcctcctga cagataaact cctttactac caattaaggt
     9601 atgctctctt tcgggaaatt tttctaaaat cgattcgcaa ccggctaatc ttagcgcgtc
     9661 aaatacttta cttgctggcg cttgaggatt tgctatagaa acgttatcgt aaatactctg
     9721 attgaatagt ttcgtatctt gaaagacaaa agcaattgtg ttaataattg tgtcttttga
     9781 atagtgattt aaaggcttac caccaatttt aatctgtccc ttatccacat tgtagaaacc
     9841 tgaaattagt ttagctatcg tactctttcc gcttcctgat tttcctacta atgcataaat
     9901 tcttttttgt tctaaaacta agttgaaatt ttctaaaaca cactcttctt tgtatgaaaa
     9961 gctgacattt tcaaatgcaa tgtcacaatt tttaatctgt gtctcgttcc catattttag
    10021 ttggtcttgc ttcatttcta aataaaggga gtctagtgtt tcgactgcat atcttgcttc
    10081 aaacaagtgc atacttacat acattattct catgaaagaa acaaaaataa ttccacttaa
    10141 aaaaaacaac atcaacaagt caacagctaa tatcttggga gtggtaagag aagttataaa
    10201 aaaggttata ggaactatca aaatgcaaac tatgccaaaa aatagccatt gaaaaagaac
    10261 aaaaggtgtt ttcccactca aagaatactt atatgcaaac ttagcataat cgttaattgc
    10321 tttattcaag gctttaaatg atgttacttt agcaccaaat atctttataa cttgaattcc
    10381 tcggatatat tctaccgttt cagagcttaa cacatctaac gattcttgat atttttgaat
    10441 aaaatttgta ttctctgtca ttttctttaa caagaataca ccaattacta acatggcgat
    10501 tagaaccaat ccaattttaa aattaatgaa aaagcctaag gcaactgata aaacaggtgt
    10561 tactatggct tgagcactat ctggaatcaa gtgagcaatt gccatatgtg ttttagccgc
    10621 gttgtcatca attgtctttc gaatatatcc cgaagaattt aaatcaaaaa aacgaaaact
    10681 tgcttctgtt aaaccgctaa ttcccttttt tctcaaatta gtctctaagc gaaacccaag
    10741 gatatgagaa aataagccag aaaaaatata aaataaagct cctaaagtta accaagcaac
    10801 agtttgaacc gcataactct tagctaaata ttcatctcct acaataatta gagacttaag
    10861 aaatttgtaa atactgtaat atccatagac tgttaatacg acagaaaaat cagaaaataa
    10921 agtggctaac catccgtagt atttcatttt agggacataa ctaaataatt ttttgtatac
    10981 tttcattttt tcctcccccg cattcaattt cttctcttat tcgtttacaa ctgttaatgc
    11041 tttccgtaat agcgtaaatt agagatgtac aaacagatag aaggtaaact acgttctatt
    11101 ttttgtattt ttttaagact tttcttccaa tcaatacacc aatcatagat aaacttgcta
    11161 caactaacac tgaaatgatt ccccatatac ctaccgcata gtggatcatt gcatccattg
    11221 ttgcagcaga ttgtccttta tctatccaat cttgacgaaa acgatctgta aagaaccata
    11281 taggaatcca ctgtccggca gcactgccta attgcattag agcccaagac gcaacttgag
    11341 gaattgctcg atcatatcca aatacagata gaataatatc agctaagatc gccatactaa
    11401 tcgaactgat aaaccaaggt aagtacgccc cgcccattaa cgtaaaaaat aacaataaaa
    11461 caattgtgta aatagtaaaa attccttttc tagggtagtt atagctaata taaacaaata
    11521 tgattccaga taataatcca aaaatccctg gggatatgct gtgaccaaac gccccaccaa
    11581 tactactaac cattattgaa aaaaagtaaa ttactaggga aataactgta gctaaaccaa
    11641 taaaaatata atcttttgtt ttcatatata ttctcctcaa ttctatttcg ttatgtcatt
    11701 aaaaatctgt tctatc
//
LOCUS       DACDRN010000074        11652 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDRN010000074 DACDRN010000000
VERSION     DACDRN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.074"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(148..1278)
                     /locus_tag="IWJ80_002297"
     CDS             complement(148..1278)
                     /locus_tag="IWJ80_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9461993.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            complement(1819..2025)
                     /locus_tag="IWJ80_002298"
     CDS             complement(1819..2025)
                     /locus_tag="IWJ80_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461994.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(2041..2655)
                     /locus_tag="IWJ80_002299"
     CDS             complement(2041..2655)
                     /locus_tag="IWJ80_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP9461995.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(2802..3485)
                     /locus_tag="IWJ80_002300"
     CDS             complement(2802..3485)
                     /locus_tag="IWJ80_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9461996.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(3594..4052)
                     /locus_tag="IWJ80_002301"
     CDS             complement(3594..4052)
                     /locus_tag="IWJ80_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP9461997.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(4136..5899)
                     /locus_tag="IWJ80_002302"
     CDS             complement(4136..5899)
                     /locus_tag="IWJ80_002302"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP9461998.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(6120..7580)
                     /locus_tag="IWJ80_002303"
     CDS             complement(6120..7580)
                     /locus_tag="IWJ80_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9461999.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(7788..8714)
                     /gene="glsA"
                     /locus_tag="IWJ80_002304"
     CDS             complement(7788..8714)
                     /gene="glsA"
                     /locus_tag="IWJ80_002304"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP9462000.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(8737..10164)
                     /locus_tag="IWJ80_002305"
     CDS             complement(8737..10164)
                     /locus_tag="IWJ80_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9462001.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(10195..11409)
                     /locus_tag="IWJ80_002306"
     CDS             complement(10195..11409)
                     /locus_tag="IWJ80_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP9462002.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         3534 a         2409 c         1922 g         3787 t
ORIGIN      
        1 taggaaaaaa ttaactagca ccacgggtat aatatgattt tttgagatac tttcattgcg
       61 aattcctctt tctaaaagaa cctctcgctt gaaaaataaa agattgtact ttatgataat
      121 tcaaactgag agtttctcag tttctcttta ttgtttttca ggtcgataga gcgccaactc
      181 ttcggcctgt atctttgaag aaatattcaa cgtgattccg attcctaaag aaaggatcag
      241 ataacttgtt cctccgtagc taacaaatgg caatggcaca ccagtcattg gcatcagccc
      301 cgcgatactt cccacgttca ttatcgtctg aacgaatagc aaagttccga atcctaaaag
      361 aaacaatcca gcttgctgat ttttacagcg actgcttaaa caaaagattc tcatgcataa
      421 tgagaacaat aagaaaagca cacacaatgc accgattagc ccaagctctt ctgtaataat
      481 cgaaaaaata aaatctgttt ctgtctctgg aagataccct ttttttgtga tactatttcc
      541 taatccccgc ccaaatatgc caccattata tagtgcataa aacgaatggg tcatttgata
      601 gcccgcacca tgactatcga taaaagaatc tcttaaagtc gcgatccttt cataagcatg
      661 attaaacatc tgtggaagcc aacctttatt tcctaaatat aataccccgc ctgctgctcc
      721 aatcagcaat gcagaaaaag taacgattag atagatccct tttttaaatg gaaccgccgc
      781 tgcccaaaat atgacccacg caatcgaaag gatcatcaat gctccagcaa tctttggctg
      841 aaataaaatc agaaaagtta tacctactgt aatcaggaat ggttttttta gatcttttgg
      901 gctactattt ccgtcacgaa aaaaccagct taaataaaaa atcaaaaaaa gatttgccag
      961 ttcagaaggc tggaattgaa tgccaaacag agagatccaa cgttgtgcgc cgttgacagt
     1021 gacaccgaat atccctattc ttactaatac taaaaagaaa atcgataagg ctaaaccgta
     1081 tcctgctatt ttagggtgaa gtaaatagtg tagtttgact gaacgagcta agaagatcac
     1141 gctccaactc aaaaatatga aaattagttg acgcaataat agactttttg tattttcatc
     1201 ggcttgcaat aaacgataag aactggcact gtatacttct aaaagaccga tcatggacaa
     1261 agtcaaataa ggacccaaaa tccaccaatc gatctttttt caccgcatct cctcctcaca
     1321 cagcaatcca cttgtcaggt gaggagcgag tttgtctcat caaataagtc agtcacaaaa
     1381 atataaagaa aaggtcagaa accttttcga acaagaattc tgacctagca tcatctcatc
     1441 gtccgagttt tagcactata caacgtaaag aaaacattct tagctattgg aaaaagcctt
     1501 aatccaacag tttctgtttt acccattggc gactttcgac gtttctgggc aacagcctat
     1561 gtttgtatag gagcctcacc taacaaggtt attactatat tctatttacg agttcatgat
     1621 agacttttcc taatgattag gcaatggaat ttgaaatgaa atagtgaaat ttaacaatct
     1681 ttaatatttt ttagaaaaaa ataacaaata agttgaaaga atcccgttgc aaattctttc
     1741 agttgcacca ggattctttg ataatttttt agttttatgt gtttttttat atgttttttc
     1801 acttttcgga ataaatattt atttgttaaa gtaattaaac aatccgctaa tcgtttgatc
     1861 tggcatttct atttccttac catctaaagt aacagtagta acgacaaaat ggtcttgcaa
     1921 tggcggaact tctaagtact gcccttgcag aaaatcattc actgtttcat attctttttt
     1981 catttctttt gaatctgtcg atggtttata gcttattttt aacataacta gtgctccttt
     2041 ttattcggga cgtgcaatcg tgaaaatttc tcctattgct gcataatcat ttcctgccaa
     2101 acggcttaca gcattcaatt ttcttggatc gatccttcct gtcttatcat catagactgt
     2161 ttcatttaaa tgatatttct gtacttctag caaaaataaa tcagctgtta ctttattttt
     2221 attccgtatc tcgacatgtt catataagga tgtttcaaaa cgaacttttg cttctgtcaa
     2281 tgctggtact gaaatgatta ctgaatcagt taaagtcaag tcagtcaggt acagttcgct
     2341 ttcctcatta ggcagattgg ctgctgtttt atttacttct tctacgttgt cctcatcaag
     2401 gatatggata acagcttctc ttgtctcaag aatattcttt gctgtgtctt ttactgcatc
     2461 ttgttttctc tggatcgcca aggataagat gggtcgattc gaactgacga tattgaagta
     2521 actgaagggt gcgatattca ctatatcttt tgctgattta gtcgttacta ctgctactgg
     2581 tctagggatg actgaaccaa tcagcaactt gtaattttca cgttctgtca attctgaagg
     2641 actccgttca aacatggttt cgccttcttt ctataaaatt cgttaaaaag cagatcttga
     2701 agacaaaatg aaaagtaatg cttttttcat cttcttagaa tctgctttaa actatctatc
     2761 aatttttctt ttttgtcaag aatctgtgcc gtgcgtgttt tttagttatt tgctttactt
     2821 gcggcttcga catatctttt agtgatcaat tgcggacggg tgataataga tccaactacg
     2881 actgaaaaag cacctaattc atatgctttc tctacttgtt caggagagtc agtatgtcct
     2941 tccataatta ctgggatatc gacttgttct atgatttgat tcaataaatc aaaatcaggt
     3001 gtctgcgtct ttcgtgaata ttctgtatag cctgctaaag tcgtagacaa aagatcaaaa
     3061 ccatcttctt gcgcttgaag cgcttcctca aaagtcgaaa tatctgccat agctaaccga
     3121 ccgtgttcgt gaatcatttg gacaagttca gcagttgttt tttctgtcgg acgtttacga
     3181 gaagttgcat ccagtgcaat gatttctgct tgcgtctcca aaagttcttc cacttctttt
     3241 tctgtcgcag taataaacac ttctgaccca tcgtaatctc ttttgataat ccctattacc
     3301 ggtaaatcaa cgattcgctt gatttcattg atatcttctg tcgtatttgc tcgaatacca
     3361 actgctccgc cttcttttgc agccaaagcc attctcccca taataaaaga actgtgtagc
     3421 ggctcgtttt ccaaagcttg acaagaaacg acgactccgc ctttgatttg ttccagtgta
     3481 tccacaatat ctcctccagt aatgttatat aaaaatacag cttcatataa aaatgtaacc
     3541 cttttctatt caggagacaa taatgagcta tttctttcag cagacctttc ttactaaaag
     3601 aaaattactt caaattcttc gtatacgacc agcttctctt ctgtaaattc tatgttcaac
     3661 gcttctttat aagcatgttt aaaaaactca gcatggactt tccgccaata agctaatgtc
     3721 cgatcccctt ctccttcttt ccaagcatgc tctttcgtga cttcactaaa aggcaagatg
     3781 gtcacattta tatttttgat gatacaaact ggttcatttt ttccattcag gaccacgtct
     3841 aattgaccaa cttttggtaa aggctcattc tcttgtttat acagatcgta accacttgat
     3901 gtaccagtct ttatcttttt tacaactagt tcgctcaact cgtctgccat tttttctgta
     3961 tccccaaatg aaaagaaacc taatttttct gattgataac taggatattt ttcttggaac
     4021 tttttgacat attcttctac ttgctgatac ataacatcca cccctttgct ttttataatg
     4081 aaaaaaccat ccagatacta gtataaacaa ttatctggat ggtgagcata atgaattatt
     4141 gaatattcaa gacacgcaga ctatcttcaa cgatttgttt tggtgttaaa tgataacgtt
     4201 cataaagctg ccctacagga acgctatccg tgaattcttt ctctgcacca aaatttaata
     4261 cgttcattga agaatcacca taataacgac tgatattttc gccaaatcct cctgacaaac
     4321 taccatcttc taatgtgatg actaattcgt gtgtttcttt taatttatct aatgtttcta
     4381 cgtcattttc tgtgatcgtt cctggattga tcaacgttgc ttcgattcct ttttttctaa
     4441 aagcttttac tgtttctttt cctaattcaa gaaaaccgcc taaagctaaa attgctactt
     4501 ttcctccatt atttactgtt tgataggctg gaatactata atcatttgcg atagctgtac
     4561 catgttctac accatgtgta ggaattcgga ttgctacagg ctgcgctgtt tgttcgaacg
     4621 cccaatctag catagagatc atatcctctt ttgttgctgg agcaagatat ttgatgttcg
     4681 ggatacttga taagtaagga atgtcaaatg ttccttggtg tgttcggtca ctagctgaga
     4741 tcgttccgcc accgactaaa ataatcactg gattctcgtt gatcgctaaa tcatgtgata
     4801 attgatcata tgcccgttgc aagaaagtac ttgattggaa gacgactgga cgtactccct
     4861 gagaagccaa agcagaagcg aaagtgatag agtgctgttc agcgatcccc acgtcaaaat
     4921 aatgatctgg atgtttttct tggaatgttt tcagtccaaa ggcgcctggt atcgctgcat
     4981 tgactgcggc aataggagct gtacttgttt gcattttctt atccaaataa tccatgatga
     5041 tcatattaaa tgtttctcca gaagttactt gctttgcctc cccggtggac agatcaaatg
     5101 gtacatgcca atgatatttc attttattat cgattgccgg ctgataaccg cgaccttttt
     5161 ccgtatggac gtgaagtaca atcggatgat caatatcttt tacttcttca aataaacgga
     5221 tcaccgtttc cagatcgttt ccattatcca cgtaacgata atcaaatccc attgctttaa
     5281 ataggttatt gctgctttta ccgttggttt gacgaagctc agctaagttt ttatataaac
     5341 tgccatgatt ctcatcaata gacatctcat tatcgtttac taatacgatt agattggatt
     5401 gtaaaacacc accattgttc aatccttcaa aagctaagcc accacttagg gagccgtcac
     5461 cgataaatgc taccacattt cctgattctt tcttcagatc tctcgcttta gctaaacctg
     5521 ttgctaagct gatagatgta gatgtgtgcc ccactgtaaa atagtcatgt ttactttcat
     5581 gttggttgga atatccgctc acttcacggt aatgtccttt ttcaaaacca ttttttcgac
     5641 cagttaaaag cttatgacca taagactgat gggaaacatc ccaaacgatt ttatcctttg
     5701 gtgaatcaaa tacataatga aacgcaatcg ttaattctac tacacctaga ttcggaccaa
     5761 cgtgcccacc tacatcgctg tccttttgca agattttttg gcgcatctct tctgccaact
     5821 gtttcatttc ctcaacagat aattttttga catctgcggg gctattgatt gcatccaata
     5881 catgtaatac tgtactcata atacattctc tcctccgtac tatcttcttt taatgaaaat
     5941 agatttgtta cactttatct attctactac ttagagttga ctctaagtca atagcatttt
     6001 aaaaaaattt cagagaaagc ttgttatata agtagatctt cgcacgaatc aggactcaaa
     6061 taaaaacctt ctacagacaa ttaaaaagct ttgtagaagg tctcctaatt aaggtctcat
     6121 caagaaatat gtttttttaa taggtgaagt tcctgagcaa tatgatacaa atggatgttt
     6181 gcacctttta atgagattgg atagatcttt gtcttttcag tttctaatcc attttgatag
     6241 ctagcagcat cgcacaaaac cactcttttt ttcgcacata gacattcatc aggcaattct
     6301 tctaatgaac gaaataaaat aggatttatt tcagcgatgc cttcttccgc atacaaatga
     6361 aattgggttc gataaaaaaa ttgtttgact gaactttctg aaacaacaaa aatttctatt
     6421 tcctgtctcc ccgcttgttc actgttgatc aaagaaagac ttttgacgaa ttttcgaatg
     6481 aaattttcat tccatcttag ttggatttga tttttctttt tccactcttc caatatttct
     6541 acaatttttc cgtacagaag ttgttctttt tctgatagaa gatagatcct ttccggtaaa
     6601 aatacttgtc catctgccca caacgtacgg acaaattgta aaaaggattg cttgaaaata
     6661 aaattttctg catcgtcgat ttcagcgata ctaataatct gtaaaatcaa ttcattcagt
     6721 gataggtcat tgagaacaaa atttttataa acaacttcga aatcttttct taacagctcc
     6781 atgttctcat tggtataatt tctactgttg aacaaagaaa aaatataata gacatcttct
     6841 tcttgtaagt ctagaattcc tttttgctct tccaatcctt gacagactaa ttgaaataac
     6901 ggcttcgttt ctgcgctttt cttatcttct tctttcagtc caaggggaaa tgcctttctt
     6961 ccgattgcga tctctattcc tcgtatgata aaggactttt catcttccga atatcttctt
     7021 ttaaaaaaat gagtctctac ataatcaatc aactcgttac aagcttcttc tacatacgga
     7081 tttttatttt catagccttc ccaattgaca taacgtacta aagctagcaa gatgttccgc
     7141 gagtcaaatt ctgggataat gatttcttta tttttcaaat aatcattttc tttaaaaaaa
     7201 tccaatacga tttttttgat tgtatatact ttgctcaaag aaataaattc aagatcagcc
     7261 agttctgtac tcgtgaatat accattaaaa taataactta aaaactttaa aaaataagat
     7321 tgaccgtaca gaaactgtgt gagctcagaa agagcaatac catgtttgaa tacgagttta
     7381 tacttatgct tctctcttac ttgttctttt atacaatatt ctagacactc ggctagactt
     7441 tctagatcgt gaaggatcgt tggtgtagtc acccttagag aaatagctgt ttgttcgatg
     7501 tctatttctt ggaattcaaa taattgttca cacagataca cttttctaaa tatatttcgc
     7561 tcgatgtaat tttccagcat tcctgataca cgcctccagt ttttcttatg attttattta
     7621 atcatatctg taaatcgtat ttttgtttcc tttttattta gaaaagccct ttcaaaaacc
     7681 aataaaaaaa gaaactggga caaaagtcaa acaagctcac aatttcagcc tcattgtagg
     7741 tagctttgag ccgtttggac ttttacccca gcctctttca agaatggtta ttcaaagatg
     7801 tcagcgtcta gttctttgac tacatctttc aacaattgga taccagctgc gctgttacca
     7861 aatggatcta gtgccgggct gaagacacct atgccgtaac gatttggcac agctgccatc
     7921 aacccgccgc ctacaccgct ttttgcaggt acaccaacat ggacggaaaa ctctcctgat
     7981 tcatcataca aaccagcagt tgtcataatt gatttgacga ttgtagcact ttcttcagta
     8041 atgatttgtt tgccattcca aggagcaatt cctttatttg ccagcacagc agctaatttt
     8101 gctatgcctg tagcggtgac attgagggag cattgtctga aataagcatc gagtatctcc
     8161 ggtacgtctc cttcgatcat ttgattacct ttcatatagt aagcaagaga gcgattgata
     8221 tcacctgttc ttgattcaga atgatagatt tcttcattca atgtgatatt aggatcatca
     8281 cagatctctt tcataaatga caagattcgc tcaaattttt catctgcatc tttaccggca
     8341 atcaacgagg ttgttgcaat cgctcctgca ttaacaaaag gattcattgg atgtttgcga
     8401 tgattgattt ccatattcat gatagaattg aaggcaaaac cggttggttc cgtgttgatt
     8461 gtttgaaaga ctttttctat tccgttgtct ttgattgcta ataacaatac gggaactttt
     8521 gacatgcttt cgatggcaaa gcgcacatcg gcgtctcctc cctcaatcag ttgttttgta
     8581 tccaaatcat aaatcgccat acctaattgt ttgggatcca cttcagctaa tgctggaatg
     8641 taatccgcca cttgtcctgt ttgataatag tggaaatttt ttccaatcaa ttgttctaat
     8701 tcttttactt ccatttgaat aaccagcttt cattttttat ttttctgcat gctggaattt
     8761 atgacggcat gcgtagatcg taaatggaat aactaagacg ataagataag aaacaatcag
     8821 aatcgtttca taaatcccat gctgtccagc tgcgatacta gcaggtggaa caaatgagat
     8881 acaaaaagca aaaagtgaag tcaaaagacc taaacaagca acgattactt ttacaacttt
     8941 tccgcctgga acctgatagg ctctttcaag actgtcatct ttaaaaacca gcttcaaata
     9001 gcccgcgaaa aacataaagt acaacatcag gtagattaca acagtcaatg acatcgctgc
     9061 aagaaatgaa agattattcc ccccgccgct taatgtaaag acggctgccc agattgttgc
     9121 aacgatccca tttgcgatca tcaaatgagc aggaacaccg tgtgtgtttt cttttgcaaa
     9181 atattcagga agtaatcctt cttctgctgc tacgtgaagt gcttttgaag gagaggttac
     9241 ccaagaagca atttctccga tgacacctaa acaaacaaga atcgcaagaa ttttcgcaat
     9301 ccatgcaaga tttggatcaa tgtgtcttaa taggaattct aacgattgaa tcacccctgt
     9361 attaagagac aaaccatgtt gaggtactgt agtagcgacg gtcaatccgc caaaagtatc
     9421 taaaatgatt gccagaataa ccaatacaaa cattgctaat ggataatcct ttttagggtt
     9481 ttccatttca tttgcatgac tggcagatgt ttctacgccc atatatgcta aaataaagga
     9541 aacaaaaaca accagcgtag gcaatttcga aaaatcagga atcaatgttt tcatagacag
     9601 tatcgtttgt agtggagcgc ctgaagcgac ataatggatc cctaagccaa acaagactaa
     9661 tgctggcaac aagataccag ctacaaaacc gatccgagca atcaatgaag tgtatttggt
     9721 tccttttaac tgtgagagta aaatagccca gaaaataatc aataggattg ccaatttcag
     9781 atatttattg ttttgtatag caccaatacc cgttgcatag gataatgcac cagttaagaa
     9841 atacagcatg gtcaagtagc caactgttat ctcaaaccat tggaaaaaga ttgcggcaaa
     9901 accgaatcgt tttcccaatg tgcgactgac ccaagtatag acaccgcctt tttcccatcc
     9961 tttcaccgtt gccatctctg ctgcgcacaa tgccacaggg ataaaccata gcaaaccgcc
    10021 taataacaag aaaaatacta agctgaagcc tgaggtcgcg aatgtgggat attcatataa
    10081 tgaaacgacc attgagatgg tcatagccaa aaagccaaag aacgttaatt tttttgcagg
    10141 aattttcgtt ttgctttgtt gcatgaatct tcctcttttc tatttgaatt tgtttcaatt
    10201 gagtttgttg ccaaagaaga aaaagtccaa tccttcttct tgttcttctt ttgtagtagt
    10261 gatcgttgta aagtgcatcg ccgattttaa aatcactgtt gtcagcacat aggcatacac
    10321 ggcagtaatg atgacaccaa tcacttgaat ccctaagaag taccagctgc ttggattcgt
    10381 tagtaaatgg ctgttagatg caaagatccc tatcagaatc gtacctgtga atccgcccat
    10441 gccatgaaca ccccacacat caagtgcatc atcccattgt ttctttttac gaaaatcaac
    10501 agcagcatga cagacgatac ccgcaatgat cccaatcagc atggcacttt ttgcttctac
    10561 ataaccggca caaggcgtaa tggctgccaa tccagcaacg gatcctgtta gtacgtcgac
    10621 aaaatcaaag ccatttcctt ttacttttgc aatgatcagc caagtaatca tcgcaaatgc
    10681 aagagcgatg aatgttgaac caaatgcatt gactgcttgt tcatctgctc gaagtgcacc
    10741 accggaatta aaaccgaacc agccaaacca aagcagtcct gtaccgatag cagctgccat
    10801 aagactgcta tgctgacttc tttctcctaa aggaatcttt ctttgaccaa gtgtgaggat
    10861 acatgctaaa gagccgaatc cagctgtcgt gtggatgact gctccaccag cgaaatctct
    10921 aaatcccatc tgatccaaaa atccgccacc ccatatccag tggcaaacag ggaaataaat
    10981 cagcagattc caaaaaataa caagtataat atagcctttt aaattcaatc tccctgcaaa
    11041 agctcctgtc atcaaaggaa cggtaatcac gataaacatc agttggtaaa gaaagaacat
    11101 taaaaatgga atcgttgcac catgaaacat gttggggaag cttgtaacat gtctcatcaa
    11161 aaagaaatct gccgggtttc caataacacc gccgatatcc cgtccaaagg ccaatccgaa
    11221 tccaccaaat acccacataa tcgttgttat cccaacggag agaaaagatt gagacatgat
    11281 cgttagtata ttattcttcc gtgctaatcc gccgtaaaaa aaagctagac caggagtcat
    11341 caggcaaacc attgctgtac ataaaatcat gaatgctgta tctcctgtat tgataggtaa
    11401 tgtattcatt tttttcgcct cctttctacg attacgagta taaaagaagg tgtagttgtt
    11461 tttttcttct gtttttttga attaatactt gcatttataa aagacagaca attaataaaa
    11521 gcttgatgtg ttgtgctctt tttcgttttt cacacaaata aagacaaaaa ataaacggtc
    11581 ttctttttat agaaaaccgt ttattttcgt ctttttttat ttttaaagat attttttcat
    11641 aaggaataaa ga
//
LOCUS       DACDRN010000075        11605 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDRN010000075 DACDRN010000000
VERSION     DACDRN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.075"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            269..475
                     /locus_tag="IWJ80_002307"
     CDS             269..475
                     /locus_tag="IWJ80_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP9462003.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(677..1678)
                     /locus_tag="IWJ80_002308"
     CDS             complement(677..1678)
                     /locus_tag="IWJ80_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9462004.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1683..3596)
                     /locus_tag="IWJ80_002309"
     CDS             complement(1683..3596)
                     /locus_tag="IWJ80_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9462005.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3764..4270)
                     /locus_tag="IWJ80_002310"
     CDS             complement(3764..4270)
                     /locus_tag="IWJ80_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9462006.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4430..4870
                     /locus_tag="IWJ80_002311"
     CDS             4430..4870
                     /locus_tag="IWJ80_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9462007.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4896..6053)
                     /locus_tag="IWJ80_002312"
     CDS             complement(4896..6053)
                     /locus_tag="IWJ80_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462008.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6056..6412)
                     /locus_tag="IWJ80_002313"
     CDS             complement(6056..6412)
                     /locus_tag="IWJ80_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462009.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6710..7684)
                     /gene="sgrA"
                     /locus_tag="IWJ80_002314"
     CDS             complement(6710..7684)
                     /gene="sgrA"
                     /locus_tag="IWJ80_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP9462010.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7880..8719)
                     /locus_tag="IWJ80_002315"
     CDS             complement(7880..8719)
                     /locus_tag="IWJ80_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9462011.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8904..9791
                     /locus_tag="IWJ80_002316"
     CDS             8904..9791
                     /locus_tag="IWJ80_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP9462012.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10385..11080)
                     /locus_tag="IWJ80_002317"
     CDS             complement(10385..11080)
                     /locus_tag="IWJ80_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP9462013.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(11064..11462)
                     /locus_tag="IWJ80_002318"
     CDS             complement(11064..11462)
                     /locus_tag="IWJ80_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9462014.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3458 a         2311 c         1739 g         4097 t
ORIGIN      
        1 aagtttagga tacatcgaat ttttggggaa attcttttgg gggtatccta aatcttttct
       61 attatattcc catattttct attattggtc tagacgtatg atggtaaact ttgtacctat
      121 ctgattgctg aatcattaat tgcttatcat tagccatctt tgtttcaagt tcgctagatg
      181 atcattttat tcacatccta acaaacttgt cacatttata gattcttaat ctatttatca
      241 tacaattata aaaaaggagg tgctgcttat gaaagctatc catatttctt tgcttttttt
      301 cgctagtgca ttgattttat ttgctattca tctttgttcc cagccttata tcaataacaa
      361 cggaatatta gtggaaccag cattcttttg cttaccactc ggctttttgt cccttcttgc
      421 ctccgcaggc ttcggatttg ttgctttttt taaacgatca aaacaacaaa tctgacttat
      481 aaaatttacg taaaagagat cgtgacaata atcgtgtgat attcccaaat ttcgaataaa
      541 aggtgcccct tgccatctct aactcctcag tcacaagtta caatattcat aaaccatgaa
      601 aagcttgttt attgaaattg ctccttgtga cttggagcta agtgacagat tacaacctct
      661 tttgcatcat aagactttac tccttgaaaa acattactaa cttttgatat gtttcttgcg
      721 cttgtttatt gctgaaatca aactgatatt catggttcaa aacagcattt ttctccgcat
      781 aatctacttt tgtgactttc acccccgctt tctcaaatac agaagttact tcttccattt
      841 gtttcgtaaa cgtatttgta tttccatccg ttaagaatgt gttttgcgtc caacgatccg
      901 catgtctgac caaggctaaa cttttgacct tctctgattg ttcaaagtcc ttattatcaa
      961 agtaagccca tccacatttt tcataaagaa agctagagat tttatcactt acttgagtaa
     1021 gtgcagaagg atccaacaga ccagacaaag aaataaactt cgtaatctgc ttgttctcca
     1081 aagtcggatc aagatgatca gcttttttaa tttcttcatt ttgaagtgct aacaaatatt
     1141 ctccactaat ttgtgcacct gccgaatttc caccaatcga aatattcgac caatctgctt
     1201 gatcttttat ctctttttta gctgcttgaa tggcttcgtt caattggata actggtgtag
     1261 gatgttttgc ttccggtgat cgttcataat ctatatttac gatgatttgc tgcgtgtctg
     1321 aacatagata ttccaaataa tcacgacaat catttttatc ccctcctaca tatgcgcctc
     1381 catgcatcca gaaaagaatc ggtaattttt tcttttccac tttcggtgtg tagatatcca
     1441 tattattatt tttgtacttt gaaggatagg atatttcttt ttttatatcg atttgttcga
     1501 ttttttgctg gaaatcatct ggtcgggcat atacttgttc tttttcactt gtgtcaaatt
     1561 gttttctaac aagcaagcta atcggctttg gtgtgatttg attaatcaaa aatacagtga
     1621 ctaaaataac tacgatgata actggtactg caatcactat tcgcttgaac catttcattc
     1681 atttaccctc tctttcttcg ccaaatccat gtgataatcg tgctaacaga taaaaatcca
     1741 cctgaataaa tgagaagcga atgtttcttt tctccagttt tcggcaacat tctttttttt
     1801 gcatctgtct ttttttgtac tttcactgtc atctgaatcg gttgcgtatc atcacttgat
     1861 tgatcctctc gaggttcatt ctttccctta ttgtctgcat tctctgtcgg atcttgcttt
     1921 tttggtggag ctactgggat ttttttgtaa tagaaaataa tagttggtat cgtctcatta
     1981 aaaatccctt cagtcggtcc ttcaacattt gctaattcgt atccttcgaa tgtcttttgc
     2041 tctgtcatat aattttctcc ataagctccg taaagctgga tttcgtcctg gtctttgagt
     2101 agacttttat tgttttcatc cacatacttt acgtttatct gatcatggaa cactactttc
     2161 aaaaagtccg ttacatgttt cgtgtaggca gcatggtcaa cttcctgtgc ctttccatgc
     2221 cctgctcctt ctactaataa aagatcttta taccctcctg ataactcata taatttttgc
     2281 gcgttagaca caggtactac atcatctgct gttccatgga taatcaattt aggcaatgaa
     2341 tctgacattc caatcgtagg tagagcagct tttgtatcca aatgcatctc ctgatcaaaa
     2401 tagtattgtt tcaaaaggcc cataaataaa tcttcatgct cttgatcccc aatgatgtct
     2461 aactgatttt ttatggcatc cggtagtgcg ggggctactg tctggctgta cgtaaaacgt
     2521 agttgttcat caattgatgc aaatccgcaa ttttcaataa ttcctttgac attgacagga
     2581 atatctttcg ctaatacaga catcatcgta gctgctccca tcgaaccacc ataaagtaaa
     2641 atcttttgag atggccgctc tcgaacttct tgatttatcc agtaaaggac gtcatctgac
     2701 tcatattgtc cataggtcac ataatcgcct ccgctattac cagtcgctct ggaatcaaca
     2761 aataataaat tatacccggc cttataaaaa tcattgtatt cttctgtaac gatcccgtta
     2821 ttccaattcc ctcgaaatcc gccatggatc atgactgttt tatctgaacc ttgatcgaca
     2881 taatatgcat atacatccat cattttgcca tcttgtttgt ttagtacagt ttgatgaagt
     2941 tgttttgtat ttggatagaa gcctgtgttt tctgtaggcg ttgtcccatc atctgtacct
     3001 ccaagcatcg acatcaacac actgtttttt gcaaaagcga tatcgtacaa tagataagta
     3061 gcaatcagat gtttgataat ctctttatct acaccataat cattaacagc aagctcagtg
     3121 actaataact ctattaattt tgacttgatg tcttctgttg gtccattaag tatttctgtc
     3181 atctgtttac cgaagtaatt caaatattct ttaccattga aattttcacc tttaccgata
     3241 tcatcatttt ctgtcccatt ctctttgtca cttactttag cagaagagga ttcgtcgttt
     3301 gacatttttg tagctgttgc ttcttctgtt tgttggttat ctgtggtagc ggtcgtttct
     3361 tgatctgtgc tagtaacaga tgtctcaacc gttgtttcag tatttgtttc tgtttgtgaa
     3421 gtactcgatt tcgtttcatt tccagcatca gtagtttctg tggtttctac ctcccgagat
     3481 tccgattcgg aagaaatgct ctctccatat actgtccatt gcgggacgct tgtacttaca
     3541 gcaaataaga tgaccaacgc tccctttaat acttgttttg ttctcaagct attcactcct
     3601 ttttatgttt ctaggagaat tatatgtaaa cgcaaacatt ttttatacaa aaaataaatg
     3661 gtttatccca taaaaataga acaggattat tttctcattt tacgtaataa agcagaatgg
     3721 ataaaccagt atccattctg ctttttttaa acgaagaaac aacttattgc tccaaaaatg
     3781 tcagaaatcg tttatcccaa attttctcgt aaaaagcgat tgtttccttt gctttcataa
     3841 aaggattatc ataagttgtc gttgctcctc tacacatcat aagctggtat cctaatccat
     3901 gagcaaactt cacagtcgta tctatacaat actctgcttg tgccccacat aattcgatcg
     3961 aatgacagtc caattcagtt aataaaacac tcaaattagt cttataaaat gaattcgcat
     4021 gggtttttct cacaaaataa ctgtgctcag gaatagataa ttccgacaat agctcccatg
     4081 gttttgtttg tggcactaat tgagcatctt cgtgttgaac gaaaataact ggtttcccaa
     4141 tcgtttcgta tgttttgatt cttttattca ctaatgctgt cactgtttct aattcgtgta
     4201 tcggcgaaga atcaaaacat accccttttt gtaaatcgat cactactatt gcgtcggctg
     4261 tgtttgacat acttgtgcct tctttctttt ttcttgattt aatagtattt cttatctagc
     4321 tagttccatt atacgtgaca acattttctt attttacacc tctaaatggc acttttcgac
     4381 tatttcttca tgttattatt tcataaatac ttagctattg gaggaatgta tgatgattaa
     4441 attgtataca ggttctggct gctcgtcttc aagacgcgca aaacgttggc ttatcgtcca
     4501 tgatataccg tttatcgaac agaacttaat tcgtcaacca ctaaccaaaa atgaatttat
     4561 ccacttacta aaattgacaa acaacggcgt gaccgaaatg ctctctactc attctattag
     4621 ttataaaaat cttgttcaca gaatagatga actcagtttg agtgaactat atattgagat
     4681 tcgtacatcc ccttctttat tgaaaaaacc gattatctac gaccaatctg caaaactttt
     4741 gactggattt agcgaagata aaatcaggat gttcctttct aaaactaaaa gaaagcaaga
     4801 gctcaacgaa ctcttgcttt ctggtcatct tgtcgatttt aaagaaaata atctttttgc
     4861 aatgtcttaa ttttggcata aacaatctgc ttttcttaat taacgaaatc caatttttcc
     4921 aatatggatc tatttttctc attcctgatt tttatgattt cagctttcag acgcgcccaa
     4981 tcattttttg taggttgtcc attccagtaa aatatctgtg tattcgtttg atcatatgcc
     5041 tttttgtcga taggataatc gctaatgaca atatcaggta actgatcagc ggatgcacta
     5101 tattttattg ggcgttcagt catagataat atccttttct ttgcccataa ttcctgtgtt
     5161 ttaccatact ttgattgaat atagaatatg acaggttgtt cgtgttttat aaaaatatcc
     5221 ctgactaaca tacagtattg aaaaatcaac cgtctgttct tttcataata agtattaagc
     5281 tctggaattc tgcgagctac cccactaaaa aactgttgca tgattggata catcactgga
     5341 aatatatgct gaaaatctct gtcttctgct gattttccaa aagcatccac cttctttttt
     5401 gtcccaaaaa cccttgaata caaatggatc attgccaaat tgacatatag ataatttttt
     5461 tctgaagttg taaaacaaat cttgaagttt ttttcaaaat tttctacaat agacgattca
     5521 atccagcccc aactttcttg tactttatct ggagtatcca acaaatcttc ttccaaatag
     5581 gtgttcatta caccaaacat aaaataaagg taagcttgct ctgttggagt gatgtgattc
     5641 atcgaccaga agtttgcttc aaaaaaaggc tctagtatgt aatgcgtaaa ctcttctttt
     5701 gttatcaatg gatgcacaaa tttgttcatc tcttctggga catacgtaat cgagtggcct
     5761 tgtttcattc gttgcaacga aattcttagt aggctgatcc atttctttct atctatacta
     5821 cgcgtatatg gcgcatgttt cgcaaatttt tctaatagtt gacgtttatt ttttatactt
     5881 gataatatat cagaatgatt ttcgtcgaat gactgccaat acatcaagtg aaaaaagtat
     5941 cggatacggt attcttctcc ttctatcgga ttaccttttt tcaagctgca agataaatga
     6001 tagtttttca aaatagtatt aagttctctt aattttcttt gaatcgtacg caaacttata
     6061 taattttttg actaagattt tctaaggatt gataagtacc tttgtaaagt gaatccagta
     6121 aaatataacc aatcgttccc tctaaaagta tagaataaac tttactcagt aaaaactgag
     6181 aagaaaactc gacttttatc atcttgtttt ctgaaagaaa agataattta tattctggat
     6241 acctttcgca aaacgattct gcttgttgaa tcaccgaatt catcttagcc agggtgtagt
     6301 ttagatctaa agcgatatcc ttcaccacat ataatttctc gtctctttca taacaatagt
     6361 tcaaaaattg atatacttgc ctttcattga tatcaataaa ttcttcaaac aaagtacata
     6421 ctccttttta tctattattt agacttattc cgacagctac atgaataacg tacaaataga
     6481 ttcgttactg atttttcaga gatactacca tgcgcagtgt gcattgtcaa ttattcttgc
     6541 tcttattttc catttgtgtt cctgttattt cctgtttctc aaatatgtta gtaaacaaaa
     6601 atagatctgg caaagctaca acttcgccag gcaatacagc actctcgaat gaaatattca
     6661 ctcaaaaatc tatcgtttta ttcaatttta gatctgttgc ttttattctt tatttcactc
     6721 gatggaagag agaacgaaaa tagcttgctc ctacaatcaa aatgagtaac acacccatcc
     6781 atgtaattag cttgttcaag gttctactac cagtttctgg taaatcgctt tttctattat
     6841 ctgtttcttt cgctttttct tgatgatcaa ttccacgaga aacaaaatca ggctgtttta
     6901 gcacactttt taggttgctc cctgcatttc tcttttgttc cttagttggt atgataggta
     6961 ccaaatcaag ttcattattt acttttcctg tgctgctttc gctgctcgtg cttcctgtgc
     7021 tactctcact actcatgctt cctgtgctac tttcactact cgtactctcc gtactgcttt
     7081 cgctgctcgt gcttcctgtg ctactttcac tactcgtact ctccgtactg ctttcgctgc
     7141 tcgtgttttc tgtgctactt tcactgctcg tactctctgt actgctttca ccgctcgtgt
     7201 tttctgtact gctttcacta cttgtgctat ccgtactgct tggcggtcca acaactgtcc
     7261 aactgcttgg tttcaaattc tccgtgatcg tcatctgtcc atcataaaaa acaatcaaat
     7321 caactttatc tttactcttg atctcatcaa tcgatagttc tgcagaaatc attattgtat
     7381 actcaatacc agcggtccat tgtacgtctt ttaaataaac ttccccaata actttttctg
     7441 aacttccatt accatcccaa ccatatgtta gaacagcatg tacatcacta gcattcacgc
     7501 ttttgtttgg tttgactgga atagtaatga cttgttgtgt catgtcataa acaatccgat
     7561 cagttttcac agtaaatgaa agattctcat ttgcccgttc atttgcagaa gtactgattc
     7621 caatcattgc tgcaattata ctcagcccaa gaataacaaa taatacggtc gctgttttct
     7681 tcatttctat cactctccat ataattacct tctatttgat aaaggaatta tataaaatga
     7741 aaatgatgag aacacgacag tcagttgtga aaatctaaca aaagatgtcg tgtattcgct
     7801 tgaatttgtt tactttttcg tttaatcaat gacagactta aaaaagcaag accgatatca
     7861 gccttgcttt cttcatcttt tattgataaa cagcagtgta tttcttcatc cgctttgttt
     7921 tatatgcata gagtaacaaa ccaacaatca agactaacaa gaataatgta gttgatatag
     7981 atgcttcagc cccaaagttt cccccagtaa ttattgtatt ttgcgattgg atcagagtgg
     8041 agaaaaaacc ttgttcttgc gttccgctga ctggtatacc caaaatagct ccttgtgtcc
     8101 aattccaagt aatatgagca ccaatagccg taaataaact tcctgtactg tagataagtg
     8161 cgctgaaaaa gcaacctgct aaaaagatat tgaaaactgc cagaaatgtc atgttaggat
     8221 tcaatccgtg caaaagagca aataatgcag cagatccaat cattgctatt aacggaccaa
     8281 atctaagcat ctgatgctga aaaaagccac ggaataaaag ctcttctgat gcccccactg
     8341 caataaataa aataaaactc gttatcagcg gcacaatgtt tgcgtttcct gaatctaacc
     8401 gatacgcccc cgtcaaatat aatggactag taataagcag aaacactcca attccaacaa
     8461 cgagacctcc cgctaaatac ccccagtctt ttcctcgaac agaaacacca taatcttcta
     8521 ttctttttcc ttcgacctta ctagcaagaa ataaagctag taaaaaaaca gctaaatgtc
     8581 cgaccgtacc ggcaattgct ccaatccatt cactatttcc aataaacata tccgcaagtg
     8641 cattattgac aagaattcct aaaacaaaac caactaagac tattaaaaca gatccaatca
     8701 accgaataat cctgatcatc tttcatccct cctttatatt aagtttaaca acccttttct
     8761 ttcttgtctc gtgaaaacta tgtaaatgtt ctccatcata gagagaatag atttttacga
     8821 actaagaata tctacagcaa ccttacgaaa atgaaagact gtctccctaa ttcttctgtt
     8881 agactgtttt taataaggaa aggatgggaa attttttgaa aaaacaacta ctaattatgt
     8941 gcctttcagc atgtgcaatt ttactattct caggttgtag caagtccaat gaaacgattg
     9001 caacgtttaa aggtggaaca cttacagttc aggattttta tgataaccca aaagttaaat
     9061 caaataatca aacactatta aaaaaaatga ttgtcttcaa agcatttgat gatgtatacg
     9121 gaaaagagat ttcttcaaag gaagtaacaa aagaatataa tgaacaaatc aaaaaactcg
     9181 gtccaaacta caaggaacaa ttaaaagcag ttggccaaac agaagagaca tataagaaac
     9241 tctttaaaca gatgctggct ttccaatacg gtttgaaagc caatgtaaaa ttaacagata
     9301 aagatctaga cactgcttgg aaagaattct atccagaagt tagtacacag atcattctat
     9361 tttcgactaa agaagaagca gataaagcaa aaaaagaagc aaatgaagga gaaaatttct
     9421 ccaagcttgt tcaagcttat ggaaaaaata aaacattaaa ggaaactgat ggaaaaatga
     9481 attttgattc aaccaatcca gagattccta ctgaagtaaa aaaagcagca tttaaactaa
     9541 aaaacgggga agtttctgat atcattcctg taacagatcc aacgacttat cagcaaagct
     9601 attatctagt aaaaatggta aaaaaacagg ataaaggttc taataaagac aaatacaaat
     9661 ctgagctaga aaaaattgct accgaagcaa aaactcttga tacggaattc atggataaaa
     9721 caatccgtaa agtgatgaaa aaagacaatg taactattaa agatccttat gtaaaaaata
     9781 tattcaaata aacaaatttg acccaattgg ggatgtggca aaatttgaga agtgattttt
     9841 ggaaattcct tcttattttt gaggcaaacc acttttgcca caacttcatc ggggcctttt
     9901 tttatttgta ccaaaacaaa tcactggatt tgttacaatc atgaggatat aatttaaaaa
     9961 agttttttat ttctatcaca tgaatcaatg cgccattaag tagattcttt catgcttccc
    10021 tattttttca cttgccgtat cgcttcaaaa acaactatca taaactcaaa ctcgactgaa
    10081 aagttatttt aaaaaagact aaaaatatcc cttctggact tgttcttctt attctgttat
    10141 actagagaaa cacgaaagat atttttatgc gacacttttc atttgttttt atgaaattta
    10201 ttcaaaactg gcgtaacttc agagttacaa ggatgtatta gaggttaggg aatacatcgt
    10261 ttaaatgaga aataagtaat agttagtaat agtcacccaa actttaaatt aatgaatatt
    10321 gaatcgtcag atagcctcct cgagttttcg agaaggctat cgtttatttt atggagaaat
    10381 tgattcagaa gtaatcagcg acctttttta gttcaccaat cgtttctgct tggaaagttt
    10441 gcacccaatc ttcttggaag cccctcactg ctttatcaat cgagggcatc tctaatactt
    10501 gatctagtag ctctttacct aacgttatcg catgagctcc agatcgaatg gctgtagtga
    10561 cttgttcaca atttttgaaa ctagctgcta atatttttgt tggtctttgc gtacgttcga
    10621 tctcagtccg caacgaatca atcactttca tagcatcaat gccttgattt tgcatcctgt
    10681 tataataagg tgcaatataa tctgctccat tattaatagc taaataccct tgtaattctg
    10741 tgtaaatagc tgtaccggta atcaaataac cttctttttt caactttttg atcgcttgaa
    10801 ggcctgcttg attcaccggc accttgataa acgtatcttt tcccagttct tctatgataa
    10861 ctcgagcatc ctctatcatt tcttcttccg ttcttcctat tgtctgaata tggataggaa
    10921 tctgtccgat tatttttttg atttctttca tatgcgagaa gaagtttgac gctccttctt
    10981 ttttcagaat tgtaggattt gttgttacgc ctgttaaatt caaaaactgt tgacaagaat
    11041 aaatactttt tgtatttgct gtatcaagca tgatttccat atagctttcc cccattctga
    11101 tcctcttgat cgattttaat gattgtcccc tttcttaaat gcggcatttc cttatcttct
    11161 acatacattg ttcctggaag ttccggctca tgagatccat caaattttat cgtgatatgt
    11221 cctaattcat tcaaattagt ttggacaaca tctcctactg ctgtgattac atacatttgt
    11281 ttatcaaaac agagcttcat atcacaagtt atttcttttc ttacctgatt caattcaatc
    11341 ttataacaat aatctgttaa tgtttccggt gcttcatctc caaataaaat cattattttt
    11401 tctttcctga atacttctgc ttctatacca acatctgtta caaccgtttc aaagatcgtc
    11461 atctctgttc ctcctcgtaa aatatcattt gtctaatagt ttttattcaa aatgtttcta
    11521 gattataaaa tttattgaat aacacattcc taacggaatt tgtttcgata ttgtgtgatt
    11581 tctcagagta caatggatgt atgga
//
LOCUS       DACDRN010000076        11605 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDRN010000076 DACDRN010000000
VERSION     DACDRN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.076"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            183..703
                     /locus_tag="IWJ80_002319"
                     /pseudo
     CDS             183..703
                     /locus_tag="IWJ80_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(605..1321)
                     /locus_tag="IWJ80_002320"
     CDS             complement(605..1321)
                     /locus_tag="IWJ80_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP9462015.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(1445..1630)
                     /locus_tag="IWJ80_002321"
     CDS             complement(1445..1630)
                     /locus_tag="IWJ80_002321"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462016.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            1857..3130
                     /locus_tag="IWJ80_002322"
                     /pseudo
     CDS             1857..3130
                     /locus_tag="IWJ80_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3237..4268)
                     /locus_tag="IWJ80_002323"
     CDS             complement(3237..4268)
                     /locus_tag="IWJ80_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9462017.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(4275..5105)
                     /locus_tag="IWJ80_002324"
     CDS             complement(4275..5105)
                     /locus_tag="IWJ80_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9462018.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(5102..5908)
                     /locus_tag="IWJ80_002325"
     CDS             complement(5102..5908)
                     /locus_tag="IWJ80_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9462019.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5914..6738)
                     /locus_tag="IWJ80_002326"
     CDS             complement(5914..6738)
                     /locus_tag="IWJ80_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP9462020.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6728..7828)
                     /locus_tag="IWJ80_002327"
     CDS             complement(6728..7828)
                     /locus_tag="IWJ80_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9462021.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            8006..8791
                     /locus_tag="IWJ80_002328"
     CDS             8006..8791
                     /locus_tag="IWJ80_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP9462022.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8824..9207
                     /locus_tag="IWJ80_002329"
     CDS             8824..9207
                     /locus_tag="IWJ80_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9462023.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            9283..9414
                     /locus_tag="IWJ80_002330"
     CDS             9283..9414
                     /locus_tag="IWJ80_002330"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9462024.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9383..9619
                     /locus_tag="IWJ80_002331"
     CDS             9383..9619
                     /locus_tag="IWJ80_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9462025.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9697..10668)
                     /locus_tag="IWJ80_002332"
     CDS             complement(9697..10668)
                     /locus_tag="IWJ80_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP9462026.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            11302..>11605
                     /locus_tag="IWJ80_002333"
     CDS             11302..>11605
                     /locus_tag="IWJ80_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9462027.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGY"
BASE COUNT         3466 a         2660 c         2210 g         3269 t
ORIGIN      
        1 ttaataaatg caaacagaca ccttgaaatc ctttagaaaa aaggcttcta taatattttg
       61 tgttgttgga aaatcgaagg cgattttcta ctaacacttt ttcttcgttt caactcaaaa
      121 agagactcac ttttggtaaa atttagtcga ccaaaacatc accctaccaa aggagtcgct
      181 ctatggataa tcatactaga aaattactcg gattaacaga taaacattta ttttttgatg
      241 aagaatggct gattgaaaaa gaatataaag gcgttcaagc gcagtttatt aaagggagat
      301 tggatgactc accttcccat tgtgaacact gtggatcgat tcgtattatc cgcaacggtt
      361 cctatacgac acgtactcag atgttgaaag tcaaagaaaa gttgactatt ttagaattaa
      421 aacgtacccg ttttctatgc tatgattgtg ggcagacctt ttctgcgaaa acggacttag
      481 ttgatgaaca tcatcaacta acaaaggaac tgaagcaggc gattctcata gaactgtacg
      541 aaaatcaatc acgtaatctg aaaatcagaa gaagtaccgt cggctcaagc gatattggaa
      601 actcttactc aaagattcca cgacattgga gccgctcaaa cggcattatc atcgactgtt
      661 taaacgacca attagtcaaa cagaaatcgt cgatgaaccg ttgtgcacaa tggtgtcctg
      721 aacgtacagc gacaccttcc atatccagag cagtcgccac atcacgggga tggatgccat
      781 ccaaattaaa agcaaccacg ccagtatgat gttcaggatc ttgcggtccg taaatggtga
      841 taccgccgat cgcttgtaat ttaggtaaaa cgtaggcaac cagttcttgc tcataacgat
      901 gaatattctc cataccaatc tcattcaagt aatcgattgc tcctgcaatg ttcggagtac
      961 cagcttcaaa tttccatggc agctctttcc atgaactatc gtaaagattg acaaaatcta
     1021 tcatttcacc gccgaattcg actggctcca tttgttctaa caattgacgt ttgccatata
     1081 atacaccgat tcctgtcggc ccgcacattt tatgcccgct aaaggcgaaa aaatctgcat
     1141 ccaacgcttg tacatcaact gtcatatgcg gtgctgcttg ggcaccgact ggactatcct
     1201 caactacaac agcctgttca accctcagcc gtttcaagca caacaaataa ccttctggat
     1261 ctggtttgcc gacctgaaga tcatcgatcg tcacaaagga atcaattttt tccttaatca
     1321 tcggatgtgc ttcaaaaaaa tagtctagct cccgcagttc agaattgaga ctactccgat
     1381 tttccagtgc tgctgatccg ctgctcaaaa tgttcagcat ccactggtct cccaaccgct
     1441 tgacttatcc cttcataaaa ggacagttct gctgccgtcc caacaccttg ttgatagtct
     1501 gcaaaaggaa ttttatagcc gaactgctcc agtaaatagg cttcagtaaa tagtgatgaa
     1561 tactctcgta aaacatccat tcactgtcaa tcatcgtccc atcaaaatca aaaataagtg
     1621 ctttggtcat cttctttccc ccttacccaa aacaagcaaa agctgtgtcc tgctctctct
     1681 acagtatcag cataaaaact gccgtctgaa aagtcaaagg tgaaagaaat gtggcgctat
     1741 aatttctagg actgatagta aatcattcta tcagtccttt tgagcataaa aaaacaaaac
     1801 atacagaagc tccgttagaa ttgaatcacc acaacacaat caaaggagcg ttctgtatgc
     1861 ctgatcttca tcttatcaaa ctattactag gaatcaaaga tggaaacatt caaatcactc
     1921 atgtcgagaa gaaaaaaata cattcaatca acagtatcgt tgtttctgcc acgctcatta
     1981 aaacgattca gccctgtcct cagtgtaaac aggtgaatca acatgggctg atcgttaaga
     2041 acggaaagaa aaaatcgctg gtccttctca ataagtgtgc gaatcaacgg acttatcttg
     2101 ctttggcaaa gcaacggtac ctctgtcgtg gctgtcacac ctattttacg gcgaatacct
     2161 acattgttga aaaaaactgt tttatcgcca aacaagtaca ttataaaata ttggaggaat
     2221 tgactgaaaa acaagcaatg acgacgattt ctaaacattg cggcgtttct tggtcaaccg
     2281 tttcgcgaac actcgcttct ctgctcccca tgacaaacgt gaaaataaat tggttgcctc
     2341 gctgttcatt ggtagatgaa tttcgttcat tgaaaaatca agtcggtccc tattcttttt
     2401 cttgtatgga tggcgatact gggaaattac tggatattct tccttcacgt aagaagaaag
     2461 atctcatttc ttattttatg caatttgaac gacgtgcacg tttaaacgta aaaatactcg
     2521 taacagatat gaacgcctct tatgctttgc tgataaaaga gtgctttcct catgccaaac
     2581 tcgttgtgga tcgctttcac atcgtcaagc acctcattcg cagttttaag gatatttgac
     2641 tatgagtcat gaaaagcttc gaccgaaacg atccaataca agccaaacac tatcgacaag
     2701 tgaaggcttt atctcgactt ctgatcaaac gacaggacac gcttgtttat gataaatgga
     2761 caaaatggcg taattttggt tgggcctatt taaccgaaag tgaagtcgta gaacgtctac
     2821 tttctacttc tgatgaacta cgaatagctt atgcttacta tcaagaaatt cttcaagctt
     2881 tttatgacaa agaagcggac atcttctttc agcttgtaaa aacaatgccc aagtcggttc
     2941 caagagaact gcatcatata aaaaaagcgt ttataaacta tgaatccggc attcgtttag
     3001 ccttagagtt gccttactca aatggcaaaa tagaaaatct gcatacccac atcaaagcat
     3061 tgaaacgaat agcttacggg ttaggatgag aaccagaatt ttcttaatca atcatctgat
     3121 cacttattaa acataaaaaa acatctaaag cagaaaatca attctacctt agatgttctt
     3181 atatcactct atcagtccta tttgacaaag agccagaaat gtttgccgac tatacactag
     3241 ctttgttgtg caaaatgctg agtatcaatt tccacaggga ttgctagaat atctttcagc
     3301 tgaatgtata aggcatcgct aattttctct tcccgatcgt tcaaccgatc cactaaaatt
     3361 tctttgccct ccagcgtttg aaaaacatac cgttgaatgt tgcctaaaat aaatgattcc
     3421 ttccatagaa ccggaatctt aaccccctga ggaaccggtt caaattgaat cagctccggt
     3481 cttaaataat aaatgcagcg attgccctta gcggaaaact cgtgggtata ctgattcagc
     3541 tcataccatt cgaaacgatt atagttgccg ataaattccg caacaaatcg gctctgagga
     3601 ttctgataca attccttcga ggtggaaacc tgagccaccc gcccagcttc catcacaaaa
     3661 atccgatcac tgattcgcat cgcctcttct tggtcatgag tcacaaaaat catcgtcatc
     3721 cctagcgatt gctggatagc gcgcaaatcc cgctgcagct gttttctaat tcgggcatcc
     3781 aaagcactta atggctcatc cagcagcagg actttcggtt cagtgaccaa tgagcgagcc
     3841 agtgccaccc gttgtcgttg accgccagaa agaaactgga catttttatc tgcctgctcc
     3901 gtcatatcca ccaatgatag catctcaaaa accttttcct gaatcacctc aggactcagc
     3961 ttctttacct tcaaaccaaa ggctacattt tcaaaaacag tcatagttgg aaacaatgca
     4021 tactgttgaa aaacaaagcc gatattgcga tccttcgtag gcttatcggc aatattctca
     4081 ttgtccagca caatctcacc gctctttggt tgttccaatc ccgtgataca gcgcagcaac
     4141 gtagactttc cgcacccgct gggacctagt aaagtaacca gctcaccctt ttcaatctga
     4201 atttcatcaa tagctaaaat ctgtttttca tcaaaaaaca gattgagatc atgtatttct
     4261 aaaaacattt tccgtcacaa ctcctttaag ggcttctttt tcgactgctt ctcttccctc
     4321 gtctgcttaa gggacagctg ttgcttttgt cgataagtca gcataaaggt agttaaagca
     4381 attcccacca taaacagcaa atacaaaacc atcacagcgc tagccaaatg gccgttttcg
     4441 ttcatccgcc gcagcatata gatgcgtacc gtctgaaatc gaccgccaat caacaggttc
     4501 gtcagcatgt actccccaaa aaccgaagaa aaaatcatca gagaaactag ggtcacaccg
     4561 atacggatgt ttggaaacag cacctggata ataatcgtag acatcggggc gcccaaggtt
     4621 tctgctgcat ccactaattc cttcagatta attaagcgca tctggttgtt aagactaaga
     4681 tagacaatcg gcaacgccgt aataaacaat gcacctacca acactacaaa cattgggata
     4741 ccagtttcag catacgttcg aagcaaagcc gttaccaaaa tcactcctgg taaagcatac
     4801 ggcagcagga gcagcttttc cagccaacga ttcaaacgag ggaaatacaa atgaatccaa
     4861 ataatggtgg gaatcatcaa caacagaatc gtcaccaaga ccacacctgc taataaaagt
     4921 gagcgaccca ccgcagcaat aaattccctg tccgtcagca gctgttggaa ccattgcaag
     4981 gtaaaatcac taggtaaaag cgattttgac catttagtgg agctggcata caaaatcgtt
     5041 ccaataatcg gcagcaagta atacaggaca aagcagaaca aaatcggttt tattaacttt
     5101 ttcacgtctg caccaccttg cctttctttt gaataatcaa cattgccgtt accagcagga
     5161 cacctaaagc gactgccaac gcactgccta aattaggctt ggcgaacaca tctcccgaca
     5221 ctaaagccgc aattcgaatc gttaaaagat ttacattact gctggtcagg gcatacgctg
     5281 tttcataggt ccccatgcca ttagcaaata agatgacaaa ggtgctggca atcgatggtc
     5341 gcaaaaaagg caaagccacc ttgaaccaag catacgctga tgaagcgccc agcaattgtg
     5401 cggactccag ccattccttt ttcacttcct tcatgctggg atacaaaaac aagaccccta
     5461 acggaatttg gaaataggta tacgtgatgg tcaatcccca ccaagaatac aaattgaagt
     5521 ccgctagaaa tggcaaaatc attttcagca cacctacatt ccccaacaaa ataataaaag
     5581 agaatgccag cggaatccca gcaaaattag ctgccaaatt ggaaacaaag gtgattttct
     5641 cctgcaattt agtcgataga ttcattaagc ataaacataa aataaacgtc ccgattaacc
     5701 caatcacact ggagctgaca gcaatcagta cgctgttaac aaaggcctgt gtgtaatatt
     5761 tgttgctgaa aatatcactg taattggcca atgtccactg gttagttaaa tcactgtgca
     5821 agctgcacag aaccataaaa agcagcggca gcagtaaaaa tgcacagaag ataaaggagg
     5881 caggaaccag aaccaagcgt tttttcatct ctttcatccc ctcatcaatt cttgaatgtc
     5941 ctgcattttc tctgacggtg caattcctaa taaccagcaa aacaatggag ccgccagcag
     6001 ctgtggtaca gtttgttcct tcagcttttg gcgtttattt gaaatcaaat aaaaaggaac
     6061 ttcacgatgt gcagccaagc tgccgccatg cagtccgtgc gcatccatcc cgtgatccgc
     6121 agtcaccacg acttgataac ctttttgcag ccattcagga aggtaaatag ccaataatct
     6181 gtccgcctga ttaaccgctg ctgcatattc atgagaatcg gcggtaaatt tgtgaccgac
     6241 atcatcaata ttcatcgaat gtacgaccaa aaagtctggc tgatactgat tcactaaata
     6301 atgggcatcc gcaaataaat gcgaatcagg gtaagtgtcc tcactataaa agatgccatg
     6361 ctgaatcagt cgctcctcct gatgctggat gcgatcaacc aaacgattat agggggcttg
     6421 attgtacaac tcgctgtacc agtggtaagc ggcggctcct gtttttccgc cagcttcctt
     6481 aactaattca aacagcgacc actctttgga tcgctgagca tggtaattac tatagatgcc
     6541 attttcatag gctggaacgc cggtcatcag tacttcgtat aacggacgtg aattgctggg
     6601 cagctcactt tttaccttca ataagcttgc ttgctggtgc tcaactaaat gattcatgaa
     6661 cccaagctgg gcgacggctg tgtcgtagcg acagccgtct agtataacta caatcagttt
     6721 gtcaagtcta ctttgcatag gtcagtacct ccgcctgcca aagctctgga agctctacag
     6781 aggtcttgtt ccaagcttca ttgtcttcaa ctggcaccgc cgcttcataa gcatcctccg
     6841 gcagtaattt ttcagctact tctttaggca gctctacctt gcgaataggt cgcgcatagc
     6901 cctttgccaa attgatttgc ccttcatcag ataaaatata ttcccgtgct agctttgccg
     6961 cattgggatg aggggcattt ttattgatta aggttgtata cccgctaatg atggagccgt
     7021 ctgttggcac agttacctca aaacgctcgg agtcaatttg gtcacgataa tttagtccat
     7081 tgaagtccca aacaactgcc acatcgattt cacctttttc aagatttgct acgctggcat
     7141 caacggtgga caaacggcct ttttctgcca tttttttgta gtaattcaga cctggctgaa
     7201 tatccttttc gtcacctccg ttggcaatcg ccgcagccaa tacagcaaat tgtgcttggt
     7261 tggcaatcgt cacatccccc acgctaactt gatagtcacc ctcttgcagc tccttccagc
     7321 tagtcggtgc ttgcttcaca ttctctttat cggtgataaa ggaaatggtt cccgtatagc
     7381 ttaacaacca atccccatca tcatcctttg cccaatccgg aatttcctcc caatagttgg
     7441 tcttataagg taaggttaac tctttgcttt cagccaacgg accgaagctg ataccgacat
     7501 caccaatatc ggcggttcca ttttttcctt ctgattcaaa cttagccaat tcttctgcac
     7561 tggacatatc cgtatccacg tgacttaagc cgtattcttt ttcaaggtcg ttccaagtgc
     7621 ctacccaatt tgcccaagta tccggcattc ccaagctggc aatctctcct tcttccttgg
     7681 ctttttctat aatcgtatct aaagagtcgt ctgccgcagc cgattgcttc gttgcttcac
     7741 ctgagccgca gcctgtcagc aggatactta ctgttgctgc acaaaataaa ccgatagtca
     7801 tgttacgaat tttttcattc attttcattc attcttctcc cctttcgata attccattct
     7861 aacaaagctt tgtaatgaga gaatcagttt cttgaaaagg tttcatgaac aatgtaaagg
     7921 ttgtatatgg tttgtaaatt tcgacgaatt cccgttaacc ttctaaagct tttgcggtat
     7981 gatagtagaa aaaaggaggc tccctatgca gtattatgat cagcatctcc acacgtattt
     8041 ttcctttgat tcagaggaaa aatttgaaaa ctatttagcc tatcagccgg agtttttcgt
     8101 ttctacggat cactttgatt taaacaatcc atctaccgga aaagatgaca ttccggatta
     8161 tccagcctat ataaacaaac tggactcttt aaaagaaacc tattccacca aatttctgcg
     8221 aggtatcgag attggcgttg ttccaggaca agaagagcgg atcgtggatt acctcgctca
     8281 gcatccttat gatttaaagc tggtaagtat tcatcaaaat ggccgctttg attatatgga
     8341 cgatgtggtt ttaaccaaag atcccattca aactaccaag gaatattttg atcagatgtt
     8401 gcaggtcttg acggattttc cagaaggaga tattttgact cactttgatt atggattacg
     8461 ccgcttcacc ttcacagcgg aagaattcgc ggagcatttt gaggctcagc tgctaacgat
     8521 tttcaaaaag gttgttgaac gagaattggc gatggagtta aatgccaaga gcttcttgaa
     8581 atacggcaac gaagaccttt accgttatgc cgttcctttg tatcaaagtg tcggcggaaa
     8641 gctctttacc ttaggctcgg atgctcatgt tgccgaagat tatcagctgg gctttaaaaa
     8701 aatgagtcag ctgttggcag actgtcaggt agaaaagttg ctagtcattc aaggaaaaga
     8761 acgccgcctg acgccgctgc cgattttgtg attcctatat aatagaagaa agtggtgaaa
     8821 aaaatgagca acctgtggga ctacaatcaa gaggcaccca tccattattt aattgcgcga
     8881 cattgggatg cgttgaaaat tgaggcggtt tgccgatcct tgttagcagc cgttccaaaa
     8941 caacaactgg aaaatttttt ggtggctgac agcttgcagc gggaaaaagt tcaggcttat
     9001 tttgcagcct ttaaagatca gcctttggaa tatttgcatg cgcagtttca tcttttttat
     9061 caagtagccg ccccagatga ctacaacgat ttacgaggac agctgcaatt gacctttcaa
     9121 gcggatgaaa ccgcctatac cgttttattg ggaatggctc gccttggtga tcaggcgaag
     9181 gtcgagtggc ggattttcga tatttgatgg caggatcaag gcgtttcagt tgcaaacctt
     9241 cctctctttc aattagaaaa accgcagcag gaagaagttt gtgtgctggt caccgatgct
     9301 ggtgaaattc gtctccgctt atttcctgac caagctcccc tggctgtagc caactggcgc
     9361 tccctagctg agcaaggcgt ttatgacaac acacctttcg gctcgtgtca ttaaggaatt
     9421 tgttattcaa ggtggcgctt tggatggttc tggtgatgaa gctgtttcca gctatgaggg
     9481 ctttttcgct gatgaagtgc atcgcggact ttatcatttt aatggcgcct tggccttagg
     9541 ggaccacggc ccccacacca acggcaacca gttttttatt gtgcaaaaca caaaggcgca
     9601 ggctgacctg ctaatgtaaa gcaagcatac ttggagcttg gaggactacc ggagctggat
     9661 ggtcgttata ctgtttttgg acaggtatac caagggttag aaatcgtagc agcgatcgca
     9721 gctcagccga caaacagtga agatgtaccg attgagccag tgttcattcg acaaattaac
     9781 ttcgaaaaaa catcgtaaaa agacactcac tgacttcagc aaaagctcta ttcttgagct
     9841 ggctgcagct agtgagtgtc tttcatttat ccataagata aagcttggat tcaataaata
     9901 aataaatgtt ttggttaaag ctgtgaccag caaatgagga accgtgcgag ccagttgatc
     9961 tgccaatgca aacatttctt taggtgaagg cgctggcgtg tctgccaacg gatactccag
    10021 tcccaatgct tggtaaccgt gcataccata gcagtggtac ggtagtaatt ctaacggtac
    10081 gaaccgatca agatctgcca caaaacaagc aatcgtcagc agctcggtca ggttcatatt
    10141 gatacctgga accacaggaa ctcgaattcg cacttcgcag ttaggatact caattaaaaa
    10201 gcgacgaata ttcttcaaaa tttgttggtt atctaagccg gtccattgtt tgtggagctt
    10261 gctggtaaac atttttacat ccacaaacaa tgtatccaca tagggagcta ccttttggat
    10321 ttcattataa gcaccacaaa agctggtttc cagtacggta ttgattccaa tgtatttcga
    10381 tttttgcaaa atttctttag caaaatccgc ctgaaccagt gcctcgcctc cgctaatggt
    10441 cactccgcca tccgagtgaa agtaaaaaac agcatccttc tcaatctcat ccattacctc
    10501 ttcagcggtc atggtctggc caaatccttt ttcaatctgc ttgctttgtg attcgggaat
    10561 cgaacaccag gcacagcgta atgggcagcc ctttaaataa acaacggtcc gtaaaccagc
    10621 accatcatgt agagaaccct gttctattct taatactgtt ccttccatat agatttttga
    10681 attacacact agtttaaact aaaacacaca aggaaaaact taccatttaa tcataaaatt
    10741 agctcatata aatatactag gatgtaaaat agactggtca aaatctggtc aaaaaagagg
    10801 actaatacag gtagtcctct tttttgttat tttaatattc taaatcattt tttattggtt
    10861 tattccctta ttcgatgtag actcaaatca ctatatcact agatatcaaa cggcgtcata
    10921 cggaatcaaa aacatcaaat ttgacctgtt aaatggtcat tgaataacat tcaaaatcaa
    10981 taaaacggag tcagacacgg agtcaaaaac gctgagagta aaaaatatat acagccttgt
    11041 aggtaaagta ccacagggct gtatattttt tatttcagat taccttaaat gattccattt
    11101 actttttatc tcattttttc ctactagtta gtatcaaata ctctcctatt ttctcaataa
    11161 actatttttg gatacatggt atctgaaaca gctaaaacct tgtcattaca gcaaaaaatc
    11221 acgttatcca gactgtagac aaagtccatt ttatggatga tgtctacagt cttttctttt
    11281 ataatagatt tatgaaaacg aatgcaagga gtgatagcta tgatgtctaa aaacaatacg
    11341 aatggtagaa atcaatttgc gatgcttacg attgatgatc tagttcctca agatcatctc
    11401 gttcgaaaga ttgatgccgc gcttgatttt gaatttattt atccaatcgt cgaagccact
    11461 tactccgatt taggtcgccc aagtatcgat ccagtgattc tgatcaaatt ggtgtttatt
    11521 cagtatttat tcggtatccg ttccatgcga caaaccatta aggaagtcga taccaatgtc
    11581 gcttaccgct ggtttttagg ctact
//
LOCUS       DACDRN010000077        11510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDRN010000077 DACDRN010000000
VERSION     DACDRN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.077"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1029)
                     /locus_tag="IWJ80_002334"
     CDS             complement(<1..1029)
                     /locus_tag="IWJ80_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9462028.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLRDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1111..1800)
                     /locus_tag="IWJ80_002335"
     CDS             complement(1111..1800)
                     /locus_tag="IWJ80_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9462029.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1906..2274)
                     /gene="rplL"
                     /locus_tag="IWJ80_002336"
     CDS             complement(1906..2274)
                     /gene="rplL"
                     /locus_tag="IWJ80_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP9462030.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2327..2830)
                     /locus_tag="IWJ80_002337"
     CDS             complement(2327..2830)
                     /locus_tag="IWJ80_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP9462031.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3240..3929)
                     /gene="rplA"
                     /locus_tag="IWJ80_002338"
     CDS             complement(3240..3929)
                     /gene="rplA"
                     /locus_tag="IWJ80_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP9462032.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4033..4455)
                     /gene="rplK"
                     /locus_tag="IWJ80_002339"
     CDS             complement(4033..4455)
                     /gene="rplK"
                     /locus_tag="IWJ80_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP9462033.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4798..5466
                     /gene="sdaAB"
                     /locus_tag="IWJ80_002340"
     CDS             4798..5466
                     /gene="sdaAB"
                     /locus_tag="IWJ80_002340"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP9462034.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5479..6351
                     /gene="sdaAA"
                     /locus_tag="IWJ80_002341"
     CDS             5479..6351
                     /gene="sdaAA"
                     /locus_tag="IWJ80_002341"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP9462035.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6442..7461)
                     /locus_tag="IWJ80_002342"
     CDS             complement(6442..7461)
                     /locus_tag="IWJ80_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9462036.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7634..8521)
                     /locus_tag="IWJ80_002343"
     CDS             complement(7634..8521)
                     /locus_tag="IWJ80_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9462037.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8598..9143)
                     /gene="nusG"
                     /locus_tag="IWJ80_002344"
     CDS             complement(8598..9143)
                     /gene="nusG"
                     /locus_tag="IWJ80_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP9462038.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9244..9414)
                     /gene="secE"
                     /locus_tag="IWJ80_002345"
     CDS             complement(9244..9414)
                     /gene="secE"
                     /locus_tag="IWJ80_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP9462039.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9438..9590)
                     /gene="rpmG"
                     /locus_tag="IWJ80_002346"
     CDS             complement(9438..9590)
                     /gene="rpmG"
                     /locus_tag="IWJ80_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9462040.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9752..10396
                     /locus_tag="IWJ80_002347"
     CDS             9752..10396
                     /locus_tag="IWJ80_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9462041.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10452..10955)
                     /locus_tag="IWJ80_002348"
     CDS             complement(10452..10955)
                     /locus_tag="IWJ80_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9462042.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10968..11014)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         3362 a         2384 c         2099 g         3665 t
ORIGIN      
        1 tacagtatta tttcggttgc ttgctgcttc acctagccct tgtactcgtg tttgattggc
       61 gacttgacca aattgattcc cgacgtctaa taaaataata gctaaaagca aaatggccaa
      121 atggcttccg ccaaccaata aagcaataaa gcttattgtt tgcataacaa gtcccatata
      181 cacgattttt ctttcagaat aagaatctgc cagtcgtccc acgataggtg cagcaactgc
      241 tccagacaaa ccaaaaatcg ctaatatccc tgcttcgaat gttccccaat tgtaggcggg
      301 actgctgata taaaaaatca acgtagacca aaagatcgaa aatgttccaa acatgaaaaa
      361 tccactgact gccgcttctc ttaatagacg ttgagaagca actaaatgcg gcaaactttt
      421 aagtgaatca aggtatgaaa cagctgtact actttgctgg acagaacgcg gcattttcca
      481 gtaaagcaga agtgtaagaa ttccgactgc acataaagca aataaataga tcatccgcca
      541 agaaaaaaga ctggcaacaa taccagatac cgtccgagaa agcaaaatcc ccacgagtaa
      601 accgctaagc aatgtaccca tgaccttccc tcttgctttt ggtcccgcta gtaccgcacc
      661 gtaaggaatg atgatttgcg gcacgatcga aagtaatcct attaaaaaag aagcgcatgc
      721 aaataacgta aacgaaggcg caaaaaaagc ggccaacaga gagacggcag ataacgctac
      781 cattcgaatg atcaattttg gacgatccac cacgtccctt aaaggaacta aaaacagtaa
      841 ccctaacgca tagcctaatt gggtcaacat cgtcaatatc ccgactgcac ttgtgctaac
      901 agataaacta gctgctactt ttgttccaat cggctggatg taatacatat tggcaacaac
      961 aacgccacaa gtgattgata atagtaaaat aagcgaagtt gttaaaggtg ttttttcatt
     1021 actcttcatt gatttccccc atttcctatc ttagtttaca acttatggaa aagattggaa
     1081 atagaaaaat acaactattc gccacactta ttatttgtgc ttttgttttc cttgtttttt
     1141 cttgcgatct ttataaatga agacaactaa ccctactagc acagtaacac ttgcaaccaa
     1201 cgtgactgca gttttgttat atccgtgaaa cagagcttct ccaccacacg catacaacac
     1261 agacgaaggg atgactccaa tcacgatcga caatatgatt tgctgtagag gcaatttcat
     1321 cgcatctgca gcaaaattga ccatagaaga tggaataacc ggtatcatat agcctaacat
     1381 tacacctatt tttggatgtt tcatttgtcg gattgcttgc acccaatgat tcgtttcggt
     1441 tttcttatct aaaaatttta agcgatgcat aaggaaaatt gcagtgatgt taccaagagc
     1501 attacccaaa atgttgatga tcgcacctgt cagtggtcca aagctcaaac cagcaatgac
     1561 tccaactact gaagtaggaa tacctggaac agcacacatc accgctgtca atacgatcaa
     1621 gagtatcgat gcaaaagtcc catgcgaacg aatatgtgcg agtagctctg ccttactcgc
     1681 tctcggattt aaaaataatt ggatgtctgt ctgatattct aaatacaacc gatagcccac
     1741 taccagtatc agaatgattc ccaacgagat caacatcaat cggattattt tctttttcat
     1801 atttcctcct gaaaaaaatc tatattcatt aaataatagt ttaacataaa aacaaaaaag
     1861 caccgaaatc caacatagga tctcagtgct tttaaatagt aaaaattatt taacagttac
     1921 agatgcgcca acttcttcaa gtttagcttt caattcttca gcttcttctt tagatacgcc
     1981 ttctttaact ggtgcaggag cgccatcaac tacagcttta gcttctttca agcctaaacc
     2041 agttgcttca cgaactgctt tgataacttt aactttttgg tctccagctg cagttaattc
     2101 tacagtgaat tctgtttgtt cttcagcagc agcagcgcca ccagcagcag ctacagctac
     2161 aggagcagca gcagatacgc caaattcttc ttcaatagct ttaactaggt cgttaagttc
     2221 taggatagtt gcttctttta actcagcaac gatgttttca atgttcaatg ccattataat
     2281 ttcctccgtt taattgttct tttttattta atgctttatg cggcaattaa gctgcatctc
     2341 catcttcttt gtctgccact gctttgacag cgtaagccac gttgcggact ggcgcttgta
     2401 gtacagatag caacatagat agaagtcctt cgcgatttgg caattttgcc aatgctgtga
     2461 tttcttccaa tgaagagact ttaccttcga tgataccgcc tttgatttct aatgcttttg
     2521 cttctttagc aaattcgtcc atgattttcg ctggtgcgac tacatcttcg ttactgaatg
     2581 caacagctgt aggtccagtg aatacttcgt ccatgccttc aaggccagct tttttagctg
     2641 cacgtgaaag aatagaattt ttgataactt tcatttctac gttagcatca cgaagttgtt
     2701 tacgtaaacg tgttacttca tcaactgtta atccgcggta gtcaacgact actactgatg
     2761 cagctgctgt aaatttttcg cttacttcat caacgattgc tgcttttttt gcgataattg
     2821 cttcactcat ttttgagttt cacctcctga attttgatgg aagagttttt tgcactgaaa
     2881 aggcaagtca cttttatagt tactttgctc aaccagtatt attcatcacc tattcacttc
     2941 gttcatagtg attctactcg ctttgctcga aaggaaactc ctttcgcttt gctcaaccag
     3001 tattgttcat catctcttca cttcgttcat agtgattcac aataaaaaac tctatgccac
     3061 cgcagacata gagggactat tgaatatatt caataaacgt cctcggtagg aaattaaggc
     3121 ttgatgccac ctactgtctt cggtacaagt taattattaa cctcaactag cttagcatgc
     3181 taaactagtt gagtcaagaa ttatttttca gttggatcaa tcaacattct aatatttaat
     3241 tagaaagaag cttggtcaac atggatacca ggtccaaatg ttgttgtgat cgttacattt
     3301 ttcatgtatt gacctttagc tgctgatggt ttagctttta gcaatacatc atggattgct
     3361 gcaaagtttt caactaattt ttcatcgtca aatgaaactt ttccgattgg tacatggata
     3421 tttccagctt tgtccacacg gtaagttact ttaccagctt tcacttcgtt tactgctttt
     3481 gttacgtcca ttgtaactgt accagtttta gggtttggca ttaagccttt tggtcctaat
     3541 actcgtccta gacgacctac agtagccatc atatcaggtg ttgcaacaac tacatcaaag
     3601 tcaaaccatc cgttttggat tttttgaacc atgtcgtcgt cacctacgaa atctgcacca
     3661 gctgcttcag cttcttttgc tttgtctcct ttagcaaaaa ctaaaacagt ttgtgtttta
     3721 ccagttccgt ttggtaatac aacagcacca cggatttgtt ggtctgcttt tttagggtct
     3781 acgttcaaac ggtatgcaac ttcaactgtt gcatcaaatt ttgcaatgtt tgtttctttt
     3841 gctaaagcta ctgcttcggc aacaggataa actttacttg aatcaacttt ttttaatgca
     3901 tcttgcattt ttttgctctt tttagccatt ttggttcctc cttgattgtg gttataacgg
     3961 ttgtacctcc cacgtgttct atatctttca atatagagcc aactgagaag ctgctgtctt
     4021 agggtgtata gattattcta cagtgatccc catgcttcgt gcagttcctt caaccatgcg
     4081 cattgctgct tcaacgttag ctgcgtttag gtcttccatt tttaattcag cgatttctct
     4141 tacttgatcg ctagaaactt tcgcaacttt atttttgttt ggttcgcctg aacctttttc
     4201 gattttagct gcttttttca atagaacagc agctggcggt gtttttgtaa cgaatgtgaa
     4261 tgaacggtct tcgtatacag aaataacaac cggaatgatc aaacctgctt gatcagctgt
     4321 acgagcgttg aattcttttg tgaatcccat gatattgata cccgcttgac ctagtgcagg
     4381 acctactggt ggagctggag ttgctttacc tgcaggaatt tgcaatttaa cgattttttc
     4441 tactttcttt gccacgagac atacctcctt aagtccgtga tgtggttaat tggagatgca
     4501 tatttctcct cccacgtttt atctgatctg aaaaaatcca gacaaataat gtgcaaaaca
     4561 atgcacactg gtacagtata gcaaatttat ttgttttttc aagatttttt tctgctattt
     4621 ttacattttg cttgaataaa cgctaaattt attctaaaaa aacaacaatt tttcgttctc
     4681 atttcttctt ttatgttaca cttgcgctag catggttcaa cagatgaaag caaacaataa
     4741 cacggttttt tagacccttg tacaaagatt cctacataac aaaatgaggt ggagaaaatg
     4801 aaagatttaa ggtatagaag tgtctttgat attattggac ctgtgatgat tggtccaagt
     4861 agctctcaca ctgccggagc tgctagaatc gggaaaatca tacgtagtat atttggcgaa
     4921 cagcctgatt ctgtagatat ttatctatat gaatcatttg caaaaaccta tcgggggcac
     4981 ggaacagata ttgcattagt tggcggcctg ttagggatgg aacccgatga tgaacgttta
     5041 gctgattctt tgaaattggc atatgaagcc ggtatggaag tcttgtttgt tcctaagagt
     5101 gaaaaagccg aacatccaaa ctctgtaaag atgcttctaa aaaaaggaac acgaaagctt
     5161 tcagtcactg gcatttcgat cggcggtggc aatatccaga tttctgaatt gaatggattc
     5221 aaaatttctt taagcatggg tactcctacc ttgatcgttg tccaccaaga tgttccaggt
     5281 atgattgcta aagtgactag tatactttct gagaccaata cgaatatcgg cactatgaca
     5341 gtgacacggg aatcaaaagg tgaaaatgcc attatgataa ttgaagtaga tgatcctcaa
     5401 gtagaagaaa cggtaaaaag actcaaacag ctgccaaata tcgacagcgt aaattatttc
     5461 gattaaggag ctaaaaaaat gtttttatcg attgaagaat tagtagatca agcaaaagct
     5521 tatccaagtg tagcagaatt gatgattgca gttgaaatgg aaatgagtgg ccgttcccgt
     5581 agtcacatca tcgagatcat ggaaaaaaac ctctcagtaa tggaacaatc aatcaacgag
     5641 ggaaaagaag gtgttacctc cgtcacagga attaccggtg gtgatgcaaa aaaattagat
     5701 gattatttaa atgaggcaga ctttttaagt ggagaaacaa tcttaaaagc tgtacgcaat
     5761 gctgtagctg ttaatgaagt caatgcaaaa atggggttga tttgtgctac accgactgcc
     5821 ggaagcgcgg gtgttgttcc tggtgtgctg atggctgtcc gagatcggct tgatttgaca
     5881 catgaacagc aattggaatt cttatttacc gctggcgctt tcgggttagt catcgcaaac
     5941 aacgcttcta tcagtggtgc agaaggggga tgtcaagcag aagttggttc tgcaagtgca
     6001 atggcagcag cagctttggt ttgtgcaaaa aatgggactc ctcatcaagc agcacaagca
     6061 gtagccatta cattgaaaaa tatgatggga ctgatttgcg atcccgttgc aggcttagtt
     6121 gaagtcccgt gtgtcaaacg taatgcttta ggctcttctc aagcttttat ctctgctgat
     6181 atggcattag cgggtatcga aagtgtcatt ccgccggatg aagtcgttgc tgcaatgtac
     6241 caagtcggac gtcaaatgcc gtcgattttc aaagaaacag cagaaggtgg cttagctgtt
     6301 acgccaactg ctaaacgact tgcggaagag attttaaata agagggcgtg ataaagctcc
     6361 ttaatttcga gtattaagag attagttatc ttcataacaa aaaacgaaga gaggactaat
     6421 tgcccttctc ttcgtttttt attaatccat caatttcgta ttttttactt catcgatcgt
     6481 ttttgttcct gcaagttgca tcgtgatcga caattctttg ttcaggtgtt caaaaacaga
     6541 tgttactcct tctgccccac ctagattcaa cccataaatc actggtcggc caatcgcaac
     6601 taagtcagca ccacttgcca gagctttgaa gacatgctct cctcgacgga caccagagtc
     6661 gaaaatcact ggtacacgtt tgttgactac ttcggcaatt tttggcaata cttcaaagga
     6721 cgcaggacct ccatctaatt gacgtccacc atgattggat acccagatac catctgcccc
     6781 tgcagagata gcgattactg catcttctgg cgattgtatt ccttttacga taactggaag
     6841 atgggtgatt tctttgattg ttttgatgtc atctggtgtc aagccttgtt ttgcagctgc
     6901 ataaatttct gcgattcctt ttccttcgcc attaccactt gcactttgtt cgctatacgc
     6961 agctaagtta ggcattggta atggaaattg gaattgattg atgacgtctt cttcacggta
     7021 accaccgagc gttgaatctg ccgttaaaat gatagcttta gcccctgctt ctactgcttt
     7081 gttcaaaatg aactcattga atctgtcgtc tttactcata tataattgga aaaactgcgg
     7141 tgcgcctggc gctgcatcgg ctgcgtcttt gattgtcgta tttgcataag tgctgatgga
     7201 aaagattgat ccagcagcag ctactccttt agctgtatca gcttcccctt tttcatgtgc
     7261 caatccttgg gccgctgatg gcgcttggat aatcggtgta tctaattcaa ttccgaaaac
     7321 agatgtatgc aaatcggctg agtcgattcc gcgtaatatc cgtggcatga ttttctttgt
     7381 attaaaagaa gtcgtatttt ctttcatcgt ccactcatct tcagaaccac cgcgaatata
     7441 gccaaaagct cctgcttcca tgcgctcttt tacacgacct tctagttagg caatgtttac
     7501 aatatcgatt ggatgttctt cattacttgt atgataagtc atagtagatc cttctttcat
     7561 cttcttgatt acatataaat catactaaac ttacaaaaag taagcaaaca tttactctca
     7621 gatactttta tcgctattct ttccaaataa atcgacttac aagctgatct acttcttggt
     7681 tttcatgaag catactatga gaggcatttt cacctgtcac tactttttcc tctatttcat
     7741 ttccgcgttg cttcaacaac gcatagaccg ctaatgcact gtttaacggt actgttccat
     7801 cactcaagtc tgtttcatct agttgaccag caatcaagaa aaatcgagtg gttataggaa
     7861 tattaccgat catttgctgg taatcttgat agcggttaga aacgacagca ggcccctttg
     7921 ctaactcatc tgtcagagac tgcgcactat catcgacaaa atcattaaaa ggcgcgccaa
     7981 ttgctgttaa tgtctgaact tgtggcaatg acgtatcctg tccatacgtt cctaaatagc
     8041 gtagtgcact aactcctccc atggaatgcc ccactatgtt gactttatct atgccgtagt
     8101 tttctttcag atacaaaaga caagttttga tccattctgc ttgattccac tcgttatttt
     8161 tattatcttc aaacaatact tggaccatcg gattgtctgc ttttccagac aaatctcctg
     8221 ttgcttgtat ttgtccatct gttcctactg ttaaaaccaa ttcttttact gcttgctgat
     8281 ttttttccaa acgccgcaac atccctttaa aggaattttc tgtcccgctg tagccgtgga
     8341 aaaaaagtgt tggaatagtg gttttttcta tcactttact ggattctgcc gttttttctt
     8401 tatcagaaac ttcagtagta tttatcttcg tcgtctggca accacttaac gcaaacaaac
     8461 tgataaggac taaagctgct gcagttgcac gtttgcataa atttttgatt ggttctgtca
     8521 tacacctctt cctttccata tagaaaaaag aaagacttga aaggccaatc atatgatcga
     8581 catttcaagt ctctttatta atcgatttta tctacttgat cgaaatcaag ttctgtactg
     8641 gtttcacgtc caaacatatc gatgttgact ttcaattttt ctttttctcc gtcaacttct
     8701 gttacttgtc cttctaatcc agcaaatgcg ccttcgatga ttttcacgac ttcgccaact
     8761 tctacttcta gatcattttg tcgcaggctc ataccgatag aacgcaagat atgattgact
     8821 tcttctggaa gtaatggtgc tggtttgctt ccggctccgt gtgaaccaac gaatcctgtc
     8881 acaccaggtg tgttgcggac aacataccat gaagcgtctg tcatgatcat ttcaacaagt
     8941 acgtatcctg ggaatgtttt atgaacgatt tctttttctt tcccattttt gacttcagtt
     9001 tctttttctt caggaacaac gacacggaaa ataaagtcgg ccattttcat actttgcgca
     9061 cgagattcga tatttgcttt tactttgttt tcatatcctg aatatgtatg caagacatac
     9121 cagttttttt caaaagattc cattgaaggc tcctcatttc attgtaaaat aaaaaaacct
     9181 cagtacaccc gaagtttttc tttcgcttca ttatagcact aaaagtacgt tgtggctagc
     9241 gagttatttt aaaatccagc caaataagct ttggatggct gtatccatta cgtaaaacat
     9301 caatccgaaa ataatagaag tttcgatgac tactaacgta tctttacgga gttgttttct
     9361 gcttggccaa gttacttgtt tgatttcatc ttttacgctt tttagaaatt tcatgagaca
     9421 acctcccttg ctactgttta tttcgtttct ttatgtaatg tatattgatt acaatattta
     9481 caaaacttat tgatttccaa acgttcgccg ctttttcctt cactgactga ttttgtgtag
     9541 ttgcgagacc cacaaacgga acaagcgagt gctgcttttt tctttgccat ttacttctgc
     9601 ccccttgctg agaatcagac ttatacttca taacagtatc acgtaacttt ttacttgtca
     9661 atttgtgaaa taaactatct attgttttgg ataaaattat gatatgatta atttgaaatt
     9721 aagctaaaat gagacaattg gaggtatagc gatgctatta aaaacacttg tatataagaa
     9781 aaaagatctg acaaccgtta cagagactag tacattagaa gaagcattaa aaattttaga
     9841 aacttccgga taccgttgtg tgccgatctt agattcttca ggtaatattt tccgtggaaa
     9901 catttacaaa atgcatatct atcgtcataa agcaaatggc ggtgatatga cgcttcctgt
     9961 cactcatctt ttgaaaaacg ctacaaagtt catttacctg gatacttctt tcttcaaagt
    10021 attctttacg atcaaagagc ttccttatat tgctgtcctt gacagtgaaa atcattttta
    10081 tggtattttg acacatagta cgttgcttaa tatgctctct caatcttgga gcgtagaaca
    10141 aggaagctat gtactgacaa ttgcttccac aggaaagcaa ggagatttgg ctgctattgc
    10201 aaaaatcatt tcaaaacaca gcagcattgc cagctgtatt actcttgata tcgaaaaaga
    10261 cgaatacatt cgacgtacat tgatcacgct accggctaac actgaagcga aagtatgtga
    10321 aacgatcgtc aaccacttag agaaaaagaa ttataaagtg gtagaaattg aaaatttgca
    10381 aaaccaagaa gcataatcaa aaagcgaaaa aagaaaacag ctaatctgct ttcttttttc
    10441 gctttttata attagacacg cttttcaaaa tcaagcgcgc gatctaaata atacatcaat
    10501 ggtgctgaaa cagccatgac gataaactca cttaatgcaa gcgtgccata ggttgtccaa
    10561 aatgcttggc cacctgttgg aactaacata taagcaatca gtcccatgct gatggtaaag
    10621 aagatcgtgt tcaatactaa acgcatcttt acatttttca ctttgttttg caataaagct
    10681 gtcaggccta aagaaagcaa tgtttggccg ccaccaaaaa ccacatccat gataccaaaa
    10741 ccaaagaagg cattatagac gatgaccccg cctaaaactc cccaaagcaa tttacggtta
    10801 aagacaacta aatgattcaa actttctgaa atacgaaact ggatcggccc tgaggataca
    10861 ggcgcaacta aaatagttaa cgctagataa agagccatga tgatgccatt tacaacgatt
    10921 acggataacc gttgtctgct tttcgttttt tgcattttct ctcctctttt ctcctagttt
    10981 ttttaacgcg ggatggttga tgaaccgcgg ttatgtgaaa caaacaataa ttataacaga
    11041 cttttcattt atttcaatcc gtcttatctg cttttaaaaa agcaaaagct taaacaatgt
    11101 aaacgtttgc taaaaatcgt gggatatggt ataaagtaag tgtaaggggt cataagaatt
    11161 cccagtaaag cagacgaagt aggatctgca agaacagaat tgttgtgaaa cttaaataaa
    11221 taaaaaagga tgtgggaatt tgagcaacct cttactagag aacggtctag caagcagctg
    11281 aagatctaag tggacatgcc gaaccttgga acttctcctt acgtaagtaa tggatcatgc
    11341 gcttgctagg cttttttgtg tttttcggtc ttcggacctt tttcattttc ctagacttct
    11401 gctataataa ccaagaatta attttgtaac agattttttt gaaaaaacaa gtttctttca
    11461 ttcaaaaaat agaaaaagct tgctaattat tggttaacca gcaaacagga
//
LOCUS       DACDRN010000078        11274 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDRN010000078 DACDRN010000000
VERSION     DACDRN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.078"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..683
                     /locus_tag="IWJ80_002349"
     CDS             <1..683
                     /locus_tag="IWJ80_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HAP9462043.1"
                     /translation="GIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLN
                     RKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFS
                     HIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLK
                     VEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELG
                     CANASEAV"
     gene            complement(713..1471)
                     /locus_tag="IWJ80_002350"
     CDS             complement(713..1471)
                     /locus_tag="IWJ80_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9462044.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RCWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRKLQNIGAYQYFEKQNIIISQSTHGGA
                     AENSYEGNVKNLQSYQNVQLSKRAIEILKEEIELNHQHIRFNPDYKDNGWIFTSKSRH
                     KPGMATVFLTNFIRCRTSFRMLFPIVLDNEPPRM"
     gene            complement(1476..2243)
                     /locus_tag="IWJ80_002351"
     CDS             complement(1476..2243)
                     /locus_tag="IWJ80_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9462045.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            2732..3157
                     /gene="lacA"
                     /locus_tag="IWJ80_002352"
     CDS             2732..3157
                     /gene="lacA"
                     /locus_tag="IWJ80_002352"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP9462046.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            3174..3689
                     /gene="lacB"
                     /locus_tag="IWJ80_002353"
     CDS             3174..3689
                     /gene="lacB"
                     /locus_tag="IWJ80_002353"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP9462047.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            3700..4632
                     /gene="lacC"
                     /locus_tag="IWJ80_002354"
     CDS             3700..4632
                     /gene="lacC"
                     /locus_tag="IWJ80_002354"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP9462048.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            4635..5615
                     /gene="lacD"
                     /locus_tag="IWJ80_002355"
     CDS             4635..5615
                     /gene="lacD"
                     /locus_tag="IWJ80_002355"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9462049.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            5643..5960
                     /locus_tag="IWJ80_002356"
     CDS             5643..5960
                     /locus_tag="IWJ80_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9462050.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            5960..7666
                     /locus_tag="IWJ80_002357"
     CDS             5960..7666
                     /locus_tag="IWJ80_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9462051.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            7808..9214
                     /gene="lacG"
                     /locus_tag="IWJ80_002358"
     CDS             7808..9214
                     /gene="lacG"
                     /locus_tag="IWJ80_002358"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9462052.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRLKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            9237..9510
                     /locus_tag="IWJ80_002359"
                     /pseudo
     CDS             9237..9510
                     /locus_tag="IWJ80_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
     gene            9531..10430
                     /locus_tag="IWJ80_002360"
     CDS             9531..10430
                     /locus_tag="IWJ80_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP9462053.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDEDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSSLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
     gene            complement(10902..>11274)
                     /locus_tag="IWJ80_002361"
     CDS             complement(10902..>11274)
                     /locus_tag="IWJ80_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9462054.1"
                     /translation="IKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPF
                     DNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRN
                     QRLAQRILDNELGCANASEAV"
BASE COUNT         3550 a         1931 c         2260 g         3533 t
ORIGIN      
        1 gaggcattca gatcagccga cgaaaaatta gtagaatcat gaaaaataga ggattaaaat
       61 cgagctatac tgttgcttat tttaaagtac atcattctac ttgcaatgaa gccaaaacga
      121 caaacgtatt gaatcgtaaa ttcttaagag acaacccatt agaagcgatc gtaacagact
      181 tgacttatgt acgagtcggg aaaaaatgga attatgtctg tttcattttg gatttgttca
      241 atcgagaaat tctcggctat tcttgtggag aacataaaga tgccgttcta gtaaaaaaag
      301 catttagcca tatcaaacaa cctctgacag aggttgagat ttttcatact gatcgtggaa
      361 aagagtttga taaccaagct attgatgaat tattaacaac ttttgacatc aatcgatcat
      421 tgagtcataa aggctgtcct tttgataatg ccgtagctga atcaacttat aagtcgctga
      481 aagtagaatt tgtctatcaa tacacatttg aaaccttaca acaattggat ttggagttat
      541 ttgactatgt caattggtgg aaccaccttc ggttgcacgg tacacttggc tacgagacac
      601 cggttggtta ccgtaaccag agattggcgc agcgaatcct tgataatgag ctcggatgtg
      661 ctaacgctag cgaggcagtc taactttaac tgttagctcc tgccgaagat cgtcacatcc
      721 gaggaggctc attgtcaagg acaatcggga atagcatacg gaaagaagtt ctgcacctta
      781 taaaatttgt caaaaaaact gttgccattc caggtttatg acgacttttg gaagtaaaaa
      841 tccatccatt atctttataa tcaggattga atcttatatg ttggtgattt aactcaattt
      901 cctctttaag aatttctata gcccttttag ataattggac attttggtaa ctttgtaagt
      961 tttttacatt accttcatag ctattttctg cagctcctcc gtgagtggac tgactaatga
     1021 tgatattttg cttttcaaaa tattgatagg caccaatatt ttggagtttt cgactctgta
     1081 agtcaacagg gccattagtt agaataccta ttgggatgcg gtttttatgt agagccagca
     1141 gtagttgatg tatctcagga atcatcgtaa tctgatcgag ttcatattca tagtcagctt
     1201 ggaagtctag agcttctgtc ctagaaatac tatcgatgcc aaattcttcc atagtcttct
     1261 gacagcgaaa aattctaagc tgctcaatac tgatgagttt tttcgtgtat ttaggaaaag
     1321 caacatcaga ccaacatctg aaacgtttgt aaatgttatc tatttgttgg atatcaatcg
     1381 ttggaaaaca tttctcaacg ctacgtttaa aggggagttg atggtcataa agagtgtcat
     1441 caaggtcaaa aacaatattt ttataagtca taattctagt ccgcttttaa aatagtagtg
     1501 tactggctaa attcctctaa gtgttgaggg ctaatctggt tatctgtaat gatggtatcg
     1561 agttgatcta gattatagaa gttaaagaaa tcgtatcgat cgaatttagt actgtctact
     1621 aataagaatt tttctacagc attgtttagg gcaagttgtt gaatcacacc ttccttgtca
     1681 ctatatgtag caatagaatt atgggtaaca gcatttgcac taacaaaagc tttggcaaat
     1741 ctaattgctt ttaaatttgt cgaagccatt gaacctacaa aagctccagt tatttctcta
     1801 tattcaccgc caagaagcaa taaatcaatg gtttcgctat catttagaat caagaacaca
     1861 gggagactat ttgtaatgac acgaatttta taacctttac gacctttcaa ttctaatgcc
     1921 agttgttcaa gtgtagttcc gggtccaata aaaatagtat cgccatcttt gattaaagag
     1981 caggcttttt tagcaatgtc aattttttct tttgtattta gacttttctt ctcgatatgt
     2041 gtcttttcaa gtggcttttt agaggaaagt aattgtgcac caccatgagt acgtgttaaa
     2101 agtccatcag cttctagagc atcaaggtct ctacgggctg tcatatcgga aatatctagt
     2161 tcatctatta tttcttttat ggttatggtc ccatcaatct ttaacatatc taaaattttt
     2221 tctaatcgtc gttttttgtt catatgaaga cccttctttc ataaagtaat ttttttccaa
     2281 agataattct cttttaattg tatcataaaa gacaatattt tcaaggtaaa acaaacaatt
     2341 tcaaacaaaa acaaatgtta gatgatgaaa taagaacagt ggattgacgt atattagctt
     2401 aggtcagatt ttgtataaga cgaaaataaa gtaggacctc ttaatcagta agttatagaa
     2461 agtaaaagac ttttgtaata cttgaataga tatttcacgt ccattttgtg atggattaaa
     2521 tgaacaaaaa tgaacaataa tttaacggtg ttatctattt tttaagaaaa caaataaaaa
     2581 aaacaaaaaa taaacaaaaa agtgttgctt tttgtttgaa tgattgatat tatataaaca
     2641 aagaaatgat gaaaacgttt tttcgaacat tttgcaaaat gttttctact tctacgtagc
     2701 atttctttct aaaatttagg aggtggtcaa aatggctatt gttgttggtg cagatctcaa
     2761 aggtacgaga ttaaaagatg ttgtgaaaaa ttttcttgta gaagaaggtt ttgaggtaat
     2821 tgatgtgact aaggacggac aagattttgt tgatgttacc ttggcagttg cctctgaagt
     2881 aaataaagat gagcaaaact taggtattgt aattgatgct tatggagctg gtccattcat
     2941 ggtagcaact aaaattaaag gtatggttgc agcagaagtt tcagatgaac gctcagctta
     3001 tatgacacgt ggacataaca attcacgaat gattacggtt ggcgctgaaa tcgtcggtga
     3061 tgagcttgct aaaaacatcg ctaaaggttt cgtcaatggt aaatatgatg gcggacgtca
     3121 tcaaatccga gtagatatgc ttaacaaaat gtgttaagaa aaggagttaa ggaatgagaa
     3181 tcgcaattgg atgtgaccac attgtcacag atgaaaaaat ggcagtatca gaattcttga
     3241 aatcaaaagg acatgaggtc cttgatttcg ggacatatga ccacgcacgt actcactacc
     3301 ctatctatgg taaaaaagtc ggtgaagcag tagtaagtgg tcaagcagac ttgggagtat
     3361 gtatctgtgg tacaggtgtc ggcattaaca atgcagtcaa taaagttcca ggtgttcgct
     3421 cagcgttggt tcgtgatatg acatcagcta tatatgcaaa agaagagtta aatgcgaatg
     3481 ttatcggttt cggtggaaag attactggtg gtcttcttat gaatgacatc attgaagctt
     3541 tcattgaagc tgagtacaaa ccaacagaag aaaataaaaa attgattgca aaaatcgaac
     3601 acgttgaaac acataatgca catcaagcag atgaagattt ctttacagaa ttccttgaaa
     3661 gatgggaccg tggtgactac cacgattaag gtataacaaa tgattctgac agtcacacta
     3721 aatccttcag tagacatctc ttatcctcta gaaacattga aaattgatac agtgaatagg
     3781 gtgaaagatg ttagtaagac agctggtgga aaaggcttaa atgttactag agtcttgtat
     3841 gaatctggtg ataaagttac tgctacaggt ttcttaggtg gtaaaattgg tgaatttatt
     3901 gaaagcgaat tagaacaatc tcctgttagt ccagcttttt ataaaatctc aggaaacact
     3961 agaaactgta ttgctatctt acacgagggt aaccaaacag aaattttgga acaaggacca
     4021 acaatttctc atgaagaagc agaaggcttt cttgaccact atagcaatct tattaaacaa
     4081 tcagaagttg tgacaatttc aggaagttta ccttcaggac ttcccaacga ttattatgaa
     4141 aaacttatcc aacttgcttc ggatgaagga gtagcagtcg ttttggattg ttcaggtgca
     4201 cctcttgaaa cagttctaaa atcgtcggct aaacctactg ccatcaaacc taataatgag
     4261 gaactttcac agttgcttgg aaaagaagta acaaaagata ttgaagaact caaagacgtt
     4321 cttaaagagt cactttttag tggtatcgaa tggatagtgg tctcattagg tcgaaacggt
     4381 gcctttgcaa aacatggtga tgttttctat aaggtagata ttcctgatat cccagttgtt
     4441 aatccagtcg gatcagggga ctcaacggta gctggtattg catcagcttt aaatagtaaa
     4501 aaaagtgacg ctgacttatt aaaacatgcg atgacattgg gtatgttaaa tgctcaagaa
     4561 acaatgacag ggcatgtgaa tatgactaat tacgaaacac taaactcaca aattggagta
     4621 aaagaggtat aaaaatggta cttacagaac agaaacgcaa atctttggaa aaaatttcag
     4681 ataaaaacgg tgttatctca gctttggcat ttgaccaacg tggtgctttg aaacgtttga
     4741 tggcacagta tcaagataca gaaccaactg tagctcaaat ggaagaacta aaggttttgg
     4801 ttgctgatga acttacaaaa tacgcttctt caatgttgct tgaccctgaa tatggacttc
     4861 cagcaacaaa agcattggat aaagaagcag gtcttcttct tgcctatgaa aaaacaggtt
     4921 atgatacatc aagtacaaaa cgtttgcctg actgcttgga tgtttggtct gcaaaacgta
     4981 tcaaagagca aggcgctgat gccgttaagt tcttgcttta ctatgatgta gacagctcag
     5041 atgaacttaa ccaacaaaaa caagcttata ttgaacgtgt tggttctgaa tgtgtcgctg
     5101 aagatattcc tttcttcttg gaaatccttg cttatgatga agaaatctca gatgcaggtt
     5161 ctgttgaata tgcaaaagtg aaacctcgca aagtcattga agcgatgaaa gttttctctg
     5221 atccacgctt taacattgat gttcttaaag tagaagtacc agttaacgtt aaatatgtcg
     5281 aaggttttgc tgatggtgaa gtggtttaca gtaaagctga agctgctgac ttctttaaag
     5341 cacaagaaga agcgactaac cttccataca tctacctaag tgcaggtgta tctgctaaat
     5401 tgttccaaga aacattgcaa tttgctcacg actcaggtgc caagtttaat ggtgtgcttt
     5461 gtggacgtgc tacttgggca ggatctgtag aaccttacat caaagaaggt gaaaaagcag
     5521 cacgtgaatg gttgcgtact actggattcg aaaatattga tgaacttaac aaagttcttg
     5581 ttaaaacagc tagtccatgg actgataaag tatagtaaaa acataaaacg gaggatattg
     5641 ttatgaacag agaagagatg actctcttag ggtttgaaat tgttgcttat gctggagatg
     5701 ctcgctctaa acttttagaa gcgcttaaag cggctgaaaa tggtgatttc gctaaggcag
     5761 atagtcttgt agtagaagca ggaagctgta ttgcagaggc tcacaattct cagacagcta
     5821 tgttggctcg agaagcttct ggggaggaac ttccatacag cgttaccatg atgcatggtc
     5881 aggaccactt aatgactact gtcttattaa aagatgtgat tcatcacctc atcgaacttt
     5941 ataaaagagg agcaaaataa tgcataaact cattgaactt attgagaaag ggaaaccgtt
     6001 ctttgagaaa atctctcgaa acatctatct tcgtgctatt cgtgatggat ttatcgctgg
     6061 tatgccagtc atccttttct catctatctt tatccttatt gcctatgtcc caaatgcttg
     6121 gggattccac tggtcaaaag acattgagac cttcttgatg actccttata gctattcgat
     6181 gggtatccta gcattctttg ttggtggtac taccgctaaa gctttgacgg actcaatgaa
     6241 ccgtgaccta cctgcgacca atcagattaa cttcttatct acgatgctag cgtctatggt
     6301 agggttcctt ttgatggcag ctgagcctgc aaaagaagga ggcttcttga cagccttcac
     6361 gggaacaaaa ggtcttttga cagcatttat tgcagccttt gtgacagtta atgtttacaa
     6421 ggtctgtgtg aaaaataatg ttactattcg tatgcctgaa gaggttccac caaatatctc
     6481 tcaagtattt aaagacttga ttccattcac tgtagcggtt gttctccttt atggatttga
     6541 actcattgtt aagggaactc ttggtgtaac cgttgcagaa tcaatcggta cccttcttgc
     6601 tcctctcttc tcagctgcag atggttacct tggaattaca cttatctttg gtgcttatgc
     6661 cttcttctgg tttgttggta tccacggtcc ttcaattgtc gaaccagcaa tcgctgctat
     6721 cacttatgcc aatatcgatg ccaacttgca tcttatccaa gctggacaac atgcagataa
     6781 agttatcact tctggtactc aaatgtttat tgttaccatg ggtggaacag gagctacatt
     6841 gattgttcca ttcttgttca tgtggatttg taaatcagaa cgtaaccgtg ccatcggacg
     6901 tgcctcagtt gttccaacat tctttggggt taatgagcca atcttatttg gtgcaccaat
     6961 cgtattgaat ccgattttct ttgtaccgtt cattttcgct ccgattgtca acgtttggat
     7021 ctttaaattc tttgttgaca cattgaacat gaactcattc tctgccaacc ttccatgggt
     7081 tactcctggt ccgttgggaa ttgtactcgg tactaacttc caagttctat catttatctt
     7141 agccggactc ttggtagttg ttgatactat catttattac ccatttgtta aggtatacga
     7201 tgaacaaatt cttgaagaag aacgttctgg taaaacaaac gatgctctta aagaaaaagt
     7261 agctgcaaac ttcaatactg ctaaagcaga tgccgttctt ggaaaagcag gcgttgctaa
     7321 agaagatgta gcagcaaaca ataatatcac aaaagaaact aatgtccttg ttctttgtgc
     7381 aggtggaggt acaagtggct tgcttgccaa tgccttgaac aaagcagcag cagaatacaa
     7441 tgttccagtc aaagcggcag cgggtagtta cggtgctcac cgtgaaatgt tgccagagtt
     7501 tgacttggtt atcttggctc cgcaggtcgc ttcaaacttc gatgatatga aggctgaaac
     7561 ggataaattg ggcattaaac ttgcaaaaac tgaaggagct caatatatca aattgacacg
     7621 tgatggacaa ggtgctcttg catttgttca acaacagttt gattaataaa tttgtaaaag
     7681 ttatttgaga cgagattatt ctttcagttc cttataatat tgttaaccat atcattgcat
     7741 ttcatttcct tggtttccgg aactcagaat atcgtctcaa atgctttttt tgaaaggatt
     7801 tatagttatg actaaaacac ttcctaaaga ttttattttt ggtggtgcta cagctgctta
     7861 tcaagcagaa ggtgcgactc acacggatgg taaaggccca gttgcatggg ataagtatct
     7921 tgaagacaat tattggtaca cagcagagcc tgcaagtgat ttttaccata aatatcctgt
     7981 tgatttagaa ttagctgaaa aatatggcgt taatggtatt cgtatttcaa ttgcctggtc
     8041 tcgtattttc ccaactggct atggcgaagt aaatgaaaaa ggtgttgaat tctaccataa
     8101 tctttttgcc gagtgccaca aacgtcatgt tgagccattc gtgactttgc accactttga
     8161 cacaccagaa gttcttcact cagatggaga tttcttaaac cgcgaaaata tagaacactt
     8221 tgtagattat gccgctttct gttttgaaga gttcccagaa gtaaactact ggacaacttt
     8281 caatgaaatt ggcccaattg gagatggtca atacttggtt ggtaaattcc ctccaggcat
     8341 tcaatatgat cttgccaaag tcttccaatc acaccacaat atgatggttt ctcatgcacg
     8401 tgctgtgaaa ttgtataaag ataaaggtta taaaggcgaa attggtgtag tacatgcgtt
     8461 gccaaccaaa tacccttatg atccagaaaa tccagcagat gttcgtgccg ctgagttaga
     8521 agatatcatc cataacaaat ttatcttgga tgcaacctac cttggacact attcagataa
     8581 aacgatggaa ggggtcaacc acatcctagc tgaaaatggt ggagaacttg atcttcgtga
     8641 tgaagacttc caagcacttg aagcagctaa agatttgaac gatttccttg gtatcaacta
     8701 ctacatgagt gattggatgc aagcctttga tggtgagact gaaatcattc acaatggtaa
     8761 gggtgaaaaa ggaagttcta agtaccagat taaaggtgtt ggtcgccgaa tcgcaccaga
     8821 ttatgtgcct agaacggatt gggactggat tatttatcct gaaggcttgt atgaccaaat
     8881 catgcgattg aaaaatgatt atccaaacta caagaagatt tacatcactg aaaacggtct
     8941 tggatacaaa gatgagtttg tagataatac cgtttatgat gatggtcgta ttgattacgt
     9001 gaagaaacac ttggaagttt tatcagacgc aattgcggat ggtgcaaatg ttaaaggtta
     9061 cttcatctgg tcacttatgg atgtcttctc atggtcaaac ggatacgaaa aacgttatgg
     9121 actattctat gtagactttg acacgcaaga acgctatcct aagaaatcag ctcattggta
     9181 taagaaagta gcagaaactc aagtgataga gtaatattac ttgaaaatgt aaaaaattgt
     9241 tatccgatac tttagaaaaa gtacaacact tatttagacc aggctttgaa aaggtatata
     9301 ttgttagttt cgaagattgt cctatgattc ctgagcttga agctaccctc gttgctaaaa
     9361 tgtggtaaat ggtatgtttt aactggaaaa gagtggattt gccattctga tttagagtta
     9421 tcagcatttg aatgggaatt tttacaatca ctcgatgtag aaatacgtga gactattcac
     9481 ttcgaggtaa attatttgcc tttccaataa taaaaagaaa ggaattagtt gtgactatcg
     9541 aattaaaaaa cgagtatctt actgttcagt ttaaaacact tggtggtcaa ctgacttcga
     9601 ttaaagacga ggatgggata gagtatctct ggcaagctga tccaaattat tggaatggac
     9661 aggcgcctat tctatttcct atctgtggta gtttacgaaa tgattgggct atttatagac
     9721 ctcaagaaag accttttttt acaggtctta ttcgtagaca tggttttgtt cgaaaagaag
     9781 aatttattct ggaagaagtc aatgataata gcgtgacctt tagtattaaa ccaaatgctg
     9841 agatgcttga taattaccta tatcagtttg aactgagagt tgtttatacc ttgaatggta
     9901 aatcaattag aaccgaattt caggtaacga acttggaaat ggaaaaaaca atgccttact
     9961 ttattggagc ccatccagca ttcaatagtt ctttagtaga aggtgaaaac tatgaggatt
    10021 attatattga atttagtgag gttgagtctt gctcgatacc aaagagtttt cctgagacag
    10081 gattattaga tttgcaagat cggacacctt ttttggaaaa tcaaaaaatt ctcgatttgg
    10141 actactcact tttttcccat gatgccatta cacttgacca gctcaagtct cgtagcgtaa
    10201 cgcttcggtc aaggaagtct ggtaaaggtc ttcgagtaga ttttgatgat tttccgaact
    10261 tgattctgtg gtctacaact aataaaagtc cattcattgc tttggaacct tggagtggtc
    10321 tctccacttc acttgaagaa ggtaatttct tagaagataa aagacaggta acgaaaattt
    10381 cacctcaaga aacttctaga aaaatctatg atattactat ccttaattaa aatttgttat
    10441 tatttgagtc ttcaataaga tttgaatact cttaccttgt gatgatagat tggtcttctc
    10501 cgtgccagtc ttacatcgca aggtttttaa gttttttgtg ggtctatgtc aactgtagtg
    10561 ggtgacgaaa agctaatatc taaagacagt ctggtagtaa aaaataagat tatttactac
    10621 ttcttgattt aaaataatag tggagccatt aatttgtggc aaatttaatt taatattttg
    10681 cctcaaatta gccacacttt ttatagaact aagaggaaaa atgaaatttt cttcctatta
    10741 atatagttgt ttgaaatctt aaatatagcg ttttggaatg gcaacagttt ttttgacaaa
    10801 ttttataagg tgcagaactt ctttccgtat gctattccga ttgtccttga caatgagcct
    10861 cctcggatgt gacgatcttc ggcaggagct aacagttaaa gttagactgc ctcgctagcg
    10921 ttagcacatc cgagctcatt atcaaggatt cgctgcgcca atctctggtt acggtaacca
    10981 accggtgtct cgtagccaag tgtaccgtgc aaccgaaggt ggttccacca atttacatag
    11041 tcaaataact ccaaatccaa ttgttgtaag gtttcaaatg tgtattgata gacaaattct
    11101 actttcaacg acttataagt tgattcagct acggcattat caaaaggaca gcctttatga
    11161 ctcaatgatc gattgatgtc aaaagttgtt aataattcat caatagcttg gttatcaaac
    11221 tcttttccac gatcagtatg aaaaatctca acctctgtca gaggttgttt gata
//
LOCUS       DACDRN010000079        10773 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDRN010000079 DACDRN010000000
VERSION     DACDRN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.079"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            23..646
                     /locus_tag="IWJ80_002362"
     CDS             23..646
                     /locus_tag="IWJ80_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462055.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IWJ80_002363"
     CDS             1096..2145
                     /locus_tag="IWJ80_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9462056.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IWJ80_002364"
     CDS             2142..3023
                     /locus_tag="IWJ80_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9462057.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="IWJ80_002365"
     CDS             3026..3796
                     /locus_tag="IWJ80_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9462058.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="IWJ80_002366"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="IWJ80_002366"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP9462059.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="IWJ80_002367"
     CDS             5107..6270
                     /locus_tag="IWJ80_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9462060.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="IWJ80_002368"
     CDS             6387..6797
                     /locus_tag="IWJ80_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9462061.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="IWJ80_002369"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="IWJ80_002369"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9462062.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9337..9654
                     /locus_tag="IWJ80_002370"
     CDS             9337..9654
                     /locus_tag="IWJ80_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462063.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9814..10050
                     /locus_tag="IWJ80_002371"
     CDS             9814..10050
                     /locus_tag="IWJ80_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9462064.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="IWJ80_002372"
     CDS             10047..10445
                     /locus_tag="IWJ80_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9462065.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3768 a         1790 c         2263 g         2952 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag
    10681 tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc gtcatttttc
    10741 catcaaaatc tgcttatgca aaattataaa aaa
//
LOCUS       DACDRN010000080        10575 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDRN010000080 DACDRN010000000
VERSION     DACDRN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.080"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(47..1243)
                     /locus_tag="IWJ80_002373"
     CDS             complement(47..1243)
                     /locus_tag="IWJ80_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP9462066.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(1367..2029)
                     /locus_tag="IWJ80_002374"
     CDS             complement(1367..2029)
                     /locus_tag="IWJ80_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP9462067.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            2257..2820
                     /locus_tag="IWJ80_002375"
     CDS             2257..2820
                     /locus_tag="IWJ80_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9462068.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2833..3117
                     /locus_tag="IWJ80_002376"
     CDS             2833..3117
                     /locus_tag="IWJ80_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462069.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            3136..3828
                     /locus_tag="IWJ80_002377"
     CDS             3136..3828
                     /locus_tag="IWJ80_002377"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP9462070.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            3840..4385
                     /locus_tag="IWJ80_002378"
     CDS             3840..4385
                     /locus_tag="IWJ80_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9462071.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            4656..7934
                     /locus_tag="IWJ80_002379"
     CDS             4656..7934
                     /locus_tag="IWJ80_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP9462072.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8475..10382)
                     /locus_tag="IWJ80_002380"
     CDS             complement(8475..10382)
                     /locus_tag="IWJ80_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP9462073.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
BASE COUNT         3286 a         1961 c         2176 g         3152 t
ORIGIN      
        1 ataaactgtt gttgggcgtt tgttttagta acaagcgaaa ggtgatctat tgggttaaat
       61 ccaacagttt ttgtttcaga tcttcttcca tgtgtccaag ctctacttct gcatgacgac
      121 gtttttcttt tccttctttt tggatacgca atgtctcttg gattgtttcg atcaaatcat
      181 tttgagtggt ttgaagggtc tcgatatcca cgattccacg ttcattttct ttcgctgttt
      241 cgatggctga gattttcagc atttctgaat ttttcttcaa taagtcgttt gttgtttcag
      301 agacttggcg ttgagcagtt actgcatctt tttgacgaag caaggtcaat gcgatcacga
      361 cttggttttt ccacagcgga atggctgtag cgatagatgc ttggatcttt tcagccaagg
      421 cctgatttgt gttttggatc aaacggattt gtggtgcttg ctggatcgtg atctgtctag
      481 ctaagcgaag atcatgcgta cgtttatcca gacgatctag gaattgcgtg taatcattgg
      541 caatctgaac atccatctga tcgccagttt cttctgctct tttcatcgct tctggaatga
      601 tcgttgtttg aagttcttcc attttcaatt ctccagcagc gatataaata ttcagtgcat
      661 caaagtagtc tttgtttttc tggtaaagct gttctaacat cagattatct tttagcagac
      721 cgtctttttc tttatctaat ttcactgaaa tcttgtcaat ttgtgcacca atcttttgat
      781 attttgctgt cacttcatag atcgattgtt tgaccttccc gaagacacgc tggaagatgt
      841 tcccttctcc ggcacgcagt tcgtcaggat tggcttcttg gagacgatac atcagttcag
      901 ttaaggaatc tcctacagga ccaatatctt gtgcttgcac atgattcaac atcgattgtg
      961 agaattcact taattttgct tgggcagccg atccgtagct gatgacagac tgtgcatcat
     1021 tagcatcgat ttttgctgct agctgtttag cttgttcttg gcgttccgcc ggtagtttgt
     1081 caattaaacg agcagctgtc tgttgctctt ggagctgatc aatctccgct tgttgtgaag
     1141 tggtcagcgt atctgttgga gttgaaaacg ggttgttcaa taaatcttca agtgtatcgt
     1201 taactggtgt aacatctttt gtttcagtcg gtttatgatc cataaaaaat tcctccttgt
     1261 tgaatctgtt ctcgattctt acatgaatgt ctctacttta gaaaaagtac tcgaaacagc
     1321 cagacatcat ctgaatgttc cgagtcttct tttatgaaca aaagagttaa tccttttgtt
     1381 ctggttcata tttattatca cgctttaagc tctgtttcgc aacagatatc tctacgtcga
     1441 tatcatctag atcctcagca acgaattttt gataatcttg tgcgattaaa gctgacagct
     1501 ggtcgatgat ctgcgcgctt tcttctagtt tttcataggt ctgtttgttt ttgatctcat
     1561 ggtcattgat ctcattatac ttatttgtca aatcaactag attcggcaga tgcgtataaa
     1621 gaaactgact tgcttcatgc agtcggtttg gttctttcac caattctttg aataaagctt
     1681 tcgctgcttt gactggatca tggcggagat cgattgcttt aagtttggct gtctgctgca
     1741 tattcatttg caattgattg atctcatttt ttgtttgatt cattgtttgt cgaaaaaatg
     1801 aaatttcatt cgctgtcata ccgctttttt catagtgagt ttcaagttcg tcagtcagtg
     1861 caggaatttc ttcttgtttt ccctttttcc tgcgcgaacc aaagagtccc cacaaaacta
     1921 aaactacacc tatcaataaa agtcctagaa caaaagaaaa actattgttt cggacaaaga
     1981 gaaatagcaa gaccaatgct agaagccacg gccaatttcg ttttatcata ataaaccctc
     2041 catgtcattt atcgttgagt attgaattct tttcgttatt cgattacata cttattttag
     2101 catacggact actataaaga gtatcggact aaagaaggat tttctatatt tagagaaaat
     2161 ctaaggttta tctatggtaa actaataagg agtttttgag aaaaattgaa aaatgaaaaa
     2221 aaagcagtta tgattacaaa gaaagcagga gaagatatgc tgaacaatga gcaatttgaa
     2281 gaaaaaacaa tcagtcgcaa agaaatctat agcggaaaaa ttattgatgt tgctgtagac
     2341 gaagttcgtt tgccagatgg aggaacttcg aagagagaac tggtatttca tcctggcggt
     2401 gtcgggatca ttgcttttga cgaacaagat cgtctgcttc ttgtcaaaca atttcgaaaa
     2461 cctttagaaa aagtgatttt ggagattcca gctggaaaaa tcgatccagg agaaggacaa
     2521 aatcccgaaa tgacagcagc tagagaatta gaagaagaaa ctggctatcg tgcgaagtcg
     2581 ctgagccatc ttacttcgat gtatctttca ccaggttttg caaatgaagt ccttcatatt
     2641 tatcatgcac aaggggtaga gaaggtcgag aatcctttag cacaagatga agacgaagta
     2701 ctggagttgt atcatctaac tcttgaagag gcgcaacaag cgatgaaaga tcagttgatt
     2761 tgtgatgcaa agacgattta tgcgattcaa tactgggaac tattgaccaa aggaaagtag
     2821 agggatcggc ttatggccaa aggaccgctt attacgcgaa gcgaacttcg caagcgacaa
     2881 caagcacaag caagcgagtc attaaaaaaa cagcgaaaag cagaaacagc ttatcagcaa
     2941 gaagaaaaaa agattgccag cttttatcgt aaagaaagca aaaagaataa accaatcacg
     3001 aaaacaagga tcagcgaacg ggaaaagacg accaaatgga actctttttt gatgaaatct
     3061 cttattattg ttatcctaat gttatgtgtg gtttttttag caatcgcatt tatatagaaa
     3121 gaaggaatta aaaagatgaa aatcggtatt atcggcgcaa tggaagaaga agtaaaaatt
     3181 ttgagagaga acctaagcga accattatca tgggaacgcg caggcgcttt atttatttca
     3241 ggttcattag gtaaccacga agtgatcgtc gtacgttccg gtataggaaa agtcttagca
     3301 tctattacca caagtctgtt gatccaacaa tacggcgtga acatggtgat caatacaggt
     3361 tcagctggcg gaatcggtga aggcttacaa gtaggtgaca tcgttatatc agacaaagta
     3421 gcttattttg atgcagatgc aacaggattt ggctacaacc caggacaatt accaggaatg
     3481 cctctatatt atgaagcaag cacatatttg cgcagtgaaa tggttaaagc agcaaaagca
     3541 acgaatttga acgcaaaaga aggattgatc gttacaggtg atacatttgt cgactcgcca
     3601 gataaaatca aagaaatctt aacgaatttc cctgaagcat tagcttgcga aatggaagga
     3661 gccgctgttg gacaaacagc tcgtcagttc aatatcccat ttttgatcgt acgtgcaatg
     3721 agcgacacag cagatcactc ggcgacacaa agctttgacg aatttatcga agacgcaggc
     3781 aaacgatcag cagaaatggt gatcgaattt gtcaaacatc tcgtataagg aggactaaga
     3841 tgaacgcatt gatctcgatt gactacacat atgattttgt tgcagaggat ggaaagctga
     3901 ctactgggac agcaggacaa gtaattgaaa aaaagctgac tgctcataca aagaagttta
     3961 ttgatcaaaa ggattttgtc gtttttgcca ttgatgcgca tgatccaaaa gattcctttc
     4021 atccggagaa ccgcttattt cctccgcata atgtgatagg aacgggtgga cgggaattgt
     4081 acggctcttt aaaaagttta taccaagact atgagcaaga ggaaaatgtt tattggatag
     4141 acaaacggca ttattccgct tttagtggaa cagatctaga catccgtttg cgagaacgcc
     4201 agatcacaga tatttatttg accggcgtat gtacagatat ctgtgtgttg catacagcag
     4261 tcgatgcgta taatctagga tacaaactcc atattttcaa agatgccgtg gcaagtttcg
     4321 atcccgttgg acatgaatgg gcattgagac attttgagtc cacactaggc gcagaaattt
     4381 tataaaaaaa caaagtgatt tgataaaata aaaagcacaa aataataaaa gaagagcata
     4441 aaacaaacga ctttgtttta tgctcttttt tatacgttat tttaaaaaaa cttattcttt
     4501 ttacgttaat aaatctcttt attatcaaca acttaagtta tattttttct ttttataaaa
     4561 tacacttatt ataattttgt tttttattga cagtaaaaat aaattaggta atactttaag
     4621 taaacgcttt ctaatttgtt acgggaggaa tacacttgtt aaaaaaacgt ataaaataca
     4681 gtgcgctaat gatgaatctc cttatgctca gtaccccggt tttaatttct gtcgttgaag
     4741 ttgctgctaa cgaacaacag tctgtgaacg aagaaaatgc acacgtgaac attgaaggga
     4801 tgtcaacaat ggaggaaata ccaagaacta gtgaagatat ggatacggaa gaaggaaatg
     4861 atcctatgat gaaagaatca gaaaatgaaa agacaaaaaa gcaatctgaa gaagcaaatg
     4921 aagagtggaa aggggttgtt ttcggagaaa gcacaagtgc tagtaggtat gccattgaac
     4981 cattagaaga cggcgtgcgg ctctcttcca cgaataacgg cggaaaattc cagtcaagcg
     5041 gatcagatgg aatgacctat tattatcaag caatccccaa agacatcaac tttaggatgc
     5101 gggcgacgat cgaaatagaa tcttggacct atacgaatgc ccaagaagga tttgctttaa
     5161 tggtaagaga cgcagtacca aaaaatcatt tgtttgggca ggcattttat tcaaattcat
     5221 ttgccatact aggcagtcgg atcgaatatg tctgggactc ggatagacaa gaagtggtca
     5281 acacgagcgg cagcaaatat acgatgcgac ttggattggg gacacgggca atcacaggaa
     5341 tatcatctga agatccgcaa gctgcgccag ctcctgggag tgtctcagtg gaaacaacac
     5401 ctttagagac ttcagcaggg ttcttggaaa aagaaacagg ttcttataat atattcggaa
     5461 acggtcatgg gaatctgttt ccggccacga actcgattac taaactggac gtagagatga
     5521 aaaaaacgaa tactggttta gaggcatatt attacgatcc tgaaacagga gaagaaatgg
     5581 cttctgacat catgtatgac tgggaaaaat tatacgcttc agatgaatcg gttatttatg
     5641 caggatttgc tgcagcaagg aatatgacga tcaaggtaga aggaatcgaa gttgcttact
     5701 cagatcctgc gacagatcct cctgggcaag aaagacctac tcgcttgatt gatccgattt
     5761 acactgtgac ctctagtaat cattcaggaa accaagatca tactatgtcc tttagagcaa
     5821 atgctgacgg attattgaca attaaaaata aagcaacagg agaggtgctt aagaacgcaa
     5881 aaaacttggc tatcactgcg aatcgggaat ttgattatca gacaaaactg atggaaggaa
     5941 cgaacgaatt tactttcagt tttacgccag ataaaaacta tccgccagaa gaatacgtag
     6001 agatgactag ttatgaaaca aaagagattc ttcatacagt ggactaccgt aaacctaaat
     6061 attcgacagt ttatgtaaca ccagaagggt cagatgcagg aaacggaagc agacaaaatc
     6121 cattatctct gacacaagca ttgagatacg catatgctgg gcagacaatc gtcatggcgc
     6181 ctggaactta ttccttcgaa agaggattga cgattccaaa aggcgttaac gggacaagag
     6241 aaaacccgat tcagttgatt gcagagaaaa atccagaaaa caagcgtcct gtacttgatt
     6301 ttaaaggaac tggaaatggg atgacactgt tcagtcacta ctggggttta cgagggtttg
     6361 acattacaaa tagtgcagct atgcaaaaag gattacagat ttctggaaat cataatttga
     6421 tcgaagagat tcatgcttat cggaatggaa atacaggaat ccaaatcagc ggtagctcaa
     6481 atgatccttt cgaagcatgg cccgcgcata acttagtcaa gaactgtaca tcttttgaaa
     6541 atgccgatcc aggatttgaa gatgccgatg gatttgcggc caaactgaca ataggagaag
     6601 gaaatgtatt tgacggttgt atcgcgtatc ataatgcaga tgacggttgg gacctttttg
     6661 ctaaaaatga aacaggttcg atcggaaaag taatcatcaa aaactcagtt gcttatcgaa
     6721 acggctgggt accaggaatt gagggagaag gtaacggtaa tggattcaaa atgggcggtt
     6781 cttctttaac gggccctcat gaattgatca atagtttgtc tttcgaaaat cttgcgaaag
     6841 gaatcgattc caatagcgga acagatatta tcgtcaatag cagtacaagt tttaataacg
     6901 gttcatataa tgtggctctt tatacctctt cagcaggaaa cacagactac catgcttctg
     6961 gtatcttatc tttccgaaca gagaatcttg agatgagaga gcagatccgc ccattgaatc
     7021 aaaacgaaga tcaaatctac cactcgttga actactattg gaatgaacga gagaaacaat
     7081 cagaaaatac tcttggacat accatatcaa aagattggat cgagtctcta aatacatgtt
     7141 cgaaagaagg tcagcagcct gttacgcgct atgaaaatgg aagtatccat gttcatggac
     7201 tgatgcagat taaaccgggg aatcgtcaaa cagaaaagga aagagtggat ggtcttccat
     7261 tatctgtagg tgctattttt gacggagaaa cctcaccgtc ttcggaatat ccagagtatg
     7321 caatcgtaca agaaccaaat cagacaaaac caaatgaaca aaataatccg attattatac
     7381 aagaagcaaa tggtgtggcg gagaatgcag aaggaaatat cattgaactg aagcctttta
     7441 cagtttttgt gtcaactttc acaccaaatc aaggaacgcc tgtctttgaa gatatgcgtt
     7501 atttcccaga aaagatccaa atcgttgaaa cacagtcaag tgctcaattt tctaatcata
     7561 ccgctatttt ttcagcacct tcaaaaggag aatatacgct agaagtcact tatcgttcgg
     7621 aaatatttga tggggaaaaa tggtctgctc atacagaatc tgttacccag caaaaagcga
     7681 tcactgtgcg agaaactgcc gatgtgcccc cgggaaatga caaaccagaa agagaaaatc
     7741 caggaaatga atcaccaaat ggaaaaccaa atggaaaacc ttctcatccg gataggacag
     7801 caaaagaaag tgaaaaaaat ggcactcaag gactcccaaa aacgggagaa tttaataacc
     7861 cgttgctagc attagctgga ggaattttgt taatcggtgt agttgtttat gtcatgaaac
     7921 agcgaaaaaa ataagtggaa tttctccttt tatgatgtaa aacaattgac tgaggaaagt
     7981 aaaaatagaa ggaaatgaga ccaatagatg tctcacttcc ttctatcttt ttatagatga
     8041 ggttctcctt gctgcttttg attggctgtt cttattactt gatgcaggac agctttttca
     8101 caatctctcc actaaacggt gttcaatcag ctgaccctcg gtattagctc aacaaacggc
     8161 aaaatatgag gaactatttt tccgtttttt tctctaatac tcaggtcagg acgctgattt
     8221 cacaacctct cgataaacgg tgttcaaaag ccagtttctc ggtagtaagt caaatttatg
     8281 caaaaatgtg gaaagccatt ttcacaaaat ttttcttact acttgaaact aagcagcttt
     8341 ttcacaacct ctaatacatg gtattccaga agtaacttct tcgaaaaata agccgaaatg
     8401 tcacaaaaat ttgagaagcg attttcccaa attccttctt atttcttgaa gctgaccact
     8461 tctggcacaa cctcttacca gattcgcact cttttttctg gcgctagata catagcgtct
     8521 tgttccgtga taccaaatgt ttcatagaat tcgtcaaggt tttttggttg cacgtttgct
     8581 cggagtttag caggggcatg gacatcgatt tgcagtaaaa gttgttggta ttcttttttt
     8641 gcttttgtac gccaaatcgt tgcccaattg acaaagaatt cctcgataga aagatctgat
     8701 tccgtttttg ctgcttccaa tgcacagctt agaccgcctg catcagcaat attttccgaa
     8761 acggttaatt ttccgtttac ttttccgtca gcaaaaggca gaccatcgaa ttcttcgatc
     8821 attgcttgtg ctttgttttg aaattgtgta agatcttctt ctgtccacca gttgttcaag
     8881 ttgccatatt cgtcaaataa agcgccgtta ttatcaaatg catgggaaat ctcatgagcg
     8941 ataacagctc cgattccacc ataattagca gagcttgatt gttctagact gtaaaatggt
     9001 gcttgaagga tcgctgccgg aaagacgatc acattccgaa atggatgata ataagcattg
     9061 accgtgtcag cactcatttc ccattctgaa cgatccactg gtttgttcca tttgctgaaa
     9121 cgatctttta atgtgagttt gctgaaaaca agagtatttg aaagcaaagt accaccctga
     9181 tctgcaggta ctgttttgaa ctggtcgaat aatgctggga tcttatcggg gtagcctact
     9241 tggataccta agttatttaa ttttatgacg gctttttctt ttgttgcatc cccaagccag
     9301 tcattttctt ctagacgttt tttataaacg gcgatcattt ttttcaccat ttgttctacg
     9361 tcttttttag cagcttcgcc aaaatatttt tttccgtagt aatcgccaac tacttgatcg
     9421 aattgtccag aggcaaggta atacgctgct tttttctgag gcattgcttc gtctgttcca
     9481 gacaatacgc gagaatagat tccgctgact tggcggaatt cttctgatag ataaccacta
     9541 agagaacgta ctgttttaat gatcatccag cttttgaata acggaaaatg agcttctgtc
     9601 aggatgtcat tcaaatgatc gaagtacact ggatcagtta cgatcacttt atctggagtt
     9661 gtcccaatca aacccgtgac aagttttttc aagtccagtt tatctgtgta tttaataaat
     9721 gtatcaaaag attgcggatt atacattttg ctgtaatctg cgttttcttc tgcacttttt
     9781 acatgaggag caatcaattt atcaaattgc actgcttgtt ccgtgattgc ttctgcctga
     9841 cttttttctt tgccggcaag ctctaataac tgaacagcca tatcaaagaa aacgcctatt
     9901 agttgtgcac cgttgggatg gtctgatgca taatacgtct tatctggaag gaataaagaa
     9961 ggcggataag caaagaaggc gttcgtttgc gcattcttca tatctgcatc tacatccaga
    10021 ttaaatggca atggtaatgt atccaacagc cattctggaa attgcttatt caattcgtcg
    10081 aaagactgca atgattcaac tttttcgatt ataggttgaa gcggttgagc atgcaactca
    10141 tttcttcggt cataatcgtt tgccagttga taatatgcaa ggaaatgttt cattttttct
    10201 gaagacactt tttctggatg ggcaagcaga tcgtctgttt ctcccatcaa aagttggtcg
    10261 atcccgtcaa ctaaatcttg gaaaccgcct gtagctggtt tatctgcagg gatttttgct
    10321 gttttttgcc attcttcatt gactgcttca tagaaatctt gtttcaatcg ttctttgttc
    10381 attcatcgat cgctcctttt ttgtttcgtg tgcccattat cttacaaaat gaaggaatta
    10441 acaaatagaa cataaaaaat tgaaaattcg aacaatcaaa ctgatttgtt tttttcaatc
    10501 cttcacttat aatgaaaaaa aggaggcgat cccaactgaa tcaagtagtg gataaacctt
    10561 tatatttcaa agtag
//
LOCUS       DACDRN010000081        10348 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDRN010000081 DACDRN010000000
VERSION     DACDRN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.081"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            201..536
                     /locus_tag="IWJ80_002381"
                     /pseudo
     CDS             201..536
                     /locus_tag="IWJ80_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            701..928
                     /locus_tag="IWJ80_002382"
                     /pseudo
     CDS             701..928
                     /locus_tag="IWJ80_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1054..2025
                     /locus_tag="IWJ80_002383"
     CDS             1054..2025
                     /locus_tag="IWJ80_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9462074.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            2165..3478
                     /locus_tag="IWJ80_002384"
     CDS             2165..3478
                     /locus_tag="IWJ80_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9462075.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            3521..4387
                     /locus_tag="IWJ80_002385"
     CDS             3521..4387
                     /locus_tag="IWJ80_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9462076.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            4384..5232
                     /locus_tag="IWJ80_002386"
     CDS             4384..5232
                     /locus_tag="IWJ80_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9462077.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            5246..6718
                     /locus_tag="IWJ80_002387"
     CDS             5246..6718
                     /locus_tag="IWJ80_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP9462078.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            6735..7607
                     /locus_tag="IWJ80_002388"
     CDS             6735..7607
                     /locus_tag="IWJ80_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9462079.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(7843..9225)
                     /locus_tag="IWJ80_002389"
                     /pseudo
     CDS             complement(7843..9225)
                     /locus_tag="IWJ80_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9687..9968
                     /locus_tag="IWJ80_002390"
                     /pseudo
     CDS             9687..9968
                     /locus_tag="IWJ80_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10042..10348
                     /locus_tag="IWJ80_002391"
                     /pseudo
     CDS             10042..10348
                     /locus_tag="IWJ80_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
BASE COUNT         3197 a         1758 c         2122 g         3271 t
ORIGIN      
        1 tatatccaat ttttgccata aaaactcctt ttaaaaaatg atataattta catgtaaaat
       61 ttacttgtag ttttactgtt atcatagcat ttttaaaaat aaattttaag tctacttata
      121 atttactttg gaggttttga atgaatgaaa ttaagcgcaa taaaaattct tttggtgttt
      181 atttaggaga gcctagctcg gaacagttaa ataaaggtga agctcgtcat gctctatgtc
      241 gaaatatatt ttatggtaga aaaggcaaac tttatcaaac ttattacgac ggtatggagg
      301 aacagctcaa cgcattagga ttagtaacta atgctatcat atattggaat tcactatatt
      361 tagaaaaagt ctttgaacaa attaaaacgg aagggttcga ctgttccgaa gaagtgttac
      421 gcaagcaatt tccattaatt agtgatcaca ttaattttgt tggcaaatat agtttccgct
      481 atgaaaaaga attatcgaat ggaatactac gtccgttaaa tatagagaat tattgagaat
      541 gttcgtcttt tggttcctac cgtgcatttt cgcacggatg ttccagatac tatttttgaa
      601 tgtataaaat tgaaaacaca agaatactaa taaatcaacg attcttgtgt ttttaatttt
      661 tcactaccgt gcattttcgt ttctatgttc catgtacccc aatcttgaaa aattaattaa
      721 agacttagat aaagccattg acgacttagt cgtggtgcta ccggagtatc aatgtttaac
      781 cagtatccca ggtattggtc ctgtttaggc tgctggaata ctcgctgaaa ttggtccaat
      841 tgaacgtttt tctgatgaaa taaaattgac aaagtatgcc ggcctatatt ggcgacagag
      901 tcaatccggt aaatctgaat acgaaaattt ttttacgaaa agcttcacgt ctaatatcta
      961 ttttaaaata aaaatattcc tgctaatttg gaatcgtttc cttcttttgg tataataata
     1021 cctgaaattc ttttgagagg agttgttcgt gaagtgaaaa ctactatgaa ggatgttgct
     1081 gcgttagcag gagttggtgt gggtacggta tctagagtaa taaatggagt aaaggtgaaa
     1141 gaggttacca aagaaaaagt aatggctgca attagtgagc ttaattatga gcctgatgaa
     1201 tatgccagag gtatgaaaac gaatagtagc aatacaatag ctcttatttt acctacgatt
     1261 tggcatccct tcttttccga gtttgcctac tatgtagagg aagctctcag ccaaaaaaat
     1321 tataagcttt ttttgtgtaa tgcgaacggc agtgcggaaa aagaaaaaga atatatccaa
     1381 atggtcaagc agagtaaagt agacggaatt ataggtatta cctattcaga cattgataaa
     1441 tatgtatcat caaatttacc atttgtcagt atagaccgcc atttttcgga acaggtttct
     1501 tatgtaacgg cagataatta tttaggtggg caaattgccg ctcaagaact acttactcgt
     1561 aactgtaaaa acttgtgcta tgttgggggt gtttcacctt accctaacga aacaaacaat
     1621 cgtaaagctg gtttttacaa tatttgtgag gaaaataaaa tagctgtgaa ggtattggat
     1681 atgcctgaac caattagcaa tttagaggaa caattgctcg cttttttttc ggataatcct
     1741 gaaattgata gcatttttac catcaatgat ttcatggctt tggatgtgat aaaagcccaa
     1801 aaaaaactag ggaaggaagc acctaaggat tttcaaatta ttggttttga tggagtcaag
     1861 ctatcgttgc aaaaagacta tttactatca acgattgtac agccgataga ggaaatggcc
     1921 aaggtttcag tagaaacact catggcgatg attttaaatg aagaaccgcc taagcgtgtg
     1981 gtagtaccgg ttttctttca cgctggagag acgactaaag agtaaaaaac cggtttataa
     2041 cgattttttt gagataggtt gacagaaagt gatttcaatg atatcttaaa accaattaat
     2101 tggaaacgtt accaattaat tgaaaaataa aacggaaacg tttcaaaaaa taggaggaac
     2161 atcgatgaaa aagagaatgt tagcaattgc agcaatttta gcattaacag ctggaggact
     2221 tgcaggatgt ggcagtaagg agaaaagttc aggtagcagt gatccaggag aaattaagat
     2281 ttgggttcag ttttctgatg aaacgcctga aggaaaagct tgggcagaga ttgtgaacaa
     2341 ttttaatgat gaatacgagg gtgaatataa agctgttact gagtatattc ctcgtagcgg
     2401 aagcggcggt ggatatgagg ataaagtaaa tgctgcaatt acaaccaata cccttcctga
     2461 tgttattacg ttagatggac cgaatactgc agcttatgct cattcaggag tcattgctcc
     2521 attagatgat tatttgaaag atgcggacat ggatgatgta ttagacagca ttattcagca
     2581 aggaacctac gatggcaaaa tgtatgcgtt tggtttctca gagtccagtg tgggtgtcta
     2641 ttacaataag tcgctgttca aagatgctgg aattacagaa gatcaattgc cgacgttaga
     2701 agatccttgg acttggacgg aatttcaagc aatttgccaa aagctgactg ataaatacaa
     2761 aacgcctgca attgatatgc aaatggcgtc gggagacgaa atgctgacct acgcatatac
     2821 accatttata tggtcgaatg gcggcgatgt cgttaatgct gatggaacca aagccgaaga
     2881 ctattttaac ggtaaaaaat ccgttgaagc gttggaattt attcaagaat tggttgaaaa
     2941 aggttacacc acaatcagtc ctattgaaaa aggatttgaa actggcaaat atgccatgat
     3001 gctgagcggt tcttggacaa tggcagattt agaggaaaac tatcaagaga ttgattatgg
     3061 tattttgcct taccccgttt cagatgcaac tggggagtta gtttcaccaa ccggaagctg
     3121 gcagttagca atgactgaga aaacagagaa aaaggatgct tctgcggcct ttattaagtt
     3181 tgcaaccagt acagaatcta gtcgtattat gagtttaggg aacagtgttc tacctatcag
     3241 aaattcaaca gttgatttaa ttaaagatga agtgtcggaa ggaatgcagg tattaattaa
     3301 gcaaaaccaa ctttctggac atgcccgccc agtggttgta gcttatccgc aagtatctag
     3361 agcattccaa caagcagttc aagatattag ctacttcgag gaaaacccag atgtacaaaa
     3421 agtagcagat agtcgcgctg ctgaaatgca acaggcaatt gatacttcac taaaatagaa
     3481 gtttctaaag ggggccaatc aagtgttaaa aaaaccgaca atgaaagaca atttgatagg
     3541 ttacggcttt ctagcgccag ctttgctgct gctgacgatt tttctgatca tacccgtaat
     3601 catggtaatt tattatgcct ttactgatta ttatttgtta acgccagatg ctagacaatt
     3661 tatcggctta gaaaatttta agcgactgtt cgctgatcca atttttgtaa aaagtatttg
     3721 gaacacggct aaatttgttg tttggattat tccggtccaa ataggggcag ctttaggaat
     3781 ggcacttatt atcaacaaaa atcgtaaagg aaatatcttt tttaaagtag ccttttttgc
     3841 gccagtcgtg atgtccttag tcgttatttc gattttatgg ttgtacttgt taaatcctaa
     3901 tgaaggtctg gttaatacgc tatttgcaaa gattggaatt gcttcgcagc cttttttgac
     3961 tagtcccaaa caagcaatgt atacgcttgt tttcgtatcg gcatggcaag gtgcaggata
     4021 tcagatgctg ctgtttttag ggggaatgca aaatattccg caagacgttt atgaggcagc
     4081 cgaaattgat ggtttttcta agtttcagca atttcgctat atcaccatgc cattattaaa
     4141 gcccacggcg atttttattt tacttaccac attgatttcg gcctttaaac taatcattca
     4201 gccaatggta atgactcaag gtggaccaat gaattctact atgacaatgg tttattacat
     4261 ttaccaaact ggatttacag accgtatggt aggctactcc agctccattg cgttaatctt
     4321 taccactatg attggtttaa ttacaattgc tcaaagaaaa ttggtaaagg aggatgatcc
     4381 agcatgacca agaaatttgg accgctaatg atcgtagaat attgtttgct gttcctttta
     4441 gcggcattat tcattttccc gatgctgtgg atggtagttt cctctatgaa gcctgaagca
     4501 gatgtctatc aaaatttagc ttccttcaaa gcttttttac ctagtttgaa tccagctaat
     4561 tggtttaaaa cttatcaaga ggttatttcc cggttcagtg tgggaactta tttattaaac
     4621 agtatttttt atggtttaac ctttgcctgt gggtcaatca ttgtaaactc tttggcgggt
     4681 tttgcctttg cgaaggttaa tttttcaggc aagaaaatta tctttggagc tttgttagct
     4741 ctgctgattg tcccgatgga gacggtttta attccgcaat ttaccatcgt taattcacta
     4801 ggcttagtga atactcgctt ggcagtgatt ttaccagcga tggccagcgc gtttaatatc
     4861 tatctgttta gaaatttctt tatcgcgatt ccagaagaaa tcattgaatc agcgaaatta
     4921 gatggtgcaa atattgttaa aattttcttt cacatcatgc tgccaatgtc taaaccagcc
     4981 atagccacag taggtgtctt atcctttatc tccagctgga atgattatat ttggccgtta
     5041 atggtcttaa ctgacagcag caaattttca atgcaagtgg caattactac gatcaatact
     5101 acacagccag tttatatcaa tcaagtgatg gctgttttaa ccattgcaac gattccactg
     5161 attattgttt atatcgttgc acaaaaatat attctgcagg gacttggcgg ttctggaacg
     5221 ggtataaaat aggagtgaat agtgaatgtt gcaaaaactt ttaaaggcca atgagtttat
     5281 tgagaaaaat gctcaaacag tcaatcaaga ataccggaat aaatttcatt tgatggctcc
     5341 gattggctgg atgaatgatc caaatggttt tgtttattat caagaggagt atcacttgtt
     5401 ttatcaatat tatccttacg acagcatctg gggaccaatg cattggggcc atgctaaaag
     5461 caaggatcta attcactggg agcatttgcc agtagcactg gcaccaggtg aaagctatga
     5521 caaggaaggc tgtttttccg gcagtgccat agaaaaggat ggcaagctgt atttgattta
     5581 tacaggacac aatgttgtgg aagggcaagt tcgacaagtt cagtgtttgg cagtttcaga
     5641 agatggcatt cactttgaaa aatatgccgg aaatccaatc atcgcagaag atcacttagc
     5701 ttctgttgca acgaccgaag atttccgcga tcccaaagtg tttcagcgaa aagaagacta
     5761 ttttactgta gtcgctacta aaactgcaga tgctcgtgga caaattgtga tgttccattc
     5821 caaagattta ctaacttggg agttttattc cgtattgctg gagggaactc cagaacaagg
     5881 cattgtatgg gaatgcccag acttgtttga actagatggc aaagaagtct tgattctttc
     5941 tcctattcaa atgactccac aaggcaatgc ttaccaaaat acgaactcaa ctgttgcttt
     6001 catcggtact gtcgattggc aaacaggcaa atttctagta gagaattttc atgaaatcga
     6061 tggcggttta gatttttatg caccccagac ctgtgaaaac gaagcaggcc aaagaataat
     6121 gattgcttgg atgcaaatgt ggcagcgtaa aatacctacg aatgatctgg gccatggttg
     6181 gtcaggttcc atgaccttgc ctagagagct gagggtggct gataatcaat tgttgcaacg
     6241 gccagtttca tcaatttatc aagtaattga tccgcaggaa aggattgaat gggcgaagac
     6301 tgaagagaat cgcttcttac tgaaggactt agtatatgac cagcactatt tacgcttgga
     6361 aatcaattta aaacaggcaa cgaaactaga acttcactat gcgaagggac ccgaccatac
     6421 attactgctt aactatgatg ttgaacggga gtgctttact gcttccaggg aacacatgcc
     6481 gtatgattta tcaggagctg aaaaggaagt gttgaaaaag cgcagtgtga cgattccttt
     6541 ggaaaataat tgtctaacca tcgaaatttt ccgtgatact tcagctattg agctttttct
     6601 aaatgatcgt ctgactatga gtacaacttt ttatgagaca gaaaaaggac aggattttct
     6661 tttgctggca gacagtgaag taaccttttc attggaaagc ggcttaatta atctatagaa
     6721 gggagagctc agtgatgatt tatggttcaa tcgaagcagg cggcacaaaa tttgtgtgtg
     6781 ccattagcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca acgacacctg
     6841 aggaaacaat acctttagtc attgacttct tcaaacaata ccaagcagat ttagcaggga
     6901 ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact tatgggtaca
     6961 ttacctcgac cccgaaagtg gcatggcaaa attttgattt catcggtacc atgaaaaaag
     7021 aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat ggtgagtatg
     7081 tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt acgggtgttg
     7141 gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca gaaatggggc
     7201 atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca tttcatggca
     7261 actgtttaga agggatggcc gctggacccg ccatcgaaaa acgcttaggg aaaaaaggac
     7321 aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt gctcaatgtg
     7381 cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt ggtgtgatga
     7441 aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc aatgggtatg
     7501 tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac aacgctggaa
     7561 cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg aaaaaaggct
     7621 cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat aaccagttag
     7681 gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat gagataagtt
     7741 cttgctcgaa agttatggaa atttcgatag ccgtaggcta cttgtattgt gcaatagttt
     7801 tttacaaaaa aaagggggtg cggatatttt ttttggacct ttttaacaaa tatatctttg
     7861 cttcttccga tattgaagag ggcttagcct tcctaaagat aacttaattc gttgattatt
     7921 ataccaatga atataatcat ccaatttgtt tataaactga ttgattgttg tattcttcca
     7981 gtcacgttga taaaacatct cgttttttaa tcgtccaaag aaaccttcgc acgctgaatt
     8041 atctggtgaa taactctttt tagacataga gtgaagcaag ttggctttgt tccaatagtc
     8101 caactaacaa tagcaccatc gtaacaatcg acaagtggtg ataagtagat tttccctgcg
     8161 agaattctaa attcagtaat atctgttaac catttttcat ttggcttctt tgctttaaaa
     8221 ttccgattta ataaattggg gtgtgcagga gaaatttccc cagcatacga agaatacttt
     8281 tttcgtctag aaaatttaac aaatagattt tcctcagtca tcaatctacg tacaactttt
     8341 tcagaaagct ttttacccat attttttaaa gcttggtgaa ttctacggta accatacgca
     8401 cagaagttat gattgaaaat cgtgataatt aatttgacgt tctttgtaat acttatctgg
     8461 tttctcaagt gcttgttttt gataaaaata gctgctttta ggaaactgga gctgttgaag
     8521 aagctctttc aaggtgaact gatttcgtaa ggcatcaatc aatatcgttt tttcttgatt
     8581 tgatagacaa agaagattga cgcctttttc tttttttagc agttcagccg ctccttcgag
     8641 taatgctttt tcgagctgta attggtgtac ttgtttttgt aagtcttcta cttcctttaa
     8701 aagtagctct ttttgttttt cctgattttt taaagtcata cacaatggaa aatcctttcc
     8761 aagaaccgtg tttttccatt gatatagagt aactctacta attcctccag attcggctaa
     8821 atttttccta ttagtttgcg gtttatagaa ttttttcaac acagcttctt tttgatcttg
     8881 tgttaacttt accgcacttt ttcggagttt tctagctcct ggtgcaagtt cttcacaccg
     8941 ctgccgcaag gactctctat taggataacc aagcatgcga atggttcgtg cataacattg
     9001 accatattca aaataatgat tcaccgctat tcgtttttgt tcttcagaat actttgattt
     9061 ttttcggtgc tcctgatgga gctctccgga ttccaaatac tctttatacc atttccgtag
     9121 tgcaccaatc gacggatacc ctaattctcg aatggttgca gcataagagc aatcgtattt
     9181 taaaaataac tgaattgccc ttacacgctc tttgtgggaa aacacacgga cattcctcct
     9241 aagtttattt tttcgtccag gattatgtcc gcaccccctt gctttctgat gacttattct
     9301 ggttctttga gtgtcaatgc atagatgtga tgtagctagt tagaaaaaaa ttttgaatac
     9361 tctttttttc tttcagactc ctttttaagt aagattttga agtgtgagtt acggggtcag
     9421 aaacttaaaa gtttttgatc ctttattttc aagtctgatt tggcagcttt tcggcagctc
     9481 atttatttat ggtcagaaat agatgctgaa attttaatct attagcaaat agtccagaac
     9541 gtatgaagag gagtcggata tcatttgtca ttaacaccat aaatatttat aaaattcgtg
     9601 ttcacctaca attaaattta atatataata ttcgttgtaa gggcttataa ttttttgtta
     9661 atgaacaaga taggaagaaa catttaatgg acagttttca gagattgtca ctagtaaatc
     9721 aatttgagtt attggctaaa tttgaggacc aaaacagatc ttattatgaa agaaaaatcg
     9781 aaatattgag agaaggttac gagtatcatt atgatgatga aatttggagt gatctttctg
     9841 agccatttcc gaaagaagat tctcgatttg tattagatgt attgaacatg tatcgagata
     9901 tcaattttag taaatcaaag ctaaacttag atgaaaacca aattactaat tcatactata
     9961 ctcatttcgg ttctgttgca aagttttcca aaaaatctat tttagtgtaa aattgagaaa
    10021 aaagacagag aggacagagt aatgaatcat tttaaaggca aacaattcaa aaaagacgtc
    10081 attattgtcg ctgttggtta ctacctgcgt tacaatctaa gctatcgtga agttcaggaa
    10141 ttgtgatatg atcgtggaat aaatgtttgt catactacga tttatcgttg ggtacaagag
    10201 tacagcaaag tcctctatta tctttggaag aagaaaaata gacaatcctt ctattcatgg
    10261 aaaatggacg aaacctatat caaaattaag ggacgttggc attatcttta tcgtgcaatt
    10321 gatgcggacg gcttaacctt agatatct
//
LOCUS       DACDRN010000082        10261 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDRN010000082 DACDRN010000000
VERSION     DACDRN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.082"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..497
                     /locus_tag="IWJ80_002392"
     CDS             <1..497
                     /locus_tag="IWJ80_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HAP9462080.1"
                     /translation="ATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFT
                     NGFSIFNYNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTK
                     RIVGVQLCSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGL
                     NIET"
     gene            609..2060
                     /locus_tag="IWJ80_002393"
     CDS             609..2060
                     /locus_tag="IWJ80_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9462081.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            2409..3080
                     /locus_tag="IWJ80_002394"
     CDS             2409..3080
                     /locus_tag="IWJ80_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP9462082.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            3628..5067
                     /locus_tag="IWJ80_002395"
                     /pseudo
     CDS             3628..5067
                     /locus_tag="IWJ80_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            5412..5579
                     /locus_tag="IWJ80_002396"
     CDS             5412..5579
                     /locus_tag="IWJ80_002396"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9462083.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            5838..6053
                     /locus_tag="IWJ80_002397"
     CDS             5838..6053
                     /locus_tag="IWJ80_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462084.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(6241..6528)
                     /locus_tag="IWJ80_002398"
                     /pseudo
     CDS             complement(6241..6528)
                     /locus_tag="IWJ80_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(6715..7626)
                     /locus_tag="IWJ80_002399"
     CDS             complement(6715..7626)
                     /locus_tag="IWJ80_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9462085.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            complement(7645..8649)
                     /locus_tag="IWJ80_002400"
     CDS             complement(7645..8649)
                     /locus_tag="IWJ80_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9462086.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            complement(8646..>10261)
                     /locus_tag="IWJ80_002401"
     CDS             complement(8646..>10261)
                     /locus_tag="IWJ80_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9462087.1"
                     /translation="ETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAA
                     LSLGTEIVVPNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKIL
                     SVSPDENKGKDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLT
                     GIMLFSQILFIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAF
                     SISTMFFNISATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKP
                     QMLMNRTLRQLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHE
                     IPSDLNKEKPIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYN
                     FHKGIEKTKKAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKR
                     RENVPIKAKVQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVK
                     RRSTLSKRTNRKVQKSLNPHPKPRSGEKK"
BASE COUNT         3214 a         1819 c         1914 g         3314 t
ORIGIN      
        1 gtgctacaac tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg
       61 ctctaagaac agcttatatt gcaactcaac atattgacaa tcaagatttt acaccttctg
      121 gttcccaatt tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga
      181 atttaaaaac agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca
      241 ttagacctgt ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa
      301 atacaaagag aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat
      361 caatgttttc tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt
      421 atttatttca tcctagtttt agccaaccct ataattttat tttaaaggca gtaacaggat
      481 taaacattga aacataatcg ttactaaatg aaatcaagaa atatactgta ttactcaatt
      541 cattgaaaat atttatcata atttttaata gaaaacacta ttaaagagaa aaaagagagg
      601 taaatcaaat gaaaaaatct ttaaaacttg catcagcagt tatattaatg tcccaactct
      661 ttatagcaac acctttccat gtgttggcaa gcgaaaattc aacatacaaa attgcaaaag
      721 aaagaaccga gacgactgaa atgagtaatt ccgatgtaac agcagagagt agtgaacttt
      781 tgacaacttt atcaagtgaa agtagtacga cttcaaatgc tgagtcttca acaacttcat
      841 caagtgaaag tagtacgact tcaaatgccg agtcttcaac aacttcgtca agtgaaagta
      901 gtacgacttc aaatgccgag tcttcaacaa cttcgtcaag tgaaagtagt acgatttcaa
      961 gtgccgaatc ttcaacaact tcaagtagta caagtgaaat aacttcttcg aaagtgacaa
     1021 gaccaaacca tatagagtct tcaaaaaagg aagcgccaaa aacggaagga caaacaaaaa
     1081 atttttcgac accacagcaa atagaatcaa cagaatcttt ccaatcacaa ccaatactat
     1141 ctggtaatga agaaattcat tttgacaaaa atgaatctgt agaaaatttt attatgaaaa
     1201 ttggtgagtc tgcgagaaaa attggtcaga aatatgattt atatgcttct gttatgattg
     1261 cacaagcgat cttagaatct gccagtggtc aaagccagtt agctcaggca ccgaattaca
     1321 atttatttgg gataaaagga acttataatg gaaactttgt cattatggta acaaacgaag
     1381 atttgggtaa tggaacgctt tatacgaccc aatcaaaatt tagagtgtat gaaaactatg
     1441 aggaaagttt tgaagattat gccaaattat taactaaggg aattagtgga aataaagatt
     1501 tttatgcagg agcattaaaa gctaatagta aaacatatcg agaagcaact aaattcctta
     1561 caggacggta tgcaacagat acacaatact atttgaagct aaatgaatta attaaaacct
     1621 atgatttaac caactatgat aaagaagtta aaggtccaca aatgagtaag actggatacc
     1681 aagtgcctat tcataattat tcaataacca gtttgtttgg aaatcgtgga aatgaatttc
     1741 accgaggact tgatatggca gctacacagg gtgagccgat tcatgcagtt aaagctggta
     1801 aggttgttaa agcagagttt cactactctt ggggaaatta cgtggtgata caacacgaag
     1861 atggatcaac cgcgttatat gctcaccagc aacaatacct agtaaaaact ggagataatg
     1921 ttttgcaagg acaaataatt ggctatgtag gaagtactgg gaatagtact ggcccacacc
     1981 ttcatttgga aatttgcaaa gatagtagtc tttctcagag catgttagtt gatccgaaaa
     2041 ccttattatt cggagactaa aaaaggtaat caatctgaca aagtaacaag aaaatggttg
     2101 gattcttaga cctttgatca ggatattgag actggaattg atatctcgat tacgatgagt
     2161 ttgacaaata aaacaagtcc attatcgaat atcgagtgta ttattgccgt ctttatgctc
     2221 atattttgag caggttttac tagatgaaaa caatttatct atcttgagga tttcgtgtac
     2281 gtatcgttca gtcttgtatt ataaattagt cataaaatca gactaagaaa aattgaaaat
     2341 atttttttct gatttattaa aagttaattg aaattatatg gtacttcttt cttgggaggg
     2401 taggtgcagt gaaggaaaga tatggtcacc ttactgttgt tgaggagatt actcataatg
     2461 gatataaaaa atatcgttac ctatgtgact gtgggaatga aacgattgta tatcgatcaa
     2521 acttactatc aggtagaacc aaaagttgtg gatgtggaaa ttataaaaat aggcataaat
     2581 ataaaaattt gacaaataaa acttttggta agttagttgc aatcaagcca actaaactgc
     2641 gaagtgaagg aactgttgtt tgggaatgtc aatgtctgtg tgggagaaaa aagttagtat
     2701 cggcaaagcg acttctaaaa ggtgatgtaa aaagctgtgg atgtttgaaa tcaaaaaagc
     2761 atcacattga aaataaaaaa ttcggattac ttactgcaaa gtatcctttg gacaataact
     2821 gttcatggaa aacaaaatgg gtttgtcaat gtgagtgtgg cagtatgtgt gagataagtt
     2881 atagtaactt attttttgga catactcaaa gttgtggatg tttaaaaagg caagaaacaa
     2941 ggacatgtgt tgaagggaca gtaatagagt gtctaacgtc aaaattaccg aaaaataata
     3001 caagtggtgt taagggagtt tatcgttcta aagagaaatg gattgcgtat ataactttaa
     3061 aaaaaagagg tactatttag gatcgtatga ttctattaag agtgcagctc aagttagaaa
     3121 agatgctgaa aataaattgt ttcaaccttt gattgacaaa tatcgaggta atcattaatt
     3181 attgtggata ataattaaca gtgatatata aacatctata atatattatt catactattg
     3241 acatttagta taatcctatg gaattgaata tcatagcaat gtatttgaaa agcaaattgg
     3301 aaaacaaata ggtgcaacac cataaatatg aaaaaattgg gaaaaagtag aatggaaatt
     3361 ttcttacgtc tcacgagata gctcacacta ttaatctgaa gaacaatgaa gtcgactttt
     3421 agaaattgct agaagaaagt aaagcgaatt atcactagtt actatagagt aagttcaaat
     3481 aaagcatctc aaacttacat ggttacatga gaataacaaa tcgaaattac tcaagcttcc
     3541 tatggctaga gtcgcgagaa gttgttgggc agtagacata ggttatattg ttcttagcta
     3601 cacgatcatt agaaaggaaa tcctattatg catagttatc aagaagatgc aaaagtccaa
     3661 aaaaatcgct ggtggatttt gattgctata tctatgttta ccttcatgtc cacattggat
     3721 ggcagtattg tcaatattgc actacctacc atctcagatg atatgggcat accaatgaat
     3781 caagcggaat gggtagtgtc cgtttatctg atgattgtct gttcttgttt gttgctgttt
     3841 ggtaaaattg gcgatagctg tgggaaaatc aaagtatttc ggattggtac gctgatcttt
     3901 gtcttgggct cgctgttgtg tgggttcaat cattcgctag gtatttaatt gttcgcacgg
     3961 gtggtacaag ccattggtgc tagtatgact atggctacaa atgccggaat catcacggaa
     4021 gtctttccca tacatgaacg tggtcgggca ttgggagcaa tcggtgcgct tgtttcatta
     4081 ggctcgattg ctggaccagg tcttggcggc ttgattttgt cccaattttc ttgggcgtac
     4141 attttttgga tcaatgtacc ggtgggaatc gtaacgatgt tgattgctga aaaattctta
     4201 ccaaaagata tcaccatgag cggccataca atcgatggag ggggctttgc tgccattgcg
     4261 gctacgatta tcagcctttt tggaacaatc tttttggggc aagaaagtgg ctttttccat
     4321 ccagtccccc ttgtactgtt cgtaatttca gtgattgcct ttgtcagctt tttaaaagtt
     4381 gaaaagaaag cagcctctcc attgatttct ttcggtattt ttaagaacaa gatttttacc
     4441 atgagcttga ttacagcctt gttgattttt tcttccaact tctttgttaa tgtagtgatt
     4501 cctttttatc tgcaaaatgc tcgcgggttg agtcctagct atgccggact gttgatgatg
     4561 gtctttccct tgatgatggt cgtcggggcg cctttaagcg gatacctgac cgaccgggtt
     4621 ggcccggaga tttttgtatt agtaggcttg ggtttattgt cggtaaccca agtcatgtat
     4681 atgtttttgt cagtggactc tcctttgtgg ctgtacattg tcgctactgc catcatgggt
     4741 gccggcaatg ccttgttcca gtccccaaat aatacgatgg ttatgagttc tgtcactatc
     4801 gaaaatttgg gactggcagg ctccatgaat tcctttgccc gaaatttggg gatggtcatc
     4861 gggatttcgc tttctacgac ggtcttatat caagcgatgt ctgctaagat gggccaacga
     4921 gtaactaact atatcgcaga tcggccggat gtctttttat atggaatgaa gattaccttt
     4981 ttaggcagct ttgttttgtg ttttgttgcc tttttcctga ctatctggcg tatcaaaaaa
     5041 caaggaggta ttcagaaaaa tgtctgaaaa accattgttg aagaattttt ggaggcctgc
     5101 gtatgggtga gtgggatttc catggatgat ggaagatgaa gtgaacttct gaaaaccgaa
     5161 aaactcaagt attatcttca aaatactagt actcaattag atgctcgatt tgattacaag
     5221 aagactgcga aagccactgg caaccgcaag ctaggtatcc accgatggac tacgttttag
     5281 aggcaactgg ttatgaatac ggagctaacg ctctcatctg cataagaatg caacacccag
     5341 attatgtaaa gattttcgat acttctgtca aaaaaatcat caagatagat aaatgatttc
     5401 cgtctagtca aattggttca gaatgtggac acacagatgg taagaagtca ttcacaattc
     5461 gagaatggac ttgtcctatt tgccgtacac atcatgatcg aaacgtcaac gttagtatca
     5521 atattttgat caagggctat gtatacaagc attgccttag aaaacatcaa gaaccttagg
     5581 aacaacgggg atagattggt taataagaga tatcgctgtt agtaaagaca taggcttgca
     5641 agtatactct tttatcaaga attccgtggc tttagtcatg ataggtttaa tactatgaaa
     5701 attttcttta gcatttttga tttaggttcc ttttctaatt ataataaaat gaccactaca
     5761 gtaacctact gaaatatata aacagccact gtagtaccat tttcaaagta gtctatgaga
     5821 ggagaatctt cattcttatg aacgctggaa ttgcggatat gaatttaata aaaaaaaccc
     5881 ttaatgattt tacttccaat tcaatatcaa aaggtactgg tatcaattta agcacaataa
     5941 aaaagctgaa aagtggtgaa agatcagtgg aaaagctgaa tcttttagat gctataaaaa
     6001 taacggaatt tgctatgaaa aatggaaaag ctgagattga aatatggaga taattatcgt
     6061 ctagtgaact acttgccact tagctgaatt taacgatcct tagtgcttga agtgggagat
     6121 tcttgggaat agagcatact tgcaagctta tgtctttatt aacagcgata tctcttatta
     6181 accaagctat ccccgtagtt ctaaggtttt tacttttatc taagctaatg atcgtattca
     6241 aaggaaattt ttgaccctta gaaagtctca ttttatcaga catgagctta ggcattgctg
     6301 aattttgtta attcggtttt aggttgtgct ttgagcataa tatgaaagta gtctttatca
     6361 cgatttcact caactaaggt aatgtgatgt gattctgata ttctttcaaa gatagttttt
     6421 tgcataagct aacattacat catcaatgag atgtttacaa tgtttcacta ctaaaataag
     6481 gtggtaacaa agaagaaata atgaatgatt atttgtatct aattccatgg tatatcaacc
     6541 atttcttata tagactgatt atgacatgga ttaaaataaa aaggcactat gtcttaattg
     6601 tcggttttcg agttaccgaa cggcattcat cacccaccta tagaggatgg gcgacttctg
     6661 cctgaaataa gttaaactac ttataacttc tcataaattt agattgagtc attattattt
     6721 aacatcaatg attttccagt ttccattttt ttgtaatatt aatttgtact gaaagtagct
     6781 agtggttttt gatacattat caaggtattt tatctttgta ctcactagaa ttttttttcc
     6841 gttttgtagg taaactggat cctctaactt cgaaaacttt aagtcaatat tgattggtcg
     6901 catcgcatca ttttcaacat aatactccaa ttctttttca gaagctgatg gatataattt
     6961 aaagaacgtt tccaaaaact cagcgatttc ttctgtagtt ttggcatcaa ttttcgaatc
     7021 attttctact tgttttgtct tgtattttgc cctatcaggt tttgcaatca tagttggcaa
     7081 actcgttact atataattcc tgttttcatc ttcaaagatc gtcactcggt atgcagaaga
     7141 aatcgattgt gccttttttc cctctgtaat cttttgctcc actaaaaatg aaacgacaaa
     7201 ttcctccgag ctcttttcaa catctaagac ttttactgac tgaacttcgg aattagtggg
     7261 aatatctgct cgaaccatat cttgacttaa agcgaaacct tcttctgtaa ggtactgttc
     7321 gaggttgttc attctctttt caattgcttc tttgttattt ttccacgaaa aatattcttt
     7381 cgaaaaatcc ttcgtaaagt tttcaatacc agaagtattc actactttct ctttaataac
     7441 cactttttca tgagttgtat gttgatcaat ggcggtgaaa tttttataaa cgccaaaagt
     7501 aagactacaa gataataaaa gccaaagggc aagcaccatt tttcgatgct tacccacgga
     7561 tacgactcgt tcttttttct tttttaggct cttttccttt tgattccgtt caatttttat
     7621 cttcattctt tttcctcttt tctcttattt gtaccgtcca gcaccgataa gatgctgctg
     7681 ccaatagctt tctgttagat tcgtccagcc tactggatct cctgcatgaa acatgcgatt
     7741 ctcccctgca taaataccga catgggtcac ataagtaccg gcatcataag tcgaatgaaa
     7801 gaaaaccaaa tcccctggtt tggcatcttt taggtcaata tgggtcatgg catcatactg
     7861 atcttgagcc acccgaggaa gtttcagacc aactttggca taacaccatt gagtcaagcc
     7921 actacaatca aatcctgttt cgggtcttga cccaccaaat acataagccg ttccttcata
     7981 cttaaacgcc tcgtcaaaaa tcccttggac tgttttatcg tcaaactgtg ttgtagttag
     8041 gtattgcttc accaataagc aataaaacat attgccatag ttatagcgcc atccaccatt
     8101 ttccttattc gcgatgggat tagggtaatc gattttttga ccgcaagatt tttcctttga
     8161 aaaagattca gctaaagcaa aactatgctt cttcccgttt tttgccacat agtccaaata
     8221 accaccgcca aagttatagg cctgaatgat actatcttga tcacaaccta attctttgga
     8281 acgcttaacc aaagacgcaa aatagagaca tccttgtttg atggatgcct cggtacttaa
     8341 tgaattcggt ggtaaaccgg ctgactcaga actttgcatc acatcttcca ttgtgccacc
     8401 agattccact tgaataatcg ctaaaagaat cggaacatac tctgttatat ccgattcttt
     8461 ggcatatttt tccaccattg cacggtgttt caaaacctct tctgagacac tactcccccc
     8521 aggaggagca cctgaactac tacttccttc actgtcttca ccaaacaata agcccacaca
     8581 catcaagata cccataaaag agattgttag gaaagttaac gaaatggcac ttaatttctt
     8641 tacgttcatt ttttctcacc tgaccttggt ttgggatgag ggttcaaact tttttgtact
     8701 tttcgattgg ttcgtttgga cagagtagac cgccgtttca cttgttgaaa agaatttttc
     8761 ttacttgagg attggtttat tccttttgaa atgatttctt ggctagcgaa ttttcgtttg
     8821 atttcgggat tcgaatttgc tctcacaatc cgctttggtg ctctatcctt ttgagattgg
     8881 acctttgctt ttatcggaac attttccctt cttttcccat gaggattccc tatttcgttc
     8941 aatactttgc gcttttcagc tattttttcg tctctacgtt gttgttgttt ttctcgcaac
     9001 tctcctcgat ttgctttttc ttggaccagt cctcgcttaa actcttccgg tgcttttttc
     9061 gttttttcaa ttcctttatg gaagttgtat ttcaagttgg ttggcgtatt ccgtactcca
     9121 tcctttactt gtttggtctt atctttcacc aaagcttggg tatccagaac cttgccgact
     9181 tttctcccgg ccaaattcat tcgggattgt ttcttattat ttggaattgg cttttcctta
     9241 ttcaagtcag atggtatctc gtgatcgttc ggtaatcgtg aattctttcg tagagaagaa
     9301 ccggatggct taaatttgga ttttgattgt tcctttgcca acttatttcc aactgctgca
     9361 ccaccaccaa aagccaaagt ccgtcccaca tttcgattga gttgccgcaa tgttcgattc
     9421 attaacattt gcggttttcg catgacccgt cttcccattg attggctatc attactctgg
     9481 agattgaaca tattcataat gtcgcccaat ttgaaataga taccggcaaa ggtcacaatt
     9541 tgcagaaaag caaccatgaa aaatggatag gttgccgaaa tattgaaaaa catcgtggag
     9601 atgctaaatg cagtcgtaat caccaaagta actcctgctc gcatcataat cgtattgaac
     9661 aagcgaataa tcgccttctt gcccaaactc tcataagtcg gcaacatgga caataagaaa
     9721 ctaatcggca aaaacatcgc aaagataata aagagaattt gcgagaacaa cataatccct
     9781 gttaggagaa agacaaagaa ggatatgcca atattgaaca acaccaaaaa gaccacggtc
     9841 cctaagcggt tcatggtctt ggtaatactg agattcgcat tgtcattatc ttcaatctcc
     9901 gctttgaccg cttcttcccg atccttgcct ttattttcat ctggacttac agacaggatt
     9961 ttgttaacac gatcttcgcc aacttcttct atattggaat catcaaattg caagagtaac
    10021 cacggttgtt ggacttgaat ggaaaacaag ctatctcgga ttaaatccac actgtccttt
    10081 ccttggcttt ctgaattcgg cacgacaatc tcggttccaa ggctaagagc cgcttcgctg
    10141 acatctgagc taaaatcatt gatttttgtg atataggtgg gcgcatacgc aatgaacgaa
    10201 cccgaaagta gaaaaatcat cacaaaatta atgacagccc ggactgcttt ggtagtttct
    10261 c
//
LOCUS       DACDRN010000083        10200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDRN010000083 DACDRN010000000
VERSION     DACDRN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.083"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            523..729
                     /locus_tag="IWJ80_002402"
     CDS             523..729
                     /locus_tag="IWJ80_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462088.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            925..1092
                     /locus_tag="IWJ80_002403"
     CDS             925..1092
                     /locus_tag="IWJ80_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9462089.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            1118..1462
                     /locus_tag="IWJ80_002404"
     CDS             1118..1462
                     /locus_tag="IWJ80_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9462090.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            1467..1748
                     /locus_tag="IWJ80_002405"
     CDS             1467..1748
                     /locus_tag="IWJ80_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462091.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            1851..2165
                     /locus_tag="IWJ80_002406"
     CDS             1851..2165
                     /locus_tag="IWJ80_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP9462092.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            2143..3837
                     /locus_tag="IWJ80_002407"
     CDS             2143..3837
                     /locus_tag="IWJ80_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9462093.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            3857..5035
                     /locus_tag="IWJ80_002408"
     CDS             3857..5035
                     /locus_tag="IWJ80_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9462094.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            4998..5684
                     /locus_tag="IWJ80_002409"
     CDS             4998..5684
                     /locus_tag="IWJ80_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP9462095.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            5684..6844
                     /locus_tag="IWJ80_002410"
     CDS             5684..6844
                     /locus_tag="IWJ80_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9462096.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            6854..7729
                     /locus_tag="IWJ80_002411"
     CDS             6854..7729
                     /locus_tag="IWJ80_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462097.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            7726..8037
                     /locus_tag="IWJ80_002412"
     CDS             7726..8037
                     /locus_tag="IWJ80_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462098.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            8027..8380
                     /locus_tag="IWJ80_002413"
     CDS             8027..8380
                     /locus_tag="IWJ80_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9462099.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            8370..8771
                     /locus_tag="IWJ80_002414"
     CDS             8370..8771
                     /locus_tag="IWJ80_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462100.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            8764..9168
                     /locus_tag="IWJ80_002415"
     CDS             8764..9168
                     /locus_tag="IWJ80_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462101.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            9180..9788
                     /locus_tag="IWJ80_002416"
     CDS             9180..9788
                     /locus_tag="IWJ80_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9462102.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            9808..10170
                     /locus_tag="IWJ80_002417"
     CDS             9808..10170
                     /locus_tag="IWJ80_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462103.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
BASE COUNT         3658 a         1486 c         2109 g         2947 t
ORIGIN      
        1 ttcaaatact ttatcatact tttgccctgg gttattaaga tcataactaa tcatgtaggg
       61 tttatacatg ttgtcttgca catccttgtt ttatatttca gcggaccact cgctgataaa
      121 taaaattata cgcttagtat ttattttcac aatattaatt tatcgtaata acttttgagt
      181 aaatagaaca aaaaacctgc actagtttcc gctagtacag gcagtgacta tatcggttga
      241 taatctagca tatattaaaa ataattgcaa gaaaagaaat ttatagtggt ttataccaaa
      301 ttatcaaagc aatgttattt tgttgctgtc tattgttttt taattattca cacgataact
      361 aaaggtgggt gaagagaaat gattccatta ataatttcaa tttttgcgct ctgtcttaat
      421 gtctatatga ttggatttaa aaatgggcaa aataaaaaat agtagcagcc aagaataatt
      481 ttatagtgtc actgtggcgg aaagggtaga cgctaagcat gtgtgctagg tcaatgcttc
      541 ggcaaccatg caaggttcga ttccttgcca gcgacattaa atgcctatga cggttacgac
      601 taccgaaaaa agatcgttaa gaagctatac ggtgctacgt acggcaatgt agtaagtgtg
      661 ctatctgtac accaccaagc ttcggtcact gtggcggaag tagaagacgc agcggtaaat
      721 ggcgagtagc ctcgtgagag cctggtaagt tctcgtgagt ggtgcaatcc cactccagcg
      781 actttaagca accgaggcat cggcggttta aaaatatagg ggtgcgcaat ttcgtacgcg
      841 ttttgtgcat cgtgcaagtt actattacat attagatcac tctttgagtg gtctttttat
      901 ttttgcacaa aggaggaaac aacaatgtat agaccacaat acttagaaca gaagtatgaa
      961 gtaatcactg ttcataaagg taatggcgaa aaagtatatg agtatagaag accaataaag
     1021 agcgatacat ataaacgaaa ggaaaacaat gaagttattc cattgtatgg caaaagaata
     1081 gctaagcatt aaataagatt gcgaaaggag acggaacatg accgaggaat tctatagatg
     1141 gctattacag ttgacaagag aagatcgttt ggttaagttc tatcagtctc ctaaatggcg
     1201 caggcttaga gagaaagcga tgaaacgaga tcactatgaa tgccaagagt gtagaagact
     1261 aggtaagtat catagagtag agaacgttca tcatataaag gaagtcaagg atagacctga
     1321 cttagcttta gatttagata atcttatttg tttatgtgtt gaacatcata atgaagttca
     1381 tggcagatat cttacagcat tagataaaca agagaagaag atagaaagct ttgctaactt
     1441 cgatgcaagt gaaaggtggt aagtgcatga tcatcaatga taatggcaga gagtatgata
     1501 cagaaaagat tgaagagtat tcatcttata ctcagggatt aattaaacgt ttgatatacg
     1561 ttcgctatgt aggtattagg gatctgttat cagataactg ttgtagtaaa tacaaagtga
     1621 atcaagtaag agaagcgttg aataaagata ataacgtcga aagaataaaa aatgtttttg
     1681 gatatagtat tgaagagatt aattattaca ttgacttcgc tgaagctttc attccgatgg
     1741 tgagataacc cccccttaaa ataaatcgca aattttttgg gggtgatgaa acggaggggg
     1801 ctgtcaggaa aagagatttt ttcgaacttt atcatgaaag gagggctaaa atgtttaaaa
     1861 acgaattgtc tcaaaatcgc tacagagaaa aattacgccg ctctttaata agccaattgg
     1921 aaagtcagaa aacaaatatt gagccattct tagataatgt tgatcgttat atcagtttat
     1981 gggaaacggc gatatcactg gaagaagata tatccgagaa cggcattaga ctggagaatg
     2041 gtaaaaagaa tgaatcagta gcgttgcttg tttctgtcaa caaacaaatg ggattgatgt
     2101 tggataaact tgccattact cctgaattgg taggtgaagc aaatgaatca attcctgagt
     2161 tataagcata ttgaaaattg gttcaaagct atagaagaag gcactatcaa ggtatgcaaa
     2221 gagcaattat tgctaaaaaa ttatctagaa gaaagagtct ttactagaga agatatttac
     2281 ttcgataagc agatggtaga ggattcaatc aatataccag cacaatactt tccattcgaa
     2341 ttaattccgt gggaaaaatt tctacaatgt tttatttatg gtgttcgatg gaaaaaagat
     2401 aaaacactag tgttcaatag atatctttca ttaatgggac gtggtaatgg taaaactggt
     2461 tttgcttctt ggaacaactt ctttctacta accgctaaac acggtattaa aaattatgat
     2521 attgatatct atgccaataa tgaaagccaa gcaaagacta gttttgatga tgtatttaaa
     2581 gtaattaaag atcatcctga tttagataaa aaagtattta aagctacgaa ggaagttatt
     2641 caaaatatcg ctacaaacag caaacttcgt tataacacgg caaatgctag aacaaaagat
     2701 gggaagcgac caggtgcaaa ccgctttgat gaaattcacg aaaatgaaga ttattcaatg
     2761 ataaatgtgg ctacttctgg tggtggtaaa attcgagatt atagagaatt ttatgataca
     2821 actaatggtc atgttcgtgg tggtccgctt gatgacatta tagaagaatc aaaaatgatt
     2881 ctttctggag aacttggaat tgacaaggat ggagcagaat tttctagttt gtttccattt
     2941 atttgtcgct tggataacga taatgaagtt gatgatcccg acatgtggga aaaagcttgt
     3001 ccaactatta attacaatgc agatttaaaa cggaaaatgt ttcaagaata ctctcaaatg
     3061 caacgtaatg ctggtttaag acttacgttc atgaccaaac gaatgaacag acctatggaa
     3121 gatacacgat ttgctgttgc ttcatatgat gatgttctgc atacgaaaga aaaagaattt
     3181 cctgaaaaaa tggatgaagt gataggaaca gtcgattttg ctgatagacg agattttgcc
     3241 agcgttgggt tgctaggaaa atacgataaa gatgtttatt ttacacaaca tacttttatc
     3301 cacgaatcag cccttcgatt acaaaacatc aaacgagagg ttatagatat ttctatagat
     3361 caaggaaaat cacagatcgt tcatggaaaa aatatagaag ctgattatat tgtaggttgg
     3421 tttcttgaaa tgagtaataa atattatatt aaaaaaatcg ctatggatat gtaccgtgca
     3481 aaaatattga agcccgcttt agaagaagca ggttttactg tggaaattgt tcgaagcgga
     3541 tctgttacac atggtatgtt aaaagatctg gttgatgacc tttttattaa tcaacgttta
     3601 ttttttggtg acgatgcgat tatgcgttgg tattgcatga atgtatatga agagcatatt
     3661 tctaatggaa atatacgcta tgaaaaaata gaacctgaaa ctagaaaaac ggatggcttt
     3721 ttttcattcc ttcatggttt gaatttttta gatgatattt atgattctgc tcctgtaaca
     3781 gtcacaaata gctcagtaga aaatacagga actggattta ctcctctagt attctaactt
     3841 gaaaggaggt gagaaagtgg ggatttttca aaaggcggta ggatacttca caaaaaaagc
     3901 aacggttcct ttagaagaat acttttgtaa attgcaagtt gattttgtgt atcgaaaatt
     3961 tgcgattgaa acttgtattg atttgattgc aaatgcgatg agtaaagcgg aattcaagtc
     4021 atatgaagat ggaaaaaata aaaagaatga tctttactat aggctgaatg tagctcctaa
     4081 taagaaaaat aatgcaacag aatttagaaa aaaactgatc aggagattaa tattctacaa
     4141 tgaagtattg atcgtttctc cgtctaataa ttctagcgaa atatttattg cggatagttg
     4201 ggatgtcaca gaatatgcat tgaaagatga tgtgttttct caagtgcaaa ttaacaacat
     4261 agtccttgat agagaatttc tagaaagtga tgttatctat ataaaatacg cagatcaaca
     4321 aattaggcaa ctagtcgatg cgtattatca agcgtatggg aaactcattt ctagtgccat
     4381 gaatgtttac aagcgctcta acgctcgtag atacgtactg aaagggaatt tattccgacc
     4441 gcaagacaat acaacacaag atcaaatcaa taaaatgatg acatcacaat ttaaggcttt
     4501 tatggaagct gataatgcag gtgcggtatt tcaattacaa aatgagtaca cattagaaga
     4561 tttcagcgga aacttccaaa gcaattcaag agatataaaa aacttaatag acgacatctt
     4621 tgagatgaca gcagcagcgt ttcacgttcc gaaaaaccta ctaaagggag acatgagtgg
     4681 gttatcggat caagtggacg cttttttaat gttcgaaatc ataccgattg ctgaacttat
     4741 tcaggatgcg tttaacgcta gtctctatga agtagaagaa tacttgtcag ggaattttgt
     4801 acgtgtggat acaactatga tcaagattac tagcttcaaa gatttggttg acgctattga
     4861 tgtaggcatt agaaatggag tatttacaat caacgaagga agagaacgcg ttggaaatga
     4921 tcgctctgat aaggcgatgg cagatgaaat atttataact aaaaacaatc aacaagtatc
     4981 gaaaggaggt gaggcgaatg acgacaatga aaacatttct agcagtaaag aatgaaggcg
     5041 cagtaccgca aatttttatt cagggattta ttggttctag ttggttcttt gaagggaata
     5101 ctgacaaggg aatcaaaaat attttggata gtctaggtga tcaagaagaa attgaagtag
     5161 taattaattc aaacggtgga gacgtatttc aagggattgc tattgggaac ttacttaagt
     5221 caaataaagc aaaagttaac gttgtgatta acggcttagc cgctagtgct gcttcaatta
     5281 tcgcaatggc tggcgatact ataaaaatct acaacaatgc acaattgatg attcaccgcg
     5341 cttccacata cggagaagga aatgtcgatg acttccgtac gattgctgac caactggaat
     5401 caattgataa atcggtaaag gcttcatata aaacacgatt caatggcaca gatgaagcat
     5461 tgcaagaact tcttgaaaaa gaatcgttta tggatgcaga aacagctttg agttatggat
     5521 tggtcgatga aattatcgat gcagaaaata gctcaggtac tgaagctaaa aaagaacaaa
     5581 gcgttgaaga aattttgaat gacgttaaag aaaaaagagc agaaaaaatt gctgcattta
     5641 cagcagcatt aaataaaaca tttggacaag gagatgtaaa ataatgacag ttaaaaattt
     5701 aaaaggtgta acagctgcaa gcgaccaatt gatgaaagct tttaaagatg gtaacgaaga
     5761 atcttttagc gcagctatgg taagcttatc taaggaaatt caggataaaa ttttagaaga
     5821 agcaacagca aaaaatcaag atcaattagt attaatgaac cgtggtcagc gtgtattaac
     5881 tacacaagaa acaaaattct ataacgaagt agtgaaaaac gaaggttttg caggggtcga
     5941 agaattagtg ccagctactg tatttgaacg tgtatttgaa gatttagaac aatctcatcc
     6001 actattgcaa aaaattactt ttgttaacac aactggtgta acagaatgga ttgtgtcacg
     6061 tggagtcaat ccagcatggt ggggtaaact ttgcgaagct gttaaaaaag ttttagataa
     6121 tggctttgat gtaattaaca tgaagcagtt caagctatca ggttatattc ctgtatgtaa
     6181 ggcaatgctt gatttaggtc cagtatggtt agatcgttat gtccgtactg ttttagtaga
     6241 atcattgaga attgcattag aacaagcaat tgttgatggt actggtaaag atatgccagt
     6301 cggaatgatg cgtgacatga gcaaacaaac tagcggagaa tatgctgaaa aaaaagcaga
     6361 acctattaca gctttagatg ctgtaactat gggcggtttg atggcgcgac tatcaaaatt
     6421 caatatcgaa ggtgtgaatg atccgattta tcgtaatgtg aatccttctg atgtggtcct
     6481 aattgtgaat ccaactgatt actggtctaa agtattccca gctaagactg tactaactgc
     6541 taatggagaa tacgtacaag tattgccagt accagtttca gatttgcagt caacggctgt
     6601 gccagaagga aaagcagtta ttggggtagc ctcagattac ttcatgggtg taggatctac
     6661 actaaaaatt gaagcttcag atgaatacca ttttgttgaa gacgaacgca tttatctagc
     6721 taaacaatat gcaaacggtc aacctaaacg taacgatagt ttcattgtat tagatattag
     6781 cgctttggga actactacta caactacaaa accaacaacc acaacaacta caacacaagc
     6841 gtaggtgatc agaatgaagt atattctttg tcagccggca atcaatcggt ttaaatggga
     6901 gcttgaagtt tgtttaacta atctgaagaa actaggaatc aaagatatcg tattgctttt
     6961 cagcagacac gatgatcaga ttcctatttt ttttgagaag gaatatggtg ttgaagttca
     7021 tgtgtacgat gatctgcggg acgacaaaga gtatattcct tcgattaaac catatttatg
     7081 gtggaaatat ttagaagaag atcattcgcg tgaggacgac cgatatttct atatcgattc
     7141 ggatgtcatt ttcaataaaa gaattaattt gcgcaaattg ccttctaaag atgatgtttg
     7201 gtattgtagc gactgctgta gttatctaag tcttgattat attagaagct gtgaaaacgg
     7261 agaaaatatt ctaaaagata tggcaaacat tgtaaatgtt acagtagaat ctttggaaac
     7321 tataaacact aattcaggag gcgcacagtg ggttattaac cgtcctaaag cgaattattg
     7381 gaaaaaggtt tatctggatt ctaatcggct atatcgctac cttagagggc aaaaaacaaa
     7441 tatacaaatc tggacagccg agatgtgggc acagctttgg aacatgatgt atttcaatat
     7501 tggtcctaaa gttcacgagg aattagactt ttgttttgct actgatccaa tagaaaaagt
     7561 taaagaagta aaaatcttac acaatgctgg agtaacaaca aacgatgaag atttattttt
     7621 caaagggaga tacgtgactt ctacgccttt tgatgaagat ttatcatttg taaacaagaa
     7681 aaaatgctct tacgcatatg ttaaagcaat taaggcggtg gttagatgac gcctgaacaa
     7741 gtgactgaag aattgctaat agctgtgaag gataatattt acgttacctg gaacgaagaa
     7801 gatgagtcaa ttaaaaagat gatagctaaa aatgctgttt atcttcaaag taaagtgagt
     7861 acaacacttt ctttttctcc tgaaagctta gaatacggat tgctaatcga aagatgtaga
     7921 tacgactgga atcgtgcttt agatgagttt gaacaaaatt tcgctagtga gttattaggt
     7981 ttcattcaac attatgcgct acaagaatat attgcaggtg atgtgaatgg cgaataatcg
     8041 tagactcgaa gaaacattca acgatggttg gttaaagatt ttgacgcaaa ctaccaaaag
     8101 aaatgaacta ggaaaaaaga ttggtgtaga agatacagaa atcacttctt taaaatttag
     8161 aaatctttcc atgagagata gtgatataac agctatggat gcgatgggat cgaaattaac
     8221 taagaaagta aagactccat ttcatccaat cgccaagaaa tttaataaag atcaatattt
     8281 tatcgtaatc gatagtatgc gttacaacgt tatctatgcc gattacgata atttttatat
     8341 ctatttttat cttgaaagtg tgggtgaata tggtgattga taattctaaa gaaaaagaac
     8401 gtttaaataa gcaaatttct gctatcaaaa cttccttaga agagcatttt ggcctcaaac
     8461 tctttcaaga ctccgtaggc gaggatgagc tacctgatga ttttaattac ttcattctcg
     8521 aaacaggaga aatagaaatg atcactgagc caaaatatag cgtgggtcaa aatctatatc
     8581 taactttcta ttcagaaaat agagaagatt taacaggaga ctcactagat attatttcat
     8641 tgattcaaaa tcgttcgatt cgttttcaga gaatggatcc caatcattta aaactagaga
     8701 accaagatcg ctatatcgat caattggtat ttacgtttag acgattattg aagagtgatt
     8761 gtcatggcta aaaatagttg ggagctaaaa ataaatggac atgatgaact tcttgtgcgg
     8821 atggaacgct attcaagcga gagcgaacga ctgattaacg aagcattgaa atcaaaaggt
     8881 tcggctattg cagtggatag gattacagaa aaaattcctg tttctgaagc agatttaaga
     8941 agagggcacc aacacgcaaa aaatagtcgt ccacttaaga ctcaatacat taatttgggt
     9001 ttcatcatta gacctacaag aaaatttgag tatttaaaat atcctgattt ggggataggt
     9061 acttctaaaa gaaatcagcc ggacgaattt atgagaagag gattaggtct tgcacttgat
     9121 ccaattacag aacttctgat tcgtcaattc gataaattaa ataaataggg ggaacaacaa
     9181 tggctaaaac aaaaactgta gtaacaacgt tcgataacgt aagtatcaaa cgaattgctt
     9241 ttaattttaa gaacgcagga aatgcaatcg caacgaattg taatggacaa ttagatggcg
     9301 aaacagaaat gcaaacggtg gttaaaaaat gtggagcgac agaagtaaaa tcaaaatcta
     9361 aaccaatcaa tatgacggta acaattactg cacatgtacc gatggaagtt tatcgacgtt
     9421 tcaatgggtt gaaacaagat ggacgtatta aaccaggcat ttactcttac ggtcctgatt
     9481 ccgtaggcga agatttctca cttgctgcag agatcgtgga tgacttcgaa gaaaatagaa
     9541 agttagttgg tatgttagca tgcacttcga atacaggatt aacattctct attgaaaatg
     9601 gtgcggatga agtagctgcg ttagaactag aaacaaaagt tatgcaagat gaatttggta
     9661 aattttatca tgaagcaatt gttgcagaac ttgaagaaga cttaacagat caatggatga
     9721 cgaatctatc tgctgatgtg attaaaaaaa cttcagttgt gacaactacg gccactcaat
     9781 cacagtaaaa aaaacggagg tagcgaaatg aacgaagatt actcaaaaat tgaactaaac
     9841 gatggaacaa ttttgaattt agaacctaaa ctgaatatca agaaattatt gatgatcaat
     9901 agagatttta acacagacga gtttgcaaaa atgactgtgg gaaaaggatc catggatatt
     9961 tctgttattc aaggtgcaaa ggctgtgtat attgcttacc gccaagcgaa catgactgat
    10021 tatatttcat tcgatgaatt tatcgataaa tgggattttg atatggctac tgccagctat
    10081 atttatcaat tgatgatgtt caaacaagca cgcgatgctt atcaaaaaga atttgaaaaa
    10141 gcaaataagg aaaaaaagct tcaaaagtaa aaattccaaa gctcttagtt gaaacgtggg
//
LOCUS       DACDRN010000084         9939 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDRN010000084 DACDRN010000000
VERSION     DACDRN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.084"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(326..1174)
                     /locus_tag="IWJ80_002418"
     CDS             complement(326..1174)
                     /locus_tag="IWJ80_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9462104.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1338..2240
                     /gene="gnd"
                     /locus_tag="IWJ80_002419"
     CDS             1338..2240
                     /gene="gnd"
                     /locus_tag="IWJ80_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP9462105.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2242..3243
                     /locus_tag="IWJ80_002420"
     CDS             2242..3243
                     /locus_tag="IWJ80_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9462106.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3240..3524
                     /locus_tag="IWJ80_002421"
     CDS             3240..3524
                     /locus_tag="IWJ80_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9462107.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3557..4354
                     /locus_tag="IWJ80_002422"
     CDS             3557..4354
                     /locus_tag="IWJ80_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9462108.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4368..5201
                     /locus_tag="IWJ80_002423"
     CDS             4368..5201
                     /locus_tag="IWJ80_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9462109.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5219..6076
                     /locus_tag="IWJ80_002424"
     CDS             5219..6076
                     /locus_tag="IWJ80_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9462110.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6098..6607
                     /locus_tag="IWJ80_002425"
     CDS             6098..6607
                     /locus_tag="IWJ80_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9462111.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6644..7045
                     /locus_tag="IWJ80_002426"
     CDS             6644..7045
                     /locus_tag="IWJ80_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462112.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7157..7510
                     /locus_tag="IWJ80_002427"
     CDS             7157..7510
                     /locus_tag="IWJ80_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462113.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7544..7987
                     /locus_tag="IWJ80_002428"
     CDS             7544..7987
                     /locus_tag="IWJ80_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9462114.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            8004..9089
                     /gene="uxuA"
                     /locus_tag="IWJ80_002429"
     CDS             8004..9089
                     /gene="uxuA"
                     /locus_tag="IWJ80_002429"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9462115.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9082..9795
                     /locus_tag="IWJ80_002430"
     CDS             9082..9795
                     /locus_tag="IWJ80_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9462116.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3322 a         1569 c         2180 g         2868 t
ORIGIN      
        1 acctaaaatg aggccaaaag ccagtgttat caaggaaata cttaattcac caaaattttt
       61 tttaattttt tagaaaataa gaaacaaaaa taataaaaaa aagaaaagat aaatttttta
      121 ctactgtata ctaactttgt tatcgcatct aacaagtaac tcgaataata ccaatacaac
      181 cttctccaaa gatgcgatga catacgttct aacccaccga ctattgatgt tctgtaccac
      241 ccttgcagaa ccaaaaaagc cactaccaaa tatttggtag tggctttttt tgttttatgg
      301 atttttcgct ttttctgtta tggtgctatt ctttgatttc agaaatagta tgtcgaactt
      361 tagcgataga acgacgtgat tctttttcgt tgtaaaacat caaaatcaca tatagagagt
      421 ccaaaatgct tagttgagaa atacgggaag ctaaagcttc agatcggaat tctgtttctt
      481 cagaaataga aataaaaacg acatcgccaa gtttagcaag ggaagaatta gcttggctag
      541 taataacgat tgtttttgct ccagtttttc ttacggtttc agcaatatgg attgtttctt
      601 ttgattgccc tgtgtgagaa atcaaaacag cacagtcttt ttccgtcaat aaagaagctt
      661 ccatcaattg aatatggtaa tccgtgctgt gagaagttgc gataggggaa cgcaaaaatt
      721 tatgataagc gtcagttgct acaatctctg aaccgccaac accgaaaaaa tagacacgtt
      781 tagattcatt gataatagta gccgcttttt ttagatcctt ttgttgtaac aacttttttg
      841 tgtcatttaa agttgaaata ctagactcga acaccttttg cgccatagtg agctcagtat
      901 ctgttttctg aatattttcg tggatagaaa tagcagatag atcattttct tggatcaaca
      961 tggccatttt aaaatcttta aatccattat aaccaagttt ttttgtaaat tgaaaaaaag
     1021 tagagtcagc aatgcccagt tcacttgcta aattgctaat aggactatga cttactttgc
     1081 ttggattttc taaaatataa tcggcgatag cctgttcttt tgggctgaag tcgttgtaca
     1141 ttgttttgat ttgtagtctt atggagacgc tcatgagaat tcctcatttt cataataaga
     1201 ttatcatttt ttccgttctc attaatgtta aagatacaag gagagtagga aaaaatcaaa
     1261 caaaaaatat ttttgtaaaa tctatttaca aaaaaatttt tttgtataat gagggtataa
     1321 gaaatggagg cgctatcatg gaaattggtg taattggttt gggaaaaatg ggattgaatt
     1381 tagctttaaa cttaaaagag caccatcaca aagtacaagg actcgacatt tcaagtacag
     1441 ctgttagtca ggcaagagaa aatgacattc ctgcttatca agagatctct gaatttctag
     1501 ctacttttga gcagaaaaaa atcatctggt taatgcttcc tgctggagag ataaccgaaa
     1561 acatgttaag aaaacttttt ccaaaactcg gaccaggaga cattattatt gaaggcggaa
     1621 actcttatta taaagattca atccgtcgag cagaagagtt tcagaaaaat gaaatcggtt
     1681 acttggattg tgggacttct ggaggaatcg aaggagcacg acatcaagcc tgcttaatga
     1741 ttggaggaga taaagaaact tttctatccg tggaacaatt attcaaagat gtagcaattg
     1801 aaagaggata tctgtatgct ggtgcatccg gaagcggaca cttcttgaaa atgatccata
     1861 atggaatcga atatggaatg atgcaagcaa tcggtgaagg attccaactt gtacaaaaaa
     1921 gtgagtatgc ttttgacttg gaacaagtcg cacgtgtttg gaatcatggt tcggtgattc
     1981 gcggctggct gatggaaatc atggaagatc agttaaacga acatcccaat ttaagtaatt
     2041 atcggggaat cgtttctgca tcaggtgaag caaaatggac ggttgaaaca gcgctagaaa
     2101 tggctgttcc agttccagta attgcaacta gtttgttcat gaggaatctt tcacaggaag
     2161 aagatagttt ttcagcaaaa gtagtttcag cactaagaaa tgggtttggc ggacatgaaa
     2221 tagttgaaaa gggtgagtaa aatgccagat tatcatatag caatcgttaa ttccagcagt
     2281 ttcggaagag tctttccaca ccacttggaa agattaaagt cagttggaga cgttcatttc
     2341 tttactgttg atcaagagat tccaggaaaa gaactagcgg agttacttaa aggattcaat
     2401 atcattattg caagtgttac gccatttttt cgacgagatt tttttgaaaa caaagatgaa
     2461 ctgctattga tttcccgaca tggtattggt tacaacaata ttgatttaaa agcagcaaaa
     2521 gaacaccaga caatcgtatc tatcatacct gctttagttg aacgaaatgc tgtagcagaa
     2581 aataatataa ctaatttgtt gtccatgctt cgacgcacga cacaagcaca agcaagagtc
     2641 aaggacgatc gatgggaaga tcgggcggag ttcgtcggta ggacgctgtt taacaaaaca
     2701 gttggtgtga ttggtgcagg aaataccgga agcggagtaa tcgaaatttt gcgtaacggt
     2761 tttcgctgtg atgttctagc ttatgatcct tacaaatcag atttatacat cgaaagcttt
     2821 ggtgcaaaaa aagtagaact tcaagaatta ctggaacaat cagatatcat ttgtttatgt
     2881 gcgaatttga cagaagagaa ctatcacatg atttctgata aagaagtcga tcagatgaaa
     2941 gaaaatgttt atctatctaa tagtgcaaga ggcgctctag ttgatgaaga agcgatgatt
     3001 aatggtctga aaagcggaaa gatcgcagga tatgccacag atgttttaga agaagaaccg
     3061 ggacgtcaag atcatccgta tttagcgttt gaacaagtag tgattacgcc acatatatcg
     3121 gcctacacaa gggaatgttt agaagcaatg ggtgaaaaat gtgtcagaga tgtcgaacag
     3181 atcagtcagc accttcttcc tgaacgttct gtccagacaa agagcaacta tataaaagga
     3241 tgatgaagaa aatggtgaac ggtttaaaag tgaaaacagg ccctcaattt tatttatatg
     3301 aagaaggtgg tatttcaaaa gtttctgatc tcttgaaatc atacggcgcg aaaagagtac
     3361 tggtcaccca cggaacggta tcatgggaaa aggcgctacc taaattagta tttttgaatg
     3421 acgaaacgat tcaatttttt tatcatcgtt attctggaga atgtagttat gctgaagcca
     3481 gacgaatagc tacaatcatc aaaaaaatga aatcgatttc gtagttggcg ttggcggagg
     3541 aaaactgacc gatctagtgg gttatagtgc caatcttgca agaatcgatt tcggagtcat
     3601 ccctacttta gctagtaatt gtgctccatg gacacctctt gctgtcatgt ataaggagaa
     3661 tggacaagca gaagggaaaa cagaacactt ttacagacaa gctgcttttt ttataacaga
     3721 tccccaatta gtgattgatt cccctgttcc atatttcatt gccgggttag cagatacact
     3781 agcaaaatgg tatgaatctg agctcatctt agaacaagat tttttgcaag atgaatcatt
     3841 tttgaagcta gcacaagata ctgcgaagat ctgcaaagaa gagattttga atcattctgc
     3901 taaagcaatc caagatatgg cacaaagaaa actgacatcg gagttcaaac agctttcaga
     3961 gattatcttt gctgttgctg gtttagttgg aggattagga gataaatacg caagaaatgt
     4021 ggcagcacat gctatgcatg acgctatcag taaatatatt ccggaaagcc atcgttttct
     4081 acatggagaa aaagtagcgt atgggatttt ctatcaactg gcactagaag gtcgctggga
     4141 aaaaatagac gagttatatc ctttgtatcg ggaattagaa ctgccggttt ctctagaaca
     4201 aatgggatta tttccgaaag ataaacagat aatagataaa atggctgaat ttattgattc
     4261 gaaagagaaa gtgcatttga ttcctataga ggtaacacag aaatcactga tacagtcaat
     4321 gatggaactg gaaggttata tcagaaagat ctgaaggagg aggacacatg gaaacattaa
     4381 gtgtagtaca aagtttgttg attgctttat gggtagccgc ggttatgtcc cgctggttag
     4441 gtggaggagc cacgttgacc ttacgttttt ctcctttgat gactggatta gtcgtcgggt
     4501 tgatcatggg ggaagttgca caagcgatga ttgttacagc cgctttgcaa atgatttata
     4561 tgggggtttt ttctccagga ggatcaatgc cagctgaacc atctattgca gcagctatcg
     4621 cagtaccagt cgcattatta ggggatctgt cgccagaagc agcgattgcc gttgcggtgc
     4681 cagtcggatt gctcggaagt tacttgtatc agtttcgttt tttcatcaat acttttttag
     4741 gtaaatatac agacaaagct gtagaagatt tgaatacaaa agctatggcc cgatcaatta
     4801 ttctttatcc aacggttgcc tcatttttat tattcgttcc gcttgttttc attgctttat
     4861 atttaggggc gccattgatc gctgatgtaa tcaaagcttt agagggaaca gtcgttatcc
     4921 acattcttga agtagtaggt ggagggttag cggcaatcgg tatcgcgaca actgtatacg
     4981 ttatcggtcg aaaagattat ctggtctttt tcttccttgc ttattttatg agtgttgttt
     5041 tgaaatcttt agatattacg atggttactt atgccatttt tgggattatt atcgctttga
     5101 tctttgtcca gtcgcaaaaa gggaaacaag tacagcctgc cacaagcaat aacaattcag
     5161 catcttttga tgacgaagat gactatgatg acggatttta atttaggagg aatgggaaat
     5221 ggctgaaata atggataatc aaacaggtgc tcctgaagaa atcacgaaaa aagatgtaac
     5281 aaaagcctat ctgagatggc attttgcaaa cgaaattcct cactcttttg aacgttactt
     5341 ggcgccatca ctactatacg gaatgatgcc aatcttgaaa aaactttata aagatgaaga
     5401 ctctttaaaa gcagcatata aacggcagtt actgttcttc aacactcaat taagctgggg
     5461 tggcggtgtg attactggcc taatgtcttc tatggaacag caaagagcag aagaagagta
     5521 tgaagaaaaa gagatcttga tgcaggatga cttgatgtat aacacgaaag cgggattgat
     5581 gggggcacta gcaggaatcg gagatgcaat cgattcagga accgtccaat atatttttat
     5641 cgcaatcgcg gtaccttggg cacaacaggg aagtccttta ggcgcacttt ttccttttat
     5701 ctgctttggg ttgtatcagg ttttactagg ggtatttttc gcaagacaag ctttcactat
     5761 gggccgtaat gctacaggcc taaagcaaaa cactggtgtt cagacagcga ttgaaatgct
     5821 atcagtttta gggttgttta tgatgggggt gcttgcggga aactatgtaa aagtagaatc
     5881 ttcattgaaa tttgcgatat ccggacgtga gtttgttatc caggaaattc ttgatcaaat
     5941 cgtgccaggg atattacctt tagctgtcgt aatgggtgtg tacttgttct atacgaaaaa
     6001 aggattgaaa gtcacacaag cattattatg gctgactgga atattaattg tgctagcagg
     6061 aatcggtatt ttataaaaag aagggaacgt gaataagatg ggtgtagtca atttagcgcg
     6121 tgtggatgag cgtttgatcc atgggcaagt aatggtaacg ttatctcaaa aaagtggagt
     6181 gaattccatt tttgttgtag acgaagttgt agcaaaagat aaatttatga gagatcttta
     6241 caaaaatgca ggaagccgta caggacaaaa aacaattgtc attacaccag agaaagctaa
     6301 attctattgg gatgaatatc aatttaaaga atacaattgt attttgatcg caaaaacagt
     6361 ccaaacgatt tacgacttag taaaacacgg aatccccatg gaagaattga atattggagg
     6421 gattgctcaa aaaaatcctg atacagatct tttagtgacg aaatctgttt atttaaataa
     6481 atcagatgct gaaaaattga aagaaatgaa tgaacaatat ggcgtaaaga atatttattt
     6541 tcaagccact ccatctgcac ctaaaacagg attaaaagat gtattgaaca agtttgattt
     6601 gtcataaaca aatgcttaga aaaaagataa agagggcgaa aaaatgaaga agaacgagct
     6661 gtttcgtgat tgggaatttc gttatcgcta tatttaccgt aagcgccgta caaaaaagag
     6721 taaacagcgt tttctttcag cattagtatc agatatttac agtatgagaa cagatgtgac
     6781 agtcattgct tacgatacgc tagcctaccg atcaaaaaat atttatgttg gagatatcga
     6841 aaaagcagag aaagttatct gtacttatta cgatacaccc gttcatgcat taggaagtta
     6901 tttcatgttt gattggaaag accaaaggaa aaaaacaatc tactctatat tattgtcctt
     6961 cattttgttg ttcagtttag gttggtgggg aatgatgata tataacaaaa atcctcatca
     7021 cgtattcgat ctgctatccg tataaacagg tatcactgta ctagcatttg ggagctactt
     7081 tttcttattg ggaaaagcag ctcgcggatg gcctagcaga caaactttta ttcgaaatac
     7141 ttcatccata ttaacgatgc tagaaatgat ccgtacgatt gatgatccga gtgtagcgta
     7201 tgcgtttgtt gatgaaggct gttatggaga aaagggctta gacagcgtga gatcgggtat
     7261 gaaaaaagag gctattcttt tttatctaga ctctgttggg gcagatacac cgctacagtt
     7321 ttcgggaaat tatttttcaa ataaagaaca gtggctgaaa caagttgata agttgaaaga
     7381 aaaaaacgtc aattatattt ttagtgcaag aaaaaagcaa gcgcagttct tctatttgac
     7441 caaaacagat ttacgaggga aaacatttaa ttggcaaaat gccaatcaga ttattgctct
     7501 ttttcgataa gtaaaaagtg caaaacaagg agtgagaaga ctaatgttgg aaatcatgat
     7561 cgttacacat ggtaagttga gcgaaggact gaaagatgcg gcggaagtaa ttgtcgggac
     7621 atcgagccat attgaaacaa tgtgcttaga gtctggggag gacatccaaa cattcggaga
     7681 gaaggtcaaa gaaaaattaa ctatgatcaa cacagcagaa cccatgctag tatttgttga
     7741 tctaattagc gcaagtccgt acaatcaagc gctcttggca gtcaatgaac tgactgaaga
     7801 aaagaaagca gccacttatg tcataggggg cgtgaatctg ccaatgttgt tagaagcaat
     7861 caatcatcag atgcttcaca caccgataga agaagcggtg gaacaaattg tcaaacaagg
     7921 aaaagataat ttaactgttt ggcatgcttc agcggaagaa aaaaaattag atgaggacga
     7981 tttttagaag gaaggctgaa aagatgaagt ggggatttag atggtatggt gaaaaagggg
     8041 attcgatccc attaaaatat gttcgccaaa taccgggaat aaatgggata attggaactt
     8101 tgctgactaa aatgcctgga gatgtttggg agatttcaga aattgaggag ttgaaagcat
     8161 ctgttgagag ccaaggactg gaactgcttg gaattgaaag tgtagcgatc cacgatgcaa
     8221 tcaaggcagg gacagatgaa cgagattact atattgataa ctatatacga acaatcaaaa
     8281 acttgtctcg atgtggggtt cgattgattt gttatagctt caaaccaatt tttggatggg
     8341 cgaaaactga tttgtttaat gaaaacaacg atggaagcta ttctctttta tacgatcaga
     8401 aaatcgtcga tcagatggag ccaggagaaa tgtatcaatt gatacacagc cagtcaaagg
     8461 gattcaaatt gccgggttgg gaagaagaac ggttgaaaaa attcgctcag ctggaaaaac
     8521 tgtatgaagg aatgaccgaa gaagaattat ttgaaaatct gacttatttt ttagaaaaag
     8581 tgattccagt gtgcgaaaaa gcagatgtaa aaatggctat ccatccagat gatccgccat
     8641 gggaactttt tggttttcct agagtgacga aaaatcttgg cgacttgaag aaaattctaa
     8701 atgcagtaga ttctccatat aatggactaa cgctgtgcac aggatcttta ggggcaaatc
     8761 cggaaaacga tttagttgaa atcatccatg aggtaggttc gcgcatccat tttgtccact
     8821 tcagaaacgt ggagttttta ggagaaagaa agttcaaaga aacggcacat ccctctacag
     8881 aaggctccct tgatatgtat catattatga aagcgttggt gcaagaaggg ttcgacggtc
     8941 ctatacgccc tgatcatggt agaaccgtgt ggggtgaagt ggctatgcct ggatatggcc
     9001 tgtatgatcg tgcaatggga ctcacttaca tgcaaggtct atatgaggcg ataagtaagg
     9061 aaaaaccact gggagagcat tatgaataaa atcaagctct tcatctttga tatggatggc
     9121 cttttattag aaactggacg tttagcttat cgtgcttatg taaaatcagc acaaaagtat
     9181 gattatgaaa tgcgtaagga agtctattat ttactaactg gccaaacaga aatggcgata
     9241 agagaacaaa tgggcatttt gtacggagaa gatgttccat atatcaaatg gcgagaagca
     9301 atcaatcaat ataaagaaaa aatcgtgaaa gaagataaga gagtatatac gaaaaaagga
     9361 gcagaagaaa ttttatcttt tgcaaaagaa agaggaatcc atacaatcgt ggcttcgtct
     9421 aatacacggg taaaagtaga aatgtattta aaaatggaaa atctatatgc cttgtttgat
     9481 caaatcattt caggggatga tgtaaaaaaa ggaaagccgg aaccagagat ctttttgaaa
     9541 gcttgttcaa aaatgaatat tcctccaagt gaagcacttg tatttgaaga ctcgattgca
     9601 gggatcgaag cagcaaggcg agcgggcatt ttgtcttttc tcatcaaaga tcatttgata
     9661 gggttgcccg atcataatgg gaagcataag ttgaaagtag atgtaagttt atatgaatcc
     9721 aataaagcag actatgaatt ttatgacctg catcaagcaa aacgattttt agaaagtaaa
     9781 gggttgtatc tctaataaag aggactattt tctagtcctc tttatttatt gtcctgcacg
     9841 actgagggaa cagatgaaaa agaagtaatt ttttattttg aagtatctac cttatttgta
     9901 gtgaacaatt tagaacaaac acaaaacgca tctactgtt
//
LOCUS       DACDRN010000085         9929 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDRN010000085 DACDRN010000000
VERSION     DACDRN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.085"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..357
                     /locus_tag="IWJ80_002431"
     CDS             1..357
                     /locus_tag="IWJ80_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462117.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            373..723
                     /locus_tag="IWJ80_002432"
     CDS             373..723
                     /locus_tag="IWJ80_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAP9462118.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            752..1531
                     /locus_tag="IWJ80_002433"
     CDS             752..1531
                     /locus_tag="IWJ80_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAP9462119.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1542..2219
                     /locus_tag="IWJ80_002434"
     CDS             1542..2219
                     /locus_tag="IWJ80_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAP9462120.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2238..3344
                     /locus_tag="IWJ80_002435"
     CDS             2238..3344
                     /locus_tag="IWJ80_002435"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAP9462121.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3334..4425
                     /locus_tag="IWJ80_002436"
     CDS             3334..4425
                     /locus_tag="IWJ80_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAP9462122.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4438..5178
                     /locus_tag="IWJ80_002437"
     CDS             4438..5178
                     /locus_tag="IWJ80_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAP9462123.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5466..7406
                     /locus_tag="IWJ80_002438"
     CDS             5466..7406
                     /locus_tag="IWJ80_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9462124.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7493..9415
                     /locus_tag="IWJ80_002439"
     CDS             7493..9415
                     /locus_tag="IWJ80_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP9462125.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(9695..>9929)
                     /locus_tag="IWJ80_002440"
     CDS             complement(9695..>9929)
                     /locus_tag="IWJ80_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP9462126.1"
                     /translation="DLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQ
                     AVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         3239 a         1648 c         2205 g         2837 t
ORIGIN      
        1 atgattaaag tagttattgc agaccgtatg ggtaaaggac aaaatgtggc caaaggggta
       61 gaagcagctg gtggaaaagc agttgtcgta ccaggaatgg gggcagatat gcgtttgggc
      121 gatgtgatgc aacaagagca tgcagatatg ggtatctctt tttgtggtag tggtggtgca
      181 ggtgcattaa cagcagcaac aaaatatggc tatccagaac gacatggtat gcgttcgatt
      241 gaagaaggga tcacagctat caaggatggc aaaactgttc taggtttcgg ctttatggat
      301 caagaggaat tagggaaacg tctgacagaa gaatttatca aaaagcatgg aaaatagaaa
      361 gagggacatg aaatgagcgt tcaaaagaca attcagcaaa aagttatcgt ctccggtaaa
      421 ggggaaacaa agcaacatgc ttttgcatca gcgttagcag aaattcaaaa caaaataatg
      481 gaacaatcaa acgtattatt gcgaatcgaa ccagtcaatg tggatatttt aaaggcggtc
      541 gaaaacaaat acacagaacg ctttctattt ttcttcttcc cacggcaacg tattagctat
      601 gaagtcgaac tctctgttac tgtggctgta acagaaataa aattggcaga catatcgttc
      661 agccagcaaa tgactgaaga tccaaatggg attgccattc catttgtaac taaaaaaatc
      721 taattttgaa tcataagggg gaaagaataa cgtggatttc gtaatcattc tagtcaaatc
      781 ggtcattatc gggggattac ttggttttgc agcatctgtc ggggcagctc gaatgtttca
      841 tgcacctaat acacaaggtt taggagcttt tagaacatta ggcgaaatga atgcctgcat
      901 gggtgatcca gcttctcatt tttcatttgg tttaggcttt ttctttaatg catgggcttc
      961 ttcagttggt gctggtgcat tcacacaaga tgtgacgcat cgaatcattc ccaattgggc
     1021 agcggcagca cttctagcaa aaaacaaaga tgtcacccag accatgcatg atccgaaaaa
     1081 aatggggatc attggagcgg ttatcggggc agtggtggta gccttcttaa acacaacagc
     1141 tgcagcaatt ccagaggcgt tacaagtgac ggcggtttcc gttttggtac cagcagcgac
     1201 gattctgatc aatacagtaa tgccagttat tttttggttg gcagctttag acgcagggaa
     1261 acgaactggt ttttggggca ctctttttgg tggtatcgcg caattgatta tgggaaatgc
     1321 ggtacctggt gttgttctag gaatcttgat tggtaaaggc gtcgatgaga gcggttggaa
     1381 tcgcgtcacg aagagcatga tgatcgctat tgttttatta tttattttca gtggattttt
     1441 ccgtggattt gatatggaaa tgatcaagag cttccagctt caagtgccta attggcttga
     1501 taacttgcac aacatcttta gtgtgaaata ggaggagaat catggagaca acagaagaac
     1561 aagcaaaagt attagaagca aagcggcaaa agagcttttg gtttgcagac tggtcttttc
     1621 cgctaatcgt aggaattatg tccgctgctg tatttgcagg aacacacatg tatgtgatgt
     1681 atggtgtggg tgcattcaat gaagtttcga tcgtggcgat gttaaaagcc gggctggatg
     1741 gcggatccta tggcgcagcg gcagcgtttg gtgctagttt cttatttgcc agaattttag
     1801 aaggctcttt agttgggatc ttggacttag gcggttcgat cctaactggg atcggaattg
     1861 gggtaccagc aattctctta agtatgaaaa tagcggcacc aatcgataat tttgcactct
     1921 ctttattgac tggtttggtt ctaggtttga ttgttggtgg catcattgtc ttgattcgta
     1981 aatttacgat caatcaatca aattccactt ttggtgctga cgtcatgatg ggggcaggaa
     2041 attcatctgg acgttttttg ggtccattaa ttatattaag cgcgtgtgca gcatctattc
     2101 caatcggcat tggcgctaca ttaggagcac taggattcta tgcttggaaa aaaccaattg
     2161 ctggaggtgc tattttagga gcaatgattt taggagcaat cttcccggca gtaaattaat
     2221 agaaagaggg tcattaaatg tttgatactt tgattaaaaa tggaaggtta gttgatggat
     2281 ctaaaataga ggtcgctata gaaaaaggca tgattaaagc agttgcagca gaaataaatc
     2341 agccggcgca agaagtagtt gacttggagg gaaagtatta tttatccgct gggtggatcg
     2401 atgatcatgt ccactgctat gaaaagatga atctttatta tgactatcct gatcagatag
     2461 gggtcgaaaa aggtgtgacg acggtcattg atgctggaac tacgggagca gaaaacatcc
     2521 gtgaatttta tcaattgacg aaatcggtca agacaaatgt gtatgcgcta atgaatatct
     2581 ctaaatgggg cattgtcaaa caagatgaac ttgctgatct aacaaagatt caagaagatc
     2641 tagtaagtac tgcgttagca gaactgccgg aatttattgt cgggattaaa gcgagaatga
     2701 gtaagacagt tgttggtgaa aatggcatta agccattaga attagctaaa aaaatccaga
     2761 cagaaaacaa tgatctgcca ttgatggttc atatcggttc tgcacctcct gagttagaag
     2821 aagtattgtc aagaatggaa agaggcgatg tattgaccca ttgttttaat ggaaaaccaa
     2881 acggtatttt agatccaaag acagatcaaa ttaaaaactt tgtctgggat gcttacaata
     2941 atggtgtcgt ctttgatatt ggacatggga cggatagctt taatttccat gtggcagaaa
     3001 ccgcactaaa agcaggaatg aaagctacct ctattagtac ggatatctac atccgcaatc
     3061 gtacaaatgg cccagttttt gatctagcaa ctactttaga aaaattgagg gtagtcggct
     3121 attcttggga agaaatcatt gaaaaagtta cagcagttcc tgcacggaat tttcatttga
     3181 agaataaggg ccagttagct gtcaattatg atgcagattt gacgatattt gagattgttg
     3241 aaaatgaaaa ggaattgaaa gattccaacg gatttacacg tatagctaaa gaacaaattt
     3301 taccgattaa aacaattatt ggaggcgtca tatatgacaa ttagctatgg aaagtttgat
     3361 ttaaaagaag tcattaatgc atcgggaaaa atgacgatcc taggggtttc taaggtatct
     3421 gaaagtgtcc tagcagcaca acgatttgga ggcgagcatt tttttgagat gagtgatctt
     3481 gccatcaaaa ccggcgctta tctttctcaa ttactgaaag tggaagatgc gcaaatcgtt
     3541 tcttctgctt cagcagggat tgctcaatct gttgctgctt taatcggaca gggcgacatg
     3601 taccatgtct atcatcccta tacagaaaaa atcgctagac gggaaatcat catacctaag
     3661 ggacataatg tagattatgg gacaccggtt gaagtaatgg ttgctcaagg tggaggaaaa
     3721 gtcatagaag ctggctatgc aaatatgtgt acaccggaac atgtttccat gatgattacg
     3781 gaaaaaactg ctgcgcttct atatattaaa agtcatcatg cagttcagaa aagtatgctg
     3841 acggtggcag aaatggtaga agttgccaaa gctcatcgtt tgccattgat cgttgatgca
     3901 gctgcggaag aagatttatt caaatatagc gaaatgggcg ttgacttagt gatttactct
     3961 ggggcaaaag caatcgaagg tccgagtgcc ggactggtca taggaaaaaa gaagtatatt
     4021 caatggattc ggctacagtc taaaggtatt ggccgagcaa tgaaaatcgg taaagataat
     4081 attttaggct ttacccaggc actagaagat tatttaaaaa atgggagcga atccggtgac
     4141 tccatgaagc aacgattagc gccttttgtt aacgcaatca atcaacttcc tgacctttca
     4201 gcgaaaatag ttcaagatgg tgccggcaga gatatttatc gagcaagtgt aacgattaat
     4261 ggcgataaaa gtgcgaaaga agtcattcag gaattaaaag cacgaaatcc agcagtctac
     4321 acccgagaat atcaagcaaa taacggaatc attgagttcg acattcgttc cgtcagccaa
     4381 aacgaaatgg aaaaaattat aaaaagattg agagaaatca tgtaggaggc ttttaaattg
     4441 tcagttatcc caaattatta taaaaatcgt gtatgtttaa atgtattagc tcattcagtt
     4501 gaaaatgcaa aagcatgcta tgaagcagcg gaagggcatg tcgtactagg tgtgctatcg
     4561 aaaaattatg ctagcgatga agcagcaatc gaagatatga aaaggtacca agacgcaacc
     4621 aataatgcgt tgtcagttgg attaggcgct ggagatccaa accaaagtca gatggtttcg
     4681 cgtttatctg gggttttaca accacaacat gtcaatcaag tatttactgg cgtggggact
     4741 tctcgtgctt tattgggcca aaatgataca gtcatcaatg gattggtttc accaacaggt
     4801 aagatcggct atgtcaatat tgctacaggt ccgttaagtt cacaagcgcc agcagctgaa
     4861 gtgcctattg aaactgccat tcaaatgctg caagacatgg gaggtacttc aatcaagtat
     4921 ttcccgatga aaggcttagc acatagggaa gaatttaaag cagttgcgga agcttgcgca
     4981 aaatatgaat tctatttaga gccaacagga ggaattgatc tttcaaactt tgaagaaatt
     5041 ctgaaaattg caatcgatgc aggtgtaaaa aaagtgattc ctcatgttta tagttcaatt
     5101 atagataaag aaacaggaga tacgcgtata ggagacgtaa agatactatt aagcatggta
     5161 aaaaaaatta ttcactaact cattagaaaa taagaagctt attccagaat gaaattaaat
     5221 ggaataagct ttttataatt aattacggaa attgttaatg tagataataa tttagatgta
     5281 cgtaagcaga agtacagaaa tgtaagattt tccaatatta ttttattcaa cataataaat
     5341 cgtatatcaa tttttttagc gaaatttcta tttattccaa gaatttcagt tattacggaa
     5401 tcattttgtg cttaataaaa agaaaccgtt tttatatact ataaatgtag aaaggcggta
     5461 ggaaaatgaa aaaaattatt gcaatcacat cctgtccagt tggtattgcc catacataca
     5521 tggcagcaga aaacttagag aaagcgggga aagcagttgg cgccgaggta aaagtcgaaa
     5581 cacatggttc catcggtatt gaaaatgaat tgactgcaag agatatcgaa gaagcagctg
     5641 gtgtaatcat cgcagcagat actaaaatag ataaatcacg attcggcggt aaacctttga
     5701 taaccgtagg tgtgcaagaa ggaatccacc atgcagatga gttggtgaga gatattttag
     5761 caggaaaagc gccagtctat aaaagtacgg aggcaattat ttcttccgaa aataaaagtg
     5821 aatcagaaaa tttaggtaaa aaaatctata agagtttgat gaatggcgtt tcgtacatgg
     5881 tacctttcgt agttacagga gggttattga tcgcgatttc tttaacatta ggaggaacag
     5941 ctacacctga agggatcaag ataccagaag gaacgatttg ggcaacgatg aatagtatcg
     6001 gaagtatcgc tatggggttg atggttccaa ttctatctgc atttattgcg caaagtattg
     6061 cagatcgtcc aggacttgtt cctggatttg ttggtgggat gttagcggca aacggagcat
     6121 tatatggtag tgatgcgaat gcgggattct taggcgggat catcactgga tttttagcag
     6181 gattcattgc tttaggaatc aagaaagtgc gagtaccgaa agctttacaa tcgatcatgc
     6241 caattattgt tatcccaatt ttaggatcat tggcagttgg ttttgtgttt atctatgtta
     6301 ttggagaacc agtagctctt ctatttagtt cattgactca ctttttagca gggatgcaag
     6361 gaggaagtga aattgttcta gctatgatct taggagcgat gattgctttt gatatggggg
     6421 ggccgttcaa taaagtagcc tttttgttcg gatctggctt gattgcagaa ggcaattatt
     6481 ccattatggg gccaattgct gtagctatct gtatccctcc attagcattg gggctggcat
     6541 cattgatttt aaaaaccaaa tttacgaaaa cagaacgaga agctggtaag gcgtccttag
     6601 caatgggact gtttgggatt acagaaggcg ctattccgtt tgcttcaaca gatccactac
     6661 gggtgattcc aagcattgta atcggggcaa tgacaggaag tgtgatcgcg atgctttcag
     6721 gagtgacaga tcatgtagct catggcggtc cgatcgtagc ggttttagga gcagtggatc
     6781 acgtagccat gttttttgta gcagtgattg ctggtattgc tgtaaccgtg ctaatgttaa
     6841 gaatccttaa accgacatta caacaaactg cggttgctgc agcaaatggt gtgacagtag
     6901 cagatctatc agagactgaa acaagtgatg tttatacgaa aactgaaact tttgattcta
     6961 tcacggatat tatgtcagat gaagcaatcg ttttaggtga aacagcaaca acaaaagacg
     7021 gagtaatcga agatttagtc caacgtttag aaaaaacagc ggcgatcaca gataaaacca
     7081 gatttaaaga agtcatctat gatagagaac atgaaagtac aacgggaatc ggtatgggga
     7141 ttgctattcc acatggaaaa tcagcttatg ttcttaaacc aacagtggca tttgccagaa
     7201 gtcaatctgg ggtagattgg cattctttag atggaaaacc tgcacatatg atctttatga
     7261 ttgctgtacc tgaaaacagc caaggtgata tgcacttgaa aatactgcaa cgtctatcac
     7321 gtaaattgat ggatgatgac ttccgtcaag cactgatgga agcgccagat aaaacaacag
     7381 ttcaccaatt attaagcgaa atgtaacata agatgaaaaa tcgaccgtga tgagacataa
     7441 tagatgtgtt atcatgatcg attttttatt tagaagggag tttctgcaca ttatgaatca
     7501 gcgtcagcaa tctttgatct atcaattaag tgagaacaat aattatataa caacaaaaaa
     7561 gctagcagag catttccatg tgtcagaaag aacgattcat agcgatttga ataccattga
     7621 aacttggttg agagacaaac atctgccttt aatgtttgaa agaaaaaaag gtacaggtat
     7681 cttgttgaat ggtctgcctc acgaaaggga acaactgaaa cgagcgctta atgaagagac
     7741 tcaaacagaa atggataaaa aacagcttcg tcagttattg attttccatc tgctagtagc
     7801 aaaagacgga tttagtcttg aagaactttc agaaaaacta tatgttggaa aacgtacgat
     7861 tcgtaaagaa ttgacagagt tgcaaagctt ttttgagtat caccatttac agctagtatc
     7921 caaaacaaaa ctaggaactt ttcttaaagg cgatgaacaa gaaaaaagac agttgcttgt
     7981 taagacatta cgaaatatgc agaaagaaga tccacaaagt ccgtcgttga aagaattctt
     8041 cgctaaagat acattaaaag ttatccaaca tacattacaa gatgtttttt atgaaaacca
     8101 tttagaacag ccgaatggac tggctagtgt ggagattcac atctacttta tgctcgagcg
     8161 aatgaaacag taccagaaag tgaaattaag taaagacgaa aacgaagtcg tggaacacac
     8221 ccaagcacaa aagttaagta gtcaaatatt agcaaaacta gccaccatct atccaattga
     8281 attttctcct gatgaaatca attatctggc tttgcgtatc gccaatcttt ttacaaattc
     8341 acaagcagcc acacgttttc aaaaggaatc tagcgcactt gctgatcatt tgatcgaaca
     8401 agtggaacag tttctaggct attcattaaa agaagatcag ctactaaaac aaaatttaag
     8461 gtctcattta tcttcaactt attttcgact ccattatggt ttgcagattt ctaatccatt
     8521 gacgaaaaat gtgtttagta cgtacacaca acttttctta gttttacagt taattttaga
     8581 agattatttt gaaaaggaaa atttttatgt tccacaagag gagacagctt atttaacgat
     8641 ccatttccaa gcagcgatcg agcgacacaa acatcagaaa agtagaagat atcaaacggt
     8701 tttagttagt gaatattcaa aagctatggc tacttttctc gaagcacggc tgaacagaga
     8761 acttccagaa ttaacgatta tggatctagt tgaatataag ccagaattaa agagacaaga
     8821 ttttccctct gttgatttta tcttggctac gttaccattt aaacatataa gcattccagt
     8881 aatcgagatc tcaccaatga tcacagaaac tgatctagcc tatttgacaa aatatatgct
     8941 ggagcatgtg cctatcaaga agaaaaaaac atttgatcta gcaagcttta ctcatccatt
     9001 tctgatcttt cctcaacttg aacggacaga cccagccgat attttaaact ttatgggaaa
     9061 cgtcttagtg gaacatcatt atgtggagcc ggaattcatc gattctgtct tggaaagaga
     9121 cagtcactcc agtactagag tagcaccatt tgtaacaatc cctcacggta atccgctgta
     9181 tgtgaagcat tcgatgattt ctattgctac aatgaaagaa ccagtcctat ggcacgggga
     9241 acagatacgt atcgtactgc tattagcaat aaagaaaaat gatttgaaac atgcagagtt
     9301 caaaaaaata ttttctgtta ttcactatct agaaaagcag ccagaacaaa tggctcagtt
     9361 gatgcaatcc aatcatccgc tagaaatctt aacgttgtta tctagttatg aataagtagc
     9421 gattaaaaaa ctgcctttat atttataata attctatcta tttatttgga agtagaaaac
     9481 taaatacaaa aaaagaggat gtggccaaag ttgtggcaca tccttttatt ttgagtttct
     9541 tataattttt tatagttgct tacgatgtct tgactactgg taaacggtgt tcaagagaca
     9601 atttgtcggc aataaggcac aattttgtaa aatatggaaa gctatttttt taacattgtt
     9661 tcttagtgct tgaaaaatca ttttgaatac ttctttatac gatcgatgta cgttgattta
     9721 agtctaaaac ttttcttttg attccttctc tgacttttct gaattccgtt aatatttctt
     9781 cttcgctccc gacagcttgt gcaggatctt ttaggtccca gtgaaggtgt tcgtgattgt
     9841 ttggaacggc agggcacttg tctttggcat caccacaaag tgtaatgatc aagtctgcct
     9901 gagtaaagta atctctatca atcaaatca
//
LOCUS       DACDRN010000086         9637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDRN010000086 DACDRN010000000
VERSION     DACDRN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.086"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(162..3803)
                     /locus_tag="IWJ80_002441"
     CDS             complement(162..3803)
                     /locus_tag="IWJ80_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462127.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVVYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMIDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGQDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEDDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTALKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKPSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            complement(3806..5755)
                     /locus_tag="IWJ80_002442"
     CDS             complement(3806..5755)
                     /locus_tag="IWJ80_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9462128.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(5797..6855)
                     /locus_tag="IWJ80_002443"
     CDS             complement(5797..6855)
                     /locus_tag="IWJ80_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP9462129.1"
                     /translation="MKKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(6974..7429)
                     /locus_tag="IWJ80_002444"
     CDS             complement(6974..7429)
                     /locus_tag="IWJ80_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462130.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(8012..8815)
                     /locus_tag="IWJ80_002445"
     CDS             complement(8012..8815)
                     /locus_tag="IWJ80_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9462131.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            9462..>9637
                     /locus_tag="IWJ80_002446"
     CDS             9462..>9637
                     /locus_tag="IWJ80_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9462132.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWG"
BASE COUNT         2826 a         1974 c         1387 g         3450 t
ORIGIN      
        1 taccagcata agcccttttt aattatacaa ctttgaacag taaacattta attcatttat
       61 agacaattta aaataatttt tttgcataaa tagaaatttt ccttgacatc ttcagttaga
      121 aatcaaagta aaaagaagga gcaaatcaat gctccttctt tttactcgtc ttcagactta
      181 ttttcagttg atttttgatt accgtatttt acaatatagt ccttgactga catggtagat
      241 ttcttcacta ctttaccagt tttggatgct cctgtagcag tagccttggc aaccttagcc
      301 gttcctttac ctattttaat agcttcgttt ttagcttcat aggttatccc tgctagacta
      361 ccttttggag cttttgctaa agctttatct gctgaggtgt aaacattttt accagttttt
      421 gatagatatt caacggctga tttagctgtt gatggtttct tggttgattg attagatttc
      481 cctgaattat ctgaagtaga tttagaatta tttgttctat tatttttatt tgtcagttca
      541 ggttctctaa ttgattgatt gtctttcgac attcctgatc caatatttgt ttcatattta
      601 tctgtttgat taacattagg agtaggaatg aactttttct tcacatctga ttgttcttta
      661 tccgtttctt ttgttagcga ttctctgtct ttaagtgctg tgtcattccg aatagcacta
      721 ttatcaaagt tgctgttaag ctctcttgac tgactttcac cagatgctcc atctcctaat
      781 tcccgatgca ctctgtctga cacgtcctca ccactcattt cattcctcag ttcttgatga
      841 gtttgatcta aagtaatctc gtcatcttca ttctcaggaa ttgcaacagc aattggcgta
      901 tgccgttgtt gttcgagttc tctatttcga tctgccacaa gcgcatctct cgtctcaatg
      961 atattcccat cctcatcgac taaatctcca ttcacattat atccatcgct gtgtttacca
     1021 agtttattac caaactcatc aacaagattt ccatcttcat caactaaacg acctttttcg
     1081 tcaactaaac gtccgttact atcctgacca aaatcaccat ttagcaacct gcgtccattt
     1141 tcgtcaacaa gatttccatc ttcatcgact aagcgacctt tttcatcaat taaacgtcct
     1201 tcgctatcca taccaatatt accatcttca ctatcttcca atggtttacc attagtatct
     1261 ataaggtttc catcttcatc gaccaaacgc cctttttcgt caataaagtt gcctttagca
     1321 tctttaccgt atttactatc ttttaatatt tcaccatttt catcgacaaa gtttccgttt
     1381 tcatcaatca tacgtcctaa atgatcaata tattgttggg agttattacg tagttgatct
     1441 tgacgaacta aatctttgaa agaggtatta ttgattgttg aaccaactcc gccaccgttg
     1501 aatgacattc cttctggtgt ttcaggaatt actttatcag caatggactt agcatcagca
     1561 ttgctatctc ccatattgtt caaacgttca gccatttttt gcgctccaac tgctccaaga
     1621 cctcctgcaa ccatatcttt tactgagctg ttgccaccat cataataggg atttttagct
     1681 ccttggcttt tcttgattgc tttagaaacg ccgtccacaa ctcgcacagc atcatttcta
     1741 ttaccaaata ataacttagc acgttcatca aacccaaacg ctacttcaag tggtaagcca
     1801 ataagcatat taatcaaatt atttactttt tcgccttttt taaagactaa aagatatcct
     1861 acacctaaaa tcatagcaga tcgaacaata tttgctacgc catcgccaac taatgtatta
     1921 ggaatgaatc ccacagcagt atcaccaaat gcacgaataa aagtaatcag aaagataggt
     1981 aattgtagtc cgatcagaga tgttgtaaca acacctaatg aaattgccat cacacctgct
     2041 cctgatgcaa gtgatcctgt tagaactact tcccaaatcc gaataaattc acgtttaaat
     2101 gctttcacga tattcacatt gaacatcgcc caagcacatg ctaagtaaag aactgctaaa
     2161 acgattgatt cagcgttcat cgctagccac gagccaaact tacccatcgc tccatgacca
     2221 actaaggatg ctgtacgcca ttctgttttt actgttgaca tattcttagc actgccttga
     2281 attcctgaag gagcgatatt atacgcataa aacttagctc ctgatgtatc aagtgctgat
     2341 tggagcaata aagctaccga ctgtgtgcta aaagttgcat tgttagcaac gccccaaggt
     2401 tgagtagcat caaagttatg cgctgttcct tttccatcag gcttgcgatt gtcgtctgtc
     2461 acattttgat ttgctgacca aatatatcca tttaaattgg ttggtttctt aattgcactt
     2521 ccttcttttc catcccctaa attatagcct gtccaaacag caggcgctct tcccatataa
     2581 gcatcatttt cggcgacatt aacatcaccg gcataagtat taatattgaa agtatcattc
     2641 gatgaccatt cttttaatag agccataccg actgaattag aatctagatc attattttgt
     2701 ctggcataac tgctagtatt gatcgctctt atcattgcag attttttaga tggttcataa
     2761 tctgtatcaa cgaacttgat ttcggatgta ggctttccgt caggtacatc accaccagta
     2821 tcagatgatg gagcaagatt ttgagtagaa gcccaaagtc ttgtgttaat gatgtagtca
     2881 gaaacaacat ttgttggtat atctccatct ttttttgctg actggatgcc gttgatataa
     2941 actcccaata gcgatcctgc cactggtaag aatataaaca atacaaaaat acgaacaatc
     3001 catttttcaa gtactggtga taatcccttc gagttaacac catctttcga caataatttc
     3061 attccttgat aacctagtat tgctgtgaaa aagagcatag ataagcttgc tatcgcttta
     3121 aataattcat tatttaatcc caaattgtta agcaagctag ttaatgcttt agataccgga
     3181 ttagatacat ccgatccacc ttctccccac cccataagag aaaatatatt catactcatg
     3241 aaaaattttc caaggagttc atttatggat tcagcaatat tagaaaggat taaactaatt
     3301 gatccgataa aactaccaat cgtcctacca atacttccta gaatggatga tgcttgactg
     3361 gcaatagcat ttcctgagac tgtttcaata tatccgtagt aactataagt tgctaaagta
     3421 gatgctagtt gagtacctgc attaccccct gcaatcgaat taccttgttt agaaatgtca
     3481 gaataaggga aatcggcact gataccacct tgtcctaaca aattcatttg tccagtttta
     3541 gcggcactat cattaacttc tttatctccc attaggatac gagcatatag tgtccacatt
     3601 ccttgttctg ccggtttact ttccgcatat acaacgttct tatttataac tgtttcttgt
     3661 ttgctatctt gattgtcagg agttggatca ttcattccaa aaggagcgcc taaagtaact
     3721 gctaatactc ctccaataac tagacctctt acgacccatc gttttattct aaggttttgt
     3781 agtaaatatt tcttgaattt catagctacg cccctccaat cgaaaataca atcttgtttt
     3841 ctttactttc agcaacggaa ggctttgcaa taatttcagc attagaatca ccttttcctt
     3901 caaacgctcc ggcttcactc aacttaacat cgaatatttt tgcgtttgga taagtaatta
     3961 ttgcttcagt aatcgcttct tcaaactcag attcaactaa atgcaagata ttccttgcac
     4021 cccctgattt agaatcctct tgtgaagcca tgtcaaaaac taagtgcatg gagagatcgt
     4081 ctgcataaaa attttgacca tatactcttt ctttcctagg tggaagacgt aaatctatat
     4141 ttaaatgttt tcgtactaat tcagcactct tcaacatttc attataagca atttccagaa
     4201 gagtttgttc ggataaagcc caaaatggaa ctattcgtcc aaatcggtta ataacttcag
     4261 gctttagacc ttcaccaatc aaggcttgtt tgatccctga tgcgactttt cggtattctc
     4321 tttttagttc agtgctatat tcattagaat tttccttgtt tgtaacacga gatagtgagc
     4381 tatatctgtt taacgaatcg aacatctccg cacctgcatt tgttgtcgca aataaaattg
     4441 tatttgacaa tgaaacggtg tcgtgaactt ctcgatcatc ccatcctctt tcagtaaaac
     4501 ttatcctacc ttcatctaaa attgataaaa aggcatcgag tgtttctaca ccagctttct
     4561 catattcatc acacacaata atcgcagaag gatcatgcct tacatgagta cctatgagtc
     4621 gtctaaaacg cattgcatca tcttttgttt tgtagtccgg catattaact aagagaactt
     4681 tattttcatc gccataaagt cctcttgcca cggctttagc cattgcagtt ttaccgaccc
     4741 ctgttgatcc tgtaaacaga gcagttgctt ttggttgctt tcgaccttta aaacgatcaa
     4801 atccaagctt catacgttta aacaacattg ttatagtata aactgctaac ggttgccctt
     4861 ttatggactt taattcctct tgaagttttt ccatatcaaa atcagaatct agctgaatat
     4921 tatacttacc aaatatttgc aatagacttt ctcgattata agcatttccg tctactcgat
     4981 cttttgcaat caaatcctcc attacggcaa ttgatttagc cggttcggac aaatcctcac
     5041 gataaagctt gtttgcttgt acgatttctt gaagtaattc atcaggaatc ctttgcgata
     5101 atttaaaaga agcctgagga tcatctttaa cagaggatgt atttagccat tgtctcaaaa
     5161 tatcgacgac aacatcactt tttagttcat tcaattctaa cggttgtaac cgtctcccta
     5221 aggcatcatc tcttttgata tagttttcat attcttttct tgtagtagcg gtaatcaatt
     5281 gaagtgattc atttgctaac ggttctttta gcgaatcacc cccaatttta ttgccactac
     5341 caaaagagct aacaacttta tgaaactcgt caatgaagag gacaaattca ttttctttgt
     5401 cctcagattt taaaattcgc tcatattctt tcaatcttgg aattaatgtt tctaaacgtg
     5461 tttggaggac atcatctccc ccaacacgca attgtccgat tgataatttg tatacttgta
     5521 ctttctttcc atcttgtcgc tgtctttcag cccattcact tgcaagtgct gttttacctg
     5581 ttccggcttc acctaacaag atagcttgcg gtgtttccgg tctattcaag atcagtgaca
     5641 actcatggag ttcgtcatca cgtcccacta acttatgttt cggtggtttc attggtgtga
     5701 gagcatgatt aaattcagtt tgaatattct taaaaaactc ggtactattt tccatctgtt
     5761 tgtgttcact tcctctaaaa taagaataat ttacttctag tcagtcagag ttaggtactt
     5821 aaatgtcgtc ttcatgtctc caaaagaatc tttgttaaag tcagcatttt tgtattgatc
     5881 aatcaatttt tgtgtatcat tgattgcttt aatttcggtc tccattcgac tgtctagttg
     5941 acgaatactt tcttgcgaat ctttatcgtc aggattttgt tgtaaacgtt ccagatactc
     6001 agcttttgta gcttcaattt tcttgatgtt agtatttgaa gcatttaact gatcttctag
     6061 cttatttaca acattattct tacttgtaac tgctacaact tttgaataat caatatcgcc
     6121 atctttggta agaaagcttc cttgatagac attagttgaa tctgaatatg gatttattct
     6181 gaaattgata ttatcaggag tattaacagg ttttgctttt ttcccaaaag aaattactcc
     6241 ttcattatta acatcattca tagaatattg agaaagtgct tttgtaactg aatctgcttc
     6301 attcacattg actttggtat catcatcagc tgtatcatca ccagaaatta gtgatacttg
     6361 gtttgcaata aaaacttgat aagctctttc atctaattta ccttttagga ataagaaagc
     6421 atccccatca gtacttaaca ttccatagct catttcaagc ttcttaggac gatctttttc
     6481 ttttgttgcc atatataagt tgtagttttt tccttgcgta gataacaact ttctagcgtt
     6541 ttgcgagtaa ccgagtttta ctactgttac atctctgttt tgatctgtcc acacatctga
     6601 aatagtgatc tctgctcctg atcgagcaaa ctccaatgaa gagcctatag gtgttgtatt
     6661 gttcacgaga taataattat agtttcctaa ttgataaatg tagctaccca agcctgttaa
     6721 taaaaaaagc actcctagaa aagttgcaag cataatgcgt aatccgtgac gtgttctctt
     6781 atccttatca ccaagcaacc ctccaataat actgtattga tctttggtag atgttgaatt
     6841 gacgtttttt ttcattagtt tgtccgcccc ctttcataaa agagcaaaca atcagacgtt
     6901 ttttcggtaa ttctaggttc gggattttta atcatttcgg tatattcttc cccattaata
     6961 tttaacacaa aaattatctt cctttcttcg ttcctttctt ttgctcttta ttttccaaat
     7021 aatcaatgat ttttgctatt gtaccaagat agtaattccc ctcggctttg ttttttcgga
     7081 ttttgctaac cattggagta ggtacaatct cttcatctct caattcactg aaagattttt
     7141 tacctagata ttttccaata acagatttta aaaaacgttc aaaatctttc tcgtaagttg
     7201 ttttcttgcc ttttagttca tggttcatat tgtcaacgac ttttgcggtt tcaataattg
     7261 ttttagttct cagcttaata atttcatctg gaattttcaa tcctcgtaat ttcatcgttg
     7321 tactaactgg taacccatgc tttactgcat cagcaatact aaaacttttt agatcatcta
     7381 gtaagctctc catatagtta acaaattcat gccaattaat agcattcatt gtctcacccc
     7441 ttcatttttt taaaatgagg ttcgggcgta acacatttat gttattataa tatcataata
     7501 acataaataa atcaatcagc aaatttagat atcccttaaa gtatcagcaa ttattcaaca
     7561 agatatttat tttagctatt tcagtcatca tgaaagacta attagcaata aaataactaa
     7621 tattatccat ttatcagacg aaaagaaaaa acttatctaa ttactctata ttaactttta
     7681 acaaatagtt gacttgtcca aaaatgttac cttcaatctc acaaagatat taagatgttt
     7741 cctaccaaaa ctgatatcac cggacttttt attctgtgtt gtccaaaagt gttaccttta
     7801 gtcgatcaac aaattaaaaa aattgttttt ttcttgactt gtccaaaagt gttacccata
     7861 gttgttaaaa tttgtataga ttagccattc tttcaaatat tatttagact gaaacttgct
     7921 tacaaaaaaa cttcttgcat ttattgtcca aaagtgttac ccatactttt ttaaagaagc
     7981 aggttgtatt aaaattacaa cctgcttctt ctcaatcttc ttctaaccaa ttatgcattg
     8041 aaatggttgg caagtttgat tctggctcac taaaagtgaa tctgagccga tcaactcttc
     8101 ttcctttctt tgcatagatt ttttcataat caaaagtttc aaatacctgt attccgtttt
     8161 catcaggagc taaaaattct tctttcgcct gctctagtat tcttttgtca atatttgtca
     8221 ttctataact tgaaggtata cttaatgctc ttctaaaatc ttcaactttg actacccaat
     8281 aaccatttcg ccactcttta tttctgtatt gcatgagatg acgatacaat tcttttgtgt
     8341 agctacttct tagttttgta aaattatcaa gttcaaaacg agtaaaattt ttgccaattg
     8401 agtttaagat aaattcaaat tcctcatttg ccttaattgt aactgtttcc ttctgtttat
     8461 ctatccggaa tttttgaaaa agccacagct gttcatagac gtctgaatct tcaaaacggt
     8521 aggttaaagt acctagtttg tcactcaaag cttttaatac agaataaaaa ctgtcctttt
     8581 ctcttctgtc gtatttgacc aaataacgaa agaggttgaa atcaaaagtt acttctgctg
     8641 ttccttgacg tttcattttt gataaaatag cccaaaacac atccatttca acaggcttaa
     8701 atcctcgcaa agctgttgta ttcagaccat tgttataacg cacaattgca tttgtttgtt
     8761 ctgatactaa atctacatca ctatcaataa tcttttcggt aacttcttga gacattgatt
     8821 actctccaat cttctttttg cgctccttta atcatgttaa tctggtacta attaaaggaa
     8881 acattttttt ctgcttgtta cctaaattaa atcacaacta aaggtaacag tcaacaaaga
     8941 tttgttacta atctaatttt ctattttttc gccttccaaa tcaagaaaac cgaaggtaac
     9001 gtcttatcct agtatgatac ctcaaatgtc cacttttgtt acctttgctg tccaaaagtg
     9061 ttacccttac tgtccacttt tgttaccttt actgtccaaa agtgttacct ttatacccct
     9121 ttaatactta ctctcccaag agattaagcc tcccctaaca gatatattaa cagatattta
     9181 acagatatat taacagatat ttaataaagg aggattactc ctctcttatt tcaaaatctt
     9241 aaaaaaaaac taacactatc taggagttta ttttgcttgt gtaactttga ataattgaaa
     9301 aaaaggaaaa ggaggaagct tcagcttccc cttttattta tatttataat acccgtggtg
     9361 ctagttaatt ttttcctaga ggaaaatgtc taaacatgtt ggtaaatcaa caaaaaaacg
     9421 catatagtag tctcaaaact cccaagaaat gaggactaca tatgcgttca tcaaaatata
     9481 ctgaacatta tacagatact tttataacat tcaaggaaat tatgagaact gtttctaaca
     9541 tataccacaa ttgtgtacca gataagatta aaaaccgaag aaatactgat caactgaagc
     9601 aacgcgatac agtgattatt gcttgtgtta tatgggg
//
LOCUS       DACDRN010000087         9553 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDRN010000087 DACDRN010000000
VERSION     DACDRN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.087"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            168..362
                     /locus_tag="IWJ80_002447"
     CDS             168..362
                     /locus_tag="IWJ80_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462133.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IWJ80_002448"
     CDS             631..930
                     /locus_tag="IWJ80_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462134.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IWJ80_002449"
     CDS             complement(1027..1461)
                     /locus_tag="IWJ80_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9462135.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IWJ80_002450"
     CDS             complement(1693..2403)
                     /locus_tag="IWJ80_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9462136.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IWJ80_002451"
     CDS             complement(2393..3253)
                     /locus_tag="IWJ80_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP9462137.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IWJ80_002452"
     CDS             complement(3258..3896)
                     /locus_tag="IWJ80_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP9462138.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IWJ80_002453"
     CDS             complement(3911..4210)
                     /locus_tag="IWJ80_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9462139.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IWJ80_002454"
     CDS             complement(4253..5737)
                     /locus_tag="IWJ80_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9462140.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IWJ80_002455"
     CDS             complement(5757..6218)
                     /locus_tag="IWJ80_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9462141.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IWJ80_002456"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IWJ80_002456"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP9462142.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IWJ80_002457"
     CDS             7521..8291
                     /locus_tag="IWJ80_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9462143.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IWJ80_002458"
     CDS             complement(8564..9391)
                     /locus_tag="IWJ80_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9462144.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2981 a         1948 c         1491 g         3133 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgcccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatacttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg tta
//
LOCUS       DACDRN010000088         9085 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDRN010000088 DACDRN010000000
VERSION     DACDRN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.088"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..281)
                     /locus_tag="IWJ80_002459"
     CDS             complement(<1..281)
                     /locus_tag="IWJ80_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9462145.1"
                     /translation="MSITKNIKCTDEKTSHSIKQILNIKDSHITFSKDAISKERVHGR
                     MANVFTGLLTGEAHRCECCGFDSVIRHAYHDSWIQLLPYQEVPTYLHLY"
     gene            complement(429..987)
                     /locus_tag="IWJ80_002460"
                     /pseudo
     CDS             complement(429..987)
                     /locus_tag="IWJ80_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302360.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1156..3267
                     /locus_tag="IWJ80_002461"
     CDS             1156..3267
                     /locus_tag="IWJ80_002461"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012579.1,HMM:NF013070.1,HMM:NF019826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9462146.1"
                     /translation="MNTKDLKLLETIVRSHVTNSQQLKSLVHFSTRQITYSIKKINDE
                     LSLHGLSLIKRSNSGEWLYEANDLQKLLIYMNQAMVFKEGAIPEYINDDLAYSEIRRY
                     SELLFILANPKKVTTQEIMAFIGISKNTARNDFSKIKKELPPDYTLIFDPQNGYMIES
                     SMAKLHTLLLEVFNYLFERHATVNRFESLFSEEREFVGFFIHTYEQTFKLKFSDNSLT
                     KLYYLIGLSLSSFKRRTKGQIDFSYDLQELDEFQMIRSCIDQHYQHAEIAIDYEWLTL
                     LFLSFNTISNEFEILDSRIYQVIMEMITLFQSKSAIRISNKEELTNRLMNHLRPAIYR
                     IRYELPFEIDDLAEIVKENHEVSAVLNFVKESIVPLEAFLRKTIPETELYLISVYFCS
                     ELLNSSIIETPVRKKAIVVCSNGLIMANVMAKTLKEIFPDIQFVASSSVREMDKFVED
                     VDLIFSNQPLKSEIPLYIIQPIMNEEEKNRLRNRVLIDFSTNNISKETRNLTEIIKRY
                     SAVQNEESLQTDIERFLINYANSLIYLDRQSKQAQKISFVELLVSEGIILVSTKQLTW
                     QNAMELSCQPLLKEQVINQNYQTILMSQMADEEAYYFLNNRIAIPHSSPENGVIKGGC
                     SLLISVDPIIFPGNRPIHFISPFAIGKKENYLLAINQLLQLSKDEEYQQEILLCTTED
                     EVRKKIKNFLRRKQYVNNEFS"
     gene            3245..3895
                     /locus_tag="IWJ80_002462"
     CDS             3245..3895
                     /locus_tag="IWJ80_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9462147.1"
                     /translation="MSITNFPELTIILRGYSFEEAMFVMEELAAMNTSVGIEVTTNND
                     DFARIITEGNKKFGQQLLIGAGTVLTFSQADQAIAGGAKFLLGPKKFSTPIFSLAKER
                     GVVTVPSAFTPSEIQVMFDEGADIVKVFPAVTVGPTYFKQLAGPFGIRPLMAVGGVTI
                     DNIAAFKKAGVVYFGVGSSMFQKADIQNRNGQAIRKSIQSYLDKLAEAGSEEDESH"
     gene            3882..4334
                     /locus_tag="IWJ80_002463"
     CDS             3882..4334
                     /locus_tag="IWJ80_002463"
                     /inference="COORDINATES: protein motif:HMM:NF012579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9462148.1"
                     /translation="MSLIKLEEKNILLNQSFSNQEEYFQKITKKLMTENMVKEGYLES
                     ITKRETTYPTGLYIGERSIAIPHTDYQLSNVTAILVSVLKDSLHFQRMDDPEEMVSVN
                     VIIHLLFDSPDKQPQLLKELMSKVTNQDFLQAIMEAKSKTEVLTAFNQ"
     gene            4351..4656
                     /locus_tag="IWJ80_002464"
     CDS             4351..4656
                     /locus_tag="IWJ80_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIB"
                     /protein_id="HAP9462149.1"
                     /translation="MSKKTIIVACASSMITSTMAANKIRKIAKTNGLPEPRILQLKFS
                     EVAGALESNQVDFIVPTGKLQENVTKGIKVIPGTPFVTGVNEEKSESLVLEALKELD"
     gene            4668..5945
                     /locus_tag="IWJ80_002465"
     CDS             4668..5945
                     /locus_tag="IWJ80_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010492829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IIABC"
                     /protein_id="HAP9462150.1"
                     /translation="MNIVIDALNWISSLGPMIMMPIIILILGLFFRIKLNTLLKSALT
                     VGIGFAGVNIIINWFISQVGPSVQLMVKNWGIQTSIMDVGWPARAAATWSFPLAAVMV
                     FIILGINVLMLIFKKTNTVMVDFWSYNHYIFVAALVYYATDKNIILAVIFGAIDAIIS
                     FKLADWTTPLVEDYFELEGVNFPTANSVGWAPLAWLLNKLWDRIPGLNKVDARPEKIQ
                     ERFGFVGEPMFMGFVIGLVIGLLAKEPVDKVLIVGMSTSATMVLTPKMMQILMEGLLP
                     FANSVKKFLNVKFPGNNFTMGVDAALTVANSSAIATGIIMVPITLVLAAILPGNKLLP
                     ISDIAYQAMWLSAWPVAFSKGNIIRGILSTTIITACVLLIATSLAGIHTQLALAGGFQ
                     LPAGMTQISTEDAGTHIIGFILMKIAEFISNIF"
     gene            5964..7112
                     /gene="dgoD"
                     /locus_tag="IWJ80_002466"
     CDS             5964..7112
                     /gene="dgoD"
                     /locus_tag="IWJ80_002466"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAP9462151.1"
                     /translation="MKISKITVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRFLVGQNPFAIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKALNVPV
                     YELLGGAARDKIKVYSWIGGDRPSEVVEQAQDRVDRGFTAVKMNATEELHYIDSYEKV
                     AEVVERVASIRERFGDTLSIGVDFHGRVHKPMAKVLAKALEPYHPMFLEEVVLPENEE
                     HFSEVANAVAVPLATGERLYTRWQFKSILQQGAIDIIQPDVALCGGILETRKIAAMAE
                     AYDIAVAPHAPYGPIALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEIFQ
                     YKDGFVDLPTKPGLGLEMDEDRVKEIAQEGLVWTNPKWKNYDGTIAEW"
     gene            7124..8026
                     /locus_tag="IWJ80_002467"
     CDS             7124..8026
                     /locus_tag="IWJ80_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryldialkylphosphatase"
                     /protein_id="HAP9462152.1"
                     /translation="MINTVTGPIEAESLGTTFIHEHLYVIPNELRKYEDYTLDDIYHS
                     IAEVMAFKQAGGKTIVDLTPINYGRNPLALKMISEATGVNVAFVTGFHKEEFQPKWLT
                     TLNDDEIFELLYSEITHGVTSQNLLPGAMKVGTSKNEVTLAEQRILAIAGQVQKKTGI
                     PIITHCDGGTMGLEQINCLTKNGAKASNICLSHVDLTKDFHYLAQICETGASICMDHI
                     GRELANHDADRVQLIKRLVDAGYENQICLSGDMGRKKYLPTYGGTPGLTYILTSLKEE
                     LLKVMSEPTFNKMIEMNPINILSK"
     gene            complement(8695..>9085)
                     /locus_tag="IWJ80_002468"
     CDS             complement(8695..>9085)
                     /locus_tag="IWJ80_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9462153.1"
                     /translation="FKQFPLFQGKYMTESEVLDYLLSIDNQLRQSYEVYQELLAAFDA
                     KDFTEFFNLIESLPHSLNDGFKKAICYLKKHTEAITNSLKYPYSNGKLEGKNNLIKVI
                     KRIAFGFRTFRHLRMRVLIQQNICEII"
BASE COUNT         2948 a         1397 c         1855 g         2885 t
ORIGIN      
        1 ttgtacaggt gtagataagt tggtacttct tgatacggaa gcaactgaat ccaactatca
       61 tgataagcat gacggatgac agaatcaaac ccacaacatt cgcaacgatg cgcttctcca
      121 gttaataagc cggtaaatac attggccata cggccatgaa cgcgctcttt actaatggca
      181 tcttttgaaa aggtaatatg ggaatctttg atgttcaaaa tttgtttgat ggaatgactt
      241 gtcttttcat ccgtacattt gatattctta gttatggaca tttggttatc ctcctatgat
      301 tgtttttgcc gatttaatta taggacaacc agatgtcttt ttgttataaa aaaatatttg
      361 gcgtcactac tcagtttgag taacaaaacc aaatattata gaaccggctt tttcagtttt
      421 ttcccttcac aaacaacatc gatgattttc tcggcatggg tacgggtatc attcattaat
      481 tctgtatcgg ttggatattt gatgttttgt ggagcacaag tggcatccaa aatcaatgtt
      541 ccggaattga aatcgtcata agaatcgttg tccttatcgt cttttttctt gtcgaagacg
      601 agtatttttt cattgatttc tatcaacgtt gtttcgtcca agcgtttccg gaaatagacc
      661 attgtagagc atcgaaaggc ttggcatctt gatagcccgg caaaccgatg aagaactata
      721 aatacgggtt ttcttgaatt tgtaagaccg tttcttcgtc gctatagcta tattcctttt
      781 gaatcagtag agctccgaag gtcatgcgaa agggttttgc gacgtttcct tttttttacg
      841 aaaattttta gcgtagtctt tttcaagttc agcccatgga atgagctctg ctttttgaat
      901 ccaacggtta gacggattca ttttgagacc tatcggttga ttgaaatctt caaaagccaa
      961 ttgaacggtt gtttgtaatt tatacatgaa aagcctccaa aattgagtgg tactccacac
     1021 cattctgatg gtgcataaga taaaaaatta aagaagtgcc tgtcatttgt ctgatttaag
     1081 ccaaggtttg acaaattttg gatattccaa ttaatactgt aagcggttat atttaactca
     1141 gggaggtaga tgaatgtgaa tacaaaagat ttaaaactgt tagaaaccat tgtacgatca
     1201 catgtgacaa actctcaaca attgaaatca ttagtacatt tttcgactag gcagataact
     1261 tactctataa aaaaaattaa tgatgaatta tcccttcatg gattgagtct gataaagcga
     1321 agtaactcag gggagtggtt atatgaagcc aatgatcttc aaaaactttt gatatacatg
     1381 aatcaagcaa tggtttttaa agaaggtgcg attcctgaat atataaacga tgatttggct
     1441 tatagtgaga taagaagata ttcggaactg ttatttattc tagccaaccc caaaaaagtg
     1501 acaacccaag aaataatggc gtttattggt atcagtaaaa atacggctag aaacgacttt
     1561 tctaaaataa aaaaagagtt gccaccagac tacactttga tatttgatcc ccaaaatggc
     1621 tatatgattg aatcaagcat ggcaaagctt catacattgc tcttagaagt tttcaattat
     1681 ttatttgaaa ggcatgcaac agttaaccgt tttgagtcac tcttttctga agaaagggaa
     1741 tttgttggtt tttttattca cacctacgaa caaactttta aattgaaatt ttctgataat
     1801 tcactaacca aactttatta tttgataggt ttatcacttt ctagttttaa aagaaggacg
     1861 aaaggacaga ttgattttag ttatgattta caggaattag atgagtttca aatgattcga
     1921 agctgcattg atcaacatta tcaacatgct gagatagcta tagattatga atggttgacg
     1981 cttctgtttt tatcattcaa tacaatttca aatgagtttg aaattttaga ttcaagaatc
     2041 tatcaagtaa ttatggagat gattacctta tttcaaagta aatcagctat tcggattagt
     2101 aataaagaag agttgaccaa tcgattgatg aatcatttac gacctgcaat ctatcgaatt
     2161 cgctacgaat taccatttga aatagatgat ttagctgaaa ttgttaagga aaaccatgaa
     2221 gttagtgcgg tacttaattt tgttaaagaa tccattgtac ctttggaagc atttttgcgt
     2281 aagacgattc ctgaaactga attatatttg atttctgttt atttttgttc ggagctatta
     2341 aattcttcta ttatcgaaac acctgttaga aaaaaggcaa tcgtcgtatg tagcaatgga
     2401 ttgattatgg ccaatgtaat ggcgaaaaca ttgaaagaaa tttttccaga tattcagttt
     2461 gttgctagtt cttcggtcag agagatggat aagtttgtcg aagatgttga tttgattttt
     2521 agcaatcaac cattgaaaag tgaaattcca ttatatatta tacaaccgat tatgaacgaa
     2581 gaagaaaaaa accgcttgcg gaaccgtgta ctcattgatt tttctacgaa taatatcagc
     2641 aaagagacta ggaaccttac tgaaattatt aagcgttact ctgctgttca aaatgaagaa
     2701 agtctacaaa ctgatattga gagattttta attaattatg caaattcact aatatatcta
     2761 gatcgtcaaa gtaaacaggc acaaaagata tcttttgtgg aattgcttgt ttctgaaggg
     2821 attattcttg tatctacgaa acaacttact tggcagaacg cgatggaatt gtcttgtcag
     2881 cctttattaa aggagcaggt aattaatcag aactatcaga ctattttaat gagtcaaatg
     2941 gcggatgaag aggcttatta ttttcttaat aatcgtattg ctattccaca ttcatcgcca
     3001 gaaaatgggg taattaaggg cggttgctcg ttacttatat cagttgatcc aattattttt
     3061 cctggaaata ggcctatcca ttttatttcc ccttttgcaa ttggaaagaa ggaaaattat
     3121 ttacttgcga ttaaccaatt actacaactt tctaaggacg aggaatatca gcaagaaata
     3181 cttctttgca cgacggaaga tgaagtgaga aaaaaaataa aaaatttttt aaggaggaag
     3241 caatatgtca ataacgaatt ttcctgaatt aaccatcatt ttaagaggat atagttttga
     3301 agaggcgatg tttgtcatgg aagaattggc tgcgatgaac acttcggtag gaattgaagt
     3361 aacaaccaat aatgatgact ttgcacgtat catcacagaa ggcaacaaaa aatttgggca
     3421 acaattattg attggtgcag ggacagtttt aacattttca caagcagatc aagcaattgc
     3481 tggtggtgcg aaatttttat taggaccaaa aaaattttca acaccaattt tttcgttggc
     3541 taaagaacgt ggggtggtaa ctgtgccatc agcttttacg ccatcggaaa ttcaagttat
     3601 gtttgatgaa ggtgccgata ttgtaaaggt ttttccagca gtcactgtag ggccaaccta
     3661 ctttaaacaa ctggccggtc cttttggtat acggccttta atggcagttg gaggagtgac
     3721 gattgataat attgcggctt ttaaaaaagc aggagttgtg tactttgggg taggatcaag
     3781 catgtttcaa aaagctgata tacaaaaccg caatgggcaa gctattcgaa aatcaattca
     3841 atcatattta gataaactag ctgaagcagg aagtgaagaa gatgagtctc attaaattag
     3901 aagaaaaaaa tattttgtta aatcaaagtt tttcaaacca agaagaatac tttcaaaaga
     3961 ttacaaagaa actgatgacg gaaaacatgg taaaggaggg atatctcgag tcaatcacta
     4021 agagagaaac aacttatcca acggggctat atattgggga acgctcaatc gcaattccac
     4081 atacagatta tcagttatca aatgttaccg ctattttagt atccgttctt aaagatagtt
     4141 tgcattttca gcgaatggat gatcccgaag aaatggtttc agtgaatgtt attattcacc
     4201 ttttattcga tagtcccgat aagcaaccgc aattattgaa ggagttgatg agtaaagtta
     4261 ccaatcagga tttcttacaa gcaattatgg aagcaaagag taaaacagaa gttttaactg
     4321 catttaatca ataaattgga gggaaataca atgagtaaaa aaacaattat cgtcgcatgt
     4381 gcatcatcaa tgattacatc tacaatggca gctaataaaa ttcggaaaat agcaaaaaca
     4441 aatggattac cagaacctcg aattttgcaa ctaaaatttt cagaagttgc aggtgcttta
     4501 gaatcaaatc aagttgactt tatcgttcct acagggaaat tacaagaaaa tgtaaccaaa
     4561 ggaattaaag ttatacctgg aacaccattt gttactggag tgaatgaaga aaaatctgaa
     4621 tctttggtgt tagaagcatt gaaagagctt gactaggagg gtgaaaaatg aatattgtca
     4681 ttgatgcgtt aaactggatc tcaagtttgg gtccgatgat tatgatgcct atcattatct
     4741 tgattttagg attatttttt agaattaagc taaatacatt actaaaatca gcgttgactg
     4801 tggggattgg ttttgcgggt gtaaatatta ttattaattg gtttatttca caggtaggtc
     4861 catctgttca gttaatggtc aaaaattggg gtattcaaac aagtattatg gatgtcggct
     4921 ggccagctcg tgcagcagcc acttggtctt ttcctctagc agcagttatg gtatttatta
     4981 ttttgggtat taatgtgttg atgctgattt ttaaaaagac caatacagta atggtcgatt
     5041 tctggagtta taatcactat atttttgtag ctgctttggt ttattatgca acggataaaa
     5101 atattatatt ggcggtaatc ttcggggcga ttgatgcaat tattagtttt aaactcgctg
     5161 attggacaac gccgttagta gaggattact ttgaactaga aggggttaat ttcccgactg
     5221 cgaactcagt tggttgggca cccttggctt ggctacttaa caaattgtgg gaccgcattc
     5281 ctggattaaa taaggtagat gctcgtcctg aaaaaattca agagcggttt ggttttgttg
     5341 gtgagcctat gtttatggga tttgtcatcg gcttggttat tgggttatta gcaaaagaac
     5401 ctgttgataa ggtattgata gtgggaatga gtacttctgc aacaatggtt ctaacaccta
     5461 aaatgatgca aattttaatg gaaggattat taccctttgc taattctgtt aagaaattct
     5521 tgaatgtaaa atttccagga aacaatttta caatgggtgt ggatgctgcg ttgacagttg
     5581 ctaattcaag tgccattgct acgggtatta tcatggttcc gattacctta gtgttggctg
     5641 ctattttacc aggtaacaaa ctcttaccta tttcagatat tgcctaccaa gcaatgtggc
     5701 tatctgcatg gccggtcgcc ttttcaaaag gaaatattat tcgcggaata ctttccacca
     5761 cgattataac agcttgtgtg cttttaattg ctactagttt agccggcata catacacaat
     5821 tagctttggc tgggggattc caattaccag caggaatgac acaaatatct accgaagatg
     5881 caggtacaca cattattggt ttcattttaa tgaagattgc tgagtttatt tcaaatattt
     5941 tctaatattc aggagggcat acagtgaaaa tttcaaaaat tacagtctat aaagtaaaac
     6001 caagatggat ttttgtcaaa atatctacag atgaaggtat tgaaggctgg ggtgaaatga
     6061 tttctggaac taagactgaa acagttgtgg caggagctta tgagatagga aatcgattct
     6121 tagttggtca aaacccattt gcaattgaac gtctgtttca ggaaatgcat cgttcatttt
     6181 tccgaggtgg tcctattaat ggaacgattg tctctggact tgaaatggct ttatgggata
     6241 ttaaagggaa agccttaaat gttccagttt atgaattact aggtggagca gcccgtgata
     6301 aaatcaaagt atattcttgg attggtggag atcgtccttc tgaagtagta gagcaggcac
     6361 aagatcgagt agatcgtggg tttaccgccg tgaaaatgaa cgcgacagaa gaattacact
     6421 acattgattc ttatgaaaaa gtagctgaag tagtggaacg tgttgcttca attagagaac
     6481 gttttggaga tacattgagc attggtgtcg attttcatgg tcgtgttcac aaaccaatgg
     6541 ctaaggtttt agcaaaagca ctggaaccct atcatccaat gttcttagaa gaagtggttt
     6601 tgccggagaa tgaagaacat ttcagtgaag tagccaatgc agttgctgta ccactagcaa
     6661 cgggtgaacg tttgtataca agatggcaat ttaaatcaat tcttcaacag ggagcgattg
     6721 atatcattca accagatgta gctttatgtg gagggatttt agaaacaaga aaaattgctg
     6781 caatggctga agcttatgat atagcagttg ccccccatgc accatatgga ccgattgcgt
     6841 tagcagctac attacaagtt gattcttgta cacctaatgt gtttattcaa gaacaaagct
     6901 taggtattca ttacaacaaa gggtttgatt tgttagattt tgtaaagaat aaagagatat
     6961 tccaatacaa agatggcttc gtagatcttc cgacaaaacc aggtttaggg ttagaaatgg
     7021 acgaagatcg agtgaaagaa attgcacaag aaggtttagt ttggaccaat cctaagtgga
     7081 aaaattatga tggtacaatc gctgaatggt aggagatgag tgcatgatca atacggttac
     7141 aggcccaatc gaagctgagt ctttgggtac aacatttatt catgaacatt tatatgtcat
     7201 tcctaatgag ttaagaaagt atgaagatta taccttggat gatatttatc atagtattgc
     7261 ggaagttatg gcttttaaac aagctggtgg aaaaactatc gttgatttga caccaattaa
     7321 ttatggacga aatccgttag ctttgaaaat gatatctgaa gctaccggag tgaatgtagc
     7381 atttgttacg ggattccata aggaagagtt tcagccaaaa tggttgacaa cgctcaatga
     7441 tgatgagatc tttgagttgc tttattccga aataactcac ggagtcactt cacaaaacct
     7501 tttgccagga gcaatgaaag taggtacgag taaaaatgag gttacattgg ctgaacaacg
     7561 aatactagcc atagccggtc aagtacaaaa aaaaacaggg attcccatta ttacgcattg
     7621 tgatgggggg acgatgggac tggagcaaat taattgttta acaaaaaacg gtgcgaaagc
     7681 aagtaatatt tgtttgagtc atgtagattt aactaaagat tttcactatt tagctcaaat
     7741 ctgtgaaacg ggagcaagta tttgtatgga tcatattggc cgagagttgg cgaatcatga
     7801 tgcagaccgc gtccaactaa ttaaacgact ggttgatgcg ggttatgaaa atcagatttg
     7861 tctttcaggt gatatgggac gaaaaaaata tttgccaact tatggaggaa cgcctgggct
     7921 tacctatatt ttaacaagtt tgaaagaaga actattaaaa gtaatgagtg aaccaacttt
     7981 taataaaatg attgagatga atccaattaa tatactgagt aagtgaagtc aattgaatca
     8041 ttcagaagaa ctaatgaaat tagttgcttt cgcagggtta attcttctct ctgggtttgg
     8101 aaaagagggc ttagcttcgg attccctagt taaactaggg gttttatcag gttattgtac
     8161 tgattataag tacctgaatt attcatgaaa tactttttcc gtcgttttat aggcagttgg
     8221 gtcaataaaa agatcagaaa aacgaacgag agactctcat aacgtgccac taaataaaaa
     8281 tcgtacgtta ttttaaaaaa tgggttgatt ctaggcagta ctttgtcggt ctatttttag
     8341 aaaaagaggt tctttgtcaa acatacatga attcgcaagc tcattcaccc tgaaggatga
     8401 aaggcagaaa ctgtagtata ctaatggtgg aaaggaaaaa aagccgccag caatttggaa
     8461 aagaacgtta tgtcgattgt caaactggct tgctttattt ctcgagaacc ctgaaatata
     8521 gccaatgagc atgtgtataa aaaattgatt ttatatgaaa taaagcactc ctttcctaaa
     8581 attattggaa aggagtgcgt tttttatgga aattatttag gttctttgtc aattgctatt
     8641 gttttgctca aatcgtatgt taggaaatgc ttcggcattt ccttttttgc gttattaaat
     8701 gatttcacag atgttctgtt gaatcaggac acgcatccgg agatgacgaa aagtccgaaa
     8761 tccaaaagca attcttttga ttactttgat gaggttattc tttccctcca attttccgtt
     8821 tgaataggga tattttaagg agtttgtgat tgcttctgtg tgctttttca gataacaaat
     8881 tgccttttta aacccatcat tcaaggaatg tggcaatgat tcaatgagat tgaaaaattc
     8941 agtaaaatct tttgcatcaa aagcagctaa taactcttga tagacttcat agctttgtcg
     9001 tagttggtta tctatagaga gtaggtaatc aagcacctcc gactccgtca tgtatttccc
     9061 ttggaagagg ggaaattgct taaaa
//
LOCUS       DACDRN010000089         9003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDRN010000089 DACDRN010000000
VERSION     DACDRN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.089"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..105
                     /locus_tag="IWJ80_002469"
     CDS             <1..105
                     /locus_tag="IWJ80_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9462154.1"
                     /translation="DFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(173..466)
                     /locus_tag="IWJ80_002470"
     CDS             complement(173..466)
                     /locus_tag="IWJ80_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP9462155.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(494..1159)
                     /locus_tag="IWJ80_002471"
     CDS             complement(494..1159)
                     /locus_tag="IWJ80_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP9462156.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1299..1931)
                     /locus_tag="IWJ80_002472"
     CDS             complement(1299..1931)
                     /locus_tag="IWJ80_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9462157.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1938..3005)
                     /locus_tag="IWJ80_002473"
     CDS             complement(1938..3005)
                     /locus_tag="IWJ80_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9462158.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(3002..3733)
                     /locus_tag="IWJ80_002474"
     CDS             complement(3002..3733)
                     /locus_tag="IWJ80_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP9462159.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDIEVIRI"
     gene            complement(3901..4380)
                     /gene="greA"
                     /locus_tag="IWJ80_002475"
     CDS             complement(3901..4380)
                     /gene="greA"
                     /locus_tag="IWJ80_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP9462160.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4516..5691)
                     /gene="mltG"
                     /locus_tag="IWJ80_002476"
     CDS             complement(4516..5691)
                     /gene="mltG"
                     /locus_tag="IWJ80_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP9462161.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5856..7016
                     /locus_tag="IWJ80_002477"
     CDS             5856..7016
                     /locus_tag="IWJ80_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9462162.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(7056..8003)
                     /locus_tag="IWJ80_002478"
     CDS             complement(7056..8003)
                     /locus_tag="IWJ80_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9462163.1"
                     /translation="MLNYLITGGAGFIGSTLANYLGKENNVVVIDDLSMGKKENLDES
                     KHITFIEGDVADKNLMEKVMKEYHFDYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(8036..>9003)
                     /locus_tag="IWJ80_002479"
     CDS             complement(8036..>9003)
                     /locus_tag="IWJ80_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP9462164.1"
                     /translation="YEKYYNARHRIRIGFLESRLFGTFSDINNAAIASLVVIVLILFY
                     LYNKKVTGLNKIIFLINLPLQFIYIVLSGSRSTYVVSIIIVAISSFIVVLKRNGTKKD
                     LKNYILAIVSSIGSVLILFALFNATKILFQELLPHLKEINFFNFTERHKELMNNSQHI
                     NNTQTPDNTLVRKDVAENTDISNARITIWKSAWEIFKTSWLIGVSPKNVVSYAQSIIP
                     DGYIAKVGIAIHNAYLNVLTSTGILGIIPFMAFLIKSFVLNVYRIIYNKCIGKDYHFV
                     YCLVILSYALYAALNNELVYENTVGTFVFWLFLGRVNSKNNSSYF"
BASE COUNT         2753 a         1838 c         1382 g         3030 t
ORIGIN      
        1 gattttcaaa agaaattaat ggctattttt ttaaccaatc tgaaaagcta ctttgccacc
       61 catgagcttc aaaccaatca agtaaaattg tctgcaggat attaatagct gaaaacaata
      121 ttttcaaaag aaaaaagaga aggtgacaaa aattgtgtca ccctctcttt ttttattgtt
      181 ttttaaagtg ataggttggc tcctccaggt tgggatcata atcaacaagt aaatcatatc
      241 ctttaaagaa ccattcatct gcttcctcga taaaaaaaag tgtcccatcc atttccactt
      301 tctttacagg atgttctggc tgttcaactg acattccaat cgaaaaacca tcatgaacct
      361 ctgttttgcc gtagactttt ccaaaaaaac gtattccgta agtttctggc agatcaattt
      421 ctgagtcaaa ccacttttgt gcttttggcg ttaccgttaa tttcatccca acaacctact
      481 ttctcttttc tttctattga ttcatcttat cgtctagaat atccacatcg cctgtctctc
      541 caataaccag tagattatca ttttcacgaa catattcatc tgctgcagga gaagcgataa
      601 ccgtttgatc tactcttcgt atggcgacta ctgttaagtc aaaacgttgc ctgaaattta
      661 attcagctaa tgttttgttg aaaaatttag gattggagac tcggatttct gctaaagaat
      721 atttatctga cagctctaag tagtctagaa tattctttga tactaagtta tgtgcaatcc
      781 gtatccccat gtctcgctca gggtgtacga cacgatctgc tccgatcttc tctagtacac
      841 gggcatggta ctcattttgg gcttttgcta aaatattcgg tacgcccatt tcctttacca
      901 tcagagtaac taagatgctg gcttggatat cttctccaat agcgacaatt acatgatcga
      961 aatttcttat acctaatgaa cgaagtgtca tctcatcctg tgcatttgcc acaacagcat
     1021 gggtggctat attcatatat tcattgactc gatcttcatt actgtcaata gccagtactt
     1081 cttgtccagc ttcgatcagt gtacggcaga tgcttccgcc aaatcgtcca aggcctacaa
     1141 ttgcaaaatt ttgtttcacc attttcctcc tttagtatct tcttttctca gtataacaaa
     1201 gcctttccaa aaacaaaagc aagcatcatt attgtaaaaa agccaagaaa aataatagtt
     1261 attttctcag cttttgatta tcctctttat catcgttttt atttcaccaa accatgttta
     1321 aacgcataga tagctgcttg agttcggtct tctacctcta acttagctaa tatattcgac
     1381 acatgcgttt tgactgtttt cagtgtgata aacaattcat ccgcgatttc ctgattactt
     1441 ttcccttcag aaatcagcat caaaatttca ttttctcgat tcgttaattc ttcatgcaat
     1501 actggatcat ttcgccgact cattttttcc atcatttttg ttgtcacttc aggttctagt
     1561 actcgctcgc cgcgttgcgt agcccttata gcatcagcta tttcatgtgc tgtagacgtc
     1621 ttcaacaaat atcctgctgc tcctgcttca attgcaggat aaactttttc atcatcaata
     1681 aaacttgtga cgataatgat tttggctttt ggccaatctt ttaagattgc ttttgttgat
     1741 tcgatgccat ccatttcttc catgactaga tccattaaaa tgacatccgg tcgaagtgcc
     1801 atcgcctttt catagccttg acgtccattt tcagcttctc caatcacttc aatgtcttct
     1861 tggatagata aataagaaga gacgcctaag cgtaccattt catggtcatc tactaataaa
     1921 acttttatca tctttatcta tgcctccttc atcaaaggga ccttggtctc cacgctcgta
     1981 ccttgccctt taaaactaat gatttttact gttccaccaa taccagctac tcgttctttg
     2041 atattgttca atccataact tcccgttttg gtttcattca tattaaatcc tgtcccgtcg
     2101 tcaatgatac gtaataaaag attgttatct attttatgca aatagacttc taattcatta
     2161 gccttagcat gtcttaatgt gtttgataac aattcttgaa cgattcggaa caagtgatcc
     2221 tcaatggaac tagttaattt tacatcttct acctcccatt ttagcgaaat ctggatttta
     2281 ttctgcaatt ccttcaataa ctgctcaatg ccttgtttta aacttttttc ttctaaattg
     2341 actggacgca gatgaagaag cagcgcacgc atctcagact gggaagcgtt gatgatctct
     2401 gccaccattt tcaactgctt acggaacatt tctggtgtct cacttttttc tgcttgctcc
     2461 gtcaatgccg acatcatcat catcgctgca aataattgct gtgaaactga atcatgaagc
     2521 tcacgagcta atcgatgcct ttctaatttt aaaatctctt ctttggtttg tccatctaca
     2581 tattgaggac ggctagtcat aatctgtaat tcactggata cttcgatcat tttttctttt
     2641 attttagtaa tttccatatc aatatctttg atgtacagat catccgacgc gcttgggatc
     2701 ggctgatgca aaagtttgga ttcataattg ccagaagaaa gtgcacgaag cttttcttcg
     2761 attttcccgt attttgcctt ttgaataagt gataacaata gaaatacgat caatcctaca
     2821 cctaatgaaa ttgccatcaa atggaagata agcggaacgt ataataaacg cgcacgcagc
     2881 aattctcccc accaatgact ttggttactg gcataaaaat aagtgaaaag tgaaaataga
     2941 ataataagga aagccgcaat ccccgaataa acagctagca ttgctttgga tattcttccc
     3001 atcatattcg tatcacctcg atatctccaa ccaaagtatt ggtaatgatc ttcaatctgc
     3061 gaggattttc atcataatca ctgctgcaga ttttgatctg ttcgtttttt aaggaaaact
     3121 gctcttcttc aaacaagaca tttcctacta atgtcgcatg ctctaaacga atagccactc
     3181 ccaggggtac taagatccgt gttctgccta tcccttttct tacaatcaca atattatcat
     3241 cttttggcaa aagtgtattg cccaaatcga tgatagtatc accagaaatg atggcaatgt
     3301 tgatatcatc ccattcatag acgtcgtttc caatacgttg gttaccaaat aattgctgtc
     3361 ttttacgctc attattatgt gtttttaatt gctctgtttg gaccatcatg atctgttttt
     3421 ttcgccaaaa agcatttttt gtcaaatcta ttcctgaaat ctctacacct ttcagcccga
     3481 taaataaaac ggccaataca accatcatcc ataaagcagg actgttgact aagctaaaaa
     3541 aaatgaccaa gctccctaag atcaattgaa aattttgaaa tttagtacga ggatatttgc
     3601 gcatagctag atatatattg aaaataccga atattactaa aagcaaaagt ccagtattgt
     3661 ttacgatttg ccaaacagca aataacagca gcagtgcttc gactaccaca aaaaaacgcc
     3721 aagaactatt catcgctttt tctccctcct gacacattct catttcatca tacctatttt
     3781 tttgactgtt ggctatagga ctttagatgt aagatcgttc agtgaaacct gcaatactca
     3841 aagagaaaag atctagagtg cgtcatcagt aagatgaact ctctagatct ttcagtcaga
     3901 ctacgcttgt tctactttga cgattttaac taacatatcg ccacctggtg tagcaatggt
     3961 tacttcatca tttttacgtt tgccaatcaa tgcttgcgcg ataggagaat cattggaaat
     4021 ctttccagaa aacgggtctg cttcagcact tccgacaatc gtgtactctt cttcatctcc
     4081 atcaggcaac tcgatgaacg taactgtttt accgatcgat acctcatctg catctacgcc
     4141 gtcattatcg atgatttgtg caaaacggat catattttca attgttgtga ttcttccttc
     4201 aacaaatgct tgttcatctt tagctgattc gtattcggag ttttcggaaa gatctccgaa
     4261 accgcgagcg attttgattc gttcaatgat ttctccgcgt ttaactgttt ttagttcttc
     4321 taattctttt tctaattttt ctttaccttc tagagtcatt ggataaactt tctctaccat
     4381 gtgtaacgtc tccttttttc gataaaatac cgcaaaaggt aatcttttga ggaatatagt
     4441 ccaaaaaaga ttaccacgct tttcttaaaa tgtaaatgtt tttctaaaaa tgatcaatga
     4501 ctagcccctg tttttctaag aattatttac atatttttgc actagttcca tatgttcttc
     4561 atacgtttta gcgaaataaa catttccagt gctcgtatct gctacgaaat aataatagtc
     4621 attttctgct ggttctaata ctgccttgat tgcctcttcg cttggattgt caaaaggacc
     4681 aggcccatag ccggtattgg tatacaaatt gtatggtgaa tctacagctg tatcttcata
     4741 cgtcaccaat tctttatgtt ctcctaatgc atataaaaca gagatatctg actgaagtgg
     4801 catatttgcc ttgattcggt taaagaagac ttgtgcaata tttttacgat cattttcttt
     4861 tacgccttct ttttcaacta gagaagccaa agttaaaact tcttgtacag tcaaattttt
     4921 ctgtttgata gctgaataat agttttgcat gaccgtattt gttttgttca ccatctcaat
     4981 cacaagatcc ttaagcgaag tactcgtgta gtaatcataa gtagctggga ataagtagcc
     5041 ttccaaacgg tattttgtgt tctcagcttt agaggcactt tccaacaatt ctgggaattt
     5101 ttgatacaac tcgttaaaga aagtctcatc atttactaaa tctaaaaatt ctttcttgtc
     5161 tttaccagtt accttcgcta cccgctcagc aatctgatcg atgtcgtagc cttctggaat
     5221 ggcaattttc ccatctgcaa cttttgttgg ctcagatgtg ccgccttctt gcaactgctt
     5281 gccaatctca tcaagagtca tattaggcgc taactgataa taaccagctt gaaagccagt
     5341 taagttttta aattttgtat aataattgaa aacaatccca cttttaataa tattgtcttt
     5401 ctccaaaatt tcgccaatct gcttattaga agagccgctt gggatttcca cttggactaa
     5461 ttggtcgtcc gatttatcta aaggcttcaa cccgctatct acataacgat atacggtaaa
     5521 cccgagaaat ccgccaataa tcaataatgt aagagcgatc actaaaatga tcttcctcac
     5581 gatcttatct tcttttttac gcagatttct ccgcttgtcg attatatctg ctgtagctgc
     5641 cttactggct tcttttttgt ctttgcttgg gtgttgtttg tttcctgaca aaacaactcc
     5701 tccttactat catcttcgta actctgttta ttatacatga aatttctact gccgtgaatt
     5761 attttaccaa atattatgtc aaaattgtaa gtggcaaatg ctctttttga ggattacaat
     5821 gaaaaatagt taaaaatgga taggaaggat ttaatatggc aaaaggaaaa catcaaaaaa
     5881 aacagcagat ctctctttct tttctcgcag ttttgttgct ggctgtcagc ttcttttcaa
     5941 caggcatcat tacaggatgg caattaacaa aacaacctca accacaaatt tataaaacgg
     6001 aacccgcaca acttgcttcc aatttaacat ctaatgagca gatgcttgcc agcaaaattg
     6061 atcaattact gcaaaaatca gattatatcg gttcgattta tgtacgcaaa aataatcgag
     6121 tgatattaga aaaaggatat gggtacgcaa atctacgcat aaaagaatcg aattctcctt
     6181 ctctttacta tcaaatcggt tcgatccaaa aagcgatgac tgctttactt atcctaaaac
     6241 aaattcaatc tggcaagata acttttgaca caaagctatc tgaattttat cctcagattc
     6301 ctggaagtca gcaaatctca atcaaaaata tgctttacat gcgctctggt cttcatcgaa
     6361 ccgcttctcc aaaaaaacct atgtcagata gtgagatcat tcagtttgcc cttcatcatt
     6421 tgaaatttac tggttatgat acgtatcatt atgaaccatt aaattataca ttgcttacag
     6481 gggtattgat caaactaatg aatcagcctt atgaaactct tttaaaaaaa gaaatcattc
     6541 ggccactcca tctagaacat acagattttt atgaaaatgt aaaaaattcc cctcagcatg
     6601 ctgtatctta ccaaatgtct gcaactgacg attattcaaa agcattggtc gagcctgaaa
     6661 cagacatccg aaatgaatta ggcacaggaa atataagtat gtctgtttat gacttgaaca
     6721 aattctttac cagtgttctg tctggcgata ttataccaaa agaactactt ttctcattgt
     6781 ggcaaaataa tggtgataaa catccttaca gcggcggtgt ttacagtgga aatgattata
     6841 tccttgctca gggaaatatc aatcgattcc atgcagtagc ggcgatgaaa aaagacacaa
     6901 aagatgcaat cgtcatggaa agtaatatcc aatctgataa aaacatcaaa ttgccggcca
     6961 ctgatttaag aaatcagatt tacgaactga tggaaggtgt taatttaaaa agctaagtcg
     7021 agacaaaaaa accagcagga tgctggtttt ttgtttcaat cgatttctgt ctttaaataa
     7081 cgttccattc cgttcaatat atcaaatttg ggttgataac ctaaagaacg gatctttgaa
     7141 atatccgcta aagaatcacg aatatcccct gaacgctccg cttcatattt aagcgctagc
     7201 tctgttccta agatttgatc aattgaatga atcaattcaa gtagcgtagt gctttttcct
     7261 gtaccgacat tgaattgctg tcctaaagct ttttcttcct tagcaacaag taaaagtgct
     7321 tgtataacat cttctatata aacgaagtct ctagattggc taccatcacc aaataaagta
     7381 aattctgttt tttctcctgc caattgtttt ttgtatcggt ccactagaat cgaaataact
     7441 ccgctatatg gagagttagg attttgattt ggaccataaa cgttaaaaaa tcggacagca
     7501 ctagtcggaa catcatataa atgacagtaa tccaaaacat attgttctgc tgcaaactta
     7561 tcgatggcat atggtgttaa aggacggatg accgattctt ctttttttgg caacgttggt
     7621 tcatcgccat aaactgcagc tgatgaacta aataccaatc gttttaaacc agattgatat
     7681 tttcgtacca attcaagcaa taaaagcgca ctgtcaaaat ttacacgatg agtttctaga
     7741 ggacgttcta cagaatctgc cacactagct acagcagcta gatggaaaat ataatcgaaa
     7801 tggtattctt tcatgacctt ttccatcaaa tttttgtcag cgacatcccc ttcaataaac
     7861 gtgatgtgct tcgattcatc cagattctct tttttcccca tcgataaatc atcgatcaca
     7921 actacattgt tttcttttcc tagataattc gcgagcgtcg agccaatgaa gccggcgcca
     7981 ccggtaatca agtaattcaa cattttttct cctcttttca agataaaatt tattactaaa
     8041 aatagctaga attatttttg ctattaactc ttcctaaaaa tagccaaaaa acaaacgttc
     8101 ctacagtgtt ttcatagact aattcattgt taagagctgc gtatagtgca taagataaaa
     8161 taactaaaca gtaaacaaaa tgataatctt taccaatgca tttattatag atgattcgat
     8221 aaacattcag cacaaaactc tttattaaaa aagccatgaa aggaattatt cctagaatac
     8281 ctgtcgatgt taatacattc aagtatgcat tatgaatggc aattcctact ttcgcaatat
     8341 atccatctgg aataattgac tgtgcataag atactacatt tttcggagag actccgataa
     8401 gccaacttgt tttaaatatt tcccatgcac ttttccatat agtaattcta gcattagaaa
     8461 tatctgtatt ttcagctacg tctttcctaa caagagtatt atcaggtgtt tgagtattat
     8521 taatatgttg actattattc attaattctt tatgacgctc tgtaaaatta aaaaaattta
     8581 tttcttttaa atgaggtaat aattcttgaa aaagtatctt agttgcatta aacaaagcga
     8641 ataaaatcag aacagaacca atagaagata ctatagcaag gatatagttt tttaaatctt
     8701 tttttgttcc atttcgtttt aacacaacaa taaaagatga tatggcaact attattatac
     8761 ttaccacata ggtagatcgt gacccactta atactatata aataaattgt aatggaagat
     8821 taattaaaaa aatgatttta tttaatcctg tcactttttt attataaaga taaaataaga
     8881 ttaggacaat aacaactaaa gatgctatag cagcattgtt aatatcagaa aacgtcccaa
     8941 acagtcttga ttctaaaaaa ccaattctga tcctatgtct agcattataa tatttctcat
     9001 agc
//
LOCUS       DACDRN010000090         8365 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDRN010000090 DACDRN010000000
VERSION     DACDRN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.090"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(233..910)
                     /gene="nth"
                     /locus_tag="IWJ80_002480"
     CDS             complement(233..910)
                     /gene="nth"
                     /locus_tag="IWJ80_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP9462165.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(917..1615)
                     /locus_tag="IWJ80_002481"
     CDS             complement(917..1615)
                     /locus_tag="IWJ80_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP9462166.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1883..3067
                     /locus_tag="IWJ80_002482"
     CDS             1883..3067
                     /locus_tag="IWJ80_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP9462167.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3073..3516)
                     /locus_tag="IWJ80_002483"
     CDS             complement(3073..3516)
                     /locus_tag="IWJ80_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9462168.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3742..4029)
                     /locus_tag="IWJ80_002484"
     CDS             complement(3742..4029)
                     /locus_tag="IWJ80_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462169.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4200..5552)
                     /locus_tag="IWJ80_002485"
     CDS             complement(4200..5552)
                     /locus_tag="IWJ80_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9462170.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSDPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQEALGEIETAQ
                     "
     gene            complement(5824..6270)
                     /locus_tag="IWJ80_002486"
     CDS             complement(5824..6270)
                     /locus_tag="IWJ80_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9462171.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6506..7432)
                     /gene="cysK"
                     /locus_tag="IWJ80_002487"
     CDS             complement(6506..7432)
                     /gene="cysK"
                     /locus_tag="IWJ80_002487"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP9462172.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7572..8365)
                     /locus_tag="IWJ80_002488"
                     /pseudo
     CDS             complement(7572..8365)
                     /locus_tag="IWJ80_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
BASE COUNT         2269 a         1720 c         1400 g         2976 t
ORIGIN      
        1 tggaatactg gacttatttt ataggtatat tggaaggcat tattttctac atcgggtatt
       61 ttcccaataa aaataacaac ttttattttt atcgcaataa attgtaataa gttaccatcc
      121 tagctactga gtatgtacat ttactttttc atgcaaaaaa gataaacgaa acagcttttc
      181 tccataataa caagataatt catacattta tttgcaatta aagaaaccac gtctacaggc
      241 gtggtttctt ttttagcgcc ttttctttta gacgcatccg atttttccca tcttgacata
      301 ttgatagcaa aggacatacc tcgcacttgg gatttcttgc cgtgcaatga tatctaccaa
      361 aaaaaatcag tgtatgatgt gtctttaccc ataattcttg tggtactttc cgcatcagtg
      421 tttcttctac ttccatgacc gtagcatcta gtttgcagat cctcaggcgt tttgagacac
      481 gttccacgtg ggtatctact gcgattgctg gtatcccgaa tgcatccccc aatacgacat
      541 ttgctgtctt tctgcctaca ccaggaagag acatcaattc ttctcttgac gttggcactt
      601 gcccatcgaa acgttcaatc agctgctgtg cacacgcttt gatgtttttt gctttatttc
      661 gatacaaacc aatcgttttt attttaagaa tgatttcatc gattgaagct tctgctaacg
      721 catcgggtgt cggaaaagag gcaaacaaat ctggcgttgc tttattcact gatacatctg
      781 tcgcctgggc acttaagatc actgcaatca ataactcaaa tggattattg tgcttgagtt
      841 ctccatgtgc ttcgggaaac attccataca tcgtttctag agcttccatt gttttttgtt
      901 tgcttaacat attccgctac tcctccaacc aatttttcag tgagactttc ggcaattctt
      961 cttgttgagc tgactgttct acttctcttt gaagaagctg ctgttttctt cgtttttggt
     1021 cttcttccac ttgttgtttt gtacgtagat tttttctttc ccacgatagc aaaatgcggt
     1081 caacgtaatt aaaactataa gcctgattta atacggcttc ccgcagtgct agcttcaaaa
     1141 tttccggctg ataatggtct tcttctagcc attgtccgat ccgctggaac tcgatcgaag
     1201 aaagcgggcg tccaaattct tgttcaaata attgatacac tgactggatt tctttttctt
     1261 gtgttttttc ttcgtatttt ttttcttgtg tttttaaata ctcacctaat agatcataga
     1321 tggggtaaag attatatcga tctgcttttt taccgttgac aatcgtcgtt tctatcttga
     1381 taaatccatt tgttacgaga cgatccagta tttgaaaaat ctgatcttgt ttgattccca
     1441 tatccatagc aatcatctgc aaatcaggga aagtatctcc ttcaagctgt gccatatgca
     1501 actgtagaac aaataaaaat tcttcagatt gtagaccgat cctatgataa tttttaagta
     1561 aaagattaga tatgactgtt tgtccagcgc ttaagtaatt ttctaaattc aacatgattt
     1621 ctcctccgct caatcacagt atacgtaaaa aaaacatggg aagcaagaac aggtttcttt
     1681 tctatttact tcactgatcg gtcagattta caatgtcgtc tacttgattt ttgtttgttc
     1741 atgtttttga tgcgtatctt cacttttcgg aataaataaa ttaagtagat ttcgctgttc
     1801 cttagaatgt tgacaaggaa acaaaattac tatacactat ttttgtttcc aagttaacat
     1861 tattgcataa aggagttttt acatgtacag tttcacaaat aaacattcta aagtttttgg
     1921 atacgtctct gtcttattca ctggattagg atttactatc gtcagtcctg tacttccgtt
     1981 tttaacgctg ccttattctc actccataca tcagcaagct ttctatatca ctttgctcat
     2041 gtctgtttat gctctagctg ccttcttatc tgcccccatt ctcggtagct taagtgatca
     2101 ttttgggcgt catccgatcc ttattatcag tttattagga tcaagtatcg gatatttgct
     2161 ttttggttta ggaaattcga tatggatgtt gttccttggt cgtatcattg aaggattgac
     2221 ggcaggagaa atctccacct tatatgctta ctttgccgac atcacggaac ctaatgaaag
     2281 aaccaaagtt tttggatgga tgggtgcttt agtcggtata ggaacaacgc tgggtcctat
     2341 tatagggggt ctattagctg agttggggaa ttctgttcct atatttattg gagcactctt
     2401 tactttctta aatgccgttt atggctacac atttatgcta gaaagccttc caataaaaaa
     2461 acgttcagta gatttaagtt tctcccatgt aagacctttt caccaattac aacaattatt
     2521 caagatttca tccgttattc ctttattgac agcaggtttt gctgtttggt tggcagctgg
     2581 ctctcttcaa tctatctttt ctcaattttc gattgacgcc tttcagtgga aggctgggtt
     2641 ggttggttta agcttctctt tgattggaat cttggatact gtctcgcaat tgttcattat
     2701 gcctcgattg ctcaaaaaat tttcagaaca gcagatcaca agaataggga tgttcagtga
     2761 aatcctcgcc tatctattca tcacattatc tggaattctt ctactgccga ttttgttttt
     2821 atttgggatt atctgttatg gtttcggtga ttctattttt actcctgtat tcaatggcca
     2881 actatccaat tctgtctcag ataaccagca aggtcttgta atgggcggta cccaaagtat
     2941 acaatctcta tcgcgcgtca tcggcccgct aatcgcagga cagctttatg ctgcagcacc
     3001 atttcttccg acggtattcg gatttttgct tatgattttt gcctattatc actacaataa
     3061 aaaatagtag cttcatttgc tacttcgtat aaattctctg tatcgaccga taaaacgaaa
     3121 tactgtgtca aattcttctt ttgacatatt ttcaaataca gtagcatctc gctgtaccca
     3181 tttttgatgg agttgctcgt gtctattcct tattttttgt ccgattggcg tcaattgaaa
     3241 atagatttct tttttattct cttcgctttg ataagtgttt atgatccctc tagctatcag
     3301 tttttttgtt agcttactga tggctcctct agtcatgtaa ctagcattcg ctaattttgt
     3361 cacgttagga tcagggattt tcccgatatg ttcaatcact tctagttcat ttagagtaaa
     3421 tcctgcaaat tctttttcca tttctggttt attacgatga ataatctcat tagctaaatc
     3481 cagatattct tctattagca gttgtctttt atccatttct acaccttctt tttctttcat
     3541 tatattgttt ttttgtttcc tattcaacaa agataaaaat attttgtagg aaaacccaat
     3601 ctgaaaccaa gaaaaaaagg ggttgacaaa gcttgtcata accccttttt ctgattaaaa
     3661 gatagtaaag agacagtcat cggtaattgt gatgattttt tgtcatcact taaggatgag
     3721 cggccttttc aaaaactgtc tttaaagatc atcatcagtt tctagcacat atggctgacc
     3781 ttcatatgtg tactctgtat tcaatgtgcc gtcattttca tctgagtaga tgcgtctact
     3841 gaaaggaaaa cgatgagaaa cattatcttt ggcgtcattt gctggcatcg tgtaatattc
     3901 ctcttgcgtt tcattcaact gagagagtac ttgatctgcc aactcatcaa tattgatcgt
     3961 taatttcttc gaagcacttt tataatcttc taaaaatgca tcttgttgga caaaatattc
     4021 tttattcatc ttctttctca cctttctttt caaaatcaaa taaagctgct tgttctttta
     4081 tgtaacagct ttatctttgc tgcttattgt gacaaaaaat tcgaagaaag acaactaatt
     4141 gtacttttca caaaaaaaga cagagggtcg tgacaaaatt gtgtcacaac cctttttctt
     4201 tattgtgcag tttcgatttc gcctagcgct tcttgtgcaa gtaggttcaa gtaattccat
     4261 gggcggtcaa aatgtggttg gaagaagaaa tctgaaagtg cgagatcttc tacagtcatt
     4321 ttattttgaa cagctaaaga catggtattt gctgattgtg taatgtcgta tttggacatg
     4381 aactgagcac ctacgatacg attcgttcct ttttcataga ctagctccat cattactttt
     4441 tcagtagttg gcatgaactc tggacgataa ttatcttcaa atacagtcgc ttggacttct
     4501 agatcgttca aagttgcgct ttctttggtc acacctgtcg aaccgatcgt ccagccaaat
     4561 aggtaaagtc ctgaagttcc ttgagtacca cgatacgcca ttttatgatc tgtcaagtta
     4621 tttcccacaa gtaaaccttg acgaacggca tttgttgcca aaggaatata gttttgcgtg
     4681 ccacttggat tgtagttgac tactgcagag tctccagctg caaaaatgtc tggatcactt
     4741 gtctgcatgt attcattcac tttgattgcc ccgtttggca gtgtatctac ttttccttcg
     4801 atcagttttg tatttggacg gaagcccaca cataaaatga ccatatctgt atcaaatgta
     4861 tctgttggtg tagctacttg tttcactttt ccttcttcat ctgtgataaa ttcagtaaca
     4921 ttttcgccta gcgcaagagt aacaccgcgg tctgtcaatt ctttttctaa gatatctgtg
     4981 aatggtttat ctagatattt attcaagatg cgatctaatc catcaatcaa tgtcacttgt
     5041 tttccagatt cgacaaaagc ttctactaat tcaataccga tatatccacc accaacaatt
     5101 actacacgtt ttgcttcttt cgcttgtgca ataatttcat ttgcttgatt gtagtttttg
     5161 cataggagta catttttcgc atcgattcca ctaatcggtg ggataatagg ccatgaacca
     5221 gttgtcatca ctaatttatc ataagaaacc gtttctgttt ctcctgtttt cagatctttt
     5281 gccgttactg ttttattttc tgtatcgacg tctgttacat cgtgttccat tttaactgtt
     5341 gctcctaatg aagataattc ttcaggattt gagtagaaca agcctgctgg gtcctttact
     5401 actccgccga cataaagtgc gataccgcaa gataagaaag aaatattgtc atttctttca
     5461 tatactgtca cttctgcttc tggatgattt tttaaaatac tttttacagc tgcagtacct
     5521 gcatgagtac agccaattac tactactttc atttatggtc cctccgatta acattttgtc
     5581 cttcgacaaa actactataa cagaaataaa acggatacaa cagtttaatg cttgtgaatt
     5641 cacaaaggtt tgtttgtagt ttcattcctt ttcaagcata agaacccaat atctacaaga
     5701 ttcttattgt gataatattc actttgatat ctaagattgt gaataaaaga caaaaaaata
     5761 aaaaagctag cgttttttta cacagctagc tttttttagg tataacactt atagaaactg
     5821 tttttacatt tgcgcctttt ttgctgactc tttacaatcc ggacagagtc catacaattc
     5881 caaccgatgt tcattaatct caaaatcagt cagtctgctc gcagccattt ctatatcttc
     5941 aagccctggg taatgaaaat caacaatctt accgcagttt tcacaaatta catgataatg
     6001 acgctttgaa ctgaaatcaa atcggctaga agcatcgccg tacttcattt cttgaacaaa
     6061 tccaatcgaa gtaaataagc gaaggttatt gtaaacagtc gcaacactca tatttggaaa
     6121 ccgatgttca agtgctttat aaatctcatc agccgttgga tgggaatgat gttcaatcaa
     6181 atattctaaa accgcgtacc gttgaggtgt gattcgaatg ttctcttttt ttaacttttc
     6241 gataacctct tcgactaact tattttccat gtaaacaccc cacgttgtta ttctcgtctt
     6301 atcatactat ttttacattt taaaatccac ttaacatgaa aagaaaattc aattttattg
     6361 tattttaatg aaaaaagacg taaaacagtc gcgttcacta ttttacgtct ctattcaaat
     6421 aatttttctc atcataaatt tttgtgtgtt cttttcatct ttaaaagttt tttacataaa
     6481 cctatctgga gaaaaaccaa gtgccttata tctcttggta aagggctgta gataaataac
     6541 gttcaccatt gtcgggaacc acagctaata ccttcttgcc ttttcctaat tcttttgcga
     6601 cttttagcgc ggcagcaatt gcagcgccag atgagatgcc tactaaaatc ccttcttttc
     6661 gcccaacttc tctagctgtt tccatcgctt cgtctccact aatagaaagg actttatcat
     6721 acacagaagt atccagtgtt ttaggaacaa aacctgtacc gattccttgg attttgtgag
     6781 gtcccgcttc tttcccttct aaaatagcag attctgctgg ttctaccccg attaattcga
     6841 ttttcggata cacacgtttt aattctcggc cagcgccagt gatcgttcct cctgtaccga
     6901 tacctgcaac aaacgcgtcc aagccattta ctccaaatgc ttgatgtatc tcaggaccag
     6961 tcgtcttctc atgcacaaga ggatttgctt cattttcaaa ttgtaatggt aaaaagtagc
     7021 cgttttcttt tgccaaacgt tctgcttctc tgattgatcc agaaatacca tccgcaccag
     7081 gagtcaaaat caactctgcg ccgtaccctt tcatcaataa acgtctttcg atactcatcg
     7141 tctcaggcat cacgatgatt actttatatc cttttgcaac tcctaccatc gacaatccga
     7201 ttcctgtatt tccagaagta ggttcaatga tagtatcacc tggtttcagt agaccatcgt
     7261 gttctgcttt ttctatcata cttaaagcaa tacgatcttt tacacttcct cctggattaa
     7321 aaaactcaag ctttacaaaa acatctgctg aatcttctgg tacgatttta tttaatttga
     7381 cgattggcgt tttgccaatc aattcggtga ctgagttata gatagctgtc atcttcaatt
     7441 cctccttgtc tttgctcttc ttacttatca taatacaatc tgttcattta tggcgagtga
     7501 ttcgtctttg actagcaaaa aaaagacaaa gatataccat atatggtagt ttctttgcct
     7561 ttttatctaa atcaaacgtt tagttcttga ccaactaata aagaataact ggacatagac
     7621 atgccatttt tcgccattag atcatcaaca gacatgccaa atttttgtgc aatatcccat
     7681 agcgtatcac ctgacacgac tgtatatgtc tgaccattat ttgtatttac atctgttgtt
     7741 gaagatgatg acgttgaagt cgtcgtagta gtcgttgttg ttgtacttgt ggtagccgaa
     7801 cctgttgatg gcgtatcaaa acgggttaaa ttataagtat taatcaatga gttcaatttg
     7861 gccccataat ttggatcggt agcataacga cctgtcaagt acgctgttgc atcataaaaa
     7921 cttgttgtat tgcttttcca cacgccagca taatgataat cgcccgtgga aaaactggtt
     7981 gaacgaagaa cattcgcatg gtcttggaag gattcccaat aagaagaata ttttcggaat
     8041 ggttcattca tcgttaccca ttgtccgtta catattcttt ggttgccata taaacgacct
     8101 gtccgttata gcttcctttc acaccgaata gattataatt tggcgcacta gctaatccgg
     8161 aagtgccata tgcactttcc aacaacgctt gagcgatcat gactgaagca tacaagtcat
     8221 tacttgcagc aacttctgac gcagaccagc cgatttgatc gataaacgct tgaggatcag
     8281 caactgtttg agatccttgt tcatctgcat gaacatgttg cggtaccgct gtaaccatca
     8341 tcggcccaac taaaagactc gttcc
//
LOCUS       DACDRN010000091         8249 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDRN010000091 DACDRN010000000
VERSION     DACDRN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.091"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            51..218
                     /locus_tag="IWJ80_002489"
     CDS             51..218
                     /locus_tag="IWJ80_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9462173.1"
                     /translation="MVYLNKKQTCEFLGIDNNTLDKWIRIYNFPVIKIDGIIRFDVED
                     IKGFMDTFKKI"
     gene            292..1518
                     /locus_tag="IWJ80_002490"
     CDS             292..1518
                     /locus_tag="IWJ80_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9462174.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFKEITGISSEKFQKTFPTRQLAIKA
                     ENEMKKKIAKVLREENANSLELKGQIKFKEFYKSKWLPRYELGQTTRSHKAPSDITII
                     NTKNLFRLHILPIFGEYAMSYLNANTEIISDELTKKSKEYANIKIIKCYVRSMFDIAE
                     VLNYIEFNRTTKIIQSITAPKKVALEEKRIREGKQALSSKELTDWLDAVRDDFNDQLL
                     SFHDYTLFMLTLYLGDRKSETYALQWKYIDFENQTVRLKYALDKHTKKKFTKGRKDTI
                     IQVPEIVMTLLQEWKSVQAEQLLKLKIKQTPDQYLFSYSKPSGEVNCPVHTDYLNYRI
                     NSIKKRHPHLVHLTPHKLRHTYATLARQGGADMNQISNALTHSGISTTKIYVNTPDLV
                     DKAVFEAFQRGLQKSN"
     gene            complement(1676..2293)
                     /locus_tag="IWJ80_002491"
     CDS             complement(1676..2293)
                     /locus_tag="IWJ80_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9462175.1"
                     /translation="MAKIGYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTLKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTGINRRTFRRYRMKHGITNEVREQKNKSFS"
     gene            complement(2976..3971)
                     /locus_tag="IWJ80_002492"
     CDS             complement(2976..3971)
                     /locus_tag="IWJ80_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9462176.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKNN"
     gene            complement(4066..4938)
                     /locus_tag="IWJ80_002493"
     CDS             complement(4066..4938)
                     /locus_tag="IWJ80_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9462177.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            complement(4951..6378)
                     /locus_tag="IWJ80_002494"
     CDS             complement(4951..6378)
                     /locus_tag="IWJ80_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP9462178.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVG
                     SGVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEVKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            complement(6432..7706)
                     /locus_tag="IWJ80_002495"
     CDS             complement(6432..7706)
                     /locus_tag="IWJ80_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9462179.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            8009..>8249
                     /locus_tag="IWJ80_002496"
     CDS             8009..>8249
                     /locus_tag="IWJ80_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9462180.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYI"
BASE COUNT         2756 a         1529 c         1186 g         2778 t
ORIGIN      
        1 tcaattcaaa aattttagtt catttataag gggatgattt tattaaatca atagtctatc
       61 taaacaaaaa acaaacctgt gagtttttag gaattgataa caatacttta gataaatgga
      121 ttcgtatata taattttccc gtaataaaaa tagacggtat aattcgcttt gatgtagaag
      181 atatcaaagg ctttatggat acgttcaaaa agatttgaat tttacttttg actgcatata
      241 ctaaaagtag cgaaatatac tgccttactt ttttattaag gagtgaggaa gttgcctacg
      301 aagctttcaa atggaaaata taaaactaat ttgagatatc caaaaaaatt taaagaaatt
      361 acaggtatca gttcagaaaa attccaaaaa acatttccaa ctaggcaatt ggctattaaa
      421 gccgaaaatg aaatgaaaaa gaaaatcgca aaagtcttaa gagaggagaa tgcaaattca
      481 ttagagctca aaggtcaaat aaaattcaag gagttttata aatctaaatg gcttcctaga
      541 tatgagttag ggcaaactac tagatcccat aaagctccaa gcgatataac catcattaat
      601 acaaagaatt tatttcgatt gcatattctg cctatatttg gtgagtatgc catgagctat
      661 cttaatgcaa ataccgaaat aatatcagat gaactcacaa aaaagtcgaa ggaatatgca
      721 aacataaaaa tcattaaatg ctatgttcgt tccatgtttg atattgcaga ggttctaaat
      781 tatattgaat ttaatcgtac aacaaaaata attcaaagta ttactgcacc taagaaagtt
      841 gctctggaag aaaaaagaat acgagaagga aaacaagctt tatcttcgaa agaactcact
      901 gattggttag acgctgtaag agatgatttc aatgatcaat tactttcctt tcacgattac
      961 actcttttta tgcttacttt atatcttggg gatagaaaga gtgagaccta tgctttgcaa
     1021 tggaaataca ttgattttga aaaccaaaca gtccgtctta aatatgcctt ggataaacac
     1081 acaaaaaaga aatttacaaa aggtcgaaaa gatactatta tccaagttcc ggaaattgta
     1141 atgacactat tgcaagaatg gaaatcagtg caagctgaac aactattgaa gttgaaaatt
     1201 aaacagacac ctgaccaata tctttttagt tactccaaac cttctggtga agtaaattgt
     1261 ccagtccata ctgattactt aaattatcga attaattcta taaaaaaaag acatccccat
     1321 ttggttcatt taactcctca taaacttcga catacttacg caactttagc tagacaaggt
     1381 ggggctgata tgaatcaaat ttctaacgct ttgacacatt caggtatttc aactacaaaa
     1441 atttatgtga atacacctga tttagttgat aaagctgtgt tcgaagcgtt tcagcgagga
     1501 ttacaaaaat ctaattaatg tgttctagaa tgtgttctaa atataaaaaa aagacacttt
     1561 ccaaataatt ggaaagcgtc ttgaacgttg atatataaag gatattaacg ttttgagaat
     1621 tgagatgctt tacgagcttt cttaagacct ggtttcttgc aaaattatat tagcctcaac
     1681 tgaaagattt atttttttgc tccctaactt cattagtaat accatgtttc attcgatatc
     1741 ttctaaacgt tctacgatta atccctgtta gctcttcaac ttctttatct gtagatccag
     1801 tcaaaaacaa attaaatgct aattgaagtc gcggatcatt ctcacgatat tttagcgtac
     1861 ctcccttgta tttccctttt gctttagcga gtgcaattcc ttgttgttgc gtttcgcgaa
     1921 tataccgacg ttcttcttcg gcctggtact tgtatagttc gataattaag ttattaagta
     1981 atcgacgtaa attatcatca gtgatacctg ttaacgctgg caaattcaaa acttccagtg
     2041 tagctttctt cagctgaatt aaattcatcg tttctgttag ctctttatta tttcgtccaa
     2101 gtcgttttaa ttctgtcaca acgacaatat caccttcacg tatgaatttc atcatttctt
     2161 gaaatccttc tcgattggta tctttcccac ttgctttatc actaaaaatt ttatcacact
     2221 ctgataataa ttctaattgt cggtctaaat tttgatctaa gctgcttact cgagcatacc
     2281 caattttcgc cataataaaa ctcctttaaa attctgaaat ccggatcatt tttaaattct
     2341 gttgcccaac tctgttgtca tttttctaga taaatggcat aaattttcag cgctaagtga
     2401 caaaagcaac taatttttca tgatagattt ttaaatatct caatctatct ccaaaattag
     2461 ttgcttttgt agctatagtt gactatgaat atgacaaata attagaaaaa actgtgccaa
     2521 ttaaatataa aaatgacact catttacatt caatagaaca ccaagagccc aacagggtat
     2581 actctggtgt ccttttaaaa aagtaacttt ctgacctaat taattgagct actaaaaaag
     2641 ctatgaaatg aggaataaaa aagggatcaa aaacttttaa gtttctgacc cttttgagtt
     2701 ctaattattt cttccagaac gaagaatgtt atctgcttta tctgcaattg attctgttag
     2761 aaggactgaa aaatttatgc cagccctttt gcctagctta tccgcccatt gaggaattgt
     2821 taaggttttt ctgacaagtt ctatgtcttt caaatatttg gcaacatcta cacagattaa
     2881 aagcatgatt ttgtatcata gtctattata agagatttaa caatttttta tatcacgaat
     2941 taataatgaa attatcattg atagtcttta taaatttaat tgttcttttt ctttactgat
     3001 gtcttcccca tccataatgt gacaggtaaa atatattcct tttttgtttc ttctttattt
     3061 attcgtttca tcaaaatagc aacagctgtg tctgctatat cattaattgg ttgtattact
     3121 gttgtcagat ctggtaatat actccgagtc attaaagtac catcatatcc gactactaat
     3181 aaatcagctg gtacatttaa ccccttttct tttgctactt gtaatattag ggccgcatcg
     3241 atgtcatttg aagcaaaaat accatctatt ttaggataat cctcaaacat ttggaaaaaa
     3301 atctgttttt tctttacata agaaatattg aaatctaatg tcacagttcg tggaattaac
     3361 tgatatgctt tcatagtatc ttcataggct agtctccgac gattagccgg agtctgtaag
     3421 tcaattggac cgtttgtatg aataatattt tttgcacctt gtgcaattaa taacttggtt
     3481 gcaagttttc caccattgta gttatcggat tcaacaactg gaatatcttc attcatcact
     3541 cgatcaattg caacaatcgg taaattctga tatttatatt cctgaattcc ttggttatgt
     3601 gtaccaacaa ttaaaccatc tacttgatca gacaataact gatttaaata atttgtttcc
     3661 ttttctttat tattcatcga attgcctatt aataccttat aaccaatttc atataacttt
     3721 ttttccaatg cctcaacgag ttcactaaaa aaaggattgg ctactgtagg aaatagtaac
     3781 ccgacaatgt tagttttctt ttgaaataat tgacgcgcta caacgttcgg ttgataattg
     3841 agttcttcta tagccttata aacattatca atcgtttttt gacttaaata gccacgattg
     3901 tttaatacac gtgaaacagt tgtttttgat acatttgctc ttttggcaac atcttctaat
     3961 tttgggcgca ttctcttgtc tctcctaaat aatgcaataa ttaactatcg aaaaaaagaa
     4021 ctctatagaa ctactatacc tgataatcga tattttttct atgttttatt cttgtaattg
     4081 attcaagccc aaagcaaagt ttcctagtgt tgccgatcca ttatttttaa ttaaaggacg
     4141 cgtaatatat ttacttaacg gtggtatttc cacgtaatca tccaataatt tattaacatt
     4201 aacacgaatt ttttctaaca atttttcatt tactacgctt ccaccaaata ctattttatc
     4261 cggtcgtata atcaatgttg tttgaatcac agcttgtgct agataatagg ccattacatc
     4321 ccaaatagga tccgttaaag aaacgtcttc accctttttt ccaaaacgtg cttcaaacgt
     4381 tggtcccgca actaagcctt ctaaacaatc accatgataa ggacaaattc ctttaaaatc
     4441 aatatcatca ggatggcgtt ttacaaacgt atggcccatt tctgaatgac caaacgatcc
     4501 taatagtttt ccgtcaacaa cagctccacc accaacgcca gtaccaacgg tgtaataaac
     4561 taatgaattt attttttcat tcgataaagt ggacatgatg tactctccat aagctgatcc
     4621 gttcacatcc gttgtccaag acattggaac attcagctca tcttgtaaat aacctaacac
     4681 attaacattt cgccatccca cctttggtgt agacgtaata tatcctgatt ttggtgaatt
     4741 ttttcgaatt tctattggac caaatgatgc aatacctaat gcatttatat caaactgttt
     4801 aaaatagtca acagtttttt tcaatgtttc cctgggggtg gtcgttggac aaattatttg
     4861 gtcttgtact cggtaatcct cgtctcccac ggcacataca aatttagtcc cacctgcttc
     4921 tatgctacca attaacatca taaaacctcc ttactcgtaa gaaattgcat ctttttttag
     4981 atgataaatt gtactcgaga caaaagcttc ttcactaaca ctcaaatgca tacttggttg
     5041 attttctgta taataacgct ctgtaaaaac aagctcgcca tcattaataa aaatctctgc
     5101 cgaactttta tcaataaaca ctcgaagtga taatctatca cacatctgaa tggttccata
     5161 ccttggatca tttttgccgt tacgagttaa ggtacattca tttttatcct tagaataaaa
     5221 aatattgatt aactcttcat cttttgtttt taatgaaaat ttgacttctt gattttttga
     5281 tttagtcaat agtacatcaa ttagagcttc tttgaatgaa gcatcttgag cgattattat
     5341 ttctcctgcc aaattaactg tcgtttcaac gccgctgcca actcgaagtt gttctaattc
     5401 tctcacaggt ttcatataca aacgatctcc aattaactct aattctcgag gaagcgttaa
     5461 tgctcctgcc catccgtcta ctttttcagg catagggctt tcccacatat ccatccaggc
     5521 cataacaata cgtctaccat ctggagataa agttgtttgt gttgcataga aatcgtgtcc
     5581 cttatctaat tcataaaaat ttcctctaat gaattttgct gtctcataat cataatcacc
     5641 gataaagtag cctgtttcat atagatttaa ataatctttt ccatttgctt caattccctg
     5701 aggggataat aaaagaatag attttccatt taattcaaaa aagtctgggc attcccacat
     5761 aaatccttct gtaagtactt ccttcgcttt agaaattggt ccaatgtaat cccaatttaa
     5821 taaatctaac gactgataaa caatagcacg acctaatcca tctttttctt gactacccaa
     5881 aaccatgtaa tatttttcat cgtgtttcca tacttttgga tctctgaaat gatgtgtgtt
     5941 atcttctggt gcttttgcta ttacaggatt tttttcatat ttagtaaagt gaatgccatc
     6001 cgtactatac gccatgtttt gattttgcca aaaatgatct ggatcattgt ctccataata
     6061 gtgatgtcct gtatagaaca gatacaacac accatttttt tcaatagcgc ttcctgaaaa
     6121 acaaccacct tcatcttctt gatctccagg agtcaaagca agtggtaaag attcccaatg
     6181 aactaaatcc ttacttctag catgtgccca gtgcattggt ccccattcag cactatatgg
     6241 atgatgttga taaaacacat gataataccc atcaaaatag caaaatccat ttggatcatt
     6301 aatccatcct gatggtgcag acacatgata tcccaaacga taacgtgtat ttgttaattc
     6361 tatagctctt tttgtcataa tatattcgct cactttctag ataaaattgt tttttattct
     6421 gttggtttcc attatcctcg aataaatgga tccccttgaa catctctatc atcttgttta
     6481 agaataaaga atgcatatat gcctgctagt aatacaataa gagaaataat tttaaatgtt
     6541 ggttgatagc caacgttgtc tcttaatatt cccaatggtg ttgataaaat cacttgccca
     6601 atttgagcag ctatctgaaa tcctatcata tagagggttg ctgatagctt tgtatcaaaa
     6661 tgaagtgtaa aatagcgaaa cattggtaat gtaaataatg gtacttctaa agcatgcaac
     6721 atttttatgc atgaaacaga taatggtgtc gaggcaaacc cgcacaatcc aattcgtaca
     6781 cacataatgg ttacacctaa taacaaagta tttcgaaccc ctaattttct cataatgatt
     6841 ggaacaattc ccatcattaa cgcttcacaa aatacttgaa tagcattcaa tgtcccatat
     6901 attttttcac cattagctga tgttgaaaac aaaccagtat aaaatcccgg aaacatttgt
     6961 tgatcgaata ccgtataaaa tgtccatgta aaaacaataa agatgattat cgtccataat
     7021 tgaggcagtt tcattagatc aaacatttct tttaaagaag gaatactatt tgattcagct
     7081 tgttcttgat taaaattttg atttgcttcc cgttcaactt tagggttcca aaataataaa
     7141 tttaacaata gtaaagaacc aaagaaagat cccgcccaaa agtttaaatg aggatttttt
     7201 acaaataaaa atcctgccaa aagcgctacc aaggcataac caaatgatcc ccacgccctt
     7261 gcttgtccat attcaaagcc aaataaacga ctaaaccttt ctgacacagc ttcaaaaact
     7321 cctacagaag ctaaatatcc agctgacaaa aatagcgctc ccataataat gcctaaatta
     7381 aaattgttat ctagcaatgg cccatatatc catataaaaa atggtccaac aagtgtcgct
     7441 aatacagaag caaaaattaa taaatttcgt ttaatataca atttatcttg taatgttcca
     7501 taaataaaca tcaaaattaa ggtaactaac gaatttgccg agaatactgt tcctacagca
     7561 ctgccagata accctaaacc atttgattca gatgttagcc aaagttgaaa aaatgaccac
     7621 caaacacccc aagaagcaaa aaatagtaat aatgtagctg aactttgtaa ataagattga
     7681 tttttaaata cgttaacttt tgacatattt ttatcctcca acgatatatt tgtatcccct
     7741 ttcatgataa ccggttaaca tttttagaaa aggacttgta ctcctaaaat tataaaagaa
     7801 atccgcttac atgtcaaccg gttattctat ttggataatg ccatataatg tcacaggata
     7861 tgacttttat tggtgtgtcg cttttctttt ttaatggcac tattttttat aacgccgttt
     7921 gtataattaa gcgagacaaa taaaaaagcc atttatgtta cactcaaatt gtaatacccg
     7981 ctaaagcata caaggagagt gaacataaat gacttatacc catcttacac caaacgagct
     8041 tgtaatgata gaagcatatt ttcatcaaga aactccggtt gctatcgttg cgaagcagct
     8101 taaacgtgga cgccaaacaa tttacaatgt ctataacttt ctcaaatgtg gtggaacagc
     8161 acttgaatac tttgaacaat acaaagaaaa taagcgacgt tgtgggagaa ccgaaatcat
     8221 ttttcctgct gaggaaaaag aatacattg
//
LOCUS       DACDRN010000092         8229 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDRN010000092 DACDRN010000000
VERSION     DACDRN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.092"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="IWJ80_002497"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="IWJ80_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP9462181.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="IWJ80_002498"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="IWJ80_002498"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP9462182.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="IWJ80_002499"
     CDS             2591..3376
                     /locus_tag="IWJ80_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9462183.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="IWJ80_002500"
     CDS             3389..4240
                     /locus_tag="IWJ80_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP9462184.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="IWJ80_002501"
     CDS             complement(4364..4816)
                     /locus_tag="IWJ80_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9462185.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="IWJ80_002502"
     CDS             complement(5281..5649)
                     /locus_tag="IWJ80_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP9462186.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="IWJ80_002503"
     CDS             5799..6281
                     /locus_tag="IWJ80_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP9462187.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="IWJ80_002504"
     CDS             6318..7328
                     /locus_tag="IWJ80_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP9462188.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="IWJ80_002505"
     CDS             complement(7443..8156)
                     /locus_tag="IWJ80_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462189.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2441 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtct
//
LOCUS       DACDRN010000093         8047 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDRN010000093 DACDRN010000000
VERSION     DACDRN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.093"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWJ80_002506"
     CDS             complement(99..1229)
                     /locus_tag="IWJ80_002506"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP9462190.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IWJ80_002507"
     CDS             complement(1878..3080)
                     /locus_tag="IWJ80_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9462191.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IWJ80_002508"
     CDS             complement(3140..3343)
                     /locus_tag="IWJ80_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9462192.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IWJ80_002509"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IWJ80_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IWJ80_002510"
     CDS             4880..5692
                     /locus_tag="IWJ80_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9462193.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6868)
                     /locus_tag="IWJ80_002511"
     CDS             complement(6716..6868)
                     /locus_tag="IWJ80_002511"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462194.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="IWJ80_002512"
     CDS             complement(7059..7937)
                     /locus_tag="IWJ80_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9462195.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
BASE COUNT         2458 a         1647 c         1254 g         2688 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagta
//
LOCUS       DACDRN010000094         8047 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDRN010000094 DACDRN010000000
VERSION     DACDRN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.094"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="IWJ80_002513"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="IWJ80_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP9462196.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="IWJ80_002514"
     CDS             complement(2203..4389)
                     /locus_tag="IWJ80_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP9462197.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IWJ80_002515"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IWJ80_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP9462198.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="IWJ80_002516"
     CDS             complement(4611..5051)
                     /locus_tag="IWJ80_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP9462199.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="IWJ80_002517"
     CDS             complement(5125..5664)
                     /locus_tag="IWJ80_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAP9462200.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6279..7745)
                     /locus_tag="IWJ80_002518"
     CDS             complement(6279..7745)
                     /locus_tag="IWJ80_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9462201.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2197 a         1754 c         1388 g         2708 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgaggggc tatttttgtt tggctgttct tattacttga tgcaggacag
     6121 ctttttcaca acctctgatt cacggtgttc cagaagtagc ttctgcgaaa aacaagccga
     6181 aacttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac
     6241 cacttctgtc acaacctctt gtaacaagta tttctatttt aaggctgaac cactgtttct
     6301 ggttcagtga tttctttgtt tttgtctcga cgagcaattg ctggcaagat catacctaat
     6361 aagatcaaga caattggtgt gatgatattc gtgaataatt ggaaccacca tgctttagga
     6421 tcagcagtat aatctacttt tggcaccatt cctaggatac atgcaaacgc agtgaaggca
     6481 aagcaccaag caccaaagat aaaaccgatt tttggatttt taacaaattt atattcagaa
     6541 ttgaatttct tataagcttt attcaacatc atatatgcca agaacaccca taggtaacgc
     6601 attggcatga caactgagtt caagttcgtc aaccatttta ctaattcatt catatctttg
     6661 ataccaaaga tcggcaacaa aataatgatt cctactagta tacctgtcag aagataacca
     6721 ttcaccaatg tacctttacg attacgttta cgtagccagt ttggtacaaa ttctggatcc
     6781 gcatctgcta acaagatttt tagtggtgca tcaattgaaa aggctaatgc ggagatttgt
     6841 ccaatcatgt ttgtcaatgc gtaaatgatc atcaatgagt taccgatacc atagtaatta
     6901 cctaaaattt gaaaagcact gtatgcccca tttgccatca agtctttagg aatattatcg
     6961 ctagcaaaca gcatacccat tgcgactgag cctaatacag cacaaatccc taccattgct
     7021 gctaagaaga tcatcccttt aggaaattcc tttgcaggat ttcttgtaga gttgacataa
     7081 ggggatattt tttccgctcc tccaactgca aatactagca tcgaaacggt tgtgaaataa
     7141 ctgaaatcaa atgttggaat atatgttttc acttgattca tatgtggtgt ggcaaattcc
     7201 atcgttggat tgatgaacgg tgccgttaca gctaaaagaa taaataagat cgacatgacg
     7261 aacatcgctg ttccggccag tccgccaatc actttcaaag tagaaagtcc ttttgttgat
     7321 aaatataaaa aggcaagaaa tataataagt gaaataactg ctaccatttg tactgacatc
     7381 gtatttacga tatttccatt tccttggact gcccaaccgc cagcaatcag gattgcttga
     7441 ggcttttgtg ccaaataagg aatatggacg acccagtaag tccaagcggc atagtatgcc
     7501 agtcgtttat tagatgtcgt attttcgacc catgaactga cgcctccttt actatccttg
     7561 aaggctgacc ctaattgtcc aacgatcaat gcgtatggaa taaaataaat ggccaaaatc
     7621 aatagccaag aggtgacaac tgatactcct tgctgagcat agttattaac tacattcccc
     7681 atgccccaaa ccatgttaaa tgcaattaac gcaacagtaa accagcgtaa ttgtttgttg
     7741 ttcattcgtt ctctccttta tgataaaaat ttgttatcca gatgtaatca tattcaattt
     7801 ttaacaaact tacaagacag tgttatttta tgtgtgtatt cttttattta atacacatcg
     7861 ttttttcata ttttttccat caaaaaaata agttattttc ttttatttca tttaccttat
     7921 tgaatttcaa gctattttga attttctgca aaattaaaaa cattagttat tttataatcc
     7981 tatctatttt ctttacattt ttcattaaga aaaggatata aaaaaaagag gacgttcatt
     8041 cgtcctc
//
LOCUS       DACDRN010000095         7924 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDRN010000095 DACDRN010000000
VERSION     DACDRN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.095"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            4..1458
                     /locus_tag="IWJ80_002519"
     CDS             4..1458
                     /locus_tag="IWJ80_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HAP9462202.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
     gene            1534..2694
                     /locus_tag="IWJ80_002520"
     CDS             1534..2694
                     /locus_tag="IWJ80_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAP9462203.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            2807..3814
                     /locus_tag="IWJ80_002521"
     CDS             2807..3814
                     /locus_tag="IWJ80_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9462204.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            3838..4863
                     /gene="iolG"
                     /locus_tag="IWJ80_002522"
     CDS             3838..4863
                     /gene="iolG"
                     /locus_tag="IWJ80_002522"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HAP9462205.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            4880..5770
                     /gene="iolE"
                     /locus_tag="IWJ80_002523"
     CDS             4880..5770
                     /gene="iolE"
                     /locus_tag="IWJ80_002523"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HAP9462206.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            5793..7388
                     /locus_tag="IWJ80_002524"
     CDS             5793..7388
                     /locus_tag="IWJ80_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAP9462207.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            7404..7718
                     /locus_tag="IWJ80_002525"
     CDS             7404..7718
                     /locus_tag="IWJ80_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9462208.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
BASE COUNT         2356 a         1505 c         1919 g         2144 t
ORIGIN      
        1 taaatgacaa gatttttaag aaattatatt gatggtcgtt gggtcgctag tcgctcaaca
       61 gagtttgaag aagtaatgaa tccggctaca gaagaagcgc tggcgaatgt accgttgtct
      121 agcaaagcag agacagccga agcagttaaa gctgccgatg ctgcgtttca aacttggaaa
      181 aaagttcctg ttcctgttcg cggacgttat ctgttcaagc tgcatcagct tttaacagat
      241 cataaaagtg aattggcgcg tttgatcacg ctggaaaatg ggaaatcatt gtccgagtca
      301 ctgggagaag ttcaacgagg aatcgaaaat gtggagcacg cagcgggaat caccaattta
      361 atgatgggag attctttatc aacagcggcg actgatattg agatcacgaa ttatcgttac
      421 ccaatcggag taatcggagg gatcacaccg tttaattttc caatgatggt gcccttctgg
      481 atcttcccga tggctctggc ggcaggaaat acggtggtgc tgaagccttc agaaaaaaca
      541 ccattgttga tggagaaaat tgttgagctg gttgaaaaag caggctttcc tagcggtgtt
      601 tttaatgttg tctatggtgc ccacgatgtt gtcaacacat tgctgcaaga tccactggta
      661 aaaggcatca gctttgtagg atcaaaaaat gtcggtgaat acgtttaccg tgaaggaaca
      721 aaaaatctaa aacgggtaca agcgttgact ggagcgaaaa atcatgttgt tgtgctgaaa
      781 gatgccaata ttgatcgggc agtcaaggac cttattggcg gagcttttgg ttctgctggt
      841 gaacgctgca tggcagcctc cgtcattgtt ttggaagagg aaatcgcgga tcagtttgtt
      901 gaaaaattta aggctgcggc aaaagcgatc aagatcggca atggcttgga cgaaggggtc
      961 tttttaggac cagtgattcg aaaagatgca cagcagcgga cctttgacta tatccaaaaa
     1021 ggggtcgatg aaggagcgaa actggtacta gatggtcgcg aaaacattcc agaaaaaggc
     1081 tattttgttg gaccaacgat ttttgataat gtcacgatgg atatgaagat ctggcaggat
     1141 gagatttttg ccccggtcgc atcaatcgtg cgggtaaaag atttgatggc agccgttgag
     1201 ctgacgaatg aatctgaatt tgcgaatggt tcgtgtctgt ataccaacaa cgcatcagcg
     1261 attcgctact tcagagaaaa tgttgatgct gggatgctgg gggtcaatct tggggtgcct
     1321 gcaccgatgg cttggtttgc cttttcaggc tggaagcact ctttctttgg tgatttgcat
     1381 gccaatggca aagacagtgt tgagttttat acccatcgga aagttgttac agcacgttat
     1441 gcgcaaagtg agatttaaag atttgcttcg aagctctgtt aaagagcttc gaggattctg
     1501 ttataggcta taagccagag agaggttttt tctgtgaata ttcaactgaa aatgcctgcc
     1561 aatttaagaa ttggcggcgg aacaatccaa tttcttatgg aagaatcaga acaactaggc
     1621 aacaagccgt taatcattag tgatacggtt atggaacaga ccgggcatct tcaaaagatc
     1681 aaggagctgt tgggaaaaac agatgatcaa ttagcggagt atacagacat tcaagccgaa
     1741 ccgacgcaag aaaatgttta tgatgcgctg aagctttttc agaagcatca atgtgatcat
     1801 atcattgctt ttggtggcgg cagctgtctg gatacagcca aggtggtagc cgttttggct
     1861 gctaatcaat taagtctcga agcgattcat gcaagaaatt ttgatcagct gcaagcgggc
     1921 attccgctga ttgcagtgtc aacaactgct ggaactggtt cggaagtaac cgatgtggca
     1981 gttattacca atacaaaaac agcagtcaaa atgatgatga aggatctcgt tttcatgcca
     2041 cagatctcaa tcgttgactc tgattttacg aaaactgtac caccaaaagt aaccgcggca
     2101 acgggagtcg atgcgctatg tcacgctttg gaatcttatg tttcagtcaa agccaatgaa
     2161 gtaacgcaac tattttcact ggaagcgatg aagaaaattt tgaacacgct ttctcaggca
     2221 tatgaaaata gcgacgacac tgaagccaga gaacaaatga gcttagcttc aacggaggcg
     2281 gggattgcct tttcgaatgc gtcagtcaca ttgattcatg gaatgagtcg accggtgggg
     2341 gcactttttc acattcctca tgggatatca aatgcgatgt tattgccggt cttttttgaa
     2401 ttctcaaagg atgctattca gactgagtta gcgacgatcg ccagatttat ttatccggca
     2461 gccgaagatt tatcgctgga agaggctgcg gattatctga ttaaaaaaat ccaagagctc
     2521 tgtgagcagc tgtcgatccc aacgattcga gattatggta ttgatcagca agcgtttatg
     2581 gcagccttag acaaaatggc cgcggatgcg ttgaccagcg gaagtccgca aaataataaa
     2641 aaggtgccta gtcatagtca aattaaggaa ttatacgtac aagcttatgg gtagtaaaag
     2701 gcggagcaaa gagagggctc taccttttaa gaataaaagc tacacaggaa taattttgta
     2761 agcgcatact atgtgtgctt aaacaacaga acagggggat aagtaattga gagtaaaagt
     2821 aggcgttatc ggaacaggtg caatcggcat cgagcatatc gacaggatca atcagaaaac
     2881 acaaggggct tatgtgacag cagtctcaga tatcaatgta aaagctgcta aagaggttgc
     2941 caaacgtgtc gatgctaaat tctttgaaac cggagaagca ttgatcgctt ctgaagatgt
     3001 ggatgtcgtt gttgtgactt cttgggaccc gacccatgag cagtatgtgt tagaagcaat
     3061 caagcatggg aaatatgttt tctgcgaaaa gccattagcg atggatgcag caggctgccg
     3121 ccggatcgtt gatgcggaag ttgcttatgg gaagaaattg gttcaggttg gcttcatgcg
     3181 ccgctatgac cgcggctaca ttgaactgaa agaagcaatc gaatcgaagc aatacggaga
     3241 agcattaatg atgcattgtg cccatagaaa tcctagcgca gatgaaaact acacaacacc
     3301 gatggcgatc agcaacaccg cgattcatga gattgatgtg cttcgctggt tgctaaatga
     3361 agattatgaa tctgttcaaa tgatcttgcc gaaaaaaacc agcaactccc atgccgattt
     3421 gcatgatccg cagctgctga tctttcaaac aaaatccggt gtaacgattg atttggaaat
     3481 ctttgtgaat tgccgctttg gctacgatat taaatgtgat gtggttttcg aaacggcaga
     3541 agtaggaatg acggacccag cctatacacg aattaaatcg gaagaaaaaa attacacacc
     3601 tgtttcacca gattggcaga cccgcttttt ggatgcctat gattatgaat tcaagctgtg
     3661 gatcgattcg atcaaaaatg atgcggttgt cggtcctacc gcttgggatg ggtatgtagc
     3721 agcgatcacc atgatggctt gtcatgagtc tcgtgaaaca aaaacaaaag taatgattca
     3781 aatggatgaa cgaccagcac tatacaatga gtaattctca gaaaacggag gtatttcatg
     3841 aacaacatta gagtaggcat cgtcggttta ggaagattag gaaaagtaca tgcacaaaat
     3901 ttagcaacag gtgtcagttg ttgtgagctt acggctgcgt gcagtttagt tgaaacagaa
     3961 ttagcattcg cacagaagaa attaggcgtc aagcatactt tttccagcta tgaagaaatg
     4021 gtcaaaagtt ccgtgatcga tgcagtcttt attgtttcac catcaggctt tcactgtgac
     4081 caagtccgct tggcgatgga aaatggcaag catgtcttta ctgaaaaacc attaggcctt
     4141 gaagttgaag atattaaaaa gacccaaaaa gtcatcgaag agcatcctga acaggtcttc
     4201 atgttaggct tcatgcgccg ctatgatgag tcgtatcagt atgcgaagaa aatggtagaa
     4261 gatggcgaaa tcggcgaaat cacactgatt cgttgttatg ggatcgatcc gagcgaaggc
     4321 ttggaaagct ttgtcaaatt tgccatgaat aacgacagcg gcggcttgtt cttagatatg
     4381 gcgatccatg atattgattt ggttcgctgg tttaccggcc aagaagtcga tcaagtgtgg
     4441 gcgattggga aaaatgcggc gtatccagaa ttggatcagg ctggtgagct tgaaactgga
     4501 gccgcaatga tgagcctgag cgataaaaca atagctttac tagttgccgg cagaaacgca
     4561 gcccatggct atcatgtcga aacagagatc atgggaacca aaggaatgct gcgggtagcg
     4621 caagcgcctg agaaaaatct tgtgacgatc atgaacgatc aagggatcat ccgtccatgt
     4681 tcacaaaact tcccagaacg attcagagaa gccttcattc gtgaagccca agaatttgtc
     4741 tcatgtattc aagaaaaacg tcaaccagcg gttaatgcag aagatggatt gcaatcaact
     4801 aagatcgcgt tggcttgtca agaatcattc gatacgaaga gtttagtaca actaaaaaaa
     4861 taagcaggag tgtgagaata tgaccaaaca aattcaatta gccatcgcac caatcgcgtg
     4921 gaccaatgat gatatgcccg agcttggtag tgaaaatacc tttgaacaat gtatcagtga
     4981 aatggccctt gctgggttca ccggaacaga gattggcaac aaatatccaa aagatccaga
     5041 ggtgttggaa caatatttga atattcgcgg cttaaaggtt gccagtgcat ggttcagtgc
     5101 gtttctgaca actaaacctt ttgaagaaac tgccgaagcc ttcaaagcac atcgggattt
     5161 cttacatgcg atgggcgcta aagtcatcgt agtagctgag cagggtcata gtatccaagg
     5221 gattatgaca gcgccagtct ttgatgataa accagttttc actgatgaag agtggaaaaa
     5281 actggcggaa ggattagaaa aattagggga tttggcccac gaaaaggata tggagatcgt
     5341 ttaccatcat cacatgggta ccggcgttca aacgacggaa gaaatcgatc gtttaatgga
     5401 aatgactgat ccagacaaag ttaaattatt gttcgatacg ggacatttgg tcttttctgg
     5461 tgaaaatcca atcgcggttt acaaccgtca tcaagatcgt atcaaacaca tccatttcaa
     5521 agatattcgt caagaaacgg ctgaagaagt agaaaaggaa aaagacagct tcttaaacgg
     5581 cgtgaagaaa ggcgtcttca cggttcctgg tgatgggatg attgatttca ctccaatctg
     5641 gacagcaatc catgccagcg actatgaagg ctggatcgta gttgaagcag aacaggaccc
     5701 ggctaaagcc aatccatttg aatatgcagt caaagccaga agctacattc gagaggtcac
     5761 cggtctttaa tctattgaaa aggagctaga cgatgaatct tttttctata atcagtttcc
     5821 tagtgattgt catctgtgta tggttatttg cctattctag atccagaagt gtcgatactt
     5881 ctggatcaga agggttcttc atgggagggc ggagcttaac tgctttgcca atcgccggca
     5941 cgatcatcat gacgaacctg tccaccgaac aaatagtcgg acaaaatggt caaagttatc
     6001 atgcagggat ggaagtaatg gcctgggaag taactgctac gattgcgatc gtagctttgg
     6061 ctgtgatatt cttgccaaaa tattttaaat acggcatcaa tacaatctca gatttcatcg
     6121 agattcgtta tgatacggtt accaaacgaa ttatttcgat cttatttatt gtgacctata
     6181 tgacttcttt cttacccgtg gttctttatt ctggatcgct ggtgttcaat aagatcttcc
     6241 atattgatga gctgctgggc gtccagccga tcgtcgcgat cataattgta gcgatggcaa
     6301 ttggtattgt cggcatcctc tatctgctga tcggcggctt gtcgttaagt gccaatagtg
     6361 actccattta tggagtcggt ctgctggctt gtggtctgct ggtcccaatt ttaggcttaa
     6421 tgaagcttgg tgacggcagc tttgttggcg ggatcgaaca cgttgtggta aacacgccat
     6481 ggctgcttaa ctctgtggga tcgatcgact cggctgttgt tccatggccg acactattta
     6541 cagggatgct gtttaacaat ctgtatttct ggtgtaccaa tcaaatgatc gtccaaaaag
     6601 cattgtccgg caagaattta gctgaggcac aaaagggcgc tttcctagtc ggcgtcttca
     6661 aaatcttagg tgcgttattc cttgtctttc cagggatcgt cgctcgcaat atttttggcg
     6721 atagtttaat gtcaaatcca gataacgcgt atcctttttt agtcagtgaa gtattgccta
     6781 aaatgatctt tggtattttc gccgctgtta tctttggcgc gatcttatca tcatttgcgg
     6841 gagctttgaa cgcgacatcg accttgtttt cactggattt ttataaaccg cttatcaata
     6901 agaaagcaaa tgataagcaa atcgctcgtg ctggtaaaac agtaacggtc gttgtgggga
     6961 ttatctccat tatcgtagcg ccattcattt cttttgcacc ggctggattg tatcagttcg
     7021 ttcaagaatt caatggacta tacaacatgc cgctgctcgt gatcattctc tttgcctttt
     7081 attcaaaaaa agcaactgct tttggggcaa aaacgacgat tgccgtgcat atcgtcttgt
     7141 atgcactatc aaaaatgctt ttccaaaata tccatttcct ttatgttttg acggcattat
     7201 tcgttctgga cattatcatt ttgatggtgg tttccaaatt gaaacctgat ggagaatttg
     7261 tgttgaactc attcgataca aaggtcgata tcgagccttg gaaatacacc aaggtgactg
     7321 ctatagcatt ggtagtcatt gttattttaa cttatgtggc attttcacca attggctttg
     7381 caggctaaaa acaggagggt attatgcata aggatctatt gattatttgc gagaccggaa
     7441 tcagtgcgtc gctgctcgta tcaaaaatgc ttgaaacgat cagagccaaa aatctatcgt
     7501 atgagataga ttatgcaacg atccgaaacg ttgaagaaaa actgaattat aaagactatg
     7561 acgttttact tttaacgccg caggtcgcac gttctgagga aaaagtaaaa gaaatcatca
     7621 aaaaggaaaa atgtaccgct cagatcgttt atatcaaaca agaagatttt cgttatatga
     7681 atattgaaaa catcattgca agcatcgaag aagattaacg agaatctttg attattgagt
     7741 gtcgaggagg tggtatatga aaagatggca tgttcgcccc gatcacccca atatgaaggg
     7801 cggacttatt agaaataccc aatcttgtat ttattcaata gggtaagcgg acctcaatag
     7861 ccagctcatc cccaaaaagt cgaatggaga cacttaaatg agtgtaccta ttcgactttt
     7921 tgag
//
LOCUS       DACDRN010000096         7614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDRN010000096 DACDRN010000000
VERSION     DACDRN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.096"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(14..706)
                     /locus_tag="IWJ80_002526"
     CDS             complement(14..706)
                     /locus_tag="IWJ80_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9462209.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IWJ80_002527"
                     /pseudo
     CDS             791..967
                     /locus_tag="IWJ80_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IWJ80_002528"
     CDS             complement(1143..1712)
                     /locus_tag="IWJ80_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9462210.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IWJ80_002529"
     CDS             complement(1786..2109)
                     /locus_tag="IWJ80_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462211.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IWJ80_002530"
     CDS             complement(2263..3075)
                     /locus_tag="IWJ80_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462212.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IWJ80_002531"
     CDS             3429..4277
                     /locus_tag="IWJ80_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP9462213.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IWJ80_002532"
     CDS             4422..5363
                     /locus_tag="IWJ80_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP9462214.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IWJ80_002533"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IWJ80_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2269 a         1508 c         1370 g         2467 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttt
//
LOCUS       DACDRN010000097         7610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDRN010000097 DACDRN010000000
VERSION     DACDRN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.097"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..183)
                     /locus_tag="IWJ80_002534"
     CDS             complement(<1..183)
                     /locus_tag="IWJ80_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9462215.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIV"
     gene            308..3295
                     /locus_tag="IWJ80_002535"
     CDS             308..3295
                     /locus_tag="IWJ80_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAP9462216.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLL
                     EQLDLPSSANEMSLWLKNDLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKE
                     DKSFRRRIQQLLPKIDLSDLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSE
                     SCNIGFSPVSKEGFDSLKYDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDG
                     HMASADGIRYITPQRSLYSRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSL
                     YLLEGFLNQPSELTPNQIMTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDAN
                     AHYGELDTLSQNSINMELIYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGK
                     AITEYGKVYKTKHQLRYISDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYD
                     GMEEQLNALGLVTNAIIYWNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVG
                     KYTFRYEAELSNGKLRPLNVEEF"
     gene            3444..3653
                     /locus_tag="IWJ80_002536"
                     /pseudo
     CDS             3444..3653
                     /locus_tag="IWJ80_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346508.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3788..3964
                     /locus_tag="IWJ80_002537"
                     /pseudo
     CDS             3788..3964
                     /locus_tag="IWJ80_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            3946..4302
                     /locus_tag="IWJ80_002538"
     CDS             3946..4302
                     /locus_tag="IWJ80_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9462217.1"
                     /translation="MWQTTLSNFDFKPLGLAIKEARLSKGLTREQVGSMIQIAPRYLT
                     NIENKGQQPSFHVVYDLVTLLNISLDGFILGEENSHKSSKRLQVEAQLDCLDEDELVT
                     IGKVIQAIYGEKCKFN"
     gene            complement(4816..4983)
                     /locus_tag="IWJ80_002539"
     CDS             complement(4816..4983)
                     /locus_tag="IWJ80_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9462218.1"
                     /translation="MCPICGNKTRLKMREDTELKNFPLFCPKCRQEILIEITKFRITV
                     ITEPDAKTQSR"
     gene            complement(5071..7011)
                     /gene="tet(S)"
                     /locus_tag="IWJ80_002540"
     CDS             complement(5071..7011)
                     /gene="tet(S)"
                     /locus_tag="IWJ80_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(S)"
                     /protein_id="HAP9462219.1"
                     /translation="MEEIKLKIINIGILAHVDAGKTTLTESLLYSSGAIKELGSVDSG
                     TTKTDTMFLERQRGITIQTAITSFQRENVKVNIVDTPGHMDFLADVYRSLSVLDGAIL
                     LISAKDGVQSQTRILFHALRKMNIPIIFFINKIDQNGINLPDVYQDIKDKLSDDIIIK
                     QTVNLNLKPYVIDYTEPEQWETVIVGNDYLLEKYTIGKTLNIAELEKEENERIQSCSL
                     YPVYHGSAKNNIGIKQLIEVITSKLFSPTQLNSDKLCGNVFKVEYSDDGQRLVYVRLY
                     SGTLHLRDSVNISEKEKIKVTEMYTSINGELRQIDKAEPGEIIILKNELLKLNNVLGD
                     KKRLPHREILENPLPMLQTTIEPCKSVQREKLLDALFEISDSDPLLQYYVDTVTHEIV
                     LSFLGEVQMEVTCTLIQEKYHIEIETRKPTVIYMERPLKKSEFTIDIEVPPNPFWASI
                     GLSVTPLPLGSGIQYESLVSLGYLNQSFQNAVMEGIRYGCEQGLYGWKLTDCKICFKY
                     GLYYSPVSTPADFRMLAPIVLEQAFRKSGTELLEPYLSFEIYVPQEYLSRAYNDASKY
                     CANILNTKLKGNEVILIGEIPARCIQEYRNSLTFFTNGRSVCLTELKGYQVTNIKSAF
                     QPRRPNNRIDKVRHMFNKINLH"
     gene            complement(7012..7098)
                     /locus_tag="IWJ80_002541"
                     /pseudo
     CDS             complement(7012..7098)
                     /locus_tag="IWJ80_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance protein"
BASE COUNT         2510 a         1286 c         1269 g         2545 t
ORIGIN      
        1 aacgatgacg caatcacctt cacggatata atttagtaat ttttgaaact caggacgctc
       61 agtatcttta ccactggctt tttcttggaa taatttatca atgttaagtc cttccatcat
      121 tgccaattgt cgatccaaat tttgatcttg tgaagatact cgaatatagc caatttttcc
      181 cataaaaaac tcctttttaa aagtgatata atttacatgt aaaatttact tgtagtttta
      241 ctgctatcat agcattttta aaaataaatt ttatgtagac ttaaaattta cttcatggag
      301 ggtttaaatg gatgaaatca aaagtaacaa aaaagcattt ggcatttatt taggagaacc
      361 tagtacagaa caattaaacc aattttttta tttgcatgaa aaagatttag aattgatgga
      421 gactatacga cgaccagcta ctagattagg ttttgcacta cagttagtct cactacgttt
      481 tttaggaact tttatattag atcttaccaa tattccccaa aatgtattga gttatgtagc
      541 aactcaacta aatattgatc ctggagaaat tatctactat agcaggagac agaccaggta
      601 tcagcacaca aaattgataa aatcacatta tggatatcaa gatttcaacc aaccggaaat
      661 agaaacatac ctagctaact ggcttttaaa tagagcgctg tatacaacag aatcttcaga
      721 tatgttgttt gatatgcttc taaaaaaatg tctggatgaa aagattgttt tacctggaat
      781 tactactttt tctcgttttg ttgctagtgc aattgaaaaa gctgaagagc atttataccg
      841 acaattgtct ttggttccaa ctgaagatga gagaaagcgg cttctcaatc atttagaatt
      901 aattggaact ccagtttatg gggcaataat caaaatggac attttgagaa ccccgttagc
      961 tgacgatagt cataaagaaa tagccagagg ttttgaccga ctaaagcaat ttcaagcttt
     1021 ctctactgaa aaatggaaga tggaatctat tcctgaaggg aaaataaaag ttcttgcaaa
     1081 ttatgcattt aaagctaaag ctcaattaat tcaacgaatg agtgaacaga aaaaactggc
     1141 gttgttagtg gcatttattt tcatatacaa acgtaaggct atggatgaac aaattttagc
     1201 actatctaat ttttttgaaa ctgtttttag acgcgccaaa aataaggaat ctaaagaacg
     1261 tcttcgtacc ataaaagatc tagatcgagc cgcatcgaca ctttcagata ttgtagattt
     1321 agtaatgtcc agtcattctg atactaatga actatccctt cgcgatcgta tcttagaaaa
     1381 atattctgag gaaaatgtta ccgaagctgt atcgcaagtt cggctattgg ttaaaaatga
     1441 tcgtgaacca atagccattt ctgaacttct gaacacttat cgaaagttca gaaaatttat
     1501 tccggatatt ttaactacac taacatttga cagcaatgac tacggtaaca actgtaagga
     1561 actctggacc ttcattaata atagatttcc taagccgatt acttacaaaa tgtttggagc
     1621 tattaaacat actcttccaa agaaatggat ttattacatt catgaatacc ctgaacaaac
     1681 taataaatgt gtattgattg cagctgtcga attgttaatt caatctttaa aacgccatga
     1741 tatttatatt cctcagagtg tcttatataa caatcctatg gattgcttga ttgatgagaa
     1801 aacttgggtt caacaaaaat acgttttatt agaacaacta gatcttcctt cttcagcaaa
     1861 tgaaatgtct ttatggttaa agaacgattt ggaactgtct tataaagaag cgctaaatcg
     1921 ttggcctact tctaaaatgg ccagaataga aaatgcagat cgcctggttg tctctagact
     1981 taaaaagaca tatgaaaaaa aggaagataa gtcttttaga cgtcgcattc agcagttact
     2041 tcctaaaatt gatttgtcag atttgttatt ggaagtaaac caacagttaa atcttactca
     2101 atcatttaac cacttaagtg acaaggacac caaaatgaaa gacttggata ttagtttatt
     2161 agcagttctt ttatcagagt cctgcaatat tggtttttcc ccggtttcaa aagaaggatt
     2221 tgattcttta aagtatgatc gtcttgtcta tgtcaatcat cattatattc gagtggatac
     2281 actttcagga gctaatcaaa aaataataca tgcacacagg aaacttgcca cctctaagat
     2341 atggggtgat gggcatatgg cttcagctga cggtattcgt tatatcacac cacaacgttc
     2401 cctatattct agaagtaatc caaaatattt tggccgtggt cgtgggatca cattctacaa
     2461 ttttgtctct gatcaatata tagggttcca cggaatggtc gtttcaggga cactgcgaga
     2521 ttcgctttac cttctagaag gattcctcaa tcaacccagc gaattaactc ctaatcagat
     2581 catgactgat acagctggtt atagcgatat gatttttggt ctctttggct tattagggtt
     2641 tcaatttagt ccaagaattg ccaatgataa aggaaccaaa ctttggcgaa tcgacgctaa
     2701 cgctcactac ggggaacttg atacgttgtc tcaaaattca attaatatgg aactgattta
     2761 tacccattgg gaggacattc ttagggttgc aggttcgttg aaatctggaa aagtgaatgc
     2821 aacagaatta acgaaatctc ttcaacggaa tgggcaaccc acgcctttag gaaaagcaat
     2881 aactgagtat ggaaaagttt ataagacgaa acatcagctt cgttatattt ctgacgaaat
     2941 atatgcacgg caaattttag aacaattgaa taagggggaa gctcggcatg ccttatgccg
     3001 taatattttt tatggacgaa aaggaaaact ctaccagact tattatgatg ggatggaaga
     3061 gcagctcaat gcattaggat tagttactaa tgccatcata tattggaact cactgtattt
     3121 agaaaaagta tttgaacaaa tgaaagctga aggattcgac tgttccgaag aaatgatacg
     3181 caggcaatct ccactaatta gtgatcacat caattttgtt ggtaagtata cgtttagata
     3241 cgaagctgaa ttatccaacg gtaagttacg tccattgaat gtagaagaat tctagcaatg
     3301 ttcgggtttt gcatgctacc gcactatttc gtatgactgt tccagcgaat cacttcgaat
     3361 agaaaaagag aaaaacacga gaacagcgtt aaatcaacgt ttcttgtgtt tttacttttg
     3421 tgctaccgca ctatttcgtt tctatgttcc atgtacccct ttatcaagaa gcgcaaacac
     3481 gccaaattgc cgattggttg atcggcatga acgcaagccc tttatatacc ttgaatttac
     3541 aacaaaaagg cgtacaaggt acattttcat taggacgtgt acaaacaccg actttatatc
     3601 tcatttatca acgccaggaa gcaatagaaa actttaaaaa agaacctttt ttcctgaata
     3661 attcatagtt ttactaatag tctttcctaa cattgattta atagtcttta aagggttctg
     3721 ttgcaaagtt ttaaatctac tatcaaataa ggtagaataa tagaaaaaga tagcaggagg
     3781 aatgacgatg aatcatttta aaggaaagca atttcagcag gatgtgatta ttgtagccgt
     3841 gggctactat cttcgttata accttagcta tcgtgaagtt caagaaatct tatatgatcg
     3901 tggcattaac gtttctcata cgacgattta tcgttgggtg caagaatatg gcaaactact
     3961 ctatcaaatt ttgatttcaa accattgggt cttgcgatta aagaagcccg tcttagtaaa
     4021 ggattgactc gtgagcaggt aggatcaatg atccagattg cgccacgcta tttgacgaac
     4081 attgaaaata aaggacaaca gccaagtttt catgttgtat atgatttagt gactttgcta
     4141 aatatttctt tggatggatt tattttaggg gaagaaaaca gtcataaaag ctccaaacgt
     4201 cttcaagtag aagcacagtt ggattgtctg gatgaagatg aattagtgac gattgggaaa
     4261 gtgattcaag caatttacgg agaaaaatgt aaatttaact agttcaataa tacacaatgg
     4321 attaattaat actatcagga aagattgatt acaaagatgt aatgaatttt ttctgatttt
     4381 tgttaatcat aaatagaaaa tgaatttatc tttgttacac ttaggaacta gagcgaatga
     4441 taaatagttc tttggttact cttatcgctg caaagtacag ttcatttttt gttgttggga
     4501 aaattttctg gatttattgc atatcacttc atccgactaa aaatttttga ttcccatagc
     4561 gaagttcgtt tttgttcctt aggaaaaact tgactgatat actagttttg aaatgacgag
     4621 taactctgta cttcatcaat aatcaagtat tgaattttaa ttgtatactt tttatgcttg
     4681 agggatgtct actgtaatac agctactgca aaaaaaatca gtacacttat tttcttataa
     4741 aggatatcaa gaactaagct caatggtatg catagaaaag aaaaagccga taaaatgaga
     4801 ttttgatatc tcgaattatc ggctctgcgt ctttgcgtct ggctctgtaa ttacagttat
     4861 tctaaacttc gttatttcaa ttaaaatttc ttgtctgcat ttaggacaaa atagagggaa
     4921 attttttaat tcagtatctt ccctcatttt taaacgggtc ttattgccac atatagggca
     4981 cagtatccac ctagtattta caatatctat atcctcctgt tctaattaac aactcaatat
     5041 tcattttaaa aatttctctt tagttcacta tcaatgtaag ttgattttat taaacatatg
     5101 ccttactttg tctattctat tatttggacg acgtggttgg aaagcagact taatgttagt
     5161 aacctgataa ccttttaact ctgttaaaca gacactgcgt ccatttgtaa agaaagttaa
     5221 actgtttcga tactcttgaa tacaacgggc tggaatttca ccaatgagaa tgacctcgtt
     5281 accttttaac ttagtattta aaatatttgc acaatatttg gaagcatcat tatatgctct
     5341 cgaaagatat tcttgtggta cataaatttc gaagctaaga tatggctcta ataactctgt
     5401 accactcttt ctaaaagcct gctctagtac aataggcgca agcattcgga aatctgctgg
     5461 cgtactgaca gggctgtaat atagaccata cttaaaacag atcttacagt ctgttaattt
     5521 ccaaccgtac aatccttgtt cacacccata gcgtatacct tccataactg cattttgaaa
     5581 tgattgattt aaataaccta gagaaaccag gctctcatac tgaatgccac tacccaaagg
     5641 aagtggtgtt acagataaac caatagaagc ccagaaagga tttggaggta cttcgatatc
     5701 aatggtaaat tcagattttt ttaatggtct ttccatataa atgacagttg gttttcttgt
     5761 ttctatctca atatgatatt tttcttgaat cagagtacaa gttacctcca tttggacctc
     5821 acctaaaaaa gatagcacaa tttcgtgagt tactgtatct acataatatt gtagaagggg
     5881 atcactatcg gatatttcaa aaagtgcatc tagtaacttt tctctttgta ctgatttaca
     5941 tggttcaatt gttgtttgta acataggaag aggattctca agaatttctc tatgtggtaa
     6001 tcttttttta tctccaagta cgttatttag ttttaaaagc tcatttttta aaataataat
     6061 ctcaccaggc tctgccttat ctatctggcg taattctcca tttattgaag tatacatttc
     6121 tgtaactttt attttttcct tttctgatat attgactgag tctcgcaaat gtagcgttcc
     6181 actataaaga cgtacataga caagccgttg accatcatct gaatattcta ctttaaaaac
     6241 atttccacaa agtttatctg aattgagttg tgtgggtgaa aataatttgc tagtaattac
     6301 ctctataagt tgtttaattc caatattatt ctttgcactt ccgtgataaa caggatataa
     6361 ggagcaactt tgaattcttt cgttttcctc cttttcaagt tctgcaatat tcaatgtttt
     6421 cccaatggta tatttttcta ataaataatc atttcccaca attactgtct cccattgttc
     6481 tggttcagta taatctatta cataaggttt caaatttaga ttcacagtct gcttaattat
     6541 gatgtcgtca gaaagtttgt ccttaatatc ttgataaaca tctggcaaat ttattccatt
     6601 ttgatcaatt ttgttaataa aaaatattat aggtatgttc atctttctaa gtgcatggaa
     6661 tagtatacga gtttgtgact gtactccatc ttttgcagag attagcaaaa tagctccatc
     6721 caaaacagat aatgaacggt atacatctgc caaaaaatcc atgtgtccag gagtatctac
     6781 aatatttact ttaacatttt cccgttgaaa agatgttatt gcggtctgaa tagtaatacc
     6841 tctctggcgt tccaaaaaca tagtatccgt tttcgttgta ccgctatcta cacttcctaa
     6901 ctctttaatt gctccgctac tgtatagtaa gctttctgtc aaagtagttt ttcctgcatc
     6961 aacatgtgct aagataccga tattaataat tttcaatttt atttcctcca ttcaaaagct
     7021 caaaaagcag aaaaaatccc agtgataaat actcttatca ctgggatttt aatacaaaaa
     7081 tagctgtata ctacacactg atagccacag atgctttatg ataaaatgat aaaagtattc
     7141 ttaaactggg cacaaaaaac cagtccttat tttaaataag gatgcttaat taattattcc
     7201 cgctatctaa ttgacagttt atttaagaat accttgccgc atttttatta tctccttttg
     7261 aatgttatct taattctatc atatgataga atgcttgtaa agccaaccat attttttgaa
     7321 cgaaagggaa attaatgaga aattaatgga agttagtgta gtagttgaag ataactcttc
     7381 agaatggcta tcaaaattca gcgatttaat aacgatttac aagttcataa gggattcttt
     7441 attcatccag ctattatatt acttttcagg tcaataggac aactgtccac aacctttcta
     7501 caaatatatt acgtataatt tttgaaatat atactgctta acaaaatatt ttgcagtggt
     7561 tccaatagtt ttgaattgtt aatattaagt ttgacttgga aaatatcatt
//
LOCUS       DACDRN010000098         7577 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDRN010000098 DACDRN010000000
VERSION     DACDRN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.098"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..290)
                     /locus_tag="IWJ80_002542"
     CDS             complement(<1..290)
                     /locus_tag="IWJ80_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462220.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINN"
     gene            complement(465..959)
                     /locus_tag="IWJ80_002543"
     CDS             complement(465..959)
                     /locus_tag="IWJ80_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462221.1"
                     /translation="MNKLPEKNRVFRLKGDLSQFKIDENGQFFVWMRHEHCWCNPFEL
                     KKENEIRTKIANGEWDYLFRWEDESFIANDMDHAFVSFLDNTWGTWYDCYLEKLGFQN
                     IDIDAIECLKEVDQKYEEEPYYLLQQKLANNSVFQVEIWENEDGTYEGLAQEIPFSDR
                     KSSI"
     gene            complement(956..1183)
                     /locus_tag="IWJ80_002544"
     CDS             complement(956..1183)
                     /locus_tag="IWJ80_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462222.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            complement(1350..3320)
                     /locus_tag="IWJ80_002545"
     CDS             complement(1350..3320)
                     /locus_tag="IWJ80_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462223.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRLEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAEINVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(3335..4201)
                     /locus_tag="IWJ80_002546"
     CDS             complement(3335..4201)
                     /locus_tag="IWJ80_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462224.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(4191..4424)
                     /locus_tag="IWJ80_002547"
     CDS             complement(4191..4424)
                     /locus_tag="IWJ80_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9462225.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(4436..6592)
                     /locus_tag="IWJ80_002548"
     CDS             complement(4436..6592)
                     /locus_tag="IWJ80_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462226.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAEDVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEDATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIIAVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(6612..6833)
                     /locus_tag="IWJ80_002549"
     CDS             complement(6612..6833)
                     /locus_tag="IWJ80_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462227.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            complement(6943..7281)
                     /locus_tag="IWJ80_002550"
     CDS             complement(6943..7281)
                     /locus_tag="IWJ80_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462228.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNEQLG"
BASE COUNT         2174 a         1483 c         1019 g         2901 t
ORIGIN      
        1 aaattattga ttccttcttc actcatttcc catgtagtat agaaaggagt taactgtcct
       61 ccatactcct gaataataaa atattttcca tcattgatat ggttacaaca gtacactcca
      121 atagttggat actcgactac taaaaattgg tctggatcaa actgttcttt tttcaacatt
      181 tctcgtattt gaaacaaatg atccttctct aagcaatcag gtaattcctt tattctttgc
      241 gttactaatg acccctctat cgctttccat tcatttaaat attgtttcat aacaattcat
      301 tccttttatg ttaactattt ttcctaggaa attgttttaa aacgccactc tcccagaaaa
      361 cgtaagattt ggttagctag aatatacctt ccagctaacc acaattttcc attactatat
      421 tgtaagcttg ctttcacttc ataaaaattg ctgatatact cttttcaaat cgaactcttc
      481 ctatctgaga agggtatttc ttgcgctagc ccctcataag ttccatcttc attttcccaa
      541 atttctacct gaaatactga attattggct aatttttgtt gcaagagata atagggttct
      601 tcttcatact tttgatcgac ttctttaaga cattcaatag catcaatatc aatattttga
      661 aagcccaatt tttctagata acaatcgtac caagtacccc atgtattatc cagaaatgag
      721 acaaatgcat gatccatatc atttgctata aaagattcat cttcccacct gaagaggtaa
      781 tcccactcac cgtttgcaat tttagttctg atttcatttt ctttttttaa ctcaaaagga
      841 ttacaccaac aatgctcatg tcgcatccaa acaaaaaatt gtccattctc atctatttta
      901 aattggctga gatcaccttt caatctaaat acacggttct tttctggtaa cttattcatg
      961 cttcaaccac ctcatactgt tctctcatac ctttgacttc attaaaaaaa actctcgctt
     1021 tttcttcatc agtaaagttt ttatacaact gattacaata atgaggtttt tgaagtttct
     1081 gaatacctat actaacttca aatccttctt catgttgaat taggattact tctgtgtctc
     1141 tacctagaaa tattcgatct aaaattttta tgtttggttt cattatttaa cctccgctat
     1201 aattatttca ctcagtaagg tttcgcttta tgaaatttct atagttctct tttcacaaaa
     1261 cgtaccatgt tctctaagac tgtttttagt ctcaaatata catgtttttt cttatccttt
     1321 ccttatacac tcaaaatcaa agataaaact taaagaataa actctccaat aacaaaatta
     1381 ttagtcagat ccactggtct cctgatctga aatttattac ttcttcctgt tgtcaaatat
     1441 tgtctcaaat caacagaaag tttttgatca tcttctcctt ttctagcagg agcaactttt
     1501 ttttcatact tcattaactc tccactggtc atctttccaa tgatagtgta attaaaatca
     1561 ctttcaaaac tttggtaaat ttttccatct gtattgaata tatcagctgt ttcaatatct
     1621 tctttgtatt cattgtcttc tcctgaaccg attgtatcaa atagaaaggc aagacgaatt
     1681 ccatcttctt caagcattgt taatcttttt ttaattagcc caacggtttc aatagataac
     1741 ttatcgaagc cgtgaatcat aacaatatca tctttttttg cttgtgatgt gatgaaatca
     1801 aaggtattta aaaattgagc ttccataaca ttgatttctg ctcttagatc acctaagtta
     1861 tagtaaaact gtaatttgtc acttttaccg atttctttta gtgtcgttct ttgattaaac
     1921 atcctagaga agctgtctaa tgctgttctc aatgtgtctt ttaatacaat aatacgatcg
     1981 acttcttttt cagtaccaga ttttttggct tttgtttcag cattagtcaa atagctaata
     2041 aaatccccaa gaactggcat tgtatcactg ctgttcacca aaagttttga acgttcttca
     2101 taattaatat cccaaagttt ctgacttata taaaagttac ctaattcttt tttcaataaa
     2161 actctagttg cagaatctaa tgttctgcct gctaacaaat aaaaaatctg agttagttta
     2221 tcgagattcc gtctatataa atcaccaatg tccttttgat taccaaaaag ctccaaagag
     2281 ttaagacctt cttcagccaa attaaccttt tctattacac tgtttaacga aggtttggca
     2341 acaaatgttc taccagttgg atcaccaaag taatcaaatt tatttaagac aatatgaaaa
     2401 gttcgtttat gatttgccat tgcattatta gcaatcaatt gcccccaaag tgatgatgct
     2461 gagatttttt catcatagcc tttaattgta gcgtttttgt gtgatgccac taaaacttta
     2521 cttgtaaaag aatcatccag tgccataaaa gctacaccac ttgcgtagct agtagacata
     2581 gtgccgatag cccatccatt cgcatcatct aaacctttac gaattgcatg atcatttcct
     2641 gcaaaatccg aactcataga cgtatactca tagacattcc cgattggttc acttaaaagt
     2701 ctatcaaaca gatcacgttg atctcctcca tatgacataa gctttaatcc atacattttt
     2761 tcatcataca accgttggag tttaattagc tgttcgctga ttttattagg agaatcggat
     2821 actaatagga tttctagctt ccaatcaata attttatttg ccatgctgtc tagtaaagat
     2881 gctgtattca aatccataat ccgcataaca gttttgctaa tttcagcatc tgaatttttt
     2941 ccttctaaat tattttcata actattcgtt gatcccccaa cattctcgtg cattatttct
     3001 tgttgatctt tttgtgacac tggcttatct gcttcaatta gataggcttt taccccttct
     3061 agtctcactt caggaatagc tttaaccatc caaccaaatc cataatttct attcattcca
     3121 taagtattca ctaatttcac aattgttccc caccgcttac ctgtcctaat gtaagatggt
     3181 gtaaattgat aaccaactaa agatgttaaa tcactaacaa aggtattatt cactaccttt
     3241 gaatttctag ttttttgttc ttttttagtt tttctctttt ttttgaaatt taatagttgt
     3301 gatctaccca taatttccat ttaatctctc acctctattt cagtgcataa atatcagata
     3361 atatttcttt tgtttgttta gctgacaact gagtgacact ataataaagt cctctactcg
     3421 tatagttttc taaacgttcc acaccttcat ccaataccat cttagattga gatttcaaga
     3481 taagatattg gattactgtt gttttcacac catcaatata ttctgataca aatcgttctt
     3541 gctgatctat tatgtcaaag actgctttgt tactccttgc atttcgtttt tgtattttca
     3601 tatttcgagt ttgtcgttcc gtattctgag gttgagatga agtgattttg atttcagcta
     3661 aggaacgatc tctggtattt tgatattgta ttgatttcga ttcctgagcc ataacttctt
     3721 ctggaaaagc ggttagtgat gtagccccat ctagattgaa cattaatgct acactcccat
     3781 ctcgatatac gattgttcca tcttgtttca cttttaaaat ctctgtgcct cgaaatggtt
     3841 tctttttacc catagatact cttgctgaat taactactga tttgaacagt ctaatagaac
     3901 cagaaatcaa tgaaccacta gatggatcaa taaacatata ctggctaatt agaataagaa
     3961 atccaattat cattaataac gttggaacaa aaataaattt cagtttaatc gtatcagaaa
     4021 ctaagtaaac aattgatcca agaacaacga ctgcaacaac tatagctgat acaattaatc
     4081 tttttgctgt aacagaatag gtgctattga catgcttttc aagccattca actctgacaa
     4141 ttgtttcaga aggtttttga tgtaagttat gtttaaatcc atagtttgag ttatctgcca
     4201 tcagaaatac ttcctttcac gttcaactta cggtaaacac ctacagttat cactattgca
     4261 acaataagca agccaattaa agatattgac ttgctcattg tctcgttcgt ttgtggtagt
     4321 tcatttacct gatacacgag ctgtgcttcc tgtaagtgct gagaagaggt catgttttct
     4381 tgattgtttt gaattactgg ataaactatt ttttcttctc gcataatagc cctccttagt
     4441 tattatcaga taagtgagct tgaattttgt actgatttcc gtctggagtt ttcacaactg
     4501 ctttttctcc atcaatagaa atcacttcag ccttagttgt tttcccatca atactgatga
     4561 caacatcttt cccaactttg aactctgtag ttggtgttgc ttcatagcta ctatttgttg
     4621 aagcagagtt cgtatttgtc ccaatcgaat tttccactct cgaaatagat gtttgagttt
     4681 gagcctgtgc ttgttgatta ttatgactaa taaagtagga cgatcccaag atcgttccgc
     4741 aagccattgc agcgacagca atgatagtag ccatcttttt acttgtaaaa tcacgttttg
     4801 aacttgattg attttctgta gcaactgcta atgatttcgt tttttctgcc atttgagcta
     4861 gatcacttcg ggaacgagat aagtcttctc ttaaagcttc actttgttct gccaactttt
     4921 cgctatttaa tttataaccc tctgctaaat ctttgacctc tttcattcga tctttatatt
     4981 cttcgatagt tttttgttgc tctacaagtt ttgtttctaa ttctttcgtg ttgttagcta
     5041 cctgcttttc aacttcttct ttgttcgcaa aatatttgac aaccacatct gttgttttct
     5101 cttttgtatc atggaactca gcttcatttt ctgactttgt tttttcgaca atctctaaag
     5161 ttttttcttg cacgtctgtt aatgattttt caatttctgg tttcaatgaa ttgataattc
     5221 gttccaccac aacttctttt gcttcttggc tttgtttcaa tgcatcgttt ttaatagatt
     5281 caattgcttc ttggtactgt tgatcaagac tttcttcagt tgcttttcta tattcatctt
     5341 cttgttgttt aacttctggt aaatgttcag catcataccg ttgctctagt ttctttagtt
     5401 cagcatccaa ataagcctgt ttcgccatat cataatcttt acgaatgata ttggattttt
     5461 ccatcactaa atcatcaatt ttagatgctt ctgcttcgta gctctcttta gcaaccgata
     5521 gtgtatttgt aaactgattt tgaccattta atgaggttgc atcctctatc cgcaaaagct
     5581 catcggctaa catttgcttt cctttgagga tcaattgctc cttcgtctta tttaattcac
     5641 tgttgatttt attattagca atagagatac gagcgttcat actctcttta gctaaaaaca
     5701 gcaatggttt caactcaggt atggatagca tctcctcgtt taaatcgaca gggtcattaa
     5761 tctcatctaa aactacaggt tgaaatttgt cagcaatgat ctgttcaaaa tttttttcta
     5821 aaaaatcagc atctaagttt ttcggtacat cactttcaga tgtgattttt tcagcttcat
     5881 aaacataatt ttctttgtca ctttgggatt cgggtttttc atcaattttt ggttgttggt
     5941 aggtttctgt ttgtatttgt gtttcttcta tgcttacttc ttggttttct gcttcggttg
     6001 gtttgttatc atccacaaag agagcttctt tttcatctgg tacatcttct gctacggctt
     6061 caaattcaac ttcttcagaa gagttgaagg tctcagcaat tcgttctaat ccattagtag
     6121 ctacttctaa ttgctctact gcgctataga agtctgtagc ttcttcaact tctgattcaa
     6181 acacattagg atcatccgga ataactaata attgaccact actatccata aaacggacac
     6241 ctgctaatag cttctcttta aatttttctg gtagatcagc tacataattc aaacttgact
     6301 tcaatagaac gacatacttt tcagaatcga gaatagcatc atcctcatct ataactccct
     6361 cactgttgag cttttgtaca tcttcaatta ccatataatt tttctttggt aaatctttgg
     6421 tcagcaacgc atcgaattct ttccatacct ttgctttagc tttttttgcc ttcgcttctt
     6481 tttcttcttt tttgtacact tcgagtaatt gtggcttcaa agagagtgca actacaggaa
     6541 ctgtttctgc cacataatta atagattcgg taatatcttt gaattttgcc attattgtaa
     6601 aactcctttt ttcagtcaat agttcttttc tgtttgtcta gcatatatac atatgctttt
     6661 tcgaatttaa taagcgtttc aagtctggct ttttcaatct ttttactccc atttcgataa
     6721 tcatagaact ggttaacatt catattcatt atctttgcca gttcggtacc tttcagactg
     6781 ctcattaata cttcgttggc gtgagctaac attttttctc tatcaaaaac catcttacat
     6841 ttactttctt ccctcacttc taaactacgc ttggagcgta gttttttggt aaaaagtaaa
     6901 ttgaacaaat cggcatatta tccaaatttg ttcaattcac ttttaaccta actgttcatt
     6961 ttttggaaat tttgaaacta gatagatacc aacaactgga ataagtgctg atatgatatt
     7021 agctttttgg ttcaaatcat tacctgtttt agtacccatt gtgtaaagca taacggttgt
     7081 aattacagga ataataaaga ttgcaataat cgccccaaca tattttaatc catctcccca
     7141 ttttgcatca ccaaaaaatt tcattgcagt gaatcctggt ttcaacgaaa aaacgaatgc
     7201 caagaaatac aaaacaactt gtactaaagt tttcatatca ggcgtcattc cgctaaccaa
     7261 atttttaata gaatcgaaca tataaaaacc tcctttttaa tatttttaat aagttaatta
     7321 tatcatcgac aaatcttatt tgttcacttg tcaatgatct aactaagaaa aagacgtctc
     7381 gatatttaca tctttatgtt atcataataa cataaatttt cgatgattaa aagagatttt
     7441 ccattaaatt atttcacttt ttttattggc taaaaatgct ggtttatcaa gtttttgttt
     7501 agataataat tctgttcttt accagcgaga gtataaaata ttttgtgtaa atgaaaaaat
     7561 ccatacaaaa aaggaag
//
LOCUS       DACDRN010000099         7213 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDRN010000099 DACDRN010000000
VERSION     DACDRN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.099"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(35..2146)
                     /locus_tag="IWJ80_002551"
     CDS             complement(35..2146)
                     /locus_tag="IWJ80_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9462229.1"
                     /translation="MEGIMENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEDRLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(2435..3259)
                     /locus_tag="IWJ80_002552"
     CDS             complement(2435..3259)
                     /locus_tag="IWJ80_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462230.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            complement(3256..4011)
                     /locus_tag="IWJ80_002553"
     CDS             complement(3256..4011)
                     /locus_tag="IWJ80_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9462231.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            complement(4024..6000)
                     /locus_tag="IWJ80_002554"
     CDS             complement(4024..6000)
                     /locus_tag="IWJ80_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9462232.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            complement(6053..6724)
                     /locus_tag="IWJ80_002555"
     CDS             complement(6053..6724)
                     /locus_tag="IWJ80_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9462233.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(6784..6993)
                     /locus_tag="IWJ80_002556"
     CDS             complement(6784..6993)
                     /locus_tag="IWJ80_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462234.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            complement(7097..>7213)
                     /locus_tag="IWJ80_002557"
     CDS             complement(7097..>7213)
                     /locus_tag="IWJ80_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9462235.1"
                     /translation="TIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT         2000 a         1466 c         1095 g         2652 t
ORIGIN      
        1 aaaacaaggc tgattctcag ccttgttttt atatttattt gagttttaaa actacatagc
       61 ccaatgatgc taaagtaaga aataatatac ttccaataac agttagcact aaatgattcg
      121 atccagtttt tggcacaatg atctgaggta agttaggttt taccccaatc gttactacat
      181 tagttgcttt ctcaatactt tctactgaaa cttttgcttg gttgtcgata tccaattctt
      241 cattcaaagg agttttagct gtaaatatca gctgaatttc tcgtccatct aaaagatttt
      301 tacttaagaa atcagcggaa gcttcccact tcagcgttct tgaattgcta tcataggtta
      361 gtgtaccttc tgaagtaata tcttttcctg tactcatatc tatcactcgc gcagaacctt
      421 ctttatacgt caaacgatct tccactggat cttgaatact aaattgcttg taatgatctg
      481 aggcaatgtc tacacctttt gtatgccatt tttgtgtgac tagatattgt acttcagatc
      541 ctgcatttaa aggagctgta ggcgttcctt ttttaccatc atttcgacca tcaacatctt
      601 tcccatcttg ttccactttt ttatttggtt taagagagaa atagttttct actgtatttg
      661 ttttttgtgt tcctttgttc ggatcattgt taaacgttac agtagccaca tttggtgcaa
      721 ttccttttgc atcatttttt gctctcatat ggagctgcaa aatataatcc ctaccgtaat
      781 ttgcagcgtt agctaaaaag ctgtcagaag cagtataagt gactgtatta cctgatacag
      841 ttaactttcc tcctgtatca gcttttttcc aagtaccatt cgcttcaaca taccacaatg
      901 cgccatcata gtttttccca tccgatacca tatcaaaatt tttgctgtca aaggtatcag
      961 tgattttaaa tgatttgtat ttgttcattg tgttgactgt taatgcatca aacttttggt
     1021 gaatatgata gtaaacatct gatccttgtg gaatcacttt tttgtcaata ttgtgtgctg
     1081 cctcttcagt attagctttg ttaaacactt cttttgtagg cttcgatggt tgtggtgtcc
     1141 atagcaatgc agtatcaaag ctaaacatca tactattcgt atttgcgcca atcatgaatg
     1201 ttggggaagc gccttgaagc gtatacgtta caccacttct gtgataagta tcagctccta
     1261 caacatcggt gaaatgatca ttcgagctat aagccttttc tgcacgtcct cccactactt
     1321 ttgaagttaa attgtttgtt gataacgcag gagctttgta ttctgcacat gctgtatctg
     1381 ttgtgacaaa ataaggtaag ctacccattt tttcataccc aacaaattct ctcatatcaa
     1441 tattacgttc accgacagat tcattattgt cattatacca agacacacgt tgctgatact
     1501 gtccatttaa actgtaaatg gatagaaatg tatcgtttaa atcaatacca gctgctctca
     1561 tcgatctcat ttttgattga atttctggct gtggttcact caaagctact tttgtttttg
     1621 ttcctgcata cttaaattct agttttgcat tcatgacatt gattgactgc atgaaaaatc
     1681 ctcgataaat atttttatca aagagcaaat aattataata ctttttgcca ttaattgttt
     1741 tagctaatcc gtcttggtta accccagcgt gcgctacaaa attagaaaaa gtcacgtcac
     1801 aatcaatttc ttttcctaga taagtaccta ctttttgata atgggcaacg atttttgacg
     1861 tacttttcaa atctttgact tttggccaag caagtacttt ttgtttttta gttgaatact
     1921 catctagtat cttaaatcca ttgatcccag ttacttcgat atttctcaat gaatcactat
     1981 catgataaag caccagatta ttattttctt tgatttgact cggatcaact ttttctaagg
     2041 cctggactgc agttcccctc gcaaaaagaa gaaggaacaa aaacaacact aaagtaatta
     2101 ctcttcccgt agtgcaatga attttatttt ccataatgcc ctccatctaa tattttttta
     2161 aacgatcgta agcttttcat tctttttttc ctaataccca cgttaccaat cctgtaagaa
     2221 gaaccaaact aaccataaaa attggattga tcaagagctt tttaatcacc tctaatggca
     2281 tggaagcaca ttgaatgttt agaaaatgaa tgaacaatcg ttgatatctt ggataaattg
     2341 ataattgaga aaaaattaaa ccgcaacaca atattgttgc ggctaaaaag gatagattaa
     2401 aaactaatat tcttattatt ttcatcattt catcctaatt atcaaaaaat aattgagcaa
     2461 aaaattccca acaattttca aaaaagttct tctttacacc agttgacaca acttttttaa
     2521 tttcttcttt cggctctgat ttctctgatt tagggtataa ccaaacatcc gtcaaaaatt
     2581 cacctttttc atttgaaaca ggtgtcaata aaacaatatc ttcactgttt gaaatgtttt
     2641 cagggcttga ttcattaacg atatagtatg cttgtttttt gtttgcaggt acttcaatgg
     2701 aaaattcaat cacgccttcc tgatcatcaa tcgttttggt ttctccttca gccacttttt
     2761 tcaactggtc gtatgaaagt ttttttgctc gatctttcaa ttttgactca attatttcta
     2821 cagattgatt tttctcatta tcatcttgtg acatttcttc taagatattc gtgatatcgt
     2881 agacaataaa cttcgcgcca ttgatacctt cttgctgggc tacttgactg ccttgattgt
     2941 tgatttgatt cgaagaaata attttttgaa tatgaatggc ttgcttgatc acttgcgtat
     3001 cttctacgtc ttgcggcttt tctgaagtct ctgaagaact ttctgacgtt ggattcgttg
     3061 attcagtaaa gcttatattt gaatctgccg tttcttctgt agtattctcc gaactgtccg
     3121 tttcgcttgg tgcagtaatt gaattatcta ttgtttgagt ggtactacta tcactgctgt
     3181 cagcgaaaga cagttgcgaa taagctacag tagaaaagag agctgctaca caaacaaaca
     3241 gccattttct cttattcatt ccgatctcct ccttattttt ttataaataa aaataatagg
     3301 aacgcaaaaa aaagcgaata gcagtgctaa tcgccaatcc aatgaaattg atttcctatt
     3361 ttttgctttt ttctgtagtt ttaaatcttc tttactgaag gaaactcgtt caccatacac
     3421 taaaaagcgg tgtgagttga caccttttgg tgtgcatgtc attaacgtga tcctatcttg
     3481 gtctttttca ggcataagat aatctaaata atttttttgt aattcttctg ctttaacttc
     3541 ttcaattcga taaatttgat aggcatgttg ttcttcattt accttaataa agaatttttg
     3601 cccttttttt actgaaggca aatcatcgaa aagattatct ttataaagtc cactgtgccc
     3661 cgcgattaaa ggatttttac cgtttcctcc tgtaatatcg ccagttcctt ccgtaatccc
     3721 tacaccattc tctaaaattt tgtcggaagt tccgtcataa attggtagtt ttaaagaaat
     3781 acttggaatt gtaagaacac caacaggatc gcctaactct attttcgcaa catccaaaga
     3841 agaatctgtt ttttcaagat tttcaatggc taaaattctt tgctgttgtt gaaatttcgc
     3901 tacatttgtt tccacaactg gaattaaata gactgctact gccgacagca accaaaaaaa
     3961 tatcatcagt ttgtgcatga ctcttctttt tgggggctta cgaataatca ttagctgatc
     4021 cttctaggct ttcttcttaa agtaaacaac tgcagaaagt ccccctgctc ctagcaacaa
     4081 gcaagagatc aatacgtaag attggaaacc gttgctacca gtcattggta cggaagcttt
     4141 ttgattattt ttgatcgtga ttcggttttc ttctttgtat gaatctttgt ctactttgaa
     4201 agagatctct tttgttaatt tttgatagtt ttcaggagct tttgtttctt ccagtgagta
     4261 atctccttca gttaaaccag tgatttcaaa atgaccctct ttatctgatt ttaggattgc
     4321 tgcatttgct acatcgtcta cccaaactgc ttctttgaca ccatctttat ctgtatcagt
     4381 aagtccaccg tagtacttac cttctgcatt cttagctgca aattctgcgc cttcaagcgg
     4441 tgttgctttg atacctgttc caaataggcc gctatcgact tttaagaatt ttgcgccata
     4501 agtcacaacg tcagctttcg ttttttcgtg gtgttcttcc tgattaaatg gattattgtt
     4561 ataaacgaaa tccacgctat ttgttaatgg atttgcgatt tcagcactat cattcacaaa
     4621 catgttataa gtcattttta gatcttgacc atgtaaattc gcaactgtat cacttacttg
     4681 tccattattt acgataaaat cgatcacaaa accactatca gaattttcga ttttatagtc
     4741 agtattttct tttaatacga ctgaaccatc attatttgtt actttaatat ctgctttttc
     4801 agcaacataa gaaagtcctt tacctgctgt atctccccat ttaaatacac tgtaaggaga
     4861 agtttcgctt ttcacaccgt ttactgtgat accgtctgcg ccacctgcaa tgtctgttgg
     4921 aatatgaatc gttccttgat aatttactgc gtcaccaatt tggaaagaat gttcttgtcc
     4981 tgttccattt gttactgttt tatcgatcac tggtgcttta tagtttacgt atgaagcttt
     5041 taagtcactt gtcacgccat cattcgtaat ttcaaaggtt aatttattgt gcgcatcgtt
     5101 gcgagcgtct gcgcctaata aatattgatc tgctgttggt tcttgatctg atcccactac
     5161 aacttccgaa ggaacttcca caaaatagta ttttcctaac gtcaaatcag tcgcagtcag
     5221 ctttccttga tcatccgata caagatcacc caattttgtt ccttttccag gttcgccctt
     5281 gtataactca aattttgccc cttttaaaag ggtttgtttg ctttggtctg cgccatcgtc
     5341 accaaatttc gttaattcaa aactcaactt agaaacaatg ttcttaggat acaaatgaat
     5401 attttttaaa aatcctgatc ctgttttatc tgtcattggc gcaatcacaa ccattggttt
     5461 tgctttttgt gtgactaatc cagcagcgga tttactttca taaaccacat atacattccc
     5521 tttggaattg acataggctg gttgatcagt aaaagtgatc tcaccatttt catctacagc
     5581 tgaagtaatt ttgttggttg cattcttgac gtactcactg ttagatccct tgtcttctac
     5641 atcttgtaca atctcgtcca ctttcgcgtt ggttagatca gaatcttctc tcggtaatac
     5701 ttgatccgta atatttccta acgtaaactc aacatcacca taatctgcct tgttgtattt
     5761 cgttacacct gtaggatatt gatcgtgcgt ataaccatcg tttttgatcc cgtcaatatc
     5821 aacattcaat tgcgttcctt gatcgtacat taatttatga accgtgatat tgaccttctc
     5881 tggtggtgtt ttttgcgtga catcttctgc atgtacttga tacccctgaa agcagcctgc
     5941 aatgattcct aacattccaa caagcattcc agcttttttt acataccgat ttattgccat
     6001 tttttactct cctcatttca tatttcaacg actcgcgttg attaaaacct tgttattgta
     6061 gctctccctg taccacgtat ctttcttttc cttgtgacgt ataaacacaa gtaaacaacg
     6121 ttaataaggg tttctctcct ttctttggca aagacaagaa agagttctct tcaggaacgc
     6181 catcgattaa tttaaagtac ccaggtacga taaatttacg cgttaccttg taacgataga
     6241 cgccatcgtt actaactaag tccatcacct cccctaatga caacttttga atatcagaaa
     6301 ataaaacacc tggcatttcc atgttatgac ctgctaggac atagtttcct ttgcccattt
     6361 cggcatcttc ataaaaataa gtagccgctc caagactcaa agtgtattga tttgcccctt
     6421 taaaaattgg caatgctaaa ccaagtgcag gaatctgaat ttgtgcaaat acttgtttat
     6481 tgacgcccat ttcgattgct tctttttgta tggacaacat ttgccccaaa gacatatcat
     6541 tattgacgtt cgttgcatcc tccttataac tctttgcatg tgcattgatt tgatctacgg
     6601 gcatcttttt ttctatattt tgcttaaaca cctctccttc atgtttttgg tagctaacat
     6661 agccaaacac actgcaaaag acgattaaag tgactcctaa aaaaccaaat aaccattttt
     6721 tcatccatct gcctcctttc agacaaaaca aaaaaacttt ttctcatttc catagaaaaa
     6781 gttttaaaaa tgaattctat aagttcgtct caactgttca aagtttttca ttgaacctaa
     6841 caaacaagct ttctcttttc ctgttaactg catatcattg atatattgcg cgacagataa
     6901 tcttttaagg tgtttttgct tcatcgtttt tagtaacttt tcttccattt ctttcgacac
     6961 tttttttctg ttttcgattt cttcataatt catgagtttt cctaccttcc ttgttttttg
     7021 caaaaataaa aaagcctatg atggttctgt tgcaaagttt taaataaaga ataaaatccc
     7081 ttacggtatc tatgatttaa gctgggattc ccaataatac cttgatttca gtacagaccg
     7141 aaaacccgaa gagagtgcct tcttttcggg ttttcttata taatcctcgg atggcttcca
     7201 tgcctttaat cgt
//
LOCUS       DACDRN010000100         7175 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDRN010000100 DACDRN010000000
VERSION     DACDRN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.100"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..453)
                     /locus_tag="IWJ80_002558"
     CDS             complement(<1..453)
                     /locus_tag="IWJ80_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9462236.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLS"
     gene            complement(573..1220)
                     /gene="eda"
                     /locus_tag="IWJ80_002559"
     CDS             complement(573..1220)
                     /gene="eda"
                     /locus_tag="IWJ80_002559"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9462237.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1304..2731)
                     /gene="uxaC"
                     /locus_tag="IWJ80_002560"
     CDS             complement(1304..2731)
                     /gene="uxaC"
                     /locus_tag="IWJ80_002560"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9462238.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(2805..3884)
                     /gene="uxuA"
                     /locus_tag="IWJ80_002561"
     CDS             complement(2805..3884)
                     /gene="uxuA"
                     /locus_tag="IWJ80_002561"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9462239.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(3887..5518)
                     /locus_tag="IWJ80_002562"
     CDS             complement(3887..5518)
                     /locus_tag="IWJ80_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9462240.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            5822..6493
                     /locus_tag="IWJ80_002563"
     CDS             5822..6493
                     /locus_tag="IWJ80_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9462241.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT         2309 a         1413 c          972 g         2481 t
ORIGIN      
        1 cgatagcatt gttctttctt ctgtaataga agtaatagct gatgccattg atccataagg
       61 aatatttata gatgtgtaaa aaatactccc ccataatata tacgtaacat acatgtatac
      121 taccttaacg ctcatagaag ctgaaatcat agaagtttga tacattaaaa aacttgctaa
      181 agcgactgga ccactcattc ttaaaatcca cggtttaaac ttaccctgct tagttgcttt
      241 cgatctatca acaatacgcc ccatagttac atcagtaact gcatcgataa atcttgctac
      301 taaaaataat gttccaacgg cagctccgct tatccccaat acttttgtat aaaagatcat
      361 taaaaaagaa ctagcaaaaa tgaaagtaaa atcgtttcca aaatctccaa acatatatcc
      421 tattttgtct cttataccaa attttctttc catacaattc tctcccttct gttgttaagc
      481 gttttcattc tgatgaaagt acgtactata ctctgaagcg taaagagtca gtatttattt
      541 tttacatttc agagtattat ttgttttttc tcctaataat tctttatctc ataaaactta
      601 tctatatatt tactagctac tcgacttact tcttcaaaat cacctttttc aacagaagcc
      661 aataaattcc cacctacacc aactgcaaca actcctactt caaaccaatc ttctaagttc
      721 tctatactta ctccgccagt aggcattata tttacgtgtg gtaatggagc ttgataggct
      781 tttacaatgg atggaccaaa agcagaacct gggaatagtt taatgatatc tacgccagca
      841 gtaagtgcct cctttatctc agtaattgtc atacaacctg gtaaataagg aatttgatat
      901 aaattacaaa tctcagctgt ttgtcgatca aaagatggac taacgatata ttctgcccca
      961 gccataattg cgatacgagc agtaaacgca tctaaaaccg taccggctcc gattattaca
     1021 tctttttgat cagcatattt tttagcaatt tctttgatta tttcttcagc attaggaact
     1081 gtaaaagtta gttcaatccc tttaattcca cccgcaataa ttgaatttat tgctttaatg
     1141 gcctcatcct tatttttccc acgtacaacc gcaatgactc cagccgcttc taacttactt
     1201 aaaatttcta cttttttcat atctatctcc ttcctttatg gatacatact ttgtctagct
     1261 gtactaactt acaaagaata aatttaagct tttatttcaa gtattatttt ctacttccga
     1321 tttcaaaaaa attaaaataa ttatacgcat tacgataaca aatattctcc actatctgac
     1381 taagatacat ttcatcatct ggaattcggc ctgctttaac ccactcgctt agtaattgac
     1441 ataatactcg acgaaaatac tcatggcgag gatagcttag aaaacttcga gaatcagtca
     1501 acatacctgt aaaattcgac aacaaacttt gttgtgccat cgtagtcaac tgttgacgca
     1561 ttccttcagc tgtatcattg aaccaccaag cacatcccaa ttggattttt tgaatcatac
     1621 ccccttggaa gctttgcatt gctgttgcta gtggaagcca atcagttgga tttaatgaat
     1681 ataaaattgt ttttggtaag cacccactct tttcagcggt atcgaacaat gctacaacat
     1741 tttgtgtcaa actagcttga tcccccatcg aatcaccacc gacatctact ccaatttgtt
     1801 cctttaatat acttgaatta tttcttaaag catttaaatg catttgcatc gtccaaccat
     1861 atcttgtata ctcagacatc aaatgtaatt gaatacctgt aacaaattgt gcaatttctg
     1921 ataaatggaa atccgttttt cctgctaaag cttttattaa cagattgtcg agtatttttt
     1981 catctgctat ttcataaaaa taagtattca aaccgtgatc tgctaatcga cagccaattt
     2041 gatgaaaatg atccactcgt tggctcaatg cttgcttcaa cccattaaag tctttgattg
     2101 gtatagccgc tgtttgacta agttttaaaa tatagttcga aaagcctgat gatttgatac
     2161 tccagacctt atctggacgg aaagtaggta ataccttgaa agaattatcc tgtttagaaa
     2221 gctcagtatg ataccacaag tcatctgtag gatcatccgt tgtacagatg gctttcacat
     2281 ccatcatctc caacaattct ttcgggcgat aggaatccgt ttgaattttt tcgttagcac
     2341 gtcgccaaat ttctggtgca ttcttctcag aaattgtcaa atcaatatca aagtaacgtt
     2401 gtaattccaa atgagaccat tcaaaaatag gattacctgg cgccttctcc actgttttta
     2461 caaaagctaa aaatttttca taatcatcac ctggaccact gatcaactct tcttctatac
     2521 cattagctcg catcaatcgc catttataat gatctccagc tcctttatca tttaaccata
     2581 cctgagtcaa attcttgtat tgcttatttt ctagcaaata tgctgggtct aaatgacaat
     2641 gatagtcgat aattggttgg tgttttgcaa atttatgaaa aagtctttta gctatttcat
     2701 tctctaataa aaagttctta tctaaaaaca tatttaaaca ctcctttcct tccgttagtt
     2761 tttagaagag aaaatttttc ctctaaataa actcccttat ataattattt actcgccccc
     2821 ttttcaagcg cttcccaaat accatttaaa taaactgcac ctagagctcg gtcatataat
     2881 ccataaccag gtctaccggt ttctccccaa atcatccgac catgatctgg acgaatatag
     2941 ccattaaatc cattatcgta cattgttttt aatatcttat acatatctag agaaccatat
     3001 tctgatttat gcggcgcttc gtaaaaatcg agtccattgt cacttaaata tttgatatta
     3061 cgaacatgtg caaaaggaat ccgatcccgt tgacaaaatt cagatagaat ttcatagaca
     3121 tcattatctg gtcgttctgc aatacttcca gtacataatg taataccatt agaaggagaa
     3181 tccactacat tacacaacca atctaaatcg tcacgatttt taacaatacg aggtaatccg
     3241 aaaatggaat atggtggatc atctgggtga actgccattt taatcccaac ttcttcacat
     3301 gtcgggataa ttgctttgag aaaatatgca aagttctctc gtaatttctc ttcatcaact
     3361 tctttatagg cttctaataa ttgcttaatt tgtgataacc gatgaggttc ccaacccggc
     3421 aattcaaaat tattagtaga attttctact tctctaataa tttcctccgg atcagtaggg
     3481 atattttttg aaatataggc taacgtagaa gacccatcat ctaaacgata atccaaatcc
     3541 gattttaccc aatcaaatac tggcataaaa ttataacaga taacctcaat cccaaattct
     3601 gccaaattgc gaatcgtctg tttataattc tcaatgtatt gatctcgtgt tggtaaacca
     3661 attttgatat catcatgaac attaacactc tcaataactt tcaaggtaag tccattggct
     3721 tcgacttcat cttttaaaga tttaattcgt tctctcggcc aaacctctcc ggctggtata
     3781 tccattaatg ttccaacaat tcctttcatt gctgggatct gacgaatttc ttgtagtgag
     3841 attttttctt catttgcccc ataccatcta aaagtcattt ccatagttac tttcctccaa
     3901 cttctgttat atatttattg attgttttac gaactgcttg aggacctact agcatttcgc
     3961 aaaagatttt ttcagttttc tttcctaccc caacttgata taaatctaaa ccaaagattt
     4021 ctttattttc taaaatttcc tgtaccgcac aatgaatctt tgaaggatct tgttcaccta
     4081 atttcaatac tgataattta tcctgcaact ctaccaataa cggatcagga cttggcacaa
     4141 aattttcccc ttgatcatcg actgccagta aataacgtaa ccaagcagca atagcgatag
     4201 gaataaatgt tagattttct gcatttccgt ctttttcgac atacttttta attgtttcac
     4261 catagcgaat cgctactttt tgagaagtat ctgttgcaat tcgttgtggt gtatctggaa
     4321 tatagggatt aggcaaacgt ttattaatta cttcatcaat aaattgtttt ggatcaataa
     4381 tgcctggatt ttcaactact ggtaaacctt ctaaataacc tattcctttg attaaaccaa
     4441 ccaaatcatc atcctgcatc tctgctgaaa ttgattgata tcctagcaga caacctgtta
     4501 ctgccaaagc agtatgaagt ggattcaagc ttgtcgtaac cttcatgatg tctgctttat
     4561 caaccgtctc tctatctgtc ataatcacac cagccttttc caatttaggc cggccatttg
     4621 gaaaactatc ttcaataact aaataatgaa ctacctcagt attagtgaaa gcagcaatat
     4681 tggtatgttt ttttgtgtga atgatatcta aatcctcgag ccccatcatc aatagatctt
     4741 cctttacatt ctctgaagga tttggcgtaa ttcgatcaat catactccaa ggaaaagtca
     4801 ctttttcttt atcctttaaa taagcaataa atttttcaga aacatatcct tctttttgcc
     4861 aagcttctgc aatcgtaaaa atactatctt gaaaacgttg accattctgt gagaaattat
     4921 cagtagaaac catcgcaatc ggcaactctc cagcattaaa tctgctatat aacaaggatg
     4981 ttacaatact catcgtatgt tgaggattgc taggtccaga tttgatatca gctttgacta
     5041 ccgaaaagaa ttgcccttta gaatctttta aactgtaacc tttttcagta atagtaaacg
     5101 ttgtaaactg taatttagga tcttcaaaat atttaaccac tctttgatag tccgctatat
     5161 tcttcggatt gcaatataaa ctatcagccg ttactgccaa aagtcttttt ttgagttcac
     5221 catctccact cataacaatt tccaaaatgt cattgttata gtcatgataa gctttttcca
     5281 ctacttcttc atcaaatgtt tcacacacaa ctactcctgt ttctaattca ttttgatctg
     5341 ctaagcattg agcaatttct gcatgaaatc ctcgataaag atttcctcca ccaaaatgaa
     5401 gccatactgg atgttgctgg ccttcttgct taacttcttt gacatcaaaa cacggcagta
     5461 gaatctgcat ttcattgaat tcttcgatag aattttgcca atttttaatc gaaaccataa
     5521 ttaacctcct caaactgata tgttagtttt gatacatttt atattttaaa acccattgta
     5581 accgttgtca atataaaaag ggaataaact tgcttttttc taccatatca gaatgattgt
     5641 attttttatt cagaaaaata aaatactcct tctttttgga ctgattttat cgaaacttat
     5701 tttttctttt tttcatgtat tcttttattt ttataactag tgtattactt caaaatttga
     5761 tattatatgg catgaacaga attaaaatat cagttaaaat aaaagttaag gagcattgtg
     5821 tatgacaatt aaaactagtc tccaaaatca agcttaccaa tctattcgcc gacaaattat
     5881 ctatgctgac ttagaacctg gaaaaaaagt atcagaaaaa aaattagaag aaacactttc
     5941 tattggtcgt acaccaatac gtgaagcact gattcaactc cgacaacaag gtcttgttga
     6001 tacaattcct caaagcggaa attatatctc attaattgat ttagattcgg ccaaaaatgc
     6061 tcgatttgtc agagaacatt tggaacgaca aattatgtta gagtgttgtg ccaaaatgaa
     6121 taaccaaaaa aaacggattt tagaaactat aatttcagaa caagaaaaag ctgttagtca
     6181 aaaaaatgct cgtagttttt ttcataatga taatttattc cataaagctt gttttgaaat
     6241 tgcagagcga gaagaagtat ggcaatggtt agaaaataac aatacacact tagaacgatt
     6301 tcgatggcta cgagtaaaaa cggaaggatt aaagtgggaa aaaattatgg aacaacatta
     6361 tcaactattt gaggcattaa caaaacaaga tccagaagaa gctaactttc taacatcgtt
     6421 gcatcttcat ttaatgctta atgagcaaaa tattgtttta gctactcatc caaaatattt
     6481 caaaaatata taacttttaa ctctttttaa attaaatggt tgttctgatc agaaaaatat
     6541 cgcttgtttt cttattatgt aatatatttc ataaactatt ctgcataaca actattgttt
     6601 gtacttttta cattggataa ctaattatga ctgctgataa cttttgatat taaactagta
     6661 tctccaatac aaataaaaaa tagatatctt gttcatcaaa attagttcgt ggatacttac
     6721 cttcatttac atcattcagc ggtttcccat tttcttttat tctattattt cattttctaa
     6781 tagacttggc ttcatttcgc aatacttttt ttcaataggc atcaaattgg tgttgtttag
     6841 atatttagtt gtagttttgt gacatgataa gtatgctcct ttttctcttt tccactaata
     6901 caacgatcaa agggtgcttt tttcaaaaaa ctagaaaaat caaaataaga ttcgtatttt
     6961 gattctatat atcctgtttc aactacatct tatatttcac aatagaataa atagcctttt
     7021 gcatagctgc ttaacttata agaaattgca atttacttct tcaaagtgaa aacgaatatg
     7081 agtaaaataa aattattctt cttacagata attaaaaata gcgcgtaaca aaaatcggca
     7141 ttcaatcaca taattaggat tgatgccgat tactt
//
LOCUS       DACDRN010000101         6967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDRN010000101 DACDRN010000000
VERSION     DACDRN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.101"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(644..1624)
                     /locus_tag="IWJ80_002564"
     CDS             complement(644..1624)
                     /locus_tag="IWJ80_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP9462242.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(1637..2515)
                     /locus_tag="IWJ80_002565"
     CDS             complement(1637..2515)
                     /locus_tag="IWJ80_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP9462243.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(2531..3331)
                     /locus_tag="IWJ80_002566"
     CDS             complement(2531..3331)
                     /locus_tag="IWJ80_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HAP9462244.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            4723..5049
                     /locus_tag="IWJ80_002567"
     CDS             4723..5049
                     /locus_tag="IWJ80_002567"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9462245.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            5057..5965
                     /locus_tag="IWJ80_002568"
     CDS             5057..5965
                     /locus_tag="IWJ80_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9462246.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            6594..6845
                     /locus_tag="IWJ80_002569"
     CDS             6594..6845
                     /locus_tag="IWJ80_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAP9462247.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
BASE COUNT         2093 a         1410 c         1166 g         2298 t
ORIGIN      
        1 taaaaaatat gcggcgcaca gaacgcgccg catatcggac taagttttat tcaaatttgt
       61 cttcgccaac acaatttgac gaagagcctt ttctttcagg aagacttcgg taatttttag
      121 gagttctacc tattctttta accgtttatt ctctttttct aagcgtgtaa tttcatttct
      181 gcgaatggat ttcatgactt cctctaccga gaaaagtacg ttatccatat ttttcaactt
      241 cttgaactca tcaatacacg ctattagagc gagcttctag ctatagacta acttctttaa
      301 cagaaaaccc ttcttccgtt accagtttta cagcggtaat tttaaatatt tcatcaaatg
      361 aacgtcttgg cataatccag atcttcgctt tctcttaatt tggtgtcaat tttagtataa
      421 cccgtccaac ttatttttta actaagtgcc cggtttttct tgacaactcc acccaaaagt
      481 tagatttttt ggtctaagtt ttagggtcgc ttcattacta gaattcaaca agagatgttt
      541 tttattttat ttaaattatg gggtcagttt ccatttgatt acaaacttct ttgttttctt
      601 tttttaaacg caataataaa ggttttaatt tcagcctttt taattagaaa atcaattttc
      661 ccaaattatt ttctttagct ttcttaacga tttctgtcgc aactaccaca tctaatacag
      721 ccgaaccaac tgttttgaag atcgtgattt gttctccact tgtccgaccg atcaattctt
      781 cattgatcag ttggcctagt tccccatgat agctgtctct ttgaatatac ccatcttgca
      841 gcggtgaaat gaagtcccca gcttctttta gaacaccgtc catggtatca aaaataacaa
      901 catctgcggc tttgattatt tctcgtggaa tttcacacat ttccggtgtg taagcaccga
      961 ctccattgat gtgcgcacct tttttgaccc actcagcaga gaatgtcgca cgttttgaag
     1021 tggtcacact tgtaatgatg tctgctcctt caacgcattc ttgactggtt tttgttgggc
     1081 gcatcgttac agaaaattgt tgcatcattt tttcagcaaa ttggctggca cgctcaaaat
     1141 caatatcaaa aatccgaact tcttctaatt tacgaacggt caacattgct tctaattgtg
     1201 actgtgcttg tccgccggtt ccaatcagcg cgccaatttt agcatcttct cttgcaagaa
     1261 gttctgttgc tgctccttga acagcgcctg ttcttaattg tgtcaaataa gtaccatcca
     1321 aacaagcgga taccataccc gtttctggat ccaatacgat catcgttgct ggtacacttg
     1381 gtaagttttt cttgatgttt tcaggataca cggaaacgat tttgacacct agcgcgcctt
     1441 cgccaccttc tacataagca ggcatatata agctttgtcc gttgcttttt ggcacatcga
     1501 tattggtgcg aagaggcaca cttgcctttc ctgctgaata caatgacagt gcttttttgt
     1561 ccgcatcgat ggcttctctc attgaaaaac ttttgataat atcttctttt ttaagaaata
     1621 acatttgaat cgctccttaa ttagctgctg taactttttt taattgactg cccaacatcg
     1681 tatatgcact gtatagtaag accaaaatca cgatccattt caggatatag gtatctaaag
     1741 atttgactag gaatacagcg accaataccc ccaatacacc gaaggttgaa gtaaaaagtg
     1801 tgatttttct gctgtattca ttaaatttaa tgaattgaac actgccgatc ggtactgaga
     1861 aggtacaagc ccccatcata ataggaaagg ctgcaactgg attcaagcct agcaagtaga
     1921 cagttaccat cgttaacgca taagaaccaa tgccgatatt gttcaatgca ccatagacaa
     1981 agagtaaaaa tcctgccaag attaattttc cgccatacaa ttcactggct gttccgtttg
     2041 atggaatcca gttcatttgt cctgcaaaaa tcaacaatgc agcaatgacc aatcctatgc
     2101 cgacaaacag tttgatcgtt ttctctggta acttcacgac aaatcttggt cctaaatatg
     2161 caccgataat ttggcagata atacaaacag ctagtgtctt gatccctacg ttgatggcag
     2221 tgatgtaaga caatgccatg acggctaccg gtactacaca ttgggtattc aacgttccgg
     2281 gtagtttttt catgctgacc catttcaact ttggataaag gacggttccg atagcaaagt
     2341 cggaaacgcc aaatgttgaa agaaagaaga tgataaatga cgtgaagggc aacagacgca
     2401 tatcccctgg ttcttctttg aaattttgtt tgtttttgaa aaggtctctt agaaagtaaa
     2461 taataaaata tagattgatt gcgacaatta gtgtcagcaa aatattccga atcataaaaa
     2521 attcctccta ctagtaaatc tttctgattc cctcaaagcc ctcaccatat tttgctttaa
     2581 gttcttcagg agattctgca atcactttta gtgtatatgc atcatgtttg acatattctt
     2641 ttgctagatt gaccatggcg tggttttttg tccaagtgac atcggttgat tgcagttctt
     2701 cgttaatggt accaaacacg acttcttgct catcggcgac gatgttgatc catcttgctc
     2761 caaagtcagc aagtttgtct gtttcaaaac catgaatata atacttttct aagggaagat
     2821 catattcatg ggtagaactg aataaaatca gtacaaactt atcaactctt ttttcagctt
     2881 gctgcaatgc ctttagtagt gaatccgtga aattctcatg ccaaacttga atgagcaaac
     2941 tttcttttgc gtttttcacc aaatgctcta ctttatccag aacatactcg ataccatcaa
     3001 ctttccacag catttcttct tcatcttttt ctttgatcat ccctagattc ttggtaagaa
     3061 aagaaagatc atttttaaca cttttttcta atttctctaa aaactcattt gccgagatgg
     3121 catgataaag tttaggttct gatttattaa cgacaatcaa attctttttt aatagttttt
     3181 ccaaaatatt atatactttc gagcgtggca cacctgagat tttacttact tcatatcctg
     3241 taagattacc tttttctaat agtgtggtat acactcttgt ctccatctct gaaaaatcat
     3301 attttttcat gatttgaata atcgtttcca ttgattaatc ctcactttat ttttagtagc
     3361 tactacggta acaactataa attctttttg attatctgtc aagagttagt tcaataacac
     3421 acatataata attagcttaa aaacaatctt tttattaaaa agaaacatta taatatgaat
     3481 gtttgtgaaa aaataataca acttagtcat tctttacaac gatttataga aaaacaacct
     3541 ctttaaatat aaaaggtacg ggttactgat tacttgtaaa tctttacgtt ccagttttat
     3601 tctttctaaa aaatatactc acttcttttc ttagacttct ttttttgatt ctcctcttta
     3661 tggtcttgtc cttttttgcc ttcaaaatat ttaaataaac ccctttcaaa agcatttcta
     3721 ggttttggac aataaggaat gacttttttc ttcatataaa tacctcattt aactatttga
     3781 tatcatcaca ttatcaaaaa aaataggttt tttattaaaa ctatcttgac ctttcgggag
     3841 atttcactgt gatttatttt tattcccata cttttttcgt tggtcacttt cgtataccta
     3901 atctaagcga tcatcggcga cggtgctttt acaacgaatg gcaggacaaa acaaggggcg
     3961 gaaaaacttt tttgcctatt tcataaaaaa ctttttccta cagcaatccc ttgttttgtc
     4021 ctaccacacg ttgtgaaccc aaagcacgcc gacgaacgct tagctatacg aaaatgacct
     4081 ccaaaaacaa aaaagtgagg tgaatagaaa caaaaagaaa ttgaaatctc ccaaaatgtc
     4141 gtccgcctcg gagacaactt gctgctgaaa atggttggtt aattcattta agtattaaat
     4201 aagataaatc atcaaattaa tcggaaaaat agcatgattg gccttatcaa aaataaactg
     4261 taaaaactta tatttctatg agtggtggaa caatgctaat accaaattaa tttatcgatt
     4321 acccacctgc acaaaccaaa gatagcatta ttaggaaacg aatcaatcag cgaatccagc
     4381 attataatga ggatactaaa aataaagttt tcataattat aaatccgcct tcctacgaaa
     4441 ggcaccaatg aggtgatgaa aaccttagag cgctttcttt ctttttttat tccgtgttac
     4501 cctttccttg caggtccctg gtacactaca gaacccgagg aggtgagtgg cggggctgta
     4561 tagcggcaac cccgcataat tatatggtgt cggtcatccg ttaaggcatc aatgaatggc
     4621 gcaaacccgt agcccgtaaa cttatctctt ccgaagttga ctcgatttcg ccggatgacc
     4681 agtccctacc aaacctgtac tattttttta caggaggttg ctatgttcaa aatctttcgt
     4741 aaaaattgtt gtggcttgga tgtccacaaa acgtggatct atgcgtgtat cggaatcaca
     4801 gatgcgaatg gtcgtacagt gtataagcaa gctcgcttct cttcctttac aaaaggtctc
     4861 aatgaattag ctgaatggct tgctaaatac aactgtaagg atgtgtgttt ggaatcttcc
     4921 ggtaaatttt ggattcctgt ttttaatatt cttgaaaaaa cttgttttgt cacactggct
     4981 catccgaagt acaccaaacc ccagaaagga aataaaacag atcgcaaaga tgcaaaaatg
     5041 gatttgtgat ctctttatgt gcgacatgat caaacccagt tttattcctg gtcttgaaat
     5101 ccgccagctt cgtgatctaa tgcgttatcg gacgaaactg accaacatga aaacaagtga
     5161 aaagaatcgt gctttaaact gcttaaccgt ttctaatctg aaactagatg atgtttttag
     5221 tgatgtgttc ggaaaatcat ccacttctat catccaatac ttgttagaac atcccggtga
     5281 atcatttgat gttcgaccat ttatccatgc ccgttgtaaa actcccgttg aagaaatcca
     5341 agcggcagtt gagggggcga tttctgtacc tcaagcgatt aagcttcgcc aatgcctcaa
     5401 ccatattaac gagattgaga agcatcgaga agagatcaat ctagagattc aagctcttgc
     5461 agaacccttc tcccctgttt tggaactact tcgtaccata ccgggcttag acactcagcc
     5521 aataacagcg attgcgattc tttcagaaat aggaccggat atgtccgtgt ttccaacttc
     5581 gaaacacttt atttcttggg ctggatgctg tccaagaaac gatcgcagtg cccgacgaac
     5641 aaaatcaaca cgtatctctc gtgcagggca gtcgttgaaa ccgctacttg tacagattgc
     5701 caatgctctt ctcagatcca aagaacatcc agaatttaaa aatcgctatc gtagaatcaa
     5761 agcgaatcgt ggacacaaga aagctatcat tgctatttgt aagatgctcc tgaccgctat
     5821 ctggaatgtc ttatcaaagg tggaacccta ttctgctaaa ggatatctgg agccaaaacc
     5881 tgcgaaagaa gagaaagttc tgacgcaatc ccaagctctg gaccttttaa gaaaacgcgg
     5941 atataccatt atagatagtg cttaaaccaa agttacgttg attcttccta aatagactcg
     6001 ttgtcttcca tttcaaaaat tttgaaaacg gtaggcttat ttagtgtacc gttcttcagg
     6061 tatcttcaaa tagatttatt ttcaaactta tttcctttct tcttttactc tcctatagct
     6121 tagcatttaa gtgggcactc gtgtcacctt gctagatagc tatgaatgcc gtaataagcc
     6181 tcctagtata taggcagact gccaattata actgattcaa tccacattat gataaaatcc
     6241 accttttcgt ttcatatgtg ttgaaatatc acatatgtca aaagggcacg agtcttttca
     6301 ttcagtgctt tgtaaatact caaaattatt ccttttcact ttaaaaaaaa gataattagt
     6361 acaaaatcag aaatgaggaa agtattagga tggactagaa aggagttgtt gaatttgttg
     6421 caacagattg gtcatttatc aattaaaaat taggtgaagt tttattcatc actgcagctg
     6481 ctgtttccca gagaataaaa cgtctagaag aaactggtca gttactgtat tatcaagcag
     6541 caattgacta cgaacaagct tgattactaa ccaaagcgtt tatccacctg ttgattaaat
     6601 caaatcaaaa atcaggtttt tatacgttca taaaagcaat ctccagtgta ctcgaatgtg
     6661 actgtatcat aggtccttat tcgatgttaa tcaaaatggg gtttccttct accgaatcat
     6721 tggacagttt tatcaatgat ttatcaaagt ttggacgaac caacacccaa atcgttttct
     6781 cgactcttgt ttccttaatg gtttatcagt tctggaatgt ttatcatgcg cttttattaa
     6841 aatagaaaag agcttttttc atgcgacaaa gcagtgatca tttattggca catgcaatct
     6901 ttgtggatcc attttttgaa aatacgatat ttagttttga cccaaaaatc tttaaatacg
     6961 tatttga
//
LOCUS       DACDRN010000102         6711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDRN010000102 DACDRN010000000
VERSION     DACDRN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.102"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(49..873)
                     /gene="iolB"
                     /locus_tag="IWJ80_002570"
     CDS             complement(49..873)
                     /gene="iolB"
                     /locus_tag="IWJ80_002570"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HAP9462248.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(912..2828)
                     /gene="iolD"
                     /locus_tag="IWJ80_002571"
     CDS             complement(912..2828)
                     /gene="iolD"
                     /locus_tag="IWJ80_002571"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HAP9462249.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2839..3873)
                     /gene="iolC"
                     /locus_tag="IWJ80_002572"
     CDS             complement(2839..3873)
                     /gene="iolC"
                     /locus_tag="IWJ80_002572"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HAP9462250.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4030..5046)
                     /locus_tag="IWJ80_002573"
     CDS             complement(4030..5046)
                     /locus_tag="IWJ80_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9462251.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5148..5345)
                     /locus_tag="IWJ80_002574"
     CDS             complement(5148..5345)
                     /locus_tag="IWJ80_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462252.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(5317..6636)
                     /locus_tag="IWJ80_002575"
     CDS             complement(5317..6636)
                     /locus_tag="IWJ80_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011674482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAP9462253.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
BASE COUNT         1771 a         1635 c         1158 g         2147 t
ORIGIN      
        1 aaacctaact aaacctaact aaactttatg tatctaacag cttaataact acttgcgctg
       61 caagatccat tcgtgtttct tttcattgtg gaatttccaa attcgctcag gtcccgccat
      121 cacatttaaa tagtaggaag tatacccgtc aggtacccca actggatgat agcccttagg
      181 aacttcgacg acattttgat gctctaccgt catcgtttca tctagtgaac gatcttccgt
      241 gtagacccgc tggaaaacaa atccctgctc tggatcaagc tcatggtaat aagtttcctc
      301 taaaaaagac tcatgtggca gattctcttg atcgtgctta tgaggaggat agctggaaaa
      361 attaccgccc tcggtataaa cttcgacgac cagcaggctg ctggcagtat cgacattatc
      421 cggtaaaatg ttgtgaacaa gtcgtttatt ctgatagagt ccacgttctt cgacggtatt
      481 atcctccgcg cgaatcacac ttatcggcaa agcttttttc gctggtgaat agcaaagcgc
      541 caatcggaca ttgtctgtag ttgccacaag ttcaaacgaa tgctccgccg gaagataaat
      601 actgtcggtt ggaatttttt caaaaacaga ttctcgtgtt cctaaatctt cataccaagt
      661 gtctgctgct ttaaccttaa tttttccagt caaaatgacg atacagacct cctgttgact
      721 gccggtttcc ttgtaagaag ctcccttttc cagctccaaa atctgaaatt cgatataact
      781 caaagcagaa ttagactgcc gaatattttg gatttgtttt actccaggtg ccagttcttt
      841 gtttaatggt tgatgcttca atgtcttttc caatcaatcc actccttatt ttcaatcaca
      901 aaaagcaata tttaatactt tctgatagtg gcaagtttcg cttgtttggc tttgtaagcc
      961 tcctgcatgg ctgctttacc cgatacctct gagataccga cgttccacca gctgccgtcg
     1021 tagccttctg tcatggtttt aggaagtacc tttatctcaa tcaacgtcga tcgcgtttgc
     1081 ttcttcgcat cctcgaccgc tgcttgtaat tctgcaatcg agcttacacg atacgttttt
     1141 gcaccatagc cttcagcaac tttggcgtaa tcgatgttca tgatctcgtc cttggtattt
     1201 ctgaattcgg ttccttggct agctccgcca ttatccatct gtaaattatt gatacagccg
     1261 aaaccagaat tatcgaaaag tacaatgttg atttttttat catattgcaa actagaaacc
     1321 agctcgctat gcaacatcaa gaaactgcca tcgcctacta aggcatagac ctcttgttct
     1381 ggtcgagcca ttcttacgcc catcgcacca gcgatttcat agcccataca agaataaccg
     1441 tattccaaat gataggtatt ctccttcaca gggttccata agcgctgcat atcccccggc
     1501 aatgaaccgg ctgaaccgat cacgatcgca tcctcatcaa caaaatcatt taaatgaatg
     1561 aagacctctg tttgcgtcaa attcgtagcc aacgcatcag catattcatt caagatttct
     1621 tgagaaaaat gatctttgat ttctggcgta aaatctttac gcttaaaatg cgcggctttt
     1681 aaccgatttc gctcactgat ccattcttcc ttcagttcat cgatcttctc gccataggcg
     1741 gtttggtaac cttcgatcat tggggcaagc tgctccaagg tcgctttcgc atcaccaacc
     1801 acttgaaagg cttccagctt agcggcctgt tgtctgctta cgttaatatt catcattttc
     1861 gcttgttcaa aattaaaaat acttttagaa gctgaggtaa aatcagtgta gcgtgtgccg
     1921 atccctatga tcagatccgc ttgctgcaca gctttattag cagccgaagt tcccaaaatc
     1981 cccattccgc cgagattgtt tttaaaatcc cacgaaacag tcgatttacc tgcgtgcgtt
     2041 tcaactagcg gaatctgaca atctcttgag atttgctcaa gaatctcacc agcctccgaa
     2101 tatttgcagc cgcctcctac gatgatgaca ggtcgtttac tggcctttat tcgttgactc
     2161 gcttcagcaa gctcacgagc ggtcggaatc ctgcgatcaa tataatgtac tctttttttg
     2221 aaaaattcct ccggataggc ataggcttct gcctccacgt cctgcggcaa acaaatcgtt
     2281 gccggaccca tcgttgccgg gttcgtcatg acttcaaagg cccgcaataa cgcggacatc
     2341 aactgctctg gtcgattgat gcggtcccag tagcgagaaa ccgcttgaaa tgcatcattt
     2401 gtcgaaaagg ctacactgct tgcatgttcc agctgctgca agaccggatc aggctgacgt
     2461 gtagcaaacg tatccgccgg taaaaaaagt acggggatat tatttgcgaa tgccgtaccc
     2521 gctgctgtta acagattggc cgcgcccggt ccaacagatg ttgatacggc aaaaattttc
     2581 ttgcgtaaat tctgtctggc aaaggcagta gccgcatggg ccattccctg ctcatttttt
     2641 ccgctataac ttttcaaatg tccgggctct aattccagcg cctgaccgat ccccaaaaca
     2701 tttccgtgac catagacatg aaagatacct tcaacataag gcgtttcttt gccgtcgatg
     2761 ctgatgtatt gttgattcaa aaattttagt aatgcttgtg ctgttgttaa acaaaccgtc
     2821 ttttccattt ttttcacctt aaatccgagc gatcatttcg ccgtatattt ctttctccaa
     2881 tgtaataaaa tcttttacct cctgagtcgt cggtaaagaa tctgaacagt tattgctttt
     2941 gaccatcatt gatgcttccg ctgagccaaa ctctaaacaa tcgatgatct cccagccttc
     3001 gaataaaccg tataagaaac ccgagccgta accatctccg ccgccaaagc ctttgcgtgc
     3061 ttccaccgga aacggcttga ttgaaaactg ctgctgatcg gccgtataag cagtcgatcc
     3121 tttcattcca tgtttgatta cgacaatctc agcaccacaa ctgtgccaaa aagcagcgct
     3181 ttcattatct gacatgttag gctggatcaa cttctcagtt agatcgaact cttctcttga
     3241 acccatgata atgtctgatt ccttagcaat catcgaatag taaattgaaa tatcatcgtt
     3301 gttcttccag ttataagcac ggtaatcaat atcaaaaata attttgacat tattcctttt
     3361 cgccagcatc actgccttga ttacggcttc tctggacggg ctctctgcta gtgcagttcc
     3421 tgagattaat agtgcttttg accgtttcaa gtagtcctca tcaatatcct caacgctaag
     3481 ctgcaggtct gcggcttgat ttcgatacat caaaatatta ctttcctttg atgagagcat
     3541 ttcggtaaaa gtcagcccca gcttttcacc atgctgcgct ttgctgatct gagacgtatc
     3601 gatgccttgt tcattgaaat aatcgatcac atattcgcca aactgatcgt ccgagactgt
     3661 tccgataaaa ccagctttca tgccatgttt agtaacaccc acagcaatat tggcaggaga
     3721 tccgcccaca aatttttcaa acatatggac ctttttcaat ggtttaaact cttctttgac
     3781 ttgatcattg taggcaggat taaaatcaat agcgactctt cctaaaagca gcaagtcaaa
     3841 ttcttttgat tggtcaaact ttatataact caatatatcc acctctttat ccgttcttct
     3901 atttctgagg caagtatagc acgccaaaac aaagcgtgca atatttattt tagaacgcaa
     3961 tattgcgtgc tgtttgaaaa tgacggcaaa aaaaacgtga atcgccttac atcacgattc
     4021 acgcctagat taaatattgt attttgtctt aataattata tcagtaaata gcgcatcttt
     4081 tgtaaccgtt ttattatttt gcagctgatt cgctaacatc agtaatgaac gatagccttg
     4141 aacaaatcca tcttgatcga ttagaaaatc aacctgatcg tttttcagca tctcgatatt
     4201 gttcttagtc aaatcataaa tgattacaaa gggccgcttg ctttcatcga ttttttccaa
     4261 cgctcgggca attcccgctt gtccgccaga aaagacgact acgccatcta agtttgaata
     4321 attctccaag gtgttgctta aaatcttttc aacctcagca ctcgaatcaa agctgctctg
     4381 aacacccgtc agctccagtt caggaaacga ctctttcaat tcttcaacaa agcctgccac
     4441 ccgtaagctg ttgacgctat ttccaaagta gccggtaatc gctagaacct tgccctttcc
     4501 ttttgtcagc atacctaaca atcccgcagc cgtttttccg cttttgaagt tgtccatccc
     4561 aacaaaacac tgtcgattcg taccaagaat atccgaatta aaggtaatga tgaggatgcc
     4621 ttgatcggtc aattgattaa ttttttctct gataccatca ctatctactg gcatgatcgc
     4681 gatccctttt actccaagct ccaccaatcg atccaacgcc tgcagctggg ccgcttcatc
     4741 gaccccgtct acctcttcta aaaaacaatc aatgtttcgc ttttttaaat ccccgataat
     4801 agattccaga cctgatcgga tcggaatcat aaaagaagat ttcgccaatt gagtgacaat
     4861 ccccagcttg agcgttgtct cttttatgac cttctttttc tttggaacat agttcatttc
     4921 cttggcgatt gctctgattc tttctgcaac ctcaggattg atccttcctc gatcgttcaa
     4981 tgcccgatcc actgtccccc ttgacactcc tgctttctca gctatttctt taattgtacc
     5041 cgccatcttg cctctccttt tttcctattg tgtttatttt atcacaaaac ttaagaaaac
     5101 caccggagaa tgaattctcc ggtggacttt ttgtatgatt aaccttctta ttgaacatca
     5161 ctgatcttaa actctattcc aagggcgttt ctataatatt ctggatacat ctccttccca
     5221 catttttcac aggtaaattg gggaggattg ttcggatcat ttgcaatatc tgcatcatct
     5281 aaaaatttta ctacatccat tgggattgat tctttttcac cacaacttag acatataaat
     5341 ttcacgttgg cgttcccaat cccagcgaat tgctttaaaa gttttttctt tttcttcttc
     5401 atactgttgc ctgaattctt tttggtcaat ttttcgattc tcctctcgca taaaccgctt
     5461 tgcatggtta tctttcgcat atttaataac taaccgtcca tttttgcgca cactcatccc
     5521 aacgaattca tagtactcac cgcaacaaga acattctaag ggattttcac caaattcttc
     5581 ggttattctt tcttcccagg attttggtct taaggcctca ttcagattga taaacagctt
     5641 ttttactcgt ttctgatact ccttcatgac ctttttcatt aatgttttga ttctacgact
     5701 atagattcca tagcgacgaa tcgttttgaa attcttattt tgaatatggc gaatcagtgc
     5761 agctatgaat tcgtctatac tcatggtcat cgtctcttta gtattcgttc gtttgtccat
     5821 gtagttgaac atgacagtgt cgccatcata cattaagatt cggcttaatg cgatcgggcc
     5881 acgcttcata taacgactga tgtattggag tagtgacttt agatttgtcc tgctcctttt
     5941 aggagcattt acgtaaaacc cttctccatt gttcttgtac gcagcttgta atcttgattg
     6001 aactctttgc ttgtcccact cacctaacgt tcgtctaatt agtttcaata cagcattttt
     6061 ccagtatttt cttaacatag aaaaaggtaa ataatcgtat tcttcccaac ttccatctct
     6121 tgttaagcca cccatagtaa caaccatatg aacatgcgga ttaaattcta gttttgatcc
     6181 aaaggtgtgg attgtagcta ctatacctgc cttcaccttt cttttctcaa aataggcaag
     6241 aataattttt gcggcttcat ccattagtcc tttcagtaag gaatctcgat agttttccat
     6301 cataaagatt tcccgtaacc cctcatcgat cgtaaagatt acgtgccgat gtgtcacttt
     6361 aaacatatca ttcgccacaa ccgctgacca cctttggctt tcacctgttg cacacgttgg
     6421 acagaacttt ccacgacatt tcaagggaac aaactttgtt tgatgacatc cctcgcagac
     6481 aaacagtcgg tatccttttt gaatatctcc acaatatcta aacttgttta cttcccgttt
     6541 gacgactagc cgaatctttc gcccgtattt ctcaacaaag ttatcccaat tatggtgttc
     6601 gtcaaaaaag attgcttcga gaatattcgc tttcatagta atattttact tgttcaaaaa
     6661 caaaatgaaa agggctaaaa gactggtagt gcagcctttt tagcccttaa c
//
LOCUS       DACDRN010000103         6511 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDRN010000103 DACDRN010000000
VERSION     DACDRN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.103"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(272..1855)
                     /locus_tag="IWJ80_002576"
     CDS             complement(272..1855)
                     /locus_tag="IWJ80_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462254.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALV
                     LDLIDKLSDLANIYEDITSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLPYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLKILKEEISKYRFTAESREEINKSLGENQQITYTK
                     GVFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGSN
                     NEELQNHLNSLLDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKNLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            1977..2561
                     /locus_tag="IWJ80_002577"
     CDS             1977..2561
                     /locus_tag="IWJ80_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462255.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSIT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTIISNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIDTSIKELREYVQNPYNFQYLAGRINLV"
     gene            2572..2817
                     /locus_tag="IWJ80_002578"
     CDS             2572..2817
                     /locus_tag="IWJ80_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462256.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLVLNKRKKDR"
     gene            2823..3545
                     /locus_tag="IWJ80_002579"
     CDS             2823..3545
                     /locus_tag="IWJ80_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462257.1"
                     /translation="MKSTKWKIVLLSTAALITLTACGTTNSADNNKDTKSSSTVVTKN
                     SSTSASTTISSMTENNAEKYQKYEDINKGQKPEEVYTDFINTVKNDEDYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYHAVAVDITDGV
                     PEWIQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            3676..4419
                     /locus_tag="IWJ80_002580"
     CDS             3676..4419
                     /locus_tag="IWJ80_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462258.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKAKSIRELKTEVEKIRLGLTREEISRLSENSSFVIQKEKEKHLLHRELIIEKYYDV
                     DLPDQLRIIDEFSEKKWKSEIYPSLIQYKASGKPMYNYTGFLNYTQYKYAFRKLKGIK
                     QADIALYEMKKMDRLKLIQELVQNTWNQAIVELEKENGKILTNEYLIVNEIEELIGLF
                     SCENGEVFLKSSPIYSILDSPYQFIQVRR"
     gene            4425..4790
                     /locus_tag="IWJ80_002581"
     CDS             4425..4790
                     /locus_tag="IWJ80_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462259.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDQQFNQTHHNIILLVDQAKRRLEKAEYELLDQGIICLDKGIRRYYSAELKKQ
                     NVDRHALKHEILELIMQRI"
     gene            4806..5336
                     /locus_tag="IWJ80_002582"
     CDS             4806..5336
                     /locus_tag="IWJ80_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462260.1"
                     /translation="MSQNIGAVKQLIEKDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAEEKSSILEISQ
                     NDKAIQVKCVDPTGKIIVGFDIERKSKIDFITQLTKINNLNILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFSNA"
     gene            5498..5866
                     /locus_tag="IWJ80_002583"
     CDS             5498..5866
                     /locus_tag="IWJ80_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462261.1"
                     /translation="MWNNKLKTGLLLIVISCAMMVGMIIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSNLMMKKV
                     RKLAQNYLEEKEGNSPIDKN"
     gene            6057..6317
                     /locus_tag="IWJ80_002584"
     CDS             6057..6317
                     /locus_tag="IWJ80_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462262.1"
                     /translation="MAYENLQLVVAEVSGDIYLTKVKDGIMDTNYRRVATDDVLSSST
                     EWFRKNEESAVHFEGIDGNIHSLFYTKDKEKAKKILDILKGR"
BASE COUNT         2433 a          847 c         1103 g         2128 t
ORIGIN      
        1 cacaccgaat gttaattgat tataactata aaaaaggatt gaacattatg ttcaatcctt
       61 ttttcaatga aatgttttcc gccatttcat tgtttggtgc gatttctatt ttattatatc
      121 aaaataaaga gagattctaa agaaagaaga gggaaatata taaatcaagc gacagtatat
      181 ttattattaa atgctattgt gagaatataa ctaattagtt taatttgaaa gtaaatatac
      241 gctataaaag tgcatcttgt gttataagaa attattttat tttgtgaaca taatttaaca
      301 attctttctc ggaattgatt tcttttctta aatagtcttc agacttatta ataatattat
      361 ctattatgtc tagatacata tcacttttcg attcagctaa ttcatacagg ttttttaatt
      421 ctttatcctt taattgaaaa ccctgatcca tagtcactgc atttggatat agaagcccac
      481 tataatgttt acgtaaattc acttggaatt tctgattata aaagtctaac agattagaaa
      541 taaaaggcgg aaaattactg tttttgtaaa attgattatt tccatttagg ttgtatagta
      601 atttgttaaa ttgctcctga tttaatttat tgtaaatcca tggtaatact tccatatcct
      661 tatctgctaa cctgaatgaa ctagcatcgg taagatgttc gatcataaaa tctatgaaca
      721 tctctttttc accaaaatat aaatattgat tatatattgt ttctaatgtt gaagatggta
      781 gcgaagaaac tctcattttt tgctgaagta taggatcaag tagactattt aagtgatttt
      841 gtaattcttc attgttgctt cctagaactg caagtgaacg taaacggtag taatattcta
      901 tttctgttag tacttgttgt ttcggtttaa aatcatttat tgatatttgt aaattttgaa
      961 ttgtctcgtc tgaaaaatca tgttgtaatt ctggatgggt agctataaaa gtaagtaaaa
     1021 agaccccttt tgtatatgtg atctgttgat tttcaccaag tgatttatta atttcctctc
     1081 ttgattcagc tgtgaaccta tactttgaaa tttcttcttt tagaattttt aaatagaatt
     1141 ctttattatc aaaaattagg gcattaagta gtctgaaagt actttcccta tctctatctg
     1201 cttcctcatt tccataaggt aattgaaaaa caaacttaaa ggctgtctta cataggtttt
     1261 cttttgcttt ttcgcttagt ttatttagat agctattttt tagtgcagag tgaaattctt
     1321 caggggtaac agacatagca tgtatgcgac ttgcatattg tataatttcg atcgttactt
     1381 tttttcctaa tattggattt tttaggaata taatttcata ggacttccgt ataacatcct
     1441 gacaagtttc ttttgttata tctcttaatt cccaagtatt accgttgtaa gtgactacag
     1501 gatgagctga ataatttctt tgatccttaa tatattttaa atcctcatat tcatagctat
     1561 caattagctc agttgaacta taagcttttt ctagtagttc catttcccat tttggagaat
     1621 ttggatcact ttttcttaat ctagctatat cttctaaaat tttcttagaa gtaatatcct
     1681 cgtaaatatt tgctaaatca gataatttat caattaaatc taaaacaagc gcactgtaat
     1741 tgacagctat tgcagaacga taatttccct gataaaaact acttcgaact tcttctaata
     1801 atggaataat tctaggattt ttaacttttt ttatttcatc atctaatata aacatataaa
     1861 tctccttttt ttgtttgtat gacaagttta ccataaaaaa atattaaaaa gcgagacaaa
     1921 tttatgttat tatgttatca tgatagcata aatatatgga gcgagatgat ggaaaaatgg
     1981 atttgaaaca atcgttttac atctggttat atcctgataa tcctatgtca ataatggctg
     2041 atatttttgt agttagcatc ttattgtatt tattttattt aattttcttt cacaatttaa
     2101 tcgggtacat gattcttgat tggaaaggaa tgttcgatat agagagaaaa ccatttgcta
     2161 acaatcaaac ctataccgtc ttcgaaagtg gagattggga agaaaatgaa gttgatgtga
     2221 tccatgaaat tggattagct ttatcaggaa tacaagatgc tgatgttttg gtatcaataa
     2281 caacggatac tggatacaac gcttatgagg tattttatct tatgtatttt atatggcgtg
     2341 atggtcaaga aattatcaat ggtcgtgctg aaaaaacaat catttctaat actgaaattt
     2401 ctttctgtat gggtgaaggg gaaatgattg tatataaaga tgggtcagca aacattaaaa
     2461 cagcctttgg tgatattgat acaagtatta aggaacttag ggaatatgtg cagaacccat
     2521 ataattttca atatttagca ggaagaataa atttagttta ggagtaaaaa tatgaaaaaa
     2581 attattaatc aaccaacaaa tcacttgata attgaaggtg ttattttcgt aacaatgtat
     2641 tttactgtag gaaatggcat ctcaaataca atcattcaaa aaaatccaca atacctatta
     2701 tttagtcttt atgcttggat tgtgcaaatg gtttgggttt ccatattgat gctgttgttt
     2761 tattggatag tacgggttat tcttttagtg ttaaacaaac gaaaaaagga taggtgaata
     2821 aaatgaaatc aactaaatgg aagattgtat tgttatcaac agcagcatta ataactctta
     2881 cagcatgtgg tacaactaat agtgctgata ataacaaaga cacaaagtct agtagtacgg
     2941 tggttactaa aaacagctct acatcggcat caacaacaat ttcttcaatg actgaaaata
     3001 atgctgaaaa gtatcaaaaa tacgaagaca ttaataaagg tcaaaagccg gaagaagttt
     3061 ataccgactt tattaataca gtaaaaaatg atgaagatta tgggaatgat ccattgcaaa
     3121 ataaaaatct gactatcgtt ctttacaaac ctgattgtcc agattgtcaa aaagcagaat
     3181 caaaaatcat gcaagaaata aaagaaggat ttagttatgc tccaacatta atgcatgata
     3241 ttaccataaa taaaccagct tacgagtatc acgcagttgc tgtagatatt actgatggtg
     3301 tacctgagtg gatacaaaac attcctggtc tggctgatga gaacaatcaa tatcatacgc
     3361 caacagttgc agtagtaaca ccaaagaaag ttaatggcac actatactgg gatgtaatgg
     3421 gaatgacaac aaatacaact gatgaaatga ttgaaagtac aactaaatat tatatggatt
     3481 tgggagcata taaattatat caacagtcag aacctgttat ggacgatcaa tccaaagtta
     3541 aatagttctt tagggcgtaa gccctatttt tttaaaatta agtgataaca aataaatgat
     3601 ttctgaaaca aacgttctta ctcaactact cgaaacacac aagctaatta actagattat
     3661 aaaggagaat gcgtaatgaa agaaatcagt gcaaatgaag ctcatattcc gcaaaagaat
     3721 caggttggta tatataaggt gcttcatggt gaaggaattt ataaaataca gttacttacc
     3781 ggttacaata acggtgcaaa tgggaagtgg ttaaaggcaa aaagtattcg tgaacttaaa
     3841 actgaagtag aaaaaataag acttgggtta acaagggaag aaataagtag actgagtgaa
     3901 aattcttctt ttgtaatcca aaaagaaaag gagaaacatc tacttcatcg ggaactaata
     3961 attgaaaaat actacgatgt tgatttacct gatcagttaa gaattattga cgaattttca
     4021 gagaaaaagt ggaaatctga aatataccca agcttaattc aatacaaggc atcaggaaag
     4081 cccatgtata actacacagg ctttttaaac tacacacaat ataaatatgc ctttcgcaaa
     4141 ttaaaaggca ttaaacaagc tgacattgcc ctgtatgaga tgaaaaaaat ggatagattg
     4201 aaattgattc aagagttagt gcaaaataca tggaatcaag cgattgtcga actagaaaaa
     4261 gaaaatggga aaattttaac taatgaatat ctgatcgtaa atgaaattga agaattaatt
     4321 ggtttatttt catgtgaaaa tggtgaagtc ttcttgaagt catcaccaat ctattcaatt
     4381 ttggacagtc cgtatcagtt tatacaggta aggagatgag ttacatgtct agcttgaaag
     4441 atgactatat ccaagcaaaa caagagtatg aaaaaataca gatgttaaaa tctgagaaag
     4501 tcgaattatt tttaacacaa aaattagcag aggatcatat tgctaactta gatgctaaaa
     4561 ctttagagga atatgatcag cagtttaatc agacacatca caatatcatt ttgcttgttg
     4621 atcaggcaaa gagaagactt gaaaaagcgg aatatgaact attagatcaa ggaattatat
     4681 gcttggataa agggattcga agatattata gtgcagagtt aaaaaagcaa aatgtagaca
     4741 gacatgcgtt aaaacatgaa atcttagaat tgataatgca acgaatttga agggatagaa
     4801 agtaaatgtc acaaaacatt ggtgctgtaa aacaattaat agaaaaagat ttagagacga
     4861 cagttcagag gttagccgaa cgaatccatt ttgcaaatta tcaaaccatc tctaattggt
     4921 caagcaggaa cagaaatccg gaagaaattc caatgaaatt tttttatcga atcggaaaag
     4981 aatatggtgt tgatccgata aaaattttcg aaaagaccat gatttattac tatcatggaa
     5041 cacctaacga agtgttggaa catcttttgg aagaagcaga agaaaaatca tcaatattag
     5101 agatatcaca aaatgacaaa gctattcaag tgaaatgtgt tgatccaaca ggaaagatta
     5161 ttgttggatt tgatattgaa agaaaatcta aaatagattt tattactcaa ttaacaaaga
     5221 taaataattt aaatatttta acacttagac cagatcatga acagttgttt atgagtttta
     5281 aaaaacaatt gaaagaaata caaaaaagat ataacaaggt gttttctaat gcgtgaaaag
     5341 ctaaaagaaa tcagagtgtt ttggataatt aacaaagaat ttattatctt gtatacactt
     5401 ggatttatca gcatgatctt gttttggtgg ttatatttaa aaaagtaatt taaaagtgtc
     5461 aaggagattt ttatccagag aaggatgtgg aatttgcatg tggaataata aattaaaaac
     5521 aggcttgtta ttaatagtta tttcctgcgc tatgatggtt ggcatgataa tacaaaggga
     5581 acaatcttat tttgaagttt ctgctaacaa tgtaatagaa agatgccatt atggtcaaca
     5641 tttttggact ggggaagtca gagaaaatat agatcgtgag tatgttcaaa gaatcgtttg
     5701 ggatgcatat tctatcaaag attatcctaa aagtttaacg agtagattat tttacagcga
     5761 aaaagataat caaaaactct cgaatttgat gatgaaaaaa gtaaggaaac ttgcacaaaa
     5821 ctatttggaa gaaaaagaag gtaatagtcc aattgacaaa aattgatgac actgcaaaaa
     5881 aatatagagt tcgaattagc ttttttcgga gcattgtttt ttaggtgaac tatatttcat
     5941 cactaaaatc agagataact aacttgaaac atggtcagat gtgttacttg aagtctctga
     6001 ataatcaatg ttatctagta gaattaagca actaaaaaag aaagaagtaa gaaaatatgg
     6061 catacgaaaa tttacaatta gtagtcgcag aagttagtgg ggatatatac cttacaaaag
     6121 taaaagatgg aattatggac acgaattata ggagagttgc tacagatgat gtgctaagct
     6181 catcgacaga atggtttcga aaaaatgaag aatctgctgt tcactttgaa ggaattgatg
     6241 gaaacattca tagtttgttc tatacaaaag acaaagaaaa agcaaaaaaa attcttgata
     6301 ttttgaaagg aaggtagtta attaaccaat gatataagag aatgtgagac caagtaaata
     6361 cttggtctct ataattttag ccactagtca attaaatagc taaacaattt taataataat
     6421 aaaatatgac tgcttcataa ttaagaagtg cagaattttt ctaagaacga tatttccttg
     6481 ttgaaatgag tattgctaac aatttgatca c
//
LOCUS       DACDRN010000104         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDRN010000104 DACDRN010000000
VERSION     DACDRN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.104"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IWJ80_002585"
     CDS             725..811
                     /locus_tag="IWJ80_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9462263.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IWJ80_002586"
     CDS             complement(932..1621)
                     /locus_tag="IWJ80_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462264.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="IWJ80_002587"
     CDS             complement(1866..2780)
                     /locus_tag="IWJ80_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9462265.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IWJ80_002588"
     CDS             complement(2762..3142)
                     /locus_tag="IWJ80_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9462266.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IWJ80_002589"
     CDS             complement(3592..3879)
                     /locus_tag="IWJ80_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9462267.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IWJ80_002590"
     CDS             complement(3899..4123)
                     /locus_tag="IWJ80_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP9462268.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IWJ80_002591"
     CDS             complement(4763..5296)
                     /locus_tag="IWJ80_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9462269.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IWJ80_002592"
     CDS             complement(5317..6159)
                     /locus_tag="IWJ80_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9462270.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACDRN010000105         5998 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDRN010000105 DACDRN010000000
VERSION     DACDRN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.105"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..450)
                     /locus_tag="IWJ80_002593"
     CDS             complement(<1..450)
                     /locus_tag="IWJ80_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9462271.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            673..1464
                     /locus_tag="IWJ80_002594"
     CDS             673..1464
                     /locus_tag="IWJ80_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9462272.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1430..2383
                     /locus_tag="IWJ80_002595"
     CDS             1430..2383
                     /locus_tag="IWJ80_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAP9462273.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2781..3755)
                     /locus_tag="IWJ80_002596"
     CDS             complement(2781..3755)
                     /locus_tag="IWJ80_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9462274.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3870..4304
                     /locus_tag="IWJ80_002597"
     CDS             3870..4304
                     /locus_tag="IWJ80_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9462275.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4429..5415)
                     /locus_tag="IWJ80_002598"
     CDS             complement(4429..5415)
                     /locus_tag="IWJ80_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAP9462276.1"
                     /translation="MEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGAFFRVHHFAPQIG
                     SPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQGRVQLGVGRGSPE
                     QVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPMFPQGSGKLRIEP
                     YSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQQANQLRAYKEAW
                     KQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYHRNPTIFGKTFAG
                     EPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIAPELGWR"
     gene            5843..>5998
                     /locus_tag="IWJ80_002599"
     CDS             5843..>5998
                     /locus_tag="IWJ80_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAP9462277.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIKIMA"
BASE COUNT         1888 a         1131 c         1034 g         1945 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgcttcaaa agaaatttat tttgttaaat ttgatctact tgaaaataaa
      601 atcgtttatc tatataatta agaagaacaa agtcgaacaa aaattgactt ttatgttaaa
      661 ggagtaattc atatgctgaa aaaagaaaga caagaacaaa tcattacatt attagagaaa
      721 gatttttatt taactaattc agaaatcgct aacaaattag gcgtatcaga aatgaccata
      781 cgccgagatg tcactgaatt agcaaaaaaa aataaagtaa aaaagctata tggtggcgtt
      841 gaacgaaagg aaccttataa tcgagaacta tcgactcaag aaaagattga tacgaatgta
      901 gccgaaaaaa aatatattgg tcaagtcatg aacagtatga ttcccgataa ttctactatt
      961 tatgtcggtg ctggaacaac gatcctatat gcacttcctt tcattcaaaa gaaaaatcta
     1021 tttgtaatta caaacagttt gattgctttc aactatttaa agaatcatac aagttatcgg
     1081 attttactta ctggtggcga attttctcct attacagaag agttcattgg ggaagttgca
     1141 gaaagatctt ttgaaagttt gaatgtcgat atctctttcg ctgcaacaaa cggcatattt
     1201 gataacaatg tgacaaccgc tcaatttatt gagggaaata ttcagaacgc agcgctcaat
     1261 gcagcaaaaa tcaaatgcat tgttgcagat tattcaaaat taaataaatc agacgtctat
     1321 acttttagaa aattaaccga ttttgattat ctaataaccg ataatcattt atcagaacag
     1381 gatttcaatc attattcaaa atacttaaca atattaaagg agcccaaaaa tgatactgac
     1441 aatcacgatg aacccatcca ttgattccat ttattttaca aaacactttg aattaggtac
     1501 gatgacacgc tttgattctc cggtacgcta tgtaggcggc aagggaatca attgcggacg
     1561 agcttctgct ctactaggtt catcagttat tttaacaggg tttttgggcg gttattttgg
     1621 cagtatgatt caagatatgc tgaaaaatga aaatttgttt gatttgcatt ttttacctat
     1681 tgcagaagaa tcaagaggtg ccgtcacgat catgcatgat gatgaaacgc agacagagct
     1741 agtggaagct ggtcctgaaa tagcagatac tactatcaaa aaattaatga atgatatcaa
     1801 actaatcagt aaaaataatt acctttcagt cattacaata aatggttctg ttaacaatcc
     1861 agatcccttt ttgtatgcta aattattaca atatatcaaa gaacaaatag atccttccac
     1921 ctatatatta atggatatat ctggacaaca tctccgtgaa gtcttagcaa gtaagtataa
     1981 accgatgttc atcaaaccta atatccatga gttttcagat atcatccatc aaaaaattac
     2041 tcaaaaaagc gacataaaac accatctaaa aacaaatcca ctatttaaag atatcgatgt
     2101 tatcatgatt tcttgcggct cgtatggtgc agtagtcaaa gtaaaagaag aattttggaa
     2161 tgttgagatc cctaagattc ctatcattaa tacaacaggc tctggggatg ccactgttgg
     2221 aggttttgct cattcattag aacagaatta tcctttaaaa gaatgtgtaa ggtacgcaat
     2281 ggcttgcggc atgtcgaatg cacagcaatc agaagtcggg aaagttattc cagctgatgt
     2341 aaaaaattta gcaaaaagca ttaaaatcac agaaatgaaa taaatagtac aaagtaaata
     2401 attgtttata tgaaatgaga cacaaagaga ctgtgacaaa aaacgtgtgt accgaccccc
     2461 aaaagttaga ttttttggtc taacttttgg gagtcgatac attgtcacag tccttttttc
     2521 gaataaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg
     2581 agaagctatt ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacgacct
     2641 cttggtataa acggtgttca aaagcttgca tctacgctaa tgagtccaac caagtcaaaa
     2701 atgaggagct tttttggctg tccttattac ttgatgcagg gcagtttttt cataatctct
     2761 tttagattaa atccttttgg ttacacaagg ttctcttcaa agaatgttgc cattttttca
     2821 aatggaatga tgtctgtctg gtcgtataga tctgtatggg atgctcctgg aatgatcatg
     2881 agttccttgt tgtcaccttt tagttctttg aaagcgtcct ccccaaagta acgtgaatga
     2941 gctttttccc cgtgaatgac taagactgca tgacggattt ctggaatata atgcaatagt
     3001 tttgcattca taagtgacaa ccctgatgtc ctattccaac catcattgga gtttaaagaa
     3061 cggacatgat atccgcggtc tgttttataa tacgcatgat agtctttgac aaattgagga
     3121 gcatcttcag gcaacggatc aaccacacct ccagcacgtg cgtagtcccc ttgtttgaaa
     3181 tcaatgatgc gttgtgcatt taattcttca cgcatttcat atcgggcatt ttcatccata
     3241 acatcgaagt agccatttgc tgttactcga gtcatgtcat acattgtgga aacaactgtt
     3301 gctttgaccc gcgtatcgat agctgctgca ttcaatgcca tacctcccca tccacaaata
     3361 ccaatgattc cgattctctc agggtttaca tttttttgta cagataaaaa gtctactgcc
     3421 gcttggaaat cttccgtatt gatatcaggc gaagcaacat atcgaggtac tcctccgctt
     3481 tctcctgtaa acgatggatc aaacgcaatc gttaaaaagc ctctttcagc catcgtctgc
     3541 gcatatagac cagacgattg ttccttcact gcaccaaatg gcccgctcac agcgattgcc
     3601 ggcaatttct tttcacttac ttcttttggt tcatataaat cagcgactaa tgtgatacca
     3661 aaacgattcg ggaagctcac ctttcgatga ttgacttgat tgctttttgg aaatactttg
     3721 tcccattctt gtgtgatgtt taattcttta ttcatattct agactctcct ttgatcaaaa
     3781 ttgacatttg tttttgaatg tattattatg ataaaactta aacttgactt taggtcaagg
     3841 taaaaatttt attttaggag gattttttta tgtattccat tggacaagtg tctgaaatgt
     3901 ttggattgcc tgtttctact ttacgttatt acgacaaaga gggattattt cctgatatcc
     3961 agcgtgtttc tggtattaga aaattttcag agaatgagtt agaggcttta agagtcattg
     4021 actgtttgaa aaaatctggt ttagaaataa aagacattaa gcaattcatg gaatggagca
     4081 aagaaggcag taagacattt gaggttcgta aagaactatt tgaaaaacaa aaagaagtag
     4141 tagaaaaaga aatagcaaaa ttagaaagag tactcgctat gctaaactac aaaagctggt
     4201 actatgaaga agcaataaaa gccggcaatg aagaagccgt attgacaatg attcctgatg
     4261 atttacccca acatgtaaaa gaagcttatg tacattctca ttgaacaaac ggataactca
     4321 ctctccttct ctatgaactt catgaaaaca tgaataaaca cctctaactg attgttagaa
     4381 gtgtttattt tttcactcaa gtatcctgca cttacaaaaa tatccttttt accgccatcc
     4441 taattctgga gcgatatctt ttacgattgt ttcaagcaag tgcatgttat attctacccc
     4501 caaagtatta ggaatcgtga caagtactgt gtctgcttct ttgattgctt catcttcagc
     4561 tagctccttg attaattgat caggttcacc agcaaaagtt tttccaaaaa tcgttgggtt
     4621 tcggtgataa gcaagatagc cgacagcatc ttgttgcgga ctaccgtctt gtccaaataa
     4681 gcgtcgatcc aaatcagtgg tgattggctg aatcgagcga gtaactaatg ttcttggttc
     4741 aaaatcatga cctgcttgct tccacgcttc tttatacgca cgtaattggt ttgcttgttg
     4801 gatatggaat ggctcgtggg attcgtaatt gactaatgta gatgattgca gattcattcc
     4861 atgttgtgct gcccagattg ccgtttgctg cgaaccagct ccccaccaga ttctttgtcg
     4921 taatccttct gaataaggtt ctatccgtaa ttttcctgat ccttgtggga acattggttg
     4981 aggatttggt tcttcaaatg gttttccttc aagtaattcc aagaattcaa gggtgcgttc
     5041 ccgtgttacc tctttgattt cttcttcact gtaatcataa ccaaagtatt tccagccatc
     5101 tagaacttgt tcaggtgaac cacgccccac tcctagttgc actcttcctt gcgtgatgat
     5161 atctgtcaat ccggcatttt ctgccatata ataagggttt tcatatcgca tatcaataag
     5221 tcctgtaccg atttcaatct tacttgtctt tgcgccgatt gccgctagca aaggaaacgg
     5281 cgacccaatc tgaggggcaa aatgatgtac gcggaaaaac gctccgtcca ctccgatttt
     5341 ttctgcttcc accgctaaat caatcgattg caaataagca tcttgagctg atttcacaag
     5401 cgatccttgc tccatccaat gtccaaagct taaaaagccg atttttttca aggtaatgac
     5461 ctcctttaaa cctacaaaaa tggaaggttt tttattttta atatgaactg attctactac
     5521 tttttttctt aaaagacgaa taattgttct cgcctcacaa tcaagaaatg tatcggctta
     5581 ctacacccct actttcttca tcatctgccg tgtcttaatc tcttttaaca gtctgttaca
     5641 tcgttgattt ttgactcaaa atgattgttt ctaacatgta tttatggtaa aaaagacaag
     5701 attagttctt tgtacacatc gatttatacg ctgaacaaaa agaaatatat aaactaagaa
     5761 acccattgtt ttttcgctcc atcacataga gtttacttta gataaacagt gacatttact
     5821 aatccataac ggaggaaatc aaatgaaaaa aatctatttc ttatgtacgg gcaattcatg
     5881 ccgcagtcaa atagcagaag gctacgcgcg taaatacttg tctccttcaa tgtttgagat
     5941 aagaagtgcg gggatcgaga cacatggctt gaatccaaga gctataaaaa tcatggca
//
LOCUS       DACDRN010000106         5864 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDRN010000106 DACDRN010000000
VERSION     DACDRN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.106"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..3121)
                     /locus_tag="IWJ80_002600"
     CDS             complement(<1..3121)
                     /locus_tag="IWJ80_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP9462278.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDE"
     gene            complement(3135..4352)
                     /locus_tag="IWJ80_002601"
     CDS             complement(3135..4352)
                     /locus_tag="IWJ80_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9462279.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4342..>5864)
                     /locus_tag="IWJ80_002602"
     CDS             complement(4342..>5864)
                     /locus_tag="IWJ80_002602"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9462280.1"
                     /translation="KNYLLGLIFYKYLSDRLLEQVVLLADESLEEYDTVSKQTMLYRE
                     LLSDEESKEDLIATIVDILGYSISPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYN
                     QFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQF
                     ASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHP
                     DNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMN
                     PPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVL
                     FRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASR
                     EFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFE
                     EEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT         1597 a         1245 c          987 g         2035 t
ORIGIN      
        1 actcatcatc taacatttta cgccatgctc gcttcatttc ttgtgggtat ttaaatggtt
       61 tcgcttctgg atgtttggct ttatatcctt cgtaatcctt actgttgata attgctttca
      121 tattaggaat tggatcttca cctggcatat attgcagatt agaagcatgt aattctttct
      181 cagaaacaaa ccactcattg gaaaaggcat gaatcaaatt atcacttgct tgtgtaaaat
      241 agcgacgttt aatgacaaag atatcttcat tactatctaa agtccccaca tcaaatgcat
      301 ttaaaatttg tcggtaaact tctttgactc cttctgtttt attcaattta gccaaagctt
      361 tttcaatttc ttctcgtgca tctgaactat tcgcagcata agttcctagt aattgatcaa
      421 tataagtaga atcaatatca tacaacttag tggtattttc actataaaat tcgatcgttg
      481 aaaaatcttc tccctctttt tctggttctt cctccatcaa tgaacccttc acggtctcgt
      541 acacgccaat ttgttcttcc aaaagttgaa tttgattgtt caaagttgaa gattgcacca
      601 tgtcatcatt gtaatcatca taagtcacta acgattcata cgcacgattc aattcttgaa
      661 atgctttgac gtactcgata cggatttcaa gtggcgtatg ttcatcaatg gattccatgg
      721 atgaaacctc ttttaactta ccatatgctt gcttgaatct ttttttggat tcttggtaac
      781 ttggataaat caaacccgat tcttctttag cttctgaata cagttttgtc gcatcctcga
      841 cattcttctc cattgtcgct ggctttcgga aagtaacaat tagtcctttt gttttttcag
      901 gatacgtacg gttcgtacgt gaaaaggctt ggatcaatcc tgcatactct agattacgat
      961 ccacaaatag ggtttgaatc gtaggggcgt caaaacctgt caatagacga tccactacga
     1021 ttactaaatc gatttgtttc ccaaattgtt tgaattccgc tctttttcgt gccaaacgat
     1081 tattgatgtc tccgttataa cgctcaatgt ctgctagtga ccaggcagta tcataatact
     1141 gattatattc ctcaataatt ttttgcattt cttcttgttg ggcacttgaa tctcggctat
     1201 tttcttctaa tgaataggta atagcgattc gtgggaaatc aggatcctcc atcgttcgtc
     1261 cttcacggat aggctgattg ggaaactctt ttattaacca atcagattct ttcgtcatct
     1321 ctttgatggc ccgataatac tttttggcca tatcaatgga actcgtggtt aagatcgcag
     1381 acttcgttgg acggccattt cgaaaatcaa attttgtata ggcattgtct ggacggaaca
     1441 ttttatgaat gactttttgg atatgttcct ctgcttcaaa aacagtaggg tccaaataac
     1501 tttctttttt gacagactcc atttgatcga tcatacgatt gatctgttcc ggtgaatact
     1561 ctgcatatgt ttctacttct tttaatttcc gatagatttt attctctaat gaagtcggct
     1621 ccacggtgtt ttcatgctct acttgaaatc ctaaaaccga gccatcttct aatgcattct
     1681 tgatggtata ggtgtgcaag acctctccat actgatcatg ggtcgtacgt gccaattgcc
     1741 ctttggcttg tttccgattc tcagggaaaa tcggtgtacc tgtaaagcca aaccaagtgg
     1801 atttcggaaa catttttttg atttcttcca tattttcagc acttaacgct cgatgacact
     1861 catcaactac aaacacgata tgttgcccca ttaatttttg gaagcgattg gttccttttt
     1921 tctcttcctg tttctttgca tatttcaacg ccgcatctag tttttgacgg gttgtaatga
     1981 tcacaacatt cgcattggca tctgccaaca gagtttcact taactctttg gcacttcctg
     2041 ttcccacgat caagctattc gctttggcat taccagaaga aaccccagta ttaaactccg
     2101 aagcaaattt cgtaaactct gtggtggtct gattatctaa atctttgcgg tccactaaca
     2161 tgatcgttcg atcgatcccc gattttcgtg ctaaaagctt agtagataca aaactagtca
     2221 acgtctttcc tgaaccagtc gcatgccaca cataccctga ttgatgttta tttgcggcaa
     2281 taaacaaggc ttgaatcgca tgcacttgat aaggatgtaa caccatgagg gttttattgt
     2341 cttgatcttc actgacaatc gtataatccg caatcaagcg atgggcatct gggatattta
     2401 gcacttgttt gcaaaactca tataagtttt ctaccttttt attatctttc gttcgccaac
     2461 taaataaaaa cttaggatgc aaatccttcg gtaaggcatt tgcaaaatac cgagtcgttt
     2521 gttcattgga aaccacaaac aactgtaagg tcgaaaagat attgttccga aacatgcctt
     2581 cttcagcata ttttttgatt tgattaaacg cctgataaaa accatctttt gcagttactt
     2641 gttttagctc gatttggata atcggtaagc cattgatcaa taaggtgacg tcaaaacgtc
     2701 gatcccgcgc ttctatgtta cttccacgtt ttgcaatttg atggaccact tcataagtag
     2761 agattcctcc accaatgtct tgattggaat aaaggataag tgagacagaa cccaattgcg
     2821 gattttcccg ttcaatcgtg atccgagcca tcccattttc accttttaac catttcgcag
     2881 catcaaatgg ggttttcgtc cgtagcaata attcggtttt gatcgtttca aactctttat
     2941 cggtcaaagg agaatcattc agttctgctt gattatttga gatgattttt tgacgtaaat
     3001 tgctccaaag atcttcttct gattttagat ccggacgata cgtccactga ttatgtcctt
     3061 ccccgaggac acgaatcaac tgttcttcga cttccgcttc ctcacggtga ctaattttca
     3121 ttcttcttcc ctccctaaac aaacatcttt tgcaagaagc cttttttcgt ttccttcagc
     3181 aaatctaact tacgttgatg aagagcgatc gtatcatcta gctgcttgaa gaaggcacca
     3241 attttttgtt gttcatttat atccggtgga aactgaaatg tcctttgttc tataccacct
     3301 ttagtcacat gtaccattgt cgttccgtta gttgattctt taattttctc tttatcagtc
     3361 aatagccaag tatatagata ctgtttgtcc aattccttag ataaaaattt aagtttccaa
     3421 atatgataat ggtagattac acgttcttca ttccacattt caggaccgaa gcttgttgcc
     3481 cataaatata agagatctcc tttattagca tacttgtctt cctctaattc taagtttgaa
     3541 taataccatc tatcattagt attaaaatta cctactctta aaaccctata ttttccagaa
     3601 tcaagtaatt cttgttgttt aaaagctcta ccattcaaaa aaactaccac ttcccccaac
     3661 ttccgctctt cccaatctcc cgtaaatcct ggaaaacgaa cttctggcac ttttgctccg
     3721 tttttaggga acattttttg taagaatcct tttttcgttt ccttcagcaa atctaattcg
     3781 tgctggtgaa gcatgatggt gtgatcaagt tgtttgaaaa atgaaccgat cttgatctgt
     3841 tcgtcataag ttggaagtga gataggtgtc tttttaccac tatcaattgt ataggttccg
     3901 acggttccaa tgcctgcaac gtcacggata tacttctgaa ttttgttatt tatgaacgag
     3961 tagtaaaaga agtttccgtc aattttatgc tcattcttga accaaatgat gtttccatcc
     4021 ttaaagtaaa ttgggttatc atcagtgatt aataagggga cacctattga tccgacacca
     4081 gttacaagca agtctccttg agaaacttta cctgaatttt tcgaatattc attatatttt
     4141 tcttctgaaa tgtaaagata atcggatggt tcctcatttt ttgcagctga gacaatatca
     4201 cgagcacgta aaaatctaat acctgagtta gtccaatctg attgatgaat tcgcttcaca
     4261 gaagtaacat ccatcatatc tcctaactta cgctcttccc aatcttccgt aaaaccagga
     4321 aaccggattt ctggtcgtgt atcattgctc atgtttgaac acctccagcg caccttgtaa
     4381 ccacgcttca ttttcaggtg ttgtttgcag ggaagagata gcttctaaaa gttctttttc
     4441 tagcacttgc tgttcttgtc gtatcttctt gatgtcattt ccgacatgaa ccatatcaat
     4501 tggttcttct tcttcgaaag tatcgacata gcgtgggata tttaagttat agtcattttc
     4561 tttgatttca tcaaaggttg ccaaatgagc gtatttctcg acatcttttc tttcagcgta
     4621 ggtttcgagg attttttgaa tgttttcttc cgataattta ttctgatttt tccctttaac
     4681 aaattcacga ctagcatcaa tgaataatac atctcgagtt tgacgattct tcttcaatac
     4741 aatcaccgtc gttggaatcg atgtaccaaa gaacaagttc gcaggcatac cgatcacagc
     4801 ataaatactg ccgtcttcta ataatttttg acgaatgacc ccttctgcag caccacggaa
     4861 taaaaccccg tgtggcaaga cgatagccat agtccctgtt tccttcaaat gatagaaacc
     4921 atgaagaaga aacgcaaaat ctgctttgga ttttggcgct aatttcccgt agcgattaaa
     4981 ccgagaatca tctaagaagg tcgtatctgc tgaccaatta gccgaataag gtggattcat
     5041 cactactgca tcaaatgtgt aaggttcatc tgttggccag tccttattca acgtatcccc
     5101 attgcggaga ttcatttctt cggcatctac cccatgtaaa atcaagttca tttttgctag
     5161 attgtatgtc gttgtattta gttcttgccc atgatattta acgttatctg gatgagttaa
     5221 gtagttacgt acatttaaca tcaaggaacc tgaacccatg gttgggtcaa agacactaaa
     5281 gaatcgtcgt tctttttgat ccaacgtcac gatttgtgcc atcatatcag aaaccatatg
     5341 aggcgtgtaa aattctccgg ctttcttccc tgcttctgaa gcaaattgac tgatcagaaa
     5401 ttcatacgca tccccgatca catccccatc gtgacctaaa acttccacgt cattcaattt
     5461 cttgataact tcggtaatcg tcacatttcg ttgttgctca tctgttccta gtttttttga
     5521 ttggagatcc acatcatcaa ataacccatt aaattgattg taggtagatg ccaattgcac
     5581 aaaggctttg tttagatcgt tcaattggaa cgttgcttgt tttgcttgat ccgctaagac
     5641 gttgaaaaga tactctggtg agatcgaata ccccaaaata tcaacgattg tggcaatcag
     5701 gtcttctttt gattcttcat ctgaaagtaa ttcacgatac aacattgttt gtttgcttac
     5761 tgtgtcatat tcctctaagg attcatctgc taaaagaacc acttgttcca acaaacggtc
     5821 ggataaatat ttatagaaaa tcagtcccaa taaataattt ttat
//
LOCUS       DACDRN010000107         5748 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDRN010000107 DACDRN010000000
VERSION     DACDRN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.107"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..434
                     /locus_tag="IWJ80_002603"
     CDS             <1..434
                     /locus_tag="IWJ80_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP9462281.1"
                     /translation="KDYWGESKGFIYPDSNMGGNWKATNPKKEQEAMKLKNTKSNNLL
                     YATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSGGQNISSVSYETALL
                     EYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            454..1014
                     /locus_tag="IWJ80_002604"
     CDS             454..1014
                     /locus_tag="IWJ80_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HAP9462282.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGILSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADE
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            1837..2604
                     /locus_tag="IWJ80_002605"
     CDS             1837..2604
                     /locus_tag="IWJ80_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP9462283.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIREKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            2630..3172
                     /locus_tag="IWJ80_002606"
     CDS             2630..3172
                     /locus_tag="IWJ80_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462284.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFATYESGMSAYDETGFDGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGYSLDDKTRLRLFDIEGKQIGLNKFCKNGKEFATKDF
                     IKGYRLLLENYGINLEESVK"
     gene            3173..3739
                     /locus_tag="IWJ80_002607"
     CDS             3173..3739
                     /locus_tag="IWJ80_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462285.1"
                     /translation="MFQKRKDKFPNLEVKSQKVIDGEMIELFQKNYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            3754..4002
                     /locus_tag="IWJ80_002608"
     CDS             3754..4002
                     /locus_tag="IWJ80_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462286.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTENLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            4022..4261
                     /locus_tag="IWJ80_002609"
     CDS             4022..4261
                     /locus_tag="IWJ80_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462287.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            4632..4913
                     /locus_tag="IWJ80_002610"
     CDS             4632..4913
                     /locus_tag="IWJ80_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP9462288.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            4903..5244
                     /locus_tag="IWJ80_002611"
     CDS             4903..5244
                     /locus_tag="IWJ80_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9462289.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVGSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIGEWEKAGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NKFIERLQRKV"
BASE COUNT         2170 a          766 c         1161 g         1651 t
ORIGIN      
        1 aaaaagatta ttggggtgag tcaaaagggt ttatttatcc agattcaaac atgggaggga
       61 actggaaagc tacaaatccc aaaaaagagc aagaagccat gaaattaaag aatacaaaaa
      121 gtaataattt attatatgct acgtgtaaac attttcgaca tgtacgagat acagaattta
      181 cgagttatca tctttcagga atagtaattg acagtttcgt atatgaggct atgggaaatt
      241 ggaaatttgt tgaaaataac agtgggggtc aaaacattag ttctgtatct tatgagacag
      301 ctttattaga atactataac tcacataaag ttatgggcgg tttaaattta tactctcctg
      361 gaagcaatca atttgttaat tctgatagta gtatcatatg tttagaaaaa gtattaaaaa
      421 aaatagcttt ataaagtttt atggaggaac aacatgggag acgaaaaaaa gcgcttggaa
      481 gttcaattac gtgaagcata tggacgagta acgtatactt acaccactca tttaaagcaa
      541 gccgataata ttatttctga caataaaatt ttaaggtgtc ttcaaattgg tttgtcagcc
      601 cttactactg gagggatact tagtaccatt attactgata atagtgagtt aacctttgtt
      661 gctggattat gttcaacaat ttctcttgga ttaaacttat actttaagaa ctttgatctt
      721 gctgaagaag ctaaaaatca tcaaattgtt gccgatgaat tatggttgat cagagaagaa
      781 tacgtaagct tactaacaga tttaaatcgt ttaagtgttg aagatattgt gaaaaaaaga
      841 gatcaattaa tgaacgaaac tcatgaagca tataaaaatg cccctaaaac aaatagtaaa
      901 agctatgaat cagctcagaa agcattaaaa tctgaagaag agcaattttt tacagaagag
      961 gaattggata agatgttacc agagcatctt agaaaaaaga gttctaaata ttgaactctt
     1021 tttttatgct taaaattctc ttgtaatttt tctattcaat caataaataa tctatattta
     1081 agaagaaata tgatgataga aacatctcct aaagccaccg caagccgttc taagcatagc
     1141 ttagcccgac attgctagat atgctcatct caccatgaac gtttcctaaa gccaccgcaa
     1201 gccgtcctag tcattgccta gtccgacatc gctagattag tgcatctcgc cataaaagtt
     1261 ttctaaagcc accgcaaacc gtcctagcca tcgcctagtc cgattttgct tgatcggttc
     1321 catttctgtt tatttagtgg gtcaacaccg taatcttaaa tagccatcgc ctatttaaga
     1381 ttactaggtc tgctttgaag gtaaaaagtc tttcattctt caagactttt aggctttcaa
     1441 gctacgcctg aaagcattga aattcatcgc tctttatttg gttttgccat ttataattcc
     1501 ttacgcccat tatctttttc gcctgtcttc cttgtcagaa gtgtttagag cttatctcat
     1561 ctttggatta atacaagtgg cgtaaatttt tttctaaacg caagcgttac gaaaataatt
     1621 tacttaaaca cttgtattaa tccggcagat aagaacgcat tctaatcact cccgacaagg
     1681 caagacaggc gaaaaagata aataaatgaa cgaaaggaag aaacataaaa tgcaaaacca
     1741 aatagctatg aagaaaaaaa cacctccctc taaaacaatc caaacaaaaa agaattttga
     1801 aaaaccaaaa atcaaaaaca gaaagaagcg ttcaacatga gagatagaat tttagaagtt
     1861 aatcaagaaa tggtaaacaa gttaacaaat atcagtggga actatgaaaa gtacgatggt
     1921 actttacttg ataactatat ttttcacgat acacaaaata tcagaattgg cagaagtaaa
     1981 ccgagagcgt tcattctcat cagagaaaaa tatttgaatg aatggaatag cacctatgaa
     2041 atggttatga ctgataactt tgaaaaggta ttggatttca gaaatgcttt gttgggttct
     2101 gatatgggaa atagcagtct tgaatctatt gaagttgaaa acaagaccga gaatgaaggt
     2161 gaaggattga ttaagaatgt cttgaaaatc gaacaaaaat atatcagtga agatagaagt
     2221 ttttatagag tcacaagctc ggttggtcta gcaaactttg ctactaactt aattggaaac
     2281 aatgctagtg aatctttgct agtgatgtgt ctgaatacaa agaatgaggt cattgcttat
     2341 agcgaagttt tcaaaggtgg aattaatcaa tcagtagcga taccaagaga gatttttcaa
     2401 ctagctttgc taaacaattc agcaaaaatc gcagtatcac ataatcatcc gagcggatat
     2461 gaatttccaa gtgaaaatga caaaagtttt actacgaatt tgaaaaacgc aggaaaaaca
     2521 attggtattg aattgttaga ccatatcatc gtaacaagta aaaacacaag ctattactca
     2581 tttcaagaaa atggttcttt ataaaaatta gagaaaagag gaaaacaaga tgggcgaaag
     2641 aacacaagcg ttaattacag tcaaatatgg taacagcaaa acgaaagcaa gcgtgtcgat
     2701 tcattatcag tgggggtatg gtcgatatat gctgatggac gtattgaatc aaggaattta
     2761 tttaatcaat aaagcatacg acttacgaga taaatcaaga gaagaattag caaaaggaat
     2821 ctttgataga agtacagggg ttatcaattt tgccacctat gaatcaggta tgagtgctta
     2881 tgatgaaaca ggatttgatg ggcgctatga accttatcaa gaaacgattg aagatgtgat
     2941 gaagttttct gataacaatg atggctatgc tcatttagag atggtgttcg atgaaaatgg
     3001 atatagtcta gatgataaaa ccagattgcg attgtttgat attgagggta aacagatagg
     3061 actaaacaag ttttgtaaga atggaaaaga gtttgctaca aaagatttta ttaagggata
     3121 cagattgttg ctagaaaatt atggaataaa cctagaagag agcgtgaaat aaatgtttca
     3181 aaaaagaaaa gacaaattcc ctaatttaga agtgaaaagt caaaaagtga ttgatggtga
     3241 aatgattgaa ctattccaaa aaaattatgg aacgatagaa actaccgcaa gttgcaaata
     3301 ctactttcaa ggaaagagaa acacgtttaa aggctttgcc gattctgatg aagaattggc
     3361 aaagaaaatc attgattggc ttgtcagcag tgaacacgta gcaagagaag ataccgttag
     3421 aacaacgatt aatggggaag tcctaacagg tactagatgt gtttgttgta atcatgaatt
     3481 tgtagaagat gaaggttgct accgaatcaa tgaatatggt gattttattt gccagagttg
     3541 ccacgagata gaatttccaa ccgaaagaag ttggtttgaa tacatgctac aagattatga
     3601 gatggaaaaa gaaaatggtg aagtagtttt ttgtaccaaa gaggaattga gtaagagaag
     3661 cgatgatgaa attgaagatt ggtgtgtgtg gttagtaggg gcagatgatt gcgttgcata
     3721 ttacacaaca gcagaatagg agtgtgaatc agcatggaag gaatggattt tctagatcat
     3781 gaagatttgg ttgattttgg ttatacatgg aaaggaatgg taggtattag cagaagtctt
     3841 gctaatgcct tttacgaaag aaattatgca gtatatgttc tttatgatga cgatacagaa
     3901 aaccttgttg acgaggaata taaattggat ttagagaatg ttttatatgg aatagaaaag
     3961 gaagatttag caaaatatat tttttcatgg ttgggtcaat aagtgaaagg aagatagaaa
     4021 catgacaaaa tttaaaagta agggtatgca atggtgttct tattattatg ttggaaatga
     4081 tgaagattta ttgagaaaaa cccaacgaaa aggttatcca gataagatat tgaaacaaat
     4141 aggttattac atgccagaag agaggataga aaaagaattt tgtgaggttc tttttgatag
     4201 tgaacagcta ttagttttgt taacccaaaa taaattgatt tgccaatttg aaaataatta
     4261 agatgaggtg gtgaaccatg aatgaagata agatggtatt aagtgaatat gctagattga
     4321 gaactattaa ttctttagca tctcatttaa gttgttcttt ccttacaaag gtgctaaaat
     4381 taatttacaa atgtgaagat tttgtacaag agaagcacaa agacaagcct actgcgaata
     4441 ggcttgtctt tttttcattc attacgattc cgtatgtgtg ggttagcgac aatttgttca
     4501 attaccgtat gatatagcat cttaatttga ttagattact tgcaatgtag tttattaaga
     4561 gtataatata atcataaatg tggataattt gagaaataat gagtgtttta tactcaaaag
     4621 gagtggatga aatgaaggtc aaaaatccaa ctgaaaaaag cagaatccaa gtcggaatag
     4681 acaagaattt aaaagagaac gcagaaatga tattggaaga gctaggttta aatcctacaa
     4741 cagcaataac aattctttac aaacaagtag tcgctagagg tgagtttcca gtagagataa
     4801 agctaagtga agaggagaag caaggaatca gattgcaaca gctaactaag gacatgccag
     4861 tagatgttct agatacagat gaaaagttag aagagtggtt cgatgaagcc taatgagata
     4921 gtaacggtct atgttgcatt tacagataaa aatggtggta aaagaagacc tattttggtg
     4981 ggtagtgata aagaagatag agtagaattt tttggaatca caagtcaata tgagaagaaa
     5041 tcagaccaaa taaagagagt gtattttcct attggagaat gggaaaaagc aggtcttaaa
     5101 aagcaatcat ggattgacgt agggtcttta aaggctattc ccaaaagccg agagaacata
     5161 tcatttaaaa aggttggtat gctatcagct agtgatacga aaaagctaaa taagtttatc
     5221 gagagattac aaagaaaagt ctaacaaggc ttttcttttt ttgcttatat gtatattaag
     5281 atatacgtct atacaaacgt atttatgtat ataaaaatac acgtaaatat atacgtgaat
     5341 ataaacatta agaaaatatt aagaatgatt aaatagtggt aattgttaat atacgaatta
     5401 taagatatat tttaaatcga atgtatgtaa ataaataaaa tgactggagg atatttaata
     5461 gtgataaata acagtaatcc agacggatac agtgagtgaa gtagcttgct acgaacgaac
     5521 aagcagaatg aaacggtagt ttcattagag caagtcgctt gcgacgtagc gttaccgctg
     5581 atgtaactgg caagttacat agagagagtt tgcttgcaaa cgaacgttac ctacttgaat
     5641 agaaaacaaa aataacagat tagtgaattt taaataggag ccacaactca accaaccgct
     5701 tgcggtgggt gagtcccacg gaggaaaaga cgaacgaacg gtcgcgta
//
LOCUS       DACDRN010000108         5709 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDRN010000108 DACDRN010000000
VERSION     DACDRN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.108"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            115..1014
                     /gene="rsgA"
                     /locus_tag="IWJ80_002612"
     CDS             115..1014
                     /gene="rsgA"
                     /locus_tag="IWJ80_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP9462290.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            1027..1677
                     /locus_tag="IWJ80_002613"
     CDS             1027..1677
                     /locus_tag="IWJ80_002613"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP9462291.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            1678..2316
                     /locus_tag="IWJ80_002614"
     CDS             1678..2316
                     /locus_tag="IWJ80_002614"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP9462292.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            2471..3325
                     /gene="mreC"
                     /locus_tag="IWJ80_002615"
     CDS             2471..3325
                     /gene="mreC"
                     /locus_tag="IWJ80_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP9462293.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVASVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            3329..3838
                     /gene="mreD"
                     /locus_tag="IWJ80_002616"
     CDS             3329..3838
                     /gene="mreD"
                     /locus_tag="IWJ80_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP9462294.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            4093..5667
                     /locus_tag="IWJ80_002617"
     CDS             4093..5667
                     /locus_tag="IWJ80_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462295.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         1921 a         1093 c         1185 g         1510 t
ORIGIN      
        1 ctcttttttg tgtttctatc tcttttgatc aattttcaag ccatatactt tgagaagttt
       61 gcatggaata acacctactt tcctcttcgt atggtaaaat aaacaaaaac ggaggtggca
      121 cttatcaaag gacaaatcag aaaagcaatc agcggttttt actatgtata cgcaaacgga
      181 gagacctatc aaacaagagg aagaggaaat ttccggaatc ggaaaatcac gccgcttgtc
      241 ggagatcagg tgatctttga gagtgaaaat caaacagacg gctatcttct ggaaatcttg
      301 ccgagaaaaa atcaattagt tcgcccacca gtcacaaatg ttgatcttgg aatcattgtc
      361 acaagtatcg tagagcctgc attttcttat aatctacttg accgctttct agttaccttg
      421 gaatacgagt cgatcgaacc gattattttt ttgacaaaga ccgacttgtc agacgaccca
      481 gaaaaaatcg aagagatcaa gcaaacctat actaaaatag gctatccggt gattgtaccc
      541 aatcatacgg gtgatacgac agaactgatc cgttacttcc cagaacggct atcggtcttt
      601 atgggtcagt ctggagcagg aaaatcgacg ctactgaatc aaatatcacc ggaactgaat
      661 ttagcaacag gagagatttc tgatgcactt ggaagaggac ggcatacaac acgtcatgtg
      721 gaactgattc ctatctatgg cgggttagta gcagatacac caggatttag ctcgattgac
      781 tttttgacaa ttgaagcagg agaactgccg agacaatttc ctgaatttgt agaagcttct
      841 gtccattgca aatttagaga gtgcatgcat gctaaagaac ctggctgcga agtaaaacat
      901 agagtagaaa caggtggtat cgcacagaca cgttacgaca attatttgca attcttacaa
      961 gaaatcgaaa aaagaaaacc aatgtatgca aaaaaagaag gtaaacgaaa ataggaggaa
     1021 acaagaatga agatagcacc atccattttg agtgcagatt ttgccaattt acaaagagac
     1081 attgagttag tggaaaaagg aggcgcagat tacatccacg tagatgtaat ggatggccaa
     1141 tttgtcccaa atatcacatt agggccaaat attgttcagg caatccgtcc tgttactaaa
     1201 ttaccgttag acgtccatct gatgatcgtt gatccagaaa actacattcc agcatttgca
     1261 aaagctggag cagatatcat tactgtccac gtcgaagcaa ctcctcatat tcatcgggca
     1321 cttcaaatga caaaaagctt aggagttaaa gctggagtag ttatcaatcc aggaacacca
     1381 gtctccatga tcaaacatgt tttgcctttg gctgatcaag tactagtcat gactgtcaat
     1441 cctgggttcg gcggacaaag ctttattgaa gaaacagtag aaaaaattac ggaactcgct
     1501 gatctacgta gagagaacaa ttggacttat gcaatcgaag tagatggcgg aatcgttcca
     1561 gaaacagcaa aaatctgtaa agatgcaggg gcggatgtct tcgttgcagg atcatatatc
     1621 tatggagccg aaaatccagt cggacaaatc gaacgattaa aagaggcatt agactaaatg
     1681 aaaattttat tagctgctgg aggtaatccg caaaactggc cgcagtctct gctgtccaaa
     1741 tacgatgcct atgtcgggat agaccgtggt gcgctgtacc tgcttcaagc aggctaccca
     1801 gtagatctgg cagtgggtga ttttgattca ttgtcgaacg aagagcagaa ggaagtattt
     1861 gcaaaagcca aagagtatac ccgttcccct gcagaaaaag acgatacaga tacacagtta
     1921 ggtatcttaa aaacatttga acgttacccg caagcggaag tcactttgat tggagctacg
     1981 ggcggaagat tggatcatct tttagccaat ctctggttag gtatggaacc tcgcttcaaa
     2041 ccctttgccc atcagcttat tatcagagat tgtcaaaatt atttgtctta ttatttgcct
     2101 ggagagcata tagtaagaca gataccagat atgaagtatt tagcttattg ctgcttgact
     2161 cccgtctcga atcttacatt aaaaggcagc aagtatgtat tggaaaataa agaagtagaa
     2221 tatccgacat cttatgcaag taacgaattt ctttcagata ccgcagaatt ttctttttca
     2281 gatggtatga ttgcagttat tcaaagcaaa gattgatcca taaacaaata aaaaaatttg
     2341 tctttcaggc taaaaatgac agaatctatt atttgctaaa aaaaagctta agaggagcaa
     2401 cttacctttt gtttcacggc atttagtgat aaaattgaac acaagagtct aattaattcg
     2461 aggtgtcttt gtgaagaagt tcaatcctaa taaaaatatt attattacct tggttattgt
     2521 tatcatcgtg gtgacgatac taagtgtgac cgctgcgcag cgagcgaatg aaggaaagcc
     2581 caatttcttc caatccatcg ttaatgacag tgttgcaatg gttgataaaa caatctctgc
     2641 cccagtacgg tgggtacgaa atggagtagc ttctgtccat aatctttttt caacttatgc
     2701 cgaaaatgag cggctaaaag aaaaaatcga tagctatgat gacgtttcac agcaaaacaa
     2761 gaattacgag agagaaatcg aagcgttgaa aaaagaactt gagttgaatc agacgctgac
     2821 tagttacgaa aaagtaacgg caaatgtcat tactcgttcg cctgacagtt ggcaggacat
     2881 gctgatcgta gacaagggtt ctaaagacgg tatcgaagca aatatggcag tcatgtcgca
     2941 aaaaggattg attggccgag tgatcgaggt caatacggct tcgtctaaaa tcgaattact
     3001 gtcatcctct aatgaaagct ccaatcattt tccagtacgg gtatcttcgg ctaatggcga
     3061 agcgtttggt ttgcttaaaa actatgatga aaagctccat gccttagtgg tgacccaatt
     3121 aactggtgat acggatatca aagaagggga tgttgtccag acatccggtc ttggagggaa
     3181 ttctccagct aacttgccga tcggtacggt tattaaaacg aaaccagata gttatgggct
     3241 ggatcgggaa gtttatgtga aaccttatgc agaaatgtat gacgtgtcag ttgtgacgat
     3301 tgtccaacgg ctagcggagg attagtccat gttgaagaaa gaaacaatga agtactatct
     3361 gccaatcgtt ttgttctttt tgatgttgat agatggtcat ttaacaagaa tgctagggga
     3421 gtggtcgaaa ggcacctata tgtcaaatgc ccactttctg atattggcat tattatgttg
     3481 cagtatggcg tttgaaaaac gttatttact gattaccacg attgttctcg gggctatcta
     3541 tgacgcttac tatattggcg ttatcggtat ctatgcagta gctctccctt taattgtatg
     3601 gttgatgtat gtaatgaaag acgttatcca tgtcaacatc tttactgaat ttttcagtat
     3661 gatcatcttt gtcacgggtt atgaattgtt tacgatggtg gtccagttga tttttaaatt
     3721 agcagtagta aataacacgt attttattac aaggttttta ggacctacac tgctgttgaa
     3781 catgattata tttgtattat tcatttttcc ctttaagaaa ttattcagtg atgaataatt
     3841 tcttttttta tttttaggtc gaaagcgaaa aagaatcgtt gttatcttaa gaaatgctag
     3901 acttaacgcg gctttttcat gatcattttc tgaaacgaac agttttgtca tttttaaaag
     3961 ttgttttgaa acatttttgt aacataacta ttatatcgtt gacacactgc tatggtacaa
     4021 tttttatatc gttaagaaag acttaaatat atttagatat ttattaaatt aagccagtcg
     4081 gaggaatgaa gagtgaaaaa gagtttaata tcagcagtaa tggtatgttc aatgaccttg
     4141 actgccgtag catcaccgat cgctgccgcg gcggacgatt ttgattctca gatacaacag
     4201 caagatcaaa agattgcaga cttgaaaaat caacaagcag atgcacaatc acaaatcgat
     4261 gccttagaaa gccaagtttc tgaaatcaac acacaagcac aagacctatt agcaaaacaa
     4321 gatacattgc gtcaagaatc tgcacaatta gtaaaagaca ttgcagattt acaagaacgt
     4381 attgaaaaac gtgaagatac aatccaaaaa caagcacgcg aagcacaagt aagcaacaca
     4441 agctcaaact acatcgatgc tgtattgaat gctgattcat tagcagatgc tattggacgc
     4501 gttcaagcaa tgacaacgat ggtaaaagca aacaacgact tgatggaaca acaaaaacaa
     4561 gataaaaaag ctgtagaaga caaaaaagct gaaaacgatg ctaaactaaa agaattagct
     4621 gaaaaccaag ctgctttgga atctcaaaaa ggcgatcttt tatctaaaca agctgatttg
     4681 aacgtactga aaacttcatt agctgctgaa caagcaactg ctgaagacaa gaaagctgac
     4741 ttgaaccgtc aaaaagcaga agctgaagca gagcaagctc gtattcgtga acaacaacgt
     4801 ctagcagaac aagctcgtca acaagctgca caagaaaaag ctgaaaaaga agcacgcgaa
     4861 caagcagagg ctgaagcaca agctactcaa gcttcatcta ctgcacaaag ctcagcaaca
     4921 gaagaatcat cagctacaca atcttcaatg actgaagaat catcatcagc tacacaatct
     4981 tcagcaacag aagaatcaac aacacctgaa tcttcaacag aagaatcaac agcgcctgaa
     5041 tcttcagcaa cagaagaatc aacaacagcg cctgaatctt cagcaacaga agaatcaaca
     5101 acagtgcctg aatcttcagc aacagaagaa tcaacaacag tgcctgaatc ttcaacaaca
     5161 gaagaatcaa caacacctgc gcctacaaca ccatcaacag atcaaagtgt tgatacagga
     5221 aacggcacag gaagtagtac tccggctcca acgccaacac caacacctga acaaccaaaa
     5281 cctgtaacac ctgctccagc tccttctgga agtgtgaatg gtgcagcaat cgtagcagaa
     5341 gcttacaaat atattggtac tccatatgtt tggggcggta aagatccaag tggctttgac
     5401 tgctcagggt tcacacgcta tgtttacatg caagtaactg gtcgtgacat cggtggttgg
     5461 acagttcctc aagaatcagc tggaacaaaa atttcagttt ctcaagcaaa agctggagac
     5521 ttgttattct gggggtcaca aggcggaact taccacgtag cgattgcttt aggcggagga
     5581 caatatatcc atgctcctca accaggcgaa agcgtaaaag ttggttcagt tcaatggttt
     5641 gcccctgact ttgctgtcag catgtaagaa tgattaatca aaaagagtta gcccattctg
     5701 gactaactc
//
LOCUS       DACDRN010000109         5697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDRN010000109 DACDRN010000000
VERSION     DACDRN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.109"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="IWJ80_002618"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="IWJ80_002618"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="IWJ80_002619"
     tRNA            203..276
                     /locus_tag="IWJ80_002619"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="IWJ80_002620"
     tRNA            316..405
                     /locus_tag="IWJ80_002620"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="IWJ80_002621"
     tRNA            420..491
                     /locus_tag="IWJ80_002621"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="IWJ80_002622"
     tRNA            507..579
                     /locus_tag="IWJ80_002622"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="IWJ80_002623"
     tRNA            589..661
                     /locus_tag="IWJ80_002623"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="IWJ80_002624"
     tRNA            697..772
                     /locus_tag="IWJ80_002624"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="IWJ80_002625"
     tRNA            774..854
                     /locus_tag="IWJ80_002625"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
     gene            861..934
                     /locus_tag="IWJ80_002626"
     tRNA            861..934
                     /locus_tag="IWJ80_002626"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Trp,seq:cca)
     gene            937..1009
                     /locus_tag="IWJ80_002627"
     tRNA            937..1009
                     /locus_tag="IWJ80_002627"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:970..972,aa:His,seq:gtg)
     gene            1021..1092
                     /locus_tag="IWJ80_002628"
     tRNA            1021..1092
                     /locus_tag="IWJ80_002628"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1053..1055,aa:Gln,seq:ttg)
     gene            1138..1208
                     /locus_tag="IWJ80_002629"
     tRNA            1138..1208
                     /locus_tag="IWJ80_002629"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1170..1172,aa:Cys,seq:gca)
     gene            1229..1313
                     /locus_tag="IWJ80_002630"
     tRNA            1229..1313
                     /locus_tag="IWJ80_002630"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Leu,seq:caa)
     gene            1375..1447
                     /locus_tag="IWJ80_002631"
     tRNA            1375..1447
                     /locus_tag="IWJ80_002631"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1408..1410,aa:His,seq:gtg)
     gene            1457..1528
                     /locus_tag="IWJ80_002632"
     tRNA            1457..1528
                     /locus_tag="IWJ80_002632"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1489..1491,aa:Gln,seq:ttg)
     gene            1546..1630
                     /locus_tag="IWJ80_002633"
     tRNA            1546..1630
                     /locus_tag="IWJ80_002633"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1580..1582,aa:Leu,seq:caa)
     gene            1885..2760
                     /locus_tag="IWJ80_002634"
     CDS             1885..2760
                     /locus_tag="IWJ80_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9462296.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            2864..3628
                     /locus_tag="IWJ80_002635"
     CDS             2864..3628
                     /locus_tag="IWJ80_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP9462297.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            3645..5057
                     /locus_tag="IWJ80_002636"
     CDS             3645..5057
                     /locus_tag="IWJ80_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9462298.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            5074..>5697
                     /locus_tag="IWJ80_002637"
     CDS             5074..>5697
                     /locus_tag="IWJ80_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP9462299.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIY"
BASE COUNT         1719 a          965 c         1286 g         1727 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg
      961 caccggattg tggctccggc attcgtgggt tcgattccca tcattcgccc tttttattct
     1021 tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc
     1081 cagctacccc agtttctata atttattctt tattttatag attgataata gtataatggc
     1141 ggtatagcca agtggtaagg cagaggtctg caaaaccttc atcaccggtt caaatccggt
     1201 taccgcctta gtactaataa atcaacttgc cggcgtggcg gaattggcag acgcgctgga
     1261 ctcaaaatcc agtgcccgtt gagggcgtgc cggttcgacc ccggccgccg gtattgacta
     1321 agatgtcttc atcttagttt tttattgaga aattaaataa catcttttat gatggcgaat
     1381 gtggcgaagt ggttaacgca ccggattgtg gctccggcat tcgtgggttc gattcccatc
     1441 attcgccctt tattcttggg gtatagccaa gcggtaaggc aagggacttt gactccctca
     1501 tgcgttggtt cgaatccagc taccccagtt attattttta aatttgccgg cgtggcggaa
     1561 ttggcagacg cgctggactc aaaatccagt gtccgttgag gacgtgccgg ttcgaccccg
     1621 gccgccggta taattatcaa tatagcaact ttcttataga gagttgctat attttttttg
     1681 tttttatgta attacctcca aatcaattcg atacaagtat tatcataaaa cgagtaaaaa
     1741 agatattgat aacgctttta ttatgtgtta aactattttt gtaaattatg tagggtgcta
     1801 ctattcaatt aggcacgacc taagaaaggg ttctttctta ggtcgttttt tgtctttaaa
     1861 aattgtttgg agacaaaaat atgtatgaag gagtctgcaa gttgttttgt tttatcatat
     1921 agaggaggtg aattaacggt gtatgtgatg aaaaagctga atcataatgc tgtattgatc
     1981 gatgacgatg gaatagaaaa aattgcgatg gggaaaggga ttggcttttc tgctaaagtg
     2041 agtaagccat ttgatgttga ggatgctgac aaattatttg tattagattc taaagaaaag
     2101 atcaaacgat tcagtgagat agtctcgcag attccaatcg aatttatgga ttttgcggaa
     2161 gaagtgattg aactgataac agctaggatc tcctctccat tagattcaaa tatctatatt
     2221 acattgacag atcatattta ttttgctatt caacgaaaaa gagaagatag agatgtcaca
     2281 gcaattatgt tgccagaaat gagattcctt tatccaaatg aatatgatat agcagtagaa
     2341 gtggtcgagc gaatcaatca tcgatatcaa accgagttag ggtcaaatga agtaggattc
     2401 atcactatgc atatcattaa tgctgagcta ggagagccta atagtttaaa gagcttaaaa
     2461 atagtagaaa tgacgaccac gattttgtcc catctagaaa aatcctattc aaacgtttat
     2521 gataaagaat cactgactta tcatcgctta atggttcata tcaaattcct ggttaaacgc
     2581 ttgatttatc aggaagaaac gccagtagaa aatattggtt tttttactga aacatttaaa
     2641 catagcaaag cgtattgtac agcactaaag attaaagata tcatcgaaca gcaatttcaa
     2701 cttgccattc aagaaaatga aatcatctat ttggcaattc atctagcaag aatctattaa
     2761 aataagggcg taactatcta taggcgcaac caaatgacac atagtaaatg gactatgatt
     2821 atttggttgc gcctatttta gtagaaaaaa gaaggagaat gaaatgggaa aagtgatttt
     2881 taattcggat gattttggct atagccatgg tgtgaattat gggattatgg atgcttatca
     2941 gcgaggaatt ttgacatcaa ctacattgat ggcaaatatg ccgggtttcg agcatgctgt
     3001 gaaactaaga aaggaaatgc ctaggctagg tgtaggagtc catttaacat tgacgtgtgg
     3061 caaaccactc ctcaaaactg tcgatactct tatcgaaagc ggagagttca gatccttatc
     3121 ttattatcag catccattta acattgacaa agatcaactg tatcaggaat ggaatgctca
     3181 gattcaaaaa atttatcatg cggggatcat accaactcat ttagacagtc atcatcatac
     3241 tcatactttg gatgaaaatc aggaggtagt tgttacatta gcaaaaaaat atgatttgcc
     3301 ggttcggagc aactttgaac gaaaagatga agttcgtcat gtcgattatt ttgagccttg
     3361 tttcgatgtc gtaggagaaa ctgatgaaaa aaaacgacaa atcaatacga gtgtggaaga
     3421 atatttggca agtttactca tgatattgag agaagataaa acgatcgaag tcatgtgtca
     3481 cgcagcgtat ttggatcagg aattgttaaa tggttcaagt tttgtgtatc ctcgtaccca
     3541 ccaagtcgaa tttttaattc attcagcatt tgctcaaaaa gtgaaaaatg atcctttgat
     3601 tgagttagta acatatgagg atatttaata gaggaggaat aaaaatgaat gttaaatcaa
     3661 atgtgcagaa gcttggaaga gcgatgctac ttccagttgc agcgatgcca ttagcgggat
     3721 tgatcatgcg tttatcagct gacgatatgc tgaacatccc agtaattggt gcagctggaa
     3781 atgccgtttt tggaaacttg gatatcttat ttgctatagg agttgcaatc ggttttgtca
     3841 aaaataagga caaaggaata cctgcactta ctggtttttt agcgattgct accttgaaaa
     3901 aaggcttaga aattatggac ccagaagtga atatggggat ttttggtgga attatttccg
     3961 gtttagtagc ggcatggacg tataatcgct ttaaagaaca aaaattacca gtagtttttt
     4021 cttattttgc gggcgaaaaa ttcccattga ctatggtgat gattctacaa acaatcgcag
     4081 ctgttttttt gggctttatc tggccgctca tccaaattgg gattgataat tttgcaaaac
     4141 tgctcgttga tatgggagca ttaggtgtgg ggatatttat gtttttgaat cgtttgctga
     4201 tcccctttgg actgcaccat gtgttgaata cgtatattta ttacgattta ggaagctata
     4261 catctcctag caaggaagtt ttcagaggag agatgacacg ctttatcaac ggagatcctc
     4321 aggctggact gtttttatca ggtttttttg tagtaatgat gtttggtata ccagctatat
     4381 gtctagctat ctataaagca gcttataaag aaaataaaga gatggtcaaa ggaatcatgg
     4441 gaagcggtgc aggaacttca tttattgcaa atatcactga gcctgttgaa tttagtttca
     4501 tgtttctttc tcctatgttg tacgtgatcc atgcactttt tgccggttta gcagggattg
     4561 tctgttactt attcaatata cgaattggtt ttacattcgg agcatgtatt gttgattatc
     4621 tcatcaattt tcgaatcgct accaatgcta ttttgattct tccaatcgga attttctttt
     4681 ttgccttgta ttacgttact ttttattatt tgatcaataa gagaaacatt cagacattag
     4741 gacgtgaagc taaggcagag tttggcaatg aagtaacctt agaagaaaca gagttagggt
     4801 tagcaagtaa aaattatgaa tatatggcaa caaaaatgct acaagcgttt ggaggaaaag
     4861 ccaatatcct ggatgtctat agttgtaata cacgcctacg ggtagaggta gtggatccca
     4921 ccatggtcga ggaacagcga atcaagcaat tagggataag cgggatcatt aaaccaactg
     4981 aaaaaaatta tcaaattatc atcggcttag aagtgactta tgttatggca gaattcaata
     5041 aattattaga agaatagaaa ggaaaagatg atgatgaaaa aacagattat cactatagct
     5101 ggaggcggga gtacgtatac accaggaatc gttcaagcca ttttgaacaa tgaaaaacgg
     5161 ttgccattat ctgaaatacg tttatatgat attgatgaag aaagaaatat ggatatgtac
     5221 ttgattgtta aatttatgct gaaaagaaaa ggtttttcaa atattcgcat tcgtgcaacg
     5281 gatgatccaa agcttgcttt tacaggatgc gattttgttt tctcacaaat tcgtgttgga
     5341 ggattggaga tgcgagaaaa ggacgaaaag attccactta aacatggatt agtaggacaa
     5401 gaaacgtgtg gactcggagg atttgcttat gggatgcgct caatcaaagg tttattggaa
     5461 attgtcgatc atatacaaga ctatgccccc aaagcatgga ttttgaatta tacaaatcca
     5521 gagtcgatcg tttctgaagc tgttcgtcgt aaatatccga atgtaaaaat gattaatgcc
     5581 tgtgatatga ctatttcaat tgaagaaacg attgcagtga attacggtta tgatcgtaag
     5641 aattggatcg ttacgtatta tggtttaaac cactttggct ggtatacatc gatttat
//
LOCUS       DACDRN010000110         5582 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDRN010000110 DACDRN010000000
VERSION     DACDRN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.110"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..701
                     /locus_tag="IWJ80_002638"
     CDS             <1..701
                     /locus_tag="IWJ80_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP9462300.1"
                     /translation="KELQREIMPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITK
                     NFPSNIPNNYLEYYLYPDMVVEHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVL
                     NFNFGEHGQYIVDIAISLLNDDRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEP
                     ITLRKPISDFHKGLMEAQVAVEKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILD
                     EMIKVNKKYWPALK"
     gene            714..1172
                     /locus_tag="IWJ80_002639"
     CDS             714..1172
                     /locus_tag="IWJ80_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9462301.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            1464..2765
                     /locus_tag="IWJ80_002640"
     CDS             1464..2765
                     /locus_tag="IWJ80_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9462302.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            2792..3052
                     /locus_tag="IWJ80_002641"
     CDS             2792..3052
                     /locus_tag="IWJ80_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9462303.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVTHLNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(4051..5286)
                     /locus_tag="IWJ80_002642"
     CDS             complement(4051..5286)
                     /locus_tag="IWJ80_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP9462304.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1724 a          858 c         1144 g         1856 t
ORIGIN      
        1 ataaagaatt gcaacgagaa attatgccag agattattga aaaactaata acaaaagaaa
       61 tgcaagtcgc cgattttaat ataggagata aaacttggca aaaaaccttt caaatgatgt
      121 cagtcatcac gaaaaatttt ccaagtaata tcccgaataa ttacttagag tattacttat
      181 atcctgatat ggtcgttgag catactgaca aagagtatac acgtgctaat atggtcatgg
      241 atggacgaga gaaaaatacc aaagaaatgg ctgataaaat acgccgcggt attgtggaag
      301 aagtattgaa ctttaatttt ggggagcatg gacaatatat cgtcgatata gctatttcct
      361 tgttgaatga tgatcgtcgt cgctttatgc taatcgttcc taatcaaggg gcgattccga
      421 atctacggtc agatgcagtt gttgaaatac cggcatatgt tggtgcaact ggtgtggagc
      481 caattacact aagaaaacca atcagtgatt ttcataaagg attgatggag gcacaagttg
      541 cagtagagaa gttactagta gatgcttact ttgaaggttc ttatcaaaaa gctttacaag
      601 catttacatt gaatcaaacc gtaccaaatg ccagagtcgc aaaaaaaata ctagatgaaa
      661 tgatcaaagt aaacaaaaaa tattggccag cattaaagta gaggtgataa aaagtgcatt
      721 tctttaaaac aagaaacatt ctaaaagctg tagctagtgg tcaaattatt ccattaacag
      781 cagtaaaaga cgaggtcttt tcttcaaaaa tgataggagt tggatacgct ataacgaatc
      841 atgatggata tatccatgcg ccagttaaag gaataatcga taatatattt cctacccgac
      901 atgccataac gatcgaaagt gaatctggca gaaaaatact gatccatatg ggtttagata
      961 cagttgaact cgaaggaagt ccgtttcaga tacgggttga aagtaaccag gaggtaaaag
     1021 aagggacact gttagctatt atagatttga agaagttgga gcaagcacaa aaagatacgg
     1081 tgatcattgt agtttctcca ggcatttttg aaggaaattt attaaaacaa aaccaagaag
     1141 taaagattaa agatagttgt tttgaatttt agtgtacgat gaaaaaagtt aaatagggtg
     1201 atatttatgg gtgaatttac aaaatttttt tgaaatgata tgtttcaatc actagattta
     1261 atcattttga tttttgttaa ttatcgaatt tttttgttgc tcctatgatt tagaaaactt
     1321 attttcagga ttcttaaaaa taaatttgat tatttaagag aatatattga aataaaattt
     1381 cttaattata taattagtta tgaacattct ttgcgttgtc atgcaatgaa tggttcatat
     1441 tttattgaaa aggagtggtt ttagtggaaa agctgatgaa atgggtcgaa gagaaacttg
     1501 tcccaccaat ggcaaaaatt ggtacacagc gtcatttatt agcaattcgt aatggggttg
     1561 tttcaacatt gtcattgatt ttgattggga cattttttat ggtctttatc aatttaccgt
     1621 ttcctggatg gaatgaattt attgcccctt attcggcaac gattgtttta ccctttagga
     1681 tgacgatggg attgatggct atttatgcaa cttttgttat gggttcggat ctagctaaat
     1741 catatggatt agatagtgtg acaggtggta tattatcttt aggaacgttc tttatgttac
     1801 aagttcctgt aaacgtttta actcctgaag aggcacctct tggctgggtc ttacctatga
     1861 gttctttggg agcatctggg atgtttgcgg ggatcttatc tatgattttt gcagtggaag
     1921 tatatcgttt ctttaaaaat cgaaatatta ctatcaaaat gccagaacaa gtgccacctg
     1981 ctgttgcccg tagttttgag gcattgattc caggtgcagt cattttaaca attacttggc
     2041 tgattcgaag cgtcgcgggc tttgatgtaa acgctgcctt attatcgtta ttcgagcctt
     2101 taaccaatat tttagggaat aatttgcttg gtgttttact accaatgttt ttgatccatt
     2161 tgttgtggtc atttggtatc catggcatga gtattgtagg ggctgtcgtt cgtccaatgt
     2221 ggctgatcat gttggatgag aatgcaaagg cattggcaga tggtacagct gccacccaat
     2281 taccctatat cgctcctgaa cagttttatc aatggacggt aacaattggt ggtgcaggag
     2341 caacgatcgt ggtatctgtt ttgtttatat tcttctgtaa atcaaaattc ttgaaagagg
     2401 ttggtcgttt ttctattatt cctggtatct tcaatatcaa tgagcccata atttttggag
     2461 ctcctatgat gttgaatcct tacatgttca tcccgttcaa cttagttcct ttagttttga
     2521 ctattatcag ttattttgca gtcaaattag aaatggtcaa tagatttact gttttgccaa
     2581 catggacatt accagctcca atcggcggtt atctttcagc aggaaatgac tggcgtgttg
     2641 ttatcttgat catgatcaac acattgatag catttattat ctattatcca tttgtaaaag
     2701 cttatgataa aaagatgctg tcagatgaac aaagtcgtgg agaaaacgaa gtccagactg
     2761 tctaagtttg gtttttagaa ggtgggaaac aatgtattgg ctgattttat tttttgtttt
     2821 tatcttttta ttaacggcta gtcaccttat attgaatatg ctagctacat atcatattca
     2881 aatcaatcga tggatttggg cactggcatc atttttgatc gtgattcttc caaagatcat
     2941 tgtaacgcat ttgaatgttt tgttttcatg ggggacatat gtactttgtg gaatttttgc
     3001 tatcaatttt atgatcgagc agcatagatg gtttgtcaca tcgaagcttt aaatgttcta
     3061 aaagtggttg taacagaagt ggttagcttc aagaaataag aagaaattta cgaaaattgc
     3121 ttcttaaatt tttgtgaaat gttggcttat ttttcgtttg gttgaactta ttaccgcaga
     3181 tgcaagcttt tgaccaccgt ttattcggaa tatgggactg tgacaaggct tttgtcacag
     3241 ccctttttta cataaagatt attattgttt taactgtttt ggcagaaaga aggaggaaaa
     3301 aatggaaatt ttatcagcaa tggatttaga attagtagat ctactaccaa agtgatcaag
     3361 tcaaatatag atttgggagc ttacaaccat atagtagggg ctgcagtatg gtgtatgaat
     3421 ggtaatatgt atgatgtgag tgtttattca aatcatgaaa cgtgtataga aataattgtg
     3481 ctgggaatag caatcagtca tggtgaacgt gacttttagt gtatcgttgc tgacgacata
     3541 gaacattctg acatgatcta ctcaatatgt ggggattgct ggcagtttat ataggagtat
     3601 gcatcggatt gcgagatcat tgtctcaaca gaagagggag gttgattatc aaagataaag
     3661 tggtaaatcc tacatttgat ttagtaattg ttgaggggac tagcggcaca aatctctaca
     3721 aagcgatgct tctagatcta tatgacacat tttaagaaaa tgtatttgcc taattattat
     3781 ggtctttctt ttgaagaaac gttgagatga tccaaccaga gaaaggtgtc aaagaatttg
     3841 actaagaata aatggcagac gctacctatg ctcatttgga tagcgtcaga tcttttattt
     3901 taaggatgat tttgtgtttt ttagacaccg taatttaagt aactctaact tttcaatcca
     3961 ctatttactt tttttgtatt ttcactgtct gggtctcgaa tagcgaaatt actgatgagt
     4021 ctctattaaa aaagcttttt ctacttgaaa ctatattggg tggttgctca ggaaggtttc
     4081 gatttcttga attgcttgtt gtggaccatt tattggaaaa acggcttgca gcgtgcgtgc
     4141 ctgctgacga atcttgggtg aagaaacttc gcgcagagca atctgcagtt ttctttcagt
     4201 cactggtttt gtgagggcga ttgcgtttcc gtattttaca caatagtcaa tattacagct
     4261 ttgctcaaat tgcatcccca caccgataaa cggtttgccg gattcacagg cagtttggac
     4321 ggtcccttcc ccgccgtgga taaaagaaaa atcaatgaat tcatgcaaaa gatgactaga
     4381 cagatattct gttattaaaa gatcttctga gtcagattga atgtcatcaa gcagatagtc
     4441 aataggtgca ataaccgcta gatccggttg tttttgtaag aagaacaaaa ttttgcgtaa
     4501 catacgcgga ttgcctgaac tacccattgc gaaatagata atacgttttc tttcttgttg
     4561 ctttcgctgt ataaaagtta gcacttcttc aggcaaggaa gtactgagct tagcaaaaat
     4621 tggaccaacg attcgataat tttccggcag ctgttcttct tccaaaaata gtttaggcgt
     4681 tgtgatgaga tttagatcag catccatcat ttgtaaactt gtttttggta aaggaagcat
     4741 ttctttttgt gcagcgtgtt tgaaggcatt ggggatccat cgggtattta ctatacgctt
     4801 ttttgtccag tgccaaaggg gattgcttaa agggccgaag aaacgtaact cgtggggcat
     4861 cagcgcatgc tctgattgaa aaaaaggttc actcaaagca tagggtttaa catatactaa
     4921 aggaatcttt ttattgcggg ccgataaaaa aattgtcaca ttcgaacctg tcacaatcag
     4981 tcgaggctca aacttagcaa tcagactctg ttcactagtg atacgagagg caaccatttc
     5041 tttggtaaaa gggtttttaa gtgtacgcaa ctggtcaaat tttatgattt gttcttcttg
     5101 ttttttcgta aggatcggcg ctagtaaatg atactcaaag ccactttctg tgatcaaagg
     5161 agcaaaagtt ttagaaaagc caaagaatac acagtaataa cggtctttca tagctttggc
     5221 aacctcaatc attcgtgttg tctcagctaa attgaatact gcaggagcaa aaagaattgt
     5281 cttcatgaaa tcctccttat tggaatgagt ctatcccttc gtttcttact agtatactaa
     5341 aaaacacagc gttcgagtag cctatgtata ttttgaatat taaactgtct ctcgctgttt
     5401 tcacaacttc taaatttaaa ttttgtaatc aagttgaaac tccaattttc aaaaaaacac
     5461 gcaacaattt tttgatcgat ttatatgttg tgccagataa atacgagaag ttgtgtcatt
     5521 aaaaataaac atgagaacat aaaaaaataa aataattata ttaattgata ggtgtgctgt
     5581 cg
//
LOCUS       DACDRN010000111         5258 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDRN010000111 DACDRN010000000
VERSION     DACDRN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.111"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(94..309)
                     /locus_tag="IWJ80_002643"
     CDS             complement(94..309)
                     /locus_tag="IWJ80_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP9462305.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            533..1333
                     /locus_tag="IWJ80_002644"
     CDS             533..1333
                     /locus_tag="IWJ80_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9462306.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1352..3220
                     /locus_tag="IWJ80_002645"
     CDS             1352..3220
                     /locus_tag="IWJ80_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9462307.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3213..3704
                     /locus_tag="IWJ80_002646"
     CDS             3213..3704
                     /locus_tag="IWJ80_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP9462308.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            3692..4246
                     /gene="srlA"
                     /locus_tag="IWJ80_002647"
     CDS             3692..4246
                     /gene="srlA"
                     /locus_tag="IWJ80_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP9462309.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4264..>5258
                     /locus_tag="IWJ80_002648"
     CDS             4264..>5258
                     /locus_tag="IWJ80_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP9462310.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         1852 a          833 c         1075 g         1498 t
ORIGIN      
        1 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
       61 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
      121 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
      181 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
      241 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
      301 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
      361 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
      421 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
      481 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
      541 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
      601 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
      661 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
      721 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
      781 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
      841 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
      901 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
      961 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     1021 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     1081 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     1141 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     1201 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     1261 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     1321 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     1381 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     1441 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     1501 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     1561 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     1621 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     1681 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     1741 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     1801 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     1861 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     1921 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     1981 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     2041 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     2101 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     2161 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     2221 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     2281 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     2341 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     2401 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     2461 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     2521 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     2581 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     2641 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     2701 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     2761 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     2821 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     2881 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     2941 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     3001 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     3061 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     3121 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     3181 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     3241 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     3301 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     3361 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     3421 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     3481 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     3541 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     3601 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     3661 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     3721 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     3781 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     3841 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     3901 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     3961 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     4021 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     4081 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     4141 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     4201 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     4261 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     4321 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     4381 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     4441 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     4501 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     4561 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     4621 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     4681 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     4741 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     4801 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     4861 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     4921 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     4981 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     5041 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     5101 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     5161 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     5221 atcattgctt gggtagcaag ttttggatta tatagctg
//
LOCUS       DACDRN010000112         5201 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDRN010000112 DACDRN010000000
VERSION     DACDRN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.112"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            25..696
                     /locus_tag="IWJ80_002649"
     CDS             25..696
                     /locus_tag="IWJ80_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9462311.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="IWJ80_002650"
     CDS             755..1195
                     /locus_tag="IWJ80_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462312.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="IWJ80_002651"
     CDS             1207..1875
                     /locus_tag="IWJ80_002651"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9462313.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="IWJ80_002652"
     CDS             1939..2562
                     /locus_tag="IWJ80_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP9462314.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="IWJ80_002653"
     CDS             2618..3004
                     /locus_tag="IWJ80_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP9462315.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="IWJ80_002654"
     CDS             3217..4608
                     /locus_tag="IWJ80_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP9462316.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1729 a          861 c         1095 g         1516 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacaga t
//
LOCUS       DACDRN010000113         5184 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDRN010000113 DACDRN010000000
VERSION     DACDRN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.113"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(81..683)
                     /locus_tag="IWJ80_002655"
     CDS             complement(81..683)
                     /locus_tag="IWJ80_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9462317.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(686..1126)
                     /locus_tag="IWJ80_002656"
     CDS             complement(686..1126)
                     /locus_tag="IWJ80_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP9462318.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            complement(1251..2189)
                     /gene="galU"
                     /locus_tag="IWJ80_002657"
     CDS             complement(1251..2189)
                     /gene="galU"
                     /locus_tag="IWJ80_002657"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9462319.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            complement(2204..3229)
                     /locus_tag="IWJ80_002658"
     CDS             complement(2204..3229)
                     /locus_tag="IWJ80_002658"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9462320.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            complement(3273..4103)
                     /locus_tag="IWJ80_002659"
     CDS             complement(3273..4103)
                     /locus_tag="IWJ80_002659"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP9462321.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            complement(4118..5056)
                     /locus_tag="IWJ80_002660"
     CDS             complement(4118..5056)
                     /locus_tag="IWJ80_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP9462322.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
BASE COUNT         1390 a         1139 c          885 g         1770 t
ORIGIN      
        1 attacttgtt ttagaaaaat aagagtaaaa aaggtgagag ctagtccgct cccacctttt
       61 tattctataa atttgtgatt ttagtgattt gtagatgaat ctgtcattgt atcttttatt
      121 tcttttttag cttctttcgc atcttctttt gccattttca tcatttcttt tgagtcatct
      181 gcaactggtt ttgcttctga cattccttca ttgacagtat ctcctgcttt accagcagcg
      241 tcttttacat caatcatgat atcttctgaa gtatctttag caatacttcc taattcatct
      301 gcaggttttt tcatatcttc tgccgttttt ttgaaacgat tagataagtc agctgattgt
      361 tctttaagat ttaacaagat atcgtcagat tcacttcctg cttcatctgc tgattcactt
      421 actttttgtt tgacactacc tgctagatca cctgcttgtt gagaaagatc ttgcgctttt
      481 tcttttgcaa tacctgttaa ctcagtcgat ttttctttcg ccatatctgt gtaatctgaa
      541 gccatatcca tcaaatcatc cgcttgggca gctaattcat cccgaagttc tttaccagat
      601 tttggtgcta acaataacgc ggcgattgct gcggctgtgc ctccgactac tgcacctaat
      661 aaaaatccac cttttttagc catttttatt ttcctcctgt cgtttcagta tgttttttgt
      721 ttttaaaaaa tttcatcgct gtcccaccga cttttccggc aaccgtcgct ttagctgtcg
      781 ttttaccgat tccgccgact ttcgtcgcga tgtttttact agaatgatta agttctgata
      841 cactttcact taaatcagca actgctgtga acaatggatc gatcgttgcg acttttccat
      901 tgatatccaa gagcagttca ttacttttga ccagtaagcc ttctacttgt ctagacaaaa
      961 gatctacgtc ttttgtgacg atttcgatcg tcgtatttgc ttctgctaca gtctgatcta
     1021 ctttttctaa agttttagaa acttgcaaca acacacgaac gataaaaacg acaagtatcg
     1081 caaaggcgat tgctgcgatc aatcctgcaa tttctcctcc tgacatgatc aaactccctt
     1141 cttgtctctc ttttctataa ggatagcatt ttcgtgtttt ttaaacaaat aaaaacgagc
     1201 ttcaactact ttttattcaa ttagcagctg aagctcgtta gaaaaaaggg ttattttttc
     1261 ttttcttctt tgatttctgt tattcctttt ggattatgat cgatcttgct tccttctgct
     1321 tctaatgttt tacctaaaga caggatgtac tcacgtaaag gtgcacctac ttctggatgt
     1381 gttagaccat attcaatact tgttttcata aaaccaaatt tgtcaccgac atcgtaacgt
     1441 ttacctgtga atttacgggc aaatacgcgt tgtgttttat tcaatgtatc gattgcgtca
     1501 gttagttgga tttcatttcc ggctcctggt tgttgctcgg acaatacatc aaaaatttct
     1561 ggagtcaata agtaacgtcc aataatagct aaatcacttg gtgctttgct tggatctggt
     1621 ttttcaacaa agtttttgac attgtataac ccatcttcca atacttctcc tggattgata
     1681 attccgtatt ttgaagtttc atcatgagga acttgcataa cggcgatcgt agaagcatga
     1741 gtacgttcgt aatcatcgat taattgctgt gtcaatggta cttcgtcttc cattatatca
     1801 tcgcctagca taacgacaaa tggttcattt ccaataaagg ctcttgcttg aagaacggca
     1861 tgtcctaaac cctttggatg agattgacgg atgaaatgca agttgacatc tgtcgtttct
     1921 tctactaatt tcaataattc ggtctttcct ttttcactta ggtttgtttc taattcaata
     1981 tttgaatcaa agtgatcttc gattggtcgt ttagcttttc ctgtaacgat caagatgtct
     2041 tcgatacctg atttcaacgc ttcttcaacg atgaactgga ttgttggttt atcaacaatt
     2101 ggcaacattt cctttgccat agcttttgtt gctggtaaaa atcttgttcc taatcctgct
     2161 gctggaataa ctgctttttt gactttcata tacattcgct ccttcaaaaa attgttaatg
     2221 agaattcatt ttcttttttc ccatcacgta acatgatttc ttttgcggct gttttgattt
     2281 ctttttgttc atacagcact tcatagattg tttgagtgat gggcatttcg atttccatta
     2341 tttgggctag ttccatcgct gcttgggtag tattcacacc ttcaacgacc attcccatat
     2401 tggctagcac ctcttctagg ggctgccctt gtcctaacaa gttgcctgcg cgccagttcc
     2461 tagaatgaac acttgtgcaa gtaacgacta gatcgcctac tccgcttaat ccgataaagg
     2521 tcagcgggtt ggcacccatt gctacaccta aacggctgat ctcagctaat ccgcgcgtca
     2581 tgattgctgc ctttgcatta tcgccaaacc ctaagccgtg aatagcgcca gcaccaatag
     2641 caataatatt tttcaaagct gcccccatct ctacaccgat cacatcagga ttggtataga
     2701 tccgaaaata attattcatg aacagctctt gtacatattt tgctgctttt gcatctgtac
     2761 ttgcagctgt gatcgtagtg atgtcatgtt tcgctacttc ttctgcatgg cttggtccag
     2821 aaagaacaac aacagctttt cttttttcta ctgggatttc ctctaacaag atatcagaga
     2881 tccttttatg tgtcccctgt tctaatcctt tacttgcatg gatgatcact ggctttgtat
     2941 tcaatttagg cactagttct tttgcaacag aacgtattgc tttcgttgga atgacgaata
     3001 gcaccgcatc tgcgtctttt actgcatctt ccatattggt gaatgcttgg atggaatcgg
     3061 gtagacaatg atctggtaaa taacgttgat tcgtgtgttg ggtgttgatt tcttctactt
     3121 gtgaagcacg atgtccccaa atccgaacgt catgtccgtt ttcggcgagg acttgtgcca
     3181 aagcagtacc ccaagagcct ggtcctaaaa ccgctactct ataattcatt ttaccactcc
     3241 tgaaattttt ttcattttta acgctctaaa aatcagataa tttgttgatt ttttcctaat
     3301 gagcggtcat aatccggtaa atttcctttt tttctgcgcc aaataaatag aatcaatgta
     3361 ccgacaaata acacagcaga taataattga gaaatacgga tcacacctaa aaagtaaaga
     3421 ctgtctgtac gtaatccttc aataaagaaa cgcccgaaac tataccacat tagataacat
     3481 aaaaggactt cacctttttt caaaaggtgt ggtttctttc gtaaaaacaa aagcaaaata
     3541 aatccaatca aactccaaag tgattcataa agaaatgttg gttgacgata gatcccttcg
     3601 atatacatat tatcaatgat gaattgcggt aaatgcagac tttctaagaa gcttcgcgtc
     3661 gtgtctggtc catatgcttc gtggttcatg aaatttcccc aacgaccgat tgcttgtgct
     3721 aataatacac ttggtgcagc gatatctaaa aaagtccaca aagaaataaa atgatattta
     3781 gtaaaaaaat acagcgtgat cgcgcctgca atcagtccgc cataaatagc taagcctcct
     3841 gaacggatat tgaatatttg gattggatca gctatatact ggggaagatc aaataaaaca
     3901 taatataagc gagcgccaat aatggagata ggcaatcccc acaacataaa atctgtcaca
     3961 tcatctgctt ttaatcctac ccgaacagcc tctctgctgc taagccacat ggcgataatg
     4021 atacctgata cgatgatcaa tgcataccag tagataggaa tccctacaag ctcaaaagca
     4081 actcgattga tttgtgctaa catattgtca ctccttgcta gtcgttgcct gagttttctt
     4141 caatcaagcg acttaatctt tcttcaaatg ttttcgttgc gtcaaagccc attgtacgtg
     4201 cacggaaatt catcgcggct acttcaatga tgattgcaac gtttcgtcct gttttgacag
     4261 ggatttttat ttgtggaatg cctacttccg cgatttcaac ggtcgtgtct tcagagccca
     4321 gacggtcgta tttcttatct tttgcccatg cttccagata aacagccaac tggacttgca
     4381 tagagccacg taccgcactc gcaccaaaaa gattcattac atcaatgatc ccgattcctc
     4441 ggatttcaat cagatgttcc aggattttag gcggttctcc gatcaccgtt aattcatctt
     4501 gctgataaac atctactcga tcgtctgcga tcaatcgatg accgcgtttg atcaactcca
     4561 acgctgtttc actttttcct atcccactgt caccttgaat cagtacacct aatccgtaaa
     4621 catccactaa aacaccgtgg acacttgttc gaggggcaag gcgaccatct aaataactgg
     4681 ataattctcc taataagcga gaagttgaga tgggagaacg cagtacagct atcccatttt
     4741 catttgctgc ctggatcatt tcttctggag ctggtaatcc tcttgaaata ataaatgcgg
     4801 gcatatcttc actacataat cttctcatga tgatcaaacg ctcttctggg atcatccgtt
     4861 ccgcaaaact gatttcttta ctgccaaata gctgtaaacg attatgggaa taataattaa
     4921 aataaccagt caactctagt cctgggcgtg agatgtcccc agttacgatg acacggtcta
     4981 agcttttctc gtcgcctgtc acgatttcca aagataattt tttcactaat tggctaactt
     5041 ttacaacttc tgacattcat gctacctcat ttctattaga gtgttctcta tcattttatc
     5101 attgtctagc caaaagtact accaattaaa aaaaacgttt ttatttaatt accaatgtaa
     5161 tagaaaacgc attcctgatt ctaa
//
LOCUS       DACDRN010000114         5180 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDRN010000114 DACDRN010000000
VERSION     DACDRN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.114"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            223..1200
                     /locus_tag="IWJ80_002661"
     CDS             223..1200
                     /locus_tag="IWJ80_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP9462323.1"
                     /translation="MAFTAPFPLTYNPNGWDIRNPYDILPELVEDLRSQVDVLVLMSH
                     LGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQIAAAGKYGQYVGEVHLTIDE
                     EKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELLKEKEVADLPFSLSLDIFNEH
                     SFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQDQLHTALPHPMHLLNVTLGGN
                     DLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNGITYDSINHQVFWKNKPIDLN
                     KNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVVGDYLKAHYPIK"
     gene            1237..1914
                     /locus_tag="IWJ80_002662"
     CDS             1237..1914
                     /locus_tag="IWJ80_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP9462324.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            1922..2686
                     /locus_tag="IWJ80_002663"
     CDS             1922..2686
                     /locus_tag="IWJ80_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP9462325.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            2688..3347
                     /locus_tag="IWJ80_002664"
     CDS             2688..3347
                     /locus_tag="IWJ80_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP9462326.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(3377..3886)
                     /locus_tag="IWJ80_002665"
     CDS             complement(3377..3886)
                     /locus_tag="IWJ80_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP9462327.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(3998..4996)
                     /locus_tag="IWJ80_002666"
     CDS             complement(3998..4996)
                     /locus_tag="IWJ80_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9462328.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
BASE COUNT         1690 a          927 c         1015 g         1548 t
ORIGIN      
        1 caggaaaata tcttgctgat gaatgaagtt ggttatgatg cagcgacgat aggaaacaat
       61 gaaggcgtag gaaattccaa agaagagttg aatcatttat acgatcatgc tgagtttgat
      121 atcatcctgg ataacttatt cgataaaaat acgctccagc caccggcatg gacgaaacca
      181 tataaaatca ttacaacgaa acaccagacg aaaatcggac taattgcatt tactgctcca
      241 tttccgctta cttataatcc gaatggctgg gacatacgga atccttatga tattttgcca
      301 gaattagtcg aagatttacg ttcacaagtg gatgttttag ttttgatgag tcatctcggg
      361 attcaggatg atttccaaat tgcaaaagaa ataccagcaa ttgatgttat tttaggatcg
      421 cacacccatc atttattcag agaaggtcac gtaatcaatg atgtccaaat agcagccgct
      481 ggaaagtacg ggcagtatgt cggtgaagtc catttaacca tcgatgaaga gaaaaaaatc
      541 cttaagcatt cagccaaagc catccctacc gaaacaatga cagcttttac agaagatgaa
      601 caagaaataa atagttatct agaacgtggg catgaattat taaaagaaaa agaagtagca
      661 gatttgccat tttctctgag tctagatatc ttcaatgagc attcgtttat ccaagtagcc
      721 tttgaggcgg tgaaagaacg aggaaagacg gaagcagcaa tattaaacag tggtctgttt
      781 ttaactgaaa ttccaaaagg gcgggtcaat caggatcagc ttcacactgc gttgccgcat
      841 ccgatgcact tgttaaatgt cacattgggt ggaaatgatc tgatccgctt agttttagaa
      901 atagaaaaaa atcgtaactt tttacgtaac tatcctatga agggaatggg atttcgcggt
      961 aagatttttg ggcagatggt ctataatgga atcacatatg attctattaa ccatcaagtc
     1021 ttttggaaga acaagccaat cgatctgaat aaaaattata cttttactac cgtagatcat
     1081 ttgatgtttg ttccattttt tccaacgatt gagattgctg gaaaaaatga atttttgttc
     1141 cctgaattta tccgaagtgt ggtaggagat tacttgaaag ctcactaccc aatcaaataa
     1201 aaacaaggta taataaaaaa gataggtggt gaacgcatgt ctgcaaaaca aaatgaacag
     1261 cagaacgttg agcataaaac agaaattgct aagaaaaaat catcagctga acaggaaaca
     1321 acagtcaccg aagaactgac agctgctttg gaagaaatcg ttgaggagcc taaagcagaa
     1381 aaagtcaaag gcgagctagt gacactgtta gatgacagta attttttgat aggtgaaaga
     1441 cattatcggc ttgtgtccga ttatcgtgaa ggatttaatg cagaaaagct tggagaacgt
     1501 tacagtgatg tactagcccg atatgattat atcgtaggag attggggtta tgaacaacta
     1561 cgcctaaaag gatttttcca agcggataat cgacgggcgc atccagatca gcgaatcgat
     1621 tccttggaag actatcttta cgaatattgc aattttggtt gtgcgtattt tgtcatcgag
     1681 agaatcggcg gtaaaaaaga aaagacaact cagcgccgaa aaaagaaaaa aaaccataat
     1741 cgtgcgtata tcgatgagaa aaaaggacca gtcgcatcta atcgcaaaaa accggttatt
     1801 aaaaatcgaa aaacagagtc agcaaaaacc tctgaaaaag taaaaaaaca agaacaacct
     1861 aaacaagtag aaaaaacaaa aacagcattt acgatcagaa aaagggaaga gtagtggatg
     1921 aatggactat aaaggatatt taattgattt agacggaacg atttatcgtg ggacacaacc
     1981 cataccggcc ggtaaacgat ttgtggaaga attgcaaaaa agaaaattac ctttcttgtt
     2041 tgttaccaat aacacgacta agtcaccaga aacagtagct aatcgtctag cagatgagtt
     2101 tgacattcat gtagctccag agacagtcta cacagctact ttggcaacaa tcgattttat
     2161 gaaagcagat ggaaaaggga aaaaagtcta tgttatcgga gaagcaggat tgattgactt
     2221 gattttagct gccggattta catgggaaga agaggctcct gactatgtag tggtaggctt
     2281 agataattat ctgacctatg aaaaagtagt taaagcaaca cttgctattc aaaaaggtgc
     2341 tactttcatt ggaacgaacc cagataagaa tatacctact gaacgcggcc ttcttcctgg
     2401 agcagggtca gtcatctctt ttgtagagac agcaacccaa acgccgccga tctacatcgg
     2461 aaaaccagaa gccatcatta tggataaagc cgttgaagta ttagggctgc aaaaagaaga
     2521 agtaatcatg gttggagata actatgaaac agatatccaa gctgggatac gcaataatat
     2581 agatactttg ctcgttttat caggttttac aaaagaagaa gatgtacctg ggttgcctat
     2641 cccagctacc tatgtgaaac aatcactgga tgaatggagc ttctaatatg aaaaattcga
     2701 gatggcagtg gatggaatat gctggattgt tcagcctatt tctgacactg atcagtctgg
     2761 cagttggtgt gacgatcaac tttcgaccac tatacgtatt tgatatcgga catttgcaaa
     2821 ttttggatta tacttcttta gatcaagaga cgttactgaa aaattttgat cacctaatga
     2881 attatctcaa taatcccttc aaaaccatcc tcagcttgcc agatttccca gtttctgcta
     2941 gcggagccca tcacttttat gaagtgaaaa ttttgttttt agtggattat gctgtgtttt
     3001 tcatcacttt gatcccgagt attttgttta tcaaatattt gcaaaagaat gatcggcttt
     3061 ggcggttgat tcgtccgttt caaatcggca tgttactgcc agttgttttc ggttttttca
     3121 tgatgatagg atttgatcgc ttttttatcc tttttcatga aacgtttttc aacaacgatg
     3181 actggttatt cgatcccgtt actgatccaa tcatcaatgt acttccagaa gaatttttca
     3241 tgcatagctt tatcttattt tttgtattat tagaattatt ttttgctgtt tttcttttct
     3301 taggaaaaaa tagcttgaaa caaacaaaaa agaaagaact cgtctaaata gacgttttct
     3361 ttcttttttt gttgaattat tgagtaaggc gacttctttt ttctggttct ttgtgtgcta
     3421 atcgactggc agcagcagcg gcgatcgctc cgacgatatc atctaagaag gtatggacat
     3481 gaacgccatc gtggctgttt agttctttta aaataccagg tttcaccttg tcgatataac
     3541 cataattcgt aaagccgatc gtaccgtaaa cattcacgat tgacaaggct aggatctcat
     3601 cgataccgta aaggccttcg tcatcttcga taatatgttg caacggactc atcaattttt
     3661 tcttttctgc taatatatct aattggattc cagtgataat ggcgttatgt acttcacgct
     3721 ttgttagcac gctgttgaca ctttcagtac aatccgccaa agtcaaatca gaaatatagt
     3781 ctttttgtaa aaacatgaca agttgtgcga tatcttctac agttacgcca cgttccttca
     3841 atagttctct tgccttttct tctaaagtct cagttttgac gaccatatta ttgctccccc
     3901 tttatttttt ctttctctat ttcatgttaa cggaacatga aaaaaatgca agaaactgct
     3961 ttcgggttta acttgaaatg caaagagata taaaaaatca actaggacga agatttttat
     4021 cgtataagct tgtgctgtga ccaggctgag ttcgttcctt tggattaata tagtgtaaag
     4081 cgttgttcac tgcagtaggt gcttcaccga gcccagtagc aatcagtttc acttttcctt
     4141 cataggtaca gatgtcacca gctgcataaa cgcctggaat agaagtagac atatctgatt
     4201 ttacagtaat ggcatttctg gtactatcaa gtccccaaga agaaagatgt tctaaattag
     4261 aagagaatcc ataattgacg atcagagaat caatcatcaa atcaatcgtc tcgtcacttt
     4321 tcactttttt taaccggata tctgtcagtt ccccattatt gcctgataat ccatcgatga
     4381 gatagggagt aagcaagttg acagatgaag atttcaagcg tgatacacta tgttcgtgcg
     4441 cacgaaattc cgggcgacga tgaatcaagt aaacttcgct tgcaataggc tctaacatca
     4501 acgtccaatc gatagcagaa tcaccacctc cagcaatagc gacttttttt ccagcatact
     4561 tcatcaaatc attcacgaaa taatccaatc cgtggttttc aaatgattca gcattatcta
     4621 aattcaattt tcttggctgg aatgaaccat ttcctaaagc taaaatgact gctttggaat
     4681 aatggatgcc tttatttgta gtaatctcga taacttcgtc ttcacgagta agcgagagaa
     4741 cttcttcttt taaatggaat gtatggttaa aagtagttaa ttgtttttct aattgatcga
     4801 ttaattcact tgcttttatt gctggatacc caggaatatc gtaaatatat ttttcgggat
     4861 acaaagttgc taattgacca ccaagttgag gtagactgtc aatgattttt gttttagctt
     4921 ggcgcattcc tgcgtaaaaa gcggcaaaca tccctacagg acctgcaccg atgattgtaa
     4981 tgtcatagat ttccataaaa attcctccct tttcacacta tagcagaatt taattgagaa
     5041 tgcttctcaa aaatgtttga attcgaaatc gaaacggtaa gttatagaat gtatctagtg
     5101 atgaaaaata aaagaaaaca aatctagtat ttttacgaga ggttaggtat tatgttatta
     5161 ttattatgaa gtatgcaaat
//
LOCUS       DACDRN010000115         5034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDRN010000115 DACDRN010000000
VERSION     DACDRN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.115"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="IWJ80_002667"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="IWJ80_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP9462329.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="IWJ80_002668"
     CDS             complement(2064..3062)
                     /locus_tag="IWJ80_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP9462330.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="IWJ80_002669"
     CDS             complement(3288..3941)
                     /locus_tag="IWJ80_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP9462331.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="IWJ80_002670"
     CDS             complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="IWJ80_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP9462332.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1492 a         1036 c          766 g         1740 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcta attgtcgtac
     4321 atcgcgcggt aggaaccgac gaatttcatc ttcattaaaa ccaacttgca gacgtttatc
     4381 atcaatcatg attggacggc gaagcaatcc aggattgttt tgcacaagtt ccaataattc
     4441 ttgtaacggc agatcatcta aatccatgtt cagtttttgg aatactttgg aacgtgtcga
     4501 gatgatttct tctgttccgt cttctgtcat ttgtaaaatc gcttttaatt cagaactgtt
     4561 caatggttct gaaaaaatat tacgttcaac aaatggaatc tgatgttctt gcaaccacgc
     4621 acgagccttt ctgcaggaag tacaacttgg ggaagtatat aatgttaaca acgtgcacca
     4681 ctccttttcg tgataagaat cacttttttg taacagaaga tcataataat ctcccatctg
     4741 tttctattat acataatcaa cttttaaata gctacctttt tttcataatt tttttaaaaa
     4801 aatgaaagtt atctaaggtt aatatacagt aaaaccagca ttctcgcttt ttttatcatt
     4861 taaaaattag agttttcaaa tctaattcaa attttctaaa catatgaaac agcgtataaa
     4921 actgtactat tattttatct caaaagctaa atgaaaccga tttcttaaga ttttttatgt
     4981 tttttatttc tttaatagta atgttttctg tcattttaaa taatactgtt cttt
//
LOCUS       DACDRN010000116         4922 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDRN010000116 DACDRN010000000
VERSION     DACDRN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.116"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..191)
                     /locus_tag="IWJ80_002671"
     CDS             complement(<1..191)
                     /locus_tag="IWJ80_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462333.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQ"
     gene            complement(401..485)
                     /gene="ffs"
                     /locus_tag="IWJ80_002672"
     ncRNA           complement(401..485)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWJ80_002672"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(508..1023)
                     /gene="tadA"
                     /locus_tag="IWJ80_002673"
     CDS             complement(508..1023)
                     /gene="tadA"
                     /locus_tag="IWJ80_002673"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP9462334.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            1130..1987
                     /locus_tag="IWJ80_002674"
     CDS             1130..1987
                     /locus_tag="IWJ80_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP9462335.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(2068..2403)
                     /locus_tag="IWJ80_002675"
     CDS             complement(2068..2403)
                     /locus_tag="IWJ80_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462336.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(2470..3840)
                     /locus_tag="IWJ80_002676"
     CDS             complement(2470..3840)
                     /locus_tag="IWJ80_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP9462337.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(3918..4454)
                     /locus_tag="IWJ80_002677"
     CDS             complement(3918..4454)
                     /locus_tag="IWJ80_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9462338.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            4700..>4922
                     /locus_tag="IWJ80_002678"
     CDS             4700..>4922
                     /locus_tag="IWJ80_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462339.1"
                     /translation="MTFHSQFLSNYIQKQNKKEVFMLIEARKKELLVLQMVIQKNNCS
                     LQELSNDLHIPKRTIKELIRKINVSLKQFL"
BASE COUNT         1484 a         1047 c          740 g         1651 t
ORIGIN      
        1 tcctgtccat tcgttgcttc tgacaatgga tgccaacgat ccacaaaatc acctagatct
       61 actgcaatcg ttgtcgtttt ttctttcttt tcgttttctc gtttacgttg atccaaaaac
      121 cttctgatcc ttggccaatt ttctaaatga gaatgcagat cattcgtatg taaaatcact
      181 attttttcca tgtaccctct cgcctctctt tatctttatc ataccatagg cagctttttc
      241 ttccattgaa acaagctcta tttcagagtt tttcctgcct tgaagcaaat tgtataagta
      301 caatattcga taaatcagtc tggctaaaaa atcaggaaca ttcattttta cagtactata
      361 aaaataaaaa aaacagaccc gaaagtctgt tgtcgtttca ttataatcag acacatggcg
      421 gaacctgctt agtgctgctt ccttccagac ctgacacgct tcacaagccc accattgtcc
      481 tagcctttcg gcaaagaata gtatacctta tttttcattc gttgaccagt ctttttttaa
      541 tttctttttt cgctggcgga gctttcggaa aaagtcagtc aaaatcatcc cacattcgtc
      601 ttctaaaatg ccaccttcta catatgcttg gtggttaaac cgctcgtcct ccaaaagatt
      661 catcaaggta ccggctgttc cgccttttgg atcatacgcg ccgaaataaa cttctgctac
      721 acgagacaaa atcatcgcgc cgctgcacat aggacaaggc tctaaggtaa caaaaagtgc
      781 agcatcttcc aagcgccagc ttcccacttt ttcacaggct tccttgattg caagcatttc
      841 tgcatgtgta gtagcatctt gcgtggtttc tctaaggtta tatcctcttc caatcacttt
      901 tccatccaat acaaccactg ctccaatcgg aacttcatga agcatttctg ctttctttgc
      961 ttctgccaat gcaaggcgca tccatttttc tttttctact gcatcaagat ttgtatttac
     1021 caattaccct tccctgcctt tgttttttct tgttttactt tagcatatga taaaaaggct
     1081 gtaactaact tttttttcta tggtaaaata aatacaagag gtgaacaaga tgaaaatgga
     1141 taaattactt gctctttacc cacaagcaca gaaaaaaaac tctccagcat cagaagcgga
     1201 tatcctttct tttgcaattg atgatgcttt tatctggatc aaacaagatt cattatctca
     1261 acaagaaacc agccttttga aagcgttatt ccctgtaatc aacgatcaaa aaaaacaccc
     1321 atggtatcac tatcttttta aagatgcttc ttgtgttcaa gaaaaaagct ttcgtttgat
     1381 ccagcttcac gtcgaatcaa aaggagaatt cttaaaaaaa gaatggcaag atactatttt
     1441 ggaattattt cgctattctg ctgatttctt tttttataca gacacagatg cgctcttagt
     1501 cgaacaaata aaccgtgatt atcttgatgc tactgaaatc aacggtattt ttctatcttt
     1561 agatgctgat tttgatacga caacgtctgt attcgttggt tctttccatt cacctggcac
     1621 cgacctcgtc cctttgtttg ctgaagaaag aaaaattttt ttaacagagc aaaacaatct
     1681 ttatactcac agccgtactt tttctatgca agatgtggct ttacattact atacaaaaga
     1741 tactgtaaaa gatagtaagt tgatcaaaag ttacatagaa ctgatcaaac aaaatgaaat
     1801 ggaagtgatc attcaagaat tatggaatga attaggcaat gtcagctctg ctgcaaaaac
     1861 actgtttctt catcgtaata ctctaaaata tcgaatcgaa aaatttcaag aacagtctgg
     1921 tttcaatcta aaaaaagcca acgatttact gttttgctat cttttattgc ttcatgaaca
     1981 taattaaaga tagattaaag gggttgtgac aataattttg tcacaacccc ttatttcgaa
     2041 tgaatagtgt tcaaaagcta gtttctctta catcattttt tcaacaaaat gcattagccg
     2101 gtcttttaca tcttcggttg aatctttgat tgaatcacca taaacagaga ttttcttttt
     2161 tcctgatttg atttttgcta acattcccat caaagaatcc aagtcactat ctgaaagacc
     2221 atctacgatt tctagtaatt tttcatttcc atcaaatttg tcatagacga atttttttac
     2281 tttcgtccga gtagtcatat gatggatctt ttccattact tttcccgata caacgactgc
     2341 tgtcgctaca ctggcagcgg ctgctacgct taatccgatt gttacttttg ttgatgtttt
     2401 cataagtatc agctcctttt tatctatctc catcatagca tagacaaaaa aataaacgaa
     2461 ctaatccact cagtgaaatc tcaatccttt agggaaaaag ttctttactg ttccttttac
     2521 ttgtttccca aatccgacag gtatcttatc taatactagc aaaacccatc cttgattccc
     2581 atcacgttga aatgtttctc ctgctgtgta agattgccat tccttttgag tgatcggcaa
     2641 gcaaggtata ttctctatct tttttgtagc aagcgctaat gcatagctag gttcaaatcg
     2701 attctttttg aaatctccta aatgcaaacc tgtacgtacg acttttaaac catcgagaga
     2761 aggggctaac tcaggaactt cccaaagatg gtcgttgaac actaaaagtc ttccagtagc
     2821 ttcataatga aaatcatttg aaaactccgt ccagagtttt tcctgctctt ttgtcatttg
     2881 cactttactt ttttttcgtg tcttcttttc tttatgcatc tgattttgtc catgaaaagt
     2941 cagctttgca acaaaatgtc cttcaccctg atctttatga ggccatatac gtattgtttt
     3001 ttccaaacca gctacgcttc cccattcaga acgtcctgaa ctaacggatt gagtcaaagg
     3061 aatctcctca atcgttactg gataattttc tacaagccag ctaatgatct cttcattttc
     3121 ttctggtgca aacgtacaag tcgaataaat cagttgtcct ttatttttta gcatttttat
     3181 tgctgaagac aaaatctctt gctgacgttt ttgacagtat agcggagatt cttctgtcca
     3241 ctcttttatt gcatttggat cttttcggaa cattccttct cctgaacaag gagcatcaac
     3301 cacgatccga tcaaaaaagc ccgaaaaatg agggacaagt tctgcgggtg catgatttgt
     3361 cactattgcg ttcgaaactc cccatcgttc tatattttct gataaaatct tcgcacgttt
     3421 cggaaagatc tcattcgtca ctaataaccc tttccccttc atttgagcag ctaattgggt
     3481 tgatttacca ccgggagccg cacacaaatc caaaactttt tcaccgggtt ttgctgcagc
     3541 agctgttcca acgatcattg cacttggttc ttggctatat tcatacccag cttgatggag
     3601 gaaagacttt ccattgacag tgccaagaaa tccctcattg ctgtagggag cgggttgtaa
     3661 ttcttcgcta tggtaagttt ggaccatgtc caatcctgcc ggttttagtg gattccaccg
     3721 aaatcctttt ttcacgcttc cttgttcaag cgcagaaaaa aaatcggatg cttcttcgcc
     3781 taaaagaaga cgatattttt tgataaattg ctgtggtaac gttgtcgctt cctccttcat
     3841 tccaatgttt cgttagacat tttacaatct tttttacgaa aaataaaggt ggctgatcta
     3901 ctttttcatg ttgctgccta aggtgactgt tcctttaaga tattttccaa gtattcatcc
     3961 gtcacctcgt ttttatccca aacagcgttc atcatttcaa gaaaatcttg agaagaatca
     4021 tctgagcgca aacttcccaa tgcattaagc cataccaatt caagcgactt ttcttctttg
     4081 atcttcgtgt gacctttgac atatgagaaa aatggccgct taaatagcga atccatgtct
     4141 aaatcagatt ttgcaatgat ttcacgaata ctttcatgga tagaaaaaaa atctttcaat
     4201 aagtagacaa tgcttaaaac atcatcggaa ctgcattcag ctaagtagtt ttcaaaataa
     4261 acagtgattt cttctaatat cttctctttt aacccgctga ttgatttgaa atgtgtataa
     4321 atcgggtatg tagaacaacc gcagaactct gctaactctc ttgcagtaat tgacttcatt
     4381 ccatattctt ccacgtatac catacttttg aacagcaaat attctttact ataatttgtt
     4441 actctcccca tcattcttac ctccgtctct tttcatctac ccataaataa ataacataaa
     4501 taattctaaa aatattattg ataattatca aaacatattt agttatatcc attaaaaatc
     4561 aatttttccg ccttaaaaac aagaatatct tgacaaaata ccctaggaaa taaacaatag
     4621 aataaacaag gtaacaaaaa aattaaataa cgttatataa aaaagaaggc gacactctgt
     4681 gagaataaca cagactgaaa tgactttcca ctcacagttt ttgtctaact atatacaaaa
     4741 acagaataaa aaggaggtat ttatgctaat tgaagcaaga aaaaaagagt tacttgttct
     4801 acaaatggtc attcaaaaaa ataattgttc tttacaagaa ttatccaatg acttacatat
     4861 tccaaaacgg acaatcaaag aactgatccg aaaaataaat gtatcactca agcaattttt
     4921 at
//
LOCUS       DACDRN010000117         4920 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDRN010000117 DACDRN010000000
VERSION     DACDRN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.117"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..477
                     /locus_tag="IWJ80_002679"
     CDS             <1..477
                     /locus_tag="IWJ80_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9462340.1"
                     /translation="YGKKELASVGMFFSAIVYLILFLIKASNMYVFLILSSVGYMGLG
                     VFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVGGWTLSIIGYD
                     SLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENCELLKQKHK"
     gene            487..1281
                     /locus_tag="IWJ80_002680"
     CDS             487..1281
                     /locus_tag="IWJ80_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9462341.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            1294..3060
                     /locus_tag="IWJ80_002681"
     CDS             1294..3060
                     /locus_tag="IWJ80_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP9462342.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            3083..4891
                     /gene="uidA"
                     /locus_tag="IWJ80_002682"
     CDS             3083..4891
                     /gene="uidA"
                     /locus_tag="IWJ80_002682"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP9462343.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT         1785 a          590 c          991 g         1554 t
ORIGIN      
        1 tatggaaaaa aagaactggc atcagtagga atgttctttt cagcaatagt atatttgatc
       61 ttatttttga taaaagctag taatatgtat gtattcttaa ttcttagttc ggttggctat
      121 atgggtttgg gtgtgtttaa tactgttatt tgggcaaata ttactgatgt tattgatgat
      181 caagaagtaa agtctgatca aagagaagat gggaccgtat atgcggtata ttcttttgct
      241 agaaaaatag gtcaagctct agctggaggc gtcggaggtt ggactctttc tattataggc
      301 tatgactctt tagcaaatgt acaaacggga gatgttttag aaaagttata taacgcttca
      361 acattaattc catcaatttg ttttctgatt gtaggattaa ttttattttt cctttatcca
      421 ttatctaaag ataaagtctt agaaaattgc gagcttttaa aacaaaaaca taaataggag
      481 agaaatatga ataattggat attttgtatt gattcagatg gctgtgttat ggatacgatg
      541 acttataaac acaatctctt tttcggacca attgctgcag agatttttga tgttcatcat
      601 agagaaaaat tcctacagga ttggaataat attaatttat attcagagac tcgtggaata
      661 aacagatttt taggtttagt taaaacatta gaaaaaaatg atgtaacatc gattaatcga
      721 ttaaagcact gggtatctct aacaaaagaa ttgtctaatc catctttgaa gagggaaata
      781 gaatattctc agtcagagga tttattaaaa gctttaaaat ggtcggaaga agtcaatcaa
      841 cagattagtc aagcaaaagg attagataaa ccctttttaa gtgctagaga tactataaaa
      901 gctctgaaaa aatatgggaa gataattatt gtaagctcag ctaataagga agctgttcaa
      961 gaagaatggg aaagacatga attattatca ttagttgatg aactgtgttg tcaagataaa
     1021 ggaaaaaaag aagatattat tcgaagtgta attgaaaatg gttgtgattt ggataagata
     1081 ttaatgattg gtgattctcc tggtgatcta gaagctgcaa ataaaaataa agttttcttt
     1141 tatcctattt tggtcaataa agaaaaagaa tcatgggaaa acttaagaac tgatgtttta
     1201 tttaaatgtt tatcgggaga ttatgtagat attgaacaaa aatatataga tactttttgg
     1261 aaaaatctta ctaataagta gaggagaaag aaaatgacta taaaagtaga tttacgaaaa
     1321 aaaccatata atttagatga agatgctatt tcttggattg aagagacgat tgattctatg
     1381 actttggaag aaaaaatagg tcaattgttt gttaatatgg gatctagtcg tgataaaaat
     1441 tacttgagta atatggtcaa tcagtaccat attggagctg tacgatataa tccaggaact
     1501 gctgatgagg tatatgatca aaactatatc ttacaaacag aaagtaaaat tcctctatta
     1561 attgctgcta atacggaagc aggtggtaac ggagcttgta cagatggtac tgaagttggt
     1621 gtagaagtaa aaattggtgc aacaaatgat gcgaaatatg cttatgaatt agggagagtt
     1681 tctgggattg aagcatcggc tattggatgt aactggagtt ttgctcctat tgtagatatt
     1741 aatagaaatt ggagaaatcc tatcatttct agtcgatctt ttggacaaaa ccctgataaa
     1801 gttttagaac ttagtttagc ttatatgaaa ggtattcagg agagtggaat tttgcctgca
     1861 gctaaacatt tccctggtga cggtattgac gaacgagatc agcatctatc ttttagtatt
     1921 aatagtttag gtacggaaga atgggataat tcttttggaa aagtatactc aggcttaata
     1981 gaagctggat taccttcaat tatggcaggt catatccata tgccagctta ttcaaaacat
     2041 tttaatcctg atctgaaaga tgaagattta cttcctgcaa ctttatcaaa agaattaatc
     2101 actgacttac ttagaggaaa actcggattt aatggattag tagttactga tgctagtcat
     2161 atgttaggaa tgactggggc aatgaaacga tcagaattat tgcccacttc tattgcagct
     2221 ggatgtgatc tatttctatt ttttaatgat ccagatgaag actttaatta tatgatggaa
     2281 ggctatcgta aaggaattat cactgatgat cggttattag atgccttaaa aagaatttta
     2341 gggttaaaag ctaaattggg attacacaaa aaagcaaaag aacaaatttt attacctaag
     2401 caagaagctc tggaaaaaat tggtttaatt gaaaataaat caatttttaa agaggtggct
     2461 gaccaagcaa ttacattagt taaagataaa caagatattt ggcctattgt accagaaaaa
     2521 tataaaagga ttttactagt agatgtacat ggggtaaaag gtgggtttgg tgaagtagtt
     2581 gctggaaata gacctcaagc cattgattta ttgaaacaga ttttgtcagc aaaaggattt
     2641 gaagttgaaa aatacatttc acctgaagaa gaattgaaag gattatcgca agaagaaaaa
     2701 attaaaaata tagctggaat atatgctgct aagagaccaa ttaaaaattt aactaaaaaa
     2761 tatgacttga tcattaatat agctaatgtt aatccaggca cagttcaacg aattgtctgg
     2821 ccagcaagta aaggaactcc ggatataccg ttttacattc atgaaattcc aacaattttt
     2881 gtatctgtac agtgtccgtt tcatttggct gatgtacctc aagtaaagac ttatattaat
     2941 acttatgatg gaaaacaaga aactttagaa atattggtgg acaaactaat gggtctttct
     3001 gaatttaagg gagtaagtcc aatagatgct tattgtggtt taaaggatac atttatttaa
     3061 tatttagata ggagtggttt atatgttgta tcccattatg acagagacga ggcaagtaat
     3121 tgacttaagc ggtctctgga gatttaaatt agatacaaat gatgacttac aggaagaact
     3181 tgctttagaa ccaataaatg aaaaagagag ttatccgatt tcagttccct cttcttataa
     3241 tgatttattt gaaacagaga acattagaaa tcatgttgga tgggtgtggt atgaaaaaga
     3301 agtgactctt cctaaaagat tattagaaga aagattagtt cttcgttttg gatctgcaac
     3361 acatgaagca aaagtttttt tgaatggaaa gtttattact caacacaaag gtgggtttac
     3421 accatttgag tctgaaatta atcggtttgt aaaagacggg aataatcgaa ttacagttgc
     3481 tgtaaacaat attattgacg atagtacttt acctgtgggg attatgaagg aaacagtaca
     3541 ggaaaatggg gatataaaaa aggaaaactt agttaatttt gatttcttca attatgcagg
     3601 tattcataga cctgttaagt tatatacaac tccgaaagac tatataagtg atattactat
     3661 taatacaaga ataactgatt tggatgcaag tgtagaattc aatatcgata cagaatggac
     3721 cggaaaaatc ttaatagcga tatatgacga agaggctcaa cttgtagctg aagctgaagg
     3781 aaagaaagga atgattcagc ttaagaatcc aattttttgg gaacctctag acgcttattt
     3841 atatagtatg aaaattagtt taattgatga taataaagtg atcgatacct atacagaaga
     3901 atttggtatt cgcagtgttc aggttaccaa tggcaaattt ttgattaata ataaaccttt
     3961 ttattttaaa ggatttggta agcatgagga ttcgtatgtt aatggacgtg gtttgaatga
     4021 ggctgtaaat attaaagatt ttaatttaat gaaatggata ggtgctaatt cttttagaac
     4081 atcacattat ccttactcag aagaaattat gcgattagca gatcgagaag gtattgtagt
     4141 tattgatgaa gtacctgcag tagggttaca tttaaatttc atggccactt tattagatga
     4201 taatgtggaa aaacataata cgtggaaaga gatacgaaca gaaaattctc ataaaacggt
     4261 gatcaaggaa ttgatagaac gcgacaagaa ccacccaagt gttgtaatgt ggtctattgc
     4321 aaatgaacca gattcggatt ccgaaggtgc taaagaatat tttgaacctt tagttgaatt
     4381 aactaaagaa cttgatccgc agaaaagacc tgtaacaatc gttacttatc taaaatcaac
     4441 gccagatgtt tgtaaggtag gagatattgt cgatgtttta tgtttaaatc gttattatgg
     4501 ttggtatgtt tcgggagggg atctggcaca tgcaaaatat ctgttgaaaa aagagctaga
     4561 tggatggatg gaaagatgtc ctgatacgcc aataatgttt acagaatatg gagcagatac
     4621 agtagctgga tttcatgata cagtaccaac aatgtttaca gaagagtatc aagtggctta
     4681 ttacgaagca aatcacgaag taatagatac atgtgaaaat tttgttggtg aacaagtgtg
     4741 gaattttgca gattttgcaa caagtcaagg gattttaaga gtgcaaggaa ataagaaagg
     4801 aatctttact agagatagaa aacctaaaat gattgcttac tatctacaga aacgatggtc
     4861 atcaataccc aatttcagtt ataaaaaata atttggattt tttaagaatg ttcaaaaaac
//
LOCUS       DACDRN010000118         4807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDRN010000118 DACDRN010000000
VERSION     DACDRN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.118"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            69..152
                     /locus_tag="IWJ80_002683"
     CDS             69..152
                     /locus_tag="IWJ80_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP9462344.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            277..1014
                     /gene="erm(B)"
                     /locus_tag="IWJ80_002684"
     CDS             277..1014
                     /gene="erm(B)"
                     /locus_tag="IWJ80_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9462345.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1184..1444
                     /locus_tag="IWJ80_002685"
                     /pseudo
     CDS             1184..1444
                     /locus_tag="IWJ80_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1607..2272
                     /locus_tag="IWJ80_002686"
                     /pseudo
     CDS             1607..2272
                     /locus_tag="IWJ80_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2308..2553
                     /locus_tag="IWJ80_002687"
     CDS             2308..2553
                     /locus_tag="IWJ80_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9462346.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2656..3525
                     /locus_tag="IWJ80_002688"
     CDS             2656..3525
                     /locus_tag="IWJ80_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP9462347.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3506..4240
                     /locus_tag="IWJ80_002689"
     CDS             3506..4240
                     /locus_tag="IWJ80_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9462348.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4273..>4807
                     /locus_tag="IWJ80_002690"
     CDS             4273..>4807
                     /locus_tag="IWJ80_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HAP9462349.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAID"
BASE COUNT         1721 a          697 c          938 g         1451 t
ORIGIN      
        1 ttgtgtataa taggaattga agttaaatta gatgctaaaa atttgtaatt aagaaggagg
       61 gattcgtcat gttggtattc caaatgcgta atgtagataa aacatctact gttttgaaac
      121 agactaaaaa cagtgattac gcagataaat aaatacgtta gattaattcc taccagtgac
      181 taatcttatg actttttaaa cagataacta aaattacaaa caaatcgttt aacttctgta
      241 tttatttaca gatgtaataa cttcaggagt gattacatga acaaaaatat aaaatattct
      301 caaaactttt taacgagtga aaaagtactc aaccaaataa taaaacaatt gaatttaaaa
      361 gaaaccgata ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct
      421 aaaataagta aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca
      481 gaaaaattaa aactgaacat tcgtgtcact ttaattcacc aagatattct acagtttcaa
      541 ttccctaaca aacagaggta taaaattgtt gggagtattc cttaccattt aagcacacaa
      601 attattaaaa aagtggtttt tgaaagccat gcgtctgaca tctatctgat tgttgaagaa
      661 ggattctaca agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa
      721 gtctcgattc agcaattgct taagctgcca gcggaatgct ttcatcctaa accaaaagta
      781 aacagtgtct taataaaact tacccgccat accacagatg ttccagataa atattggaag
      841 ctatatacgt actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa
      901 aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cgttacttat
      961 gagcaagtat tgtctatttt taatagttat ctattattta acgggaggaa ataattctat
     1021 gagtcgcttt tttaaatttg gaaagttaca cgttactaaa gggaatggag ataaattatt
     1081 agatatacta ctgacagctt ccaagaagct aaagaggtcc ctagcgccta cggggaattt
     1141 gtatcgataa ggggtacaaa ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc
     1201 taagtgtggc aacaatattg tattaaaaaa atcgttttat ggttgttcaa attatcctga
     1261 atgtaagttt actttagctg aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa
     1321 agaattactg gagggaaaag aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc
     1381 ctacaatgcc gttgtaaaaa ttggggaaaa gggatatatt gattttatct ctttttcaaa
     1441 ataaacatgg ggctgaccca aaagtagctc ctatatgaaa aatagaggtc tttccattta
     1501 gatagtagtg gaaagacctc tatttatctt ttgaaagtga atacaaaaat aaagcacact
     1561 ctgctataat ataggtgatt aatccatttg agagaggtgc tttatcatgt atcaaaatta
     1621 taccataaac caactttgtt tgcctataga tcttgaaatt aaattagaag aaaatgattt
     1681 tgctcatgcc attgtgcagt ttgttgactc tattcccgat gaagtctttc ttccttatta
     1741 tcaatctatg ggtagacccc aatatcatcc tcgtatgcta ctttccatta ttctttgtgc
     1801 ctatattcaa ggggtttatt cgggccgcca aatacaaaat atgctgatag atagtattcg
     1861 gatgcgctac ttatctcaag agcaattccc taactttcgt actattaatc gttttcgcgt
     1921 tcatccgatt atgaacgata ttctagatca ttcatttgtt caatttcgtg agctactggt
     1981 tcaaagtggc ttaatcagtg gcagtgctct atacattgat ggtacaaaaa ttgaagcgga
     2041 tgcgaataaa tactctttcg tttggaaaaa aagtatttta aagtataagg atagccttga
     2101 tcaaaaagcc cttgaaaact accaacaaat ggtggaaaca aaaatcttac ctgagttaat
     2161 agatgattta aaggatgaac tttctataga agagattgaa aggattcgtc aatctttgga
     2221 agaaaaggaa aatcaattaa ttgaagcgat tgaccaaacc gaaacagtag aagggggctg
     2281 acccattatt aataaggagt ggacaacatg attgatgtaa atgagctatt ggaagaagct
     2341 attagagaaa ctgaaaatct aaatgatggc gaagtttttc ttgttaaaga tttattcaag
     2401 ggatatgtgg ggaataggat acccagaaag gatcgacttc tgcttggaac gttattttta
     2461 aaccatgtta gcaaaatgaa tggtaattta aaggctattg aaaagacttc atctaatcag
     2521 cagaggtata aaaagacaat tgataagttt taactatctt ttgtgaaatt aaaaaaatta
     2581 taaatatgtt gagtagaagc aaaagatttg gcttggaatt tttacagcag tgaatcgata
     2641 aaaggagcat gcttgatggt agataatatt attaaatcag tagcagagaa attatcctct
     2701 ctgtcttata tagaaggtat tgttttaggt ggttcacgtg caaggggcac ccatacagag
     2761 gattcggata tagatatcgg catctattac aattcagaat catttgacat aaatactatt
     2821 aatcaattcg ctacaaagct ggatgatgag catagaaata accttgttgt acctcccgga
     2881 gcatggggtg attggattaa tggcggcgga tggttagtca taaacgggta tcatgtggat
     2941 ttaattttac gtgatattaa acgtgtggaa caaataatga aagatacaga gcacggaatt
     3001 gttactgcca attatcagac tgggcatccc catggttata ttagtgcaat gtatcgagga
     3061 gaattagcga ttagcaaaat actatatgct aagaatgaaa gcttatgcga attaaaaaaa
     3121 caggcagaaa cttatcccaa tgctttgcag aaaagtttag ttaacttttt tatgtttgaa
     3181 gcagggttct ctttaatgtt tgtaaaagca aattcgggaa cagacgataa atattatatt
     3241 gcgggtcatg tttttcgtat agtttcatgt ttaaatcaag tgttatttgc atgtaataat
     3301 gcttattgta tcaacgaaaa gaaagctata aaactgcttg aaacttttga acataaacct
     3361 gaaaaatata ccgagaaggt aaatcatatt tttgaagtac tcggtatctc actttttgaa
     3421 tgctacgaca tgaccgagaa gctttataat gaagtgaatg aaattgtatc ggagataaat
     3481 aactttttaa acgaggagag ttcagatgaa agaaaacaaa tatgatgata atatattttt
     3541 tcaaaaatac agtcaaatga gtcgctcgca gaaaggactg gctggtgcgg gagaatggga
     3601 gactttgaaa aagatgctac ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg
     3661 ctatggatgg cactgtatat atgcgatgga aaacggtgct tcctctgtag taggtgttga
     3721 tatttctcat aaaatgctcg aagtagcaaa aggaaaaacc cattttccac agattgaata
     3781 tgaatgctgt gccatagaag atgtggattt cccagaggag agctttgatg taatactaag
     3841 ttcgcttgcg tttcattatg tagcagacta tgagaattta ataaaaaaga tatataggat
     3901 gctgaaggct ggtggcaatt tagtttttac agttgaacat cctgttttta ctgctcatgg
     3961 aacacaagac tggtattata acgaaaaagg agaaatactg catttcccgg tggacaatta
     4021 ttattatgag ggcaaacgga cagctatgtt tttggaagaa aaggttacaa aatatcatag
     4081 aacactgacc acatatctaa atacactgct ttcaaatagt tttataataa atcagattgt
     4141 ggagccacag ccgccagaga acatgatgga tattccgggg atggcggatg aaatgcgacg
     4201 cccaatgatg ctgattgtat cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg
     4261 aggagtatgt aaatgagatc agaaaaagaa atgatggatt tagtactttc tttagcagaa
     4321 caggatgaac gtattcgaat tgtgaccctt gaggggtcac gcgcaaatat taatatacct
     4381 aaagatgaat ttcaggatta tgatattaca tattttgtaa gtgatataga accgtttata
     4441 tctaatgatg actggcttaa tcaatttggg aatataataa tgatgcaaaa gccggaggat
     4501 atggaattat tcccacctga agaaaaggga ttttcctatc ttatgctatt tgatgattac
     4561 aataaaattg atcttacctt attgcccttg gaagagttag ataattacct aaagggcgat
     4621 aaattaataa aggttctaat tgataaagat tgtagaatta aaagggacat agttccgact
     4681 gatatagatt atcatgtaag aaagccaagc gcaagggagt atgatgattg ctgcaatgaa
     4741 ttttggaatg taacacctta tgttattaaa ggattgtgcc gtaaggaaat tttatttgct
     4801 attgatc
//
LOCUS       DACDRN010000119         4803 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDRN010000119 DACDRN010000000
VERSION     DACDRN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.119"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(54..1202)
                     /gene="nagA"
                     /locus_tag="IWJ80_002691"
     CDS             complement(54..1202)
                     /gene="nagA"
                     /locus_tag="IWJ80_002691"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP9462350.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1278..2114)
                     /locus_tag="IWJ80_002692"
     CDS             complement(1278..2114)
                     /locus_tag="IWJ80_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9462351.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2298..3515)
                     /locus_tag="IWJ80_002693"
     CDS             complement(2298..3515)
                     /locus_tag="IWJ80_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9462352.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3898..3981
                     /locus_tag="IWJ80_002694"
     tRNA            3898..3981
                     /locus_tag="IWJ80_002694"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3932..3934,aa:Leu,seq:gag)
     gene            complement(4221..4799)
                     /locus_tag="IWJ80_002695"
     CDS             complement(4221..4799)
                     /locus_tag="IWJ80_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462353.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1457 a         1001 c          775 g         1570 t
ORIGIN      
        1 aaaccttaaa accaaactta agaagtttag ttttaaggtt aacttaaatt atattatgcg
       61 tggaaacgtt ccacgccatc tagataagtt gcttccaatg tcatatcagg gttcaatacg
      121 ataaagtctg catcgcgtcc ttgagcgatt tttccacatg tatcatcgat tttacagctg
      181 attgctggaa tcagactagc catcatgact gcttgttctg gtgttgctaa tccccagtct
      241 acaacatttt taattgcttc tttcaatttc aatatacttc cagctaagtt gccagattct
      301 agacgtgctg ttccttcttt tactactact ggaaattcgc ctaaattgta atttccatca
      361 ggcatcccac cggccatcat acagtctgta attaatgcga catgatcatg tcctgctttt
      421 tccattagga ttcccgcagc atttggatgt acatgatggc catcacaaat caattcagaa
      481 aagacatgtt ctaaagataa taacgcaccc accatacctg gttcgcgatg attcaatcca
      541 cgcataccat tgaaagcatg gacaaataca cttgcgcctg cttcaacagc tttttgcgct
      601 tcttctagtg tagcattact gtgtcctaga gaaacaacta ctccttcttc agtcacttgt
      661 ttgacaaact cttctacacc ttgacgttcc ggtgctaaag caattttttt gattaaacca
      721 cctgaagctt cttgccattc atggaaagta tccaaatcag gatccccgaa ataacttggg
      781 ttttgtgccc ctttgtgttc ttctgtgaag aaaggacctt caaaatagat tcctttgatt
      841 ttagctcctg gtgcttcctg atacattttg ccgattgtct cagctacatc ttttaaacgt
      901 tctttgcttg acgtcaatgt tgttggtaaa aatgaagtca ctccacaaga taacagacct
      961 tcagacatca ctttgatgcc ttctgcatca ttatccatta catcatgatt catatagccg
     1021 tgaatatgtg tatctaccaa accaggagca atccatttgc cgctttggtc aatgatgttg
     1081 acttctccct caggtagttc tttggtgtat tcaccaaaaa gtccatcggt aatttcaaga
     1141 tagcctgcat gtttgacacc tgacgctaaa aagaatttgt cagcatagat aaaagttctc
     1201 attctattct ctccctttgt ttttctacct ctaaagtact cttatttaga gaagaagtca
     1261 agaatggtct acaccattta caaatttttt gaaatttgtt tcacagcttg tgctaagcct
     1321 tcatagaatg ctgctctttc tggttcttct ttacgcttgt atattaagct tttaagcaca
     1381 aataaatcat tgaggtaata gtagctattc ttctcttgag cccaatagat tccttgattc
     1441 acctgttcta acgcttcgtc gatttcattt tgatggaata agaaatttcc ataattataa
     1501 aaaatttgcg aaaattcact ttttgatcgt tcatcagctg attgatgcag cagttggatg
     1561 cttcgactca aatagtagcg tgcttcagcc gtttttcctg ccacaccata tatttttccg
     1621 atacaactaa tcagttgtat ttctgtgtca gaaacatgga ttctttcttt ttggctagag
     1681 tagcttaatg cttctttcaa ataataaagg gactgagaga tattttgacg caacgtgaat
     1741 tcgcaaacac cgagataata ataatagcgt tgaaagtcag ttgcttgata cagattttca
     1801 gaaatatcag aagattttag tacttgttct aacttccgat aatcacgttt ttggaaacaa
     1861 ctatccaaca gttcaaataa tttatcgttt gtccgagtag tttttcgaga gagattcatc
     1921 acatgatcaa tcgttacccc tagccgttga caaatctgca tcatcaccaa tacattcggc
     1981 agttcctcat tattttcgat ccgacttaat acactttgtg aacaaatatc atgcgaaagc
     2041 attttttgcg tcattttttg ttcttttcga atctctttaa tgatcgttcc aaaggactcg
     2101 attaacgttt ccataagtat cctccccaaa atatgcgtat tcgcattcta ttcgtatttt
     2161 actttaggaa gtcgcaaact tcaagaaaag acttgcaact aaataatata ctttagttat
     2221 ttaagaaaac tccactagaa agagagcctc agtgtgtttg tctgtggccc tcctactgaa
     2281 aaatttggtt tttaaaatca tttttggcga taggttgcta ggaattctct cattttctta
     2341 gctgtttgtt tcaaatcatc cattttagga agataaacga ttcgaaaatg atctggctgg
     2401 ttccaattaa atccaccacc atgtacaagt agaatatgat gttcatgtaa aaagtccaaa
     2461 acgaattttt catcatccaa aatattgaat cgctttgtat caatttttgg gaatatataa
     2521 aaagcagctt ttggttttac ggctgaaaga ccaggaatat cattgattgc attatagata
     2581 tattctcttt gttcatagat ccttccgcct ggcaggagca gctcatcgac actttgataa
     2641 ccgcctagcg cggtttggat aatttgctga gacaatacat tggaacacaa acgcatggag
     2701 gacaacatat tcagcccttc tatatagcct ttgacgtgtg acttatctcc acttaggacc
     2761 atccagccta ctcgaaatcc tgctacacga tgagatttgg atagaccgtt cagcgtaaca
     2821 acgaacaaat caggcgctaa tgtagcaatc ggaatatgtt ctagaccatc catcactaag
     2881 cgatcataga tttcgtcgga ataaataatc aggttatttt gtcgagctac ttcaacgatt
     2941 tcttctaata attcttttgg ataaagtgcc ccagtaggat tattaggatt aatgataaca
     3001 atagcttttg tccgactcgt tatttttgat ttgatatcat caatatcagg ataccattct
     3061 gcctcttcat cgcaaatata atgaacaggt gtgcctccag ctaatgcaat agaagccgtc
     3121 cacaacggat agtccggcat tggaaccagt acttcatctc cattgtccag caacccctgc
     3181 atacacatcg tgatcaattc gctcactccg ttgccggtgt agatatcatt gatcgtcaca
     3241 ttcggaaaat gtttcagttg gcaatattgc tcaattgctt ttctagcaga aaagatcccc
     3301 ttggaatctg agtagccttc tgagtcacgg gcattcatga tcaaatcacg aatgacttcg
     3361 tttggtgctt caaatccaaa aggtgcggga tttcccgtgt tcaattttaa aatactaatg
     3421 ccctcttctt gcatccgctc cgcttcttct agaacgggac cgcgaacatc gtaactgaca
     3481 ccttctaatt tgttggattt ttcaaattct ctcatcactt agccaatccc tcatttcaaa
     3541 aagttagaac tttccaaatt ttttgttatt tactatttca atatcatact ccatcctaga
     3601 aaattttgcc acttatttag gaaaaacgta agtattaaaa aaacacaaac aagcgttgag
     3661 aacttaattg tcatgatagt cattccatag attcgctgcc caatccattg caattccaca
     3721 aatgattcct agcataaaac caagcaaaaa acgtccgaat gcaagtccaa tcaatacacc
     3781 taagatcatt cccagtcctg aaaattctcc gtcatatttc tccatcttaa taagtcctcc
     3841 catttttgag cttgacaact atgtaaaacc tcgctatcat tagtgtgtta ttattacgca
     3901 gatgtgttgg aattggcaga caagcatgat tgagggtcat gtgagcgtta gctcgtgtgg
     3961 gttcgagtcc catcatctgc actacaactc gtgaagttaa ttttgcttca cgagtttttt
     4021 tgtttgaacg tttctttatg ccattatata catatcactg tatcctttac aaaaagaacg
     4081 tacaatcatg taaacagcct gaggtgagta tatgaaggtc ggaaacaagc tactcgtaga
     4141 aacttgcaag tgttaaaaca attaatcgaa aaacgagtaa aatccctcac ctaatactaa
     4201 agcgagggat tttttgtata ttatctgttt atcaccgtta ttataaaggt acaaatacca
     4261 agcacggtta atccaaattg cataaattca tttttttgta cactcatgct tttcattagc
     4321 tctttaactg ccattagtac taagatggcg ataacaacat aaagtatata gtacgtccgc
     4381 attccggaag cccatactgt gggagaaaat cccataatta accttgaaca aaatcccatt
     4441 atcatcaaaa taactaaaaa aatagaagtt ttcttattgt caaaacttaa ataaattcca
     4501 acaattaaac atagtaataa tgccaagact actaaagtag ctatccaagt acctggataa
     4561 aaaagtgaca gtttcgttcc taactctgta aagagattat tcaggttact actactccaa
     4621 atcattgccc ttttatttcc attgacgtaa gtaaagcttt gacccattgt attaccaaaa
     4681 taaataatca ggtttaagaa aaacggtaat gcagtcaata tacgcacata atagttttga
     4741 catttcctat atgttagaag gaaaattaag aaaaatagca ataaaaataa gatattcatg
     4801 tct
//
LOCUS       DACDRN010000120         4524 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDRN010000120 DACDRN010000000
VERSION     DACDRN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.120"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            172..1068
                     /locus_tag="IWJ80_002696"
     CDS             172..1068
                     /locus_tag="IWJ80_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9462354.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            1071..1784
                     /locus_tag="IWJ80_002697"
     CDS             1071..1784
                     /locus_tag="IWJ80_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP9462355.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            1804..2322
                     /gene="msrA"
                     /locus_tag="IWJ80_002698"
     CDS             1804..2322
                     /gene="msrA"
                     /locus_tag="IWJ80_002698"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9462356.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            2319..2546
                     /locus_tag="IWJ80_002699"
     CDS             2319..2546
                     /locus_tag="IWJ80_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP9462357.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            2698..3249
                     /gene="lepB"
                     /locus_tag="IWJ80_002700"
     CDS             2698..3249
                     /gene="lepB"
                     /locus_tag="IWJ80_002700"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9462358.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(3332..3874)
                     /locus_tag="IWJ80_002701"
     CDS             complement(3332..3874)
                     /locus_tag="IWJ80_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9462359.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(4111..4509)
                     /locus_tag="IWJ80_002702"
     CDS             complement(4111..4509)
                     /locus_tag="IWJ80_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9462360.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         1628 a          791 c          811 g         1294 t
ORIGIN      
        1 attatgtaga ttagtcgaaa agaggataag ccctcagcct gaaaggcttg cagcttattc
       61 tctttttctt tataaaatta ggaaaataat tgttttcttt tttcataaag actacctttt
      121 tgataaaatt tttgataaca ttagaaccaa tcgaatacaa gaggtgaaaa aatgaaatta
      181 acgaaacgac atttgatcct cgttttgttc ccattattaa ttgcgatcat catctatgca
      241 ggtttagctg cttctatacc gaaagctggc ccactaatga aaccagtaac tactgtgaga
      301 gaagatccaa ataaaaaaga aaaacttcac tacatagcta tcggagattc gctgactgaa
      361 ggagtaggag atcaaaccaa gcaaggagga ttcgttcccc ttgtcgcaaa tgatctaaaa
      421 gaccgttata atttgaccgc ggttgaaaca gaaaactatg gtgtaaatgg tgaacgaagt
      481 gaccagatct taaagcgagt aaaaacaaaa gaagaaattc gaaacaatat caaaacagct
      541 gattttatca cactgactgt tggcggtaat gatttgatga aagttatcca aaataattta
      601 ttcgggttaa cggtcaattc ctttaaaaag ccactgaaaa actatcagga aaatgtcaca
      661 gaactattgg aagaatttcg atccctcaat aaaaccgcac ctatttacat tttaggcatc
      721 tacaatccat tttatttgaa ctttcctgag atcactgaca tgcagacaat tgttaacaat
      781 tggaacaaag gaacagaaac ggtgacaaaa aaaatgccga actgctactt tatcccaatc
      841 aatgatttgc tttatcaagg attgaatcag gaagtaggtg tcacagaaga aggaacagat
      901 caaacaacta gcacgacaga aaatctaaat ggcatcaaaa ataatgtgct ctatgaagaa
      961 gatcatttcc atccaaataa tttaggttat caattgatgg caaatgcagt gagagatcag
     1021 ctgatcgaga cacaaaacca atggttagag aaggaggatg atcaataaac atgaaaacaa
     1081 gatctgacgt atcttcaaac aaaacaaaga aaacaaatta ctggaaattc gccttcctta
     1141 tccttcttgg ggtagtactt ggttcgaccg tatttttagg cagccgtatt tttgctaatc
     1201 gggaaccaga actcccagaa ataccagctc ttacggaaag acaaggagat cctgtcttaa
     1261 cgatcaattc aaataaagaa aaagtcaatc aaatcatcag ctttttcctt agtgagtatc
     1321 aaaaagatag tgatattgaa tataaatttt atttggaaaa tgaagcacta ctcaatggta
     1381 ctttcgaagt actaggtttt cctatcgatt tctatttata ttttgatccg tatgtgatgg
     1441 aaaacggaaa tgtccaacta aaagctagaa gtctttctat cggaacattg agtttgccaa
     1501 tcaaagatgt gatgaatatg atcaagagaa actataaatt accagattgg atcgaagtca
     1561 atacagatga tctgactatc atgcttaggt tggatcaatt ccgaatgcaa aacgggatgt
     1621 atattaaagc agataaaatc gacttagtaa atgatgacat tcggtttagt ctatatttgc
     1681 caaaagatac ctctgatgct tcaaacaact caaataccaa caaaactagc accagcagta
     1741 ctgaaagcaa gacaaaagaa aataaaaaat catcggatga gtagtattag agggggaatc
     1801 ataatggaac gagcaatatt tgcaggaggc tgcttctggt gtatgatcca gcctttcgac
     1861 acgcttcctg gtatccatac agtgatgtca ggatatactg gagggcatgt accagatccg
     1921 acttatgaac aagtaaaaag ccaaacgact ggacatacag aagcagtgga gattatctat
     1981 gatccaacat tgatttcata tgaacaatta gtagaacttt actggcagca aactgatcca
     2041 actgacgctt tcggacaatt cgaggatcgc ggtgacaatt atcgcccggt gatcttttat
     2101 ttgacagaag aacagcggat cattgctgaa aaaagtaaga aacagttagc tgaaagtgga
     2161 cgtttcgaac agccaatcgt tacaaaaatc gaaccagccc aaccatttta tctagcagaa
     2221 gaagaccatc aagagtatta taaaaagcat cctgaaaatt ttaaaagaaa ccatgcacgc
     2281 cgggctgcct ttatcgaaga aaattggagg aacgaagggt gaatcgaagc ttttttcatt
     2341 atttaatgac actgcgaggc ggtggcacac atgatgcttt atctgttttc gctacagaag
     2401 ccggtaaaga catccaattt cctaagcaca gctctagcta cgaagaaatt tctaattatc
     2461 tggaactgaa tgtcgactat ttgccgtcca tggatatctt tgacgaagca tgggaaaaat
     2521 atttagaaaa taatcatgac atataagaga tagttttgta ctttatgatc aaaaaagttt
     2581 tttgtccaat agcctaattg aaagaattat ttttatttca gctatctagt cgcatttttt
     2641 tgatattttt cttataataa agaaagaatg aaaaaaagag ggatggattg atagagattg
     2701 acaaaaaaac aacgctatat taatcgcttc tggcttgtat tcaaatactt acttgtttct
     2761 gtattccttg cgttcatgtt gcggggattt ttattcatcc cggtaccagt ggaaggcaat
     2821 tcaatggaaa acgtgttgaa gcaaggtgat atggttgtga tggaaaaatt ttcggagatc
     2881 agacgatttg atattgttgt ttttcaatta gcagatggca cgatttatat caaacgagta
     2941 atcggtcttc caggagaaaa tgtcagctac caaaatgatc aattgaagat caatgggaaa
     3001 gtagttaagg aaccttatct aactaaaaac ctgaaatcag atcatgcaaa tgcatcctat
     3061 acgacggact ttacactaca agagctaacg ggacaatcca agcttccgga agataattat
     3121 tttgtacttg gagataatcg aagagtttca aaagatagtc gttcgtttgg cacgatcaat
     3181 aaaactgata ttctaggtaa agcaagattt gtctattatc cacttgatga aataaaatgg
     3241 atccagtgag aaacaataaa gcgtgcagtc tgttgagact gcacgcttta ttgtttaatc
     3301 caaaatcagt ttactacctt agtacgtaaa atcaaacagc tcgtttaaac acttttctag
     3361 cctctaaagt tttgataaat gaataaacaa ccattatttg aatagggatt tggatcatat
     3421 tcctgatgaa tctacctaga aaaattactt tgaaatctgc accaaacaaa acagacatca
     3481 gccaagaatt gataaaaagg ctgactggga tcataatcaa taattgggca gcgatgacgg
     3541 aaggcaggtt tatcttttta tgatataaaa ataagccatt aataaatcca ccaaggaaag
     3601 ccgtgataag aaaagcagct tgaaacgttc ctgaaccaaa tatcgtgaaa tttaaagtgt
     3661 tcgacaacat ttcaagcaaa ccagcccaga tcggtccata tagataaccc ataagcgcca
     3721 tacttaaaaa tccgaatgtg atttgaaaat aagcaggacc aatcgctatc ttgtataaga
     3781 tgacatcaag ggctaataaa agagcagagg tcgacaacaa tcttgcattc atttcgggca
     3841 atctaaaaac gtcttttgca atcaaagtgt tcaaaatatt ctctcctttg tcagttaata
     3901 ataaaaggat accataaaat caccaatgtg tcatgacaca ttggtgattt acatacactg
     3961 gcatctaaca attaaaaaaa gggtgagaag actgctttta taaactgtaa aaaagagatt
     4021 gtacgaataa aaaaaataga ggcggagaat acttatcgta tcatccgcct ttgttttttt
     4081 gttataaaaa acgtcagatt agttatctgc ttacatcttc atccatacgg gttctaatgt
     4141 atttccgtct agatcttgga cttctaatga aaacatttct tcttcaggca ttcccatatc
     4201 tacttcgtgg aaagtaccac cattttcttt tgcaatttga gcaaattttt ttacttcatc
     4261 cgcattttca acactaaacg aaatcaaagc accacttgtt tttttattat cagctatttc
     4321 tttatctttt ataaattttt tataaaaatc atgagagaga agcattatcc aaaaagtgtc
     4381 atcccatacc attgaaccag cttcttcagt agaaaattct tcattttttt taaaacccag
     4441 cttttcataa aaagcaacgg aacgattgac atcagcaaca ggaaaattaa caaaaaccat
     4501 actattcatt tgtttcgtct cctt
//
LOCUS       DACDRN010000121         4465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDRN010000121 DACDRN010000000
VERSION     DACDRN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.121"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            574..1008
                     /locus_tag="IWJ80_002703"
     CDS             574..1008
                     /locus_tag="IWJ80_002703"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462361.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="IWJ80_002704"
     CDS             2298..3221
                     /locus_tag="IWJ80_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9462362.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="IWJ80_002705"
     CDS             3237..3776
                     /locus_tag="IWJ80_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9462363.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1667 a          674 c          757 g         1367 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacggagaa cttaaattaa attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat aaaaaagact
      181 gctctttcga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tatgaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacacccca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttga cttagaccaa tcgaaaatca caaagtgagc ccctgtatcc cttggtagag
      421 aggctacttg gcatagtaca cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct ctcaacgtgg taaaaatccc acttataaga gaaaatggtc aagaacctaa
     4261 acggtcggcg aagaaaacat accaagcacc acaagcaaaa aaagttagag taacttccaa
     4321 aaaagaaaaa ttttttagaa caacttttga aaatttaatt ttgatttata tccgttcaat
     4381 ttaaacattt gataaactga aggctagtat ttaaaatgtg tatacaagtg gtagcgttaa
     4441 aaaaagtgga ggtataacct atgtt
//
LOCUS       DACDRN010000122         4415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDRN010000122 DACDRN010000000
VERSION     DACDRN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.122"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            58..912
                     /locus_tag="IWJ80_002706"
     CDS             58..912
                     /locus_tag="IWJ80_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9462364.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1009..3258
                     /locus_tag="IWJ80_002707"
     CDS             1009..3258
                     /locus_tag="IWJ80_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9462365.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3261..>4415
                     /locus_tag="IWJ80_002708"
     CDS             3261..>4415
                     /locus_tag="IWJ80_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9462366.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1556 a          625 c          805 g         1429 t
ORIGIN      
        1 taataatata aacataataa aacaaaaatt atccaaatct aacataggag ggtggatatg
       61 gaaaattcta tatatttagt aacagaacct aacactcagc tgaaagattg ttactttaat
      121 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
      181 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      241 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      301 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      361 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      421 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      481 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      541 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      601 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      661 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      721 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      781 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      841 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      901 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      961 tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat gtataaggat
     1021 aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt taatcaggag
     1081 ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata ttatggtgag
     1141 aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc tatgacaacc
     1201 tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga atttcctgaa
     1261 tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa tggctctcgc
     1321 ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa actagaaggt
     1381 ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt ttatatttta
     1441 gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga ttatcctgtt
     1501 attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat cgagaatatt
     1561 agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt atctggtgct
     1621 tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca atcaatcgaa
     1681 tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa aagaaaaaat
     1741 actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg aaacttctta
     1801 attcaagcag aagtagatac atgggatgtt actagactac aagttggcat caatccattt
     1861 ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc gattctggtt
     1921 tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt tagaaaacgc
     1981 ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa ttgggaagca
     2041 acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc atttgatgtt
     2101 ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa cgattgtgca
     2161 ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg cagcctttca
     2221 gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga aatggtgaat
     2281 aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc taatcgaaaa
     2341 cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt tgttgaaaat
     2401 attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat taagtgggat
     2461 atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca acaaggagaa
     2521 gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat aacaaaatat
     2581 cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc tggaatgctt
     2641 cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag gttaaaaatt
     2701 caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt atcaattgtt
     2761 ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt agcttttttt
     2821 ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt gaggattgta
     2881 aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg tactttttat
     2941 agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt tagtcatgat
     3001 aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc tccttaccgc
     3061 cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca aaatagtttg
     3121 cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc tagtggacaa
     3181 ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat ttgggttata
     3241 acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa ggagcaatct
     3301 taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt ccacaaatga
     3361 aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca ccaagtataa
     3421 ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga atgaaaaaga
     3481 caataatttt aggtttatta ttagctggta taggaggaat tattccagta tttacagcta
     3541 ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga gtatataatt
     3601 cattggctgt gacatatatc agctctttat actctggcaa tactagagca acgctattag
     3661 gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt gctggagttt
     3721 tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt cctgtagctc
     3781 tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata cctgaaaatc
     3841 atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca atcttactcg
     3901 taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa attaaagggg
     3961 taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata ctagcaggat
     4021 ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg ggaattatat
     4081 tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg ttcctgctaa
     4141 cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc tttagtaact
     4201 tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt tttattggct
     4261 gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt ctaacaagaa
     4321 gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg atcctatttg
     4381 taacattttt tgcgacaaat aaaaaaaata ctaga
//
LOCUS       DACDRN010000123         4387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDRN010000123 DACDRN010000000
VERSION     DACDRN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.123"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(3..1451)
                     /locus_tag="IWJ80_002709"
     CDS             complement(3..1451)
                     /locus_tag="IWJ80_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9462367.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(1451..2167)
                     /locus_tag="IWJ80_002710"
     CDS             complement(1451..2167)
                     /locus_tag="IWJ80_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9462368.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(2148..2498)
                     /locus_tag="IWJ80_002711"
     CDS             complement(2148..2498)
                     /locus_tag="IWJ80_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9462369.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2642..3415
                     /locus_tag="IWJ80_002712"
     CDS             2642..3415
                     /locus_tag="IWJ80_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9462370.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            3586..3952
                     /gene="ssrA"
                     /locus_tag="IWJ80_002713"
     tmRNA           3586..3952
                     /gene="ssrA"
                     /locus_tag="IWJ80_002713"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(4257..>4387)
                     /locus_tag="IWJ80_002714"
     CDS             complement(4257..>4387)
                     /locus_tag="IWJ80_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9462371.1"
                     /translation="QVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT         1297 a          855 c          781 g         1454 t
ORIGIN      
        1 ttttactctt ttttgatttc tacgtcaggc aaacgaacag cgaattttgt tccttttcct
       61 agttcgcttt ctacttctaa ttttccttta tgcaagcgta caagttgctg cacgattggt
      121 aaacctaatc ctgattcacc atattttgta ttttttcttg aaggatctac tttataataa
      181 cgatcccaga tacttttcat ttgttgttca gacattccaa ttcccgtatc ttcaatcgtg
      241 atgatcgttt caagatagcc tttttctaaa gtaatcgtga tttggccgtt ttcagtaaac
      301 tgtatcgcgt tttgaataat attgaccatg atttggacaa atcggtcata atcagcgtaa
      361 acatcaatat catcaacggc tttcaatgtc aatgtatctc ctgctgcttc tgctttagct
      421 tctaactgtg caacaatatt tttcaatgtt tctgttccat tgaatttttt aatgaccata
      481 ctgatttgat tcgtacggat tttttcataa tccaagtttt cattcaccaa acggatcaga
      541 cgctctgttt catttttcat caatttaatc gcgttttctc gctgattttc agggattgca
      601 ttgtattcta acccttccag aagtccgttg attgtcgtaa gaggtgttct catctcgtga
      661 gaagcatctg ccataaattg tcttcgacga ttttcttgtt cttcaatttc tgcttgagat
      721 tctttcaatg aattcgtcat tttattgaaa tcatctgcta actcatcaaa ttcatctttg
      781 tcatgtacgg ggagctgtac atcaaaatta ccattcgtca cttctttcgt cgcattcctt
      841 aaccgattga tacgcttaac ttggaatgct gcaaaagcat agctggcaat aactgcaacg
      901 atactggaaa aaataaatcc tttgaacagg ttcagtgtaa ttggacgaac gctgttatcg
      961 atatttcgcg caggttgact aactacaagt gctccataga attcatgatt caaattaaat
     1021 ggcacaagtg cgtaagaggt cgcctgtgct tgtcctaaaa tatttttatt cgatgtgaat
     1081 ttttcttgac gtccattcct cagatttttc cattgatcag ctgttaaggt aaaattcagt
     1141 aaagctgtat tagtcggata aatgacacgt tcgtttttat cgataaatat aaagttcaca
     1201 ttttgttctg tcagtacttg ctctgtcagc agcaatgcat tttgaaacgc ctgatcttga
     1261 ctaaactgcg ggagcgtatc ggcataggtt tgcgttgtct tttcaactga ttcagcatat
     1321 ccaaacagtt gctggtaatt gttttcttca atcgtttgtt ttgtcagttg tgtaaaagaa
     1381 actccaacga tcaataagat caacgcgacc attccgcaaa aagctaagag ttgctgatac
     1441 aaataacgca ttaggcaaca ccagaatcat cgaatttgta gccgactccc caaacagttt
     1501 ggatgacttg tggtccgacc ttttcgattt tttgtcgtaa tttcttgata tgggcgtcca
     1561 ctgtccgttc atcaccaaag tactgataat cccagactaa ttcaagcaac tgctcccgag
     1621 agaatacttg acgtggtttt tttgctaaag taaacaatag atcgaactct ttaggcgtca
     1681 gtccttcaat tggccggttg tccagatacg cttctcgtgt tttcgtattc atcttgaaat
     1741 gactagtctc gatatcaaaa tgatcatctg aaactgctgc ttcttctctt gcttctgcca
     1801 actcactacg gcgatgcaaa gctttcattc gagcaataag cgtcagagga ctgaaaggtt
     1861 ttgtgacata atcatctgcc cccatctcta agccgatcac ctgatcgctt tcagaatctc
     1921 tagctgtcag catgataatc ggcacagtac ttgaaacttt gcggatctct cttcctacta
     1981 gcataccgtc cagtgtgggc aaattcaggt ctagtgtaat catatcccaa tgttgtgggt
     2041 tttctaaaaa ggcatcgagc ccttcttttc catcatactt gaaagttgct tcccaacctt
     2101 catttaagaa aaacatctgc atcatttcag aaacagattg attatcttca atcattaaaa
     2161 tattcattat cgttccctcc agtttccagg tcatatttct gtaatttctt ttctgtcata
     2221 ataaaaatag gcataattgg aaatactagc agaagcattt caatcagaaa ataattcgta
     2281 tgcccagatg tctttaaata atagccgatc aatgccatca gtaagcccat aagaaaaaac
     2341 cagtttccgc ttgtcttttg agccagccgc caatgagcgt ctgtttttgc ggcaagaggc
     2401 gaatgatacc cataaaaagg cagatttcct tttgagggca gaatccgaaa aagactgcca
     2461 attattaaga ataagatacc aatactgaaa aaaatcattc tttccctcct aaattctctt
     2521 cttatagtat actctaaatt gttgactgaa ccaatatgtt caggaattct tcacgaaagg
     2581 tttgtctttt gttcattctt tgttaagata aacttattcc aataaaaaag gagttttatt
     2641 tgtgtcaaat tatcgtgcaa tcactttctt tgatcttgat ggaacattac tagatgcgaa
     2701 atcaaaaatc acacccgaag tggcaaaagc tatgaacgca ttaaaagaaa acaatgttct
     2761 ccccgtcatc gctacaggac gtacagaagc agaaatccac tctatcatgg aagatgcggg
     2821 cattacttct gcggtcacca tgaatggttc tttcattcga gtagacggcg aagaaatcta
     2881 ttctcaaaca ttttcttcag aagaatgtca aaaaatgtat gatcatgtac agttgaacgg
     2941 tcatgaactt tcttactata acgatcagaa aatctggtgt accggtcata gccaaacaat
     3001 gatcaatgct tacgacttca ttcattcaga tgttccggaa attgatcctt taggctttaa
     3061 agaaaacaca gtaaatatgt tgctgatctt atctcaatca ggtgacgaat actatcacga
     3121 acattttcct gagctaactt tttatcggaa tggtccttac tcgatcgata cagtgaagaa
     3181 aggaatctca aaaggggcag gtgtcaaacg tctgcaaaaa gaacttcagc tggaaaaagt
     3241 tcctactttt ggatttggcg atggtccgaa tgattttgcc ttgttggaag cttgcgacaa
     3301 caaaatcgct atggacaacg cctatgacgg attaaaagaa ctttctacct ttatcacaaa
     3361 gaaaaataca gaaggcggaa ttgtccacgc attgaagtat tttgatctga tttaagatct
     3421 ttttttgaag ctacctcttt tcttaagaaa atcgcttatt ttttcttaag aaaggggcag
     3481 ttattatata aataatggga tatgattcgc ttttttattg aatttatccc aatatatgct
     3541 atactaattt tgtatgaaaa atacacgtaa cccttgttag ctaacgggga cgttacggat
     3601 tcgacaggca cagtcgagct tgaattgcgt ttcgtaggtt acgtctacgt aaaaacgtta
     3661 cagttaaata taactgctaa aaacgaaaac aactcttacg ctttagctgc ctaaaaacag
     3721 ttagcgtaga tcctctcggc atcgcccatg tgctcgagta agggtcctaa ctttagtggg
     3781 atacgtttca actttccgtc tgtaagttga aaaagagaac atcagactag cgatacagaa
     3841 tgcctgtcac tcggcaagct gtaaagtgaa tccttaaatg agttgactat gaacgtagat
     3901 ttttaagtgg cgatgtgttt ggacgcgggt tcgactcccg ccgtctccat atatgtatgg
     3961 ttgagacaga taaacatgta tgaaaaacgt tgttaaatca acgttttttt attttggtca
     4021 aggagagaaa agtagagaaa tacaaaattt tttgcattga ttttgcattc aaaaaaacat
     4081 tcaaacattt agaaatcccc aatacgtatt caggaaaatc tattttaagg tttacaaaaa
     4141 gaacttttgt tcgtacactc tttttgaggt gaacttttat gctgatggaa ggaaaaacac
     4201 aattatggtt taagcatgtt ccttcaaata agttttttta attattattt gtcaaattaa
     4261 gcgagacaaa actttccatt tacgtaactc aaaaaaactt ctaatggtgt ttggtaattt
     4321 agtgattttc tagggatatg gtttcttttg gacgcaacgg atgagatgaa tccttgattg
     4381 acttggt
//
LOCUS       DACDRN010000124         4080 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDRN010000124 DACDRN010000000
VERSION     DACDRN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.124"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..143)
                     /locus_tag="IWJ80_002715"
     CDS             complement(<1..143)
                     /locus_tag="IWJ80_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9462372.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTI"
     gene            complement(465..686)
                     /locus_tag="IWJ80_002716"
     CDS             complement(465..686)
                     /locus_tag="IWJ80_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462373.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(784..1029)
                     /locus_tag="IWJ80_002717"
     CDS             complement(784..1029)
                     /locus_tag="IWJ80_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462374.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1269..1883
                     /locus_tag="IWJ80_002718"
                     /pseudo
     CDS             1269..1883
                     /locus_tag="IWJ80_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2253..3254)
                     /locus_tag="IWJ80_002719"
     CDS             complement(2253..3254)
                     /locus_tag="IWJ80_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP9462375.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3413..3679)
                     /locus_tag="IWJ80_002720"
     CDS             complement(3413..3679)
                     /locus_tag="IWJ80_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9462376.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3669..4025)
                     /locus_tag="IWJ80_002721"
     CDS             complement(3669..4025)
                     /locus_tag="IWJ80_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9462377.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1290 a          740 c          541 g         1509 t
ORIGIN      
        1 taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg aacttcacga
       61 tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc ttttttgaat
      121 tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc aattttacac
      181 taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata cttcttccca
      241 gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt ggtttgccca
      301 tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat ttcaattata
      361 tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa gctgtatttt
      421 acatatagta accaaagagt taataaaata aaaagagcga accattatag atcagctctt
      481 ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa ggtatttaca
      541 tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc aatattacgg
      601 taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa ttcccagcgt
      661 ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac ctcaaactta
      721 aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa atatgaaaaa
      781 aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct taatgtgttt
      841 cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg ttgtgtattt
      901 tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat agttcatagg
      961 agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta caacttctgg
     1021 aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt tatctttcct
     1081 tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta tttaactttt
     1141 agaattcaaa aaccgtttgt ctatttagag tttaccctaa atagacaaaa atataacatt
     1201 gaaaaattta gagttctaac gtatgattag ggtgtattat aaacgttata gaaaagtagg
     1261 gaataaattt gaaaattgct tatattagag tttcatccat cgatcaaaat gaacaacgtc
     1321 aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt tatcgaaaaa caatctggag
     1381 cgacgattag tcatcggccg attttccagg aagctttaga ttttgtcaga gagcaagata
     1441 tttttattgt agaagccatt gatcgacttg gacgcaatta tgacgagatt atcgattcag
     1501 tgaattatct aaagaagaag aatgtgcgac ttattatcac cagtttaccg atcatggctg
     1561 aagcaattgg aaatcctctc ctagataaat tcatcaaaga cttaatcatt taaatctcag
     1621 ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg ccaggcacaa ggaattaaaa
     1681 tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt atacagtgca gacaccaaag
     1741 atcctcaacg tcgccttgtc tataggagta tcgttcagga tcttgagaat ggggttgcta
     1801 tctcaaagat tgcaacagac tacaatgtga ctaggcaaac aatttaccga ataaaaaaag
     1861 aaatcgacca actaattgta tgatttcttt tttgaatcac gtaattatta attagctttg
     1921 tcatcaatac agacaatcat tttagattca tctagctaag ttgattacat ttatgttaaa
     1981 aagaatttag ttaacataaa tataattgat gcataaatcg tccttattta aaggcattta
     2041 acattgacaa cttagtataa tgtatcttat gtaaactaat aagatttgaa ttttatttta
     2101 tacacactcg ataatagatc aattttatgg tcccccgcat ttacgattgc acaagagaaa
     2161 cataaagtcg acctattagc atttcttttc aattcaaaca ttaaaaaagt ttatacctct
     2221 gctactaaaa aatgaaatta tctatttaaa atttaaaacg gtaaatctga ttcatccaaa
     2281 ttggaaaatg gatcagcctg tattggagct ttgtatttct ccataataac agatatcttt
     2341 ttttcatttt ttaaaaaata ccttttaaat ttcttatagt cctctgaaat caaaatatag
     2401 cgtgatggat actcatcaaa agaagaatcg tattcttcct ttaaaattcc cgatttactt
     2461 aatttttcta gtacatcaaa tactctatca ctcaagtaac tccttaaatt gttatcactt
     2521 tcccattgaa ctacttttct ataagttgaa ttatcagttg aaaagataga attttcatcc
     2581 ataaacgcat atattagcag caatctttca tcattgtcaa attctattgg atcaaaaata
     2641 tctaagttgt catcaaaatc tttatcagtt ccggtaactt ttttgttaat tactgaaggt
     2701 gtcttgtaag aaatagaatc taaatcaaag cgttttgatt ctaaattatt ttgtgctttc
     2761 tcaattaaat aattcacttt ttttgtgaga aaccttctga tattgtcttt gctatatgtt
     2821 ccataaaaac ccttatcttc taaagagtcc ctgtacttct taacttctat atactcttca
     2881 gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc ctacctcgcc tttgctattt
     2941 ttataaaagt aatcaatttc tgctatagtt cccgatctat aattatcaac tggagaaccc
     3001 aacttatggc caaaaatcgc taataatata tcactatttt ctacaatttg tttattaata
     3061 acactttgcc cagagctatt atttatataa gtaggaatcg cattcgtttc ccatcgaatt
     3121 ggcatcaaaa ttacttttgt attatctgta ttttctatat tccatctata tatggtttct
     3181 tctacttcat tcctctttgc tgctatatca cctggtgatg ctataaatac ttttattaga
     3241 tttatggttt tcatttattg tacctttctc tttatgtctc tttaaagtaa tttttgtata
     3301 aaatttattg acttttacac tataggacaa cttttatcta tttagtacaa aaaggcccac
     3361 taaactaatt gattagttag gagcctatta caggtatatt ttcaactcgc ttttaagcaa
     3421 attctactac cagctttttg cctaatgccc tagcgatatc atcaagttta tcgaaagata
     3481 cgttatcccc tctctcaatt cgagcaatag tagattgagt tgtatgtgct ttttgtgcaa
     3541 gctccgcttg agttaggcct gcttcctccc gtgcatgata aagagcaacc gcagtttcga
     3601 gttttcttcc ttcacggtca tatgcttctg caaaatcttt gtctttttgc ttttcttcaa
     3661 tcaatttttt aatcatcatt tgtgaaccac tcctctctaa gttcttttgc atgtttaagt
     3721 tcacttattg gtgttttctc cgttttttta gtaaatccat gggtaatgac ataccttcca
     3781 ttgaccacat ggaaatagat agctctttgg atgtttgatc ctaccttact tcttaattcg
     3841 tacaaattag tgtctaattt tttcacccat ttcaaccgtt gagctactag catcccattc
     3901 ttttctgttt cttctattgt acgtaaaagc ttcgcactat ctttttttgg taatgtttcc
     3961 aaccattcta aaaattcatc atgaccatta ggtcttttaa atgtttcaaa agatattttt
     4021 tccataccca attatatcat atattgattt tatattatag tatataacat aaaaattcga
//
LOCUS       DACDRN010000125         4006 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDRN010000125 DACDRN010000000
VERSION     DACDRN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.125"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            110..481
                     /locus_tag="IWJ80_002722"
     CDS             110..481
                     /locus_tag="IWJ80_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9462378.1"
                     /translation="MTVMRLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPE
                     TASSIRTIELDDNTITFLKAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIV
                     GVHIIRIHGLRHSHASLLISQ"
     gene            complement(765..1850)
                     /gene="xerS"
                     /locus_tag="IWJ80_002723"
     CDS             complement(765..1850)
                     /gene="xerS"
                     /locus_tag="IWJ80_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP9462379.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            2184..2417
                     /locus_tag="IWJ80_002724"
     CDS             2184..2417
                     /locus_tag="IWJ80_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP9462380.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            2414..2821
                     /locus_tag="IWJ80_002725"
     CDS             2414..2821
                     /locus_tag="IWJ80_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9462381.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(3272..3781)
                     /locus_tag="IWJ80_002726"
     CDS             complement(3272..3781)
                     /locus_tag="IWJ80_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462382.1"
                     /translation="MKKVLFAVSLVVNIALAGGLFYTITEMNKLDDQRESLAISNEQY
                     KRKLDEASSTNVDTQEAVESSTIVYSNESSETLTNSERSTAPATETVSQKATITIGSK
                     VFTFIVNGLTIEHDSSRTGKMTELSHEFPVIVTTPSATLNGILVQDNNDNNCYIMVDG
                     QTLYRGTYQ"
     gene            3897..>4006
                     /locus_tag="IWJ80_002727"
     CDS             3897..>4006
                     /locus_tag="IWJ80_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9462383.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT         1326 a          618 c          669 g         1393 t
ORIGIN      
        1 aactacagtt gatttttgga caaaagaaga atttacaaaa gttatttcaa ctttttatat
       61 tgatgatttg taccaacatt ttggttttat ttgtatgtgg ttacttttta tgaccgttat
      121 gcgtttggga gaagccactg cattaacttg gaaagatatt gattttgaaa ataaaaactt
      181 gcgtgtgaat aaaacactct attataagag agccaaaacc tatgagtttg tagaacccga
      241 aacggcttct agtattcgta caatcgaact agacgacaat acaattacct ttttaaaagc
      301 ttggaaggag cgtcaagttt caataggagg catgtcgttt gtactttctt ataatggaac
      361 acctactcag aaatatacaa tccgtcatat tatgagaaga catgcaacga tcgttggtgt
      421 tcatataatc cgcatacacg gattaaggca tagtcatgct tctttgctta tatcacaata
      481 aaaagatgca cttcgaatta aagaaaggtt aaaacatgct gatgtacaaa cgactttagg
      541 aacttatggt catttgtatc taaattcatt gagagagata acaaatgatc ccacagatta
      601 tatccaaact aatttttcta aaatcaaacc gacacctatt tacgataata aaaaataaat
      661 gataaacaaa agtaggatca caccaaaaga aacacactag aaaaactata atacaggctt
      721 ttctagcgtg ttgtttctta tttccgccta tttatacatt atactcataa agcatttaat
      781 gcatcgatcg tagccgcatt gtctatatgg gtatataaag ttgttgtatt ggtacttgaa
      841 tgtcccaatt gttgggcagt taataccaat gaatttgtct gttggtagag tctagtagct
      901 aatgtatgac gcagtttgtg agggctgact tgaattctaa agccttttga atattttgct
      961 accatccgtt cgattgcaat accggatata cgcttggctt ctccttggta aattgctaga
     1021 aataaagctt tttcgttttc tgttacttta tatcgttctt tccgaatagc tagataatta
     1081 gctaaatatt ccattgcgaa tgctgctata ttgacagcat ctcttttatt tccttttcta
     1141 gttactacca cattattctt atctaaagat aggtcatcta aattaatatt tataacctcg
     1201 gagagtctca atccagatcc taaaatcagt gcattgatcg ccaggtctct ttctacatct
     1261 cgttgataat agatcaaagc tcgtttactc aaagtatttt tatattcatt ggcaatataa
     1321 tctagataat tttgtgtatc cttatctaga aataattttt tctcaatggc attggcacgt
     1381 gcagctaacg tagttttatc tgtgatggtt gctacttgat ccataggatt attgtctaag
     1441 tatggttttc ctagaggatt attggaactt ttcgaaagat atttaaatag aacctttaat
     1501 gccgtgatat ttctattgat agtagaagta gatagaccta agttattatt atctttgagc
     1561 ttagtttttg ttgtctcttt ttttacattt tcttttgtgc gacgtttcaa atgagatttg
     1621 tacaagttta ggtcataggc agttaactgt cttatcgagg tgatagaagt atcaggaacg
     1681 atagactcac tcagaagcca ttcataaaag agcttataat tttttagata ttctaaagtg
     1741 gttcgaggtg attgatcttt atcctcacga tcttgaacaa aatcttttat ataatcgggc
     1801 atatgaagta tgattgtatc tattttttga tatatttctg tatttttcat gtcattcatc
     1861 ctttcaataa gtaggggaga agagtatctc tataattgga ctataccaat tatagaatgt
     1921 tttgtcaatt ggtatgctct aaaatttatt aatttgtttc aaaaaaagca tataaacagg
     1981 tatacgttta ataatttaaa ataaatggta tacataaata gggaaggaaa tagaagggag
     2041 caatctaaat tttttgaatt ttcttaatta attatataga aagaacaatc tttctataga
     2101 gttagacatg agagggacta tgcttgattt aaaatcaaca agcgtatata ctgtatatac
     2161 aaagaaaaag gagggagaag actatggcaa ttaaagaaag aaaatttagg gaagtgggga
     2221 actcagtagt attaactatt tcaaaggagt ttcttaaaga aaatgggtta aatcccggag
     2281 atacggtttt tttagatgaa gaaaaacttc gagcggcaat tacaaaaaaa caagccttat
     2341 ctgatgaaat tgatttagca atatctcaag cattaactga atatgatact acttttagag
     2401 agcttgtcga taaatgagta ttcattacct agaagccaaa gatattataa aaatgaatgt
     2461 aatccaaata aaaaaatact ctcctaatga accccttggt gtaaaggatg cgaatgcttt
     2521 agaaatgtgt gtagggcaac ttcgagcctc agcttttggt gaagaggttt atccttcgat
     2581 ttatgaaaaa gcagctatcc tacttataca attgataaag aaacatcctt ttcacaatgc
     2641 aaataaacgt actgctttct tagctacttt tgtctttttg aaaatcaatg gacagctttt
     2701 agctattgat caaaaagatg ctgtaaattt agcggtatat attgctacgt atgataagga
     2761 tttcgatcaa ctaaaatatg aagtgatcaa cactataaag acacatacgc agcccattta
     2821 aggagttact gtatgtgtct ttatagggtt attagctgct atagcgaaat aattgaagta
     2881 aattacaaat agctataatt caaatccaac tatatagaaa acgtaaattt tctatatact
     2941 tggatatgct ggaatgttta agagaataat tacatttcta gaaaatacaa taaaaattac
     3001 aggataatta gccatatcct acctcctcta gcagttgctc aaaagttagc actaagtaac
     3061 ttcttttttg ttgctggtaa caatttatca atactaaaat agcgtttaag tatctaataa
     3121 atactaattt taaagctctt aattattatg aaacacgata taacaatatt gctatatcgt
     3181 gctattgtaa tattatacat ttgaagacag cgtattcttg ttctaatatc aggaattttt
     3241 tataaatatc agtcatatta agactcaaaa tttattggta tgttcctctg tatagagttt
     3301 gaccgtctac cattatatag caattattat cattgttgtc ttgcaccaat ataccgttaa
     3361 gggtagcact aggagtagta acaatcactg gaaactcatg agaaagctcg gtcatttttc
     3421 ctgttctgct agaatcgtgt tctattgtca agccatttac tataaacgtg aatactttac
     3481 ttcctattgt tatagtagct ttttgagaaa cagtttctgt agctggagct gtgcttcttt
     3541 ccgaatttgt tagggtttca ctggattcat tgctataaac aattgtagag gattctacgg
     3601 cttcttgagt gtccacgttg gtgctgctag cttcatctag cttccttttg tattgttcgt
     3661 tactgattgc taagctttcc cgttggtcat caagcttatt catttctgta atggtataaa
     3721 ataatcctcc tgctagtgct atattaacaa ctaaactaac cgcaaataat acctttttca
     3781 ttcttccttc tcttctttct ctttttgtaa ttccttactt ttggataggc tacaattaac
     3841 tagacagaaa aattaaggtg tgtagactag aagaaaacat accaggagga atttttatgt
     3901 ctaagagaac acgaagaact ttttcacaag aattcaagca acaaatcgtc aatctttact
     3961 tagctggaaa gccacgtgta gaaatcattc gagaatatga actaac
//
LOCUS       DACDRN010000126         3817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDRN010000126 DACDRN010000000
VERSION     DACDRN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.126"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(77..367)
                     /locus_tag="IWJ80_002728"
     CDS             complement(77..367)
                     /locus_tag="IWJ80_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9462384.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(503..682)
                     /locus_tag="IWJ80_002729"
     CDS             complement(503..682)
                     /locus_tag="IWJ80_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462385.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(679..1029)
                     /locus_tag="IWJ80_002730"
     CDS             complement(679..1029)
                     /locus_tag="IWJ80_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462386.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(1022..2347)
                     /locus_tag="IWJ80_002731"
     CDS             complement(1022..2347)
                     /locus_tag="IWJ80_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9462387.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            2797..3411
                     /locus_tag="IWJ80_002732"
     CDS             2797..3411
                     /locus_tag="IWJ80_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9462388.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(3576..>3817)
                     /locus_tag="IWJ80_002733"
     CDS             complement(3576..>3817)
                     /locus_tag="IWJ80_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462389.1"
                     /translation="SFMYRDFYEESIELTPTEPNKSQITLSKELERKVESQELAIGIS
                     ITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT         1177 a          732 c          600 g         1308 t
ORIGIN      
        1 gcattttctt agaaaaaaac aatattacga tatcatgata ttaagatatc gtaatattat
       61 acaattgtga ttgatattat atatgttttt ttattttttt atcatgttta atcttgaagg
      121 aatcatattc tttttctaaa gacaggagct tttcataaac atcagacatg tttaaactag
      181 aggccaggct taaatcataa acgaatgaag caggtaatga ttttattcta cgttgtcggg
      241 ttatccagct tgcaaccgtt ccttgtgggt aggcatataa ctcacaaaat tgttctacag
      301 tgattcctaa attcttgata atgtaaacat tgatcgggtg cgagtaaaca aatgtattga
      361 tagacatggt gcgtaagcct actttctttt attattgcgt ttgcaataat agtttaacac
      421 acgcattatt ctagtatcta ctcctatttg taaaaacaaa aaaggattca tagagataac
      481 tctatagaat ccgtaaaata ccttatatat ataacgggaa gatataaaac ctaaaattat
      541 gataacctgc tgcagcttct acatagaaag agaagcgctc aacaccaacc tcatttatca
      601 aacgttggcc ataaccccaa atctcttctg atagctgatc aaaggttata tactttagct
      661 catattgctg gataatgttc attctaatcg actccattta ttatcctggt aaaattcgat
      721 atgacgtatt tcgtcgtagt gaattttgtc ttttccaacg tagatcccca gctcatcact
      781 acctaagatc ttgccaatga tatcttctgg atagtatcct tcagcgtttc gttcttcttt
      841 ttgaatagat actgatctat tttttatgac tgcagcgttc aatacttccg caatctcttc
      901 agtcgtcatt tgttcttttg ctaaattttg actggttcgt tgttcattgt cggcatcaat
      961 ttcttcagta tgatcagata aataaaaccc tgcccacttt aaacgtccac gatcattgta
     1021 atcaatcatt tttaattccc ccatttccgc ccgcatgacc accaacaagt gtgcttctag
     1081 taatgcttct agcgccttct aacattgaag aagcatgtac aatcgacgta aaaccatatt
     1141 tttgccgaat cttgtcaata attttatcta aattttcttc gtcaatttgc ttttgtggat
     1201 cactaaataa atccagctga agcgaatcgg tgtacacaag cttgctatag gttattccaa
     1261 catgccttac ttcttgtcct tcataatact tatcaaacaa gaaaagacaa taattctgca
     1321 attcctttgt attatctgtg atagggatct tcatctggtg tgaaaaccct ggtctcgttt
     1381 ctaagatcga cgtgccaatg tgtaaatgga cacaacctgt tttacagtta tgctgcctaa
     1441 tgcgtatcgc tacttgttcc gccatttctt ttaccaccag ctcgatttca tttcttctag
     1501 tataatctcg tggtaacact tgtgaatttc catatgattt ttcctttgtc ggttctggtg
     1561 ggattgctat atctgttcga tcgatcccat tagcatggaa gtacagctgt aagccaatca
     1621 cacctagtct attcttaatc gtataaggat tccagttagc aagatctcta atgctcataa
     1681 tccccatctg attgagtctt tttttcattc gagagccaat tccccaaaag tccgtcattt
     1741 caggaatatt ccatactttt tcaggaacgt tttcgtacgt ccattccgca acaaatcctt
     1801 cattatgctt tgcttcgttg tctaaggcta gtttagccaa aaggggatta tctccgacac
     1861 ctactgtagc aatcaatcct agctcttctt taatattctc ctggatcatt tgagccagct
     1921 tctttctccg ttgacttcgt gttccttcag tcgtaaaaag attcagtgat cgggtcactt
     1981 ttaagattga ttcatcgatc gagtaaatca gtagatcttc atcagccaca tatcttctga
     2041 aaatattatt tacctgcata tttcgcttga tatataggtt catacgtggt ggaacaacgt
     2101 gtagtgtttt aggaaatggt tgtggtaagt cacgtggtct actcacattt gtaataccat
     2161 accgcttttt tgcttcagga gaagaagcta atatcaatcc tgaaccagta ttatcagctc
     2221 gactcataac aacaagttct gttgttaatg gatctaaatt tctttctata cattcgacac
     2281 ttgcgtaaaa gcttttcata tcgattaaaa agtaatcatt tactggctct tttgaataat
     2341 ccatcataaa taacactcca ttctttcaca ttatacaaac atatgttcga tattgtaaag
     2401 aagaaatagc taaatacata aaaaaagaag cataggtatg ctatcaccct gcttcttctt
     2461 ttgatttgta tgatcaattt attctatcac tttactaatt aatttgaaaa tctaataaaa
     2521 actctcactc taaaagagtg agagactgca aattagagta cggacttttt tggagttccg
     2581 ttctttaata ttaacaaatg aataaaatcc ttacaacatt tgagaataag ttatcagtag
     2641 acacttacaa aaaattctca tactatttcc tccatcatta gtttttgtct atataaggtt
     2701 taccctaaat agacactggt ataacattga aaaatatggc ttcctcgtgt atgattaggg
     2761 tgtattttaa atagttgcag aaagtaggta aaagttttga gagttgcata catacgtgtc
     2821 tcatcgatcg atcaaaatga acaacgccag attgaagaaa tgaaaaaatt cggtgcagag
     2881 agaattttca tcgagaaaca atctggcgca acgattactc atcgacctgt tttccaagaa
     2941 gcattagatt acgtccgaga tcaagatatt tttattgtgg aagctattga tcgacttgga
     3001 agaaattatg acgagatcat cgcttcagtc aattacttga agaagaaaaa tgtgaagctg
     3061 atcattacta gtttaccgat catggctgaa gcaattggca atcctctcct ggataaattc
     3121 attaaggatc tgatcatcca aattttggcc atgatcgctg aacaagaacg gactgaatcg
     3181 aaacgtcgcc aggcacaagg aattaagatc gcaaaagcaa acggcgtcta caaagggcgg
     3241 cccaaattat acagtgcaga cgcaaaagat ccccaacgtc gccttgttta caaaagcatc
     3301 gttgaagatc ttaaaaatgg tgttgcgatt gctaagattg ctaaagacta caatgttacg
     3361 agacaaaccg tgtatagaat aaaaaaagac agcatggtca atgacaaata gttgatcatg
     3421 ctgcttttat tatcttctca tctttaattt cctacatata attactatct ataaaattaa
     3481 gtcttactca gaacagataa agcataaaat tccaacaaac cattttttac ttaactaaat
     3541 aaagagtctg ttaatataat cttcacttat ttacgttaat cccctactgt atttgttggt
     3601 tccttatcta tattttctac aaaatcctta ttatcttgtg atttagcttc gtataattct
     3661 ttgtatgtcg acacattggt tatactaatt cctatagcta gttcttgaga ctctactttt
     3721 ctctctagct cctttgacaa agtgatttga gatttatttg gttccgttgg agtcaattct
     3781 atagattctt cataaaaatc tctatacata aagctat
//
LOCUS       DACDRN010000127         3734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDRN010000127 DACDRN010000000
VERSION     DACDRN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.127"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(268..1566)
                     /gene="eno"
                     /locus_tag="IWJ80_002734"
     CDS             complement(268..1566)
                     /gene="eno"
                     /locus_tag="IWJ80_002734"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP9462390.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1682..2437)
                     /locus_tag="IWJ80_002735"
     CDS             complement(1682..2437)
                     /locus_tag="IWJ80_002735"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP9462391.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2541..3731)
                     /locus_tag="IWJ80_002736"
     CDS             complement(2541..3731)
                     /locus_tag="IWJ80_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP9462392.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
BASE COUNT          944 a          782 c          700 g         1308 t
ORIGIN      
        1 tatttatacg agaggacttg cccatagatt cctattctac tgttctatcc ttaaaagctc
       61 ttatatgatg ctagttattg ttagctttcc tttttcaagg atgatctttt cttgtaccat
      121 tctttgtaaa catcatatac agaagctagt tgcaaaaaaa ctctctacct gctgatattt
      181 cagcaagtag agagttcgat tacttaacta agtgatcata ctcgactatc tttcaatagt
      241 cgatcatgta tcttcattag aaaagaatta tttgtttttt aagttgtaga aagatttcaa
      301 acctttgtat tctgcaactt caccaagttg gtcttcgata cgtagtaatt ggttgtattt
      361 agcaatacgg tcagtacgtg atagagaacc agttttgatt tggccagcgt ttgttgcaac
      421 agcgatatca gagattgttg aatcttctgt ttcaccagaa cggtgagata caactgctgt
      481 gtagccagct tctttagcca tttcgatagc ttcgaaagtt tctgttaatg taccgatttg
      541 gttcactttg ataaggattg agttagcaat tcctttttcg ataccttcag ataatttttg
      601 agtgtttgtt acgaacaagt cgtcaccaac taattgaact ttgtcgccta atacgtcagt
      661 taatttcttg aatccgtccc agtcgttttc gtctaatcca tcttcgattg agatgattgg
      721 atatttagaa actaattctt cgtagaattt gatcatttcg tcagttgttt tttcgccttc
      781 gcctgagtca gctagtacgt aaacaccttt ttctttgtcg tagaattctg aagaagcagc
      841 atccatagca agaacaacgt ctttaccagg tacatagcca gctttttcga ttgcttcgat
      901 gattacttca aaaccttctt cgtttgaacc aaggtttgga gcaaatccac cttcgtcacc
      961 aacagaagtt gctaatccgc gagctttcaa gattgaagct aatgcgtgga atacttcagc
     1021 acccatacgt agagcttctt tgaatgttgg agcgccaaca ggcatgatca tgaattcttg
     1081 gaagtcgatt gagttatcag catgtgatcc gccgttgatg atgttcatca ttggtgttgg
     1141 caatactttt gtgttgaatc cgcctaggta gtggtataaa ggtacttcta ggtagtcagc
     1201 agctgcacgt gcaacagcga tagaaacacc taaaatagcg ttcgcaccta atttaccttt
     1261 gttaggagtt ccgtctaaag cgatcatagc tttgtcgata gccatttggt cacgaacatc
     1321 gtagccaatg attgcttcag caatgatgtt gtttacgttg tcgacagctt tagttacacc
     1381 tttaccgcca taacgagctt tgtctccgtc acgtaattca actgcttcgt attcaccagt
     1441 tgaagcacct gaaggaacca ttccgcggcc aaaagctccg ctttctgtgt atacttctac
     1501 ttcgattgtt gggttaccac gtgagtctaa gatctcgcga gcgtaaacat cagtaataat
     1561 tgacatgttt tgtctctcct ttgagtttgt tttttcctaa gggatttccc tcatatacga
     1621 ttttagttaa ttttgctcat tagtgcaaac atttcttgca tttttcgcaa attttcaaca
     1681 attattttac agcgtctaac aatgataaga atgaatctgc ttgcaagctt gcgccaccga
     1741 ctaaagcacc gtcaacattt tcttttgcca tgtattcagc aatattttct ggttttacag
     1801 aaccgccgta ttggatacga actgcttctg atacttcttt tccataaagt ttttcaactg
     1861 tttgacgtac aactccacaa atttcgtcag cgatttgtgc gtcagctgat ttaccagtac
     1921 cgattgccca gattggttca taagcaataa ccatgtttga cacctgttca gctgaaagat
     1981 cagctaaacc agctgtaatt tgtccttcga tccattcagc agttttgcct gcttcatagg
     2041 tttctaaaga ttcgccgcag cataggattg gtgtcatacc atttgcaaag atagctttcg
     2101 cttttttatt gatgtcttcg tctgtttcat ggaaatattc acgacgttct gaatgtccga
     2161 tgatcacata atgaacacct agatcagcaa gagctgctgg tgaagtttca ccagtgaacg
     2221 cacctgagtt ttcccagtag cagttttgtg ctgagatttt tagttctgta ccttcagctg
     2281 atgtcattaa ttcttgtaag aataatgcag gagatccgat gactgaatca actttgtcat
     2341 ttgaagggat tttgtttttt actgcttcag caaattcttt tgcttctgat gcagttttgt
     2401 tcattttcca gttacctgcg ataataggtt tacgcattga ggaacacact ccttaattta
     2461 ttgaaaaatg gctagggagt catgaaaact cccttcacct tttgttcgat tactcgtcag
     2521 aattgagcaa acttggtcgt ttatttatcg ttgattgctg ctagtcctgg taattcttta
     2581 ccttcaagca attccaagct tgcgccgcca cctgttgaga tgtgtgtgaa tttgtcagca
     2641 aatcctaatt gttctgctgc tgcggctgag tctccgccac cgatgattgt tgttgcgcct
     2701 tctaagttag cgattgcttc acaaacaccg attgtacctt tagcaaagtt actcatttca
     2761 aatacaccca taggtccgtt ccaaacaact gtttttgcac cttgtaatgt atcagcaaat
     2821 aattttactg atgctgggcc gatgtctaaa gccatcaaac catcaggaat gtctccttcg
     2881 attacttgtg tttcaacatc atttgaaaat tctggtgcac agacattgtc gattggcaag
     2941 acaagtttgt cgcctgcttt ttcgattaat tcttttgcta gttccacttt atcagcttct
     3001 actagagagt taccgatctt gattcctttt gcttcgtaga atgtgtaagt cattccgcca
     3061 ccgatcaaga ttttatctgc tttaggaatc aagttttcga ttacgccgat tttatcagaa
     3121 acttttgcgc caccaaggat tgcaatcatt ggtcgttttg gttcttctac tgcttcaccg
     3181 atgaacttga tttctttttc catcaagaat ccagcaactg ttgggatacc agttgaagcg
     3241 atacctacgt tagaagcatg tgcacggtga gctgtaccga aagcatcgtt aacaaataca
     3301 tcgcctaatg aagcccagta tttacctagt tctgtatcat ttttgctttc ttttttaccg
     3361 tcaatatctt caaatcgtgt attttcgaat acgacaacgt cgccgtcatt catgttgcgg
     3421 attgcttctt ctaattgttc tccgcgagtt tcaggtacga atgttacttc tttacctaat
     3481 aattcgccta gacgttttgc aactggagct aaagattttc cttctttatc ttcttctgtt
     3541 tttacacgtc caaggtgaga gaaaaggatt gcttttccgc cttgttctag aacgtagttg
     3601 attgttggca atgctgcttt gatacgagtg tcgtcagtga tcacgccgtc tttcaaagga
     3661 acgttgaagt caacacggac aagaactttt ttgtctttta aatcgatatc tttcactgtt
     3721 ttcttagcca tttt
//
LOCUS       DACDRN010000128         3719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDRN010000128 DACDRN010000000
VERSION     DACDRN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.128"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(76..1170)
                     /locus_tag="IWJ80_002737"
     CDS             complement(76..1170)
                     /locus_tag="IWJ80_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9462393.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="IWJ80_002738"
     CDS             1359..2120
                     /locus_tag="IWJ80_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9462394.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IWJ80_002739"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IWJ80_002739"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9462395.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2895..3650
                     /gene="fabI"
                     /locus_tag="IWJ80_002740"
     CDS             2895..3650
                     /gene="fabI"
                     /locus_tag="IWJ80_002740"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP9462396.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         1191 a          726 c          655 g         1147 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatcaaagt atcatattcc taaatattta gcaatctttt
     2761 tttaatttaa gaaaaaatag tttgacaatc aaaacatttt gactaataat taagaaatca
     2821 agaagttatt ttagctatta ttctctattt ttttacgact atcaaatttt tatcagaaac
     2881 aaaggagagt cattatgtca tttctagaag gaaaaaaaat cgtcattatg ggcgtcgcta
     2941 acaaaaaaag cattgcttgg ggttgtgctg aagcaatgtt gaaacaaggg gcagaaatta
     3001 tctatactta ccaaaatgaa cgaatgaaaa aatcattagt caaattggtc ggcgaagaag
     3061 cattcactgt agaatgtgat gtttctagcg acgaaagcat cgaaaaagca atgaatgcca
     3121 tcggtgaata tgctggggag atccacggcc ttgtccatgc agttgcttac gccaacaaag
     3181 aagaattgtc cggaaatgtg tctgatattt ccagagacgg ctatcaatta gctcaagata
     3241 tcagcagcta ctctttaatt gctgtatcaa aatacgcaca accgtttctt gcaccaaaca
     3301 gcgggatcgt tagcatgact tatctaggtt cagaacgcgc aatcccgaat tataacatga
     3361 tgggtattgc gaaagcttct cttgaagctg ctatccgtta tttggcagct gagttctcgc
     3421 ctaaaggaat tcgggtcaat ggaatctcag ctggggcaat caaaacatta gctgtcacag
     3481 gtgttaaaga ctatcaaaaa ctgatccagt tatctgaatc tcgtacgcct gatcagcaag
     3541 gtgtgacgat cgaagaagtc ggtaatgctt gtgctttctt aatcagccca ctttccagcg
     3601 gaatcatcgg tgatattatc tacgttgaca aaggtgtcca tctttcataa acttttgttg
     3661 cattctttaa agtaaaacat ataaaaatca ggaaaaaact ttttaaagtc tcttcctga
//
LOCUS       DACDRN010000129         3587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDRN010000129 DACDRN010000000
VERSION     DACDRN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.130"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            338..1378
                     /locus_tag="IWJ80_002741"
     CDS             338..1378
                     /locus_tag="IWJ80_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP9462397.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2202..2534
                     /locus_tag="IWJ80_002742"
     CDS             2202..2534
                     /locus_tag="IWJ80_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9462398.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3153..3494
                     /locus_tag="IWJ80_002743"
     CDS             3153..3494
                     /locus_tag="IWJ80_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9462399.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1324 a          473 c          668 g         1122 t
ORIGIN      
        1 agtatatatt actagtaatg gtagtatgtt ttatatgcat aaggtagttc ttaatttatt
       61 ttttgttttg acactggtag tacttaatta tctttaattt taaaactaaa ttttattcat
      121 acgtttttct ggtagtattt aattataaat aatatttatt taaagtagtt cttaatgact
      181 ttaaaggtag tacataattc attttatttc tgattttagg tagtatttaa ttaaaaaaca
      241 gttttaatgc gtttgcattt tttatttatc tttgctattt tcgaaaagag taggtaattt
      301 tacataaata aaacaacgtg aaagtagagg atctacaatg actgatttta aattttttaa
      361 ggcggataga gtttacaacg aattatttta tcaatttcca aaagtcttta ttgtttctga
      421 cgaatacaaa aaaatgaaag attcaactaa gattgcctat atgcttttaa aagcaagatt
      481 agagatcgca atcagcaaac ggcaaatcga tgaagaaggt aatgtttatt ttacttatac
      541 gacaaatgaa ctatgtagag tattaaactg ccaaaaacaa aaagcgatag caatcaaaaa
      601 agagttggaa tcctttggtt tattattaca aaagcagatg ggatttaaca aacagttagg
      661 gaaaaataat cctaatagac tatatctagc agaattaaaa gtctcagaaa atgatatcta
      721 cttactcgaa aaatttgata gagagaatag ggaaaacgtt gataaatcag agggtatgaa
      781 aatcataccc accctcgacg aaaaatcaga cgctgaatcc cttggggctc aagagggtat
      841 gaaaatcata ccgtgccaaa acgttgataa atcagagggt atgaaaatca taccagaact
      901 taataataat atattagaca ctaatagaca caatatagac actgaaaaag accgcctaca
      961 agatcaattg ttgttagaca attttgagac aattatgaca aacgacagca ttgctacgtt
     1021 tgtccctgaa cgagtattaa atttgataaa aacattttct tcaagttaca gtgaagctca
     1081 aaaaaccgtc cagactattc ataatgcaaa gaaaaaagct gaaatagaaa gtgatatttc
     1141 gatagttttt gaagaactcg atagttatta tgtcaatgca gaacaagaat tatacacgac
     1201 actgttaaaa gcctatcaaa aattaaaaac cgaaaaagtc gaaaatatcc agaacctgat
     1261 ttttgtctat gtaaaaaatt ggtttatcga aaaaccaata gctgctaaag tatcaagtga
     1321 aaaacgtttg aattatgcaa gctccccaag cactattacg aaagactggt tagagtgaaa
     1381 ctttactgac aaagtgattg ttgcttcatg gagtaacatt aaaattaaaa attttttatt
     1441 attctaatta tattacgaat attacaaaat aatagctatt ttaatagaat ttataagtta
     1501 aaatattatg ataaaaatca caaacgtagc cttttacgtc ataagtgtta aaagaaataa
     1561 ttaatttcaa atatagaaaa gggaacgtat gtttgcatac tccttttttt tgagttataa
     1621 taatacgcga agagagataa tttcattatt taaacaagct aaaaatcttg atattacttc
     1681 ttttcaggca atacgaatat tattcgctta cattttttgt ataaatttaa ttgtctgcta
     1741 tgcttgtgat gaattgattt tcgttttgca cttttggatg ataaatttgg acaattgaaa
     1801 atacaaaaaa aagaaaggcg gtatgaactc ggaaggctgg taaccttcgt cagcgttcat
     1861 acacggcatt aagatttggc ccttaatgcg ttaacctttc agtgttacgg tattacccgc
     1921 aatggattta gtataacaga tattcaaaag tgataccact ttttttggct gtgttaaggt
     1981 taaaaatacg tgatattacg atgaatctat gcctaagcat aatgttataa aaatgtccgt
     2041 gtggctcttt gccgtatgac gaacgctgaa attagaggga acgcatgttt ggatgcgttc
     2101 cctctttttt gtttgtcatg aacgaaaatc aattctctat ttttgaaaaa taacaagaaa
     2161 aacttgttaa aaatatttaa agaaattggg tggtttattt tatgtcatat acaggaattt
     2221 tgagtctcaa agatatttgt cactacggta aacggtgtac cgctacagag aaaatcacga
     2281 agaaactatc aactggccaa agtaaaaccg ttgtgcagtg taagaaatat ataatccaga
     2341 aagacaaagt ctcagaggaa atgatttatt acattgggaa acaaaagcaa ataattttaa
     2401 aagatcctat ccccttaaaa gagctatatc caacaatcaa acatgtttat gaccaaaacg
     2461 gtgtactgat tggtcgaaga aaaaatggag tcttgcgttg taccgcaaaa gggatggggc
     2521 gtttgatcgg ctgaattgat cattctcata acttgtattt tatcgttttt cgcttgcttg
     2581 aaaatattgt cgagacttct aaaggaactt agagtcacta gaaaaataag gagtagactt
     2641 gatctatcaa caattccctt cggggagtga cggggtaggt tttaacgatt ggtaacgtaa
     2701 aaaattggtc aatcgagtaa atgcgcttgt ttggatagtg gtgaaatgaa tttgtgtaaa
     2761 taataagcgc agagtaaaca agagggaatt tagaagttag acaagttaga atagcgtcta
     2821 gattaatcat gagcagctac taagggatct agactttgac gcaaactcat ggaataagga
     2881 gtaggtcttc tcgtaatggg gaggacgcac aagaaattgt gtcgtgtgct gcagtacatg
     2941 tagatacttg cgaggacgtt gattattgga tcaatcgagc atttaccgct gtctactagg
     3001 gtttctcgtt tcggacgcca taaacgagtc gaaatttttt atgtattttt aagtaaacga
     3061 aaaacgataa aatatgaaca agtcctgaat aaagtgaaaa aattaatata aagcatagag
     3121 aaaatgaaat ttacgtaatg gaggaatgac aaatgaaaaa ttatgatccg aatatacgat
     3181 tgggcacaca tactattaag gtttctttcc aacgttggga ttacaaagga tttgtgacgt
     3241 ttagaagggg cggaaattgt aaaggattag atgttttagc attagacgag gatgatttat
     3301 acgatcaaac attgactgat aatccaattg gattcggctt gctacctgaa gatgatgaag
     3361 gtaatgaatg gtttaaaatg acgcttatga atgataatgg agacgaatta tctgttgaag
     3421 atacttggtc atatttgagt gattatattg tcagctttga aatcattgaa tttgtagcgg
     3481 ataaagaaga ataaaaactg gaggaataaa ttttacagat aaaggcttag aaggagtcac
     3541 gacgattaat aaaaaggtag tattacctta ggcattgagt aaggaat
//
LOCUS       DACDRN010000130         3487 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDRN010000130 DACDRN010000000
VERSION     DACDRN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.132"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            7..624
                     /locus_tag="IWJ80_002744"
     CDS             7..624
                     /locus_tag="IWJ80_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462400.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="IWJ80_002745"
     CDS             1644..2813
                     /locus_tag="IWJ80_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9462401.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3286..>3487
                     /locus_tag="IWJ80_002746"
     CDS             3286..>3487
                     /locus_tag="IWJ80_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP9462402.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHK"
BASE COUNT         1305 a          450 c          550 g         1182 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacagaatgg gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct
     2881 tgcgtaaagt tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg
     2941 ttattcatat gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa
     3001 acttaaattt catataattt aattatgtaa attagctatt ctaagaactt ctacggataa
     3061 ctgcccgtct aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt
     3121 tgtacaaaaa agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc
     3181 ccttgatttt attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat
     3241 atacataatc attaactcag aatattttta agaagggaaa caatagtgag ctataatgta
     3301 caaccgttaa ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt
     3361 aatccggaaa gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca
     3421 gatattatag gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa
     3481 cataaaa
//
LOCUS       DACDRN010000131         3398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDRN010000131 DACDRN010000000
VERSION     DACDRN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.133"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..276
                     /locus_tag="IWJ80_002747"
     CDS             <1..276
                     /locus_tag="IWJ80_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAP9462403.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="IWJ80_002748"
     CDS             419..640
                     /locus_tag="IWJ80_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9462404.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="IWJ80_002749"
     CDS             656..1498
                     /locus_tag="IWJ80_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462405.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="IWJ80_002750"
     CDS             1561..3363
                     /locus_tag="IWJ80_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAP9462406.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
BASE COUNT         1229 a          469 c          702 g          998 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaata
//
LOCUS       DACDRN010000132         3351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDRN010000132 DACDRN010000000
VERSION     DACDRN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.134"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(551..2164)
                     /locus_tag="IWJ80_002751"
     CDS             complement(551..2164)
                     /locus_tag="IWJ80_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP9462407.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2305..3213)
                     /locus_tag="IWJ80_002752"
     CDS             complement(2305..3213)
                     /locus_tag="IWJ80_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9462408.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1093 a          568 c          581 g         1109 t
ORIGIN      
        1 taaataaata ggaacaaaaa aattaaatta tttaatacac acattaggga tcattcattg
       61 aaggattaga agacaacgag gatggcgaaa aatatgatta caatagaaaa gtcgttctaa
      121 accaatcagt tcctagtatc acacaacgca agccagtcga tttggtgaga ttagaggagt
      181 gaaagcaaac tttattatat aagaaaaaat attcgaaagg gatgctagtt taggtactag
      241 tgctgataga ttaatatgat gcctcgtaat attaggcgta gttactctag agacatcact
      301 atggactatg atttgtctca gagtcacaac atgttttttt atgaaaagaa acagctaaaa
      361 taattaaatt gcgcaatgtt taaactttct taaactataa cctaataaat gtgaagaaat
      421 aatgcacaat ttcttcacat ttatcttcta ttctatatac ataccaacca acaaccctat
      481 atactttttc atttttactc ctcaagtaaa aataactcct agaccgcggc tacccaccgc
      541 ggtcgttttt ttattcttca atcaaaatcg ctttgatacc caaacctgcc aagattcggg
      601 cagaagtata agtattcaat cctcttctga aagtaatgta aactttttta tcttttggga
      661 tttcattgat tcgatctcgt aactcattca ttggaatatt ttttgttgcc gagatactgc
      721 cagaaggtgc tttattaggt tcacgtatat ctaaaaagaa cgcttctttc aaatcgtttt
      781 cgggtatatc acttgctttg atcgtttcta cgatattcgt catttgattg attgcgacat
      841 aaccagctat attcaacggg tcacgggtag tagaatacgg tggagagtaa ggcaactcca
      901 aatcaggcaa atcaaagact gtcaagtttc cggtgattgc tacagaaagt tctcccatcc
      961 gtttatcgac acctttttct ccaaatgctt gtccacctaa aatacgaccg ctatctgcat
     1021 caaaaatcag ctttagattt aatcttgtag cttctggata gaaataggca tggtcaaagg
     1081 gagtgataaa aacagtttta taatttgtta tgcctgatgc ttttaattca tgttctgtca
     1141 ttcccgcata acttgctgta tgatcaaaga tttttgcgac acctgcacca atatatccgc
     1201 gatagcgcat aggcgtacca tttaaaatat ctgccagcat atgtccttga cgattggccg
     1261 cactggaaag catactttgg atcggctgac cagtcactac actagtcgtt tcgataatat
     1321 cgccaatcgc atagatgtcc ggcagattgg tttgtaactg gtcatcaaca atgatctgac
     1381 ctgtatctga taattgtatg ccggttgctt tcaccacttc attcttcgga gaaacaccaa
     1441 cagcaaaaat tagcatatca gcagaaagga cggagccatc tgataatact atttctcgac
     1501 ctttatcacg gatctcagta actcttgttt ctaaatgaac ggctaatcct tctttcagca
     1561 atttgtcata aactaaatta gcaatttctg gatcataagg gaaagcgact tgtggcaatt
     1621 gatcgattaa agtcacgtct aagccgcgat gcttgaaatt ctcagctaat tccagaccca
     1681 taacacctgc accaagaatg acgacatgtt gtggattttt ttcttcaagg aacgatttga
     1741 ttcgatccgc atccgtcaca ctacggagga caaagccgtt ttctgcttca aaaactccgg
     1801 gaatatccgg ataatctggt ctagcaccag aagagatgat caacttatcg taatctgttt
     1861 ttatttcttc atttgtggat aggtctttaa ctgttaatcg cttagtagaa ggatcgattg
     1921 ctgttacttc gtgtttagta aagacatcga tgttgttttt tgttttcaat atttctggtg
     1981 tacgttcgat cagcgagtca cggtcagtga tcacacctcc taaataataa ggcaaagcac
     2041 aacttgcata agaaatattt tctccgcgtt catagataat gatttcgtgc gcttcattca
     2101 atcgacggaa acgagtggca aatgaaggac cgccagctac accgccaatg acaacaactt
     2161 tcataaaaac atctcctttt ctacgttgat cgtactatgt tatggtaaaa aacagcaaat
     2221 aattgtgccg gaaaattatt gtttattttg aatagacaga aaatatagag aaataatttg
     2281 tggataaaac ctaaaaattt ttttctattt taaggataga taatactttg aaggggataa
     2341 ccctgtatat tttttaaaag ctgaagaaaa atgatggatc gaatgaaatc ctaatatatc
     2401 tgcaatttct gtataggtta attcattttc tagcacgaga cgttttgctt tttccatttt
     2461 tagttcttta aaatagctaa taacactttt tccattcact tttctaaaat gtttttttaa
     2521 gctggctgga ctgataaaaa aaatttgggc taattgttca agcaaaaaat tttctgaaag
     2581 atgttcttct aaatatcgta tgattggatc aactaattca tctgaataaa gaatatgctt
     2641 ttttttagga atgactttct catttgaagg tattgtcaat aaagcaagtt cctgttgata
     2701 atgccttggt aaggaagaca attgaatttt ttgcagacaa taagtaacta ataaggaaag
     2761 caaatgaatg ctcatctgtt ggcatagtat gaaaaaagaa ttgatttcct gatcatagga
     2821 aatagctggc ttactatgtt ttagataatc aattttgaac tgttcatttt tcagaaaagt
     2881 aatgatagat gttatttctt gatctataga aaaagattgt cccgtaatat gtgctaataa
     2941 ttgctcatca aaataaaact ggataatgat aaaagtttgt gtttcctttt gagtagaaaa
     3001 gatttggact tcttgctgtt tatcaatgaa taaaagggaa ggattatcta gttctataga
     3061 tttatgtgca aattcagcat ataaattagc gctttcattt attagtagtc gaaaataggg
     3121 agcagcagat aaatgaagat gggtatctat ggaataggtt agtgtttttt tgacaagatt
     3181 ggaggattgg ttgtttgtta acaaaagaac cactttcctt tctcttttta tataaaaagc
     3241 tttctattta taattaatga ctattataac aaaaatgaaa agccaaaacg acaagtaaaa
     3301 tattttttta tataaatatt tgtgttacta attccgttaa tatacataag t
//
LOCUS       DACDRN010000133         3348 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDRN010000133 DACDRN010000000
VERSION     DACDRN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.135"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(354..1406)
                     /locus_tag="IWJ80_002753"
     CDS             complement(354..1406)
                     /locus_tag="IWJ80_002753"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9462409.1"
                     /translation="MKKIKIMHLLQSNRFSGAENIVCQIMSLFQNNEKFEMIYVSPDG
                     PIANILNIRGLSYLPLKKFSRKEIDRAVHELNPDIIHAHDFNASVRASRYKKQIILSH
                     LHNNPLWLPKLDIRTIIYSLCSSRFNSVIGVSNSVKEEFRYKQLIKDKFVLLPNTVNT
                     ERIIKMGNEFVEDDIDILYVGRMAEPKNPIGAINVLENVVKKYSKHLRVVMIGEGPLF
                     DEVKMQIQKRNLTDVIELKGFKSNPYKYMKKSKVLLMPSLWEGFGLVSVEAMLLETPV
                     VAYNVGGLKDIIDETNGFLCGSEVELSNSLMKLLNSEELRKKMGENAAISAKKFSNMN
                     RYQGVLEKIYVSAALK"
     gene            complement(1429..2574)
                     /locus_tag="IWJ80_002754"
     CDS             complement(1429..2574)
                     /locus_tag="IWJ80_002754"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9462410.1"
                     /translation="MKLLYILNVANKVNNFSKASMMAAKQTGLEFYIAGNWKYKSDEE
                     RKADEVKYGIKIYQVDFYRNPLNLKNIKAYHQIVEIIKNEKIDIIHCNTPIGGVVGRI
                     AGLKCKVKKVIYQAHGFHFYSGAPKINWFIYYPMERWLAHYTDVLITINHEDYTLAQK
                     LRLRKGGKAYYIPGVGIDTNRFMSSTSIRNFRESICKELNIPDTAKIILSVGELNENK
                     NHLSVIHALQKIPLHTDIYYIIAGVGEMRETLQNIANELGLHQRVFLLGYRSDIPKLL
                     AAANIYILPSLREGLNASLMEAMASGLPCIVGNIRGNVDLIEDGKGGFLINPKDEEEI
                     AGAINRLADNKLLCESMGAHNLDKIKDFDLSVVINQINNVYHEIVSK"
     gene            complement(2577..3188)
                     /locus_tag="IWJ80_002755"
     CDS             complement(2577..3188)
                     /locus_tag="IWJ80_002755"
                     /inference="COORDINATES: protein motif:HMM:NF012792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9462411.1"
                     /translation="MVIEEIKSSDKELINSIVDIHMRTFTNFFLTFMGKKFLELMYRS
                     YTEYNDSGILVAIQNDKPIGFLAYSGDMSGLYKYMIKKRIIPFGFYSCVAFIKKPNIL
                     MRLFRAFLKPSETKRDENYVELASIGVNPKEKSIGVGSMLIDNLKSRIDFSKYEYIAL
                     ETDALNNDVVNNFYKKNEFILERKYSTNEGRIMNEYRFYMEKN"
BASE COUNT         1123 a          601 c          366 g         1258 t
ORIGIN      
        1 aataaaaata aaataatcaa gtatgaacga tttaatgttc cttctataat aatatataac
       61 atcaaaatta ttattattag attatgccac gtcaatggta agctagaata actagttcct
      121 aatatagata tagttgatgt tggaatgaaa agaattgatg ttaacaataa gctatctgct
      181 aaacaaaaat acttttttaa gcaccacata taatacaaaa acataaaaaa agaaacaact
      241 gcaattgcta ttgagggcat cagtgaataa aaaatcattc ctgattgaaa caaaatacta
      301 atgggtatta tttttcgtaa aactaattga tctctatttt tataattcat attttatttt
      361 aaagctgcag atacataaat tttttctaat actccttgat aacgattcat attagaaaat
      421 ttctttgcac taatagcagc attttctccc attttttttc taagttcttc tgaatttaat
      481 agcttcatta gtgaattaga taattcaact tcactaccac acagaaatcc atttgtctca
      541 tcgataatat ccttcaatcc tcctacgtta taagctacaa caggcgtctc taacaacatt
      601 gcctctactg atactaggcc aaacccttcc caaagagatg gcattaatag aactttagat
      661 ttcttcatgt acttataggg attacttttg aatcccttta actcaataac atctgttaag
      721 tttctttttt gtatttgcat cttaacttca tcaaataatg gtccttcacc aatcatcacg
      781 acacgtaaat gttttgaata tttttttact acattttcta aaacattaat tgcacctatt
      841 ggatttttag gttctgccat gcgtcctaca tataaaatat caatatcatc ttcaacaaat
      901 tcattcccca tttttataat tctttcagta tttacagtgt taggtaacaa cacaaattta
      961 tcttttatta attgtttgta acgaaattct tccttaacgg aattcgaaac accaattact
     1021 gaattaaacc tagacgaaca taaagaatat ataatagttc ttatgtctag tttaggtaac
     1081 cacaagggat tattgtgcaa atgggaaagg atgatttgct tcttatatct acttgctctt
     1141 acactagcat taaaatcatg agcatgaatt atatctgggt ttaattcatg tacggctctg
     1201 tcaatttctt ttctcgaaaa tttttttaat ggtaagtaac ttaaacctct aatatttaaa
     1261 atatttgcta taggaccatc tgggctaacg taaatcatct caaatttttc gttattttga
     1321 aataaactca ttatttgaca aacaatattt tcagcgccag aaaatctatt actttgcaac
     1381 aaatgcataa tttttatttt tttcatatta atatcacttt caattcaatt atttacttac
     1441 aatttcatgg taaacattat ttatttggtt tatcacaact gataaatcaa aatcttttat
     1501 cttgtctaaa ttatgtgccc ccatagattc acacaataat ttgttatcag ccaatctatt
     1561 tatcgcacca gcaatttctt cttcgtcttt aggattgatt agaaatccac ccttaccatc
     1621 ttctataaga tctacatttc ctcttatatt accgacaata catggtaaac cactagccat
     1681 cgcttccatt aaagaggcgt tgaggccttc ccttaatgag ggtagaatat atatgtttgc
     1741 agcagctaat aatttgggta tatcacttct atatcctagt aaaaaaactc gctgatgtaa
     1801 tcctaattca ttagcaatat tttgtagtgt ctccctcatt tctcctaccc cagcgataat
     1861 ataatagata tctgtatgca gcggaatttt ttgtaatgca tgtattactg ataaatggtt
     1921 tttattctca tttagctctc ctacagataa aatgattttt gctgtatcag gtatattaag
     1981 ttctttacat atactttctc taaaattcct tattgatgtg gaagacataa acctatttgt
     2041 atctataccc acacctggaa tataataagc ttttccacct ttcctaagac gaagtttttg
     2101 agctaaagta taatcctcat gatttattgt aatcaaaaca tctgtataat gagctagcca
     2161 acgttccata ggataatata taaaccaatt tattttagga gcacctgaat aaaaatgaaa
     2221 tccatgtgcc tggtaaatta cttttttgac tttacattta agaccagcaa tccgtccgac
     2281 aacacctcca ataggtgtat tacaatggat aatatctatt ttttcatttt ttataatctc
     2341 tacaatctga tgataagctt ttatattttt taaatttagt gggtttctat aaaaatcaac
     2401 ttggtatatt tttattccat atttcacttc atcagctttt ctttcctcat cggatttgta
     2461 tttccagtta ccagctatat aaaattctaa acccgtttgt ttagctgcca tcatactagc
     2521 tttagaaaag ttatttactt tattagctac atttaaaata tacaataatt tcataattaa
     2581 ttcttctcca tataaaaccg atattcattc ataattcttc cttcattagt agaatattta
     2641 cgttctaaaa tgaactcatt ctttttataa aaattattca caacatcatt atttaatgca
     2701 tctgtttcta gtgcaatata ttcatatttt gaaaaatcaa ttctactctt taagttatct
     2761 ataagcatgg aacctactcc tatcgatttc tctttcggat taactccaat ggatgctaat
     2821 tccacataat tttcatcacg ttttgtttcg cttggtttta gaaaagctct aaaaagcctc
     2881 ataaggatat tgggtttctt tataaaggct acacaactat aaaatccaaa cggtattatt
     2941 cttttcttta tcatatactt atataaacca ctcatatctc cagaatatgc aagaaatcct
     3001 attggcttat cattttgaat agcaaccaat ataccagaat cattatattc tgtgtaagaa
     3061 cgatacatca attccaaaaa cttttttccc atgaaagtca aaaaaaaatt tgtaaatgtt
     3121 ctcatatgaa tatcgacaat cgaattaatt aactctttat ccgaactttt tatctcttca
     3181 atcaccaaaa ctcttatcct ccattcattt tatctgatta ataaactctc gttcaccttc
     3241 taagtcggcg tgatattctt taccaaatat aacaaaaata gttttacaca ttattttcaa
     3301 atcattcata caactaaatt cactgatact tttcaaattg taatacat
//
LOCUS       DACDRN010000134         3286 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDRN010000134 DACDRN010000000
VERSION     DACDRN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.136"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(561..1601)
                     /locus_tag="IWJ80_002756"
     CDS             complement(561..1601)
                     /locus_tag="IWJ80_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP9462412.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1950..2276)
                     /locus_tag="IWJ80_002757"
     CDS             complement(1950..2276)
                     /locus_tag="IWJ80_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462413.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2263..3051)
                     /locus_tag="IWJ80_002758"
     CDS             complement(2263..3051)
                     /locus_tag="IWJ80_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9462414.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT          992 a          551 c          459 g         1284 t
ORIGIN      
        1 tccgttgcgg gtaataccgt aacactgaag ggcacgcatt aaggcgtcat cttaatgctt
       61 tcctgtagga cactgacgga gattaccagt ctcccgagcc ttacaggggc ctttcttttt
      121 tttgtatttt caattatcac aatttatcgt ccaaaagtgc agaacgaaaa tcaattcatc
      181 acaagcatat cagacaatta aatttaaaac aactaaaaat atacaaaaaa gtgtaattga
      241 atattagcag cactgcctga aaagaagtaa tatcaggctt ttcagcttgg ggaaataagt
      301 gaattacctc tcttcatgaa ttattataac tcaaaaaagc acatatgcaa acactagttc
      361 cctatttttt tgaatgaaaa ataactaaaa tatctaacga ttatgacttt aaaacctacg
      421 tttgtgattg ttttcataaa cttttaatct aaaaactccg gaataatagc tatttatttg
      481 taacatttgt aataaaatta aaatcataaa aaagttctaa tttaaaattg atcattttta
      541 tagtgatcgc tttttatcaa ctactctagc caattttccg tactaactgt tggtaattcg
      601 ttaaatgttt ctctttgttc gtttgcaagc tttgccgcaa tagctttttc gacaaaccaa
      661 ttttttacat aaacgaaaat tagattctgc aaattgttta ctttttcagt tttttgtttt
      721 tgataagctt tcagcaaagt catatataat cctttgtcag catcaattcc ataagattcc
      781 agttcttcat aaacaacaac tgtttgctct aattcttcag cttttttctt tgcattatga
      841 atagtttgga cggttttttg agcgtcttcg taagtactag aaaatgtttt tatcaagttt
      901 aatacatttt ctggtacaaa tgtatttata ctgtcctctt tcatgatgtc gacgaagtta
      961 tctagtaaaa cttgattttg taactgatca gtttcagtgt ctattatgtg tctattagtg
     1021 tctatagaat tagaatagaa tagattgtct gcgattttcg caattacggt tgagtccttg
     1081 atatgactgg attttgccgt atttgcgatt ttcgcaatta cggtggtatc attgatttct
     1141 cgcggttttg ccgtatttgc gattttcgca attacggtgg tatcagtatt gtcttcctct
     1201 tccatgattt taaagacgtc ttgattcgtt acttctggct tgcctaagta caataaatta
     1261 ggcatggatt caatcttacc gtcacgttta tttactcttc ctctcttctg tttcagaaga
     1321 cctgcttttt ctaattcctt tttgatttta gatactttac cttcgcggca ttgtaacaat
     1381 ttcattaatt cttcaactgt gaatatgaaa aatatattat catcagaatc tacccagtta
     1441 tttttgactg aataatcaaa tcgatcttta agtagcatat aagcaatttt tgcatcattt
     1501 gatagatcca tgtatttttc gttagtaaaa aataccttag gcatttgata atatttttca
     1561 cgataaattt cttttgattt gtaataatta aaatcattca tagccaatcc tctttctgta
     1621 ggatagcgtt ttgttcaaaa ctatgttaca atgaattaaa caaatatatc tatccactat
     1681 atatttttct actgctgata agtcgccaaa ctttgttaaa gcagtagtct ttttttatct
     1741 ttttttagtt taccttaaaa aataaattaa agcaaaccgc tctatttcaa ggtttaagct
     1801 catagtaact atatcacgat attacaataa tattatatca cgatatcgta atattacaat
     1861 atgaagataa gcgacttatc agctttgata ctactgattt ttataaaaaa aatatcacga
     1921 tattatgata tcgtgatatt gaatgtaaat taaattttat tttgtttctt tgctagttta
     1981 tatccttcaa tcaagtattc ttcaggtatg gactttaata gcgcatcttc tataacgtct
     2041 ggtttacttt tgtctgattc agcaatagtc gtccaatatt tgagtgctga cattagaatt
     2101 ggatttatac gaatgctatc tgctgttctt acactacttc cttgatcttt tatgacttta
     2161 cttatatttt tcttggttaa ttcgatttct tctacttctt ttgatttaaa attcttttta
     2221 tttttatttc ctagtaatct acttactgca gcttgactgc gattatcttc tgccatcaaa
     2281 atcaccaact tcttttaaat attcttctgt aaatgcttta tagtcatctt cagctgttga
     2341 gggtttatta aaattgatta gatctgatcc agcagcttgc gcttcgtcaa cggttattgt
     2401 cctacgaata taagttttga aaatttttgc accaatgact ctagaaattt cggttgctgc
     2461 agacgtcatt tctttagaaa tgttataacg cttattatac ttagtaaata gaactcctcg
     2521 tacttcaagc ttgtcgttcg tatattcttt taccgtttca atagtttcac ttaactttat
     2581 tagtcctttt aatgagaaat acgcagcttc aactggaatg ataatttcat cagaaacagt
     2641 aaatgcgttg actgtaagca ttcctaaact tggtggacaa tcaattataa tataatcata
     2701 gttgtccttg attggttgaa gtactctttt aagcctgtgt tcacttccta cttgagtaaa
     2761 ttccctctct gcagaagaaa gggctatgtc ggctggcaaa acatcaattc ctttatagtc
     2821 cagaatagca tctgtaatgt cagcctcccc ttttaatact tcataaattg taggttggtt
     2881 tgttccatct atatcgaaac ctacattatc tgtagcattt ccctgaggat caatgtccac
     2941 taaaagtaca tttttaccgt aaacaaattt caaacatgct gctaaattaa cgctagtcgt
     3001 agttttagaa actccacctt tttgattggc tactgacaat attcttgcca ttaaaatatc
     3061 ccccattcat cttatattat aatataatta tatcataata ttgtgatatt gcaatatcgt
     3121 atatgcatta tattgtaata ttgtgtgatt attttgtttt aaagatatat atcggttcgg
     3181 atataaagat tattcatatg ttattcgaaa ttaaaataat cactttttat tactttaaaa
     3241 ttttttatta atgataagaa atatgttttg cgagcagcaa ctagaa
//
LOCUS       DACDRN010000135         3275 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDRN010000135 DACDRN010000000
VERSION     DACDRN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.137"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..466)
                     /locus_tag="IWJ80_002759"
     CDS             complement(<1..466)
                     /locus_tag="IWJ80_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP9462415.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTG"
     gene            1160..1546
                     /locus_tag="IWJ80_002760"
     CDS             1160..1546
                     /locus_tag="IWJ80_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9462416.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1644..1835
                     /locus_tag="IWJ80_002761"
     CDS             1644..1835
                     /locus_tag="IWJ80_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9462417.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            2349..2681
                     /locus_tag="IWJ80_002762"
     CDS             2349..2681
                     /locus_tag="IWJ80_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9462418.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2591..2794
                     /locus_tag="IWJ80_002763"
     CDS             2591..2794
                     /locus_tag="IWJ80_002763"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9462419.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            2978..3133
                     /locus_tag="IWJ80_002764"
     CDS             2978..3133
                     /locus_tag="IWJ80_002764"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP9462420.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT         1210 a          529 c          491 g         1045 t
ORIGIN      
        1 ttcctgtata ttcaattaac ggttcatgga atagatcttc tatttttcca gtaaaaaaat
       61 aatgaactaa aatcagaata tctcgaatgt ctcctaaaga atacaaagtc aaattttgca
      121 atgaattata gagtcgttct ctatcagcca ttgcaacatt caaattgttc atttttagtt
      181 taaaaaaatc tttatcaata ttatttgctc taaatggacc aaataagtaa tatctggttt
      241 taaacatgta tagaagaaac atctcattaa aatcaccagt aaagaaatgg aaatctttag
      301 tttttttaat attatcagtt agtaactttt tataatcata aaaaaaataa gtagttctat
      361 ctgagcgata ctcttttata atactgaatt cggaattaaa aaccacgata ggtaacttta
      421 aacaaacatg aagagacttt aataagactt ctttatctga aaacactttt gtttctctct
      481 ttcctttcta catcattaaa tagtatttta tacgttatct aaaatactat tcctctgtca
      541 aaataagcta acttacttca ttataaaata tccacttatc gctttcatgt atattccacc
      601 gtaaaaagta cattttatca ccgagaactc atcatttttc atcatgatga atactattta
      661 catatatcta gttcaaagac aataattatt atgatccgtt attatctaag cactatacag
      721 ataataggaa tttttcgttt ctaaatactc caatttggga atccaataaa aattttcgtt
      781 caaatacgtg tctattcgaa ctcttttcta gttgttaatt atgaaggggg aagaaataaa
      841 aaatacaatc tatacacgaa taagaatatt atccaaaagg aggttttaga aaagatttat
      901 tatacgtaaa aagctcaaga cattttctta gccataagga catgtttgat atttaagaag
      961 atatatgaat tttcttttct aagtataaag ataactatcc attatcaact aaaaaaacat
     1021 ttttgaaaat aaacatcagc agtcctatca atgcatgtga gcttatagaa gttattcgca
     1081 gcgcagcata attgcattag cttcttttcg tgttttttgc ctcttttccc tttaaatctt
     1141 aatggaggag gtgtgcaata tgtcagataa cgacgaacgc gtaaatgccc agttgattcg
     1201 attctttgaa aaggtcattt tgaacaccgc taatacttat tataaaaaac aagaagaaat
     1261 tcaaaaacat gaacaacttg atcaactccc accatatttg tttactaaag atactataaa
     1321 atttaaacaa ccgattaatg ttttaaatgt gactctcctt gttgaagata taaaattagc
     1381 aaaaatcctt cctttgttga aagaacctga acaaattttt cttatcgaaa aatttatctt
     1441 taataaaact gataaagaaa ttggaaaggt tctggggatt acaagacagg gtgcgacaaa
     1501 tttaaaacat cgactttaca aaaaaattca aaaaatgctg gtttaatatc tgcataataa
     1561 ctaattttgt atgcttcttt gctcgtcctc ctccttccca aaaaaataaa tagattataa
     1621 gttcatggga agaaggtaaa tttatgaata ctttaaaaga agatttcatt ttagcaaaga
     1681 atggaaatga agaagcagtt gtggccattc tcaaaagatt tagcgctttg gtccataagc
     1741 aatcctggag aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat
     1801 atctagctat ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata
     1861 agtaaaaaga tagtttcaaa tttcgaacaa taaatttgaa gccatcttct ttataattat
     1921 aaagtatcga ttttacagcg ttctataatg attcatagaa tctaaaacga atgattttaa
     1981 ttattaacgt tttttaattc gtttttagct taaaaataac ttttgtcaca gtctcactcc
     2041 taaaattact ttatttacgt acgaagtggg caggaggtcg aatccagaac tgggccatgg
     2101 tacttcaacg aggacttctc ctctagaatt gaaaagcatg cgatttacct tccgtaacga
     2161 cggtagattt attcaactat gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag
     2221 agaattttgg ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt gacaaaccct
     2281 gatactaagt tgggctatga tacacttttc ttgactatca gttacgttac cggaaggaat
     2341 gaactctatt gaaaaaaaca tatgatcgta aatttaaact aaagatttca caggatatcc
     2401 ttgagaaaaa agtaactacc aagaaaattg ctgaagaata caatatctct cgtccaacaa
     2461 tttcccgatg ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa
     2521 aaagattacc tgataaagca gatttctaca ttcttgaaca ggaaaataaa cgactaaaag
     2581 aggaagacga gtgttctacg aacacaaaga tcgatacgga tctccaggga tcactcaaaa
     2641 actttacgat gaaggaatcg aaatcaataa acgtgttgta gctgttctta tgcgtgaaat
     2701 gaatttatgc acaagaggat ttcatcgacg caaatcttct catggtagag aaaaaacaat
     2761 caaagaaatg gtaaaaaaaa tgttgaatcg ataatttgaa cagaaccaaa ttgatgctgt
     2821 ttgggtgacg gatattactt acattccatg taatgacggt cgcctctatt tgtccaccta
     2881 tattgactta gcaactcgta ttctacgtta ctatatggtg gattcacaca tgaaaaaaga
     2941 gattgtcgtc cgtccattac aaatttatta aggagaattg cctacggtaa ttcattccga
     3001 tagaggtagc cagcatcggt cttttattta tcaagagctg ttgaccgacc accacatcac
     3061 acattcaatg agccaaactg ggactcctgt tgacaatgca gtaattgaag cgtttcatcg
     3121 atcaatcaaa tgagaattaa tcgactccaa caaatataaa agtaaaatag agatgaaggt
     3181 gttaatccaa gattatttag aagactacta cccaaacaaa agaatccata caaaatttat
     3241 gatgactcct tgacaatttg aagcgaagaa aataa
//
LOCUS       DACDRN010000136         3182 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDRN010000136 DACDRN010000000
VERSION     DACDRN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.139"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(61..2424)
                     /locus_tag="IWJ80_002765"
     CDS             complement(61..2424)
                     /locus_tag="IWJ80_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAP9462421.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2872..3093
                     /locus_tag="IWJ80_002766"
     CDS             2872..3093
                     /locus_tag="IWJ80_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9462422.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
BASE COUNT          863 a          664 c          575 g         1080 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca
     2641 tcatgcataa tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct
     2701 ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt
     2761 caatatgtga attggtatcc cagctactat gctaatttct acagggatta tccaaaattt
     2821 ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt
     2881 cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt
     2941 ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc
     3001 ggtatcttga tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa
     3061 ttttttactt ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg
     3121 gaactcttag gtaatacgtc ttaatgaaaa caaaaaaatc ccgcaacccc tgatttcaca
     3181 gg
//
LOCUS       DACDRN010000137         3109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDRN010000137 DACDRN010000000
VERSION     DACDRN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.141"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..773
                     /locus_tag="IWJ80_002767"
     CDS             <1..773
                     /locus_tag="IWJ80_002767"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP9462423.1"
                     /translation="FQVYLQRVYILSTGVIIGHYAEMGLNRIAYAGLMGDFSFAGLYL
                     ATGCLYAFLKYVDLKNWKLLFFIIIEVFLLGAILCVSARTGLVSLFVTMTLYYLFNIN
                     RISVPHLLVLIGGIITTPYILVMLMAGRSGQSLLDSSGRLENYMSSLNAFSDKYLIGY
                     GLGLNNLYTLLGVAVPHNFFIQYLLQTGIIGTLIILSGFFKFTYEELKKSGCYKWLFL
                     MIVVGAMFIPDIVSSRFLYAIVVLCMISASERTIKKES"
     gene            784..1245
                     /locus_tag="IWJ80_002768"
     CDS             784..1245
                     /locus_tag="IWJ80_002768"
                     /inference="COORDINATES: protein motif:HMM:NF012360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAP9462424.1"
                     /translation="MRRKAEKWADKACRQYRNGQNKMSKISCYILRIIYGCEFYGQVD
                     FSSNAQLVHNGLGCVFHPKTKIGNNCKIYQNVTLGGNGKIINGKETVGAPTLEENVAV
                     FAGACVLGPINIGHDSIIGANAVVTKSVPPYSLVYGNPAIITELKFDYKFD"
     gene            1304..2761
                     /locus_tag="IWJ80_002769"
     CDS             1304..2761
                     /locus_tag="IWJ80_002769"
                     /inference="COORDINATES: protein motif:HMM:NF024832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAP9462425.1"
                     /translation="MGNNIHGKTAKALKWSSLAEIIAKIITPISNMILARLLTPDAFG
                     VVATVTMVISFADMFSDSGFQKFLVQHEFDNEEHMFSSTNVAFTSNLGISLLLWLFIC
                     VFNNKIAHLVGNSGLGLVIIVAGVSLPITSFSSIQSAIFRRNFDYKILCYARIISALI
                     PLVITVPLAIIGLSYWSLVIGSIIGNLVTAAILTKLSEWKPKLFFSKKEFYEMFSFSA
                     WSLVESLGTWLSSYIGTFIVGGILSVYYLGIYKTTMTTVNGIFAIVTSATTTVLFSTL
                     SRLQNSKTEYDKTYLDFIKIVSIFIIPLGIGIYVYRELVTNILLGSQWKEAVPFVGIY
                     GLMSCFTLVLGQYASEYYRGLGKPKANVLMTILHLAVLIPVLIIYSRKGFIELAYGRS
                     FVKIEQIIVFWIILWFGFKFNPFKILKQVWKSCASALLMGVLAKIMLEFCTNYVMQLL
                     SVCLCVILYFFIYSCIFKGKTEIINMVRMFTGKGY"
     gene            2796..>3109
                     /locus_tag="IWJ80_002770"
     CDS             2796..>3109
                     /locus_tag="IWJ80_002770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9462426.1"
                     /translation="MKKVTILIPGMYYGGMERVAFITRQILLKNNYNVSFVTLFDNDA
                     DYQPNFEYLSLKCDIKPSKFGKVINIFKRVYKMKSVKKKLKTDITISFGQSCNLVNVL
                     SK"
BASE COUNT         1057 a          350 c          572 g         1130 t
ORIGIN      
        1 cgtttcaggt atatttgcaa cgagtatata ttttatctac tggtgtaata ataggccatt
       61 atgcagaaat gggtttaaat agaattgcat atgcaggttt gatgggagat tttagttttg
      121 cagggttata tcttgctaca ggctgtctat atgcttttct aaaatatgtg gatttaaaaa
      181 attggaaatt attattcttt ataataatag aagtattttt attaggtgca attttatgtg
      241 ttagtgcaag aacagggtta gtttccttgt ttgtcacaat gacattgtat tatctattta
      301 atataaatag aattagtgtt cctcatcttt tagttctaat aggtggaatc ataactacac
      361 cttatatact agtaatgtta atggcaggtc gttctggaca aagtcttttg gattcttcag
      421 gcagattaga aaactatatg tcctctttaa atgcttttag tgataaatac ttaataggat
      481 atggattagg attaaataat ttatacacgc tactaggtgt agcagtacca cataattttt
      541 ttattcaata tttattacag acaggaatta ttggtacgct aattatttta agtggcttct
      601 ttaaatttac gtatgaagaa ttaaagaaat caggttgcta taaatggtta tttttgatga
      661 tagtagtggg agcaatgttt ataccggata ttgtatcttc gcgttttctt tacgcaatag
      721 ttgttttgtg tatgatttct gcatcagaaa gaactataaa aaaagaaagt tgaggtagaa
      781 aaaatgagaa gaaaagcaga gaaatgggca gataaagctt gtagacaata caggaatggt
      841 caaaataaaa tgtcaaaaat atcctgctat attttaagga ttatttatgg atgtgaattc
      901 tacggacagg tagattttag ctctaatgct caactagtcc ataatggtct tggttgtgta
      961 ttccacccta aaacaaaaat cggtaataat tgtaaaatat atcaaaatgt gactttgggt
     1021 ggaaatggca aaataataaa tggaaaagaa actgttgggg caccaacgct agaggaaaat
     1081 gtggctgttt tcgctggtgc atgtgtttta ggaccaatta atatcggaca tgattcaatc
     1141 ataggtgcaa atgctgtagt tacaaaaagt gttccaccat atagcttagt atatggaaat
     1201 ccagcaatca taacagagtt gaaatttgat tataaatttg attaaaaagt aatgtttaat
     1261 atgcgaaagg ttgctatatt tatttagcaa aatggggtaa taaatgggaa ataatattca
     1321 tggaaagaca gcaaaagctt taaaatggtc atccttagct gaaataattg caaagattat
     1381 aactccaatt tcgaatatga ttttagctag attattgact ccagatgctt ttggagtagt
     1441 agccactgta actatggtta ttagtttcgc tgatatgttt agtgattcag gatttcaaaa
     1501 atttttagta cagcatgaat ttgataatga agaacacatg ttttcatcaa caaatgtagc
     1561 ttttactagt aacttaggta tttctttatt attatggtta tttatttgtg tgtttaataa
     1621 taaaatagcg cacttagttg gaaattctgg acttggatta gtaattatag tagctggagt
     1681 ttctcttcca attacttctt tttccagtat acaatcggca atttttagac gaaattttga
     1741 ttacaaaatt ttgtgttatg ctagaataat atctgcattg atacctttag ttattacagt
     1801 accgttggcg ataattggac taagttattg gtcactagta attggatcta taataggaaa
     1861 tttagttaca gctgcaatat taactaagtt atcagagtgg aaaccaaaac tttttttttc
     1921 aaaaaaagaa ttttacgaaa tgttttcctt tagtgcgtgg tcactagtgg aatcattagg
     1981 tacatggttg tcatcttata taggtacttt tatcgtagga ggcattttaa gcgtatacta
     2041 tttgggaata tacaaaacta caatgacaac ggttaatggt atttttgcaa ttgtaacttc
     2101 agcaacaaca actgtattat tttcaacatt atctcgttta caaaatagta aaacggagta
     2161 tgataagaca tatttagatt ttattaaaat tgtttccatt tttattattc ctctgggtat
     2221 aggtatatat gtatataggg aactagtaac aaatattcta ctcgggagtc aatggaaaga
     2281 ggctgtacca tttgttggga tatatggatt aatgagctgt tttactcttg tcttgggaca
     2341 atatgcctct gagtactatc gtggtttagg aaaaccaaaa gcaaatgttc ttatgactat
     2401 cctacattta gcagtattaa ttccagttct aataatctat tcaaggaaag ggtttataga
     2461 actagcatat ggtagatctt tcgttaagat agagcagatt atagtctttt ggattatttt
     2521 atggtttggt tttaagttta atccatttaa aattttaaag caggtttgga aatcttgtgc
     2581 tagtgcattg ctaatgggag ttctagcaaa aattatgttg gaattctgta ctaattatgt
     2641 tatgcaatta ctttcagtgt gtctttgtgt aattctatat ttttttatat atagttgtat
     2701 atttaaagga aaaacagaaa taattaatat ggttagaatg tttactggta aaggatatta
     2761 agagagattt ttatggcaag gttgtgatta ggaaaatgaa aaaagtaaca attttaattc
     2821 ctggaatgta ttatggtgga atggaaagag tagcttttat aacgcgtcaa atacttttga
     2881 aaaataatta taatgtatca tttgtaactc tttttgataa cgatgcagat tatcagccaa
     2941 attttgaata tttatcatta aaatgtgata taaagccatc aaaatttggt aaagtaatta
     3001 acatttttaa aagagtttat aaaatgaaaa gtgtcaaaaa aaaattgaaa acggatataa
     3061 ctatatcttt tggacaaagt tgtaatttag taaatgtttt atcaaagag
//
LOCUS       DACDRN010000138         2944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDRN010000138 DACDRN010000000
VERSION     DACDRN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.142"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            14..1141
                     /locus_tag="IWJ80_002771"
     CDS             14..1141
                     /locus_tag="IWJ80_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9462427.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..2501)
                     /locus_tag="IWJ80_002772"
     CDS             complement(1359..2501)
                     /locus_tag="IWJ80_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9462428.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2506..2691)
                     /locus_tag="IWJ80_002773"
     CDS             complement(2506..2691)
                     /locus_tag="IWJ80_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9462429.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT          975 a          537 c          462 g          970 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt
     1981 tttattttac ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt
     2041 ttaaaaacta ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc
     2101 tcaatgccct tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga
     2161 attggtcgaa tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata
     2221 tgatctgttt tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac
     2281 tctcgataaa aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa
     2341 ctttgtttta actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg
     2401 gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg
     2461 cctctttttc tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga
     2521 aatatggaga acttcttcta aaattgaaat aggaattctt tttatttttg aattacgata
     2581 aaaaaagtat cctttctcaa ctaaaagttc tttacattcc ttgaagattt tattagcata
     2641 gtactgagaa aaacctagat taatcaaatc ttttcgagta gctgttttca ttatagcacc
     2701 ttccttactt tttttcattt atttctatca gctgcttttg tcaaaagcac ataggaattt
     2761 cacctctccc caagtttacg gcgagcctta cggaagtatc attatcccaa ctttaaatca
     2821 tcgcttttta ttgactataa aaattatttt agaatattta tcgctccttc tttacagcaa
     2881 gaaatcatgg cgcaactaaa atatgctcct taaagtgtta acgtactgat agaatctatt
     2941 taat
//
LOCUS       DACDRN010000139         2867 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDRN010000139 DACDRN010000000
VERSION     DACDRN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.143"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(130..492)
                     /locus_tag="IWJ80_002774"
     CDS             complement(130..492)
                     /locus_tag="IWJ80_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462430.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(566..946)
                     /locus_tag="IWJ80_002775"
     CDS             complement(566..946)
                     /locus_tag="IWJ80_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP9462431.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1028..2203
                     /locus_tag="IWJ80_002776"
     CDS             1028..2203
                     /locus_tag="IWJ80_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9462432.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2280..2864)
                     /locus_tag="IWJ80_002777"
     CDS             complement(2280..2864)
                     /locus_tag="IWJ80_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9462433.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          893 a          555 c          482 g          937 t
ORIGIN      
        1 acatattttg aaaataaaat gcactaaaaa actatcttta gcagaacatt tcattgtatt
       61 ttttggacca aacaataaaa aaagaagttt gaaaaaaatt caaacttctt tgttcgctta
      121 atctgtattt tagaagaaag gcactccctc tacttcgtaa gttccaagaa cagagcgcat
      181 ttgacctggt tcttcccaaa tatatccttc gcctaattct tctctttgat tcttttgttc
      241 agtttcaaag acaaacaacg gaacattttc ctctttaata tgtgtatttg tcaattccat
      301 caaactaatc ttgctaacat cgatatgaac tatttctttt aagaaattaa gtatattcgc
      361 taaaccagtc tttccttcag aaagttccgc taacgcaaat tccaacttgt catcaattga
      421 atggaccaaa aattttttag taccatcctc caaatgcaac ataattgtac cagcaactct
      481 tttgcttgcc aaaatttcca cctcgatcaa cttgttctat ggcttacata aaaattctag
      541 cacattttca gaaaaaatac actttttatt tcttattttc tttccgttct agttcagata
      601 atgcttcttc tatccaaata ggtaactgct ccgcaatagt ggtaaatccg atcttacgat
      661 ttttattcgt gaaatacctt tttctgcgat atggaacagc tggatttgtt ttttctaacg
      721 ttcttaaaga taaactgatt tttttggaat attcatcaat gtcgatgatc tgaactgtta
      781 ctggctgtcc gacttttaac aatgaatgga tatttttcgt ataaccggat tgtacttctg
      841 aaacatgaat aagtccttgc gtctcgttat caagagaaac gaatgcgcca taaggctgta
      901 tccctgtcac ttttccttca attatttgtc caattttata agtcatctac ttccctctct
      961 tcgcatttat cattatactg taaatagctc aaaaaaagaa gcgacgaaaa tgactggagg
     1021 tcttaagatg atcgaatttg ataaacagat ttcaagaaaa gatacagaaa gtgtaaaatg
     1081 ggacgcaatt gctgagacat atcaaatgga tgacttactt cctttgtggg tagcagatat
     1141 ggacttcttg tctcccggtg gggtagctaa agcgtttagt gaatacatca aacatggaat
     1201 ctttggttat gccactctgt ctgataaact atatcaggct atcattcatt gggaacgcca
     1261 acaccatgct gtggagttga cgaaagaaaa tatcgttttt acaagtggtg tgttaaccag
     1321 tttagctgca gcagtacaaa cttttacaaa accaggcgat tctattttga ttcatgaccc
     1381 agtttatcct ccattttcct ctattataga aacaaatcaa cgtcacatcg ttcgaagctc
     1441 tttgctagaa aaaaataatc atttcatcat ggacttgtct gacatggaga aaaaaatcga
     1501 agaaaacaat gtaaaagcaa tgattttatg caatcctcac aatcctggag ggcgtgtttg
     1561 gagcaaagaa gaattgaaac agttaagcga attgtgttta aaacatcaag tgattctgtt
     1621 tagtgacgag atccatcaag atttaacttt attcgatcat acatttactt ccatgctaac
     1681 gattgatcct aatttagatg atctactagt tgcttttact tccgcaacca agactttcaa
     1741 tctagctgca atcaaaaatt caatggtatt tatcaaaaat ccagagatga aagctgattt
     1801 tgaaaaacat ttagtgatga accaacagca cgaaatcaac acttttggac tgattggaac
     1861 ccaagcggct tatgagacgg gtggagaatg gttaagccaa ctgattcctt atctagaaaa
     1921 aaatgttcaa acagttaatc agttttttga acagtatctt ccgaaagtga gaataatgaa
     1981 ccctgaagga acttacttaa tgtggttaga ttttaccgcc tatatttcag acgaccaaga
     2041 attggagaac actcttgttc ggaaaggaaa agtcgtatta aatcctggca tcacgtttgg
     2101 tccttccggt catgggcata tgcgcttgaa tattgcttgt ccagaagaaa ctttactgga
     2161 aggacttaat cgaatcaagt atgctttaga gacaatcgat taaatcattt attcaaaata
     2221 aaaggacgag accttactag gtttcgtcct ttttcataat tactccgtac cataccaaac
     2281 tattctgaaa tttcaattgt ttcaatcact acatcatgta caggtcggtc ttgtggacca
     2341 cgttgtacat ttgcgatttc atctactgtg tccatccctt ctagtacatg tccaaagact
     2401 gtatgtcgga aatctagcca aggagttccg ccacctttat acgcttcaat gatttctgat
     2461 gggaatccag caccttctag ttgtccaagc atattagctg gcacatttgt gttatttaca
     2521 ataaagaatt ggcttccgtt tgtgtttgga cctgaatttg ccatagataa agctccgcga
     2581 aggttgaaca gttcacgact gaattcatct tcaaaacttt cgccataaat actttcgcca
     2641 cccattcctg ttccagttgg atcgccgcct tggatcatga aatcaggaat aacgcggtga
     2701 aaaatcacgc catcataata accttttttg gaaagttcaa tgaaattttg aaccgtcttc
     2761 ggtgctagtt cagggaataa ttgaacagta atgtcaccac ggtttgtttt gataacagct
     2821 tttggtcctt tttcactttt taaatctagt tgtgggaatg ccatatt
//
LOCUS       DACDRN010000140         2850 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDRN010000140 DACDRN010000000
VERSION     DACDRN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.144"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..2269)
                     /gene="esp"
                     /locus_tag="IWJ80_002778"
     CDS             complement(<1..2269)
                     /gene="esp"
                     /locus_tag="IWJ80_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP9462434.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
BASE COUNT          830 a          555 c          428 g         1037 t
ORIGIN      
        1 aaatatcttt acttacagtt actgctaaat cggtcgtgcc tacagaacca tcttggtaaa
       61 caattttaat aggaattgtt ccagtaatcg gctccgattc tcctacacct ttagctggag
      121 tgaatgtaac aataccagtg tttgcatcaa cacttgcatt accgaagtac ttattataga
      181 catctgaaat tgaaaaggca tctttagcta aaggaacatc tggtttatca aatgtaggtt
      241 ttccactttg aactgtcgtt tctggagttg ttgttatagg tacgtatgtt gcatcatttt
      301 ccatagcttg agaagctttt acagcaaact ctacatccac gtcgaaagtt cgatttccga
      361 tcttaacagt tacaatacct ttcgtactat cagtaacgtt agaagtatca ggctctgttt
      421 tccatgaata ctctgcatct tcaggtaagt ttttattatt ccgaattgat tctttagcat
      481 ctggtttaaa tccaactacc acggtttgtt tatcaaccgc ttttggtgat tccttaataa
      541 cggttgaacc ttcttctggt ttatcaaaac ctggagaaac gatttggatt tttaattcat
      601 ctttcgcgat taatttactt gaatctacac ccgtaaattc aaattctaca gtatctgaaa
      661 ttggagcccc atctttttca tttggagcga tagttttttc tgctaataca gtaccttctt
      721 tatcaacaag ttgggctttg tgacctgttc ccgctaactc gtggatgaat actttcccct
      781 taactgttgt gtcaacatca tatacttctt ctaattcagg tttcccgata tatggattcg
      841 tgtctccgct ctcttcttta tcgtcagggt ttccatcacc atcaatatct tgcaaataat
      901 aataacctgt tccataagtg ttctggatta attttttatt cgtatccgac aaccaagaat
      961 caaagatgaa atcttcacca aatggtttat tttcttcaac ttgtgctgtt gctttcatat
     1021 cttctaagat ttcatctaat ggcttagtca attgtaaaac atatcgaata acgactggac
     1081 gtggacttgc attagcaaaa tcgataagtt tagttaaatc attattagtt attgaaccta
     1141 tgccataatt ttcatgacct tcccgtcgct cgattgctaa atcagctact tttgttccgg
     1201 attcaaacga aacatcatat gttactttgt cagcctgaac catgtgcatc tgtatacccg
     1261 ctacatatgg aactaatcgt tcatcaattt tgtaattagc ataccaaggc gtgccctttg
     1321 cggaagatga atatgagaaa ttataatttt tcgttaactt atgatctaag attatagctc
     1381 cattagcacc tgtggatcct gaaccatctt ttgtttgagt attaggtttc tcatattttg
     1441 cacttactga tccatgttta aactggtcag gattattttc agatacatct aagctattat
     1501 agaaatcatc tgaatctgtt aaaaagtagc ccgtactatc aatacctggt acaattttat
     1561 catcgtcttg atgacttacc aagtagattc gatacattaa acgatcggtc ttgagattgt
     1621 ctatgcctat ctctttaaaa atttcatcta ggctcttttc aaacgtaatt accccattag
     1681 gagcggtttg cgtatcggtt gtttctgccc cagcaaataa tccatcactt gcacgaatat
     1741 aattgacctt ccaaatattt gtaggtctac ccagcgaatc attaattctt acaaaggtcc
     1801 gtctagaaga tccaattcct ttctttggat cgacctgaat tttttctaca tatttagcta
     1861 atcgttcatc taattgaaga cggatttccc agtttttagt tgatgctgca atattatgtt
     1921 ttccgtaaac ttcaaatcca attgtattgc cttttagtgt gtctccagtt agttcttgag
     1981 gactaaattg taaatccgaa aagttataat ttttcgtata accaggattc tcctgaaaag
     2041 tattccccga agctactttg tcacctactt gtacggagct tatcgctgtt tcaagtgggc
     2101 tgcttggaga aatttcatct tgtgctttaa ctagttcagt tgttcccaag acaagaccaa
     2161 caccaatcaa aacagatgca accccaacac ttaatttttt gatactgtac ttcaatactc
     2221 gtttctttgt tttttctaga aatactctct tattattctt gctaaccatt attcaaatca
     2281 actcccttaa taattacttt cctagtaaga ataaaagata aatctgatac gtattattca
     2341 cagaatagga atcttactaa ccttatgtta actaatttac ttagagaatt tcatgaacat
     2401 tacaacaaaa aaaagtacat ttcttcatgt ttttttcgga tatttgtgct ttttaaccat
     2461 tcagcagctg aaaacatata aaaagatatg tatgtattct ttctgaaatt aagttcattt
     2521 ctatgcgatg aagcatgttt tcaaaatatt actctttctt agattaaact tcagagtatt
     2581 caaatagcgg aataaggagc cttatcccgc attactgaat gagactgtga cataagtaaa
     2641 aaggtgactt caaattcctt cttcggaatt tgaaatcacc ttctttataa ttataaagta
     2701 tcggtttcac agcattcctg aatgattcct agaatcttaa acgaatgatt ggagttatca
     2761 acattttttc actcgttttt agcgaaacaa aaaatgacat ttgtcacagc ctcattaaat
     2821 tattgattct ttttctttat aattgaattg
//
LOCUS       DACDRN010000141         2602 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDRN010000141 DACDRN010000000
VERSION     DACDRN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.146"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..2206
                     /locus_tag="IWJ80_002779"
     CDS             <1..2206
                     /locus_tag="IWJ80_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP9462435.1"
                     /translation="TNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGI
                     NPGGFDGVYTPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSK
                     IRKMQQWLNVNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTS
                     KTYPVSEGNSGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTE
                     IADVVVIKGLLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGK
                     DERNKYLTTEELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIP
                     LTTVIYFAVDLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYA
                     SFAFVSDMSTGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNEL
                     SNDTNNDNYIAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIIS
                     SPVTEHDRKIYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTIND
                     SVSDFAGWLGDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYT
                     LAKRNSDSTALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDIL
                     GVGIISFFIDKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            2286..>2602
                     /locus_tag="IWJ80_002780"
     CDS             2286..>2602
                     /locus_tag="IWJ80_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462436.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEY"
BASE COUNT          890 a          329 c          526 g          857 t
ORIGIN      
        1 tactaatcta gtagctaatt ttggacctac cactgaaaaa atgtacgata atcaggttac
       61 tcctaaatgg ggcaaaaatc tacctaaaaa tattgtattt cttattcaag gtgccttttg
      121 gtgtaagggg attaatccag gtggcttcga tggggtatat actccatatt tagatactgc
      181 tgtaaaagag ttgcagactg atgcagggtt taatggaagc gctgtaactg gagttttgga
      241 tgcgaaatgg gccaaagcgt tatttgatat gagtgctttt gttcttgttc ccggcggaga
      301 tagtaaaatt agaaaaatgc agcaatggtt aaatgtaaat tatttagaat acacgggaat
      361 aatgccttgt gatggtattt atcaaagaaa ttcaaatcaa gcattaattt ttgcattaca
      421 agctgaacta ggatattctc ccagtgaagc aacagggtct tatggtaatg ggacaactag
      481 taaaacttat cctgtgtctg aaggtaactc gggaaactat gtaagaataa ttcaatatgg
      541 attatatgtt aatggttatt ttgaagacgg tacattcgat ggtattttca ctaaatatat
      601 gggtttagaa gtactggcat tccgaaaatt tatggtattg cctgaatata ctgaaatagc
      661 agatgttgtt gtaataaaag gactattgtc tagcgctgga gatattagaa gatctgcaga
      721 tggcgcagat acttctacgc aattaactag atctcaaatc caaacattgg ttgataatga
      781 tataaaaata gttggtagat atcttactgg tactgttgga attggaaaag atgaacgaaa
      841 taagtatcta actacagagg aattgaataa tattttttct aaaaatttat ctgtgtttcc
      901 tatctatcaa gatggaggtg cagcattagc ttattttact tatgatcgtg gcttatcaga
      961 tggtaagaag gctatagatg cagcaaaaaa tctaaatata ccattaacaa cagttattta
     1021 ttttgctgtt gatctagaca tgttagggga agaaattctt gcatatgctg gagaatattt
     1081 taagggggta tctgctgtaa tgtcatatag cgggtatcaa acgggtgttt acggaacaag
     1141 aaatgtttgt tctcaaataa tcaataacgg gtatgcttcg tttgcatttg tttcagatat
     1201 gtcgactggt tatagtggga atttaggttt tcctatgcct agacaatggt cttttgatca
     1261 atttgttgaa tttaccatag gaagtggcaa cggagctgtt ggtatcgatt taatcgctac
     1321 atcgggtaga gatagcggat ttaatgagtt atcaaatgat actaataatg ataattacat
     1381 tgccaaatac aatcagaagg ttataaatca gatggtacgt gcgtatcaat atctatcaca
     1441 agctggtctt gataatcctt ggaaccctca tttgacattt tatagatatg taaattattc
     1501 tggtttgtca tgggacatca tttcttctcc tgtaacagag catgatagaa aaatttatga
     1561 tgaatatagg aataaattaa ctaatagcga gggcctgtat aattatttta tagatcctaa
     1621 tactggttca ataataggtt tgcctcactt aattgtgacg ttacaatcac agatgtttct
     1681 agccgacaca atcaatgata gtgtttctga ctttgcaggt tggttaggag atttaatgac
     1741 ttgctggggt gaagttaaga aattaggaat acaaatggag caaggtgtat ttgcattagt
     1801 aggtacggct gcaggtggaa catttagtct agaagattta taccaagatg ttgacgcggt
     1861 aaatgtttat accttagcaa aaagaaattc tgattcaaca gctttatcgg ctttaaaaac
     1921 gtactattgt gataagggtg ctattgatag atttaatcaa tttgttaaga acagatttgg
     1981 ttcttcagat aaaatcgctt cagtaacaaa tagtttatta gaaaatgacg aaacagttga
     2041 tatcttaggt gtagggataa tttcattttt cattgataaa atagttaata attcacttgg
     2101 gacagatttt acttatcttg attttattgg agatgagggg aaagttgcaa atggattttc
     2161 tgaaaaaatt attcaatttc aagaaaatag aagtaaactt aattaagaat cgtttactat
     2221 ttaaacagtg tccttttcct ttttaaatat atacgataaa ataagtatat gaattgtaaa
     2281 aggacatgat gattatggaa gaaatagaat ggaataatct gactcaaaat aataaaaata
     2341 attctggatg tggaattatc tttatgtttg ttatagtttt gggaatagta gtacttttaa
     2401 taactgtcaa gatagtagat tactttgcga atactcctga agaacaaata cagagaaaac
     2461 ttcctgagtt tgaaagagta ccaccttatg atccaagaga taattcattt caagtaaaaa
     2521 aattaaaaga gtctccaaat gaaatgtggt ttgaagttac agttgaatca agcgacttca
     2581 aagaaaaaaa gaaagaatac aa
//
LOCUS       DACDRN010000142         2589 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDRN010000142 DACDRN010000000
VERSION     DACDRN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.147"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..372
                     /locus_tag="IWJ80_002781"
     CDS             <1..372
                     /locus_tag="IWJ80_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HAP9462437.1"
                     /translation="FNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYISEDLWE
                     KLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRDMYKKY
                     TGKTGCLDSTYAADIEERREQ"
     gene            369..911
                     /gene="sat4"
                     /locus_tag="IWJ80_002782"
     CDS             369..911
                     /gene="sat4"
                     /locus_tag="IWJ80_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP9462438.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            1004..1798
                     /locus_tag="IWJ80_002783"
                     /note="aph(3')-IIIa"
     CDS             1004..1798
                     /locus_tag="IWJ80_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9462439.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(1906..2094)
                     /locus_tag="IWJ80_002784"
                     /pseudo
     CDS             complement(1906..2094)
                     /locus_tag="IWJ80_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2074..>2589
                     /locus_tag="IWJ80_002785"
     CDS             2074..>2589
                     /locus_tag="IWJ80_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9462440.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIE"
BASE COUNT          870 a          420 c          617 g          682 t
ORIGIN      
        1 tttaatcaga ttgttcgcca tgagctgctg agaatgatat catggaaggt cggcatcgaa
       61 acaggcttta aattaagtgt aggcaagaac tataagttta ttgaaaggta tatatccgag
      121 gatttgtggg agaaactttt gtccacctac cggatggatt cctatgaaaa catatgggaa
      181 gcattatttc tatgccatca attgttcagg gcggtatccg gtgaggtggc ggaaaggctt
      241 cattatgcct atccggagta tgataggaat ataacaaaat ataccaggga catgtataaa
      301 aaatacactg gtaaaaccgg ctgcctggat agcacatatg ccgctgatat agaagagagg
      361 cgggaacagt gattacagaa atgaaagcag ggcacctgaa agatatcgat aaacccagcg
      421 aaccatttga ggtgataggt aagattatac cgaggtatga aaacgagaat tggaccttta
      481 cagaattact ctatgaagcg ccatatttaa aaagctacca agacgaagag gatgaagagg
      541 atgaggaggc agattgcctt gaatatattg acaatactga taagataata tatctttact
      601 accaagacga taaatgcgtc ggaaaagtta aactgcgaaa aaattggaac cggtacgctt
      661 atatagaaga tatcgccgta tgtaaggatt tcagggggca aggcataggc agcgcgctta
      721 tcaatatatc tatagaatgg gcaaagcata aaaacttgca tggactaatg cttgaaaccc
      781 aggacaataa ccttatagct tgtaaattct atcataattg tggtttcaaa atcggctccg
      841 tcgatactat gttatacgcc aactttgaaa acaactttga aaaagctgtt ttctggtatt
      901 taaggtttta gaatgcaagg aacagtgaat tggagttcgt cttgttataa ttagcttctt
      961 ggggtatctt taaatactgt agaaaagagg aaggaaataa taaatggcta aaatgagaat
     1021 atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat
     1081 gtctcctgct aaggtatata agctggtggg agaaaatgaa aacctatatt taaaaatgac
     1141 ggacagccgg tataaaggga ccacctatga tgtggaacgg gaaaaggaca tgatgctatg
     1201 gctggaagga aagctgcctg ttccaaaggt cctgcacttt gaacggcatg atggctggag
     1261 caatctgctc atgagtgagg ccgatggcgt cctttgctcg gaagagtatg aagatgaaca
     1321 aagccctgaa aagattatcg agctgtatgc ggagtgcatc aggctctttc actccatcga
     1381 catatcggat tgtccctata cgaatagctt agacagccga ttagccgaat tggattactt
     1441 actgaataac gatctggccg atgtggattg cgaaaactgg gaagaagaca ctccatttaa
     1501 agatccgcgc gagctgtatg attttttaaa gacggaaaag cccgaagagg aacttgtctt
     1561 ttcccacggc gacctgggag acagcaacat ctttgtgaaa gatggcaaag taagtggctt
     1621 tattgatctt gggagaagcg gcagggcgga caagtggtat gacattgcct tctgcgtccg
     1681 gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag ctattttttg acttactggg
     1741 gatcaagcct gattgggaga aaataaaata ttatatttta ctggatgaat tgttttagta
     1801 cctagattta gatgtctaaa aagctttaac tacaagcttt ttagacatct aatcttttct
     1861 gaagtacatc cgcaactgtc catactctga tgttttatat cttttctaaa agttcgctag
     1921 atagagttct atattagaga tgtaaagagt tttggtgaag aggtttgtga gtatggcttt
     1981 tataaccagg gtgcaatgag aagcactaac agcagctagc ttaagaacgc ataggatata
     2041 ctttttatgg aagtcacaga ggaggaataa aatttgaaca ggattaacag agtaacctct
     2101 attcttattc agctgcaatc aaaaaaaata attcctgcca aagaaattgc acagcgattt
     2161 aatataagct taaggacagt ttacagagat atccggacac ttgaagaagc cggaatacca
     2221 attggatctg aggccggaaa aggatatttt cttgtagagg gcttcctact tccgccggta
     2281 atgtttacag cggcggaagt gggtgcattg attacagcag ggaaattttt aaattgccat
     2341 ggagatgaat catttataaa ggattttgat tcagctatgt ataaaatcaa atctattttg
     2401 aagcatggtg aaaaaaacta tgcacaggag ttggagaaca gtattaacgt gtacagcaca
     2461 tctggacaga aaaatacatt ggcggataac gttattgcag caattcagac tgcaatctgc
     2521 aataaaaggg taatatcaat tcaatatcct gcatcaggag ggcaagaacc ggaaagcaga
     2581 atgatagag
//
LOCUS       DACDRN010000143         2583 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDRN010000143 DACDRN010000000
VERSION     DACDRN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.148"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(332..1465)
                     /locus_tag="IWJ80_002786"
     CDS             complement(332..1465)
                     /locus_tag="IWJ80_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9462441.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(1456..2124)
                     /locus_tag="IWJ80_002787"
     CDS             complement(1456..2124)
                     /locus_tag="IWJ80_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9462442.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
BASE COUNT          785 a          551 c          414 g          833 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgttacggt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaagagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acatttttga
      121 tcctttttat ttgattaatt atatccagct ggataataat tttgggggca acatttctat
      181 cttactatta tcttcctaaa gtagtagact ttcttattgg gaaaaattac aacaaaaagg
      241 agtggcttag ttgacaactc ctttttgttt acaaatcaat tggatatcta taaattgtat
      301 tttctaacga attttataga catctgattt gtcaaattaa gctagacaat atgtctttat
      361 ctatgcagct caaaaatact tccaatggtg ttcgataatt taatgatttt cttggaatat
      421 ggtttctttt cgaggcaacg gattgaataa aggtttcatc cgtgtcccta aagtccattg
      481 ttttaggtaa gccatcctgc ttgagcccat gcactcggta ctgatgagaa aaaattgatg
      541 cctgtttctc ctgaggaatg ccaattccct gatcttttac ttcaataatc acgctaccct
      601 tttggtttga aatggtgaca attaccagtt ttccttcacc atatttgatt gcattgtcaa
      661 ctaaattcga aaccgcccga taaatccaac gatctcgtcc atgaatcagg gttggttgat
      721 cttcagtaaa cgcaaacaaa agattcggat aattcatttt gtaggcatca caaacttccg
      781 cacagatcag tgaaacgtct acgacttccg tgctaagatc ctctgagtta ttggataatg
      841 tcaaaatatc gtcaatactg tcggtcatcc gatctaaaag cattaaattt ccttgatcgg
      901 catgttgccg ttctatttcc agattcgtac gcaaaatagc gatggcattt ttctgctcgt
      961 gggtcagata actatttaat cgtcgataat cctccaaatg accatcgaac ttatccttta
     1021 gcttattaaa agccgcattt aatgcctcgt ccttcacagt atttttagga acttgatcaa
     1081 taaacgttaa atcacgcgta atcgctaaga tttgattact gtaaattttg cgtaaaacca
     1141 accaaagaat aaacgttaat aacatgacgg cagaacaaat aaccagcacc atcgctggta
     1201 aatatttaaa tagttcgtcc tggtaagctt tatctatcat cgaaatagtc ataaattgat
     1261 tgttggaaga tttcgaagct tccatctgcg tctctgcttg aaccgtctta ccctcttcca
     1321 taaaataaac atcttcacct aaggtagatg cgatactgat tccctccaaa ttatttctca
     1381 gaaagaagaa actcactaaa atcacgcctg tcaccaacag tgaaaaagcc agcatcgtta
     1441 acgtcatttt aaaatttaat agcatagctg gtaccccttt gctcgaatgg tttttaagac
     1501 ctctttttca gctgcttcag ccagtttctt ttttagacga gccagatgca ctcgcagaac
     1561 cgacgaaaaa ggatcaaaat cttcatcgta gacatgttca gaaatttcct cagacgaaac
     1621 aaccgctgga tgacgctgag cgatgcacag taagatatca aattccttgg gtttcaggac
     1681 cacctctgtc ttttgatagc tgacccttcg acctaatgga tcaatcttca aagcaccaat
     1741 tgaaatctgc ggcagcgcac gtccatgata acgacgaata accgcgcgta ttcgtgcttt
     1801 taattctagt aactcaaagg gtttgactaa ataatcatct gctccaaaat ccaagccttt
     1861 tgctcgttct tcaatctcat cacgtgcagt aataatgata actggacttt cgacttcttc
     1921 agcacggaga ctttgtaaaa cagataatcc atctttttta ggcagattta aatctagtag
     1981 tatcccatcg tatgaattga tgaaggcctt ctcttctcct gactcgccat cataagctac
     2041 atctacagaa atattctcct gctctaggcc cttttttacg gataaaacca gctcacgatt
     2101 gtcctcaacc accagtacac gcatgtatat tcactcctca tacggattta gtaattacac
     2161 ttatgctttt tagcatagta cacgctcttc ttcaaagtcc acgttctttt tgtgaagatt
     2221 tgaagaaatt catacattct gttaaaaaac acataattaa gaatgatttt actgtttgaa
     2281 aatacctaga atagaaaaag actggaacct ttcctccagt ctcactactt tctatttgct
     2341 tggttttctt gtatatactt ttcaaagttc gttggtttat gaccaataag cctcgttaga
     2401 atactgaaaa ttgccatcaa gtcattaagt accacatgta agaaaatctc tctaaagaaa
     2461 aaccccttcg ttttaactca cttactattt tagaattcgt tcccctaata atgcagcagg
     2521 ttctgttgcg aagtttgaaa atcaaacgcg ccctctctga actccaaata tcttaggctg
     2581 tta
//
LOCUS       DACDRN010000144         2480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDRN010000144 DACDRN010000000
VERSION     DACDRN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.149"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1290
                     /locus_tag="IWJ80_002788"
     CDS             <1..1290
                     /locus_tag="IWJ80_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAP9462443.1"
                     /translation="VKALMNLYKKTYEFIGIFIGIVGVLIIPLLPLFIKNSTDIPHMY
                     RYYVLFLLNSVLSYFFTYKRSLAIADQKNYLVAINDFAFLFFMNIVQVIALYFYSNFT
                     LFLIVQILFTLFSNINISRTVDKRYSYLKEKDVVSLDKQTKDEVKKNVIGNMSSKIGG
                     VIVMGTDNILISTFVNLTAVGIYSNYSLIVTSVQNLCKQITNSITASVGNFAVSNKKK
                     DSFELFKKHFFINHSLIFFSSILLLTLMNPFISLWVGAKYMLPMSTTILIVLNYSVQV
                     YRNTSFVFIESFGLYWSQRKKPIIEAGVNLLLSLLLLIVFDMGINGVLIGTIGSSLGF
                     VIWYEAFIIYKTVFEKRFIHFFLTFWRSFIELIISAIVVFLLNEYLANGGSTFLQFIT
                     KTLITIVTTSILYLLLYFRREEFTFIKKILFRYILKK"
     gene            1347..2462
                     /locus_tag="IWJ80_002789"
     CDS             1347..2462
                     /locus_tag="IWJ80_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9462444.1"
                     /translation="MGGASIVASKIAKGLIDSGHEVFFVSYLESEKPQVESKYYDLYE
                     KKIFLFKNFSRFHKILEYRLFGGFVPSRYIKKEKEQLKKVLKKEKPDVIIFNTFIPSV
                     LFSEFLKYEFPTVKLITWMHSDPDYSLNHIAKFYKKEYRKSFERVDSIVCLSQDTKEK
                     LSLYNNHIKVIYNPLTLSGSSLISNLDKKIISFTARYDINIKGFDYLCEVAKNLPVGW
                     AIRIAGDGTEEERKELKRLIEKNGVLDKIICAGALFGDELINHYIESSIFISTSRSEG
                     LPLVLIEAMTYGLPVISFDHGGAKEILQDGNYGILIKNFDVQDMNNEIEKLSNNRLLM
                     EKYQKFSLERAKDFNEKKIIGEWQTLLNELGNEDVRN"
BASE COUNT          894 a          284 c          400 g          902 t
ORIGIN      
        1 gtaaaggctt taatgaattt gtataagaag acatatgaat ttattggtat ttttatcggc
       61 atagtaggag tattgattat tccgctgtta cctctattta ttaaaaatag cacagacata
      121 ccacatatgt atcgatatta tgtattattt ttattaaatt cagttcttag ttattttttc
      181 acgtataaaa gatcacttgc aatagcagat caaaaaaatt atctggttgc gattaatgat
      241 tttgcctttt tattctttat gaacattgta caagtaattg ctttgtactt ttattctaat
      301 tttactctat ttctaatagt gcagattttg tttactctat tttctaatat aaatatttcc
      361 agaacagttg acaagagata ttcatattta aaagagaaag atgtagtttc tctagataag
      421 caaacaaaag atgaagtaaa gaaaaatgtc ataggtaata tgtcctcaaa aataggcggg
      481 gttattgtaa tggggacaga taatatatta atttctacat ttgttaattt aacagctgta
      541 ggaatttatt ctaactattc attgattgtc acaagtgtgc aaaatttatg taagcaaatt
      601 actaattcta tcacggcaag tgtaggaaat tttgctgtat caaataaaaa aaaggattca
      661 tttgagctat ttaaaaaaca tttttttatc aaccactcac taatcttttt ttcttctata
      721 ttgttattga ctttaatgaa tcccttcatt agcttgtggg taggagccaa atacatgcta
      781 cctatgtcaa cgactatttt aattgtttta aattatagtg tacaggttta tagaaataca
      841 tccttcgttt ttatagagtc atttggttta tattggtctc aacggaaaaa gcctataatt
      901 gaagcaggtg taaacttgtt attatctttg cttttgttaa tagtttttga tatgggaatc
      961 aatggagtac ttatagggac tatcggatcg tctctaggat ttgtgatttg gtatgaagca
     1021 tttatcatat ataaaacggt atttgagaaa cgttttattc atttcttttt aactttctgg
     1081 cgaagtttta tagaattaat aatttcggca atagtcgttt ttctattaaa tgaatattta
     1141 gcaaatggag gcagtacgtt ccttcaattt ataactaaaa ccttaataac tatagtaaca
     1201 acatcgatat tatatttgtt attatatttt agaagagaag aatttacatt tatcaaaaaa
     1261 attcttttta ggtatatttt aaaaaaatag ctttctataa aggagtgttt tatttgaaaa
     1321 tagctatact atctccagct ttctcaatgg gaggagctag tatcgtggcc tcaaaaattg
     1381 caaaaggatt aatagatagt ggccatgaag ttttttttgt ttcttactta gaaagtgaaa
     1441 aaccacaagt agaatctaag tattatgatt tatacgaaaa aaaaattttt ttatttaaaa
     1501 atttttctcg atttcataag atattagagt atagattgtt tggaggcttt gttcctagta
     1561 gatatataaa aaaagaaaaa gaacaactaa aaaaagttct aaagaaagaa aaacctgatg
     1621 tcatcatatt caatactttt atacctagtg tactattctc cgaattccta aagtatgaat
     1681 ttccaacagt gaaattaatt acttggatgc actctgatcc tgattacagc ttaaatcata
     1741 ttgctaaatt ttataaaaaa gaatatagaa aaagttttga acgcgtggat tccattgttt
     1801 gtctttcgca agatacaaaa gaaaaacttt cattatacaa taaccatatt aaagttattt
     1861 ataacccatt gacattaagc ggctctagtt taataagtaa ccttgataaa aaaattattt
     1921 catttactgc cagatacgat ataaatatta aagggtttga ttatttatgt gaagtagcta
     1981 aaaacttacc tgttggatgg gctataagga ttgctggaga tggtacagaa gaagaacgta
     2041 aagagctgaa aagattaatt gagaaaaatg gtgtgttaga taaaattatt tgcgcaggtg
     2101 cattatttgg agatgaatta atcaatcatt atatagaaag ttctattttc atatctactt
     2161 ctagaagtga gggactacca ttagtgttga tagaagcaat gacttatgga ttacctgtaa
     2221 taagttttga tcatggtggt gctaaggaaa tattacaaga tggaaattat ggaattttaa
     2281 ttaagaattt tgacgtacaa gatatgaata atgagataga aaaattgtct aataatcgtt
     2341 tacttatgga aaaatatcaa aaattctctt tagaaagagc taaggatttt aatgaaaaaa
     2401 aaataattgg tgaatggcaa acattgttaa atgaattagg aaatgaggat gttagaaatt
     2461 gaggacttcg attaaaaatt
//
LOCUS       DACDRN010000145         2419 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDRN010000145 DACDRN010000000
VERSION     DACDRN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.150"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            5..1150
                     /locus_tag="IWJ80_002790"
     CDS             5..1150
                     /locus_tag="IWJ80_002790"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP9462445.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKRDEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IWJ80_002791"
     CDS             1321..1779
                     /locus_tag="IWJ80_002791"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP9462446.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="IWJ80_002792"
     CDS             1938..2408
                     /locus_tag="IWJ80_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP9462447.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          809 a          454 c          455 g          701 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagatgaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaa
//
LOCUS       DACDRN010000146         2399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDRN010000146 DACDRN010000000
VERSION     DACDRN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.151"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            207..2069
                     /locus_tag="IWJ80_002793"
     CDS             207..2069
                     /locus_tag="IWJ80_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9462448.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2399
                     /locus_tag="IWJ80_002794"
     CDS             2069..>2399
                     /locus_tag="IWJ80_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9462449.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatt
//
LOCUS       DACDRN010000147         2383 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDRN010000147 DACDRN010000000
VERSION     DACDRN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.152"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1016..1306)
                     /locus_tag="IWJ80_002795"
     CDS             complement(1016..1306)
                     /locus_tag="IWJ80_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462450.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1514..2205)
                     /locus_tag="IWJ80_002796"
                     /pseudo
     CDS             complement(1514..2205)
                     /locus_tag="IWJ80_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          692 a          445 c          339 g          907 t
ORIGIN      
        1 acatacttgc aaagtaatac tacaattcct aacataatcg tagaatttaa taatttccat
       61 agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct tgaactccat
      121 gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct acttgcataa
      181 actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa gataccccaa
      241 atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt atgtttgttc
      301 atttttatac tccttcttat atttcctaat agagtataac atagctttaa gcattactta
      361 aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat ttgatgcaaa
      421 agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt tttcttagtt
      481 aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa gacatacaat
      541 catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa aatccaaatg
      601 ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac
      661 ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca ggaacgttta
      721 aaatcctaca attccactac ctgagactag taatgactag tctcttttta tggcctctga
      781 tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc tttaagcaat
      841 atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa tacatgtagt
      901 tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg
      961 catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt gttttctagt
     1021 ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca tattctacca
     1081 caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt ctattattat
     1141 aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag ttcaaactat
     1201 gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt ctggcactaa
     1261 cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc ttcacctcaa
     1321 taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta tctttttgac
     1381 tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat gaaggacatg
     1441 tactaaaaaa aagaggcagc agtatagatt actgttgctt ctttactaat ttaggtttac
     1501 ttgttaagaa agattattta cgcaaatgtt cttcaacaat ttgctgagat ttatcagctg
     1561 tatcttccac ttcatctttt gctttttcta acttttgaat atattgtttc aagcttgagt
     1621 tttcagcagt tgcttcgtct aatttctttt gtgtatctgt taactgattt ctacatttgt
     1681 tttttcttgt gtcagtgttg cgatttgttg attcaaacca tttacagtat tttgtaattc
     1741 tgaaagtttt gcttgtgctt cttgtagtct tgctgtccaa tctgcattca gatgattgat
     1801 ttcatcattt ttttggttga tctcatctag tttgacattg atttcatttt gtttgctttg
     1861 tagttgtgca tccttgctga ctaattgact ttctaagttt tgtttttctg ctgccgattg
     1921 atttttttgg ttttctaatt catttacttt tgtttggtat tcttggacat ctgcctttga
     1981 atcttgcaat gtctgttctt tttgtgctag tcgagcggta agatctgtaa tcgcttgctc
     2041 tttatctgtc aatgtatgat caagctgtgt cagattctca ttgattgtat caatatctgt
     2101 ctctccaccc cacaaaataa tttcatttgc gtaccgcgtg cctgtgtagc caattagaca
     2161 tagaaatgca acgacaatcc caccagtaat aaatttcttt ttcatttgca tagctcctta
     2221 atccaatact tttacatgct aaaagtaagt gatctcaaac acaatgtcaa gcgtttttaa
     2281 attacatatg taattttaat ataaatacta tcaaatactc atttaatcct ccttttttat
     2341 ctatttaaaa aatctttttt atagagaaaa atgcaggtta ata
//
LOCUS       DACDRN010000148         2377 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDRN010000148 DACDRN010000000
VERSION     DACDRN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.153"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            488..673
                     /locus_tag="IWJ80_002797"
                     /pseudo
     CDS             488..673
                     /locus_tag="IWJ80_002797"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            867..1037
                     /locus_tag="IWJ80_002798"
     CDS             867..1037
                     /locus_tag="IWJ80_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9462451.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1461..2327)
                     /locus_tag="IWJ80_002799"
     CDS             complement(1461..2327)
                     /locus_tag="IWJ80_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9462452.1"
                     /translation="MAIDGLAPGAYQLVETQAPTGYELDATPIEFEIERSQTAVVELT
                     KANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPG
                     VYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVL
                     QGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFK
                     IEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLV
                     FISIKFRKKRNI"
BASE COUNT          653 a          501 c          407 g          816 t
ORIGIN      
        1 gctgcttctc gtattttttg ttttaaaata aacggatcta gttgtagaga ctgataaaaa
       61 tccacctgtt gtcgtccttt ctttttactt ttatttttta cagtcatcta tgacaggttg
      121 taaatactat ggaacatcat atggcgttgt cattttcttg tcaactattt ttcctattca
      181 tgtttttata aaaaaacata tcgttttctt gtatgattaa atcaccacaa aataaacaac
      241 tgagagaacg atatgcttta tgaatggttc tatcaaaaaa attctgagat caatagacaa
      301 aaatttgatg attacagagg tctcttacga aaccttacag aagaaaaaaa cattgatcgt
      361 tgctgcagtt ctcttccctg ctccatgtgt ctgtagaaat tgtggttctg ctgtggtaga
      421 tggaaatagg aaattaatgg tggtgaaaaa caagaagaag gaaaccattg tccactttga
      481 ataatataat catatgccaa tggttatacg cctaaaaaag cagcgctata cctgtaaaaa
      541 ctgtcgaagc cattggaacg ctcaaagtta ttttatccgt ccaaggcact cgatttcaaa
      601 tcatgtcaga cataaaatca cttctttact gactgaaaaa gtgtcgttat tttttatttc
      661 gaaaagttgt taggtatctt tgacaaccgt tattcgcaca ttgaaagagt tgagaagcta
      721 tttaccaaag tcttccaaaa gaattctgtc ttgagtgttg agggttgtga atttcgttct
      781 cacgtttccg ttgaggataa actgagtttt atttccgcgg atggcgagac agaaaaatta
      841 atcgagaaaa tttacctaca caaaaaattc ctcgattaac aaactatttc cttagctgca
      901 ctaatcctaa agaaatagaa ttcttagtta cagacatgaa cgccacctac tttcagctca
      961 ccaaacatgt tctaccaaat gcgaaattgg tgattgatcg attacttggt ttttccacgc
     1021 ctttcaagga ggcgtaacct ttattcacga gttggttgaa gctttccgag acaaaagacc
     1081 cgacttgttt ttctccttat tgacaaagag ccattaaaac tagagaaaaa attacaactg
     1141 atcgctgatt atttgtagaa agaaaatcaa agtattaccc aataaacttg agtcattagg
     1201 caaaaaacac tgcgaaactc aagaatgatt ttcgcagtgt ttttgtatat tttttattaa
     1261 ctagtattac tacaaaggtt tatttttcac ttctcaacgc agcacaaaat tgtctttact
     1321 tatttactgg ttgggttcaa ccttctctac tccgatacta atatttgttt gtatacccct
     1381 tctaaaatcg gtagtttcca ttcaattgat gggtactagt tgattttaaa aactatgctt
     1441 attcaatttt ttattttagc tcatatattt cgtttctttc taaattttat actgatgaaa
     1501 acaagtagca cacccaatat tgacaatata aactggctaa tgatggtttc tccagttttt
     1561 ggaagatgct tatttccact gcttgttgga ttgtttcctc ttatatctga gctatcagca
     1621 acttttctat tctcttttgt taaaaagatc gccacttctt gttctttttc gatcttgaaa
     1681 gttactggtg tcgcatccaa ttcatacccc gttggcgctt gagtttctac cagctgatat
     1741 gctcctggtg ctaagccatc tatcgccagt tttccatctg ctcctgtggt tagacctgac
     1801 tgcagaacgg ttccattggc atcttgtaac tcaaaaacag ctccttgcag gacttctccg
     1861 ctttggtcat cgatctttgt caacacgaca ccgcctggtg taagtcgatt ttctttcgtc
     1921 aattctacca ccgctgtctg acttctttcg atctcaaact cgattggtgt cgcatccaat
     1981 tcataccccg ttggcgcttg ggtttctacc agctgataca ctcctggtgc taagccgtca
     2041 atcgccagtt ttccatctgc tcctgtggtt agacctgact gcagaacggt tccattggca
     2101 tcttgtaact caaaaacagc tccttgcagg acttccccgc tttggtcatc gatctttgtc
     2161 aacacgacac cgcctggtgt aagtcggttt gctttcgtca attctaccac cgctgtctga
     2221 cttctttcga tctcaaactc gatcggtgtc gcatctaatt cataccccgt tggcgcttga
     2281 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
     2341 cctgtagtta gaccggtttg tagcgtttct ccttctc
//
LOCUS       DACDRN010000149         2367 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDRN010000149 DACDRN010000000
VERSION     DACDRN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.154"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            152..1453
                     /locus_tag="IWJ80_002800"
     CDS             152..1453
                     /locus_tag="IWJ80_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9462453.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(1557..1757)
                     /locus_tag="IWJ80_002801"
     CDS             complement(1557..1757)
                     /locus_tag="IWJ80_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9462454.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          768 a          454 c          408 g          737 t
ORIGIN      
        1 ttcgtacaaa aaaaccgtct tttcttggct ctataatttc taggactgat agaaaatcat
       61 tctatcggtc ccttttgagc atgaaaaaac aaaacataca gaagctccgt tagaattgaa
      121 tcaccacaac acaatcaaag gagcgttctg tatgcctgat cttcatcata tcaaactact
      181 acttggaatc aaagataaaa acattttcat cacaaatgtg gaatcgaaga gcattaagaa
      241 tatcaagagt cttgttgttt ctgcgacgtt aagtaaagaa attcgtcgct gtcctttgtg
      301 taaacagatg aatcatgaag gcatgatcgt taagaacgga aagaaaaaat cgctcattca
      361 acttaataaa tgtgcgaacc aactgactta tcttgcttta gcgaaacaac gctaccactg
      421 tcgtggctgc cacacctatt ttacggcgaa tacctacatt gtcgatagaa actgctttat
      481 cgctaaacaa gtgcgctata aaattttaga agagctaact gaaaaacaag caatgacgac
      541 aattgctaag cattgcggcg tttcttggtc gaccgtttct cggacactcg cttctctgat
      601 ccccatgaca aaagtaaaaa gaaattggtt accgcgttgt ttattaatgg atgaatttcg
      661 ttcactgaaa aatcaagtcg gtccctactc cttttcttgt atggatggcg atactggaaa
      721 attactggat attcttcctt cacggaagaa aaaggctctc gtttcttatt ttatgcaatt
      781 tgaacgacgc gcacgattga aggtaaaggt catcgtgacg gacatgaatg tttcttatgc
      841 ctctctgata aaggaatgtt ttccaaaggc aaaactcgtt gtggatcgtt ttcacattgt
      901 caaacatctc attcgtaaat ttgaggatat tcgcgtacga atcatgaaaa gctttgatcg
      961 aaacgatcca acacaagcca aatattatcg acaattaaag gcgttgtctc ggcttctgat
     1021 caaacgacag gacaagcttg tttatgacaa atggaccaaa tggcgcaatt ttggttgggc
     1081 gtatctcacc gaaagtgaag tggtggatcg tctgctttcg atctctgacg aattacgaat
     1141 cgcctatttt tactatcaag aaattcttca agcgtttcac gacaaagagg cggacacttt
     1201 ctttaaactt gtaagaacga tgcccaattc cgttccaaaa gaactgcatt ctataaaaaa
     1261 agcttttata aactatgaat ccggcattcg tttagccctc gaattgcctt attcaaatgc
     1321 caaaatagaa aatctgcata cccacatcaa agcattgaaa cgagtcgctt atggctttcg
     1381 aagctttcgg aaaatgaaga ccagaatttt cttactaaat aatctgatta cctatgaatc
     1441 aaaaaacatc taaagtagaa aaggaattct accttagatg ttcttatctc actctatcag
     1501 tcctatttga caaagagccc ttttctttcg aaaagacggt taaaagtcaa tttttattaa
     1561 gctttaacaa tgtttgttgc ttgagggcca cgttgaccat cttcaacatc gtaagacact
     1621 gcttggcctt cttctaaagt tttgaagccg tcaccttgga tagctgaaaa atgtgcaaat
     1681 acatcatttc catcttcacc agtgataaag ccaaaacctt tgtctgcgtt gaaccatttt
     1741 actgtaccgt tattcataaa taaattcctc ctgatacgta tcaatgtacg ttttttcgtt
     1801 gcaataaata tttgataggc aaaaggagtt ttatctatgt agatatatcg ctcaaattac
     1861 gtttcaaaat agattactca tttaatatat catgcttttt ctaataacac aaggataatg
     1921 caatgaaata taaaattcat tgtcttctat tgtcttcgaa tcgaagaaaa agttgaataa
     1981 ccgcgattga ttcacttttt gttttcttgt ttatttgctt ttatctgcaa cttttgactt
     2041 taaaatttct taactatgaa aacctttcaa ttggtggtat acttataaat ggaatttcgt
     2101 tatcaacgcg ttcgttgcct ttcagcaagt atttaaagaa aggagaatgg ctatgacatt
     2161 ctataacaaa gatatacaat atcgagtaac cttggataca gatttgaatc tatttgtcgt
     2221 ttttgacaaa gaaaatacca atcatgtagc gacaggtgtt acgatcgaac aagcagtgca
     2281 agaattaaaa aaatctgcat aaggagtgga aaccctcata tggacgattc tcgttcttct
     2341 ctttaaacgc atctactggt agataac
//
LOCUS       DACDRN010000150         2351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDRN010000150 DACDRN010000000
VERSION     DACDRN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.155"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..544
                     /locus_tag="IWJ80_002802"
     CDS             <1..544
                     /locus_tag="IWJ80_002802"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9462455.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1017..1589)
                     /locus_tag="IWJ80_002803"
     CDS             complement(1017..1589)
                     /locus_tag="IWJ80_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9462456.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1605..2210)
                     /locus_tag="IWJ80_002804"
     CDS             complement(1605..2210)
                     /locus_tag="IWJ80_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP9462457.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          740 a          469 c          359 g          783 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa tatcacaata
      601 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
      661 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
      721 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
      781 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
      841 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
      901 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
      961 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1021 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1081 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1141 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1201 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1261 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     1321 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     1381 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     1441 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     1501 ttctctacgc cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     1561 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     1621 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     1681 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     1741 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     1801 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     1861 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     1921 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     1981 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2041 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2101 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2161 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2221 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     2281 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     2341 gcacagtcta g
//
LOCUS       DACDRN010000151         2328 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDRN010000151 DACDRN010000000
VERSION     DACDRN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.157"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..332)
                     /locus_tag="IWJ80_002805"
     CDS             complement(<1..332)
                     /locus_tag="IWJ80_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9462458.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTK"
     gene            complement(663..896)
                     /locus_tag="IWJ80_002806"
     CDS             complement(663..896)
                     /locus_tag="IWJ80_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP9462459.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(911..1861)
                     /locus_tag="IWJ80_002807"
     CDS             complement(911..1861)
                     /locus_tag="IWJ80_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9462460.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1858..>2328)
                     /locus_tag="IWJ80_002808"
     CDS             complement(1858..>2328)
                     /locus_tag="IWJ80_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9462461.1"
                     /translation="FGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLITSFDPLMAK
                     AMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANSLKTMIFIS
                     ATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKKERKL"
BASE COUNT          741 a          433 c          292 g          862 t
ORIGIN      
        1 tatttggttc cagctgattg gaataaaacc aaatcgaata tacgtttatt ctcaaaaata
       61 taattaataa atggatctaa ttcattgaag ttctctctcc aacttttctc tatatctata
      121 ttttctaagt ttaagaggta tttggtaaac tcttcattat acatattaat aagcgcatta
      181 atatgcggct gaataatttc ttcaaataac gcttccttag aagtaaaatg tttataaaaa
      241 gcacccgtag ttacgcctgc ggtcaagcac agttgcctta tcttagtttg ctgaaatcct
      301 actttttcaa aatggcttag tccactttct attaattttt ggtgagttgt aatataatct
      361 ctggtttcta tgggtacttc ctcctaataa gatgaattaa ctatattatc ttacacagga
      421 cttgaagttt caatcgttaa cgtctattac tgaactcatt ccttagagct aaactaatga
      481 gaaagagcaa aataactgat acaaaaagca atcctacaag aattaataga ctcatctaaa
      541 tcattcacct tttctaaaat aatccgagaa agatttaaaa aaattatatc cagctattag
      601 gataagtcct atcataatca ctccaatagc cattttctta tcctccctaa aaataaagcc
      661 ttttacttaa aacctttttt gtaatcgtca ataaattttt tccctttatc agttaattga
      721 aatcctatat ctaaatagtc ctcatatcta agtttagtca gcatctcact tacagatgct
      781 ggactaacac ttagaacttt tgctaaatca ttatttttta atttcttttt tctttttgat
      841 aattcctgaa ttgctagtaa ataaattagt atagtagttg ttttaatttg taccatacct
      901 accctccaaa ttatttcgaa aggccttcag caattttatc caggttccat ttcatcatcg
      961 catagtaact atcaccatct tgtccttttt ctgcaattga atcagtaaaa atcgttgaat
     1021 agataggaat attggtatct tttgatactg ttttcattgg tctatcgtcc acactgcttt
     1081 ctacgaataa ggagggaact tttgttgtgc gtaatttttc aactaagtgt tttatttgat
     1141 ctggtgttcc ttcttcttca gtgttgattt cccaaatgta agcagaaggc acattatacg
     1201 ctttcgagaa atatttaaag catccttcac ttgtaacaat cattttttta tcattcggaa
     1261 ttgaagcaaa tttagattta gcttctttgt ctagagaatc caatttttca atatacttat
     1321 ctagattttc tttatagaat tttttattat caggatcttt ttccgctaac tgtttttcaa
     1381 tatttttagc gtaaataata ccgttttcta aatttaacca agcatgggga tcttccttcc
     1441 ctttctcact ctgaccctct aagtaaataa catctatgcc atcacttgct gcaaaatagt
     1501 ctttgttttc ctctttgttc gcatttttta ctaattttgt aaaccaagca tttcctccag
     1561 tttccaagtt aacaccgtta taaaaaatca aatctgcttt tgaagttttt tgaacatctt
     1621 caggcaaagg ttcatattcg tggggatctt tcccaatagg tacgatactg tgtaaatcaa
     1681 ttttatcttt tgctatattt tcagtaatat ctgctaaaat cgaattagta actactacct
     1741 ttaatttttc gttcgaagcc actgtatctt tgtctttact atttgttgaa catgccccga
     1801 atataaaaag agcaatcaaa atccccagac ttcccagtac tgctttccat tttctcatca
     1861 taactttctc tccttctttc ttaaaaattt ttgttttggt gagattagaa aactaattac
     1921 aaataagaat gccgctgtta aaacaatact tgatcctgct gctatgttaa aactataacc
     1981 aatgaacaaa cctagcaatg atgagcacgc tccgactgtc gccgaaataa atatcatagt
     2041 ttttaaactg tttgcatata aatatgctgt agcagctggt gtaattaaca ttgcaacaat
     2101 taatatagtt cccacacttt gcatagctgt tactgctact aaagttaata gaatcattaa
     2161 tagataatga taaaaactga cattcatccc cattgctttt gccattaatg gatcaaaaga
     2221 agtgattaat aattctttaa aaaatagaac aactgtaacc aacactaaaa cagaaacgcc
     2281 aattgtaatc cacttatcaa tatcttgtac agctaaaata tttccaaa
//
LOCUS       DACDRN010000152         2307 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDRN010000152 DACDRN010000000
VERSION     DACDRN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.158"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(135..425)
                     /locus_tag="IWJ80_002809"
     CDS             complement(135..425)
                     /locus_tag="IWJ80_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462462.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(543..803)
                     /locus_tag="IWJ80_002810"
     CDS             complement(543..803)
                     /locus_tag="IWJ80_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462463.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(940..1353)
                     /locus_tag="IWJ80_002811"
     CDS             complement(940..1353)
                     /locus_tag="IWJ80_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP9462464.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1809..2294)
                     /locus_tag="IWJ80_002812"
     CDS             complement(1809..2294)
                     /locus_tag="IWJ80_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462465.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
BASE COUNT          843 a          376 c          366 g          722 t
ORIGIN      
        1 gttactatga ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt
       61 caaaaaagat aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa
      121 tacattttaa tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga
      181 gagatcataa gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc
      241 catatttctt caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc
      301 cagcaccatt tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga
      361 agtaaaaaca aagctttact cataactaac aaaataccag ctccttttat ttttttctcc
      421 cccacattca aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta
      481 tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa
      541 gtttattttt ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag
      601 ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta
      661 tggataaaat agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat
      721 aagtaaaaga aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag
      781 acaatcaaat gatcaactcc catatttttt accaagagag atcataacat ctttatcgta
      841 aaaacaaaat atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata
      901 tataaaaatc gcccctcatt catcgagagg cgataagttc taatttaact caattttttt
      961 tgtttctttt ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa
     1021 ggttccagtg attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac
     1081 cgaatgtctt tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg
     1141 attggcggag atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca
     1201 gccaggcata tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg
     1261 ataacttgaa acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg
     1321 gaacatgtcg cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt
     1381 tttatttatc cctatttacg taataactct attttgaaaa aaatgcaagt ataatggtta
     1441 gctttgtgaa tatttcatct aatacgatac aaaggctatc tttctaattt gaaaaaggga
     1501 tcattaaaat aataagaaga aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa
     1561 atatagaaaa attgaatcta gaaaaccgcc cgacaataaa tttcattaac tcagttgacc
     1621 gaaaatgaag tagataccaa aaatttatca agagagcaag tagagaagtg agtatattca
     1681 taagagaaat agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat
     1741 aactttctca gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata
     1801 tcatctaatc aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac
     1861 actcgataaa aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct
     1921 gtgaacaaat aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt
     1981 aattcggaac ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga
     2041 gaaggtacag tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt
     2101 gattggctat gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa
     2161 gtacttttag ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa
     2221 ttctttgttt tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa
     2281 atccctttat gcatgatagc tcctcct
//
LOCUS       DACDRN010000153         2272 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDRN010000153 DACDRN010000000
VERSION     DACDRN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.159"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            225..479
                     /locus_tag="IWJ80_002813"
     CDS             225..479
                     /locus_tag="IWJ80_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9462466.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            480..797
                     /locus_tag="IWJ80_002814"
     CDS             480..797
                     /locus_tag="IWJ80_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9462467.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1356..>2272
                     /locus_tag="IWJ80_002815"
     CDS             1356..>2272
                     /locus_tag="IWJ80_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462468.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKG"
BASE COUNT          824 a          307 c          431 g          710 t
ORIGIN      
        1 aggatgggcg acttctgcct aattctgtta aatggatgtt aacggaagtg tatggtgagt
       61 agttcactcg tattttgata tcgtttgtat atgttttcag cgttctaagt ggcttatttt
      121 gtccaataat aatatggagt ggaatccctt aagaaacacc gttggcaatt gttgttataa
      181 catgttataa tttattcatc aaaaactaaa aggagtgtga taacatggga aaagaactaa
      241 aggttagaaa gatcggcaat agcgtgggag tgatccttcc aagctctctt ggattaaaaa
      301 gcggagacat tatccaagcc aaacaagaag gcaacttatt catattagat actactcaaa
      361 tagcaaaaga acacgatcgc aaattgattg aagaaagttt ccaagacttt gagaaaggtt
      421 taacagtttc agaaattgaa atggtaaaag ctttcggaaa atacggctgg agtgaataga
      481 tgaaagagta cacgctagtt tattctaaaa gttttcagac cagccttgaa acgataatag
      541 aagaatggga aaatgagctt gctctttctc cagccagcat aaaaagattt gtaggtgcga
      601 tccagaaggc tttagaacta accaaaaatt ttccaactat gcatgaggaa gtttccgaaa
      661 tctatggaat ggatatccca acatatcgga tcgtgatcgg caaaagctat gccttatttt
      721 atcggatcga ccatgaaaaa aaagagatac ttgtaggacg tattttccaa tctaaacaaa
      781 tgaaactagg cttttaacaa taattaggaa aaagtcgctc atcctctaca ggtgggtgat
      841 taatgccgct cgatatggtt gttaaccgtc ggttactcga agattgatat ataagactta
      901 gtaccttttt attttgctcc atgttatggc cgaaggtcta aagatacaat cattagtcta
      961 gagaaaaatt aagaaccgta ggaactacgg ggatcgcttg gtgaataaga gacactgcta
     1021 ttagtgaaaa aataagatat caagtacgct ctattcccaa aaatttcgtg actttactca
     1081 tgagaggttc aagtagtata tttttaatca aaattgacgt aacttttaat aaatactacc
     1141 taaataaggt agtatttatt tttgtgtttt aaaagaggaa gtccaatata tctatgggaa
     1201 acttcttaaa tattatgttg gtacatgaaa atatacatcc ctgctagact atttacaaca
     1261 ttttactttg tacgtattct tgttttaaaa ggaatgggat aaaatatagg tacaataact
     1321 atttgatgct aaatataatt aatggaggag agatagtgaa catacttaat gaaaaaagat
     1381 atgtatggtt tgctggtaaa aaatatatta ctactttcaa aagattatcc gagttaatgc
     1441 agcagactgt taaaagtgct atgcctgaag gatacaagtt taattttgtt caatcttcag
     1501 tatctactga aagtatctat ttttcaattc tttctgagag taggcttttt cattttagaa
     1561 taagttctca tgtaaagaaa actgatgttg atatatatac gttccgtaca aataattata
     1621 aaaatttaaa tgatttgagt agaagtatca gtgaatatct caaggggaat agctatctca
     1681 agattcgatt ttcacatata ttaattttac taccatattt gaatgagaat ttcaccctaa
     1741 ttcaagtaga taagtctttc attgcctcag gtaatcaagg aaatatgaat attacaagta
     1801 acaaaacgtc acgtgtgcta ttggcgttga tgggttacgg aattctttat agagatgacc
     1861 aaaatggcca gattctaata aatcgggctg ggcgttcatt attaatgaaa tatagaaaat
     1921 ttataccgga agattttcaa aacttacagt tagaaaacat gcgtttagaa gatttattta
     1981 gattagcaga agatgataag tatagatttg aagttttgaa aattcaaaag aatgaactat
     2041 tgaataagtt atatcctgag aatgcgataa aagcacattt tggtgatgct aatcatatgt
     2101 atagttggtt cattcctaaa agatttagag ggaaaatcaa tgctggagat caagtgaaag
     2161 tactcaatcg aaatagtaca gatgtcgtaa tgataacaga tatctatgta gaagatgaag
     2221 atataatcaa atatatgaag cctgtgataa aacttgttaa taaaaataaa gg
//
LOCUS       DACDRN010000154         2084 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDRN010000154 DACDRN010000000
VERSION     DACDRN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.161"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..621
                     /locus_tag="IWJ80_002816"
     CDS             <1..621
                     /locus_tag="IWJ80_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9462469.1"
                     /translation="FVKGDKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISGI
                     YSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFI
                     SIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLS
                     SLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNPKELLIDNN"
     gene            complement(907..>2084)
                     /locus_tag="IWJ80_002817"
     CDS             complement(907..>2084)
                     /locus_tag="IWJ80_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9462470.1"
                     /translation="IKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTL
                     DLEKEVKLKERKISKADPESGYYVKGEREKQFAYSVHTSCDDNGFILSTIITPRNIHD
                     SQVAFQLVKQSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLS
                     KNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNCPLLNQCTISKNHQR
                     VITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKV
                     ATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCD
                     VGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIVMFLWLFRMGVIP"
BASE COUNT          644 a          356 c          382 g          702 t
ORIGIN      
        1 tttgtaaaag gtgataagaa tcttgatagt gtaataaaag atcttgggaa attagatata
       61 aattggagag aatataattt gattaaaagt tcatcaaatt accctgcatt acaacaatct
      121 atatcaggta tttactcaat ttcaaataaa ttattttttg gctcattaat atttgcagga
      181 gttgtagttt cattattatt attcttatgg atgaatgcta gaaagaaaga aatagcagta
      241 ttactatcat taggtatatc aaaattagaa atttttggtc aattcctaat tgagatggta
      301 tttatatcaa tccccgcatt cgttggttcc tatttcttag ctcaatatac agctgacaag
      361 cttgggaata atatattgaa taaggttact ggtgatatag ctaaacaaat agctaaacaa
      421 tctgcatcta gccaattagg tggtggggca gaagctgaag gatttaacaa gacattatca
      481 agtttagata tcaatgtatt acctaaattc atgatttatg tagttatatt tatgagttta
      541 gtacttcttg tatcattgat tatttcttca ttcaatatat taagaaggaa tccaaaagaa
      601 ttattaattg acaataatta aaattttcgg tgctttttag caccaaaaat gagagtgtaa
      661 tataaaccgt gtaagtaacc atcaatccta ggcgggtggt tgcttcacgg tttttatttt
      721 tctaaaataa gatcaagagg tgattacacg atggctagaa agaaaagaaa tcctgatgct
      781 gagaagttag ctgaatccat tttgaatact tatcaacccg aatctgtaga ggatatgcaa
      841 gatgttttaa aagatgtgtt tggtccctta tttgaaaaaa tgcttcaagg agaattgaat
      901 aaccatttag ggtatgacgc ccattctaaa gagccaaaga aacatgacaa tcgacgaaac
      961 ggttatggca ataagacgct taaaacgagc tttggtaaag taaacattga tgttcccaga
     1021 gaccgggagg cttcttttga acctgagttg attcctaata gaaaacgaga tgtctccgac
     1081 atcgcagact gtagacaaaa gctgttccaa actcgtaact cgagcttgga acagcttttt
     1141 atctacttcg tttcgaattt ttgaacaaaa aaataaaggc tccttgcctt tccatagcca
     1201 ggtggctaat ttctttaaat tcattgcagc aaacactagc atcgtatgcg tcgcgacttt
     1261 ttccaagcct cgatacttgg tccaacgcat cccatgcttt tcttttgcat ccgcaaatat
     1321 tcgttcaatc gtttgtttcc gttttttgta aattgattta ttcagatctg ttaggcgaag
     1381 atgttccact tcatccatca gatctcccca aacatgtctc gtgatgactc gttgatgatt
     1441 tttacttatg gtacattggt ttagcagagg acagttcaca cattcttttg gattggattt
     1501 gtattctcgg tacccttctc gagtaaccgt tgaaaaagcg agcatttgat cttgcggaca
     1561 aatataacaa tcaaaatact cgtcatagag aaactcattt tttgataata gctccttttt
     1621 ccctttgggc cttgaataag gcaaacaagg tcgaaggttc aaatgagtca aaaagtgaac
     1681 aaacttaggt gtcttatacc cagcatcagc gacaacacaa ttgatttcag gaaaaagtct
     1741 cttcgattgt ttgacgagct gaaaggcgac ttgactgtca tgaatatttc taggagtgat
     1801 gattgtgcta agaataaatc cattgtcgtc gcatgaagta tggacagaat aagcaaattg
     1861 tttttctcgt tcgcctttga cgtagtagcc gctttccgga tcagctttac ttatttttct
     1921 ttctttcagc ttcacttcct tttctaagtc caaagtaaaa gggcgttttc cagcctctat
     1981 tcgttgacga ttgatctcat cttctaattc atcctgatag gctttcgctt catcatgggt
     2041 catttctttc gtaaacttat gtttattggc gttagctttg atgt
//
LOCUS       DACDRN010000155         2053 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDRN010000155 DACDRN010000000
VERSION     DACDRN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.162"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..120)
                     /locus_tag="IWJ80_002818"
     CDS             complement(<1..120)
                     /locus_tag="IWJ80_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9462471.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIY"
     gene            475..831
                     /locus_tag="IWJ80_002819"
     CDS             475..831
                     /locus_tag="IWJ80_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9462472.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            841..1740
                     /locus_tag="IWJ80_002820"
     CDS             841..1740
                     /locus_tag="IWJ80_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP9462473.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          692 a          333 c          398 g          630 t
ORIGIN      
        1 ataaatatcc aatgctttgg ttttatcttc attgacaaag agtgttttta cttcaatata
       61 agaaccctcg atattttgtg tttcttcgat aaaatagatg ttttttatat attccttcat
      121 agtgattcct ccaaagaagt cttttatttg ataagtttac tctaccgaag agttgttaat
      181 aatccgtgaa caaaaagtaa ttctttttcc aatagttgtt aaaggttttt tattttttat
      241 agtatttttt gttatttaag aaaggaataa ggttaaacaa ataacaggtt gctttttggg
      301 tctttttccg ttcaagaaac tgaacgaaga aaagggaaag ttgtctcttt tgtgataaga
      361 gaaatcataa taaacgaatg acttactttc caataaattg agaacagtca gaaccagaca
      421 aattagttgc atctgatgaa taaagtgatt attctatgac atgaggtgat acagatgaaa
      481 ttagaagtga caaaagaagc acaagaagta ttgaagaata aattagaagc agacgatcaa
      541 cttttattag atatcgaaaa cggtgacggt ccatttgcag aaagccaagt cagttgccaa
      601 ttggatacgg catttcgatt gatcattgtc aaaaaagata cacaaacaga tctaagtctt
      661 tattctgtag tggctgatac accagttgga ccaatcaaaa tcaaagacag tgcaatactt
      721 tacatggatg atccatccac acttgcttta gaaccaactt ataatagttt acaattaaaa
      781 ggaccaagcg gcttgagaaa aggcaacttg caagttgttc gaaaagacta gagggataaa
      841 atgggaaaat tggcgatcat cagcgaccta catgtggaca tcaataaatt cggcgaatca
      901 gagctgatgc agctttggca agttttgcaa aagcacaaga tcactcggct tcatctagcc
      961 ggagatacgg ctaataaagt agaccgttgc ctagctgttg tcgaattttt cgtcaataaa
     1021 ggattatcta cgacatttca tttcggcaat cacgagttgg cagatgtgcc caatgaacag
     1081 atgatggacc attttccaga cccacatttt ttaaatgaac ggtatattgc tttaaatgag
     1141 cagaccgttc ttttagggat gaatggctgg tacgactatc agttttccga attgaaagat
     1201 tacgaggcaa tcgtacgaat gaagcgtttg tattggtatg atcggttcat ccagcgtggg
     1261 aaaacagatc tcgaggtcaa tgcagaaatg cttgcagctg caaaaaattt gctagaccag
     1321 ctggaagcaa aaaaacttga ggtgatttta tccactcatt tcgttcctaa acgggcattt
     1381 atcatgtatc aatctgctcc ttatgagcgt tggaacaaat taaatgcatt tttaggatca
     1441 gaatcattcg gagaattact tgaccgttat tcaaatatca aacaagtagt ttttggccat
     1501 acgcatcgtc gctttgaaga taaatccatc ggtcacacga tttacagctg cagacctttt
     1561 ggttattatt atgaatggca gctgacccga gattttgtct tgaaagaaca gttgctagac
     1621 agctatgatc ctatgaaatt acgtgtcctc ttgcgtgccc atcacccagc ttttcaggcc
     1681 tatcaaacac aacatttgca agaggaattt gaacaagcga tgacaatcgt tccgtattga
     1741 gacagcatta gtaatgtgta aaagaaaata ccttgtaaaa cataaggata agtaagagca
     1801 gtagatccat ttatcgtgga tatgctgctt tttttgcgga aaataatcag cgaatgttta
     1861 tttgatcgga tgatttgtaa ctcgcagagt actagatgca agcgtgtatt ctttttttga
     1921 acgataatca caaaaaaaat ataatataca ttttttattt aattataatt aatattgatt
     1981 gaagtctagg cagtcaagtc gagagtgtaa aatattttgt gtaaatgaaa aaatccatac
     2041 aaaaaaggaa gtc
//
LOCUS       DACDRN010000156         2023 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDRN010000156 DACDRN010000000
VERSION     DACDRN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.163"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..566
                     /locus_tag="IWJ80_002821"
     CDS             <1..566
                     /locus_tag="IWJ80_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462474.1"
                     /translation="EQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEK
                     YIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDD
                     YYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYT
                     YDDTEEIQNELDRKRMPYQRFERKKRQ"
     gene            947..1126
                     /locus_tag="IWJ80_002822"
     CDS             947..1126
                     /locus_tag="IWJ80_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462475.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            1257..1526
                     /locus_tag="IWJ80_002823"
     CDS             1257..1526
                     /locus_tag="IWJ80_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9462476.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            1519..1776
                     /locus_tag="IWJ80_002824"
     CDS             1519..1776
                     /locus_tag="IWJ80_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9462477.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          739 a          289 c          412 g          583 t
ORIGIN      
        1 aagaacaatt gtatttggaa agtgaaacga gtcaaacgaa ggtcacagtc agccataatc
       61 gtttagacgt gtataaacgt cgtcctgaaa cggtaaaaaa aggaacggag cttctacgac
      121 tagaaacaga aaaatacatc atgaatgttt acctagaaag gagtgagctg gtcgaaaact
      181 ttgtcagcta cacgatttgc aggaaacact attactcaga atcagaatat tattcaattt
      241 ttgagagcat ggaattaatg gaagaagaaa tggaagagct aaacagcaaa gatgcgacga
      301 tagacgatta ttaccttgta tttactagat ttttgacgtg tggaacggaa tctatcgtgt
      361 taatgggaat ggattatgaa ctcgctgtac ccgaagcatt tttgagggaa tatgtagaag
      421 aagtagataa cccaggcgac gactggtaca cgactttaac tgatttttta gacacctata
      481 cttacgacga tacagaagaa atccaaaatg aacttgaccg taaacgaatg ccatatcaac
      541 gatttgaaag gaaaaaaagg caataatcat ttataccgaa aagtgagaat gtgtgaaata
      601 aggacaaaaa acctagtaac taagggaatg gttcttattt cgcaccaatc gctccaaccg
      661 cccccgccgc ttaggtggtg cgaaataagc gggcaacacc cgataaccta agagaaagaa
      721 ggagatccat atgaataaaa tcattcctct attgttagtc ctatttgttg gcgaaatact
      781 tatgcgccat gctgcagctg tgcatttaaa cttcgtagta tttttggcaa tactggtatt
      841 ttgtttgatc ttttggcgct ttgtattagc ccttgtgagt ctactattca agatgattgc
      901 agccatattt ttcatagggt tatttcgatg aaaggagaga gtactaatgc caattgcaat
      961 aggaaataaa cggttacccg ttacgttgga tgaaaagaga caaaaagaat tgcagcaact
     1021 aaagcagaag tacggcaaaa gtgaatccag gattatgtgt attgcgttag atttattgat
     1081 tgcccaagaa aaagcaggat ttgaggtacc agcactcaaa aagtgacgtc actttttatc
     1141 ctaaaaacta aaagtgatag cacttttaat tataagaagt tagaatatta atcatttgct
     1201 taattgtaca atataatgta caattgtttt atagaaataa ataaggggtg aaaggaatgg
     1261 aagcagtagc ttattcaaat ttccgccaaa atttacgtag ttatatgaaa caagttaatg
     1321 aggatgctga aacacttatt gtaacaagta aagatgtaga agatacagtt gttgtattat
     1381 caaaaagaga ttatgattct atgcaagaaa cgttgagaac actttctaat aattacgtca
     1441 tggaaaaaat tcgtcgagga gatgaacaat tctccaaagg tgcatttaaa acacatgact
     1501 taatcgaggt tgaatctgat gattaaggct tggtctgatg atgcttggga tgattatctt
     1561 tattggcatg agcaaggaaa caaaagcaat ataaaaaaga ttaacaagtt aataaaagat
     1621 atcgatcgtt ccccctttgc tggattagga aaacctgagc cattaaagca tgatttatct
     1681 ggaaaatggt ccagaagaat tacagatgaa catagactga tatatagagt tgaaaatgaa
     1741 acgatattta tttattctgc aaaagatcac tattaaccaa tcggaagtaa ggaaagggtc
     1801 agaaacttaa aagtttttga tccttatttt atttacccta gtcatttaaa aagctaatat
     1861 agcttagtgt tgattgttat taatgaatgt gtttgttacg cgtattacgg atataaggtt
     1921 agtaaaatca tttctaaagt tgaggaaaag taaatataaa tggcttaaat ttcaacaatt
     1981 tgaagttgaa tagatatgtt ataatactat tgtagtgtgg gat
//
LOCUS       DACDRN010000157         1959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDRN010000157 DACDRN010000000
VERSION     DACDRN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.164"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..708
                     /locus_tag="IWJ80_002825"
     CDS             <1..708
                     /locus_tag="IWJ80_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9462478.1"
                     /translation="FVDSDDYIDEDMYEILYENLKIHDADISSVELIPFYGDRYKKAN
                     KEKKVIILNKKEAIKSVLEGTQFYAYAWNKLYRKELFKDNRYLDGKTFEDAYIIIDLL
                     FQTEKIVVSNEEKYFYLQRNDSIMGKSFSMNNFDVIEAWQYNKEKILDAFPDLHDSYY
                     RRLCWAYFYVLDKMVLSSSYQQIPRKKTVIEFLKKNRNFILTYKGFTKSRKIAIIALS
                     FRVDWYRKLVEQKNKKN"
     gene            753..1727
                     /locus_tag="IWJ80_002826"
     CDS             753..1727
                     /locus_tag="IWJ80_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9462479.1"
                     /translation="MCRISIIVPVYNVEKYLKKCVDSILGQTFTDFELLLIDDGSTDN
                     SGSICDELAKTDNRIKVIHKENGGLSDARNIGIEVAKGDFIGFIDSDDYIDEDMYAFL
                     YNNILKYDAELSMCGIYDVHKNKEIKKLTPFSQLVTKSEAIELVLDGKLVVANAVSKL
                     YKKELFENVRYPKGKIAEDAAVILKIINQCNKIHVDTSQKYYYYHRGDSITSKKFTKK
                     DFDIIDVWNENEKWIIKNYPQMYDKVHTRVCWAYFVVLDKLVLTEEKGLYQEKKQVRE
                     YLIDNFFFIIKNKRLTFQRKISMLLLTFGIAAYKIPVKYQYKKVKAVN"
BASE COUNT          765 a          180 c          344 g          670 t
ORIGIN      
        1 tttgttgaca gtgatgatta tattgatgaa gatatgtatg aaattttgta tgagaatttg
       61 aaaattcatg atgccgacat tagctctgtt gaattgattc ctttttatgg tgatagatat
      121 aagaaagcta ataaagaaaa gaaagtaatt atattaaata aaaaggaagc aataaaatct
      181 gttttagaag gaacgcaatt ttatgcatac gcatggaata aactttatcg taaggaactt
      241 tttaaagata atcgttactt ggatggaaaa acatttgagg atgcatatat aataatagat
      301 ctattatttc aaacagaaaa aattgttgtt tccaatgaag agaaatattt ctatcttcaa
      361 agaaatgata gtattatggg aaaaagtttt tctatgaata atttcgatgt tatagaagca
      421 tggcaatata acaaagagaa gattttagat gcttttccag atttgcatga ttcctattat
      481 cgccgactat gttgggcata tttttacgtg ttggataaaa tggttttatc aagtagttat
      541 caacaaattc cacgtaaaaa aacagtaatt gaatttttaa agaagaacag aaattttata
      601 ttaacttata agggattcac taaatcgcgc aaaatagcta ttattgctct gagctttaga
      661 gtagattggt ataggaaatt ggttgaacaa aaaaataaaa aaaattagaa tgtttaaatt
      721 agtaaataaa agaaaaggaa ttgaaaacta aaatgtgcag aataagtata atagttccag
      781 tttataatgt tgaaaaatat ttaaaaaagt gtgtcgattc aattctaggc caaacattta
      841 ccgattttga attattatta attgatgatg gttctacaga taattcagga agtatatgcg
      901 atgaacttgc gaaaacagac aaccgaataa aagtgataca taaggaaaat gggggtctaa
      961 gtgatgccag aaatattggt attgaggtag caaagggaga ttttattggg tttatagata
     1021 gtgatgatta cattgatgaa gatatgtatg catttttata taacaatata ttgaaatatg
     1081 atgcagagtt atcaatgtgt ggtatttatg acgtccataa gaataaagaa attaaaaagt
     1141 taactccttt ttctcaattg gttactaaat ctgaagcaat tgaattagta ttagatggta
     1201 agctagttgt tgctaatgca gtaagcaagc tatataaaaa agaattattt gagaatgtga
     1261 gatatccaaa aggaaaaatt gctgaagacg cagcagtaat actgaaaatt atcaatcagt
     1321 gtaataaaat ccatgttgat acttctcaaa aatattatta ttatcatcgt ggagatagta
     1381 ttactagtaa aaaatttaca aaaaaagatt ttgatataat tgatgtatgg aatgagaatg
     1441 aaaaatggat tattaaaaat tatcctcaaa tgtacgataa agtacatact agagtttgtt
     1501 gggcttactt tgttgtgtta gataaattag ttttaactga agaaaaagga ctgtatcaag
     1561 aaaaaaaaca agtaagagag tatttaattg ataatttctt ttttattata aaaaataaac
     1621 gattaacttt tcaaagaaaa atatctatgt tattgcttac ttttggaatt gctgcgtata
     1681 aaataccggt taaatatcag tataagaaag taaaggctgt taattaatgc tttagttagg
     1741 atgaattaga tgaaaaaaag caggttagag tattctttaa taaattcttc tatatcaatg
     1801 tttatatata ttttaaggat agtaatacaa tttgtaggta gaagtttttt tatttatttc
     1861 ttaggagcga aatatttagg attaaatggt ttatttacaa atatacttag tttcttgtcg
     1921 ttggctgaac taggcatagg ctcttctatt atttattcc
//
LOCUS       DACDRN010000158         1922 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDRN010000158 DACDRN010000000
VERSION     DACDRN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.166"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            271..837
                     /gene="amaP"
                     /locus_tag="IWJ80_002827"
     CDS             271..837
                     /gene="amaP"
                     /locus_tag="IWJ80_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9462480.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            850..1038
                     /locus_tag="IWJ80_002828"
     CDS             850..1038
                     /locus_tag="IWJ80_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9462481.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1051..1608
                     /locus_tag="IWJ80_002829"
     CDS             1051..1608
                     /locus_tag="IWJ80_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9462482.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1640..1882
                     /locus_tag="IWJ80_002830"
     CDS             1640..1882
                     /locus_tag="IWJ80_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9462483.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          707 a          296 c          395 g          524 t
ORIGIN      
        1 taaatagaat cttgtaataa taactgagca taatctttgc aatttttaat cttgaataat
       61 atcctttaag tatagccagc aatcaaaagt aaatagagaa taagtaagca attgttattt
      121 cagtatcaga ctggcaataa aacgccgtta tgatccagca atgtttggta gcgctttatt
      181 tactgatgct atgctaactg ttcgctatct atactgtgat tgtcggtaga gttatttaca
      241 gggtaaaaat aagtttatag gaggtggaaa atgaataagg ggttaaaaat aatcggtgtt
      301 atcatgagtt tactgctact ttcagtattt ctagcgattg cgttgatcaa tgcgccgtat
      361 attatgcctg aacaattgga acgatttcgc ttctttactt taacgaatta ctacatgcaa
      421 caatatattt tctgggcagc tgtagtattt gccgttcttg ctattatcat cttgctagtc
      481 gttcttttct atccaaagtc aagaggaact ttcgttatga aacgggaaga tggcaaacta
      541 acaatcgaca aaaaagcgat cgaagggttg gtacgttccc atttacacga agaagagttc
      601 attcattcac ctaaagtacg gatacgttca acgaaaaatc gaatccatat ccatgtcaac
      661 ggagacttga aacgaacttc ttcattagta gggaaaacag gtgtattgat gcaagatatc
      721 gaagaagaag taactaaagt actcggaaca aaagaaacgg tcaaagtggc agtcacatac
      781 agcggttatg aagaacaaga agatacaaga gactacaagc actctcgagt ggaataggag
      841 ggccaaataa tgaaagaact attttcagca tacaagctcc ctattatctt tgggggtctt
      901 ggactactgc tagctgtttt atggttaacg attggatttt tcaagacatt gttgatcata
      961 atcatgacag caattggcgc aggaatcggt ttttatttga aacagtcagg aatagttgat
     1021 caatttttta aatcatagga ggaaacaaag atggaaaaca aaaacatgaa tacaacaaat
     1081 ggatcagttt tggcaaatga aaaaggagaa aaccgcatga tgaatggtga attgacttat
     1141 gaagacaaag tcatccaaaa aatcatcggt tatgcattga aagatgtaga tggtctattg
     1201 acattagatg gcggattttt ctcaaatatg gctgaaaaat tagtcaacac agataacgta
     1261 acagcaggta tcaatacaga agtaggtaag aaacaagtag ctgtggatct agatgtgatc
     1321 gtagaatacg gtaaagacat cgaagatatt tacaaccaaa tcaaagaatt gatcagtact
     1381 gaagtaaaca atatgacaca tttagatgtt atcgaagtga atgtgaatgt tgcagacatc
     1441 aaaacacaag aagaatacca aaaagatcaa gaaacagtac aagacaaagt aacagaagca
     1501 gcaaaatcca caggacaatt tgcttcacgc caaacagata aagcaaaatc tgctgtaggc
     1561 aaaggtgcac aaaaagtcaa agaaaataac gaaccacgtg tacaataaga agtaagaagt
     1621 cagttaggag gaatttaaaa tgggatttat ttgggcatta atcgtcggcg gtatcattgg
     1681 agcaattgca ggagcaatca cgaataaagg atcatcaatg gggatcatcg caaacatcat
     1741 tgctggttta gtaggttcag caattggaca agctttacta ggaacttggg gaccaacaat
     1801 tgcaggaatg gctatcattc catcattgat cggtgcaatc attttagtat tagtcgtttc
     1861 attctttgca cgtaaattat aagcaaattg aagaaaaact tatgttgaaa tgaagaacaa
     1921 at
//
LOCUS       DACDRN010000159         1904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDRN010000159 DACDRN010000000
VERSION     DACDRN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.167"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            51..260
                     /locus_tag="IWJ80_002831"
     CDS             51..260
                     /locus_tag="IWJ80_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462484.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="IWJ80_002832"
                     /pseudo
     CDS             467..667
                     /locus_tag="IWJ80_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1903)
                     /locus_tag="IWJ80_002833"
     CDS             complement(1379..1903)
                     /locus_tag="IWJ80_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9462485.1"
                     /translation="MDKLFQEKASGKDIQRPEFQKLLNYIREGDCVIVASLDRLGRDY
                     EDIKNTVTFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSLLSYIAQNEREKIR
                     ERQRQGVLLAKKAGRYKGRPIEYSENSTDPQKRLVFKTVVDMLNQGVPVAEIAKENGL
                     SRPTIYKIKKANDL"
BASE COUNT          619 a          324 c          346 g          615 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatg
//
LOCUS       DACDRN010000160         1863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDRN010000160 DACDRN010000000
VERSION     DACDRN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.168"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            351..1151
                     /gene="aph(2'')-I"
                     /locus_tag="IWJ80_002834"
                     /pseudo
     CDS             351..1151
                     /gene="aph(2'')-I"
                     /locus_tag="IWJ80_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            1135..1527
                     /locus_tag="IWJ80_002835"
     CDS             1135..1527
                     /locus_tag="IWJ80_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9462486.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            1528..>1863
                     /locus_tag="IWJ80_002836"
     CDS             1528..>1863
                     /locus_tag="IWJ80_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9462487.1"
                     /translation="MNIVENEICIRTLIDDDFPLMSKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGI"
BASE COUNT          755 a          156 c          320 g          632 t
ORIGIN      
        1 aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt attgggaatc
       61 ccagcttaaa tcatagatac cgtaagggat tttattcttt atttaaaact ttgcaacaga
      121 accgtacaag atatattaaa ttgatttttg aatttttgaa aaaagaaaga aatgctaatg
      181 cagttatttt agaccctcat aaaaataatc caagagcaat aagggcatac caaaaatctg
      241 gttttagaat tattgaagat ttgccagaac atgaattaca cgagggcaaa aaagaagatt
      301 gttatttaat ggaatataga tatgatgata atgccacaaa tgttaaggca atgaaatatt
      361 taattgagca ttactttgat aatttcaaag tagatagtat tgaaataatc ggtagtggtt
      421 atgatagtgt ggcatattta gttaataatg aatacatttt taaaacaaaa tttagtacta
      481 ataagaaaaa aggttatgca aaagaaaaag caatatataa ttttttaaat acaaatttag
      541 aaactaatgt aaaaattcct aatattgaat attcgtatat tagtgatgaa ttatctatac
      601 taggttataa agaaattaaa ggaacttttt taacaccaga aatttattct actatgtcag
      661 aagaagaaca aaatttgtta aaacgagata ttgccagttt tttaagacaa atgcacggtt
      721 tagattatac agatattagt gaatgtacta ttgataataa acaaaatgta ttagaagagt
      781 atatattgtt gcgtgaaact atttataatg atttaactga tatagaaaaa gattatatag
      841 aaagttttat ggaaagacta aatgcaacaa cagtttttga gggtaaaaag tgtttatgcc
      901 ataatgattt tagttgtaat catctattgt tagatggcaa taatagatta actggaataa
      961 ttgattttgg agattctgga attatagatg aatattgtga ttttatatac ttacttgaag
     1021 atagtgaaga agaaatagga acaaattttg gagaagatat attaagaatg tatggaaata
     1081 tagatattga gaaagcaaaa gaatatcaag atatagttga agaatattat cctattgaaa
     1141 ctattgttta ttgatgataa tatggttttt gatattgata atttaaaagg atttcttaat
     1201 gataccagtt catttgggtt tatagctaaa gaaaataata aaattatagg atttgcatat
     1261 tgctatacac ttttaagacc tgatggaaaa acaatgtttt atttacactc aataggaatg
     1321 ttacctaact atcaagacaa aggttatggt tcaaaattat tatcttttat taaggaatat
     1381 tctaaagaga ttggttgttc tgaaatgttt ttaataactg ataaaggtaa tcctagagct
     1441 tgccatgtat atgaaaaatt aggtggtaaa aatgattata aagatgaaat agtatatgta
     1501 tatgattatg aaaaaggtga taaataaatg aatatagttg aaaatgaaat atgtataaga
     1561 actttaatag atgatgattt tcctttgatg tcaaaatggt taactgatga aagagtatta
     1621 gaattttatg atggtagaga taaaaaatat acattagaat cattaaaaaa acattataca
     1681 gagccttggg aagatgaagt ttttagagta attattgaat ataacaatgt tcctattgga
     1741 tatggacaaa tatataaaat gtatgatgag ttatatactg attatcatta tccaaaaact
     1801 gatgagatag tctatggtat ggatcaattt ataggagagc caaattattg gagtaaagga
     1861 att
//
LOCUS       DACDRN010000161         1848 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDRN010000161 DACDRN010000000
VERSION     DACDRN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.169"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..827)
                     /locus_tag="IWJ80_002837"
     CDS             complement(<1..827)
                     /locus_tag="IWJ80_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9462488.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDI"
     gene            complement(829..1497)
                     /locus_tag="IWJ80_002838"
     CDS             complement(829..1497)
                     /locus_tag="IWJ80_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9462489.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1628..>1848)
                     /locus_tag="IWJ80_002839"
     CDS             complement(1628..>1848)
                     /locus_tag="IWJ80_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9462490.1"
                     /translation="TKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYATFYDILK
                     HSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          664 a          304 c          238 g          642 t
ORIGIN      
        1 tttatgtctt ttagaatatt ttcataagta atttcttcaa cttttaatac atctgcactt
       61 accttttcga tatagggaac ttcaatcgaa ctattaaaaa tgtcaaattt ccttaatgcg
      121 ttattgtttt cagtttttaa caactgaata gttgtagttt ttacagttct atttttaaca
      181 aatagaactt gatcaattaa ttctttcaaa tagtaaaaac gatgttcagc tataacaata
      241 gtaaatcctt tattttttaa atcacaaagt aagtcagcaa gtttcttagt cgctttgaag
      301 tccaaatttg aagacggttc atccaatata attaatttag agtttagcga taaactacat
      361 ccaatagaaa ttttctgccg ttcaccactt gatagcgaaa aaatgttttt atctaatata
      421 tttgaaagat ctaataattc agctaattca ttgattcttt tatccatatg ctcttttgta
      481 aatccaaagt tttccatcga aaatactaac tcactcgttg tatttgttgt aaagaattgt
      541 cctctaggat cttgacttac gtagccaata ttttttgata tactttcttg tgttaaggat
      601 tgtagcgagt tattttcaaa taaaatttcc ccaaccaatt ttccgtcata aagtcttgga
      661 ataagtccat tcattaactt taatagtgtg gatttcccac tcccagaaga accagtaatc
      721 aatgtaagac tacctttttt tattgaaaaa ttaaggtcaa caagcgcttg ttccttaaca
      781 tccttatatg aaaaagaaac attgttaaat tcaaacaatt ctttcacctc aaatcaaaat
      841 acttaagaaa aaaatagtaa cggaaaacag gatgatatag tctaataagc tcatgtttgt
      901 tttatagtaa gaacttcttt tcacaggaga ttctatcccc tttgttattg cattagcggt
      961 catttcttca gcaatcatag ttgcacgtat aagaacggga actaacagaa attccaaata
     1021 ttgcagaggc tgaaaaaaag acaagcgaat gtttctcatt tttaaagatt ctttaatcat
     1081 tcttatttca gaacgataag tcggaaagaa tctgatcact actgtaatac ttaacattat
     1141 ttttttcggt aaatgtattt tttctaaaga ggataataat tcattgctag tatagacact
     1201 aaccaacaac catgagatca ttacgaccgg caacattcga aatacaatat ataaggatgt
     1261 cccaaaaaat atagtaaaaa actctatctt gatagcatca ataattttca ttactaaaaa
     1321 cagaactgaa taaatcaaga tgaaataaat acatgtcttt attttacgaa acatcaatac
     1381 taaaactgta gcaataatga aacaccccac ttgaaatata tttccctttc ctaaaaaaat
     1441 aatgaccgga aaaaaaatat ttaaagtaat caaggttaac ggattgattt ttgacactct
     1501 ttcacctcca aaaaataaga tcgcactgtt atcttaagct gataaaaatg caccgttatt
     1561 cagataacag tgcgatctta tcaaaaagca taactgagaa caattctcaa tgcaagcttt
     1621 tttttaatta actcagtaat tcaatccacc caggaacaga aaattgatac aatcttctgg
     1681 ttaatagcat agtcttttct tttgaatagc tatgcttcaa tatatcatag aatgtagcgt
     1741 agaatgagta aatgtagaag tggatttctt cttcattaaa gactaaattt tgattgatca
     1801 ttccgatagt ctttaactta tctagacaac agactgtctt ttttgtca
//
LOCUS       DACDRN010000162         1817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDRN010000162 DACDRN010000000
VERSION     DACDRN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.170"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..518)
                     /locus_tag="IWJ80_002840"
     CDS             complement(<1..518)
                     /locus_tag="IWJ80_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP9462491.1"
                     /translation="MTINEVIYSPKHPLFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLGNLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPL"
     gene            complement(569..1390)
                     /locus_tag="IWJ80_002841"
     CDS             complement(569..1390)
                     /locus_tag="IWJ80_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9462492.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLGCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(1387..>1817)
                     /locus_tag="IWJ80_002842"
     CDS             complement(1387..>1817)
                     /locus_tag="IWJ80_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9462493.1"
                     /translation="SGDQKGIINLHFVMWFVHYALYALIPFFAVLFGADVVKSVLDNI
                     PEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLELPLIAIAAVGT
                     IIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
BASE COUNT          574 a          418 c          305 g          520 t
ORIGIN      
        1 tacaaaggca catctttcac tgaaacttga aggctttcgt gtgtttcatt cacaatgtaa
       61 acctgttgaa caagcatact taaacgctta ttagccagta ttggagcaaa ggatactaca
      121 atgaccttaa acgtcgaata atgatgaata tatctaggca aacagtcttg aatcacatcg
      181 acttcaatac gatgatccga ttctgacaaa tggtagacaa tcccgtttac ttcaaccttt
      241 aaggataacg aattggtttg agaaagctgt ttattgaccc aaacacctgg tttttcctca
      301 gttagtaaat tttctcgata aaaggtcaac gtttgaatcg tcccgcaaac atcaaggttt
      361 cctaatgtct tttgattact tacatcgaag gtcaatccct ttacatcagg aatattccga
      421 tagtagctgt attcgtttga agtagtcaca tttccgtcgt ttaactcagc attaaataat
      481 gggtgtttgg gtgaataaat tacttcatta atagtcatct tttccctcct tgctaaaaaa
      541 actgcagcct agtagctgca gcttcctact atgcgattcc aagtaatgac aataaaattc
      601 ccaagacgac cattccaaaa ataattttga tgacgccgac ttttttatct agtaatttga
      661 aacagcccaa tgttaccaat aacggtacaa ttcctttaaa aatactatcg aggatttctt
      721 ggacattcat tagttcttcg cccttcattg aaaagctcaa aacggtattg aatttgacca
      781 tactgatcgt cattgcgcct accatgatca gacccaagat actagcagct tttgtcagaa
      841 tttcaattaa cccgttcgca tacagctttt caatatattt agagcctaat gaatagccta
      901 aaacgccgcc atagtatcgc atcaaataat taggtatatt gaacataact aaaaagataa
      961 ttggtgccaa aatatttccg gcattggcta agttgatcgc tagtcccgcg gtaacgacac
     1021 gccaaactcc ccagaacaat gaatcgccaa ttccagccaa tggtcccatc aaactcgttt
     1081 tgatcgcgtt gatactttcc acgtcaaaac cagctttttc agaattttcc ttctccatcg
     1141 atgccgcaat ccccatgaca aaggacgcgc aaggaatggt cgtcgaaaaa tactgtgtat
     1201 gacgttccag cgccgcagct cgatcttctt cacttttgta gaaatgatta atgaagggca
     1261 tcatagaata ggcgaaacca ccgccgccca tcgtcaccgg attcaccgaa gcatacatag
     1321 tcatcgagcg ccaaaacatc ttccgaacca attttttctc atctttagaa attccatcag
     1381 ctaatttcat tgaaagaaat cctcctcttc ttggtcaagc gttgcattta cagcagcatc
     1441 gctttgaact gcatgaacag atatattttt tagttgtaga tctctttggg ctataacaat
     1501 tgcaataatc gttccaactg cggcaattgc aatcaatggt aactcgagat acgccataac
     1561 aacggcccca aagaaaaacc acacagcaat atttttactc caaagcatcc gtaacagcat
     1621 agccatccca actgccggca gcagatttcc agcgactgtt aatccgttca tgatgacctc
     1681 tggaatatta tccaagacgc ttttgacaac atctgctcca aataaaacgg caaagaatgg
     1741 aataagagca taaagcgcat agtggacaaa ccacataacg aagtgcagat tgatgattcc
     1801 tttttgatca ccagatt
//
LOCUS       DACDRN010000163         1696 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDRN010000163 DACDRN010000000
VERSION     DACDRN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.171"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..759
                     /locus_tag="IWJ80_002843"
     CDS             <1..759
                     /locus_tag="IWJ80_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9462494.1"
                     /translation="DGTKDTVKVPVEVTDNRSDADKYNPEGQKVTTDLNKEPDASEGI
                     KNKEDLPKGTTYTWKEKVDVSTAGNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQ
                     VDPITEGDQIVTGKTEPNAEVTVTLPNGSQYHGTADKNGNFTVKVPKLEAGTKVIVTA
                     TDESGNTSEPTNVVVSSNEKDSEKAVSKDNKTDNQGSKQNKNRGKSSPQKQSSKAYPK
                     TGEIDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            893..>1696
                     /locus_tag="IWJ80_002844"
     CDS             893..>1696
                     /locus_tag="IWJ80_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9462495.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNE"
BASE COUNT          679 a          211 c          337 g          469 t
ORIGIN      
        1 gatggtacga aagatacagt gaaagttcca gtagaagtga cagacaatcg ttccgatgcc
       61 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagacgca
      121 tctgagggaa ttaagaacaa agaagatcta ccaaaaggaa ctacttatac ttggaaagag
      181 aaagtagatg ttagtacagc tggaaacaaa aaagggattg tagtagtaac atatccagat
      241 ggatctaaag aagaagtaga ggttactatt tctgtagaag ataaaaaagc accgaataaa
      301 cctcaagttg atcctattac agagggtgac caaattgtta ctggtaaaac tgaaccaaat
      361 gcagaggtga cagttacatt accaaacggg agtcaatacc atgggacagc tgataaaaat
      421 ggtaatttta cagttaaagt tcctaaatta gaggcaggaa caaaagtgat agtaactgca
      481 actgatgaat ctggtaatac tagtgaacct actaatgtag ttgtttcttc aaacgaaaaa
      541 gacagtgaaa aagccgttag taaagataat aagactgaca atcaaggtag caagcaaaac
      601 aaaaatagag gcaaaagcag tcctcaaaag cagtcctcaa aagcttatcc taagacggga
      661 gagattgata gtaatatctt tactattagt ggtggtttaa tcctgttagg aactttaggg
      721 ttattagggt ataaaaatcg aaaaaaagag aatgaataga aaatcaattt cttaaatctt
      781 ctctgaaaag aggaagaatc tttttcttcc tctttttgtt ttttattttg ggagataggt
      841 tgttagttaa aaatgaagat acttttcata gaagggaaaa gaatgtgatt aagtgaaaaa
      901 aatagtaatt attggatcaa accattcggg gctagctgca gctcgatttc taaacttatg
      961 tgattcaaat tatgaagtta ttctgataga taaaaaatca gaaattagtt acttagaatg
     1021 tgggacatcg ttgttcataa aaagagaagt tagcaattta gaagatcttt tttatcaaaa
     1081 tagaagagaa ctaaattcta agataaaaaa gttgtttttg gagacagcag ttgacaaaat
     1141 tgatattcaa aataaaaaga ttatttgtgt aaaaaaagat ggcacaagat tttcccaaga
     1201 atatgacaaa cttatactag ctacaggttc aaagcaaatt atgttagatg taccggggaa
     1261 taatttagct aatatttttt cgataaagaa ttttccaaat gctaaagaga tttttaaagc
     1321 tttagatgat aatactatta aaaaagtagg aattattgga gcaggatata ttggagttga
     1381 aattgctgaa gccataagaa aaagagggaa agaagtatat cttttcgatg ttgcagatag
     1441 agtgttgtcg acttattacg accgtagttt ttcagataaa gttgaagaaa tactatcaaa
     1501 aaatggaata catctttgtc tatcagaaag tcctaaaaaa tatttaggaa gaaaaaagat
     1561 tgaaaaaata gtcactacaa gtggtaccca atactctatg gatatggtcg tacaagctat
     1621 aggatttcta tctaatagtc caattggcaa aaaggaactc ttgagagatg ttagtggagc
     1681 atatctaact aatgaa
//
LOCUS       DACDRN010000164         1666 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDRN010000164 DACDRN010000000
VERSION     DACDRN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.172"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..345)
                     /locus_tag="IWJ80_002845"
                     /pseudo
     CDS             complement(1..345)
                     /locus_tag="IWJ80_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            620..1162
                     /locus_tag="IWJ80_002846"
     CDS             620..1162
                     /locus_tag="IWJ80_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462496.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1252..>1666
                     /locus_tag="IWJ80_002847"
     CDS             1252..>1666
                     /locus_tag="IWJ80_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP9462497.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          607 a          220 c          316 g          523 t
ORIGIN      
        1 tatttagaat tttgcacatc gctgatatgg aatatttatg cttgttggca tcaattactt
       61 gtcttttcgt ccgaatatca gcgccgcttg ctttaaaata tcattctcca tttcgagttg
      121 cttatttttc tttcttagtg cgatcaattc tgcttgttct ggtgttaagt tgtctctttc
      181 tttgaatgag cccgttgatt gtgcttgctt catccattta tcgaaagaag aaggcgtaag
      241 ctcatactct cgaataattt ctgcgcgagg cttaccagcg agatagagat cgacgatttg
      301 ttgtttgaat tcttttgaat aggttcttcg ttgacgtctt gacatgaaaa atcctccagt
      361 gtgtttttta ttattctaca caccttattt tttctgtcta gtctagtgta gccgattcaa
      421 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
      481 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      541 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      601 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      661 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      721 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      781 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      841 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      901 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      961 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
     1021 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
     1081 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
     1141 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
     1201 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
     1261 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
     1321 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
     1381 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1441 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1501 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1561 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1621 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACDRN010000165         1636 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDRN010000165 DACDRN010000000
VERSION     DACDRN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.173"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(183..1502)
                     /locus_tag="IWJ80_002848"
     CDS             complement(183..1502)
                     /locus_tag="IWJ80_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP9462498.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          485 a          301 c          325 g          525 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaat
//
LOCUS       DACDRN010000166         1540 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDRN010000166 DACDRN010000000
VERSION     DACDRN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.174"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            382..999
                     /locus_tag="IWJ80_002849"
     CDS             382..999
                     /locus_tag="IWJ80_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9462499.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1040..1339
                     /locus_tag="IWJ80_002850"
     CDS             1040..1339
                     /locus_tag="IWJ80_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462500.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1373..1540
                     /locus_tag="IWJ80_002851"
     CDS             1373..1540
                     /locus_tag="IWJ80_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9462501.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
BASE COUNT          547 a          224 c          311 g          458 t
ORIGIN      
        1 tcaaaaagat ttatttttaa ccctaaatta gaaatatata gtgttgtaat agaataatag
       61 tgttaaaaaa attgtaaaag ttaaagaagt caaattctga tgtgaaaaat aaaatatacc
      121 atggaagaca taaaagaaaa actggataac tagtttattt aatggcagac cgttaaggcc
      181 tgccattttt atgtatttta gcggaataaa tataatcatg cgggagtgta aacttactgt
      241 ttcaaaaagt atactttttg acatgccttg attttctttg tttatgtatg gcataataac
      301 cacataatac aatttgaaac gtttctgatt taatctgatt gttttgaaac aattttagtg
      361 aaaaaagggt agataaaaac gatgagaaaa tttttttata tccgggtcag tagttatgat
      421 caatcgatcg aacgacaact gatttccgcg aaggaattag gaatccctga agaatatata
      481 tttgtagaaa aagccagcgg caaagatttt aaacgccctg aatatcaatt aatgaaacgg
      541 atgtttcgaa aaggagattt actctatcta caatcattag atagactagg cagaaataaa
      601 caaatgattt tagaagaatg gcgagaatta actaaggaaa agcagattga tatttctgtg
      661 ttagacatgc ctctgttaga tactgctagt tacaagcaac tagatgggtt agagacgctt
      721 atctctgact taattctgca attactcagc tatatggccg aagacgagcg gaaacgaatt
      781 aatagccggc aaagagaagg cgtagaagct gcgaaacaaa agggtgtaaa attcggcaga
      841 aagaaaattg ctttacctcc tgaatttcct gcgatctatt ctgactggaa agcaggaaag
      901 ataactgcag tctatgctat ggatcaattg ggcatgaaac gaaatacctt ttatcgacgg
      961 gtgaaagaat atgaacatac cttgcaccac ttttcttgat agatataaca gctattagac
     1021 gggaagggag agtttgaaaa tggacttaac gaatcaaagg gtcgttttta ctggaacgct
     1081 gcagcaaatg acacgaaccc aagccatcgg tttggttcac tctttaaacg gccattgtca
     1141 atcaagtgtt acttcaaaaa caaatttcct ggtttgtggt cagtattcta agtcgttgtt
     1201 tgatgattct ctctatacaa aaaaggaaca aaccgcgcgg gacttgattg cgttaggtca
     1261 tgagattacc attttatcag aagtcgaatt ttatgcgtta attagtcaac agttgaaaga
     1321 atggcaacgg tatttataat cgttagtaaa atttacaagg aggaaaattt ttatgaagcg
     1381 aaaatcttat gatcgttggg gcattgaaat aactagtttt aatcgaaata ttgcttttca
     1441 cgtggagtgt gactgtgggg aattagcaaa aagtgtttat caaaaaaatt acttttactg
     1501 tcctaaatgc catcgccgat acggggtaca tggaacatag
//
LOCUS       DACDRN010000167         1517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDRN010000167 DACDRN010000000
VERSION     DACDRN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.175"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            288..695
                     /locus_tag="IWJ80_002852"
     CDS             288..695
                     /locus_tag="IWJ80_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462502.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="IWJ80_002853"
     CDS             709..1110
                     /locus_tag="IWJ80_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462503.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="IWJ80_002854"
     CDS             1112..1483
                     /locus_tag="IWJ80_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462504.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          576 a          185 c          264 g          492 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat tttttta
//
LOCUS       DACDRN010000168         1509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDRN010000168 DACDRN010000000
VERSION     DACDRN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.176"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            246..941
                     /locus_tag="IWJ80_002855"
     CDS             246..941
                     /locus_tag="IWJ80_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462505.1"
                     /translation="MRSSKYTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVL
                     KGIEIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFA
                     QLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKS
                     AYLYDNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKAKGVTELAI
                     YQKRKRIMVPLPV"
     gene            complement(978..1475)
                     /locus_tag="IWJ80_002856"
     CDS             complement(978..1475)
                     /locus_tag="IWJ80_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9462506.1"
                     /translation="MNNQIALAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT          482 a          265 c          240 g          522 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttacact ctctaataat
       61 tacacacaca caatttttta aggtcttaaa accatcatat atgcaaaaga agctaacctt
      121 tattactaaa ttagacccgt ggtgctagtt aattttttcc tagaggaaaa tgtctaaaca
      181 tgttggtaaa tcaacaaaaa aacgcatata gtagtctcaa aactcccaag aaatgaggac
      241 tacatatgcg ttcatcaaaa tatactgaac attttgactt agccaatcct gttactaaag
      301 tcgatgatat ccccgattat gaaatgtaca gtcaaacaat cgatagctta aataagcgtt
      361 ttggcaatcg tgttttaaaa ggcatagaaa tcggttacat cgcttctgaa aaggatcgga
      421 tcatcgacta tttagccgat aaagattacg atttaaaatt actcagtgtg catcataatg
      481 gtcaatttga ttatttagat gacgaagtca aagatatgga tcctgctatt gtgattcctc
      541 agtattttgc acaactttca gaagcactag ttgttataga agcagatgtc tttgctcatt
      601 ttgactatgg ttttcgggta tttggtctat ccgttgctga atttaaacaa tatgaagcgc
      661 agtttttacc gattctagat caagtaatca aaaataaatt ggcattcgaa ttaaatgcta
      721 agagcgctta cttgtatgac aatttagcgt tatatgaata tgtcatcgat ttatatcttt
      781 cacgtggtgg taccttgttt agtgttggat cagatgggca ttaccttgaa catttccgtt
      841 tccatttcga tgatcttttt gctctgttaa aagcaaaagg tgtgacggaa ttagcgattt
      901 atcaaaaaag aaagcgaatt atggttccat taccagtcta aaaaaacgtc ctcagcatca
      961 aaagtgagga cgttttttta gatttctttt tctaaaatcg gacaaatacg atcttgtttt
     1021 cctaaaggaa aatgtttgat cccgattttt ttaaatccat gacgttcata aaaacgaata
     1081 cctgcatgat ttttttcata aacagtcaaa cgtaaataac gtttttgttt cgttttagca
     1141 taacgattcg cttcatcgag taaccgttgc cctaaccctt tgttttgata atctttgagc
     1201 agatagagcc gttgaatctc tatagcatct ttttcatcat tgactttcat ataaccaatg
     1261 agtatttgat gatccttaat accaataaat ttactggttg actgtgccaa ttctactgcc
     1321 aatatttcag ttgtcaattg tgtctgtaaa tatccttgca tatcaagtgg atctgcgcca
     1381 tcgataaacg attcataaaa actgacaaca cttacaactt gtaaagcatc tagatcatca
     1441 taatctaaat acgctagtgc gatttgattg ttcatctaac tcctcctttt gacatagtat
     1501 actataaat
//
LOCUS       DACDRN010000169         1484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDRN010000169 DACDRN010000000
VERSION     DACDRN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.178"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            196..921
                     /locus_tag="IWJ80_002857"
     CDS             196..921
                     /locus_tag="IWJ80_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462507.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="IWJ80_002858"
     CDS             911..1420
                     /locus_tag="IWJ80_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462508.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
BASE COUNT          409 a          288 c          241 g          546 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gctt
//
LOCUS       DACDRN010000170         1415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDRN010000170 DACDRN010000000
VERSION     DACDRN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.179"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            90..1382
                     /locus_tag="IWJ80_002859"
     CDS             90..1382
                     /locus_tag="IWJ80_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9462509.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          460 a          288 c          256 g          411 t
ORIGIN      
        1 atctttttta ttttccaaaa catatagaaa agacaccttg aaatccttta gaattaattt
       61 ggcgaaccaa cccaaaagga ggaatcaaga tgtcttacac tcaccttatc aaagaaacat
      121 tagatattct agacttatca gttactttta acgaaaattg tctcacaaaa gaaaaataca
      181 aagggcaaat ctgtcatatt tatcgtggaa acttgattta tacagcccaa gaatgcatcc
      241 actgtaaaca ccagattgct tcagatattg tgcgttgggg tacaacaacc gtccggttgt
      301 taatgaacga tgtttctgaa taccgtacct acctcgaatt gaaaaagcaa cgcttcaaat
      361 gcaaagcttg tcagcggacc tttgtcgcag atacttctgt tgcgaaaaaa cattgtttca
      421 tcagtgagaa agtccgctgg tctgtcgtga ctcgtctgaa aaaaaatacc tcaatgacag
      481 agatcgccgc tcagaaaaat ctatcggtct cttctgttta ccgtattatg aagcgatttt
      541 atcgtccgtt gaatcctttt cggacaccgt tacctaaggt actttgtttt gatgaattca
      601 aatcagttcg gggtgtttca ggtgctatga gtttcatcat gatggatgga caaacacagc
      661 gcttattgga cattgtcgag aaccgacaac tgcctttttt aaaacgttat tttagtcatt
      721 tttcacgaga aattcgtgaa gcggtcgaat ggatcgtgat tgatatgtac gctccttatg
      781 tatcgctcgt taaaaagctg tttccaaaag cacagctgat tattgaccgg tttcacatcg
      841 tccaacatat tggtcgtacc tttcgcaatc atcgaatcaa agaaacgaac caactattaa
      901 aaagcaaaga acaaaaacac tatcaattag gtaagcaatt aaaaagatac tggaaacttc
      961 ttcaaaaaga tgaacgcaag cttgattata ctcgacgcct ttggcgacct ggcttcaaag
     1021 ctcatttaac agagaccgat attgtggatc gcttactgaa agggagccct gctttgcgtg
     1081 taggctatca gttatatcaa gactttcttt acgctgtaaa ggaacgagac tacgtatcgt
     1141 ttgaagaact acttaccaat aatatcatgc ttcccgaagg ctatcagacg accttgagaa
     1201 cgttccagaa atttctgcca caaatcaaaa atgctttaca acaaagttat tcaaatggcc
     1261 cacttgaatg tctgaacaac catatcaaag tgttaaaacg aaatgcatat ggctttcgta
     1321 gcttttataa tttcaaattg cgcatcatga ttcgtcatgg caacgcattg attttcaact
     1381 aaaaaagatc aagaggaact gatccacttg atctc
//
LOCUS       DACDRN010000171         1409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDRN010000171 DACDRN010000000
VERSION     DACDRN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.180"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1027
                     /locus_tag="IWJ80_002860"
     CDS             <1..1027
                     /locus_tag="IWJ80_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP9462510.1"
                     /translation="PQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDAFALGKYHTGS
                     NLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPFGEQFLEGIGD
                     EEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMPFYQFLDNKDH
                     LYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNPQAFVTRLEEL
                     ICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQYFGLKFKKGVL
                     QIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKKILHVEKGKTI
                     HIQRRKR"
     gene            1024..>1409
                     /locus_tag="IWJ80_002861"
     CDS             1024..>1409
                     /locus_tag="IWJ80_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9462511.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPT"
BASE COUNT          425 a          243 c          328 g          413 t
ORIGIN      
        1 tccgcaggtt ctactgaatg cttcaagaat tattgaggac atattagctc aacgtcggtc
       61 aggggaacca atgctttttc catctgtttg gttatcagac gcctttgcac ttggaaagta
      121 tcataccggc agcaatcttt gtctttggaa ggcctgtatt ggacttgcag aaatttttga
      181 agccctagaa caactgagtc ttagcaagcg atacaaaaac attgcctgta aactaaagga
      241 atcaatttgt aaacaaatga ctatcgatgg tccttttggt gagcaatttt tggagggaat
      301 cggtgatgag gagaaaacaa cctattcagt gaagcactat caaaaaccga ttcttgagca
      361 agggttaatc tttttaagtg atgtgattgt ggatggaaaa attaatttat tgatgcatga
      421 tggagaagaa tcagacacta cgttgatgcc tttttatcaa tttttagata ataaagatca
      481 tttatatcag aacaccatgg ccttttctgc tagttctttc aatccaacct atagcgagga
      541 aatcaaaggc atcacttggg gattagaatc cggcgccact tttcctggct ttatcactgt
      601 tttaatgagc gagctgcaca accctcaagc tttcgtaacc cgattagaag aactaatttg
      661 tttggcagat ttagacggtt cgtggtggtg gtggccgtat cgcttagagg ctcaacaagg
      721 agaagtcgtg cgagactttg gctgcggcaa atgcggctgg gcatcgggtc tttttgtcag
      781 cctaatgatc agtcaatatt tcggattgaa attcaaaaaa ggagtattgc aaattgatcc
      841 tgttgataat ttaacgtttt cttggaagaa tttgaagttc ggtcaagcct tcatttcgat
      901 tgaatgtacg caatccgagt tgagtatttc aaatttgtca gaaaagccat tgaaaataag
      961 tgatacgaaa aaaattcttc acgtagaaaa gggaaaaacg attcacattc aaaggagaaa
     1021 aagatgagta tactaaaaaa tattacccaa tttaaaaaaa tccagagtcg agcaatcact
     1081 cccgaaaatc caacaggcga gaaaggtaaa gctgcgatgt tgccaagcga gctaggacca
     1141 agccgcaaag gtcgtggcgc aattccgctg acttctggtg aagaaactgt gattgcggat
     1201 attactggaa cgggaataat tcgtcatatg tggatgacga ttcgtgatcg aacagaattt
     1261 ggcagttttg ttttacgtga tttaatctta agaatttatt gggatgggtc ggatagccct
     1321 gctgttgaaa gtcctttagg ggatttcttt tgtaatgggt ttggagagcg ttgcgaggtg
     1381 aactcgttgc cgatagtggt taatcctac
//
LOCUS       DACDRN010000172         1408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDRN010000172 DACDRN010000000
VERSION     DACDRN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.181"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            87..1382
                     /locus_tag="IWJ80_002862"
     CDS             87..1382
                     /locus_tag="IWJ80_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9462512.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          447 a          276 c          265 g          420 t
ORIGIN      
        1 catctttttt gtataaaaaa atatagaaaa gacaccctga aatcctttag aattaatttg
       61 gctaccaatt cataggagga ttcaagatgt ctcatactca gcttatcaaa gataccctaa
      121 atattctaga cctaaatatc tatttcgagg aaaattgttt aactaaagaa aaatacaaag
      181 ggcaaatttg cttgatttac aaaggaactc ttctgtacaa gccagaagag tgttcccatt
      241 gtctttgtgt cgtcccctct aggatcattc gttgggggac gacaaccgtt cggttgttac
      301 tgaatgatgt gtctgagtat cgcacttatc tggagctgaa gaaacaacga tttaaatgta
      361 agtcttgcca aagaacattc gtagcggata cctctgttgc tgaaaaacac tgtttcatta
      421 gtcaaaaagt tcgttggtct gtcattgctc gcttaaaaga gaatacttcc atgacggaaa
      481 tcgctcgtca gaaaaatatt tccacttctt cggtctatcg tgtcatgaag cgattctacc
      541 gcccattgaa tcctttcaaa cagactttgc caaaggtctt atgctttgat gaattcaaat
      601 cggttcgaag tgttgccagt gcaatgagtt ttatcatgat ggacggacaa agccatgcat
      661 tattggatat tgtcgaaaat cgacgcttgc cttatttaga acgctatttt tctcggtttt
      721 cattagcgac tcgtgaagcc gttcaattga ttgttattga tatgtatgcg ccttatgttt
      781 cgttggtgaa aaagcttttc ccgaatgctc agttgatcat cgatcgtttc cacatcgtcc
      841 aacatattgg cagaactttc ttgaatcatc gagtgaaaga aaccacggta cgacttaaag
      901 gaaattccca ctctcaacga gggttaggga aaaaattgaa gcgctattgg aaactcctac
      961 aaaaagaaga agcaaagtta aactatgaaa aacgggtttg gcgtgccagt tttaaagatt
     1021 atttgactga aagtgagatt gccgatcgac tgttagctgc ttgtcccaac ttacgccaag
     1081 gctatcagtt ataccaagac attctttatg cggtaaagaa aagagatcag tcactatttg
     1141 aggattgttt aactagagac attagcggtc tgccagaaac ctatacaacc actttacgaa
     1201 cgtttaaaaa gtttctgcca cagatccaaa atgcgcttca ctacagctat tcaaacggac
     1261 cattggaatg cttaaacaac cacatcaaag tattaaaacg caatgcgtac ggctttcgca
     1321 acttttacaa tttcaaacta cgaattatga ttcgtcatgg caaaacattt ttaaccaaat
     1381 aaacagaaca gaaagaggtt ttcttctt
//
LOCUS       DACDRN010000173         1363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDRN010000173 DACDRN010000000
VERSION     DACDRN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.182"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(156..1297)
                     /locus_tag="IWJ80_002863"
     CDS             complement(join(156..1069,1069..1297))
                     /locus_tag="IWJ80_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9462513.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
BASE COUNT          336 a          270 c          286 g          471 t
ORIGIN      
        1 gacacgattt gtgagaattt tttcgagatt ccagcgggtg agtatctcat cgtttcgacc
       61 agcggtcaaa ccaaaaccgt ttgaccgctg atcgaagcga tgaagttgaa accctgtgga
      121 aatctcgcca aaaaaatgta agtggtagct aggaactaag cttgcaatgc caactcctcc
      181 atctgatttg gtgttaaata gttgatacta ctatggattc gcttgcgatt gtaaaatcct
      241 tcaatgtatt gaaataacgc taaataagca gcttcaaagt tttcatacgc ttttgatgga
      301 tagacttctt cttttttgag tgaggcgtga aacgattcga taccggcatt atcgtaggga
      361 cagcccttac gactgaagga gtgccgaatg ttcaattcat tcagctttgc ctcataggct
      421 tcgctagtgt actgagaacc caaatctgag tggatgatca agttctcgga gggtgtttgg
      481 tttaagagcg cattgtcaag ggtttcgata accaattctt tcgtcatctg cttgccaaac
      541 ttccaaccga tgactttctt tgtgtggagg tcttgaatag tggagaggta ggtccagcca
      601 tcttttaggg tatgaatata ggtaatatct gtcacccatt tttgattgat cgacgtagtg
      661 gtaaaatctt ggtttaacag gtttttacgt ccttcacttt taactttcgc ttgtttgcca
      721 ggcttgtact tcttgagggt gactgaacgc aagcctaatt ttctcatcag tatttgtact
      781 ttctttaagg acaaggtctc gccacggcca acaagaatct tatgaatttt aggcgcacca
      841 tagataccct tgttttcaaa gtagattcct gaaatcaagc ggtttaagcg tttgttttct
      901 gattggcgct tggaaggttt ctttttcttc cagtcatagt agctggattt cggtgcttcc
      961 aaaacggtac acatttgttt tacgtcatga tcatgaacat ttgattgaat aaattcgaag
     1021 acatcactca tttttacttt tgagcgaata tggttaaagc cttttttagg atagtgtttt
     1081 cctcctgcat gcgggccatt tcctttttca tcgctaggat ttctgcctca gtcatgccgg
     1141 ttgctacatt ctttgtatga agcttcttcc aacgataaat cgtttgtgta gcgataccat
     1201 attgcgctgc cagttcttcg accgattggc cagactggtt taaatctaag atcatcttct
     1261 taaagtcttc atcaaatttt ttcttggctt ttgccatcgt ggacacgtcc ttttcttagt
     1321 agttagattc tacggttctg ttgcaaagtt ttaaatctac tat
//
LOCUS       DACDRN010000174         1348 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDRN010000174 DACDRN010000000
VERSION     DACDRN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.183"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(124..1272)
                     /gene="tnpB"
                     /locus_tag="IWJ80_002864"
     CDS             complement(124..1272)
                     /gene="tnpB"
                     /locus_tag="IWJ80_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP9462514.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          388 a          238 c          214 g          508 t
ORIGIN      
        1 taacgcttga agtgggagct tcttgggaat agagcatact tgatagcgta tttctttact
       61 aacagcggtg tctcttattt accaagctat ccccgtagtt cctacggttc ttgatgattg
      121 gatctaagcc aatcccatag aatgtagtct tagaccttcg gtcagaatat tgatactagc
      181 attgatatca cgatcatgat gagtatgaca aacaggacaa gtccattctc gaatttcaag
      241 agatttcttg ccatctttat gtccgcactc tgaacaaatc tgactagacg gaaaccattt
      301 atctactttg atgatttttc gtccatacca gtcagccttg tattgtaatt tagtcacaaa
      361 ctttgaccaa gaaacatcag aaatactttt agctaattta tgatttcgca acataccttt
      421 tgtgtttaag tcttcaatac agataatatc gtgatttttg atcatttctg tactcaactt
      481 attcagaaaa tcagtacgtt gattcattac tttttcatgc aatctagcta ctttgcgttt
      541 ttgtttttga taatttttag cttcaaatag attggtacct ttatttttag ccaataaagc
      601 acgcctagac aatttgcgtt gttcacgctt tagtttcttt tccattttgg acgtgaattt
      661 attattatca attttttgtc catcagaaag aatcgcaaag tccataatac ctaagtcaat
      721 accaattgtg gaattagttt taggtaattc gataatttct tctttacaca acaaagaaat
      781 atagtattta ccgctagaat gacgtgatat tgtagcggat ttgataatcc cttttggttg
      841 tctatgaagc ttaattctta ctaatgactt caatttagga actttgatga atttactatc
      901 aatcaaagca actgtaccat tttgattatt cgttgtataa ctctgaacag gatttttctt
      961 acttttgaaa cgtggaaatc caacggattt atcccgaaaa aaattcttgt atgctttatc
     1021 taaattaagt tgggcattgg ctaaagcaag gctatcaatt tctttcaaaa acggaaattc
     1081 tttcttgtat ttagctggtg tcggaaaagt cattttttta gaagaatcat ttttaacttc
     1141 ttcatacgcc ttttttcggt catcaagcat tagattgtag accttacgga cacaaccaaa
     1201 agatttggca aagaagattt cttgttcctc tgttggatag attctgaatt tgtatgcttt
     1261 tagttgttcc ataaagttca cctactttct atttatgatt gtttccttga ttttggatat
     1321 acttcttaat cacatcaatt ggtgcgcc
//
LOCUS       DACDRN010000175         1289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDRN010000175 DACDRN010000000
VERSION     DACDRN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.184"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(15..1154)
                     /locus_tag="IWJ80_002865"
     CDS             complement(15..1154)
                     /locus_tag="IWJ80_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP9462515.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          336 a          281 c          239 g          433 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgcccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tattttatt
//
LOCUS       DACDRN010000176         1262 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDRN010000176 DACDRN010000000
VERSION     DACDRN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.185"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            70..408
                     /locus_tag="IWJ80_002866"
     CDS             70..408
                     /locus_tag="IWJ80_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9462516.1"
                     /translation="MNGIEDENLRRLINSLVIELYKYQAESERKKIKERQAQGIEIAK
                     KKGKFKGRQLKFKKNDPRLKHAFDLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVD
                     QRKNNEKRDS"
     gene            500..1241
                     /locus_tag="IWJ80_002867"
                     /pseudo
     CDS             500..1241
                     /locus_tag="IWJ80_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320596.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          479 a          201 c          257 g          325 t
ORIGIN      
        1 aaaatgacaa attgatgaat caaattcaaa ttaagggggg caaccctgga agtcttaaat
       61 ttaccctcaa tgaatggtat tgaagatgaa aatttaaggc gtttgattaa tagccttgtc
      121 attgaattgt acaagtatca agcagaatca gaacgaaaaa aaattaagga acgtcaggca
      181 caaggaatcg aaattgctaa gaaaaaaggc aaattcaaag gtcggcaact gaaatttaaa
      241 aaaaatgatc cacgtttaaa gcatgctttc gatttgtttt tgaatggttt atccgataaa
      301 gaagttgaag aacaaactgg gatcaatcgc cgaacgttta gaaggtatcg agcaagatat
      361 aacgtgacag tagatcaaag aaaaaacaat gaaaagaggg atagttaatg agtacggtta
      421 ttttagctga aaaacaagcc aggcattagc ctacgcaagt gctttaaaac aaagcaccaa
      481 aaaagacggt tattttgaga tcaaagaccc actatttaca gatgaaacgt ttatcacctt
      541 tgattttggg catttagtgg aattagcaga accaggtcat tatgacgaaa agtggcaaaa
      601 ttggaaactt gaatctttgc cgatttttcc tgatcgatac gattttgaag ttgcaaaaga
      661 taagggaaag cagtttaaaa ttgttgcaga acttctcaaa aaggcaaata caattattgt
      721 tgcaacagat agcgacagag agggtgaaaa tatcgcctgg tcgattatcc ataaagcaaa
      781 tgccttttca aaagataaaa catttaaaag actatggatc aatagcttag aaaaagatgt
      841 gatccgaagc ggttttcaaa atttgcaacc aggaatgaat tactatccct tttatcaaga
      901 agcgcaaaca cgccaaattg ccgattggtt gatcggcatg aacgcaagcc ctttgtatac
      961 gttaaattta caacagaagg gcgtacaagg tacattttca ctaggacgtg ttcaaacgcc
     1021 caccttatac cttatttttc agcgccagga agctagtata aaagtaaaac aagggtcatt
     1081 taagggcgtt ctaagcccca cacagcgttt taaaacccaa gaggagcttt tttgcttttg
     1141 tttcttctaa acaagctaaa ataggcaatc aagaggggat aattgctgat gttcaaacca
     1201 aagagatgga taggctacaa ttaactagac agaaaaatta aggtgtgtag actagaagaa
     1261 aa
//
LOCUS       DACDRN010000177         1230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDRN010000177 DACDRN010000000
VERSION     DACDRN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.186"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..340)
                     /locus_tag="IWJ80_002868"
     CDS             complement(<1..340)
                     /locus_tag="IWJ80_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9462517.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGV"
     gene            complement(473..1198)
                     /locus_tag="IWJ80_002869"
     CDS             complement(473..1198)
                     /locus_tag="IWJ80_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9462518.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
BASE COUNT          419 a          236 c          142 g          433 t
ORIGIN      
        1 caactcctat aaggataact cctaatgcta aaaaagaact gaaagtaata ccaattgctg
       61 tatctccttt tattacacta ttgcttttta taaaagtaat aatgatagaa gctaatagac
      121 caaatacaat cgcaccaata aaaaaattaa tacctaaaat ataagaaatt gctactcctg
      181 gcaaaacagc atgagaaata gcatcaccca taagagacat tcctcttaaa ataataaaac
      241 aacctactgt tccagaaaca atccctataa ctacagatgt aattagagca ttctgtaaaa
      301 agtgaaagtc atacagacca ttaataaaat tttgaagcat attattcccc ttgtatatta
      361 gagttagggt tttaaccttc cggttttgat tggataaccc tatgaaccat cgttatttta
      421 atttggtatt aactcatttc ttttgagata atatatttat tggattgaga ggctaagccg
      481 ttcccccctc tccaataaat atagtatctc cataggcttt ttttaaattc tcttcattaa
      541 atacagattc tacacttccg tgagcaatta gatttcgatt caaaagaata atattatcaa
      601 aatacttctt taccttgctt aaatcatgat gcacaattag cactgttttt ccctgatgtt
      661 tcaattcttt taacgtatcc ataattattc gttcactaac taagtctatc cctacaaatg
      721 gttcatctaa aaatatgaaa tccgcatttt gagcaagaca tcttgctaaa agtacacgtt
      781 gaaattgtcc tccagataat tctcctattt gatgattaga atattttccc atatcgacct
      841 tttccaaagc ctttgaaact ctttgccatt ctgcattttt aattctttga aaaactttca
      901 ttccggcata tgttcccata gatacacatt cttttacttt tattggaaaa gtaaaatcaa
      961 tatcagcttt ttgctctaca tatgcaatct tcaccagttt tttattgata tcttcattat
     1021 taatggagac ctctccctga tgagggataa tatttaacat tgctttaagc aatgtagatt
     1081 ttccagcacc atttggacca ataatacctg ttaaagcacc ttcttgtatt tttaaagaaa
     1141 tctcgttcaa tgctaaaaaa tcgttataac aaacattaag ctttttcact tctaacataa
     1201 ctttctcctt ctaaatgata atgattatca
//
LOCUS       DACDRN010000178         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDRN010000178 DACDRN010000000
VERSION     DACDRN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.187"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IWJ80_002870"
     CDS             <1..1076
                     /locus_tag="IWJ80_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9462519.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACDRN010000179         1111 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDRN010000179 DACDRN010000000
VERSION     DACDRN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.189"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(62..280)
                     /locus_tag="IWJ80_002871"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="IWJ80_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="IWJ80_002872"
     CDS             complement(546..740)
                     /locus_tag="IWJ80_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462520.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          389 a          187 c          159 g          376 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta t
//
LOCUS       DACDRN010000180         1107 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDRN010000180 DACDRN010000000
VERSION     DACDRN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.190"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            144..407
                     /locus_tag="IWJ80_002873"
     CDS             144..407
                     /locus_tag="IWJ80_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9462521.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            407..679
                     /locus_tag="IWJ80_002874"
     CDS             407..679
                     /locus_tag="IWJ80_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9462522.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            756..1085
                     /locus_tag="IWJ80_002875"
     CDS             756..1085
                     /locus_tag="IWJ80_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9462523.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          420 a          140 c          219 g          328 t
ORIGIN      
        1 aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg ctgttttggt
       61 actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt agaatggaga
      121 tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc tcagcacgga
      181 aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt gaaatcatta
      241 gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa gcaatcaaag
      301 aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga gaaaaagatg
      361 atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga ataactattc
      421 tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt ctaatttgaa
      481 acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc ctacccaatc
      541 atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga atagacaaca
      601 ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt cagcaaggac
      661 gcattacgag tcacagtaat tacttgtaaa tacattatct cgtggtataa tgtatttact
      721 aagtaattac atactgttag aaatgaggga tttaaatgat tacaaaaaca agaaaacaag
      781 gcaattcaat catgttgaca gtacctaaag attttaatgt tcctaatgga gtcgaagttg
      841 aagcaaagtt ggtagagaat ggtattcttt atgaatttgt tgaaccacaa aaagaatttt
      901 atgattttag cgaagatatt ttgtctgata taattgcaga aggctacgat aaagatgaga
      961 ttcttgtaga atttaagaat agaaaaaaca aaatgcattc ttcctttaga gatattgctg
     1021 aagatacact tactaattct aaagtaatga ctaaagagga gttggcaaca gaaattggct
     1081 tatgaaatcg ttccttcaaa acatgtc
//
LOCUS       DACDRN010000181         1090 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDRN010000181 DACDRN010000000
VERSION     DACDRN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.191"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            61..918
                     /locus_tag="IWJ80_002876"
     CDS             61..918
                     /locus_tag="IWJ80_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9462524.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
BASE COUNT          413 a          157 c          232 g          288 t
ORIGIN      
        1 aaaaataaat atgttataat agtatcaaat gaatattgct aaaagaaaag aggtaggaac
       61 gtggtagcga agaaatatgt aatcggtaat tttaaaggtg gcgtaggtaa aagtacgtgc
      121 gcgcaaatgt tagggtttga atcagcagtt aatgaaggaa aaagaacctt gataattgac
      181 ttggatatgc aaggaaatac ttctgatgtg atgaacttaa cgcatatgaa cttcactaac
      241 gaagaaggag gcggagaagg tgaaccgatt gtttacgaaa atactgtgac tgacgtgttg
      301 ataaacaatc ttccacctaa agaggctgtc tataaagtaa tcaataacct ctatatactt
      361 ccagcagaca tgtcctttga aatgtatgat gattggatca aagaccaata taatgattca
      421 ttatcccaat tcaaatatat gaaagaaaag ctagaaccat tatttgatga gtttgacgtc
      481 atctatttag atgtcccacc ttctatttct gtatatagta aatccgcgat gtacattgcg
      541 gattgggcaa tcgtcgtctt acagacccaa gtaaaatcga tgagaaatgc gatgcaatat
      601 cttgagtata tggacttttt tgtgaaagaa tttgatacaa atttatatgt tgctggtgtt
      661 attcctttca tgttggaaag cggtgacgca gtagatcagg aaatgtataa acaagcccaa
      721 gagatttatg gggaacattt gattaaaaat gttgtactca aaaatgcgcg cttaaaacga
      781 tacgacggat caggtattac gatggagaag acaaagaaag gtctcttaaa acaatgggat
      841 cgcaaaagcc acgaattatt tatcagtatt ttgaaagaat tggaagaaca cgagaattgg
      901 tatgcggagg tggagtaatt ggctgacatc aaaggacttg atatttttgg gaacaacaaa
      961 ggactcgcca aaataaaaaa agaagcagaa gcaaagaaaa agcagcaagc aattacacca
     1021 tctgaaaata agcaattaga agtgcaccaa gttcaagaaa taaaaacttc ctccaatgtt
     1081 gaaagacaga
//
LOCUS       DACDRN010000182         1089 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDRN010000182 DACDRN010000000
VERSION     DACDRN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.192"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            66..851
                     /locus_tag="IWJ80_002877"
     CDS             66..851
                     /locus_tag="IWJ80_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP9462525.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT          427 a          163 c          177 g          322 t
ORIGIN      
        1 cccttttaga aaaagcaaaa aaaaaagaaa aaatcttatt cttgataaca agctacgtga
       61 cgtatatgcc taacaaaaat agaacttttg aatcaatctt aaatgaaggt ttaaccacag
      121 aaccggcatc cattattttt gatgaagtta tcgatgacgt tgaagatagt aagtatcata
      181 aaaatttaaa agaacttttt tattttgcac tcaacaatgg accaagtttg ttcactggta
      241 tatcagtacc tgatagtgat aatccattaa ttatggcaaa aatttactta acaaaatggt
      301 gtaataaata catcagggat aggaataacc cagcattaaa aaaacctctt aaaacatttg
      361 gcgaaaaaga tgctgcttta actactagag tagcagcaaa tgctaatatt gatgatcaaa
      421 aagtattgaa tgactacttg cgtggacatt ttttatatat gagcgctgag aacatgaacg
      481 gaagtatttt agaggaatat ttagccgaag tgttagaacc tgaaggttgg atttggtgcg
      541 ccggctctgt atatcgagct gttgatttct gttatttagg aacctcacca attcttttac
      601 aagtgaaaaa taaatacaat acagaaagca gttcgagttc agctataaga gtaggaacaa
      661 caattagaaa atggaatcgt ttaaataagt cgacaaaaat ttctggtttg gatagcccta
      721 tccccaattg gaaagcatta atagaaatga ctgaagctag taaggaactg gcagctaagt
      781 taactgaaaa tagttattta gcttatatta atgaaaaatc cacacgagaa ttatggacac
      841 tcgatgacta acaatatcct tattagtatc tatccacgat acatggaaaa aattataaac
      901 aaacaaaaaa actttgaatt tagaaatttc tatcttacct cagatcctta taatatcttt
      961 tggatttacg aaacaatccc caccaaagct ttaaagtata aattgattgt aaaaaatcca
     1021 attactaaac ttaagaaaaa tcaacactac tttttaggag acgatgagtt tgaacaatta
     1081 gtttaaaag
//
LOCUS       DACDRN010000183         1086 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDRN010000183 DACDRN010000000
VERSION     DACDRN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.193"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..688
                     /locus_tag="IWJ80_002878"
     CDS             <1..688
                     /locus_tag="IWJ80_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP9462526.1"
                     /translation="YDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGD
                     TIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGI
                     VYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEM
                     HITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQL
                     GLVDNFNNIS"
     gene            833..>1086
                     /locus_tag="IWJ80_002879"
     CDS             833..>1086
                     /locus_tag="IWJ80_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462527.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSE"
BASE COUNT          400 a          116 c          195 g          375 t
ORIGIN      
        1 ttacgatcca gaaatatatc tgtttcttta tgaaaataaa atactggaat atgttaaaaa
       61 tggagatatt cgaaatttag aaaacatggt ttttaatctg agtaatggaa taattccaag
      121 tgttagtgga gatacgattc ggtctgaaaa aaattattct atcattgttt ttgaaaagtt
      181 agcacaaacg tctataacat taggaatgga tataatagaa gcgtatcaaa gtagagatgc
      241 tttgatacaa gaaaacgaat tagctgtatc gttacctgag gtcttaaaag tgagggattc
      301 tgggattgtt tattatacta aggaaatcgg aaaaactaag atagagcatc tttctccctt
      361 aatttcttct gtcgttcaat tcatcggctt aaatatctat aaaagaatta ctgtaaagga
      421 aatagctaat tatttttctg taagtgaaac taaattaaga gaaagcttta agaatgaaat
      481 gcatattacg atttataatt atatctctaa gcgaaaaatt tctacggcta aaataatgtt
      541 gaaatcaaac catactatca gtgaagtttc tttaggttta ggattttcag attcttcaca
      601 cttttcgaga gtattcaaaa agtacgcagg tgtatctcct aagcaatatc aattaggttt
      661 agtggataat ttcaacaaca ttagctgaga ggttttattt tattatgaga ctatatcatt
      721 tacgtagttt ggaggagttg aggaaattgg attttaacaa aaaatttttt gggaaatttc
      781 tgatttgagt tgtttcgtag aatctgagcg tctaagtaat ggtaataata gtgtgttaaa
      841 tgaatatgac aaagtagtaa ttaataaagt aaaaagttta tgtgaagaag ctagagaatc
      901 tatcagtcat attaatggta gcaattttaa gcaaacatta tttaatctga ttaagctaga
      961 tgctaaaata tcaagttatt tattcttttt aatacatgat gaatttatgg attatcaaaa
     1021 attggatcag ttaattgaaa gagaaagctg ggaagattat tacgtgggtc taactttttc
     1081 agaaaa
//
LOCUS       DACDRN010000184         1062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDRN010000184 DACDRN010000000
VERSION     DACDRN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.194"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(36..992)
                     /locus_tag="IWJ80_002880"
     CDS             complement(36..992)
                     /locus_tag="IWJ80_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462528.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          178 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca ac
//
LOCUS       DACDRN010000185         1059 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDRN010000185 DACDRN010000000
VERSION     DACDRN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.195"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..345)
                     /locus_tag="IWJ80_002881"
                     /pseudo
     CDS             complement(1..345)
                     /locus_tag="IWJ80_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            421..576
                     /locus_tag="IWJ80_002882"
                     /pseudo
     CDS             421..576
                     /locus_tag="IWJ80_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            595..>1059
                     /locus_tag="IWJ80_002883"
     CDS             595..>1059
                     /locus_tag="IWJ80_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9462529.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVH"
BASE COUNT          374 a          128 c          191 g          366 t
ORIGIN      
        1 tatttagaat tttgcacatc gctgatatgg aatatttatg cttgttggca tcaattactt
       61 gtcttttcgt ccgaatatca gcgccgcttg ctttaaaata tcattctcca tttcgagttg
      121 cttatttttc tttcttagtg cgatcaattc tgcttgttct ggtgttaagt tgtctctttc
      181 tttgaatgag cccgttgatt gtgcttgctt catccattta tcgaaagaag aaggcgtaag
      241 ctcatactct cgaataattt ctgcgcgagg cttaccagcg agatagagat cgacgatttg
      301 ttgtttgaat tcttttgaat aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt
      361 gtgtttttta ttattctaca caccttattt tttctgtcta gtctagtgta gccgattcag
      421 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
      481 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      541 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      601 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      661 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      721 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      781 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      841 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      901 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      961 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
     1021 aatatagaag aaaatgataa gaattcaatt attgttcat
//
LOCUS       DACDRN010000186         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDRN010000186 DACDRN010000000
VERSION     DACDRN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.196"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1035)
                     /locus_tag="IWJ80_002884"
     rRNA            complement(<1..>1035)
                     /locus_tag="IWJ80_002884"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACDRN010000187         1027 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDRN010000187 DACDRN010000000
VERSION     DACDRN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.197"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            192..>1027
                     /locus_tag="IWJ80_002885"
     CDS             192..>1027
                     /locus_tag="IWJ80_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9462530.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VD"
BASE COUNT          363 a          176 c          192 g          296 t
ORIGIN      
        1 aaaccaaaat aaaaatttgc atcatcaaaa aagtgaagat gtgatagaat gaggactggt
       61 tcactttcac ttaactaata gatggaattt ctttataacg ccgattgtaa aattaagcta
      121 gacaactaaa aaagtcattt gtgctacact caaaatagtt ccgtccaaag aattataagg
      181 agtgagtaca aatgacctat acccatctta caacggatga acttgtaatg atagagtctt
      241 atttcaaaat aaatcaatct gttgcgaaaa ctgcgcattt cttgaatcgt tcaagacaaa
      301 cgatccataa agtatacctg ttcttcaagc aaggaaaatc agccttagag tactatcaac
      361 agtataagaa aaacaaatca aactgtggta gacgcccgct tgttttacct gaggaacaat
      421 cagaatatat tcaaagaaag gttgttcaag gatggacacc cgatgtgatt gttggtcgtg
      481 cagcgtttcc tattcgttgt tctgctcgta ccatttatcg tatgttcaaa aaggggctat
      541 ttaatccttc tgacttaccg atgaaaggca agcgtaaacc aaatggacat caagaaagac
      601 gtggaaaaca agctttccgt cgctctattc atgaacgtga aaaagattat agccaattct
      661 caaatgagtt tggtcacctt gaaggtgaca ctatcgtagg tttgaaacat aaaagtgctg
      721 taattacctt agttgagcga ttatcaaaag ttatcatcac attgaaaccg tgtggtagac
      781 aagcgattga tattgaaaac aaattaaatc attggtttga atctgtaccg aaaaatctat
      841 tcaaatccat cacttttgat tgtggaaagg aattttcaaa ttggaaacag atcagtaatg
      901 ccaatgatat tgccatttat ttcgctgatc caggaacgcc gtctcaaaga ggcctaaacg
      961 agaattctaa cggattgtta cgtagagatg gtttattgaa atctatggat ttcaattcag
     1021 tagatga
//
LOCUS       DACDRN010000188         1003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDRN010000188 DACDRN010000000
VERSION     DACDRN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.198"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>1003
                     /locus_tag="IWJ80_002886"
     CDS             <1..>1003
                     /locus_tag="IWJ80_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP9462531.1"
                     /translation="PQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDAFALGKYHTGS
                     NLCLWKACSGLAEIFEALEQLSLSERYKDIACKLKESICKQMTIDGTFGEQFLEGIGD
                     EEKTTYSVKNYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMPFYQFLDNKDP
                     LYQNTMTFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSDMHNHQAFVTRLEEL
                     ICLADLDGSWWWWPYRLEAKQGEVVRDFGCGKCGWASGLFVSIMISQYFGLKFKKGVL
                     QIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKKILHVEKGKTI
                     "
BASE COUNT          305 a          169 c          228 g          301 t
ORIGIN      
        1 tccgcaggtt ctactgaatg cttcaagaat tattgaggac atattagctc aacgtcggtc
       61 aggggaacca atgctttttc catctgtttg gttatcagac gcctttgcac ttggaaagta
      121 tcataccggc agcaatcttt gtctttggaa ggcctgtagt ggacttgcag aaatttttga
      181 agccctagaa caactgagtc ttagcgagcg atacaaagac atcgcctgta aactaaagga
      241 atcaatttgt aaacaaatga ccatagatgg tacttttggg gagcaatttt tggaggggat
      301 cggtgatgag gagaaaacaa cctattcagt gaagaactat caaaaaccga ttcttgagca
      361 agggttaatc tttttaagtg atgtgattgt ggatgggaaa atcaatttat tgatgcatga
      421 tggagaagaa tcagacacta cgttgatgcc tttttatcaa tttttagata ataaagatcc
      481 tttatatcag aacaccatga ccttttctgc tagttctttc aatccaactt atagcgagga
      541 aatcaaaggc atcacttggg gattagaatc cggcgccact tttcctggct ttatcactgt
      601 tttaatgagt gacatgcaca accatcaagc cttcgtaacc cgattagagg agctgatttg
      661 tttggcagat ttagacggtt catggtggtg gtggccgtat cgtttagagg ctaaacaagg
      721 agaagttgtg cgagactttg gctgcggcaa atgcggctgg gcatcgggcc tttttgtcag
      781 tataatgatc agtcaatatt tcggattgaa attcaaaaaa ggagtattgc aaattgatcc
      841 tgttgataat ttaacgtttt cttggaaaaa tttgaagttc ggtcaagcct tcatttcgat
      901 tgaatgtacg caatccgagt tgagtatttc aaatttgtca gaaaagccat tgaaaataag
      961 tgatacgaaa aaaattcttc acgtagaaaa gggaaaaacg att
//
LOCUS       DACDRN010000189          988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDRN010000189 DACDRN010000000
VERSION     DACDRN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.199"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..927
                     /locus_tag="IWJ80_002887"
     CDS             <1..927
                     /locus_tag="IWJ80_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP9462532.1"
                     /translation="LTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
BASE COUNT          333 a          196 c          183 g          276 t
ORIGIN      
        1 ctgactgaaa aagtatcttt atcttttatt gcgaaaagct gtcaggtatc tttgaccacc
       61 gttattcgta cattgaaaga gtttaaaagc tatttaccaa agcaatctaa gaagatcctc
      121 cccagagtat tgatggttga cgaatttcgt tcgcatgctt ccatagaaga taagatgagc
      181 tttatttgcg cggatggcga aacaggaaaa ttaatagatg ttttgcctac gcgtaaatta
      241 cctcgattaa caagctattt cttaggttgt accaatccag aagaagtaga attcttggtg
      301 acagacatga acgccgccta cttccagctc accaaacgtg ttctgccaaa tgcgaaagtc
      361 gtgattgatc ggtttcatat tgtcaaacac atgaatcaag cgttcaatga gttgcgtatc
      421 cgtgaaatga atgaacttcg taaagccgga cagaaaagcc aggcagaaaa actgaaaaag
      481 aactggcgct ttttgctaaa aaatcgtgca aacatcaacc attatgaata caaaacatgg
      541 aaaagtttcc gagcaccaaa atacccattt cttactgaag caatgatgat tgatcgattg
      601 cttgagtttt ctccgcccct aaaggaggcg tatccctttt ttcatgaatt agttgaagcc
      661 tttcgagaca aagaccctga cttatttttc tccttattgg cagaacttcc cgaaacgtta
      721 gatgacagct ttcgggaaaa gcttcaaaac cttctgacct atgaagaagg catcaccaac
      781 gcaatgatct atccttattc caatggaaaa atagaagcga agaataccca cataaagaca
      841 atgaaacgag tatcctacgg atttaaatca tttgagaaca tgagaattag aatctttttg
      901 atcaatcaat taatcaatgt aagataacaa aaaatctgag ccagaatgtg gttcattctg
      961 actcagattt gattttgcac aactcatc
//
LOCUS       DACDRN010000190          925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDRN010000190 DACDRN010000000
VERSION     DACDRN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.200"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..518)
                     /locus_tag="IWJ80_002888"
     CDS             complement(<1..518)
                     /locus_tag="IWJ80_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP9462533.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPS"
     gene            complement(569..>925)
                     /locus_tag="IWJ80_002889"
     CDS             complement(569..>925)
                     /locus_tag="IWJ80_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9462534.1"
                     /translation="LMRYYGGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGA
                     MTISMVKFNTVLSFSMKGEELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKII
                     FGMVVLGILLSLLGIA"
BASE COUNT          293 a          194 c          150 g          288 t
ORIGIN      
        1 tacgaaggca catctttcac tgaaacttga aggctttcgt gtgtttcatt cacaatgtaa
       61 acctgttgaa caagcatact taaacgctta ttagccagta tgggagcaaa ggatactaca
      121 atgaccttaa acgtcgaata atgatgaata tatctaggca aacagtcttg aatcacatcg
      181 acttcaatac gatgatccga ttctgacaaa tggtagacaa tcccgtttac ttcaaccttt
      241 aaggataacg aattggtttg agagagctgt ttattgaccc aaacacctgg tttttcctca
      301 gttagtaaat tttctcgata aaaggtcaac gtttgaatcg tcccgcaaac atcaaggttt
      361 gctaatgtct tttgattact tacatcgaag gtcaatccct ttacatcagg aatattccga
      421 tagtagctgt attcgtttga agtagtcaca tttccgtcgt ttaactcagc attaaaaaat
      481 gggtgtttgg gtgaataaat tacttcatta atagtcatct tttccctcct tgctaaaaaa
      541 actgcagcct agtagctgca gcttcctact atgcgattcc aagtaatgac aataaaattc
      601 ccaagacgac cattccaaaa ataattttga tgacgccgac ttttttatct agtaatttga
      661 aacagctcaa tgttaccaat aacggtacaa ttcctttaaa aatactatcg aggatttctt
      721 ggacattcat tagttcttcg cccttcattg aaaagctcaa aacggtattg aatttgacca
      781 tactgatcgt cattgcacct accatgatca gacccaagat actagcagct tttgtcagaa
      841 tttcaattaa cccgttcgca tacagctttt caatatattt agagcctaat gaatagccta
      901 aaacgccgcc atagtatcgc atcaa
//
LOCUS       DACDRN010000191          922 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDRN010000191 DACDRN010000000
VERSION     DACDRN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.201"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..355
                     /locus_tag="IWJ80_002890"
     CDS             <1..355
                     /locus_tag="IWJ80_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462535.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..922
                     /locus_tag="IWJ80_002891"
                     /pseudo
     CDS             615..922
                     /locus_tag="IWJ80_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          353 a          108 c          195 g          266 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gc
//
LOCUS       DACDRN010000192          846 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDRN010000192 DACDRN010000000
VERSION     DACDRN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.202"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            69..644
                     /locus_tag="IWJ80_002892"
     CDS             69..644
                     /locus_tag="IWJ80_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9462536.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(713..>846)
                     /locus_tag="IWJ80_002893"
     CDS             complement(713..>846)
                     /locus_tag="IWJ80_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9462537.1"
                     /translation="NQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT          235 a          144 c          181 g          286 t
ORIGIN      
        1 cgttctttta aatgcacaag aagaaacgat tatgaaagat ttggaaaggt taaagatggg
       61 ctaacaaaat gaataaaaac aaaatggcaa ctcgcgtact tacagtgatg gctttgtgta
      121 ttgggatcaa ttatattgga ggaacgattg ctttatggct tcgtttgcca atctatctcg
      181 attctatcgg aacgattttt gctggtgcat tgcttgggcc tgtacctgga atgctgacag
      241 gattgtccag cggtatttta agtggagtga ctacagatat tttttctctg tattattcgc
      301 caatccaaat tctcacaggc ttgcttgctg gattgatttt gccaagatat ctcggcaaag
      361 ataaaaacag cttgaaactg ttattactcg ctttgatttt gtcggtacct ggaacgatcg
      421 tttcttcgat cattaccgtt cagttatttg gcgggatcac ctcctcagga tccagtatga
      481 tcgttcagct attacatggg ttaggtttaa gtcagacaac aagtgtgatc ttggtacagg
      541 cgggtacaga ctaccttgat cgcttgttgt ctatcgtcgt tgtttcatta gtcatcacta
      601 tgttgcccca acgcttgata tattcaaaaa gaaaaatagg ctgaataaac agagctggat
      661 tagcaaaata atgctaatct ggctctgttt tttcatttta ttatttgtca aattaagcga
      721 gacaaaactt tccatttacg taactcaaaa aaacttctaa tggtgtttgg taatttagtg
      781 attttctagg gatatggttt cttttggacg caacggatga gatgaatcct tgattgactt
      841 ggttga
//
LOCUS       DACDRN010000193          846 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDRN010000193 DACDRN010000000
VERSION     DACDRN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.203"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            11..>846
                     /locus_tag="IWJ80_002894"
     CDS             11..>846
                     /locus_tag="IWJ80_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9462538.1"
                     /translation="MTYTHLTTDELVIIESYFKINQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VD"
BASE COUNT          290 a          147 c          162 g          247 t
ORIGIN      
        1 gtgagtacaa atgacctata cccatcttac aacggatgaa cttgtaataa tagagtctta
       61 tttcaaaatt aatcaatctg ttgctaaaac tgcccattgc ttgaatcgtt caagacaaac
      121 gatccataaa gtatatctgt tcttcaagca aggaaaatca gccttagaat attatcaaca
      181 gtataagaaa aacaaatcaa actgtggtag acgtccgctt gttttacccg aggaacaatc
      241 agaatatatt caaagaaagg ttgttcaagg atggacaccc gatgtgattg ttggtcgtgc
      301 agcgtttcct attagttgtt ctgctcgtac catttatcgt atgttcaaaa aggggctatt
      361 tgattcttct gacttaccga tgaaaggcaa gcgtaaaccg aatggacatc aagaaagacg
      421 tggaaaacaa actttccgcc gctctattca tgaacgtgaa aaggattata gccaattctc
      481 aaatgagttt ggtcaccttg aaggtgacac tatcgtaggt ctgaaacata aaagtgctgt
      541 aattacctta gttgaacgat tatcaaaagt tatcatcaca ttgaaaccgt gtggtagaca
      601 agcgattgat attgaaaaaa aattaaatca atggtttgaa tctgtaccga aaaacctatt
      661 caaatccatc acttttgatt gtggaaagga attttcaaat tggaaacaga tcagtaatgt
      721 caatgatatt gccatttatt tcgctgatcc aggaacgccg tctcaaagag gcctaaacga
      781 gaattctaac ggattgttac gtagagatgg tttattgaaa tctatggatt tcaattcagt
      841 agatga
//
LOCUS       DACDRN010000194          836 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDRN010000194 DACDRN010000000
VERSION     DACDRN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.204"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>836)
                     /locus_tag="IWJ80_002895"
     rRNA            complement(<1..>836)
                     /locus_tag="IWJ80_002895"
                     /product="23S ribosomal RNA"
BASE COUNT          183 a          261 c          188 g          204 t
ORIGIN      
        1 tctctcaggg atcttacttt cttaaagaaa tgggaaatct catcttgagg tgggcttcac
       61 acttagatgc tttcagcgtt tatcccttcc ctacatagct acccagcaat gcccttggca
      121 gaacaactgg tacaccagcg gtaagtccat cccggtcctc tcgtactaag gacagctcct
      181 ctcaaatttc caacgcccgc gacggatagg gaccgaactg tctcacgacg ttctgaaccc
      241 agctcgcgtg ccgctttaat gggcgaacag cccaaccctt gggaccgact acagccccag
      301 gatgcgacga gccgacatcg aggtgccaaa cctccccgtc gatgtggact cttgggggag
      361 ataagcctgt tatccccagg gtagctttta tccgttgagc gatggccctt ccatgcggaa
      421 ccaccggatc actaagcccg actttcgtcc ctgctcgact tgttggtctc gcagtcaagc
      481 tcccttctgc ctttacactc ttcgaatgat ttccaaccat tctgagggaa cctttgggcg
      541 cctccgttac cttttaggag gcgaccgccc cagtcaaact gcccatctga cactgtctcc
      601 caccacgatt agtggtgcgg gttagagtgg ccataacgca ggggtagtat cccaccagcg
      661 cctccatcga aactagcgtt ccgatttcta cggctcctac ctatcctgta catgcggtac
      721 agacactcaa tatcaaacta cagtaaagct ccatggggtc tttccgtcct gtcgcgggta
      781 acctgcatct tcacaggtac taaaatttca ccgagtctct cgttgagaca gtgccc
//
LOCUS       DACDRN010000195          832 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDRN010000195 DACDRN010000000
VERSION     DACDRN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.205"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..180
                     /locus_tag="IWJ80_002896"
     CDS             <1..180
                     /locus_tag="IWJ80_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9462539.1"
                     /translation="LDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELS
                     GKKLKKVNKMNLEVE"
     gene            180..>832
                     /locus_tag="IWJ80_002897"
     CDS             180..>832
                     /locus_tag="IWJ80_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462540.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     "
BASE COUNT          360 a           81 c          154 g          237 t
ORIGIN      
        1 ctagacagtg ttactgctgg agaaataatt aatatattaa agacattagc taaagataga
       61 aataaatgtg taatagttgt aacacatagt aaggaagtag cagattctgc ggatatcatt
      121 ttagaactaa gtggtaaaaa gttgaaaaaa gtaaataaaa tgaatttgga ggttgaataa
      181 tgataaaaaa tgcatttgct tatgtaacta gaaaaagttt aaaatcatta attatcatat
      241 tagttatttt atctatgtca actttaagtc tcattagttt atctattaaa gatgctacgg
      301 acagagcttc aaaagaaaca tttgctaata taacaaatag tttttctatg gagataaata
      361 gacaagtaaa tccaggaaca cctagaggtg ggggaaatgt aaaaggtgaa gatattaaaa
      421 agatatctca aacagatagt atagactcct atgtaaaaag aataaacagt gttgcagatt
      481 tagttgatta tgatattatt gaaactcaag agactcttgc gaatcaatca cctgaaagag
      541 caaagaattt taaaagaaca gttatgttaa ctggggttaa cgactcaaca aaagaaacaa
      601 aatttgtatc agaagcatat aaattagtag aaggaaagca tttagaaaat gaagataaaa
      661 ataaaatctt aatgcataaa gatttagctg aaaaaaataa ccttaaagtt ggagataaga
      721 taaaaataaa atctaattta tttgatgctg ataatgagaa aggtgcagat gaaacagtag
      781 aagtagaaat taaaggtcta ttcgatgggc ataatagcgg tgtagtaagt gc
//
LOCUS       DACDRN010000196          826 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDRN010000196 DACDRN010000000
VERSION     DACDRN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.206"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..586)
                     /locus_tag="IWJ80_002898"
     CDS             complement(<1..586)
                     /locus_tag="IWJ80_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462541.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKIND"
BASE COUNT          277 a          131 c           93 g          325 t
ORIGIN      
        1 tatcattaat tttataaaaa aaattaaatg tttttagcac tgccatataa cattcaaaac
       61 attcttctat agttggttta cttccttgag gagaatgaac caatttatta cgtttttcta
      121 tgaaattttt aatttccttt cttatttctt tcggaaactc aatattaaag taccctttaa
      181 acatgctccc taataatttg tctacagggg gagattgtaa attctcaaat attagagtta
      241 aatcaggttt ctcttgttga taaaattcct ttacccctat ttcacaggct gtaattatcg
      301 aaataaacct atctctagta tttgatatat ttttacttct tttaaggagt ataagagaaa
      361 aggggatact ttcgtgtcca tgcatgtaat taactaattg ttgagcttca gaagttatat
      421 ctctaattat atagttaacg gaattaggaa gtgtctttaa ctctaatgaa atttctatcc
      481 aattactatc ttctaatctg tatatgctcg aagtataatt tgattcgtca gcaagcatcg
      541 ctacatccca aacactgatc ataaaatctt ttaaacgttc aatcatttca gcaatattgc
      601 taaaatcagc taatttttct ttagaataaa aaaaaacatt tatatcagaa tcaataaatt
      661 ctattttttt attactatat cgtcatcatt atcttgacca tttatgttta ttgttccctg
      721 agtggggagt acttctttca aattcaagtt aaatttatat tccattactt ctcctccaat
      781 atataaaaat tacttaactg tatccaaatt taatatgtaa ataaat
//
LOCUS       DACDRN010000197          773 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDRN010000197 DACDRN010000000
VERSION     DACDRN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.207"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..370
                     /locus_tag="IWJ80_002899"
     CDS             <1..370
                     /locus_tag="IWJ80_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9462542.1"
                     /translation="LILILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIFISIPSII
                     SSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQSYLILISIIVLSV
                     VFASSLMLIKKPKEILSKIS"
     gene            380..>773
                     /locus_tag="IWJ80_002900"
     CDS             380..>773
                     /locus_tag="IWJ80_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9462543.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLS"
BASE COUNT          320 a           73 c          115 g          265 t
ORIGIN      
        1 attaatcttg attctatggt taagggaaag aatttatgaa ataggtatat tcttatctat
       61 tggaacatct aagatacaaa ttataatgca atttatattc gagttaatat tcatatcgat
      121 accaagtata atatcttcct tatttttagg aaatgtatta ctaaaagtaa ttgtagatgg
      181 attcattaac tcagaggatt caatgatttc cggtggaagt ttaataaata atagtagttt
      241 tatgtcaaat ataacaacac ttggacaaag ttatttaata ttaataagta ttattgtttt
      301 atcagttgta tttgcttctt cattaatgtt aatcaagaag cctaaagaaa tcttatcaaa
      361 aataagttag gagaaaataa tggatatatt agaaataaag aatgtagcat atagttatgc
      421 aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa tttgaacttg ggaagtttta
      481 tgcgatagta ggaaagtcag gaacaggaaa atctacactt ctttccttac ttgctggact
      541 tgataaacct caaacaggaa aaatattgtt taagaatgaa gatatacaaa ataaaggata
      601 tagtaatcat agaaaaaata atatatcttt agtatttcaa aactataatt taatagatta
      661 tttatcacca attgaaaata ttagattagt aaataaatca gcagatgaaa gtatcttgtt
      721 tgaactaggt ttagataaaa aacaaataaa aagaaatgtt atgaaattat ctg
//
LOCUS       DACDRN010000198          748 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDRN010000198 DACDRN010000000
VERSION     DACDRN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.208"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            64..687
                     /locus_tag="IWJ80_002901"
     CDS             64..687
                     /locus_tag="IWJ80_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9462544.1"
                     /translation="MKIAYIRVSSIDQNEQRQIEEMKKFGAEKIFIEKQSGATITHRP
                     IFQEALEFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PHLDKFIKDLIIQILAMIAEQERTESKRRQAQVIKIAKANGVYKGRPKLYSADTKNPQ
                     RRLVYENIVKDLNEGIAISKIAKNYNITRQTIYRIKNDIEYNSNQMN"
BASE COUNT          284 a          120 c          129 g          215 t
ORIGIN      
        1 tataaaaaaa ttagtgttga ttttgagtgt attttaaata gtttgaggaa gtaggtaaaa
       61 attttgaaaa ttgcttatat tcgagtttcg tcgattgatc aaaatgagca acgccaaatt
      121 gaagaaatga aaaagttcgg cgcagaaaaa attttcatcg agaaacaatc tggagcaacg
      181 attactcatc gaccgatttt ccaggaagct ttagagtttg tccgagacca agatattttt
      241 attgtcgaag caatcgaccg acttggaaga aattacgacg aaatcattgc ttcagtcaat
      301 tacctaaaga agaaaaacgt gcagctgatc atcaccagtc taccgatcat ggctgaagca
      361 attggcaatc ctcatttgga taaattcatt aaagatttaa tcatccaaat tttggccatg
      421 attgcagagc aagagcgtac cgaatctaaa cgacgccagg cgcaagtcat taaaattgca
      481 aaagcaaatg gcgtctataa aggtcgtcca aaactctata gtgcagatac aaaaaatcct
      541 caacgacgtt tagtttatga aaacatcgtt aaggatttaa atgagggcat agctatttca
      601 aaaattgcta aaaactacaa tattactaga caaactattt acagaattaa gaatgatatt
      661 gagtataaca gtaatcaaat gaattaaaaa agttctttat acattaaaat ttcttctttt
      721 aactcatcaa ttctaggaga tctaacat
//
LOCUS       DACDRN010000199          708 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDRN010000199 DACDRN010000000
VERSION     DACDRN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.209"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..368
                     /locus_tag="IWJ80_002902"
     CDS             <1..368
                     /locus_tag="IWJ80_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9462545.1"
                     /translation="QFDAYCKKVLRNEAKSIRKRNANIKDNEEPLADSNEGRYSQTHF
                     DEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDAEIGKILNMSTSG
                     IWYQRKKAVEQLKMGYDLW"
     gene            362..607
                     /locus_tag="IWJ80_002903"
     CDS             362..607
                     /locus_tag="IWJ80_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9462546.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
BASE COUNT          264 a          100 c          122 g          222 t
ORIGIN      
        1 accaatttga tgcctactgt aaaaaagtat tacgaaacga agctaagtct attcgaaaac
       61 gaaacgctaa cataaaggat aatgaggaac cattggcaga ttcaaatgaa ggcaggtatt
      121 cccaaactca ttttgatgaa caagatgtct atttcttatt tggaatggaa atattaattt
      181 cagatcaaaa gttatcagca gcaattgatc agttatcgga gataagaaga aagattatcc
      241 tactttatta ttttgcaggt tttaatgatg ctgaaatcgg taaaattctc aatatgagca
      301 caagtggtat ctggtatcaa cgaaagaagg cagttgaaca attgaaaatg gggtatgacc
      361 tatggtagat aaatctaaaa agcggcagta cctattagtt ccactatcca ttatcttatc
      421 tgctctagaa ggagatccaa tttcgatcaa gatgatactc aaacattatc attcttatat
      481 ggctaagtta tgtttgacaa atggatttaa cgaggtaggt caattcgtta cctatgtaga
      541 tgaatatatg ataagtcagc tagaaattaa attaatagaa gcgatcttaa aatttaaaat
      601 taactaatta aaaaaatccc ctttccactt tttctgttta aaagaatctt ctaatagtta
      661 aggaattagt agattctttt aggtagaaaa cctattgttc attgaaaa
//
LOCUS       DACDRN010000200          640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDRN010000200 DACDRN010000000
VERSION     DACDRN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.210"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(82..495)
                     /locus_tag="IWJ80_002904"
     CDS             complement(82..495)
                     /locus_tag="IWJ80_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAP9462547.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          220 a           83 c           72 g          265 t
ORIGIN      
        1 tttttaggaa tgtaaagtct tatctctttt cttaattgta caattagtat tatctgcact
       61 actttctaat taaataaatt tttagttatt aacttgatta aaatcagaga taatatcatt
      121 taattcagtt tgaagggttg taacgattaa atggaaattt tcttcatctg ttttattata
      181 tggggatgtt ttcgttatat ataacaactt ttctttttca attttcatta ataactctgt
      241 cgaagtctgt actttatctt gataattaaa tgcagacata ccactttgta taattgtaat
      301 tattgctgaa gcaattgaaa caatcaatag taaagacctg tgttcggaag cctgatcaat
      361 taatattggg atactagctg ataagactat ttttataata tttccgatac caattactct
      421 attataaaat cgaatttctt tttttaatct atcgattgta gaatcaatag attcgataaa
      481 cgctttttga tccatcattt tctctccaat cattttatta aaatcattaa caaaaatatt
      541 ataataggat ttaagtaagt tctaactaat cacatataaa aataaaaaaa catttgtatt
      601 ttatcaaagt tttattatac ttgtcaattt gtgtacatcc
//
LOCUS       DACDRN010000201          617 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDRN010000201 DACDRN010000000
VERSION     DACDRN010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.211"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..614)
                     /locus_tag="IWJ80_002905"
     CDS             complement(<1..614)
                     /locus_tag="IWJ80_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014096946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9462548.1"
                     /translation="MPFQTSAKITIENLHPKDMSSFFYTINYALVDELEENSLYFHAN
                     WHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLERYWWGEGEFKFYIDGDSEYPTV
                     TSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYPYQSNRDATRDRFETGKLNAVH
                     AFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGNDDVRLFER"
BASE COUNT          177 a          140 c          122 g          178 t
ORIGIN      
        1 tgccgctcaa ataagcgaac atcatcgttg ccgatttgtt gtaaagtgac cgataaatct
       61 tcggtaaaac agatggggtc ttgaagatgc caacgataaa gaccatgtcg tggcattgcg
      121 tcgtcaccaa aggcatgaac ggcattcaat tttcccgttt caaaacgatc tcttgtggca
      181 tctctatttg attggtaagg ataccctaaa aaggctgttg aatagttttt cgcatgtgga
      241 cgtccgaact catcgtactc atgaaaggcc cacgccccgc caaaataatc ttctgaacct
      301 gttgaggtta cggtcggata ctcactatcc ccatcaatgt agaatttaaa ctcgccttcg
      361 ccccaccaat aacgttctag gcaggtaagc gcaaggtaag tacccacgta atagccttga
      421 ccctttaatc gatcaattaa cttataatcc tcctgtaatt tggtaatgcg ttcccgatgc
      481 caatttgcgt gaaaatatag gctattttct tccaattcgt ccaccaaagc gtaatttatc
      541 gtataaaaaa atgaggacat atccttcgga tgtaaattct cgatcgtaat tttagcggat
      601 gtttggaaag gcatttc
//
LOCUS       DACDRN010000202          597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDRN010000202 DACDRN010000000
VERSION     DACDRN010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.212"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(164..463)
                     /locus_tag="IWJ80_002906"
     CDS             complement(164..463)
                     /locus_tag="IWJ80_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9462549.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          189 a          109 c           72 g          227 t
ORIGIN      
        1 tactagctgt ttttgtgtta attaaaggat aaaatacaat ataaagaaac atctgtttct
       61 ttatttagaa taaaaaaata cagactctgt tcaaataaat gaacctagcc tgcattatga
      121 gattgactat actttttaat tcattgagat gaataactcg tttttataaa tgcccaaaag
      181 taatcggtgc cgaagccatt cctcgaccta attctttttc aaattttcct attgttgata
      241 tctccatata aaacggtgat actggtttag acaattctcc cctgattgct aacggacgta
      301 aactacgttc caaattattt atgactttag gataatactg acctttttct aagcctgtct
      361 ttgcgtctat caaaagtata cgttcctttt ctttgatctc tggatttaaa acgagatcat
      421 ataattttag taccatgttt tctatttctt cacttttaga catcacgatt tcctcccaaa
      481 aatatatttc ttcccactac attctataaa caaagtatac gctacatcta gattttttta
      541 aatcattact ggattgttaa aacctttcaa tacactgtta tggcaacgat ttttatt
//
LOCUS       DACDRN010000203          576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDRN010000203 DACDRN010000000
VERSION     DACDRN010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.213"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            2..>576
                     /locus_tag="IWJ80_002907"
     rRNA            2..>576
                     /locus_tag="IWJ80_002907"
                     /product="23S ribosomal RNA"
BASE COUNT          184 a          114 c          166 g          112 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatcta
//
LOCUS       DACDRN010000204          556 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDRN010000204 DACDRN010000000
VERSION     DACDRN010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.214"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(152..346)
                     /locus_tag="IWJ80_002908"
     CDS             complement(152..346)
                     /locus_tag="IWJ80_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462550.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYPFV"
BASE COUNT          171 a          113 c           88 g          184 t
ORIGIN      
        1 tgtgatagag aaataacaat ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat
       61 ttgattttga tcgggtctag tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc
      121 ttaaatttca tcttgactta tcaccattag actaaacaaa tggataatct tctgtcgcat
      181 cgacgaacgt cttcgtagtc agatttaacg ttaaaatatc gctactttga taagcttttg
      241 ccgctttgtc tgtgacgaca aaagatctgc ctgtttgaat ttctgtcaca aaaacgagat
      301 tgtctctgct tttttcaaca acaactccaa tcgttgaatg aaccatttct gcttcctccc
      361 ttctctttct tttgatataa tgtagtcgca aatcgcacca ctcaatgagt tggcggaaaa
      421 caattcattg aaaaaaagag tcctggacaa tcacgttcag gactctttta cttcttttat
      481 tcacttaaca actaacactc tgtgagtgaa atggactctt caatgtgatg ttaggatgat
      541 ttcatggaca cgattt
//
LOCUS       DACDRN010000205          528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDRN010000205 DACDRN010000000
VERSION     DACDRN010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.215"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(5..90)
                     /locus_tag="IWJ80_002909"
     tRNA            complement(5..90)
                     /locus_tag="IWJ80_002909"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IWJ80_002910"
     tRNA            complement(107..178)
                     /locus_tag="IWJ80_002910"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IWJ80_002911"
     tRNA            complement(190..262)
                     /locus_tag="IWJ80_002911"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IWJ80_002912"
     tRNA            complement(280..361)
                     /locus_tag="IWJ80_002912"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IWJ80_002913"
     tRNA            complement(369..441)
                     /locus_tag="IWJ80_002913"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IWJ80_002914"
     tRNA            complement(445..517)
                     /locus_tag="IWJ80_002914"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACDRN010000206          514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDRN010000206 DACDRN010000000
VERSION     DACDRN010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.216"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IWJ80_002915"
     CDS             complement(<1..132)
                     /locus_tag="IWJ80_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9462551.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DACDRN010000207          511 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDRN010000207 DACDRN010000000
VERSION     DACDRN010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.217"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>511
                     /locus_tag="IWJ80_002916"
     CDS             <1..>511
                     /locus_tag="IWJ80_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP9462552.1"
                     /translation="QNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTFQESDIE
                     KWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNWGSYPAT
                     NRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSNSLSSAL
                     LASLYFEHTG"
BASE COUNT          146 a           93 c          114 g          158 t
ORIGIN      
        1 caaaatgtgt tgatttctca aaaaggaaat aaggacacga tcgcacaaca ggaagtggca
       61 agcttcgcca ttgcttttgt ggacccaaat gcttttgaag aagtaatgac ttttcaggaa
      121 tctgatatcg aaaaatggct gaaggaaaca ttgaactatt ttgctcagct atttgggcag
      181 ctgtcaatgc caaacgatag agttgtccat ctctatcgtc gtgcacttta tcaaagcttt
      241 tctagtttcg gtatgaatcg acagggacag gtcgtcggca gcaattgggg cagctatcct
      301 gctactaatc gaatttggaa taaggatatg tattacgcat cattgccatt caccatgttt
      361 gaaccggagt tatgtcaaaa aacggttcta tggtttgatc aatatggcgt taagtttcct
      421 ggttcaaaat ttacaggagg tatcaatcat tctctgagta attcgttatc cagtgcgtta
      481 ctggctagtc tttattttga acatactggg g
//
LOCUS       DACDRN010000208          489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDRN010000208 DACDRN010000000
VERSION     DACDRN010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.219"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..414)
                     /locus_tag="IWJ80_002917"
     CDS             complement(<1..414)
                     /locus_tag="IWJ80_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9462553.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKICWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFF"
BASE COUNT          186 a           79 c           62 g          162 t
ORIGIN      
        1 aaaaaacggc tttccaaatg atgaaatacc taaatctaac ttattaagaa agttgaaatt
       61 tttatattca ggaaaccatt tattaatttc atgtgcagca cgtaatgcat tccctggagt
      121 tgtcaaagag aaaattaatt ctgctaagct tattattgaa aatactgata acttataatt
      181 atatcgtcca ttgacaatca aatataaact aactatactt gttagaacaa aaaaacaggc
      241 gttgacttgt tcttgattag ttaaaaatat caataaagga atacttaccc aacatatttt
      301 tctatttatt tcttctcctc ttaagagttg aaaaaacgga taaaaagtta gtaaactaaa
      361 tgctactggc cactgatagt tcaaggtggt tgctctccaa ccggtttccc ccattactgt
      421 caatggtatt aaacagtata tagagcaaac caataataaa tttttaacat ttattttctc
      481 aaaaacata
//
LOCUS       DACDRN010000209          487 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDRN010000209 DACDRN010000000
VERSION     DACDRN010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.220"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>487
                     /locus_tag="IWJ80_002918"
     CDS             <1..>487
                     /locus_tag="IWJ80_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9462554.1"
                     /translation="MIREGISEYLSEFGYTVIQAKDGREALSKFNSDINLVILDIQIP
                     FINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFSLPVLKARIDS
                     LIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLDNNGQVLTRMQ
                     II"
BASE COUNT          197 a           43 c           90 g          157 t
ORIGIN      
        1 tatgataaga gagggaataa gtgaatatct ttcagaattt ggatatactg ttattcaagc
       61 taaagatgga agagaagcct tgtcaaaatt taatagcgat ataaatttgg ttatcttaga
      121 cattcagata ccttttataa atggtttaga agtgttgaaa gaaattagaa agaaaagcaa
      181 tttaccaatt ctaatcttga ctgcatttag tgatgaagaa tataaaattg atgcatttac
      241 taatttagta gacggatatg tagaaaagcc attttcattg ccggtcttaa aagctagaat
      301 agattcttta attaagaaga attatggaca tttagagaag tttgaatata aggatctatc
      361 ggttaatttt aatagctata cagctaaaat aaatggtgaa gaaatagatg taaatgcaaa
      421 agaactagaa gtattaaaat gtttattgga taataatgga caagtgttaa caagaatgca
      481 aattatt
//
LOCUS       DACDRN010000210          453 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDRN010000210 DACDRN010000000
VERSION     DACDRN010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.221"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>453
                     /locus_tag="IWJ80_002919"
     CDS             <1..>453
                     /locus_tag="IWJ80_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9462555.1"
                     /translation="VIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVM
                     QEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLV
                     SIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPE"
BASE COUNT          174 a           57 c           92 g          130 t
ORIGIN      
        1 gttattggtt tggtagggaa aaacggtgtg gggaaaacaa ccttactaag aatactcatg
       61 aagattattc gccctaccaa aggaaagata atcgaaaata aaaaaaattt gtcatcgccg
      121 tttttagtaa tgcaagaaat ggattaccaa tttttcacag aatcagtgag gagcgagtta
      181 gccctgggga atgaaatagt tagtaaagat cagcaagaaa aaattttaaa aaaaatggaa
      241 ctttataaaa tgaaagatca attaccgttt gatttatccg gtggagaaaa acaaagatta
      301 ttggtttcta tagcttcatt gtcaaaaact aatttatttc tatttgatga acctacaagt
      361 gggttggatt atattaatat ggaaaggatt gctgacctta ttaaagactt aaaagaaaag
      421 ggtgcagtag ttatagccac tcatgatccg gaa
//
LOCUS       DACDRN010000211          440 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDRN010000211 DACDRN010000000
VERSION     DACDRN010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.222"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            11..>440
                     /locus_tag="IWJ80_002920"
     CDS             11..>440
                     /locus_tag="IWJ80_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9462556.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          168 a           57 c           88 g          127 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag
//
LOCUS       DACDRN010000212          437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDRN010000212 DACDRN010000000
VERSION     DACDRN010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.223"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            33..>437
                     /locus_tag="IWJ80_002921"
     CDS             33..>437
                     /locus_tag="IWJ80_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9462557.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMS"
BASE COUNT          162 a           83 c           71 g          121 t
ORIGIN      
        1 gacaaccaat tcacagaaaa gaggacttcc ctatgaatga ttttactaca gaaattgtgc
       61 aaactctagt cactaaaggc gatttaaatg aattattccg ttcgcactta gaaaaagcga
      121 taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca
      181 ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat
      241 atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt
      301 taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa
      361 aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata
      421 ctccacaaac catgtcc
//
LOCUS       DACDRN010000213          434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDRN010000213 DACDRN010000000
VERSION     DACDRN010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.224"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          128 a          102 c           81 g          123 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttggt cgtatcaaat gaaagcttat
      421 agaaacaaat tgaa
//
LOCUS       DACDRN010000214          430 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDRN010000214 DACDRN010000000
VERSION     DACDRN010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.225"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>430
                     /locus_tag="IWJ80_002922"
     CDS             <1..>430
                     /locus_tag="IWJ80_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9462558.1"
                     /translation="QRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIK
                     KLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKT
                     GFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          145 a           81 c           74 g          130 t
ORIGIN      
        1 caaagagcca cgtatagagc ggtttgttcc gtcttatttc ctaatggtga ctttcctagt
       61 aggagtcgat tcactcgctt aagttcaaac ttagcttaca ctatcaagat tattcgatac
      121 tttttcatca agaagctcac caaaggtgaa ctagtcggaa ttatagatag ttttcctagt
      181 ccattatgta aaccagttag aaatagacaa gcaaaactat tgaatcaaat agccaaagtt
      241 ggctataact caacaaaaaa atcttatttt tatggtctta aaattcatat gatcgttact
      301 aagactggct ttccgattac ttactcaatc acgaatccag gtgtccatga cgtgaaagtg
      361 ttagagactt tatcggaaga agcgaatctt cccaatatcc taggggacaa aggatatatt
      421 agccataaaa
//
LOCUS       DACDRN010000215          428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDRN010000215 DACDRN010000000
VERSION     DACDRN010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.226"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            82..>428
                     /locus_tag="IWJ80_002923"
     CDS             82..>428
                     /locus_tag="IWJ80_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP9462559.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHV"
BASE COUNT          150 a           78 c           84 g          116 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgt
//
LOCUS       DACDRN010000216          428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDRN010000216 DACDRN010000000
VERSION     DACDRN010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.227"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            82..>428
                     /locus_tag="IWJ80_002924"
     CDS             82..>428
                     /locus_tag="IWJ80_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP9462560.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHV"
BASE COUNT          148 a           74 c           86 g          120 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgt
//
LOCUS       DACDRN010000217          426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDRN010000217 DACDRN010000000
VERSION     DACDRN010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.228"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..420
                     /locus_tag="IWJ80_002925"
     CDS             <1..420
                     /locus_tag="IWJ80_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9462561.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          159 a           65 c           86 g          116 t
ORIGIN      
        1 tcactagatc gtttaggaag agattatgaa gatataaaaa atacagttgc ttttatgaaa
       61 cagaaaaaag tagcgttaaa aatattagat gcacagtttt tagattttaa tactggaaat
      121 gaactcctgg ataccgctat gttcgatatg tttttatcac tgctaagtta tattgcacaa
      181 aacgagcgtg agaaaattcg tgaacgtcag cggcaaggag tattattggc tcaaaaagcc
      241 ggccgatata agggacgacc aatcgagtat tctattaact caacagatcc gcaaaaacgc
      301 ttagttttta aaaccgtggt ggacatgtta aagcaaggca ttccagttgc agagattgca
      361 aaagaaaacg ggatctctcg accaactatt tataaaataa agaaagcgaa tgatttgtag
      421 aaatat
//
LOCUS       DACDRN010000218          421 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDRN010000218 DACDRN010000000
VERSION     DACDRN010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.229"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..390
                     /locus_tag="IWJ80_002926"
     CDS             <1..390
                     /locus_tag="IWJ80_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9462562.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          163 a           74 c           65 g          119 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatttattt
      421 a
//
LOCUS       DACDRN010000219          401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDRN010000219 DACDRN010000000
VERSION     DACDRN010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.denovo.230"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>401
                     /locus_tag="IWJ80_002927"
     rRNA            <1..>401
                     /locus_tag="IWJ80_002927"
                     /product="23S ribosomal RNA"
BASE COUNT          125 a           87 c          117 g           72 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt c
//
LOCUS       DACDRN010000220         3678 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.guided.129, whole genome shotgun sequence.
ACCESSION   DACDRN010000220 DACDRN010000000
VERSION     DACDRN010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.guided.129"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            149..1216
                     /gene="rlmN"
                     /locus_tag="IWJ80_002928"
     CDS             149..1216
                     /gene="rlmN"
                     /locus_tag="IWJ80_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9462563.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(1409..2512)
                     /locus_tag="IWJ80_002929"
     CDS             complement(1409..2512)
                     /locus_tag="IWJ80_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9462564.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            3065..3613
                     /gene="aac(6')"
                     /locus_tag="IWJ80_002930"
     CDS             3065..3613
                     /gene="aac(6')"
                     /locus_tag="IWJ80_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9462565.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1203 a          625 c          738 g         1112 t
ORIGIN      
        1 ttccatcttt tctaatacgg tatgatgtct tgtcttttta gtttttgtga gattaaagtt
       61 ctattcgatt tatttgtgaa gtattgcatt aaggaaatga atcatgtata gtatgtagcg
      121 aaactttgag aaaaagaggg aaaatacttt ggaaaaacag tccatttatg gcttgacgaa
      181 tgaagaatta atcaattggt ttatagaaaa cggcgagaaa aaattccgag ctgcccaagt
      241 ttgggaatgg ttgtatcaaa aacgtgtaag taatttcaca gaaatgacga atctatcgaa
      301 acaattgatc gaaaaattat cggagcattt tatcatcaat cctctaaaac agatggtcgt
      361 ccaagaagct tcggatggta cagtaaaata tttgtttgaa ctccctgata aaaacatgat
      421 tgagactgta ctgatgagac aagaatatgg cttgtcagtt tgtgtgacga cacaagtagg
      481 atgtaatatc ggctgtacgt tttgtgcaag cgggctgctc aaaaagaacc gtgatctgac
      541 tgctggagaa attgttgcac agatcatgat ggtccaacat tattttgatg agcgcaatct
      601 tggcgaacgc gtttcacatg tcgttgtaat ggggatcggt gagccatttg ataattacga
      661 caatgtaatg gactttttgc atatcatcaa tgatgccaaa ggattggcaa ttggtgctcg
      721 gcacattaca gtttccacta gtggattagc acacaaaatc aaggaattcg ctaataacgg
      781 cttgcaagtc aatctggcta tttccttgca cgcgccgaat aatgaggtgc gaacatcgat
      841 catgcggatc aaccgaagtt tcccaattga gaaattgatg gaagctgtgg atgagtattt
      901 agagaaaaca aaccgtcgaa tcacgtttga atatatcatg ttgaatcaag taaatgatag
      961 accagagcat gcgcaacaat tagcagatct attaaaagat aagaaaaaat tagcatatgt
     1021 caacttgatt ccatataatc cagtcagtga acatgatcaa tattctcgca gtccaaaagc
     1081 tgatgtattg agattttatg atgtattgaa gaaaaacggc gtaaactgtg tgatacgtaa
     1141 agaacatggt acagatattg atgcagcttg cggacaattg cgaagcaagc aaatgaaaaa
     1201 aacagttgcc aaataatcgt atcctactcg ttttttcaat taccataaaa ggagcagttt
     1261 tcttaattaa ggaactgttc aacttaaata agagaaaaga ttctatagcg aaagctataa
     1321 agcttttttt gacaagaaaa accctcatca agcaaagaaa tgtcctcaat ttgatgacag
     1381 cctgcttgat gggggtttta gcaattattt aactgatttt cttatatcct tgctggcttt
     1441 cttgtgtagc atgataagtt tctacgtcaa gaatggcctg tttaaatttt tctcgggtga
     1501 tttcataagg cacatgttta atatcatttg ttgccatggc tttatctaag attgcttcta
     1561 aatcggtcgt gacattgaga ttcatgtcgg ttaacgatac tggaagttgg ttctttttca
     1621 taaacacata gattttatca aacaaatcga actgctgatc catcatcaat aaggtcagta
     1681 caccataact gacagatgct ccgtgaaggt atccctccat tttttctaga acagtcgtac
     1741 cgtaatagaa tgagtgtgcc atagcagaat tatagttatt gttaactaaa acagaaacca
     1801 atccggtagt aacaatgata tccgaaacga cttgttcaat tgcatatgag ggacgatcgt
     1861 cggcactatc tttcaatgct tgcgtaccat aagtcaagag acgatcaatg cacatttttc
     1921 cgatttggat tcctaatcta tccgtgtagc ctagatcatc gccacgagca gaaaaagtag
     1981 attcgacttc tttggataaa gcatcgccaa tacctgccca taagtattta tcaggtgctt
     2041 cggcaataat tgctgtatca atgaaggtgt gtacaggtgg ttttgaagga aagaagagtc
     2101 cgtccattac gccttcttcg ttatagatga cacataccgc agtgacgggc gaacagttcg
     2161 aaccaatagt gggaaaggtg aagactggtt tagctagttt ttcaccaatg acttttacag
     2221 tatcgcaagc acggccacca ccgactgcaa acaggacgtc tgctttttgg acactttcat
     2281 gctggatcaa ctgttcaata tttccataag ttgaattacc cccgtaccag agactggcga
     2341 caacctctat ttcctgtgtc aaagctactt ttattttgtt ttctgccttt gaaagtgcag
     2401 tcttccctcc aatcagcact acttttgtcc catattcttt gacgattcca tttgcttttt
     2461 catacgcatc tgttccaatg gtataatttg gtaagaaaac actttgactc atattcattt
     2521 ccttctttct tcgttgaaac gggttaaaat tttaaagagt gatcgatctg actgcggctg
     2581 tagatggagt agaggtgaac ctaagacagg ataagtgatg aataaggtgg catgaacacc
     2641 ttgaaatcat caataagtat cctaaaaacg aacaagtaca aaaacattga aaacgacaat
     2701 aatgaatttt tgttcctctt agtatttcta ccgattccct ttgctttttt gtaattatct
     2761 aaaaattcta aaaattaaga ttattatatg aaaacaggtt ttatttgtca attcataatt
     2821 ggtttttttt aaagcaaaag ataatctata tcatgatgaa caaacagagt tttttcctcc
     2881 aattgatggc tttcctgatc tttcctattt tgaataaagt gtttctgtat aattttttta
     2941 ttttataagt aagataaata atgaaagaat agagatactg attggtagct aaagattttt
     3001 gagcgagaac atttcgtttt ttcaaaagga ctgttagaat aagcaagaac aagggggaag
     3061 acgtatgata atcagtgaat ttgaccgtaa taatccagta ttgaaagatc agctttctga
     3121 tttactgaga ctgacttggc cggaagaata tggagacagc tcggcagaag aagtagaaga
     3181 aatgatgaat ccagaacgaa tcgcggtagc agcggtagac caagatgagt tagtaggatt
     3241 tattggtgca atccctcaat acggtatcac aggttgggaa ttgcatccat tagttgtaga
     3301 aagctcccga cgaaagaacc aaataggtac tcgattagtc aattacttag aaaaagaagt
     3361 agcttccaga ggaggaatca cgatttattt aggtacggat gatttagacc atggaacaac
     3421 gttaagtcaa acggacctgt atgagcatac atttgataaa gtggcttcca tccagaacct
     3481 tcgtgaacat ccgtatgaat tctatgaaaa attaggttat aaaatcgtag gtgtcttacc
     3541 aaatgcaaat ggctgggaca aaccggatat ttggatggca aaaacgatta ttcctcgacc
     3601 aaattctcaa taataaaaag aggttgtgac agaagtaatc agcttcaaga aataagaagg
     3661 aatttacgaa aattactt
//
LOCUS       DACDRN010000221         3512 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.guided.131, whole genome shotgun sequence.
ACCESSION   DACDRN010000221 DACDRN010000000
VERSION     DACDRN010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.guided.131"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            554..640
                     /locus_tag="IWJ80_002931"
                     /pseudo
     CDS             554..640
                     /locus_tag="IWJ80_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance protein"
     gene            641..2581
                     /gene="tet(S)"
                     /locus_tag="IWJ80_002932"
     CDS             641..2581
                     /gene="tet(S)"
                     /locus_tag="IWJ80_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(S)"
                     /protein_id="HAP9462566.1"
                     /translation="MEEIKLKIINIGILAHVDAGKTTLTESLLYSSGAIKELGSVDSG
                     TTKTDTMFLERQRGITIQTAITSFQRENVKVNIVDTPGHMDFLADVYRSLSVLDGAIL
                     LISAKDGVQSQTRILFHALRKMNIPIIFFINKIDQNGINLPDVYQDIKDKLSDDIIIK
                     QTVNLNLKPYVIDYTEPEQWETVIVGNDYLLEKYTIGKTLNIAELEKEENERIQSCSL
                     YPVYHGSAKNNIGIKQLIEVITSKLFSPTQLNSDKLCGNVFKVEYSDDGQRLVYVRLY
                     SGTLHLRDSVNISEKEKIKVTEMYTSINGELRQIDKAEPGEIIILKNELLKLNNVLGD
                     KKRLPHREILENPLPMLQTTIEPCKSVQREKLLDALFEISDSDPLLQYYVDTVTHEIV
                     LSFLGEVQMEVTCTLIQEKYHIEIETRKPTVIYMERPLKKSEFTIDIEVPPNPFWASI
                     GLSVTPLPLGSGIQYESLVSLGYLNQSFQNAVMEGIRYGCEQGLYGWKLTDCKICFKY
                     GLYYSPVSTPADFRMLAPIVLEQAFRKSGTELLEPYLSFEIYVPQEYLSRAYNDASKY
                     CANILNTKLKGNEVILIGEIPARCIQEYRNSLTFFTNGRSVCLTELKGYQVTNIKSAF
                     QPRRPNNRIDKVRHMFNKINLH"
     gene            2669..2836
                     /locus_tag="IWJ80_002933"
     CDS             2669..2836
                     /locus_tag="IWJ80_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9462567.1"
                     /translation="MCPICGNKTRLKMREDTELKNFPLFCPKCRQEILIEITKFRITV
                     ITEPDAKTQSR"
     gene            complement(3350..>3512)
                     /locus_tag="IWJ80_002934"
     CDS             complement(3350..>3512)
                     /locus_tag="IWJ80_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9462568.1"
                     /translation="NISLDGFILGEENSHKSSKRLQVEAQLDCLDEDELVTIGKVIQA
                     IYGEKCKFN"
BASE COUNT         1265 a          531 c          582 g         1134 t
ORIGIN      
        1 tatttgatag tagatttaaa actttgcaac agaacccttt taatgatatt ttccaagtca
       61 aacttaatat taacaattca aaactattgg aaccactgca aaatattttg ttaagcagta
      121 tatatttcaa aaattatacg taatatattt gtagaaaggt tgtggacagt tgtcctattg
      181 acctgaaaag taatataata gctggatgaa taaagaatcc cttatgaact tgtaaatcgt
      241 tattaaatcg ctgaattttg atagccattc tgaagagtta tcttcaacta ctacactaac
      301 ttccattaat ttctcattaa tttccctttc gttcaaaaaa tatggttggc tttacaagca
      361 ttctatcata tgatagaatt aagataacat tcaaaaggag ataataaaaa tgcggcaagg
      421 tattcttaaa taaactgtca attagatagc gggaataatt aattaagcat ccttatttaa
      481 aataaggact ggttttttgt gcccagttta agaatacttt tatcatttta tcataaagca
      541 tctgtggcta tcagtgtgta gtatacagct atttttgtat taaaatccca gtgataagag
      601 tatttatcac tgggattttt tctgcttttt gagcttttga atggaggaaa taaaattgaa
      661 aattattaat atcggtatct tagcacatgt tgatgcagga aaaactactt tgacagaaag
      721 cttactatac agtagcggag caattaaaga gttaggaagt gtagatagcg gtacaacgaa
      781 aacggatact atgtttttgg aacgccagag aggtattact attcagaccg caataacatc
      841 ttttcaacgg gaaaatgtta aagtaaatat tgtagatact cctggacaca tggatttttt
      901 ggcagatgta taccgttcat tatctgtttt ggatggagct attttgctaa tctctgcaaa
      961 agatggagta cagtcacaaa ctcgtatact attccatgca cttagaaaga tgaacatacc
     1021 tataatattt tttattaaca aaattgatca aaatggaata aatttgccag atgtttatca
     1081 agatattaag gacaaacttt ctgacgacat cataattaag cagactgtga atctaaattt
     1141 gaaaccttat gtaatagatt atactgaacc agaacaatgg gagacagtaa ttgtgggaaa
     1201 tgattattta ttagaaaaat ataccattgg gaaaacattg aatattgcag aacttgaaaa
     1261 ggaggaaaac gaaagaattc aaagttgctc cttatatcct gtttatcacg gaagtgcaaa
     1321 gaataatatt ggaattaaac aacttataga ggtaattact agcaaattat tttcacccac
     1381 acaactcaat tcagataaac tttgtggaaa tgtttttaaa gtagaatatt cagatgatgg
     1441 tcaacggctt gtctatgtac gtctttatag tggaacgcta catttgcgag actcagtcaa
     1501 tatatcagaa aaggaaaaaa taaaagttac agaaatgtat acttcaataa atggagaatt
     1561 acgccagata gataaggcag agcctggtga gattattatt ttaaaaaatg agcttttaaa
     1621 actaaataac gtacttggag ataaaaaaag attaccacat agagaaattc ttgagaatcc
     1681 tcttcctatg ttacaaacaa caattgaacc atgtaaatca gtacaaagag aaaagttact
     1741 agatgcactt tttgaaatat ccgatagtga tccccttcta caatattatg tagatacagt
     1801 aactcacgaa attgtgctat cttttttagg tgaggtccaa atggaggtaa cttgtactct
     1861 gattcaagaa aaatatcata ttgagataga aacaagaaaa ccaactgtca tttatatgga
     1921 aagaccatta aaaaaatctg aatttaccat tgatatcgaa gtacctccaa atcctttctg
     1981 ggcttctatt ggtttatctg taacaccact tcctttgggt agtggcattc agtatgagag
     2041 cctggtttct ctaggttatt taaatcaatc atttcaaaat gcagttatgg aaggtatacg
     2101 ctatgggtgt gaacaaggat tgtacggttg gaaattaaca gactgtaaga tctgttttaa
     2161 gtatggtcta tattacagcc ctgtcagtac gccagcagat ttccgaatgc ttgcgcctat
     2221 tgtactagag caggctttta gaaagagtgg tacagagtta ttagagccat atcttagctt
     2281 cgaaatttat gtaccacaag aatatctttc gagagcatat aatgatgctt ccaaatattg
     2341 tgcaaatatt ttaaatacta agttaaaagg taacgaggtc attctcattg gtgaaattcc
     2401 agcccgttgt attcaagagt atcgaaacag tttaactttc tttacaaatg gacgcagtgt
     2461 ctgtttaaca gagttaaaag gttatcaggt tactaacatt aagtctgctt tccaaccacg
     2521 tcgtccaaat aatagaatag acaaagtaag gcatatgttt aataaaatca acttacattg
     2581 atagtgaact aaagagaaat ttttaaaatg aatattgagt tgttaattag aacaggagga
     2641 tatagatatt gtaaatacta ggtggatact gtgccctata tgtggcaata agacccgttt
     2701 aaaaatgagg gaagatactg aattaaaaaa tttccctcta ttttgtccta aatgcagaca
     2761 agaaatttta attgaaataa cgaagtttag aataactgta attacagagc cagacgcaaa
     2821 gacgcagagc cgataattcg agatatcaaa atctcatttt atcggctttt tcttttctat
     2881 gcataccatt gagcttagtt cttgatatcc tttataagaa aataagtgta ctgatttttt
     2941 ttgcagtagc tgtattacag tagacatccc tcaagcataa aaagtataca attaaaattc
     3001 aatacttgat tattgatgaa gtacagagtt actcgtcatt tcaaaactag tatatcagtc
     3061 aagtttttcc taaggaacaa aaacgaactt cgctatggga atcaaaaatt tttagtcgga
     3121 tgaagtgata tgcaataaat ccagaaaatt ttcccaacaa caaaaaatga actgtacttt
     3181 gcagcgataa gagtaaccaa agaactattt atcattcgct ctagttccta agtgtaacaa
     3241 agataaattc attttctatt tatgattaac aaaaatcaga aaaaattcat tacatctttg
     3301 taatcaatct ttcctgatag tattaattaa tccattgtgt attattgaac tagttaaatt
     3361 tacatttttc tccgtaaatt gcttgaatca ctttcccaat cgtcactaat tcatcttcat
     3421 ccagacaatc caactgtgct tctacttgaa gacgtttgga gcttttatga ctgttttctt
     3481 cccctaaaat aaatccatcc aaagaaatat tt
//
LOCUS       DACDRN010000222         3230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.guided.138, whole genome shotgun sequence.
ACCESSION   DACDRN010000222 DACDRN010000000
VERSION     DACDRN010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.guided.138"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            152..1273
                     /locus_tag="IWJ80_002935"
     CDS             152..1273
                     /locus_tag="IWJ80_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9462569.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            1531..3033
                     /gene="lsa"
                     /locus_tag="IWJ80_002936"
     CDS             1531..3033
                     /gene="lsa"
                     /locus_tag="IWJ80_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9462570.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            3100..>3230
                     /locus_tag="IWJ80_002937"
     CDS             3100..>3230
                     /locus_tag="IWJ80_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9462571.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVD"
BASE COUNT         1107 a          553 c          740 g          830 t
ORIGIN      
        1 ccggctccag ctattgcgat tttcatgttt tttctctcct ttattcaatg agttcccttt
       61 tagtatatag aaaaacagaa gaaaatagca ttgttttgtt tgaaaaaagt gaaaaaaagt
      121 gtcttactta agaagaaagt gagggatata gatggatatt ttggcggcaa tcgattcatt
      181 taaaggaagt gcttcttcat ttgaattaaa tcaggcagtt ctaaatggtt gggaagaccg
      241 taaaggaaga aaaatcaatg tgccgattgc agatggcgga gaaaatactg cagaagcaat
      301 ctattacgcg ctcgggggaa cctggcgtac gattgatggg atggatctct tatttcgtca
      361 gcgaaaaatc agttatttat tgacgaactg gcaaggaaaa aataccgcag tcattgaatc
      421 tgcacaagta ttaggattag atttacttga taaaccaacg gatcaaacca tccgacttgc
      481 ttctagttat gccttgggag agcttatcaa agatacactg cttcaagaag tggaacaaat
      541 cattgtcact ttaggaggaa gcggatgtag cgatggtggt ctaggattac tccaatcttt
      601 gggtgcttct ttagaagggt acacagaagg aaatcctttg ttatctacta gaaaacttca
      661 tttggatcga aggaacgttc ttgtttctaa agtaaaagtg atcatcgctg cagacgtgac
      721 caacccatat acaggtcaac aaggagctgc ttggcaattt ggaagacaaa aaggaggaac
      781 ggatacgacg ttagctttct tggataaaca agctgaaaag attgctcagc agataaaaga
      841 tgtttgggag gtggatttag atgacatccc aggaagcgga gcagcaggcg gtattggcgg
      901 agcccttttt ttgttaggag cgaagatggt ctctggtttt gagttggtca gccaaatcac
      961 tggattagaa aaacagatcg aacaagcgga tgtcatcatt acaggagaag ggcggattga
     1021 tcagcaaaca atccacggaa aagttccata tggcgtagca gtgcttgcaa aaaaatatcg
     1081 aaaaccaata atcgctattt gcggaagtag agaccgagag ctggaaggac tctttgattt
     1141 tctccccatt gtcttaagca ttcaattagg accaatcgat ttagaggaag caatggaaca
     1201 caggcaaaca ttagaaaaaa cagaaatctt aggacagaca ctcagcagat ttttcaacat
     1261 tctcccgaag tagttgacaa aaacaatttc tttatgctat cgtaatttca aatataggac
     1321 aaagtgaggg atccgtgcat tgaaaaatta agtcgattga tgaaagctgc attcagactg
     1381 taccttgtgg agacaaggga gcagtgtgca gattttcggg attggcttgg ttctttcgat
     1441 gtatgggtct ttttgtgtgc cccaaccctt gcatcctctt tagccagttc tgaaaatagg
     1501 actggtcttt ttttacgagg tgatggaata atgtctaaaa tcgaaataaa aaatctgaca
     1561 ttcggctatg acagccaagg cacattatta tttgaacaag ccaatctaaa ctttgacaca
     1621 caatggaaac taggacttat cggacgaaac ggtcgaggaa agacaacttt actgaatatc
     1681 ctgcaaaaca aactccctta tcaagggcaa gtgatccatc agcaagcgtt tgtctatttc
     1741 ccgcagcaaa caaaagataa agagcgtttg acctattatg tgttaaatga gattatggat
     1801 tttgagatat gggaaattga aagagagctc caactgatgc aaacagatcc tgaaatctta
     1861 tggagagaat tcagcacact atcaggagga gagaagacaa aagtcctact ggcactctta
     1921 tttgtggatg acactcattt cccgttaatc gatgaaccaa cgaatcattt ggatatctct
     1981 ggcagaaaac aagtagcggc ttacttgaaa aagaaaaaac aaggattcat cgtggtgagt
     2041 catgatcggg gatttatcga tgaagtagtg gaccatgttt tagcaatcga aaagagccaa
     2101 ctggaacttt atcaagggaa tttctctatc tatgaagaac agaaaaaact tcgtgatgaa
     2161 tttgaaatag ctaaaaatga aaaattgaaa aaagaagtca gcaggctaaa gaaaacagca
     2221 gctgaaaaag cagaatggtc tcgttcccga gaaggggaca aaacaaagaa aaaagtcgga
     2281 ttcatcgata cggaatctag gcgagtgaat aaaggagcag taggcgctga tgctgcacgg
     2341 acgatgaaac gatctaaagc aatcgtgaat cggatggaaa cccagatcag tgagaaagaa
     2401 aaattattaa aagatatcga gtatatcgat gctttgacga tgaatagcca agtgtctcat
     2461 cataaacggc tgttaagcgt agaaaatctt caattagggt atgaaaatct gttattcgag
     2521 ccagttcatt ttacgatcga acctcatcag cggatggcaa tttcaggtcc taacggcgcg
     2581 ggaaagtcat ccattatcca ttatcttctg gggacattca acggcaaggt tgtaggagaa
     2641 aaatcccagc ccaaacattt gaccattagt tacgtacgcc aaaattatga agacaatcga
     2701 gggacattgg cggaattcgc gaagaaaaac cgagtagact atcaagcatt tttgaataat
     2761 ctccgaaagc ttgggatgga aagagatgtt tttcataata aaatcgaaca gatgagtatg
     2821 ggacaacgga aaaaagtgga attggctaaa tctttatccc aaccagctga gctctatata
     2881 tgggatgaac cattgaatta tttggatgtc ttcaatcaag aacaattaga acaactgatt
     2941 ttgaacgtga aacctgctat gttactagtg gaacatgatc aaacctttct agataaagta
     3001 tctactgaga ttatttctct tgagaaaatc taaaccttgt aatctcaaag acaacaaggt
     3061 atggttaagg agaactttgt caacgaagga gagaatcaca tgctttatgt aacgagacac
     3121 ggagaaacaa cttggaacgc acaaggatta gtttgcgggc gagcagatgt gccgctgact
     3181 gaaaaaggac agatgcaggc acaggcacta gcagaaaagg tagtagattt
//
LOCUS       DACDRN010000223         3110 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.guided.140, whole genome shotgun sequence.
ACCESSION   DACDRN010000223 DACDRN010000000
VERSION     DACDRN010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.guided.140"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..172
                     /locus_tag="IWJ80_002938"
     CDS             <1..172
                     /locus_tag="IWJ80_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9462572.1"
                     /translation="GYETNPIGGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYES
                     VRLAPEKITTFE"
     gene            526..2004
                     /gene="msr"
                     /locus_tag="IWJ80_002939"
     CDS             526..2004
                     /gene="msr"
                     /locus_tag="IWJ80_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9462573.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2051..2497
                     /locus_tag="IWJ80_002940"
     CDS             2051..2497
                     /locus_tag="IWJ80_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9462574.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2696..>3110)
                     /locus_tag="IWJ80_002941"
     CDS             complement(2696..>3110)
                     /locus_tag="IWJ80_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9462575.1"
                     /translation="SQIYTTLAKLDQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWI
                     KEETPAPTQKDEFLAKIYAFSTLDKTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKK
                     QEFGRYVVIQRRIMLCQQELQWCQQVREQMQQFFE"
BASE COUNT          986 a          519 c          658 g          947 t
ORIGIN      
        1 tggctatgaa acaaatccaa ttggtggatt cgaagcagat cagttggcag aggcatttgg
       61 tttggataaa gaccgttatg ttccagttat tattttatca ataggaaaag cggtagaaga
      121 aggttacgag tctgttcgtt tagcaccaga aaagatcact acgttcgaat aaaaggtttt
      181 cagttactat tcagtagcta ttgctttttt tgaatcgtgt gtggtaacct aataaaaaag
      241 gaggtcggaa aaatgactgc atcgatgaaa ttacgtttcg aacttttgaa taacaactaa
      301 ataaattcaa aagacatgta taaacaacaa tttcatggat ggagtatgtc cattttttcg
      361 aacgacagta gaataggtga aagctatgac agatttggtg atttagtcat agttatttcg
      421 ttcagagtag gaaaagatgt gatgcagttt ttttgcaaac agatttttct gctctttttt
      481 agtatcgata attgttgttt tacatgaaat aggagtgtaa aacatatgga aaatttagca
      541 gtaaatataa caaatctaca agtcagcttt ggcaaccagc tagaactatc tattgattct
      601 cttcgtgtct atcagcaaga tcgtataggg atcataggag aaaatggtgt cggtaaatcg
      661 actttgctca aactaatagc cggtgaactt tttcccgatc atgggaaaat ccaaacagag
      721 atcaccttca actacctgcc tcaattaacc tatctcgctg aagcaaagga cctaaatttg
      781 gaattagcca gtcatttcca gttaagactg gaagaaactt cagagcggaa atggagcgga
      841 ggggaagaac gaaagatcga gttgatacgt cttctttctt cttatgaaca agggatgctt
      901 ctagacgagc caacaaccca tctagataga aaaagtattg atcgactgat tgaagagctt
      961 cgttattatt atggtacgct ggtttttgtt agtcatgatc gctattttct agatgagcta
     1021 gcatcgaaaa tctgggaagt aaaagacgga gaaatccgag agttttcggg gaattatagt
     1081 gcctatctca ctcaaaagga attggagaaa aagactcagc tacgagaagc agagtcgatc
     1141 atgaaagaga aaaaacgatt ggaaaaatcg atccaagaaa agaaaaaaca agcggaaaag
     1201 ttggaaaaag tgtccagtaa aaagaaaaag caacaaatca gaccggatcg gttgtcttcc
     1261 tctaaacaaa aagacagtgt acaaaaagcc atccaaaaga atgcgaaaac attagagaga
     1321 agactccaaa aaataggaga aacaaccaag ccgcagcaga tgaaacaaat ccgttttcca
     1381 gtaccaaaat ctcttgaact ccacagccgc tatcccatca tgggacaaaa tgtccaattg
     1441 gaaagaagtg ggagaacatt actggtaaat ggtgattttc agttttcttt aggtaaaaaa
     1501 atcgcgattg tcggtgaaaa tggttcaggt aagacaacct tattagaaca tatccgcaaa
     1561 caaggagaag gaatccttct ctctccgaaa gtaagctttc aagtatatca gcaaaagggt
     1621 tatcaaatga catctgaaga atccatcatt cgttttgtca tgagacaaac agagttttca
     1681 gaatcgcttg tccgtagttt gctgaatcac ttagggtttg ctcaggaaac tttgacgaaa
     1741 ccgttatgta cattaagtgg gggagaagcg acccgtctga cgattgcttt gctttttact
     1801 aagccaagta atgtgttgct gttagatgaa ccgactaatt ttattgatat ggcaacgatc
     1861 gaagctttag agaagctaat gcaaatatat ccgggaacga ttttgtttac ttctcatgat
     1921 tcttactttg tcgagcgtac ggctgatgaa gtttatgaaa taaaagggca gaaaataaaa
     1981 aaagtactta cgagaaattt ttaaatgtgc tacgatgatt ttcgtgacaa atttaaagga
     2041 gtggtacaga ttgcctacaa ataaaaaaga aggaataatt tttacaacaa tgatgtgttt
     2101 tttgatggtc ttggggatga gcgcgtataa tttatggctg catcaagatc ttcatttatc
     2161 aagtttgttg attggtctag tgcctggatt cgtggtggct ttcattttgg atgtcttcat
     2221 catcggtgtg ctggcgaaaa aaattgcctt taaactacca gtaaatccag aaagcaaggt
     2281 ccaaatgatc ttaactattt catgcttgat ggttttagga atggtgacat gtatgtcttt
     2341 attcggtgtg atcatggaac aaggaatacc tgatcaatta ttctctgctt atatgaatgc
     2401 ttggaaaatg aatgtgattg ccgctttacc gttgcagttg atcatcgttg gcccgctttc
     2461 aagaagtgtg ctaaaagcaa ttcaaagtaa tgaatgatgg aacactgtta tttgaaaaaa
     2521 ggccttggca tgctttttgt catagtctct tgaatgcagt tctagaaaaa tagtgatccg
     2581 aacgataggt tttggcctgt cgttcggatc gctttttctt ttccaactgt attgctcttt
     2641 tattgaagat aatttgctct tcaatagata tttctagaaa ggttctgttt atcccttact
     2701 caaaaaactg ctgcatttgt tctcttactt gctgacacca ttggagttcc tgctgacaaa
     2761 gcataattct gcgctggatc acaacataac gtccaaattc ttgtttcttg tctagcgaga
     2821 ggtcattcaa ttttttttga tttttttgca tgattttgtt cagctgttgt tctctagtga
     2881 atagcagtcc agtagctgtt gttttatcga gcgtcgaaaa agcatagatt tttgctagaa
     2941 attcatcttt ttgcgtagga gcgggagttt cctctttgat ccattcctct acaagagcac
     3001 gtccttcatc agtcaagtgg tagatctttt tttgagagtg ctcgtctttg tccaaaacgg
     3061 tcacataacc ctgctggtct aacttggcaa gcgttgtata gatctgactg
//
LOCUS       DACDRN010000224         2671 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.guided.145, whole genome shotgun sequence.
ACCESSION   DACDRN010000224 DACDRN010000000
VERSION     DACDRN010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.guided.145"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..413
                     /locus_tag="IWJ80_002942"
     CDS             <1..413
                     /locus_tag="IWJ80_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HAP9462576.1"
                     /translation="DFSLPTSFDKEPHFNQIVRHELLRMISWKVGIETGFKLSVGKNY
                     KFIERYISEDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDR
                     NITKYTRDMYKKYTGKTGCLDSTYAADIEERREQ"
     gene            410..952
                     /gene="sat4"
                     /locus_tag="IWJ80_002943"
     CDS             410..952
                     /gene="sat4"
                     /locus_tag="IWJ80_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP9462577.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            1045..1839
                     /locus_tag="IWJ80_002944"
                     /note="aph(3')-IIIa"
     CDS             1045..1839
                     /locus_tag="IWJ80_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9462578.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(1947..2135)
                     /locus_tag="IWJ80_002945"
                     /pseudo
     CDS             complement(1947..2135)
                     /locus_tag="IWJ80_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2115..2657
                     /locus_tag="IWJ80_002946"
     CDS             2115..2657
                     /locus_tag="IWJ80_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9462579.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPVLLQSFK"
BASE COUNT          899 a          437 c          627 g          708 t
ORIGIN      
        1 aagatttctc tttaccaaca tcatttgaca aagagcctca ttttaatcag attgttcgcc
       61 atgagctgct gagaatgata tcatggaagg tcggcatcga aacaggcttt aaattaagtg
      121 taggcaagaa ctataagttt attgaaaggt atatatccga ggatttgtgg gagaaacttt
      181 tgtccaccta ccggatggat tcctatgaaa acatatggga agcattattt ctatgccatc
      241 aattgttcag ggcggtatcc ggtgaggtgg cggaaaggct tcattatgcc tatccggagt
      301 atgataggaa tataacaaaa tataccaggg acatgtataa aaaatacact ggtaaaaccg
      361 gctgcctgga tagcacatat gccgctgata tagaagagag gcgggaacag tgattacaga
      421 aatgaaagca gggcacctga aagatatcga taaacccagc gaaccatttg aggtgatagg
      481 taagattata ccgaggtatg aaaacgagaa ttggaccttt acagaattac tctatgaagc
      541 gccatattta aaaagctacc aagacgaaga ggatgaagag gatgaggagg cagattgcct
      601 tgaatatatt gacaatactg ataagataat atatctttac taccaagacg ataaatgcgt
      661 cggaaaagtt aaactgcgaa aaaattggaa ccggtacgct tatatagaag atatcgccgt
      721 atgtaaggat ttcagggggc aaggcatagg cagcgcgctt atcaatatat ctatagaatg
      781 ggcaaagcat aaaaacttgc atggactaat gcttgaaacc caggacaata accttatagc
      841 ttgtaaattc tatcataatt gtggtttcaa aatcggctcc gtcgatacta tgttatacgc
      901 caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag
      961 gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg
     1021 tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa
     1081 ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat
     1141 aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg
     1201 accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct
     1261 gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag
     1321 gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc
     1381 gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat
     1441 acgaatagct tagacagccg attagccgaa ttggattact tactgaataa cgatctggcc
     1501 gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat
     1561 gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga
     1621 gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc
     1681 ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc
     1741 ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag
     1801 aaaataaaat attatatttt actggatgaa ttgttttagt acctagattt agatgtctaa
     1861 aaagctttaa ctacaagctt tttagacatc taatcttttc tgaagtacat ccgcaactgt
     1921 ccatactctg atgttttata tcttttctaa aagttcgcta gatagagttc tatattagag
     1981 atgtaaagag ttttggtgaa gaggtttgtg agtatggctt ttataaccag ggtgcaatga
     2041 gaagcactaa cagcagctag cttaagaacg cataggatat actttttatg gaagtcacag
     2101 aggaggaata aaatttgaac aggattaaca gagtaacctc tattcttatt cagctgcaat
     2161 caaaaaaaat aattcctgcc aaagaaattg cacagcgatt taatataagc ttaaggacag
     2221 tttacagaga tatccggaca cttgaagaag ccggaatacc aattggatct gaggccggaa
     2281 aaggatattt tcttgtagag ggcttcctac ttccgccggt aatgtttaca gcggcggaag
     2341 tgggtgcatt gattacagca gggaaatttt taaattgcca tggagatgaa tcatttataa
     2401 aggattttga ttcagctatg tataaaatca aatctatttt gaagcatggt gaaaaaaact
     2461 atgcacagga gttggagaac agtattaacg tgtacagcac atctggacag aaaaatacat
     2521 tggcggataa cgttattgca gcaattcaga ctgcaatctg caataaaagg gtaatatcaa
     2581 ttcaatatcc tgcatcagga gggcaagaac cggaaagcag aatgatagag ccggttctgt
     2641 tgcaaagttt taaataaaga ataaaatccc t
//
LOCUS       DACDRN010000225         2338 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.guided.156, whole genome shotgun sequence.
ACCESSION   DACDRN010000225 DACDRN010000000
VERSION     DACDRN010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.guided.156"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            146..1015
                     /locus_tag="IWJ80_002947"
     CDS             146..1015
                     /locus_tag="IWJ80_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP9462580.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            996..1730
                     /locus_tag="IWJ80_002948"
     CDS             996..1730
                     /locus_tag="IWJ80_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9462581.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1763..2302
                     /locus_tag="IWJ80_002949"
                     /pseudo
     CDS             1763..2302
                     /locus_tag="IWJ80_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
BASE COUNT          819 a          310 c          500 g          709 t
ORIGIN      
        1 atctaatcag cagaggtata aaaagacaat tgataagttt taactatctt ttgtgaaatt
       61 aaaaaaatta taaatatgtt gagtagaagc aaaagatttg gcttggaatt tttacagcag
      121 tgaatcgata aaaggagcat gcttgatggt agataatatt attaaatcag tagcagagaa
      181 attatcctct ctgtcttata tagaaggtat tgttttaggt ggttcacgtg caaggggcac
      241 ccatacagag gattcggata tagatatcgg catctattac aattcagaat catttgacat
      301 aaatactatt aatcaattcg ctacaaagct ggatgatgag catagaaata accttgttgt
      361 acctcccgga gcatggggtg attggattaa tggcggcgga tggttagtca taaacgggta
      421 tcatgtggat ttaattttac gtgatattaa acgtgtggaa caaataatga aagatacaga
      481 gcacggaatt gttactgcca attatcagac tgggcatccc catggttata ttagtgcaat
      541 gtatcgagga gaattagcga ttagcaaaat actatatgct aagaatgaaa gcttatgcga
      601 attaaaaaaa caggcagaaa cttatcccaa tgctttgcag aaaagtttag ttaacttttt
      661 tatgtttgaa gcagggttct ctttaatgtt tgtaaaagca aattcgggaa cagacgataa
      721 atattatatt gcgggtcatg tttttcgtat agtttcatgt ttaaatcaag tgttatttgc
      781 atgtaataat gcttattgta tcaacgaaaa gaaagctata aaactgcttg aaacttttga
      841 acataaacct gaaaaatata ccgagaaggt aaatcatatt tttgaagtac tcggtatctc
      901 actttttgaa tgctacgaca tgaccgagaa gctttataat gaagtgaatg aaattgtatc
      961 ggagataaat aactttttaa acgaggagag ttcagatgaa agaaaacaaa tatgatgata
     1021 atatattttt tcaaaaatac agtcaaatga gtcgctcgca gaaaggactg gctggtgcgg
     1081 gagaatggga gactttgaaa aagatgctac ctgattttaa gggtaagcgt gtgcttgatt
     1141 taggatgcgg ctatggatgg cactgtatat atgcgatgga aaacggtgct tcctctgtag
     1201 taggtgttga tatttctcat aaaatgctcg aagtagcaaa aggaaaaacc cattttccac
     1261 agattgaata tgaatgctgt gccatagaag atgtggattt cccagaggag agctttgatg
     1321 taatactaag ttcgcttgcg tttcattatg tagcagacta tgagaattta ataaaaaaga
     1381 tatataggat gctgaaggct ggtggcaatt tagtttttac agttgaacat cctgttttta
     1441 ctgctcatgg aacacaagac tggtattata acgaaaaagg agaaatactg catttcccgg
     1501 tggacaatta ttattatgag ggcaaacgga cagctatgtt tttggaagaa aaggttacaa
     1561 aatatcatag aacactgacc acatatctaa atacactgct ttcaaatagt tttataataa
     1621 atcagattgt ggagccacag ccgccagaga acatgatgga tattccgggg atggcggatg
     1681 aaatgcgacg cccaatgatg ctgattgtat cggcaaaaaa gaagatgtaa taatatagaa
     1741 aaaataaacg aggagtatgt aaatgagatc agaaaaagaa atgatggatt tagtactttc
     1801 tttagcagaa caggatgaac gtattcgaat tgtgaccctt gaggggtcac gcgcaaatat
     1861 taatatacct aaagatgaat ttcaggatta tgatattaca tattttgtaa gtgatataga
     1921 accgtttata tctaatgatg actggcttaa tcaatttggg aatataataa tgatgcaaaa
     1981 gccggaggat atggaattat tcccacctga agaaaaggga ttttcctatc ttatgctatt
     2041 tgatgattac aataaaattg atcttacctt attgcccttg gaagagttag ataattacct
     2101 aaagggcgat aaattaataa aggttctaat tgataaagat tgtagaatta aaagggacat
     2161 agttccgact gatatagatt atcatgtaag aaagccaagc gcaagggagt atgatgattg
     2221 ctgcaatgaa ttttggaatg taacacctta tgttattaaa ggattgtgcc gtaaggaaat
     2281 tttatttgct attgatcatt ttaggctcta tactttttga tgttggtgga tgacacca
//
LOCUS       DACDRN010000226         2245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.guided.160, whole genome shotgun sequence.
ACCESSION   DACDRN010000226 DACDRN010000000
VERSION     DACDRN010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.guided.160"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..439
                     /gene="thyA"
                     /locus_tag="IWJ80_002950"
     CDS             <1..439
                     /gene="thyA"
                     /locus_tag="IWJ80_002950"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9462582.1"
                     /translation="KKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQL
                     YQRSGDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLS
                     REIRAFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            459..986
                     /locus_tag="IWJ80_002951"
     CDS             459..986
                     /locus_tag="IWJ80_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9462583.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(1049..1702)
                     /locus_tag="IWJ80_002952"
     CDS             complement(1049..1702)
                     /locus_tag="IWJ80_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9462584.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            1834..>2245
                     /locus_tag="IWJ80_002953"
     CDS             1834..>2245
                     /locus_tag="IWJ80_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9462585.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEG"
BASE COUNT          786 a          417 c          456 g          586 t
ORIGIN      
        1 caaaaagact cctgattcta gaagattgat cgtttctgca tggaatccgg aagatgttcc
       61 tagcatggct ttgcctcctt gtcatacgat gtttcaattt tatgttcaag agggacggct
      121 aagctgccaa ttgtatcaac gcagtgggga tgtgtttcta ggcgttccgt tcaacattgc
      181 aagttatgcg ttgctgactc acctaattgc acatgaaaca ggtttagcgg ttggcgaatt
      241 tgttcacaca ttaggagatg ctcatcttta tatcaatcat atcgaacaaa tgaaagaaca
      301 attgagccga gaaatacgcg catttccgac tttagttttg aatcaggaaa agcagtcagt
      361 atttgatttt gagatggaag atatccaatt agaaggatat gacccacatc cggcgatcaa
      421 agctcctatc gctgtttaga atcagaaagg atgagaaaat gttagctgca atttgggcac
      481 aggatgaaaa cggattgatt ggaaaagaag accgtcttcc ttggcgtttg ccaaatgatc
      541 tgaaattctt caaacaaatg acagaagcaa atacactagt gatgggacga aaaacattcg
      601 aagggatggg tagccgtccg ttgccgaatc gtcagacgat tgttttgacg agagattctt
      661 cctataaagc agaaggcgtg catgtcatgc atagtgtgga agacgtgctt gctctggaaa
      721 aagaaacaga cggtatcttt ttcatcgctg gagggtcagc tgtttatcaa gaatttctgc
      781 cattttgtac gatattatac cgtacagtca tccatcatgt attcgatgga gatgcttatt
      841 ttccacctgt tgactggtct gattggtcac tgatcaatac tagtcaagga gaaatggatg
      901 aaaaaaatcg ttatccgcat caatttgaaa cgtatcagcg taatgagaat cctgtaaaaa
      961 gcgggacagg cgaattgacg atataaaaaa gctatggata gactagctgt tatttagagc
     1021 tgtcccatcc atagctttat tttttttatt aagtaaagaa taaaacagag aagtacataa
     1081 aacctgcacc taacataaca aacaaatgcc aaacaacatg catatagcgg atagaattca
     1141 agctataaaa gaatgcgcct atcgtgtaag aaagcccgcc tgctaccatc aaaataacac
     1201 cagtcattcc aagagagtga taaagaggaa cagcagccac gacacagagc caccccataa
     1261 aaatatagat gatcgtagat acttttgata ctctctcccg tttatgcaac gtcaacgctt
     1321 tatatataat gccaagaatc gcaaacaacc agattaatac aaataaagtc caacctagcc
     1381 atcctcggat actcagcaaa caaaaaggag tataacttcc tgcaatcaaa agaaaaatcg
     1441 aactatgatc gaatacttgg aacacctttt ttgcttttgt aaaaatcaat gaatgaaaca
     1501 aagtagacgt taaaaataaa agaatcatca tcgatccata gatagcataa gagacaatat
     1561 ggagggagga atgcaagtgg gcacctttta ctaaaagaac cactagtccc gcgatactca
     1621 atccagtccc aattccatgg gtgactgcat taaacacttc atttaatatt tcatacgttc
     1681 gattaaattt cgttttttcc atgaaaacgg ctcctttact aggtgtaatt aatgaaaaat
     1741 cattatttgt ctgttatact aaaaatagaa attgtcttac atgtagttta acatgaataa
     1801 ggcatcttgt taaagaaaga gagagagaaa ataatgacaa atgtaaaaat cgtaacagat
     1861 tcttcatgta cgatggaaaa aaatgtacgc gatgctttag gcattcgtgt catgccgcta
     1921 tccatcatga tcgacggaac agtatatccc gatgatgacc aactagacgg cgaaagattt
     1981 atggaaatga tggcgaaagc aaaaaatctg cccaaaacaa gccaaccacc aattggagaa
     2041 ttcatctcga tgtatgatga gctaggagaa gacggcagtg aagtgatttc gatccatatg
     2101 acaaaaggct tgagtggaac agtagaagca gcaagacaag ccagtaatct aacaaaaacg
     2161 aaagtaacgg ttatcgacag tgataccact gaccaaggat tatcttttca ggtaattcga
     2221 gcagctgaat tagcaaaaga aggta
//
LOCUS       DACDRN010000227         1950 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.guided.165, whole genome shotgun sequence.
ACCESSION   DACDRN010000227 DACDRN010000000
VERSION     DACDRN010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.guided.165"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            37..774
                     /gene="erm(B)"
                     /locus_tag="IWJ80_002954"
     CDS             37..774
                     /gene="erm(B)"
                     /locus_tag="IWJ80_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9462586.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            944..1204
                     /locus_tag="IWJ80_002955"
                     /pseudo
     CDS             944..1204
                     /locus_tag="IWJ80_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1367..>1950
                     /locus_tag="IWJ80_002956"
     CDS             1367..>1950
                     /locus_tag="IWJ80_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9462587.1"
                     /translation="MYQNYTINQLCLPIDLEIKLEENDFAHAIVQFVDSIPDEVFLPY
                     YQSMGRPQYHPRMLLSIILCAYIQGVYSGRQIQNMLIDSIRMRYLSQEQFPNFRTINR
                     FRVHPIMNDILDHSFVQFRELLVQSGLISGSALYIDGTKIEADANKYSFVWKKSILKY
                     KDSLDQKALENYQQMVETKILPELIDDLKDELSI"
BASE COUNT          695 a          327 c          334 g          594 t
ORIGIN      
        1 tttatttaca gatgtaataa cttcaggagt gattacatga acaaaaatat aaaatattct
       61 caaaactttt taacgagtga aaaagtactc aaccaaataa taaaacaatt gaatttaaaa
      121 gaaaccgata ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct
      181 aaaataagta aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca
      241 gaaaaattaa aactgaacat tcgtgtcact ttaattcacc aagatattct acagtttcaa
      301 ttccctaaca aacagaggta taaaattgtt gggagtattc cttaccattt aagcacacaa
      361 attattaaaa aagtggtttt tgaaagccat gcgtctgaca tctatctgat tgttgaagaa
      421 ggattctaca agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa
      481 gtctcgattc agcaattgct taagctgcca gcggaatgct ttcatcctaa accaaaagta
      541 aacagtgtct taataaaact tacccgccat accacagatg ttccagataa atattggaag
      601 ctatatacgt actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa
      661 aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cgttacttat
      721 gagcaagtat tgtctatttt taatagttat ctattattta acgggaggaa ataattctat
      781 gagtcgcttt tttaaatttg gaaagttaca cgttactaaa gggaatggag ataaattatt
      841 agatatacta ctgacagctt ccaagaagct aaagaggtcc ctagcgccta cggggaattt
      901 gtatcgataa ggggtacaaa ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc
      961 taagtgtggc aacaatattg tattaaaaaa atcgttttat ggttgttcaa attatcctga
     1021 atgtaagttt actttagctg aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa
     1081 agaattactg gagggaaaag aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc
     1141 ctacaatgcc gttgtaaaaa ttggggaaaa gggatatatt gattttatct ctttttcaaa
     1201 ataaacatgg ggctgaccca aaagtagctc ctatatgaaa aatagaggtc tttccattta
     1261 gatagtagtg gaaagacctc tatttatctt ttgaaagtga atacaaaaat aaagcacact
     1321 ctgctataat ataggtgatt aatccatttg agagaggtgc tttatcatgt atcaaaatta
     1381 taccataaac caactttgtt tgcctataga tcttgaaatt aaattagaag aaaatgattt
     1441 tgctcatgcc attgtgcagt ttgttgactc tattcccgat gaagtctttc ttccttatta
     1501 tcaatctatg ggtagacccc aatatcatcc tcgtatgcta ctttccatta ttctttgtgc
     1561 ctatattcaa ggggtttatt cgggccgcca aatacaaaat atgctgatag atagtattcg
     1621 gatgcgctac ttatctcaag agcaattccc taactttcgt actattaatc gttttcgcgt
     1681 tcatccgatt atgaacgata ttctagatca ttcatttgtt caatttcgtg agctactggt
     1741 tcaaagtggc ttaatcagtg gcagtgctct atacattgat ggtacaaaaa ttgaagcgga
     1801 tgcgaataaa tactctttcg tttggaaaaa aagtatttta aagtataagg atagccttga
     1861 tcaaaaagcc cttgaaaact accaacaaat ggtggaaaca aaaatcttac ctgagttaat
     1921 agatgattta aaggatgaac tttctataga
//
LOCUS       DACDRN010000228         1500 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.guided.177, whole genome shotgun sequence.
ACCESSION   DACDRN010000228 DACDRN010000000
VERSION     DACDRN010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.guided.177"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            228..1028
                     /gene="aph(2'')-I"
                     /locus_tag="IWJ80_002957"
                     /pseudo
     CDS             228..1028
                     /gene="aph(2'')-I"
                     /locus_tag="IWJ80_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            1012..1404
                     /locus_tag="IWJ80_002958"
     CDS             1012..1404
                     /locus_tag="IWJ80_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9462588.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
BASE COUNT          619 a          115 c          253 g          513 t
ORIGIN      
        1 gtacaagata tattaaattg atttttgaat ttttgaaaaa agaaagaaat gctaatgcag
       61 ttattttaga ccctcataaa aataatccaa gagcaataag ggcataccaa aaatctggtt
      121 ttagaattat tgaagatttg ccagaacatg aattacacga gggcaaaaaa gaagattgtt
      181 atttaatgga atatagatat gatgataatg ccacaaatgt taaggcaatg aaatatttaa
      241 ttgagcatta ctttgataat ttcaaagtag atagtattga aataatcggt agtggttatg
      301 atagtgtggc atatttagtt aataatgaat acatttttaa aacaaaattt agtactaata
      361 agaaaaaagg ttatgcaaaa gaaaaagcaa tatataattt tttaaataca aatttagaaa
      421 ctaatgtaaa aattcctaat attgaatatt cgtatattag tgatgaatta tctatactag
      481 gttataaaga aattaaagga acttttttaa caccagaaat ttattctact atgtcagaag
      541 aagaacaaaa tttgttaaaa cgagatattg ccagtttttt aagacaaatg cacggtttag
      601 attatacaga tattagtgaa tgtactattg ataataaaca aaatgtatta gaagagtata
      661 tattgttgcg tgaaactatt tataatgatt taactgatat agaaaaagat tatatagaaa
      721 gttttatgga aagactaaat gcaacaacag tttttgaggg taaaaagtgt ttatgccata
      781 atgattttag ttgtaatcat ctattgttag atggcaataa tagattaact ggaataattg
      841 attttggaga ttctggaatt atagatgaat attgtgattt tatatactta cttgaagata
      901 gtgaagaaga aataggaaca aattttggag aagatatatt aagaatgtat ggaaatatag
      961 atattgagaa agcaaaagaa tatcaagata tagttgaaga atattatcct attgaaacta
     1021 ttgtttattg atgataatat ggtttttgat attgataatt taaaaggatt tcttaatgat
     1081 accagttcat ttgggtttat agctaaagaa aataataaaa ttataggatt tgcatattgc
     1141 tatacacttt taagacctga tggaaaaaca atgttttatt tacactcaat aggaatgtta
     1201 cctaactatc aagacaaagg ttatggttca aaattattat cttttattaa ggaatattct
     1261 aaagagattg gttgttctga aatgttttta ataactgata aaggtaatcc tagagcttgc
     1321 catgtatatg aaaaattagg tggtaaaaat gattataaag atgaaatagt atatgtatat
     1381 gattatgaaa aaggtgataa ataaatgaat atagttgaaa atgaaatatg tataagaact
     1441 ttaatagatg atgattttcc tttgatgtca aaatggttaa ctgatgaaag agtattagaa
//
LOCUS       DACDRN010000229         1142 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20601_2015
            SAMN12639552-rid9883073.guided.188, whole genome shotgun sequence.
ACCESSION   DACDRN010000229 DACDRN010000000
VERSION     DACDRN010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639552
            Sequence Read Archive: SRR10030779
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742084.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742084.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,959
            CDSs (total)                      :: 2,892
            Genes (coding)                    :: 2,813
            CDSs (with protein)               :: 2,813
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 38 of 79
            Pseudo Genes (incomplete)         :: 32 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639552-rid9883073.guided.188"
                     /isolate="E20601_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            4..>1142
                     /gene="aac(6')-I"
                     /locus_tag="IWJ80_002959"
     CDS             4..>1142
                     /gene="aac(6')-I"
                     /locus_tag="IWJ80_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(6')-Ie family aminoglycoside
                     N-acetyltransferase"
                     /protein_id="HAP9462589.1"
                     /translation="MDDDFPLMLKWLTDERVLEFYDGRDKKYTLESLKKHYTEPWEDE
                     VFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTR
                     YIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCY
                     LMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFST
                     NKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYST
                     MSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIE
                     KDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII"
BASE COUNT          474 a           93 c          193 g          382 t
ORIGIN      
        1 ttaatagatg atgattttcc tttgatgtta aaatggttaa ctgatgaaag agtattagaa
       61 ttttatgatg gtagagataa aaaatataca ttagaatcat taaaaaaaca ttatacagag
      121 ccttgggaag atgaagtttt tagagtaatt attgaatata acaatgttcc tattggatat
      181 ggacaaatat ataaaatgta tgatgagtta tatactgatt atcattatcc aaaaactgat
      241 gagatagtct atggtatgga tcaatttata ggagagccaa attattggag taaaggaatt
      301 ggtacaagat atattaaatt gatttttgaa tttttgaaaa aagaaagaaa tgctaatgca
      361 gttattttag accctcataa aaataatcca agagcaataa gggcatacca aaaatctggt
      421 tttagaatta ttgaagattt gccagaacat gaattacacg agggcaaaaa agaagattgt
      481 tatttaatgg aatatagata tgatgataat gccacaaatg ttaaggcaat gaaatattta
      541 attgagcatt actttgataa tttcaaagta gatagtattg aaataatcgg tagtggttat
      601 gatagtgtgg catatttagt taataatgaa tacattttta aaacaaaatt tagtactaat
      661 aagaaaaaag gttatgcaaa agaaaaagca atatataatt ttttaaatac aaatttagaa
      721 actaatgtaa aaattcctaa tattgaatat tcgtatatta gtgatgaatt atctatacta
      781 ggttataaag aaattaaagg aactttttta acaccagaaa tttattctac tatgtcagaa
      841 gaagaacaaa atttgttaaa acgagatatt gccagttttt taagacaaat gcacggttta
      901 gattatacag atattagtga atgtactatt gataataaac aaaatgtatt agaagagtat
      961 atattgttgc gtgaaactat ttataatgat ttaactgata tagaaaaaga ttatatagaa
     1021 agttttatgg aaagactaaa tgcaacaaca gtttttgagg gtaaaaagtg tttatgccat
     1081 aatgatttta gttgtaatca tctattgtta gatggcaata atagattaac tggaataatt
     1141 ga
//
